LOCUS       DASTZR010000001         2524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1070780, whole genome shotgun sequence.
ACCESSION   DASTZR010000001 DASTZR010000000
VERSION     DASTZR010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070780"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2524
                     /gene="mfd"
                     /locus_tag="VFV20_00005"
     CDS             <1..>2524
                     /gene="mfd"
                     /locus_tag="VFV20_00005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU5286759.1"
                     /translation="RMHTAPESEPLAVVAPLRAVMEHTIQPDDLRRRGRTLRPRDRID
                     WNETAAWLFDLGYEPVTEVSEPGTFSRRGGILDVYPASATMPVRVELFGDEIETLRSF
                     DPVTQRSQEQADAVDVLPAREYSLERGPELAERLAKAGWEGVGADGEGVDLRPYAALV
                     DALREGRDVPGLDAFAAALGATASLLDHLPDRATVIFEDAVELELAHDAHEAQAEERR
                     EELHQQGLPVAAFPPPYVPRDHLERAAAERGSVRVRPDGGAVRLGWTGVTSYAGRLDT
                     FLGEVASRAAGAEDLRIVATPQAARLAELLADREVTATPHEELAEPPAPGSLVLLRVP
                     LAEGFAVPELGLRVFTDAEVFGFRKPRHPERRRRRVAMGSFLSDLKPGDHVVHEDHGI
                     ARFEQFVTREIAGVVREYLELRFAGTDVVALPTERVDKVTRYVGGTPPALSALGGREW
                     AATKRKTKKAVEEIARELIKLYALRETVHAYAFGKDTPWQIEMENAFPFTETPDQLAA
                     IEDAKRDMQRPRPMDRLVVGDVGYGKTEVALRAAFKAVQDGKQVAVLVPTTVLAQQHY
                     ETFSERLATFPVHVAMLSRFRTPAEQKEVVAGIESGDVDIVIGTHRLLQKDVRFRDLG
                     LLIIDEEHRFGVKHKERLKQLRTEVHVLSMTATPIPRTLHMALSGVRDISVIETPPES
                     RQPIETHVVERTDDILREAVLRELDRGGQVYYVHNRVQGIEHETQRLRLLVPNARFVV
                     AHGQMDERKLERVMVEFADAEHDVLVCTTIIESGLDIPNVNTIVIDHAGQLGLAQLYQ
                     LRGRVGRAAAKAYAYLLYSKQERLSEDARKRLQAVFEASELGA"
BASE COUNT          370 a          911 c          906 g          337 t
ORIGIN      
        1 gcgcatgcac accgcgccgg agtcggagcc gctcgcggtc gtcgcgcccc tgcgggcggt
       61 gatggagcac accatccagc ccgacgacct gcggcggcgc ggacgcacgc tgcgcccgcg
      121 cgaccggatc gactggaacg agaccgccgc gtggctcttc gacctcggct acgagcccgt
      181 gaccgaggtc agcgaaccgg gcacgttctc acggcgcggc ggcatcctcg acgtgtatcc
      241 cgcgagcgcg acgatgccgg tgcgcgtcga gctcttcggc gacgagatcg agaccctccg
      301 ctccttcgat cccgtgacgc agcgctccca ggagcaggct gacgccgtgg acgtcctgcc
      361 ggcgcgcgag tactcgctcg agcgcggccc ggagctcgcg gagcgcctcg cgaaggccgg
      421 ctgggagggc gtgggggcgg atggggaggg cgttgacctc cgtccctacg ccgcgctcgt
      481 cgacgcgctg cgcgagggcc gtgacgtgcc ggggctcgac gccttcgccg ccgcgctcgg
      541 tgcgaccgcg tcgctcctcg accatctgcc ggaccgcgcg accgtcatct tcgaggacgc
      601 cgtcgagctc gagctcgcgc acgacgcgca cgaggcgcag gccgaggagc gccgcgagga
      661 gctgcaccag cagggcctcc cggtcgcggc cttcccgccg ccgtacgtgc cgcgcgatca
      721 cctggagcgc gccgcggccg agcgcggcag cgtgcgcgtg cggccggatg ggggcgcggt
      781 gcgcctgggc tggaccggcg tgacctcgta cgccggacgg ctcgacacgt tcctcggcga
      841 ggtcgcgtcg cgtgccgccg gcgccgagga tctgcgcatc gtggcgacgc cgcaggccgc
      901 ccgcctcgcg gagctcctcg ccgaccgcga ggtgacggcc acgccgcacg aggagctcgc
      961 cgagccgccc gcaccgggct cgctcgtgct gttgcgtgtg ccgctcgccg agggcttcgc
     1021 cgtcccggag ctcggcctgc gtgtcttcac cgacgccgag gtcttcggct tccgcaagcc
     1081 gcggcacccg gagcgccgcc gccgccgcgt cgcgatgggc tcgttcctct ccgatctcaa
     1141 gcccggcgac cacgtcgtgc acgaggacca cggcatcgcg cgattcgagc agttcgtgac
     1201 cagggagatc gccggcgtcg tccgtgagta cctcgagctg cgcttcgcgg gcaccgacgt
     1261 cgtcgcgctc ccgaccgagc gcgtggacaa ggtcacgcgc tacgtgggcg ggacgccgcc
     1321 cgcgctttcg gcgctcggcg gccgcgagtg ggcggcgacg aagcgcaaga cgaagaaggc
     1381 ggtcgaggag atcgcgcgcg agctcatcaa gctctacgcg ctgcgtgaga cggtgcacgc
     1441 gtacgccttc gggaaggaca ccccgtggca gatcgagatg gagaacgcgt tcccgttcac
     1501 cgagacgccc gaccagctgg cggcgatcga ggacgccaag cgcgacatgc agcggccgcg
     1561 tccgatggac cgcctcgtcg tcggtgacgt gggctacggc aagaccgagg tcgcgctacg
     1621 cgcggcgttc aaggcggtgc aggacggcaa gcaggtcgcg gtgctcgtgc cgacgaccgt
     1681 gctcgcgcag cagcactacg agacgttcag cgaacgcctc gccacgttcc cggtccacgt
     1741 cgcgatgctg tcgcgtttcc gcacgcccgc ggagcagaag gaggtcgtgg ccgggatcga
     1801 gagcggtgac gtggacatcg tcatcggcac gcaccgcctg ctccagaagg acgtgcgctt
     1861 ccgcgatctc ggcctgctca tcatcgacga ggagcaccgc ttcggcgtga agcacaagga
     1921 gcgcctgaag cagctgcgca ccgaggtgca cgtcctctcg atgaccgcga cccccatccc
     1981 gcgcacgctg cacatggcgc tctccggtgt ccgcgacatc agcgtcatcg agacgccgcc
     2041 cgagtcccgg cagcccatcg agacccacgt cgtcgagcgg accgacgaca tcctgcgcga
     2101 ggcggtgctg cgcgagctcg accgcggcgg gcaggtctac tacgtccaca accgcgtgca
     2161 gggcatcgag cacgagacgc agcggctgcg cctcctcgta ccgaatgcgc ggttcgtcgt
     2221 cgcgcacggc cagatggacg aacggaagct cgagcgcgtc atggtcgagt tcgccgacgc
     2281 cgagcacgac gtgctggtgt gcacgacgat catcgagtcc gggctcgaca tcccgaacgt
     2341 gaacacgatc gtcatcgatc acgccgggca gctcggcctc gcgcagctct atcagctgcg
     2401 cggccgcgtg ggacgcgcgg cggccaaggc gtacgcgtac ctgctctatt cgaagcagga
     2461 gcgcctctcg gaggacgcgc gcaagcggct gcaggcggtg ttcgaggcga gcgagctcgg
     2521 cgcc
//
LOCUS       DASTZR010000002         2220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_365713, whole genome shotgun sequence.
ACCESSION   DASTZR010000002 DASTZR010000000
VERSION     DASTZR010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365713"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="VFV20_00010"
     CDS             <1..1082
                     /locus_tag="VFV20_00010"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5286760.1"
                     /translation="LLGRAMDEGAVGLASGLIYPPSAYGGTDELSALCEVVRAKGGLY
                     ASHIRDEGGKLLDAVEENLEIGRRSRARVQLSHHKASGQKNWGKVRESVARIEAARAS
                     GIDVQADQYPYTASSTGLAVTIPNWVHEGGSAKLTERLRDRAVRDRIRGEDTETGRAW
                     DRIVIARARHHPEYSGRSVADLARAAGKDPLEWTCDTLVEHEGAVDIIHHSMSEDDVR
                     FVMAQPWIAIGSDSRANAPYGPLSFGKPHPRSYGTFPRVLGRYARDEGLLTLEDAVRK
                     MTTLTARHLRLRDRGEVRVGAWADLVVFDPARVADAATYDDPHRYPEGIEHVVVNGTL
                     ALEQGETSNERAGRFLRLGPDLGGA"
     gene            1088..2161
                     /locus_tag="VFV20_00015"
     CDS             1088..2161
                     /locus_tag="VFV20_00015"
                     /inference="COORDINATES: protein motif:HMM:NF019375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HEU5286761.1"
                     /translation="MPKVAILAEGQFAPRTAKTAIGVLRYAPYPVVAVIDSARAGTDA
                     AAHVGVGKGVPVVADVDDAVARGAEVVLIGTAAAGGRIASELRPHIARALERGLAVWN
                     GMHERVLSDPALAAAAARGKGTVRELRDAPSDLPIGGHRTPRAGAHVVLTVGTDAAVG
                     KMTVALELVAALESLGKRAAFVPTGQTGIAIAGWGIAVDAVVADFVAGAAERMVCDAA
                     ERADWVIVEGQGSLMHPGFSGVTLGLLHGAGPELMVLCHDAARPVVKGYESEGLPVRS
                     LADCVRIYEDAASWRRPPGYPAARVAAVAVNTSAYADAVAHAAILDAESQTRLPTADP
                     IRQGRAGSERLARALADAMEARR"
BASE COUNT          305 a          778 c          854 g          283 t
ORIGIN      
        1 aactcctcgg acgcgccatg gacgagggcg cggtcgggct ggcgtccggg ctcatctatc
       61 caccgagcgc gtacggcggg actgacgagc tctcggcgct gtgcgaggtc gtgcgtgcga
      121 agggcggcct gtacgcgagc cacatccgcg acgagggcgg gaagctgctc gacgcggtcg
      181 aggagaacct cgagatcggg cggcgctcgc gcgcgcgcgt ccagctgtcg caccacaagg
      241 cctccggaca gaagaactgg gggaaggtgc gtgagtcggt cgcgcgcatc gaggccgcgc
      301 gggcgagcgg catcgacgtg caggccgatc agtacccata cacggcgtcc tcgaccgggc
      361 tcgccgtcac gatcccgaac tgggtgcacg agggcgggag cgcgaagctc accgagcggc
      421 tgcgcgaccg cgccgtccgc gaccgcatcc gcggcgagga caccgagacc ggccgcgcgt
      481 gggaccggat cgtgatcgcc cgtgcgcggc accaccccga gtacagcggc cggagcgtcg
      541 cggacctcgc gcgcgcggcg gggaaggacc cactcgagtg gacctgcgac acgctggtgg
      601 agcacgaggg agccgtcgac atcatccacc actccatgag cgaggacgac gtccgtttcg
      661 tcatggcgca gccgtggatc gcgatcggca gcgactcgcg cgcgaacgcg ccctacggtc
      721 cactctcgtt cggcaagccg cacccgcggt cgtacggcac ctttccgcgg gtcctcggcc
      781 ggtacgcgcg cgacgaaggg ctgctcacgc tcgaggacgc ggtgcgcaag atgacgacgc
      841 tgacggcgcg gcatctgcgg ctgcgcgacc gcggcgaggt gcgcgtcggc gcgtgggccg
      901 atctcgtggt gttcgatccg gcgcgcgtcg cggacgccgc gacctacgac gatccgcacc
      961 gctatccgga gggcatcgag cacgtcgtcg tgaacgggac gctcgcgctc gagcagggcg
     1021 agacctcgaa cgagcgcgcg ggccggttcc tccgcctcgg tccggatctg ggcggcgcgt
     1081 gacgactatg ccgaaggtcg ccatcctggc cgagggccag ttcgcgccgc gcaccgcgaa
     1141 gaccgccatc ggtgtgctgc ggtacgcgcc gtatccggtc gtggcggtca tcgactcggc
     1201 gcgcgccggg accgacgccg ccgcgcatgt cggcgtcggg aagggcgtgc cggtggtggc
     1261 ggacgtcgac gacgcggtcg cgcgcggagc ggaggtggtg ctcatcggga cggccgcggc
     1321 cggcgggcgc atcgcgtcgg aactccggcc gcacatcgca cgtgcgctcg agcgggggct
     1381 cgcggtgtgg aacgggatgc acgagcgcgt cctctccgat cccgcgctgg ccgccgccgc
     1441 ggcgcgcggc aaggggacgg tgcgcgagct gcgtgacgcg ccgtcggacc tgcccatcgg
     1501 cgggcaccgc accccgcggg ccggcgcgca cgtcgtgctc acggtgggca ccgacgcggc
     1561 ggtggggaag atgaccgtcg cgctcgagct cgtcgccgcg ctcgagagcc tcgggaagcg
     1621 cgccgccttc gtgccgaccg gacagaccgg catcgcgatc gccggatggg gcatcgcggt
     1681 cgacgctgtc gtcgcggact tcgtcgcggg cgcggcggag cgcatggtct gcgacgcggc
     1741 cgagcgcgcc gattgggtga tcgtcgaagg tcagggctcg ctcatgcatc ccggcttctc
     1801 gggcgtgacg ctcggcctgc tgcacggcgc aggcccggag ctcatggtcc tctgccacga
     1861 cgcggcgcgt ccggtggtga agggctacga atcggagggg ctgcccgtcc ggtccctcgc
     1921 cgactgcgtg cgcatctacg aggacgccgc gtcgtggcga cggccgccgg gctatcccgc
     1981 ggcgcgtgtc gccgccgtcg cggtcaacac ctcggcgtac gccgatgcgg tcgcgcacgc
     2041 ggcgatcctc gacgccgagt cgcagacgag gctgccgacg gccgacccga tccgacaggg
     2101 ccgcgccgga tccgagcggc tggcccgcgc gctggcggac gcgatggagg cgcggcgatg
     2161 aacacgaagg tctacacgct ggacgtgcac ctcgaggtcc ccttccagat ctcgcgcgcg
//
LOCUS       DASTZR010000003         5381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_104960, whole genome shotgun sequence.
ACCESSION   DASTZR010000003 DASTZR010000000
VERSION     DASTZR010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5381
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104960"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="VFV20_00020"
     CDS             complement(<1..799)
                     /locus_tag="VFV20_00020"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU5286762.1"
                     /translation="MIERAPFGRTGRTSPRALFGAAALSRASQEATDQAFEVLLAHGV
                     DHIDTAASYGESELRIAPVLARYGRDRFFLATKTGKRTYTDARDEIRRSLQRLGTDHV
                     DLIQLHNLVAEDDGYEQAFADDGALRAAVEARDAGLVRFIGVTGHGVQAPRQHRRSLE
                     RFPFDSVLFPYNVTQMRGDYAVDAEALIGVCESRGVAMQTIKAITLGPWRGERPATPT
                     TWYEPLSDQHDIDLAVRWVLSRPGLFLNTVGDLGLLPKVLDAASRGGP"
     gene            complement(804..1541)
                     /locus_tag="VFV20_00025"
     CDS             complement(804..1541)
                     /locus_tag="VFV20_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5286763.1"
                     /translation="MRFDGKVVLVTGADRGIGKAVVERFLTEGARLVAADLGPTGCER
                     YQNDPRVAVVLGDVTIRADAERMVGTAIERFGRLDVLVNNAGIVDRFLPVGELTDELW
                     DKVLAVNLTGPMLLSRAAIPHLTRSKGVIVNVSSVGGLFGARAGAAYVASKHGLIGLT
                     RNIAVTYASEGVRAVVVAPGGVNTGISIGGEPSARGYEALKKTLAAGVRMAEPAEIAA
                     VIAFAASSEASFVNGEVIVADGGWTAY"
     gene            1681..1866
                     /locus_tag="VFV20_00030"
     CDS             1681..1866
                     /locus_tag="VFV20_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286764.1"
                     /translation="MLLAATLAAFLSACAAYGGGTPGGGAAPNGEGAATAAPKATQPA
                     ATPKASDPKNPDDYYGY"
     gene            complement(1888..2163)
                     /locus_tag="VFV20_00035"
     CDS             complement(1888..2163)
                     /locus_tag="VFV20_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286765.1"
                     /translation="MSLIHRYTTPLADAEGRAFVVEAHGERGMNGLWEGWLEYIGVGR
                     QIVLRTDIETRQPDRRALVYWASGLRPSYLEGAFARALRARVEAFRA"
     gene            complement(2219..2518)
                     /locus_tag="VFV20_00040"
     CDS             complement(2219..2518)
                     /locus_tag="VFV20_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEU5286766.1"
                     /translation="MSFQDKTLTCRDCGQEFVWTAGEQEFYASRGLQNPPSRCPSCRA
                     ARRAGQGAGGGGGPREMFTATCSNCGGEARVPFQPRGDKPVYCSSCFEQMRGARR"
     gene            complement(2562..3245)
                     /locus_tag="VFV20_00045"
     CDS             complement(2562..3245)
                     /locus_tag="VFV20_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03717.1"
                     /note="Members of this highly hydrophobic protein
                     family,regularly are found preceded by the yybP-ykoY
                     manganese riboswitch (see RF00080). A metal cation
                     transport function is proposed; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjbE family putative metal transport protein"
                     /protein_id="HEU5286767.1"
                     /translation="MTETLQAIAAIVLIDLALSGDNALVIGMAARGLPVAQRRTAIVL
                     GGFGAVGLRVAGSAGVTLLLAIPYLQLAAAVALVVIAYRLVRPSPDHTRTVGRAATLR
                     AAILTILIADAAMSLENVLGVGAAAHGNIALLVFGLALSIPIVLFGSSFIVGLLERYP
                     RGIWLGAIALLWTAAELVLDEPTIALRHTLPWAAELLLTGVLLAMLIAYREALRYRRR
                     VDPERVRLL"
     gene            3394..3792
                     /locus_tag="VFV20_00050"
     CDS             3394..3792
                     /locus_tag="VFV20_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5286768.1"
                     /translation="MANTGSLGGIVRKQREDLRLTQAELAKLAGISRSAVSELEAGRI
                     AQPRAAVFARLAKALGIPAAVLLAAAGYPEAELLTGIEADELVILATSLTRVAGRERA
                     WLRARLLEIRDLLALRHAERARARPQHRRS"
     gene            complement(3842..4462)
                     /locus_tag="VFV20_00055"
     CDS             complement(3842..4462)
                     /locus_tag="VFV20_00055"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEU5286769.1"
                     /translation="MIGPGFLAGVGVLALLTISPGADMALVAKVTLERGRRAAFWTSV
                     GICSGLLVHATASALGLSVILATSADAFTAVKLVGAAYLAYLGVRSLRASFRPEPDPG
                     TAPPRRRGAPFLVGLLNNVLNPKVAVFYLTFLPQFIDPSHDVLAQSLLFAIAHALMGI
                     AWLSAYAYVLARLSAVFARAGVRRWLERVTGSILIALGVRLAFERR"
     gene            complement(4459..5118)
                     /locus_tag="VFV20_00060"
     CDS             complement(4459..5118)
                     /locus_tag="VFV20_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016574444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEU5286770.1"
                     /translation="MPELAPACDDVAVLIHSHDAPLDDAEWRAFLAEHDFGELIAPGT
                     RRDLPVVVPTHFVWDGANEIVLHLARANPVWAALGERPRALLSVFGAYTFIPGHWNQD
                     EYGVPTSYYAAVQLSCDAQIVDDESQIAAILERQLGHFQPEGKHAAVVPGENRYGALL
                     PSIRGIRLTVTDVRAKLKFGANRSVQHREAIAERLGERGRPLDLEARAHVLDRIARSR
                     G"
     gene            complement(5090..>5381)
                     /locus_tag="VFV20_00065"
     CDS             complement(5090..>5381)
                     /locus_tag="VFV20_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation-translocating P-type ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HEU5286771.1"
                     /translation="FATMVIGQLLLVIVERSDGYGALRTGLRGNRVIAPILVGAAGAL
                     VLVEAVPPIAALLHVATPTLDGWLVAFAVAGATTLWGELVSLLRAGARAGLR"
BASE COUNT          865 a         1863 c         1931 g          722 t
ORIGIN      
        1 tcggcccgcc gcggctcgcg gcgtcgagca ccttcggcag cagaccgaga tcgccgacgg
       61 tgttcaggaa cagcccgggg cgcgagagga cccagcgcac cgcgagatcg atgtcgtgct
      121 ggtcggagag cggctcgtac caggtcgtcg gcgtcgcggg tcgctcgccg cgccacggtc
      181 cgagcgtgat cgccttgatg gtctgcatcg cgaccccgcg cgactcacag acgccgatga
      241 gcgcctcggc atcgacggcg tagtcaccgc gcatctgcgt cacgttgtag ggaaagagca
      301 cgctgtcgaa cggaaagcgc tccaggctgc ggcggtgctg ccgcggggcc tgcaccccat
      361 gaccggtgac gccgatgaag cggacgaggc cggcgtcgcg cgcctcgacg gccgcgcgga
      421 gcgcgccatc gtccgcgaag gcctgctcgt agccgtcgtc ctcggcgacc aggttgtgca
      481 gctggatgag atccacgtgg tcggtgccga gacgctggag cgaccggcgg atctcgtcgc
      541 gcgcgtccgt ataggtgcgc ttcccggtct tcgtggcgag gaagaagcga tcgcggccat
      601 agcgtgcgag caccggcgcg atgcggagct ccgactcgcc gtagctcgcg gccgtgtcga
      661 tgtgatcgac gccgtgcgcg aggaggacct cgaaggcttg atccgtcgcc tcctggctgg
      721 cgcgcgagag cgccgccgca ccgaagagcg cgcgcggact ggtgcgaccg gtccgcccga
      781 aaggcgcgcg ctcgatcatc ggatcagtat gcggtccatc cgccgtcggc cacgatgacc
      841 tcgccgttca cgaagctcgc ctcgctcgac gcggcgaacg cgatcaccgc ggcgatctcg
      901 gcgggctcgg ccatgcgcac gcccgcggcc agggtcttct tcagcgcctc gtagccacgt
      961 gcgctgggct cgccaccgat cgagatcccg gtgttcacgc cgccgggcgc cacgacgacg
     1021 gcgcgcacgc cctcactggc gtacgtgacc gcgatgttcc tcgtgagccc gatgagaccg
     1081 tgcttgctcg cgacgtaggc cgcgccggca cgcgcaccga agaggccgcc cacggatgag
     1141 acattcacga tcacgccctt ggaccgcgtg aggtgcggga tcgcggcacg cgagaggagc
     1201 atcgggccgg tcaggttcac ggcgaggacc ttgtcccaga gctcatccgt gagctcgccc
     1261 acgggaagga agcgatcgac gatgccggca ttgttgacga ggacgtcgag gcgtccgaaa
     1321 cgctcgatcg ccgtgccgac catgcgctcc gcgtcggcgc ggatggtgac gtcaccgagg
     1381 acgacggcga cgcgcggatc gttctgatac cgctcgcatc cggtcgggcc gaggtccgcg
     1441 gcgaccaggc gcgcgccctc cgtgaggaac cgctcgacca ccgccttgcc gatccctcgg
     1501 tccgcaccgg tgaccaggac cactttgccg tcgaaacgca tggacgcacc tccgcgtcca
     1561 ctgtcgcccg cgcgctcggg ccgtcgctat ggtccgacgg cccacgccgg ggggccgttc
     1621 cgcgcgtcgt gcgcgataca tccgccacta cggcgcatgc gtatgaccga tatgcgacac
     1681 gtgctccttg cagcgacgct cgcggccttc ctctcggcgt gcgcggcgta cgggggcggg
     1741 acgcccggtg gcggtgcggc gccgaacggt gagggagccg cgaccgcggc gccgaaggcg
     1801 acacagccgg ccgccacacc gaaggcctcg gatccgaaga accccgacga ctactacggc
     1861 tattaggccg gccacgcccg cgcgacccta ggcccggaac gcctccaccc tcgcgcgcag
     1921 cgcgcgtgcg aacgcgccct cgagatagct cggacgcaga cccgacgccc agtacacgag
     1981 cgcccgccgg tccggttgac gcgtctcgat atcggtgcgg agcacgatct gtcggccgac
     2041 gccgatgtat tcgagccagc cttcccagag accgttcatg ccgcgttcgc cgtgcgcctc
     2101 gacgacgaag gctcggccct cggcatcggc gagcggtgtt gtgtaacggt ggatgaggct
     2161 cacaccctcc gtagatgcaa ccggcttgcc gcgagcgaat ttgtggccct gggccggtct
     2221 agcggcgcgc gcctcgcatc tgctcgaagc aggagctgca gtagacgggc ttgtcgccgc
     2281 gcggctggaa cggcacgcgc gcctcgccac cgcagttgct gcatgtcgcg gtgaacatct
     2341 cgcgcggtcc gccaccgccg cccgcgccct ggccggcgcg gcgagcggca cggcacgacg
     2401 ggcaccggct gggtggattc tgcagtccac gtgacgcgta gaactcctgc tccccggcag
     2461 tccagacgaa ttcctggcca cagtcgcgac aggtgagggt cttgtcttgg aagctcacga
     2521 ggctcggtcc tttctggccc cgagatcctc ggagcgcgcc gtcatagtag gcggacgcgc
     2581 tcggggtcga cgcgacggcg atagcgcagg gcttcgcggt aggcgatgag catcgcgagc
     2641 aggacgccgg tgaggagcag ctcggcagcc cacggaagcg tgtggcggag ggcgatcgtg
     2701 ggctcatcga gcacgagctc ggcggccgtc cagagcagcg cgatcgcgcc gagccagatc
     2761 ccgcgcgggt atcgctcgag caggccgacg atgaacgagc tcccaaagag cacgatcggg
     2821 atcgagagcg cgagaccgaa gacgaggagc gcgatgttcc catgcgctgc cgcgccgacg
     2881 ccgagcacgt tctcgaggct catcgcggcg tcggcgatga ggatggtgag gatggcggca
     2941 cggagcgtcg ccgcgcgccc gaccgtgcgc gtgtggtcgg ggctgggacg gacgagtcgg
     3001 tacgcgatca ccacgagggc gacggcggcg gcgagctgca gatatgggat cgcgagcagg
     3061 agcgtcacgc cggccgagcc ggcgacgcgc agtccgacgg cgccgaatcc gccaagcacg
     3121 atggcggtgc ggcgctgggc taccggaagc ccgcgcgccg ccatgccgat gacgagcgcg
     3181 ttgtcgcccg agagcgcaag gtcgatcagg acgatcgccg cgatggcctg gagcgtctcg
     3241 gtcatgcgac gagcttgcgg acgatgaccg cgacaacgcc gagcatccag aaggccgcgg
     3301 ccgcgaggta ggcagggcct gcttcggtca tccgtgacag tttactgtca ggttgtcgcc
     3361 gacgcccgtc aggcgcgata ccctattggc gtggtggcga acaccggttc gcttggcggg
     3421 atcgtccgca agcagcgcga ggacctccgc ctcacccagg ctgagctcgc caagctggca
     3481 ggcatctcgc gaagcgcggt atccgagctc gaggcgggac gcatcgcgca gccgcgtgcc
     3541 gcggtcttcg cacggctggc caaggcgctc ggcataccgg ccgcggtcct gctcgccgcc
     3601 gcgggctacc ccgaggccga gctgctcacc ggcatcgagg cggacgagct cgtgatcctg
     3661 gccacctctc tgacccgtgt ggccggacgc gagcgcgcct ggcttcgtgc gcgcctactc
     3721 gagatccgcg atctcctcgc gctccggcac gctgagcgcg cccgcgcgcg accacagcac
     3781 aggcgatcct gagcacgggc tcgcaccgtt agcggcgcgg ggctgtgatg gccggcgagg
     3841 actagcgccg ctcgaaggcg agccgaacgc cgagcgcgat gaggatggat ccggtgacac
     3901 gctccagcca gcggcgcaca cccgcacgcg cgaacacggc ggagagccgc gcgaggacgt
     3961 acgcgtaggc cgacagccac gcgatcccca tgagcgcgtg ggcgatcgcg aagagcagtg
     4021 actgcgcgag cacgtcatgg gacggatcga tgaactgcgg caggaacgtc aggtagaaga
     4081 ccgcgacctt cggattcagg acgttgttca gcagcccgac gaggaacggc gcgccacgcc
     4141 ggcgcggcgg cgcggtcccc gggtcgggct cggggcggaa cgatgcgcgc agcgagcgaa
     4201 cgccgagata cgccaggtat gccgcaccga cgagcttcac ggcggtgaag gcgtcggcgg
     4261 atgtcgcgag gatcaccgag agcccgagtg ccgacgccgt cgcgtgcacg agcagcccgg
     4321 agcagatgcc gacgctcgtc cagaacgctg cgcgcctccc ccgctcgagc gtgaccttgg
     4381 ccacgagcgc catatcggcg cccggcgaga tggtcaggag ggcgaggacc ccgacgccgg
     4441 cgaggaagcc aggcccgatc acccgcgcga gcgcgcgatg cggtcgagga cgtgggctcg
     4501 cgcctcgagg tcgagcggac ggccgcgctc cccgagcctc tccgcgatgg cctcgcggtg
     4561 ctgcacgctg cgattcgcgc cgaacttcag cttcgcccgc acgtcggtga cggtcagcct
     4621 gatgccacgg atgctcggca gcagcgcgcc gtatcgattc tcgccgggca ccaccgcggc
     4681 gtgcttgccc tccggctgga agtgccccag ctgccgctcg aggatcgccg cgatctgtga
     4741 ctcgtcgtcg acgatctgag cgtcacacga gagctggacc gccgcgtagt acgacgtcgg
     4801 cacgccgtac tcgtcctggt tccaatggcc aggaatgaac gtgtacgcgc cgaagaccga
     4861 cagcagcgcg cgtggccgtt cgccgagcgc cgcccacacc ggattggcgc gggcaagatg
     4921 cagcacgatc tcgttcgcgc catcccagac gaagtgcgtc gggacgacga cgggcagatc
     4981 gcgtcgggtg cccggcgcga tcagctctcc gaagtcgtgt tcggcgagaa aggcgcgcca
     5041 ttcggcgtcg tcgagcgggg cgtcgtgact gtggatgagc accgcgacat catcgcaggc
     5101 cggcgcgagc tccggcacgc agcaacgaaa cgagctcgcc ccacagggtc gttgcgcccg
     5161 cgacagcgaa ggcgacgagc cagccatcga gggtcggcgt cgcgacgtgc aggagggccg
     5221 cgatcggcgg gacggcctcc acgagcacga gcgcgcccgc ggcgcccacg aggatcggag
     5281 cgatcacgcg gttgccgcgc agacccgtgc gcagcgcgcc gtagccatcc gagcgctcca
     5341 cgatgactag caggagctgt ccgatgacca tggtcgcgaa g
//
LOCUS       DASTZR010000004         2920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_157080, whole genome shotgun sequence.
ACCESSION   DASTZR010000004 DASTZR010000000
VERSION     DASTZR010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157080"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /locus_tag="VFV20_00070"
     CDS             complement(<1..1340)
                     /locus_tag="VFV20_00070"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEU5286772.1"
                     /translation="MTSLLVANRGEIARRIFRTARRMGIRTIAVYSDADAALPFVGEA
                     DAAVRLGPAPARESYLDVERIIRAALDTGADLVHPGYGFLAEAPALATAVTEAGMRFV
                     GPTAQVIAALGDKAQAKEIARRAHVPILAGYAGEDQRDDAFLDAARRIGYPVMVKPVG
                     GGGGIGMQRVRDESALRDALAKARRIAATSFGDERLMIERLVERPRHVEVQIIADTHG
                     GIAALGERDCSAQRRHQKILEETPAPSLDDAQRRALAEAAIAVARETGYTNAGTVEFA
                     VDERGSFSFLEVNARLQVEHPVTELVWDVDLVEHQLRVALGERLALKEPAPRGHAIEV
                     RLYAEDVEAGFLPSTGPLLHVRWPDGIRVDAGYAEGDAIGVHYDPLLAKVIAWAPTRS
                     DAAAALAGALASARIHGVGTNRDMLVRILRHPAFLDGALAAFLDTHGADTSAPLA"
     gene            complement(1337..2116)
                     /locus_tag="VFV20_00075"
     CDS             complement(1337..2116)
                     /locus_tag="VFV20_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU5286773.1"
                     /translation="MADDLVRFERDGRGVVWITLARPERRNAFDAAIIAGVVAALGKV
                     DLSARAVVLAAEGDAFCAGADLGWMKSMVDYGLEQNMADSRALAEMFRALDGLPMPLL
                     ARVQGPAIGGGAGLVAMADIAVASTEAVFGFSEVRLGIIPAVISPYVVRKIGVGYATA
                     LFVTGMRFDAARAQQIGLVEAIEAPDELDAKLELYIDAVVSGGPHAVNAAKRIVREVA
                     GRPIDEVRDLTIERIAALRVSAEGQEGMRAFLERRPARWDT"
     gene            complement(2191..2562)
                     /locus_tag="VFV20_00080"
     CDS             complement(2191..2562)
                     /locus_tag="VFV20_00080"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU5286774.1"
                     /translation="MQNGVPERPIDPAAVAAAEQNMLSARDERRMRQLLEALCDPTRV
                     KIVQALTDTTLAAGDLAHVIGRSRSATSQHLKVLRDLGAVVASRNGNVVRYSVATGLT
                     GTVVANAVAAFQDLRNSTSGD"
     gene            2690..>2920
                     /locus_tag="VFV20_00085"
     CDS             2690..>2920
                     /locus_tag="VFV20_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286775.1"
                     /translation="MPRVLVLDNDVAIAELLRAVLEDGGHEAIVSSDADELPALDIDC
                     VVTDLMSVTLYSRDDAREWILRLRDRYPTAPIV"
BASE COUNT          410 a         1078 c         1018 g          414 t
ORIGIN      
        1 gccagcggcg ccgacgtgtc ggctccgtgc gtgtccagaa acgccgccag ggcgccgtcg
       61 aggaacgccg ggtggcgaag gatgcgtacc agcatgtcgc ggttggtccc gacgccgtgg
      121 atccgcgccg atgcgagcgc acccgcgagt gcggctgcgg cgtcggaccg cgtcggcgcc
      181 caggcgatca ccttggccag cagcggatcg tagtggaccc cgatcgcgtc gccctcggcg
      241 tagcccgcgt cgacgcggat gccatccggc cagcggacgt gcagcagcgg cccggtcgac
      301 ggcaggaacc cggcctcgac gtcctccgcg tagagccgta cctcgatcgc gtggccgcgc
      361 ggcgcgggtt ccttgagcgc cagccgttcc ccgagggcga cgcgaagctg gtgctccacg
      421 agatcgacgt cccacacgag ctcggtgacc ggatgctcga cctgaaggcg cgcgttcacc
      481 tcgaggaacg agaacgagcc acgctcgtcc accgcgaact ccaccgtgcc cgcgttcgtg
      541 tacccggtct cgcgcgcgac ggcgatggcc gcctccgcga gcgcgcgccg ctgcgcatcg
      601 tcgagcgagg gcgccggcgt ctcctcgagg atcttctggt ggcgccgctg cgccgagcag
      661 tcgcgctcgc cgagcgcggc gataccgccg tgcgtgtcgg cgatgatctg cacctccacg
      721 tgccgtggcc gctcgacgag ccgttcgatc atgagccgct cgtcgccgaa cgacgtggcc
      781 gcgatgcgcc gtgctttcgc gagcgcgtcg cggagcgcgg attcgtcgcg cacgcgctgc
      841 atgccgatac cgccgccgcc gccgacgggc ttgaccatga ccgggtagcc gatccgccgc
      901 gccgcgtcga ggaatgcgtc gtcgcgctga tcctcgcccg cgtagcccgc caggatgggc
      961 acgtgcgcgc gccgcgcgat ctccttcgcc tgcgccttgt cgcccagcgc cgcgatgacc
     1021 tgcgccgttg gcccgacgaa gcgcatgccg gcttcggtga cggccgtcgc gagggcgggt
     1081 gcctccgcga ggaagccgta gcccggatgc accaggtcgg cgccggtgtc gagcgccgcg
     1141 cggatgatgc gctccacgtc gaggtaggac tcgcgcgcgg gcgccggccc gaggcgcacg
     1201 gcggcgtcgg cctcgccgac gaacggcagc gcggcgtccg cgtcggagta caccgcgatg
     1261 gtgcggatcc ccatgcgccg cgccgtccgg aagatccgcc gcgcgatctc gccgcgattc
     1321 gcgacgagga gcgaggtcac gtatcccagc gcgcgggacg gcgctccagg aacgcgcgca
     1381 tgccctcctg gccctcggcg gaaacgcgca gcgcggcgat acgttcgatg gtcaggtcgc
     1441 gcacctcgtc gatcggccgg ccggccacct cgcgcacgat gcgcttcgcg gcgttcaccg
     1501 catgcggtcc gcccgagacg accgcgtcga tgtagagctc gagcttcgcg tccagctcgt
     1561 cgggcgcctc gatcgcctcg acgaggccga tctgctgcgc gcgcgccgcg tcgaagcgca
     1621 tgcccgtcac gaagagcgcg gtcgcgtatc ccacgccgat cttccgcacg acgtacggcg
     1681 agatcaccgc ggggatgatg ccgaggcgca cctcgctgaa accgaagacc gcctcggtgg
     1741 acgccaccgc gatgtcggcc atcgccacga gccccgcgcc gccgccgatg gccggtccct
     1801 gcacccgcgc gaggagcggc atcggaaggc cgtcgagcgc gcggaacatc tccgcgaggg
     1861 cacgggagtc ggccatgttc tgctcgaggc cgtagtcgac catgctcttc atccagccca
     1921 ggtccgcgcc cgcgcagaac gcgtcgccct cggccgcgag gacgacggcg cgagcggaga
     1981 ggtcgacctt tccgagcgcc gcgacgacgc cggcgatgat cgccgcgtcg aacgcgttgc
     2041 gccgctccgg acgcgcgagc gtgatccaca ccaccccacg cccatcgcgt tcgaacctga
     2101 ccagatcgtc cgccatctgt gccgtgagtg tgccgctaat gagcctcgaa cgccccggcg
     2161 tgggggacgg agcctagcgg ccgggtcgca tcagtcccca ctcgtcgaat ttcgcaggtc
     2221 ctggaaggcc gccaccgcgt tggcgacgac cgtaccggtc agtccggtgg cgacggagta
     2281 gcgcactacg ttgccgttgc ggctcgcgac gacggcgccg aggtcgcgca gcaccttgag
     2341 atgctggctc gtggcggagc ggctgcgccc gatgacgtgc gcgaggtcgc ccgccgccag
     2401 cgtcgtgtcc gtgagcgcct ggacgatctt cacgcgcgtc gggtcgcaca gcgcctcgag
     2461 cagctggcgc atgcgccgct cgtcgcgcgc ggaaagcatg ttctgctccg ccgccgcgac
     2521 ggccgccggg tctatcggcc gctcgggcac gccgttctgc atttgaccac caacctctcg
     2581 ctggaagtga ggacagctat gcatccgcca taccgtccac aacgccggta acgattgcgc
     2641 aacggcgcaa gttgcgattg accgggcgcc accgccacgc tctccgcgcg tgccacgggt
     2701 gctggttctc gacaacgacg tcgcgatcgc tgagctgctg cgcgccgtgc tcgaagacgg
     2761 gggtcacgag gccatcgttt cgagcgatgc ggacgagctc cccgccctcg acatcgactg
     2821 cgtggtgacg gacctcatga gcgtgaccct gtattcgcgc gacgacgccc gggagtggat
     2881 cctccgcctg cgcgatcgct acccgaccgc gccgatcgtc
//
LOCUS       DASTZR010000005         3419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1149622, whole genome shotgun sequence.
ACCESSION   DASTZR010000005 DASTZR010000000
VERSION     DASTZR010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149622"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VFV20_00090"
     CDS             <1..732
                     /locus_tag="VFV20_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU5286776.1"
                     /translation="GARIMSVVNVVGSEATRVSDDVIYLHTGPEISVASTKAYTAMLV
                     DLYLFAIYLAQHRGTIDPDASAKLLAEAFHLPTLMDAVLREEPKLRALAYRYHDAANF
                     LHLGRGVNYPTALEGALKLKELSYIHAEGTAAGEMKHGTNALIDKTLPVVAIALQDSV
                     YKKMLSNIQEVRARAGIVIALATDGDQEITKYADEVIRIPATNELLSPALAVVPLQLL
                     AYHIADKRGNDVDQPRNLAKAVTVE"
     gene            804..1184
                     /gene="acpS"
                     /locus_tag="VFV20_00095"
     CDS             804..1184
                     /gene="acpS"
                     /locus_tag="VFV20_00095"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEU5286777.1"
                     /translation="MSRDPASEVGIDIIETVRIKGVLERHDTRFLGRVYTEWEQRYCR
                     HNVLHLAGRWAAKEAVSKVLGLGVRGVGWREIEIKRTPMGQPTVTLHGRAEQRARALG
                     LREPLSVSISHIRDLAVAVAVGLR"
     gene            1214..2134
                     /locus_tag="VFV20_00100"
     CDS             1214..2134
                     /locus_tag="VFV20_00100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEU5286778.1"
                     /translation="MRSVLRPLPAEAGKEARGRVLIVGGSQRYPGAMLLAGRAAARCG
                     AGVVTVAAARSVVEMIAGRDANITFFPLAEAQPGVVATGAAAQLSPILGDKIRALLVG
                     PGLAHAAGTDDFVVAVLRNARSVTTVVDADGLNALARTEDWRQSLPPRTILTPHDGEA
                     ARLSGGVVPRDAARVAFAERHATQWGVTLVLKGAVTVVTDGKRTYVHDAPNPTLGVGG
                     SGDALGGAIAAFAARGLAPLAAAAAGVWVHGRAGALLAGEFGESGALATDIVDALPRA
                     LREIVGVQRQEKTAEVRRLRPAARAPRRPR"
     gene            2137..3303
                     /gene="alr"
                     /locus_tag="VFV20_00105"
     CDS             2137..3303
                     /gene="alr"
                     /locus_tag="VFV20_00105"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU5286779.1"
                     /translation="MLAPLRSTVVDVDLDAIVDNYRALRARAANAEVIAVVKADAYGH
                     GIEAVAETLASAGVAMLAVVTVEEALSIRASGVRAPVLVLVGATETSEIEAAVAADCA
                     VVVWDVAQAKLVGDVARAIGRRANVHVKVDTGLTRLGAPLAEAAARYRAIRGVAGLNV
                     EGLFTHLATADEPDVTSDREQLARFAEVLRAIGDAPRYVHAAASAGVAAFGAIPGCTA
                     IRPGLSLYGLHAAAHLEPALALRPALSWRSRVRRVADAKKGTGVSYGLEYRLPRDGRI
                     ATVPVGYGDGLPRAVGKRGRLIVGGRALPFAGRICMDLVMLDVTDVAVSEGDEVVVIG
                     RQGDAAQTAEALAAAAGTINYEIVTGIRRRVPRRYQRAGEVVAQRTLLDGYVRL"
BASE COUNT          464 a         1220 c         1275 g          460 t
ORIGIN      
        1 ggcgcgcgga tcatgtccgt cgtcaacgtc gtcggcagcg aggcgacgcg cgtctccgat
       61 gacgtcatct acctgcacac cggaccggag atcagcgtcg cgtcgacgaa ggcgtacacc
      121 gcgatgctcg tcgacctcta cctgttcgcg atctacctcg cgcagcaccg cggcacgatc
      181 gatcccgacg cgtccgcgaa gctgctcgcc gaggcgttcc acctgcccac gctcatggac
      241 gcggtgctgc gcgaggagcc gaagctgcgc gcgctcgcgt accggtacca cgacgccgcg
      301 aacttcctcc acctcgggcg cggcgtgaac tacccgaccg ccctggaggg cgcgctcaag
      361 ctgaaggagc tctcgtacat ccacgccgag ggcacggccg cgggtgagat gaagcacggc
      421 acgaacgcgc tcatcgacaa gacgctcccg gtcgtggcca tcgcgctcca ggactccgtc
      481 tacaagaaga tgctctcgaa catccaggag gtacgcgcac gcgccggcat cgtgatcgcg
      541 ctcgccaccg acggcgacca ggagatcacg aagtacgcgg atgaggtcat ccgcatcccg
      601 gccacgaacg agctgctgtc gccggcgctc gccgtggtgc cgctgcagct cctcgcgtac
      661 cacatcgccg acaagcgcgg caacgacgtg gaccagccgc gcaacctcgc gaaggcggtg
      721 acggttgaat agcactacga gggagggctt tgcccgaccg agtcccgtac cggcccccac
      781 gaacggagca gcggagctgc gaagtgagta gggatccagc gagtgaagtt gggatcgaca
      841 tcatcgaaac ggtgcggatc aagggcgtgc tggagcggca cgacacccgc ttcctcggcc
      901 gggtatatac ggagtgggag cagcgctact gccggcacaa cgtcctgcat ctcgcggggc
      961 gctgggcggc gaaggaggcg gtctcgaagg tgctcggcct cggtgtgcgc ggcgtcggct
     1021 ggcgcgagat cgagatcaag cgcaccccca tgggccagcc caccgtcacg ctgcatggcc
     1081 gcgcggagca gcgcgcgcgc gcgctcggac tgcgcgagcc gctgtccgtc agcatctcgc
     1141 acatccgcga tctcgccgtc gcggtggcag tgggactgcg ctgaccgagc ccctgccgct
     1201 cgacgcgagc gtcgtgcggt cggtgcttcg gccgctgccg gccgaggcgg ggaaggaggc
     1261 gcgcggacgc gtcctcatcg tcggcggatc gcagcggtac cccggtgcga tgctgctcgc
     1321 cggccgcgcc gcggcgcgct gcggggccgg ggtggtcacc gtcgcggcgg cccgctcggt
     1381 cgtcgagatg atcgccggtc gcgatgcgaa catcacgttc ttcccgctgg ccgaggcgca
     1441 gccgggcgtg gtcgccacgg gcgccgccgc gcagctctcg ccgatcctcg gcgacaagat
     1501 ccgcgccctg ctggtcggcc cgggtcttgc gcatgccgcg gggaccgatg acttcgtcgt
     1561 cgcggtcctg cggaacgcgc ggagcgtaac caccgtggtg gacgccgacg gcctgaacgc
     1621 gctcgcgcgg acggaggact ggcggcagtc gctgccgccg cggacgatcc tcacgccgca
     1681 cgacggggag gccgcgcgcc tgtccggcgg cgtggtgccg agggacgccg cgcgcgtcgc
     1741 gttcgccgag cgccacgcga cgcagtgggg cgtgacgctc gtgctgaagg gcgcggtgac
     1801 ggtggtgacc gacggcaagc gcacctacgt ccacgacgcg ccgaatccca cgctcggcgt
     1861 gggcggatcg ggcgacgcgc tgggcggcgc gatcgccgca ttcgcggcgc gcggcctcgc
     1921 gccccttgcc gccgccgccg ccggcgtctg ggtgcacggt cgcgcggggg cgctgctcgc
     1981 cggcgagttc ggcgagtcgg gtgcgctcgc gacggacatc gtggacgcgc taccgcgcgc
     2041 gctgcgcgag atcgtcggcg tccagcgtca ggagaagacc gccgaggtgc ggcgcctgcg
     2101 accggccgcg cgcgccccac ggcggccgcg ttgagcgtgc tcgcgccgct gcgctcgacc
     2161 gtggtcgacg tcgacctcga cgccatcgtc gacaactacc gcgcgctgcg cgcgcgcgcc
     2221 gcgaatgccg aggtgatcgc cgtcgtgaag gccgacgcgt acggccacgg gatcgaggcg
     2281 gtcgcggaga cgctcgcgtc cgcgggcgtc gcgatgctcg cggtcgtgac ggtcgaggag
     2341 gcgctctcga tccgtgcgtc cggcgtgcgg gctcccgtgc tcgtgctggt cggcgcgacc
     2401 gaaacgtccg agatcgaggc cgccgtggcg gccgactgcg ccgtcgtcgt ctgggatgtc
     2461 gcgcaggcga agctcgtcgg cgacgtcgcg cgtgccatcg gacgccgcgc gaacgtgcac
     2521 gtgaaggtgg acaccgggct cacgcgcctc ggcgcgccgc tcgcggaggc ggcggcgcga
     2581 taccgcgcca tccgcggggt cgccggcctc aacgtggagg gcctctttac gcacctggcc
     2641 accgcggacg agccggacgt cacgagcgat cgggagcagc tcgcgcgctt cgccgaggtg
     2701 ctgcgcgcga tcggggacgc gccacgctac gtgcacgccg ccgcgagcgc tggtgtcgcg
     2761 gcgttcggag cgatcccggg ctgcaccgcg atccggccgg gtctttcgct gtacgggctg
     2821 cacgcggccg cgcacctcga gccggcgctg gcgctccgtc ccgcgctcag ctggcgctcg
     2881 cgcgtgcgcc gcgtcgccga cgcgaagaag gggacgggcg tctcgtacgg actcgagtac
     2941 cggctgcccc gcgacgggcg catcgccacg gtcccggtgg gctacggcga cggactgccg
     3001 cgtgcggtgg ggaagcgcgg acggctcatc gtcggcggcc gggcgctgcc gttcgccgga
     3061 cggatctgca tggacctcgt catgctcgac gtcacggacg tggcggtgag cgagggggac
     3121 gaggtcgtcg tgatcggtcg tcagggcgac gcggcgcaga cggccgaggc gctcgccgcg
     3181 gccgccggta cgatcaacta cgagatcgtc actggcatcc gccggcgcgt cccgcgccgc
     3241 tatcagcgcg ccggcgaggt cgtggcccag cgcacgctcc tggacggata cgtgcggctg
     3301 tgaacatcgc tcggcggctc gcgatcctcg tcgcgatcct cgtcgtcctc tcggcgtgca
     3361 tcccgccgca ggtcgagcca tcgccgtcgc cgagcgcgag cggcagcccg agcgcgagc
//
LOCUS       DASTZR010000006         5436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_261771, whole genome shotgun sequence.
ACCESSION   DASTZR010000006 DASTZR010000000
VERSION     DASTZR010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261771"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VFV20_00110"
     CDS             complement(<1..214)
                     /locus_tag="VFV20_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286780.1"
                     /translation="MNVRSYVLIVAALGAVSVAQAARTVRLEGVDLLMLAILVTLAAV
                     TQRMPVFLFRSSAISISFAAVIASYVL"
     gene            complement(438..1448)
                     /locus_tag="VFV20_00115"
     CDS             complement(438..1448)
                     /locus_tag="VFV20_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEU5286781.1"
                     /translation="METGLALVIVVVILGVVFEYVNGFHDAANAIATVVATRVLTPTQ
                     AVIMAGSLNLIGALSGVAVAKTVGAGIVDAKIVTQDLVVAALIAAIAWDLLTWWLGLP
                     TSSSHALIFSIIGASVAVHGVGVVIVPGLEKTSFGIVYSPALAFLAGYLIMLALYWIL
                     RRASWGGVQRFFGKAQILSSAYMAFSHGGNDGQKTMGIIALALAAYGVVPTGDAFFIP
                     DWVKWTCAVAIGVGTATGGWRIIRTMGTRITVLTPVHGFAAETTAGTVIEVATRFGIP
                     ISTTHAISGAILGVGSTRRLSAVRWGIASRIVTAWVLTIPGCFVLGYAVAVIFRAIGI
                     RA"
     gene            complement(1458..2078)
                     /locus_tag="VFV20_00120"
     CDS             complement(1458..2078)
                     /locus_tag="VFV20_00120"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEU5286782.1"
                     /translation="MPSFSLIPRKNIFFTQFTQHAQNALEAAQALDALFNDFTDIERK
                     VRDIHAIEHYGDKLTHEIMRELNETFVTPLDREDIIGLASKIDDVTDVAYDVSELVHL
                     YKVKTVRPAAARQTKALVSAAMEMVAMMENLQSLKNLEQHWIKLNTFENEGDQIFREA
                     VAELFDKETNAIELIKWKDIHSLIEIALDRCEDVANIVETIKIKHS"
     gene            complement(2254..3351)
                     /locus_tag="VFV20_00125"
     CDS             complement(2254..3351)
                     /locus_tag="VFV20_00125"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5286783.1"
                     /translation="MNPKRVLAVAQRIALGFRRDHRSLALLIVAPLLVLSLVGAVWGS
                     TAQTIPNITFAADRFGLPQPVATRLIEALTKSDAIHGTTGTFDDGMRDLRDGKTDGVV
                     WLDGSTIHIRIEGSDPLRSGGVAGGVQKALGEAVGSLGQAAANAGVGTPKVEIDQLYG
                     AADYTLLDYLAPVLVAFFAFFFIFILSAVSFLRERTSGTLERLMATPLRRGELVVGYL
                     AGFSFFALIQAVVIILFTVYVLNVHYRGNLLTIFLVEAVMVVGAVSIGLLVSAAAHNE
                     LQAVQFVPIVLLPQVFLSGLLIPVSQLPDWLRPFAGVLPLTYANEALKAVMIKGAEIS
                     DPIVLRDIGVLTLFGVLMAAGAVASIRREVA"
     gene            complement(3348..4106)
                     /locus_tag="VFV20_00130"
     CDS             complement(3348..4106)
                     /locus_tag="VFV20_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5286784.1"
                     /translation="MNSTTSAIHASDVRKRFGPVEALAGIDVEVRAGEIYGLLGPNGS
                     GKTTFIRCLAGLLRPDAGSLTVLGRRPRETVSAGRVGYMTQAAALYGDLSVTENLAFF
                     ARLEGLPNAAQRIESALRTVDLLDRRRSTVSTLSGGMRQRVSLAAALLHEPDLLLLDE
                     PTVGVDPVLRHEFWSHFRALAASGVTLLISSHVMDEASRCDRLGLIRAGRVLAEGSAS
                     ELVARAGTKDLESAFLALAGPEAQASGAGEGRRA"
     gene            complement(4120..4695)
                     /locus_tag="VFV20_00135"
     CDS             complement(4120..4695)
                     /locus_tag="VFV20_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018352714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEU5286785.1"
                     /translation="MPPLGRRPGASGSRGAILRAARRHFAEVGYDRATIRRIATGAGV
                     DPALVLHFYGSKEELFSAAMDFPRPADVLPRVFGPGVEGLGERVTRFFLETWESDAGE
                     GLLGLLRSLHTSERAAVMMREFVSRELLARVAPTLSGRDRELRATLAVSHLIGLAVMR
                     YVVRLEPLASASPAALARRIGPTIQRYFDGP"
     gene            4798..4989
                     /locus_tag="VFV20_00140"
     CDS             4798..4989
                     /locus_tag="VFV20_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286786.1"
                     /translation="MEAFVFGLLIGVAFGVIVVGFLAVGAYDRGFNEALHRRKAWRAE
                     LVARQAAAQRVLPAMRKAS"
     gene            5197..>5436
                     /locus_tag="VFV20_00145"
     CDS             5197..>5436
                     /locus_tag="VFV20_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286787.1"
                     /translation="MAVSLASLALLVAGSAPATATHAWGNYHWSRAANPFNVALGDNL
                     TPDWDGYLVTASGDWSQSTVLDTSIQPGSTTGKRCR"
BASE COUNT          915 a         1848 c         1895 g          778 t
ORIGIN      
        1 acagcacgta gctggcgatg accgcggcga aggagatcga gatcgccgag gagcggaaca
       61 ggaagaccgg catgcgctgg gtcaccgcgg cgagggtcac caggatcgcg agcatcagca
      121 ggtccacgcc ctccaggcgc acggtccggg cggcctgcgc caccgagacc gcgcccagcg
      181 cggcgacgat gaggacgtac gacctcacgt tcaccgtgcg caccccagac gacgcgcgat
      241 gcggaccacc tgctcccaga cctcccctgt gtccacgcgg agatagccga tattccgcac
      301 acgtttgagc cgatggtcat cacccgccgg agtgacggcg gaaatatttg ctgtacggat
      361 acgcggccga cactgcggct acgtcattca gatgacgcga gccaggtgcg gagcgcgctc
      421 cgcacgccgg ccgtcggcta tgcccggatg ccgatcgcgc ggaagatgac agcgaccgcg
      481 tagccgagca cgaagcagcc ggggatggtg aggacccacg ccgtgacgat gcggctggcg
      541 atgccccagc ggaccgccga gagccggcgg gtcgacccaa cgcccaggat cgccccggag
      601 atcgcgtgcg tcgtcgagat cgggataccg aatcgcgtgg ccacctcgat gaccgtgccg
      661 gcggtcgtct ccgcggcgaa gccgtgcacc ggggtcagca ccgtgatccg cgtgcccatc
      721 gtccggatga tccgccagcc gccggtcgcg gttccgacac cgatggccac ggcgcacgtc
      781 cacttcaccc agtccgggat gaagaacgcg tcgccggtcg ggacgacgcc gtacgcggcg
      841 agcgcgagcg cgatgatgcc catcgtcttc tggccatcgt tgccgccgtg gctgaaggcc
      901 atgtacgcgc tcgagaggat ctgtgccttg ccgaagaagc gctgtacgcc gccccacgag
      961 gcgcggcgga ggatccagta gagcgcgagc atgatgagat agccggccag gaacgccagc
     1021 gcgggcgaat agacgatgcc gaaggacgtc ttctcgaggc cgggcacgat gaccacgccc
     1081 acaccgtgca ccgccacaga ggcgccgatg atcgagaaga tcagcgcgtg gctggacgag
     1141 gtcggcaggc cgagccacca ggtgagcaga tcccaggcga tcgccgcgat gagcgcggcg
     1201 acgaccagat cctgcgtgac gatcttcgca tcgacgatcc cggcgccgac ggtcttcgcc
     1261 accgccacgc ccgagagcgc gccgatgagg ttcaggctgc cggccatgat gaccgcctgc
     1321 gtcggcgtga gcacgcgggt cgcgacgacg gtcgcgatcg cgttcgcggc gtcgtggaag
     1381 ccgttcacgt attcgaagac gacaccgagg atgacgacga cgatgacgag ggcgaggcct
     1441 gtttccaact tcttccccta cgagtgcttg atcttgatgg tctcgacgat gttggccacg
     1501 tcctcgcagc ggtcgagcgc gatctcgatc agcgagtgga tgtccttcca tttgatgagc
     1561 tcgatcgcgt tcgtctcctt gtcgaagagc tcggcgaccg cctcgcggaa gatctgatcg
     1621 ccttcgttct cgaaggtgtt gagcttgatc cagtgctgtt cgaggttctt gaggctctgc
     1681 aggttctcca tcatggcgac catctccatc gccgcggaca cgagcgcctt cgtctggcgg
     1741 gccgccgccg gccgcaccgt cttcaccttg tagaggtgga cgagctccga cacgtcgtag
     1801 gcgacgtcgg tcacgtcgtc gatcttcgat gcgagcccga tgatgtcctc gcggtcgagc
     1861 ggtgtgacga acgtctcgtt gagctcgcgc atgatctcgt gcgtgagctt gtccccgtag
     1921 tgctcgatgg cgtggatgtc gcgcaccttc cgctcgatgt cggtgaagtc gttgaagagc
     1981 gcatcgagcg cctgggccgc ctcgagcgcg ttctgtgcgt gctgcgtgaa ctgcgtgaag
     2041 aagatgttct tccgcgggat cagcgagaag ctgggcattc ggtgcctcct cttccagaca
     2101 caaaaagacc gcgccgatgg cgcggtccac atgattcgca tgatgtacgc acgccgctcc
     2161 ccggcgctcg gaccccgcag ggtccaccct cagacttcat ccgtagagca cgataccgga
     2221 agccgacgcg acgcgctcac ccggtaaggg ggtctacgcg acctcccgcc tgatggaggc
     2281 gacggctccc gcggccatga ggaccccgaa gagggtgagc acgccgatat ctcgcaggac
     2341 gatagggtcc gagatctcgg cgcccttgat catcaccgcc ttcagcgctt cgttcgcgta
     2401 cgtgaggggc aggaccccgg cgaagggccg gagccagtcc ggcagctgcg acacggggat
     2461 cagcagcccc gagaggaaca cctgcggcag gagcacgatc ggcacgaact gcaccgcctg
     2521 cagctcgttg tgcgcggcgg ccgatacgag cagcccgatg gacaccgcgc ccacgaccat
     2581 caccgcctcg acgaggaaga tcgtcagcag gttgccccgg tagtgcacgt tcagcacgta
     2641 caccgtgaac aggatgatca ccaccgcctg gatgagcgcg aagaagctga agcccgcgag
     2701 gtagccgacg acgagctcgc cgcgacgcag cggcgtcgcc atgagccgtt cgagcgtgcc
     2761 gctggtgcgc tcgcgaagga aggagacggc ggacaggatg aagatgaaga agaaggcgaa
     2821 gaacgcgacg agcaccggcg cgaggtagtc gagcagcgtg tagtcggccg ctccgtagag
     2881 ctggtcgatc tcgactttgg gcgtgcccac gccggcgttc gccgcggcct ggccgaggct
     2941 gccgacggcc tcgccgagcg ccttctgcac gccgccggcg acgccgccgc tgcggagcgg
     3001 gtccgagccc tcgatccgga tgtggatcgt ggagccgtcg agccagacca caccgtcggt
     3061 cttgccgtcg cgcagatcgc gcatcccgtc gtcgaacgtg ccggtcgtgc cgtggatcgc
     3121 gtccgacttc gtgagcgcct cgatgaggcg cgtggccacc ggctgcggaa ggccgaaccg
     3181 atcggcggcg aaggtgatgt tggggatcgt ctgtgccgtc gatccccaca ccgcaccgac
     3241 gagcgacagg accagcagcg gcgcgacgat gagcagcgcg agcgagcgat ggtcccggcg
     3301 gaagccgagc gcgatccgct gcgcgaccgc gagcacgcgc ttcgggttca cgcccgcctc
     3361 ccttcacccg cgccgctcgc ctgtgcttcc ggaccggcga gcgcgaggaa cgcggactcg
     3421 aggtccttcg tgcccgccct cgcgacgagc tccgacgcgc tgccctcggc caggacgcgg
     3481 cccgcgcgga tcaggccgag ccggtcgcag cgagaggcct cgtccatgac gtggctcgag
     3541 atgagcagcg tcacgccgct ggccgcgagc gcgcggaagt gggaccagaa ctcgtggcgc
     3601 aggacgggat cgacccccac ggtcggctcg tcgagcagca ggagatcggg ctcgtgcagg
     3661 agggcggcgg cgagcgatac acgctgccgc atcccgccgg acagcgtcga gacggtcgag
     3721 cgccggcgat ccagcaggtc caccgtccgg agggcggact cgatgcgctg cgccgcgttc
     3781 ggcagtccct cgagccgcgc gaagaacgcg aggttctccg tcaccgagag gtcgccgtac
     3841 agcgccgcgg cctgcgtcat gtagccgacg cgtccggccg acacggtctc ccgcggcctc
     3901 cggccgagca ccgtgagcga gcccgcgtcg ggccgcagca ggccggcaag acagcggatg
     3961 aacgtcgtct tgcccgagcc gttcggaccg agcagaccgt agatctcgcc ggcgcggacc
     4021 tcgacgtcga tgcccgcgag cgcctcgacc gggccgaacc gcttccggac gtcgctcgcg
     4081 tggatcgcgc tcgtcgttga attcatcgcc cggtgaattc taaggcccgt cgaaataccg
     4141 ctggatggtg ggcccgatgc gccgggccag cgcggcaggg gaggccgagg cgagcggctc
     4201 gaggcgtacg acgtagcgca tgacggcgag tccgatgagg tggctcacgg caagggtcgc
     4261 gcgcagctcg cggtcgcgcc cggagagcgt gggcgccacg cgcgccagca gctcgcggga
     4321 cacgaactcg cgcatcatca ccgccgcccg ctcgctcgtg tgcagcgagc ggagcaggcc
     4381 gagcagaccc tcgccggcgt cggattccca ggtctcgagg aagaagcgcg tgacgcgctc
     4441 gccgagaccc tccacgccgg gaccgaacac gcgcggcagc acgtcggccg gccgcgggaa
     4501 gtccatggcc gcgctgaaca gctcctcctt ggagccgtag aagtggagca cgagcgccgg
     4561 atcgacgccg gcgccggtgg cgatccggcg gatggtggcg cggtcgtatc cgacctcggc
     4621 gaagtggcgg cgagcggcgc ggaggatcgc cccgcggctg ccgctggccc ccggccggcg
     4681 gcccagagga ggcatccggc caagcgtagc gacgcctccc gcgacgtggc atggcggttg
     4741 cttagcgagc gaccggatac ggccatcgag gtggcggaga gaggacagtc aagacagatg
     4801 gaagcgttcg ttttcgggct tctgatcggc gtcgcgttcg gcgtcatcgt ggtcggcttt
     4861 ctcgcggtcg gcgcctacga ccgcggcttc aacgaggcgc tccatcgtcg caaggcatgg
     4921 cgcgcggagc tcgtcgcgcg gcaggccgcc gcgcagcgcg tcctcccggc gatgcggaag
     4981 gccagctagc gccggccgcc gccgtcttct aaaggtcgca aggggccgcc gatgggcggc
     5041 ccttgcgttt gccggtcagt gggagcagcc gcatagccac cgggatcacg cagaccgcga
     5101 cgcacctgac tctttacccc ggtcgcccgc gcacccatca tccccgggat agcccggtca
     5161 ccagccgggc accgggaggg gaccatgcgg cgcttcttgg cagtctcgct cgcgtctctc
     5221 gcgttgctcg tcgcaggcag cgcgccggcg accgcgacgc acgcgtgggg caactaccac
     5281 tggtctcgcg cagccaaccc attcaacgtc gcgctcggcg acaacctgac gccggactgg
     5341 gatggctacc tcgtcacggc atcgggcgac tggagccagt cgaccgtgct ggacacgtcc
     5401 atccagcccg gctccaccac cggcaagcga tgccgg
//
LOCUS       DASTZR010000007         2153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1045210, whole genome shotgun sequence.
ACCESSION   DASTZR010000007 DASTZR010000000
VERSION     DASTZR010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045210"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="VFV20_00150"
     CDS             <1..885
                     /locus_tag="VFV20_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016330127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HEU5286788.1"
                     /translation="SVAVQTGVDGIDILFGTSSYLREFSHGKSVDDIIAEAKQVITFI
                     KDHGKEVRFSSEDSFRSTEGDLLKVYKAVDSFGVDRVGVADTVGIATPRQCYALAHEL
                     RHHVTCDIEFHGHNDAGCAIANSYSILEGGATHIDTSVLGIGERNGITPLGGFVARMY
                     ADDPEAIRRKYDLRKLKDLDEMIASMVGIEIPYNNFVTGKYAFHHKAGMHTKAIYINP
                     NSYEILDPGDFGLERTMNIAHRLTGHNAVGKRAEQLGLTFGKAELRDITRKIKAMADA
                     GPLSMEQLDAVLKEWVVA"
     gene            882..>2153
                     /locus_tag="VFV20_00155"
     CDS             882..>2153
                     /locus_tag="VFV20_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEU5286789.1"
                     /translation="MTGKSLAEKILSRSSGRDVRAGDLVIVPLSRVMVHDSVIDAVMQ
                     GLRDLGKERVWDTSRVCVFVDHAAPAPTPVVADSHRVLREWVREQKIATFYDAGEGVC
                     HQIMVEEGYCEPGTVIVGSDSHSNSYGAVGAFGAGMGATDIAVALALGRTWLRVPESI
                     KVTFTGTLRKGITMKDAIMRVVREVGTDGARYACVEFHGVGGLPQGDRITLAGMTTEM
                     GAKAGIVVGTPEAPDWLWPDPDASYVKSVTVDLSTLEPQIAVPPRVDQVVDISAAVGQ
                     PVDIVFLGSCTNGRLVDMQQAASILRGRRIDPRVRLEVVPSSRRQFELAMQDGTLQLL
                     SAAGAVIGSSGCGPCLGRTGGVLAGEEVCLSTANRNYKGRMGSPQANIYLGSPLVAAV
                     AALTGVIDDPRAYLEASEAEFEALVERRRAER"
BASE COUNT          356 a          726 c          745 g          326 t
ORIGIN      
        1 agcgtcgccg tgcagaccgg cgtcgacggc atcgacatcc tcttcgggac atcgtcctac
       61 ctacgggagt tctcgcacgg caagagcgtc gacgacatca tcgccgaggc gaagcaggtc
      121 atcacgttca tcaaggacca cggcaaggag gtgcgcttct cgagcgagga ctccttccgc
      181 tcgaccgagg gcgacctgct gaaggtctac aaggccgtcg attcgttcgg ggtggaccgc
      241 gtcggcgtcg ccgacacggt gggcatcgcg acgccgcggc agtgctacgc gctggcgcac
      301 gagctccgcc accacgtcac gtgcgacatc gagttccacg ggcacaacga cgcgggatgc
      361 gcgatcgcca actcgtacag catcctcgag ggcggcgcga cgcacatcga cacgagcgtc
      421 ctcggcatcg gcgagcgcaa cgggatcacg ccgctcggcg gattcgtcgc gcgcatgtac
      481 gccgacgacc cggaggcgat ccgcaggaag tacgacctgc gcaagctcaa ggatctcgac
      541 gagatgatcg cgtcgatggt cggcatcgag atcccgtaca acaacttcgt caccggcaag
      601 tacgccttcc accacaaggc aggcatgcac acgaaggcca tctacatcaa tccgaactcg
      661 tacgagatcc tcgatccggg cgatttcggc ctcgagcgca ccatgaacat cgctcaccgc
      721 ctgaccggtc acaacgcggt ggggaagcgt gccgaacagc tggggctcac gttcggcaag
      781 gcggagctcc gcgacatcac ccggaagatc aaggcgatgg ccgatgccgg gccgctgtcg
      841 atggagcagc tggacgcggt attgaaggag tgggtcgtcg catgaccggg aagtcgctcg
      901 ccgagaagat cctctcgcgg tcctccggcc gcgacgtccg cgcgggcgac ctcgtcatcg
      961 tcccgctgtc gcgcgtcatg gtccacgact cggtgatcga cgcggtgatg cagggcctcc
     1021 gggacctcgg caaggagcgc gtgtgggaca cgtcacgggt gtgcgtcttc gtcgaccacg
     1081 ccgcgccggc gccgacgccg gtcgtggcgg actcccatcg cgtgctccgc gagtgggtcc
     1141 gcgagcagaa gatcgcgacg ttctatgacg cgggtgaggg cgtctgccac cagatcatgg
     1201 tcgaggaggg ctactgcgag cccggcaccg tgatcgtcgg ttcggattcg cacagcaact
     1261 cgtacggggc cgtcggcgcg ttcggcgcgg ggatgggagc gacggacatc gccgtcgcgc
     1321 tcgccctcgg ccggacctgg ctgcgcgtgc ccgagtcgat caaggtcacc ttcaccggga
     1381 cgctccgaaa gggcatcacc atgaaggacg cgatcatgcg cgtcgtccgc gaggtcggca
     1441 ccgatggggc gcggtatgcg tgcgtcgaat ttcacggcgt gggcggtctc ccgcagggcg
     1501 atcgcatcac gctcgcgggc atgacgacgg agatgggcgc caaggcgggc atcgtcgtcg
     1561 gaacgcccga ggcaccggac tggctgtggc cggatccgga cgcgtcgtac gtgaagagcg
     1621 tgacggtcga cctctcgacg ctcgagccgc agatcgcggt gccgccccgc gtcgaccagg
     1681 tcgtggacat ctccgccgcc gtcgggcagc ccgtggacat cgtgttcctc ggctcctgca
     1741 cgaacggccg gctcgtggac atgcagcagg cggcgagcat cctgcgcggg cgacgcatcg
     1801 acccgcgcgt gcggctcgag gtcgtgccgt cctcccgccg ccagttcgag ctcgcgatgc
     1861 aggacggcac gttgcagctc ctgtcggccg cgggcgcggt catcggctca tccggttgcg
     1921 gcccgtgtct cggccgcacc ggtggcgttc tggccggcga ggaggtctgc ctctcgaccg
     1981 ccaaccgcaa ctacaagggc aggatgggct cgccgcaggc gaacatctac ctcggctcgc
     2041 cgctcgtcgc ggccgtggcg gcgctcaccg gcgtgatcga tgacccgcgc gcatacctcg
     2101 aggcgtccga ggctgagttc gaagcgctcg tcgagcgccg ccgcgccgag cgg
//
LOCUS       DASTZR010000008         3212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_940876, whole genome shotgun sequence.
ACCESSION   DASTZR010000008 DASTZR010000000
VERSION     DASTZR010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940876"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..1049
                     /gene="nuoH"
                     /locus_tag="VFV20_00160"
     CDS             93..1049
                     /gene="nuoH"
                     /locus_tag="VFV20_00160"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEU5286790.1"
                     /translation="MTELLIATVESIALVLIVFTVAAYAILGERRIIARMQARIGPDR
                     VGPQGVFQPLADILKLLSKEDIRPVAADKVLHYLTPILTLVPVMLVFAVIPAAPGIHL
                     TDLNIGYLYVAAVSSIAVYGVALGGWSSNSKYALLGGLRSSAQLISYELAQGLAALGV
                     IAAAGSLSLVRIVDAQFPVPFAVVQPLGLAVYLLASIAESSRAPFDLPEAEQELGAGY
                     HTEYSSFRFALFMLSEYVHVIVSSALVATLFLGGWWPFAGGVLGVAAFAAKMALLIFI
                     FVWLRATLPRVRYDTLMAFGWKVLIPLALIQLAVVAIWVALR"
     gene            1046..1516
                     /gene="nuoI"
                     /locus_tag="VFV20_00165"
     CDS             1046..1516
                     /gene="nuoI"
                     /locus_tag="VFV20_00165"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEU5286791.1"
                     /translation="MSVIDDILAIAKGMGVTLRTAFRAPVTLRYPEERAVLPDRHRGR
                     HELERHENGLEKCVGCELCALVCPADAIYVEGAENPKDAPVSIGERYAKTYVINLLRC
                     IYCGYCVEACPTEALQMKKEFELAAPTKAQLIVSKEELLATLPKREPEDSWLPG"
     gene            1530..2057
                     /locus_tag="VFV20_00170"
     CDS             1530..2057
                     /locus_tag="VFV20_00170"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEU5286792.1"
                     /translation="MNAEQAIFAVAGPICLVAAASAVTRRDPLSGALSLVITLFSLAV
                     LFLGLGAPFLAGVQVAVYAGAIMVLLIFVIMLLGVAHTDLPRLRRPALQLAGPLVALA
                     LFGILAVAILRGGLPPAATDALPEDNVAAVADLLFGRYAFAFEAASVLLLAALVSAVV
                     VARKRSSEREWPGRR"
     gene            2057..2356
                     /gene="nuoK"
                     /locus_tag="VFV20_00175"
     CDS             2057..2356
                     /gene="nuoK"
                     /locus_tag="VFV20_00175"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019738313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEU5286793.1"
                     /translation="MPTEAYLLLGALLFALGAAGVITRTNALSVLIAVELMLNAVNLT
                     FVAAARRYAQLDGAIVAFFVITVAAAEVAVGLGIVIDLARRKTSVDVDDASELRG"
     gene            2353..>3212
                     /locus_tag="VFV20_00180"
     CDS             2353..>3212
                     /locus_tag="VFV20_00180"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEU5286794.1"
                     /translation="MTLLLLVALPLAGAVIIALFGGLMGRRTIGAVATAALTASFGAG
                     VALAQAFAAGKTSLVAEIGPWLPIRGADITLRLDPPMVPLALMVAGVSALICLYSIGY
                     LAHDGGAQRYFAALDLFVFAMLLIVVASNLLVLFAGWELVGLCSYLLIAHWRERPAAA
                     AAGVKAFVVNRVGDAAFLVGIFMLFVAYHTVDLAEIAGRVQPAAQTLAPSANATLLIA
                     SALLLAGALAKSAQLPLHVWLPDAMEGPTPVSALIHAATMVTAGVVLLLRLASILHPT
                     VLAGAAIIGAT"
BASE COUNT          385 a         1162 c         1094 g          571 t
ORIGIN      
        1 cgcgcgttcc tcgccgagaa cgcgcccggc gttccgacga gccgactgct ggcgtggcac
       61 gacccgcagc cgcgcgtgga ggtcacgccc gagtgaccga gctgctcatc gcgacggtcg
      121 agtccatcgc gctcgtgctc atcgtgttca cggtcgccgc gtacgcgatc ctcggcgagc
      181 gacggatcat cgcccggatg caggcgcgca tcggacccga ccgcgtcggt ccgcagggcg
      241 tgttccagcc gctcgcggac atcctcaagc tgctcagcaa ggaggacatc cgtcccgtcg
      301 ccgcggacaa ggtgctccat tacctcacgc cgatcctcac gctcgtgccg gtgatgctcg
      361 tgttcgccgt catcccggcg gcgccgggca tccatctcac cgatctgaac atcggctacc
      421 tgtacgtcgc ggcggtctcg tcgatcgcgg tgtacggggt cgcgctcggc ggctggagct
      481 cgaacagcaa gtacgcgctg ctgggtggcc tgcgctcgag cgcccagctc atctcctacg
      541 agctcgcgca ggggctcgcg gccctcggcg tgatcgccgc cgcggggtcg ctctcgctcg
      601 tccgtatcgt cgacgcgcag ttcccggtcc cgttcgcggt cgtgcagccg ctgggactcg
      661 ccgtgtacct gctcgcgagc atcgcggagt cgagccgcgc gccgttcgac cttcccgaag
      721 cggagcagga gctcggcgcc ggctaccaca ccgaatactc atcgttccgg ttcgcgctct
      781 tcatgctctc cgagtacgtc cacgtcatcg tctcgtccgc gctcgtggcc acgctgttcc
      841 tcggcgggtg gtggcccttc gccggcggag tgctcggcgt cgccgcgttc gccgcgaaga
      901 tggccctcct gatcttcatc ttcgtctggc tgcgtgcgac gctcccacgt gtccgctacg
      961 acacgctgat ggccttcggg tggaaggtgc tcatcccgct ggcgttgatc cagctcgccg
     1021 tcgtcgcgat ctgggtggcg ctgcgatgag cgtcatcgac gacatcctcg ccatcgccaa
     1081 agggatgggc gtgaccctgc ggaccgcgtt ccgcgctccc gtgacgctgc gttaccccga
     1141 ggagcgcgcg gtcctgcccg accggcaccg cgggcggcac gagctcgagc gtcacgagaa
     1201 cgggctcgag aagtgcgtcg ggtgcgagct ctgcgcgctg gtgtgcccgg ccgacgcgat
     1261 ctacgtcgag ggagcggaga accccaagga cgcgccggtc tcgatcggcg agcgctacgc
     1321 gaagacctac gtcatcaacc tcctgcgctg catctactgc gggtattgcg tcgaggcgtg
     1381 cccgaccgag gcgctccaga tgaagaagga gttcgagctc gccgcgccga cgaaggcaca
     1441 gctcatcgtc agcaaggagg agctgctggc gacactgccg aaacgcgagc ctgaggactc
     1501 ctggctgccg ggctaggctc ggtgacgtca tgaacgcgga gcaggcgatc ttcgccgtcg
     1561 ccggaccgat ctgccttgtg gcggccgcca gcgcggtcac gcgacgcgat ccgctctcgg
     1621 gggcgctgag cctggtgatc acgctcttct cgctcgccgt gctgttcctc gggctcgggg
     1681 cgccgttcct cgcgggcgtg caggttgccg tgtacgcggg cgccatcatg gtgctgctga
     1741 tcttcgtgat catgctgctc ggcgtcgcac acacggatct cccgcggctg cgcaggccgg
     1801 cactccagct cgccggtccc ctggtggccc tcgcgctctt cggcatcctc gcggtggcga
     1861 tccttcgcgg cggcctgccg cccgccgcca cggacgcgct cccggaggac aacgtggccg
     1921 ccgtcgcgga tctcctgttc ggccggtacg cgttcgcgtt cgaggccgcg agcgtgctgc
     1981 tcctcgcggc gctcgtgtcg gccgtggtcg tcgcgcgcaa gcgctcgtca gagcgcgaat
     2041 ggccgggtcg ccgctgatgc ccaccgaggc gtacctgctc ctcggcgcgc tgctgttcgc
     2101 gctcggtgcg gcgggcgtga tcacgcggac gaacgctctc tctgtgctca tcgccgtcga
     2161 gctgatgctg aacgccgtca acctgacgtt cgtcgccgcg gcgcgccgct acgcgcagct
     2221 ggacggcgcg atcgtcgcgt tcttcgtgat caccgtcgcg gccgcggagg tcgccgtcgg
     2281 tctcggcatc gtcatcgacc tcgcgcggcg caagacatcg gtggacgtcg acgacgcctc
     2341 ggagctgcgc ggatgacctt gttgctgctg gtcgcgctcc cgctcgcggg agcggtcatc
     2401 atcgcgctct tcggcgggct catgggtcgc cgcacgatcg gcgccgtcgc caccgccgcg
     2461 ctgacggcgt ccttcggcgc cggcgtcgcg ctggcccaag ccttcgcggc ggggaagacc
     2521 tcgctcgtcg cggagatcgg tccctggctg cccatccgcg gcgcggatat cacgctgagg
     2581 ctcgatccgc ccatggtgcc gctcgcgctc atggtggcag gcgtgtccgc gctcatctgc
     2641 ctgtactcga tcggctacct ggcgcacgac ggcggggcgc agcgctactt cgcggcgctc
     2701 gacctcttcg tgttcgcgat gctgctcatc gtcgtcgcgt cgaacctgct cgtgctcttc
     2761 gcgggatggg agctcgtcgg cctctgctcg tacctgctca tcgcccactg gcgcgagcgg
     2821 ccggccgcgg ccgcggcggg cgtgaaagcg ttcgtcgtga accgcgtggg ggacgccgcg
     2881 ttcctcgtcg gcatcttcat gctcttcgtc gcgtatcaca cggtggacct ggcggagatc
     2941 gcgggccgtg tccagcccgc ggcgcagacg ctcgcaccga gcgccaatgc gacgctcctc
     3001 atcgcgagcg cgctgctgct ggccggcgcg ctggcgaagt ccgcgcagct gccgctgcac
     3061 gtgtggctgc ccgacgcgat ggaggggccg acacccgtct ccgcgctgat ccatgcggca
     3121 acgatggtca ccgcgggcgt cgtgctgctc ctgcgactcg cgagcatcct gcatcccacc
     3181 gtcctcgccg gcgccgcgat catcggcgcg ac
//
LOCUS       DASTZR010000009         2241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_131557, whole genome shotgun sequence.
ACCESSION   DASTZR010000009 DASTZR010000000
VERSION     DASTZR010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131557"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1428)
                     /locus_tag="VFV20_00185"
     CDS             complement(<1..1428)
                     /locus_tag="VFV20_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEU5286795.1"
                     /translation="MSDPILDPLNPEQRAAVTHKDGPLLILAGAGSGKTRVLTHRIAY
                     LIREGVPARAILAVTFTNKAAREMRDRLDRLVGKERLAELTVGTFHAFCARLLRHDGP
                     LVGLDRGFAIYDETDQRAILRQAMGDQGVSEKLFSPGAIGATISGAKNEMKGPADLAQ
                     QPRNQLERVTAMVWPRYESLLRENNAVDFDDLLLLACRLFATSDRALERWQDRYRYIL
                     VDEYQDTNRAQYELLRYLAGLERNLAVVGDDDQSVFSWRGADVRNILDFERDYPNAKV
                     VKLEQNYRSTQRILDAAWSIVRNNAARKEKKLWTERVGGPDVVVLQAYDEHHEAELIA
                     REIERLRRQGEVKTAKDVAVLYRTNAQSRPIEDVFRSFGLAYQVVGGVSFYQRREVKD
                     VLAYLRLVRNPQDSVALARVLNVPPRGIGDKTRSSLLSFAREQGVGVAEALLRGEEIA
                     AVPARQRATLASFGRLLERLRSDAAA"
BASE COUNT          319 a          757 c          772 g          393 t
ORIGIN      
        1 cgcggccgcg tcggaccgca gccgctcgag gaggcgcccg aacgacgcca gcgtcgcgcg
       61 ctggcgggcc gggaccgcgg cgatctcctc cccccgcagc agcgcctcgg cgacgccgac
      121 gccctgctcg cgagcgaacg agagcaggct cgagcgcgtc ttgtcgccga tcccgcgcgg
      181 cgggacgttg aggacgcgcg caagggcgac cgagtcctgc gggttccgca ccagccgcag
      241 atacgccagc acgtccttca cctcgcggcg ctggtagaag gacacgccgc cgacgacctg
      301 ataggcgagt ccgaacgagc ggaacacgtc ctcgatcggc cgtgactgcg cattcgtccg
      361 gtagaggacc gcgacgtcct tcgcggtctt gacctcgccc tgccgccgca ggcgctcgat
      421 ctcgcgcgcg atgagctcgg cctcgtggtg ctcgtcgtac gcctgcagca cgacgacgtc
      481 tggaccgccg acgcgctcgg tccagagctt cttctccttg cgcgccgcgt tgttgcggac
      541 gatggaccac gccgcgtcga ggatgcgctg ggtgctgcgg tagttctgct cgagcttcac
      601 gaccttcgcg ttcgggtagt cgcgctcgaa gtcgaggatg ttgcgcacgt ccgccccgcg
      661 ccacgagaac acgctctgat cgtcgtcgcc cacgacggcc aggttgcgtt ccagccccgc
      721 gaggtagcgc agcagctcgt actgcgcacg gttggtgtcc tggtactcgt cgacgaggat
      781 gtagcggtag cggtcctgcc atcgctcgag cgcgcggtcg ctggtcgcga acagtcggca
      841 tgcgagcagc agcagatcgt cgaagtcgac cgcgttgttc tcgcggagca acgactcgta
      901 tcggggccac accatcgcgg tgacgcgctc cagctgattc ctcggctgct gggcgagatc
      961 cgccggaccc ttcatctcgt tcttcgcgcc cgagatcgtg gcgccgatgg cgcccggcga
     1021 gaacagcttc tcgctcacgc cctgatcgcc catggcctgc cggaggatcg cgcgctggtc
     1081 ggtctcgtcg tagatcgcga agccgcgatc gagcccgacc agggggccgt cgtgccgcag
     1141 gagcctggcg cagaaggcgt ggaacgtccc gaccgtcagc tccgcgaggc gttccttccc
     1201 gaccaggcgg tcgagccggt cgcgcatctc gcgcgccgcc ttgttcgtga aggtcacagc
     1261 gaggatcgcg cgtgccggca cgccttccct gatgagatag gcgatgcggt gcgtgaggac
     1321 gcgcgtcttc cccgagccgg cgcccgccag gatcaggagc gggccgtcct tgtgggtgac
     1381 agccgcacgc tgctcgggat tgagggggtc gaggatgggg tcggacacca cctcaattgt
     1441 ggacgcgaat gtggataaag gcctttcgcc cggcggggac gcggcgcact cttcacggcc
     1501 tatcccgaga agctgtcgtg gggcgatctc catcggccca cgccggagtc tcgggattct
     1561 gtttgtccac cgatctgctc gccgtccggc gcggcgtgtg cggcgacggc gagagcggcg
     1621 accgaaaccc tctcttcgcg gggtatcccg tcgctcgtcg cgacgggaac cctccccgcg
     1681 cgggggccct cggtcgccgc tctcgcctac acccgttcct tagccgttcc ttggtcgcgc
     1741 cccgcgtcgg ttgctgttgg cgctcggtgg acaagtgatt ccctttggtg gatttcttcc
     1801 gccgtcggtc gtggataagc cgattgagcg ccgctttggc gcgccgtatg tttcaccacg
     1861 tcccgagggg tctgctaaag ggaccgggcc taagattcca tcaagggcga cggtccccgg
     1921 gagcgcaacc ggagatagca tcaaaggcgg tccgagagga aatcgaggat cggctccacg
     1981 gccaggccgc tgagcatcga tgcagcggtc gggcacggag gcaggtcgct cgggacgctt
     2041 tctttcgtga gtatcccctc taagcagggg cactccgaga gggagccttg ccacgcttct
     2101 ccccactagt tcagtctcac ttgctaggct gcccgcgtct ggcccatcgg acggcggagg
     2161 gaagcgtggg gaagtttctg gatggtctgt tcgcggccct acgggtgggc ggagccgtcg
     2221 tcgctctggc gtactcggtc g
//
LOCUS       DASTZR010000010         6680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_575435, whole genome shotgun sequence.
ACCESSION   DASTZR010000010 DASTZR010000000
VERSION     DASTZR010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6680
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575435"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..609
                     /locus_tag="VFV20_00190"
     CDS             133..609
                     /locus_tag="VFV20_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286796.1"
                     /translation="MRAFVAAAVLAIVLGGAPVTAFADEGDTSDADVHADDPGITALH
                     DAITDMREASVALRAECPERSDAACRDAFKKVRESFKAARQKAIEAHHAFKQEQKSAR
                     EAARHKAKDSVKPHDKAKDQDKASDKDKGHDEASDRSKGHSPEPKGTPKAAPAPRT"
     gene            complement(661..1677)
                     /locus_tag="VFV20_00195"
     CDS             complement(661..1677)
                     /locus_tag="VFV20_00195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEU5286797.1"
                     /translation="MKLDAFLQGTPVETAGGISSGLEARGFHGMWLTETQVDPFPPLA
                     VAATRTTTLRLGTGIAVAFNRSPMLAAMDAWAIQRASGGRLDLGLGTQVKAHIERRFS
                     MPYDRPGARIREYVAALRHIWGAFQHEHRLAFKGDFYAFSLMSPFFDPGPIQWPRIPV
                     YLAAVNERMFRIAGEVADGVVGHPFSTPAYLREIGLPSLARGLARAKRERGAATLSCP
                     VFTLVESSRTFTADERYAREQIGFYASTPTYRAVLDHHGWGEVGEALSQLARTGKFDE
                     LPRLVTAEMFDTFVTRARTYAELGQRLRERYDGILDRVALYGDATALTTDEVRELSSG
                     LAGK"
     gene            1817..1975
                     /locus_tag="VFV20_00200"
     CDS             1817..1975
                     /locus_tag="VFV20_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286798.1"
                     /translation="MWELQGILLYKSIEHLGDANARHDADDDVLIPYAHLIRDPRVND
                     EEAKKLAA"
     gene            complement(1972..2481)
                     /locus_tag="VFV20_00205"
     CDS             complement(1972..2481)
                     /locus_tag="VFV20_00205"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007274452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEU5286799.1"
                     /translation="MPTPRRDRPHLPKGYISTAPKGMLAWSAVEKVVQRSPYLWIATT
                     DGDGRPHLVQQWGVWVDGTLYFEGSERTRWARNLARDQRLAFGVQTGSRAAYGEAVVD
                     VVRNVEPKLAAKIARQYASKYGRTFKYRPKAEQYAKGHVFRARPVKLIAFDVKRFTTS
                     AARFTFAPR"
     gene            2565..2735
                     /locus_tag="VFV20_00210"
     CDS             2565..2735
                     /locus_tag="VFV20_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286800.1"
                     /translation="MTTTTSGLVYCPNQKATIDVERCAACPWRSGGNATCVAEKRAEA
                     RDWRALMRILVP"
     gene            complement(2904..3614)
                     /locus_tag="VFV20_00215"
     CDS             complement(2904..3614)
                     /locus_tag="VFV20_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5286801.1"
                     /translation="MTTLSVENVESGYGEVQILWGTSLTLREGKLTTLVGSNGAGKTT
                     LLRTVMGLIPAWKGRVSLDGRDITKLPPHDKAALGIVLVPEGRQLFSTMSILENLEMG
                     ATVGHARAEIRQSFDRVFTRFPRLAERRTQLAGTLSGGEQQMLAIARGLMAKPKILMF
                     DEPSLGLSPLFVMNLFEIIRELKKEGLTMLLVEQNVQMSLAVSDFAYVMARGKVEIEG
                     EARQVRQMPEVRKAYLGL"
     gene            complement(3611..4366)
                     /locus_tag="VFV20_00220"
     CDS             complement(3611..4366)
                     /locus_tag="VFV20_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5286802.1"
                     /translation="MSAILQLQEVTVRFGGLVACDAASFDVEEGSILGLIGPNGAGKT
                     TLFNAINGVYRRHSGRIVFGGQTISGLRPFEVARRGIARAHQVVRPLNDLSVIANVTV
                     GACFGRENLGLRDATKVAEETLASLGIADRAEMLAGKLNVAQKKRLELARALAARPRL
                     LLADEVLAGLNPQEVADMLGIFRHIKQQGVTVVMIEHLMHAVMNVSDKVVVLDYGKKI
                     AEGTPQQVQSDPKVIEAYLGDPKIAERFLQEGA"
     gene            complement(4363..5331)
                     /locus_tag="VFV20_00225"
     CDS             complement(4363..5331)
                     /locus_tag="VFV20_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU5286803.1"
                     /translation="MRRYRIPFLPLAIAFIAIVAAVPIATDNPSLREELFLMLMLITL
                     ASSINIIMGYTGYVSFGHIVFFGLGGYFSFYLMQVQGWHFLAALVAGAIPSAIIAVLI
                     GVPILRLRGAYFALATVGINEATRVFFNNFEPTGGAVGMFFNFSVYLQYGGAKAAGEL
                     AYYVMTIVAILTIATSFWIKRSKFGLALMAIREDQDVAMVVGIDPARAKVMAYVLSAV
                     FPALAGAAFFFKNGIIEPGHAFDLIRSIESLVMVVLGGIGTVAGPIVGAAVYEWLRGF
                     LITNSTLANFQLFLAGLLLLVVVLFVSAGLVGWLRNRFPVLRAYIP"
     gene            complement(5328..6191)
                     /locus_tag="VFV20_00230"
     CDS             complement(5328..6191)
                     /locus_tag="VFV20_00230"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU5286804.1"
                     /translation="MNLDALIPSAIDGLMLGFVYGLAALGLTLIFGVMKVINLSHGPV
                     IALGMYVVYLLFTNFGVNPYIGIVAALVFGLVFGVIEYLIAVDRVINEPELTTLLATF
                     SVNLMIIGIATSVFTTTPRAVDISLGSIGTGNITILGTHIVSVIVAILVAAALYALLF
                     RTRIGKSIRAVANNRAAAELMGIDSRRMLALSFGIGTALAMVSGGLLATLFSFTPLSG
                     GTYEVKSFVIVVLGGLGNPTGAILGGIVIGLLEGISTVFIPVSWVPVLEYVIFILILL
                     LRPSGLLGARA"
     gene            complement(6222..>6680)
                     /locus_tag="VFV20_00235"
     CDS             complement(6222..>6680)
                     /locus_tag="VFV20_00235"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5286805.1"
                     /translation="EPTFADIGEAAQFIVGPSQWEPQVKYSEAGAKTLNIPYYGTSVE
                     TFTTAYKAKFGAAPSYHSAGGYAAGLVLQRALEEAGGTDPAKVKDALNKMDLMTFFGH
                     IKFSNEAKTHGKQTAHDMVYVQWLKGSDGKLATQIVWPEEAKSASAQLRK"
BASE COUNT         1140 a         2228 c         2328 g          984 t
ORIGIN      
        1 ctcgctgtgt gtcacacgac acggtcgtgg acgccgaacg aacgggggag ccgaacgggg
       61 gagggaagcc cctaccgctg ccgcgagtct tctcgttcgt acaggcatga ccggtaaggg
      121 tggtgggtgg cgatgagggc atttgtcgcg gccgcggtcc tcgcgatcgt ccttggcggg
      181 gcgccggtca cggcgttcgc cgacgagggc gacacgtccg atgcggacgt tcacgcggac
      241 gacccgggca tcacggcgct gcacgatgcg atcacggaca tgcgcgaggc gagcgtcgcc
      301 ctgcgcgcgg agtgccccga gcgctctgac gccgcgtgcc gcgacgcctt caagaaagtg
      361 cgcgagtcgt tcaaggctgc gcgtcagaag gcgatcgagg cgcaccacgc gttcaagcaa
      421 gagcagaaga gcgcgcgcga ggccgcgcgg cacaaggcga aggacagcgt gaagccgcac
      481 gacaaggcga aggaccagga caaggccagc gacaaggaca agggccacga cgaggccagc
      541 gacaggtcga agggccacag ccccgagccg aagggaacgc cgaaggcggc gcccgcgccc
      601 cggacctagc tcaccagccc ctcccgacga agaagcggct cccctcccgg ggccgctttt
      661 tcatttcccg gcgagtccgg acgagagctc gcgcacctcg tcggtcgtga gtgcggtggc
      721 gtcgccgtac agcgcgacgc gatcaaggat cccgtcgtac cgctcgcgca gacgctgccc
      781 gagctcggcg taggtgcgcg cgcgtgtcac gaaggtgtcg aacatctcgg cggtgaccag
      841 gcgcggcagc tcgtcgaact tcccggtgcg cgccagctgc gagagcgctt cgcccacctc
      901 accccagccg tggtggtcga gcaccgcgcg atacgtcggg gtcgaggcgt agaagccgat
      961 ctgctcgcgc gcgtagcgct cgtcggcggt gaacgtgcgc gacgactcga cgagcgtgaa
     1021 caccgggcac gacagcgtcg ccgcgccgcg ctcgcgcttc gcccgcgcga ggccgcgcgc
     1081 cagcgatggc agcccgatct cccgcaggta ggccggcgtc gagaacgggt ggccgacgac
     1141 gccgtcggcg acctcgccgg cgatccggaa catgcgctcg ttcaccgcgg ccaggtacac
     1201 cgggatgcgc ggccactgga tcgggccggg atcgaagaac ggcgacatga gcgagaacgc
     1261 gtagaagtcg cccttgaacg cgagccggtg ctcgtgctgg aacgcgcccc agatgtggcg
     1321 cagcgccgcg acgtactcgc ggatccgcgc gccgggacgg tcgtacggca tcgagaagcg
     1381 gcgctcgatg tgcgccttca cctgcgtccc gagtccgagg tcgaggcggc caccgctcgc
     1441 gcgctggatc gcccaggcgt ccatcgccgc gagcatcggg ctgcggttga aggcgaccgc
     1501 gatgccggtg ccgagccgca gcgtcgtggt ccgcgtggcc gcgaccgcga gcggcgggaa
     1561 cggatcgacc tgcgtctcgg tgagccacat cccgtggaag ccgcgcgcct cgaggccgct
     1621 cgagatgccg ccggcggtct cgaccggcgt tccctggagg aaggcgtcga gcttcacgag
     1681 agcgaaggct agcgatatgt gacgcaggtc acatccggca gcgtgtcctt cgtcacagac
     1741 gccgccaaat cggatgccgc ggcgtcgcgg gctcgttgga atgaaggaga actgaaggaa
     1801 gaaggagtgc gctccgatgt gggaactgca ggggatcctg ctctacaagt ccatcgagca
     1861 cctcggcgac gcgaacgcgc gtcatgacgc cgacgacgat gtgctcatcc cgtacgccca
     1921 cctcatccgc gacccgcggg tcaacgacga ggaagcgaag aagctcgccg cctagcgcgg
     1981 cgcgaacgtg aaccgcgccg ccgacgtggt gaagcgcttc acgtcgaagg cgatgagctt
     2041 caccgggcgg gcgcgaaaga catggccctt cgcgtactgc tcggcctttg gccggtactt
     2101 gaacgtgcgg ccgtacttcg acgcgtactg ccgcgcgatc ttcgccgcga gcttcggctc
     2161 gacgttccgg acgacgtcga ccacggcctc gccgtaggcg gcgcggctcc cggtctgcac
     2221 gccgaaggcg agccgctggt cgcgggcgag attgcgcgcc cagcgcgtcc gctcgctgcc
     2281 ctcgaagtac agggtgccgt ccacccagac gccccactgc tgtacgagat gcggacgtcc
     2341 gtccccgtcg gtcgtcgcga tccagaggta cggcgaccgt tgcacgacct tctcgacagc
     2401 cgaccacgca agcatcccct tcggagccgt cgagatgtag cccttcggca ggtggggacg
     2461 gtcgcggcgg ggtgtcggca cgacaggaat cgtaggacct cgaggccggc cgctgggtcg
     2521 aatggcccta tctccgcgtt cgtcgcgctc gtgtacttga acgcatgacc accaccacat
     2581 ccggcctggt ctactgcccg aaccagaagg cgacgatcga cgtcgagcgc tgcgcggcgt
     2641 gcccgtggcg cagcggcggc aacgcgacct gcgtggccga gaagcgcgcc gaggcgcgcg
     2701 actggcgcgc cctcatgcgc atcctcgttc cgtaaacgtc gcgacacttg cgacacaggc
     2761 gcccgctcgt tcgtcctctg tgatgaaagg ggtgcgcgcg atgtggctcc agggtctggt
     2821 cctgctctac gagtacctcg gacgcctgcg cgtccggaag accgcccgga tctagcaccg
     2881 gcggccccgg ccggcgcgct cggctacagc ccgaggtagg ccttccgcac ctccggcatc
     2941 tgccggacct gccgcgcctc cccctcgatc tcgaccttcc cgcgcgccat gacgtaggcg
     3001 aagtcggaga cggcgaggct catctgcacg ttctgctcga cgaggagcat ggtcagcccc
     3061 tccttcttga gctcccggat gatctcgaag aggttcatca cgaagagcgg cgagaggcca
     3121 aggctcggct cgtcgaacat caggatcttc ggcttcgcca tgaggccccg cgcgatggcg
     3181 agcatctgct gctcgccgcc cgagagcgtc ccggcgagct gggtgcgccg ctcagccagg
     3241 cgcgggaagc gcgtgaagac gcggtcgaag ctctggcgga tctccgcccg ggcatggccg
     3301 acggtcgctc ccatctcgag gttctcgagg atgctcatgg tcgagaagag ctgccggccc
     3361 tcggggacca gcacgatccc cagcgcggcc ttgtcgtgcg gcggcagctt cgtgatgtcc
     3421 ctgccgtcga gcgagaccct gcccttccac gccgggatga ggcccatgac cgtgcgcaag
     3481 agcgtcgtct tgccggcgcc gttgctgccg acgagcgtcg tgagcttgcc ctcgcgcagc
     3541 gtgagcgagg tgccccagag gatctggacc tcgccgtagc cggactcgac gttctcgacg
     3601 gagagggtgg tcacgcgccc tcctggagga agcgctccgc gatcttcggg tcacccaggt
     3661 acgcctcgat gaccttcggg tcgctctgga cctgctgcgg cgtgccctcg gcgatcttct
     3721 tcccgtagtc gagcacgacg accttgtcgg acacgttcat caccgcgtgc atgaggtgct
     3781 cgatcatcac gaccgtcacg ccctgctgct tgatgtgccg gaagatgccg agcatgtcgg
     3841 cgacctcctg cgggttcagc ccggcgagga cctcgtcggc gagcagaagc cgcgggcgcg
     3901 ctgccagcgc gcgcgcgagc tcgagccgct tcttctgcgc gacgttcagc ttgcccgcga
     3961 gcatctccgc gcggtcggcg atgcccagcg acgcgagcgt ctcctccgcg accttcgtcg
     4021 cgtcgcgaag accgaggttc tcgcggccga agcacgcgcc gacggtcacg ttcgcgatca
     4081 ccgagaggtc gttgagcggc cgcaccacct ggtgggcgcg cgcgatgccg cgccgcgcga
     4141 cctcgaacgg gcggagcccg ctgatggtct ggccgccgaa cacgatcctg ccgctgtggc
     4201 gccggtacac gccgttgatg gcgttgaaga gcgtcgtctt ccccgcgccg ttcggaccga
     4261 tgagcccgag gatcgagccc tcttcgacgt cgaacgacgc ggcgtcgcat gcgacgagcc
     4321 cgccgaaccg gaccgtgacc tcctggagct gcaggatcgc gctcatggga tgtacgcccg
     4381 cagcactggg aagcggttgc gcagccagcc caccaggccc gcgctgacga agagcacgac
     4441 gacgaggagc agcaggcccg cgaggaagag ctggaagttc gcgagcgtcg agttcgtgat
     4501 gaggaagccg cgcagccact cgtagaccgc cgcacccacg atcgggccgg cgacggtgcc
     4561 gatgccgccc agcacgacca tgacgagcga ctcgatcgag cggatgaggt cgaacgcgtg
     4621 gccgggctcg atgatgccgt tcttgaagaa gaacgcggcg ccggcgaggg cggggaacac
     4681 cgcggacagg acgtaggcca tgaccttcgc gcgcgcgggg tcgatgccca ccaccatggc
     4741 aacgtcctgg tcctcgcgga tggccatgag ggcgaggccg aacttggagc gcttgatcca
     4801 gaacgacgtc gcgatggtca ggatggcgac gatggtcatc acgtagtagg cgagctcgcc
     4861 cgcggccttc gcgccgccgt actgcaggta caccgagaag ttgaagaaca tgcccaccgc
     4921 gccgcccgtc ggctcgaagt tgttgaagaa cacgcgcgtc gcctcgttga tgccgaccgt
     4981 cgcgagcgcg aagtacgcgc cgcgcagccg caggatcggc acgccgatga ggaccgcgat
     5041 gatcgcggac gggatcgcgc cggcgacgag cgcggcgagg aagtgccaac cctgcacctg
     5101 catgagatag aacgagaagt acccgcccag gccgaagaac acgatgtgtc cgaacgacac
     5161 gtagcccgtg tatcccatga tgatgttgat gctcgacgcg agcgtgatga gcatcagcat
     5221 gaggaagagc tcctcgcgca gcgacggatt gtcggtggcg atcggcaccg ccgcgacgat
     5281 cgcgatgaac gcgatcgcca ggggcaggaa cgggatgcgg taacgcctca tgcgcgcgcc
     5341 ccgagcaggc ccgacgggcg caacagcagg atgaggatga agatcacgta ctcgagcacc
     5401 ggcacccacg agaccgggat gaacacggtc gagatgccct cgaggagtcc gatgacgatg
     5461 ccgccgagga tcgcgcccgt cgggttgccg agcccgccga gcacgacgat gacgaaggac
     5521 ttcacctcgt acgtgccgcc cgagagcggc gtgaacgaga agagcgtcgc caggagaccg
     5581 ccgctgacca tcgcgagggc ggtgccgatg ccgaaggaga gcgcgagcat ccggcgcgag
     5641 tcgatgccca tgagctccgc ggcggcgcgg ttgttcgcga cggcccggat ggacttgccg
     5701 atccgcgtgc ggaagaggag cgcgtagagc gccgctgcga caaggatcgc gacgatgacc
     5761 gacacgatgt gcgtcccgag gatcgtgatg ttcccggtgc cgatggagcc cagggagatg
     5821 tcgaccgcgc gcggcgtcgt cgtgaagacg ctcgtcgcga tgccgatgat catgaggttc
     5881 accgagaacg tcgcgaggag cgtcgtcagc tccggctcgt tgatgacgcg atcgaccgcg
     5941 atgaggtact cgatgacgcc gaagacgagg ccgaagacga gcgcggcgac gatgccgatg
     6001 tacggattga cgccgaagtt cgtgaacagc aggtacacga catacatgcc gagcgcgatg
     6061 accgggccgt gactcaggtt gatgaccttc atcacgccga agatgagcgt gaggccgagg
     6121 gccgccaggc cgtagacgaa gccgagcatg agtccatcga tcgccgacgg gatgagggcg
     6181 tcgaggttca tgctcggctc gtacggccta ttcggttttc gttacttgcg cagctgcgcg
     6241 cttgcggact tggcctcctc cggccagacg atctgcgtgg cgagcttgcc atccgagccc
     6301 ttgagccact gcacgtagac catgtcgtgc gcggtctgct tgccgtgggt cttggcctcg
     6361 ttcgagaact tgatgtgccc gaagaacgtc atgaggtcca tcttgttgag cgcgtccttg
     6421 accttcgccg ggtcggtccc gccggcctct tccagcgccc gctggaggac gaggcccgcg
     6481 gcgtagccgc ccgccgagtg gtacgacggc gccgcgccga acttcgcctt gtacgccgtg
     6541 gtgaacgtct cgacgctcgt gccgtagtac gggatgttca gggtcttcgc gcccgcctcc
     6601 gagtacttca cctgcggctc ccactgcgag gggccgacga tgaactgcgc cgcctcgccg
     6661 atgtcggcga acgtgggctc
//
LOCUS       DASTZR010000011         3305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_862440, whole genome shotgun sequence.
ACCESSION   DASTZR010000011 DASTZR010000000
VERSION     DASTZR010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862440"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="VFV20_00240"
     CDS             complement(<1..1056)
                     /locus_tag="VFV20_00240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEU5286806.1"
                     /translation="MTGRRWWWALGCAVAVLALLSAVQIARGVPFGYDFEAYWSVGRN
                     LAAGAPLYPAPDEALGQRGQFKYLPIVAVPFLLLAALPLQTATLVWFGIEIAIAAAVG
                     VVLLGELPRDARPWAAAGYVLFLPLALEVTIGNLNLASVALMLLAWRLRDRPARAAIP
                     LALGVGLKLLPAAMLLFYLAAGRWRVVALGALAGIAAILLTAPFLAGPLADYIVLFPR
                     LGDTTWVRTVVERDEPAILADILWSTAFQRAMSLATVVGVVLLGWMSRRSRDETQLHN
                     VALAISPYLAPFAFVWTTFLVMSLPLFASTLARVLALRGPGRALGIAGIALCLLLMDL
                     VRLHDLVPIAAHFAGVLL"
     gene            complement(1053..1274)
                     /locus_tag="VFV20_00245"
     CDS             complement(1053..1274)
                     /locus_tag="VFV20_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011518887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEU5286807.1"
                     /translation="MSPIRIQPNGPYVVTGPITIEDADGHVITVAEGHTVKLCRCGHS
                     GTKPYCDGTHRRVDFVSRPLFEPEPVERT"
     gene            complement(1271..1783)
                     /locus_tag="VFV20_00250"
     CDS             complement(1271..1783)
                     /locus_tag="VFV20_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyuridine 5'-triphosphate
                     nucleotidohydrolase"
                     /protein_id="HEU5286808.1"
                     /translation="MSVLSREELRAALAADPPLVEGIDQAVQLQPSGIDLRVERVQRL
                     TSPGLLGAADNVREPAAREDVAPDVDGWWALPQGAYVITYREKVNIPLDLVGFSWPRS
                     SLPRSGVAIHGAVWDPGYSGRGEGMLSVNNARGFRLQRGARVLQIVFFRLASAAGEGY
                     RGRYHGENPT"
     gene            1826..2308
                     /locus_tag="VFV20_00255"
     CDS             1826..2308
                     /locus_tag="VFV20_00255"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEU5286809.1"
                     /translation="MVAGKPVSGSRTVMSRTMLPSDANPYGNVHGGEVMKLIDACAGA
                     AATRHARSRVVTAFIDELSFLAPVYVGDLVTAKASVNHVGRTSMEVGVRVEAENLLTG
                     KVVHVSSAHLVFVSIDEKGQPEPLPPLIAETDDERRRMQAAEQRRRQRLARSGPVQAT
                     "
     gene            2337..>3305
                     /locus_tag="VFV20_00260"
     CDS             2337..>3305
                     /locus_tag="VFV20_00260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEU5286810.1"
                     /translation="MMRISRAFVPLVGLILVTACAAPRQSAATPTATAAATGTTAASA
                     TPSFAGKTLNIVTGGTGGVYIVYGAGLADLLNKKLGTAASPQSTPASVDNMKLIRDGK
                     ADLAFVLSDTAYDAVQGKGAFAPPQTKADAKVLAVLYNNFTHVVVRDDSPIKTVADVK
                     GKRVNTGAAGSGTETIANRTLEAYGLDPAKDIQRERTGVADAAAQLKDNRIDAFFWSG
                     GLPTAAVTDLTTTTKIRILDQADGVKKMAEKYGPFYFNAKIPKGTYGIAADVSVSGVA
                     NVLVVPSSFDAALAKAILKTMFDSSTELGQIHAEGKNLKLETAVEGS"
BASE COUNT          575 a         1153 c         1173 g          404 t
ORIGIN      
        1 cagcagcacg cccgcgaaat gagccgcgat cggcacgaga tcgtgcagcc gcaccagatc
       61 catcagcagc aggcacagcg cgatacccgc gataccgagc gcccgcccgg gtccgcgtag
      121 cgcgagcacg cgggcgaggg tgctcgcgaa aagcggcaac gacatgacca ggaacgtcgt
      181 ccacacgaac gcgaacggcg cgagataggg cgagatggcg agcgccacgt tgtggagctg
      241 cgtctcatcg cgcgagcgac ggctcatcca tccgaggagc acgacgccca ccacggtcgc
      301 gaggctcatc gcgcgttgga acgcggtgga ccacaggatg tccgcgagga tcgcgggctc
      361 gtcgcgctcg acgaccgtgc gcacccaggt cgtatcgccg agccgcggga acagcacgat
      421 gtagtccgcg agcggcccgg ccaggaacgg cgcggtcagc aggatcgcgg cgatgccggc
      481 gagcgccccc agcgccacga cgcgccatcg gccggcggcg aggtagaaga ggagcatcgc
      541 ggcgggcagc agcttgaggc cgaccccgag cgcgagcggg atcgccgcgc gggccgggcg
      601 gtcgcggagg cgccaggcca gcagcatgag cgcgacgctc gcgaggttga gattgccgat
      661 ggtcacctcg agcgcgagcg ggaggaacag cacgtacccg gcggcggccc agggccgcgc
      721 gtcccgcggc agctcgccga gcagcacgac gccgacggcg gccgcgatcg cgatttcgat
      781 gccgaaccag acgagcgtcg ccgtctgcag cggcagcgcg gcgagcagga ggaacggcac
      841 ggcgacgatc ggcaggtact tgaactggcc gcgctgaccg agcgcctcgt cgggtgccgg
      901 atacagcggc gcgcccgcgg cgaggttcct tccgacggac cagtacgcct cgaagtcgta
      961 accgaacggg acgccgcgcg cgatctggac cgccgagagg agcgcgagca cggccacggc
     1021 gcagccgagc gcccaccacc agcgtcgccc ggtcacgtgc gctcgaccgg ctccggctcg
     1081 aacagtggcc gggagacgaa gtcgacgcgc cggtgcgtcc cgtcgcagta cggcttcgtg
     1141 ccggaatggc cgcagcggca cagcttcacc gtgtggccct cggcgacggt gatgacgtgg
     1201 ccgtccgcgt cctcgatcgt gatggggccg gtcacgacgt acggtccgtt gggctggatg
     1261 cggatggggc tcatgtcgga ttctcgccgt ggtagcggcc gcggtatccc tcgccggccg
     1321 cggaggcgag acggaagaag acgatctgca gcacgcgcgc accgcgctgc aagcggaagc
     1381 cgcgcgcgtt gttgaccgac agcatgccct cgccgcgccc ggagtagccc ggatcccaga
     1441 ccgcgccgtg gatcgcgaca cccgagcgcg gcaggctcga gcgcggccag gagaatccga
     1501 cgaggtcgag cgggatgttg accttctcgc gataggtgat cacgtaggcg ccctgcggca
     1561 gggcccacca gccatcgacg tccggcgcga cgtcctcacg ggcggccggc tcgcggacgt
     1621 tgtccgcggc gccgagcagc ccgggcgacg tcagacgctg cacgcgctcg acgcgcagat
     1681 cgatcccgct cggctggagc tgcaccgcct ggtcgatgcc ctccaccagt ggcgggtccg
     1741 cggcgagcgc ggcccgcagc tcctcgcgcg acagcaccga catgccgaaa gtatgcgtgc
     1801 ccaccgcact cgataggatt cgggcgtggt cgccgggaaa cccgtctcgg gttcgcgcac
     1861 cgtgatgtcg cgcacgatgc ttccctcgga cgccaatccg tacggcaacg tccacggcgg
     1921 cgaggtgatg aagctcatcg acgcatgcgc gggggcggcc gcgacgcggc acgcgcgcag
     1981 ccgcgtcgtg acggcgttca tcgacgagct gtcgttcctc gcgccggtgt acgtcggcga
     2041 tctcgtcacc gcgaaggcct cggtgaacca cgtgggccgg acgagcatgg aggtgggcgt
     2101 gcgcgtggag gctgagaacc tgctgacggg caaggtcgtc cacgtgtcct cggctcacct
     2161 ggtgttcgtt tcgatcgacg aaaaaggcca gcccgagccc ctaccgccgc tcatcgccga
     2221 gaccgacgat gagcgccggc gcatgcaggc ggccgagcag cgccgcaggc aacgtctcgc
     2281 gcgctcggga cccgtccagg cgacgtagac tcggctccat tcggagggtg tgacagatga
     2341 tgcggatctc acgcgctttc gttcctctgg tcggattgat cctcgtcacc gcctgcgccg
     2401 cgccgcgcca atcggccgcg acgccgacgg cgaccgcggc cgccaccggc accacggccg
     2461 catccgcgac cccgagcttc gcgggcaaga cgctgaacat cgtgacgggc ggcaccggcg
     2521 gcgtgtacat cgtgtacggc gccggcctcg ccgacctgct caacaagaag ctcggcacgg
     2581 cggcgagccc gcagtccact cccgcgtcgg tcgacaacat gaagctcatc cgtgacggca
     2641 aggccgacct cgcattcgtg ctctccgaca ccgcgtacga cgcggtgcag ggcaagggcg
     2701 cgttcgcgcc gccgcagacg aaggcggacg ccaaggtcct cgcggtgctc tacaacaact
     2761 tcacgcacgt ggtcgtgcgc gatgacagcc ccatcaagac cgtcgccgac gtcaagggca
     2821 agcgagtgaa caccggggcg gccggctcgg gcaccgagac gatcgcgaac cgcacgctcg
     2881 aggcgtacgg actcgacccg gcgaaggaca tccagcgcga gcgcaccggc gtcgccgacg
     2941 cggccgcgca gctgaaggac aatcgcatcg acgccttctt ctggagcggc ggcctgccga
     3001 ccgcggcagt gacggacctc acgacgacga cgaagatccg catcctcgac caggcggacg
     3061 gcgtcaagaa gatggccgag aagtacggtc ccttctactt caacgcgaag atcccgaagg
     3121 gcacctacgg catcgccgcc gacgtcagcg tgtccggcgt ggcgaacgtg ctcgtcgtgc
     3181 cgtcgagctt cgacgccgcg ctcgccaagg cgatcctgaa gacgatgttc gacagcagca
     3241 ccgagctcgg gcagatccac gccgagggca agaacctgaa gctcgagacc gcggtggagg
     3301 gctcg
//
LOCUS       DASTZR010000012         4964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_941065, whole genome shotgun sequence.
ACCESSION   DASTZR010000012 DASTZR010000000
VERSION     DASTZR010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941065"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VFV20_00265"
     CDS             complement(<1..809)
                     /locus_tag="VFV20_00265"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5286811.1"
                     /translation="MRPSDLLGLALANVGRARVRSILTILGVAIGTALVVLLIALASG
                     AEENVKRSIFSIGDLRLVSVQPFQPGSTGLTAVGRAITDDHVTKFRTIPHVSGVYRQF
                     DAPLGTLVEAGDDAAIRPQGIESDAPVDRGDLLAGRHLGPNERAVAVIPGNIARLLAP
                     TVDAAVGKKVTLRLGGAVKIGQTRIAGSGTPRDYAVTVIGVFDERNTSQTSVRIALDD
                     AVLAAGENRGLSADEMRRTTGYSGVSLEAEDSKYVGEIVSAVQEQGFSAFSL"
     gene            complement(806..1489)
                     /locus_tag="VFV20_00270"
     CDS             complement(806..1489)
                     /locus_tag="VFV20_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5286812.1"
                     /translation="MAQAPLLIVQATGLERTYQSGESRVAALAGLDLEVRRGEFLAVM
                     GPSGSGKSTLLNILAGLERPTAGRIAVDGIDLGSLEEAALARYRRERIGIVFQAFNLL
                     PRYRVVENVAFPLLFAGVPRAERLRRANEILARLGMAARASHRPNQLSGGEMQRTAIA
                     RALVTEPSLLLADEPTGNLDTANGEALLAILSELHERGQTIVLVTHEDSVASRAERIV
                     RMRDGRIAA"
     gene            complement(1468..2193)
                     /locus_tag="VFV20_00275"
     CDS             complement(1468..2193)
                     /locus_tag="VFV20_00275"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5286813.1"
                     /translation="MPALVLLHAFPLDRTMWDRECRTLAGAASPIIAPSFPGFGRSEL
                     PTHQPSVDDYADAVIAVMDAGNVERAAIAGCSMGGYVAFAVWRRHRARVAGLALIDTK
                     AEADTPEAAQNRIRLAETIREHGVEALLKTPPKWLRDGSPKWPALKDLIRAQDKEAVA
                     QASVAMAGRPDSTPDLATIDVPTAILVGEADAITPLAASRAMNEAIPHSTLSVLPDAG
                     HLSNLEAPEAFDRAMRAWLKRLS"
     gene            2223..2579
                     /locus_tag="VFV20_00280"
     CDS             2223..2579
                     /locus_tag="VFV20_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5286814.1"
                     /translation="MRVVHIGKAGRQPARNPIFKGEVEDQTAVGDDTSRELRLSEIHF
                     RNGAVNKWHVHSCDQILIVTEGEGIVADEREQRVVGAGDVAFIPANTRHWHGAKPGKD
                     MTHWSILGNGKTTIAE"
     gene            2611..3369
                     /locus_tag="VFV20_00285"
     CDS             2611..3369
                     /locus_tag="VFV20_00285"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEU5286815.1"
                     /translation="MSAVAGIAVHEADVRPHPLLVSIFLHLAPGAAVLGAYLGLLPGV
                     RAAGLPSNVALLVAFLAIGAPLLVGLLARAGRDRDGRFPAVRYREGLAWPRSAWLVAG
                     LSLWLVAVSSVLGPLEQTLQLKGMFAWIPADLRLDDLVTAGPTGLAVVTALSLVANVV
                     VPATEELYFRGYLLPRLPLGGRAAVLVNTLLFSLYHFWLPWQFASRVMGLLPMVASVR
                     RSRSVAVGIAVHVIVNSTGTLALLAMLLANSARQ"
     gene            complement(3363..4232)
                     /locus_tag="VFV20_00290"
     CDS             complement(3363..4232)
                     /locus_tag="VFV20_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEU5286816.1"
                     /translation="MNTIKTVGLLTVLTLLLIAAARIFFGPSAVPIALVLAAVMNFGS
                     WWFSDKIALRFAGAHPVSEADAPDLYRIAGRVAQIANIPMPRVYVIDQAAPNAFATGR
                     DPQHAVVAATRGIMEITNERELTAVLAHEVGHVKNRDTLVMAVVATIAGAISMLAQMA
                     QWSLWMGGGRRDDRDSGSLGMIGMIAGIILLPLAAMLVQLAVSRSREYGADDQSAALT
                     HDPLALASALRKLDAYGKKVPLPVNPAVAPLFIVQPLLPGGMSGLFSTHPPIEQRIAR
                     LQQMAGTELPQTY"
     gene            4306..4680
                     /locus_tag="VFV20_00295"
     CDS             4306..4680
                     /locus_tag="VFV20_00295"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEU5286817.1"
                     /translation="MAGSAPKTVDAYIAAAPAAVRPKLRQLRATIRAAAPRATETISY
                     RMPYYAYHGRLIYFALQRTHVGLYMLGRAKTRYAKELAPYLSGASTARFPLDGRLPTG
                     LIRKVVAERVRENDERARSKKR"
     gene            complement(4677..>4964)
                     /locus_tag="VFV20_00300"
     CDS             complement(4677..>4964)
                     /locus_tag="VFV20_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5286818.1"
                     /translation="GGIVGGVGQAIGNETAIRVGTATRLLLPTDGMWRGAVWSLEPAT
                     VIAGVSAAGPASAANPFFAAAPPEPVYLAWTVAWIALGLAIAIWSFSRREV"
BASE COUNT          716 a         1756 c         1764 g          728 t
ORIGIN      
        1 agcgagaacg ccgagaagcc ctgctcctgc acggcgctca cgatctcgcc cacgtacttg
       61 gagtcctccg cctcgagcga cacgccgctg tagccggtgg tccggcgcat ctcgtcggcc
      121 gagaggccgc ggttctcgcc cgccgcgagg acggcatcgt cgagcgcgat ccgcaccgac
      181 gtctgcgagg tgttgcgctc gtcgaacacg ccgatgacgg tgacggcgta atcgcgcggt
      241 gtgccactcc cggcgatgcg cgtctggccg atcttcaccg cgccgccgag ccgcagcgtg
      301 accttcttgc cgaccgcggc gtcgacggtg ggcgcgagga gcctcgcgat gttcccgggg
      361 atcaccgcga cagcgcgctc gttcggtccg aggtggcgtc cggccagcag atcgccgcgg
      421 tccaccggcg cgtcggactc gatgccctgc gggcggatcg ccgcgtcgtc gccggcctcc
      481 accagcgtgc cgagcggcgc gtcgaactgg cggtacaccc ccgagacgtg cgggatggtg
      541 cggaacttcg tgacgtggtc gtctgtgatc gcgcgcccga ccgcggtgag cccggtcgag
      601 cccggctgga agggctgcac ggacacgagg cgcaggtcgc cgatggagaa gatcgagcgt
      661 ttcacgttct cctcggcgcc ggaagcgagc gcgatgagca ggacgacgag ggccgtcccg
      721 atcgcgacgc ccaggatcgt gaggatcgaa cggacgcgtg cgcgcccgac gttcgcgagc
      781 gcgaggccga gaaggtccga cggcctcacg ccgcgatcct gccgtcgcgc atgcggacga
      841 tccgctcggc gcgcgacgcg accgaatcct cgtgcgtgac gaggacgatc gtctgcccgc
      901 gctcatgaag ctcggacagg atcgcgagca gcgcctcgcc gttcgcggtg tcgaggttgc
      961 cggtgggctc gtccgcgagc aggagcgacg gctccgtcac gagcgcccga gcgatcgcgg
     1021 tgcgctgcat ctctccgccg gagagctggt tcggccgatg tgaggcgcgt gcggccatcc
     1081 cgagccgcgc gaggatctcg ttcgcgcgcc ggaggcgttc cgcgcgcggc acgcccgcga
     1141 agaggagcgg gaaggccacg ttctccacca cgcggtagcg cggcagcagg ttgaacgcct
     1201 ggaagacgat gccgatgcgc tcgcgccggt agcgcgccag cgcggcctcc tcgagcgagc
     1261 cgaggtcgat cccgtcgacc gcgatgcgtc ccgcggtcgg ccgctcgagg ccggcgagga
     1321 tgttgaggag cgtcgacttt cccgagcccg acgggcccat cacggccagg aactcgccgc
     1381 ggcgcacctc gaggtcgagg ccggcgagcg cggccacgcg cgactcgccg ctctggtagg
     1441 tgcgctcgag gcccgtggcc tggacgatca ggagaggcgc ttgagccacg cgcgcatcgc
     1501 ccggtcgaac gcctccggcg cctcgaggtt cgagagatgt ccggcgtccg ggagcaccga
     1561 gagggtcgag tgggggatcg cctcgttcat cgcgcgcgac gcagcgagcg gggtgatcgc
     1621 gtcggcctcg ccgacgagga tcgcggtcgg cacgtcgatc gtcgcgagat cgggcgtcga
     1681 gtccggccgt ccggccatcg cgacgctcgc ctgcgcgacg gcctccttgt cctgcgcgcg
     1741 gatgaggtcc ttcagggcgg gccacttggg cgatccgtcg cgcagccact tgggcggcgt
     1801 cttcagcagc gcctcgacgc cgtgctcgcg gatggtctcc gcgagacgga tgcggttctg
     1861 cgccgcttcg ggcgtgtccg cttcggcctt cgtgtcgatg agcgctaggc cggcgacacg
     1921 cgcgcgatgg cgccgccaaa cggcgaaggc gacgtacccg cccatcgaac agcccgcgat
     1981 cgccgcgcgc tccacgttgc cggcgtccat gacggcgatc accgcgtcgg cgtagtcgtc
     2041 caccgacggc tggtgcgtgg gcagctcgct cctaccgaat cccgggaacg acggcgcgat
     2101 gatcggcgag gccgcgcccg cgagcgtgcg gcactcgcgg tcccacatcg tgcggtcgag
     2161 cgggaaggcg tggagcagga cgagcgcggg cataccgcct cagtgtgcga cacaatcgtt
     2221 ccatgcgcgt ggtccacatc gggaaggcgg gccggcagcc cgcacggaac ccgatcttca
     2281 agggcgaggt cgaggaccag acggcggtcg gcgacgacac cagccgcgag ctccgtcttt
     2341 ctgagatcca cttcaggaac ggcgccgtca acaagtggca cgtccactcc tgcgaccaga
     2401 tcctcatcgt gaccgagggc gagggcatcg tcgcggacga acgggagcag cgcgtcgtcg
     2461 gggcgggcga cgtcgccttc atccccgcga acacgcgcca ctggcacggc gcaaagccgg
     2521 ggaaggacat gacccactgg tccatcctcg ggaacggcaa gacgacgatc gcggagtgat
     2581 gtcagcgcca ggtcgatgga ctccgcgcac gtgagtgcgg tcgccggcat tgcggtgcac
     2641 gaggcggacg tcaggccgca tccgctcctc gtctcgatct tcctccacct cgcacccggt
     2701 gccgccgtgc tcggcgcgta tctcgggcta ctgcccggtg tgcgcgcggc gggactcccc
     2761 tccaacgtcg cactgcttgt cgcgttcctc gccatcggtg caccgctgct cgtcgggctc
     2821 ctggcgcgcg cgggacgcga tcgggacggt cggttcccgg cggtgcggta ccgcgagggg
     2881 ctcgcgtggc cgcgctcggc atggctcgtc gcgggcctct cgctctggct ggtagcggtc
     2941 tcgtcggtcc tcggtccgct tgagcagacg ctccagctca aagggatgtt cgcgtggata
     3001 cccgcggatc ttcgcctcga cgacctcgtg accgccggcc cgactggcct tgccgtcgtg
     3061 acggcgctct cactcgtcgc gaacgtcgtg gtgcccgcca cagaggagct gtactttcgc
     3121 ggctacctgc tgccgcgcct cccgttggga ggccgcgcgg cggtcctcgt gaacacgctc
     3181 ctcttttcgc tgtaccactt ctggctaccg tggcagttcg cgagccgcgt catggggctg
     3241 cttccgatgg tcgcgagcgt gcgccgcagt cgaagcgtcg cggtcggcat cgccgtccac
     3301 gtcatcgtca actcgacagg cacgctcgct ctcctcgcga tgctgctcgc gaactcggcg
     3361 cgtcagtagg tctgcggcag ctccgtcccg gccatctgct gtaggcgcgc gatgcgctgc
     3421 tcgatgggcg ggtgcgtcga gaagagcccg ctcatcccgc ccggcaggag cggctgcacg
     3481 atgaagagcg gcgcgacggc cgggttcacc ggcagcggga ccttcttgcc gtatgcgtcg
     3541 agcttccgca gcgccgacgc cagcgcgagc gggtcgtgcg tcagggccgc gctctggtcg
     3601 tcggcgccgt actcgcgcga ccgcgagacc gcgagctgga ccagcatcgc ggcgagcggc
     3661 agcaggatga tgccggcgat catgccgatc atcccgagcg agccggagtc gcggtcgtcg
     3721 cggcgcccgc cacccatcca cagcgaccac tgcgccatct gcgcgagcat cgagatcgcg
     3781 cccgcgatcg tcgcgacgac ggccatgacg agcgtgtcgc ggttcttcac gtgcccgacc
     3841 tcgtgcgcca gcacggcggt gagctcgcgc tcgttcgtga tctccatgat cccgcgcgtc
     3901 gcggcgacaa cggcgtgctg cgggtcacga cccgtcgcga acgcgttcgg cgcggcctgg
     3961 tcgatgacgt acacacgcgg catcgggatg ttcgcgatct gcgcgacgcg ccccgcgatg
     4021 cggtagaggt ccggcgcgtc cgcctcgctc accgggtgcg cgcccgcgaa ccgcagggcg
     4081 atcttgtcgg agaaccacca ggagccgaag ttcatgacgg cggcgagcac gagcgcgatg
     4141 gggaccgccg acggtccgaa gaagatgcgc gccgccgcga tgagcagcag cgtgaggacg
     4201 gtcagcaggc cgacggtctt gatggtgttc atcatccgat tgtcacacct cctccgtgaa
     4261 ccatttgtga agcgcgccta agagcgccct acgatggcca tgaccatggc aggttccgcg
     4321 ccaaagacgg tcgacgcgta catcgccgcg gcccccgcgg cggtgcggcc caagctccgc
     4381 cagctccggg cgacgatcag ggcggcggcg ccgcgcgcga cggaaacgat cagctaccgg
     4441 atgccctact acgcctacca cggccggctg atctacttcg cgctgcagcg cacgcacgtc
     4501 ggcctctaca tgctcggacg cgccaagacg cggtacgcga aggagctcgc gccctatctc
     4561 tccggcgcgt cgacggcgcg ctttccgctg gacgggcgtc tgccgacggg gctcatccgg
     4621 aaggtcgtgg cggaacgcgt gagagagaac gacgagaggg cacgctcgaa gaagcgctag
     4681 acctcgcggc ggctgaagct ccagatcgcg atcgcgagac ccagcgcgat ccacgcgacg
     4741 gtccacgcga gatacaccgg ctccggcggc gccgccgcga agaacggatt cgccgcgctc
     4801 gccggccccg ccgcgctcac ccccgcgatg acggtggcgg gctcgaggct ccacacggcg
     4861 ccgcgccaca tgccgtcggt agggaggagc agccgcgtcg ctgtgcccac acggatcgcc
     4921 gtctcgttgc cgatcgcctg gccgacgccg ccgacgatcc ctcc
//
LOCUS       DASTZR010000013         2799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_418838, whole genome shotgun sequence.
ACCESSION   DASTZR010000013 DASTZR010000000
VERSION     DASTZR010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418838"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="VFV20_00305"
     CDS             complement(<1..1045)
                     /locus_tag="VFV20_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5286819.1"
                     /translation="MAAETHYAKSGSIHVAYQVTGDGPLDLIWSPGALSHVEMAWENP
                     YQRRFIERLSSFSRLVRFDKRGTGMSDPVSGAPTMDERIDDVRAVLDAVGSESAHLFG
                     VSEGGSMTMLFAATFPDRTRSLSLWGALPRWTIAPGYPWGDKAEDRERAASEIDAHGR
                     RPFTLTEDVKRWLGPVHEDPAFVDAWRRSRSASGSPAMVAALDRMNARIDVRDILPSI
                     RVPTLVMNRVGDPDASCDAARYTAGLIPGARFVEFPGYGHLFFDILDDVADTLEGFIT
                     GTRTAAPSDRVLATLLFVDIVGSTELLGRLGDATWRGVIERYYAIVRRLLVAYRGIEV
                     DTAGDGLLARFDGP"
     gene            1138..2757
                     /gene="ctaD"
                     /locus_tag="VFV20_00310"
     CDS             1138..2757
                     /gene="ctaD"
                     /locus_tag="VFV20_00310"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEU5286820.1"
                     /translation="MATAERTFPVAQRRYTGLLEWITTTDHKKIGILYVGTTFLFFLA
                     GGLLATGIRTQLATPDGTLLTAQQYNEAFTMHGTTMVFLFVVPVWTGFANYIIPLQIG
                     ARDMAFPRLNALSYWLLVAGAIVLYSSFIFGLPAAGWTGYVPLSNAQFSPGHGVDLWI
                     LGLTILGFSSIFGALNMIVTIFALRCPGLTFHRLTLFGWSVLVTSFLLVLAMPFLTVA
                     GVLLLFDRMAGTNFFAVSEGADPLLWQYLFWFFGHPEVYIMILPGFGVVSEVLPVFSR
                     KPIFGYKMIAYSSVAIGFLSFGVFVHHMFVAGIDPALQVFFMSSTMLIAVPTGVKIFS
                     WLGTIWGGALRYKTAMLFALGFIAVFTIGGISGVFLGSVPVDIQLSDTYYVIAHIHYV
                     LVGGALFTFFAACYYWIPKMSGRLLSERLGQLHFFIFFIGFNMAFFPMHQLGLEGMPR
                     RTYHYPQSPEWGFLNLVSTIGVYLIALAVTFFIWNFVSSVLMKNGRIADDDPWEADTL
                     EWATSSPPPPYNFVRIPVVHSARPLKEDLPH"
BASE COUNT          418 a          978 c          871 g          532 t
ORIGIN      
        1 ccgggccgtc gaaccgcgcg agaagtccat cgcccgcggt atcgacctcg attccgcgat
       61 aggccacgag cagccgccgc acgatcgcgt agtagcgctc gatgacgccg cgccaggtcg
      121 cgtcgcccag ccgtcccagc agctcggtcg agccgacgat gtccacgaag agcagtgtgg
      181 cgagcacgcg gtcggatggc gcggccgtcc gcgtcccagt gatgaaccct tcgagcgtgt
      241 cggccacgtc gtcgaggatg tcgaagaaga ggtggccgta acccgggaac tcgacgaacc
      301 tcgcacccgg aatgaggcca gccgtgtatc gggcagcgtc gcacgacgca tccgggtcac
      361 cgacgcggtt cattacgagc gtcggcacgc ggatcgacgg aaggatgtcg cgaacgtcga
      421 tgcgcgcgtt catgcggtcg agcgccgcga ccatcgccgg cgagcccgat gcgctgcgcg
      481 agcgacgcca cgcgtccacg aacgctggat cttcatgcac ggggccgagc cagcgcttca
      541 cgtcctccgt caaagtgaat ggccgtcggc cgtgtgcgtc gatctccgag gccgctctct
      601 cgcggtcttc ggccttgtct ccccacgggt aaccaggcgc gatcgtccaa cgcggcagcg
      661 cgccccagag cgacagcgag cgcgtgcgat cggggaacgt tgcagcgaag agcatggtca
      721 tggagccccc ctcggacacg ccgaacaggt gcgcgctctc gctacccacc gcatcgagca
      781 ccgcgcggac gtcgtcgatg cgctcgtcca tcgtcggtgc gccgctcacg ggatcggaca
      841 tgcccgttcc gcgcttgtcg aagcggacga ggcgcgagaa cgatgacagg cgctcgatga
      901 agcgcctctg gtaggggttc tcccaggcca tttcgacgtg cgagagcgcg ccgggcgacc
      961 agatcaggtc gagcgggccg tcacccgtga cctggtacgc gacgtggatg ctgccgctct
     1021 tcgcgtagtg cgtctcagcc gccatggacg cgccaaagtc taggctccac cgcccgcagc
     1081 gctgggtcgc tctcggtatt tgacatgggt gggatccgcc agtggaatgg acgtctcatg
     1141 gccaccgcag aacgcacgtt ccccgttgcc cagcgccgct acacgggcct gctcgagtgg
     1201 atcacgacca ccgaccacaa gaagatcggc atcctctacg tgggcacgac gttcctgttc
     1261 ttcctcgccg ggggtctgct cgcgaccggc atccgtacgc agctcgccac acccgacggc
     1321 acgctcctca ccgcccagca gtacaacgag gccttcacga tgcacggcac gacgatggtc
     1381 ttcctgttcg tcgtaccggt gtggaccggc ttcgcgaact acatcatccc gctgcagatc
     1441 ggcgcgcgtg acatggcgtt cccgcggctc aacgcgctct cgtactggct gctcgtcgcg
     1501 ggcgcgatcg tgctctattc gtcgttcatc ttcggtctgc cggccgcggg ctggacgggg
     1561 tacgtcccgc tctcgaacgc gcagttctcg ccgggccacg gcgtggacct ctggatcctc
     1621 ggcctcacga tcctcgggtt ctcgtcgatc ttcggcgccc tgaacatgat cgtcacgatc
     1681 ttcgcgctcc gctgccccgg cctgacgttc caccgtctca cgctgttcgg ctggagcgtg
     1741 ctcgtgacga gcttcctgct cgtgctcgcg atgccgttcc tcaccgtcgc gggcgtgctg
     1801 ctgctcttcg accgcatggc cgggacgaac ttcttcgcgg tgtcggaggg cgcggatccg
     1861 ctgctctggc agtacctgtt ctggttcttc ggacaccccg aggtctacat catgatcctg
     1921 cccggattcg gggtcgtctc cgaggtgctg ccggtgttct cgcgcaagcc gatcttcggc
     1981 tacaagatga tcgcgtactc gtcggtggcg atcggcttcc tctcgttcgg cgtgttcgtg
     2041 caccacatgt tcgtcgcggg catcgatccc gccttgcagg tcttcttcat gtcgtcgacg
     2101 atgctcatcg ccgtcccgac cggcgtgaag atcttctcgt ggctcggcac catctggggc
     2161 ggcgcgctcc gctacaagac ggcgatgctc ttcgcgctcg gcttcatcgc ggtgttcacc
     2221 atcggcggca tctccggcgt cttcctgggg tcggtgcccg tggacatcca gctgtcggac
     2281 acgtactacg tcatcgcgca catccactac gtgctcgtcg gcggcgcgct cttcacgttc
     2341 ttcgccgcct gctactactg gatccccaag atgagcggac gtctgctgtc cgagcggctc
     2401 ggccagctgc acttcttcat cttcttcatc ggcttcaaca tggcgttctt cccgatgcac
     2461 cagctcgggc tggagggcat gccacgccgc acgtaccact acccgcagtc gccggagtgg
     2521 ggcttcctca acctcgtctc gacgatcggc gtctatctca tcgcgctggc cgtcacgttc
     2581 ttcatctgga acttcgtgtc gagcgtgctc atgaagaacg gccgcatcgc ggacgacgat
     2641 ccgtgggagg ccgacacgct cgagtgggcg acgtcctcgc cgccgccgcc gtacaacttc
     2701 gtgcgcatcc ccgtcgtcca cagcgcgcgc ccgctgaagg aagatctccc gcattagccg
     2761 gcagcgccgc tcgtcgctcg gataggctga acacgtgac
//
LOCUS       DASTZR010000014         2683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_993324, whole genome shotgun sequence.
ACCESSION   DASTZR010000014 DASTZR010000000
VERSION     DASTZR010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993324"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VFV20_00315"
     CDS             <1..394
                     /locus_tag="VFV20_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286821.1"
                     /translation="QAVAFARSISPTVKAVHIATTAESADEFRDRWRRWSSDIPLDVI
                     ESPYRSLLQPLLRYIERIDERDDRPITVVLAEFVPRNWWEWILHSQTALRLKLALLFR
                     PNTIVIDVPYHFADTGHAKRRDKDRPDS"
     gene            432..860
                     /locus_tag="VFV20_00320"
     CDS             432..860
                     /locus_tag="VFV20_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5286822.1"
                     /translation="MQLRRIVVPLAGTSVDPDVIRVAATLAKPTKAAIIAIHVIEVRW
                     NLPLDAVLEADTERGELLLDEAIKVAEQAGTTIETELLQAREAAAAIIDTAREQNADL
                     IVLGMPFRKRLGRVYIGRTVQQVYVGAPCAVLAYRQEEVR"
     gene            857..1324
                     /locus_tag="VFV20_00325"
     CDS             857..1324
                     /locus_tag="VFV20_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEU5286823.1"
                     /translation="MKIVIIGCGRVGAFLAGLLDAGGHEVTVVDLERSAFGHLPADFH
                     GTTLLGNGTDMEVLRQAGIDRADALLTLTQGDNRNLMSAQVAKQIFGVKQVIAKVNDP
                     IRANTYRTHGIATFSRTSILGTLLEAMLMGDPEVGKVLVERARGHEARLAGQS"
     gene            1325..1984
                     /locus_tag="VFV20_00330"
     CDS             1325..1984
                     /locus_tag="VFV20_00330"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEU5286824.1"
                     /translation="MYFVIAGGGEVGFHLAKALLEAGHEIMLIESDRRRATFIQEQLG
                     SVVLNAPADEGRFQIEAGCQRADAVVAVTGEDPANLVICQLAKWKCNVPRVIARVNDP
                     KNEIVFKTLGIDETISSTRVLMNVIEQELPSPGGFLPLMPLTGSHLELIEAEVGPNTP
                     GAGNTIRSLGLPHGTAVGGIVRKGSVLHADEDVRLEVGDRLVILAPTKDEAEVRKALA
                     G"
     gene            complement(2012..>2683)
                     /locus_tag="VFV20_00335"
     CDS             complement(2012..>2683)
                     /locus_tag="VFV20_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEU5286825.1"
                     /translation="DLAVVRRDGDALRVIATAGRDIGGDRCTERVIDEAVAPRLGSRA
                     EWGPKRLRLPAFIVNALHDWHALSALNEKPLLDALDDLVRAGAPRRELEALRDAISLQ
                     LGYEIFMAADETKIELSRLLRAILSYHHASVDVDARVTRGRFEALIAPMLKDTETLLD
                     TVLAKAGIAAADVGEVVTTGGSSAIPAFRALLERHFPGSNVRAAAAFTSVAAGLAMPD
                     VPEAG"
BASE COUNT          430 a          929 c          938 g          386 t
ORIGIN      
        1 gcaggccgtc gcgttcgcgc gctcgatctc accgacggta aaggcggtgc acatcgccac
       61 caccgccgag agcgcggacg agttccgcga tcgctggcgg cgctggtcga gcgacatccc
      121 gctcgacgtg atcgagtcgc cctaccgttc cctgctgcag ccgctgctgc gctacatcga
      181 gcgcatcgac gaacgcgacg accggccgat cacggtggtc ctcgcggagt tcgtgccgcg
      241 caactggtgg gagtggatcc tccacagcca gacggcgcta cgcctcaagc tcgcgctgct
      301 cttccggccg aacacgatcg tcatcgacgt gccgtatcac ttcgcggata ccggccacgc
      361 gaagcggcgc gataaggacc ggcccgacag ctagagcgcg ctccgcgacg gcggaatatg
      421 ctcgcccctc catgcagctg cgacgcatcg tcgtgccact cgcggggacc agcgtcgacc
      481 ccgacgtcat ccgggtggcc gcgacgctcg cgaagccgac gaaggcggcg atcatcgcga
      541 tccacgtgat cgaggtgcgg tggaatctcc ctctggatgc ggtcctcgag gcggacaccg
      601 agcgcggcga gctcctcctg gacgaggcga tcaaggtcgc cgagcaagcg gggacgacga
      661 tcgagacgga gctgctgcag gcgcgcgagg ctgccgcggc gatcatcgac acggcgcgcg
      721 agcagaacgc ggatctcatc gtgctcggca tgccgttccg caaacgtctc gggcgcgtgt
      781 atatcggccg gaccgtacag caggtctacg tgggagcgcc gtgcgcggtg ctcgcgtatc
      841 gtcaggaaga ggttcgatga agatcgtcat catcggctgc ggccgagtcg gcgcgttcct
      901 ggcagggctc ctcgatgccg gcggtcacga ggtgaccgtc gtcgacctcg agcgatcggc
      961 cttcggccac cttcccgccg acttccacgg cacgaccctg ctcgggaatg gcaccgacat
     1021 ggaggtgctt cgtcaggcgg ggatcgaccg ggccgacgcg ctgctcaccc tgacccaggg
     1081 cgacaaccgc aacctcatgt cggcgcaggt cgcgaagcag atcttcggcg tgaagcaggt
     1141 catcgccaag gtcaacgacc cgatccgcgc caacacgtac cgcacgcacg gcatcgccac
     1201 gttctcgcgc acgagcatcc tcggcacgct gctcgaggcg atgctgatgg gcgaccccga
     1261 ggtcggcaag gtgctcgtgg agcgcgcgcg cggccacgag gcgcggctcg cggggcagag
     1321 ctagatgtac ttcgtcatcg cgggcggcgg cgaggtcggc ttccatctcg cgaaggccct
     1381 gctcgaggcg ggccacgaga tcatgctcat cgagagcgac cggcgccgcg cgaccttcat
     1441 ccaggagcag ctcgggtcgg tggtgctgaa cgcgcccgcg gacgaggggc gcttccagat
     1501 cgaggccggc tgccagcgcg ccgacgcggt cgtggccgtg accggcgagg accccgcgaa
     1561 cctcgtcatc tgccagctcg cgaagtggaa gtgcaacgtc ccgcgcgtga tcgcgcgcgt
     1621 gaacgacccg aagaacgaga tcgtgttcaa gacgctcggc atcgacgaga cgatctcgtc
     1681 gacgcgcgtc ctgatgaacg tcatcgagca ggagctgccc agcccgggtg gcttccttcc
     1741 gctcatgccg ctcaccgggt cgcatctcga gctcatcgag gccgaggtcg gtccgaacac
     1801 accgggggcg gggaacacca ttcgctcgct gggcctgccg cacggcaccg cggtcggggg
     1861 gatcgtgcgg aagggcagcg tgctccatgc cgacgaggac gtgcgcctcg aggtcggcga
     1921 tcgactggtc atcctggcgc cgaccaagga cgaggccgag gtccgaaagg ccctggcggg
     1981 ctgacacccg gtcaggcgat cgcgaccccc gctagcctgc ctccggcaca tccggcatgg
     2041 cgagccccgc ggccacgctc gtgaacgccg cggccgcgcg aacgttcgat ccggggaagt
     2101 gccgttcgag gagggcgcgg aatgccggta tcgcgctcga tccgccggtg gtcacgacct
     2161 cgccgacgtc cgcggcggcg atgccggcct tggccaggac ggtgtcgagc agtgtctcgg
     2221 tgtccttgag catcggggcg atgagcgcct cgaagcgacc gcgcgtgacg cgcgcgtcga
     2281 cgtcgacgga tgcgtggtgg tacgacagga tcgccctcag caggcgcgag agctcgatct
     2341 tcgtctcgtc ggcggccatg aagatctcgt agccgagctg cagcgagatc gcgtcgcgca
     2401 gtgcctcgag ctcgcgcctc ggtgcgcccg cacggacgag atcgtcgagc gcgtcgagca
     2461 gcggcttctc gttcagcgcc gacagggcgt gccagtcgtg gagcgcgttg acgatgaacg
     2521 cgggcagccg cagccgcttc gggccccact ccgcgcgcga gcccaggcgc ggcgcgacgg
     2581 cctcgtcgat gacgcgctcg gtacagcggt ccccgccgat gtcgcgcccc gcggtcgcga
     2641 tcacgcgcag cgcatcgccg tcccgccgca ccaccgcgag atc
//
LOCUS       DASTZR010000015         3654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_680041, whole genome shotgun sequence.
ACCESSION   DASTZR010000015 DASTZR010000000
VERSION     DASTZR010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680041"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VFV20_00340"
     CDS             <1..306
                     /locus_tag="VFV20_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286826.1"
                     /translation="MPEMWIYVPAVVVAALVGELVWRAMARGALGGRDARTGYWLIGA
                     AVPGVMFIGYFAMMSTLGGGIAWPTAMWTGAPVLAAVYGLLASVFAVPPRFATDTAG"
     gene            complement(334..636)
                     /locus_tag="VFV20_00345"
     CDS             complement(334..636)
                     /locus_tag="VFV20_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286827.1"
                     /translation="MVLRAGAALVAGASFVGATAYVVQHPKNPAAPLQPPTAGEIVPR
                     DAARPTATPAASGSPTPAPAQTGRVTPRASVRPPQITLQPGVRATELPGITYTHVS"
     gene            complement(651..1943)
                     /locus_tag="VFV20_00350"
     CDS             complement(651..1943)
                     /locus_tag="VFV20_00350"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEU5286828.1"
                     /translation="MVATLSKLNRRDFLRAGLVFGAGASGLAAGYAAVPDVFARAVYA
                     AKQEKVTNDRVLVMIQLAGGNDGLQTVMPLGNPAYRDLRPQLSKAESDALPISKELGL
                     NKNLRGVKSLFDQGKVAIVQGVGYPKPSFSHFDSIRVWETADPDRRQQDGWLGKTIAQ
                     NYDSAGHPLVGCACGTTGIPGALRDLEATLTVVNSQQSFKFNGGDEMDRAMGALYSGT
                     PGIYGALFDTAVTTARDTIAQLKTSATAYTPKSQYSDNAKLVYSSKNQLAAALQLASQ
                     LIVTGTGVKILHVTLGGFDTHYTQQNRHDDLMGYLDGAIASFYDDLAGHGMSDRVLVA
                     TWSEFGRRPKENQSAGTDHGTAAPVFLIGDTVKGGVYGEMPSLTRLDASGNLGYSVDF
                     RSVYQDILDTHLEVDAKEVFADSYERLAILKSSAVAKR"
     gene            complement(1954..3483)
                     /locus_tag="VFV20_00355"
     CDS             complement(1954..3483)
                     /locus_tag="VFV20_00355"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEU5286829.1"
                     /translation="MTDTKAAERRAEPTGSVASAAASPALNRRTVLAAATAAGVGVAA
                     ARLVAWANEPQFTPVPITGTLGNKTGLDWVSPLANESARVAHLLRRATFGATQDELQQ
                     AQSDGYARTVERLLDAKAVEPAPFPGGDEASSEKPLNIGQLQQWWIDHMLTTPTPFAE
                     RMTLFWHGHFTSDARKVGAQDPFLYWQNRTWRRFALSDLRTMLHEVTVDPAMLRYLDL
                     AQSTGRNPNENYSRELMELFTMGPEAYTEDDVRAAAKALAGWRTPLTQPVIDANLKRQ
                     MMQGGQTPKNPPKADAVRTGIFERNRSYTGPAFSFLGEFKVWNTDAVLDKILEQEATA
                     PFIVRKVLTHFATPTPSDAYVARLAASFRRSRYDVKALMRDVFMSPEFVAAPSYRALI
                     KTPTEFMVNAAKALGDRTVGRLILGNAQGMGQVLFDPPSVGGWPENESWISSNTMLAR
                     ANFVTAIVNTVKKLPPSGGGHDAYLDGVLSSQTLALLNQSSDDRRRWAVIFASPEFQL
                     K"
BASE COUNT          486 a         1216 c         1347 g          605 t
ORIGIN      
        1 atgcccgaga tgtggatcta cgtgccggcg gtcgtcgtcg ccgcactcgt cggtgagctc
       61 gtgtggagag cgatggcccg tggggcgctc gggggccgcg acgccagaac gggctactgg
      121 ctcatcggtg ccgccgtccc cggcgtgatg ttcatcgggt acttcgcgat gatgagcacg
      181 cttggcggcg gcatcgcttg gcccaccgcg atgtggaccg gcgcgccggt gctcgctgcc
      241 gtgtatgggc tgctcgcgag cgtgttcgcg gtcccgccgc gcttcgcgac ggacacggcc
      301 ggatagcacg cgcgcggctc gtcttctccg cggtcacgac acgtgcgtgt aggtgatccc
      361 gggcagctcg gtcgcgcgca cgccgggctg cagcgtgatc tgcggcggtc gcacgctcgc
      421 gcgcggcgtg acacggccgg tctgcgcggg cgcgggtgtc ggcgatccgc tcgccgcggg
      481 cgtcgcggtc ggccgcgcgg catcgcgcgg cacgatctcg cccgcggtgg gcggctgcag
      541 cggcgccgcg gggttcttgg gatgctgcac gacgtaggcg gtcgcgccga cgaagctcgc
      601 gcccgcgacc agcgcggcgc ccgcgcgcag caccacggac cggtccacgg tcagcgtttc
      661 gcgacggcgg aggacttgag gatcgcgagc cgctcgtagg agtcggcgaa cacttccttc
      721 gcgtcgacct cgaggtgcgt gtcgaggatg tcctggtaca ccgagcggaa gtcgacgctg
      781 tagccgaggt tgccgctcgc gtcgagcctc gtgaggctcg gcatctcgcc gtacacgccg
      841 cccttcaccg tgtcgccgat gaggaagacc ggcgcggcgg tcccgtggtc ggtgcccgcg
      901 ctctggttct ccttcggccg ccggccgaac tcggaccagg tggcgacgag cacccggtcg
      961 gacatgccgt ggccggcgag gtcgtcatag aaggacgcga tcgcgccgtc gaggtagccc
     1021 atgaggtcgt cgtggcggtt ctgctgcgtg tagtgcgtgt cgaagccgcc gagcgtcacg
     1081 tggaggatct tcacgccggt cccggtgacg atgagctgcg aggcgagctg cagcgccgcc
     1141 gccagctggt tcttggagga gtacacgagc ttggcgttgt cggagtactg ggacttcggc
     1201 gtgtacgcgg tggccgacgt cttcagctgc gcgatcgtgt cgcgcgccgt cgtgaccgcg
     1261 gtgtcgaaca gcgcgccgta gatgccgggc gtgcccgagt agagcgcgcc catggcccgg
     1321 tccatctcgt cgccgccgtt gaacttgaac gactgctgcg agttcaccac ggtgagcgtc
     1381 gcctcgagat cgcgcagcgc gccggggatg ccggtggtcc cgcacgcgca gccgacgagc
     1441 ggatggccgg cggagtcgta gttctgcgcg atggtcttgc cgagccagcc gtcctgctgc
     1501 cggcgatcgg gatcggccgt ctcccacacg cggatcgagt cgaagtgcga gaaggacggc
     1561 ttcggatagc cgacgccctg gacgatcgcg accttgccct gatcgaagag cgacttcacg
     1621 ccccggaggt tcttgttgag cccgagctcc ttcgagatcg gaagcgcgtc gctctccgcc
     1681 ttcgaaagct gcggacgcag gtcgcggtac gccggattcc cgagcggcat cacggtctgc
     1741 agcccgtcgt tcccgccggc gagctggatc atcacgagca ccctgtcgtt cgtgaccttc
     1801 tcctgcttcg ccgcgtacac cgcccgggcg aagacgtcgg gcaccgcggc gtatccggcg
     1861 gcgagccccg atgcgccggc cccgaacacg agcccggcgc gcaggaagtc ccggcgattc
     1921 agcttcgaga gcgtggcgac catgtcggcc tcctcatttc agctggaact cgggcgaggc
     1981 gaagatgacg gcccaccgcc gccggtcatc gctcgactgg ttcagcagcg cgagggtctg
     2041 tgacgagagc acgccgtcga gatacgcgtc gtgcccgccg cccgacggcg ggagcttctt
     2101 caccgtgttg acgatcgcgg tgacgaagtt cgcgcgcgcg agcatggtgt tcgaggagat
     2161 ccacgattcg ttctccggcc agccgccgac gctcggcgga tcgaagagca cctggcccat
     2221 gccctgcgcg ttcccgagga tgaggcgccc gaccgtgcgg tcgcccagcg ccttcgccgc
     2281 gttcaccatg aattcggtcg gcgttttgat gagggcgcgg tacgacggcg ccgcgacgaa
     2341 ctcggggctc atgaacacgt cgcgcatcag ggccttcacg tcgtagcggc tccggcggaa
     2401 gctcgccgcg aggcgtgcga cgtacgcgtc ggagggtgtc ggcgtcgcga agtgggtgag
     2461 gaccttccgc acgatgaacg gcgccgtcgc ctcctgctcg aggatcttgt cgagcacggc
     2521 gtcggtgttc cagaccttga actctccgag gaacgagaac gccgggccgg tgtaggagcg
     2581 gttgcgctcg aagatcccgg tgcgcaccgc gtcggccttc ggcgggttct tgggcgtttg
     2641 gccgccctgc atcatctgcc gcttcaggtt cgcgtcgatc accggctggg tgagcggcgt
     2701 ccgccacccg gcgagcgcct tcgcggcggc gcgcacgtcg tcctcggtgt aggcctcggg
     2761 ccccatggtg aagagctcca tgagctcgcg ggagtagttc tcgttgggat tcctgcccgt
     2821 ggactgcgcg aggtcgaggt agcgcagcat cgccgggtcc accgtgacct cgtggagcat
     2881 cgtccgcagg tcggagagcg cgaagcggcg ccaggtccgg ttctgccagt agaggaacgg
     2941 gtcctgcgcg ccgaccttgc gcgcgtccga cgtgaagtgc ccgtgccaga agagcgtcat
     3001 gcgctccgcg aacggcgtcg gcgtggtgag catgtggtcg atccaccact gctggagctg
     3061 gccgatgttg agcggcttct cggagctcgc ctcgtcgccg ccggggaagg gcgcgggctc
     3121 cacggccttc gcgtcgagca gacgttccac ggtcctcgcg taaccgtcgg actgcgcctg
     3181 ctgcagctcg tcctgcgtcg cgccgaacgt cgcgcggcgc aggagatgcg cgacgcgtgc
     3241 ggactcgttc gcgagcggcg agacccagtc gagcccggtc ttgttgccga gcgtgccggt
     3301 gatcggcacc ggcgtgaact gcggctcgtt ggcccacgcg acgaggcgcg ccgccgcgac
     3361 acccacgccc gcggccgtcg cggccgcgag gaccgtgcgg cggttcagcg ccgggctcgc
     3421 ggcggcggac gcgaccgagc ccgtgggctc cgctcggcgc tcggctgcct ttgtgtcggt
     3481 catgtgtctg gggagagtac gcgggcggtc cgccaaagtg gcgccccgca agggggtggc
     3541 tacacgcgtt taccgacttc ttgaaatcgg gcgggtccac ggcccgcgga acggccggga
     3601 tctttgaggg tgcggcccgc cggagcgggg tgagggaacc ctattgacaa gacc
//
LOCUS       DASTZR010000016         2033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_549286, whole genome shotgun sequence.
ACCESSION   DASTZR010000016 DASTZR010000000
VERSION     DASTZR010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549286"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="VFV20_00360"
     CDS             complement(<1..962)
                     /locus_tag="VFV20_00360"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5286830.1"
                     /translation="MTLFRLALRHQRTGLIVMSTMSLLAGALNAAAFEQLAGTTPAER
                     AVFGQQMELLGKQLSFLLPDPIQLDTMAGYLTWRAFGFLSLVFAIWAILASTGAGRGE
                     EERGLTEHWLATGVSRLRWIATRTAGFVVSSVIVIVVTLGGTALVAAAVNDPLVTGSV
                     IVEGVEFLGLTLVAFGIGLLIAQLVPTRRAAASIGAVAIIALYLLNSASRSGLEVGWL
                     SNVSPFALFDRSRPLLPRGGDVDLAATIALYVTALILVAVSVWAFLRRDVGGVLVRLR
                     NERTRATGRPSRDPFLRMPVLAMVDQQRWWLAGWTVGLALLAYFL"
     gene            complement(959..1840)
                     /locus_tag="VFV20_00365"
     CDS             complement(959..1840)
                     /locus_tag="VFV20_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020521618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5286831.1"
                     /translation="MTDAIHSDGLTKDYGSGRGIFDLDLRIAEGEIFGYLGPNGAGKT
                     TTIKLLMGMIHATRGAASVFGLDTDRDSVALKHRIGYVPGELPQFGGWRGTEIVAYLA
                     GLRGGVADSEVERIAKLLDLDLSRKYREYSHGNKQKLALLLAFVPRPAVLILDEPTSG
                     LDPLHQQEFYGLIRDARESGATIFVSSHVLSEVEQICDRVGIVREGHLITVGPIGELL
                     GAKAHRVDIELADAAVAEAIGRVPGLEQVRIDGPRVSGLYRGSFDPLLAALARERVVS
                     FVSREPSLDEIFLSYYR"
     gene            complement(1837..>2033)
                     /locus_tag="VFV20_00370"
     CDS             complement(1837..>2033)
                     /locus_tag="VFV20_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286832.1"
                     /translation="TAQTLALFERLREGATVATAPSVRRKLNGMTSALESAFADIPQI
                     ARHAQARHAQARHAQARRRA"
BASE COUNT          327 a          717 c          706 g          283 t
ORIGIN      
        1 aggaagtacg cgaggagcgc gagaccgacg gtccagcccg cgagccacca ccgctgctgg
       61 tcgaccatgg cgagcacggg catccgcagg aacggatcgc gcgatggtcg tccagtcgct
      121 cgcgtgcgct cgttccggag ccggacgagg acgccgccga cgtcccgacg caggaacgcc
      181 cagacgctca ccgcgaccag gatcagcgcg gtgacgtaca gcgcgatcgt ggcggccagg
      241 tcgacgtcgc ctccgcgtgg gaggagtggc cggctgcggt cgaagagcgc gaagggcgag
      301 acgttgctga gccacccgac ctcgagcccc gaacggctcg cggaattcag caggtagagc
      361 gcgatgatcg cgacggcgcc gatcgacgcc gccgcgcgcc gcgtgggcac gagctgcgcg
      421 atgagcaggc cgatgccgaa cgccacgagc gtgaggccga ggaactcgac gccctcgacg
      481 atcacgctgc cggtcacgag gggatcgttc accgcagcgg cgaccagcgc cgtcccgccg
      541 agggtcacga caatgacgat gacgctggag accacgaagc ccgcggtgcg cgttgcgatc
      601 caccgcagcc gcgagacacc ggtcgcgagc cagtgctcgg tgagcccgcg ctcctcctcg
      661 ccgcggccgg cgccggtcga cgcgaggatc gcccagatcg cgaagaccag cgagaggaag
      721 ccgaacgcgc gccacgtcag gtagcccgcc atcgtgtcga gctgtatcgg gtcgggaagc
      781 aggaacgaga gctgcttgcc gaggagctcc atctgctgac cgaatacggc gcgctccgcg
      841 ggcgtcgtcc cggcgagctg ctcgaacgcc gccgcgttca gcgcgccggc aagcaggctc
      901 atcgtcgaca tcacgatgag tcccgtccgc tgatggcgca gcgcgagacg gaagagcgtc
      961 atcggtagta gctcaggaag atctcgtcga gcgacggctc gcgcgacacg aagctcacca
     1021 cccgctccct ggcgagcgcc gcgagcaacg gatcgaagct cccgcggtac aggccgctca
     1081 cgcgcgggcc gtcgatccgc acctgctcca ggcccggcac gcgcccgatc gcctcggcca
     1141 cggcggcgtc ggcgagctcg atgtcgacgc gatgcgcctt cgcaccgagc agctctccga
     1201 tcgggccgac cgtgatgagg tgaccctcgc gaacgattcc cacgcggtcg cagatctgct
     1261 cgacctcgga cagcacgtgc gaagacacga agatcgtcgc cccgctctcg cgcgcgtcgc
     1321 ggatgagccc gtagaactcc tgctgatgga ggggatcgag tccgctcgtc ggctcgtcca
     1381 ggatgaggac ggcgggccgc ggcacgaacg cgaggagcag cgcgagcttc tgcttgttcc
     1441 cgtgggagta ctcgcggtac ttgcgcgaga gatcgaggtc cagcagcttc gcgatgcgct
     1501 cgacctcgct gtccgcgacg ccaccgcgca gaccggcgag gtacgcgacg atctccgtcc
     1561 cgcgccatcc gccgaactgc ggcagctcgc cgggcacgta gccgatccgg tgcttcaggg
     1621 cgacggagtc ccggtcggtg tcgagcccga agacggacgc cgctccgcgc gtcgcgtgga
     1681 tcatgcccat cagcagcttg atcgtcgtcg tcttgcctgc gccgttcggt ccgaggtagc
     1741 cgaagatctc gccttccgcg atccgcaggt cgagatcgaa gatcccgcgc ccgctgccgt
     1801 agtccttggt gaggccgtcg ctgtggatcg cgtccgtcat gcccttctcc ttgcctgtgc
     1861 gtgccgcgcc tgcgcgtgcc tcgcctgcgc gtgccgcgcg atctgtggga tgtccgcgaa
     1921 cgcgctctcg agcgcgctcg tcatgccgtt cagcttccgc cgtaccgagg gcgcggtcgc
     1981 gaccgtggcg ccttcacgaa gccgctcgaa cagcgcgagg gtctgcgcgg tgc
//
LOCUS       DASTZR010000017         2198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_497430, whole genome shotgun sequence.
ACCESSION   DASTZR010000017 DASTZR010000000
VERSION     DASTZR010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497430"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            288..1619
                     /locus_tag="VFV20_00375"
     CDS             288..1619
                     /locus_tag="VFV20_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5286833.1"
                     /translation="MATLLDETTDLLQHLIRNECVNDGTVESGHEMRSVKTLESYLQM
                     PGVEMKRYEPAPGRGSLVLRIEGTDKTAPSLHLMGHTDVVPVTRAGWREDPFGGELID
                     GEVWGRGAIDMLDVTASMAVAVKQLLSTGFRPKGTLIYSACADEEALGTWGAKWLTEN
                     AWDDVKSTYLVTEFGGARLPIGQGAKLPIMVAEKGSQWTRLRVKGTPGHGSMPYKSDN
                     ALVKASELITRIARYKAPLHLQGIWKQFVESIDLPAVQRIALTNGASFDAALERMPPG
                     TDRMMYAATRTTFSPNIAHAGVKLNVIPDSAEVDVDIRLLPGDEGEGVRRMLRDAAGD
                     LWKDVEIVQEGDNPATESPTNTVLWDALTRVTQRLIPNSTTVPFLVVGATDARYFRRK
                     GVVAYGYGLMSEKIPFGEFSKLFHGNNERVDQETLSLMTPLWDGVVREMVG"
     gene            complement(1616..2041)
                     /locus_tag="VFV20_00380"
     CDS             complement(1616..2041)
                     /locus_tag="VFV20_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEU5286834.1"
                     /translation="MRGSCAFCAVVAGADHHAVYRDDATVAFLDHAPVLKGHTLVVPT
                     AHYETLDDLPDDALAPLFATVRRVSIALQRALDAEGSLMLSNTRISQSVPHVHVHVLP
                     RNKGDHLFSPGRTLWMRQRYAAGEAEDIAAKLRAALAAA"
BASE COUNT          364 a          743 c          793 g          298 t
ORIGIN      
        1 cgaggcggcg gacggcatcc gcagggggct gaagtacgtc accgcggaca agctgctgcc
       61 gcatccggac tgcggtctca agacgcggac ggtcgaggag tcgctcgaca agtgccgtgt
      121 cgtggtcgaa gccgcgaaga aggtgcgcgc cgagatcgcg aagacgagcg ccgtgtagct
      181 gcccgccacc ggacgggcgc gggcgggcgg cggcgtacgc gcgcctctgc tcgtacattg
      241 gagcgggacc cggacgtctt ccatcgaccg cttcggaggt gcgcgccatg gccacgcttc
      301 tcgatgagac caccgatctg ctgcagcacc tgatccgcaa cgaatgcgtc aacgacggca
      361 cggtcgagtc gggccacgag atgcgcagcg tcaagacgct cgagagctac ctgcagatgc
      421 cgggcgtcga gatgaagcgc tacgagcccg cgccgggccg cggcagcctg gtcctccgga
      481 tcgagggcac cgataagacc gcgccgagcc tccatctgat gggccacacc gacgtcgtgc
      541 cggtgacgcg tgccgggtgg cgcgaggatc cgttcggcgg cgagctcatc gatggcgagg
      601 tgtggggccg cggcgcgatc gacatgctcg acgtcaccgc ttccatggcc gtcgcggtga
      661 agcagctcct ctcgaccggc ttccggccaa agggcacgct catctactcc gcgtgcgccg
      721 acgaggaggc gctcgggacg tggggcgcga agtggctcac cgagaacgcc tgggacgacg
      781 tgaagagcac ctatctcgtc accgagttcg gcggcgcgcg actgcccatc gggcagggcg
      841 cgaagcttcc gatcatggtg gccgagaagg ggtcgcaatg gacccgtctc cgggtgaagg
      901 gcacgccggg ccacggctcg atgccgtaca agtcggacaa cgcgctggtg aaggcgtcgg
      961 agctcatcac ccgtatcgcg cggtacaagg cgccgctcca cctccagggc atctggaagc
     1021 agttcgtcga gagcatcgac ctgccggcgg tgcagcgcat cgcgctgacg aacggcgcgt
     1081 cgttcgacgc cgcgctcgag cgcatgccgc ccggcaccga tcgcatgatg tacgccgcca
     1141 cgcggaccac cttctccccg aacatcgcgc acgccggcgt gaagctgaac gtcatccccg
     1201 acagcgccga ggtcgacgtc gacatccgcc tccttcccgg agacgagggc gagggcgtcc
     1261 gccggatgct gcgtgacgcc gccggtgacc tgtggaagga cgtcgagatc gtccaggaag
     1321 gggacaaccc ggccaccgag tcgccgacca acaccgtcct ctgggacgcg ctcaccaggg
     1381 tgacgcagcg cctcatcccg aactcgacga cggtgccgtt cctcgtggtc ggcgccacgg
     1441 acgcgcgcta cttcaggcga aagggcgtcg tcgcgtacgg atacggcctg atgagcgaga
     1501 agatcccctt cggcgagttc tcgaagctct tccacggcaa caacgagcgc gtggaccagg
     1561 agacgctctc gctgatgact ccgctctggg acggcgtcgt ccgcgagatg gtcggctagg
     1621 ccgcggcgag cgccgcgcgc agcttcgcgg cgatgtcctc ggcctcgccg gcggcgtagc
     1681 gctggcgcat ccagagcgtc cggccgggcg agaagagatg gtcgcccttg ttccgcggca
     1741 acacgtgcac gtgcacgtgc ggcacgctct ggctgatgcg cgtgttcgag agcatgagcg
     1801 acccctccgc gtccagggcc cgctgcagcg cgatcgacac gcgacgcacg gtcgcgaaga
     1861 gcggcgcgag cgcgtcatcc gggaggtcgt cgagcgtctc gtagtgcgcc gtggggacga
     1921 cgagcgtgtg gcccttgagc accggcgcgt ggtcgaggaa cgcgaccgtc gcgtcgtcgc
     1981 ggtagacggc gtgatgatcg gcgccggcga cgacggcgca gaacgcgcac gatccgcgca
     2041 tcagcggagc cgtgtcgcca tcggcaccgg cgtgccgcgc gccggcgtga ccgtcgccga
     2101 cggggagcgc atcagccgca cgacgttggc ggcgaaggtc gcgagcgcca ggtacgcgag
     2161 cacgcccgag cccgcgacga cggcgttcgc gagcgcgt
//
LOCUS       DASTZR010000018         3794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_314334, whole genome shotgun sequence.
ACCESSION   DASTZR010000018 DASTZR010000000
VERSION     DASTZR010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314334"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1132)
                     /locus_tag="VFV20_00385"
     CDS             complement(<1..1132)
                     /locus_tag="VFV20_00385"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU5286835.1"
                     /translation="MLQPPLAGIRVLEVGNYMAGPYCGMQLADLGADVVKIEDPRGGD
                     LSRRLEPMVDGESGNFVRINRSKRSVALDLKRDSAREVFRRLAARSDVIVENLRPGTM
                     GDLGLDPRDLLDANPRLIYAAVTGWGLSGPYADRPALDIIVQAMSGLMSVTGEEGGAP
                     VKVGVSISDLTAGLYATIAVLAALAVRARDGRGQLVDVSMFESSASLAVWEAGTYFTT
                     GETPRAAGSAHKLLAPYQAVRAADGHFVVGATTPPNWTAFCRAMGLVSLEHDPRFADG
                     TLRRARVAELIPLIEAVTTTRPAAHWLALLRDAGVPCAEIQDYAAVFTDPHLVDRGFF
                     VDLPHPRLGTVRALGTPIRLASTRVGPERAGPLLGEHTREVLA"
     gene            complement(1160..2158)
                     /locus_tag="VFV20_00390"
     CDS             complement(1160..2158)
                     /locus_tag="VFV20_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286836.1"
                     /translation="MRSSVAAGLAILAVLAAIGGGLAFSRIARAPAPTPRRVSVTATP
                     AVTPSPAPFDEAALFRQPLSAGCATSQSVWIITNGGGLLRYDGSAWEQVDDTLRSLTA
                     VGCTPAAAVAVGLVGAMVTIDERTRQIRSQDISLNDLFGVSPVGDGALMVGSDGAVYI
                     LAGGETQPYARGLDEHLRDVVAFSLTSAWAVGADGITYRLDQRGWNPVGSGQTATLRA
                     VAAATAAAPVAVGDAGTIVTYAGGGWHTVKSGVTATLRDLIVEPAVWIAGDGGTLLRG
                     SLDELRPVDLHTSCDLVSIFSGPTRGDVWVVGVRGGGGGVWALAADGSVIKHWGGC"
     gene            complement(2178..3101)
                     /locus_tag="VFV20_00395"
     CDS             complement(2178..3101)
                     /locus_tag="VFV20_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEU5286837.1"
                     /translation="MAAGQLRLNIYDAAAANRWRTVLLIAVFTLVVALLAYLGGEYLA
                     PGYGPAMLPFAAGLSGVSAAASYFAGDKLVLAQSQARRLAADEEPELRSVVESLSIGL
                     GIQPPALYVIEDSAPNAFATGRDPAHASVAVTRGLLDKLERAELEGVIAHELSHVGNR
                     DIRVMLLVTVLVGTVALISDWLLRSMWWGGGRRRDRDRGGGSAIIAIIAIALAILTPV
                     IATLLQFAVSRQREYLADASGALLTRYPPGLAGALRKIASDPEPLEVANKATAPLYIA
                     NPLHDAPRFMDSLFNTHPPIQERIRRLEAMG"
     gene            complement(3108..3671)
                     /locus_tag="VFV20_00400"
     CDS             complement(3108..3671)
                     /locus_tag="VFV20_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU5286838.1"
                     /translation="MEIWIILAIVAAIAFFLIATYNRLVTLRQRVKEAWADIDVQLKR
                     RWDLIPNLVETVKGYAAHERGTFEAVTQARSAAQAAAQTGSPEARAQAENALGAALLN
                     LRAVAEAYPQLQAVQEFKDLSENLRDTEDKIQFARRFYNGNVRDYNTALQTFPTNTLA
                     GMFGFTSEQYFELTDAAQRETPKVSFS"
BASE COUNT          550 a         1339 c         1392 g          513 t
ORIGIN      
        1 cggcgaggac ctcgcgggtg tgctcgccga ggagcggtcc cgcgcgctcc ggtccgacgc
       61 gcgtcgaggc gagccggatc ggcgtgccga gcgcgcgcac ggtcccgagg cgcggatgcg
      121 ggaggtcgac gaagaaccca cggtcgacca ggtgggggtc ggtgaacacc gcggcgtagt
      181 cctggatctc ggcgcacggc acgcccgcgt cgcggagcag cgcgagccag tgcgccgccg
      241 gacgagtggt ggtgacggcc tcgatgagcg ggatgagctc ggccacacgc gctcgtcgca
      301 gcgtcccgtc ggcgaaccgc gggtcgtgct ccagcgagac gaggcccatc gcgcggcaga
      361 acgccgtcca gttcggcggc gtcgtcgcgc cgacgacgaa gtggccgtcc gcggcgcgga
      421 cggcctggta cggcgcgagg agcttgtgcg cggacccggc ggcgcgcggg gtctcgccgg
      481 tcgtgaagta cgtgccggcc tcccagaccg cgagcgaggc cgacgactcg aacatcgaga
      541 cgtcgacgag ctggccgcgc ccgtcgcgcg cgcggaccgc gagcgccgcg agcaccgcga
      601 tggtcgcgta gaggccggcg gtgagatccg agatcgagac accgaccttc accggcgccc
      661 cgccctcctc gcccgtcacg gacatgagcc cgctcatcgc ctgcacgatg atgtcgaggg
      721 cgggccggtc ggcgtacgga ccggagaggc cccagccggt gaccgccgcg tagatgaggc
      781 gcgggttcgc gtccagcaga tcgcgcggat cgaggcccag gtcgcccatg gtgccgggcc
      841 gcaggttctc cacgatcacg tcgctgcgtg ccgcgaggcg ccggaagacc tcgcgcgcgc
      901 tgtcgcgctt caggtcgagc gccacgctgc gcttcgagcg gttgatccgt acgaagttgc
      961 cgctctcgcc gtcgaccatc ggctcgagcc gccgcgaaag gtcgccgccg cgcggatctt
     1021 cgatcttcac cacgtcggcg ccgaggtcgg cgagctgcat gccgcagtac ggacccgcca
     1081 tgtagttgcc gacctcgagc acgcggatgc ccgcgagcgg cggttggagc atggccgcga
     1141 gtctaggtgc ggccgccgct cagcacccgc cccagtgctt gatgaccgac ccatcggccg
     1201 cgagcgccca cacgccgccc ccgcccccgc gcacaccgac gacccagacg tccccgcggg
     1261 tcggcccgga gaagatcgac acgaggtcgc agctggtgtg gaggtcgacg ggccgtagct
     1321 cgtcgagcga tccgcgcagc agcgtgccgc cgtcaccggc gatccagacg gcgggttcga
     1381 cgatcaggtc gcgcagcgtc gcggtcacac cgctcttcac cgtgtgccag ccgccgcccg
     1441 cgtaggtgac gatcgtgccg gcgtcaccga cggcgaccgg cgcggccgcg gtggccgcgg
     1501 ccaccgcccg aagcgtggcc gtctgccccg atccgacggg gttccaccca cgctggtcga
     1561 gccggtaggt gatgccgtcc gcgccgaccg cccatgccga cgtcagcgag aacgcgacga
     1621 cgtcgcggag gtgctcgtcg aggccccgcg cgtacggctg cgtctcgccg cccgcgagga
     1681 tgtacacggc gccgtccgat cccaccatga gcgcgccgtc gccgacgggc gacacgccga
     1741 agaggtcgtt cagcgagatg tcctgcgagc ggatctgccg ggtccgctcg tcgatcgtca
     1801 ccatcgcgcc gaccaggccg acggcgacgg cggccgcggg cgtgcatccg acagcggtga
     1861 gcgagcgaag cgtgtcgtcg acctgctccc acgcgctgcc gtcgtaccgc agcagcccgc
     1921 cgccgttggt gatgatccag accgactgtg acgtcgcgca gcccgccgag aggggctgcc
     1981 ggaacagcgc ggcctcgtcg aacggtgccg ggcttggggt gacggccggc gtcgcggtca
     2041 cgctcacgcg ccgcggggtc ggcgcgggcg cgcgggcgat gcggctgaac gcgaggccgc
     2101 cgccgatcgc ggcgagcacg gcgaggatcg cgaggcccgc ggcaacactc gatctcatcg
     2161 cggcagcggg gcgggcatca gcccatcgct tcgagccggc ggatgcgctc ctggatcggt
     2221 gggtgcgtgt tgaagaggct gtccatgaag cgcggcgcgt cgtggagcgg gttcgcgatg
     2281 tacagcggcg cggtcgcctt gttcgcgacc tcgagcggct cgggatccga cgcgatcttg
     2341 cgcagcgcgc cggcgagccc cggcgggtac cgcgtgagca gcgcgccgga cgcatccgcg
     2401 aggtactcgc gctgccgcga gacggcgaac tggaggagcg tcgcgatcac cggcgtcagg
     2461 atcgcgagcg cgatcgcgat gatcgcgatg atcgccgagc caccaccgcg gtcgcgatcg
     2521 cggcgcctcc ccccgcccca ccacatcgag cggagcagcc agtccgagat cagcgcgacg
     2581 gtccccacaa ggacggtaac gaggagcatg acgcggatgt cgcggttccc cacgtgggag
     2641 agctcatgcg cgatcacgcc ctccagctcc gcgcgctcga gcttgtccag gaggccacgg
     2701 gtcacggcga cggaggcgtg cgccggatcg cgaccggtcg cgaacgcgtt cggcgccgag
     2761 tcctcgatga cgtagagggc cggcggctgg atcccgaggc cgatcgagag cgactccacc
     2821 acgcttcgga gctccggctc ctcgtccgcc gcgagccgcc gggcctggct ctgcgcgagg
     2881 accagcttgt cgccggcgaa gtacgacgcc gcggccgaga cgccggacag gccggccgcg
     2941 aacggcagca tcgcgggccc gtagccgggc gcgaggtact cgcctccgag gtacgcaagg
     3001 agcgcgacga ccagcgtgaa caccgcgatg agcagcaccg tccgccaccg gttcgcggcg
     3061 gccgcgtcgt agatattcag gcggagctgc ccagcggcca cgtcgaccta gctgaacgac
     3121 acctttggcg tctcacgctg cgccgcgtcc gtgagctcga agtactgctc ggacgtgaac
     3181 ccgaacatgc ctgcgagcgt gttcgtcggg aacgtctgga gcgcggtgtt gtagtcacgc
     3241 acgttgccgt tgtagaagcg ccgcgcgaac tggatcttgt cctcggtgtc gcggaggttt
     3301 tcggacagat ccttgaattc ctggacggcc tgcagctgcg gatacgcctc ggcgacggca
     3361 cgcaggttca ggagggccgc gccgagggcg ttctcggcct gcgcgcgcgc ctcgggcgat
     3421 cccgtctgcg ccgcggcctg cgccgcggag cgcgcctgcg tgaccgcctc gaaggtcccg
     3481 cgctcgtgcg ccgcgtaccc cttcacggtc tcgacgagat tcgggatgag gtcccagcgc
     3541 cgcttcagct ggacgtcgat gtccgcccac gcttccttca cgcgctgacg gagcgtgacc
     3601 aggcggttgt aggtggcgat gaggaagaac gcgatggccg cgacgatcgc gaggatgatc
     3661 cagatctcca cgctgctccc cttccttgag ggtttcgctg ctcgcaatga tgacaggttg
     3721 ggtgagctgg cgagtcgcta tagcaatgcg cgcgcagcgt tcgcgatgca gtcggcgtcg
     3781 atgcccgcct cgtg
//
LOCUS       DASTZR010000019         5001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_732580, whole genome shotgun sequence.
ACCESSION   DASTZR010000019 DASTZR010000000
VERSION     DASTZR010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5001
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732580"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VFV20_00405"
     CDS             <1..243
                     /locus_tag="VFV20_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5286839.1"
                     /translation="SIVKGTKNLAAAQALVNWSLTKEAGELNVKLSNRGSVRTDVTPA
                     AGSPKLSDVKMVNYDRQWATDNKDRILKAWQQAVGL"
     gene            270..1889
                     /locus_tag="VFV20_00410"
     CDS             270..1889
                     /locus_tag="VFV20_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU5286840.1"
                     /translation="MRSLQREPGVLLALLAVITLVGVFILYPQVRVILTPGTGYVDFI
                     TGGTWVRSTWQSIQVMLLSTTSAVLLGFVFAYAMVYTNMRWKPFFRVIGILPLLSPPF
                     VVAASYVILFGPRGIITYGVFGQTISVFGLGGIWGVQTIAFFPFAYQLIADVLSRSDA
                     RLEQAARNLGAGPWQVFRSVTLPLSRPGLGAAILTTAIYVLEDFGNPALIGGTYTVLP
                     TQAYGLISGFGDLPGATAVSTLLLALALVLYFAKIRLDGGRSFITVSGRASSMPRPPV
                     PAALSWACFAACAALSIVIITVYGSLVVSALTLTFPTNLGFTTKHFEYITSGPNGTAL
                     QNTLVFGLVAAVFSALFALVAGWLVERGAWKGRGVLDFLLIMPAAIPGLFFGIGYATA
                     FNEAWLDWLDRGVLIIISMIFWNIPVGYQAAVAGLRQIDRSMDEAATSLGASSLRGFR
                     DVLLPMLGGSLRVGFVTTFVRAVTTLSVVIFLFTPSSTVATIRIFQLVNDLNWGAATA
                     FTVADIGMAIVALSVISLLARGRIALGAARA"
     gene            1882..2976
                     /locus_tag="VFV20_00415"
     CDS             1882..2976
                     /locus_tag="VFV20_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5286841.1"
                     /translation="MRESPAKAGEIHLRLAGVTKRFGETVAVDDVSFEIPRGSFATLL
                     GPSGCGKTTTLRMIAGFYDPDAGDIILGGRRINELPAHRRGTAMVFQDYALFPHMTVR
                     DNVGYGLRVANIAKDERERRVNETLEFVGLGKLGDRWPNQLSGGQQQRVAVGRALVLR
                     PEILLLDEPLSNLDAKLRVQLRWELRALQQQLGMTFVYVTHDQDEALSLSDWIAVMNA
                     GKVEQHGTPWEIYYHPRTAFLADFVGAVNLVPGSVRELQGSSAVVSFGKRALTVRVPA
                     GLTVTPGSGVKLCIRPEALALAPSAAGDGEAFPGRVARKAFLGDLMRYWVVVDGQEWI
                     VDQPDPGATSPFEGDVLVRVRPERVHLIAG"
     gene            complement(2985..3722)
                     /locus_tag="VFV20_00420"
     CDS             complement(2985..3722)
                     /locus_tag="VFV20_00420"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5286842.1"
                     /translation="MTIGVRFLGSGNAFADGGRSHACVHVTAPGVSLLLDCGGSALPA
                     ITRAGLADGIDAIAVSHLHGDHFGGIPYFVIQQHFAGRTRPLTIGGPRELAARLHASE
                     QGLYPDFFGGTRLGFEVRVVPLGADEIDLGGARVSALPVDHVPLSDPHGLRVRVAGRL
                     VAYSGDATWTDRLPEVARGADLFICEATNFDTDDPSHLSYRTLMKHRADLDCRRIVLT
                     HLGTSTLAHLAELELDYATDGKTLELD"
     gene            complement(3719..4714)
                     /locus_tag="VFV20_00425"
     CDS             complement(3719..4714)
                     /locus_tag="VFV20_00425"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5286843.1"
                     /translation="MRIGLLGVGRLGAAHAATLRALPDVADLAIYDADAARANEVAGK
                     VGGRAVDTIDAALDGADAVVIVTPTDTHAPLIRRCLDARIATFCEKPIAIGIDETRGV
                     VAHAERTNGRLQIGFQRRFDAGYGSAKDALSSGRLGPVYSFVMVSCDRLPPPDAYIAT
                     SGGQFKDQLIHDFDITRWLFDDEVEEVTATGSTLGLPQYEGMRDVATSAVLVKMRGGS
                     LGLITGLRHNEAGYDIHVEVHAAKDTLAIGIDPRTPWRSVEADAPALAGPAYPSFFVR
                     FADAYRAELAHFLRFARGDAPNACTAADALEALRIAEAAGRSWRERRTVKVSEIA"
     gene            4761..>5001
                     /locus_tag="VFV20_00430"
     CDS             4761..>5001
                     /locus_tag="VFV20_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosose dehydratase"
                     /protein_id="HEU5286844.1"
                     /translation="MQLAAAPISWGVCEVPGWGYQLPPDRVLADMRRLGLRDIEAGPP
                     GFLPADDAAARALVARLDLRVVAAFVTAVLHRRDVL"
BASE COUNT          682 a         1769 c         1760 g          790 t
ORIGIN      
        1 agcatcgtga agggcacgaa gaacctcgcg gcggcgcagg cgctcgtgaa ctggtcgctg
       61 acgaaggagg ccggcgagct gaacgtgaag ctcagcaacc gcggctccgt gcgcaccgat
      121 gtcacaccgg cggccggctc acccaagctc tccgacgtga agatggtgaa ctacgaccgc
      181 cagtgggcga cggacaacaa ggaccgcatc ctgaaggcgt ggcagcaggc ggtagggctc
      241 tgatcgcggc cgcgaacctg atctagacga tgcgcagctt gcagcgcgag ccgggtgtcc
      301 tgctcgcgct gcttgctgtc atcacgctgg tcggcgtctt catcctgtac ccgcaggtgc
      361 gcgtgatcct cacgccgggc accggctacg tggatttcat caccggcggg acgtgggtcc
      421 gctccacctg gcagtcgata caggtcatgc tcctgtccac cacgagcgcg gtgctcctcg
      481 gattcgtgtt cgcgtacgcg atggtctaca cgaacatgcg ctggaagccg ttcttccgcg
      541 tcatcggcat cctgccgctg ctgtcgccgc cgttcgtcgt ggccgcgtcc tacgtcatcc
      601 tcttcggacc gcgcggcatc atcacctacg gcgtcttcgg ccagacgatc agcgtcttcg
      661 gcctgggcgg catctggggc gtgcagacga tcgccttctt cccgttcgcg tatcagctca
      721 tcgccgacgt gctctcgcgg agcgacgcgc gcctcgagca ggccgcgcgc aacctcggcg
      781 ccggcccgtg gcaggtcttc cgatcggtga cgctgccgct gtcgcggccg ggcctcggag
      841 ccgcgatcct gacgaccgcg atctacgtgc tcgaggactt cggcaacccc gcgctcatcg
      901 gcggcaccta cacggtgctg ccgacgcagg cctacggtct catcagcggc ttcggcgacc
      961 ttcccggcgc gaccgccgtg agcacgctgc tgctcgcgct cgcgctggtg ctgtacttcg
     1021 cgaagatccg gctcgatggc ggccggtcct tcatcaccgt gagcgggcgc gcgtcttcga
     1081 tgccgcgccc gcccgtgccg gcggcgctca gctgggcgtg cttcgccgcg tgcgccgcgc
     1141 tctccatcgt catcatcacg gtctacgggt ccctggtcgt ctccgcgctc acgctgacgt
     1201 tcccgaccaa cctcggcttc acgacgaaac acttcgagta catcacctcg gggccgaacg
     1261 gcacagccct gcagaacacg ctcgtgttcg gtctcgtggc ggcggtcttc tcggcgctgt
     1321 tcgcgctcgt cgcgggatgg ctggtcgagc gcggcgcgtg gaagggccgc ggggtcctcg
     1381 acttcctgct gatcatgccc gccgcgatcc ccggcctctt cttcgggatc ggctacgcga
     1441 ccgccttcaa cgaggcgtgg ctggactggc tcgaccgtgg tgtgctcatc atcatctcga
     1501 tgatcttctg gaacatcccc gtggggtacc aggcggcggt cgccgggctg cgccagatcg
     1561 accgatcgat ggacgaggcg gcgacgagcc tgggcgcctc gagcctgcgc gggttccgcg
     1621 acgtgctcct gccgatgctc ggcggatcgc tgcgtgtcgg cttcgtcacg accttcgtgc
     1681 gcgcggtgac gacgctctcg gtggtcatct tcctcttcac gccgtcgtcg accgtcgcca
     1741 ccatccgcat cttccagctc gtgaacgatc tcaactgggg tgccgcgacc gcgttcaccg
     1801 tcgcggacat cggcatggcc atcgtcgcgc tcagcgtgat ctcgctgctc gcgcgcgggc
     1861 gcatcgcgct gggggccgcc cgtgcgtgag tctcccgcga aggccggcga gatccacctg
     1921 cgcctggcgg gcgtgacgaa gcgcttcggc gagacggtcg cggtcgacga cgtgtcgttc
     1981 gagatcccgc gcggctcctt cgcgacgctc ctcggcccgt cggggtgcgg aaagacgacg
     2041 acgctgcgca tgatcgccgg cttctacgac cccgacgccg gcgacatcat cctgggcgga
     2101 cggcgcatca acgagctgcc ggcgcaccgg cgtggcacgg cgatggtctt ccaggactac
     2161 gcgctcttcc cgcacatgac cgtgcgtgac aacgtcgggt acgggctgcg tgtcgcgaac
     2221 atcgcgaagg acgagcgcga gcggcgcgtg aacgagaccc tcgagttcgt cggcctcggc
     2281 aagctcggtg accgctggcc caatcagctc tccggcggac agcagcagcg cgtcgccgtg
     2341 ggacgcgcgc tcgtgctgcg cccggagatc ctgctgctcg acgagccgct ctccaacctc
     2401 gacgcgaagc tgcgcgtcca gctgcggtgg gagctgcgcg cgctgcagca gcagctgggc
     2461 atgaccttcg tctacgtcac gcacgaccag gacgaggcgc tgtcgctctc ggactggatc
     2521 gcggtgatga acgccggaaa ggtcgagcag cacggcacgc cctgggagat ctactaccac
     2581 ccgcgcaccg ccttcctcgc cgacttcgtc ggcgcggtga acctggtgcc gggcagcgtg
     2641 cgcgagctgc agggaagcag cgcggtcgtg tcgttcggca agcgcgcgct gaccgtgcgc
     2701 gtaccggccg gtctgacggt caccccgggg agcggggtga agctctgcat ccgtccggaa
     2761 gccctcgcgc tcgcgccgtc cgcggcgggc gacggcgagg cgttccccgg ccgcgtcgcg
     2821 cgcaaggcgt tcctcggcga cctgatgcgc tactgggtgg tcgtcgacgg gcaggaatgg
     2881 atcgtggacc agccggatcc cggcgcgacg agcccgttcg agggcgacgt gctggtgcgg
     2941 gtgcggccgg agcgcgtgca cctcatcgcc ggataggtcc gcggtcagtc aagctcgagc
     3001 gtcttcccgt cggtggcgta gtcgagctcg agctccgcga gatgggccag cgtcgaggtg
     3061 ccgaggtgcg tgagcacgat gcggcggcag tcgaggtcgg cgcggtgctt catcagcgtt
     3121 cgatacgaaa ggtgcgacgg atcgtccgtg tcgaagttcg tcgcctcgca gatgaagaga
     3181 tccgcgcccc gcgccacctc gggcaggcgg tcggtccagg tggcgtcacc ggagtacgcg
     3241 acgaggcggc ccgcgacgcg cacccgcagc ccgtgcgggt ccgagagcgg gacgtgatcg
     3301 accggcagcg cggagacgcg tgcgccgccg aggtcgatct cgtccgcacc gagcggcacg
     3361 acacggacct cgaagcccag cctggtgccg ccgaagaagt cggggtagag gccctgctcc
     3421 gacgcgtgca ggcgcgcggc gagctcccgc ggcccgccga tcgtcagcgg ccgggtgcgt
     3481 cccgcgaagt gctgctggat gacgaagtag gggatgccgc cgaagtgatc gccgtgcagg
     3541 tgcgagacgg cgatcgcgtc gatgccgtcg gcgaggccgg cacgcgtgat cgccggcagc
     3601 gcggagccgc cgcaatcgag cagcagcgac acgccggggg ccgtgacatg gacgcacgcg
     3661 tgcgaccggc cgccgtcggc gaacgcgtta ccggatccga ggaagcggac gccgatggtc
     3721 acgcgatctc ggacaccttc accgtccggc gctcgcgcca cgaccggccc gccgcctcgg
     3781 cgatccgcag cgcctccagc gcgtccgccg ccgtgcatgc gttcggcgcg tcccctcgcg
     3841 cgaagcggag gaagtgcgcg agctcggcgc ggtacgcgtc ggcgaagcgg acgaagaacg
     3901 acggatacgc cggtccggcg agcgccggcg cgtccgcctc gaccgaccgc cagggcgtgc
     3961 gcgggtcgat gccgatggcg agcgtgtcct tcgcggcgtg cacctcgacg tggatgtcgt
     4021 accccgcctc gttgtgccgc aggccggtga tgagcccgag cgacccgccg cgcatcttca
     4081 cgagcaccgc ggacgtcgcc acgtcgcgca tgccctcgta ctgcggcagg ccgagcgtcg
     4141 atcccgtcgc cgtgacctcc tcgacctcgt cgtcgaagag ccaccgcgtg atgtcgaagt
     4201 cgtggatcag ctggtccttg aactgtccgc cggaggtcgc gatgtacgcg tcgggcggcg
     4261 ggagccggtc gcagctcacc atgacgaagg agtacaccgg gccgaggcgc ccgctcgaga
     4321 gcgcgtcctt ggcgctgccg tagccggcgt cgaagcgccg ctggaacccg atctggagcc
     4381 ggccgttcgt gcgctcggca tgcgcgacga cgccgcgcgt ctcgtcgatg ccgatggcga
     4441 tcggcttctc gcagaacgtc gcgatgcgcg cgtccaggca gcgcctgatg agcggcgcgt
     4501 gcgtgtcggt gggggtgacg atgaccaccg cgtccgcgcc gtccagcgcc gcgtcgatcg
     4561 tatcgacggc gcggccgccc accttccccg cgacctcgtt cgcgcgggcc gcgtccgcgt
     4621 cgtagatcgc gaggtccgct acgtcgggca gcgcgcgcag cgtggccgcg tgcgcggcgc
     4681 ccagccggcc gacgcccagc agaccgatcc tcatcctgac cctcccgata cgtctcggcg
     4741 ctggacgcta gcgtacggtc atgcagcttg ccgccgcgcc gatctcatgg ggcgtctgcg
     4801 aggtgccggg ctggggatac cagttgccgc cggaccgcgt gctcgccgac atgcgccggc
     4861 tcggtctgcg cgacatcgag gccggaccac cgggcttcct cccggccgac gacgccgcgg
     4921 cgcgcgcgct cgtcgcgcgc ctggacctgc gcgtcgtcgc ggcgttcgtc acggcggtgc
     4981 tccatcgacg cgacgtcttg g
//
LOCUS       DASTZR010000020         2938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1098018, whole genome shotgun sequence.
ACCESSION   DASTZR010000020 DASTZR010000000
VERSION     DASTZR010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098018"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1768)
                     /locus_tag="VFV20_00435"
     CDS             complement(<1..1768)
                     /locus_tag="VFV20_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5286845.1"
                     /translation="MLRSVRDQYLPPDHDAFVAAEPPTGRVIVIAPTRAACETIELAL
                     GLHLDTFLEREHGAEVRELAASGKGFGIVAGTGTGKTLAVRPISEIILKAPLKVGVVN
                     REREATPDTPTWNVIIVTTGIARRWFQDGDILASDTLVVDEIHQTSAELELCLALGKR
                     VGCRFIWLSATVDPSFYASYLESSGVVQSFAFDPAKAAKVTVVDKEPIEFLDDRFLQR
                     VLKERRGVGVFLPTRNAVEEAAQQVEERYPRINTAFYHGGEPIRIIRPFLEEDNQKPF
                     LLAMTAAGQSALNVRGLDTVVIDDSRFANVIERGRNVLTRVHLGANEILQMAGRVHGR
                     VENGRVFILSDRNIVFSELRPTAPDFQLAGDSERVAITCADLGVRADELELPVPLDKQ
                     SYRKALALLEDRGIVENGRLTRYGKLVEAMPVDRPWAELLVHADDELVPYVAVMSSVE
                     SLHRMTRDDRNLEGLVVPGSDHLTAYNLYAEGFTRCGYIGQVYGLPRHLFDDTVEQWA
                     EQRGVLVKAIEDAALGMASVYRALNLELPSKMVLARDHAYGKFVELLAEVMPFDLVID
                     EETASGDSARVSKTSVCGSWGPI"
     gene            complement(1927..2667)
                     /locus_tag="VFV20_00440"
     CDS             complement(1927..2667)
                     /locus_tag="VFV20_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286846.1"
                     /translation="MKKIALTLIAAGAVFATEASAQVMVRASSVPPGQRPPAGMCRVW
                     IEGVPPGRQPAVTDCATARATAPLNSRIIFGSDAPFPGRATGRFRDGNCEFERRSSNL
                     GDIFFGLRTSRRDSDRDCRVSSSRTRDLGVWYPVGRDSNGNVIYERRTRDRSGRIITE
                     RARSRDGRTLSRIGSNRDIRRARDRDDDDDEDDDRRMTRVERERDRAIRDREKAIRKQ
                     QKAIEKARRDREKAARKAGRGNRSGRDS"
     gene            complement(2692..>2938)
                     /locus_tag="VFV20_00445"
     CDS             complement(2692..>2938)
                     /locus_tag="VFV20_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286847.1"
                     /translation="PSVPRRERDEQPRIPSRYMPPAGMCRIWLEGVPASRQPAPTDCI
                     SALRNKPANGRVIFGDPLPSRKKGRSGNPFGIGSFEE"
BASE COUNT          456 a         1017 c          932 g          533 t
ORIGIN      
        1 cgatcggacc ccagcttccg cacacgcttg tcttggacac gcgcgcgctg tcgccgctcg
       61 cggtctcctc gtctatcacc agatcgaacg gcatcacttc ggcgagcagc tccacgaact
      121 tgccgtacgc gtgatcgcgc gcgagcacca tcttcgacgg cagctccagg ttcagcgcgc
      181 ggtacacgct cgccattccc agcgcggcgt cctcgatggc cttcaccagc acgccgcgct
      241 gctcggccca ctgctcgacg gtgtcgtcga acaggtgccg gggcagcccg tacacctggc
      301 cgatgtagcc gcagcgcgtg aatccctccg cgtacaggtt gtacgccgtg agatggtcgc
      361 tccccggcac caccagcccc tcgagattcc ggtcgtcgcg cgtcatgcgg tggagagatt
      421 ccaccgagct catcaccgcg acgtacggca ccagttcgtc gtcggcgtgc accagcagct
      481 ccgcccacgg gcgatccacc ggcatcgctt cgaccagctt gccatagcgc gtgaggcggc
      541 cgttctcgac gatcccccgg tcttcgagga gcgcgagcgc tttcctgtac gactgcttgt
      601 ccagcggcac cggcagctcg agctcgtcgg cgcgaacgcc gaggtcggcg cacgtgatcg
      661 ccacgcgctc ggagtcgccc gcgagctgga agtcaggcgc ggtcgggcgc agctcggaga
      721 agacgatgtt gcggtcggag agaatgaaaa cgcggccgtt ctcgacccgg ccgtgcacgc
      781 gtcccgccat ctgcaaaatc tcgttggcgc cgaggtgcac gcgcgtgagc acgttgcgtc
      841 cgcgctcgat cacgttggcg aagcgtgagt cgtcgatcac gacggtgtcg agcccgcgca
      901 cgttgagcgc gctctgcccc gccgccgtca tcgcgaggag gaacggcttc tggttgtcct
      961 cctcgaggaa gggccggatg atgcggatcg gctcgccgcc gtgatagaag gccgtgttga
     1021 tgcgagggta ccgctcctcg acctgctgcg ccgcttcctc taccgcgttc cgcgtcggca
     1081 ggaacactcc cactccgcgt ctttccttga gcactcgctg cagaaaccga tcgtcgagaa
     1141 actcgatcgg ctccttgtcc accacggtca ccttggccgc cttcgccgga tcgaacgcga
     1201 agctctgcac gactccggag gactcgaggt agctcgcgta aaacgacggg tccaccgtcg
     1261 ccgacagcca gatgaaccgg caccccacgc gcttgccgag cgcgaggcac agctccagct
     1321 cggcggaagt ctggtggatc tcgtccacga cgagcgtgtc gctcgcgaga atgtcgccgt
     1381 cctggaacca ccggcgcgcg atgccggtgg tgacgatgat cacgttccac gtcggcgtat
     1441 cgggcgttgc ctcgcgctcg cggtttacga ctccgacttt gagcggcgcc ttgaggatga
     1501 tctcggaaat ggggcggacg gcgagggttt tcccggtccc cgtccccgcc acgatcccga
     1561 acccctttcc cgaagccgcg agctcgcgaa cctcggcccc gtgctcgcgc tcgaggaaag
     1621 tgtcgaggtg caggccgagc gcgagctcga tagtctcgca tgcggcgcgg gtgggtgcga
     1681 tgacgatgac gcggccggtg gggggctccg ccgcgacgaa ggcgtcgtgg tcgggcggga
     1741 gatactggtc gcgtacgctg cggagcatga gggaagatag tccgatcgtg ttgccatgga
     1801 atccaagagg tgagatgcca gatacgagaa gccagatgcc cagaaaaaaa tctggacatc
     1861 tggcttctta tctctggctt ctcacttctt ggattcccct tcagcccgct cggagcgtgc
     1921 tatcgtctag ctgtctcgtc cgctccgatt gcctctcccc gctttgcgtg cagccttctc
     1981 acgatcccgg cgcgccttct cgatcgcctt ctgctgcttg cggatcgcct tttcccggtc
     2041 gcggatcgcc cgatcccgct cgcgctcgac ccgcgtcatc cggcgatcat cgtcctcatc
     2101 gtcatcgtcg tcacgatccc gagccctccg gatgtcgcgg ttcgatccga tccgcgaaag
     2161 cgtacggcca tcacggctcc gcgcacgctc ggtgatgatc cgtccgctgc gatcgcgcgt
     2221 tcgacgctcg tagatcacgt tgccgttcga atcacgcccg accggatacc agactccgag
     2281 gtcacgcgtg cggctcgagc tcacgcggca gtcgcgatcg ctgtcgcgcc gcgaggtgcg
     2341 caggccaaag aaaatgtcgc cgaggttcga gctgcgtcgc tcgaactcgc agttaccgtc
     2401 gcggaaacga ccggtcgctc gtccggggaa cggcgcgtcg ctgccgaaaa tgatccgcga
     2461 attgagcggc gccgttgccc gcgccgtcgc gcaatcggtg acggccggct ggcgtccggg
     2521 cggcacgcct tcgatccaga cgcgacacat gcccgcgggc ggacgctgtc cgggcggcac
     2581 gctgctggcg cgaaccatca cctgcgcgct cgcttccgtc gcgaacaccg cgccggcggc
     2641 gatcagtgta agtgcgatct ttttcatcgg tccctccggt ttagctgagt gctattcctc
     2701 gaaggagccg atcccgaacg gattgccgct cctgcccttt ttcctcgaag ggagcggatc
     2761 gccaaagatc acacggccgt tcgccggctt gttgcgcagc gccgagatgc aatcggtcgg
     2821 cgcgggctga cggctggcgg gtacgccctc gagccagatg cggcacatcc ccgctggggg
     2881 catgtaccgg ctcgggatcc gtggctgctc atctctctca cgccgcggca ccgacggc
//
LOCUS       DASTZR010000021         2872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_993716, whole genome shotgun sequence.
ACCESSION   DASTZR010000021 DASTZR010000000
VERSION     DASTZR010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993716"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /locus_tag="VFV20_00450"
     CDS             <1..1360
                     /locus_tag="VFV20_00450"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5286848.1"
                     /translation="DVRDLVRVTEARATSIERERAEIVERRERLASERASASTVRGHA
                     ADAFAVAERGESGANDAHAETTRRAEAARLDSATSEERRSSLVRLRDTLDEQVSATEK
                     RIAEDEERLRALAAQEREGRARMEAAQADLGRATAEAHEAERAAELARQRALAAEGSR
                     RESEQRLAKARERLAELRGERGKLAVEEERASGALRLLEEQVRAELGLPDDEPLPDPT
                     TLELDDEPDEKEKASTLGALQRLRRRLIALEPVNPLAATELAEIGQRHEFLTTQQADL
                     EKAMADLRTLADELAMTIAEQFSTTLMAVDREFGVFFQRLFNGGQASLRTSEDPDEPG
                     IDIYARPPGKRIGSLAQLSGGERALTATALLLAILRVKPAPFCVLDEVDAALDERNVG
                     RFTQALKELTDRTQFIVITHNRGTIEAADTIYGVSMDDGGVSKVLSLKLSELDEQRLR
                     VG"
     gene            1368..1742
                     /locus_tag="VFV20_00455"
     CDS             1368..1742
                     /locus_tag="VFV20_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286849.1"
                     /translation="MTWRRIALIIAWVAAAGWGIYYLAALASGQAGFTAAAVAHFVFG
                     YAWAHAIVARPLTMPLALFGTVCAGAFTRVALGMGTAEIYVAWIGPLAALALADTTRP
                     RYIAYSIGAFALGFLLLIWLTA"
     gene            1774..2679
                     /gene="ftsY"
                     /locus_tag="VFV20_00460"
     CDS             1774..2679
                     /gene="ftsY"
                     /locus_tag="VFV20_00460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEU5286850.1"
                     /translation="MSMQQGLAKTRGGFLSRLFGRKQEEPATPEFLDELEEGLLRADL
                     SVSLVDPLMAQVRDLVSTGEVKTVPKVLDAVRDVLVRELAGTDASLRTTSARPRVILV
                     VGVNGSGKTTTAAKLAKRLKDAGETPLLAAADTFRAAAIEQLETWASRLDIPVVSGRP
                     DGDPAAVVFDAVSAAAARGNSTVIADTAGRLHTKGHLMDELGKIVRVTGRAREGAPDE
                     VLLVLDGTAGQNAIQQARQFLKDASVTGLVVTKLDGTAKGGAVFAIVHELGLPVKFLG
                     VGEKADDLLPMDARAFVDALLPRAG"
     gene            complement(2684..>2872)
                     /locus_tag="VFV20_00465"
     CDS             complement(2684..>2872)
                     /locus_tag="VFV20_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286851.1"
                     /translation="QLIPYSIAGGMGIYLGIGAWRELRRPTDAWLGLPKDRLRDVAFA
                     YVIIPPLFLVASLWEFFA"
BASE COUNT          429 a         1013 c         1065 g          365 t
ORIGIN      
        1 cgacgtccgc gacctcgtgc gcgtgaccga ggcgcgcgcg acctcgatcg agcgggagcg
       61 ggcggagatc gtcgagcgcc gcgagcggct cgcgtcggaa cgcgcctccg cctcgaccgt
      121 gcgcggacac gcggcggatg ccttcgcggt ggccgagcgc ggcgagagcg gcgcgaacga
      181 tgcccatgcc gagaccacgc ggcgcgccga ggcggcacgc ctcgactcgg cgaccagcga
      241 ggagcgccgc tcgtcgctgg tgcgcctccg cgacacgctc gacgagcagg tctccgcgac
      301 cgagaagcgg atcgccgagg acgaggagag gctgcgcgcc ctcgccgcgc aggagcgcga
      361 ggggcgtgcg cgcatggagg cggcgcaggc cgatctcggt cgcgcgaccg ccgaggcgca
      421 cgaggcggag cgtgccgcgg agctcgcgcg ccagcgcgcg ctcgccgcgg agggatcgcg
      481 ccgcgaatcc gagcagcgcc tggcgaaggc gcgcgagcgt ctggccgagc tgcggggcga
      541 gcgcggcaag ctcgccgtcg aagaggagcg cgcctccggt gccctgcgtc tgctcgaaga
      601 gcaggtccgc gcggagctcg ggcttccgga cgacgagccg ctgccggacc cgaccacgct
      661 cgagctggac gacgagccgg atgagaagga gaaggcgagc acgctcggcg cgctgcaacg
      721 cctgcgtcgc cggctcatcg cgctcgagcc ggtgaaccca ctcgccgcga ccgagctcgc
      781 ggagatcgga cagcgccacg agttcctcac gacacagcag gcggacctcg agaaggccat
      841 ggccgacctg cggacgctcg ccgacgagct cgcgatgacg atcgccgagc agttctcgac
      901 cacgctgatg gcggtcgacc gggagttcgg cgtcttcttc cagcgcctct tcaacggagg
      961 ccaggcgagc ctgcgcacct ccgaggatcc cgacgagccg ggcatcgaca tctacgcgcg
     1021 cccgccgggt aaacgcatcg gatcgctcgc gcagctgtcc ggcggcgagc gcgcgctcac
     1081 ggcgaccgcg ctgctcctcg cgatcctgcg cgtgaagccc gcgcccttct gcgtgctgga
     1141 cgaggtggac gccgcgctcg acgagcgcaa cgtcggccgc ttcacgcagg cgctgaagga
     1201 gctcaccgac cgcacgcagt tcatcgtcat cacgcacaac cgcggcacga tcgaggcggc
     1261 cgacacgatc tacggcgtgt cgatggacga cggcggcgtg tcgaaggtcc tgtcgctcaa
     1321 gctctccgag ctcgacgagc agcgcctgcg cgtcggatag cgtcgcggtg acctggcggc
     1381 gcatcgcgct catcatcgcg tgggtggccg cggcgggatg gggcatctac tacctcgccg
     1441 cgctcgcgag cggccaggcc ggtttcaccg cggcggccgt cgcgcacttc gtgttcggct
     1501 acgcgtgggc gcacgccatc gtcgcgcggc cgctcacgat gccgctcgcg ctcttcggca
     1561 cggtttgcgc gggcgcgttc acgcgcgtcg cgctcgggat gggcacggcg gagatctacg
     1621 tcgcatggat cggaccgctc gccgcgctcg cgctcgcaga caccacgcga ccgcgctaca
     1681 tcgcgtattc gatcggcgcg ttcgcgctcg gattcctgct gctcatctgg ctcacggcct
     1741 gatcacgtcc gcgcacggta ccgttgtcgg gcgatgagca tgcagcaggg tctcgcgaag
     1801 acgcgcggcg gattcctctc gcgtctcttc ggccgtaagc aggaggagcc cgcgacgccg
     1861 gagttcctcg acgagctcga ggaggggctg ctgcgcgcgg acctctccgt gtcgctcgtc
     1921 gacccgctca tggcgcaggt gcgcgacctg gtgtccaccg gcgaggtgaa gacggtgccg
     1981 aaggtgctcg acgcggtgcg ggacgtcctg gtgcgggagc tcgcgggcac ggacgcgagc
     2041 ctgcggacga cgagcgcccg gccgcgcgtg atcctcgtcg tcggtgtgaa cggcagcggc
     2101 aagacgacga cggccgcgaa gctcgcgaag cgcctgaagg acgccggcga gacgccgctc
     2161 ctcgcggcgg cggacacgtt ccgcgcggcg gcgatcgagc agctcgagac gtgggcgtcg
     2221 cgcctcgaca tcccggtggt ctcgggacgc ccggacggcg atccggcagc tgtcgtcttc
     2281 gacgccgtga gcgcggcggc ggcgcgcggc aacagcaccg tcatcgcgga cacggccgga
     2341 cggctgcaca cgaagggtca tctcatggac gagctcggca agatcgtgcg cgtgaccggc
     2401 cgcgcccgcg agggcgcgcc cgacgaggtg ctgctggtgc tcgacggcac cgccggccag
     2461 aacgcgatcc agcaggcacg gcagttcctc aaggacgcca gcgtgaccgg gctcgtggtc
     2521 accaagctcg acggcaccgc caagggcggc gcggtgttcg cgatcgtgca cgagctcgga
     2581 ctgccggtga agttcctcgg tgtgggagag aaggcggacg acctgctgcc gatggacgcg
     2641 cgcgcgttcg tcgacgcgct gctcccgcgc gcgggctgag cctctaggcg aagaactccc
     2701 acagcgacgc gaccaggaag agcggcggga tgatgacgta cgcgaacgcg acgtcgcgca
     2761 gccggtcctt cggcagcccc agccacgcgt ccgtcggccg ccgcagctcg cgccaggcgc
     2821 cgatgccaag gtagatgccc atcccgcccg cgatcgaata ggggatgagc tg
//
LOCUS       DASTZR010000022         2768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_993730, whole genome shotgun sequence.
ACCESSION   DASTZR010000022 DASTZR010000000
VERSION     DASTZR010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993730"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VFV20_00470"
     CDS             complement(<1..243)
                     /locus_tag="VFV20_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEU5286852.1"
                     /translation="MLGRERMVEWQGLLEPHHVIENIIRASTIYLLLFLLLRFLPNRK
                     TGSLGPTDLLVVVLLATAVHSALIREGSSLTDAAVLV"
     gene            301..564
                     /locus_tag="VFV20_00475"
     CDS             301..564
                     /locus_tag="VFV20_00475"
                     /inference="COORDINATES: protein motif:HMM:NF018574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HEU5286853.1"
                     /translation="MSPAKTRVTSHFLYVPDQDAAERAGRALARAGFHSEAGPASDGN
                     DWLLVATHDALAEKDRQVATQEAMREIALAVGGQYNGYAVRAK"
     gene            561..1532
                     /locus_tag="VFV20_00480"
     CDS             561..1532
                     /locus_tag="VFV20_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU5286854.1"
                     /translation="MSVLSGARVVVTGGAGNVGSHIVDGALAAGAREVVALDAMVRGV
                     PENLAGAIATGKARLVEIDIRDREAVRANLEGADVVFHQAALRWTRCQELPRECQEVM
                     VDGTFNVLEAAAEAKAKRVVLASSAVVYGEPVRLPIDEDQPLAGTTVYGAAKVANEQM
                     ARAFAHLRDLPTTTLRYFNVYGPRMDVRSQYVEVMVRWLNLIDERKPLTLFGDGSSSV
                     DFVYIGDVARANLLACETKATDAIVNVCSGVETRMRDLAALLLEVTGSSVGMEFKPQP
                     AGALPARRVGDPKRAQELLGFRAETSLADGVRKLIEWRRRVLAGSAA"
     gene            1544..2704
                     /locus_tag="VFV20_00485"
     CDS             1544..2704
                     /locus_tag="VFV20_00485"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016431015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU5286855.1"
                     /translation="MATETQKKLNVPITKPSLGEEEARAPFESIKSGWVTQGPKVAEF
                     EKAVASYVGAKHGVATTSCTTGLHLALTAAGIGKGDEVIVPSFTFIASANAILYTGAD
                     VVFCEVDPRTFNIDPNDVEKRITKRTKAIMPIDQIGLACDVGAINDIAKKHGLDVVED
                     AAPAIGEDYKGRKVGSNAHQTVFSFHPRKVITTGEGGMITTDDDALADRARKLRAHGM
                     TVSDLDRHKADRVIIEEYAELGFNYRMTDIQAAIGLVQIRRLDELLRIRREKAARYNR
                     ELAEVKGIEVPFVPAYAPHTYQSYCLRLTKDCRLDREDLMTNLLRRGIATRRGVMASH
                     LEKTYTSRYGKVSLPVTEEATRTTMLIPLYATMTDEEQDWVIQSLREELGTK"
BASE COUNT          486 a          913 c         1004 g          365 t
ORIGIN      
        1 gacaaggacc gcggcatcgg tcagcgagct gccttcgcgg atgagcgcac tgtgcaccgc
       61 tgtcgcgagc agtacgacga ccaggaggtc cgtcggcccg aggctcccgg tcttgcggtt
      121 cggcaggaac cgcagcagca ggaagagcaa gaggtagatg gtggacgcgc ggatgatgtt
      181 ctcgatgacg tggtgcggct cgagcaggcc ctgccattcg accatgcgct cccggccgag
      241 cacgacccgt gccgttgtgc gtacgctgtg cgtgatggcg cgtgctgcga gacggagcgc
      301 atgagtccgg ccaaaacgcg ggtcaccagc cacttcctgt acgtccccga ccaggacgcc
      361 gccgagcgcg ccggtagggc gctggcgcgc gcgggcttcc actccgaggc ggggccggcc
      421 tccgacggca acgactggct cctcgtggcc acgcacgacg ccctggctga gaaggaccgt
      481 caggtggcga cgcaggaggc gatgcgcgag atagcgcttg ccgtcggcgg tcagtacaac
      541 gggtacgcgg tgcgcgcgaa gtgagcgtgc tgtcgggcgc gcgcgtcgtg gtgacgggcg
      601 gagccgggaa cgtgggatcg cacatcgtcg acggcgcgct cgccgcgggc gcgcgcgagg
      661 tcgtggcgct cgacgccatg gtgcgaggcg tcccggagaa cctcgcgggc gcgatcgcga
      721 cggggaaggc gcgcctcgtc gagatcgaca tccgcgaccg cgaggcggtg cgggcgaacc
      781 tcgaaggggc cgacgtcgtg ttccaccagg cggcgctccg ctggacgcgc tgtcaggagc
      841 tcccgcgcga atgccaggag gtcatggtcg acggcacgtt caacgtgctc gaggccgccg
      901 cggaggcgaa ggcgaagcgc gtggtgctcg cgtcgagcgc ggtcgtctac ggcgagcccg
      961 tgcgcctgcc catcgacgag gatcagccgc tggcgggcac gacggtgtac ggcgcggcga
     1021 aggtcgcgaa cgagcagatg gcacgcgcct tcgcgcacct gcgcgatctc ccgacgacga
     1081 cgctgcgcta cttcaacgtc tacgggccgc gcatggacgt gcgctcgcag tacgtcgagg
     1141 tgatggtgcg ctggctgaac ctcatcgacg agcggaagcc cctcacgctc ttcggcgacg
     1201 gcagctcgtc ggtggacttc gtgtacatcg gcgatgtcgc gcgcgcgaat ctgctcgcgt
     1261 gcgagacgaa ggccacggac gctatcgtga acgtctgcag cggcgtggag acgcgcatgc
     1321 gcgacctcgc cgcactcctg ctggaggtca ccggcagcag cgtcgggatg gaattcaagc
     1381 cccagccggc cggcgcgctc ccggcgcggc gggtcggcga cccgaagcgc gcgcaggagc
     1441 tgctcggatt ccgagcggag acgagcctgg cagacggcgt gcggaagctg atcgaatggc
     1501 ggcggcgggt cctcgccggg tccgctgcct aaggagaaga cgaatggcga ccgagacaca
     1561 gaagaagctg aacgtcccga tcacgaagcc cagcctcggc gaggaggagg cgcgcgcccc
     1621 gttcgagtcg atcaagagcg gatgggtgac gcagggaccc aaggtcgccg agttcgagaa
     1681 ggccgtcgcc agctacgtcg gcgcgaagca cggcgtcgcg acgacctcct gcacgaccgg
     1741 tcttcacctc gcgctgaccg cggcggggat cgggaagggc gacgaggtca tcgtgccgtc
     1801 gttcaccttc atcgcctccg cgaacgcgat cctctacacg ggcgcggacg tcgtgttctg
     1861 cgaggtcgat ccgcggacgt tcaacatcga cccgaacgac gtcgagaagc gcatcaccaa
     1921 gcggacgaag gcgatcatgc cgatcgacca gatcggcctc gcctgcgacg tcggcgcgat
     1981 caacgacatc gcgaagaagc acggcctcga cgtcgtcgag gacgcggcac ccgcgatcgg
     2041 cgaggactat aaaggccgca aggtcggctc gaacgcgcac cagacggtct tctcgttcca
     2101 cccgcgcaag gtcatcacca cgggcgaggg cggcatgatc acgaccgacg acgacgcgct
     2161 ggccgatcgc gcgcgcaagc tacgcgcgca cggcatgacc gtgtcggacc tcgatcgcca
     2221 caaggcggac cgcgtgatca tcgaggagta cgccgagctc ggcttcaact accgcatgac
     2281 cgacatccag gcggccatcg gcctcgtgca gatccgacgg ctcgacgagc tgctgcgcat
     2341 ccgccgcgag aaggcggccc ggtacaaccg cgagctggcc gaggtgaagg gcatcgaggt
     2401 ccccttcgtg ccggcgtacg ccccgcacac gtaccagtcg tactgcctgc gcctcacgaa
     2461 ggactgccgc ctcgaccgcg aggacctcat gacgaacctg ctgcgccgcg gcatcgcgac
     2521 gcggcgcggc gtcatggcgt cgcacctcga gaagacgtac acgtcgcgct acgggaaggt
     2581 gagcctgccc gtcaccgagg aggcgacgcg cacgaccatg ctcatcccgc tctacgcgac
     2641 gatgaccgac gaggagcagg actgggtcat ccagtcgctg cgcgaggagc tcgggacgaa
     2701 gtagtcgcgg tggcaggggc gcagccccgg ggtgaagggg agcagcccct gcgatgaggt
     2761 agacaggc
//
LOCUS       DASTZR010000023         3032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_392849, whole genome shotgun sequence.
ACCESSION   DASTZR010000023 DASTZR010000000
VERSION     DASTZR010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392849"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VFV20_00490"
     CDS             complement(<1..673)
                     /locus_tag="VFV20_00490"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015920276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU5286856.1"
                     /translation="MTELERTDPEIYQAIENEKRRQIEKVELIASENYTSAAVMEAVG
                     SVLTNKYAEGYPGHRYYGGCEWVDVAESLAIERAKSLFGAEFANVQPHSGATANAAVL
                     HAIARPGDTLLGLALDQGGHLTHGMKINFSGRLYTIIHYGVSRETNLVDHDDVLALAK
                     EHRPKLIVCGGSAYPRTVETLRFREIADEVGALLLCDMAHFAGLVAAGLHPNPVEQCD
                     FVTSTT"
     gene            complement(670..1158)
                     /locus_tag="VFV20_00495"
     CDS             complement(670..1158)
                     /locus_tag="VFV20_00495"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEU5286857.1"
                     /translation="MKVGLAADHAGQTLKRSLEEHLRRTGHEVRLYGPPPDPEDDYPL
                     IARVLAEALVRGEFERAIFCCGSGIGPAIAANKVAGVRATVAENEWSARDGVSHVDVN
                     FLTLGERVIGDDLAKAIVDAYLGAKTEGGRHERRRKQIEAMEPRPMTTADAGQGMRRG
                     VK"
     gene            complement(1158..1757)
                     /locus_tag="VFV20_00500"
     CDS             complement(1158..1757)
                     /locus_tag="VFV20_00500"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEU5286858.1"
                     /translation="MKTRLLQVDTPGAVEEAAAALNRGDLVAFPTDTVYGLAAGHDHI
                     RKLYVAKDRPKDKRIPVLLSDATNLEASAFVTPAARALAAAFWPGPLTLVLVAPRRGT
                     IAFRVPDHALARRLIAASGGGLPVTSANRSGQPDARTAEEVVAQLEGRVALVLDGGRT
                     TGGVASTVVDCTTDRIKIIREGAVSVAEIERALRRAKVA"
     gene            1824..2441
                     /locus_tag="VFV20_00505"
     CDS             1824..2441
                     /locus_tag="VFV20_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286859.1"
                     /translation="MGRILGTLSHRDPLRRSLGAVGIALVLWFGAWVASYLFLAEGLL
                     RDLMSARIPDGILQAGPGLGVTIFEWNLLFGVGAVIVGSFFALGPISLGYFAPWWWAL
                     GYGVALGTNSFVITVPGKIAPRIDVLWNHIGAPEMLAYLLVAAALAHIHMWRPRRWYD
                     LKLARVRQIADIRLRASELGFVGGAIVLLFWTAHIEAAGVIAFLN"
     gene            complement(2422..>3032)
                     /locus_tag="VFV20_00510"
     CDS             complement(2422..>3032)
                     /locus_tag="VFV20_00510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEU5286860.1"
                     /translation="EFYGQRFRVDRRVLIPRPETETLVDEARRFIGGRALTVVDVGTG
                     SGAIAAHEHGVRVIGTDSSRDALAVAETNALALGVADRVEFRAGDLLALIAERVEVVC
                     ANLPYLRDDALAQLSGERTSLAFEPRAAVVAGGDGLDVIRRCVVDLSRVLAPGGAALF
                     ECDPPQAEAIAALLRQRGLVTRIVRDLSGAERVVVGSSSGTR"
BASE COUNT          403 a         1075 c         1060 g          494 t
ORIGIN      
        1 gggtggtcga ggtgacgaag tcgcactgct cgaccgggtt cgggtgcagc ccggctgcca
       61 cgaggccggc gaagtgggcc atgtcgcaga ggagcagcgc gccgacctcg tcggcgatct
      121 cgcgaaacct caaggtctcg acggtacgtg ggtacgcaga gcctccgcac acgatgagct
      181 tcggccgatg ctccttcgcg agtgcgagga cgtcgtcgtg gtcgacgagg ttcgtctccc
      241 gcgaaacgcc gtagtggatg atcgtgtaca gccggcccga gaagttgatc ttcatgccgt
      301 gcgtgaggtg gccgccctgg tcgagcgcga gacccaggag ggtgtcgccg gggcgggcta
      361 tcgcgtgcag gacggcggcg ttggccgtgg cgccggagtg cggctggacg ttggcgaact
      421 cggcgccgaa gagcgacttc gcgcgctcga tggcgagcga ctccgcgacg tccacccact
      481 cgcagccgcc gtagtagcga tgtcccgggt agccctcggc gtacttgttc gtgaggacgc
      541 tgcccacagc ctccatgacc gcggcgctgg tgtagttctc gctggcgatg agctcgacct
      601 tctcgatctg gcggcgcttc tcgttctcga tggcctgata gatctccgga tcggtccgtt
      661 cgagctccgt catttcacac ctctccgcat tccctggccg gcatccgccg tggtcatcgg
      721 tcgcggctcc atcgcctcga tctgtttccg gcgtcgctca tgacgcccgc cctcggtctt
      781 cgcgccgaga taggcgtcga cgatcgcctt cgcgaggtcg tcgccgatca cccgctcgcc
      841 cagtgtgagg aagttcacgt cgacgtgcga cacgccgtcg cgcgccgacc actcgttctc
      901 ggcgaccgtg gcgcgcacgc ctgcgacctt gttcgcggcg atcgccgggc cgatgcccga
      961 gccgcagcag aagatcgcgc gttcgaactc gccgcgcacg agcgcctccg cgaggacgcg
     1021 cgcgatgagc gggtagtcgt cctcgggatc gggcggcggg ccatacagcc tcacctcgtg
     1081 ccccgttcga cgcagatgct cctcgagcga acgcttcagc gtctggcccg cgtgatcggc
     1141 cgcgagcccg accttcatca ggccaccttc gcccggcgca gtgcgcgctc gatctcggcg
     1201 acgctcaccg caccctcgcg gatgatcttg atccggtccg tcgtgcagtc gacgacggtc
     1261 gaggcgacgc cgccggtcgt cctgccgccg tcgaggacga gcgccacccg gccctcgagc
     1321 tgcgcgacca cctcttcggc ggtgcgtgcg tcgggctgtc ccgagcggtt cgcgctcgtg
     1381 accggaaggc cgccgccgct cgcggcgatg aggcggcgtg cgagcgcatg gtcggggacc
     1441 cggaacgcga tggtcccgcg tcgcggcgcg acgagcacga gcgtgagcgg cccgggccag
     1501 aacgccgcgg cgagcgcccg cgcggcaggc gtgacgaacg cgctcgcctc gaggttggtg
     1561 gcgtccgaga gcaggaccgg gatgcgcttg tccttcggac ggtccttggc gacgtagagc
     1621 ttccggatgt ggtcgtgtcc ggcggcgagt ccgtacacgg tgtccgtggg gaaggcgacg
     1681 aggtcgccgc ggttcagggc ggcggccgcc tcctccaccg ccccaggtgt atcgacctgg
     1741 aggaggcgtg tcttcatggg ccgattctag gtcgttcgac cggtagcaac cgccgtcccg
     1801 cttcgttcca tgttccggca ggtatgggga ggatcctcgg cacgctcagt catcgcgacc
     1861 cgctgcgccg ctcgctcggc gcggtcggga tcgcgctcgt cctctggttc ggcgcgtggg
     1921 tggcgagcta cctcttcctc gctgagggcc tgctccgcga cctgatgtcg gcccgcatcc
     1981 cggacggcat cctccaggcc gggccgggcc tcggggtcac gatcttcgag tggaatctgc
     2041 tcttcggggt cggcgccgtg atcgtcggga gcttcttcgc cctcggaccg atctcgctcg
     2101 ggtacttcgc cccgtggtgg tgggcgctcg gctacggcgt cgcgctcggc accaactcgt
     2161 tcgtcatcac cgttcccggc aagatcgcgc cccgcatcga cgtcctctgg aaccacatcg
     2221 gcgcgccgga gatgctcgcg tacctcctcg tcgcggcggc gctcgcgcac atccacatgt
     2281 ggcgaccccg gcgctggtat gacctcaagc tcgcgcgtgt gcgtcagatc gccgatatcc
     2341 gcctgcgcgc gagcgagctg ggcttcgtcg gtggcgcgat cgtgctcctc ttctggacgg
     2401 cgcacatcga ggccgcgggc gtcatcgcgt tcctgaacta gaccccacca cgacccgttc
     2461 cgcaccggag aggtcacgca cgatgcgcgt gacgaggccc cgctgccgca ggagcgccgc
     2521 gatcgcctcg gcctgcggcg gatcgcactc gaacagtgcc gcgccgcccg gcgcgagcac
     2581 ccgggacaga tcgacgacgc agcgtcggat gacatcgagc ccatcgccgc ccgcgaccac
     2641 cgcggcgcgc ggctcgaacg cgagcgaggt ccgctcaccg gagagctgag cgagcgcgtc
     2701 gtcgcgcagg tacggcaggt tcgcgcagac cacctcgacg cgctcggcga tgagggcgag
     2761 caggtcgccg gcgcggaact cgacgcggtc ggcgacgccg agcgcgagcg cgttcgtctc
     2821 cgccacggcc agcgcgtcac gcgaggagtc ggtcccgatg acgcgcacgc cgtgttcgtg
     2881 cgccgcgatc gcgcccgatc ccgtcccgac gtcgacgacc gtgagcgcac ggccgccgat
     2941 gaagcggcgc gcctcgtcca cgagggtctc ggtctcgggc cgcgggatga gcacgcgccg
     3001 atccacgcgg aaacgctgcc cgtagaactc ct
//
LOCUS       DASTZR010000024         3014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_471594, whole genome shotgun sequence.
ACCESSION   DASTZR010000024 DASTZR010000000
VERSION     DASTZR010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471594"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VFV20_00515"
     CDS             <1..226
                     /locus_tag="VFV20_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018240098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEU5286861.1"
                     /translation="RRIGPGAGFGELALLLGRPRSATVTAVTELALASLGRAEFAWLV
                     RKSGETMGELRARTAHYVGDVGLGTAVRGA"
     gene            231..446
                     /locus_tag="VFV20_00520"
     CDS             231..446
                     /locus_tag="VFV20_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020512244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286862.1"
                     /translation="MDCWVCERTALGACRFCGRGTCREHARTHPFVLDVFRGTKHQTL
                     RALVVEDALHCGICKPRGEPVELPELE"
     gene            601..1233
                     /locus_tag="VFV20_00525"
     CDS             601..1233
                     /locus_tag="VFV20_00525"
                     /inference="COORDINATES: protein motif:HMM:NF038399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Os1348 family NHLP clan protein"
                     /protein_id="HEU5286863.1"
                     /translation="MSRDALAKVIQRSISDGAFRRQLATDPTGALRGYDLSGDEFAAI
                     KSGDSTRLTALGVEQRMSKAFTMSGDASGADSVSKAVTSDLGASWHGALTPTEGTTGN
                     DALTPVDGASEHVLTPGDGATYNGALISGDGDDADAVIADVSGSAGNAAITGGDTAEA
                     LPDDIDPGFVPQFNVLTSGDTDAQSIIVSDDAAGGGSTPGEAIDGPNIQE"
     gene            complement(1318..1992)
                     /locus_tag="VFV20_00530"
     CDS             complement(1318..1992)
                     /locus_tag="VFV20_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU5286864.1"
                     /translation="MDAAAIADYLQFSPVFAPLSVGERADLSARMRPRHFAKNEVVFH
                     KDDPAAHVYLIAAGTVKISIEDESGQEVVIALMRGGDVFGDLALFDQGERSATATALT
                     ETTMFALANRDFMDVLQHNAAAMRQLLALLARRIRHSTTHIEDLVFLDLPGRVAKCLI
                     DQNELLGGGGVVRLTQEDVANFVGATRVAVNRVLVDLERRKLLRLGRGSVTIADLEAL
                     KHEVRR"
     gene            complement(2027..>3014)
                     /locus_tag="VFV20_00535"
     CDS             complement(2027..>3014)
                     /locus_tag="VFV20_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015100418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU5286865.1"
                     /translation="TRGEDVRRGTALAAGFKNVCYSHGFDDFSTARVRLVRERGEPVA
                     YVLTAAAEVGQGIVAVCEQIARTALSVQRVVVETPDTSIGSAGSSSASRQTWMTGGAV
                     LEACREVRAELARRATLAGRGTLSPNGDVEVAALLDEPIERLLTHRHRDTEPRDATTQ
                     NRGHVAFLFAAHRATVDVDVETGLVRVVQVATSQDVGRAINPAAVIGQLEGGIAQGLG
                     LAVMEEVQLRDGLVRNASFTDYLLPTILDMPPVLCDLVEHPHPDAPYGAKGVGEPPTI
                     SSTPAIVAAIRAATGRELNRVPVRPDDIVFGTVARSGWRIVPGADPRAAVRV"
BASE COUNT          469 a         1049 c         1084 g          412 t
ORIGIN      
        1 gcgccgcatc ggccccggcg ccggtttcgg cgagcttgcg ctgctcctcg gccggccacg
       61 gtcggcgacg gtcaccgccg taacagagct ggcgctcgcc agcctggggc gggcggagtt
      121 cgcctggctg gtgcggaagt ccggggagac gatgggcgaa cttcgggcgc ggacggcgca
      181 ctatgtggga gacgtcgggc tcggcacggc agtgaggggg gcgtagcgaa gtggactgct
      241 gggtctgcga gcgcaccgcg ctgggcgcct gccgcttctg cgggagaggc acatgccgcg
      301 agcacgcgcg gacgcatccg ttcgtgctcg acgtcttccg cggtacgaag caccagacgc
      361 tgcgcgcact cgtcgtcgag gacgcgctgc actgcggcat ctgcaagccg cggggcgagc
      421 cggtggagct gcccgagctc gagtaggcgg aaggagggga cggcaggggc gggacgcgct
      481 cgcgcggccg ctcctattgt gatcaggatc acagaggaca tgacacgggg atggacgagc
      541 cgaacgcgtg acagggccag tgttccgcaa aacgcagcca ggcgatagga ggcagacgac
      601 atgtcaaggg acgctcttgc aaaggtcatc cagcgctcga tcagcgacgg tgctttccgc
      661 cgtcagctcg cgaccgatcc gacaggcgcg ctccgcggct acgatctgag cggagacgag
      721 ttcgccgcca tcaagagcgg cgattccacg cggctcaccg cgctcggcgt cgagcagcgc
      781 atgagcaagg ccttcacgat gtccggcgac gcgtccgggg cggactccgt gtcgaaggcc
      841 gtgaccagcg acctcggtgc gagctggcac ggcgcgctca cgccgaccga gggaaccacg
      901 ggcaacgacg ctctcacacc ggtcgacggc gcatcggaac acgtgctcac gcccggcgac
      961 ggagcgacgt acaacggggc actgatcagt ggcgacggcg acgacgccga tgcggtgatc
     1021 gcggacgttt cgggctccgc cggcaacgcg gcgatcacgg gtggggacac cgccgaggcg
     1081 ctccccgacg acatcgaccc gggtttcgtc ccccagttca acgtcctgac gtcgggcgac
     1141 accgatgccc agagcatcat cgtgagcgac gacgccgcgg gcggcgggtc gacgccgggc
     1201 gaggccatcg acggcccgaa catccaggag taaccacggc ctcgcgcgtc tggcgcgatt
     1261 accctctcgc ggtccgaacg agcggcctcc ccaagaggcc gctcgttcac tgtgcgttta
     1321 gcgcctgacc tcgtgcttga gggcctctag gtcggcgatc gtcacggacc cgcggcccag
     1381 ccgcagcaac ttgcgccgct cgaggtcgac gagcacgcgg ttcacagcga cgcgcgtcgc
     1441 gccgacgaag ttcgcgacgt cctcctgggt gagccgcacc acgccgcccc cgccgagcag
     1501 ctcgttctgg tcgatgaggc acttcgccac gcggccgggg aggtcgagga acacgagatc
     1561 ctcgatgtgg gtggtgctgt ggcggatccg ccgggccagc agcgcgagca gctggcgcat
     1621 cgccgcggcg ttgtgctgca ggacatccat gaagtcacga ttcgcgagcg cgaacatcgt
     1681 ggtctccgtg agtgcggtcg cggtcgcgga gcgctcgccc tgatcgaaca gcgccaggtc
     1741 cccgaagacg tcgccgccgc gcatcagcgc gatgacgacc tcctggccgc tctcgtcctc
     1801 gatcgagatc ttcaccgtgc cggcagcgat gaggtagacg tgggccgccg ggtcgtcctt
     1861 gtgaaagacg acctcgttct tcgcgaaatg gcgcgggcgc atgcgcgccg aaaggtcggc
     1921 gcgctccccg accgagagcg gcgcgaacac cggcgagaac tggaggtagt cggcgatcgc
     1981 cgccgcgtcc acgggcagag tatgaggcgc gcgctccggg aggaagctac acgcgcaccg
     2041 ctgcgcgcgg atcggcaccc ggcacgatcc gccagccgga ccgcgcgacg gtgccgaaca
     2101 cgatgtcgtc ggggcggacc gggacgcggt tcagctcgcg gcccgtcgcc gcgcggatcg
     2161 cggccacgat cgccggcgtg ctcgagatcg tgggcggctc ccccacgccc ttcgcgccgt
     2221 acggcgcgtc gggatgcgga tgctcgacga ggtcgcagag gaccggcggc atgtcgagga
     2281 tggtcggcag caggtagtcc gtgaacgacg cgttgcgcac gaggccatcc ctgagctgca
     2341 cctcctccat gaccgcgagc ccgagcccct gcgcgatgcc gccctcgagc tgaccgatca
     2401 cggcagcggg gttgatggcc ctgcccacgt cctgcgatgt cgcgacctgg accaccctca
     2461 ccaggccggt ctcgacgtcg acgtcgaccg tcgcgcggtg cgcggcgaag aggaacgcga
     2521 cgtgcccgcg gttctgggtc gtcgcgtcgc gcggttcggt gtcgcgatga cgatgtgtga
     2581 gcagccgctc gatgggttcg tcgagcaggg ccgccacctc cacgtcgccg ttcggcgaga
     2641 gggtcccccg tccagcgagc gttgcgcgcc gcgcgagctc ggcacgcacc tcgcggcacg
     2701 cctccaggac cgcgccaccg gtcatccacg tctgacggga cgccgacgac gaccccgccg
     2761 acccgatgct cgtgtcgggc gtctcgacga cgacccgctg cacggaaagc gcggtgcgtg
     2821 cgatctgctc gcagaccgcg acgatgccct gccctacctc cgccgccgcc gtcagcacgt
     2881 acgcgaccgg ctccccgcgc tcccggacca gccggacgcg tgcggtggag aagtcgtcga
     2941 agccgtgcga gtagcagacg ttcttgaacc cggccgcgag ggcggtcccg cgccgcacgt
     3001 cttcgcctcg cgtg
//
LOCUS       DASTZR010000025         2040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1176647, whole genome shotgun sequence.
ACCESSION   DASTZR010000025 DASTZR010000000
VERSION     DASTZR010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176647"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..912)
                     /locus_tag="VFV20_00540"
     CDS             complement(79..912)
                     /locus_tag="VFV20_00540"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017302056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEU5286866.1"
                     /translation="MKRAAFFAYGVVCYAIFFGTFLYAIGFIGGFGVPTTLDGARRGS
                     LAQALAIDAGLLALFAVQHSVMARRWFKERWTRVVHPALERSTYVLLSSVALLLLFWQ
                     WRPLGGSVWSVDDPAGRAALYALYGFGFLLVLVATFLINHFDLFGLRQVWLFLRGVPY
                     TRLHFGTPGPYRFVRHPLYVGWFFAFWATPTMTYSHLLFAMATTAYILIAIQFEERDL
                     VHEHGEDYEAYRRAVPMLVPFTRLSMRRSARRAGIAALAFFFVKGLFWLGLGALALFA
                     S"
     gene            914..>2040
                     /locus_tag="VFV20_00545"
     CDS             914..>2040
                     /locus_tag="VFV20_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5286867.1"
                     /translation="MVLHDRPRARAVVWLCCPGIAHHTSIRAGQMSQPRQRIRFLDTS
                     DGVKLAWAEAGRGPLLVKAANWMTHLEYEWESPTWRHWLLFFSEHFRYVRYDERGCGL
                     SDWQVSDFSVERWAEDLAAVIDASRPDGPVALLGISQGAAACIAYAVRHPDRVARMVL
                     YGGYARGHNHRKNPTALTEFAVVKDAIRVGWGKENPAFRALFTSRFLPEGTPEQLASF
                     NELCRRTTTGEVAAALMEARASVNVEDLLPQVRVPTLVIHAQQDQVIPASEGRRLAAG
                     IPDAQFVQIESKNHVLLENEPAWPVFQDTVLEFFGVTASRGAHDPFATLSKREREILA
                     LVDEGLTNAQIAERLAISEKTVRNHASSLYDKLGVWSRAQAV"
BASE COUNT          383 a          704 c          669 g          284 t
ORIGIN      
        1 tcacccggtc ccgctccgcg ggaccaccct ctccccgcgc gctggacgcg cggggagagg
       61 gatcctgatg ttggtcgttc acgacgcgaa caacgccagc gcgccgagac ccagccagaa
      121 gagccccttc acgaagaaga acgcgagcgc tgcgatcccc gcgcgccgcg cgctccgccg
      181 catcgagagg cgcgtgaacg gcacgagcat cgggaccgcg cgccggtacg cttcgtagtc
      241 ctcgccgtgc tcgtgcacga ggtcgcgctc ctcgaactgg atcgcgatga ggatgtatgc
      301 cgtcgtcgcc atcgcgaaca gcagatgcga gtacgtcatc gtcggcgtcg cccagaacgc
      361 gaagaaccag ccgacgtaga gcgggtgacg cacgaaccgg tacggtccgg gcgtcccgaa
      421 atgcagcctc gtgtacggaa ccccgcgcag gaacagccac acctgccgca ggccgaagag
      481 gtcgaagtgg ttgatcagga acgtcgcgac caggacgagc aggaacccga acccgtagag
      541 agcgtagagc gcggcgcgac ccgccggatc gtcgacggac cagaccgagc cgccgagagg
      601 acgccactgc cagaagagca gaaggagagc gacgctggag agcaggacgt aggtcgagcg
      661 ctcgagcgcc gggtgaacga cgcgcgtcca gcgctccttg aaccagcgcc tcgccatcac
      721 gctgtgctgc acggcgaaga gcgcgagcag ccccgcgtcg atcgcgagcg cctgcgccaa
      781 cgacccccgc cgggcgccgt ccagcgtcgt cgggacaccg aacccgccga tgaagccgat
      841 ggcatagagg aacgtcccga aaaagattgc gtaacagacc accccgtaag cgaagaacgc
      901 ggctcgtttc atcatggtgc tccatgatcg gccccgggcg cgcgcagtgg tatggttatg
      961 ttgtcccgga atcgcgcacc acacctcgat ccgagcaggg caaatgtccc agccgcggca
     1021 acgaatccgt tttctggaca ccagcgacgg tgtcaagctc gcctgggcgg aggcgggccg
     1081 cgggccgctt ctggtcaaag cggccaactg gatgacccac ctcgagtacg agtgggagag
     1141 cccgacctgg cggcactggc tgctcttttt cagcgagcac tttcgctacg tccgctacga
     1201 cgagcgcggc tgcggtctga gcgactggca ggtaagcgac ttctcggtcg agcggtgggc
     1261 ggaagacctc gccgccgtca tcgacgcctc gcgccccgac ggtcccgtcg cgctcctcgg
     1321 catctcgcag ggcgccgcgg cctgcatcgc gtacgcggtc cggcaccccg accgggtcgc
     1381 gcggatggtc ctctacggag gctacgcgag aggccacaac cacaggaaga atcccaccgc
     1441 tctcaccgag ttcgccgtgg tcaaggacgc gatccgggtg ggatggggaa aagagaaccc
     1501 cgcgttccgc gcgctcttca cctcccgctt ccttccggaa ggaacgcccg agcagctcgc
     1561 ttccttcaat gagctctgcc ggcgcaccac cacgggcgaa gtcgcggcgg cgctcatgga
     1621 ggcgcgcgca tccgtcaatg tcgaggacct tcttccgcag gtccgcgttc cgaccctcgt
     1681 gatccatgcg caacaggatc aggtgatccc cgcatccgag gggcgccggc tcgcagccgg
     1741 gatccccgat gcccagttcg ttcagatcga gtcgaagaat cacgtcctgc tcgagaacga
     1801 gccggcgtgg cccgtgttcc aggacaccgt tctcgagttc ttcggcgtga ccgcaagccg
     1861 gggagcgcac gatccgttcg cgacgctctc gaagcgggag cgggagatcc tcgctctcgt
     1921 cgacgagggg ctgaccaacg cgcagatcgc cgagcgcctc gccatctccg agaagacggt
     1981 gcgcaaccac gcgtcgagcc tctacgacaa actgggcgtc tggagccgcg cgcaggccgt
//
LOCUS       DASTZR010000026         2166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_628769, whole genome shotgun sequence.
ACCESSION   DASTZR010000026 DASTZR010000000
VERSION     DASTZR010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628769"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1620)
                     /locus_tag="VFV20_00550"
     CDS             complement(7..1620)
                     /locus_tag="VFV20_00550"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019670430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEU5286868.1"
                     /translation="MPSTHDVVIRGGTLYDGSGRAPIPGDVAIDGDRIAAVGRVTARG
                     ATELDARGMAVAPGFINMLSWATDALFADGRSQSDIRQGVTLEIFGEGWSLGPLNERM
                     RRQRTAEQGDIKYDITWTTLLEALETLAARGISTNIASFVGATTVRMHEVGEDDRAPT
                     AQELARMRALVDRAMRDGALGVGSSLIYAPAAYATTDELVALCEAAAPYGGMYISHLR
                     SEGARLLEAIDELIDIARRAHVPAEIYHLKQAGQRNWPKLDGAIERVERARSQGLAIT
                     ADMYPYEAGATGLNACFPPWAHDGGPDALHARLRDATDRARIRDEMSRPATSWENLYD
                     AAGGPDGILVVSFKSERLKPFTGKTLAAIARERGGDPRDVAMDLVAEDESRVGMIVFM
                     GAKANLVREAALPWMSFGSDAGSLAPEGVFLRSQPHPRAYGTFARVLGRYVREEHAMP
                     LAEAVRRMTSFPAANLKLAHRGSLAPGFFGDVVVFDSETIADRATYERPHQYATGVRH
                     VLVNGTPVLRDGEHTDARPGRVVRGPGAR"
     gene            1650..>2166
                     /locus_tag="VFV20_00555"
     CDS             1650..>2166
                     /locus_tag="VFV20_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286869.1"
                     /translation="MYGLTRDETALLRSLRTPAKVQAYLDGLAYNLEPDGDTLRSPRR
                     VMRDRTAHCAEGAIFAAAAFRMNGRPPLIVDLEAVRDDDHVLAVYRDDGLWGAVATSK
                     FAGLRFRAPVYRTVRELVMSYFEDYYNWDGERTLRACSRPVSLARFDRIAWMTAEEDL
                     WPIVEYLAVAPH"
BASE COUNT          295 a          775 c          772 g          324 t
ORIGIN      
        1 cggccgtcat cgcgcgccag gaccgcgcac gacccggccg ggccgcgcgt cggtgtgctc
       61 gccgtcacgg agcaccggcg tgccgttcac gagcacatgc cggacaccgg tcgcgtactg
      121 atgtggccgc tcgtaggtcg cgcggtcggc gatcgtctcg gagtcgaaga ccacgacgtc
      181 cccgaagaac cccggcgcga gcgagccgcg gtgcgcgagc ttcaggttcg cggccgggaa
      241 ggacgtcatc cgtcgcacgg cctccgcgag cggcatcgcg tgctcctcgc gcacgtaacg
      301 gccgagcacg cgcgcgaatg tcccgtaggc gcgcgggtgc ggctgggacc ggaggaacac
      361 gccctcgggc gcgagtgacc cggcgtcgga gccgaagctc atccatggca gggcggcctc
      421 gcgcacgagg ttggccttcg cgcccatgaa cacgatcatg cccacgcggc tctcatcctc
      481 ggccacgagg tccatggcga catcgcgtgg atcgccgccg cgttcccgcg cgatcgccgc
      541 gagcgtcttg ccggtgaagg gcttcaggcg ctcgctcttg aacgacacga ccaggatgcc
      601 gtccggtccg ccggccgcgt cgtagaggtt ctcccaggac gtcgccggcc gggacatctc
      661 gtcgcggatg cgcgcgcggt cggtcgcgtc gcggagacgc gcgtggagcg cgtccggccc
      721 cccgtcgtgc gcccacggcg gaaagcacgc gttcagaccc gtggcgccgg cctcgtaggg
      781 gtacatgtcc gcggtgatcg cgagaccctg actccgggcg cgctccacgc gctcgatcgc
      841 gccgtccagc ttcggccagt tgcgctgccc ggcctgcttc aggtggtaga tctccgccgg
      901 aacatgtgcg cgccgcgcga tgtcgatgag ctcgtcgatg gcctcgagca ggcgtgcccc
      961 ctcgcttcgc aggtgagaga tgtacatgcc gccgtacggc gccgccgcct cgcagagcgc
     1021 gacgagctcg tcggtcgtgg cgtacgcggc cggcgcgtag atgagcgacg atcccacgcc
     1081 gagcgcgccg tcgcgcatcg cgcgatccac gagcgcccgc atgcgtgcga gctcttgcgc
     1141 cgtgggcgcg cgatcgtcct caccgacctc gtgcatgcgc acggtcgtcg cgccgacgaa
     1201 cgacgcgatg ttggtcgaga tgccgcgtgc cgcgagcgtc tcgagcgcct cgaggagggt
     1261 cgtccaggtg atgtcgtact tgatgtcgcc ctgctcggcg gtgcgctggc ggcgcatgcg
     1321 ctcgttcagc ggcccgagcg accagccctc gccgaagatc tcgagcgtga cgccctggcg
     1381 tatgtccgat tgcgagcgac cgtcggcgaa gagcgcgtcg gtcgcccagg acagcatgtt
     1441 gatgaacccg ggcgccacgg ccatgccccg tgcgtccaac tccgtcgccc cacgcgcggt
     1501 cacgcggccg acggcggcga tgcgatcgcc gtcgatcgcg acatcgccgg ggatcggcgc
     1561 gcggccgctg ccgtcgtaca gcgtcccgcc gcggatgacg acgtcgtgtg tggaaggcac
     1621 gcgcgccgat gttaggtcag actgcgcgca tgtacggcct cacgcgcgac gagaccgcgc
     1681 tcctgcgttc gctgcgcacg cccgcgaagg tacaggcgta cctcgacggc ctcgcgtaca
     1741 acctcgagcc cgacggcgac acgttgcgct cgccgcgccg tgtgatgcgg gaccgcaccg
     1801 cgcactgcgc cgagggcgcg atcttcgccg ccgcggcgtt ccgaatgaac ggacggccgc
     1861 cgctcatcgt ggacctcgag gccgtgcgcg acgacgacca cgtgctcgcc gtgtaccgcg
     1921 acgatggtct ttggggcgcg gtcgcgacgt cgaagttcgc cggactgcgg ttccgcgcgc
     1981 cggtctaccg cacggtgcgc gagctggtga tgagctattt cgaggactac tacaactggg
     2041 atggcgagcg gacgctccgt gcatgctcgc gtcccgtctc gctcgcgcgc ttcgaccgga
     2101 tcgcgtggat gaccgcggag gaggacctct ggcccatcgt ggagtacctc gcggtggcgc
     2161 cgcaca
//
LOCUS       DASTZR010000027         7740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_80176, whole genome shotgun sequence.
ACCESSION   DASTZR010000027 DASTZR010000000
VERSION     DASTZR010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7740
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80176"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VFV20_00560"
     CDS             <1..514
                     /locus_tag="VFV20_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286870.1"
                     /translation="IQYVGSHANRTAALLAGTIDAGVDDPPEDQELVKAGLNPLINLA
                     AQKLPASNTGIIVQRSYLSANKDTIQAYIDSTIQARAKMGKDKDGAITVLKKYFKSDD
                     TESMTHAYNFFIAGTSPVTPLYPAPGPDQLKDAVTILGADNDKIKTVDINKMLDASFV
                     KSAQDRKLGG"
     gene            588..1691
                     /locus_tag="VFV20_00565"
     CDS             588..1691
                     /locus_tag="VFV20_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008172155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5286871.1"
                     /translation="MSETETTAEQAKFLSDPYREWAGREGIPIHEDFGFDLLSLELAP
                     WGRMEARGAFAHCLGRGDFANMYVCEIAPGGRTAPQRHLYEEVVYVLSGRGSTTIEAP
                     EGERSFEWHEGSLFALPLNARYRHFNGSGQRPARFAAVTDLPLVLNLFRNEAFIWDND
                     TAFPERFGEPRYNQGEGTFIPKQPGRHMWETNFVPDLRTLELQTWDKRGGGSRNIMFV
                     LADGTMHAHVSEMPVGTYKKGHRHGADFHVFTVKGTGYSLYWYEGDTDFKRFDWKHGA
                     VFTPTDMLFHQHFNTSAEPARYLAIAFGGLRYPTLGDKRKTFAGMDVSVKDGGRQIEY
                     EDEDPRIRRIYEEELRKNGVASKMDEVFAAATR"
     gene            complement(1750..2790)
                     /locus_tag="VFV20_00570"
     CDS             complement(1750..2790)
                     /locus_tag="VFV20_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020509627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5286872.1"
                     /translation="MGEDQRTFLRGFGSETYGLKDFRAKQRSLDRVRKKGTVVDDASV
                     GHSGDSDEGKSKTYWMVGPGDKEFLSQTLQVHFVELIPGGENHGHGHQNEAHFYILEG
                     AGYEIHDGQRYQWKKDDLVIVHTDSKHKHFNLSTTERGLALVLKAKTTWMLLGMWQQG
                     RSAEFADDSKYLPRMDWGANLWTPGWEKQKKVITPADTKWETTRDGKVRVILSKDRTD
                     ARTHSLDLYQQEIAPGSRSAKHWHMADEMVYVLSGSGQSLQWDVEAEIDDKYYARVPK
                     EPSTWSFGAGDVVYVPTNTVHQLVNTGREPLMVLVAQNRVFKFIGYDNVYFFEDAPEY
                     AGKGAKQAVAAD"
     gene            complement(2783..4021)
                     /locus_tag="VFV20_00575"
     CDS             complement(2783..4021)
                     /locus_tag="VFV20_00575"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5286873.1"
                     /translation="MRSPRGSYLLMLAVATSITTTKGTVEIIYPPYLAGYGYSLSIIG
                     FLTSLIAVLQLASRVPAGVAYRSHRAKQQYALALVAFGLSTLGFAFAGGQGLAVAGLS
                     ALQGFAFGTLGTLGLALAIDISGDRAVGASMAWYTAAISTGYALGSLIGGTLAETIGM
                     PATLGLIGFLPFVAALAVMRLPAVEGAPQAFQRGSGLRGLVASGARLDSRVWLAVVIV
                     LYLNLVQDSQDTFFPVYAPAVGMSLAVVGLLRAIKSGAGIFIRFGIAVLLHSLDYRRI
                     TLVTVVASAIAAMAIPLSSSLFALVPIFVVMGLARGVLRATSAATIAELRREGKDVGL
                     ASGVYNAGLDIGSIIGPTVGGAIATAFGIGPMFQIVAGASLVAFLCVALATPATREAA
                     GLAKRHTIGPTPISEGEHSG"
     gene            complement(4039..4635)
                     /locus_tag="VFV20_00580"
     CDS             complement(4039..4635)
                     /locus_tag="VFV20_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEU5286874.1"
                     /translation="MSTPRRIVVAISGASGAIYGIRAVEAIHADRGIELHLVVSSGAK
                     ATIGYETDRTVEEIAKLADVVHEEKDLAATIASGSFQTHGMLIAPCSIRTMASVANAM
                     NDNLIVRAADVHLKERRTLVLMVRETPLNATHLRLMHELSLAGAVILPPVPAFYHRPK
                     TLMDLVDHSVGKALDQLGVAHTLFERWGGIDKTSSTAR"
     gene            complement(4646..5410)
                     /locus_tag="VFV20_00585"
     CDS             complement(4646..5410)
                     /locus_tag="VFV20_00585"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEU5286875.1"
                     /translation="MFTPVRQSRASGEIVSQIERAVFAGELTAGDRLQSERELAEQFG
                     VSRITVRDALRVLEARGLIRVKVGAMGGAFVADANTDRVAESISTMIKLKRMTLSELA
                     EARTIVEAATASLAAQRADAAAVSRLQQSVEGARTLLREPITHADASMDFHVVLARAA
                     RNEVLEATVEAYRQLLVPSIRDLRDDKSGRATQKAHEELVEAVRRHDPEGARDIMLTH
                     LADFEKRLRRSLATRADGAAAPALVAPRLVRVRRVK"
     gene            5432..6709
                     /locus_tag="VFV20_00590"
     CDS             5432..6709
                     /locus_tag="VFV20_00590"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5286876.1"
                     /translation="MSTLFPGLHPLRHRNFALYFCGLTVSQVGDWIETTTTAWLLYEI
                     THDPVLLGVGGGIRALGIILFGLIGGAVADRTDRRRLLFIMQTGFALSSLALGLLVLT
                     GTVTFWHIYVFSAVNGSLGAFEAPARRSLFPNLVPRADMQNAVVLNSAVFRLARLTGP
                     AIAGVLIATQGPAISYFVNFVSYAAILLALVAMQVPPMPVRRTASLLREVADGLRYTL
                     RRPLLRAVLVLESTHSLFGVNTALITIIASDVLHAGPQGLGLLLSAQAFGALVGTTAL
                     MMTGDMEHKGRAMMAAGATYCVALALLAVAQRFEVAAILLVLTAVTDAFWTAMRNTMF
                     QLQTDEAYRGRSLSTVLLAGRGFTQMAQLETGVAVSVGGPAFAVVSGAAVIAAVLLGV
                     NARTDEVRSFSGSPDAVAAVAAASQDGGDGGAS"
     gene            complement(6730..7428)
                     /locus_tag="VFV20_00595"
     CDS             complement(6730..7428)
                     /locus_tag="VFV20_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEU5286877.1"
                     /translation="MSERDRIAECAVATRVLAVAGLFDMHGHISVRDGDVIYICDRGA
                     SRLTVTPELIASVRVSDGARLSEALPPSETALHLAAYRARPEVRSVAHFHPLYATAFA
                     VAGVPLVPASNAGAFFGGAIPVHDNPELIRSDALGVPVARDMGAATALLLRGHGALVV
                     GGDIPECATAALYLEESAQRNWLAASLGSVKAYTADDIRRVREMLASRFVIDKTWLYA
                     LEKARVAGALAGLE"
     gene            complement(7425..>7740)
                     /locus_tag="VFV20_00600"
     CDS             complement(7425..>7740)
                     /locus_tag="VFV20_00600"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEU5286878.1"
                     /translation="HDSQQDLAAAAGKSADGPDKFAALRIRTVEAPAGLRAPGVEGAV
                     AVLWCRVANTVETGDHLLFVGRVEEFRVDDRRWDPLLRYGRRYHRLGHWTSDESPEGY
                     PT"
BASE COUNT         1173 a         2710 c         2701 g         1156 t
ORIGIN      
        1 gatccagtac gtcgggtcgc acgcgaaccg caccgcggcg ctcctcgcgg gcacgatcga
       61 cgccggcgtc gacgacccgc ccgaggacca ggagctcgtg aaggcgggac tcaacccgct
      121 catcaacctc gcggcgcaga agctgcccgc gtccaacacc ggcatcatcg tgcagcgcag
      181 ctacctgagc gcgaacaagg acaccatcca ggcgtacatc gactcgacga tccaggcccg
      241 ggccaagatg ggcaaggaca aggacggcgc gatcaccgtc ctgaagaagt acttcaagag
      301 tgacgacacc gagtcgatga cgcacgcata caacttcttc atcgcgggca cgagcccggt
      361 gacgccgctc tatccggccc cgggtcccga ccagctgaag gacgccgtca cgatcctcgg
      421 cgccgacaac gacaagatca agaccgtcga catcaacaag atgctcgacg cgtccttcgt
      481 gaagagcgcg caggaccgca agctcggcgg ctaacgcgac tcatgcggac ttcgacgtgg
      541 aggagggcga cgccctcctc cacgcgtgta tggaggatgg agggccgatg agcgagaccg
      601 agacgacggc ggagcaggcg aagttcctca gcgacccgta ccgcgaatgg gcggggcgcg
      661 agggcatccc catccacgag gacttcggct tcgacctgct ctccctcgag ctcgcgccgt
      721 ggggtcgcat ggaggcgcgc ggcgcgttcg cgcactgcct cggccgcggc gacttcgcga
      781 acatgtacgt ctgcgagatc gcgcccggtg ggcgcaccgc gccgcagcgc catctgtacg
      841 aggaggtcgt ctacgtgctg tcgggccgcg gcagcaccac catcgaggcg cccgagggcg
      901 agcgtagctt cgagtggcac gagggcagcc tgttcgcgct cccgctcaac gcgcggtacc
      961 ggcacttcaa cggcagcggc cagaggccag cgcgcttcgc cgccgtcacc gacctgcccc
     1021 tcgtgctcaa cctcttccgt aatgaggcgt tcatctggga caacgacacg gccttcccgg
     1081 agcgattcgg cgagccgcgc tacaaccagg gcgagggcac gttcatcccg aagcagccgg
     1141 gccggcacat gtgggagacg aacttcgtgc ccgacctgcg cacgctcgag ctgcagacgt
     1201 gggacaagcg cggcggcggc tcgcgcaaca tcatgttcgt cctcgccgac ggcacgatgc
     1261 acgcgcacgt ctccgagatg cccgttggca cgtacaagaa gggccatcgc cacggcgcgg
     1321 acttccacgt cttcaccgtg aagggcacgg gctacagcct gtactggtac gagggcgaca
     1381 ctgacttcaa acgcttcgac tggaagcacg gcgcggtgtt cacgccgacc gacatgctct
     1441 tccatcagca cttcaacacc agcgcggagc cggcgcgcta cctcgcgatc gcgttcggcg
     1501 gccttcgcta cccgaccctc ggcgacaagc gcaagacgtt cgccggtatg gacgtgagcg
     1561 tcaaggacgg cgggcgccag atcgaatacg aggacgagga tccgcgcatc cgccgtatct
     1621 acgaggagga gctccggaag aacggcgtcg cgtcgaagat ggacgaggtg ttcgcggccg
     1681 cgacgcgcta gcgaaaggac gcacgcggcc gccgcggcga aacgccacga cggccgcgcc
     1741 gatcgtcgtt cagtcggcgg ccacggcctg cttcgcgccc ttgccggcgt actccggagc
     1801 gtcctcgaag aagtacacgt tgtcgtagcc gatgaacttg aacacgcggt tctgcgcgac
     1861 cagcaccatc aggggctcgc gcccggtgtt cacgagctgg tgcacggtgt tcgtcggcac
     1921 gtagacgacg tcgccggcgc cgaagctcca cgtgctcggc tccttgggca cgcgcgcgta
     1981 gtacttgtcg tcgatctcgg cctcgacgtc ccactgcagg ctctggccgc tgccggagag
     2041 gacgtagacc atctcgtcgg ccatgtgcca gtgcttcgcc gagcggctgc cgggtgcgat
     2101 ctcctgctgg tagaggtcga ggctgtgcgt gcgcgcgtcg gtgcgatcct tcgagaggat
     2161 cacgcgcacc ttcccgtcgc gggttgtctc ccacttcgtg tccgcgggcg tgatgacctt
     2221 cttctgcttc tcccagccgg gcgtccacag gttcgcgccc cagtccatgc gcggcaggta
     2281 cttgctgtcg tccgcgaact ccgcggaacg gccctgctgc cacatgccga ggagcatcca
     2341 tgtggtcttg gccttcagca cgagggcgag gccacgctcg gtggtcgaga ggttgaagtg
     2401 cttgtgcttg ctgtcggtgt ggacgatcac gaggtcgtcc ttcttccact ggtagcgctg
     2461 gccgtcgtgg atctcgtagc cggcaccctc gaggatgtag aagtgcgcct cgttctggtg
     2521 tccgtgcccg tggttctcgc cacccgggat gagctccacg aagtggacct ggagggtctg
     2581 gctcaggaac tccttgtcac ccgggccgac catccagtag gtcttggact tgccctcgtc
     2641 ggagtcaccg gagtgaccga ccgaggcgtc gtcgacgacg gtgcccttct tacgcacccg
     2701 gtcgagactg cgctgcttcg cgcggaaatc cttcagaccg tacgtctctg atccgaagcc
     2761 gcgcaggaat gtgcgctggt cctcacccac tgtgctctcc ttcgctgatc ggtgttggtc
     2821 caatggtatg acgtttcgcg agcccggccg cttctcgcgt cgcgggcgtg gcgagagcga
     2881 cacacaggaa ggcgaccagg ctcgcgccgg cgacgatctg gaacatcggc ccgatgccga
     2941 acgcggtcgc gatcgcgcca ccgaccgtcg ggccgatgat gctgccgatg tccaggcccg
     3001 cgttgtagac gccggatgcg agcccgacgt ccttgccctc gcggcggagc tccgcgatgg
     3061 tcgccgcgct cgtcgcgcgc aggacgccgc gcgcgaggcc catcacgacg aagatcggga
     3121 cgagcgcgaa gagcgagctc gagagcggga tggccatcgc ggcgatcgcc gacgcgacca
     3181 cggtgacgag cgtgatgcgc cggtagtcga gcgagtggag cagcactgcg atgccgaagc
     3241 ggatgaagat gcccgcgccg ctcttgatcg cgcggagcag gccgacgacg gcgagagaca
     3301 tcccgacggc cggcgcgtac accgggaaga acgtgtcctg gctgtcctgc accaggttga
     3361 gatacagcac gatcacgacg gcgagccaca cgcgactgtc gagccgcgca ccggaggcga
     3421 ccaggccgcg gagcccgctg ccgcgctgga acgcctgcgg cgcgccctcg accgcgggga
     3481 ggcgcatcac cgcgagcgcg gcgacgaacg gcaggaaccc gatgagcccc agcgtcgcgg
     3541 gcatgccgat ggtctcggcg agcgtgccgc cgatgagcga tccgagcgcg tacccggtcg
     3601 agatcgcggc ggtgtaccag gccatcgacg cgccgaccgc gcggtcgccg ctgatgtcga
     3661 tggcgagcgc gaggccgagc gtcccgaggg tcccgaaggc gaagccctgc agcgccgata
     3721 gtccggccac ggcgaggccc tgcccgcccg cgaaggcgaa gccgagcgtc gacaggccga
     3781 acgcgacgag cgcgagggcg tactgctgct tcgcgcggtg cgagcggtac gcgacgccgg
     3841 cgggcacacg ggacgcgagc tgcagcacgg cgatgagcga cgtgaggaag ccgatgatcg
     3901 agagcgagta gccgtacccg gccaggtacg gcggatagat gatctcgacg gtccccttcg
     3961 tcgtggtgat cgacgtcgcc acggcgagca tgagcaggta cgacccacgc gggctgcgca
     4021 taagcggggc cgcgaccgtc accgcgcggt cgaggaggtc ttgtcgatgc cgccccagcg
     4081 ctcgaagagc gtgtgcgcga cgccgagctg gtcgagggcc ttgccgacgc tgtgatcgac
     4141 gaggtccatg agcgtcttcg ggcggtgata gaacgccggc accggcggca ggatcaccgc
     4201 gccggcgagg ctgagctcgt gcatgagccg gaggtgcgtc gcgttgagcg gcgtctcgcg
     4261 gaccatcagc acgagggtgc gccgctcctt caggtggacg tcggcggcgc gcacgatgag
     4321 gttgtcgttc atggcgttcg cgaccgatgc catcgtgcgg atgctgcagg gcgcgatcag
     4381 catgccgtgc gtctggaagg agccactcgc gatcgtcgcg gcgaggtcct tctcctcgtg
     4441 gacgacgtcc gcgagcttcg cgatctcctc gacggtgcgg tcggtctcgt agccgatcgt
     4501 cgccttcgcg cccgaggaga cgaccagatg cagctcgatc ccgcggtccg cgtggatcgc
     4561 ctcgaccgcg cggatgccgt agatcgcgcc ggacgcgccg gagatcgcga cgacgatgcg
     4621 gcgcggcgtg ctcaacgggt cgtggctact tcacccgccg gacgcgcacg aggcgcggcg
     4681 cgacgagcgc aggtgcggcc gcgccgtcgg cgcgcgtcgc gaggctgcgg cgcagccgct
     4741 tctcgaagtc ggcgaggtgc gtgagcatga tgtcgcgcgc gccctcgggg tcgtggcgac
     4801 gtacggcctc caccagctcc tcgtgggcct tctgcgtcgc gcggccggac ttgtcgtccc
     4861 gcaggtcgcg gatggacggg acgaggagct gccggtacgc ctcgacggtc gcctcgagca
     4921 cctcgttgcg cgccgcgcgc gccagcacga cgtggaagtc catgctcgcg tccgcgtgcg
     4981 tgatcggctc gcgcaggagc gtccgcgcac cctcgacgct ctgctgcagg cggctcaccg
     5041 ccgcggcgtc ggcgcgctgg gccgcgagcg aggcggtggc ggcctcgacg atcgtgcggg
     5101 cctccgccag ctccgagagc gtcatccgct tgagcttgat catcgtcgag atcgactcgg
     5161 cgacacggtc ggtgttcgcg tcggcgacga acgcgccgcc catcgcgccg accttcaccc
     5221 ggatgaggcc gcgcgcctcg agcacccgca gtgcgtcacg gaccgtgatg cgcgagacgc
     5281 cgaactgctc cgcgagctcg cgctcggact ggagccggtc gccggccgtc agctcgcccg
     5341 cgaacacggc gcgctcgatc tgcgagacga tctcgccgct cgcgcgcgat tgccgcacgg
     5401 gtgtgaacac cgcgagaatg ctactgctgc gttgagcact ctctttccgg gactccatcc
     5461 gctccgccac cgcaacttcg cgctgtattt ctgcggcctc acggtctccc aggtgggcga
     5521 ctggatcgag accacgacca ccgcctggct gctgtacgag atcacgcacg atcccgtcct
     5581 cctcggcgtg ggcggcggca tcagggcgct cgggatcatc ctcttcggcc tcatcggcgg
     5641 ggccgtcgcg gaccgcaccg accgccgccg cctgctcttc atcatgcaga cggggttcgc
     5701 gctctcgtcg ctcgcgctcg gcctgctggt gctcacggga acggtgacgt tctggcacat
     5761 ctacgtgttc agcgcggtga acggctcgct cggcgcgttc gaggcgccgg cgcggcggtc
     5821 gctcttcccg aacctcgtcc cgcgcgcgga catgcagaac gcggtcgtcc tgaactccgc
     5881 ggtgttccgc ctcgcccggc tcaccggccc ggcgatcgcc ggcgtgctca tcgcgacgca
     5941 gggaccggcg atctcgtact tcgtcaactt cgtgagctac gccgcgatcc tgctcgcgct
     6001 ggtggccatg caggtgccgc cgatgccggt ccggaggacc gccagcctgc tgcgcgaggt
     6061 cgccgacggc ctgcggtaca cgctgcggcg cccgctgctg cgggccgtgc tcgtgctcga
     6121 gtcgacgcac agcctcttcg gcgtgaacac cgcgctcatc acgatcatcg cctccgacgt
     6181 gctccatgcc ggaccccagg gcctgggcct gctgctctcg gcgcaggcct tcggagcgct
     6241 cgtcggcacg accgcgctga tgatgaccgg cgacatggag cacaagggcc gcgcgatgat
     6301 ggcggccggc gccacgtact gcgtggcgct cgcgctgctc gcggtggcac agcgcttcga
     6361 ggtcgccgcg atcctgctcg tcctcaccgc ggtgaccgac gcgttctgga ccgcgatgcg
     6421 aaacaccatg ttccagctgc agaccgacga ggcctaccgc gggcgcagcc tgtccaccgt
     6481 cctgctcgcg ggccgcggct tcacgcagat ggcccagctc gagaccggag tcgcggtctc
     6541 cgtcggcggg cccgcgttcg ccgtcgtgtc gggcgccgcg gtcatcgcgg ccgtgctgct
     6601 cggcgtgaac gcgcgcaccg acgaggtacg gtcgttcagc ggatcgcctg atgccgtcgc
     6661 ggccgtcgcc gcggcgagcc aggacggcgg cgacggaggc gcgtcctagc gcccgttcgt
     6721 gcgctcgcgc tactcgaggc cggcgagcgc cccggcgacg cgcgccttct cgagcgcgta
     6781 cagccaggtc ttgtcgatga cgaagcggct cgcgagcatc tcgcggacgc ggcggatatc
     6841 gtccgcggtg tacgccttca cgctcccgag gctcgccgcg agccagttcc gctgcgcgct
     6901 ctcctcgagg tacagcgcgg cggtcgcgca ctcagggatg tcgccgccga cgacgagcgc
     6961 accatggccg cgcagcagca gcgcggtcgc cgcgcccatg tcgcgcgcga caggcacgcc
     7021 gagcgcgtcg ctgcggatga gctcggggtt gtcgtgcacc gggatggcgc cgccgaagaa
     7081 cgcccccgcg ttcgacgccg gcacgagcgg gacgcccgcg accgcgaatg ccgtcgcgta
     7141 cagcggatgg aagtgcgcga cgctccgcac ctcgggccgc gcccggtacg ccgcgaggtg
     7201 cagcgcggtc tccgatggcg gcagcgcctc cgagagccgc gcgccgtcgg acacgcgcac
     7261 cgacgcgatg agctcgggcg tgacggtgag ccggctcgcg ccgcgatcac agatgtagat
     7321 cacgtcgccg tcgcgcaccg agatgtggcc gtgcatgtcg aagagccccg cgaccgcgag
     7381 cacgcgcgtc gcgacggcgc actcggcgat gcggtcgcgc tcgctcacgt cgggtagccc
     7441 tcgggcgact cgtcgctcgt ccagtggccc aggcggtgat agcggcgacc atagcgaagg
     7501 agcggatccc agcgccggtc gtcgacgcgg aactcctcca cccgtccgac gaagagcagg
     7561 tggtcgccgg tctcgacggt gttcgccacg cggcaccaca ggacggcgac cgcgccctcc
     7621 acgccgggtg cgcgcagtcc cgccggcgcc tcgaccgtgc ggatgcgcag cgccgcgaac
     7681 ttgtcggggc cgtccgcgct cttccccgcc gcggcggcga ggtcctgctg ggagtcgtgc
//
LOCUS       DASTZR010000028         3214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1151179, whole genome shotgun sequence.
ACCESSION   DASTZR010000028 DASTZR010000000
VERSION     DASTZR010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151179"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VFV20_00605"
     CDS             complement(<1..230)
                     /locus_tag="VFV20_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEU5286879.1"
                     /translation="MRSDERAFVRPRRFIAMGLAIVLVFTSLTLRLWDLQVVNGAHYR
                     SLAEQNRVLRIPVMAERGNIVDRNGYVLARNIP"
     gene            complement(227..730)
                     /gene="mreD"
                     /locus_tag="VFV20_00610"
     CDS             complement(227..730)
                     /gene="mreD"
                     /locus_tag="VFV20_00610"
                     /inference="COORDINATES: protein motif:HMM:NF016019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEU5286880.1"
                     /translation="MRVAIGIALPLAAALLQGSVVPFIDVAGARPNLVVLMAASWSVA
                     AGAREAVWWAFFGGLAADLLSGGPLGALALASLVPVAAVGLAETQLRPRSVVAAAILV
                     GLASLATSVIYVGILALVGTALPSAPVLAAAVASGAVYNGVLAIATYPIARSLRRTTE
                     KQASFGW"
     gene            complement(787..1938)
                     /locus_tag="VFV20_00615"
     CDS             complement(787..1938)
                     /locus_tag="VFV20_00615"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU5286881.1"
                     /translation="MRSRWSPTAKRVAVATIVAVLAFFVWRAGDIVQPFIWATILAYV
                     LLPLVGSLERRFSFPRTAAALVVFVALLAVIFGGVRLLLPRLADNTHDLQQNWPVLFA
                     NARQTVSDTFTQIGLGDLDDALIGPNLQDLERQLGAMASRNALPFVLGAGHFLLEFLI
                     FLIATFLMLRDAPRLLDAVRRNLPRQHRSEILHVLRDTNVMLGRYIRGQLILVALMSS
                     VTTIALTLLGVPYSVLLGLMTGLLETIPFVGPITAGAIACVVALGHPNPFGWSQLAYV
                     AVVAIMYTVLRHAEDYLVIPTVIGRAVRLHPAVVIFALLTGGATFGLLGVVLAVPFAA
                     TMRLVLIYVRAKLRDEDPYAPLTEELAEATASGTEQIEPRVRPGVRAQS"
     gene            2184..2969
                     /locus_tag="VFV20_00620"
     CDS             2184..2969
                     /locus_tag="VFV20_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5286882.1"
                     /translation="MRVLVVSNQKGGVGKTTTAISLGAALVERGQRVLIVDLDPQANA
                     TSGLGIPKDLPGTYSVLLKEQPITESIVQTEVPGLDLLPSGPEMAGAEVELVPLMARE
                     FRLRQSLRLASAYDTVIVDCPPSLGLLTVNALAAGDGVLVPVQCEYYALEGLAQLLDT
                     IEAVRQRLNERLEVLAIVLTMEDRRNRLSMQVTDEVRKHFPKLVARTRIPRTVRLAEA
                     PSYGKPISVYDPSSRAAEAYNELAREIEQRLQSREPIALAGSV"
     gene            2969..>3214
                     /locus_tag="VFV20_00625"
     CDS             2969..>3214
                     /locus_tag="VFV20_00625"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEU5286883.1"
                     /translation="MARSGLGRGLDVLLGQPSGTAPGTAAVAVGATTEIPLDKVRPNP
                     QQPRLQFDAAGIEELAASIRRHGVLQPIVVSRNGDHYE"
BASE COUNT          580 a         1067 c         1130 g          437 t
ORIGIN      
        1 gggatgttcc gcgcgaggac gtatccgttg cggtcgacga tgttcccccg ctcggccatc
       61 acggggatgc gcaggacgcg gttctgctcc gcgagggatc gatagtgcgc gccgttgacc
      121 acctgcagat cccagagacg cagcgtcagg ctcgtgaaca ccaagacgat cgcgagcccc
      181 atcgcgatga agcggcgcgg cctcacgaac gcgcgctcgt ccgatctcac catccgaagg
      241 atgcctgctt ctcggtcgtc cggcgaaggc tgcgcgcgat cggataggta gcgatcgcga
      301 gcacgccgtt gtacacggcg cccgacgcca ccgccgcggc gagcaccgga gcgctcggga
      361 gcgccgtgcc gaccagggcg aggatgccga cgtagatcac gctcgtggcc agactcgcca
      421 gtcccacgag gatcgcggcg gcgaccacgg accggggccg gagctgcgtc tcggcgaggc
      481 cgacggcggc cacggggacg agcgacgcga gcgcgagcgc tccgagcgga ccgccggaga
      541 ggaggtccgc ggcgagcccg ccgaagaagg cccaccagac ggcctcgcgc gcgccggcgg
      601 cgaccgacca cgaggcggcc atgaggacga ccaaatttgg gcgcgcaccc gccacgtcga
      661 tgaacggcac gaccgacccc tgcaggagtg ccgcggcgag cgggagcgcg atgccgatgg
      721 ccacccgcac ggacttccct cctctggacc ggaacgcgcc acgcgctccg gcgctacgcg
      781 ggctcgctag gactgggcgc ggacgccggg acgcacgcgg ggctcgatct gctcggtgcc
      841 ggaggcggtc gcctccgcga gctcttccgt cagtggcgcg tagggatcct cgtcccggag
      901 cttcgcacgg acgtagatga ggacgaggcg catggtcgcg gcgaacggca ccgcgaggac
      961 gacgccgagg aggccgaacg tcgcgccgcc cgtgagcagg gcgaagatca cgaccgccgg
     1021 atgcaggcgg accgcccggc cgatgaccgt cgggatgacc aggtagtcct cggcgtggcg
     1081 gaggaccgtg tacatgatcg cgacgacggc cacgtacgcg agctgcgacc agccgaacgg
     1141 attcggatgg ccgagcgcga cgacgcacgc gatcgcgccg gcggtgatcg ggccgacgaa
     1201 cgggatcgtc tcgagcagcc cggtcatgag gccgagcagc acgctgtacg gcacaccgag
     1261 cagcgtgagc gcgatcgtcg tcaccgacga catgagggcg accaggatga gctgcccgcg
     1321 gatatagcgg ccgagcatga cgttcgtgtc acgaaggacg tggaggatct cggagcgatg
     1381 ctggcgcggg agattccggc ggacggcgtc gaggaggcgc ggcgcgtcgc gcagcatgag
     1441 gaacgtcgcg atcaagaaga tgaggaattc gagcaggaag tggccggccc cgagcacgaa
     1501 gggaagcgcg ttgcgcgagg ccatcgcgcc gagctggcgc tcgagatcct ggaggttcgg
     1561 cccgatgagc gcgtcgtcga gatcgccgag gccgatctgc gtgaaggtgt cggagaccgt
     1621 ctggcgcgcg ttcgcgaaca gcacgggcca gttctgctgg aggtcgtgcg tgttgtccgc
     1681 gagacgcggg aggagcaagc gaacgccacc aaaaatgaca gctagcagcg ccacgaagac
     1741 gacgagggct gccgcggtcc gcgggaagga gaaccggcgc tcgagtgatc ccacaagtgg
     1801 tagcaggacg tacgcgagga tcgtcgccca gatgaagggc tggacgatat cgcccgcgcg
     1861 ccagacgaag aacgccagga cggccacgat cgtcgccacg gcgacccgtt tcgcggtcgg
     1921 actccagcgc gatctcatcc cgctggcgat tatccggcag ggatccgaag ctgcgtcggg
     1981 actgcgcgac gtatggcctc gaatcgcaac acgcggcacg ctcgtagaac cgtttaagtg
     2041 cggtactgca gtctatgcag tatcacctcc tgaggcgccc ggacgcgctt tcgggtagaa
     2101 ttggaggcac gaacacgccc gtttccgttc ctagttttcc ccactttcct aggcgcatgt
     2161 tccacgtgtg acagaggcgt ggaatgaggg ttctcgtcgt gagtaaccag aagggcggag
     2221 tggggaagac gacgacggca atcagcctcg gcgcggcgct cgtggagcgc gggcagcgcg
     2281 tcctcatcgt cgacctcgac ccccaggcca acgcgacgag cggccttggc atccccaaag
     2341 acctgcctgg aacgtacagc gtcctcctca aggagcagcc gatcaccgaa tcgatcgtcc
     2401 agactgaagt tcccggcctt gacctcctgc cgtccggccc tgagatggcc ggcgcggagg
     2461 tggagctcgt gccgctgatg gcgcgtgagt tcaggctccg gcaatcactg cggctggcga
     2521 gcgcgtacga caccgtcatc gtcgactgcc caccatccct tggcctcctt accgtgaacg
     2581 ccctcgcggc gggggacggc gtgctcgtcc cggtccaatg cgagtactac gcgctcgagg
     2641 gcctcgccca gttgctcgac acgatcgaag cggtgcggca gcgcctcaac gagcggctgg
     2701 aggtcctcgc gatcgtgctc acgatggagg accgccggaa ccgtctctcc atgcaagtga
     2761 ccgatgaggt gcgaaagcac ttcccgaagc tcgtcgcccg cacgcggatc ccgcggaccg
     2821 ttcgccttgc agaggccccc agctacggca agccaatctc cgtctacgac ccgagctcac
     2881 gcgctgccga ggcctacaac gagctcgcgc gtgagatcga gcagcgtctc cagtcgcgcg
     2941 agccgatcgc acttgcgggg tcggtctgat ggcccgcagc ggtctcggcc gcggtctcga
     3001 cgtcctcctc ggtcagccga gcggaaccgc gcccggaacc gcggccgtag cggtgggcgc
     3061 gacgaccgag atcccactcg acaaggtccg gccgaacccc cagcaacctc gtctccagtt
     3121 cgacgcggcg ggcatcgagg agctcgcggc gtcgatccgc cgccacggcg tcctgcaacc
     3181 gattgtggta tctagaaatg gcgaccacta cgag
//
LOCUS       DASTZR010000029         2405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_132810, whole genome shotgun sequence.
ACCESSION   DASTZR010000029 DASTZR010000000
VERSION     DASTZR010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132810"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VFV20_00630"
     CDS             complement(<1..666)
                     /locus_tag="VFV20_00630"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031411 - gas vesicle [Evidence IEA];
                     GO_process: GO:0031412 - gas vesicle organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HEU5286884.1"
                     /translation="MSGLRYVYGIVPASVAPLVDGAALTGIDDAPVRTIAEGPFAATT
                     SELSDTEYAAETLNERIRDLDWLTPRAAAHQAVNERVLDIAGTVLPLSFGTLYRDDVR
                     VREMLREDVEQRARRLVALNGRAEWIVTLTRESETTLGTDEVTELDREIASSTPGRAF
                     LLEKRRATVAKAATERADAEAARRAEEALETVAERTYREPVARGGADTVVLRISLLAE
                     RSRT"
     gene            complement(663..986)
                     /locus_tag="VFV20_00635"
     CDS             complement(663..986)
                     /locus_tag="VFV20_00635"
                     /inference="COORDINATES: protein motif:HMM:NF016976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpG"
                     /protein_id="HEU5286885.1"
                     /translation="MLLRLLTLPISAPVAGIRYCLEKVAEVADKEMWDEGPVREQLIL
                     DNQAYEEGRLTEEEFRVREAELLLRLREIREHHKARAREEAGDAGAAVERQRHVVIDL
                     PEEMR"
     gene            complement(988..1758)
                     /locus_tag="VFV20_00640"
     CDS             complement(988..1758)
                     /locus_tag="VFV20_00640"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HEU5286886.1"
                     /translation="MGEGIYVYCIIESAEPRAFGKIGIGGRGDEVFTVHHRELAAVVS
                     RTPLMVYDPTRENALTHEHVNEVVMIDNAFTPVPMSFGTLFKTEDDIVEFLKDTYEAL
                     RDVLRKMKDKLEFGLKVNWDRDGVLADVERDYEEIRRLKAQIEGEQAQSTYFARMQLG
                     RLVEQALAEKSETFVREIYDELRESAIASRSNKVIGDRMIMNAAFLVERDKADMFDKK
                     VQEIGRRYEGKLKFTYTGPWPPYNFVTIRLQLERSAGV"
     gene            1896..1967
                     /locus_tag="VFV20_00645"
     tRNA            1896..1967
                     /locus_tag="VFV20_00645"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1928..1930,aa:Lys,seq:ttt)
     gene            complement(2098..>2405)
                     /locus_tag="VFV20_00650"
     CDS             complement(2098..>2405)
                     /locus_tag="VFV20_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286887.1"
                     /translation="TEVQFVSGIGHLAEAAIGLAVLVPNLRRHSRVEWLWAHTIALAY
                     WAFQIAVLTPPWFSFQGQREVVTGAALAFVSLGAAVNAALWVRAASAAPGRRVPRLD"
BASE COUNT          340 a          840 c          817 g          408 t
ORIGIN      
        1 ggtgcgcgag cgctcggcga gcagtgagat ccgcagcacc acggtgtccg cgccgccgcg
       61 tgcgaccggc tcgcggtagg tgcgctccgc gacagtctcg agcgcctcct cggcgcgacg
      121 cgcggcctcg gcgtccgcgc gctcggtcgc ggccttcgcc accgtcgcgc gtcgcttctc
      181 gagcaggaac gcgcggccgg gcgtgctcga cgcgatctcg cggtcgagct ccgtcacctc
      241 gtcggtgccg agggtcgtct cggactcccg tgtcagcgtg acgatccact cggcgcgccc
      301 gttcagcgcg acgagccggc gcgcgcgctg ctcgacgtcc tcgcgcagca tctcgcgcac
      361 gcgcacgtcg tcgcgataca gggtgccgaa cgagagcggg agcaccgtgc ccgcgatgtc
      421 gaggacccgc tcgttgaccg cctggtgggc cgccgcccgc ggcgtcagcc agtcgagatc
      481 gcggatccgc tcgttcaggg tctcggcggc gtactccgtg tcggagagct cgctcgtcgt
      541 cgccgcgaac ggaccctcgg cgatggtgcg gaccggagcg tcgtcgatgc cggtgagcgc
      601 cgctccatcg acgagcggcg cgacgctggc cgggacgatg ccgtacacgt agcgcagtcc
      661 gctcatcgca tctcttccgg aagatcgatg acgacatggc gctgccgctc gacggcggcg
      721 cccgcgtcgc ccgcctcctc gcgtgcccgc gccttgtggt gctcgcggat ctcacgcagg
      781 cgcaggagca gctcggcctc gcgcacgcgg aactcctcct cggtaagccg cccctcctcg
      841 tacgcctggt tgtcgaggat cagctgctcg cgcacggggc cttcgtccca catctccttg
      901 tccgcgacct cggcgacctt ctcgaggcaa tagcggatac ccgcgaccgg ggcgctgatc
      961 gggagcgtga gcagacggag gagcacgcta gaccccggcg ctccgctcca gctgcagtcg
     1021 gatggtgacg aagttgtagg ggggccacgg cccggtgtac gtgaacttga gcttcccttc
     1081 gtaccgccga ccgatctcct gcaccttctt gtcgaacatg tcggccttgt cgcgttcgac
     1141 gaggaaggcc gcgttcatga tcatccggtc gccgatcacc ttgttggagc gtgaggcgat
     1201 cgccgactcg cggagctcgt cgtagatctc gcgcacgaac gtctcggact tctctgcgag
     1261 cgcctgctcg acgaggcggc cgagctgcat gcgcgcgaag taggtcgact gggcctgctc
     1321 gccttcgatc tgcgccttga gacggcggat ctcctcgtag tcgcgctcga cgtcggcgag
     1381 caccccgtcg cggtcccaat tcaccttgag gccgaactcg agcttgtcct tcatcttgcg
     1441 cagcacgtcg cggagcgctt cgtaggtgtc cttcaggaac tcgacgatgt cgtcctcggt
     1501 cttgaagagc gtgccgaagc tcatcggcac gggtgtgaac gcgttgtcga tcatgacgac
     1561 ctcgttcacg tgctcgtgtg tgagcgcgtt ctcgcgggtg gggtcgtaga ccatgagtgg
     1621 cgtccggctc acgacggcgg cgagctcgcg atggtggacc gtgaagacct cgtcgccgcg
     1681 cccgccgatg ccgatctttc cgaaggcgcg cggctccgcg ctttcgatga tgcagtagac
     1741 gtaaatgccc tcgcccacct gctgattctc cgagggtcgc gccaccatcg cgtccaagaa
     1801 gtgcgactgt agagaagcga gccacgtgtc gtcacgtgaa gcgctcgctt ggatggcagc
     1861 tcgccgtacg ctggttgacg agaatgacgt ctgccgggcc cctagctcaa cggcagagca
     1921 gaggactttt aatcctttgg ttccgggttc gaatcccggg gggctcacac ggtcgcctgc
     1981 ccgctcaacg cctgcccgct caacgcctgc cggcggcgcc atcagaccgc tacggctgaa
     2041 cgccccggcc ggtcgctcgc cgcgggcggt acccgctcgt gaggtcgtcc gctccgctca
     2101 gtcgagtcgt gggacgcggc ggcccggcgc cgccgatgcg gcgcgcaccc agagtgcggc
     2161 gttgaccgcg gccccgagcg agacgaacgc gagcgccgcg ccggtgacga cctcgcgctg
     2221 accctggaac gagaaccatg gcggagtcag caccgcgatc tgaaatgccc agtacgccag
     2281 cgcgatcgtg tgcgcccaga gccactcgac gcgcgagtgg cggcgcaggt tcgggacgag
     2341 taccgcgagg ccgatcgcgg cctcggcgag gtggccgatg ccgctgacga actgcacctc
     2401 ggtct
//
LOCUS       DASTZR010000030         2399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_158832, whole genome shotgun sequence.
ACCESSION   DASTZR010000030 DASTZR010000000
VERSION     DASTZR010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158832"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VFV20_00655"
     CDS             complement(<1..220)
                     /locus_tag="VFV20_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286888.1"
                     /translation="MTARHPNAPISDDLLGQDAVRESVRGRYREVIGRQTEVAAQLYS
                     SEELALIPREAIEAALGVGDPIARAALAP"
     gene            complement(217..429)
                     /locus_tag="VFV20_00660"
     CDS             complement(217..429)
                     /locus_tag="VFV20_00660"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEU5286889.1"
                     /translation="MERLWTYLDRALDRTREEELEQHLGLCRHCCGELEFAKQVRAKL
                     KTTADAPLTPTAKERLEAFVDRLDQR"
     gene            complement(447..1040)
                     /locus_tag="VFV20_00665"
     CDS             complement(447..1040)
                     /locus_tag="VFV20_00665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU5286890.1"
                     /translation="MSVHAAAESTGSSFVTLVEPHLDYLYAFAFRLSGDRAGAEDLVQ
                     DALVRAFRARDALRNREQPRSWLTKVLLSASADRARKDAPREHDVPLEDERFSLFDTL
                     AEEDPFPYSDRLHLDFLDLFDDEKLTEALQRITPTHRAALLLAYVYGYKAREIADITG
                     TTTGTVLARLHRGRKQLERALWDYAKQRGLLKGEDRR"
     gene            1135..1701
                     /locus_tag="VFV20_00670"
     CDS             1135..1701
                     /locus_tag="VFV20_00670"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008164265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEU5286891.1"
                     /translation="MATATPAITNGVNVTQLVDTIELIKRDPGLATFRFRSQTHWQGG
                     GRCETRIDGFYGAGQEMTRPTTHRLTGDEPPVLLGGDAAPNAVEAVLHALAACISVGY
                     VYNAAARGIAIESLRFDAEGELDLRAFLGLSETVRPGFKSITFTATVKADATRRDLEE
                     LCEYVQRTSPVLDIIRNPVPVTIGLVTE"
     gene            complement(1752..2000)
                     /gene="gvpJ"
                     /locus_tag="VFV20_00675"
     CDS             complement(1752..2000)
                     /gene="gvpJ"
                     /locus_tag="VFV20_00675"
                     /inference="COORDINATES: protein motif:HMM:NF012944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0012506 - vesicle membrane [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpJ"
                     /protein_id="HEU5286892.1"
                     /translation="MALVDVLERVLAKGIVIAYQADVSVVGLRVIEVDGCAVVMALET
                     YVEMTEPPSTEAESSEALMAAVDEYLHRLAPSDPTPPM"
     gene            2161..>2399
                     /locus_tag="VFV20_00680"
     CDS             2161..>2399
                     /locus_tag="VFV20_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286893.1"
                     /translation="MATLLPRPNPLRAGIRALLEAKQLASLGKHDDAIAALEAAYAAG
                     CRYKKEWLTSDRDLAPLATIPAFGDLVSRANARYE"
BASE COUNT          360 a          851 c          816 g          372 t
ORIGIN      
        1 cgggggccag cgcggcccgc gcgatcggat cgccgacgcc gagcgcggcc tcgatcgctt
       61 cccgtgggat cagcgcgagc tcctcgctcg agtagagctg cgcggcgacc tcggtttggc
      121 gaccgatcac ctcgcgatac cgcccgcgga ccgactcgcg gacggcgtcc tggccgagca
      181 ggtcgtccga gatcggcgcg ttcggatgcc gagcggtcac cgctggtcga gccggtcgac
      241 gaacgcttcg agccgctcct tggcggtcgg cgtgagcggc gcgtcggctg tcgtcttcag
      301 cttcgcgcgc acctgcttcg caaactcgag ctcgccgcag caatgacggc agagcccgag
      361 atgctgctcc agctcctcct cgcgcgtccg atcgagcgcg cgatccagat acgtccagag
      421 ccgttccacg gcctcacgac acgcgatcat cgccggtcct ctcccttcag cagcccccgc
      481 tgcttcgcgt agtcccagag cgcgcgctcg agctgcttgc ggccgcggtg cagccgcgcg
      541 agcacggtgc cggtcgtcgt gccggtgatg tccgcgatct cgcgtgcctt gtagccgtag
      601 acgtaggcga ggaggagcgc ggcgcgatgc gtcggagtga tgcgctggag cgcttccgtc
      661 agtttctcgt cgtcgaagag atcgaggaag tcgagatgga gccgatccga ataggggaac
      721 gggtcctcct ccgcaagcgt gtcgaagagc gagaagcgct cgtcctccag cggcacgtcg
      781 tgctcgcgcg gggcatcctt ccgggcgcgg tcggccgatg ccgacagcag caccttcgtc
      841 agccagctcc gcggctgctc gcgattgcgg agcgcgtcgc gcgcgcggaa cgcgcggacc
      901 agcgcgtcct gcaccaggtc ctccgcgccg gcgcggtcgc cggacagcct gaaggcgaac
      961 gcgtagaggt agtcgaggtg cggctcgacc agggtcacga acgaggatcc ggtcgattcc
     1021 gccgcggcgt gcacgctcat aagacgcgca attgtgtccc ttttattgca gcgtgccggc
     1081 aatacttggg gacgacggcg gcgtctttgt ctgcacagcc ttaggaggga cgacatggcc
     1141 accgccacac ccgcgatcac caacggcgtc aacgtcaccc agctggtcga cacgatcgag
     1201 ctcatcaaga gggaccccgg tctcgccacg ttccgcttcc gctcgcagac gcactggcag
     1261 ggaggcggtc gctgcgagac gcgcatcgac ggcttctacg gcgccggtca ggagatgacc
     1321 cgtccgacga cccaccggct gacgggagac gagccgccgg tgctgctcgg tggcgacgcc
     1381 gcgccgaacg ccgtcgaggc ggtgctgcac gcgctcgccg cgtgcatctc ggtcggctac
     1441 gtgtacaacg ccgcggcccg cggcatcgcc atcgagtcgc tgcgcttcga tgcggagggc
     1501 gagctcgatc tgcgcgcgtt cctcggtctg tccgagacgg tccggcccgg gttcaagagc
     1561 atcaccttca ccgcgacggt gaaggccgac gcgacgcgtc gcgacctcga ggagctctgc
     1621 gagtacgtgc agcgcacctc gccggtgctc gacatcatcc gcaatcccgt gcccgtcacg
     1681 atcggactcg tgaccgagta gccgttcacg ttggggcgcg cccggcgcgg gcggcgaggg
     1741 cgcgcctcgg cttacatcgg cggcgtgggg tcgctcggcg cgaggcgatg cagatattcg
     1801 tcgaccgccg ccatgagcgc ctcgctgctc tccgcttcgg tggagggtgg ctcggtcatc
     1861 tcgacgtagg tctcgagcgc catcacgacc gcgcacccgt cgacctcgat gacgcgaagt
     1921 ccgacgaccg acacgtccgc ctggtacgcg atcacgatcc ccttggccag cacccgctcc
     1981 aggacgtcga caagcgccac gtcagatcgc tgtgtggctc gctccccttc gcgcatgggc
     2041 gaaatgccta caccgcgcgt gccacaaggc cgtgcccgcg cccgtatccc ggcgcgagcg
     2101 accacctgtt accgagctga cgtgaaggtc ggctgcgtgc cagtcccgca caatcagggc
     2161 atggccaccc tcttaccgcg cccgaatccc cttcgcgccg ggatccgcgc cctgctggag
     2221 gcgaagcagc tcgcgtcgct gggcaagcac gacgacgcga tcgcggcgct cgaggccgcg
     2281 tatgccgcgg gctgccgtta caagaaggag tggctcacga gcgaccgcga tctcgcgccc
     2341 ctggcgacca tcccggcgtt cggggacctc gtgtcgcgcg cgaacgcgcg gtacgagga
//
LOCUS       DASTZR010000031         2362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_53882, whole genome shotgun sequence.
ACCESSION   DASTZR010000031 DASTZR010000000
VERSION     DASTZR010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53882"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="VFV20_00685"
     CDS             <1..1062
                     /locus_tag="VFV20_00685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5286894.1"
                     /translation="RAIAAETEQPAIAAQMVMSANALGGRSTLIRRADGSLVAGDPLP
                     DETALRVRDLKLPAVLPVADASGRRVAVLPAGPGLALIAAFPAGELDDPVLAALGSIG
                     TVGGAALGAATERDRLEEERRELLALADRLRSDLRARDDAVASTVHELRTPLTSVSAY
                     GQLIARQLQSALQQLAQIDRLIGDLRSDATMGLALTEVNVATEASEAVRRQRVLNRAD
                     VDLRIGAGAPFVVRADAGRLAQVLDNVLGNAVKFSPPDGRIDLSVDRVDGEVVISISD
                     EGPGLATEDLEHIFERYYRARTTSTRVPGLGIGLAVSREIVQAHGGRIWAESPGEGRG
                     STFKIALPTATADVSAKVR"
     gene            complement(1055..1588)
                     /locus_tag="VFV20_00690"
     CDS             complement(1055..1588)
                     /locus_tag="VFV20_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011600502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEU5286895.1"
                     /translation="MTLTVERPSIEPAPATDEEKATGGFVILAWSGELDKIWPTLILA
                     NTAAALGKPVTIFFTFWGLFPLVKDDRGITGANWMQKMLSTMNRGGIKNLKLSKMNFM
                     GMGPAMMKSLATRYNVSSPQELLDTAKEMGVRLIPCQMTMDLMGLTKDDLIDGLEEPA
                     GAATALAAAEGATTLFI"
     gene            complement(1593..1829)
                     /locus_tag="VFV20_00695"
     CDS             complement(1593..1829)
                     /locus_tag="VFV20_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEU5286896.1"
                     /translation="MTLAIARTLDVKGLSCPLPIVKTAQALKQLAAGDLIEVLATDPG
                     SVKDFAAWAKTTGNEIVETSAEGGVFRFVLRKKG"
     gene            complement(1842..>2362)
                     /locus_tag="VFV20_00700"
     CDS             complement(1842..>2362)
                     /locus_tag="VFV20_00700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEU5286897.1"
                     /translation="RKGIELSTFFNVESVDEPAHTVTSIEGETFGYDLLVLVPPHRGA
                     AVVDASGLGDSGGWIPTDRNTLQHEKHERIFAIGDATNLPISKSGSTAHFEAPVVASR
                     IASLIHGTAPKNAYEGRVMCFLETGGGRATSLRFDYAHPPVPPQPSRLWHWAKWAFNR
                     AYWITVPQGRVG"
BASE COUNT          338 a          815 c          826 g          383 t
ORIGIN      
        1 cgcgccatcg ccgccgagac cgagcagccc gcgatcgccg cccagatggt gatgtccgcg
       61 aacgccctcg gtggccgatc gacgctcatc cgacgcgcgg acgggtcgct cgtcgcgggc
      121 gatccgctgc cggacgagac ggccctgcgc gtccgcgatc tgaagcttcc cgccgtcctg
      181 ccggtcgccg atgccagcgg ccgccgcgtc gcggtgcttc cggcgggtcc cggtctcgcg
      241 ctcatcgcgg cgttccccgc gggcgagctg gacgaccccg tccttgccgc gctcggcagc
      301 atcggcacgg tgggcggtgc ggcgctcggc gccgcgaccg agcgggacag gctggaggaa
      361 gagcgccgcg agctgctggc gctcgccgac cggctgcgca gcgaccttcg cgcacgcgac
      421 gacgccgtcg cgtcgaccgt ccacgagctg cgtacgccgc tgacctcggt gagcgcctac
      481 ggtcagctga tcgcacggca gctgcagtcg gccctgcagc agctcgccca gatcgaccgt
      541 ctcatcgggg acctgcgcag cgacgcgacg atggggctcg ccctgaccga ggtgaacgtc
      601 gcgacggagg cgtccgaggc ggtgcggcgg cagcgggtcc tgaaccgtgc cgacgtcgac
      661 ctgcgcatcg gcgcgggcgc gcccttcgtc gttcgcgcgg acgccgggag gctcgctcag
      721 gtgctcgaca acgtcctcgg gaacgcggtg aagttctcgc cgccggacgg ccggatcgac
      781 ctctcggtcg accgcgtgga cggtgaggtc gtcatctcga tcagcgacga gggccccggc
      841 ctcgcgacgg aggacctgga gcacatcttc gagcgctact accgagcacg caccacgagc
      901 acgcgcgtac ccgggctggg catcggtctc gccgtatcac gagagatcgt ccaggcgcac
      961 gggggccgca tctgggccga gagtccgggc gagggccgcg ggagcacctt caagatcgcg
     1021 ctgcccacgg ccaccgccga cgtctccgcg aaggtcagat gaagagcgtc gtcgcaccct
     1081 cggccgcggc gagcgcggtg gcggctccgg ctggctcctc gagtccgtcg atgaggtcgt
     1141 ccttggtcag ccccatcagg tccatcgtca tctggcaggg gatgagccgg acgcccatct
     1201 ccttggcggt gtcgaggagc tcctgcggac tcgacacgtt gtagcgcgtc gcgagcgatt
     1261 tcatcatcgc cggccccatg cccatgaagt tcatcttcga gagcttcagg ttcttgatgc
     1321 cgccgcggtt catcgtggaa agcatcttct gcatccagtt cgcaccggtg atgccgcggt
     1381 cgtccttcac gagtggaaag agaccccaga acgtgaagaa gatcgtgacc ggcttgccga
     1441 gcgcggccgc ggtgttcgcg aggatgagcg tgggccagat cttgtccagc tcgccgctcc
     1501 aggcgaggat cacgaacccg ccggttgcct tctcttcgtc ggtggcgggc gctggctcga
     1561 tgctcggtcg ttcgacggtg agcgtcacgg cgtcacccct tcttgcggag gacgaagcgg
     1621 aacacgccgc cctccgctga tgtctcgacg atctcgttgc cggtcgtctt cgcccacgcg
     1681 gcgaagtcct tcaccgaccc cggatcggtc gcgaggacct cgatgaggtc gcccgcagcg
     1741 agttgcttga gtgcctgcgc ggtcttcacg atggggagcg ggcacgagag ccccttcaca
     1801 tcgagcgtgc gcgctatggc gagcgtcatg tcgtcggcct cctatcccac acggccctgc
     1861 gggaccgtga tccagtacgc gcggttgaac gcccatttcg cccagtgcca gagacgcgac
     1921 ggctgcggcg gcaccggtgg gtgtgcgtag tcgaagcgga gcgaggtggc gcgtcctccg
     1981 cccgtctcga ggaagcacat gacacgcccc tcgtaggcgt tcttcggagc tgtgccatgg
     2041 atgagcgacg cgatgcggct cgcgaccacc ggcgcctcga agtgcgcggt cgagccgctc
     2101 ttcgagatgg ggaggttcgt cgcgtcgccg atggcgaaga tgcgctcgtg cttctcgtgc
     2161 tggagcgtgt tgcgatcggt ggggatccat cccccgctat cgccgagccc ggacgcatcg
     2221 acgaccgccg caccgcggtg cggcggcacg agcacgagca ggtcgtaccc gaacgtctcg
     2281 ccttcgatgg acgtcacggt gtgggccggc tcatccacgg actcgacgtt gaagaacgtg
     2341 ctgagctcga tgcccttccg gg
//
LOCUS       DASTZR010000032         2407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_132932, whole genome shotgun sequence.
ACCESSION   DASTZR010000032 DASTZR010000000
VERSION     DASTZR010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132932"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VFV20_00705"
     CDS             <1..309
                     /locus_tag="VFV20_00705"
                     /inference="COORDINATES: protein motif:HMM:NF013614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HEU5286898.1"
                     /translation="YQECRNMLLSDKAGADPIPVLEILTNDVARCGHGATAGALEEDE
                     LFYVMSRGLDRIGAESLLVRGFFQRVVAAIPEPQVRSKVLAALAPRIGRIAELDAAAA
                     "
     gene            329..670
                     /locus_tag="VFV20_00710"
     CDS             329..670
                     /locus_tag="VFV20_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEU5286899.1"
                     /translation="MSNFVPVAHESDVAPGEVRVVEAGGRSLALGNCGGGTWGAIDNV
                     CTHDGGVLGEGELEDCLVECPRHGARFDLLTGEVKALPAVFPVNAYPVRVVDGQVEVD
                     LGVVTKELEIG"
     gene            complement(709..1662)
                     /locus_tag="VFV20_00715"
     CDS             complement(709..1662)
                     /locus_tag="VFV20_00715"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEU5286900.1"
                     /translation="MRRLLIVPLLLSACAGPQGVAPSPSPTELERPAVSATATAPLTP
                     SPTAAATQSTSPSRTPAPASATPTRTPALSTASAAPSVPGGEAYCTVADVLTPNRAYA
                     EHPRTYLDWTYALPQSYVPPDLVDAVSGVDAAPSPFAVEAVGAAEVMARRGDPAYSTL
                     LADAKGSAIRRVAYADLVALRDAASQAGARLVLLSTYRSYALQEITFDYWVKVGGYAQ
                     ALRTSARPGHSEHQLGTVIDFGDGSAAPWEYADWATTPTGAWLKQHAAEFGFVMSFPK
                     GGTPVTCYDYEPWHYRWVGRDVAVRIVAADTPLRVFQAGLR"
     gene            complement(1668..>2407)
                     /locus_tag="VFV20_00720"
     CDS             complement(1668..>2407)
                     /locus_tag="VFV20_00720"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU5286901.1"
                     /translation="GLTQRPLGDVSRDAESYARSRPPTWGVAAIVPSRGIVYAENADT
                     QVPTASIVKVLVLLTVLEQARQDHRPASDTELALLWPMITESDNDATSQLWEDIGRGQ
                     AVASYLRGIGVSGFTPDPATSWGVSFVSARAMATTLGKLLAGEILDAPSRTLALRMLD
                     GVVPQQRWGVTAGTSSEAGDRVGVKNGWYPGEEGWRVNSVGVVRPAGAAPYALAIVTD
                     GRATMREGIETIEGIAGPMNAALRPKG"
BASE COUNT          317 a          866 c          871 g          353 t
ORIGIN      
        1 taccaggagt gccgcaacat gctcctctcc gacaaggccg gcgcggaccc catcccggtg
       61 ctcgagatcc tcaccaacga cgtggcgcgc tgcggtcacg gcgcgacggc cggcgccctc
      121 gaggaggacg agctcttcta cgtcatgtcg cgcgggctcg atcgcatcgg tgcggagtcg
      181 ctcctcgtgc gcggcttctt ccagcgggtc gtcgccgcga tccccgagcc gcaggtgcgc
      241 tcgaaggtcc tcgccgcgct cgcgccgcgc atcgggcgca tcgccgagct cgacgccgcg
      301 gccgcctgat gcgcagcggg tgtggcgcat gagcaacttc gtgcccgtcg cgcacgagtc
      361 cgacgtcgcg ccgggcgagg tccgtgtcgt cgaggccggg ggccggagcc tggcgctcgg
      421 gaactgcggc ggcggcacct ggggcgccat cgacaacgtc tgcacccacg acggcggcgt
      481 gctcggcgag ggcgagctcg aggactgcct cgtcgagtgc ccgcgtcacg gcgcgcgctt
      541 cgacctcctg accggtgagg tgaaggcgct tcccgccgtg ttcccggtca acgcgtaccc
      601 cgtgcgcgtg gtggacggcc aggtcgaggt ggacctcggc gtcgtgacga aagagctcga
      661 gatcggctga gcgcgctcac tcgctgcgcg tgcttcccgc gcggatccct agcgcagacc
      721 ggcctggaac acgcgtagcg gcgtgtcggc cgcgacgatc cgcacggcga catcgcggcc
      781 cacccagcgg tagtgccagg gctcgtagtc gtagcacgtg accggtgtgc cgcctttcgg
      841 gaagctcatc acgaacccga attccgccgc gtgctgcttg agccaggccc cggtcggcgt
      901 cgtcgcccag tccgcgtact cccatggcgc cgcgctgcca tcgccgaagt cgatgaccgt
      961 ccccagctgg tgctcggagt gtcccggccg cgcgctcgtg cgtagcgcct gcgcgtagcc
     1021 gccgaccttc acccagtagt cgaaggtgat ctcctggagc gcgtacgagc gatacgtgga
     1081 gaggagcacc agtctcgcgc cggcttgcga cgcagcgtcg cgcagcgcga cgaggtcggc
     1141 gtacgccacc ctgcggatcg cggagccctt cgcatccgcg aggagcgtcg aatacgcggg
     1201 gtcgccacgc ctggccatca cctcggccgc gccgacggcc tcgaccgcga acggcgaggg
     1261 tgcggcatcc acgccgctca cggcgtcgac gaggtcgggc ggcacgtagc tctggggcag
     1321 ggcgtacgtc cagtccagat acgttcgcgg atgctcggcg taggcgcgat tcggtgtgag
     1381 cacgtccgcg accgtgcagt acgcctcacc gccggggacg ctcggcgcgg ccgacgccgt
     1441 gctcagggcg ggcgtgcgcg tcggtgtggc gctcgcgggt gccggcgtgc ggctcgggct
     1501 cgtgctctgc gtcgcggccg cggtcgggct cggtgtgagg ggcgcggtcg cggtcgcgct
     1561 cacggcgggc cgctcgagct ccgtcggcga cggcgacggg gcgacacctt gcggcccagc
     1621 gcaggcggac agcagcagcg gcacgatgag cagccgtcgc atcgcgctca tcccttcgga
     1681 cgcagcgccg cgttcatcgg acccgcgatg ccctcgatcg tctcgatgcc ctcgcgcatg
     1741 gtcgcgcgtc cgtcagtgac gatcgcgagc gcgtacggag cagcgccggc tggccgtacg
     1801 acaccgacgc tgttcacgcg ccacccttcc tcgccgggat accagccgtt cttcacgccg
     1861 acccgatcgc ccgcctccga cgaggtgccc gcggtgacgc cccagcgctg ctgcggcacg
     1921 acgccgtcga gcattcgcag cgcgagggta cgtgacggcg cgtcgaggat ctcgccggca
     1981 agcagctttc ccagcgtcgt ggccatcgca cgtgcggaca caaagctcac gccccacgac
     2041 gtcgcggggt cgggtgtgaa gccggagacg ccgatgccgc gcaggtacga ggcgacggcc
     2101 tggccgcgac cgatgtcctc ccacagctgg gacgtggcgt cgttgtcgct ctcggtgatc
     2161 atcggccaga ggagcgcgag ttcggtgtcc gacgccggtc ggtggtcctg ccgcgcctgc
     2221 tcgaggaccg tgagcagcac gagcaccttc acgatgctgg cggtcgggac ctgcgtgtcg
     2281 gcgttctccg cgtacacgat gccgcgcgac ggcacgatgg ccgcgacgcc ccacgtcggt
     2341 ggcctcgatc gcgcgtacga ctcggcgtcg cgagagacgt cgccgagcgg acgctgcgtc
     2401 agcccgg
//
LOCUS       DASTZR010000033         4032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_994578, whole genome shotgun sequence.
ACCESSION   DASTZR010000033 DASTZR010000000
VERSION     DASTZR010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994578"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VFV20_00725"
     CDS             <1..363
                     /locus_tag="VFV20_00725"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013043339.1"
                     /note="involved in lysine biosynthesis; DAP epimerase;
                     produces DL-diaminopimelate from LL-diaminopimelate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEU5286902.1"
                     /translation="DLAAVDARVLGPAIERHPAFPAKTNVQFAAVRSRSHLAIEIWER
                     GAGYTLASGTSSCAAAAAAHRKGLVDRDLTVTMPGGDLEVTVGDGYAMRLRGPVEEVM
                     TGDLSPDLLAKIRAASPS"
     gene            complement(302..802)
                     /locus_tag="VFV20_00730"
     CDS             complement(302..802)
                     /locus_tag="VFV20_00730"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5286903.1"
                     /translation="MRIRRATEADAPSVARVLREAFAEVDRLYTRDAYAATAPAHAEI
                     ARRLAEGPTWVAEDEGAVVGTVAAAASERGVYVRSMAVAPAARGRRIALQLMRQLEIF
                     AVSKDAPRLYLSTTPFLFDAIRLYEAFGFRRTGEPPHDLHGTPLFTMAKPLVSSRADP
                     GSGPRS"
     gene            820..1782
                     /locus_tag="VFV20_00735"
     CDS             820..1782
                     /locus_tag="VFV20_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEU5286904.1"
                     /translation="MSAPPTLVDVLAARRFLEPYLRPTPLIQRPAVSEAIGIDVWLKC
                     ESLLPTGAFKVRGGLNLVGRDPTAKRGVYGASTGNHGQSLAYAGKVFGVPVTIFVPHD
                     ANPLKLAAMRANGATVVEHGVDFDEARVECERRAKAEGVRYVHSGNEPYLIAGVGTAT
                     LEVLLDLPTADVLIVPIGGGSGASAACVVAKALNPRVEVIGVQSDGAPAAYLTWREHE
                     PRETERVNTFADGLATRTTFAMPQAILRDMLDDFVLVSDDDLYRAMRVLLVEGHLVAE
                     GAGAAPVAAARKIRDRLAGKTVVCWVSGGNATAASLERALAMPS"
     gene            complement(1779..2201)
                     /locus_tag="VFV20_00740"
     CDS             complement(1779..2201)
                     /locus_tag="VFV20_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286905.1"
                     /translation="MWTWLVVGLLVLQAHFAISYIVPIDRANLDGWLPSLISWVFPWG
                     QGDRGLLNASGSSEGFALGFFIAAAATLLSLGAILAVFGLVVPHDWWKALTIAGAVVS
                     IVLLLGFFEPHKVVPIAFEAAVIVAIVTRWAPLTEAAA"
     gene            2353..3507
                     /locus_tag="VFV20_00745"
     CDS             2353..3507
                     /locus_tag="VFV20_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016576215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5286906.1"
                     /translation="MDFDLSPEQEQIRKLAHDFADNEIAPGARERDRGEIFPWDVLKK
                     MAPIGLLGAPVPEEYGGIGADMISHAIITEEIGRADSSVRTTLSVQISLVELTILRWG
                     TEEQKRAWLPRLCSGEIIGCFGLTEPAVGSDATKLQSTARRDGNDWVINGRKMWISNG
                     SVSKLALVFANADPSKGHKGITAFLLDREKSPYGSAGLHHKLGLRSSDSSELILENVR
                     VPDSMRLGEVGEGFKIAMSSLDSGRYSVAAGAVGQAQAAIDASVAYSLQRKSFGKPIA
                     SHQLVQEMIADMIVETEAARLLVWRAGHLKNKGVPSTRETSIAKLYAGEIAQRACDNA
                     IQIHGGYGFSDEFPVERLWRDSRVNRLYEGTTQIQKLIIGRFATGIDAIG"
     gene            3598..3909
                     /locus_tag="VFV20_00750"
     CDS             3598..3909
                     /locus_tag="VFV20_00750"
                     /inference="COORDINATES: protein motif:HMM:NF022535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2621 family protein"
                     /protein_id="HEU5286907.1"
                     /translation="MEWTDSADNLLTALARMVPETLRALASAAARDEAETIAQDRSSD
                     AVEVDDVMRGWIRTTPPEQRNSLVAVIEDLGLDAERYADDLEDAEGWEDADAERDPDG
                     E"
BASE COUNT          674 a         1408 c         1407 g          543 t
ORIGIN      
        1 gatctcgcgg ccgtcgacgc gcgcgtcctc ggtccggcga tcgagcggca cccggcgttc
       61 cccgcgaaga cgaatgtcca gttcgccgcg gtgcgttccc gctcgcatct cgcgatcgag
      121 atctgggagc gcggcgccgg atacacgctc gcgtccggca cgtcgagctg cgccgcggcg
      181 gcggcggcgc accgcaaggg cctcgtcgat cgcgacctca cggtcacgat gcccggcggc
      241 gatctcgagg tgaccgtcgg cgacggctac gcgatgcggc tgcgtggtcc ggtggaggag
      301 gtcatgaccg gggacctgag cccggatctg ctcgcgaaga tacgagcggc ttcgccatcg
      361 tgaacagcgg cgtcccgtgc aggtcgtgcg gcggctcgcc ggtgcgacgg aagccgaacg
      421 cctcgtagag gcggatcgca tcgaagagga acggcgtcgt gctgagatag aggcgaggcg
      481 cgtctttgga gaccgcgaag atctcgagct gccgcatcag ctgcagcgcg atccgccggc
      541 cgcgggccgc gggcgccacc gccatgctgc gcacgtacac gccgcgctcg ctcgcagccg
      601 ccgcgaccgt gcccacgacg gcgccctcgt cctccgccac ccaggtcgga ccctctgcca
      661 ggcgtcgcgc gatctccgcg tgggcgggcg cggtcgccgc atacgcgtcg cgcgtataga
      721 gacggtcgac ctcagcgaag gcctcccgca gcacacgcgc cacgctcggg gcgtcggcct
      781 cggtcgcgcg cctgatccgc accgggatag tctgccggtg tgagcgcgcc cccgacgctc
      841 gtcgacgtcc tcgccgcccg acgctttctc gagccgtacc tgcgcccgac accgctgatc
      901 cagcgccccg cggtcagcga ggcgatcggg atcgacgtgt ggctcaagtg cgagagcctg
      961 ctgcctacgg gcgcgttcaa ggtgcgcggc gggctgaacc tcgtcggtcg cgacccgacg
     1021 gcgaagcgcg gggtctacgg cgcgtcgacg ggaaaccacg ggcagtccct cgcgtacgcc
     1081 ggcaaagtct tcggcgtgcc cgtgacgatc ttcgttccgc acgacgcgaa tccgctcaag
     1141 ctcgccgcga tgcgcgcgaa cggggcgacg gtcgtcgagc acggcgtcga cttcgacgaa
     1201 gcgcgcgtgg agtgcgagcg ccgcgcgaag gccgagggcg tccggtacgt ccactccggc
     1261 aacgagccgt atctcatcgc gggcgtcggc acggcgacgc tcgaggtcct gctcgacctt
     1321 ccgacggcgg acgtcctcat cgtgccgatc ggcggcggca gcggcgcatc cgcggcgtgc
     1381 gtggtcgcga aggcgctgaa tccgcgcgtc gaggtgatcg gcgtccaatc ggacggtgcg
     1441 ccggccgcgt acctcacgtg gcgcgagcac gagccgcgcg agaccgagcg cgtgaacacg
     1501 ttcgccgacg gtctcgcgac gcgcacgacc ttcgcgatgc cgcaggcgat cctgcgcgac
     1561 atgctcgacg acttcgtgct cgtgtccgac gacgacctct accgcgcgat gcgcgtcctg
     1621 ctcgtcgaag gacatctcgt cgccgagggc gccggggccg cgccggtcgc ggcggcacga
     1681 aagatccgcg accgcctcgc cgggaagacc gtcgtctgct gggtcagcgg cgggaacgcg
     1741 acggcagcgt cgctcgagcg cgcgctcgcg atgccgtcct aggcggccgc ctcggtcaaa
     1801 ggcgcccacc gcgtcacgat cgccacgatg acggcggcct cgaacgcgat gggcacgacc
     1861 ttgtgcggct cgaagaagcc gagcagcagc acgatcgaga cgaccgcgcc ggcgatcgtg
     1921 agcgccttcc accagtcatg cggaacgacg aggccgaaca ccgcgaggat cgcgccgagt
     1981 gacagcagcg ttgcggcggc ggcgatgaag aagccgaggg cgaatccttc gctcgacccc
     2041 gacgcgttca gcagtccgcg atcaccctgg ccccacggga acacccagga gatcaacgac
     2101 ggcagccagc cgtcgagatt cgcacgatcg atcgggacga tgtaggagat cgcgaaatga
     2161 gcctgcagca cgagcaagcc gacgacgagc caggtccaca tgtgacatcc tccgcgggcc
     2221 gggagtttgc gcagccgcga ccagcgttcc atcagcagga cgatgcgtgc tagcgggcaa
     2281 tgcttcctca cgcgaggggt agcggcagac gctgacgcga ccctgacatg tccgaattgc
     2341 taggctcgtc gcatggactt cgacctctcg cccgaacagg agcagatccg caagctcgca
     2401 catgacttcg cggacaacga gatcgcgccg ggcgccagag agcgcgaccg cggcgagatc
     2461 ttcccgtggg acgtcctgaa gaagatggcg cccatcggcc tcctcggcgc gcccgtgccg
     2521 gaggagtacg gcggcatcgg cgccgacatg atcagccacg cgatcatcac ggaggagatc
     2581 ggccgtgccg actcgtcggt gcggacgacg ctctcggtcc agatctcgct cgtcgaactc
     2641 acgatcctga ggtggggcac cgaggagcag aagcgcgcgt ggctgccgcg cctctgcagc
     2701 ggcgagatca tcggctgctt cggactcacc gagcccgccg tcggcagcga cgcgacgaag
     2761 ctgcagtcca cggcgaggcg cgacgggaac gactgggtca tcaacggtcg caagatgtgg
     2821 atctcgaacg gctccgtctc gaagctcgcg ctcgtgttcg cgaacgccga cccctcgaag
     2881 ggccacaagg gcatcaccgc gttcctgctc gaccgggaga agagcccgta cggaagcgcc
     2941 ggccttcacc acaagctcgg gctgcgctcg tcggactcga gcgagctcat cctcgagaac
     3001 gtgcgcgtgc cggacagcat gcgtctcggc gaggtcggcg agggcttcaa gatcgcgatg
     3061 tcgtcgctcg actccggccg ctactcggtg gccgccggcg cggtgggcca ggcgcaggcc
     3121 gccatcgacg cctcggtcgc gtactcgctc cagcgcaagt cgttcggcaa gccgatcgcg
     3181 tcacatcaac tcgtacagga gatgatcgcg gacatgatcg tcgagaccga ggccgcccgc
     3241 ctcctggtct ggcgagccgg tcacctgaag aacaagggcg tgcccagcac gcgcgagacg
     3301 agcatcgcga agctctatgc gggcgagatc gcgcagcgcg cgtgcgacaa cgcgatccag
     3361 atccacggtg gctacggctt cagcgacgag ttccccgtcg agcggctctg gcgcgactcg
     3421 cgcgtgaatc gcctgtacga gggcaccacg cagatccaga agctcatcat cggccgcttc
     3481 gcgacgggca tcgacgcgat aggctgagcc cactcgaaca gcatggagtg gactcgcgac
     3541 gagcgattca ctcgcgagga gcgggaccgc tgcccgagtg acaatgaggg gtaaagcatg
     3601 gagtggaccg actcggccga caatctgctc acggcgctcg cgcgcatggt ccccgagacc
     3661 ctccgcgcgc tcgcgagcgc ggccgcgcgt gacgaggcag agacgatcgc gcaggaccgc
     3721 tcgagcgacg cggtcgaggt ggatgacgtg atgcgcggat ggatccgcac gaccccgccg
     3781 gagcagcgga acagcctcgt cgccgtcatc gaggatctcg gtctcgacgc cgagcgctac
     3841 gccgacgacc tcgaggacgc cgagggctgg gaggacgccg acgcggagcg agaccccgac
     3901 ggagagtagg accggccacc cggcgccccg gcaggccatc gtccccatcg tcctcatcac
     3961 gctcgcggcg acgcccgccc tgctgacgcg catcggggtc atcgcgccgt cggtcgagct
     4021 cggcacgctc ta
//
LOCUS       DASTZR010000034         2300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_263646, whole genome shotgun sequence.
ACCESSION   DASTZR010000034 DASTZR010000000
VERSION     DASTZR010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263646"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VFV20_00755"
     CDS             complement(<1..534)
                     /locus_tag="VFV20_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5286908.1"
                     /translation="MTSTPSERDAKTARPITVLVADDSVLQRALVRFTLTSQGWAVAE
                     AATAEAAGELVRATRPDLVLMDITFGDTGSDGLQMCADLKADPSTAAIPIVMLTAHDD
                     PAVRAQASAAHADGFVTKPFGPLDLMNTVSALLSVPPVSPALGVYLVDAGAVDPTVLE
                     RALEEQRELSDRGAPKRL"
     gene            621..1313
                     /locus_tag="VFV20_00760"
     CDS             621..1313
                     /locus_tag="VFV20_00760"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5286909.1"
                     /translation="MAESTNGLRPVPAIDKAARVLRALAEGGRPQGISELARTLGVSK
                     GTLRDVLLTLDAYGFVVRDPDTRFRLGPELRTLADASTPDLRTIAQPYLVSLMESFGE
                     TAILGVVHDGKLEIAARAEPASDLHMTAPLGRRLPLDEGAHGQVLTKGVPVGYDDEEL
                     FAGVRAAAAPIVDARGRTAAFVMVVGFKQRVDLRTLRRIGERCAGAADAISRRIGAKP
                     GGAAPADRSASA"
     gene            1310..1777
                     /locus_tag="VFV20_00765"
     CDS             1310..1777
                     /locus_tag="VFV20_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU5286910.1"
                     /translation="MTKELLTLRVNGETHRVAAEPHHTLLEVLREELDLTGTKHGCEL
                     GECGACTVLVDGVPVLSCLTLPLEVRDAEVTTVEGLAKGPELHPLQRAFAEEGAAQCG
                     YCTPGMLLSAKALLDRTARPSRDEIASAISGNLCRCTGYTAIYEAVEKAARDV"
     gene            1770..>2300
                     /locus_tag="VFV20_00770"
     CDS             1770..>2300
                     /locus_tag="VFV20_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidase"
                     /protein_id="HEU5286911.1"
                     /translation="MSELSILGRRLPKTNSWAHLTGSARYADDIHLPRMLYGRLLRST
                     RPHARIKRIDVSRAQAHPGVVAIVTGADMPEKMGIMPSTQDETALAVGKVRYVGEPVA
                     AVAALDEDTAYEALSLIDVEYEDLEPIFTIEEALEREDVKIHEDSRRANVFKEVHLSF
                     GDVDAGFASADHVRDDW"
BASE COUNT          337 a          813 c          837 g          313 t
ORIGIN      
        1 gaggcgcttc ggtgcgccgc gatcgctcag ctcgcgctgc tcctcgagcg cgcgctcgag
       61 caccgtcgga tcgaccgcgc ccgcgtcgac gagatagacg ccgagcgcgg gtgagaccgg
      121 cggcaccgac aggagcgcgc tgaccgtgtt catgaggtcg agcggaccga acggcttggt
      181 gacgaagccg tccgcatgcg ccgcgctcgc ctgtgcgcgc accgcgggat cgtcgtgggc
      241 ggtcagcatg acgatcggga tcgcggccgt gcttggatcg gccttgaggt cggcgcacat
      301 ctggaggccg tcgctgccgg tgtcgccgaa cgtgatgtcc atcagcacga ggtccggtcg
      361 cgtggcgcgc acgagctccc cggcggcttc ggccgtggcg gcctcggcca ccgcccagcc
      421 ctggctggtc agcgtgaagc gcacgagcgc gcgctgcagc accgagtcgt ccgcgaccag
      481 gacggtgatc ggtcgcgcgg tcttcgcgtc gcgctcgctc ggggtgctcg tcaagcaagt
      541 ctcaacgacc ggcgccgggt ttccaagtgg cggcgcagta tcggcagcct cacggtcgtc
      601 gtaccatgac gcgtacgcgt atggcggaga gtacgaacgg ccttcggccg gtcccggcga
      661 tcgacaaagc ggcacgggtg ctccgtgcgc tcgccgaggg tggccgcccg cagggcatca
      721 gcgagctcgc gcggaccctg ggagtctcga aaggcacgct gcgggacgtc ctgctgacgc
      781 tggatgccta cggcttcgtc gtccgcgacc cggacacgcg cttccggctc ggcccagagc
      841 tgcgcacgct cgccgatgcg agcacccccg acctgcgcac catcgcacag ccgtacctcg
      901 tctcgctcat ggagtctttc ggcgagaccg cgatcctcgg cgtcgtccat gacggcaagc
      961 tcgagatcgc ggcacgcgcg gagccggcca gcgaccttca catgaccgcg ccgctcggcc
     1021 gccgcctgcc gctcgacgag ggcgcccacg ggcaggtcct gaccaagggg gtcccggtgg
     1081 gatacgacga cgaggagctc ttcgcgggcg tgcgcgccgc cgccgcgccg atcgtcgacg
     1141 cgcgcggccg caccgccgcg ttcgtcatgg tggtgggctt caagcagcgg gtcgacctgc
     1201 gcacgctgcg ccgcatcggc gagcgctgcg ccggcgccgc ggacgcgatc tcgcgccgca
     1261 tcggcgcgaa gccgggcggg gccgcgccgg cggaccggag cgccagcgca tgacgaagga
     1321 gctcctgacc ctgcgcgtga acggcgagac gcaccgcgtc gcggcggagc cgcatcacac
     1381 gctcctcgag gtgctgcgcg aagaactcga tctcacgggc acgaagcacg gctgcgagct
     1441 cggggagtgc ggcgcgtgca cggtgctcgt ggacggcgtg ccggtgctct cctgcctgac
     1501 gctcccgctg gaggtgcgcg acgccgaggt cacgacggtc gagggcctgg cgaaggggcc
     1561 ggagctgcat ccgctccagc gcgcgttcgc ggaagagggc gccgcgcagt gcggctactg
     1621 cacgccgggg atgctcctga gcgcgaaggc gctcctcgac cgcaccgcgc ggccgagccg
     1681 cgacgagatc gcgagcgcga tcagcggcaa tctctgccgc tgcaccggtt acacggccat
     1741 ctacgaggcg gtggagaagg cggcgcggga tgtctgagct ttcgatcctc ggccggcgcc
     1801 tgcccaagac gaactcgtgg gcccatctca cgggaagcgc gcgctacgcc gacgacatcc
     1861 acctgccgcg gatgctctac ggccggctgc tgcgctcgac acggccgcac gcacgcatca
     1921 agcgcatcga cgtctcgcgc gcgcaggcgc acccgggcgt cgttgccatc gtcaccgggg
     1981 cggacatgcc cgagaagatg ggcatcatgc cgtccaccca ggacgagacg gcgctcgcgg
     2041 tgggcaaggt gcgctacgtg ggcgagcccg tcgccgcggt cgcggcgctc gacgaggaca
     2101 ccgcgtacga ggcgctctcc ctcatcgacg tcgagtacga ggacctcgag ccgatcttca
     2161 cgatcgagga ggccctggag cgcgaggacg tgaagatcca cgaggactcg cggcgcgcga
     2221 acgtcttcaa agaggtccac ctctcgttcg gcgacgtgga cgcgggcttc gcgtccgccg
     2281 atcacgtacg cgacgattgg
//
LOCUS       DASTZR010000035         4535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_629247, whole genome shotgun sequence.
ACCESSION   DASTZR010000035 DASTZR010000000
VERSION     DASTZR010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629247"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="VFV20_00775"
     CDS             <1..650
                     /locus_tag="VFV20_00775"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEU5286912.1"
                     /translation="ICAERDRGGSFGSFDEFARRVGLKEEALRNLALVGAFDAFGEQR
                     RALLWRARDAHRTSPAFARPPLALPATDAPRLPILSERERVALDYRITGIPTGPQIMR
                     FYRDELTRRGVLSAIELADHAHGELVRIGGAIVVKQHPETAKGHVFLSLEDETGMANV
                     IIRPATYRRFKRVLDAYAAVVVAGVLQTVDGVISVLAARLDGLDLFVEMKAREWH"
     gene            807..2072
                     /locus_tag="VFV20_00780"
     CDS             807..2072
                     /locus_tag="VFV20_00780"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEU5286913.1"
                     /translation="MDIATDRRALDLLDSIFRVATELVRGERASLLLRDDETSDFVIA
                     RAHGLAEEVQRQVRVRSGVGIAGHVLASRQSLLVRGPQDAPIPISGDRYRSSSFISVP
                     VIVDDEPRAVLSVADRTDGLPFEQADLQTLEILAGHIGACLVQQEQGEALQRLAETDP
                     LTWLFNRRHFDKRLESETNRAMRAENLLALLMIDVDKFKTINDRFGHHVGDQVLKAVA
                     AAIKQAVRLYDVPTRYGGDEFAIILPESDTDVASRVARRILEKTEAAAIPGELRDAGM
                     HIELSIGVATFPRPLGDATALVEAADTAMYRAKEAGGGVRVWEHSFAEGPRGALRSGR
                     VALPPAPYLADPARLATRELQQLVPAALAQEWNAVVVGRDGHVLTVAIPQPNAAAVDA
                     ISQATGLAVYPVFSNPTDLEATRRRLSAG"
     gene            complement(2069..2803)
                     /locus_tag="VFV20_00785"
     CDS             complement(2069..2803)
                     /locus_tag="VFV20_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006547601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5286914.1"
                     /translation="MKLLVVDDDRDLVELLDYALRREGYDVVRAYDGEAAVEAVGRER
                     PDLVVLDINLPKLTGFVVLERIRHVDDHVAILMLSARQDESDVLRGLQLGADDYITKP
                     FRPKELVARVKAILRRSVRTRGRPAPSVYRTGDLVLDEATHLVSAADVPVHLSPTEFK
                     LLRHFMANPGRVLTQEEILEGVWGYDADVGGDLVKLNVSRLRRKLTQDGKSRDVIQTV
                     PGVGYLFRGADGGVTREEARPKSGAS"
     gene            complement(2800..3879)
                     /locus_tag="VFV20_00790"
     CDS             complement(2800..3879)
                     /locus_tag="VFV20_00790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5286915.1"
                     /translation="MSAERRRSPGRRASDHAIAPIDVQDAFVALTDGILLTDPEGVIV
                     SANPAAFRVLGEDRLVGKPFDELVLVSGATRVQDTEGHRVRRAWFPRDERMGVLEIVS
                     TTLGDRGSLHTVRDVTAQAELLRLKEDFLLQVAHELRTPIAALSATLDLLVEDALSMS
                     RDELGSMVATLRRSALRLEHLVDNLLDAGSIEAGTFQVRAMPTSVRHSLQEALVFVQP
                     LLDAKGQHLVMDLRRDADRVLADARRTGQVLANLLGNASKYAPEGSTITVASETREGF
                     VRVAVTDQGPGIPEDEQARLFQRFFRSREVRDQAGGLGLGLSICRAIVRAQGGEIGIQ
                     SAPGRGTSVHFTLPKARHLTEDASQ"
     gene            complement(3886..>4535)
                     /locus_tag="VFV20_00795"
     CDS             complement(3886..>4535)
                     /locus_tag="VFV20_00795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5286916.1"
                     /translation="ARDLHDGVAQTIANAAIGIELARKRAKIDVDAALVELDVLRTRL
                     VESQKGLRDILFALRPLPLEQGGLAVAVRSLAERMDGTNGSVVRARKTESTRRLRAEI
                     EAGAFTVIREAANNALKTGRAPHVDLDVYDEPDAVVAIVEDDGQGFDVAAVLGSYSTR
                     GSLGLLQMRESARLIGAQLSIDSSPGNGTKIRLRVPVPDATREHRDEATDASARE"
BASE COUNT          638 a         1614 c         1582 g          701 t
ORIGIN      
        1 cgatctgcgc ggagcgcgat cgcggcggct cgttcgggtc tttcgacgaa ttcgcgcgtc
       61 gtgtggggct gaaggaggag gcgctgcgca atctcgcgct cgtgggggca ttcgatgcgt
      121 tcggcgagca gcgtcgcgcg ctgctctggc gcgcgcgcga cgcgcatcgc acgtcgccgg
      181 cgttcgcgcg gccgccgctc gcgctgccgg ccaccgacgc gccgcggctg ccgatcctgt
      241 cggagcgcga gcgcgtcgcg ctggactatc gcatcaccgg catcccgacc ggtccgcaga
      301 tcatgcggtt ctatcgcgac gagctcacgc ggcgcggcgt gctctccgcg atcgagctcg
      361 cggaccacgc gcacggcgag ctcgtgcgca tcgggggcgc gatcgtcgtg aagcagcatc
      421 cggagaccgc gaagggacac gtgttcctct cgctcgagga cgagaccggg atggcgaacg
      481 tcatcatccg tccggcgacg tatcggcggt tcaagcgtgt gctggacgcg tacgccgcgg
      541 tcgtcgtggc cggcgtgctg caaacggtgg acggcgtcat ctccgtgctg gccgcgcggc
      601 tggacgggct cgacctgttc gtggagatga aggcgcgcga gtggcattag tgcgcctaaa
      661 caccggcgtc aggtatcagg gcggtaggca aaccggtagc agggccttgt cccatcgcct
      721 cgctcctgcg caaactccac tgggcaacag cagggaggca ggcgtccgcc tggtcccgct
      781 cggttctcgc aggcgcagga gaggggatgg acatcgcgac cgatcgtcga gcactagatc
      841 tgctcgactc gatctttcgt gttgccacgg agctcgttcg tggggagcgc gcgtcgctgc
      901 tcttgcgcga cgacgagacg agcgacttcg tgatcgcgcg cgcacacggc ctcgccgagg
      961 aagtgcagcg gcaggtgcgc gtccggtcgg gtgtgggcat cgcgggacac gtgctggcct
     1021 cccggcagtc gctcctcgtg cgcgggccgc aggacgctcc gatcccgatc agcggcgacc
     1081 gctatcgctc gagctcgttc atctcggtgc cggtgatcgt cgacgacgag ccgcgcgccg
     1141 tcctcagcgt cgcggatcgc accgacggtc tgcccttcga gcaggcggac ctgcagacgc
     1201 tcgagatcct cgcgggccac atcggcgcgt gcctggtgca gcaggagcag ggcgaggccc
     1261 ttcagcgcct ggccgagacc gacccgctca cgtggctctt caaccgccgt cacttcgaca
     1321 agcgcctcga gtcggagacg aaccgcgcga tgcgagccga gaacctcctc gcgctcctca
     1381 tgatcgacgt ggacaagttc aagacgatca acgatcgctt cggccatcac gtgggcgacc
     1441 aggtgctcaa ggccgtcgcg gccgcgatca agcaggcggt ccgcctctac gacgtgccga
     1501 cgcggtacgg cggtgacgag ttcgcgatca tcctccccga gtcggacacg gacgtcgcct
     1561 cgcgcgtcgc gcgccgcatc ctcgagaaga cggaggccgc cgcgatcccc ggcgagctgc
     1621 gcgacgccgg tatgcacatc gagctctcca tcggcgtcgc cacgttcccg cgtccgctcg
     1681 gcgatgcgac cgcgctcgtc gaggcggcgg acaccgcgat gtaccgcgcg aaggaggccg
     1741 gcggcggcgt ccgcgtgtgg gagcactcgt tcgcggaggg accgcgcggc gcgctccgct
     1801 cgggccgtgt cgcgttgccg ccggcgccgt accttgccga tccggcgcgc ctcgcgacgc
     1861 gcgagctgca gcagctcgtg cccgccgcgc tcgcgcagga gtggaacgcc gtcgtcgtcg
     1921 gccgggacgg ccacgtgctg accgtcgcga tcccgcagcc caacgccgcg gcggtggatg
     1981 cgatctcgca ggcgacggga ctcgcggtct accccgtctt cagcaatcca acggacctcg
     2041 aagcgacgcg ccgccgcctc tccgccggct agctcgctcc gctcttcggc cgggcctcct
     2101 cgcgcgtgac gccgccgtcc gctccgcgga acagataacc cacaccgggg acggtctgga
     2161 tcacgtcgcg cgacttgcca tcctgcgtga gcttgcggcg cagccggctc acgttgagct
     2221 tcacgaggtc cccgccgacg tcggcgtcgt agccccacac accctcgagg atctcctcct
     2281 gcgtgaggac gcgaccgggg ttcgccatga agtggcggag cagcttgaac tcggtcggcg
     2341 agagatgcac cgggacgtcg gccgcgctga cgaggtgcgt ggcctcgtcg aggacgaggt
     2401 cgccggtccg gtacaccgac ggcgccggcc gtccgcgcgt ccgcaccgag cgccggagga
     2461 tcgccttcac gcgagcgacg agctccttcg gccggaacgg cttggtgatg taatcgtcgg
     2521 ccccgagctg cagcccgcgc aggacgtcgc tctcgtcctg gcgtgccgag agcatcagga
     2581 tcgcgacgtg gtcgtccacg tgccggatgc gttccagcac gacgaagccg gtgagcttgg
     2641 gcagattgat gtcgagcacg acgagatcgg ggcgctcgcg cccgacggcc tccaccgcgg
     2701 cctcgccgtc gtacgcgcgc acaacgtcgt agccttcccg tcgcagcgcg tagtcgagca
     2761 gctccaccag gtcgcggtcg tcgtcgacga ccagcagctt cactgcgagg cgtcctccgt
     2821 caagtgtctc gccttgggca gcgtgaagtg tacggatgtt ccacggcccg gcgcgctctg
     2881 gatcccgatc tcgccgccct gggctcgcac gatggcccgg cagatcgaaa gccccagacc
     2941 cagtccgccc gcctggtcac gcacctcgcg cgagcggaag aatcgctgga agagccgcgc
     3001 ctgttcgtcc tcggggatgc ccgggccctg gtcggtgacc gcaacgcgaa cgaagccctc
     3061 tcgggtctcc gacgcgacgg tgatggtgga cccctcgggg gcgtatttgc tcgcgtttcc
     3121 gaggagattc gcgagcacct gaccggtgcg gcgcgcgtcc gcgaggacgc gatcggcgtc
     3181 cctccgcagg tccatcacca ggtgctggcc cttcgcgtcg agcagcggct gcacgaacac
     3241 gagggcttcc tgcaggctgt ggcgcacgct tgtcggcatc gctcgcacct ggaacgtccc
     3301 cgcttcgatg cttcccgcgt cgagcaggtt gtcgacgagg tgctcgagcc gtagcgcgct
     3361 gcgccgcagc gtcgcgacca tcgagccaag ctcgtcacgc gacatcgaga gcgcatcctc
     3421 gacgaggagg tcgagcgtcg ccgacagcgc cgcgatcggc gtgcgcaact cgtgcgcgac
     3481 ctgcagcagg aagtcctcct tgaggcgcag cagctccgcc tgcgcggtca cgtcgcgcac
     3541 cgtgtgcagc gagccgcgat caccgagcgt cgtgctcacg atctcgagga cgcccatgcg
     3601 ctcgtcgcgt gggaaccagg cgcgccgcac gcgatggccc tcggtgtcct ggacgcgcgt
     3661 cgcgccggag acgagcacca actcgtcgaa gggcttcccg acgagccggt cctcgccgag
     3721 gacccggaac gccgccgggt tcgcgctcac gatgacgccc tcgggatcgg tcaggaggat
     3781 cccgtcggtg agcgcgacga acgcgtcctg gacgtcgatg ggcgcgatgg cgtgatcgct
     3841 cgcgcgccgc cccggtgacc gtctgcgctc cgcgctcatg ccgacctact ctcgcgcgct
     3901 cgcgtcggtg gcctcgtccc gatgctcgcg ggtcgcgtcc ggcaccggca cgcgcagccg
     3961 gatcttcgta ccgttccccg ggctcgagtc gatcgacagc tgtgcgccga tgaggcgcgc
     4021 cgactcacgc atctggagca agccgaggct gccgcgcgtc gagtagctgc ccagcaccgc
     4081 cgcgacgtcg aatccctgtc cgtcgtcctc gacgatggcg acgaccgcat ccggttcgtc
     4141 gtacacgtcg agatcgacgt gcggcgcgcg gccggtcttc agtgcgttgt tcgcggcctc
     4201 gcggatgacc gtgaacgcgc cggcctcgat ctcggcgcgg agccgtctcg tcgactcggt
     4261 cttccgtgcg cgcaccaccg agccgttcgt cccgtccata cgctccgcga gcgaccggac
     4321 ggcgaccgcg aggccgccct gctcgagcgg cagcgggcgg agcgcgaaga ggatgtcgcg
     4381 gagccccttc tgcgactcga ccaggcgtgt tcgcaggacg tcgagctcga caagcgccgc
     4441 gtcgacatcg atcttggcgc gcttccgcgc gagctcgatc ccgatcgcgg cgttggcgat
     4501 ggtctgggcg acgccgtcgt ggaggtcgcg cgcga
//
LOCUS       DASTZR010000036         9672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1020973, whole genome shotgun sequence.
ACCESSION   DASTZR010000036 DASTZR010000000
VERSION     DASTZR010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9672
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020973"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1219)
                     /locus_tag="VFV20_00800"
     CDS             complement(<1..1219)
                     /locus_tag="VFV20_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding dehydrogenase"
                     /protein_id="HEU5286917.1"
                     /translation="MDADVIIVGAGLAGLVAAAEVADAGKRVILLDQEPEASLGGQAF
                     WSFGGLFMVDTPEQRRLRVRDSHDIALRDWLAYAGFDRDDDMWPRRWVEAYVAFAAGE
                     MRPWLHAQGMRWFPLVQWAERRGSSVPRFHITWGTGPGMIEPFVRRVRAHADAGRVAL
                     RFRHRVTHLSITNGAVDGVSGDLLAASDAERGAPSARDATGAFSLRADAVIVTSGGIG
                     GDHGLVRRYWPARLGTPPRRMLSGVPDHVDGRMLEVARAAGARAVNLDRMWNYPEGVE
                     NYAPVWSRHGIRILAGPSALWLDAAGHRLPAPNFPGFDALGTLERIARSGHDHSWFVM
                     DSAVFGREMALSGSEQNPDLTGRSVRMVLQRALPRAVKPVERFGERGADFVFAPSARA
                     LAGRMNALAGATEI"
     gene            complement(1382..1723)
                     /locus_tag="VFV20_00805"
     CDS             complement(1382..1723)
                     /locus_tag="VFV20_00805"
                     /inference="COORDINATES: protein motif:HMM:NF025170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCYCGC motif-containing (lipo)protein"
                     /protein_id="HEU5286918.1"
                     /translation="MIWTRRALLRAGVGLAAVACTGATGTATPTSGDGRPNDPLAANA
                     DLGVWPDRITKSSKEIQEAYAYAVRGPRSLQYIPCYCGCGASGHKNNQDCYVQQFLTK
                     GWVKLDLHGYG"
     gene            1862..2278
                     /locus_tag="VFV20_00810"
     CDS             1862..2278
                     /locus_tag="VFV20_00810"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019032267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEU5286919.1"
                     /translation="MSQITVHHESVDRFRIGIRGHEVVVDQPAPASGDTGPTPTELFV
                     ASLASCAGFYARRFLARHGLEDGELVVACDFGWAADHSRVTAIALRIETPRGVPDELR
                     PALMRVLEHCTVHESIRSVPTVTYDIAPARVAAAIS"
     gene            2342..3403
                     /locus_tag="VFV20_00815"
     CDS             2342..3403
                     /locus_tag="VFV20_00815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5286920.1"
                     /translation="MDGIGDGREQISLRQLLDALPVPVIVYGAGGAVLLANQARTEFA
                     GNDVATLDAAIARTRPAREDGEPVARDDLPALRALRGETVRGERLRLHAADGHTEVLL
                     VNASPLRDASGAVVAAVVVFHDITDLSELERGRRELFGMANHDLRTPLTVILGFVQLA
                     RRLSVKDPARALRVLDDIERQSQRMIRLVQDLLDVARFETGQIPVHPQPGDLAESVRA
                     AVARQPDGERIGVLIGDGHFVTRFDADRVDQVLDNLLSNAIRHTAPGTRVEVRVSVED
                     DEIVVRVTDHGGGVAPDELPRLFRPFYQTPRSRSYGGTGLGLHISRRVAEAHGGRLWL
                     DETGPGGTTFAFALPRVAT"
     gene            complement(3400..3753)
                     /locus_tag="VFV20_00820"
     CDS             complement(3400..3753)
                     /locus_tag="VFV20_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU5286921.1"
                     /translation="MADRGRATSRSRWALLCVLALAAGAACTSTTRAGEPVPRGVQVY
                     RDLKCGGCHESHFGLPSEAPALDHIGSVAGNRRPGEDAATYIREAALKMPGVRGARLP
                     DADLDALVEYLLSRD"
     gene            complement(3756..4520)
                     /locus_tag="VFV20_00825"
     CDS             complement(3756..4520)
                     /locus_tag="VFV20_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286922.1"
                     /translation="MGSTKSELARCIYEQGDPLVRLARAIHADPELAVSAFDASARLV
                     EFLCDNGFEIRIGICGLATAFIASRGAGALRLTLCADYDSLDGGVGHGSGHHLVAGAT
                     VGAAVGVAPFADDLGLTVSVLGTPGAELPRLLAGGAFDGTHAVLMIVPTAAGLTPRVM
                     EYLPNRELRRDAPLRRAFARNAAALGLTAQIGTRDFAARSDTDDAYQEMLHAAVALAW
                     TTFDAATARELRAHLMETADTSAAARHRQRRERGGR"
     gene            complement(4558..5025)
                     /locus_tag="VFV20_00830"
     CDS             complement(4558..5025)
                     /locus_tag="VFV20_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU5286923.1"
                     /translation="MATLTSDFLSQKRIAVAGVSRKPASHGANAIYRRLKARGYEVFA
                     VNPKAGTVEGDQAYPDLRSIPGGVDGVVIATAARHADPIVREAKELGISRVWMHAGPA
                     ESSVSPTAVDYCRANGMSVIPGGCPLMFGGTADLGHRCMRWLLEQRGVVPRGI"
     gene            complement(5057..5338)
                     /locus_tag="VFV20_00835"
     CDS             complement(5057..5338)
                     /locus_tag="VFV20_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HEU5286924.1"
                     /translation="MTQLTDKIIATFNLDRENVDKVIAKIEALINEGNVRQAIVKDAN
                     GGTVLKAPLTLGIVGVALAPFWAAIGAIAALAADFTIQIEAGPDQGDQA"
     gene            complement(5374..5688)
                     /locus_tag="VFV20_00840"
     CDS             complement(5374..5688)
                     /locus_tag="VFV20_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286925.1"
                     /translation="MINAARWWPFAQPSSLPGKAVLLATTLLASQLHESTGSAQRALT
                     DGSYGVVAVGSVLGMAMGATVVRRLFGKNTAFIRSDLAAMVLVVVLVAVKIAIARIFL
                     PL"
     gene            5850..8105
                     /locus_tag="VFV20_00845"
     CDS             5850..8105
                     /locus_tag="VFV20_00845"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEU5286926.1"
                     /translation="MGTRGTRVAPAPGPLGSFVGRRQELTRLRTLLSETRLVTVAGPG
                     GAGKTRLAEELAAGMTRSFQGAIAFAYLAAAQTAAEVLDLTAAAVGIRGSGQQDLRQQ
                     LTDYCAAHPYLLVLDNCEHVAGPAAELAACLVASCPRVRILATSRRPLHVPGEQIFPL
                     GGLGTEAATLLFVDRARRAIPDLALDGTARSHVARICSRLESMPLAVELAAAQVRSIG
                     LAELAERVERDASGLSTRSILAPERQRTLRHTVAWSHDLMDEPQRIVWRRLGVFSGGF
                     TLAAAERVVALPPIDAAAVPDLLGDLVDQSMVVFDAATDRYRLIEVLREFAHERLEAS
                     GEEARVTEQHRRWLVDLVGECDRRWYGPDQPRILDVLEREGANLRAALERCRREHADL
                     DGLRLANGAFFYWLARASLVEGSRWYAEFLGRSGDPALEARSHWRAGYLATITMDLPL
                     AERLLARASAIIAEAGDPVDRAYARVILALALLWQHPEEHERARRLAQETATDPTADA
                     MARSWASIVLALRALALHDFAACRDLSLAAETPTRAAGDLWSLERILLFLAHGEWQLG
                     ELAAAEAHLIESVGIARTMDDLGHLAWAADALGWVTLDRGNPARAARLLGIADSAWIR
                     SGAGLAEPWRPWRDSAMTRLSEALGAKRLAAEMGAGRDLAHGAAFAYVLDESPPASPR
                     ESRSSDLSARELEVAALVADGLGNRAIAERLFLSPRTVEKHIEHLMNKIGVDSRAAVA
                     AWHARSGTTSH"
     gene            complement(8141..8608)
                     /locus_tag="VFV20_00850"
     CDS             complement(8141..8608)
                     /locus_tag="VFV20_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEU5286927.1"
                     /translation="MAEKKKARSAGASAKSSSGARSGFTAEERAAMRERAQELKAAAS
                     KAEGEKAVRAAIAAMPPSDRAMAERLHAIIKESAPELSPKTWYGMPAYAKDDKVVVFF
                     KNASKFKERYATLGFDTAANLDDGEMWPTSFALTKLTRADEAKIAALVKKAVS"
     gene            complement(8611..9042)
                     /locus_tag="VFV20_00855"
     CDS             complement(8611..9042)
                     /locus_tag="VFV20_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5286928.1"
                     /translation="MITKLNVTSVKVLDQEEALDFYVNKLGLEKGNDVKQGPFRWLTV
                     RVPGDSGTEIFLEEPGPPLHDDATAAQLRELITKGAMSSLVFNSDDVFGLYETLQARG
                     VTDFTQEPIEHAYGTDMGIRDPFGNAIRILQQGKPARKVTA"
     gene            complement(9087..9536)
                     /locus_tag="VFV20_00860"
     CDS             complement(9087..9536)
                     /locus_tag="VFV20_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020515947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5286929.1"
                     /translation="MSRDVEDLNRRLLRARDAMDRSYAEPLDVRALAAMAHVSEAYFS
                     RSFRATFGETPHRYLQRRRVERSMFLLRETERSITDICVDVGFSSLGTFSRTFREIVG
                     ETPSEYRLGHEPMPAPNCVQMAGTRPVAASGPARSGSFGEANAPTPP"
BASE COUNT         1378 a         3433 c         3402 g         1459 t
ORIGIN      
        1 cgatctccgt cgcgccggcg agcgcgttca tcctgcccgc cagcgcgcgc gcgctgggcg
       61 cgaacacgaa gtcggcgccg cgctctccga accgctccac cggcttcacc gcgcgcggca
      121 acgcgcgctg gagcaccatc cggacgctcc ggccggtgag gtcggggttc tgctccgacc
      181 ccgagagcgc catctcgcgc ccgaagacgg cggaatccat gacgaaccac gagtggtcgt
      241 ggccggaccg cgcgatgcgc tcaagcgtgc ccagcgcgtc gaagcccggg aagttcggcg
      301 cgggaaggcg gtgtccggcc gcgtcgagcc acagcgccga cggccccgcg aggatgcgga
      361 tgccgtggcg cgaccatacg ggcgcgtaat tctcgacgcc ctccgggtag ttccacatgc
      421 ggtcgaggtt gaccgcgcgg gcaccggccg cgcgtgcgac ctcgagcatc cgtccatcga
      481 cgtgatcggg aacgccggac agcatccgcc gcggtggggt gcccagccgc gccggccagt
      541 accggcgcac gagcccgtga tcgccgccta tcccacctga cgtgacgatc acggcgtccg
      601 cgcggaggct gaacgcgccg gtcgcgtctc gcgcgctcgg cgcgccgcgc tcggcgtcgg
      661 acgcggcgag aagatccccg gacacgccgt cgaccgcgcc gttcgtgatc gagaggtgcg
      721 tcacgcggtg acggaagcgc agcgccacgc gaccggcgtc ggcgtgcgcg cgcacgcggc
      781 gcacgaacgg ctcgatcatg ccggggccgg tgccccaggt gatgtggaag cgcggcacgc
      841 tcgatccacg acgctcggcc cactgcacga gcgggaacca gcgcatcccc tgcgcgtgga
      901 gccacggccg catctcgccc gcggcgaagg ccacgtacgc ctcgacccag cgtcgcggcc
      961 acatgtcgtc gtcgcgatcg aagccggcgt acgcgagcca gtcgcgcagc gcgatgtcgt
     1021 gcgagtcgcg gacccggagg cggcgctgct ctggggtgtc gaccatgaag aggccgccga
     1081 aggaccagaa cgcctgaccg ccgagcgatg cttccggctc ctggtcgagg aggatgacgc
     1141 gcttgccggc gtccgcgact tccgcggcgg cgacgagtcc ggcgaggccc gcgcccacga
     1201 tgatgacgtc cgcgtccatc gcgcggactc tagggcagcc tccctatgcc ggcggcagcg
     1261 gcgtgttcgt gccgggcccg cccttgcccc acttggcatc gatgagacgg cggatctccg
     1321 ggagcggctg gccctgtcgc tcgaatgcca tcacgtcacg cgtgataccg acgcaaacgc
     1381 ctcagccata cccgtgcagg tcgagcttca cccagccctt ggtgaggaac tgctgcacgt
     1441 agcagtcctg gttgttcttg tggcccgacg caccgcagcc gcagtagcac gggatgtact
     1501 gaagcgagcg cggtccgcgc acggcgtacg cgtacgcctc ctggatctcc ttcgacgact
     1561 tcgtgatgcg gtccggccag acgcccaggt ccgcgttcgc cgcgagggga tcgttcgggc
     1621 gtccgtcgcc ggaggtcggt gtcgcggtcc cggtcgcccc ggtgcacgcg accgcggcga
     1681 gcccgacacc ggcgcgcagc agggcgcggc gggtccagat catgtggggc ggacgatgac
     1741 gtggatgagg acgacacgct agggatgaat ggcctctttc ggggaccttt ggccctcgct
     1801 ctcaggatgc gtcgcgccgc atcgtcgaga tgcgccggcg cgtccggcgg gaggtcagcg
     1861 catgagccag atcaccgtcc accacgagag cgtcgaccgc ttccgcatcg gcatacgcgg
     1921 acacgaggtc gtcgtcgatc agccggcacc ggccagcggc gacaccggtc cgacgcccac
     1981 cgagctcttc gtcgcgagcc tcgcgtcctg cgccggcttc tatgcgcggc gcttcctggc
     2041 gcgccacggc ctcgaggatg gcgagctcgt ggtcgcgtgc gacttcggct gggcggcgga
     2101 tcactcgcgc gtgacggcga tcgcactccg gatcgagacg cctcgcggcg tgcccgacga
     2161 gctccgcccg gcgctcatgc gcgtgctcga gcattgcacc gtgcatgagt cgatccggtc
     2221 cgtgccgacc gtgacgtacg acatcgcgcc cgctcgcgtc gccgccgcca tcagctgacc
     2281 cgctggagcc tcgcggccac atttcgcgct cggcgcgcgg aggctggtcg atactcctcc
     2341 ggtggacggc atcggcgacg ggcgcgagca gatctcgctc cggcagctgc tggatgcgct
     2401 gcccgtcccc gtcatcgtgt acggcgccgg cggtgcggtc ctcctcgcca atcaggcgcg
     2461 cacggagttc gccgggaacg acgtggccac gctcgacgcg gcgatcgcgc gcacgcggcc
     2521 cgcgcgagag gatggcgagc cggtcgcgcg tgacgacctt ccggcgctcc gcgccttgcg
     2581 cggggagacg gtccgcggcg aacgtctccg cctgcacgcc gccgatggtc acaccgaggt
     2641 gctcctcgtg aacgcctcgc cgctccgcga cgcgagcggc gcggtggtcg cggcggtcgt
     2701 cgtgttccac gacatcaccg acctcagcga gctcgagcgc gggcgccgcg agctgttcgg
     2761 gatggcgaac cacgatctgc gcacaccgct caccgtcatc ctcggcttcg tccagctcgc
     2821 ccggcgcctg tcggtgaagg atcccgcacg cgctctacgg gtcctcgatg acatcgagcg
     2881 tcagtcgcag cgcatgatcc gcctcgtcca ggacctgctg gacgtcgcgc gcttcgagac
     2941 cggtcagatc ccggtgcacc cgcagcccgg cgacctggcc gagagcgtgc gcgcagccgt
     3001 cgcgcgccag ccggacggcg agcggatcgg tgtcctcatc ggcgacggcc atttcgtgac
     3061 gcgcttcgac gcggaccgcg tcgaccaggt cctcgacaat ctgctctcga acgcgatccg
     3121 gcacaccgcg cctggaacgc gcgtcgaggt gcgcgtcagc gtggaggacg acgagatcgt
     3181 cgtgcgggtg accgaccacg gcggcggcgt ggcgccggac gagctgccgc ggctcttccg
     3241 tcccttctat cagacgccgc gcagccgcag ctacggtggc accggtcttg gactgcacat
     3301 cagccgacgc gtcgccgagg cgcacggcgg ccgtctctgg ctggacgaga ccggaccggg
     3361 aggcacgacg ttcgccttcg cgctgccgcg cgtcgctacc tagtcgcgag acagcaggta
     3421 ctcgaccagc gcgtcgagat ccgcgtcggg caggcgtgcg ccacgaacgc cgggcatctt
     3481 cagcgccgcc tcccgtatgt aggtcgccgc atcctcacct ggccggcggt tgccggcgac
     3541 ggagccgatg tgatcgagcg ccggcgcttc ggacggcagg ccgaagtgcg attcgtgaca
     3601 tccgccgcac ttcaggtcgc ggtagacctg gacaccgcgc gggacgggct cgccggcgcg
     3661 cgtcgtgctg gtgcacgctg ctccggcagc gagcgccagc acgcagagca gcgcccaccg
     3721 cgagcgcgac gttgcgcgac cgcggtcggc catgctcacc gtcctcctcg ttcacgtcgc
     3781 tgacggtggc gcgcggccgc cgacgtatcg gcggtctcca tgaggtgtgc acgcagctcc
     3841 cgcgccgtgg ccgcgtcgaa cgtggtccag gcaagcgcga ccgcggcgtg cagcatctcc
     3901 tggtacgcgt cgtccgtgtc cgaccgggcg gcgaagtcgc gggtgccgat ctgcgccgtg
     3961 agtccgagcg ccgcggcgtt cctcgcgaac gcgcgacgca gcggagcatc ccgccgcaac
     4021 tcgcggttcg gcaggtactc catgacgcgc ggcgtcaggc ccgcggcggt cgggacgatc
     4081 atgaggaccg cgtgtgtgcc gtcgaacgca ccgccggcga gcagcctggg cagctcggca
     4141 ccgggggtac cgagcaccga caccgtgagg ccgaggtcgt cggcgaacgg agcgacgccg
     4201 accgccgcac cgaccgtggc acccgccacg aggtgatgac ccgacccgtg gcccacgccg
     4261 ccgtcgagcg agtcgtagtc agcgcacaag gtcaggcgaa gcgcaccggc gcctcgcgac
     4321 gcgatgaatg cggtcgcgag gccgcatatc ccgatacgga tctcgaaccc gttgtcgcag
     4381 aggaactcga caagacgcgc gctcgcgtcg aacgccgaga cggcgagctc gggatcggca
     4441 tggatagcac gcgcgagccg tacgagcggg tcgccctgct cgtagatgca tcgcgcgagc
     4501 tcgctcttcg tgctacccaa tgccgttgcc ctcgtctttc atcggatcgc gccgtgctca
     4561 gatacccctc ggcacgaccc cgcgctgctc gagcagccag cgcatgcagc ggtgaccgag
     4621 atccgcggtg ccgccgaaca tgagcggaca gccgccgggg atgacgctca tgccgttcgc
     4681 gcggcagtaa tcgaccgccg tgggactgac gctgctctcg gcgggtcccg cgtgcatcca
     4741 cacgcgcgag atcccgagct ccttggcttc gcgcacgatc ggatccgcgt gcctcgccgc
     4801 cgtcgcgatc acgacgccat ccaccccacc ggggatggag cgcagatccg ggtaggcctg
     4861 gtcgccttcg acggtgccgg ccttcgggtt cacagcgaag acctcgtagc cgcgcgcctt
     4921 caggcgacgg tagatcgcgt tcgcgccgtg gctcgccggc ttgcgcgaga cgcctgcgac
     4981 cgcgatgcgc ttctgggaga ggaagtcgct ggtcagcgtt gccatgtcgt cgttcctccg
     5041 atgaaatctc gtgtggctat gcctgatcgc cctgatccgg gccggcctcg atctggatcg
     5101 tgaagtccgc ggcgagcgcg gcgatcgcgc cgatcgcggc ccagaacgga gccagcgcca
     5161 cgccgacgat gccgagcgtg agcggtgcct tgagaacggt gccgccattc gcgtccttca
     5221 cgatcgcctg gcggacgttg ccctcgttga tgagcgcttc gatcttcgcg atgaccttgt
     5281 cgacgttctc gcgatcgagg ttgaacgtcg cgatgatctt gtcggtgagc tgcgtcatgt
     5341 gactcctcca agacgaaatt cgacggatgg gagctagagc ggcaggaaga tgcgcgcgat
     5401 cgcgatcttg accgcgacca ggacgaccac caggaccatc gccgcgaggt cgctcctgat
     5461 gaacgcggtg ttcttgccga agagccgacg cacgacggtg gcgcccatcg ccatgcccag
     5521 aacgctgccg accgccacga ccccgtagct gccgtcggtg agggcacgct gtgccgagcc
     5581 ggtgctctca tggagctgcg acgcgagcag cgtggtcgcg agcaggacgg ccttgccggg
     5641 gagcgaggat ggctgcgcga atggccacca gcgggctgcg ttgatcatct ttgtcctcct
     5701 tgactcagtc cgcggtcccg accgcggctg tggcggacga tgccgcgcgc cgacggaccg
     5761 aacatcggga gatcacccca tcgggtacgt atccggccac gtattccggc tttggaggcc
     5821 gagtaggatg cggcccggag ggaagatcgg tgggcacgcg cgggactcgt gtcgcccctg
     5881 cgcctggacc gctcggatcg ttcgtcggcc ggcgtcagga gctgacgcgc ctgcggacgc
     5941 tgctgtccga aacccgtctc gtgaccgtcg ccgggcccgg cggcgcagga aagacccggc
     6001 tcgccgagga gctggcggcg gggatgacga ggtcattcca gggagcgatc gcgttcgcct
     6061 atctggccgc ggcgcagacg gcggccgagg tgctggacct caccgccgcc gccgtcggca
     6121 tccgtggaag cggtcagcag gacctacggc aacagctcac ggactactgc gccgcccacc
     6181 cctacctgct cgtcctcgac aactgcgaac acgtcgcggg acccgccgcg gagctcgcgg
     6241 cgtgtctggt cgcatcgtgt ccgcgcgttc gcatcctcgc gacgagcagg cgtccactcc
     6301 acgtgccggg cgagcagatc ttcccgctgg gcggactcgg gaccgaggcc gcgacgctgc
     6361 tgttcgtcga ccgcgcgcgt cgcgcgatac ccgatctcgc gctcgatggc acggcgcgat
     6421 cgcacgtggc gcggatctgt tcgcgtctcg agtcgatgcc cctcgccgtc gagctcgcgg
     6481 cggcgcaggt gcggagcatc ggcctggcgg agctggccga gcgcgtcgag cgcgacgcgt
     6541 cggggctgtc gacccgaagc atcctcgcgc ccgagcgcca gcggacgcta cggcacaccg
     6601 tcgcgtggag tcacgacctg atggacgagc cccagcgcat cgtgtggcgg cgcctcggcg
     6661 tcttctccgg cggcttcacc ctggccgccg cggaacgcgt cgtcgcgctc ccaccgatcg
     6721 acgcggcagc cgttcccgat ctgctcgggg acctcgtcga ccagtccatg gtcgtcttcg
     6781 atgccgccac cgaccgctac cgcctgatcg aggtgctgcg cgaattcgca cacgagcggc
     6841 tcgaggcgtc cggcgaggag gcgcgcgtca cggagcagca ccgtcgctgg ctcgttgatc
     6901 tcgtcggcga gtgcgacaga cggtggtacg ggcccgacca gccacggatc ctcgacgtgc
     6961 tcgagcgcga aggagcgaac ctccgcgcgg cgctcgagcg gtgccgccgc gagcacgcgg
     7021 atctcgacgg gctgcgtctt gcgaacggcg cgttcttcta ctggctcgcg cgcgcgagcc
     7081 tcgtcgaggg gtcgcgctgg tacgcggagt tcctcggacg ctccggcgat cccgcgctcg
     7141 aggcgcggtc acactggcgc gcgggatacc tcgcgacgat cacgatggat ctcccgctcg
     7201 cggagcggct gctcgcgcgc gcgtcggcca tcatcgctga ggccggcgac cccgtggacc
     7261 gagcgtacgc acgcgtcatc ctcgccctcg cgctgctctg gcagcatccc gaggagcacg
     7321 agagggcgcg gcgcctcgcc caggagacgg cgacggatcc gaccgctgat gcgatggcgc
     7381 gctcgtgggc aagcatcgtc ctcgcgctca gggcgctcgc gctccacgac ttcgcggcgt
     7441 gccgcgatct gtcgctcgcg gccgagacac cgacgcgtgc cgccggagac ctgtggagcc
     7501 tcgagcggat cctgctcttc ctcgcgcacg gcgagtggca gctcggagag ctcgcggccg
     7561 ccgaagccca cctgatcgag agcgtcggga tcgcgcggac gatggacgac ctcggacacc
     7621 tggcgtgggc cgccgacgcg ctcggatggg tgacgctcga tcgcggcaac ccggcgcgtg
     7681 ccgcgcgatt gctcggcatc gccgacagcg cgtggatccg gagcggcgca gggctcgcgg
     7741 agccgtggcg gccgtggcgc gactcggcaa tgacgcgcct atccgaggcg ctcggggcga
     7801 agcggctcgc ggcggagatg ggcgccgggc gggatctcgc gcacggcgcg gcgttcgcat
     7861 acgtcctcga cgaatctccg cccgcgtcac cgcgggagtc gcgatcctcg gacctgtcgg
     7921 cgcgcgagct cgaggtcgcg gcactcgtcg ccgatgggct gggcaatcgc gccatcgccg
     7981 agcggctctt cctctcgccg cgcaccgtcg agaagcacat cgagcatctg atgaacaaga
     8041 tcggcgtgga ctcgagggcg gccgtcgccg catggcacgc gaggtcgggc acgacgagtc
     8101 actgacctcc gcgcgtcttg cgggccgcga gcaccgaccc tcagctcacc gccttcttca
     8161 cgagcgcggc gatcttcgcc tcatccgcgc gggtcagctt ggtcagggcg aaggacgtcg
     8221 gccacatctc tccgtcgtcg aggttcgccg cggtgtcgaa gccgagcgtc gcgtatcgct
     8281 ccttgaactt ggaggcgttc ttgaagaaga ccacgacctt gtcgtccttc gcgtacgccg
     8341 gcatcccgta ccaggtcttg ggggacagct cgggcgcgct ctccttgatg atggcgtgga
     8401 gccgctcggc catcgcacgg tcgctcgggg gcatcgccgc gatcgcggcg cgcacggcct
     8461 tctccccctc ggccttgctc gcggcggcct tcagctcttg cgcccgctcc cgcatcgcgg
     8521 cccgctcctc ggccgtgaat cccgaccgcg cgccgctcga gctcttggcc gaggcccccg
     8581 cgcttcgcgc cttcttcttc tccgccatcg tcaggccgtg accttccggg cgggcttgcc
     8641 ctgctggagg atccggatgg cgttgccgaa gggatcgcgg atgcccatgt cggtgccgta
     8701 ggcgtgttcg atcggctcct gggtgaagtc cgtgacgccg cgcgcctgga gtgtctcgta
     8761 cagaccgaag acgtcgtccg agttgaagac gagggagctc atggcgccct tggtgatgag
     8821 ctcgcggagc tgcgccgcgg tggcatcgtc gtgcagcggc gggcccggct cttcgaggaa
     8881 gatctcggtg ccgctgtctc cggggacgcg caccgtcagc cagcggaagg gaccctgctt
     8941 gacgtcgttt cccttctcga ggccgagctt gttgacgtag aagtcgagcg cctcttcctg
     9001 gtcgaggacc ttgacggatg tgacgttcag cttggtgatc atcgtttgac ctctcctctg
     9061 cgagcgcgat gtgaacgagg ctgacgctag ggcggagtcg gcgcgttcgc ttctccaaaa
     9121 ctgcccgatc gcgccggccc cgacgcggcg accggccgcg tgccggccat ctgcacgcag
     9181 ttcggcgccg gcatcggctc gtgccccagg cggtactccg acggcgtctc gcccactatc
     9241 tcgcggaacg tgcggctgaa ggtcccgagg ctgctgaagc cgacgtcgac gcagatgtcg
     9301 gtgatgctgc gctcggtttc gcgaagcagg aacatcgagc gctcgacccg ccggcgctgc
     9361 agataacggt gcggcgtctc gccgaaggtc gcgcggaagc tgcggctgaa gtacgcctcg
     9421 gagacatggg ccatggcggc aagcgctcgg acgtcaagcg gctcggcgta ggagcggtcc
     9481 atggcatcgc gcgcgcgcag caggcgacgg ttgaggtcct cgacgtcccg gctcatacgt
     9541 gaacgctacg ccatcaaaca gacgggcgcc gcctccggcc agcgccgact gttcattcga
     9601 ctacagaggg gaacgacgcc gcgaccgtgg cgtcgagatg gacagagcgc gaagggacgt
     9661 gcgctcacga gg
//
LOCUS       DASTZR010000037         2208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_237618, whole genome shotgun sequence.
ACCESSION   DASTZR010000037 DASTZR010000000
VERSION     DASTZR010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237618"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="VFV20_00865"
     CDS             <1..1047
                     /locus_tag="VFV20_00865"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5286930.1"
                     /translation="QVYMRDSGQIVPMQAFIDRDKFETKDFEPAVINYYKYQDKLQSM
                     PFNSSSSMLYYNKDAFKEVGLDPNKPPVTFTEIADAAKKLTKKDASGNVTRYGFGPSI
                     YGWLYEQLMATSGALYADNGNGRDNRATKVLWNSDASKTILNWWKAGVTGGYFYNPGI
                     DNDGAANSFNAQKTAMYIESTARLRGHINTVAGKFQIGTGLYPRPDNKPKDGGNIIGG
                     ASLYIMKSRPAEEQQAAWEFVKYAMTPPVQAQWQSDTGYYPIVKPAYDQAPSKEWATK
                     YPQFLTAINQIRDSPQNRFTNGAILGVMPQARQRTQKMIEAVLLGQATVDAAVDAAVS
                     DINAQIDKYNKANP"
     gene            1082..>2208
                     /locus_tag="VFV20_00870"
     CDS             1082..>2208
                     /locus_tag="VFV20_00870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEU5286931.1"
                     /translation="MQGDAAVATTREETVRSLVDRTVDLLIIGGGIIGAGIARDAALR
                     GLSVALVEQTDFASGTTSRPTRLIHGGLRYLELFDFGLVRSDMRERETLLGIAPHLVS
                     PLPFLLPMYHPSLWYRFKLRVGMFLYDLLSLDKSLPRRKWLSREAVLEAEPRLRRDGL
                     AGAWRFYDAQVPLVERLVVENVVDAEEHGALVLNHARAVDYLRDGDRITGARVHDEVG
                     GRALDIHARMTVNATGPWLDRTIASVRRTRRPLLRLTKGIHLVTPHATERAHVLFARS
                     DGRLFFVVPFLDSTIVGTTDTDYTGDPAEAAATNDDVRYLQDAAREAFPDAPFDTIHF
                     TWAGVRALVREEGVSEGEVSRKHALFDHERREGVAGLLSVVG"
BASE COUNT          412 a          769 c          702 g          325 t
ORIGIN      
        1 caggtctaca tgcgcgacag cggccagatc gtgccgatgc aggcgttcat cgaccgcgac
       61 aagttcgaga cgaaggactt cgagcccgcg gtcatcaact actacaagta ccaggacaag
      121 ctgcagagca tgccgttcaa ctcgtcgtcg tccatgctct attacaacaa ggacgcattc
      181 aaagaggtcg ggctggaccc gaacaagcca ccggtgacct tcaccgagat cgccgacgcg
      241 gcgaagaagc tcaccaagaa ggacgcgagc ggcaacgtca cgcgttacgg cttcggcccg
      301 tcgatctacg gctggctcta cgagcagctc atggcgacgt ccggcgccct ctacgccgac
      361 aacggcaacg ggcgtgacaa ccgtgcgacg aaggtgctgt ggaacagcga cgcatccaag
      421 acgatcctga actggtggaa ggccggcgtg accggcggct acttctacaa ccccggcatc
      481 gacaacgacg gcgcggccaa ctcgttcaac gcgcagaaga cggccatgta catcgagagc
      541 accgcccggc tgcgtggcca catcaatacc gtggcgggta agttccagat cggcacaggt
      601 ctctatccgc gccccgacaa caagcccaag gacggcggga acatcatcgg tggtgcgtcg
      661 ctctacatca tgaagagccg ccccgccgag gagcagcagg ccgcgtggga gttcgtgaag
      721 tacgcgatga cgcccccggt gcaggcgcag tggcagtccg acacgggcta ctacccgatc
      781 gtgaagccgg cctacgacca ggctcccagc aaggagtggg cgaccaagta tccgcagttc
      841 ctcaccgcga tcaaccagat ccgtgactcg ccgcagaacc gcttcaccaa tggcgcgatc
      901 ctcggcgtca tgccgcaggc acgccagcgg acgcagaaga tgatcgaagc ggtcctgctc
      961 ggccaggcga ccgtcgacgc cgcggtcgat gccgcggtga gcgacatcaa cgcgcagatc
     1021 gacaagtaca acaaggccaa tccatagcgg ggcagccctg ctgccccgct ctgctggcct
     1081 gatgcagggc gacgccgcag tagccacgac acgagaggag acggtgcggt cgctcgtcga
     1141 ccgcaccgtc gatctcctga tcatcggcgg cggcatcatc ggagcgggca tcgcgaggga
     1201 tgcggcactg cgcggactgt ccgtcgccct cgtggagcag accgacttcg cgtctggcac
     1261 cacgtcacgg cccacgcggc tgatccacgg cgggctccgt tacctcgagc tgttcgactt
     1321 cgggctggtg cgaagcgaca tgcgcgagcg cgagacgctg ctcggcatcg cgccccatct
     1381 ggtgtcgccg ttgcccttcc tgctgccgat gtatcacccg agcctctggt atcgcttcaa
     1441 gctccgcgtc ggcatgttcc tctacgacct gctctcgctc gacaagagcc tgccgcgccg
     1501 caagtggctc agccgcgagg cggtgctgga ggcggagccg cgtctccgcc gcgacggcct
     1561 cgcgggcgca tggcgcttct acgacgccca ggtgccgctc gtcgagcgtc tcgtcgtcga
     1621 gaacgtcgtc gatgccgagg aacacggcgc gctcgtcctc aaccacgcgc gcgcggtgga
     1681 ctacctgcgc gacggcgacc ggatcaccgg cgcccgcgtg cacgacgagg tcggcggacg
     1741 ggccctggac atccacgcgc gcatgaccgt gaacgcgacc ggtccatggc tcgaccgcac
     1801 gatcgcatcc gtccgtcgca cgaggcggcc gctcctgcgg ctcacgaaag gcatccacct
     1861 cgtcacaccg cacgcgacgg agcgcgcgca cgtgctcttc gcgcggagcg acggacgcct
     1921 gttcttcgtg gtcccgttcc tcgactcgac catcgtcggc accaccgata ccgattacac
     1981 gggtgatccg gcggaggccg cagcgaccaa cgacgacgtt cgctacctcc aggatgccgc
     2041 acgcgaggcc ttcccagacg cgccgttcga cacgatccac ttcacctggg ccggcgtgcg
     2101 cgcgctcgtc cgcgaggaag gggtgagcga gggcgaggtc tcgcgcaagc acgcgctctt
     2161 cgaccacgaa cgccgcgagg gcgtggccgg tctgctctcg gtcgtcgg
//
LOCUS       DASTZR010000038         3133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1203250, whole genome shotgun sequence.
ACCESSION   DASTZR010000038 DASTZR010000000
VERSION     DASTZR010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203250"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VFV20_00875"
     CDS             <1..402
                     /locus_tag="VFV20_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286932.1"
                     /translation="PWLTITALGVVAMPAAIVLHNVLSALVGGEEAVSFIVALMVAPS
                     CIAVGVIGAAVVLLRERHELGVAVCVAAAGIGLFAAYMVFALVVTTIEGRNPAYQGTV
                     ELAVLPLGTLAAIGGALLSVGALMREGTAAA"
     gene            complement(416..1567)
                     /locus_tag="VFV20_00880"
     CDS             complement(416..1567)
                     /locus_tag="VFV20_00880"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019608574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU5286933.1"
                     /translation="MPKSTRGRPGFETLAIHAGQEPEGLTGAVTVPIYQTSTFAQEAV
                     GKNKGYEYARTGNPTRTALETCLAALEDAAWGLAFGSGMAATDAIAHLLSAGDHVVMG
                     DDVYGGTYRLFARAFDRLGISLTAVDMREPTNARKAMRKSTRLVWIETPTNPLLKVVD
                     IAAIAEIAHKGGALAVADNTFASPYLQNPLLHGVDLVLHSTTKYIGGHSDVVGGAIVG
                     RDEALREKLAFLQNAVGGVPGPLDAWLVLRGAKTLAVRMERHSANGQAIAEWLTEHPK
                     VKRVNYPGLAGHPQHDLARRQMKSFGGMLSFELRGGEAAARRAVARTKIFALAESLGG
                     VESLIEVPLAMTHGSVKGTKLAPPAGLVRLSVGIETVGDLIADLAQAIG"
     gene            1635..2006
                     /locus_tag="VFV20_00885"
     CDS             1635..2006
                     /locus_tag="VFV20_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5286934.1"
                     /translation="MGEERDRPRVLVFEDDGDVASLIVEILTSEGFDALHADHESAPD
                     ALVRQRPHLLLLDLCLGARPGGEVLSALRRSGLGDDVPVLLLSGKGDLEAQGHALGAA
                     ATLNKPFEIDALVATCWSLTA"
     gene            2150..2599
                     /locus_tag="VFV20_00890"
     CDS             2150..2599
                     /locus_tag="VFV20_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286935.1"
                     /translation="MQVVVELVAARVIGDELRFTIQTAACPRTGDPDATARAELATLF
                     PDFWLRRAIVHSTSWRYEDERIVLTYLGYSDEFDVAPLPLTIPLARASDIRRGPAAVA
                     AHAIRHLAFLVREARDGYGQLLRPDTHAALIAVAPDVSGRHDLKGAA"
     gene            complement(2603..>3133)
                     /locus_tag="VFV20_00895"
     CDS             complement(2603..>3133)
                     /locus_tag="VFV20_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5286936.1"
                     /translation="DASAVMVVLFADSGRSYLSKIYNDEWMRQNGYLERYPVHATVLD
                     VVKDRAGTPGLIVVSKGQTVRSAIDTMQRYGISQIPVVADGEATRVGDIVGSVQEKTM
                     LDRVFREPGLVEERVERVMEAPFPVVQANEDVERLYAELSAGAPALLAAADDRPVSII
                     TKADLLEFVAHQRRAR"
BASE COUNT          447 a         1120 c         1072 g          494 t
ORIGIN      
        1 ccctggctca ccatcacggc gctcggggtc gtcgcgatgc ccgcggccat cgtgctgcac
       61 aacgtcctga gcgcgctcgt cggcggcgag gaggcggtct ccttcatcgt ggccctcatg
      121 gtcgcgccgt cgtgcatcgc cgtcggcgtg atcggcgcgg ccgtcgtgct gctccgggag
      181 cgccacgagc tgggtgtcgc ggtctgcgtt gcggccgccg gcatcgggct cttcgcggcg
      241 tacatggtct tcgcgctggt cgtcaccacg atcgagggcc gcaacccggc gtatcaggga
      301 acggtcgagc tcgccgtcct tccgctgggg acgctcgcgg cgatcggagg ggcgctgctg
      361 tcggtgggcg ccctcatgcg cgaagggacc gccgccgcct agagccccgc gcgcctcagc
      421 cgatcgcctg cgcgaggtcg gcgatgagat cccccaccgt ctcgatcccc accgagagcc
      481 gcacgaggcc ggcgggcggc gcgagcttgg tgcccttcac cgagccgtgc gtcatcgcca
      541 ggggcacttc gatgagcgac tccacgccgc cgagcgactc cgccagcgcg aagatcttcg
      601 tgcgcgccac cgcgcgccgc gccgccgcct cgccgccgcg cagctcgaac gacagcatcc
      661 cgccgaacga cttcatctga cggcgcgcga ggtcgtgctg cggatggccc gcgagcccgg
      721 ggtagttgac ccgcttcacc ttcggatgct cggtgagcca ttccgcgatc gcctggccgt
      781 tggccgagtg ccgctccatg cgcaccgcga gcgtcttcgc cccgcgcagc acgagccacg
      841 cgtcgagggg accgggcacg ccgccgaccg cgttctgcag gaacgcgagc ttctcgcgca
      901 gcgcctcgtc gcgaccgacg atcgcgccgc cgaccacgtc ggagtgtcct ccgatgtatt
      961 tcgtcgtcga gtgaagcacc aggtcgacac cgtgcagcag cgggttctgc aggtacgggc
     1021 tcgcgaaggt gttgtccgcc accgcgagcg ccccaccctt gtgcgcgatc tcggcgattg
     1081 cggcgatgtc gaccaccttg agcagcgggt tcgtcggcgt ttcgatccag acgaggcgcg
     1141 tgctcttccg catcgccttc cgcgcgttcg tcggctcgcg catatcgacc gcggtgaggc
     1201 tgataccgag tcgatcgaag gcgcgcgcga agaggcggta ggtccccccg tagacgtcgt
     1261 cgcccatcac gacgtggtcg ccggcggaca ggagatgcgc gatggcgtcc gtcgcggcca
     1321 tgcccgatcc gaacgcgaga ccccacgcgg cgtcctcgag cgcggcaagg cacgtctcca
     1381 gcgccgtgcg cgtgggattg cccgtgcgcg cgtactcgta gcctttgttc ttcccgaccg
     1441 cctcctgcgc gaaggtcgat gtctggtaga tgggcacggt gaccgcaccg gtcaggccct
     1501 caggctcctg gcccgcgtgt atggcgagcg tctcgaaccc cggccggccg cgtgtggact
     1561 ttggcacgat gtgagtatcg ccgacaaaag gtcagtccat atccgttcgc gcgctcgtgc
     1621 gttagtaccg gcacatggga gaagagaggg accgtccgcg cgtcctcgtg ttcgaggacg
     1681 acggcgacgt cgcaagcctc atcgtcgaga tcctcacgag cgagggattc gacgcgctgc
     1741 acgctgatca cgagtcggct ccagacgccc tcgtacgcca gcggccacac ctcctgctgc
     1801 tggacctctg cctcggcgca cggccgggcg gcgaggtgct ttccgcactg cgtcgctcgg
     1861 gccttggcga cgacgtcccg gtcctcctcc tctcggggaa gggcgacctc gaggcgcagg
     1921 gccacgcgct cggtgctgcg gccacgctca acaagccgtt cgagatcgat gcgctcgtgg
     1981 cgacctgctg gagcctcacc gcctaagcgg ccagctctgg gcccgtcccg agaaccccag
     2041 cggacgtcca ggccctgaag gcaagccccc tcgtgcgact gaacggcacg gcctgtggca
     2101 tttgaaatgc gatggccggt accaagacac gagaaatggg agcacgcatg tgcaggtagt
     2161 cgtcgagctc gtcgcggcac gcgtgatcgg cgatgagctt cgcttcacga tccagacggc
     2221 ggcatgcccg cgcacgggcg atcccgacgc caccgcacgc gccgagctgg cgacgctctt
     2281 ccccgacttc tggctgcggc gggcgatcgt ccactcgacc agctggcgct acgaggacga
     2341 gcggatcgtc ctgacctacc tcggttacag cgacgagttc gacgtggctc cgctcccgct
     2401 gaccatcccg ctggcgcgcg cgagcgacat cagacggggc cccgcggcgg tcgcggcgca
     2461 cgccatccgc cacctggcct tcctggtgcg cgaagcgcgt gacggctacg gacagctgct
     2521 gcggccggac acgcatgcgg cgctcatcgc cgtcgcgccc gacgtgagcg gacgccacga
     2581 tctgaagggc gccgcgtagg cgtcagcgcg cgcggcgctg gtgggcgacg aactcgagca
     2641 ggtcggcctt ggtgatgatc gagacgggcc ggtcgtcggc cgcggcgagg agcgcgggcg
     2701 cgccggcgga gagctccgcg tagaggcgct ccacgtcctc gttcgcctgc acgaccggaa
     2761 agggcgcttc catgacgcgc tcgacccgct cctcgaccag acccggctcg cggaagacgc
     2821 ggtcgagcat cgtcttctcc tggacgctcc cgacgatgtc ccccacccgt gtcgcctcgc
     2881 cgtccgcgac gaccgggatc tgcgagatgc cgtagcgctg catggtgtcg atcgcggagc
     2941 gcacggtctg acccttggac acgacgatga ggccgggcgt gcccgcgcgg tccttgacca
     3001 cgtcgagcac ggtcgcgtgc acgggatagc gttcgaggta gccgttctgc ctcatccact
     3061 cgtcgttgta gatcttcgaa aggtacgagc ggccagagtc cgcgaagagc acgaccatga
     3121 ccgcgctcgc gtc
//
LOCUS       DASTZR010000039         2040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_759491, whole genome shotgun sequence.
ACCESSION   DASTZR010000039 DASTZR010000000
VERSION     DASTZR010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759491"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VFV20_00900"
     CDS             <1..432
                     /locus_tag="VFV20_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5286937.1"
                     /translation="GLDADDARIAAAVRAAALDADVAVLERGLDTLVGSRGVRLSGGQ
                     VQRAAAARALVRDAELLVVDDLSSALDVETERSLWDRLVPDGTAARTILAVSHRRPVL
                     RRADHIVVLKDGRVDAYGALDDLLRDSDEMRRLWRDDSAPA"
     gene            complement(433..1845)
                     /locus_tag="VFV20_00905"
     CDS             complement(433..1845)
                     /locus_tag="VFV20_00905"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU5286938.1"
                     /translation="MASLVDAPNDPILRGSELEPAGDPLRCTERAAPTSLKDVFEAAW
                     SPDSRTLAVSHIVTIPNHRTITGYEEDQRLALLDVATGKIVERGTGSEPAWSSSGTYL
                     SYWHDSDAVWVERRATGLPVAVLHPSQPNVRWAGDELFFFDGGEIHSWSTAGVQGVAT
                     VPAELAPRFPQDDVYFSADARWFTMTRYFQDGTTERYLGATKTGAMGALDDGGATYME
                     WSPVGDTLLLRSAATLTLRSASTGATLATVPTPPGTTHTWTADGRLLLGRIAPTVPGG
                     NAFDRLALLGDANAFATLPNLLGARTFSPDGRVFAGASRTGLYSTELELYRCGLAADE
                     GIDIANDPATKARAESIARDGRRFVRPATAAITQFVQGIHTGVDVAAPYGTLLVAADD
                     GVVDAVGWVPVGGRRVCVAHAGGLQSCDYHTALSLVTVGERVVRGQPVALMGMTGLTT
                     GPHVHWEAKRDGGIVDPLGE"
BASE COUNT          275 a          720 c          750 g          295 t
ORIGIN      
        1 ggactggacg cggacgacgc gcgcatcgcc gccgccgtgc gcgccgcggc tctcgacgcc
       61 gacgtggcgg tgctcgagcg cggactcgac acgctcgtcg gatcgcgcgg cgtgcggctc
      121 tcgggcggcc aggtccagcg cgccgccgcg gcgcgcgcgc tcgtgcgcga cgcggagctc
      181 ctcgtggtcg acgacctctc ctccgcgctc gacgtcgaga ccgagcggtc gctctgggac
      241 cggctcgtgc cggacgggac ggcagcgcgc acgatcctcg cggtctcaca tcgccgtccg
      301 gtcctccgtc gcgcggatca catcgtcgtg ctcaaggacg gacgggtcga cgcgtatgga
      361 gcgctcgatg acctgctgcg cgattccgat gagatgcggc ggctctggcg ggacgacagc
      421 gcgcccgcgt agctactcgc cgagcgggtc gacgatgccg ccgtcgcgct tcgcttccca
      481 gtgcacgtgc ggccccgtcg tcaggccggt catgcccatg agagcgaccg gctggccccg
      541 caccacgcgc tcgccgaccg tcacgaggct gagcgcggtg tggtagtcgc agctctggag
      601 tccgcccgcg tgcgcgacac agacccgccg tccgccgacc ggcacccacc cgaccgcatc
      661 gacgacgccg tcgtccgcgg cgacgagcag cgtgccgtac ggagccgcga cgtcgacgcc
      721 ggtgtggatg ccctggacga actgggtgat cgcggcggtc gcggggcgca cgaagcggcg
      781 cccatcacgc gcgatgctct ccgcgcgcgc cttcgtcgcc ggatcgttcg cgatgtcgat
      841 cccctcgtcg gccgcgagtc cgcagcgata gagctcgagc tccgtcgagt acaggccggt
      901 gcggctcgcg ccggcgaaga cgcgtccgtc gggactgaac gtgcgcgctc caaggaggtt
      961 cgggagcgtc gcgaacgcgt tcgcgtcgcc gaggagcgcg aggcgatcga acgcgttgcc
     1021 gccgggcacc gtcggagcga tgcggccgag gagcaggcgg ccgtcggcgg tccaggtgtg
     1081 cgtggtgccc ggcggcgtcg gcaccgtggc gagcgtggcg ccggtgctcg cgctccggag
     1141 cgtgagcgtc gcggcggagc gcaggagcag cgtgtcgccg accggcgacc actccatgta
     1201 tgtcgcgccg ccgtcgtcga gcgcgcccat cgcgcccgtc ttcgtcgccc cgaggtagcg
     1261 ctccgtcgtg ccgtcctgga agtaacgcgt catcgtgaac cagcgcgcat cggcgctgaa
     1321 gtagacgtcg tcctgaggga agcgcggcgc cagctccgcc ggcaccgtcg cgacgccctg
     1381 cacgcccgcg gtgctccagg agtggatctc gccgccatcg aagaagaaca gctcgtcgcc
     1441 ggcccagcgc acgttcggct ggctcgggtg caggaccgcc accggcagcc cggtcgcccg
     1501 gcgttcgacc cacacggcgt cgctgtcgtg ccagtacgag agatacgtgc cgctggacga
     1561 ccacgccggc tcgctgcccg ttccgcgttc gacgatcttg ccggtggcga cgtcaaggag
     1621 cgcgaggcgc tggtcctcct cgtaaccggt gatcgtgcgg tggttcggga tcgtgacgat
     1681 gtgcgagacc gcgagcgtgc gcgagtcggg cgaccatgcg gcctcgaaca cgtccttgag
     1741 gctcgttggc gccgcgcgct ccgtgcaccg gaggggatcg cccgcgggct cgagctcgct
     1801 cccgcggagg atcggatcgt tcggtgcgtc gacaagcgag gccacgctcg ctcgcgatac
     1861 gggcgcgcac gtgaccaacg tcacgacgag catcgcgagc cagatcgaac gcgtcacata
     1921 gtcggaacgg tcgtgtgtcg gaatcgcaac gcgaccggac gaacggcgtt catcggaacg
     1981 tgggatggcg atggggtcgt ctgctgatcg tgggaacgtt cgtgctgtcc gcgtgctcac
//
LOCUS       DASTZR010000040         5124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1256000, whole genome shotgun sequence.
ACCESSION   DASTZR010000040 DASTZR010000000
VERSION     DASTZR010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256000"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..603)
                     /locus_tag="VFV20_00910"
     CDS             complement(148..603)
                     /locus_tag="VFV20_00910"
                     /inference="COORDINATES: protein motif:HMM:NF020907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HEU5286939.1"
                     /translation="MDAAGGRIGAAFERAPLLVERLRGRVADGDDADAIMRTAWALIR
                     DMDEAQRIAILDAHPRIGAAPATLSALSRAEQGSDADTTVLRELETLNDEYERRFGFR
                     FVVFVHGRSKREIVSILRARLGRPRDVELAAALEEFLAISRDRLTRVSC"
     gene            complement(603..911)
                     /gene="uraH"
                     /locus_tag="VFV20_00915"
     CDS             complement(603..911)
                     /gene="uraH"
                     /locus_tag="VFV20_00915"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEU5286940.1"
                     /translation="MTRASISTHVLDTTAGRPAAGVHIELQQGGRAVAEGVTDGDGRV
                     RELAKDLEPGTYRLVFETQAYASDAFFQRVALDVELAPGHTHVPLLLSRYGVTSYRGS
                     "
     gene            935..1348
                     /locus_tag="VFV20_00920"
     CDS             935..1348
                     /locus_tag="VFV20_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HEU5286941.1"
                     /translation="MQMLRITAGKYTFRARLEEQLAPQTCAAIKKWLPIRSKLVHCRW
                     SGESTWVPMGEKRLGVEYENHTSYPAPGQVLVYPGGISEMEILFPYGSTRFASKVGQL
                     AGNHFATVVEGSEHLAEVGRLTLWEGAQDFTIELA"
     gene            1341..2714
                     /gene="allB"
                     /locus_tag="VFV20_00925"
     CDS             1341..2714
                     /gene="allB"
                     /locus_tag="VFV20_00925"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR03178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HEU5286942.1"
                     /translation="MRSLAVRGGRVVTSAGVAAADVLILEGTITGIVAPDAGQAREEI
                     DARGLVVLPGVVDAHVHVNEPGRTEWEGWHAATRGAAAGGVTTIADMPLNSVPPTLDG
                     ASFDAKYDAASNGAIVDFALWGGLVDADERRLRELAACGVVGVKAFTCPSGVDEFPHI
                     RDGALASALAAATRAGLLVAVHCEDEATVSVSTAHLRRAARRDARAWLESRPPEAERM
                     AIAQLAEAARATSAKVHVVHVSSHEAMRAVDRAQRIADLTAETCPHYLAFDAADLDEH
                     GALLKCAPPIRSGERDLLWRDILGPPPPFQRRVEYVASDHSPCSADLKTRGERDIFEA
                     WGGVSGLQSLLPVMLTEGVHGRGLALPELAQLVATRPAKRLGIWPRKGEIRGGTDADL
                     VLVDLDAEWTLDREALQTRSGLSPYVGRRFRGRIARTIVRGRTVYADGTITGAPGGGW
                     FVQRVNA"
     gene            2714..3952
                     /locus_tag="VFV20_00930"
     CDS             2714..3952
                     /locus_tag="VFV20_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEU5286943.1"
                     /translation="MATELVAIDERRLLRRLEELAQRGAQTSGGIYRALYTPAWVAAT
                     ELVSDWLKDAGLLVRRDAVGNVWGRIEGARGGRAIVTGSHIDTVRGGGALDGALGVVA
                     GIEAVASLVARYGRPARILECVAICEEEGSRFNTNFWGARAIADRLEPDEAARVRDAD
                     GTSLADAMRGVGLDPSRAAAAARRDIDTFVELHIEQGPILDDAAERVAVVRTIAGTAH
                     LEVVVRGEADHAGAAAMDRRRDALLGAAAMMLRIAEVAREMGPPAVATVGTIGVEPAQ
                     VNVVPGLARFTVDARHSDDALRRDLVADIEKACRVIGHERDLQVEVRTLRDRPPVTLN
                     ERTADVLRRACRAARVEPRDMTSGAGHDSQILVAAAHTGMLFVPSIGGRSHRADEATA
                     AADVVLGTRILATALHELAY"
     gene            3984..>5124
                     /locus_tag="VFV20_00935"
     CDS             3984..>5124
                     /locus_tag="VFV20_00935"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5286944.1"
                     /translation="MTDGGRAVPGDLVVSGSRIAALGPNAPAPSDAEVLDRPGHYVMP
                     GLVQPHVHLVQTLFRGLAEDLTLLDWLQRRVWPLETAHDEASVRAGVRLGLVELLTSG
                     TTTILDMGTTKHGDAVAEELVRSGIRASFGQAMMDTGEGVPAGLVESTRASLDASAAL
                     HKRWHRSANGRIRYAYAPRFALSCSRDLLEAVAALAKMNDLLVHTHSNEDPGERAAVE
                     RVTGRAPIAYLAETGIASERTVAAHGVHVDDAELAILAETRTAIAHCPTSNLKLAAGV
                     ADVARLRGARVTVGLGTDGAACNNRLDGFEEARLASLLARTLHGQGTLSAGDALVMAT
                     REGAKALHLDAEIGTLEVGKRADLVIIDPSRLGGPGGDPATRIVFG"
BASE COUNT          706 a         1843 c         1883 g          692 t
ORIGIN      
        1 cacgccggcg cagatgcggg cgcaggcaca gcgcatgtat cagatggccg tcgtctcgaa
       61 gctcatgccg ctcaacaacg tgaccggcat gacgccggcg gaacgcgagc tgctcgcgcg
      121 ctgggtgcgc tccggagcgc cgatccccta acaggacacg cgcgtcagcc gatcgcggga
      181 gatcgcgagg aactcctcga gcgcggccgc gagctccacg tcgcgcgggc gccccaggcg
      241 cgcacgcagg atcgagacga tctcgcgctt gctgcgcccg tgcacgaaca cgacgaaccg
      301 gaacccgaac cgccgctcgt actcgtcgtt cagcgtctcg agctcgcgga gcacggtcgt
      361 gtcggcgtcg gatccctgtt cggcacgcga cagcgcggag agcgtcgccg gagccgcccc
      421 gatgcgcggg tgcgcgtcga ggatcgcgat gcgctgcgcc tcgtccatat cacggatgag
      481 cgcccatgcc gtacgcatga tcgcgtcggc gtcgtcgccg tccgccacgc gcccccgcag
      541 ccgctcgacg aggaggggcg cgcgttcgaa ggccgcgccg atgcggccgc cggccgcgtc
      601 cactagctgc cccggtagga cgtgacgccg tagcgcgaga ggagcagcgg cacgtgcgtg
      661 tgcccgggcg ccagctcgac atcgagcgcg acccgctgga agaacgcgtc cgacgcgtac
      721 gcctgcgtct cgaacacgag ccggtatgtc cccggctcga ggtccttcgc gagctcgcgc
      781 acgcgtccgt cgccgtcggt cacgccctcc gcgacggcgc gcccgccctg ctggagctcg
      841 atgtggactc ccgcggcggg ccggccggcg gtggtgtcga ggacgtgcgt cgagatcgag
      901 gccctggtca tcggcggcgt atcgtacgcc cgccatgcag atgcttcgca tcaccgcggg
      961 gaagtacacg ttcagggcgc ggctggaaga gcagctcgcc ccgcagacct gcgcggccat
     1021 caaaaagtgg ctgccgatcc gcagcaagct cgtgcactgc cgttggagcg gggagtccac
     1081 ctgggtcccg atgggcgaga agcgtctcgg cgtcgagtac gagaaccaca ccagctatcc
     1141 cgcacccggg caggtgctcg tctatcccgg cggcatcagc gagatggaga tcctcttccc
     1201 gtacggctcg acccgtttcg cgagcaaggt cgggcagctc gccgggaacc acttcgcgac
     1261 ggtggtcgag ggcagcgagc acctcgcgga ggtgggacgg ctcacgctct gggaaggcgc
     1321 gcaggacttc acgatcgagc ttgcgtagcc tcgccgtccg cggcggacgc gtcgtgacgt
     1381 ccgccggcgt cgccgccgcc gacgtgctca tcctcgaggg cacgatcacg ggcatcgtcg
     1441 cgccggatgc gggacaggca cgcgaggaga tcgacgcacg cgggctcgtc gtgcttcccg
     1501 gcgtcgtcga cgcgcacgtg cacgtgaacg agccgggtcg caccgagtgg gaggggtggc
     1561 atgccgcgac gcgcggtgcg gccgccggcg gcgtcacgac gatcgcggac atgccgctca
     1621 actccgtgcc gccgacgctc gacggcgcct cgttcgacgc gaagtacgac gcggcatcga
     1681 acggcgcgat cgtcgacttc gcgctctggg gcggcctcgt cgatgccgac gagcgccgcc
     1741 tgcgcgagct cgccgcgtgc ggggtcgtgg gcgtgaaggc gttcacctgc ccgtcgggtg
     1801 tggacgagtt ccctcacatt cgcgacggcg cgctcgcctc cgcgctggcc gcggccacac
     1861 gagccggcct cctcgtcgcc gtccactgcg aggacgaggc caccgtgagc gtgagcaccg
     1921 cgcacctgcg tcgcgccgca cgccgcgacg cgcgcgcgtg gctcgagtcg cgcccgcccg
     1981 aggccgagcg catggcgatc gcgcagctcg cggaggcggc gcgggcgacg agcgcgaagg
     2041 tgcacgtcgt ccatgtgtcg agtcacgagg cgatgcgcgc ggtggaccgc gcgcaacgca
     2101 tcgccgatct caccgcggag acgtgcccgc actatctcgc cttcgacgcc gcggatctcg
     2161 acgagcacgg cgcgctcctg aagtgcgcgc ctccgatccg aagcggggag cgcgacctgc
     2221 tctggcgcga catcctcggc ccgccgccgc ccttccagcg gcgggtcgag tacgtggctt
     2281 ccgatcactc gccatgcagc gccgacctga agacgagggg cgagcgggac atcttcgagg
     2341 cgtggggcgg cgtctccggt ctgcagagcc ttctgccggt catgctcacg gaaggcgtgc
     2401 acgggcgcgg cctcgcgctt cccgagctcg cgcagctcgt cgcgacgcgt cccgcgaagc
     2461 ggctcggcat ctggccgcgc aagggcgaga tccgtggcgg tacggacgcg gacctggtgc
     2521 tcgtggatct ggacgcggaa tggacgctcg atcgcgaggc gctccagacg cgaagtgggc
     2581 tgagccccta cgtcggccgg cggttccggg gtcgcatcgc acggacgatc gtgcgcgggc
     2641 gtacggtcta tgcggatgga acgatcaccg gcgctcccgg cggcggctgg ttcgtgcagc
     2701 gggtgaacgc atgatggcca ccgagctcgt cgccatcgac gagcgtcgcc tcctccggcg
     2761 cctcgaggag ctcgcgcagc gcggcgcgca gacgagcggt ggcatctacc gggcgctgta
     2821 cacgcccgcc tgggtcgcgg cgaccgagct cgtgagcgac tggctcaagg acgcgggtct
     2881 gctcgtgcgc cgcgacgcgg tcgggaacgt ctgggggcgc atcgaaggtg cgcggggcgg
     2941 ccgcgcgatc gtcaccggct cgcacatcga cacggtccgt ggcggcggcg cgctcgatgg
     3001 cgcgctcggc gtggtcgcgg gcatcgaagc ggtcgcgagc ctcgtcgccc gttatggccg
     3061 cccggcgcgc atcctcgagt gcgtggcgat ctgcgaggaa gagggcagcc gcttcaacac
     3121 gaacttctgg ggcgcgcggg cgatcgcgga ccggctcgag cccgatgagg cggcgcgcgt
     3181 gcgcgacgcg gacggcacga gcctggcgga cgcgatgcgc ggcgtcggcc tcgacccgtc
     3241 ccgggcagcg gcggcggccc ggcgcgacat cgacactttc gtcgagctgc acatcgagca
     3301 gggtccgatc ctcgacgacg ctgcggagcg cgtggcggtg gtgcgcacga tcgccggcac
     3361 cgcgcatctc gaggtcgtgg tccgcggcga ggccgaccat gccggggcgg cggcgatgga
     3421 ccgccgccgc gacgcgctcc tgggcgccgc ggcgatgatg ctgcgcatcg ccgaggtcgc
     3481 gcgcgagatg ggaccgccgg cggtggcgac cgtcggcacc atcggcgtcg agcccgcgca
     3541 ggtgaacgtc gttcccggcc tcgcgcgttt caccgtggac gcgcgccaca gcgacgacgc
     3601 gctgcggagg gatctcgtcg ccgacatcga gaaggcttgc cgggtcatcg gccacgagcg
     3661 cgatctccag gtcgaggtgc ggacgctgcg cgaccgtccg ccggtcacgc tcaacgaacg
     3721 caccgcggac gtgctgcgac gcgcatgccg agccgcccgc gtcgagccgc gtgacatgac
     3781 gagcggcgcg gggcacgaca gccagatcct ggtcgccgcg gcgcacaccg ggatgctgtt
     3841 cgtgccgagc atcggcggcc gatcgcatcg cgccgacgag gccacggccg cggcggacgt
     3901 cgtgctcggg acgcgcatcc tcgcgactgc gctccacgag ttggcgtatt agcctccaca
     3961 tccgcggcgc catcctcctg ccgatgaccg acggcgggcg cgccgtgccg ggcgatctgg
     4021 tcgtgtcggg atcgcgcatc gccgccctcg gcccgaacgc gcccgcgccc tcggacgccg
     4081 aggtcctcga ccggcccggc cattacgtca tgcccggtct ggtccagccg cacgtgcatc
     4141 tcgtgcagac gctcttccgc ggtcttgccg aggacctcac gctgctcgac tggctgcagc
     4201 ggcgcgtatg gccgctcgag accgcgcacg acgaggcatc ggtgcgcgcc ggcgtgcggc
     4261 tgggcctcgt cgagctcctg acgtcgggca ccaccacgat cctcgacatg ggcacgacga
     4321 agcacggtga cgcggtcgcc gaggagctcg tgcgctccgg gatccgcgcc tcgttcggcc
     4381 aggcgatgat ggacacgggc gagggcgtcc ctgccggcct cgtggagtcc acccgggcgt
     4441 cactcgacgc gagcgcggcc ctccacaagc ggtggcaccg ctcggccaac ggacgcatcc
     4501 gctacgcgta cgctccgcgc ttcgccctgt cgtgcagccg cgatctcctc gaggcggtgg
     4561 ccgcgctggc gaagatgaac gacctgctcg tgcacacgca ttcgaacgag gatcccggcg
     4621 agcgcgccgc ggtcgagcgg gtgaccggtc gcgcgccgat cgcgtacctc gcggagacgg
     4681 ggatcgcgtc ggaacggacc gtcgcggcgc atggcgtgca cgtcgacgat gccgagctcg
     4741 cgatccttgc ggagacgcgc acggcgatcg cgcactgtcc cacgtcgaat ctgaagctcg
     4801 ccgccggcgt cgccgacgtc gcgcgcctgc gcggcgcacg cgtcaccgtg ggcctcggga
     4861 ccgacggcgc cgcgtgcaac aaccgcctcg atggcttcga ggaggctcgc ctcgcctcgc
     4921 ttctcgcgcg cacgctgcac ggtcagggca cgctctccgc gggcgacgcg ctggtgatgg
     4981 cgacgcgcga gggtgccaag gcgctgcatc tcgacgccga gatcggcacg ctcgaggtcg
     5041 gcaagcgcgc cgacctcgtg atcatcgacc cgtcgcggct cggtggcccg ggcggcgacc
     5101 cggcgacgcg catcgtcttc ggcg
//
LOCUS       DASTZR010000041         3970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_838007, whole genome shotgun sequence.
ACCESSION   DASTZR010000041 DASTZR010000000
VERSION     DASTZR010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838007"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="VFV20_00940"
     CDS             complement(<1..652)
                     /locus_tag="VFV20_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5286945.1"
                     /translation="MALLEVSGLVRRFRGLAAVDDLSFTVDEGKTFAIIGPNGAGKST
                     TFNLVSGLLATHGGSIRFRGEDVTRRPAHLRARAGLGRTFQVVQPFVDLDVMDNVIVG
                     ALFGASADLTAARAEAERLCAFVGLGEHLHRPVASLTLADRKRLELARALATKPALLL
                     LDEVMEGLTPAEERDAIALLGRIRAEGITLLLIEHVMSTVRDLSDRVLVMDYGKKLA"
     gene            complement(634..1578)
                     /locus_tag="VFV20_00945"
     CDS             complement(634..1578)
                     /locus_tag="VFV20_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015716806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU5286946.1"
                     /translation="MRRDIVGWVVFLAIVAMLPATGIGSDRYLMLLANVMLMFMTLAL
                     SWDVVARTGQLSLAHGAFFGLGAYSVALAFKLWAVPPLVGLVFAAIAVAVIAFALGIV
                     TLRLRGIYFAIATLAFAEVWRTVALQVPDLSGGTVGIAMPPLFGGDRILSYYVIGAIF
                     VLALGLSLAVRWSRWQYAVTAVRANETVARVMGLDVVRVKVGLFTASATLAGLAGGFY
                     MPFITHTDPYDAFDIGRSVAALVFPIFGGLYTAAGPVIGTIVMRSLEEYLRVTPPWKD
                     GYQVLYGLVIVVAVLFMPRGVMGLAEPYLRRLPWRSSR"
     gene            complement(1601..2494)
                     /locus_tag="VFV20_00950"
     CDS             complement(1601..2494)
                     /locus_tag="VFV20_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015235820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU5286947.1"
                     /translation="MEALPQTLLFGILRSGLYALTALGLAISLGVIGVVNFAHGEFVM
                     LGAYIGFWLYMLLSGPLGLGPAVSPLVIAVGIPLSAVALYLVATGVYRLTLRPVLRQP
                     ELNQMLLTFGLSILLVNGASITLTPQAKDANLDWRFDVLDLGFASVTYARLAVFAVCI
                     ALVLASYLLLMRSRLGKQMRAVAQNRLGAQIVGIHVDRVYEIAFGMSAALAAAAGVML
                     VFQTQVSPFVGLGTLLKAFSIVVLAGLGNLTGVLWASLLLGLAEQVVSQYVPGGSALR
                     DGLFFVIIFVVLLARPGGLRR"
     gene            complement(2521..3750)
                     /locus_tag="VFV20_00955"
     CDS             complement(2521..3750)
                     /locus_tag="VFV20_00955"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5286948.1"
                     /translation="MRINRIVTGLVAVGLLAGACGGTPAASPTPSAAASSAAAATAAP
                     KPPIKLGALLPLTGPSAATGQDMKDGYELAVEQINAAGGVNGAKVQVIYEDDKNDPAT
                     AVASWEKLVNSDKVEIMMGGLASTISLALVEPAKKAAIPMAWTGAAATAFEQGMANEP
                     YFFHYHPWEYQNGLSAYAFLKSTPIKKWALVYEDGAFGTGAAKGFKEDFPKQAPGAEV
                     VASEPFKTGSTDFTALLNRVKASGAEGLILVPFAGDVIPFLTQMREVAYKPKLVYASP
                     PSFPPDFAKSPVAEGVSGLTLWTADIPAPASKKFVADFTKKYNHAPVSYWSALAYTNI
                     VTATNALAKSGSGNKAGWISAMEATSYDSPLGPTLKFTPSQFTKHQGFTSLVSFQFQS
                     GKQEIVFPENLSTAKLK"
     gene            complement(3795..>3970)
                     /locus_tag="VFV20_00960"
     CDS             complement(3795..>3970)
                     /locus_tag="VFV20_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEU5286949.1"
                     /translation="NASATADELTALCRSRLAKYKTPRYVVFVNALPRNAAGKVLKRQ
                     LREEVRVREQINT"
BASE COUNT          660 a         1342 c         1385 g          583 t
ORIGIN      
        1 cggcgagctt cttcccgtag tccatgacga gcacccggtc cgacagatcg cggaccgtgc
       61 tcatcacgtg ttcgatgagc agcagcgtga tgccctcggc ccggatccgg ccgagcagcg
      121 cgatcgcgtc acgctcttcg gccggcgtga ggccctccat gacctcgtcc agcagcagga
      181 gcgccggctt cgtcgcgagc gcgcgtgcga gctcgaggcg cttgcggtcg gcgagcgtga
      241 gcgaggcgac cggacggtgt aggtgctcgc ccagaccgac gaacgcgcac aggcgttcgg
      301 cctcggcgcg tgcggcagtg aggtcggcgc tcgccccgaa gagcgccccg acgatgacgt
      361 tgtccatgac gtcgaggtcg acgaacggct gcacgacctg gaacgtgcgg ccgaggccgg
      421 cgcgcgcgcg caggtgcgcc ggccgtcggg tgacgtcttc gccgcggaac cggatgctgc
      481 cgccgtgtgt ggcgaggagg ccggaaacga gattgaaggt cgtggacttc ccggccccgt
      541 tcggcccgat gatcgcgaac gtcttgccct cgtccaccgt gaatgagagg tcgtcgaccg
      601 cggccaggcc gcggaagcgc cgcacgagtc cgcttacctc gaggagcgcc atggcaacct
      661 ccgcaggtac ggctcggcca ggcccatgac gccgcgcggc atgaagagga cggcgaccac
      721 gatgaccagg ccgtacagga cctggtagcc gtccttccac ggcggcgtca cgcgcaggta
      781 ctcctcgagc gagcgcatga cgatcgtgcc gatgaccgga cccgcggccg tgtacaggcc
      841 gccgaagatc gggaacacca gcgcggccac ggagcggccg atgtcgaacg cgtcgtacgg
      901 atccgtgtgc gtgatgaacg gcatgtagaa cccgcccgcg agaccggcga gcgtcgcgga
      961 ggcggtgaac aggccgacct tcacgcgcac gacgtccagg cccatcacgc gcgccaccgt
     1021 ctcgttggcg cgcacggcgg tgaccgcata ctgccatcgc gaccagcgca ctgcgagcga
     1081 gagcccgagc gcgagcacga agatcgcgcc gatgacgtaa taggacagga tccgatcgcc
     1141 gccgaagagc ggcggcatgg cgatcccgac cgtcccgccc gagaggtccg gcacctggag
     1201 cgccaccgtg cgccacacct cggcgaaggc gagcgtcgcg atcgcgaagt agatgccgcg
     1261 caatcggagc gtcacgatgc cgagcgcgaa cgcgatgacg gccaccgcga tcgccgcgaa
     1321 gacgagcccg acgagcggcg gcaccgccca gagcttgaac gcgagcgcga cgctgtacgc
     1381 gccgagcccg aagaacgcgc cgtgcgcgag cgacagctgg ccggtgcgcg cgacgacgtc
     1441 ccaggagagc gcgagcgtca tgaacatgag catcacgttc gcgaggagca tcaggtaccg
     1501 atcgctgccg atgccggtgg cggggagcat cgccacgatc gcgaggaaga cgacccagcc
     1561 gacgatgtcc ctcctcatat cgtcgcgctc gcctcctgct tcatcgccgc agcccacccg
     1621 gccgcgccag caggaccacg aagatgatca cgaagaagag cccgtcgcgc agggcggatc
     1681 cgccgggcac gtattgcgac acgacctgct cggcaaggcc cagcagcagg ctcgcccaca
     1741 ggacgccggt gagattcccg aggccggcga gcaccacgat cgagaacgcc ttgagcagcg
     1801 tgccgaggcc cacgaacggg ctgacctgcg tctggaacac gagcatcacg ccggccgcgg
     1861 ccgcgagcgc cgcggacatg ccgaacgcga tctcgtagac gcggtcgacg tggatgccga
     1921 cgatctgcgc gccgagccgg ttctgcgcga ccgcgcgcat ctgcttcccg agccggctgc
     1981 gcatgagcag caggtagctc gcgagcacga gcgcgatgca gacggcgaag accgccagac
     2041 gcgcgtacgt gacgctcgcg aagccgaggt cgaggacatc gaagcgccag tcgaggttcg
     2101 cgtccttcgc ctgcggcgtg agcgtgatgc tcgcgccgtt cacgagcagg atcgagaggc
     2161 cgaacgtgag gagcatctgg ttgagctcgg gctggcggag caccggacgc agcgtcaggc
     2221 ggtacacgcc cgtcgcgacg aggtacagcg ccaccgccga cagcgggatg ccgacggcga
     2281 tgacgagcgg gctcaccgcg ggcccgagcc ccagcggacc cgacaggagc atgtagagcc
     2341 agaagccgat gtacgcgccg agcatcacga actcgccgtg cgcgaagttc acgacgccga
     2401 tgacgccgag cgagatggcg aggccgaggg cggtgagggc gtagaggccg gaacgcagga
     2461 tcccgaacag cagggtctgc ggcagcgcct ccacgccctc tccctattcc tttgttctga
     2521 ctacttcagc ttcgcggtgg acaggttctc ggggaagacg atctcttgct tcccggactg
     2581 gaactggaag gacacgaggc tcgtgaagcc ctgatgcttc gtgaactggc tcggcgtgaa
     2641 cttgagcgtc gggcccagcg gcgagtcgta gctcgtcgcc tccatcgcgc tgatccatcc
     2701 ggccttgttg ccgctcccgg acttcgccag cgcgttggtg gccgtgacga tgttcgtgta
     2761 cgcgagcgcc gaccagtagc tgaccggcgc gtggttgtac ttcttcgtga agtccgcgac
     2821 gaacttcttc gacgccggcg cggggatgtc cgcggtccac agcgtgagac ccgagacgcc
     2881 ctccgcgacg ggcgacttgg cgaagtccgg cgggaacgat ggcggtgatg cgtagaccag
     2941 cttcggcttg taggcgacct cgcgcatctg tgtgaggaac gggatcacgt cgccggcgaa
     3001 cgggacgagg atgaggccct cggcgcccga cgccttcacg cggttcagga gcgcggtgaa
     3061 gtcggtcgag cccgtcttga acggttcgct cgcgacgacc tccgcacccg gagcctgctt
     3121 ggggaagtcc tccttgaagc ccttggccgc gccggtgccg aacgcgccgt cttcgtagac
     3181 gagcgcccac ttcttgatcg gcgtcgactt caggaacgcg tatgccgaca ggccgttctg
     3241 gtactcccag gggtggtagt ggaagaagta cggctcattc gccatgccct gctcgaaggc
     3301 cgtggcggcg gcgccggtcc acgccatcgg gatggccgcc ttcttcgccg gctcgacgag
     3361 cgcgaggctg atggtcgacg cgaggccgcc catcatgatc tcgaccttgt cgctgttgac
     3421 gagcttctcc cacgacgcga ccgccgtggc gggatcgttc ttgtcgtctt cgtagatcac
     3481 ctggaccttg gcgccgttga cgccgccggc cgcgttgatc tgctcgacgg cgagctcata
     3541 gccgtccttc atgtcctggc ccgtcgccgc cgacggtccg gtgagcggaa gcagcgcgcc
     3601 gagcttgatc ggcggcttcg gcgccgcggt cgcggccgcc gctgacgatg ccgcggccga
     3661 cggggtcggc gacgccgcgg gcgtgccgcc gcaggctccg gcgagcaggc cgaccgcgac
     3721 gaggccggtc acgatgcgat tgatgcgcat ggtccgtgct cctcccatgt gtgctgtttg
     3781 agttcgcgcg ttccctacgt gttgatctgc tctcgtaccc gcacctcctc acgcagctgt
     3841 ctcttcagca ccttccccgc ggcgttgcgc gggagggcgt tcacgaaaac gacgtaccgc
     3901 ggcgtcttgt acttcgcgag ccgtgacctg cagagcgcgg tgagctcgtc cgcggtcgcg
     3961 ctcgcgttgg
//
LOCUS       DASTZR010000042         2094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_577434, whole genome shotgun sequence.
ACCESSION   DASTZR010000042 DASTZR010000000
VERSION     DASTZR010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577434"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..547)
                     /gene="rplK"
                     /locus_tag="VFV20_00965"
     CDS             complement(122..547)
                     /gene="rplK"
                     /locus_tag="VFV20_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEU5286950.1"
                     /translation="MATKIKAVVKVQIPAGKATAAPPVGTALGPHGINMGQFIKDYNE
                     RTASLAGTVVPAVVTIFEDRSFTFVTKSPPAADLIRKEAGLEKGSGKPNKEKVGKITK
                     AAVKKIAETKMNDLNATSIESAMKMVEGTARSMGVEIAG"
     gene            complement(547..1059)
                     /gene="nusG"
                     /locus_tag="VFV20_00970"
     CDS             complement(547..1059)
                     /gene="nusG"
                     /locus_tag="VFV20_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEU5286951.1"
                     /translation="MIHTYSGYENKVKTNLEHRIQSMDMGDKIFQVLVPTEEEIEIKN
                     GKRHPVERKIFPGYVLVEMVMSDDSWYVVRNTPGVTSFVGSGNKPTELNDTEVRQILR
                     QIKLDAPKYKVAFTKGEAVRVTDGPFTDLHGVVDEVNPERNKVKVLVSIFGRETPVEL
                     DFLQIEKLVK"
     gene            complement(1396..1638)
                     /gene="secE"
                     /locus_tag="VFV20_00975"
     CDS             complement(1396..1638)
                     /gene="secE"
                     /locus_tag="VFV20_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEU5286952.1"
                     /translation="MANAPALPRGGGLVNFAQECWSELQKVTWPERETIVRLTVIVIL
                     ISALIAAYIFLFDNLFTFTITQNFVGAPTASPAPAP"
     gene            complement(1655..1727)
                     /locus_tag="VFV20_00980"
     tRNA            complement(1655..1727)
                     /locus_tag="VFV20_00980"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1692..1694),aa:Trp,seq:cca)
     gene            complement(1783..1944)
                     /gene="rpmG"
                     /locus_tag="VFV20_00985"
     CDS             complement(1783..1944)
                     /gene="rpmG"
                     /locus_tag="VFV20_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU5286953.1"
                     /translation="MAKDNRPIITLACTTCRSRTYSTTKNRKNDPDRLEMPKFCPRCR
                     RHTPHRETR"
     gene            complement(1952..>2094)
                     /gene="tuf"
                     /locus_tag="VFV20_00990"
     CDS             complement(1952..>2094)
                     /gene="tuf"
                     /locus_tag="VFV20_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019550494.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU5286954.1"
                     /translation="EMVMPGDNANLTITLITPIALEEGLRFAIREGGHTVGAGVVTKI
                     LE"
BASE COUNT          289 a          685 c          693 g          427 t
ORIGIN      
        1 gttcagcatc ccggccgagg gcggcaaggt gcgcgtgcaa ggcagcgcga ccgtcgaccg
       61 cggaacgttc gtcgcggacg ggaccgcgga gggtcgcccg gcaaagtagc cgcgcgagtg
      121 gttagccggc gatctccacg cccatgctgc gcgcggtgcc ctcgaccatc ttcatggccg
      181 actcgatgct cgtcgcgttc aggtcgttca tcttggtctc ggcgatcttc ttcaccgcgg
      241 ccttcgtgat cttgccgacc ttttccttgt tcggcttgcc ggagcccttc tcgaggccag
      301 cctccttgcg gatgaggtcg gccgcgggcg gcgacttggt gacgaacgtg aacgagcggt
      361 cctcgaagat ggtgacgacc gccggcacca ccgtcccggc gagcgacgcg gtgcgctcgt
      421 tgtagtcctt gatgaactga cccatgttga tgccgtgcgg gccgagcgcg gtgccgacgg
      481 gcggcgccgc ggtggccttg cccgcgggga tctggacctt gacgacggcc ttgatcttcg
      541 tcgccattac ttcaccaact tctcgatctg caggaagtcc agctccacgg gcgtctcgcg
      601 tccgaagatg gacaccagga ccttcacctt gttgcgctcg gggttcacct cgtcgacgac
      661 accgtgcagg tcggtgaagg gaccgtcggt gacgcgcacc gcttcgccct tcgtgaacgc
      721 gaccttgtac ttcggcgcgt cgagcttgat ctgccgcagg atctggcgga cctcggtgtc
      781 gttgagctcg gtcggcttgt tgccgctacc gacgaagctc gtgacgcccg gcgtgttccg
      841 cacgacgtac caggagtcgt cgctcatgac catctcgacg agcacgtaac ccgggaagat
      901 cttgcgctcc accgggtggc gcttgccgtt cttgatctcg atctcttcct cggtcggcac
      961 gagcacctgg aagatcttgt cgcccatgtc catggattgg atgcggtgct ccaggttcgt
     1021 cttcaccttg ttctcgtagc ccgagtaggt gtggatcacg aaccagcggc gaccgtcctc
     1081 ggtcgtcccc gcgggggtcg gcgcggcggg ggcctcgggc tccgggacgg gcgcctcgac
     1141 cacttcgggg gccggctcga cctcggcgac cgcaccggca cggcgcagga ggttcttggc
     1201 ctcgtctacc gagacctcct cctcgggctc cgcggtgccg aggaacgcgc gcgcggcctg
     1261 tgccgcggcc tcgtccgaca gcttggcggc gtcgatctcc tctttcgggg cgacgtcgct
     1321 ctgcggccgg tcctcggtcg tctcgatcgt ctcgtccttg ttgtcttgct cgtccactgg
     1381 ctactgcctc cgggtctacg gtgcgggcgc ggggctggcc gtcggcgctc cgacgaagtt
     1441 ctgcgtgatc gtgaacgtga acaggttgtc gaacaggaag atgtaggccg cgataagggc
     1501 ggagatcagg atgacgatga ccgtgagacg gacgatcgtc tcgcgctcag gccaggtcac
     1561 cttctggagc tccgaccagc actcctgcgc gaaattgaca aggccaccgc cacgcggcag
     1621 cgcgggtgcg ttcgccatta ccggcctccc tctgcacggc gggagagact cgaactctca
     1681 accactggtt ttggagacca gggctctgcc agttgagcta ccgccgtatg cggcgcctcg
     1741 gcgccgcata cggcggacga ggctccattc cttctcattg tctcaacgag tctcccggtg
     1801 cggcgtatgc ctgcggcacc gtggacagaa cttaggcatc tcgagtcggt ccggatcgtt
     1861 cttgcggttc ttcgtcgtcg agtacgtgcg cgaccggcag gtcgtgcacg cgagcgtgat
     1921 gatcggccgg ttgtcctttg ccatcctctt cttactccag gatcttcgtc acgacacccg
     1981 cgcccacggt gtggccgcct tcgcggatgg cgaagcgcag gccctcctcc agggcgatgg
     2041 gggtgatgag cgtgatcgtg aggttggcgt tgtcgccggg catgaccatc tcgg
//
LOCUS       DASTZR010000043         2609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_54364, whole genome shotgun sequence.
ACCESSION   DASTZR010000043 DASTZR010000000
VERSION     DASTZR010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54364"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="VFV20_00995"
     CDS             complement(<1..1027)
                     /locus_tag="VFV20_00995"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEU5286955.1"
                     /translation="MNRNRAVAVGAFVVGGVLLFALGLFLIGDRRMLFEKNFEVYAEF
                     AKIAGLQNGAIVRVAGMDAGEVETIHIPRAPGQPFRVKLRVREDLHPLIRLDSIASIQ
                     NDGLVGNKFVQVEAGTEQSPTAPDRGTIQSREPFDIADMMQRMNETIETVNTIVADVK
                     GSVEEALTSITSTAKDAQALIHDVGDEMQSIMTSTQRVARNVNEIVAGVRAGRGTVGQ
                     LLTDDSLYRSAKNIAAEAEKAMATVRDASEQARAAIADFRGEGGPMKGVTADVQQTLV
                     QAREAMTDLAENTEALKRNFFFRGFFNRRGYFDLNDVSVDQYRQGVLASKDRRALRIW
                     VASAVIFE"
     gene            complement(1029..1766)
                     /locus_tag="VFV20_01000"
     CDS             complement(1029..1766)
                     /locus_tag="VFV20_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5286956.1"
                     /translation="MATVRLEGVCKTFGARRVLDDVSFTVEDGCGFVILGRSGTGKSV
                     TLKHIIGLIRPDRGRVFVDDDQINELGAAQLSSARKKIGFLFQNAALFDSISVGENVA
                     FPLRRHTEMSDSEIRARATEKLDAVGLGHEYDKMPADLSGGMRKRAGLARALALDPPI
                     LLVDEPSAGLDPITADEIDELLLRLKESGGTTLIVVTHNIASARRLGDELLMLHDGRV
                     VARGTPAEFDRSEHELVRAFMSSTHAG"
     gene            complement(1766..2551)
                     /locus_tag="VFV20_01005"
     CDS             complement(1766..2551)
                     /locus_tag="VFV20_01005"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5286957.1"
                     /translation="MTARASAVASSWLSSVGSYALFAARAARASVTPPFEFRETIRQI
                     YELGFRSAPLIAVSGLAVGVVLSMHTRASLERFGAESMIPAGLAMALVRETGPLTAGL
                     LLSGRIGAGIGAELGAMRVTEQIDALEASAVDAFRFLVVTRVIACVIALPILTTIMNF
                     AGILGGFLAETAASGMSLQLYFTRAFSMIDMTDYIPATLKTMVFGFIIATVSSYLGFT
                     TESGTEGVGRASTRAVVLSSIMIIVVNVILVRLIFFVFPQAAG"
BASE COUNT          413 a          911 c          872 g          413 t
ORIGIN      
        1 tctcgaaaat gaccgccgag gcgacccaga tgcgcagcgc gcggcggtcc ttcgacgcca
       61 gcaccccctg gcggtactgg tccacgctga cgtcgttcag gtcgaagtac ccgcgtcggt
      121 tgaagaagcc ccggaagaag aagttgcgct tgagcgcctc ggtgttctcg gcgagatcgg
      181 tcatcgcctc gcgcgcctgg acgagcgtct gctggacgtc cgccgtcacc cccttcatcg
      241 gcccgccttc cccgcggaag tcggcgatcg cggcgcgcgc ctgctcgctc gcgtcccgca
      301 ccgtcgccat ggccttctcg gcctccgcgg cgatgttctt cgcgctccga tacagcgagt
      361 cgtcggtcag cagctggccc accgtgccgc gcccggcccg cacgccggcg acgatctcgt
      421 tcacgttccg agcgacgcgc tgcgtcgagg tcatgatcga ctgcatctcg tcgccgacat
      481 cgtggatgag cgcctgcgcg tccttggccg tggaggtgat cgacgtgagc gcctcctcga
      541 ccgagccctt cacgtccgcg acgatcgtat tgacggtctc gatcgtctcg ttcatccgct
      601 gcatcatgtc ggcgatgtcg aacggctccc ggctctggat cgtgccgcga tcgggcgccg
      661 tcggcgactg ttcggtgccc gcctccacct gcacgaactt gttgccgacg aggccgtcgt
      721 tctggatcga ggcgatcgag tcgagccgga tcagcgggtg caggtcctcg cgcacccgga
      781 gcttgacgcg gaacggctgg cccggcgccc gcgggatgtg gatcgtctcg acttcgccgg
      841 cgtccatccc cgccacgcgc acgatcgccc cgttctggag gccggcgatc ttcgcgaact
      901 cggcgtagac ctcgaagttc ttctcgaaga gcatccggcg atcgccgatc aggaagaggc
      961 ccagcgcgaa gaggagaacg ccccccacca cgaatgcgcc gacggcgacg gcccggtttc
     1021 ggttcatctc accctgcgtg cgtcgaggac atgaaggcgc ggacgagctc gtgctcgctg
     1081 cgatcgaact ccgcgggcgt cccgcgggcg acgacgcgcc cgtcgtgcag catcaggagc
     1141 tcgtcgccga ggcgccgcgc gctcgcgatg ttgtgcgtca cgacgatcag cgtcgtgccg
     1201 ccgctctctt tcaggcgcag gaggagctcg tcgatctcgt cggccgtgat cggatcgagg
     1261 ccggcgctcg gctcgtccac caggaggatc ggcgggtcca gcgcgagcgc gcgcgccagc
     1321 cccgcccgct tgcgcatgcc gccggacagg tccgccggca tcttgtcgta ctcgtgtccg
     1381 aggccgaccg cgtcgagctt ctcggtggcg cgggcgcgga tctccgagtc gctcatctcg
     1441 gtgtggcggc gcagggggaa cgcgacgttc tcgccgaccg agatcgagtc gaacagcgcg
     1501 gcgttctgga acaggaaccc gatcttcttc cgcgcgctcg agagctgcgc ggcgcccagc
     1561 tcgttgatct ggtcgtcgtc cacgaacacc cgtccgcggt ccggacgaat caggccgatg
     1621 atgtgcttca gcgtgacgct cttccccgtc ccgctgcgtc cgaggatcac gaacccgcac
     1681 ccgtcttcca ccgtgaacga cacgtcgtcc agcacgcgcc gcgcgccgaa cgtcttgcac
     1741 acgccttcga gccgtaccgt cgccattacc ctgccgcctg cgggaagacg aagaagatca
     1801 gccgcacgag gatcacgttg acgacgatga tcatgatcga ggagagcacc accgcgcgcg
     1861 tggacgcgcg gcccaccccc tcggtgccgg actcggtcgt gaagcccagg taggaggaca
     1921 cggtcgcgat gatgaagccg aacaccatcg tcttcagcgt ggccgggatg tagtcggtca
     1981 tgtcgatcat cgagaacgcg cgcgtgaaat acagctggag ggacatgccg ctggcggccg
     2041 tctcggcgag gaacccgccg aggatgcccg cgaagttcat gatagtcgtc aggatgggca
     2101 gcgcgatcac gcaggcgatg acgcgtgtga ccaccaggaa gcggaaggca tccaccgccg
     2161 acgcttcgag cgcgtcgatc tgctcggtga cgcgcatcgc cccgagctcg gcgccgatgc
     2221 cggcgccgat gcggcccgac aggagcagtc ccgccgtcag ggggccggtt tcccggacga
     2281 gcgccatcgc cagaccggcg ggaatcatcg attcggcccc gaagcgctcg agcgacgccc
     2341 gcgtgtgcat cgacagcacg acgccgacgg caaggccgga gaccgcgatc aagggggcgg
     2401 agcggaatcc cagctcgtag atctgccgga tcgtctcgcg gaattcgaac gggggggtga
     2461 cgctcgcgcg cgccgcgcgg gcggcaaaca gcgcgtagct gccgaccgac gacagccagg
     2521 aggaggcaac ggcgctagct cgagcagtca atggccgagc tgattgcaag gttcacaccc
     2581 tactgggcca ccgcccggcc cttgctgtc
//
LOCUS       DASTZR010000044         2531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_995160, whole genome shotgun sequence.
ACCESSION   DASTZR010000044 DASTZR010000000
VERSION     DASTZR010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995160"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..>2531
                     /locus_tag="VFV20_01010"
     CDS             53..>2531
                     /locus_tag="VFV20_01010"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5286958.1"
                     /translation="MGSDGLSAFHPAVQEWFRTSFPSPTPAQAQAWPVIKGGESTLLM
                     APTGSGKTLAAFLSSIDELVRAPARGCRVLYVSPLKALAVDVERNLRAPIVGIARAAE
                     RLGVAVREPTVAIRTGDTPARERARFVKTPADIFITTPESLYLVLSSSAGEALRDVET
                     VIVDEIHAIVSTKRGAHLAVTLERLERHVAERLRRTGRQRLQRIGLSATQRPLDEVAR
                     FLGGFEDAGPRPVQVVDAGHRKPLELSVEVPVEDMARLGDTVEFRSGPAARADARTSI
                     WPAIHPRLLELVRAHRSTLVFVNSRRLAERIAAALNELAGEEIAHAHHGSIAREQRLR
                     IEDDLKAGRLRALVATSTLELGIDMGAIDLVVQIESPPSVASGMQRIGRAGHRVDEPS
                     TGIILPKYRGDLLASAALTERMHAGAVEVSRYIRNPLDVLAQHIVAMVGREAMAVDEL
                     ERVVRGAAPYAELPREQLENVLDMLSGHYPSDEFVELRPRIVWDRIAGTVRAREGAAR
                     VALTSGGTIPDRGLYGVFLVGADPGKGRVGELDEEMVFETRVGETFLLGASTWRVEEI
                     THDRVLVSPAPGVPGKMPFWKGETATRPLEFGRAIGALTRELRAADPEAASKTLRERH
                     DLDERAAKNLLEYLRDQMAAAGAVPDDRTILVERYLDEVGDWRMCVLSPFGGKVHAPW
                     AMAIGAMVRERSDVEIDVLWTDDGIVARFPEAERAPDPSVVIPDPERVEAMVIERLSQ
                     TPLFAARFREAAARALLLPRRFPGRRSPLWHQRRRAGELLQVASRFSTFPIILEAYRE
                     CLQDVFDMPALLEVLRAIARREIRIVTV"
BASE COUNT          328 a          936 c          908 g          359 t
ORIGIN      
        1 gtgtgttggg aacgacgcgg tcacgcgtcg cggcagcgga tatcgtctcg tcatggggag
       61 cgacggtctg tcggcgttcc atccggccgt ccaggagtgg ttccggacgt cgttcccgtc
      121 gccgacgccc gcgcaggcgc aagcgtggcc ggtcatcaag ggcggcgaga gcacgctgct
      181 catggcaccc accgggtccg gcaagaccct cgcggcgttc ctctcgagca tcgacgagct
      241 cgtgcgcgcg ccggcgcgcg gctgccgggt cctgtacgtc tcgccgctca aggcgctcgc
      301 cgtggacgtg gagcgcaacc tgcgcgcgcc catcgtgggc atcgcgcgcg cggcggagcg
      361 tctcggcgtc gccgtgcgcg agccgaccgt cgcgatccgc accggcgaca cgcctgcgcg
      421 cgagcgcgca cgcttcgtga agacgccggc cgacatcttc atcaccacgc cggagtcgct
      481 ctatctcgtg ctctccagtt ccgccggcga ggcgctgcgc gacgtggaga ccgtgatcgt
      541 cgacgagatc cacgcgatcg tctcgaccaa gcgcggcgcg catctcgcgg tcacgctcga
      601 gcgcctcgag cgccacgtcg ccgagcgcct tcgccgaacg ggacgtcagc ggctgcagcg
      661 catcgggctc tccgcgacgc agcgcccgct cgacgaggtc gcgcgattcc tgggcggctt
      721 cgaggacgcg ggtccgcgcc cggtccaggt cgtcgacgcg ggacaccgca agccgctcga
      781 gctcagcgtg gaggtcccgg tcgaggacat ggcgcggctc ggcgacaccg tcgagttccg
      841 cagcggcccc gccgcccgcg cggacgcccg caccagcatc tggccggcca tccacccgcg
      901 gctgctcgag ctcgtgcgcg cgcatcgctc gacgctcgtc ttcgtcaact cgcgccggct
      961 cgccgagcgc atcgccgccg cgctgaacga gctggcgggg gaggagatcg cgcacgcgca
     1021 ccacggttcg atcgcgcgcg agcagcgtct gcggatcgag gacgatctca aggccggccg
     1081 cctgcgcgcg ctcgtggcga cgagcacgct cgagctcggc atcgacatgg gcgcgatcga
     1141 cctcgtcgtg cagatcgaat cgccgccatc ggtcgccagc ggcatgcagc gcatcggccg
     1201 cgccggtcac cgcgtcgacg agccgtccac cgggatcatc ctgccgaagt accgcggcga
     1261 cctcctcgcc tcggccgcgc tcaccgagcg gatgcacgcc ggggcggtcg aggtctcgcg
     1321 ctacatccgc aacccgctgg acgtcctcgc gcagcacatc gtggcgatgg tcggccgcga
     1381 ggcgatggcc gtcgacgagc tcgagcgcgt cgtgcgcggg gcggcgccgt acgcggagct
     1441 gccgcgcgag cagctcgaga acgtgctcga catgctgtcg ggccactacc cctccgacga
     1501 gttcgtcgag ctgcgcccgc gcatcgtgtg ggaccgcatc gcggggacgg tgcgcgcgcg
     1561 cgagggcgct gcgcgggtgg cgctcacgtc gggcggcacc atcccggacc gcggcctcta
     1621 cggcgtgttc ctcgtcggcg cggatcccgg caagggccgc gtcggcgagc tcgacgagga
     1681 gatggtcttc gagacgcgcg tcggcgagac gttcctcctc ggcgcatcca cgtggcgcgt
     1741 ggaggagatc acgcacgatc gcgtgctcgt ctcgcccgcg ccgggcgtac ccggcaagat
     1801 gccgttctgg aagggggaga ccgcgacacg tccgctcgag ttcggccgcg cgatcggcgc
     1861 gctcacgcgc gagctgcgcg cggcggatcc ggaggccgct tcgaagaccc tgcgcgagcg
     1921 gcacgacctc gacgagcgcg ccgcgaagaa cctgctcgag tacctgcgcg accagatggc
     1981 ggcggcgggc gccgtccccg acgaccgcac catcctcgtc gagcgctacc tcgacgaggt
     2041 cggcgactgg cgcatgtgcg tcctctcgcc gttcggcggc aaggtccacg cgccgtgggc
     2101 gatggcgatc ggcgcgatgg tgcgcgagcg atcggatgtc gagatcgacg tcctctggac
     2161 cgacgacggc atcgtcgcgc gcttccccga ggcggagcgc gcgccggacc cgtccgtcgt
     2221 catccccgac ccggagcgcg tcgaggcgat ggtgatcgag cggctctcgc agacgccgct
     2281 cttcgccgcg cgcttccgtg aggccgccgc gcgcgcgctg ctcctgccac ggcgcttccc
     2341 cggacggcgc tcgccgctct ggcatcagcg ccggcgcgcg ggcgagctgc tgcaggtggc
     2401 gtcgcgcttc tcgacgttcc cgatcatcct cgaggcctat cgcgagtgtc tgcaggacgt
     2461 cttcgacatg cccgcgctgc tcgaggtgct ccgtgcgatc gcgcgacgtg agatccgcat
     2521 cgtcaccgtc g
//
LOCUS       DASTZR010000045         2090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1047647, whole genome shotgun sequence.
ACCESSION   DASTZR010000045 DASTZR010000000
VERSION     DASTZR010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047647"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /locus_tag="VFV20_01015"
     CDS             <1..1118
                     /locus_tag="VFV20_01015"
                     /inference="COORDINATES: protein motif:HMM:NF019952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HEU5286959.1"
                     /translation="GIAGALLVDATWRAEPRGDVGLFEVDEASLPRLLGRIKPRVLVV
                     TNLFRDQLDRYFEIDQLARRIGDAIATLDPACVVVLNADDPLVAMLGERHPGTVVYFG
                     VDDPSVGGRVPQVISDATRCPRCRAPLHYERVILAHEGEWSCPSCGLARPPRDVSAIA
                     VTLGSSSSELRLRLPAAGAFDPIRVPLPGLYNAYNALAAIAVSRALDIALPTATRAIG
                     SFKPAFGRLEQISVDGRSVRLVLVKNPAGFNAAIGALLETGREPRILAALNDRDADGR
                     DVSWIWDADFESLAPRVEHAVVTGIRSRDLANRLKYAGVPRERLEAVDDTASAIDRAI
                     GATPREEELIVLATYTAMLAIRAELARRGHVAPFWED"
     gene            1122..1556
                     /locus_tag="VFV20_01020"
     CDS             1122..1556
                     /locus_tag="VFV20_01020"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEU5286960.1"
                     /translation="MPGMPRFTKTPPDVLAAFEAARPTRRDVDRRTMFGYPALFVRGN
                     MFAFTFGPKVAVRLGASARARAATAGAAAFEVMPGRAMTEYVAVPPSAMKGAALKKWL
                     ADALAYADTLPPKGAAKTLRKTTVKTATKTTAKTAKRTAAKR"
     gene            1556..>2090
                     /locus_tag="VFV20_01025"
     CDS             1556..>2090
                     /locus_tag="VFV20_01025"
                     /inference="COORDINATES: protein motif:HMM:NF019305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEU5286961.1"
                     /translation="MDLRIAHLYPDLMSIYGDRGNVLALCQRARWRGIDAEVRAYSAG
                     TSFDADWPDLLFFGGGQDQGQDIVGADLQGPNGDAVRRAIAGGAAALTICGGYQLFGV
                     EYVPENASAIPGLGVLDVRTRAGKRRFVGNLVVETPDGTLVGFENHSGRTYLGPKALP
                     LGAVLVGHGNNGEDRTEG"
BASE COUNT          286 a          755 c          742 g          307 t
ORIGIN      
        1 ggggcatcgc gggcgcgctc ctcgtggacg cgacctggcg cgccgaaccg cgcggcgacg
       61 tcggcctctt cgaggtcgac gaggcgtccc tgccgcgcct cctcggccgc atcaagccgc
      121 gcgtcctcgt ggtcacgaat ctcttccgcg accagctcga tcgctacttc gagatcgatc
      181 agctcgcccg ccggatcggt gatgcgatcg cgacgctcga tcccgcatgc gtcgtcgttc
      241 tcaacgccga cgatccgctc gtcgcgatgc ttggtgagcg tcatccgggc accgtcgtgt
      301 acttcggggt cgacgaccca tccgtcggcg gtcgggtgcc gcaggtgatc agcgacgcga
      361 cgcgctgccc gcgctgccgg gcgccactgc actacgagcg cgtcatcctc gcccatgagg
      421 gcgagtggtc ctgcccgtcg tgcggtctgg cgcggccgcc gcgcgacgtg tccgcgatcg
      481 cggtgacgct ggggtcgtcg tcgagcgagc tgcggctgcg gctccccgcg gcgggggcat
      541 tcgatccgat ccgcgtgccg ctgcccggcc tctacaacgc ctacaacgcg ctcgcagcca
      601 tcgcggtgtc gcgcgcgctg gacatcgcgc tgccgaccgc gacgcgcgcg atcggctcct
      661 tcaagcccgc cttcggccgc ctcgagcaga tctccgtcga cggacgctcg gtgcgccttg
      721 tgctcgtgaa gaaccccgcc ggcttcaacg ccgcgatcgg cgcgctcctc gagaccggac
      781 gcgagccgcg catcctcgcc gcgctgaacg atcgcgacgc cgacggccgc gacgtctcgt
      841 ggatctggga cgccgacttc gaatcgctcg cgccgcgcgt cgagcacgcg gtcgtgacgg
      901 gcatccgctc gcgcgatctc gcgaaccgtt tgaagtacgc cggcgtgccg cgtgagcggc
      961 tcgaggccgt ggacgacact gccagcgcga tcgatcgggc catcggcgcg accccgcgcg
     1021 aggaagagct catcgtgctc gcgacctata cggcgatgct tgcgatccgc gccgagctcg
     1081 cgcgccgcgg ccacgtcgcg ccgttctggg aggattgacg gatgccgggg atgccgcgct
     1141 tcaccaagac cccgcccgat gtcctcgcgg cgttcgaggc cgcgcgacca acgcggcgcg
     1201 acgtggatcg caggacgatg ttcggatacc cagcgctctt cgtgcgcggg aacatgttcg
     1261 cgttcacctt cggtccgaag gtcgcggtcc gcctcggcgc gtcggcgcgc gcccgtgccg
     1321 cgacggctgg cgcggcggcc ttcgaggtga tgccgggccg cgcgatgacc gagtacgtcg
     1381 ccgtcccgcc gagcgcgatg aagggcgcgg cgctgaagaa gtggctcgcc gacgcgctcg
     1441 cgtacgccga cacgctgccg cccaagggcg cggcgaagac cctgaggaag acgacggtga
     1501 agacggcaac gaagaccacc gcgaagacgg cgaagcggac cgcggcgaag cgctgatgga
     1561 tctgcgcatc gcgcatctct atcccgacct gatgagcatc tacggcgatc gcggcaacgt
     1621 gctcgcgctc tgccagcgcg cgcggtggcg cggcatcgac gcggaggtgc gcgcgtactc
     1681 ggcgggcacg tccttcgatg ccgactggcc ggacctgctc ttcttcggcg gcggtcagga
     1741 ccaggggcag gacatcgtcg gcgccgatct gcaaggtccc aacggcgacg cggtgcggcg
     1801 cgcgatcgcg ggcggcgccg cggcgctcac gatctgcggc ggctaccagc tgttcggcgt
     1861 cgagtacgtg cccgagaacg catcggcgat ccccggcctc ggtgtcctcg acgtgcgcac
     1921 cagggcgggg aagcggcgtt tcgtcgggaa tctcgtcgtc gagacgccgg acggcacgct
     1981 cgtcgggttc gagaaccaca gcggccgcac ctatctcggt ccgaaggcgc tcccgctcgg
     2041 cgcggtgctc gtcggccacg gcaacaacgg cgaggaccgc accgagggcg
//
LOCUS       DASTZR010000046         3096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_159623, whole genome shotgun sequence.
ACCESSION   DASTZR010000046 DASTZR010000000
VERSION     DASTZR010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159623"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="VFV20_01030"
     CDS             complement(<1..1178)
                     /locus_tag="VFV20_01030"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5286962.1"
                     /translation="MRELVGDYAPRGTRLLLLSMFLSTVPGGYLLVVLPLYLARAGLD
                     PAVIGGLYSASGAMTAVLVAFSGVLADRFGRRRFLVAGTVLPLGSYLIFAVTTDVPWL
                     VLASALGGVGLANGAAGALTIASFDALLADHTTAEGRTRVFALSQALWSLALALGSLC
                     AGVPELLAGTFAGLGTLEAYRPPYLVMVVLAVLATVALIPVQDDPEVHRARAASGWLP
                     RRSRDAILVYSVGIGFLGFGLGVAVQLLPLWFGLRFGVSEADLGPWYAAGQLASLATV
                     VAVPYCERRLGGPNTVLAALTGSAICLALIVVSPIFVVAAALHVVRSFLTNMSWPFHQ
                     SLLMSATVPEERGTAVGVGFAVWGTTNALGPLAAGVLLGGGIYALPLFVGAAMYVCGG
                     L"
     gene            1382..2914
                     /gene="rny"
                     /locus_tag="VFV20_01035"
     CDS             1382..2914
                     /gene="rny"
                     /locus_tag="VFV20_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005550497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEU5286963.1"
                     /translation="MAEPLVVGLVALVAGLLGFGIALFLRRSVALSSETTARANAERL
                     IAEARAKQKEIILEAKDEALKVAKTAETENRERRAELQRYETRLDKKDEQLDQKIAQV
                     DERDRRLGVKEKELDAERDKIAQLQEEQRTELARVASLSMEEARNLLLERVEDEMRDV
                     ISRRVREMEIDARERADAKARDIITMALQRYAAEHTTEHTVSVVALPSDDMKGRIIGR
                     EGRNIRTLETLTGVDLIIDDTPEAVVVSGFDPIRREVAKRALERLLVDGRIHPARIEE
                     MVAKARGEIDQIMKEAAESAVYEVGIAGLHPDLVKLLGRLHFRTSYGQNVLRHSIEVA
                     HVAGMLAAETGYDVQTMKRAGLLHDIGKAIDHEVEGAHTVIGGELLRRYGFPQTVIDG
                     VVGHHGDVEPVTMTGTLISAADAISAARPGARSEIVSNYIQRLQELESVANSFEGVEK
                     SFAIQAGREVRVIVKPEQVDDLTTARLSRDIAQKIQETMQYPGQVKVTVIRETRSVEY
                     AK"
BASE COUNT          580 a         1054 c         1079 g          383 t
ORIGIN      
        1 aggccgccgc agacgtacat cgccgcgccg acgaagagcg gcagcgcgta gatgccgccc
       61 ccaaggagga cgcccgcggc gagcggaccc agcgcgttcg tcgtgcccca caccgcgaag
      121 ccgacgccga cggccgtgcc gcgctcctcc ggcacggtcg cgctcatgag gagtgattga
      181 tggaagggcc aggacatgtt cgtgaggaag ctgcgcacga cgtgcagtgc cgcagcgacg
      241 acgaagatcg gcgagaccac gatgagcgca agacagatcg cggagccggt gagggcggca
      301 aggaccgtgt tgggtccgcc gaggcggcgc tcgcagtagg ggaccgccac cacggtcgcg
      361 agcgatgcga gctgcccggc ggcataccac ggtccgaggt cggcctcgct cacgccaaag
      421 cgcaggccga accagagggg aagcagctgc acggcgacgc cgaggccgaa ccccaggaat
      481 ccgatgccga cggagtacac caggatcgcg tcacgcgaac gcctcggcag ccagcccgat
      541 gcggcacgcg cgcggtgcac ctcgggatcg tcctggaccg ggatcaatgc gaccgtcgcg
      601 agcaccgcga gcacgaccat gacgagatac gggggacggt acgcctcgag cgtccccagc
      661 cccgcgaacg ttcccgccag gagctcgggc acgccggcac acaacgatcc gagggcgagg
      721 gccaggctcc agagcgcctg gctgagcgcg aacacacgcg tgcgaccctc cgcggtggtg
      781 tgatcggcga gcagggcgtc gaacgacgcg atggtgagcg ctcccgccgc gccgttggcg
      841 agacccacgc caccgagcgc gctcgccagc acgagccacg ggacgtcggt cgtcaccgcg
      901 aagatcaggt agctccccag cggcaggacg gttccggcca cgaggaagcg ccggcgtccg
      961 aaacgatcgg cgaggactcc cgagaacgcg acgagcacgg ccgtcatcgc gcccgacgcg
     1021 ctgtacagac cgccgatgac cgcggggtcg agcccggcgc gcgcgaggta gagcgggagg
     1081 accacgagca ggtagccgcc ggggacggtg ctgaggaaca tggagaggag caggagccgc
     1141 gtgccgcggg gcgcatagtc gccgacgagc tcacgcatcg agaaaaacga gcgtacttcg
     1201 cacgcttctt tgttcgcatt gcgtacgaca cgcgaccggc gctatcctcg cctcactaag
     1261 actttgtgaa ccctccggcc gaacggagca cgccgttcca tcggccggtt tgcgaaccgg
     1321 gggcactcgc cccgacacgg cgggcgtcct cgttcgcgct tacacggaaa ggaatagcga
     1381 catggccgaa cccctcgtcg tgggcctggt ggccctcgtc gcaggcttgc tcggcttcgg
     1441 gatcgctctc ttcctgcgtc gttcggtcgc gctctccagc gagaccaccg ctcgtgccaa
     1501 tgccgagcgg ctgatcgcgg aggcgcgcgc gaagcagaag gagatcatcc tcgaggccaa
     1561 ggacgaagcg ctgaaggtcg cgaagacagc cgaaactgag aaccgggaaa gacgcgccga
     1621 gctgcagcgg tacgagaccc ggctggacaa gaaggacgag cagctcgacc agaagatcgc
     1681 tcaggtcgac gaacgcgacc gccggctcgg cgtgaaagag aaagagctgg acgccgagcg
     1741 ggacaagatc gcacagctcc aggaggagca gcggacggag ctcgcccggg tcgcttctct
     1801 gtccatggag gaggcgcgca acctgctcct cgagcgcgtc gaagacgaga tgcgagatgt
     1861 catcagccgc cgcgtccgcg agatggagat cgacgcgcgc gagcgcgccg acgccaaggc
     1921 ccgcgacatc atcaccatgg cgctccagcg gtacgccgcc gagcacacca ccgagcacac
     1981 cgtgagcgtg gtcgcgctgc cgagcgacga catgaagggc cgcatcatcg gccgtgaggg
     2041 gcgcaacatc cgcaccctcg agacgctcac cggcgtcgac ctcatcatcg acgacacgcc
     2101 cgaggcggtc gtcgtgtccg gcttcgaccc gatccgccgc gaggtcgcga agcgcgcgct
     2161 cgagcggctc ctcgtcgacg gtcgcatcca tccggcccgg atcgaagaga tggtggccaa
     2221 ggcccgcggc gagatcgacc agatcatgaa ggaggcggcc gagtccgccg tgtacgaggt
     2281 cggcatcgcc ggcctgcatc cggatctcgt gaagctcctc ggacgcctgc acttccgcac
     2341 ctcctacggg cagaacgtcc tgcgccactc gatcgaggtc gcgcacgtcg cgggcatgct
     2401 cgcggcggag accgggtacg acgtccagac catgaagcgc gccggcctgc tgcacgacat
     2461 cggcaaggcg atcgaccacg aggtggaggg tgcgcacacc gtcatcggtg gcgagctgct
     2521 gcgccgctat ggcttcccgc agacggtgat cgacggcgtc gtcggccacc acggggatgt
     2581 cgagcccgtc acgatgaccg gaacgctcat ctcggcggcc gacgcgatca gcgcggcacg
     2641 ccccggcgca cgcagcgaga tcgtgtcgaa ctacatccag cgcctgcagg agctcgagag
     2701 cgtcgcgaac agcttcgagg gcgtcgagaa gtccttcgcc atccaggcgg gacgcgaggt
     2761 gcgcgtgatc gtgaagccgg agcaggtgga cgacctcacg acggcccgcc tctcccgcga
     2821 catcgcgcag aagatccagg agacgatgca gtacccggga caggtcaaag tgacggtgat
     2881 ccgcgagacg cgctccgtgg agtacgcgaa gtaatagtgg tggcgtcggt cggagcgcgc
     2941 ggcgacgacc gaaaccctct cttcgcgggg tatcccgtcg ctcgtcgcga cgggaaccct
     3001 ccccgcgcgg gggcgcctcg gtcgccgctc tcgcctacac ccgttcctta gccgttcctt
     3061 ggtcgcgccc cgcgtcggtt gctgttggcg ctcggt
//
LOCUS       DASTZR010000047         3003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1152082, whole genome shotgun sequence.
ACCESSION   DASTZR010000047 DASTZR010000000
VERSION     DASTZR010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152082"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VFV20_01040"
     CDS             <1..372
                     /locus_tag="VFV20_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5286964.1"
                     /translation="GIANYSRILRTETLDVLGQDFVRTAHAKGLRERTVMFVHALRNA
                     LIPVVTALGGILAGLVGGALVIEQVFSWPGIGRFTFAAAIAKDYPVIQAGVMISALLL
                     VISYILRDIVYAVVDPRISVK"
     gene            372..1304
                     /locus_tag="VFV20_01045"
     CDS             372..1304
                     /locus_tag="VFV20_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5286965.1"
                     /translation="MAATTTAVTAVSGNDILVRPARSLWSNALWKLRHDRLTIVATVV
                     LVLLAVSSAAADLFSQYVFQYSFEKQDLLHTYQRPTAEPLAYLLGTDELGRSAVVRLL
                     YGGRVSLSVGFVAALVNLTIGIALGVSAGYFRGWVDDIFTWFVTTLQSIPTIFLLLII
                     AAIWEPGPVTLTVIIGALFWTGISLYCRGQTFALREREFVVAARTIGARDRRIMFNHI
                     LPNILPLIFVLAAIDIGGIILAESALSFLGLGILPPTPTWGNMLTGAVGNLVRGPWLI
                     YPPGAMIFLTVLCLYLIGDGLRDALDPRIRGTAG"
     gene            1358..2872
                     /locus_tag="VFV20_01050"
     CDS             1358..2872
                     /locus_tag="VFV20_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5286966.1"
                     /translation="MLAIACTSVPAATSATPTATQDLVRGGTLRVALTTDITSLDPWT
                     ASDADTFAVLRQMYESLVAFEPGTLRIVPRLAFRWTVSPDARTWTFTLRSGVRFHDGT
                     PLDAAAVAFNFERARAFARFDLGSLAESVTAPDPSTVVFALRAPYAGLLATLASPSFA
                     IVSPSCVRQGPAWATSGSRCGAGTGPFKLEAGGWRPGERVTLTRNAAYWGTDASGRRL
                     PLLDGVVFTPVRDETVRVGGLKAAAVDLVLDLAPASIRTLRADPNIAAASSPMYATLF
                     LGFGNARPFDSLEVRRAVALTVDRGSVVQAAYAGQARPAVQLLPPGVLGYDDTVTQFA
                     PMDVSAAKKALADASLSAGFATELWYSPDATTTLPDPKRVAESIASDLVKIGLTVTVR
                     TEDPAAFAADARAGRLPLWIGMREPERPDPDEFLADASTDPVVLELLRGARGEPDASK
                     RGEVYKQVTKLVQQSVSRIPLLHTGGLAGLSKKVQGFVPAAVGPEALETVFFRN"
BASE COUNT          372 a         1130 c          987 g          514 t
ORIGIN      
        1 ggcatcgcga actactcgcg catcctccgc accgagacgc tcgacgtgct gggccaggac
       61 ttcgttcgca ccgcgcacgc caagggcctg cgtgaacgaa cggtgatgtt cgtccacgcg
      121 ctccgaaacg cgctcatccc cgtggtcacc gcgctgggcg gcatcctggc cggtctcgtc
      181 ggcggcgcgc tggtcatcga gcaggtcttc agctggcccg gcatcggccg cttcacgttc
      241 gccgcggcga tcgccaagga ctatccggtc atccaggcgg gtgtcatgat ctccgccttg
      301 ctgctcgtca tcagctacat cctccgcgac atcgtctacg cggtcgtcga tccgcgtatc
      361 tcggtgaagt gatggcagcc accaccacgg cggtcaccgc cgtcagcggg aacgacatcc
      421 tcgtccggcc cgcgcgcagc ctgtggtcca acgccctgtg gaagctccgc cacgaccggc
      481 tgacgatcgt ggcgaccgtc gtgctcgtcc tgctggccgt gtcctcggcc gccgccgacc
      541 tgttctcgca gtacgtgttc cagtacagct tcgagaagca ggacctcctc cacacctatc
      601 agcggcccac tgccgaaccg ctcgcgtacc tactcgggac cgacgagctc ggacgcagcg
      661 cggtggtgcg tctgctctac ggaggccgcg tctcgctctc ggtcggattc gtcgccgccc
      721 tcgtcaatct cacgatcggc atcgcgctcg gcgtgtcggc gggctacttc cgcggctggg
      781 tcgacgacat cttcacctgg ttcgtgacga cgctgcagtc gatcccgacg atcttcctgc
      841 tgctcatcat cgcggccatc tgggagccgg gcccggtgac gctgacggtg atcatcggcg
      901 cgctcttctg gaccggcatc tcgctgtact gccgcggcca gacgttcgcg ctccgcgagc
      961 gggagttcgt ggtcgcggcg cgcaccatcg gcgcgcgcga tcgccgcatc atgttcaacc
     1021 acatccttcc gaacatcctg ccgctcatct tcgtcctcgc ggccatcgac atcggcggca
     1081 tcatcctcgc cgagtccgcg ctctcgttcc tcggcctcgg gatccttccg cccacgccca
     1141 cgtgggggaa catgctcacc ggcgccgtcg gcaacctggt gcgcgggcca tggctcattt
     1201 acccgcccgg cgcgatgatc ttcctcacgg tcctgtgtct ctacctcatc ggcgatggcc
     1261 tgcgcgatgc gctcgacccg cgtatccgcg gcaccgcggg ctagcgcagc tcgggctgta
     1321 ccggtcctct cgcgggcgtc tcgtcgcgct cgcgctggtg ctggcgatcg cgtgtacatc
     1381 cgtacccgcc gcgacatccg cgaccccgac cgcgacgcag gatctggtgc gcgggggcac
     1441 gctgcgcgtc gcgctcacga ccgacatcac ctcgctcgat ccctggaccg cgtccgacgc
     1501 ggacacgttc gcggtgctgc gtcagatgta cgagtccctc gtcgcgttcg agcccggcac
     1561 gctgcgcatc gtcccccggc tggccttccg ctggacggtc tcgcccgacg cccgcacgtg
     1621 gaccttcacc ctgcgcagcg gcgtgcggtt ccacgacggc acgccgctcg atgccgccgc
     1681 ggtcgcgttc aacttcgaac gcgcgcgcgc cttcgcgcgc ttcgacctcg gttcgctcgc
     1741 tgagtcggtg accgcgccgg acccgtccac ggtggtcttc gcgctgcgcg ctccgtacgc
     1801 cggcctgctc gcgacgctcg cgtcgccgtc cttcgcgatc gtgagcccgt cgtgcgtccg
     1861 gcagggaccg gcctgggcga cgtcgggctc gcgctgtggg gccggcacgg gcccgttcaa
     1921 gctcgaggcc ggggggtgga ggcccggcga gcgcgtgacg ctgacgcgga atgccgcgta
     1981 ctggggcacc gatgcgagcg gccgtcggct gccgctcctc gacggcgtcg tgttcactcc
     2041 ggtgcgcgac gagaccgtgc gcgtcggcgg cctcaaggcg gcggcggtgg atctcgtgct
     2101 cgacctcgcg ccggcgagca tccgtacgct gcgcgccgac ccgaacatcg ccgcggcgtc
     2161 cagtcccatg tacgcgacgc tcttcctcgg gttcggcaac gcacggccat tcgactcgct
     2221 cgaggtgcga cgcgccgtgg ccctcaccgt ggaccgcggc tccgtcgtgc aggcggcgta
     2281 tgcgggacag gcgcggcccg ccgtgcagct gctcccgccc ggcgtgctcg gctacgacga
     2341 caccgtgacg cagttcgcgc cgatggacgt ctcggcggcg aagaaggccc tcgcggacgc
     2401 gtccctctcc gcgggcttcg cgaccgagct ctggtattca cccgacgcga cgacgacgct
     2461 gccggatccg aagcgcgtgg ccgagtcgat cgcctcggat ctggtgaaga tcggcctgac
     2521 cgtgaccgtc cggacggagg atccggccgc gttcgccgcc gacgcgcgtg cgggccggct
     2581 gcccctctgg atcggcatgc gcgagcccga gcgccccgat cccgacgagt tcctcgccga
     2641 cgcgtcgacg gacccagtcg ttctcgagct gctccgcggg gcacgaggcg agcccgacgc
     2701 ctcgaagcgc ggcgaggttt acaagcaggt cacgaagctg gtgcagcaaa gcgtgtcgcg
     2761 gatcccgctc ctccacaccg gtggcctcgc cgggctctcg aagaaggtcc agggattcgt
     2821 gcccgccgcc gtcggccccg aggccctgga gaccgtcttc ttccgcaact gacgctcgtt
     2881 tcgtcggtgc ggcggccggc gcgagcgcgc ggggcaggct cgtctcgctc gaatcactcc
     2941 cgccagcact tagcgctcgg ccgcgcgacg ctcctgagct ggcggtcgtg ggtctcgctc
     3001 gac
//
LOCUS       DASTZR010000048         3965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1126041, whole genome shotgun sequence.
ACCESSION   DASTZR010000048 DASTZR010000000
VERSION     DASTZR010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126041"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1145)
                     /locus_tag="VFV20_01055"
     CDS             complement(105..1145)
                     /locus_tag="VFV20_01055"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5286967.1"
                     /translation="MIRALVLAFVVLATACAPSGLGAPATSAPAASPSAYPVEQVTFM
                     AGFKPQADLPFVGVYVAQEKGYFKAQGLEVTIQHATTGEHVQLLATDRIQFSTGTGGD
                     ILKRVAQAGVPLVSVAVIGQRDEQAFAVKADSPIATVKDWEGRLVGYKSTPSADYLAM
                     LKIAGVDRSKVREVAVGFDPRVLATGQVDVYPVFAANEPDTLARQGIAVRLFDPTAFG
                     IPSFGLTYMTNQATASKRPDVVRRFLRAALKGIEDAVADRDGAIAIVMKYAPGEDRAH
                     QRYMMDTEIAAAQNERTRAEGIGTMTREQWAALESFLAEYGTLPKRVDVATVFNDTFV
                     NELYRDGKLIWR"
     gene            complement(1142..1885)
                     /locus_tag="VFV20_01060"
     CDS             complement(1142..1885)
                     /locus_tag="VFV20_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5286968.1"
                     /translation="MLLASAAIVVVAIALEIWTAALRVPGYLVPAPSAVAARFFADWP
                     TFLREASVTIGEAAMGFVVGTAVAFAIASAMVRSRILERTLFPLAILVKLTPIVAIAP
                     LFTLWFGFGSAPKVAIAALITFFPMVVNAFVGLRSVDPQEMAFLETLGASRGEIFRLL
                     RVPTALPYLFSGARISLNLALIGAVIGEWTGADRGLGRVILVANANLDLATLFAAVLL
                     LAAIGIAANALVGVAERRVLHWHPVELGA"
     gene            complement(1912..2652)
                     /locus_tag="VFV20_01065"
     CDS             complement(1912..2652)
                     /locus_tag="VFV20_01065"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5286969.1"
                     /translation="MGVAIEIAGASAGYDGAPVLLGIDLAVGGGEVLAVVGRSGSGKT
                     TLLRLIAGTLAPSAGTVRLLGEHPDVARRAKRIGFVGQNAALHPWRTVLQNVRLPLEV
                     NPADVEDGPTPDEWVARIGLEAAARAYPHQLSGGMRQRVALARSLVADPAILLMDEPL
                     SSLDELTRDDLRLELVDFWGEKRTVVYVTHDIEEAVWLADRVITLGGQPARITGTVPV
                     PLPRPRTPALRRDARFMDLVDAVRGSLA"
     gene            2770..3159
                     /locus_tag="VFV20_01070"
     CDS             2770..3159
                     /locus_tag="VFV20_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286970.1"
                     /translation="MAVAVVVAGCLAFAGLVSTGRLQPVTVDDAPAVTAEHASLPVVP
                     LGVVPQTLQLPPGLGSVTLSSMPDRLANEAAPATLRAVVVVRGTRGVASVDGPSQIYW
                     TEDGVAYRLIASDRTIDELVRIAETLR"
     gene            3159..3698
                     /locus_tag="VFV20_01075"
     CDS             3159..3698
                     /locus_tag="VFV20_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286971.1"
                     /translation="MPRPLEVLLALAATALDAAFFASTPYWPKEPQRPLHVPVAAFAA
                     AAPQDQLPSRKAPLLGPSVLAGVRPAPELSVALPDDLAVVVQRRGIDGETGLIPSAAS
                     ITTFRLRASGDLVSVASSLGVDPVRGDAALTVRGAAAIVSTDRGITTIRWTERGATYE
                     ISSRTLDATRLAAVADQLR"
     gene            complement(3748..>3965)
                     /locus_tag="VFV20_01080"
     CDS             complement(3748..>3965)
                     /locus_tag="VFV20_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286972.1"
                     /translation="LPQRDAVQPAFDVPIVLRRPTESLLESIVEGVESGVTVEQRRDE
                     GAVDVRESIAVQHGPAFAVRRSVHMLE"
BASE COUNT          578 a         1423 c         1401 g          563 t
ORIGIN      
        1 ccgcaggccg ccatgatgaa cgcgaccgcc accgtgagct tcgagaaccg acccatctgc
       61 atccctccgt cgtggtcgta ggagggacat tgtgcgcccg ccggctagcg ccagatgagc
      121 tttccgtccc ggtagagctc gttcacgaat gtgtcgttga agacggtcgc gacgtcgacg
      181 cgcttaggca gcgtcccgta ctcggcgagg aacgactcga gcgcggccca ctgctcgcgc
      241 gtcatcgtgc cgatgccttc cgcgcgcgtg cgctcgttct gcgcggcggc gatctcggta
      301 tccatcatgt agcgctggtg cgcgcggtcc tcgccgggcg cgtacttcat cacgatcgcg
      361 atggcgccgt cgcggtcggc cacggcgtcc tcgatgccct tcagcgcggc gcgcaggaag
      421 cggcggacga cgtcgggacg cttcgacgcg gtggcctggt tcgtcatgta ggtgaggccg
      481 aaggagggga tgccgaaggc cgtcggatcg aacagccgca ccgcgatgcc ctgccgggcc
      541 agcgtgtcgg gctcgttcgc cgcgaagacc ggatacacgt ccacctggcc ggtcgccagc
      601 acgcgcggat cgaacccgac cgcaacctcg cgcaccttgg agcgatccac gcccgcgatc
      661 ttcagcatcg cgaggtagtc cgcggacgga gtcgacttgt agccgacgag cctgccctcc
      721 cagtccttga ccgtcgcgat gggggagtcg gccttcaccg cgaacgcctg ctcgtcgcgc
      781 tggccgatca ccgccaccga gacgagcggc acgccggcct gcgcgacgcg cttcaggatg
      841 tcgccgccgg taccggtgga gaactggatc cggtcggtcg cgaggagctg cacgtgctcg
      901 ccggtggtgg cgtgctggat ggtgacctcg aggccctgcg ctttgaagta ccccttctcc
      961 tgcgcgacgt acacgccgac gaagggaaga tccgcctgcg gcttgaagcc ggccatgaac
     1021 gtgacctgct ccaccggata ggcgctcggc gacgccgccg gcgcgctcgt cgcgggcgcg
     1081 cccagcccag agggcgcgca ggccgtcgcg aggacgacga acgcgaggac gagcgcgcgg
     1141 atcatgcgcc gagttccacg ggatgccagt gaagcacgcg gcgctcggcg acgccaacga
     1201 gcgcgttcgc ggcgatgccg atcgcggcga gcagcagcac cgccgcgaag agcgtcgcca
     1261 ggtcgaggtt cgcgttggcg acgaggatga cgcgtccgag cccgcggtcg gcgcccgtcc
     1321 actcaccgat gaccgcgccg atgagcgcga ggttgaggct gatgcgcgcg ccggagaaga
     1381 ggtacggcag cgccgtgggg acgcgcagga gccggaagat ctcaccccgc gacgcgccga
     1441 gcgtctcgag gaacgccatc tcctgcggat cgaccgaccg cagtccgacg aatgcgttca
     1501 cgaccatcgg gaagaacgtg atgagcgccg cgatcgcgac cttcggcgcg ctgccgaagc
     1561 cgaaccagag cgtgaagagc ggagctatcg ccacgatcgg tgtgagcttc acgaggatcg
     1621 cgagggggaa gagcgtccgt tccaggatcc gcgagcgcac catcgcgctc gcgatcgcga
     1681 aggcgaccgc cgtcccgacg acgaagccca tcgcggcctc cccgatcgtc accgacgcct
     1741 cgcgcaggaa cgtcggccag tccgcgaaga atcgggccgc gaccgcgctc ggcgcgggca
     1801 cgagatagcc gggtacgcgc agcgccgcgg tccagatctc gagcgcgatc gcgacgacga
     1861 cgatcgccgc gctcgcgagg agcacgtcca ggagggtgcg tcgtgcactg ttcacgcgag
     1921 cgacccgcgg acggcatcga cgaggtccat gaaccgcgcg tcgcggcgga gcgccggcgt
     1981 gcgcgggcgt ggcagcggga cgggcacggt cccggtgatg cgtgcgggct ggccgccgag
     2041 cgtgatcacg cgatcggcca gccacaccgc ctcctcgatg tcgtgcgtca cgtagacgac
     2101 cgtgcgcttc tcgccccaga agtcgaccag ctcgaggcgc aggtcgtcgc gggtcagctc
     2161 gtcgagcgac gagagtggct cgtccatcag caggatcgcc gggtcggcga cgagcgatcg
     2221 cgcgagcgcg acgcgctggc gcatcccgcc ggagagctgg tgcgggtagg cccgcgcggc
     2281 cgcctcgagg ccgatgcgcg cgacccactc gtcgggcgtc ggcccatcct cgacgtccgc
     2341 gggattcacc tccaggggca ggcggacgtt ctgcaggacc gtgcgccacg gatgcagcgc
     2401 cgcgttctgc ccgacgaagc cgatccgctt cgcgcgccgc gcgacatcgg gatgctcgcc
     2461 gaggaggcgc acggtgcccg cgctcggcgc gagcgtcccg gcgatcaggc gaaggagcgt
     2521 cgtcttcccg ctcccgctgc ggccgacgac cgccagcacc tcgccgcccc cgaccgccag
     2581 gtcgatgccc aggagcacgg gcgcgccgtc atagcccgcg ctcgcaccgg cgatctcgat
     2641 cgcaacgccc atcgaacgga tgctaggtcg ccgcctgcct ctagcattcc gacgaacgcg
     2701 atgcgcggtg tgatcgtcga cctcgatcga tcgtcgcggt cggagccaag gacgcgccct
     2761 gcggatctct tggccgtcgc cgtggtggtc gccggatgtc ttgcgttcgc ggggctcgtc
     2821 tcgaccgggc gcctgcagcc cgtcaccgtc gacgacgcac cggcggtgac ggcggaacac
     2881 gcgagcctgc ccgtggtgcc gcttggtgtc gtgccgcaga cgctgcagct tccacccgga
     2941 ctcgggagcg tcacgctgtc gtcgatgccg gaccgcctgg cgaacgaggc ggcacccgcg
     3001 acgctccgtg ccgtggtcgt cgtccgcggc acgcgcggcg tcgcgtccgt ggacggtccg
     3061 tcgcagatct actggaccga ggacggcgtc gcctaccggc tcatcgcctc cgaccgcacg
     3121 atcgacgagc tggtccgcat cgccgagacg ctgcgctgat gccgcgcccg ctggaggtgc
     3181 tgctcgcgct cgcggcgacc gcgctcgacg ccgcgttctt cgcgagcact ccgtattggc
     3241 cgaaagagcc gcagcggccg ctgcacgtcc cggtcgccgc tttcgcggcc gcggcgccgc
     3301 aggatcagct gccgtcgcga aaggctccgc tcctcgggcc ctcggtcctc gcgggcgtac
     3361 ggccggcgcc ggagctgagc gtcgcgcttc ccgacgatct cgccgtcgtc gtccagcgcc
     3421 gcggcatcga cggggagacg gggctcatcc ccagcgccgc gtcgatcacg accttccggc
     3481 tccgcgcgag cggcgacctc gtctccgtcg cgtcctcgct cggcgtcgat cccgtgcgcg
     3541 gcgacgcggc gctcacggtg cgtggtgccg cggccatcgt ctccaccgac cgtggcatca
     3601 cgaccatccg ctggaccgag cgcggcgcga cctacgagat ctcttcgcga acgctcgacg
     3661 cgacgcgtct ggcggcggtg gcggatcagc tccgctagcc gtttcggcac ggggcaggca
     3721 gcggctaaag gaatcatggg cgacaggtca ttccagcatg tggaccgatc gccggaccgc
     3781 gaacgctggt ccgtgctgta ccgcgatgct ttcccgcacg tctaccgcgc cctcgtcgcg
     3841 gcgctgttcg accgtgacgc cgctctcgac gccctccacg atgctttcga ggagggactc
     3901 cgtcggccgc cgcaggacga tcggaacgtc gaaggctggc tgtaccgcgt cgcgctgcgg
     3961 aaggc
//
LOCUS       DASTZR010000049         3808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1230357, whole genome shotgun sequence.
ACCESSION   DASTZR010000049 DASTZR010000000
VERSION     DASTZR010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230357"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /gene="rsmH"
                     /locus_tag="VFV20_01085"
     CDS             complement(<1..665)
                     /gene="rsmH"
                     /locus_tag="VFV20_01085"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU5286973.1"
                     /translation="MAEVLAQLDPREAGRFLDGTVGGGGHAEAILERTAPTGRLLGLD
                     VDPKALAEARRVLARFGDRAVLVRSNFALCDVVAREHGFDALDGVLLDLGLSSIQLAD
                     DTRGFSFRASGPLDMRADPELELTAGDIVNAWDERELRRVFADYGEEPEAARIAAAIV
                     RRRTREPFLTADDLGRFVFGVKRQRSRATDPATRVFQALRIAVNDELANLERGLAAAL
                     SLLR"
     gene            complement(694..1125)
                     /gene="mraZ"
                     /locus_tag="VFV20_01090"
     CDS             complement(694..1125)
                     /gene="mraZ"
                     /locus_tag="VFV20_01090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEU5286974.1"
                     /translation="MEGYFAGEFAHSLDEKGRLAIPAKFRGRFKEGAVVTRWTGECLA
                     VWPAPEWTALNADIARRPRTDPAATRFRHFLLAGAHEGDPDAQGRLTLPTHLRDFAHL
                     KNEAVVIGNSDHLEVWEPGRWRANLASVQTAIGDDLKDLGV"
     gene            complement(1263..1943)
                     /locus_tag="VFV20_01095"
     CDS             complement(1263..1943)
                     /locus_tag="VFV20_01095"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HEU5286975.1"
                     /translation="MTRPRRIVPCGGQQLFHPALTRYVFGLARRPRPRVLFLPTASGD
                     SPDYLLTFYRQLAGVDCEPRHLELYHRSVDDIGALLSEQDVVMVGGGNTANMLAIWRL
                     HGVDRALREAYAGGTILTGWSAGCLCWYEGGITDSFTPELGPLRDGLGLLTGTACPHY
                     DSEARRPLVYGREIAAGLPAGIALDDGVAALYEDEQLAEIVSARPDGRAFRVDAKGDH
                     PLPVRHIA"
     gene            complement(1956..3029)
                     /locus_tag="VFV20_01100"
     CDS             complement(1956..3029)
                     /locus_tag="VFV20_01100"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEU5286976.1"
                     /translation="MAKNAVITGWGFYAPARVMTNMDLERIVDTSDEWIASRTGIRER
                     RIAGDAENTSSMATRAGRAALERAKLRPQDVQLIIVGTCSPDYLFPATACIVQSELGA
                     VRAGAFDVEAACTSFVTALGIARGMIMSGTVQNALVIGAETLSRLLNWKDRSTCVLFG
                     DGAGAVVVEASNASVGIESVVLHSEGSKGDLLMVPAGATRMPASKETLEQGKHFITMQ
                     GGEVFKLAVKSMSDAAEEALREASLTLDDIDLMIPHQANLRIIEGVAKRLHFPMDKVF
                     VNIQRYGNTSAASVPIAIAEAAATGRMKRGDKILLVAFGGGFTWGASVLEWFGPHDGV
                     RPPSVLDRARETLEDVVDRVRPV"
     gene            complement(3123..3494)
                     /locus_tag="VFV20_01105"
     CDS             complement(3123..3494)
                     /locus_tag="VFV20_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286977.1"
                     /translation="MNILRFLREQAKNYDCNVCGTNHSRSEISVLGKREGGWVVRVTC
                     SKCETVVTLLVFVGDKAQETTATSAAKKTAPTAAPRRPRRPPVTLDEVLDAHELLDSY
                     TGDAQGLFAPRGKVRTSTDAS"
     gene            complement(3498..>3808)
                     /locus_tag="VFV20_01110"
     CDS             complement(3498..>3808)
                     /locus_tag="VFV20_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018655535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU5286978.1"
                     /translation="QYRWLRAYSPYHHVREGVRYPAVLFTTAEGDSRVDPMHARKMTA
                     LMQATTAEDPDAVVLLRVERDAGHGVGKPLDKQVDDLADQYSFFAWRLGSESRSGYAR
                     "
BASE COUNT          566 a         1316 c         1347 g          579 t
ORIGIN      
        1 cgcaggagcg acagcgcggc cgccaggcca cgctcgaggt tcgcgagctc gtcgttcacc
       61 gcgatgcgga gcgcctggaa gacgcgcgtc gcgggatcgg tggcacgaga gcgctgccgc
      121 ttcacgccga acacgaaacg accgaggtcg tcagcggtga ggaacggctc gcgtgtgcgg
      181 cggcgcacga tggccgcggc gatgcgcgcg gcctccggct cctcgccgta gtcggcgaag
      241 acgcgccgca gctcgcgctc gtcccaggcg ttcacgatgt cgcccgccgt gagctcgagc
      301 tcgggatcgg cgcgcatgtc cagtggcccg ctcgcgcgga agctgaaacc gcgggtgtca
      361 tcggcgagct ggatcgacga gagcccgaga tcgaggagga cgccgtcgag cgcgtcgaag
      421 ccgtgttcgc gggccacgac gtcgcaaagc gcgaagttgg atcgaacgag gaccgcgcga
      481 tcgccgaagc gtgcgaggac gcgccgtgcc tccgcgagcg ctttggggtc gacgtcgagc
      541 ccgagaagcc ggccggtcgg cgcggtgcgc tcgaggatgg cctctgcgtg gcctccccca
      601 cccaccgtgc cgtcgaggaa gcggccagct tcgcggggat caagttgggc gagaacttcg
      661 gccaggagaa ccggctcatg aagcctccca tctttagacc ccgaggtcct tgaggtcgtc
      721 gccgatggcg gtctggacgc tcgcgaggtt cgcgcgccag cgtcccggct cccagacctc
      781 gaggtggtcg gagttgccga tgacgacggc ttcgttcttg aggtgcgcga agtcgcggag
      841 gtgcgtcggg agcgtgagcc ttccctgcgc gtcggggtca ccctcgtggg ctccggcgag
      901 caggaagtgg cggaagcgcg tcgcggccgg atccgtccgg ggacgccgtg cgatgtcggc
      961 gttgagcgcg gtccattccg gcgcgggcca tacggcaagg cactcgcccg tccaacgcgt
     1021 cacgaccgcc ccttccttga agcggccgcg gaacttggcc gggatggcga ggcgcccttt
     1081 ctcgtccagc gagtgggcga attcgcccgc gaaatagcct tccaatcagc cttcccatcg
     1141 agaggagcgg ttccccaccg ttccccacat ttcaccacgc aggtggcatt ctccaccacc
     1201 acgcggcctt gtcaaggggg agttcaggga caaccccgcg tcgagctcgg cgggctggcc
     1261 ccctacgcga tgtgccggac gggtagcgga tggtctcctt tggcgtcgac gcggaaggcc
     1321 ctgccgtcgg ggcgggcgct cacgatctcc gccagctgct cgtcctcgta cagcgcggcc
     1381 acgccgtcgt ccagcgcgat gcccgcgggc agcccggcgg cgatctcgcg cccgtacacg
     1441 agcgggcggc gcgcctccga gtcgtagtgc gggcacgccg tccccgtgag gaggccgagc
     1501 ccgtcgcgca gcggcccgag ctcgggcgtg aacgaatcgg tgatcccgcc ctcgtaccag
     1561 cagaggcagc ccgcggacca gccggtgagg atcgtgccgc ccgcgtacgc ctcgcggagc
     1621 gcgcggtcga cgccgtgcag ccgccagatc gcgagcatgt tcgcggtgtt gccgccgccg
     1681 accatgacga cgtcctgctc ggagaggagc gcgccgatgt cgtcgacgga gcggtggtac
     1741 agctcgaggt ggcgcggctc gcagtcgacg ccggcgagct gccgatagaa ggtgagcaga
     1801 tagtcggggc tgtcgcccga cgcggtcggc aggaagagca cgcgcggccg cggccggcgc
     1861 gcgagcccaa agacgtagcg cgtgagcgcg ggatgaaaga gctgctgccc gccacagggg
     1921 acgatccgcc gaggacgtgt cacgccaggt ccgtgctaga cgggccggac ccgatcgacg
     1981 acgtcctcga gcgtctcgcg cgcgcgatcg aggacggacg gcggacgcac accgtcatgc
     2041 ggtccgaacc actcgaggac cgacgcgccc caggtgaagc cgccgccgaa ggcgacgagg
     2101 aggatcttgt cgccgcgctt catgcgcccg gtcgccgcgg cttcggcgat ggcgatcggc
     2161 acggacgccg ccgatgtgtt gccgtaccgc tggatgttca cgaagacttt gtccatcggg
     2221 aagtgcaggc gcttcgcgac gccctcgatg atccgcaggt tcgcctggtg tgggatcatc
     2281 agatcgatgt cgtcgagcgt gaggctcgcc tcgcgcagtg cttcctcggc ggcatcggac
     2341 atcgacttca ccgcgagctt gaagacctca cccccctgca tggtgatgaa gtgcttcccc
     2401 tgctcgagcg tctccttcga cgcgggcatg cgcgtcgcgc cggcggggac catgaggagg
     2461 tcgcccttgc tgccctcgct gtgcagcacg acggactcga tccccaccga ggcgttggac
     2521 gcctcgacga ccaccgcgcc cgcaccgtcg ccgaagagca cgcacgtgga acggtccttc
     2581 cagttcagca gccgcgagag ggtctcggcg ccgatgacga gcgcgttctg gaccgtgccg
     2641 gacatgatca tgccgcgcgc gatgccgagc gccgtcacga agctggtgca cgcggcctcg
     2701 acgtcgaacg cgccggcgcg caccgctccc agctccgact gcacgatgca cgcggtcgcg
     2761 gggaacaggt aatccggcga gcacgtgccg acgatgatga gctgcacgtc ctgcgggcgg
     2821 agcttcgcgc gctcgagcgc ggcgcggccc gcgcgcgtgg ccattgagga ggtgttctcg
     2881 gcatcgccgg cgatgcgtcg ctcgcggatg cccgtgcggc tcgcgatcca ttcgtcggag
     2941 gtgtcgacga tgcgctcgag gtccatgttg gtcatcacgc gcgccggcgc gtagaagccc
     3001 cagccggtga tgaccgcgtt cttggccacc tagctcaacc gctcgttgtc actcgcgagg
     3061 cggtctcgct cctcgcagga acctctcgtc gctcgtctca tgacattctc acgcgctcgc
     3121 gctcacgacg cgtcggtcga ggtgcggacc ttcccccgcg gcgcgaagag accctgcgcg
     3181 tcgccggtgt acgagtcgag cagctcatgc gcgtccagga cctcatcaag cgtgacgggc
     3241 ggccgccgcg ggcgacgcgg ggcggcggtc ggggcggtct tcttcgccgc actcgtcgcc
     3301 gtcgtctcct gcgccttgtc cccgacgaag accaggagcg tgaccaccgt ctcgcacttc
     3361 gagcacgtca cccgcacgac ccaaccgccc tcgcgcttgc cgagcacgct gatctcggag
     3421 cggctgtggt tcgtgccgca gacgttgcag tcgtagttct tcgcctgctc gcggaggaag
     3481 cggaggatgt tcaccttcta tcgagcataa ccggagcggg actccgatcc gaggcgccag
     3541 gcgaagaatg aatactggtc ggcgagatcg tcgacctgct tgtccagcgg ctttcccacc
     3601 ccgtgtccgg cgtcgcgctc gacgcgcagc aggacgaccg cgtcggggtc ctccgcggtc
     3661 gtcgcctgca tgagcgccgt catcttgcgc gcgtgcatcg ggtcgacacg gctatcgccc
     3721 tccgccgtcg tgaagaggac cgccggatag cgcaccccct cccggacgtg gtggtacggc
     3781 gagtacgcgc ggagccaccg gtactgcg
//
LOCUS       DASTZR010000050         2418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1178220, whole genome shotgun sequence.
ACCESSION   DASTZR010000050 DASTZR010000000
VERSION     DASTZR010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178220"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..944
                     /gene="mreC"
                     /locus_tag="VFV20_01115"
     CDS             102..944
                     /gene="mreC"
                     /locus_tag="VFV20_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU5286979.1"
                     /translation="MASLGRTRRSQNGFLRSFAVLFLISTLLLLARNWDPLRSLATAG
                     TELLVPVQRVLADAGITVNQFTQAISEIQRLRADNAKLHDDVDRLTLENVRLREAAFA
                     AQQAAKLQQAAATLSFETVRATVIARDPSNILATLVLGVGANDGVKAGHVVVSDQGLV
                     GRISEVGPSYSKVLLLTDPASSLSALVEGSRATGIVRGQYGDALIMEWILQTEAVKPG
                     DVVVTAGLGLGDELRSLYPKGLVVGKIVQLQNAEAAAYQRAIIVPAVDLRRLENVLVV
                     RTTP"
     gene            1065..>2418
                     /locus_tag="VFV20_01120"
     CDS             1065..>2418
                     /locus_tag="VFV20_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU5286980.1"
                     /translation="MPTETLHLLVQFAAGTSDADRTAIARDLGGTIEGEIRPLGVTRL
                     AITVERGTDPEAVLRAIAANRKVTTAEQDARVHLDGTPNDQYWDTDPFVGLGQWGAKK
                     VMLERALDLVTTLQPVIVAVIDTGVDAEHPDLAGVVLPGTTILSSQSTGCAADAVGTD
                     DNSHGTHVAGIIAADANNGIGIAGIAPNARILPIKALDCTGSGSLADVAQAITYAVDH
                     GARVINISLGASTDSVTLESAVQYAVRRNVLIVAAVGNCGLISSRCLDTENLAEYPGA
                     SSGVLGVAATSPDDSIASFSTRGAQVALSAPGVRILSTTPHYPTFQSRRGSTMNYAAF
                     SGTSQATPFVAGAAAFLLGVDASLTASGVADRLKTTADDLGASGVDPTFGAGRIDLVR
                     AVIASLPAFSAQYDTSFVPRWVLNGTSFVAKVAITNTSRSTWSSTGGAPVELSYHWLD
                     A"
BASE COUNT          358 a          856 c          836 g          368 t
ORIGIN      
        1 tcacctgcgt ggtccgcggc tgcggcaaga tcctcgagga cctggtcacg tacgaccgca
       61 gcctcgtcat ggagacctac gcgatggcgc cccggtagcg cgtggccagc ctcggtcgca
      121 cccggcgctc gcagaacgga ttccttcgat cgttcgcggt cctgttcctc atctcgaccc
      181 tgctgctgct cgcgcgcaac tgggatccgc tgcggtcgct cgcgaccgcg gggaccgagc
      241 tgctcgtgcc ggtgcagcgc gtgctcgcgg acgcgggcat caccgtgaac cagttcacgc
      301 aggccatcag cgagatccag cggctgcgcg cggacaacgc gaagctccat gacgacgtcg
      361 accgcctcac gctcgagaac gtgcgcctgc gcgaggcggc cttcgccgcg cagcaggccg
      421 cgaagctgca gcaggccgcg gcgacgctgt ccttcgagac ggtgcgcgcg acggtcatcg
      481 cgcgcgatcc ctcgaacatc ctcgccacgc tcgtgctcgg cgtgggcgcg aacgatggcg
      541 tgaaggccgg ccacgtcgtc gtgtcggacc aggggctcgt gggacgcatc tccgaggtgg
      601 gaccgagcta cagcaaggtc ctgctgctca cggacccggc gtcctcgctt tccgcgctcg
      661 tcgagggctc ccgcgccacc gggatcgtgc gcggccagta cggcgacgcg ctgatcatgg
      721 agtggatcct ccagaccgag gcggtgaagc ccggcgacgt cgtcgtcacc gcggggctcg
      781 ggctgggcga cgagctgcgc tccctgtacc cgaaggggct cgtggtgggg aagatcgtcc
      841 agctgcagaa cgccgaggcc gccgcgtatc aacgggcgat catcgtcccc gcggtggacc
      901 tccgccgcct ggagaacgtg ctcgtcgtac gaacgacccc ataacttccc gccggcagac
      961 cgaataatcg tggtgtggaa ggaagggcgg ctcgccgtct tggcgttgcc ctgctcgcgg
     1021 cagcgctttg cgcgagccag ccgtcggtcg cccgcgcggc aagcgtgccc acggagacgc
     1081 tgcacctgct cgttcagttc gccgccggta ccagtgacgc cgaccggacg gcgatcgcgc
     1141 gggatctcgg cgggacgatc gagggggaga tccgcccgct gggcgtgacg cgcctcgcga
     1201 tcaccgtcga gcgcggcacg gatcccgagg cggtcctgcg cgcgatcgcc gccaaccgga
     1261 aggtcacgac cgcggaacaa gacgcacgcg tacacctcga cgggactccg aacgatcagt
     1321 actgggacac ggacccgttc gtcggtctgg gtcaatgggg cgcgaagaag gtgatgctcg
     1381 agcgcgcgct cgacctcgtc acgacgctcc agccggtcat cgtcgcggtc atcgacaccg
     1441 gcgtcgacgc ggagcatccc gatctcgccg gcgtggtgct tcccggcacc acgatcctga
     1501 gctcgcaatc gacgggttgc gccgcggatg ccgtcggcac ggatgacaac tcgcacggca
     1561 cccacgtcgc ggggatcatc gcggccgacg cgaacaacgg catcggcatc gcgggcatcg
     1621 cgccgaacgc gcggatcctc ccgatcaagg cgctcgattg caccggcagc ggatcgctgg
     1681 cggacgtcgc gcaggcgatc acgtacgccg tcgaccatgg cgcgcgcgtc atcaacatct
     1741 cgctcggcgc gtcgaccgac agcgtgaccc tcgagtcggc ggtgcagtac gccgtgaggc
     1801 gcaacgtgct catcgtggcg gccgtcggga actgcgggct gatctcgtcg cgctgcctcg
     1861 ataccgagaa cctcgccgag taccccggcg cgtcgtccgg cgtgctcggt gtcgcggcga
     1921 cctcgcccga cgattcgatc gcgtccttct cgacgcgcgg tgcgcaggtc gcgctcagcg
     1981 cgccgggcgt tcgcatcctg agcacgacgc cccactatcc gaccttccag tcgcggcgcg
     2041 gcagcacgat gaactacgcg gcgttctcgg gaacgtcgca ggcgacgccc ttcgtcgccg
     2101 gcgccgcggc gttcctgctc ggcgtcgatg cgtccctcac cgcgtccgga gtcgcggatc
     2161 ggttgaagac gacggccgat gatctcggcg cgagcggcgt cgatccgacg ttcggcgccg
     2221 ggcgcatcga cctcgtacgc gccgtcatcg cgtcgctgcc ggcattcagc gcgcagtacg
     2281 acacgagctt cgtgccgcgg tgggtgctga acggcacctc gttcgtggcg aaggtcgcga
     2341 tcacgaacac ctcgcgttcg acgtggtcgt cgacgggtgg ggcgccggtc gagctctcgt
     2401 atcactggct cgacgcga
//
LOCUS       DASTZR010000051         3000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_812136, whole genome shotgun sequence.
ACCESSION   DASTZR010000051 DASTZR010000000
VERSION     DASTZR010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812136"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VFV20_01125"
     CDS             <1..385
                     /locus_tag="VFV20_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286981.1"
                     /translation="MNLGAARVEAGDVERGRDDLRRALAQLDGTKFLPGAHRDLAVAE
                     LAAGDVVAAAAHAEQALSLARTAGARQTEAQAERVLAQIAIARGDEDQARELLARSRK
                     TFSELGEEAELARTEAVPLKREGSS"
     gene            complement(382..2934)
                     /locus_tag="VFV20_01130"
     CDS             complement(382..2934)
                     /locus_tag="VFV20_01130"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HEU5286982.1"
                     /translation="MRRDTRLARLYAEWELQPAKRCVAFANVIVESIREIIADFAAHN
                     PLRYVVLVGGDDVIPFARIEDRAEISREWQWAGPYDSETPLGAALTYGYFLSDDPYGR
                     SGPLDQRGKNVRTPTIAVGRLVESVDDIRAYLDWYARQGCLRGCVGAFLPWRGKRTAT
                     IKLESALVTGYTFVHDLGDEVKGALANAGTSVDDSLVNDVWTADQLANKIKDRNQLIA
                     AQGHYSANTLVPAAAGTPKLVPTDPSIAKGDLAGRIWLTIGCHSGHNIVDRETRQAYR
                     HPTSWPEALLRKGVTLVGGTGYQYAESVLLENSEKLYAYLTQELVGANEVAIGAALIA
                     AKQRYLTQCLQVRGIDEKVLAVSILYGLPMLKVRPKTAVKPAQITPVVGTPATTSKSG
                     IRTRQIELVSGLSDSDVKQLDGRDYFEASGQRVARPLRPVLPAVYRDITAGDHRPMGL
                     LWTEGTYVESDHHPLIALPVLEERSAKRPRYRNRAFLPARPFRVARAGASQTLVFAPM
                     QYRYERPDPPEGRRRLWQSAKATVYYSSRTDRSALLDAPALRDPRLAVVGGRLDATIA
                     VRHYDSEAEDVRVFASYLDGSDLRTVALTAAAPSAADGIGFVRTFNGPVPAVPGVAPE
                     QQRVFFQAVGANGRVSALMNGGRLFGVSRAATQATTLTLQAQATAGYRERIQVTAKLA
                     LAATGAPADGEVLFRLGGSRLWARTAGGVATVTLNVATRPRTGTSYRVTASFPGEGDL
                     SGSSASRDVIVGKARTELIAESGITLIRGERTAVARLQLASDPARVLGLRPIVLRPGK
                     DERVAYTDQDGVLRVEPSEVGESDEVELRFEGDDRYESASATVGIGRVRMRS"
BASE COUNT          450 a         1035 c         1051 g          464 t
ORIGIN      
        1 gatgaatctc ggggcggcgc gcgtcgaggc gggtgacgtc gaacgcggcc gcgacgatct
       61 gcgacgagcc ctcgcgcagc tcgatgggac gaagttcctg ccgggtgcgc atcgtgacct
      121 cgccgtcgct gagctcgccg ccggcgacgt cgttgccgca gccgcccacg cggagcaggc
      181 gctgagcctc gcgcgcaccg cgggcgcgcg acagacggag gcacaggccg agcgcgtcct
      241 cgcgcagatc gcgatcgccc gcggtgacga ggaccaggcg cgtgagctgc tcgcacgcag
      301 ccggaagacg ttcagcgagc tcggcgagga agcggagctg gcgcggacag aggctgttcc
      361 attgaagcgc gaaggatcgt cctagctccg catacgcacg cgcccgatcc cgaccgtggc
      421 cgaggcgctc tcatacctgt cgtccccttc gaaccgcagc tcgacctcat cgctctcccc
      481 gacctccgac ggctcgacac gcaggacccc atcctggtcc gtgtatgcga ctcgctcgtc
      541 tttgcccggc cgcagcacga tggggcgaag gccgaggaca cgcgctggat cggacgcgag
      601 ctgcaggcgg gccacggccg tgcgctcgcc ccggatgagc gtgatgccgc tctcggcgat
      661 gagctccgtg cgagctttcc cgacgatcac gtcccgagac gcgctggagc ccgatagatc
      721 cccttcgccc gggaacgatg cggtgacccg gtaggaggta ccggtgcgcg gccgtgtcgc
      781 gacgttgagc gtcaccgtcg cgacgccgcc cgcggtcctc gcccagagtc gcgatccgcc
      841 gaggcggaag aggacctcgc catcggcggg cgcccccgtc gcggcgagcg cgagcttcgc
      901 cgtgacctgg atccgctcgc ggtagccggc cgtggcctgc gcctggagcg tgagcgtggt
      961 cgcctgcgtg gccgcgcgcg acacgccgaa cagccgtcca ccgttcatga gcgcggatac
     1021 gcgcccgttg gcgccgaccg cctggaagaa cacgcgctgc tgctcgggcg ccacgccggg
     1081 caccgccggc accggaccgt tgaacgtgcg gacgaagccg atcccgtcgg ccgcggaggg
     1141 cgcggcggcc gtgagcgcca ccgtccggag atccgacccg tcgaggtagg aggcgaagac
     1201 ccgcacgtcc tcggcctccg agtcgtagtg gcggacggcg atcgtcgcat cgagccgtcc
     1261 gccgaccacc gcgaggcgcg gatcgcgtaa cgccggcgcg tccagcagcg cgctgcgatc
     1321 cgtgcggctc gagtagtaga cggtggcctt cgcgctctgc cacagacgcc gcctgccctc
     1381 gggtgggtcc ggccgctcgt agcgatactg catgggcgcg aacacgagcg tctgggacgc
     1441 cccggcgcgc gcgacgcgga agggtcgcgc cgggaggaac gctcggttgc gataccgcgg
     1501 ccgcttcgcc gacctctcct cgagcaccgg cagggcgatg agcgggtggt gatcgctctc
     1561 gacgtaggtg ccttcggtcc acagcagacc catgggcctg tggtcgcctg ccgtgatgtc
     1621 gcgatacacg gccggaagca cggggcggag cggccgagcg acacgttggc cagaagcctc
     1681 gaaatagtca cggccgtcga gctgcttcac gtccgagtcc gagagtcccg acacgagctc
     1741 gatctgtcgc gtgcggatgc cgctcttact ggtcgtggcg ggcgttccga cgaccggtgt
     1801 gatctgcgcg ggcttcaccg cggtcttcgg gcgcactttc agcatcggca gcccgtacag
     1861 gatcgatacg gccagcacct tctcgtcgat gccgcggacc tgtaggcatt gcgtgagata
     1921 ccgctgcttg gcggcgatca gcgccgcacc gatcgcgacc tcgttcgcgc cgacgagctc
     1981 ttgcgtgagg tatgcgtaca gcttctcgct gttctcgagg aggaccgact cggcatattg
     2041 gtagcccgtg ccgccgacga gcgtcacgcc cttgcgcagg agtgcctccg gccacgacgt
     2101 cggatgccgg taggcctgac gtgtctcgcg atcgacgatg ttgtgtccgc tgtggcagcc
     2161 gatcgtcagc cagatccgtc cggccaggtc tcccttcgcg atgctggggt ccgtcggaac
     2221 gagcttcggc gtaccggcag cggccgggac gagcgtgttc gccgagtagt gaccctgcgc
     2281 cgcgatcagc tgattgcggt ctttgatctt gttcgcgagc tggtcagccg tccagacgtc
     2341 gttgacgagc gaatcgtcga cgctcgtgcc ggcattcgcc agcgcaccct tgacctcgtc
     2401 ccccaggtca tgcacgaagg tgtagccggt gacgagtgcg ctctcgagct tgatggtcgc
     2461 agtgcgcttt ccgcgccagg gaaggaaggc accgacgcag ccgcggaggc atccctggcg
     2521 cgcgtaccaa tcgaggtagg cgcgaatgtc gtccacggac tcgacgaggc gcccgaccgc
     2581 gatcgtaggt gtacgcacgt tcttcccgcg ctgatcgagc ggaccgctgc gtccgtaggg
     2641 gtcgtctgag aggaagtacc cgtacgtgag cgccgcgccg agcggggtct cggagtcgta
     2701 cggacccgcc cactgccact cgcgcgagat ctcggcacga tcctctatgc gcgcgaacgg
     2761 gatcacgtcg tcaccgccga cgagcacgac gtaacgcaac ggattgtgcg cggcgaagtc
     2821 ggcgatgatc tcgcggatcg attcgacgat gacgttcgcg aacgcgacgc accgctttgc
     2881 gggctgcagc tcccactccg cgtagagccg cgcgagacgt gtgtcgcgcc gcaggtctta
     2941 caccgagccg ctcacgcggg cggcgaggtc cccgagggtc cgcttcacga acgtgtcccg
//
LOCUS       DASTZR010000052         2013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_682377, whole genome shotgun sequence.
ACCESSION   DASTZR010000052 DASTZR010000000
VERSION     DASTZR010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682377"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..173)
                     /locus_tag="VFV20_01135"
     tRNA            complement(100..173)
                     /locus_tag="VFV20_01135"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(137..139),aa:Val,seq:gac)
     gene            259..546
                     /locus_tag="VFV20_01140"
     CDS             259..546
                     /locus_tag="VFV20_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286983.1"
                     /translation="MRRMRPRALTPDEAADLAAGLDEDEIDGRIETICLAPTIDATAT
                     LSEAAFELHAFALWLADLERDGWQMVEDAGADAHLRVINADSRKRVYPADA"
     gene            complement(543..1646)
                     /locus_tag="VFV20_01145"
     CDS             complement(543..1646)
                     /locus_tag="VFV20_01145"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEU5286984.1"
                     /translation="MALVDRLVARIRARGPMTFADYMDAALYDPEEGYYSTRVALGFE
                     GDYLTAPDLGPHFGRSLARAFADCWTLLGKPAVWDVVEAGAGRGTLLRDVLLTLERER
                     PDVARGVRPAIVEVSPRLRAEQSAALEGRDLRWASDPRALAPLQGLVYANEILDAFPV
                     HVLVRADDGIREVHVDESGGRLVEVLRPPSSPDLRYRVPESLPRGGRWEVSPEAEGWV
                     ASLAAALTRGYVIFIDYGGDESDLLMRMGSGTVRGFARHRFVGDPYAEPGAHDLTASV
                     NFTAIRRAAEGAGFAFAGRTSQREALLALGIRDVASRPTSPVEQLRDYGRRSAIDTLL
                     EPSGFGGFQVMCFAKDAPTDGLRMFSGREARAR"
     gene            complement(1707..>2013)
                     /locus_tag="VFV20_01150"
     CDS             complement(1707..>2013)
                     /locus_tag="VFV20_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU5286985.1"
                     /translation="CPTGALMMRERDLQTYELDVVRCIYCGDCVEVCPHGALGETPNF
                     ELSSYQRFGHTVLAKEELAVARTWKPSEHIPVGEEETHRSSVRPPEVRPPAPPRWSA"
BASE COUNT          312 a          719 c          714 g          268 t
ORIGIN      
        1 cgacgccaga tcgcggtgac gaggcgcacg catcgtgtct acggtcttgg gccgccgacc
       61 tcaagcaggc aggccgtgca gggatccttg cggacgctgt gggctctgcg tggctcgaac
      121 acgcgacctc agcgatgtca acgctgcgct ctaaccagct gagctaagag ccctcgctgt
      181 ccccaaggat acccgttcgg ccgcacgcaa gtcgcggacg atcactgtac gcggcataca
      241 tgcggcttgg aggtcccggt gagacggatg cgcccgcgcg cgctcacacc cgacgaagcg
      301 gccgacctgg cggcgggcct cgatgaggat gagatcgacg gccgcatcga gaccatctgc
      361 ctcgcgccga cgatcgacgc gaccgcgacg ctgtcggagg ctgccttcga gctgcacgcg
      421 ttcgcgctgt ggctggcgga cctcgagcgc gacggctggc agatggtcga ggatgccggc
      481 gccgacgcgc atctgcgcgt gatcaacgcg gactcgcgca agcgcgtgta tcccgccgac
      541 gcctagcgcg ctcgcgcctc gcggccgctg aacatgcgca ggccgtcggt cggcgcgtcc
      601 ttggcgaagc acatcacctg gaagccgccg aagccgctgg gctcgaggag cgtgtcgatg
      661 gccgaccggc gcccgtagtc gcggagctgc tccaccggcg acgtcggacg cgacgcgacg
      721 tcacggatgc cgagcgcgag cagcgcctcc cgctgggacg tgcggcccgc gaacgcgaac
      781 cccgcgcctt cggccgcgcg gcggatcgcc gtgaagttca cgctcgccgt gaggtcgtgc
      841 gcgccgggct cggcatacgg gtcgccgacg aaacggtgcc gcgcgaaacc gcgcaccgtg
      901 ccggagccca tgcgcatcag caggtcggac tcgtcgccgc cgtagtcgat gaagatgacg
      961 tagccgcggg tgagcgcggc cgcgaggctc gcgacccacc cctctgcctc cgggctcacc
     1021 tcccatctcc cgccgcgcgg caggctctcc gggacgcgat agcgaagatc cgggctcgac
     1081 ggcgggcgga gcacctcgac caggcggccg ccgctctcat ccacatgcac ctcgcggata
     1141 ccgtcatcgg cgcgcacgag cacgtgcacg gggaacgcgt cgaggatctc gttcgcgtag
     1201 acgagaccct gcagcggcgc gagggcccgc gggtcggagg cccagcgcag atcgcgaccc
     1261 tccaacgcgg cggactgctc cgcgcgcagc cgcggcgaga cttcgacgat cgccgggcgg
     1321 acgccgcgcg cgacatcggg gcgctcgcgt tcgagggtga ggaggacgtc gcgcaggagg
     1381 gtcccccgcc ccgcgccggc ctcgacgacg tcccagaccg cgggcttgcc cagcaacgtc
     1441 cagcagtccg cgaacgcccg cgcgagcgag cgaccgaagt gcggtcccag atccggtgcc
     1501 gtgaggtagt cgccctcgaa gccgagcgcc acgcgggtgg agtagtagcc ctcctcgggg
     1561 tcatagagcg cggcgtccat gtagtccgcg aacgtcatcg gtccccgtgc gcggatccgc
     1621 gcgacgagac gatcgacgag tgccacgagg tcagcgtatc gccgtcagcc gatcaacaac
     1681 tgccgggact cgcctgtcga gcgcgctcag gcgctccaac gcggtggtgc gggcggacgg
     1741 acctcgggcg ggcgcacgct gctgcgatgc gtctcctcct cgccgacggg aatgtgctcg
     1801 gacggcttcc acgtccttgc gacggcgagc tcctccttgg cgaggaccgt gtggccgaac
     1861 cgctggtacg aggagagctc gaagttgggc gtctcgccga gggcgccgtg cgggcacacc
     1921 tcgacgcagt cgccgcagta gatgcaccgc accacatcga gctcgtacgt ttgcagatcg
     1981 cgctcgcgca tcatgagcgc gcccgtcggg cag
//
LOCUS       DASTZR010000053         4599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_134098, whole genome shotgun sequence.
ACCESSION   DASTZR010000053 DASTZR010000000
VERSION     DASTZR010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134098"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1783
                     /locus_tag="VFV20_01155"
     CDS             <1..1783
                     /locus_tag="VFV20_01155"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEU5286986.1"
                     /translation="KHRAVNLTEEGIARAERLLGVQNMYEGDVTSVHYLDNAVKAKAL
                     YLKDREYVVKDGEVVIVDEFTGRLMPGRRWSDGLHQAVEAKERVKIQRETRTFATITI
                     QNYFRMYKKLAGMTGTAATEAEELFKVYKLEVTVIPTNRPMVRKDEPDLVYKTEQGKW
                     NAVVADVQERNRAGQPVLIGTTSVDKSQRLSDELRRHGIKHEVLNAKNHEREALIIAG
                     AGQPHAVTLSTNMAGRGVDILLGEGVKELGGLHVIGTERHEARRIDNQLRGRAGRQGD
                     PGSTRFFNSLEDDLIRVFASERVAGLMARLGFDDTQPIESGMVTGAITQAQIKVEARN
                     FDIRKRALEFDDVLNNQRQVIYGERRKILEKGDVRETVLGYLQDEAAALVDAEAATPD
                     PEDWDLEKLALAVGALLGRTDITAGSFKDIQSPDELGDKVAEIVDTTYEARETEFGPE
                     ISRAIERWVLLRTIDTHWIEHLTAMEELREGIYLRGYGQQDPLVAYKREAHDFFEQMR
                     SRIAAGVAQTVMRVSVRTAEQAEQAEQQKPQTTSTSGGTTTTGRADLRTNRDDNSTTT
                     RPANEKIGRNDPCWCGSGKKFKKCHGR"
     gene            1794..2639
                     /locus_tag="VFV20_01160"
     CDS             1794..2639
                     /locus_tag="VFV20_01160"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEU5286987.1"
                     /translation="MIIDLDPNIHLGPLTLAWHGIFTAVGIAFGVWLSTKLVMARVSE
                     ADATAVATWGVVGGIIGARLVHVFDCWTGCTGIPGGYSENPLLIPQIWTGGIAIWGAF
                     VGGVLGGLIVALRRGTVPIGFTADGAAPGIALGFAIGRIGDIINGEHHAIACQAPLGL
                     CVEFTNPATLGQGSTFAVDDWRYAAGPVHLVVAYDMAWNVAAVIGALLLRGRGLPDGL
                     IFWLYAAWYAIGRFLLGYLRVGDPSYAFALREDQVIGVIVFAAAVPMIVRLWPRRPRT
                     VATTT"
     gene            2653..3426
                     /locus_tag="VFV20_01165"
     CDS             2653..3426
                     /locus_tag="VFV20_01165"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEU5286988.1"
                     /translation="MIDIPFDPDLVIGSFRVSWHSLFAFVGMIAGSIVSFRAARYLVK
                     DDRVYPFAIAVVVGGIAGARAAHIVDNWSFYDGDVLEMFDLSRGGIGTMGAPIGSTVA
                     GLVAGRWLRLPLGFMFDVSVIGIALGEAIGRVGDIINGEHHAVACAGLPWCVRYTSPS
                     TLGQSSAVHPIGAYDALLMLGIFVVLFLFWRRVRGRPPESRVYWAYLLLLGAGRFLQS
                     YVRIEPAVVWGWTEAQILGLLYALAGAAMLVVLTRRSRA"
     gene            3641..3838
                     /locus_tag="VFV20_01170"
     CDS             3641..3838
                     /locus_tag="VFV20_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286989.1"
                     /translation="MKEWIVCEDNCLKCSYGATLDCDGDCPRCPRNTYCPCVTFVSFA
                     AGKLAPVAQPVMTTFERPRKR"
     gene            3933..>4599
                     /locus_tag="VFV20_01175"
     CDS             3933..>4599
                     /locus_tag="VFV20_01175"
                     /inference="COORDINATES: protein motif:HMM:NF015427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HEU5286990.1"
                     /translation="MEKLEADSGRPDLWNDRQRAQQLLKRLGMLRTRVERWDGFRKRS
                     EDLVELASLEDAALSADLESEFATLSDDVNKAMLEAQLSGPYDERDAVLAISAGVGGT
                     DAADWAQMLVRMYLRWAERKGYRAEIVETTPGDEAGIRSATLTIEGPYAYGYLRTERG
                     THRLVRISPFDAQRRRQTSFALVDPTPQIESEGEVEIRPEELRIDTYRATGAGGQNVN
                     KTET"
BASE COUNT          752 a         1577 c         1586 g          684 t
ORIGIN      
        1 gaagcatcgg gcggtgaacc tcaccgagga ggggatcgcg cgggcggagc gtctgctggg
       61 cgtgcagaac atgtacgagg gcgacgtcac ctcggtccac tacctcgaca acgccgtgaa
      121 ggcgaaggcg ctgtacctga aggaccgcga gtacgtcgtg aaggacggcg aggtcgtcat
      181 cgtcgacgag ttcaccggcc ggctcatgcc cggccgccgc tggagcgacg gcctgcacca
      241 ggcggtcgag gcgaaggagc gcgtgaagat ccagcgcgag acgcgcacct tcgcgacgat
      301 cacgatccag aactacttcc gcatgtacaa gaagctcgcc ggcatgaccg gtacggccgc
      361 gacggaggcc gaggaactct tcaaggtcta caagctcgag gtcacggtca tcccgacgaa
      421 ccgtccgatg gtccggaagg acgagcccga cctcgtgtac aagaccgagc aggggaagtg
      481 gaacgcggtc gtcgccgacg tgcaggagcg caatcgcgcg ggccagccgg tgctcatcgg
      541 cacgacctcg gtggacaagt cacagcggct ctcggacgag ctgcgccgtc acggcatcaa
      601 gcacgaggtc ctgaacgcga agaaccacga acgggaggcg ctcatcatcg ccggcgccgg
      661 ccagccgcac gcggtcacgc tctcgaccaa catggccggc cgcggcgtcg acatcctcct
      721 cggcgagggg gtcaaggagc tcggcggcct gcacgtcatc ggcaccgagc gccacgaggc
      781 ccgacgcatc gacaaccagt tgcgcggccg tgccggccgc cagggcgacc ccggatcgac
      841 gcgcttcttc aactcgctcg aggacgacct catccgcgtc ttcgcgtcgg agcgggtcgc
      901 gggactgatg gcgcggctcg gcttcgacga cacgcagccg atcgagtcgg gcatggtcac
      961 cggcgcgatc acgcaggcgc agatcaaggt cgaggcgcgc aacttcgaca tccgcaagcg
     1021 cgcgctcgag ttcgacgacg tcctgaacaa ccagcgccag gtcatctacg gcgagcggcg
     1081 caagatcctc gagaagggcg acgtccggga gaccgtcctg ggctacctgc aggacgaggc
     1141 cgccgcgctc gtggacgccg aggcggccac gccggacccg gaagactggg acctggagaa
     1201 gctcgcgctc gccgtcggtg ccctccttgg tcgcaccgac atcacggcgg gatccttcaa
     1261 ggacatccag tcgccggacg agctcggcga caaggtggcc gagatcgtcg acacgaccta
     1321 cgaggcgcgg gagacggagt tcggtccgga gatctcgcgc gcgatcgagc gctgggtgct
     1381 gctgcgcacg atcgacacgc actggatcga gcacctcacg gccatggagg agctgcggga
     1441 gggcatctac ctgcgtggct atggccagca ggacccgctc gtggcgtaca agcgcgaggc
     1501 gcacgacttc ttcgagcaga tgcgctcgcg catcgcggcc ggcgtcgcgc agacggtgat
     1561 gcgcgtatcc gtccgcaccg cagagcaggc cgagcaggcg gagcagcaga agccgcagac
     1621 gacgtcgacc agcggcggca ccacgacgac cggtcgcgcc gatctgcgca ccaaccgcga
     1681 cgacaacagc acgacgacgc ggcccgcgaa cgagaagatc ggccgcaacg acccgtgctg
     1741 gtgcggatcg ggcaagaagt tcaaaaagtg ccacggccgc taaggctcag cgagtgatca
     1801 tcgatctcga tcccaacatc cacctcgggc cgctcacgct cgcgtggcat ggcatcttca
     1861 cggcggtcgg gatcgccttc ggcgtctggc tctccacgaa gctggtcatg gcccgggtca
     1921 gtgaggcgga tgcgaccgcg gtggcgacct ggggcgtcgt cggcggcatc atcggcgccc
     1981 gcctcgtgca cgtcttcgac tgctggaccg gctgcaccgg catcccgggc ggctactcag
     2041 agaaccccct gctcatcccg cagatctgga ccggcggcat cgcgatctgg ggcgccttcg
     2101 tcggcggggt cctgggcggg ctgatcgtgg cgctgcgacg cggcaccgtg ccgatcggtt
     2161 tcaccgcgga cggcgccgcg ccgggcatcg cgctcgggtt cgccatcggc cgcatcggcg
     2221 acatcatcaa cggcgagcac cacgcgatcg cgtgccaggc gccgctcggc ctctgcgtcg
     2281 agttcacgaa ccccgcgacg ctcggtcagg gctccacctt cgcggtggac gactggcgct
     2341 acgccgccgg accggtccac ctcgtcgtcg cctacgacat ggcgtggaac gtggccgcgg
     2401 tcattggcgc gctgctcctg cgcgggcgcg ggctccccga cgggctcatc ttctggctct
     2461 acgcggcgtg gtacgcgatc ggccggttcc tgctcggcta ccttcgcgtc ggggatccga
     2521 gctacgcgtt cgcgctccgc gaggaccagg tcatcggcgt gatcgtgttc gccgcggccg
     2581 tcccgatgat cgttcggctg tggccgcgtc gcccgcgcac ggtggccacg accacctgat
     2641 cgtcgcgcga ccatgatcga catcccattc gatcccgacc tcgtcatcgg ctcgttccgg
     2701 gtgtcgtggc actcgctctt cgcgttcgtg gggatgatcg ccggcagcat cgtctccttc
     2761 cgcgccgcgc ggtacctcgt gaaggacgat cgtgtgtatc cgttcgccat agcggtcgtc
     2821 gtcggcggca tcgccggcgc gcgcgccgcg cacatcgtgg acaactggtc gttctacgac
     2881 ggtgacgtcc tcgagatgtt cgacctgtcg cgcggtggca tcggcacgat gggcgcgccg
     2941 atcggctcga ccgtcgccgg gctggtggcc ggccgctggc tgcgtctgcc gctcggcttc
     3001 atgttcgacg tcagcgtcat cggcatcgcg ctcggcgagg cgatcggacg ggtcggcgac
     3061 atcatcaacg gcgagcatca cgccgtcgcc tgcgccggcc tcccgtggtg cgtgcgctac
     3121 acgagcccgt ccacgctcgg gcagtcaagc gcggtccacc cgatcggtgc gtacgacgcg
     3181 ctgctcatgc tcggcatctt cgtcgtgctg ttcctgttct ggcggcgtgt ccgcggccgt
     3241 ccgcccgaga gccgcgtgta ctgggcgtac ctgctgctgc tcggcgcggg ccggttcctg
     3301 cagagctacg tccggatcga gccggcggtc gtctggggat ggaccgaggc gcagatcctc
     3361 gggctgctct acgccctggc gggcgccgcc atgctcgtcg tccttacccg ccgttcgcgc
     3421 gcctgagccg caggcctccc cggctcgccg aagtgttcgc agagcgggcg ggcacgcgcg
     3481 taccaagcaa aaacctcttc acaaagcggt ttcagcccgg taggctcgta cggaaagcgg
     3541 cacacgtccc gtggggagca gcgagccgat cgggggacgc agccggaaca cctgcgatgt
     3601 tgctggcccc gtacgggcct agagaagagg acagccccgc atgaaggaat ggatcgtctg
     3661 cgaggacaac tgcctcaagt gctcgtatgg cgccacgctc gactgtgatg gcgactgccc
     3721 gcgctgcccg cgcaacacgt actgcccctg tgtcacgttc gtgagcttcg ccgccggcaa
     3781 gctcgcaccg gtcgcgcagc cggtgatgac gaccttcgag cgcccgcgca agcgatagtc
     3841 ggtgtccatc gacccggagg cgctcgcggc gctgcgcgaa cgcatcgacc gcatggcggt
     3901 gttcctttga cctccccggt attcgcaccg agctggagaa gctagaggcg gattcgggac
     3961 gccccgacct ctggaacgac cgccagcgcg cacaacagct cctgaagcga ctcggcatgc
     4021 tccgcacgcg tgtcgagcgg tgggacggat tccggaaacg ctctgaggat ctcgtcgagc
     4081 tcgcgtcact cgaggacgcg gcgctctccg ccgaccttga gagcgaattc gccacgctct
     4141 ccgacgacgt caacaaggcg atgctcgagg cgcagctgtc cggaccatat gacgagcgcg
     4201 acgcggtgct cgccatcagc gcgggcgtgg gcgggacgga cgccgcggac tgggcgcaga
     4261 tgctggtacg catgtatctg cgctgggccg agcgcaaggg gtatcgggcg gagatcgtcg
     4321 agaccacccc gggtgacgag gccggcatcc gcagcgcgac gctcaccatc gagggcccgt
     4381 acgcgtacgg ctacctacgc acggagcgcg gcacgcatcg tctcgtgcgc atctcgccat
     4441 tcgacgcgca gcggcggcgg cagacctcgt tcgcgctcgt cgatcccacc ccgcagatcg
     4501 agagcgaggg cgaggtcgag atccgtcccg aggagctgcg catcgacacg taccgcgcga
     4561 ccggggcggg cggacagaac gtgaacaaga cggagacgg
//
LOCUS       DASTZR010000054         3460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_447456, whole genome shotgun sequence.
ACCESSION   DASTZR010000054 DASTZR010000000
VERSION     DASTZR010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447456"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..1549
                     /locus_tag="VFV20_01180"
     CDS             236..1549
                     /locus_tag="VFV20_01180"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEU5286991.1"
                     /translation="MGAGAAIVLTVFLVLVNGFFVAAEYSFVRVRGTQLDELAKTGSG
                     RARLAARMSADIEPYISTVQLGVTLASLGVGLLGEPAVSRLVDPLFGWLRDLSETAFH
                     VLSFLIAFSLVTYVTVVFGELAPKYLAIQRALQVALWTAYPLHFTYRAAYPFIWLMNK
                     SAAAVLRAAGGKPTGDLDVHSDEELKMLVAASARQGVLQESERLIVGNALDFADTVVR
                     QVMIPRTEIAAAPEDLDLGGLIALARQSRFSRFPVYRDDLDHVVGVVHVKDLVGVDRT
                     SHATARDLMRRVPFIPETLRLDQALAEFRRQRAGLAVVVDEFGGTAGVVTLADIMEEL
                     VGEVSDEFERGEAQRFREEAPGTFLIDGLVALDDLRDRLQLSLEGEPYDTVGGLVFGR
                     LGRLAEAGDVVEVEGYRFQVTTIDGRRVSQVRVQRLRAPRRAAPS"
     gene            1622..1762
                     /locus_tag="VFV20_01185"
     CDS             1622..1762
                     /locus_tag="VFV20_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286992.1"
                     /translation="MIEMLLSDPVIFLGTTVGLCVTALSIVRDARSQRARSRAEEVRV
                     EA"
     gene            complement(1759..1941)
                     /locus_tag="VFV20_01190"
     CDS             complement(1759..1941)
                     /locus_tag="VFV20_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286993.1"
                     /translation="MDDRRRLAPRASALSFVVTCALATAAATGLFVLLDPRAQAFWAA
                     RISEFVAAVQGLFGAR"
     gene            complement(1950..2891)
                     /locus_tag="VFV20_01195"
     CDS             complement(1950..2891)
                     /locus_tag="VFV20_01195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU5286994.1"
                     /translation="MLTSPTTSKGKSFRDAGSRIFTQETDAQPGIISLLAARTPTAVR
                     EIPLEKVKPNPGQPRMTFHQDTLNELASSVKEHGVLQPILVRPSGEGYEIIAGERRWR
                     ASKLAGKETIPAIVERFDDATALEIALIENLQREDLSPLDESTIYKKMTDELGYSIRQ
                     LAGKLGKDKGYVENRLRLANAPDDVREMVSQRYDTLSAAYELMKLDNPRRRKALAKQM
                     LAGQLTLIKLHDRVERILNPQAKAEKPEPAIPPLRDDALITATRKLNESLADLSRAIG
                     DDDSATNIAEGDRQNLAKFLTISRARLDNLVARLKSR"
     gene            complement(2881..>3460)
                     /locus_tag="VFV20_01200"
     CDS             complement(2881..>3460)
                     /locus_tag="VFV20_01200"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEU5286995.1"
                     /translation="LVDPDVSLESIIRPSKTRNVDVVPATLELSSAEVELFNAIGREM
                     ALRDKLAPAIAQRYDYILIDCPPTLGLLTLNALVAADGVIIPVQTQYYALKGVAALLR
                     IIKTVQARLNPNLKVYGLLPTFYDSRTILARDMLDSLKDLGDHQVFATVIRQSVKIGE
                     APTAGLPITEYDKKSDAAKAFVDLAKEVVRAH"
BASE COUNT          481 a         1143 c         1224 g          612 t
ORIGIN      
        1 gggtggcgcc tcacgatctc ggcgatcgca tcgcgcaccg tggcgcccgg cggcagctcg
       61 atctccatgc gtcccacacc cgcggcttcg cggtacgacg cgaagagacg gaggaccacg
      121 cgcacgtccc gagtgtattt cgtaatgggc gagcgaggag cggcttcgcc gcgacgagcg
      181 agtcctgccc gcgaaacgac aacgagcgct ggaatcagtg cgagaatgag tcggaatggg
      241 cgcgggcgcg gcgatcgtcc tcaccgtctt cctcgtcctc gtgaacgggt tcttcgtggc
      301 tgcggagtac tccttcgtcc gcgtccgggg cacgcagctc gacgagctcg cgaagaccgg
      361 cagcggccgc gcgaggctcg ccgcacgcat gtcggcggac atcgagccct acatctcgac
      421 cgtgcagctg ggtgtcacgc tggcctcgct cggcgtcgga ttgctcggcg agccggcggt
      481 ctcgcgactc gtcgacccgc tgttcggatg gctccgcgat ctgtccgaaa ccgcgttcca
      541 cgtgttgagc ttcctcatcg cgttcagcct ggtgacctat gtgacggtgg tgttcggcga
      601 gctcgctccc aagtacctcg ccatccagcg cgccctgcag gtcgcgctgt ggaccgcgta
      661 cccgctccac ttcacgtacc gggcggccta tcccttcatc tggctgatga acaagagcgc
      721 ggcggcggtg ctccgcgcgg ccggcggcaa gccgaccggc gacctcgacg tccacagcga
      781 cgaggagctc aagatgctcg tcgcggcgtc ggcgcggcag ggcgtgctgc aggagagcga
      841 gcggctcatc gtgggcaacg cgctcgactt cgccgacacc gtcgtcaggc aggtgatgat
      901 ccctcgcacc gagatcgcgg cggcgccgga ggacctcgat ctcggcgggc tcatcgcgct
      961 cgcgcgccag agccgcttct cgcgcttccc ggtctatcgc gacgacctcg atcatgtcgt
     1021 gggggtcgtg catgtgaagg acctcgtcgg cgtggatcgc acctcgcacg ccacggcgcg
     1081 cgacctcatg cggcgcgtgc cgttcatacc ggagacgctg cgtctcgatc aggcgctcgc
     1141 ggagttccgc cggcagcggg ccgggctcgc ggtggtcgtc gatgagttcg gcggtaccgc
     1201 gggagtggtg accctcgccg acatcatgga ggagctcgtc ggcgaggtga gcgacgagtt
     1261 cgagcggggc gaggcacagc gcttccggga ggaggcgccg gggaccttcc tcatcgacgg
     1321 cctcgtcgcg ctggacgatc tgcgcgaccg cctgcagctg tcgctcgaag gcgagccgta
     1381 cgacaccgtg ggcggcctgg tattcggccg actgggtcgt ctcgccgagg ccggcgacgt
     1441 cgtcgaggtg gaggggtatc gcttccaggt gacgacgatc gacggccggc gcgtgtcaca
     1501 ggtacgtgtg cagcggctcc gcgcgccgcg tcgcgccgct ccctcgtgat gtggacagtg
     1561 tgacgagcgc gtaacgacct gccacccgct tgtctggcac gggttgtgcc ttcagtcgtg
     1621 cgtgatcgaa atgcttctgt cggatccggt gatcttcctc gggacgaccg tgggtctctg
     1681 cgtcacggcg ctctcgatcg tgcgcgatgc tcgctcgcag cgtgcgcgtt cgcgcgccga
     1741 agaggtacgc gtcgaggcct agcgagctcc gaagaggccc tgcaccgccg cgacgaactc
     1801 gctgatgcgc gccgcccaga acgcctgggc tcgcgggtcg agcaggacga agaggccggt
     1861 cgccgccgcc gtggcgagcg cgcaggtcac gacgaacgag agcgcgctcg cgcgtggcgc
     1921 caggcgccgc cggtcatcca tcaccgggat tatcggctct tcagcctcgc cacgagattg
     1981 tcgagacgcg cccgcgagat cgtcaggaac ttcgcgaggt tctggcggtc gccctccgcg
     2041 atgttcgtgg cgctgtcgtc gtcaccgatc gcccgggaga ggtccgccag tgactcgttc
     2101 agcttgcggg tcgccgtgat gagcgcgtcg tcgcgcagcg gcgggatcgc gggctccggc
     2161 ttctcggcct tcgcctgggg attcaggatc cgctcgacgc ggtcgtgcag cttgatgagc
     2221 gtgagctggc cggccagcat ctgcttggcg agcgccttcc tgcggcgcgg gttgtcgagc
     2281 ttcatgagct cgtacgcggc cgaaagcgtg tcgtagcgct gcgacaccat ttcgcggacg
     2341 tcgtctgggg cgttcgcgag gcgcagccgg ttctcgacgt agcccttgtc cttgccgagc
     2401 ttgcccgcca gctggcggat cgaatagccg agctcgtcgg tcatcttctt gtagatcgtc
     2461 gactcgtcga gcggcgacag gtcctcgcgc tggaggttct cgatgagcgc gatctcgagg
     2521 gcggtcgcgt cgtcgaagcg ctcgacgatc gcgggaatgg tctccttgcc cgcgagcttc
     2581 gacgcgcgcc agcggcgctc gccggcgatg atctcgtacc cctcgccgga gggccgcacc
     2641 aggatcggct gcaggacgcc gtgctccttc accgaggacg cgagctcgtt gagggtgtcc
     2701 tggtggaagg tcatgcgcgg ctggcccggg ttcggcttca ccttctcgag cgggatctcg
     2761 cgcaccgccg tcggcgtgcg cgccgcgagg aggctgatga tccccggttg cgcgtcggtc
     2821 tcctgcgtga agatccggct ccccgcgtcg cggaacgact tgcccttcga tgtcgtgggg
     2881 ctagtgagca cggacgacct ccttggccag atcgacgaac gccttggcgg cgtccgactt
     2941 cttgtcgtat tcggtgatcg gcaggcccgc ggtgggcgcc tcgccgatct tcacggactg
     3001 acggatcacg gtcgcgaaca cctggtgatc gcccaggtcc ttcagtgagt cgagcatgtc
     3061 gcgcgcgagg atcgtgcggc tgtcatagaa ggtgggcagc agcccgtaca ccttgaggtt
     3121 cggattgagg cgcgcctgca ccgtcttgat gatccgcagc agcgcggcca cgcccttcag
     3181 cgcgtagtac tgcgtctgca ccgggatgat cactccgtcc gcggcgacga gcgcgttgag
     3241 cgtgagcagt cccagcgtcg gcgggcagtc gatgaggatg tagtcgtagc gctgcgcgat
     3301 cgccggcgcg agcttgtccc gcagggccat ctcgcgtccg atcgcgttga agagctcgac
     3361 ctcggccgac gagagctcga gcgtcgcggg caccacgtcg acgttccgcg tcttcgatgg
     3421 acggatgatc gactcgagcg acacgtccgg gtcgacgagg
//
LOCUS       DASTZR010000055         3531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_578260, whole genome shotgun sequence.
ACCESSION   DASTZR010000055 DASTZR010000000
VERSION     DASTZR010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578260"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1198)
                     /locus_tag="VFV20_01205"
     CDS             complement(143..1198)
                     /locus_tag="VFV20_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEU5286996.1"
                     /translation="MSVTTNTTAPKEEFIQRSRSTAVAGSGKNLLEVTALKMHFPLTQ
                     GIIFQRQVGAVRAVDGIDFFVEKGETLGLVGESGCGKSTTGRAILQLYKPTSGSVKFD
                     GTELTTLPGEKMRRMRRRMQMIFQDPYASLNPRMTVGNIIGEPLEVHDLAKGKAKTER
                     VQELLKIVGLNPYFTNRYPHEFSGGQRQRIGVARALAVEPDFIVADEPISALDVSIQA
                     QIINLLEELQDKFKLTYLFIAHDLSVVRHISDRVAVMYLGKIVELADRVDLYERPLHP
                     YSQALLSAVPIPDPVVETKRKRIILTGDVPSPVNPPSGCRFHTRCWKAQQICSEVDPE
                     FIEHAPRHWAACHFPGA"
     gene            complement(1206..2306)
                     /locus_tag="VFV20_01210"
     CDS             complement(1206..2306)
                     /locus_tag="VFV20_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018786437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5286997.1"
                     /translation="MALLEVNDLHTQFFTRDGVVHAVDGVTFNLEAGETLGLVGESGC
                     GKSVTALSLMRLIPQPPGKIVKGQIMFDGQDVLKMDDDDVRGIRGNNIAMIFQDPMTS
                     LNPVLTISRQISEALELHLKMDKGEARKRTIELVELVGIPSAKKRVDDYPHQFSGGMR
                     QRVMIAMALSCSPKLILADEPTTALDVTIQAQILDLLKNLAREFRTAFILITHDLGVV
                     AGMTQRIHVMYAGRIVEKADTGELFANPKMPYTWGLLRSIPRLDEQRKAKLLPIEGLP
                     PDLIAPPPGCKFEPRCQYRRDICKEKEPALIAVPNAKPDHEARCWGTQDVAGGGWLKG
                     TDWRREVGDTRLLEEIKQEAASETTTTETPGE"
     gene            complement(2355..3308)
                     /locus_tag="VFV20_01215"
     CDS             complement(2355..3308)
                     /locus_tag="VFV20_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5286998.1"
                     /translation="MATTTATASERAAQLNVLARKQRSLWRDALERLLRNKAAIAGLV
                     VILAAALIAIFAPWISPYDPLKQFSGHGQLEPLWTGSKYTEREFLLGTDQLGRDILTR
                     LIWASRVSMVVGFVPVSIVFVIGVTIGLAAGYFGGAVDQILMRVTDIVYAFPDFLFVL
                     IIVAAFRNSAFGNLLGGLVLIFAAVAIVGWIGIARLTRGQVLALKEREFIEAARSMGA
                     TSRRIMFKHLFPNALAPLIVTAAFAVPGAIIGEATLSFLGVGIKPPTPSWGAMINEGF
                     INFSANPWSVMLPALCISTVMLAFTFVGDGLRDALDPRMKS"
     gene            complement(3310..>3531)
                     /locus_tag="VFV20_01220"
     CDS             complement(3310..>3531)
                     /locus_tag="VFV20_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5286999.1"
                     /translation="VGPAAAGLITGSFIIESIFSVPGIGREYIRGIVANDYPLIMATT
                     LLYAFFIVIANLSVDLVYGMLDPRIKVGK"
BASE COUNT          567 a         1135 c         1250 g          579 t
ORIGIN      
        1 gcccgtcgcg gagcgcgtga tccggacggg ggccggccgc gtaccgccgc gcggacgtac
       61 cgctgggcgc aaagggcccg gcgagcgggc gagtcggcgg ctcgctgatg gaccctgccg
      121 ccgagggggg gccggtcgag cgctacgcgc ccgggaagtg gcaggccgcc cagtgccgcg
      181 gcgcgtgctc gatgaactcg gggtccactt ccgagcagat ctgctgagcc ttccagcacc
      241 gggtgtggaa gcggcatccc gacggcgggt tgacgggcga cggcacgtcg ccggtgagga
      301 tgatccgctt tcgcttcgtc tcgacgacgg ggtccgggat cggcacggcc gagagcagcg
      361 cctggctgta cggatggagc gggcgctcgt agagatcgac acggtccgcg agctccacga
      421 tcttcccgag gtacatcacc gcgacgcggt cgctgatgtg ccgcaccacc gacaggtcgt
      481 gcgcgatgaa gaggtacgtc agcttgaact tgtcctggag ctcctcgagg aggttgatga
      541 tctgcgcctg gatggacacg tcgagcgccg agatcggctc gtccgcgacg atgaagtccg
      601 gctcgacggc gagcgcgcgg gcgacgccga tgcgctgacg ctgcccaccg gagaactcgt
      661 gcggatagcg gttcgtgaag tacggattca agccgacgat cttcagcagc tcctgcacgc
      721 gctcggtctt cgccttgccc ttcgcgaggt cgtggacctc gagcggctcg ccgatgatat
      781 tgccgacggt catgcgcgga ttgagcgacg cgtacgggtc ctggaagatc atctgcatcc
      841 ggcggcgcat gcgccgcatc ttctcgccgg gcagggtcgt cagctcggtg ccgtcgaact
      901 tcaccgagcc ggaggtcggc ttgtagagct ggaggatcgc gcggccggtg gtggacttgc
      961 cgcagccgga ctcgccgacg aggccgagcg tctcaccctt ctcgacgaag aagtcgatgc
     1021 cgtcgacggc acgcaccgcg cccacctgcc gctggaagat gatcccctgc gtcagcggga
     1081 agtgcatctt cagcgcggtc acttcgagca ggttcttgcc gctgccggcg accgcggtgg
     1141 agcggcttcg ctggatgaac tcttccttcg gcgccgtggt gttcgtggtc acgctcatgt
     1201 cgtccttact cccccggcgt ctcggtcgtc gtcgtctccg acgcggcttc ctgcttgatc
     1261 tcttcgagca gacgcgtgtc gccgacctcg cggcgccagt cggtcccctt gagccagccg
     1321 ccgcccgcga cgtcctgcgt gccccagcag cgcgcctcgt gatcgggctt ggcgttcggc
     1381 acggcgatga gcgccggctc cttctccttg cagatgtcgc ggcggtactg gcagcgcggc
     1441 tcgaacttgc agccgggcgg cggcgcgatg aggtccggcg gcagcccttc gatcggcagc
     1501 agcttcgcct tgcgctgctc gtccagtcgc gggatcgaac gcagcaggcc ccaggtgtac
     1561 ggcatcttcg ggttcgcgaa gagctcgccc gtgtcggcct tctcgacgat gcggcccgcg
     1621 tacatcacgt ggatgcgctg cgtcataccc gccacgacgc cgaggtcgtg ggtgatgagg
     1681 atgaacgccg tgcggaactc cctcgcgagg ttcttcagga ggtcgaggat ctgcgcctgg
     1741 atggtcacgt cgagcgcggt cgtcggctcg tccgcgagga tcagcttcgg gctgcacgac
     1801 agggccatcg cgatcatgac gcgctggcgc atgccgcccg agaactggtg cgggtagtcg
     1861 tcaacgcgct tcttggcgct cgggatgccg acgagctcga ccagctcgat ggtgcgcttg
     1921 cgcgcctcgc ccttgtccat cttcagatgg agctcgagcg cctcggagat ctgccggctg
     1981 atcgtgagca cggggttcag gctcgtcatg gggtcctgga agatcatcgc gatgttgttg
     2041 ccgcggatgc cgcggacgtc gtcgtcgtcc atcttgagga cgtcctggcc gtcgaacatg
     2101 atctggccct tcacgatctt gccgggcggc tgcgggatga ggcgcatcag tgagagcgcg
     2161 gtgaccgact tgccgcatcc ggactcgccg acgaggccga gcgtctcacc ggcctcgagg
     2221 ttgaaggtca ccccgtcgac ggcgtgcacg acgccgtccc gcgtgaagaa ctgcgtatgc
     2281 aggtcgttga cctcaagcag cgccatgcga caagctcacc ccttccagaa atcccgcgac
     2341 agtctagcca gacgctagga cttcatccgc gggtccaggg cgtcgcgcag cccgtcgccc
     2401 acgaaggtga aggcgagcat gacggtcgag atgcagagcg ccgggagcat caccgaccac
     2461 ggattcgccg agaaattgat gaatccctcg ttgatcatgg cgccccacga gggcgtcggt
     2521 ggcttgatgc cgaccccgag gaacgacagc gtcgcctcgc cgatgatcgc gcccggcacg
     2581 gcgaacgccg cggtgacgat gagcggcgcc agcgcgttcg ggaacaggtg cttgaacatg
     2641 atgcgccggg acgtcgcgcc catcgagcgc gccgcctcga tgaactcgcg ttccttgagc
     2701 gcgagcacct ggccgcgcgt gagccgtgcg atgccgatcc acccgacgat cgcgacggcg
     2761 gcgaagatga ggacgagccc gccgaggagg ttcccgaacg cggaattgcg gaacgccgcg
     2821 acgatgatca gcacgaagag gaagtcgggg aacgcgtaca cgatgtcggt gacccgcatg
     2881 aggatctgat cgaccgcccc gccgaagtac ccggccgcca ggccgatcgt gacaccgatc
     2941 acgaacacga tcgagacggg gacgaagccc acgaccatgc tgacgcgcga cgcccagatg
     3001 agccgggtca ggatgtcgcg cccgagctgg tccgtcccga ggaggaactc gcgctcggtg
     3061 tacttggacc cggtccagag cggctcgagc tggccgtggc cgctgaactg cttcagcggg
     3121 tcatagggag agatccacgg cgcgaagatc gcgatgaggg cggcggccag gatgacgacg
     3181 agcccggcga tcgcggcctt gttgcggagc agccgctcga gcgcgtcgcg ccagagcgag
     3241 cgctgcttgc gggcgaggac gttgagctgc gccgcgcgct cggacgccgt ggcggtggtg
     3301 gtcgccacgt tatttgccga ccttgatgcg cggatcgagc atcccgtaga cgaggtcgac
     3361 ggagagattg gcgatgacga tgaagaaggc atacagcagc gtcgtcgcca tgatgagcgg
     3421 gtagtcgttc gcgacgatgc cgcggatgta ctcgcggccg atgccgggga cgctgaagat
     3481 cgactcgatg atgaacgagc cggtgatgag gccggccgcc gccggaccga c
//
LOCUS       DASTZR010000056         4264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_708460, whole genome shotgun sequence.
ACCESSION   DASTZR010000056 DASTZR010000000
VERSION     DASTZR010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708460"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VFV20_01225"
     CDS             <1..657
                     /locus_tag="VFV20_01225"
                     /inference="COORDINATES: protein motif:HMM:NF037628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEU5287000.1"
                     /translation="WPAGIVGLAASRLVEDNGRPAVVIAISGAEGKGSCRSISSLHIA
                     EALDECDDLLIKHGGHAMAAGFSLAVERIPAFTDRLDDVVRRRLGGVRPVPSLRVDAE
                     IEPDALTARLALELAELEPCGAGNPRPHLLVRGVRVYGIRQVGADADHLRCKITVGRF
                     TFDAMAFRRGDHAEAMTDAGVVDAVVTVGAGLRGFVELELRDFGPVGTADQMRSMAVA
                     "
     gene            737..2947
                     /locus_tag="VFV20_01230"
     CDS             737..2947
                     /locus_tag="VFV20_01230"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004100248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU5287001.1"
                     /translation="MARANPITARIVGITERLRPRTQGNLDVEAIVKEMLRHYPDGDA
                     ELVRRAAAYAEEAHAGQTRVSGEPYITHPTAVAMLIAELGMDPATIAATLLHDVPEDT
                     AKTSDDIRQQFGDEIGRLVEGVTKLGRLQGQSRDMHQAENVRKMFLAMAVDLRVVVIK
                     LCDRLHNMRTLSPLPIEKQQRIAKQTIEIYAPLAHRLGIWQIKWELEDLAFKYLEPEQ
                     YKELAEQLAARRQVRERYIDQAMKNLASELEKAGIRAELSGRAKHLWSIAQKMRRKGV
                     GFNEVYDLLAIRVIVEDVPSCYAALGVVHTLWPPIPGQFDDYIAVPKANLYQSLHTAV
                     MGPGGQPLEIQVRTREMHGLAEYGIAAHWRYKEGGKGDKSYESKLAWVRQLLEWQHDV
                     ADAREFVESLKVDVFQDEVFVFTPKGEVKALPAGATPIDFAYRIHTDVGHRTIGAKVN
                     GRIVPLDHRLASGDIVEIVTSKAARGPSRDWLSMVQTPGAREKIRQWFKRAERDENIT
                     HGKELLDRELKRLAQRSLPDISDADLRRVTEALNMHDVDTLFASLGYGAITAAQVVTR
                     LGIVDDAQQALPEIAPAIPPTTSRGGVRVKGVGDLLVRFAVCCNPVPGDGIVGYITRG
                     RGVTVHRDDCANVKTSSEKERLVEVEWEHSSARTYPVAIRIEGWDRDGFLRDIAAVIS
                     ENQVQMVALSAASNPDKTATINATLQVTSVEQLSRVLARLESIRDVFSVHRDGR"
     gene            3161..>4264
                     /locus_tag="VFV20_01235"
     CDS             3161..>4264
                     /locus_tag="VFV20_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEU5287002.1"
                     /translation="MRREDIRNIAIIAHVDHGKTTLVDGMLRQSHIFRENQQVAERVM
                     DSNAIERERGITIMAKNTAIEYKGVKINIVDTPGHADFGGEVERVMNMVDGVLLLVDA
                     VDGPMPQTKFVLRQALRRGHRAIVVINKIDRPQARPNHVLNATFDLFLDLGASNEQAE
                     YETVYTNAITGSAGTDPRHMTGTLEPLFEAILATIPPPDVDDAAPTQLLVTTTTYDDY
                     KGKIAVGRLQSGTLRRAQNVARIDHDGTVSRARVTDLFTFQGLAREPVDEVRAGDIVA
                     VAGVADIGIGDTIADPDDPRPLPPIKVEEPTLKMTFAVNTSPFAGREGQHVTSRKLRE
                     RLYAELERDVALRVSDTESPDTLTVSGRGELHLA"
BASE COUNT          723 a         1464 c         1494 g          583 t
ORIGIN      
        1 tggccggctg gcatcgtcgg cctggcggcg tcgcgcctcg tcgaggacaa cgggaggccg
       61 gccgtggtga tcgcgatcag cggcgcggag gggaagggct cctgccgttc gatctcatcg
      121 ctccacatcg ccgaggcgct cgacgagtgc gacgacctgc tgatcaagca cggtggccac
      181 gcgatggcgg cgggcttctc gctggccgtc gagcggatcc ccgcgttcac agaccgactc
      241 gacgacgtcg tccggcggcg gctgggcggg gtccgccccg tgccgtcgct ccgcgtcgac
      301 gccgagatcg agcccgacgc gctcaccgcg cgtctcgcgc tggagctcgc ggagctggag
      361 ccgtgcggcg ctgggaaccc gcggccgcac ctcctggtgc ggggcgtgcg cgtgtacggg
      421 atccgccagg tcggcgccga tgccgaccac cttcggtgca agatcacggt cggccggttc
      481 acgttcgacg cgatggcgtt ccgccgcggc gatcacgccg aggccatgac cgacgcgggg
      541 gtggtggacg cggtcgtgac cgtgggcgcc ggcctgcgcg ggttcgtgga gctggagctt
      601 cgcgacttcg gtccggtcgg caccgccgac cagatgcgca gcatggcggt ggcttgagat
      661 gaagggtcga gcgacgagcg attcactcgc gaggagcgag tccggctcgt cgagtgacaa
      721 cgagacggtg aaataggtgg ctcgagccaa tcccatcacg gcgcgcatcg tcggcatcac
      781 cgagcggctg cgcccgcgca cgcagggcaa cctcgacgtc gaagcgatcg tgaaggagat
      841 gctccgtcac tatccggacg gcgacgccga gctggtgcgc cgggcggcgg cctacgccga
      901 agaggcgcac gcgggtcaga cgcgcgtgtc gggcgagccg tacatcaccc atccgacggc
      961 ggtcgcgatg ctcatcgccg agctcggtat ggatccggcg acgatcgcgg cgacgctgct
     1021 gcacgacgtc ccggaggaca ccgcgaagac gagcgacgac atccggcagc agttcggcga
     1081 cgagatcggc cggctcgtcg agggcgtgac caagctgggc cggctgcagg ggcagtcgcg
     1141 cgacatgcat caggccgaga acgtccgcaa gatgttcctc gcgatggccg tcgaccttcg
     1201 ggtcgtggtc atcaagctct gcgaccggct gcacaacatg cgcaccctgt cgccgctgcc
     1261 gatcgaaaag cagcagcgca tcgccaaaca gacgatcgag atctacgcac cgctggcgca
     1321 ccggctcggg atctggcaga tcaagtggga gctcgaggac ctcgcgttca agtacctcga
     1381 gcccgagcag tacaaggagc tcgccgagca gctggccgcg cggcgccagg tccgcgagcg
     1441 ctacatcgac caggccatga agaatctcgc gtccgagctc gagaaggccg gcatccgcgc
     1501 cgagctctcc ggccgcgcga agcacctctg gtcgatcgcg cagaagatgc gccgcaaggg
     1561 cgtcggcttc aacgaggtct acgacctgct cgcgatccgc gtcatcgtcg aggacgtgcc
     1621 ctcctgctac gcggcgctcg gcgtcgtgca cacgctctgg ccgccgatcc cagggcaatt
     1681 cgacgactac atcgcggtgc caaaggccaa cctgtaccag tccctgcaca ccgcggtgat
     1741 gggcccgggc gggcagccgc tggagatcca ggtgcgcacg cgcgagatgc acgggctcgc
     1801 cgagtacggc atcgccgcgc attggcggta caaggagggc ggcaagggcg acaagagcta
     1861 cgagtcgaag ctcgcgtggg tgcgccagct gctcgagtgg cagcacgacg tcgcggacgc
     1921 ccgcgagttc gtggaatcgc tgaaggtaga cgtcttccag gacgaggtct tcgtgttcac
     1981 gcccaagggc gaggtgaaag cgctccccgc gggagcgacg cccatcgact tcgcgtaccg
     2041 catccacacc gacgtcggcc accggaccat cggggcgaag gtgaacggcc gcatcgtgcc
     2101 gctggaccac cgcctcgcgt ccggagacat cgtcgagatc gtcacgagca aggccgcgcg
     2161 gggtccctcg cgcgactggc tctcgatggt ccagacgccc ggagcgcggg agaagatccg
     2221 gcagtggttc aagcgcgccg agcgcgacga gaacatcacg cacggcaagg agctgctcga
     2281 ccgcgagctg aagcggctcg cgcagcgctc gctgcccgac atcagcgacg ccgacctccg
     2341 gcgggtcacg gaggcgctga acatgcatga cgtcgatacg ctcttcgcct cgctcggtta
     2401 tggtgcgatc acggccgcgc aggtggtgac gcggctgggc atcgtcgacg acgcgcagca
     2461 ggcactgccg gagatcgcgc ccgccatccc gccgacgacc tcgcgcggtg gcgtccgggt
     2521 caagggcgtg ggcgacctgc tcgtgcgttt cgcggtctgc tgcaaccccg tgcccggcga
     2581 cgggatcgtg ggctacatca cgcgcgggcg cggcgtcacc gtgcatcgcg acgactgcgc
     2641 gaacgtgaag acctcgagtg aaaaggagcg gctcgtcgaa gtggaatggg agcacagctc
     2701 cgcacgcacg tatccggtcg cgatccgcat cgaagggtgg gaccgcgacg gcttcctgcg
     2761 tgacatcgcc gcggtgatca gcgagaacca ggtccagatg gtggcgctct ccgccgcgtc
     2821 gaatccggac aagacggcga ccatcaacgc gacgctgcag gtgacgagcg tcgagcagct
     2881 ctcgcgcgtc ctcgcacgtc tcgagagcat ccgcgacgtg ttcagcgtcc accgtgacgg
     2941 tcgctgaggt gacggtcttg tcagctccgc gcggcggcgg cgggtcctgt cccgctccgg
     3001 gcgtcgctct cggctcgcga gtccggctct ccgccgtggc gagacggatc cgtcgcgcag
     3061 caccggggcc cctcgactct cgcttcgccg agagcgatcg ccctgcgcgt gccacccgcc
     3121 gggtcggcgc ggtcctgacg gcttcggaga ccggtctgcg atgagacgcg aggacatccg
     3181 gaacatcgcg atcatcgcgc atgtcgatca cgggaagacg acgctcgtcg acggcatgct
     3241 ccggcagagc cacatcttcc gcgagaacca gcaggtcgcc gagcgcgtga tggacagcaa
     3301 cgccatcgag cgcgagcgcg gcatcacgat catggcgaag aacacggcca tcgagtacaa
     3361 gggtgtgaag atcaacatcg tcgacacgcc cgggcacgcc gacttcggcg gcgaggtcga
     3421 gcgcgtcatg aacatggtgg acggcgtcct gctcctcgtg gacgcggtcg acgggccgat
     3481 gccgcagacg aagttcgtgc tacgccaggc gctccgccgc ggccaccggg ccatcgtggt
     3541 catcaacaag atcgatcggc cccaggcgcg gcccaaccac gtcctcaacg cgacgttcga
     3601 cctgttcctc gatctcggcg cgagcaacga gcaggccgag tacgagacgg tctacacgaa
     3661 cgcgatcacg ggatcggccg ggacggatcc gcggcacatg accggcacgc tcgagccgct
     3721 gttcgaggcg atcctcgcga ccatcccgcc gccggacgtc gacgacgccg cgccgacgca
     3781 gctcctcgtg accaccacga cctacgacga ctacaagggg aagatcgccg tcggccgcct
     3841 gcagagcggg acgctgcgcc gcgcgcagaa cgtggcgcgg atcgatcacg acggcaccgt
     3901 gagccgcgcg cgcgtcaccg acctcttcac gttccagggg ctggcgcggg agcccgtcga
     3961 cgaggtgcgc gccggggaca tcgtggcggt ggccggtgtg gccgacatcg gcatcggcga
     4021 cacgatcgcc gaccccgacg acccgcggcc gcttccgccc atcaaggtcg aggagccgac
     4081 gctgaagatg acgttcgcgg tgaacaccag ccccttcgcc ggacgcgagg gacagcacgt
     4141 caccagtcgc aagctccgcg aacgcctcta cgccgagctg gagcgcgacg tcgcccttcg
     4201 cgtctccgac accgagagcc cggacacgct gacggtcagc ggccgcggcg agctccacct
     4261 cgcg
//
LOCUS       DASTZR010000057         5181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_342502, whole genome shotgun sequence.
ACCESSION   DASTZR010000057 DASTZR010000000
VERSION     DASTZR010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5181
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342502"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..1316
                     /gene="ispG"
                     /locus_tag="VFV20_01240"
                     /gene_synonym="gcpE"
     CDS             114..1316
                     /gene="ispG"
                     /locus_tag="VFV20_01240"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEU5287003.1"
                     /translation="MDAPLNVDLNLPEDRVGPRPRRKSKRVWVGGTAIGDGAPIAVQS
                     MCTTLTHDVDATIRQIERLQEAGCEIARVAVPDKRAGEALPELVRRSILPIVADIHFD
                     YRLALMALDAKVHKLRLNPGNIHNKDGVREVVKKAKEQGTPIRIGVNHGSIGDRQPGE
                     FRDEAQRMVDLAMSEIKILEDLDFDDIVVSVKAFEVPVMVAAYRRLAKLVPYPLHLGV
                     TEAGTALAGSIRSTAGMSVLLYEGIGDTIRVSLSEDPVMEVKAGFDMLKSLGLRDQGL
                     TLVACPSCGRADIDLIPLAKVAEERLRALPTPMKVAVMGCEVNGPGEAKDADVGIAGG
                     VGKGAIFRKGKVVGTYPESELMDALLAEIDKILIEHGKEPYSAKAPKLSHEGQLTPGR
                     SVPAGQRT"
     gene            1403..1879
                     /locus_tag="VFV20_01245"
     CDS             1403..1879
                     /locus_tag="VFV20_01245"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEU5287004.1"
                     /translation="MSIPSIAPPRIAIRHVAPQRYLGKRVHARLDAVGMVVPAAYAEL
                     FAALREAHVTPAGMPFLVASEPSGGEMDIELGVPCADPPARGDLFAGTLPGGRVAVHT
                     FTGRYEDIGPAYASLAEWIRVSGLESAGPPREVHLTGPQDVARPEDHVTEVVWPIV"
     gene            1983..2192
                     /locus_tag="VFV20_01250"
     CDS             1983..2192
                     /locus_tag="VFV20_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287005.1"
                     /translation="MFTRRRILVPAIGAFLAAAFGGGLAGARALLRATRPAPSGTSAT
                     RCAQCGATDHGMLDRRCPLAPKVLS"
     gene            2189..4726
                     /locus_tag="VFV20_01255"
     CDS             2189..4726
                     /locus_tag="VFV20_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU5287006.1"
                     /translation="MTRRRELLRTGATAGVVVAGAVVAPGALGATKGRDYDVPKSLLV
                     DKVVRTTCSPNCTGSCGQLAFVRDGVLVKIQQAADYPDKAYAPRGCMKGLSYLNQVYG
                     PDRITRPLIRTGARGSGEFREASWDEVLDKVASDLARIGATWGWDSIHIFGQVPGSGY
                     IQKGANYRAAALLGMTHGTSFDFNGDLPMGMPITFGVQNAEHEAKDWANSRFLLLVGS
                     NPVETRIPDVHFIFDAVDAGAKLVVVDPVYSPTASKADAWLQIAPGTDAALALALCRQ
                     IVADGAVDVDFMRTYTDAPLLVRTDSGKRLREKDLVAGGSESVFVAWDERAGAPVLAG
                     TARLGFTDGVRPALLGTFQVPLADGTSVGATPGYALVAKELDQWTPERAAAVTAIPAE
                     RIVSLARSFARTKPAAILMGGGSNHWYHGDLTGRAFGLLSALTGNVGRSGGGFSVYVG
                     QYKVRVDTSPWWNPGGQKAKVVPSIYFVRGKTATMSADVPYPKNGWHGLICTFANMFV
                     QAMDVNQLHRTLAGLDLIVVVDHQRTETVQWADVVLPATTWYEKTDLTATPLHPYLQL
                     QQAAIEPVGEARSEVWMWRELMRRIAPDLAAKYFEMTEDDAIRAILQAGGGPGGPTEG
                     ITLEQLREGPVRLRVPDPDVAFASQIRDRAPFPPRSLPAALEATAAFIPTRRIEFYKE
                     DDTFIRLRETVPTYREPFDDGVHDPKTYPLTLLTPHSKWRIHSSYANNEWLAEIHGGR
                     PEVFVHPTDAERRGIATGDVIEVFNTRGSVQAWAHVSDAQRPGAVTLPEGWWPRQFAK
                     GKGVNELTSSAVNPIHEVRFVGNMWSPSTGWKDCRCEIRKAGEAPRA"
     gene            4812..>5181
                     /locus_tag="VFV20_01260"
     CDS             4812..>5181
                     /locus_tag="VFV20_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007140343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU5287007.1"
                     /translation="MVVDQERCVGCWSCAVICKSENDVPLGGWWNRILTDGEALDQPR
                     TDRGQLAMDWLPLACQHCENAPCVKVCPVQATYHRSDGVVMMDNDRCIGCRYCMIACP
                     YGVRTFNWGEAERPTPHETGM"
BASE COUNT          777 a         1835 c         1835 g          734 t
ORIGIN      
        1 cggccagacc gtttggacgt ggcccgcgtc gcggtcgacc gagcgccaga cgatgccttc
       61 gccgggggcg aatcgtctca cgcgcgcaat ggtgcgacac ctacacttga gctgtggacg
      121 cccccctgaa cgtcgacctg aacctgcccg aagaccgcgt cgggccgcgc ccgcgccgca
      181 agtcgaagcg cgtctgggtc ggcgggacgg ccatcggcga cggcgcgccc atcgccgtgc
      241 agtcgatgtg cacgacgctg acgcacgacg tcgacgcgac gatccgccag atcgagcgcc
      301 tgcaggaagc cggctgcgag atcgcgcggg tggccgtgcc ggacaagcga gcgggggagg
      361 ccctcccgga gctcgtgcgc cgctcgatcc ttccgatcgt cgccgacatc cacttcgact
      421 accgcctggc gctcatggcg ctcgacgcga aggtccacaa gctccgcctc aatccgggga
      481 acatccacaa caaggacggc gtgcgcgagg tcgtgaagaa ggcgaaggag cagggcacgc
      541 cgatccgcat cggcgtgaac cacggctcca tcggcgaccg ccagcccggc gagtttcgcg
      601 acgaggcgca gcgcatggtc gacctcgcga tgagcgagat caagatcctc gaggatctcg
      661 acttcgacga catcgtcgtc agcgtgaagg cgttcgaggt ccccgtgatg gtggccgcgt
      721 accgccgtct cgcgaagctc gtgccctatc cgctgcacct cggcgtcacc gaggccggga
      781 ccgcgctcgc gggatcgatc cgctcgaccg ctggcatgag cgtgctcctc tatgagggca
      841 tcggcgacac cattcgcgtg tcgctctcgg aggatccggt gatggaggtg aaggccggct
      901 tcgacatgct gaagtcgctc ggccttcgcg atcagggtct gaccctcgtc gcgtgcccct
      961 cgtgcggacg cgccgacatc gacctcatcc cgctcgcgaa ggtggcggag gagcgcctgc
     1021 gcgccctgcc cacgccgatg aaggtcgcgg tgatgggctg cgaggtgaac ggaccgggcg
     1081 aggccaagga cgccgacgtc ggcatcgccg gcggcgtggg gaagggcgcg atcttccgca
     1141 agggcaaggt cgtcggcacc tatcccgaga gcgagctgat ggacgcgctc ctcgcggaga
     1201 tcgacaagat cctcatcgag cacggcaagg agccgtattc ggcgaaggcg ccgaagctca
     1261 gccatgaggg ccagctcacg ccgggccgga gcgtccccgc ggggcagcgc acctagccgc
     1321 ggcgccacgc gctcgtctgt ggccgcccga cagcgcggcg cggctcagcg cgtcacgctc
     1381 ctcgcgtacg gggaggtgcc ccatgtcgat accttcgatc gctccgccgc gcatcgcgat
     1441 ccgccacgtc gcgccgcagc gctatctcgg gaaacgcgtg cacgcgcgac tcgacgccgt
     1501 cggaatggtc gtccccgccg cgtacgccga gctcttcgcg gccctcaggg aagcacacgt
     1561 cacgccggcg ggcatgccat tcctcgtcgc gtccgagccg agcggcgggg agatggacat
     1621 cgagctcggc gtcccgtgcg ccgacccgcc ggcgcgcggc gacctgttcg ccggaacgtt
     1681 gcccggtggt cgcgtcgcgg tccacacgtt caccggccgg tacgaggaca tcggaccggc
     1741 ctacgcgtcg ctcgcggaat ggatccgcgt gagtggcctc gaaagcgccg gcccgccgcg
     1801 ggaggtccat ctcaccggcc ctcaggatgt cgcgcggcct gaggaccacg tgaccgaggt
     1861 ggtctggccg atcgtctagc gctccgcggc gttggacctg tcggccgtcc ggcgttcccc
     1921 ctcaccgcgc tcggcgccca ccgtctgccg ccggtgccgc cggccacgcg acgcttgtcg
     1981 cggtgttcac acggcggcgc atcctcgtgc cggcgatcgg cgcgttcctg gccgcggcgt
     2041 tcggcggcgg gctggccggg gcgcgggccc tgctgcgtgc gacgcgtccg gcgccatccg
     2101 ggacgagcgc gacgcgctgc gcgcagtgcg gcgcgacgga tcacgggatg ctcgaccggc
     2161 gttgtccgct cgccccgaag gtgctgtcgt gacgcgacgc cgtgagctgc tgcgcacggg
     2221 cgccacggcg ggggtcgtcg tcgccggagc ggtcgtcgcg ccgggcgcgc tcggtgcgac
     2281 caaggggcgc gactacgacg tgccgaagag cctcctcgtg gacaaggtcg tccgaaccac
     2341 gtgctcgccc aactgcaccg ggagctgcgg gcagctcgcg ttcgtacgcg acggcgtcct
     2401 ggtgaagatc cagcaggccg cggactaccc ggacaaggcc tacgcgccgc gcggctgcat
     2461 gaagggcctg tcgtatctga accaggtgta cggtcccgac cgcatcacca ggccgctcat
     2521 ccgcaccgga gcgcgcggct cgggcgagtt ccgcgaagcg tcgtgggacg aggtcctcga
     2581 caaggtcgcc tccgacctcg cgcggatcgg cgcgacatgg ggctgggact cgatccacat
     2641 cttcggccag gtccccggct cgggatacat ccagaagggc gcgaactatc gcgcggccgc
     2701 gctcctcggc atgacgcacg gcacgagctt cgacttcaac ggcgaccttc cgatgggcat
     2761 gccgatcacg ttcggcgtcc agaacgccga gcacgaggcg aaggactggg cgaacagccg
     2821 cttcctgctg ctcgtcggat cgaacccggt cgagacgcgc atccccgacg tccacttcat
     2881 cttcgacgcg gtcgacgccg gcgcgaagct ggtcgtggtc gatcccgtgt attcgccgac
     2941 ggcgtcgaag gccgacgcct ggctccagat cgcgccgggt accgatgcgg ccctcgcact
     3001 cgcgctctgc aggcagatcg tcgccgacgg cgcggtcgac gtcgatttca tgcggacgta
     3061 caccgacgcg ccgctcctgg tccgcacgga cagcggcaag cggctgcgcg agaaagatct
     3121 cgtcgccggc ggctccgagt ccgtgttcgt cgcatgggac gagcgcgccg gggcgcccgt
     3181 gctcgcgggt acggcgcgcc tgggcttcac cgatggcgtg cggcccgcgc tcctcggcac
     3241 gttccaggtc ccgctcgccg atggcacgag cgtcggcgcg acgccggggt acgcgctggt
     3301 cgcgaaggag ctggaccagt ggacgccaga gcgcgccgcc gccgtcacgg cgatacccgc
     3361 cgagcggatc gtctcgctcg cgcgatcgtt cgcgcgcacc aagcccgcgg cgatcctcat
     3421 gggcggcggc tcgaaccact ggtaccacgg cgacctcacc ggtcgcgcct tcgggctcct
     3481 gtccgcgctc accggcaacg tcggccggtc cggcggcggg ttctccgtct acgtcggcca
     3541 gtacaaggtc cgcgtggaca cctcgccgtg gtggaacccc ggcggccaga aggccaaggt
     3601 cgtgccgagc atctacttcg tgcgcgggaa gaccgcgacg atgagcgccg acgtcccgta
     3661 tccgaagaac gggtggcacg gactgatctg cacgttcgcg aacatgttcg tccaggcgat
     3721 ggacgtgaat cagctccatc gcacgctcgc ggggctcgat ctcatcgtcg tcgtggacca
     3781 tcagcgcacc gagacggtgc agtgggcgga cgtcgtgctg cccgcgacca cgtggtatga
     3841 gaagacggac ctcaccgcga cgccgctgca tccatacctc cagctccagc aggcggcgat
     3901 cgagccggtc ggcgaggcgc gctccgaggt ctggatgtgg cgcgagctca tgcgccgcat
     3961 cgcccccgac ctcgccgcga agtacttcga aatgaccgag gacgacgcga tccgcgcgat
     4021 cctgcaggcc ggcggcggcc cgggcggccc gaccgagggg atcacgctcg aacagctgcg
     4081 cgagggcccg gtgcgcctgc gcgtgccgga ccccgacgtc gcgttcgcga gccagatccg
     4141 cgaccgcgcg ccgttcccgc cgcgctcgct gccggccgcg ctcgaggcga ccgcggcgtt
     4201 catcccgacg cggcggatcg agttctacaa ggaggacgac accttcatcc ggctgcgcga
     4261 gacggtgccg acgtaccgcg agccgttcga cgacggcgtg cacgatccaa agacgtaccc
     4321 gctcacgctg ctcaccccgc actccaagtg gcggatccac tcctcgtacg cgaacaacga
     4381 gtggctcgcc gagatccacg gcgggcgccc cgaggtgttc gtgcatccga cggacgccga
     4441 gcggcgcggc atcgccaccg gcgacgtgat cgaggtcttc aatacgcgcg ggtcggtcca
     4501 ggcgtgggcg cacgtctcgg acgcgcagcg gcccggcgcc gtgacgctgc cggagggctg
     4561 gtggccgcgt cagttcgcga agggcaaggg cgtgaacgag ctgacgtcgt cggcggtcaa
     4621 tccgatccac gaggtccggt tcgtcgggaa catgtggtcg ccgagcaccg gatggaagga
     4681 ctgccgttgc gagatccgga aggcggggga ggcgccacgt gcctgagaag gtctcaccgg
     4741 tcgccgcgct cccgccgcgc tcgcgcgcgt acggggctct cggcccgcgc tccgagaagc
     4801 cgcactgggg catggtcgtg gaccaggagc gctgcgtcgg gtgctggtcc tgcgcggtga
     4861 tctgcaaatc cgagaacgac gtgccgctgg gcggatggtg gaaccgcatc ctcaccgacg
     4921 gcgaggcgct cgaccagccg cgcacggacc gcggccagct cgcgatggac tggctgccgc
     4981 tcgcgtgcca gcactgcgag aacgcgccct gcgtgaaggt gtgcccggtc caggccacgt
     5041 atcaccgcag cgacggtgtc gtgatgatgg acaacgatcg ctgcatcggc tgccgctact
     5101 gcatgatcgc gtgcccctat ggcgtgcgga cgttcaactg gggcgaggcc gagcgcccga
     5161 cgccgcacga gacgggcatg g
//
LOCUS       DASTZR010000058         8220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_578557, whole genome shotgun sequence.
ACCESSION   DASTZR010000058 DASTZR010000000
VERSION     DASTZR010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8220
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578557"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="VFV20_01265"
     CDS             complement(<1..892)
                     /locus_tag="VFV20_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEU5287008.1"
                     /translation="MSEVGALVYDWNRDARAAKADYRAVELDDETLRDGLQGPSVRNP
                     PLAVKKRLLHLMDQLGIHSADIGLPGASERARAEIVALAKETTALARLRPNMALRTHP
                     ADVKGAIAAAEEAGVAIEACAFIGSSPIRRYAESWSLDTMLRNVEESVTALVTAGLPV
                     MFVTEDTTRADPETLAKLYETAIGCGARRICASDTVGHATPEGVRNLLTFLRNDVIGD
                     RAVLLDYHGHNDRGLGTVNALTATAIADRVHGCALGIGERVGNTSMDQLLVNMRIWGL
                     IATDLSPLVEYVALVSEHCGI"
     gene            complement(889..2064)
                     /locus_tag="VFV20_01270"
     CDS             complement(889..2064)
                     /locus_tag="VFV20_01270"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5287009.1"
                     /translation="MRAPLIALALLFAACTSVPTVTEPTPRPTREPNTVSVSVLLDLS
                     GSRAPSGQPQRNAMQLWLDRAQAAQPRSAVKLRVKFVDVAGSDAKLLLELRRAAVDDG
                     ADAVVVGVPIALDDTFARAVELASVPVLLTLPAPEPAGAVGGRWTFALAPTPRNIAHA
                     LVDDLAARDLLPPTVLAGDETAPAITERAAFAAEIGARGRVAPTPVILTQPDGPQRAK
                     AAVAGARSVVLSGASEKYAELVRGIPSALEAPRVYLSYLTETADITNLRDQSALVTWP
                     GSRNLATLSVTPFWPERAAFVRAFSDRHGAPSTLAATAYDALAILSAAAEQAPSELEG
                     PRLRLRLETLTFVGVVTRYSFTFTRHAGFAPDDLTYLRWNGRAGAPFLATEPKENPR"
     gene            complement(2070..3098)
                     /locus_tag="VFV20_01275"
     CDS             complement(2070..3098)
                     /locus_tag="VFV20_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU5287010.1"
                     /translation="MAVTSRARHEALGLSLDDVKGMFRAMLMTRMVSERIMQLNRMGR
                     TPFGAGTDGHEAAQIGAAWNIRRGKDWTVPYYRDMGVAFVLGVTPLEEFRMVLAKATD
                     EHSAGRQILNQFSRPRDRIVTRSVCVGTEFPHAVGLALALRNRKEPHIVFAFGGDAST
                     SPGDFHEAANFASVHKLPVVFVIENNLLAISTRIEWQMAAKNVADKAAAYAMPGFIAD
                     GMDVLESYAKTKEAADITRGGGGPTLVELKCYRYQPHTSDDDDTRYRTKDEVREWMAR
                     DPIDHARRYLLAHGTNEDELGAMRTALDAEIEAAITQADREPDPTPETAMRHVYAEDH
                     PLPDGTRA"
     gene            complement(3166..3585)
                     /locus_tag="VFV20_01280"
     CDS             complement(3166..3585)
                     /locus_tag="VFV20_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287011.1"
                     /translation="MPPGLLLAVGYGLLLPVIAILHVRHQRWRESGAILGTIGGASVV
                     VLGIVAAVAPVATIPALFVRGVWWWTIGKMWWETALLPRPLGAATMALALGAFALAAA
                     AAPLALEPAGIASADRAVLGAWAIALSVAVWGSLDAR"
     gene            complement(3681..4562)
                     /locus_tag="VFV20_01285"
     CDS             complement(3681..4562)
                     /locus_tag="VFV20_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011460375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5287012.1"
                     /translation="MAIQKIGVVGFGQMGSGIAQVCAMAGFDVLAREIDQKFIDGGLK
                     RIDGSLARVVKSGRMSEDDAKAARGRIRGTTALADFADRDVVIEAVIEVMDAKKEVWT
                     ELDRVCGPDTLFASNTSSLTIIEMAAATKRADRFAGLHFFNPPVVMQLVEIVRALTTS
                     DATVEALRGFVTKLGKEPVVCKDTPGFIVNRLMVPIMLEAVRALEAGVATVEDIDKAV
                     KLGLRHPMGPFELIDYTGVDINYHVANVFFEEFKDPKWAPPALLKRMFLAGQLGVKTG
                     RGFYEYDESGKKVRAAQ"
     gene            4657..5148
                     /locus_tag="VFV20_01290"
     CDS             4657..5148
                     /locus_tag="VFV20_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017539171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU5287013.1"
                     /translation="MPPFRGPDAVAVALQVNGRRVGVRVRPVRSLLDTLRDDLGLTGT
                     KKVCNEGDCGACTVLIDGRPAYSCITLAIACEGRAVDTIEGISQDGKLHPVQEAFIAH
                     DAYQCGFCTPGQVMSVVGLLRANASPSAEDVKRAIAGNLCRCGAYTNIVAAGVTAAKK
                     AKA"
     gene            5151..7445
                     /locus_tag="VFV20_01295"
     CDS             5151..7445
                     /locus_tag="VFV20_01295"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEU5287014.1"
                     /translation="MPRIRVVKSRDEFEGEIYESLAVVEGSDLPAARAGTELPEIGRA
                     RPRVDGVQRVTGRAMYTQDITLPGMLHAIVLRSPYPRARVRAIDATKALAVQGVHDII
                     HRFNTPKAAFRGEETIFREEVRFVGDEVAAVAAETAGAAYAAIALIEVDYEVLPHVVD
                     LEEAIAPTAPKLEADGNVIEAGKLTRGDPRKAFKEADAVVEATYRTSTQLHNSLETHS
                     AVAQWDGEVLTVWESTQHVFGVRDGLRNALGLPLGKIRVLCDYMGGGFGSKGGVGKYT
                     IMASLLSMHTRRPVRCVLNRTEENLAAGNRSATLQTVKLAGKDGRISAVEHVSWSNAG
                     QAKWVANPTGPTNTLYDLPNLNTKSYRVLTNAGSLSAFRAPGYVEGTFALESAIEELA
                     DAMGVDPLALRRKHAGRPNDPRTGKPYTLNRILDCYTIGAREIGWSKRKAGGTRGATP
                     HRRRGLGMASQIWGGGGGPPAYATVHFNADGSVVLRAGTQDIGTGTRTVLAQICADEL
                     GVEVRNISVRIGDTEGPYGPISAGSLTLASVGPAVRLAAKDAREQFLAAAAGVLETPQ
                     RELRLEAGHVVSKGARTPIAEISKKLENNTVIGKGSRFPNPDNMSIKTFGAHFADVEV
                     DVRTGEIFVLRIVAVHDIGRIVNPLTAASQVEGGVIQALGFALSEERVVDRGLGRVMN
                     ANLEWYKIPTVADVPEIVVRFVDRPDRKANNLSAKGLGEPPIIPTAAAIANAVANATG
                     ARVRHTPMTRARVLEALAIAEVRA"
     gene            7442..>8220
                     /locus_tag="VFV20_01300"
     CDS             7442..>8220
                     /locus_tag="VFV20_01300"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEU5287015.1"
                     /translation="MSYSYTKPRALGEALRLLRSGTAALAGGTDLVMLVRHGLARPER
                     LVDLTGIEGLRGWEHAKGKGLRIGALTPLADLEGSPALAKLLPIVPQALRDAATPQLR
                     NMGTVGGNLLQRNRCWYFRDAAVPCWLKGGERCFAVDGENRYHAVVGAHDCWMVAPSD
                     LAPALIACDAEIEVASSLGQRRMKLADLFVAPRGRERREHAIRPGELITAVRISEKAL
                     ERRGTYLKAMERKAWSFALVSVAASARIKGGKATDARVVLGG"
BASE COUNT         1216 a         2917 c         2930 g         1157 t
ORIGIN      
        1 ggatgccgca gtgctcggac acgagcgcga cgtactccac cagtggcgag agatcggtcg
       61 cgatgagccc ccagatgcgc atgttgacga gcagctggtc catcgaggtg ttgcccacgc
      121 gctcgccgat gccgagggcg cagccgtgga cgcggtcggc gatggccgtc gcggtgagcg
      181 cgttcaccgt cccgagtccg cgatcgttgt ggccgtggta gtcgaggagc accgcgcgat
      241 ccccgatgac gtcgttccgc aggaaggtga gcaggttccg cacgccctcg ggggtggcgt
      301 gaccaacggt gtccgaggcg cagatgcgcc ttgccccgca gccgatcgcc gtctcgtaca
      361 gcttcgcgag cgtctccggg tccgcgcggg tcgtgtcctc agtgacgaac atcaccggca
      421 ggccggccgt gacgagcgcg gtgaccgact cctcgacgtt gcgcagcatg gtgtcgagcg
      481 accaggactc cgcgtaccgc cggatcggtg atgatccgat gaaggcgcat gcctcgatgg
      541 cgacgccggc ctcctccgcc gcggcgatcg cgcccttcac gtccgccgga tgcgtgcgga
      601 gcgccatgtt cggtctgagt cgcgcgaggg cggtcgtctc cttcgccagc gcgacgatct
      661 cggcgcgcgc ccgctccgac gcgcccggca gtccgatgtc ggcgctgtgg atgccgagct
      721 gatccatgag gtgcaggagc cgcttcttca ccgcgagcgg cgggttgcga acgctcgggc
      781 cctgcagccc gtcacgaaga gtctcgtcgt cgagctcgac ggcgcggtaa tcggccttgg
      841 ccgcgcgcgc gtcacgattc cagtcgtaga cgagggcgcc gacctcgctc atcgcgggtt
      901 ctccttgggc tcggtcgcga ggaacggcgc gccggcccgg ccgttccagc ggaggtaggt
      961 gaggtcgtcg ggcgcgaacc ccgcgtgccg cgtgaacgtg aacgagtagc gggtcacgac
     1021 gccgacgaac gtaagagtct cgagccggag ccgcaaacgc ggtccctcaa gctcggaggg
     1081 cgcctgctcc gccgcggcgc tcaggatcgc gagcgcgtcg tatgcggtcg cggcgagcgt
     1141 gctcggtgcg ccgtggcggt cgctgaacgc gcgcacgaac gcggcacgct ccggccagaa
     1201 cggggtcacc gacagcgtcg cgaggttgcg cgagccgggc cacgtcacga gcgcgctctg
     1261 gtcgcgcagg ttcgtgatgt cggcggtctc ggtgagatag gagaggtaca cgcgcggcgc
     1321 ctcgagcgcg ctcgggatgc cgcgcacgag ctcggcgtac ttctcgctcg cgccgctcag
     1381 cacgacgctc ctggcgccgg cgaccgcggc cttggcccgc tgggggccgt ccggctgcgt
     1441 gaggatcacc ggggtcggcg cgacccgacc gcgtgctccg atctcggccg cgaacgccgc
     1501 gcgctccgtg atcgcaggcg ccgtctcgtc acccgcgagc accgtcggcg ggagcaggtc
     1561 gcgcgcggcc aggtcgtcga cgagcgcgtg cgcgatgttc cgcggcgtcg gcgcgagcgc
     1621 gaacgtccag cggccaccca cggcccccgc gggctcgggc gccgggaggg tgagcagcac
     1681 cggtaccgac gcgagctcca cggcacgcgc gaaggtgtcg tcgagcgcga tcggcacgcc
     1741 gaccacgacc gcgtccgcgc catcgtcgac cgcggcgcgg cgcagctcga ggagcagctt
     1801 cgcgtcgctg ccggcgacat cgacgaactt cacgcgcagc ttcaccgcgc tccggggctg
     1861 cgccgcctgt gcgcgatcga gccagagctg catggcgttc cgctgcggct gcccgctcgg
     1921 cgcgcgggat ccggagaggt cgagcagcac cgagaccgaa acggtgttcg gctcgcgggt
     1981 cgggcgcggc gtcggctccg tcacggtggg gacggacgtg cacgcggcga agaggagggc
     2041 gagcgcgatg agcggggcgc gcacgaggac taggcccgtg tgccgtccgg gagcgggtga
     2101 tcctccgcgt agacgtgacg catcgcggtc tccggcgtcg ggtccggttc gcgatcggcc
     2161 tgcgtgatcg ccgcctcgat ctcggcgtcg agcgcggtgc gcatcgcacc gagctcgtcc
     2221 tcgttcgtcc catgcgcgag taggtagcgg cgcgcgtggt cgatcggatc cctcgccatc
     2281 cactcgcgca cctcgtcctt cgtgcggtag cgcgtgtcgt cgtcgtcgga cgtgtgcggc
     2341 tgatagcggt agcacttgag ctcgaccagc gtcggaccgc cgccgccgcg ggtgatgtcg
     2401 gcggcctcct tcgtcttcgc gtacgactcg agcacgtcca tcccgtcggc gatgaatccc
     2461 ggcatcgcgt acgccgccgc cttgtcggcg acgttcttgg ccgccatctg ccattcgatg
     2521 cgggtggaga tcgcgaggag gttgttctcg atcacgaaga ccaccggcag cttgtggacg
     2581 gaagcgaagt tcgcggcctc gtggaagtcg ccgggactcg tcgacgcgtc gccaccgaac
     2641 gcgaacacga tgtgcggctc cttccgattg cgcagcgcga gcgcgaggcc gaccgcgtgc
     2701 gggaactccg tcccgacgca caccgatcgt gtgacgatgc ggtcacgcgg cctcgagaac
     2761 tggttcagga tctgccgtcc ggccgagtgc tcgtccgtcg ccttcgcgag caccatgcgg
     2821 aactcctcga gcggcgtgac gccgagcacg aacgcgacgc ccatgtcgcg gtagtagggg
     2881 accgtccagt ccttgccgcg gcggatgttc caggcggcac cgatctgcgc ggcctcgtga
     2941 ccgtcggtcc cggcgccgaa gggcgtgcgg cccatgcggt tgagctgcat gatccgctcg
     3001 ctgaccatcc gcgtcatgag catcgcgcgg aacatgccct tcacgtcgtc gagcgagagc
     3061 ccgagcgcct cgtgacgggc gcgagacgtc acggccatgc gctgatggta ggagttagtc
     3121 gtccccgtcg tcgtgttcgt cggcggtcgc gcacgcaaac gaccgtcaac gcgcgtcgag
     3181 cgacccccac accgcgaccg acagcgcgat ggcccacgcg ccgagcaccg cgcggtccgc
     3241 ggaggcgatg ccggccggct cgagcgcgag gggcgccgcg gcggccgcga gcgcgaacgc
     3301 gccgagcgcg agcgccatcg tcgcggcacc gagcggccgc ggcaggagcg cggtctccca
     3361 ccacatcttc ccgatcgtcc accaccacac gccgcggacg aacagcgccg ggatggtcgc
     3421 gaccggtgcg acggccgcga cgatgccgag gacgacgaca gacgcgccgc cgatcgtgcc
     3481 gaggatcgct ccgctctcgc gccatcgctg atggcgcacg tgcaggatcg cgatgaccgg
     3541 gagcaggaga ccgtagccga ccgcgagcag caggccgggc ggcacggggc cgaacgcgat
     3601 cgacgcgatc tgcgcgacgc cggcgagcgc tccccacagc gcggccttcg cgttgtcggc
     3661 gcggacgagc tcgggcggat ctactgcgcc gcgcgtacct tcttcccgct ctcgtcgtac
     3721 tcgtagaagc cgcgccctgt cttcacgccg agctgacccg cgaggaacat ccgcttgagg
     3781 agcgcgggcg gcgcccactt cggatccttg aactcctcga agaacacgtt cgccacgtgg
     3841 tagttgatgt cgacgcccgt gtagtcgatg agctcgaacg gtcccatcgg gtggcgcaga
     3901 ccgagcttca cggccttgtc gatgtcctcc accgtcgcga cgcccgcctc cagcgcgcgc
     3961 acggcctcga gcatgatcgg caccatcagc cggttcacga tgaagccggg cgtgtccttg
     4021 cagacgaccg gctccttccc gagcttcgtc acgaagccgc gcagcgcctc gaccgtcgcg
     4081 tcgctggtgg tgagcgcgcg cacgatctcg acgagctgca tgacaacggg cgggttgaag
     4141 aagtggaggc ccgcgaagcg gtccgcgcgc ttcgtcgcgg cggccatctc gatgatcgtg
     4201 aggctcgagg tgttcgaggc gaacagcgtg tccggcccgc agacgcgatc gagctcggtc
     4261 cagacctcct tcttcgcgtc catcacctcg atgacggcct cgatgaccac gtcgcggtcc
     4321 gcgaagtccg cgagggccgt cgtgccccgg atgcggccgc gcgcggcctt cgcgtcgtcc
     4381 tcgctcatac ggccggactt caccacgcgc gcgagcgagc cgtcgatgcg cttcagcccg
     4441 ccgtcgatga acttctgatc gatctcgcgc gccagcacgt cgaagccggc catcgcacag
     4501 acctgcgcga tccccgagcc catctggccg aagcccacga cgccgatctt ctggatggcc
     4561 atctggaacg gacctccccg tggtgtcgtc cgcagcatac ggtcccgtaa cgatcgtccg
     4621 acggcgtcgc ctgagcggcc tcggtacgat ggagacgtgc ccccattccg cggaccggac
     4681 gccgtcgccg tcgcgctcca ggtcaacggc cgccgcgtcg gcgtccgcgt ccggccggtg
     4741 cgctcgctgc tggacacgct ccgcgacgac ctcgggctca ccggcacgaa gaaggtctgc
     4801 aacgagggcg actgcggcgc ctgcaccgtg ctcatcgacg gccgcccggc gtactcgtgc
     4861 atcaccctcg cgatcgcctg cgaggggcgc gcggtcgaca cgatcgaggg catctcccag
     4921 gacgggaagc tgcatccggt gcaggaggca ttcatcgcgc acgacgccta tcagtgcggc
     4981 ttctgcacgc cgggccaggt catgtcggtc gtcggcctgc tccgcgcgaa cgcttcgccg
     5041 agcgcggagg acgtgaagcg cgcgatcgcc ggcaacctct gccggtgcgg cgcgtacacg
     5101 aacatcgtcg ccgccggtgt caccgcagcg aagaaggcga aggcctagcc atgccgcgta
     5161 tccgtgtcgt gaagagccgc gacgagttcg agggcgagat ctacgagagc ctcgccgtcg
     5221 tcgaggggtc cgatctcccc gcggcccgtg cgggcaccga actcccggag atcggccgcg
     5281 cgcgcccgcg cgtcgacggt gtccagcgtg tcaccggccg cgcgatgtac acccaggaca
     5341 tcaccctgcc ggggatgctg cacgcgatcg tgctgcgctc tccgtacccg cgagcgcggg
     5401 tccgcgcgat cgacgcgacg aaggcgctcg ccgtgcaggg ggtgcacgac atcatccacc
     5461 gcttcaacac gccgaaggcg gcgttccgcg gcgaggagac gatcttccgc gaagaggtcc
     5521 gcttcgtcgg cgacgaggtc gcggcggtcg ccgccgagac cgccggtgcg gcgtacgcgg
     5581 cgatcgcgct catcgaggtg gactacgagg tgctgccgca cgtcgtcgac ctggaggagg
     5641 cgatcgcgcc gacggcgccg aagctcgagg ccgacggcaa cgtgatcgaa gcggggaagc
     5701 tcacgcgcgg cgatccgcga aaggccttca aggaggccga cgccgtcgtc gaggcgacgt
     5761 accgcacgtc gacgcagctg cacaactcgc tcgagacgca cagcgccgtc gcgcagtggg
     5821 acggtgaagt gctcaccgtc tgggaatcga cgcagcacgt cttcggcgtg cgcgacgggc
     5881 tgcggaacgc cctcggcctg ccgctcggga agatccgcgt cctctgcgac tacatgggcg
     5941 gcggcttcgg ctcgaagggt ggcgtgggca agtacacgat catggcgtcg ctcctctcga
     6001 tgcacacgcg ccgtccggtg cgctgcgtgc tgaaccgcac cgaggagaac ctcgccgcgg
     6061 gcaaccgctc ggcgacgttg cagaccgtga agctcgcggg gaaggacggc cgcatcagcg
     6121 ccgtggagca cgtgtcctgg tcgaacgcgg gtcaggcgaa gtgggtcgcg aacccgacgg
     6181 ggccgacgaa cacgctgtac gaccttccga acctcaacac gaagtcgtac cgcgtcctga
     6241 cgaacgccgg ctcgctgtcg gcgttccgcg cgccgggcta cgtggagggc acgttcgcgc
     6301 tcgagagcgc gatcgaagag ctcgccgatg ccatgggagt ggacccgctc gccctccgac
     6361 gcaagcacgc tggaagaccg aacgacccgc gcaccggcaa gccgtacacg ctcaaccgga
     6421 tcctcgattg ctacacgatc ggtgcgcgcg agatcggctg gtcgaagcgc aaggccggcg
     6481 gcacgcgcgg cgcgacgccg caccggcgcc gcgggctcgg catggcctcg cagatctggg
     6541 gcggcggcgg cgggccgccg gcgtacgcga ccgtgcattt caacgcggac ggcagcgtcg
     6601 tgctgcgtgc gggcacgcag gacatcggca ccggcacgcg aaccgtgctt gcgcagatct
     6661 gcgccgacga gctcggcgtc gaggtgcgca acatctcggt gcgcatcggc gacaccgagg
     6721 gcccctacgg tccgatcagc gcgggatccc tcacgctcgc gtccgtcgga ccggcggtcc
     6781 gcctcgcggc gaaggacgcg cgcgagcagt tcctggccgc cgcggccggc gtgctcgaga
     6841 cgccacagcg cgagctccgc ctcgaggccg gccacgtggt gagcaagggc gcgcgcacgc
     6901 cgatcgccga gatctccaag aagctcgaga acaacaccgt catcgggaag ggatcgcgct
     6961 tcccgaatcc ggacaacatg tcgatcaaga cgttcggcgc gcacttcgcc gacgtcgagg
     7021 tggacgttcg gaccggcgag atcttcgtgc tgcgcatcgt cgccgtgcac gacatcggcc
     7081 gcatcgtgaa cccgctcacg gcggcgtcgc aggtcgaggg cggcgtgatc caggcgctcg
     7141 gcttcgcgct ctccgaggag cgcgtcgtcg accgcggcct cggccgggtc atgaacgcga
     7201 acctggagtg gtacaagatc ccgaccgtcg cggacgtgcc cgagatcgtc gtgcgcttcg
     7261 tcgaccggcc ggaccgaaag gcgaacaacc tcagcgcgaa gggcctcggc gagccgccga
     7321 tcatccccac cgcggccgcg atcgcgaacg cggtggccaa cgcgaccggc gcgcgcgtgc
     7381 gccacacacc gatgacgcga gcgcgcgtgc tcgaggccct cgccatcgcg gaggtgcgcg
     7441 cgtgagctac tcgtacacga agccccgcgc gctcggcgag gcgctccgcc ttctgcgcag
     7501 cggcacggcc gccctcgccg gcggcaccga cctcgtgatg ctcgtgcgac acggcctcgc
     7561 gcgccccgag cggctcgtgg atctcacggg gatcgagggc ctgcgcggct gggagcacgc
     7621 aaagggcaag ggcctgcgca tcggcgcgct cacgccgctc gcggacctcg aaggttcacc
     7681 cgcgctcgcg aagctcctgc ccatcgtccc gcaggcgctg cgcgacgcgg ccacgccgca
     7741 gctgcgcaac atgggcaccg tcggcggcaa cctgctgcag cgcaaccgct gctggtactt
     7801 ccgcgacgcc gcggtgccgt gctggctcaa gggcggcgag cgctgcttcg cggtggatgg
     7861 cgagaaccgc taccacgccg tcgtcggcgc gcacgactgc tggatggtgg cgccgtcgga
     7921 cctcgcgccg gcgctcatcg cgtgcgacgc ggagatcgag gtcgcctcca gcctcggcca
     7981 gcggcgcatg aagctcgccg acctcttcgt cgcgccgcgc ggccgcgagc ggcgcgagca
     8041 tgcgatccgt cccggcgagc tcatcaccgc cgtccggatc tcggagaagg cgctcgagcg
     8101 ccgcggcacc tacctcaagg cgatggagcg caaggcctgg agcttcgcgc tcgtgtcggt
     8161 cgcggcgagc gcgcgcatca agggcggcaa ggcgacggac gcacgcgtcg tgctcggcgg
//
LOCUS       DASTZR010000059         5550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_81761, whole genome shotgun sequence.
ACCESSION   DASTZR010000059 DASTZR010000000
VERSION     DASTZR010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5550
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81761"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..610
                     /gene="smpB"
                     /locus_tag="VFV20_01305"
     CDS             104..610
                     /gene="smpB"
                     /locus_tag="VFV20_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004011905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEU5287016.1"
                     /translation="MRERARSTPEAPEKKSRDVDIADNRRAFYDYSIEERLEAGIALT
                     GTEIKSLRAGHVNMRDGFVRIDAGQAWLRGVHISPWTHTGYDNHEPLRPRKLLLHRRE
                     IAFLERGAAAKGYTIVPLRLYTKHGRAKIELGLARGKRRYEKRQVIKEREATREMDAA
                     VRRRVPRT"
     gene            complement(612..2228)
                     /locus_tag="VFV20_01310"
     CDS             complement(612..2228)
                     /locus_tag="VFV20_01310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEU5287017.1"
                     /translation="MSGEMTRNAKIFATVGVGLALFLAALDQTIVGTALPRIVAELNG
                     LEYYAWVATAYMVSSTTLTPIAGKLGDLFGRKPFLLGGMIGFVAASALCGQAQDMTEL
                     VLFRGIQGIFGGVLFASVFATLADLFPPHVRARMQGIFGGIFGIASIVGPTIGGYLTD
                     NVGWRWVFYVNVPVGVLAVLVVLFTLPRTKHESSWRDVDFVGAVLLAVAIVPMLVAFS
                     ITHDHDILSPEVLGLLGFSAVAWVIFYFVERREAHPMVPFDLWKNPTFAVSSIVGFFV
                     AFGMFGAILYVNLIYQGVLGIPATNSGLLVTPLMVGMIVSSIVTGQLMVRIDRYRYLG
                     TVGISVMALGLWLLSQVTVGTPEIDVVRDLVFVGIGMGVAMPLYVNAVQSALPRQYLG
                     VASSQIQFWRNIGSTVGVAILGAVLSHELPVKIAEKVSALNLPPQFASAIPQGGSAQA
                     IFDPNQIAATRAALPPQALPLFDQVLTAVRSALAATTHDVFVYSAAIVVVAVVASLFL
                     KEVPLRAHTPREVQEVRDAESREEIPAFGK"
     gene            complement(2218..2709)
                     /locus_tag="VFV20_01315"
     CDS             complement(2218..2709)
                     /locus_tag="VFV20_01315"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU5287018.1"
                     /translation="MALATGLFGVMHLFKAVAADAAQACDVGSLERAGMLFRLKGGPC
                     RAGRLAQHARLSPSAITEIVEGLERDGLVRREADAEDRRAVRVGLTADGRRHVQRFEH
                     AAAVALAERLASLTSAQRQRLRVAFNDIREVVGTTDFTSSSHARPTLRHTTRGHKETA
                     NVR"
     gene            2843..3700
                     /locus_tag="VFV20_01320"
     CDS             2843..3700
                     /locus_tag="VFV20_01320"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU5287019.1"
                     /translation="MQYLAFIGCCLIWGSTFLAIRIGNQALPPEWAATLRLSLAAPLL
                     ALVVVIRRDRWPRGPALEAAVLFGVFNFGVNLALLYWGERVVPSGIAAVLYATVPLST
                     ALIAALLRVERLRARKLIAALVAIGGVATIFAGEMRFDVPLDGLLAVLLAATAASLSS
                     VFLKRAPQPSVFSTNAVGAAAGALICLAASIVLGEDRTLPSTVAAWWPVIYLTLAGSL
                     GAYVLYTWLVGHWSVTNASYVGVVVPVIAVALGAVVLQETRAPETYLGAAIVIAAVVV
                     ALRGGGAAH"
     gene            3748..4368
                     /locus_tag="VFV20_01325"
     CDS             3748..4368
                     /locus_tag="VFV20_01325"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEU5287020.1"
                     /translation="MRVRLDADVRTKDGHRAGAVKRVVFDPAKNEVSAYVVSTGGLLG
                     HDVIVSPEVLERASADGDEVVLDMTKSELDGLERYEEKAYAPPPSGWLAPASYAFPSA
                     AYLFPLEAGVPLAAPEPEERRAHRPTITEGMKVKDATGLVIGTVREVRVDDMTGELRS
                     VVVRDDSPLASDEDATEIPADHIDIGDDELHVIEQGGARATKRDGV"
     gene            4452..4880
                     /locus_tag="VFV20_01330"
     CDS             4452..4880
                     /locus_tag="VFV20_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287021.1"
                     /translation="MTGERLPAPIAARVVEVVQDRRSVAGLRSDVDHIRAIAAAPPTR
                     FLRRVVFMWFRVFVHEVKHGKVERVNVRIPIPIPLVGVFLAPGLSRAKALAALALAES
                     SDQPAQAVSDYLDSVMGFEFVRVDERKGNDNHQLVVVGFD"
     gene            complement(4877..5368)
                     /locus_tag="VFV20_01335"
     CDS             complement(4877..5368)
                     /locus_tag="VFV20_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEU5287022.1"
                     /translation="MTRLRVVQGDIATLDVDAVVNAANDHLWMGAGVAGALKRAGGDE
                     IEREAMAKGPIAVGEAVVTGAGRLRARHVIHAAVMGQDLRTSADAIARATTSALERAE
                     EIGARSVGLPAFGTGVGGFPLRECARIMVAAVRAHDGSVDEVVFALRGDDAVRAFEEA
                     LGA"
BASE COUNT          868 a         1944 c         1928 g          810 t
ORIGIN      
        1 ctcttctttt tttgcgtcgt tcgttccggg tcgcgcacgc ggcagcggct cggcggcgct
       61 cagccttttc gagcgacgcg gcggcggcga ctacgctttc accatgcgcg agcgagcccg
      121 cagcaccccg gaggcgcccg agaagaagag ccgcgatgtg gacatcgcgg acaaccggcg
      181 cgccttctac gactactcca tcgaggagcg gctcgaggcg gggatcgccc tcaccggcac
      241 cgagatcaag tcgctccgcg ccggacacgt gaacatgcgc gacggtttcg tgaggatcga
      301 cgccggccag gcctggctgc gcggggtcca catctcgccc tggacgcaca ccggctacga
      361 caaccacgag ccgctgcggc cgcggaagct gctgctccat cgacgcgaga tcgcgttcct
      421 cgagcgcggc gccgccgcga agggctacac catcgttccg ctccgcctgt acacgaagca
      481 cggccgcgcc aagatcgagc tcgggcttgc ccgcggcaag cgccgctacg agaagcggca
      541 ggtcatcaag gaacgcgagg ccacgcgcga gatggacgcg gcggtacgcc gccgcgtccc
      601 tcgcacatag ctcacttccc gaacgcgggg atctcctcgc gagactccgc gtcgcggact
      661 tcctggacct cgcgcggcgt gtgcgcgcgc agcgggacct ccttcaggaa cagcgacgcg
      721 acgacggcga ccacgacgat cgcagcggaa tacacgaaga cgtcgtgcgt cgtcgccgcc
      781 agagcggacc gcaccgccgt cagtacctgg tcgaacagcg gcagggcctg cggcggcagc
      841 gccgcgcgcg tcgccgcgat ctggttcgga tcgaagatcg cctgcgcgct gccgccctgc
      901 gggatcgcgc tcgcgaactg cggcgggagg ttcagcgcgg acaccttctc ggcgatcttc
      961 accggcagct cgtgggagag caccgcaccg aggatcgcga cgcccaccgt gctgccgatg
     1021 ttgcgccaga actggatctg gctcgacgcc acaccgaggt actggcgcgg tagcgcgctc
     1081 tggacggcgt tcacgtagag cggcatggcg acgcccatgc cgatgccgac gaacaccagg
     1141 tcgcgcacga cgtcgatctc cggcgtgccc acggtgacct gtgacagcag ccagaggccg
     1201 agcgccatga cgctgatgcc gaccgtaccg agatagcggt agcggtcgat gcggaccatg
     1261 agctgcccgg tgacgatgct cgagacgatc atgccgacca tgagcggcgt cacgagcagg
     1321 ccggagttcg tcgcggggat gcccagcacg ccctggtaga tgaggttcac gtagaggatc
     1381 gcgccgaaca tgccgaacgc gacgaagaag ccgacgatgg acgagaccgc gaaggtcgga
     1441 ttcttccaaa ggtcgaacgg caccatcggg tgcgcctcgc gccgctcgac gaagtagaag
     1501 atcacccagg cgacggcgga gaacccgagc agcccgagca cctcgggcga gaggatgtcg
     1561 tggtcgtgcg tgatcgagaa ggccacgagc atgggcacga tcgcgacggc gagcagcacg
     1621 gcgccgacga agtccacgtc gcgccacgag gactcgtgct tcgtccgcgg cagcgtgaag
     1681 aggacgacga gcaccgccag gacgccgacg gggacgttca cgtagaagac ccagcgccag
     1741 ccgacgttgt cggtcaggta accgccgatg gtgggcccga cgatcgacgc gatgccgaag
     1801 atgccaccga agatgccctg catgcgcgca cggacgtgcg gcgggaagag gtccgcgagg
     1861 gtggcgaaga cgctcgcgaa gagcacgcca ccgaagatgc cctggatgcc gcggaacaga
     1921 acgagctcgg tcatgtcctg cgcctggccg cagagcgccg aggcggcgac gaagccgatc
     1981 atcccgccga gcaggaaggg cttgcggccg aagaggtcgc cgagcttgcc cgcgatcggc
     2041 gtcagcgtgg tcgacgagac catgtaggcg gtcgcgaccc aggcgtagta ctcgagcccg
     2101 ttcagctcgg ccacgatgcg cggcagcgcc gtgccgacga tcgtctggtc gagggcggcg
     2161 aggaagagcg cgagcccgac ccccaccgtg gcgaagatct tcgcgttccg cgtcatctca
     2221 ccggacattg gcagtctcct tgtggcctct ggtggtgtgg cgcagcgtgg gtctcgcgtg
     2281 tgaagaactc gtgaagtccg tcgtccccac gacctcacgg atgtcgttga acgcgacgcg
     2341 cagccgctgc cgctgcgcgc tggtgagtga cgcgagccgc tcggccagcg cgaccgcggc
     2401 agcatgctcg aaccgctgca cgtgtcgacg cccatcggcg gtgagtccga cgcggacggc
     2461 gcggcggtcc tccgcgtccg cctcacgtcg caccagaccg tcgcgctcca gtccctcgac
     2521 gatctcggtg atcgccgacg gcgagagcct cgcgtgctgc gccaaccggc ccgcgcggca
     2581 cggcccaccc ttcagtcgga agagcatccc ggcgcgctcg agcgagccga cgtcgcaggc
     2641 ctgcgcggcg tccgcggcga cggccttgaa gaggtgcatc acgccgaaga ggccggtggc
     2701 cagggccatc tcttcatctg agggtgtacg ggtcgacgac acgtgtgccg attcctttcg
     2761 gtaggtgaat agttcgggaa ccgaaagagt agcgggaacg acgtagcgcg ggcaaggggg
     2821 cgctccgtat cgtgcgcaac tgatgcagta cctcgcattc atcggttgct gcctcatctg
     2881 gggcagcacc ttcctcgcca tccggatcgg caaccaggcg ctgccacccg agtgggccgc
     2941 gacactgcgg ctgtcgctcg cggcgcccct cctcgcgctc gtcgtggtca tccgacgaga
     3001 tcgctggccg cgcggccccg cgctcgaggc cgcggtgctg ttcggcgtct tcaacttcgg
     3061 cgtgaatctc gccctcctgt attgggggga acgcgtggtg ccgagcggca tcgccgccgt
     3121 gctctacgcg acggtgccgc tctccaccgc gctcatcgcc gcgctgctcc gcgtcgagcg
     3181 gctgcgggcc cgcaagctca tcgcggccct cgtcgcgatc gggggcgtgg cgacgatctt
     3241 cgcgggcgag atgcgcttcg acgttccgct ggacggcctc ctggccgtgc tgctcgccgc
     3301 caccgcggcg tccctgtcga gcgtcttcct caagcgcgcg ccgcagccct cggtgttctc
     3361 cacgaacgcg gtcggcgccg ccgcgggcgc gctcatctgc ctcgccgcga gcatcgtcct
     3421 cggcgaggac cggacgctgc catcgaccgt ggccgcctgg tggccggtga tctacctcac
     3481 gctcgctggg tcgctcggtg cctatgtcct ctacacgtgg ctcgtcggcc attggtcggt
     3541 gacgaatgcc agctacgtgg gtgtggtcgt cccggtcatc gcggtcgcgc tcggcgcggt
     3601 cgtgctccag gagacgcgtg ccccggagac gtacctcggc gccgccatcg tgatcgccgc
     3661 tgtggtcgtc gccctgcgcg gaggcggcgc cgcgcactga gtggcacctg ccatgcgggg
     3721 gctggctccg cgatcggagg tgcggcgatg cgagtgagac tcgatgcgga cgtacggacc
     3781 aaggacgggc accgcgcggg agcggtgaaa cgcgtcgtgt tcgatccggc aaagaacgag
     3841 gtgagcgcgt acgtcgtgag caccggaggg ctcctcggac acgacgtgat cgtgtcgcct
     3901 gaggtcctcg agcgagcgtc ggccgacggt gacgaggtcg tgctcgacat gacgaagtcc
     3961 gagctcgacg gtctggagcg ctacgaggag aaggcgtacg cgccaccgcc gtcggggtgg
     4021 ctcgcgccgg cgtcgtacgc gttcccgagc gcggcgtatc tcttcccgct cgaggcgggc
     4081 gtcccgctcg ccgcgccgga gccagaggaa cgacgtgccc accgcccgac gatcaccgag
     4141 ggcatgaagg tgaaggacgc gaccggactc gtgatcggca ccgtgcgcga ggtgcgcgtg
     4201 gacgacatga ccggcgagct gcgttcggtc gtcgtgcgcg acgacagtcc gctcgcgagc
     4261 gacgaggacg cgacggagat ccccgccgat cacatcgaca tcggcgacga cgagctccac
     4321 gtcatcgaac agggcggcgc tcgcgcgacg aagcgagacg gcgtctagcc cgtcttcgcc
     4381 gcgcgccgtc gccccggtcg ttcacgcgga acgtatccgg tgatgctttt cccggctcgt
     4441 atgctccgcg ggtgaccggc gaacgcctgc ccgcccccat cgccgcacgg gtcgtcgagg
     4501 tcgtccagga ccggcgctcc gtggccggcc tgcggagtga cgtcgaccac attcgcgcca
     4561 tcgccgcggc gccgcccacg cgcttcctcc ggcgcgtcgt gttcatgtgg ttccgcgtct
     4621 tcgtccacga ggtgaagcac gggaaggtgg agcgtgtgaa cgtgcggatc cccatcccga
     4681 tcccgctcgt cggcgtgttc ctcgcgccgg gcctgtcgag ggcgaaggcg ctcgccgcgc
     4741 tcgcgctcgc cgagagctcg gaccagccgg cgcaggcggt gagcgattat ctcgactcgg
     4801 tcatgggctt cgagttcgtg cgcgtcgacg agcgcaaagg caacgacaac caccagctcg
     4861 tcgtcgtggg attcgactag gccccgagcg cctcctcgaa ggcgcggacc gcgtcgtcgc
     4921 cacgcagcgc gaagaccacc tcgtccaccg acccgtcatg cgcgcgcacg gccgcgacca
     4981 tgatccgtgc gcactcgcgc agcgggaacc cacccacacc cgtcccgaac gccggcagcc
     5041 ccacgctgcg ggcaccgatc tcctccgcgc gctccagcgc gctcgtcgtc gcgcgcgcga
     5101 tcgcgtccgc actcgtgcgc agatcctggc ccatcaccgc cgcgtgtatg acgtgacgcg
     5161 ccctgagccg tccggcgccg gtgaccacgg cctcacccac cgcgatcggc cctttggcca
     5221 tcgcctcgcg ctcgatctcg tcgccgccgg cgcgcttcag cgcaccggcc accccggccc
     5281 ccatccagag gtggtcgttc gccgcgttga caacggcgtc gacgtcgagc gtcgcgatgt
     5341 ctccctgcac gacgcggagt cgtgtcatga gacggctcgg agcgtggatc cggcgaaggc
     5401 acaccgcacc acgggcggtg ggacggctcg agagcctcgc gcacgtgtcc tcgtcacgtc
     5461 tgaaaggatg acggacaagg cggcggcccc cggtggggcc gccattcctg tggagatggg
     5521 ggagggtcga actccccgtc caaggccatt
//
LOCUS       DASTZR010000060         2823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_421921, whole genome shotgun sequence.
ACCESSION   DASTZR010000060 DASTZR010000000
VERSION     DASTZR010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421921"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..1171
                     /locus_tag="VFV20_01340"
     CDS             5..1171
                     /locus_tag="VFV20_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEU5287023.1"
                     /translation="MTQTKVDAVPALTALTEEEQLFQSSVYEFADKEIRPLVREMDEH
                     AKIPRTLVDQLFELGVMGIEVPEAYGGAGGRFFHSVLAVEELSRVDPSIGVLVDVQNT
                     LVINAVLRWGNDDVKRRYLPKLASSIVGAYALSEAGSGSDAFALTTRAREDGDAFVVS
                     GRKLWITNGNEADVFIVFATINPEAGYRGITAFMVDRGTAGFTVGKKEDKLGIRASST
                     CELILEDCRIAKASVLGEVGKGYKVAIETLNEGRIGIGAQMIGLAKGALEHSVRYTKE
                     RKQFGKPIAEFQAVQHQLARAAVELEAAKLTVYNAARLRDAGRPFLTEAAICKILSSE
                     VAERVSSLAVNLFGGNGFVKEYPVEKLFRDAKIGQIYEGTSNLQLQTIAKQLLG"
     gene            complement(1175..1807)
                     /locus_tag="VFV20_01345"
     CDS             complement(1175..1807)
                     /locus_tag="VFV20_01345"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEU5287024.1"
                     /translation="MEHTTYASRLRYLDAEDVDATVVDYDGLDVCGPDGTRIGDLDGF
                     LVDAEACRVRYVVVDSGGWFRSRRVLIPVGHATIGSERKTLRVEVTRDALSRYPPFDE
                     DRFREFSDEDMRLFERSIRDACCPDKAPGDEAAAYDTHPHFAQPEWWVSGSYPQERLL
                     PVETGVYRSGTIRERFGAPPQPVEASDAPRPVRTAQRILFDDEEPRQSER"
     gene            complement(1894..>2823)
                     /locus_tag="VFV20_01350"
     CDS             complement(1894..>2823)
                     /locus_tag="VFV20_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5287025.1"
                     /translation="KASAVVAVIAAVNNSLVTEPIERFGTDAQKQAWLRRLATGESLG
                     AFALSEEHAGSDAANQHTVARLDERGYVLNGKKVWVANAEAAELAIVFAATHPSTRFA
                     RSGQALGRRITAFLVPMDTPGIRRVMTADSLGVRGLGCMDLELVNVRVDADALLGAPG
                     DGFKIAMWALDGGRVAIAAQALGVGQAALEEALEYAKTREAFGQPIGNYQAIQWMLAD
                     MATELDAARMLTLKAASLVPGDVTLEAAMAKLYASEAAHRAADKAMQILASKGYRRGS
                     VAERLFRDVRATEIYQGTSEVQRMVIAEHLLKP"
BASE COUNT          471 a          929 c          987 g          436 t
ORIGIN      
        1 agtcgtgacc cagacgaaag ttgatgccgt acctgcgttg acggcgctga ccgaggaaga
       61 gcagctcttc cagagcagcg tgtacgagtt cgccgacaag gaaatccgcc cgctcgtccg
      121 cgaaatggac gagcacgcca agatcccccg gacgctcgtg gaccagctct tcgagctcgg
      181 cgtcatgggc atcgaggtcc cggaagccta cggcggcgcc gggggccgct tcttccactc
      241 ggtcctcgcc gtggaggagc tctcgcgggt cgatccttcc atcggcgtcc tcgtggacgt
      301 ccagaacacc ctcgtcatca acgcggtgct tcgctggggc aacgacgacg tgaagcggcg
      361 ctacctgccg aagctggcct cctccatcgt gggcgcgtac gcgctgtcgg aggcgggatc
      421 cggcagcgac gcgttcgcgc tcaccacccg cgcccgcgag gacggcgacg cgttcgtcgt
      481 cagcggccgc aagctgtgga tcaccaacgg caacgaagcg gacgtcttca tcgtgttcgc
      541 gaccatcaac ccggaggccg gataccgcgg catcaccgcc ttcatggtcg atcgcggcac
      601 cgcgggattc acggtcggga agaaggagga caagctcggc atccgcgcca gcagcacgtg
      661 cgaattgatt ctggaggact gccgcatcgc gaaggccagc gtcctgggcg aggtcgggaa
      721 gggatacaag gtcgcgatcg agaccctcaa cgaaggacgc atcggcatcg gcgcccagat
      781 gatcgggctg gcgaagggcg ccctcgagca ctcggtccgc tacacgaagg agcggaagca
      841 gttcggcaag ccgatcgccg aattccaggc ggtgcagcac cagctcgcgc gcgcggcggt
      901 ggaactggag gcggcgaagc tgacggtgta caacgccgcg cgcctgcgcg acgcggggcg
      961 gcccttcctg accgaggcgg ccatctgcaa gatcctgtcg tcggaagtcg ccgagcgcgt
     1021 gtcgtcgctc gcggtgaacc tgttcggcgg caacggattc gtgaaggaat acccggttga
     1081 aaagctcttc cgggacgcga agatcggcca gatctacgaa gggacgtcga acctgcagct
     1141 gcagactatc gcgaagcagc ttctcggctg accgctaccg ctccgactgc cgcggttcct
     1201 cgtcgtcgaa gagaatgcgc tgcgccgtgc gcacgggccg gggcgcgtcg ctcgcttcga
     1261 cgggctgcgg cggtgcgccg aaccgctcgc gaatggtccc cgagcggtac acgcccgtct
     1321 ccacgggcag gaggcgctcc tgcggatacg agccgctcac ccaccactcg ggttgcgcga
     1381 agtgcgggtg cgtgtcgtag gcggccgcct cgtcgcccgg ggccttgtcg gggcagcacg
     1441 cgtcccggat gctgcgctcg aagaggcgca tgtcctcgtc ggaaaactcg cggaagcgat
     1501 cctcgtcgaa cggcgggtag cgtgacagcg cgtcgcgggt gacctcgacc cgaagggtct
     1561 tccgctcgga accaatcgtg gcgtggccga cggggatcag gacgcggcgc gaccggaacc
     1621 agccgccgga gtccacgacc acgtatctga cgcggcatgc ctcggcgtcc acgagaaacc
     1681 cgtcgaggtc gccgatgcgg gtcccgtcgg gcccgcatac gtccagaccg tcgtaatcca
     1741 cgacagtcgc atctacatcc tcggcgtcga gatagcgcag gcgtgaggcg tatgtcgtgt
     1801 gctccaccgg gtccctcctc gggtgtggga ccgcaggtag caagcgaagg gccaagggcc
     1861 aagggtcaag ggtcaagggt caagggtcaa gggtcagggt ttgagcagat gctcggcgat
     1921 gaccatgcgc tggacttcgg aggtgccctg gtagatctcg gtggcgcgga cgtcgcggaa
     1981 gagccgttca gccaccgatc cgcggcggta gcccttcgag gcgagaatct gcatcgcctt
     2041 gtcggccgcg cgatgtgccg cttccgacgc gtacaacttg gccatcgcgg cttcgagcgt
     2101 cacgtccccc ggcacgagcg aggcggcctt cagcgtgagc attcgcgccg cgtcgagctc
     2161 ggtcgccatg tcggcgagca tccactggat tgcctggtaa ttgccgatcg gctgcccgaa
     2221 cgcttccctc gtcttcgcgt actcgagggc ctcttcgagc gcggcttgtc ccacgccgag
     2281 ggcctgggcg gcgatcgcca cgcgcccgcc gtccagcgcc cacatggcga tcttgaaacc
     2341 atcgccgggt gcgccgagca gcgcgtcggc atccacgcgg acgttgacga gctcgaggtc
     2401 catgcacccg aggccgcgca cgccgaggga gtctgcggtc atcacgcgcc tgatgcccgg
     2461 cgtgtccatc ggcaccagga aagcggtgat gcggcggcca agggcctgcc ccgagcgagc
     2521 gaagcgagtc gaagggtggg tggcggcgaa cacgatggcg agctcggcgg cctcggcgtt
     2581 ggcgacccag accttcttgc cattgaggac gtacccgcgc tcgtcgaggc gggcgaccgt
     2641 gtgctggttg gcggcgtccg agccggcgtg ctcttcggag agcgcgaagg cgccgagcga
     2701 ttcccccgtt gcgagccggc gcagccaggc ctgcttctgc gcgtcggtgc cgaaacgctc
     2761 gatgggctcg gtaacgagcg agttgttgac ggcggcaatc acggccacga ccgcgctcgc
     2821 ctt
//
LOCUS       DASTZR010000061         4169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_55225, whole genome shotgun sequence.
ACCESSION   DASTZR010000061 DASTZR010000000
VERSION     DASTZR010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55225"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VFV20_01355"
     CDS             <1..496
                     /locus_tag="VFV20_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287026.1"
                     /translation="AFNIVDASGSPASLLPGFLRDGRLVTAQCDQVAHTAELSVGAPG
                     ARPTRIAVVPITTRSPFFTLASGNDEILVLASAPDAPQDAYVFDPAGRLLRRIPVPAF
                     ARPGTFEFAADLSRDGRSLGFTVSEPRRDPYPDYALRAGVVDLVTGRVTYLCDRDCTW
                     LLVR"
     gene            complement(500..1555)
                     /locus_tag="VFV20_01360"
                     /pseudo
     CDS             complement(500..1555)
                     /locus_tag="VFV20_01360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(1568..1972)
                     /locus_tag="VFV20_01365"
     CDS             complement(1568..1972)
                     /locus_tag="VFV20_01365"
                     /inference="COORDINATES: protein motif:HMM:NF045191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287027.1"
                     /translation="MATTLKTETQVPTDLREWLRKVEEVGELQVVTGATAEDDIGMAT
                     ELLGRTRPSKATVFDEIPGYQKGWRVLSNGLGSFKRIAITLGLPIDASPHEMVRLWQE
                     RVRKGIPSIKAEAVKDGPVFENVMRGSQVDLL"
     gene            2084..2872
                     /locus_tag="VFV20_01370"
     CDS             2084..2872
                     /locus_tag="VFV20_01370"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5287028.1"
                     /translation="MKLEARDVVIHHRRQRYGDMLLAVDHVSLEIGEGEFVAIVGPSG
                     CGKTTFLNAIDGLIPLTSGSLSLNGRRIDRPGRDRAMVFQQPSLLPWRTVTRNVAYGL
                     EIQHLPKHEARARASRFVELVGLKGFEESYPLELSGGMQQRVNLARALAVDPEILLLD
                     EPFAALDAQTRETMQQELLRVWNATRKTAVFITHDIAEAVYLADRVFVLTARPGRLKE
                     TIRVDLARPRDLRIKHDPRFVEIETHIWESIREEAARDTMAATA"
     gene            2872..3750
                     /locus_tag="VFV20_01375"
     CDS             2872..3750
                     /locus_tag="VFV20_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5287029.1"
                     /translation="MADARRIDPVAPEQQYDSVPLPVSHDDPRVITTPLPDEGWLRRN
                     ESLVIGTVAVIVFMVAWQLVANARIWSALFLPGPADIVTAFAELIQGGELAIDIAISS
                     QEFATGYAMAAIIAIPLGLLLGWYPRARYALDPFVTFLYATPRIVLLPLFIIWFGIGI
                     ESKIAVIFLGAFFAILINTTAGVRNLDTHLIRVARSLGGNDLQIFRTIALPGSVPFIL
                     TGLRLGLGHALIGVVVGEYVAAQHGIGKMMMIAGQTFQSAQVFAGLTIIATAGLLMTL
                     LLQRLERHFDAWRPQR"
     gene            3755..>4169
                     /locus_tag="VFV20_01380"
     CDS             3755..>4169
                     /locus_tag="VFV20_01380"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEU5287030.1"
                     /translation="MTSGTADRYDVARRVLSRVTHAVAIVGAAHADERSCGTGTTMYV
                     SLSPAMIAIAEHTGSRTTRLIQASGEFSVSLLHDSQQDLAAAAGKSADGPDKFATLRI
                     RTVEAPAGMRAPGVEGAVAVLWCRVANTVETGDHLL"
BASE COUNT          597 a         1484 c         1400 g          688 t
ORIGIN      
        1 ggcattcaac atcgtcgacg cgagcggcag tcccgcatcg ctgttgcccg gcttcctgcg
       61 cgatgggcga ctcgtcacgg cgcagtgcga ccaggtcgct catacggccg agctctccgt
      121 cggcgcgccc ggtgctcggc cgacacgcat cgccgtcgta ccgatcacca cgcggtctcc
      181 gttcttcacc ctggccagcg ggaacgacga gatcctcgtg ctcgcgtcgg cgcccgacgc
      241 gccgcaggac gcctacgtgt tcgacccggc gggccggctg ctgcgcagga tcccggtgcc
      301 cgcgttcgcg cggccaggga ccttcgagtt cgcggccgac ctctcacgcg atggacggtc
      361 gctcggcttc accgtcagcg agccgcggcg cgacccgtac ccggactacg cgctgcgcgc
      421 gggcgtggtg gatctcgtga ccggcagggt cacctatctc tgcgaccgcg actgcacctg
      481 gctgctcgtg cgctgagttc tagccgcgcg gcttgtcgcc ctttccgacg agccagcccc
      541 acttcgcgag cgccttcgcc ttcgtctcgg gcgtcggcag ctgcgagcgc gggaacttgt
      601 cgcgccactc ccagggccgc gtcgcgtcga tgaccgcgcg cgacgagagg cccttgcggt
      661 cggggtggat gcgcgggtcg agcggaccgg accacatgcg cgggatgatg tcgatcgact
      721 cggacggatc cgagcgcgtg cacatcgccc agaggacctc ttcgaggtcc atcacgtcga
      781 tgtcctcgtc gacgacgacg gtgtagcggc cgaggtacgc gccggtgtgg accgtcgctg
      841 ccgcgtggcc gacctggcgc gcgtggccgg cgtagcgctg ctggatcgag atcgcgttga
      901 agagccgcga gccgccgacc tcgtggcacc acacgccgac gatgccgggg atgctcgcgc
      961 cctcgaggtc gccgcgcagc cgcgccgacc gcatgaacgc gcggtagaag gactgctcgt
     1021 ccggcgggac gttcggcggc gacgcgagga tgatcgggtc gttgcggtga tagatcgcct
     1081 caacgcgcat caccggctcc tcgcgctcac ccgaggcgta gtagccggtc cattcgccga
     1141 acggtccctc gaccatcttg tcggtcccgt cggcgaagcc ctcgatcacg atctcggcgt
     1201 ccgccgggat cggcaggccg gtgaccggac ccttcacgac gcgatagggc gtgccgcgca
     1261 cgccgccggc ccagtcgtat tcggtgatgc cgtacgggat ctcggtgcag gccgagagga
     1321 acagcagcgg gtcgccgccc ggcacgaacg cgacgggcat cttctcgccc ttcgcgaacc
     1381 acttgtcccg gtgctggcgg ccgtgcttgc cgggcgagat gtagaagccg agcgtgttct
     1441 tgttctggac catgacgcgg tacgtcccga ggttcaccca gccctcgtcc ggatccatcg
     1501 tgatgtcgaa gctgccggtg ccgaggtagc ggccgccgtc gaactcgtgc cacttcggcg
     1561 tcgggaacta cagcaggtcg acctgcgatc cgcgcatgac gttctcgaag accggtccgt
     1621 ccttgacggc ctccgccttg atgctgggga tgcccttccg cacgcgctcc tgccacagac
     1681 ggaccatctc atgtggcgac gcgtcgatgg ggaggccgag ggtgatcgcg atgcgcttga
     1741 aggagccgag gccgttggag aggacgcgcc agcccttctg gtatcccggg atctcgtcga
     1801 agacggtcgc cttcgacggg cgggtgcggc caagaagctc ggtggccatc ccgatgtcgt
     1861 cctcggccgt ggcgccggtg acgacctgga gctcgccgac ctcttcgacc ttgcggagcc
     1921 attcgcgcag gtcggtcggc acctgcgtct cggtcttcag tgtcgtggcc atcgtcgctc
     1981 tccctcgttt tgtgcgctcg gcgttcgaat gtaggcctgg cctctggtat ctgggccgaa
     2041 tgttaatacc attggaccac tgggacgcgg ggagacttca cctatgaagc tcgaagcacg
     2101 ggacgtcgtc atccatcacc gccgccagcg gtacggcgac atgctgctcg cggtcgacca
     2161 cgtctcgctc gagatcggcg agggcgagtt cgtcgccatc gtcggaccgt cgggatgcgg
     2221 gaagacgacc ttcctcaacg cgatcgacgg cctgatcccg ctcacgtcgg gctcgctctc
     2281 gctgaacggg cgccgcatcg accgtccggg acgcgaccgg gcgatggtgt tccagcagcc
     2341 gagcctgctt ccctggcgca ccgtcacacg gaacgtcgcc tatggcctgg agatccagca
     2401 tctcccgaag cacgaggcgc gcgcacgcgc gtctcgcttc gtcgagctcg tgggtctcaa
     2461 aggcttcgag gagtcctacc cgctcgagct ctccggcggc atgcagcaac gcgtgaacct
     2521 cgcgcgcgct ctcgcggtcg atcccgagat cctgctcctc gacgagccgt tcgcagcgct
     2581 cgacgcgcag acgcgcgaga cgatgcagca ggagctcctc cgcgtctgga acgcgacgcg
     2641 caagacggcc gtcttcatca cccacgacat cgccgaggcg gtctacctcg ccgatcgcgt
     2701 gttcgtcctc accgcgcggc cgggacggct caaggagacg atccgcgtcg acctcgcgcg
     2761 accgcgcgac ctccgcatca agcacgatcc gcggttcgtc gagatcgaga cccacatctg
     2821 ggagagcatc cgcgaggagg cggcccgcga cacgatggcc gcgaccgcct gatggccgac
     2881 gcacggcgca tcgatcccgt ggcgcccgag cagcagtacg actcggtgcc gctcccggtg
     2941 tcccacgacg atccgcgcgt gatcacgacc cccctgcccg acgaggggtg gctgcggcgg
     3001 aacgagtcgc tcgtgatcgg caccgtcgcg gtgatcgtct tcatggtcgc gtggcagctc
     3061 gtggcgaacg cgcggatctg gtccgcactg ttcctgccgg gtccggccga catcgtcacg
     3121 gccttcgccg agctcatcca gggcggggag ctggccatcg acatcgccat ctcgtcgcag
     3181 gagttcgcga ccggctacgc catggccgcg atcatcgcga tcccgctcgg cctgctgctg
     3241 ggctggtatc cgcgcgcgcg ctacgcgctc gatccattcg tcacgttcct ctacgcgacg
     3301 ccgcgcatcg tgctcctgcc gctcttcatc atctggttcg ggatcggcat cgagtcgaag
     3361 atcgcggtga tcttcctcgg cgcgttcttc gcgatcctca tcaacaccac ggcgggcgtg
     3421 cgcaacctgg acacgcatct catccgggtc gccaggtcgc tcggcggcaa cgacctgcag
     3481 atcttccgca ccatcgcgct gccgggctcg gtgccgttca tcctcacggg actgcgcctg
     3541 ggcctcggcc acgccctcat cggcgtggtg gtgggggagt acgtcgccgc ccagcacggg
     3601 atcggcaaga tgatgatgat cgcgggacag accttccaga gcgcgcaggt gttcgccggc
     3661 ctcacgatca tcgcgaccgc cggactgctc atgacgctgc tgctccagcg gctcgagcgc
     3721 cacttcgacg cgtggcgccc gcagcggtag cagggtgacc agcgggaccg ccgaccgcta
     3781 cgacgtcgcg cgccgcgtgc tgtcgcgggt cacgcacgcg gtggcgatcg tgggcgcggc
     3841 ccacgccgac gagcgctcct gcggcaccgg cacgacgatg tacgtctccc tctcgccggc
     3901 gatgatcgcg atcgcggagc acaccggatc gcgcaccacg cgtctgatcc aggcgagcgg
     3961 cgagttcagc gtctcgctcc tgcacgactc ccagcaggac ctcgccgccg cggcggggaa
     4021 gagcgcggac ggccccgaca agttcgcgac gctccgcatc cggacggtgg aggcgccggc
     4081 gggaatgcgc gcacccggcg tggagggcgc cgttgccgtc ctctggtgcc gcgtggcgaa
     4141 caccgtcgag accggcgacc acctgctct
//
LOCUS       DASTZR010000062         5441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_81820, whole genome shotgun sequence.
ACCESSION   DASTZR010000062 DASTZR010000000
VERSION     DASTZR010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81820"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1128
                     /locus_tag="VFV20_01385"
     CDS             91..1128
                     /locus_tag="VFV20_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287031.1"
                     /translation="MTVGHRCRLAVILALTLSLLGACSGGGGGSAPAASGSGGCGTQA
                     TFILANPGGLTCLDDTARSIGKVVKLPDQSAPSTPTLHPSGSAIAFALTGQPDPKRGF
                     GSDIYVVNLDGSELRPVVQHEDNNVFYASPRFDSSGNVLYAPRRAAVLANGSFVGNED
                     AIVRIDLRTGQSTRIIKDGADPAISADGKTLVYVHLKDSQIDALWRADIDGSNAAPLL
                     KTKDTFWYLQAPRFSPSSCEVVFSAAGHTAGGVPGGAAAAMRPWVPGGTPLAHLNIPS
                     ELFLAPCDGSGVKAVGQTGDDVVPSWSPDGTRIAYVGTGGFFVLDTRSGTTRTIANGQ
                     DFFFGDLVWLK"
     gene            1205..1786
                     /locus_tag="VFV20_01390"
     CDS             1205..1786
                     /locus_tag="VFV20_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287032.1"
                     /translation="MLRVALSCLALLVTVSCGSSPASTAGTSQPATAAAATPTVAPTA
                     AATPRPPTTAPIPAFEDATFTGTWTNQTFGSTGGATMAVKVDRAALTLGVTLTLTGNV
                     FGTAAPAPETFTLPINAAGATYTGKSKTFGDVTATLKADGTFTFRGENVPSNRVKGID
                     GSGTWTTSGINFTYNVALSDGSAAKGVVSLKKS"
     gene            complement(1800..2903)
                     /locus_tag="VFV20_01395"
     CDS             complement(1800..2903)
                     /locus_tag="VFV20_01395"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019609751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEU5287033.1"
                     /translation="MIPLETLRAAPKVLLHDHLDGGLRPATVVELAREQGYTKLPTVD
                     PAELGRWFHASAASGSLALYLRGFAHTIAVMQTPEAIERVAFECGEDLARDGVVYAES
                     RYAPIFSTARGLTLREVVESVSRGFARAEARYPIALRQIVCAMRDRTDSLEMARLAVS
                     YRDRGVVGFDIAGEEAGHPPKEHLEAFDLCHRENFSITIHAGEGFGPQSIWQAIQVCG
                     AHRIGHGVRLIEDMRIEGGAVVSLGPLAQYIRDKRIPLELCPSSNVDTGAAASLESHP
                     IRHFLAQRFRVTVSTDNRLMSNVSLSDELHRLAQCLGLRLDDVEKLTINAMKSAFVGY
                     DERLALIYGTIKPGFLKLRGEPALAGYRLPPSA"
     gene            2991..5123
                     /locus_tag="VFV20_01400"
     CDS             2991..5123
                     /locus_tag="VFV20_01400"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEU5287034.1"
                     /translation="MDGIWETLRERLDYAQFVPHPLADTERADLHHRDGRPYTMLKNP
                     HGDRGAGTYLRLEADDVALFELMDGTRTVQEILLAHLERRGVLAVDRLARLTAALAAN
                     GFFGEERPRVYERLARQRAMRDPLVRASLWLRRLIAWDIARWRNADIAVERVYRWGGR
                     LAFTPVGAAVVGLICLGGIVAWTSEVRAGRHALVTVDGSFVLGIVALIALQVLSISVH
                     EAGHALAIRHHGRRVRQLGLAVYYVFPCVYVDATDMSMSSRRARIVVALAGPIGGLAV
                     GALCAFVAATDAGVLGGLAFKAASLFIFQFALNLMPILELDGYHVLSDVLDAPMLRQR
                     AIAFARGSIVRKLRRRERWSRGQVGLAVYGVFAIVTSLLMILFSLALWESRVAIAARE
                     LFGLGPVGFAILAALVVVFIGPLVLALALRVVGWARAAARAAAARTRRAQHALLVERA
                     RALARVPFLVGLNGPSLLAIASHMRDVHVPGGSNVVTIGEPGDRFYVVRSGRLQALDA
                     DGAVLTTMTAGDGFGELALLDRTPRQATVRALEDAELWALDRGHFERWVSARYEIAAR
                     IRASRAERAALAALPFFGGLEPQELDRILPRLQSVRVPAGTAVVREGEPGDRYYVIRS
                     GEAEVSQRGAALRRIGPGAGFGELALLLGRPRSATVTAVTELALASLGRAEFAWLVRK
                     SGETMGELRARTAHYVGDVGLGTAVRGA"
     gene            5221..5343
                     /locus_tag="VFV20_01405"
     CDS             5221..5343
                     /locus_tag="VFV20_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287035.1"
                     /translation="MLDVFRGTRHETLRALVVEDALHCGICKPRGEPVELPELE"
BASE COUNT          763 a         1912 c         1943 g          823 t
ORIGIN      
        1 cgtgagcgcc gtgcagcact tcgcgcgcgc atcggcgcgc atccatggca gcctgcgcga
       61 ccaggcgaag cgcgcgggcg aagtgatcgc gtgactgtgg gtcatcggtg ccgcctcgcc
      121 gtcatcctcg ccctgacgct ctcgctgttg ggggcttgta gcggtggcgg cggcggatcg
      181 gcgcccgcgg cttcggggag cggcggatgc ggcacgcagg cgacgttcat cctggccaat
      241 ccgggcgggc tcacctgcct tgacgacacg gcgcgttcga tcggcaaggt cgtgaagctc
      301 ccggatcaga gcgcgccctc cacgccgacc ctgcacccgt cgggatcggc gatcgcgttc
      361 gcgctcaccg gccagccgga ccccaaacgc ggatttgggt ccgacatcta cgtcgtgaac
      421 ctcgacggca gcgagctccg tccggtcgtg cagcacgagg acaacaacgt cttctacgcg
      481 agcccgcgct tcgattcgag cggcaacgtc ctctacgccc ctcggcgcgc ggcggtcctc
      541 gcgaacggct ccttcgtcgg caacgaggac gcgatcgtcc gtatcgatct gcgcaccggg
      601 cagtcgacgc ggatcatcaa ggacggcgcc gaccccgcca tcagcgctga cggcaagacg
      661 ctcgtctacg tccacctcaa agactcgcag atcgacgcgc tgtggcgcgc ggacatcgat
      721 ggctcgaacg ccgctcccct gctgaagacg aaggacacgt tctggtatct ccaggcgccg
      781 cggttctcgc cgtcgagctg cgaggtcgtg ttctcggcgg ccggccacac cgccggcggc
      841 gttccgggcg gcgcggccgc ggcgatgcga ccctgggtgc ccggcggcac gccgctcgcg
      901 cacctcaaca tcccatcgga gctgttcctc gccccgtgcg acggatcggg cgtgaaggcc
      961 gtcgggcaga cgggcgacga cgtcgtgccg tcctggtcgc cggacgggac gcgcatcgcg
     1021 tacgtcggca ccggcggctt cttcgtgctg gacaccagat ccggcacgac ccgcacgatc
     1081 gcgaacggcc aggacttctt cttcggcgac ctggtctggc tcaagtagcg gcccgccgct
     1141 gacgcgccga gcgctcggcc gtatgctcgc cgctgtccag aggttcgggt ggaggtggaa
     1201 acccatgctg cgcgtcgcgc tgtcgtgtct tgcgctcctc gtcaccgtgt cctgcggatc
     1261 gtcgccagcg tcgacggccg gcacgagcca gccggcaacg gcggcggcgg cgacccccac
     1321 cgtcgccccg acggcggcgg cgaccccgcg accgccgacg acggcgccga tccccgcgtt
     1381 cgaggacgcg acgttcaccg gcacgtggac gaaccagacg ttcggatcga cgggcggcgc
     1441 caccatggcc gtgaaggtcg atcgcgcggc gctcacgctc ggtgtcacgc tcacgctcac
     1501 ggggaacgtc ttcggcaccg cggcaccggc accggaaaca ttcacgctgc cgatcaatgc
     1561 ggccggcgcg acctacaccg ggaagtcgaa gaccttcggg gacgtcaccg cgaccttgaa
     1621 ggcggacggc accttcacct tcaggggcga gaacgtcccg tcgaaccgcg tgaagggcat
     1681 cgacgggagc gggacgtgga cgacatccgg gatcaacttc acgtacaacg tggccctgag
     1741 cgacggcagc gccgcgaagg gcgtcgtcag cctgaaaaag agctagacgg cacgggcggt
     1801 caggcgctcg gcgggagtcg atatcccgcc agcgccggct cgccgcgcag cttcaggaat
     1861 cccggcttga tcgtgccgta gatcagggcg agccgctcgt cgtagccgac gaaggccgac
     1921 ttcatcgcgt tgatcgtcag cttctccacg tcgtcgaggc gcaggccgag gcactgcgcg
     1981 aggcggtgca gctcgtcgga gaggctcaca ttcgacatga gccggttgtc ggtgctcacc
     2041 gtgacgcgaa agcgctgcgc caggaagtgt cgtatcggat gtgactcgag ggaggccgcg
     2101 gcacccgtgt cgacgttgct cgacgggcac agctccagcg ggatgcgctt gtcgcggatg
     2161 tactgcgcca gcggaccgag cgagacgacc gcgccacctt cgatgcgcat gtcctcgatg
     2221 agccgcacgc cgtggccgat gcgatgcgcg ccgcagacct ggatcgcctg ccagatcgac
     2281 tgcggtccga acccttcgcc cgcatggatg gtgatggaga agttctcgcg atggcagaga
     2341 tcgaacgcct cgaggtgctc cttcggcgga tgccccgcct cctcgccggc gatatcgaag
     2401 ccgacgacgc cgcgatcccg atacgagacc gcgagccggg ccatctcgag cgaatcggta
     2461 cggtcccgca tcgcgcagac gatctgacgg agggcgatcg gataccgcgc ctcggcgcgc
     2521 gcgaatcccc gactcaccga ctcgacgacc tcgcgcagcg tgagaccgcg cgccgtgctg
     2581 aagatcggcg cgtagcggct ctcggcgtac acgacgccgt cgcgcgcgag atcctcaccg
     2641 cactcgaacg cgacgcgctc gatcgcctcc ggcgtctgca tcacggcgat ggtgtgcgcg
     2701 aagccgcgga gatagagcgc gagcgatccg ctcgcagcgc tcgcatggaa ccagcgaccg
     2761 agctcggcgg gatcgaccgt cgggagcttc gtgtagccct gctctctcgc gagctcgacc
     2821 accgtcgcag gccgcaggcc gccgtcgagg tggtcgtgga ggagcacctt tggcgccgcc
     2881 cggagcgtct ccagcgggat catgtgcggc ggaagtatgc gctcgaaatg ccaccgcggt
     2941 ggcgcccgac gggcgatcgc cggatagcat gcctctggcg gagggcgctt atggatggga
     3001 tctgggagac gctccgcgag cgtctcgact acgcacagtt cgttccacac ccgctcgccg
     3061 acaccgaacg tgcggacctc caccatcgcg acggacgtcc ctacacgatg ctcaagaatc
     3121 cacacggcga tcgcggcgcg ggaacgtacc tgcgcctcga ggccgacgac gtggcgctgt
     3181 tcgagctcat ggacggcaca cgcaccgtcc aggagatcct gctcgcgcac ctcgagcggc
     3241 gcggcgtgct cgcggtcgac cggctcgcgc gcctcacggc ggcgctggcg gccaacggct
     3301 tcttcggcga ggagcggccg cgggtctatg agcgcctggc gcggcagcgc gcgatgcgcg
     3361 acccgctggt gcgcgcctcg ctctggctcc ggcgactgat cgcctgggac atcgcgcgct
     3421 ggcggaacgc ggacatcgcc gtcgagcggg tgtaccgctg gggcggcagg ctcgcgttca
     3481 cgccggtcgg cgccgcggtg gtcggcctca tctgcctcgg cggcatcgtc gcctggacct
     3541 ccgaggtccg cgccggacgg cacgcgctcg tcaccgtcga cggctcgttc gtcctcggca
     3601 tcgtcgcgct catcgcgctg caggtgctct cgatctcggt ccatgaggcc ggacacgcgc
     3661 tcgccatccg gcaccacggc cggcgcgtga gacagctggg gctggccgtg tactacgtct
     3721 tcccgtgcgt gtacgtggac gccacggaca tgtccatgag cagccggcgc gcgcggatcg
     3781 tcgtcgcgct cgcggggccg atcggtggac tcgcggtcgg agcgctctgc gcgttcgtgg
     3841 cggcgaccga cgcgggcgtc ctcggcggtc tggcgttcaa ggcggcgagc ctgttcatct
     3901 tccagttcgc cttgaatctc atgcccatcc tcgagctcga tggctaccac gtcctctcgg
     3961 acgtgctgga cgcgccgatg ctccgccagc gtgcgatcgc gttcgcgcgc ggctcgatcg
     4021 tgcggaagct gcgccgccgc gagcgctggt cgcgcggcca ggtcggcctc gccgtgtacg
     4081 gcgtgttcgc catcgtcacc tcgctcctga tgatcctgtt ctcgctcgcg ctgtgggaat
     4141 cgcgcgtcgc gatcgccgcg cgcgagctgt tcgggctcgg tccggtcggc ttcgcgatcc
     4201 tcgcggcgct cgtggtcgtc ttcatcggcc cgctcgtgct cgcgctcgcg ctgcgggtcg
     4261 tcggatgggc acgcgccgcg gcgcgggccg cggccgcgcg gacacgccgg gcccagcacg
     4321 cgctgctcgt cgagcgagcg cgcgcgctgg cgcgcgttcc gttcctcgtc gggctgaacg
     4381 ggccgtcgct gctcgcgatc gcatcgcaca tgcgcgacgt ccacgtgccc ggaggctcga
     4441 acgtcgtgac catcggcgag cccggggacc gcttctacgt cgtgcgctcc ggtcggctgc
     4501 aggcgctcga cgccgacggg gcggtcctca cgacgatgac ggccggcgac ggcttcggcg
     4561 agctcgcgct cctcgaccgc acgccgcgtc aggcgacggt gcgcgcgctg gaggatgccg
     4621 agctgtgggc gctcgaccgc ggccacttcg agcgctgggt gagcgcgcgc tacgagatcg
     4681 cggcacggat ccgcgcctcc cgcgccgagc gggcggcgct cgccgcgctc ccgttcttcg
     4741 gcggtctcga gccccaggag ctcgaccgca tcctgccgcg gctccagagc gtccgcgtcc
     4801 ccgcggggac cgcggtcgtc cgcgagggcg agccgggtga ccgctactac gtgatccggt
     4861 ccggggaggc cgaggtgtcc cagaggggcg ccgccctgcg ccgcatcggc cccggcgccg
     4921 gtttcggcga gcttgcgctg ctcctcggcc ggccgcgatc cgcgacggtc accgccgtta
     4981 cagagctggc cctcgcgagc ctggggcggg cggagttcgc gtggctcgtt cgaaagtccg
     5041 gggaaacgat gggcgaactt cgggcgcgga cggcgcatta tgtcggggac gtcgggctcg
     5101 gcacggcagt gaggggggcg tagcgaggtg gattgctggg tctgcgagcg cactgcgctg
     5161 ggggcctgcc gcttctgcgg gagaggtacc tgccgcgagc acgcgaggac gcatccgttc
     5221 gtgctcgacg tcttccgcgg aacgaggcac gagacgcttc gcgcgctggt cgtggaggac
     5281 gcgctccact gcggcatctg caagccacgc ggcgagccgg tggagctgcc cgagctcgag
     5341 tagcggaaag gaggggacgg caggggcggg accgcgctcg cgcggccgct attgtgtgat
     5401 caggatcaca gaggacatga cacggggatg gacgagccga a
//
LOCUS       DASTZR010000063         2110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1153152, whole genome shotgun sequence.
ACCESSION   DASTZR010000063 DASTZR010000000
VERSION     DASTZR010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153152"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VFV20_01410"
     CDS             complement(<1..752)
                     /locus_tag="VFV20_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU5287036.1"
                     /translation="MKRIALITALVAVFAAAFAAWGLAQPVGKATTSAQAAKKLPPLP
                     ANIKERKRWIIGVKCDVPPFGYIDVRGRNAGFDVEIARWFARYAFGRENRVSFVCAPT
                     PAREPLLTTDRADLVISTFTYTADRDTRIDFSNAYYKATGRLLVKNDSTITRLADIKG
                     KRISTTSGSIYDRWMKRCFPDTQVTVADSFTNAALAFNQGRADGVMFDDTSLALIAAT
                     DPSSKLTDDTFLEAPYGIGIKQGHTAMKRWVDS"
     gene            complement(749..1525)
                     /locus_tag="VFV20_01415"
     CDS             complement(749..1525)
                     /locus_tag="VFV20_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010987802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU5287037.1"
                     /translation="MVRLEGVRKSFGDLLVLDGIDLTVSPGEVLVIVGPSGSGKSTLL
                     RCVNLLEPVQAGRVFLEGQEITARGTDVSRVRQRIGMVFQQFNLFPHLKVIDNVTLAA
                     RRIRRVSRREAESKAEDLLARVGLADKARQYPHQLSGGQQQRVAIARALMMEPHVMLF
                     DEVTSALDPELVGEVLIVMRDLARDGMTMLVVTHEMQFARDVGDRVVFMDNGKIVEQG
                     PPGDVLDNPQHERTRRFLRRTLQLADSLEELEINEEGVAE"
     gene            complement(1619..>2110)
                     /locus_tag="VFV20_01420"
     CDS             complement(1619..>2110)
                     /locus_tag="VFV20_01420"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HEU5287038.1"
                     /translation="GATPRAPLSLPPGGAHWYVLGGFPTPDAETFLSSLRLGGEWLDG
                     FLAEHGLDHGRLVLGGFSQGGVMSYALGLGEGRPRPRAIVAMSSFIPRVPGWSVDLAP
                     PLPPVAVAHGTYDGVIPPEWGREARTILEDAGAEVLWRESPMDHTIDPQFLLEVRARL
                     LTA"
BASE COUNT          311 a          737 c          743 g          319 t
ORIGIN      
        1 gagtcgaccc accgcttcat ggccgtgtgg ccctgcttga tgccgatgcc gtacggcgcc
       61 tcgaggaacg tgtcgtcggt gagcttcgac gacggatcgg tcgccgcgat cagcgccagc
      121 gacgtgtcgt cgaacatcac cccgtcggcg cggccctggt tgaacgcgag cgccgcgttg
      181 gtgaagctgt ccgcgacggt cacctgcgtg tcggggaagc agcgcttcat ccaccggtcg
      241 tagatcgagc cgctcgtcgt cgagatccgc ttgcccttga tgtccgcgag ccgggtgatc
      301 gtggagtcgt tcttcacgag caggcggccg gtcgccttgt agtacgcgtt cgagaagtcg
      361 atgcgcgtgt cgcggtcggc cgtgtacgtg aaggtggaga tgacgagatc ggcgcggtcg
      421 gtcgtgagca gcggctcgcg ggccggcgtc ggcgcgcaga cgaagctcac ccggttctcg
      481 cgtccgaacg cgtagcgtgc gaaccagcgc gcgatctcga cgtcgaagcc cgcgttgcgg
      541 ccgcgcacgt cgatgtagcc gaacggcggc acgtcgcatt tcacgccgat gatccagcgc
      601 ttgcgctcct tgatgttcgc gggcagcggc ggcagcttct tcgcagcctg tgccgatgtc
      661 gtcgccttgc ctaccggctg tgcgagaccc caggccgcga acgccgcagc gaagaccgcg
      721 acgagcgcgg tgatcagtgc gattcgcttc attctgcaac tccctcctcg ttgatttcga
      781 gctcttcgag cgagtccgcg agctgcagcg tccggcgcag gaagcgccgc gtccgctcgt
      841 gctgcgggtt gtcgagcacg tcgccaggtg gtccctgctc gacgatcttt ccgttgtcca
      901 taaagacaac gcggtctccg acgtcgcgcg cgaactgcat ctcgtgcgtg acgacgagca
      961 tcgtcatgcc gtcgcgcgcg aggtcgcgca tcacgatcag cacctcgccc acgagctcgg
     1021 ggtcgagcgc cgacgtgacc tcgtcgaaga gcatgacgtg cggctccatc atcagcgcgc
     1081 gcgcgatcgc gacgcgctgc tgctggccgc cggagagctg gtgcgggtac tggcgcgcct
     1141 tgtcggcgag gccgacgcgc gcgagcaggt cctccgcctt cgactccgcc tcgcgccgcg
     1201 acaccctgcg gatgcggcgc gcggcgagcg tgacgttgtc gatcaccttc aggtgcggga
     1261 agaggttgaa ctgctggaag accatcccga tccgctgccg cacgcgcgag acgtcggtgc
     1321 cgcgcgcggt gatctcctgc ccctcgagga agacgcggcc ggcctgcacc ggctcgagca
     1381 ggttcacgca gcgcagcagc gtcgacttcc cggagcccga gggcccgacg atgacgagca
     1441 cctcgcccgg gctgaccgtc aggtcgatgc cgtcgaggac gagcaggtcg ccgaagctct
     1501 tgcgcacgcc ctccagacgc accatcggcg tagccgacgt cgacggcatg acgtcctcct
     1561 gccgcaaagc tccctgatcg ccggccaaac cctgaccagg gttgtacccc gcccgcggct
     1621 aggccgtcaa caggcgcgcc cgaacctcga gcaggaactg cgggtcgatc gtgtggtcca
     1681 tcggcgactc gcgccagagg acctcggcgc cggcgtcctc gaggatcgtg cgcgcctcgc
     1741 ggccccactc gggcgggatc accccgtcgt aggtgccgtg cgcgacggcg acgggaggca
     1801 gcggcggcgc caggtcgacg ctccagcccg ggacgcgcgg gatgaacgag gacatggcga
     1861 cgatggcccg cgggcgcggc cggccctcgc cgaggccgag cgcgtagctc atgacgccgc
     1921 cctgcgagaa cccgcccagc accagccggc cgtggtcgag gccgtgctcg gcgaggaagc
     1981 cgtcgagcca ctcgccgccg aggcgcagcg aggacaggaa ggtctccgcg tccggcgtcg
     2041 ggaagccgcc gagcacgtac cagtgcgcgc cgcccggcgg cagcgagagc ggcgcgcgcg
     2101 gcgtcgcgcc
//
LOCUS       DASTZR010000064         3730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1257233, whole genome shotgun sequence.
ACCESSION   DASTZR010000064 DASTZR010000000
VERSION     DASTZR010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257233"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VFV20_01425"
     CDS             complement(<1..269)
                     /locus_tag="VFV20_01425"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU5287039.1"
                     /translation="MRPIQAVLALLMLISVACGSAEAASPSAVGQRIAVVMSDYAFSP
                     AVIEVPADEQFTLDIRNAGRIEHDLTVEALRFKVVVRSGRSATRVL"
     gene            655..2262
                     /locus_tag="VFV20_01430"
     CDS             655..2262
                     /locus_tag="VFV20_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017292209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEU5287040.1"
                     /translation="MRHGLGFLVDALGLDRVIPFHRGLLGHPKRAEPYTRPEHVRMAL
                     EDLGPTFVKLGQIASTHGDLLRPDYQHELARLQDGVAPAPTDSIRAAIEAELGASIET
                     AFASFGDEPVAAASIGQAHAARLHDGTEAIVKVRRPGVIANVRSDLTLIRSLANAAST
                     RSALAERYDVVGIVDEFAATLLAELDYEREAESIARFAANFAGDPTVRIPSVFRQVSG
                     DGVLTLERLRGIRIDDLAALDAAGIDRAALAARTSRMLMKMVFEDGFFHADPHPGNLF
                     VESDGRIGLIDFGMTGTIDETTRDRLVDVLLALTGRDPSRLADAFLALGFARGRVDRT
                     LLERDLARLIAPYYERALGDISIGALLQETLEVIRRHRLVLPRELALLVKTLIMSDGI
                     ATHLAPDFKLMTVLIPYAKRMLARRLSPEVLGPRLAGAATEAARLALTLPRRVDQLLG
                     ALERGDLEIPIRPAGFEPIAERFERSADRVVLGIVAAAFMNGLAVFVSVYHPAGWQAQ
                     APLALIFGLAAGIGAYLVRSVMRPARR"
     gene            complement(2290..2559)
                     /locus_tag="VFV20_01435"
     CDS             complement(2290..2559)
                     /locus_tag="VFV20_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEU5287041.1"
                     /translation="MITAVVLITAERGAIPKLGEQLAAVPGVSEVYSVTGDFDFIAMV
                     RAREHEELADIVTRRIAQVAGVARTQTHVAFKAYSKHDLETLFAP"
     gene            complement(2560..2763)
                     /locus_tag="VFV20_01440"
     CDS             complement(2560..2763)
                     /locus_tag="VFV20_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287042.1"
                     /translation="MISSAARDRIRLAIDQLEEGFHRYEEQSTADINDSYGAALARGK
                     AMGMQEAAHLVKAALHDVDVKSL"
     gene            complement(2821..3015)
                     /locus_tag="VFV20_01445"
     CDS             complement(2821..3015)
                     /locus_tag="VFV20_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287043.1"
                     /translation="MEERKVVKERTVVEREDPVAEDVTNVNIGPDGSAQVQQEGEVID
                     EPVTSTTVRQETTVEERRAP"
     gene            complement(3085..>3730)
                     /locus_tag="VFV20_01450"
     CDS             complement(3085..>3730)
                     /locus_tag="VFV20_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287044.1"
                     /translation="AGVAENGVMLIRPAHLRPGARPPRWRGGVGGLRRMTMKGVHSMA
                     IGAVALAAVFATGCGQTGGAASGGAVTAAAPDPANATFTIEKDTITLTNGRSERPSAP
                     GSSSSAMTTLDQRTSGDVDGDGRADSVVILVNQPGGSGTFYYVGVLLNASGGTSATPA
                     LLLGDRVKVNDVRIDGKTIVVELLDRAPGQPMIASPSVSVTKRFSVDGGALKGE"
BASE COUNT          542 a         1345 c         1237 g          606 t
ORIGIN      
        1 aggactcgcg tcgccgaccg gccagatctc acgaccacct tgaaacggag cgcctcgacg
       61 gtcaggtcgt gctcgatccg cccggcgttg cggatgtcca gcgtgaactg ctcgtcggcg
      121 ggcacctcga tgaccgccgg cgagaacgcg tagtcggaca tcacgacggc gatccgctgc
      181 ccaacggccg acgggctcgc ggcttcggcc gagccgcacg ccacggagat gagcatgaga
      241 agagcgagca ctgcctggat cggtctcact tcgaactctc ctgtccgcgg cgtcacgccg
      301 cgctacttca tttgtccgtc gcaaccgcgc cggtcgtggg catcgtcgga tgagcgggtc
      361 ccgaccgaca gacggcggac acgagatccg cgcgggtgct accccgagtt caggggggtg
      421 aggctcgccg ggacgccagc gccgacagct gggaattccg gctagcaccc atccccatct
      481 cctgccgccc gtctacgcac gccggagcgc cgcgccgagg attccgtcgt cgcggaccgc
      541 gcaggaaagg agatcgggga catgaaggca gctggtgccg ctcacacgcg tacggacgag
      601 ccatcggtcg cgcgcggtca ggtggcacgt tcgcgcgaga tcgtgcacgt attgatgcgt
      661 cacggcctcg ggttcctcgt cgatgcgctc ggcctggacc gcgtcatccc cttccaccgc
      721 ggtctccttg gccaccccaa acgcgcggag ccatatacac ggcccgagca cgtccgcatg
      781 gcgctcgagg acctcggccc cacgttcgtc aagctcggcc agatcgcatc gacgcacggc
      841 gacctgctcc gaccggacta ccaacacgag ctcgcgcgtc tccaggacgg ggtggcccca
      901 gcacccacgg actcgatccg cgcggccatc gaagccgagc tcggggcgtc gatcgagacg
      961 gcgttcgcgt cgttcggcga cgagcccgtc gccgcggcct cgatcggaca agcgcatgcc
     1021 gctcgtctgc acgacggtac cgaagccatc gtcaaagtgc ggcggccggg cgtgatcgcg
     1081 aacgtccgat ccgacctcac gctcatccgc agcctcgcga acgcggctag cacacgctcc
     1141 gcgctggccg aacgctacga cgtcgtcggc atcgttgacg agttcgccgc gacgctcctc
     1201 gcggagctgg actatgagcg cgaggccgag agcatcgctc ggttcgccgc gaacttcgcc
     1261 ggcgatccga cggtgcgcat ccccagcgtg ttccggcagg tcagcggcga tggggtgctc
     1321 accctcgagc gccttcgcgg catcaggatc gacgacctcg cggcgctgga cgcggcgggc
     1381 atcgatcgcg cggcgctcgc ggcgcgcacg tcgaggatgc tcatgaagat ggtgttcgag
     1441 gacggcttct tccacgccga tccgcacccg ggcaatctgt tcgtcgagtc cgacgggcgc
     1501 atcggcctga tcgatttcgg catgacgggc acgatcgatg agacgacacg ggaccggctg
     1561 gtcgacgtgc tcctggcgct caccggcagg gatccctcgc gcctcgccga cgcattcctc
     1621 gcgctgggtt tcgcccgcgg tcgcgtcgat cggacactgc tcgagcggga cctcgcgcgg
     1681 ctcatcgcgc cgtactacga gcgcgcgctc ggcgacatct cgatcggtgc gctgctccag
     1741 gagacgctcg aggtgatcag gcggcatcgc ctcgtgctgc cacgggagct ggcgctcctg
     1801 gtgaagacac tgatcatgag cgacggtatc gcgacgcacc tcgcgcccga cttcaagctc
     1861 atgaccgtgc tgatcccgta cgcgaaacgc atgctggcgc ggcgactctc gccggaggtg
     1921 ctcggccccc gcctcgccgg cgccgcgacg gaagccgcac gtctcgcgct cacgctaccg
     1981 cgccgcgtcg accagctcct tggggcgctc gaacggggcg acctcgagat ccccattcga
     2041 cccgcgggct tcgagccgat cgccgagcgc ttcgagcgat ccgcggatcg cgtcgtgctc
     2101 gggatcgtgg ccgccgcgtt catgaacggg ctcgcggtct tcgtttcggt gtaccaccca
     2161 gcgggctggc aggcgcaggc gccgctcgcg ctcatcttcg ggctcgcggc cggcattggt
     2221 gcgtatctcg ttcgatccgt catgcgaccg gcaaggcgat agatcaggga aggcgcccgc
     2281 ggcgccgccc tacggggcga acagcgtttc gaggtcgtgc ttgctgtagg ccttgaatgc
     2341 cacgtgcgtc tgcgtgcgag cgacccccgc cacctgtgcg atgcgccgcg tcacgatgtc
     2401 ggcgagctcc tcgtgttccc gggcgcgcac catcgcgatg aaatcgaagt cgccggtgac
     2461 cgagtagacc tcggacacgc cgggcaccgc ggcgagctgc tcgccgagct tcgggatcgc
     2521 gccccgctcc gccgtgatga ggacgaccgc agtgatcacc taaaggctct tcacgtccac
     2581 gtcgtggagc gcggccttca ccagatgggc cgcctcctgc atgcccatcg ccttgccgcg
     2641 agcgagcgcc gctccgtagg agtcgttgat gtcggcggtg gactgctcct cgtagcgatg
     2701 gaacccctcc tcgagctggt cgatggcgag cctgatgcgg tcgcgcgcgg cactgctgat
     2761 cacggaagcc tcccggcggt cgattcgctc cgaggatacc gccggaaggc tccgcgtcgt
     2821 ctagggtgcg cgccgctcct cgaccgtggt ctcctggcgg accgtggtcg atgtcacggg
     2881 ttcgtcgatg acctcgccct cttgctggac ctgcgccgag ccgtcgggcc cgatgttcac
     2941 gttcgtgacg tcctcagcga cgggatcctc gcgctcgacc acggtgcgct ccttgacgac
     3001 ctttcgctct tccatccaga tctccttctc cgcagtgtgc ggaggttgag gagcaggctc
     3061 gatgccatcg tccgatctgc gccgctactc ccccttgagc gcgccgccat cgacgctgaa
     3121 gcgcttcgtg accgagacgc tgggcgaagc gatcatgggc tgccccggcg cgcggtcgag
     3181 gagctcgacg acgatcgtct tcccatcgat gcggacgtcg ttcaccttga cccggtcgcc
     3241 gaggaggagc gccggcgtgg cgctggtgcc gccgctcgcg ttcagcagca cgccgacgta
     3301 atagaaggtg ccgctgccgc cgggctggtt cacgaggatg accacgctgt cggcgcggcc
     3361 gtcgccgtcc acgtcgccgc tggtcctctg gtcgagggtc gtcatcgcgc tgctcgacga
     3421 acccggcgcc gacggacgct ccgagcggcc attcgtgagc gtgatcgtgt ccttctcgat
     3481 cgtgaacgtc gcgttcgcgg ggtcaggagc cgcggcggtc accgccccgc ctgacgccgc
     3541 tccgcccgtc tggccgcagc ccgtcgcgaa caccgcggcg agcgcgaccg caccgatcgc
     3601 catgctgtga acgcccttca tcgtcatcct ccgaagaccg ccgaccccgc cacgccacct
     3661 cggcggccgc gcgcccggcc gcagatgcgc gggacggatc agcatgacgc cattctccgc
     3721 cacgcctgcg
//
LOCUS       DASTZR010000065         2825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_291493, whole genome shotgun sequence.
ACCESSION   DASTZR010000065 DASTZR010000000
VERSION     DASTZR010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291493"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..2049)
                     /locus_tag="VFV20_01455"
     CDS             complement(127..2049)
                     /locus_tag="VFV20_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEU5287045.1"
                     /translation="MGTELDFGAFAVLDEVTRARLLADATRVSFAPGSDVIREGDPGD
                     AVYAIVGGRVRVTQRGTVVASPRAPLVVGEMAVLSDEARSATVTALTRVRAYRMSAAA
                     IRDLAVSRPDFRRELAGFARVRVGNNFLRRSSPFADLPASAIEALAAKLEPVAFEPGA
                     MVLREGESGDDAYLIREGAVDVTRGERALATLGAGAFVGEVSALTGTQRTATVRAHGE
                     VSAFRVRGDDVRAIVKKHQDLVARLESTMQSRHVPRRAVEVQTAPAPDDPTAVLLRDV
                     GGSTYLKVSREALAIFDDIDGERTLRDLVMRHFERSGAIDPAGVFSTIATLQAAGLVT
                     APRVASDEPDARVLRLLDIILAPRLELRDADVLAIQLHRVVGWAFTRAGLAAALAVGG
                     AGLIALLAVFRQSSPGDFGLGGLVVAFLGLLVAGIGHEAAHAIATKAEGRRIGRAGIG
                     LLWFTPVVYVDTSDAWLLPRMRRIRVNAAGPLFNFAFAGACGLAALVLRERPQDVAVW
                     LAILNLVSVAFNLSPLLEFDGYYVLEDLTNTNALRRKALRFVFGDLVARPRRLDSRAE
                     AGLLAYTFGALAYVVVMSVVVLSGVPQLVDGTLAGRIGAEFLPLVGAGIALVLAALLV
                     GPFIGEILAARAAPAD"
     gene            2184..>2825
                     /locus_tag="VFV20_01460"
     CDS             2184..>2825
                     /locus_tag="VFV20_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287046.1"
                     /translation="MRPIAVLYENDAWMAPLFAALDAEGAPVERVFANDLVFDPAGSA
                     PRWSLALNKVSPSSYLRDHARSIRVARDFLPFLETRGVPVVNGSRAFALETSKARQLV
                     LLADLGIRAPAARVVTDVAQIPAAARSLVFPLIVKPNVGGSGALMRRFDTFDDLVARL
                     DTIDLGPDGTGLVQEFHETVDGAIHRVEFLDGEYLYAIKITNGASRDFNLCPAD"
BASE COUNT          439 a         1024 c         1006 g          356 t
ORIGIN      
        1 ggccggtcgc gtgatggacc gcactgctac gggcgtccgg ggccggactt cgcttcggtg
       61 agaacaacgc cctgcgcgtg ccacccgccg ccacgcggac gcgacccgag tctgcggaga
      121 gcacggctag tccgccgggg ccgccctcgc cgccaggatc tcaccgatga atgggccaac
      181 cagcagcgcc gcgagcacca gcgcgatgcc ggcgccgacg agcggcagga actccgcgcc
      241 gatgcgtccg gcgagcgtgc cgtccacgag ctgcggcacg cccgagagca cgacgacgct
      301 catgacgacg acgtacgcga gggcgccgaa cgtgtatgcc agcagcccgg cctcggcccg
      361 cgagtccagt cgccggggcc gcgcgaccag atcgccgaac acgaaccgca gggccttccg
      421 gcgcaaggcg ttcgtgttgg tgaggtcctc cagcacgtag tagccgtcga actcgaggag
      481 gggcgacaga ttgaacgcga ccgagacgag gttcaggatc gcgagccaga cggcgacgtc
      541 ctgggggcgc tcacggagca ccaacgccgc gagaccgcag gcgccggcga acgcgaagtt
      601 gaacagggga cccgccgcgt tgacgcggat gcgccgcatc cgtggcagca gccacgcatc
      661 ggacgtgtcc acgtacacga cgggggtgaa ccagagcagc ccgatgccgg ccctgccgat
      721 ccggcggccc tccgccttgg tggcgatggc gtgcgccgcc tcgtgcccga tccccgcgac
      781 gagcaggccc aggaacgcca ccacgaggcc gcccaggccg aagtcgccgg gcgacgactg
      841 gcggaacacg gcgaggagcg cgatgaggcc ggcaccgccg accgcgagcg ccgccgcgag
      901 gccggcgcgc gtgaaggccc agccgacgac gcggtgcaac tgaatggcga gcacatccgc
      961 atcgcgcagc tccagccgcg gggcgaggat gatgtcgagg agtcgcagca cgcgagcgtc
     1021 gggctcgtcg ctcgcgacgc gcggcgcggt cacgaggccg gccgcctgga gcgtcgcgat
     1081 ggtcgagaag accccggccg ggtcgatcgc accactgcgc tcgaaatgcc gcatcaccag
     1141 gtctcgcagc gttcgctcgc cgtcgatgtc gtcgaagatc gcgagcgcct cccgcgacac
     1201 cttgaggtac gtgctgccgc cgacgtcgcg gagcagcacc gcggtggggt cgtcgggcgc
     1261 gggtgccgtc tggacctcga ccgcacggcg cggaacgtgg cgcgactgca tcgtgctctc
     1321 gagccgcgcg acgaggtcct gatgcttctt cacgatcgcc cggacgtcgt caccgcggac
     1381 gcggaacgcg gacacctctc cgtgcgcgcg gaccgtggcg gtgcgctgcg tgccggtgag
     1441 cgcggagacc tctccgacga acgcaccggc gccgagcgtc gcgagcgcgc gctcgccgcg
     1501 cgtcacgtcc acggcgccct cgcggatgag atacgcgtcg tcgccgctct caccctcgcg
     1561 tagcaccatc gcgccgggct cgaacgcgac cggttccagc ttcgcggcga gcgcctcgat
     1621 cgcggacgca ggcagatccg cgaaggggct cgagcggcgg aggaagttgt tcccgacgcg
     1681 gacccgagcg aagcccgcga gctcgcgccg gaagtctgga cggctcaccg cgagatcgcg
     1741 gatcgccgcg gcggacatcc ggtacgcgcg gacgcgggtc agcgccgtga cggtggcgct
     1801 gcgcgcttcg tcggaaagca ccgccatctc gccgacgacg agcggcgcgc gtggcgaggc
     1861 caccaccgtg ccccgctgcg tgacgcgcac gcgtccgccg acgatcgcgt agacggcatc
     1921 gcccgggtcg ccctcgcgga tcacgtcgct tcccggggcg aacgagacgc gcgtcgcgtc
     1981 cgcgagcaga cgagcacgcg tgacctcgtc gaggaccgcg aatgcgccga agtcgagctc
     2041 cgtgcccacc tggtcgcagg gtagcgaagc gagtgcgggc agagtgcacg gaattcatcc
     2101 ctggagccgc gtgttggcat ttccgagcga tatcctcggg aattgccgga ccgcactacg
     2161 acgcgacaga gagaggggag ccagtgcgac cgatcgccgt cctgtacgag aacgacgcct
     2221 ggatggcgcc gctcttcgcc gccctcgatg ccgagggcgc gccggtggag cgcgtgttcg
     2281 cgaacgacct cgtgttcgat cccgcggggt ccgcgccgcg gtggtcgctc gccctcaaca
     2341 aggtctcgcc ctcctcgtac ctacgcgacc acgcgcggag catccgcgtc gcgagggact
     2401 tcctcccgtt cctcgagacg cgcggcgtgc cggtcgtcaa cggctcgcgg gcattcgcgc
     2461 tggagacgtc gaaggcgcgc cagctcgtcc tcctcgccga cctcggcata cgggcgccgg
     2521 ccgcgcgcgt cgtcacggat gtcgcgcaga tccccgccgc cgcgcgctct ctcgtcttcc
     2581 cgctcatcgt caagccgaac gtcggcggct ccggcgcgct gatgcgccgc ttcgacacgt
     2641 tcgacgacct cgtcgcgcgg ctggacacga tcgacctcgg gccggatggg accgggctgg
     2701 tccaggagtt ccacgagacc gtcgatggcg ccatccatcg ggtcgagttc ctcgacggcg
     2761 agtacctcta cgcgatcaag atcacgaacg gcgcctcgcg ggacttcaac ctgtgcccgg
     2821 cggac
//
LOCUS       DASTZR010000066         5397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_708943, whole genome shotgun sequence.
ACCESSION   DASTZR010000066 DASTZR010000000
VERSION     DASTZR010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708943"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="VFV20_01465"
     CDS             <1..1020
                     /locus_tag="VFV20_01465"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU5287047.1"
                     /translation="GAHAATFSAQCLGGDLGALLDLMAEVLRVPALAADDLEFVRGQT
                     LAQLERDERDTRAVVERVWREHVYPKDHPFHSPGIGDAGVVREATADEIRDYHGTAIR
                     PDGALLVVAGGVTAAAVEEAAGSAFRDWRVTAQRTPRDVQDVAIAGTVRRAVTVPDKT
                     QSDVIIGWPGIPRNDPRFVATRVTNMVYAADTFASRAGNVIRDQLGLAYYVFSGMGSS
                     RGQSPWIVRMGVNPQNVRRAIEVALDELRKVSEGEIADDDLALAKDKLVGELDVALES
                     PGGVASMVLEAELFDLGPDHFERYPKALRAVTKEQVVETARTCISPDRYALVVAGPPL
                     PEPIS"
     gene            1017..1388
                     /locus_tag="VFV20_01470"
     CDS             1017..1388
                     /locus_tag="VFV20_01470"
                     /inference="COORDINATES: protein motif:HMM:NF021525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2255 family protein"
                     /protein_id="HEU5287048.1"
                     /translation="MTGAFPELLLQLFDVTKEIDVETRSEDGAVHRVPIWVVVVDGEV
                     YVRSVRGPRGRWYRELLARRSAVLHVGSRGVEVRPVKVTATETIDEVSAGFWRKYERS
                     ASLFSMLRLSTLHTTLRLEPA"
     gene            1397..1945
                     /gene="mug"
                     /locus_tag="VFV20_01475"
     CDS             1397..1945
                     /gene="mug"
                     /locus_tag="VFV20_01475"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011209368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HEU5287049.1"
                     /translation="MPFRPTREQLEAARGKGVRDVVGPDLDVLFCGINPGLYTAAIGH
                     HFGRPGNRFWKVLHLAGFTPRELSPFEERELLRYGCGVTNLVNVATARADELDDADLV
                     RGAARLTRAVRRWRPKAVAILGMDAYRTAFARRRTAVGPQEARIADAAVWVLPNTSGL
                     QASYQLADLVRLFGALRAATRR"
     gene            complement(1947..2777)
                     /locus_tag="VFV20_01480"
     CDS             complement(1947..2777)
                     /locus_tag="VFV20_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEU5287050.1"
                     /translation="MYTAAGPLRRRLLRWYDRSGRDLPWRRTRDPYAVLVSEVMLQQT
                     QVARVVPAYDAFLRRFPTLRALSRAPLGDVLRAWSGLGYNRRARDLHRIARAVPGALP
                     LTPDALDALPGVGAYTAGAVACFGGGVRTAFADTNIRRVLGRIVLGRIATEREAVEID
                     RELLPRDAARWHHALMDLGATVCVARAPRCPACPVAAVCRSRGRVAAPERRREAAFAT
                     STRRLRGLALRRLVAAPGGVPRARLERDLADARATSVVDGLARDGLVTISRGRVRLPG
                     "
     gene            2924..3394
                     /gene="greA"
                     /locus_tag="VFV20_01485"
     CDS             2924..3394
                     /gene="greA"
                     /locus_tag="VFV20_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016838602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU5287051.1"
                     /translation="MNNLEKPQYVSADGLKKLQAELEQLRTAKRQEVADRIHAAMEFG
                     DYSENSELEQAKNDQAFLEGRIMTLEQTIKNAVVIDETSRHDLVEVGSHVTVEADGRK
                     EKYVIVGSAEAAPAEGKISNESPVGRALMGHRPGDTVKMSVPAGTIEMKIVSIS"
     gene            3391..4998
                     /gene="lysS"
                     /locus_tag="VFV20_01490"
     CDS             3391..4998
                     /gene="lysS"
                     /locus_tag="VFV20_01490"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEU5287052.1"
                     /translation="MSQAHEARFWADEEADLLPTDRPHVIRDSKTPSGEIPISSLRGP
                     IITDALYRTFKSRGLRVRYVFTIDDYDPMDSQSMKAKAGMAEHMGKPFCDIPSPDPAV
                     ASDFARYHASRFLATFEQLGIHPEEIHWMRDLYRSGALDPQIDLVLRGAAAIREIYER
                     VANVKKDERWLPIGVVCEQCGRLGTTEAYDYDGTTVAYECRRDFVDWAEGCGHTSRRS
                     PFKGGAKLYWNLQWCAMWDHFGVTYEEGGKDLLTAGGSRDRADEIYREVWKKEPPAGL
                     IHEFLNVGGKKMSTSRPDEWKALGAAVHEVVTIIPAELVRFLMLRTNPKRHIDFDPTG
                     TYIPKLYDDYDRCADAYLTEPESDPAKTWLLSQTSGDPEAPVFRVRFSIVADWLQIPS
                     IEPFAHAEERKGQPLTAPERSDLERRVALAREWLARWAPAEARFSVLPALPPDIRLSD
                     LQRRYLGEAASLVGVVKDPEAMQNELYEVAKRVGLTQDGKVSREAFSSIYLAFLGKPS
                     GPKAGWLLATLDPAFVKRRLDEASRGR"
     gene            5042..>5397
                     /locus_tag="VFV20_01495"
     CDS             5042..>5397
                     /locus_tag="VFV20_01495"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287053.1"
                     /translation="MRAKRGGEAQAAERAEPSATGMNGKLPVAIIWHMHQPYYRHPQT
                     SEFVLPWVRLHASKDYLHMARLLQEFPRVKANFTMVPSLRDQLEDYARGADDEDTRVT
                     MRTVDGDLTHEEKQHML"
BASE COUNT          852 a         1859 c         1958 g          728 t
ORIGIN      
        1 ggtgcgcatg cggccacctt cagcgcccag tgcctcggcg gcgacctcgg cgccctgctc
       61 gacctgatgg ccgaggtgct gcgcgtcccg gcgctcgccg cggacgacct cgagttcgtc
      121 cgcggccaga cactcgcgca gctggagcgc gacgagcgcg acacgcgggc ggtggtggag
      181 cgcgtctggc gcgagcacgt ctatccgaag gaccacccgt tccactcgcc cggaatcggc
      241 gacgccgggg tcgtccgcga ggcgaccgcc gacgagatcc gcgactacca cggcaccgcg
      301 atccggccgg acggcgcgct cctcgtcgtc gccggcggcg tcacggccgc cgcggtggag
      361 gaggcggccg gcagcgcgtt ccgcgactgg cgcgtcaccg cgcagcggac gccacgtgac
      421 gtgcaggacg tcgcgatcgc cgggaccgta cggcgcgcgg tgaccgtgcc ggacaagacg
      481 cagtcggacg tgatcatcgg ctggcccggg atcccgcgga acgatccgcg cttcgtcgcc
      541 acgcgggtca cgaacatggt gtacgcggcc gacacgttcg cgagccgcgc cggcaacgtc
      601 atccgcgacc agctcggcct cgcctattac gtgttcagcg gcatgggatc gagccgcggc
      661 cagtcgccgt ggatcgtccg catgggcgtg aacccgcaga acgtgcgccg cgccatcgag
      721 gtcgccctcg acgagctgcg gaaggtgagc gagggcgaga tcgccgacga cgacctcgcg
      781 ctcgcgaagg acaagctcgt cggcgagctc gacgtcgcgc tcgagagccc gggcggtgtc
      841 gcgtcgatgg tgctcgaggc ggagctcttc gacctgggtc ccgaccactt cgagcggtat
      901 ccgaaagcgc tccgcgccgt gacgaaggag caggtcgtcg agaccgcgcg cacctgcatc
      961 tcgcccgacc ggtacgcgct ggtggtcgcg gggcccccgc tgccggagcc gatctcttga
     1021 ccggggcctt ccccgagctc ctccttcagc tgttcgacgt caccaaggag atcgacgtcg
     1081 agacgcgttc cgaggatggc gccgtccacc gtgtgcccat ctgggtggtc gtcgtggatg
     1141 gcgaggtgta cgtgcggtcg gtgcgcggcc cgcgcgggcg ctggtaccgc gagctgctcg
     1201 cgcggaggtc ggcggtcctg cacgtaggct cgcgcggcgt cgaggtgcgc ccggtgaagg
     1261 tgaccgcgac cgagacgatc gacgaggtga gcgcgggttt ctggcgcaag tacgagcgca
     1321 gcgcgtcgct gttctcgatg cttcgcctct cgacgctgca cacgaccctc cgcctggagc
     1381 ccgcctgaag agggacgtgc cgttccgccc gacgcgcgag cagctggagg ccgcgcgcgg
     1441 gaagggtgtc cgcgacgtcg ttggcccgga tctcgacgtg ctgttctgcg gcatcaaccc
     1501 cggcctctac accgcggcca tcggtcatca cttcggccgg ccgggaaacc gcttctggaa
     1561 agtgctgcat ctcgcggggt tcacgccgcg agagctctcg ccgttcgagg agcgcgagct
     1621 gctgcgctac ggctgcggcg tgacgaacct ggtgaacgtc gcgaccgcgc gggccgacga
     1681 gctggacgat gccgacctcg tgcgcggcgc tgcccgtctc acgcgcgccg tccgccgctg
     1741 gcggccgaag gccgtcgcga tcctcgggat ggacgcctac cggacggcgt tcgcgcgacg
     1801 ccgcaccgcg gtcggtccgc aggaggcccg catcgccgac gcggcagtct gggtgctgcc
     1861 gaacacgagc ggactgcagg cgagctacca gctcgccgac ctggtgcgcc tcttcggcgc
     1921 gctgcgcgcc gcgacgcgcc gctagatcag ccgggcagcc gcacgcggcc gcgcgagatc
     1981 gtgacgagac cgtcgcgcgc gagtccgtcg acgaccgagg tcgcgcgcgc gtccgcgagg
     2041 tcgcgctcga ggcgcgcccg cggtacgccg cccggcgccg cgacgagccg gcggagcgcc
     2101 agtccgcgca ggcggcgcgt gctcgtggcg aacgccgcct cgcgccgacg ctcgggcgcg
     2161 gcgacccgtc cgcgggagcg gcacacggcg gcgaccgggc aggcgggaca gcgcggcgcg
     2221 cgcgcgacgc agacggtcgc gccgagatcc atgagcgcgt gatgccagcg cgccgcgtcg
     2281 cgcggcagga gctcgcgatc gatctcgacg gcctcgcgct cggtcgcgat gcggccgagg
     2341 acgatccgcc cgaggacccg gcgaatgttc gtgtcggcga aagcggtccg cacgccgccg
     2401 ccgaagcacg cgacggcgcc ggccgtgtag gcgcccacgc cgggcagcgc gtcgagcgcg
     2461 tccggcgtga gcggaagggc gccggggacg gcgcgcgcga tgcggtgcag gtcgcgcgcg
     2521 cggcggttgt atcccaggcc gctccatgca cggagcacgt cgccgagcgg cgcgcgggag
     2581 agcgcgcgga gcgtgggaaa gcggcgcagg aacgcgtcgt aggcgggcac cacgcgggcg
     2641 acctgcgtct gctgcagcat cacctcggag acgaggacgg cgtagggatc gcgcgtgcgg
     2701 cgccacggca ggtctcgtcc ggagcggtcg taccagcgca ggagccgccg cctgagaggg
     2761 ccggccgccg tgtacacttc gtccaacgct ataggaagcc cgaagtgctg agggccgtca
     2821 cggccttcag tgcttttctt ttagggcttg ggggcgggag cccccgcgaa aaatgcaaaa
     2881 ccgatcgtcg cgcgtcacgc gcgccgctcc agacggaggc caaatgaaca atctcgagaa
     2941 gcctcagtac gtgtccgcgg acgggctgaa gaagctccag gccgagctcg agcagctccg
     3001 caccgccaaa cggcaggagg tcgcggaccg gatccacgcc gcgatggagt tcggcgacta
     3061 cagcgagaac tccgagctcg agcaggcgaa gaacgatcag gccttcctcg agggccggat
     3121 catgacgctc gagcagacga tcaagaacgc ggtcgtcatc gacgaaacgt cgaggcacga
     3181 ccttgtggag gtcggcagcc acgtcaccgt ggaggccgat ggccgcaagg agaagtacgt
     3241 catcgtcgga tcggccgagg ccgctccggc cgagggcaag atcagcaacg agtccccggt
     3301 cgggcgcgcg ctcatgggcc accgcccggg cgacaccgtg aagatgtccg tgccggccgg
     3361 caccatcgag atgaagatcg tctccatcag ttgagccagg cgcacgaggc ccggttctgg
     3421 gcggatgagg aggccgacct cctcccgacc gaccgtcccc acgtgatccg cgattcgaag
     3481 acgccgtccg gggagatccc gatctcgtcg ctgcgcggac ccatcatcac cgacgcgctc
     3541 tatcgcacat tcaagtcgcg cggcctgcgc gtgcgctatg tcttcacgat cgacgactac
     3601 gacccgatgg actcgcagtc gatgaaggcg aaggccggca tggccgagca catggggaag
     3661 ccgttctgcg acatcccgtc gcccgaccct gccgtcgcgt ccgatttcgc gcgctatcac
     3721 gcgtcgcgct tcctcgcgac gttcgagcag ctcggcatcc atccggagga gatccactgg
     3781 atgcgcgacc tgtaccgatc gggtgcgctg gatccgcaga tcgacctcgt gctgcggggc
     3841 gccgccgcga tccgcgagat ctacgagcgc gtggcgaacg tgaagaagga cgagcgctgg
     3901 ctgccgatcg gggtcgtgtg cgagcagtgc ggccggctcg gcaccaccga ggcctacgac
     3961 tacgacggca cgacggtcgc gtacgagtgc cggagggact tcgtggactg ggccgagggc
     4021 tgcgggcaca ccagccggcg ctcgccgttc aagggcgggg cgaagctcta ctggaacctc
     4081 cagtggtgcg cgatgtggga ccacttcggc gtcacctacg aggagggcgg gaaggacctg
     4141 ctgaccgcgg gcggatcgcg cgaccgtgcc gacgagatct accgcgaggt ctggaagaag
     4201 gagccgccgg ccggcctgat ccacgagttc ctgaacgtgg gcgggaagaa gatgtccacg
     4261 tcgcggccgg acgagtggaa ggcgctcggt gcggccgtcc atgaggtcgt cacgatcatc
     4321 ccggcggagc tggtgcgctt cctcatgctg cggacgaatc cgaagcgaca catcgacttc
     4381 gatcccaccg gcacctacat cccgaagctc tacgacgatt acgaccggtg cgccgatgcg
     4441 tacctcacgg agcccgagag cgatcccgcg aagacgtggc tcctctccca gacgagcggc
     4501 gatccggagg cgccggtctt ccgcgtgcgc ttctccatcg tcgccgactg gctgcagatc
     4561 ccgagcatcg agccgttcgc ccatgccgaa gagcgcaagg gtcagccgct cacggcgccc
     4621 gagcgcagcg acctcgagcg ccgcgtcgcg ctcgcgcgcg aatggctcgc gcgctgggct
     4681 ccggccgagg cgaggttctc ggtgctgccc gcgctccctc cggacatccg gctcagtgac
     4741 cttcagcgtc gctacctggg cgaggcggcg tcgctcgtcg gcgtggtgaa ggatcccgag
     4801 gcgatgcaga acgagctcta cgaggtcgcg aagcgcgtgg gcctcacgca ggatggaaag
     4861 gtctcgcgcg aggcgttctc gtccatctat ctcgcgttcc tgggaaagcc gagcggaccg
     4921 aaggcgggct ggcttctcgc caccctcgat ccggccttcg tgaagcgccg cctcgacgag
     4981 gcgtcacgag gcagatgaga ggccgagcga ggagcgggtc ccccgcgacg agcgagtccg
     5041 catgcgagcg aagcgaggcg gcgaggcgca ggccgcggag cgggccgagc cgagcgcgac
     5101 aggcatgaac gggaagcttc ccgtcgcgat catctggcac atgcatcagc cctattaccg
     5161 gcatccgcag acgagcgagt tcgtgctgcc gtgggtgcgt ctgcacgcgt cgaaggacta
     5221 cctccacatg gcgcggctcc tccaggagtt cccgcgcgtg aaggcgaact tcacgatggt
     5281 cccgagcctg cgcgaccagc tcgaggacta cgcgcgcggc gccgacgacg aggacacgcg
     5341 cgtgacgatg cgcaccgtcg acggcgatct cacgcacgag gagaagcagc acatgct
//
LOCUS       DASTZR010000067         3437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_422116, whole genome shotgun sequence.
ACCESSION   DASTZR010000067 DASTZR010000000
VERSION     DASTZR010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422116"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..879
                     /locus_tag="VFV20_01500"
     CDS             313..879
                     /locus_tag="VFV20_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020474659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287054.1"
                     /translation="MTDTGQRTTRRTHYVGVFDDRNDAELAARDLREGGFKDDDIGYA
                     WRDDAGKTHQEGNKAGEMAASGAGTGVVLGGIIGAAAALLIPGVGPVVSGGLLASALA
                     GAATGAVAGGVAGGVTGALVGLGIPEDEAKYYDSQLREGRTLMTVRAEDRFDEAGDIV
                     RRRRGYDYTTRGDRPTTTRERIEEDTRR"
     gene            complement(958..1434)
                     /locus_tag="VFV20_01505"
     CDS             complement(958..1434)
                     /locus_tag="VFV20_01505"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEU5287055.1"
                     /translation="MDALSLLTEDHQKVKKLMAEIEKTTERGVKTREELFGKLLTELT
                     IHEQIEEQIFYPEVKEKATTKQLEELVTESYEEHHFVDTVKAEIQDTPFEAEEWAAKF
                     KVMKENVEHHAFDEEEGKMFPKVRKAFSKAELEDMGTRMAELKQQLMENAPEEATR"
     gene            1614..1973
                     /locus_tag="VFV20_01510"
     CDS             1614..1973
                     /locus_tag="VFV20_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5287056.1"
                     /translation="MCEHGPNEPCVLLVDDDPAIRVVVSDLLESEGYHVVAVETREDA
                     RAVITRQPVALMLLDTGVDALASPDRLRFAEPHPPIVVFSAGTDLEVVAARAGAVAWL
                     AKPFETQDLIALVARYS"
     gene            2007..2210
                     /locus_tag="VFV20_01515"
     CDS             2007..2210
                     /locus_tag="VFV20_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287057.1"
                     /translation="MPGIEYVCTARHDGGDRGHDGPTVTVYESRWACCPTGGSDGHAW
                     KRIPPTTVDDMRASARPREQMRT"
     gene            complement(2235..>3437)
                     /locus_tag="VFV20_01520"
     CDS             complement(2235..>3437)
                     /locus_tag="VFV20_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287058.1"
                     /translation="RERVDALLPRVDAAVWVVDPEKYNDDVMHSAYLRRVAPGLRRQL
                     VAMNRSDLLAPQDADRIADDIRAQLGRDGLTGIDVVTTRAREGGAGITALRRWLEEGV
                     QAKRVIASRLAGEARAAVRGLAADAGVTDGTAAALIEPARTARAVEAVARGVLALVDI
                     AALERQAVAATRLAARPRGAGPMGHLTSALYRLTGRARASADPAGYLRRWQLRGSLAP
                     AVEPLRELIASMIPGVPAPLRGALATLSTPAAVEQRLSKVVDGVLAAEAAEFRVPTSA
                     LWTLIGAGQYAVTATILFCALWLASLFVIHDAPVGSIDVPYLGAVPTPVALLAGALLV
                     GYLLALTLRWHAGWLGRRWARRIGARITREIRERVADSMLLPVEQLDASRARLAATVR
                     AAEDCTGE"
BASE COUNT          523 a         1205 c         1184 g          525 t
ORIGIN      
        1 acggcaatgt cgacacgaag gtcctctttg gtaatgccgg cctcctggcg cgattcatca
       61 cggactgcgt gctcgaacac ttcgacgtgg ccgtcccacg acgccgaggc gttcgtcagt
      121 aggaccgccg gcgggccgca gcatcgagaa acgcgtccgc tcgcttgacc gcggtcgcac
      181 atcatccggg ctattcggtg gcgcctcgct cgtccagcgc gcgcgtactc atcgccccga
      241 acgctgttac gaaccaagaa gtctggcttg gaacgtgcgt gacttggcgc cacatagcgc
      301 aggaggaatc caatgaccga tacaggacag cgaacaacca ggcgaaccca ctacgtcggc
      361 gtcttcgacg accgtaacga cgccgaactc gccgctcgcg accttcgcga gggcgggttc
      421 aaggacgacg acatcgggta cgcgtggcgt gacgatgccg gcaagacgca tcaagagggc
      481 aacaaggccg gggaaatggc cgcgtccggc gcgggcaccg gcgtggtgct cggcgggatc
      541 atcggcgcgg ctgccgcgct cctcatcccg ggcgtcggcc cggtggtgtc gggtggtctg
      601 ctcgcgagcg cgctcgcggg cgcggcgacc ggagccgtgg ccggcggtgt cgccggcggc
      661 gtcaccggag cgctcgtcgg gctcgggatc cccgaggacg aggcgaagta ctacgacagc
      721 caactgcgcg agggccgcac gctcatgacg gtccgcgcgg aagaccgctt tgacgaggcc
      781 ggcgacatcg tccgccgccg tcggggctat gactacacga cgcgcggcga ccgcccgacc
      841 acgacacgcg agcggatcga ggaagacacg cgacgctagc gaacgtctga gtcgcgtcaa
      901 agagaaggcc cgccgtgtgg cgggccttct cgttcgttcg ttcgcgcgga tcgcgcgtca
      961 gcgcgtggct tcctcagggg cgttctccat gagctgctgc ttcagctccg ccatgcgggt
     1021 gcccatgtcc tcgagctccg ccttgctgaa cgccttgcgc accttcggga acatcttgcc
     1081 ctcttcctca tcgaaggcgt gatgctcgac gttctccttc atgaccttga acttcgcggc
     1141 ccactcctcc gcctcgaatg gcgtgtcctg gatctcggcc ttcacggtgt cgacgaagtg
     1201 atgctcctcg tatgactcgg tcacgagctc ctcgagctgc ttcgtcgtcg ccttctcctt
     1261 gacctcggga tagaagatct gctcctcgat ctgctcgtgg atggtcagct ccgtgagcag
     1321 cttgccaaag agctcttcgc gcgtcttcac gccgcgctcg gtcgtcttct cgatctcggc
     1381 catgagcttc ttgactttct gatggtcctc tgtgagcaaa ctcagtgcgt ccacatgtaa
     1441 ctccctcgtg ttctcagcgg tcgtcagagc acggggggtg ccaccgatgc ggctcagccg
     1501 ccgtgggatc gctccgctgt tccctgagaa tgtgcgcaag cgcctggacg gtggcggccc
     1561 gttcccgcaa ttgcgtgatg cgaccacgcg cgatcgggac gacgctagag cgcatgtgtg
     1621 agcacgggcc gaacgagcca tgtgttctgc tcgtcgatga cgacccggcg atccgcgtgg
     1681 tcgtcagcga ccttctcgag agcgagggct accacgtggt cgctgtcgag acgagggagg
     1741 atgctcgcgc cgtcatcacg aggcagccgg tcgcattgat gctcctcgat accggcgtcg
     1801 acgcgctggc atcccccgat cggctgcgct tcgcggagcc gcacccgccg atcgtggtct
     1861 tcagcgccgg tacggacctg gaggtagtcg ccgcacgggc aggcgcggtc gcgtggcttg
     1921 cgaagccgtt cgagacgcag gatctcatcg cgctcgttgc gcggtactcg taggtacgcg
     1981 gcagcgcgag acaggggagg catcggatgc cgggcatcga gtacgtctgc accgcacgtc
     2041 acgacggcgg cgaccgcggt cacgatggac caacggtcac cgtgtacgag tcgcgctggg
     2101 catgctgtcc gacgggcggg tcggacggac atgcgtggaa gcgcatcccc ccgacgacgg
     2161 tcgacgacat gcgcgcctcg gcgcgcccgc gcgagcagat gcggacctag ccgcgcgagc
     2221 cggcggtcgt gcgtctactc gccggtgcag tcctccgccg cacggaccgt cgccgcgagc
     2281 cgcgcgcgcg acgcgtcgag ctgctcgacg ggcagcagca tgctgtccgc gacccgctcc
     2341 cggatctcgc gcgtgatccg cgcgccgatc cgccgggccc atcgccgacc gagccagccc
     2401 gcgtgccatc gcagcgtcaa cgcgagcaga tagccgacga gtagcgcccc ggcgagcagc
     2461 gcgacgggcg tcggcacggc cccgaggtac ggcacatcga tcgaacccac cggcgcgtcg
     2521 tggatgacga agagggaagc gagccacagc gcgcagaaga ggatcgtggc ggtgaccgcg
     2581 tattgccccg cgccgatgag cgtccagagc gcgctcgtcg gaacgcggaa ctccgccgcc
     2641 tccgccgcga gcacgccatc caccaccttc gaaagccgct gctcgacggc cgccggcgtg
     2701 ctgagggtcg cgagcgcgcc ccggagcggg gcgggcacgc ccgggatcat cgacgcgatg
     2761 agctcgcgca gcggctcgac ggcgggcgcg agcgagccgc gcagctgcca ccgccgcagg
     2821 tacccggccg gatccgccga cgcgcgagcg cggccggtga gccgataaag agcgctcgtg
     2881 aggtggccca tgggtcccgc accccgcggc cgcgcggcga gacgcgtggc ggccaccgcc
     2941 tggcgctcga gggcagcgat atccaccagc gcgagcacac cgcgagcgac cgcctcgacc
     3001 gcgcgggccg ttcgagccgg ctcgatgagc gccgctgccg tcccatccgt cacgccggca
     3061 tccgccgcga ggccccggac ggcggcccgt gcctcgccgg cgaggcgcga cgcgatcacg
     3121 cgcttcgcct ggactccctc ctcgagccag cgccgcagcg cggtgatccc cgccccgccc
     3181 tcgcgcgcgc gtgtcgtgac gacgtcgatg ccggtgaggc catcgcgccc gagctgcgct
     3241 ctgatgtcgt cggcgatccg gtccgcgtcc tggggagcga ggaggtcgct ccgattcatg
     3301 gccacgagct ggcgccgcag gcccggcgcg acgcgccgca gataggcgct gtgcatgacg
     3361 tcgtcgttgt atttctcggg atcgacgacc cacacggccg cgtcgacgcg gggaaggagc
     3421 gcgtcgacgc gctcgcg
//
LOCUS       DASTZR010000068         2190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1204483, whole genome shotgun sequence.
ACCESSION   DASTZR010000068 DASTZR010000000
VERSION     DASTZR010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204483"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VFV20_01525"
     CDS             <1..183
                     /locus_tag="VFV20_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5287059.1"
                     /translation="VLSKIAPMRYAVDLVRDIFYAGGPPIPLADLPTNLGIIAAEFVV
                     FMVVGTAGFVRAERNR"
     gene            207..461
                     /locus_tag="VFV20_01530"
     CDS             207..461
                     /locus_tag="VFV20_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287060.1"
                     /translation="MPLRRLKKTARTIDAALRDAHKARDAHSDPTFDRDLQGDRRATL
                     RKFDTVEHALEDREKAERAKAGAKPGPGASKATKAKAKKK"
     gene            569..1342
                     /locus_tag="VFV20_01535"
     CDS             569..1342
                     /locus_tag="VFV20_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5287061.1"
                     /translation="MPAPHRDPDLVAHGYAYVGHTGIFRVAEAEGRLAALACAIVRDG
                     IWFLSGFWTDPARRLQGIGGPLLREVWDEGRRRGARSFYVWSSPDFPAIATYLKLGML
                     PGTQLFAFAGAAQTAPTDRAFRVEPLSAARVADLDHALVGVRRDGDHEYWLARDGARG
                     RVVVNGGDLVGYYYVHEGTIGPVGWTTESAGEQVLRLAIGDASEAGRDVQLTAPGTNH
                     TAIRVALGSGLRLIRASHLLWTEPIGAMERYLPSGPLLF"
     gene            complement(1391..>2190)
                     /locus_tag="VFV20_01540"
     CDS             complement(1391..>2190)
                     /locus_tag="VFV20_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287062.1"
                     /translation="LPLVLAVVLVELAAGGAFLVWLLDRRGIAPGGFLKLTAGVDAVC
                     GAAAVGLIPTIIPRELQSQMGIDAGPMSSFGQALVVFLVLLVIQFALTFVPVKDLRAV
                     AGALTVVVGVATLEIAAIARPTQSPFDIFALAALPLGALALGGSDAAMLLGHWYLVTP
                     KLSTGPLRSAALIVVAAVALQLGVVAVSFARGDLAGSTSEVGLTVAIGIRIGVGLLMT
                     LAVALGGWWTARMNTQSSTGLLYVGLGTALAGEISARVLFFLTGAAI"
BASE COUNT          339 a          786 c          780 g          285 t
ORIGIN      
        1 gtgctctcga agatcgcgcc gatgcggtat gccgtcgacc tggtgcgcga catcttctac
       61 gcgggaggcc cgcccatccc gctcgccgac ctgccgacca acctcggcat catcgcggcc
      121 gaattcgtcg tcttcatggt cgtgggcacg gccggtttcg tccgcgcgga gcgcaatcgc
      181 tagctccggc tcaatacaat cagcccatgc ccctacgcag gctcaagaag acggcacgga
      241 cgatcgacgc ggcgctacgg gacgctcaca aggcgcggga cgcgcacagc gatccgacct
      301 tcgatcgcga tctccagggc gaccgtcgcg ccacactgcg caagttcgac acggtcgaac
      361 acgcgctcga ggatcgcgag aaggccgagc gggcgaaggc cggtgcgaag cccggcccgg
      421 gagcgagcaa ggcgacgaag gcgaaggcga agaagaaata ggcgcgcgcc ccgagggcgt
      481 cgtctatcgc ccgatccgcg aggacgaatt caccgctgcc gccgacctgt tcttcgacgc
      541 gctcgccgac ctcaccgcgc gcctgggcat gccggcgccg caccgcgacc cggacctggt
      601 cgcgcacggg tacgcctacg tggggcacac cgggatcttc cgcgtcgccg aggccgaggg
      661 acgtctcgcg gcgctcgcgt gcgcgatcgt gcgcgacggc atctggttcc tctccggctt
      721 ctggaccgac cccgcccgcc ggctgcaggg catcggcggc ccgctccttc gcgaagtctg
      781 ggacgagggc cggcgccgcg gcgcccggtc cttctacgtg tggtcatccc cggacttccc
      841 ggccatcgct acgtacctga agctcgggat gcttcccggc acgcagttgt tcgcgttcgc
      901 cggcgccgcg cagaccgctc cgacggatcg tgccttccgc gtggagccgc tgtcggccgc
      961 gcgcgtcgcg gatctcgatc atgctctcgt cggcgttcgg cgcgacgggg atcacgagta
     1021 ctggctcgcg cgcgacggcg cacgcggtcg cgtcgtcgtg aacggaggtg acctcgtcgg
     1081 ctactactac gtgcacgagg gcaccatcgg accggtgggg tggacgaccg agagcgccgg
     1141 cgagcaggtg ctccgtctcg cgatcggcga cgcgagcgag gccgggcgtg acgtgcagct
     1201 gacggcgccg gggacgaatc acaccgcgat ccgcgtcgcg cttggctccg ggctgcgtct
     1261 catccgcgcg tcgcacctgc tctggacgga gccgatcggc gcgatggagc gctacctccc
     1321 ctcgggcccg ctgctgttct agtcttcgcg ctcggctcgg cccgctgcgg ggacctcctc
     1381 ccgcggtctg ctagatcgcg gcgccggtga ggaagaacag cacgcgcgcg ctgatctcgc
     1441 cggcgagcgc ggtgccgagg ccgacgtaga gcaggccggt cgacgactgc gtgttcatgc
     1501 gcgccgtcca ccagccgccg agcgcgaccg cgagcgtcat gaggagcccg acgccgatgc
     1561 ggatgccgat cgccacggtg agacccacct ccgaggtcga gccggcgagg tcgccgcgcg
     1621 cgaaggagac cgcgacgacg ccaagctgga gcgcgaccgc ggcgacgacg atgagcgcgg
     1681 cggaccggag cggacccgtg ctgagctttg gcgtgacgag gtaccagtgg ccgagcagca
     1741 tcgcggcgtc cgatccgccg agcgcgagcg caccgagcgg gagcgccgcg agcgcgaaga
     1801 tgtcgaaggg cgactgcgtc ggacgggcga tcgcggcgat ctcgagcgtc gcgacgccga
     1861 cgaccaccgt gagcgcgccg gcgaccgcgc gcaggtcctt caccggcacg aaggtcagcg
     1921 cgaactggat gaccaggagc acgaggaaca cgaccagggc ctgtccgaac gacgacatcg
     1981 ggccggcgtc gatccccatc tggctttgca gctcgcgcgg gatgatcgtc gggatgagtc
     2041 cgaccgccgc cgcgccgcac accgcgtcga ccccggccgt gagcttcagg aagccgcccg
     2101 gcgcgatgcc gcgccgatcg agcagccaca ccaggaacgc gccgccggcc gcgagctcga
     2161 cgaggacgac ggccagcacc agcggcagcg
//
LOCUS       DASTZR010000069         3580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_265487, whole genome shotgun sequence.
ACCESSION   DASTZR010000069 DASTZR010000000
VERSION     DASTZR010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265487"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..841
                     /locus_tag="VFV20_01545"
     CDS             71..841
                     /locus_tag="VFV20_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5287063.1"
                     /translation="MSEEVILRLDHVVKRFGGLVAVNDVSFDVKKGEVFALIGPNGAG
                     KTTVFNGVTGLLPPTSGSVFFEGREIAGTKPHKVAELGIARTFQNIRLFEYMTALENI
                     LVGEHCRMGAKLWDSLFKTPRERREERAVTERAMELLTFVGIDRWANSYARNLPYGGQ
                     RRLEIARALATQPRLLLLDEPAAGFTPQEKVELMRLVGKILESGVTVFLIEHDMKVVM
                     GISKRIVVLDHGELIAQGTPDEVRRDARVIEAYLGKQH"
     gene            841..1557
                     /locus_tag="VFV20_01550"
     CDS             841..1557
                     /locus_tag="VFV20_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5287064.1"
                     /translation="MADALLELHGVDAFYGRVQALHGVSIAVGASEIVALIGSNGAGK
                     TTTLRTISGLMHPANGTIRFGERDITHMPAHEIVGLGICQSPEGRRLFARMQVIDNLR
                     MGAYTRRDKPGIQRDMERVFTLFPRLKERSSQLAGTLSGGEQQMLAIGRAMMSSPKLL
                     MLDEPSLGLAPILVDAIFKTILEINQAGTPILLVEQNAVRALEVAHRGYVLETGNIVQ
                     TGTGKELLGSPDVQRAYLGM"
     gene            1554..2099
                     /locus_tag="VFV20_01555"
     CDS             1554..2099
                     /locus_tag="VFV20_01555"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5287065.1"
                     /translation="MTGTTGVAPFFAGWRLANDAIVTAIAPLSAEQLALPVGSPTWPI
                     WASVSHLAGTRVYWLCHVFGEPGAGTTPFTDPNLGWEDDLAHPRGADELVGALGSTFA
                     IIERTLERWTPESLAQTARRVRPDGQVQIHTRQSVLWRRITHDAYHAGEVALTLGANG
                     IPGTSPNGPIDLWSGLSRTAE"
     gene            2147..3517
                     /locus_tag="VFV20_01560"
     CDS             2147..3517
                     /locus_tag="VFV20_01560"
                     /inference="COORDINATES: protein motif:HMM:NF025112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU5287066.1"
                     /translation="MSGELHLAVPPDVEKVVDTLIDAGHDAYVVGGSVRDALRGVDPH
                     DWDVTTDATPQQIQAVFRHSLYNNRFGTVVVRSGQHEVEVTTYRTEAEYTDHRRPDAV
                     AFTASLQEDLQRRDFTMNAMAWRPARGGRPGELVDPFGGRRDLEAHVLRAVGDPNERF
                     REDALRILRAVRFATLLDMRIEPATADAIRRNAELTASLSGERIQQEIVKMLWAPRPS
                     AAFEMLSDLGVLAVICPELEDCKSTPQEKAAAENVFQHSLATLDAAASAKPPGDLVLR
                     LAGLFHDIGKPETFADGHFHQHEFVGEAKARAILRRWKFDKDTVSEVAHLIRHHMFWY
                     QTEWTGSAVRRFIRKVGLDDIPALFALRRADNIGSGARSPRMYALDSLWQRVEEEIHA
                     ANALSTRDLAIDGNDIMGSLGLPAGREIGRILNALFERVLDDPALNTREALLELAKRE
                     HEKPSS"
BASE COUNT          571 a         1274 c         1232 g          503 t
ORIGIN      
        1 gcggttcacc cgcgacgagc gagaccacca gccctgagcg ggtctcctcg gcgcagccga
       61 gagattggac atgagcgaag aggtcatcct ccgcctcgat cacgtcgtga agcgcttcgg
      121 cggcctcgtc gcggtgaacg acgtctcgtt cgacgtgaag aagggcgagg tcttcgcgct
      181 catcggcccc aacggggcgg gcaagacgac ggtcttcaac ggcgtcaccg gcctcctgcc
      241 cccgaccagc ggctcggtct tcttcgaggg gcgcgagatc gcgggcacga agccgcacaa
      301 ggtcgccgag ctggggatcg cacggacgtt ccagaacatc cggctgttcg agtacatgac
      361 cgcgctcgag aacatcctcg tcggcgagca ctgccgcatg ggggccaagc tctgggactc
      421 cctgttcaag acgccgcgcg agcggcgcga ggagcgcgcg gtcaccgagc gcgcgatgga
      481 gctgctgacg ttcgtgggca tcgaccggtg ggcgaacagc tacgcgcgca acctcccgta
      541 cggcggccag cgccgcctcg agatcgcccg tgccctcgcg acgcagcccc gcctgctgct
      601 cctcgacgag cctgccgcgg ggttcacgcc gcaggagaag gtcgagctca tgcgcctcgt
      661 cgggaagatc ctggagtcgg gcgtcacggt ctttctgatc gagcacgaca tgaaggtcgt
      721 catgggcatc agcaagcgga tcgtcgtgct cgaccacgga gagctcatcg cccagggcac
      781 gcccgacgag gtgcgccgcg acgcgcgcgt catcgaggcc tatctcggga agcagcacta
      841 gtggcggacg cgctcctcga gctccacggc gtcgacgcgt tctacgggcg cgtgcaggcg
      901 ctgcatggcg tgtcgatcgc ggtgggcgca tccgagatcg tggccctcat cggcagcaac
      961 ggcgcgggca agacgacgac gctccgcacg atctccggcc tcatgcatcc ggcgaacggg
     1021 acgatccgat tcggcgagcg cgacatcacg cacatgccgg cgcacgagat cgtcggcctg
     1081 ggcatctgcc agtcgccgga gggccgccgc ctgttcgcgc gcatgcaggt catcgacaac
     1141 ctgcgcatgg gcgcgtacac gcgcagggac aagccgggga tccagcggga catggagcgc
     1201 gtgttcacgc tcttcccccg cctcaaggag cgcagcagcc agctcgcggg aacgctctcg
     1261 ggcggcgagc agcagatgct cgcgatcggc cgcgcgatga tgagcagccc gaagctcctc
     1321 atgctcgacg agccgtcgct cggcctcgcg ccgatcctcg tcgacgcgat cttcaagacg
     1381 atcctcgaga tcaaccaggc cgggacgccg atcctgctgg tggagcagaa cgccgtccgc
     1441 gccctcgagg tcgcgcaccg cgggtacgtg ctcgagaccg gcaacatcgt gcagaccggc
     1501 accggcaagg agctgctcgg ctcgcccgac gtgcaacgcg cgtacctcgg gatgtgaccg
     1561 gcaccacagg cgtcgcgccc ttcttcgccg gctggcggct cgcgaacgac gccatcgtca
     1621 ccgcgatcgc gccgctctcc gccgagcagc tcgcgctccc ggtcgggtcc cccacgtggc
     1681 ccatctgggc gagcgtctcg cacctcgccg gcacgcgcgt gtactggctc tgccacgtgt
     1741 tcggcgagcc cggcgccggc acgacgccgt tcacggaccc gaacctcggc tgggaggacg
     1801 acctcgcgca tccccgcggc gccgacgagc tcgtgggcgc gctcgggtcc acgttcgcga
     1861 tcatcgagcg cacgctggag aggtggacgc ccgagtcgct cgcccagacc gcgcgacgcg
     1921 tccggccgga cgggcaggtc cagatccaca cgcgccagtc ggtgctctgg cgcaggatca
     1981 cgcacgacgc gtatcacgcc ggcgaggtcg cactgacgct cggcgcgaac ggcatcccgg
     2041 ggacgagccc gaacgggccg atcgacctct ggagcgggct ctcccgcacc gccgagtagc
     2101 ccgcgcatca gcgggctgtt cggcggcgga tgcctagact cgcttcgtgt ccggagagct
     2161 gcatctggcg gtcccgcccg acgtcgagaa ggtcgtcgac acgctgatcg acgccggtca
     2221 cgacgcctac gtcgtcggtg ggtcggtgcg cgacgcgctg cgcggtgtgg acccgcacga
     2281 ctgggacgtg acgacggacg cgacaccgca gcagatccag gccgtcttca ggcactcgct
     2341 gtacaacaac cgcttcggca cggtcgtcgt gcgctccggt cagcacgagg tcgaggtgac
     2401 gacctatcgc accgaggccg agtacaccga ccatcgcagg ccggacgcgg tcgcgttcac
     2461 ggcgtcgctc caggaggacc tccagcgacg tgacttcacg atgaacgcca tggcctggcg
     2521 cccggcccgg ggcggcaggc cgggcgagct cgtggacccc ttcggcggac ggcgggacct
     2581 cgaagcgcac gtcctgcgcg cggtgggcga cccgaacgag cgcttccgcg aggacgcgct
     2641 gcgcatcctg cgggcagtgc gcttcgcgac gctcctcgac atgcgcatcg agccggcgac
     2701 ggccgacgcg atccggcgca acgccgagct caccgcgtcg ctctcgggcg agcgcatcca
     2761 gcaggagatc gtgaagatgc tctgggcgcc acgcccgtcg gccgccttcg agatgctctc
     2821 cgacctcggt gtgctcgcgg tcatctgccc cgagctcgag gactgcaaga gcacgccaca
     2881 ggagaaggcc gcggcggaga acgtgttcca gcactcgctg gcgacgctgg acgccgccgc
     2941 gagcgcgaag ccgccaggcg acctcgtgct ccggctcgcc ggcctgttcc acgacatcgg
     3001 gaagcccgag acgttcgcgg acggccactt ccaccagcat gagttcgtcg gggaggcgaa
     3061 ggcgcgcgcg atcctgcgcc gctggaagtt cgacaaggac acggtgagcg aggtcgccca
     3121 cctcatccgg catcacatgt tctggtacca gaccgagtgg accggctccg cggtgcggcg
     3181 cttcatccgc aaggtgggcc tcgacgacat cccggcactc ttcgcgctcc gccgggccga
     3241 caacatcggg agcggtgcgc gctcaccgcg gatgtatgcc ctcgactcgc tgtggcagcg
     3301 cgtcgaagag gagatccatg ccgcgaacgc gctctcgacg cgcgacctcg cgatcgatgg
     3361 caacgacatc atggggtcgc tggggcttcc ggcgggtcgc gagatcggcc ggatcctgaa
     3421 cgcgctcttc gagcgcgtcc tcgacgatcc cgccctcaac acacgcgagg cgcttctcga
     3481 actcgcgaag cgcgagcacg agaagccctc gagctgatcc ggtccgacct cgggctaggt
     3541 gcgctcgtac gcggtgggtg cggccgcccc gtacgtggtg
//
LOCUS       DASTZR010000070         3839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_892368, whole genome shotgun sequence.
ACCESSION   DASTZR010000070 DASTZR010000000
VERSION     DASTZR010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892368"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VFV20_01565"
     CDS             <1..424
                     /locus_tag="VFV20_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287067.1"
                     /translation="VIESLGLKIWMSPWSNRFMDWALMKDPDALGPVYESIPGDTDII
                     VSHQPPFGYGDLEETAPGTLAHVGSYELLLAIDRVRPRMVICGHIHRAFGTYEYSGIP
                     IYNVAVADEFYRPTHPLTEIEFAPGRPATVRATHVQPV"
     gene            432..2411
                     /locus_tag="VFV20_01570"
     CDS             432..2411
                     /locus_tag="VFV20_01570"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU5287068.1"
                     /translation="MADLDFLSTIPIFRFFTRAELEEAEKLFREIAFGRDDVVVKLGD
                     PGDTFYVVLDGELEVWDGSTPPRQTGSLRRGDYFGEMALLQGGKRTATVKVARRARLL
                     SLDKPGFDQLFLTNPKAIEYFARVLSQRLAGVTRGQSIRRATTTVSVASRKGLTGETL
                     VAYTLAAILKDITETEVIYVEVRPGQEANPKLLRLLADDLNAVPQKFSEDLVHQSAGP
                     TVLKIAVPPNLTAQAYGEAVSNLVSKLSDTFSFIVFDLGSGTAGLIQSASDYSDVFVS
                     IVDDMSEESGLAAPRSTRVFRVANLYNATTRPVAISSCEPFVLPYESALGAPTREAAA
                     YVRGNPRSAAGLPLYRLAHKLLGTSIGLALGGGAAFGLAHLGVLQVLERAGVPIDMVS
                     GCSQGSIVSVGYAAGLSVEEMIDIARQLGIKRNFLFASDPTFFTKPGILSGQRFLTMM
                     RPYLRGKESFEQLLIPCRTVATDIETGEAVQMGTGHLETAFRASSSVPMVMAPIRVGD
                     RVLVDGGVADPVPLRAIKDMGADLALAVNVVPPMKRGVETALSYWMRRLNVFNPLSYI
                     SDGQDLPNLFDIVMNSMQILQYELGNFKAITADVLINPDLSDFTWIEYYRADELIERG
                     VEAAERALPMIEKQIQARLDPIRQAAPSNRAATGT"
     gene            2414..>3839
                     /locus_tag="VFV20_01575"
     CDS             2414..>3839
                     /locus_tag="VFV20_01575"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEU5287069.1"
                     /translation="MSPLLISVAATALVWAAVAAVYIVLLRKRVLARFSRGFLYSMLL
                     AMVGVALMSSTIIGVWGYKSAQQLLEEEVIEELADIGSIVETELANQRNNVHRQLSAF
                     GTSLVPLLDKGASSTELSAKLHTALSLNDRFLELHVFDEQGRTVASSSRLETTEPISR
                     QAIGANLDGQPFVSEAEKSKSFGREVLFISEPLKDASGAVRGAIGTRFDLQDELKDLV
                     GGIKFNQSGYASMVDGEGQVIAHPDEQRLERVVSHYPAVKAAWLSGGTGSLVADNDRG
                     QHKLFVYRAVENPATLPRQKWVLLTEIDAAELVAPVKKLRDELFVGILLLLIISVVIG
                     HQVSYSVDKPLQELGAFAHRIGTGDLTGRATVSGHDVAGRLAASLNEMAAGLQERDHV
                     KEVFGRYIATQVSDQILSGQVNLGGEARVVTILFSDIRNFTGMSEQMTPQQVVAFLND
                     YFSEMVDAVFEHHGVLDKFMGDGLM"
BASE COUNT          641 a         1315 c         1264 g          619 t
ORIGIN      
        1 ggtaatcgaa tcgctcgggc tgaagatctg gatgtctccc tggtcgaacc ggttcatgga
       61 ctgggcgctc atgaaggatc ccgacgccct cggccccgtc tacgagtcga tccccgggga
      121 cacggatatc atcgtcagcc accagccgcc gttcgggtac ggcgatctgg aagagacggc
      181 gcccggcacg ctcgcgcacg tgggatcgta cgagctgctc ctcgctatcg accgcgtccg
      241 gccgcggatg gtgatctgcg gccacatcca ccgcgcgttc ggcacgtacg aatacagcgg
      301 tatcccgatc tacaacgtcg cggtcgccga cgagttctac cgtccgacgc atccgttgac
      361 ggaaatcgag tttgcgccgg gccgcccggc caccgtccgc gccacgcacg tgcaaccggt
      421 gtgaccgaac catggccgac ctggactttc tctccacgat tcccatcttt cggttcttca
      481 cgcgggcgga gctcgaggaa gcggagaagc tctttcgcga gatcgcgttc ggcagggacg
      541 acgtcgtcgt caagctgggc gacccggggg acacgttcta cgtcgtgctc gacggcgagc
      601 tggaggtgtg ggacggcagc acgcccccgc gccagaccgg ctcgctgcgc cgcggcgact
      661 acttcggcga gatggcgctg ctgcaggggg gcaagcgcac cgccacggtg aaagtggcgc
      721 gccgcgcgcg cctgctctcg ctcgacaagc cggggttcga tcagctcttc ctgaccaacc
      781 cgaaagccat cgagtacttc gcgcgcgtcc tctcgcagcg cctcgccggc gtgacgcggg
      841 gccagagcat ccggcgcgcg acgacgaccg tcagcgtcgc gagccgcaag ggactcaccg
      901 gggaaacgct cgtggcgtac accctcgcgg cgatcctcaa ggacatcacc gagaccgagg
      961 tgatctacgt cgaggtccgg cccggacagg aagccaaccc gaagctcctc cggctgctcg
     1021 ccgacgacct gaacgccgtc ccgcagaagt tcagcgagga cctcgtccac cagagcgcgg
     1081 ggccgacggt gctcaagatc gccgtccccc cgaacctcac cgcgcaggcg tacggcgagg
     1141 cggtctcgaa cctggtctcg aagttgagcg acacgttcag cttcatcgtg ttcgacctcg
     1201 gctccggcac ggcgggcctg atccagagcg ccagcgatta ttcggacgtc ttcgtgtcga
     1261 tcgtggacga catgagcgag gagtccggcc tcgccgcgcc gcgatcgacg cgcgtgttcc
     1321 gcgtcgcgaa cctgtacaac gcgacgacgc gacccgtcgc gatcagcagc tgcgagccgt
     1381 tcgtccttcc gtacgaatcg gcgctcgggg cgccgacgcg cgaagcggcc gcgtacgtcc
     1441 gcggcaaccc gcgatcggcc gcgggccttc cgctgtaccg gctcgcgcac aagctcctcg
     1501 gcacctcgat cgggctcgcg ctcggcggcg gcgcggcgtt cggcctcgcg cacctcggcg
     1561 tgctgcaggt gctcgagcgc gccggcgtgc ccatcgacat ggtgtccggc tgcagccagg
     1621 ggtcgatcgt gtcggtcggg tacgcggcgg gcctcagcgt cgaggagatg atcgacatcg
     1681 cgcggcagct cggcatcaag cgcaacttcc tcttcgcgtc cgatccgacg ttcttcacca
     1741 agcccggcat cctctcggga cagcggttcc tgacgatgat gcgcccgtat ctgaggggga
     1801 aggaatcgtt cgagcagctg ctgattccct gccggacggt cgccaccgac atcgagaccg
     1861 gcgaagcggt gcagatgggg acggggcacc tcgagaccgc gttccgggcg agctcctcgg
     1921 tgccgatggt gatggcgccc atccgcgtgg gcgaccgcgt gctcgtggac gggggcgtcg
     1981 ccgatccggt gccgctgcgc gccatcaagg acatgggcgc cgacctcgcg ctcgccgtca
     2041 acgtggtgcc cccgatgaag cgcggcgtcg agaccgcgct ctcctactgg atgcggcggc
     2101 tcaacgtctt caacccgctc tcgtacatca gcgacggcca ggacctgccg aacctgttcg
     2161 acatcgtcat gaactcgatg cagatcctcc agtacgagct gggcaacttc aaggcgatca
     2221 ccgcggacgt cctcatcaac cccgatctct cggacttcac ctggatcgag tattaccgtg
     2281 ccgacgagct catcgagcgc ggcgtggaag cggcggaacg cgcgctgccc atgatcgaga
     2341 agcagatcca ggcgaggctc gatccgatcc gtcaggccgc gccctccaac cgcgcggcaa
     2401 ctggtacctg atcatgagtc ccctgctgat ttcggtggcc gcgacggcgc tcgtctgggc
     2461 ggcggtggcg gccgtgtaca tcgtgctgct gcgcaagcgc gtcctcgcgc gattctcgcg
     2521 cggcttcctc tattcgatgc tcctcgcgat ggtcggcgtc gcgctgatgt cgtccacgat
     2581 catcggcgtc tggggctaca agtccgcgca gcagctgctc gaggaggaag tcatcgagga
     2641 gctcgccgac atcggcagca tcgtcgagac ggagctcgcc aaccagcgca acaacgtcca
     2701 ccggcagctc tcggcgttcg gcacgtcgct cgtgcccctc ctggacaaag gcgcgtcctc
     2761 gaccgagctg agcgcgaagc tccacaccgc gctgagcctc aacgatcgct tcctcgagct
     2821 gcacgtcttc gacgagcagg ggcggaccgt cgcgtcgagc agccgtctcg agacgaccga
     2881 acccatcagc cggcaggcga tcggcgcgaa cctcgacggg cagcccttcg tctccgaggc
     2941 cgagaagtcc aagagcttcg gacgcgaggt cctcttcatc agcgagccgc tgaaggacgc
     3001 gagcggcgcc gtgcgggggg ccatcgggac gcggttcgat ctgcaggacg agctgaagga
     3061 cctcgtcggg ggcatcaagt tcaaccagag cggatacgcc tccatggtgg acggcgaggg
     3121 acaggtgatc gcacatccgg acgagcagcg gctcgaacgc gtggtgtcgc actatccggc
     3181 cgtcaaggcg gcgtggctgt cgggaggcac agggtcgctc gtcgcggaca acgatcgcgg
     3241 ccagcacaag ctgttcgtgt atcgtgccgt cgagaatcct gccacgctgc cccggcagaa
     3301 gtgggtgctg ctcacggaaa tcgacgcggc cgaactcgtg gcgccggtga agaaactgcg
     3361 cgacgagctc ttcgtcggga ttctcctcct cctgatcatc agcgtcgtca tcggccacca
     3421 ggtctcctac tccgtcgaca agccgctgca ggagctcggg gccttcgcgc accggatcgg
     3481 caccggggac ctgaccggac gcgcgaccgt gagcgggcac gacgtggccg gacggctggc
     3541 cgcctcgctc aacgagatgg ccgcgggcct ccaggagcgc gatcacgtga aggaagtctt
     3601 cggccggtac atcgccacgc aggtgtccga ccagattctc agcggccagg tgaacctcgg
     3661 cggcgaagcg cgcgtcgtca ccatcctctt ctcggacatc cgcaacttca ccggcatgtc
     3721 cgagcagatg acgccgcagc aagtcgtggc gttcctcaac gactattttt ccgagatggt
     3781 ggacgccgtc ttcgagcacc acggcgtgct ggacaagttc atgggcgacg gcctgatgg
//
LOCUS       DASTZR010000071         3515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1049224, whole genome shotgun sequence.
ACCESSION   DASTZR010000071 DASTZR010000000
VERSION     DASTZR010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049224"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VFV20_01580"
     CDS             <1..574
                     /locus_tag="VFV20_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287070.1"
                     /translation="ELAKRVRREADAYLATLEPIPAADLAQLAELLARGLAAILRSDV
                     PRDHIARTARFVGDAAIPMVALENAVFGLWQARDDCHMASWREGGFDGPTFDVLTHIW
                     RGAADSEDALATKVPQRPDDVRAAVARLRREGLVDPSRLAVTARGEKARQGIEDETDR
                     RFFEPWPDDVGARADWMRERLVAVNTALAP"
     gene            721..1413
                     /locus_tag="VFV20_01585"
     CDS             721..1413
                     /locus_tag="VFV20_01585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5287071.1"
                     /translation="MIVEDDPDIATLVANVLAEEGYETHIVTDSRQALDAFDRIRPNV
                     VTLDVMMPSFDGISLCLELRRTSDVPILFISARKEPPDRVVGLRIGADDYMGKPFDTE
                     ELVARVDALVRRSGVAQPGSEDRQIRFGRFVIDLGSVQAVAGEQQVQLTPTEFKLLRT
                     LAGEPGRVFTRDDLLTGVWGYEPGSDTRLVDVHVGRLRKKLDDAHVTEIQIETARGFG
                     YRLVDATRSARP"
     gene            complement(1374..2468)
                     /locus_tag="VFV20_01590"
     CDS             complement(1374..2468)
                     /locus_tag="VFV20_01590"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017616131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU5287072.1"
                     /translation="MTKTRDLRVDAYRPLIPPAVLLDELPLPEDGSRRIAGWREEVRR
                     ILAREDDRLVVVVGPCSVHDVAAAEDYARRLHDLSAALATELRIVMRVYFEKPRTTVG
                     WKGLINDPRLDGSFRINEGLRLARRLLLDVVALGLPAGCEFLDPISPQYTSDLVSWGA
                     IGARTTESQVHRELASGMSMPIGFKNGTGGTVQIAIDALRAAAYPHRFLGVTEQGLAA
                     IVSTRGNPDCHVILRGGARGPNYDAASIARALEQLEAAGLPGRLMVDASHGNSERDHR
                     RQPDVTRELGAQIASGERGIVGVMLESFLVAGRQDLGDPASLTYGQSITDACLGWDET
                     ETILRELAGAVRARRAAGQGRAERVASTSR"
     gene            2546..3073
                     /locus_tag="VFV20_01595"
     CDS             2546..3073
                     /locus_tag="VFV20_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEU5287073.1"
                     /translation="MPLYAYRGKRPRVAADAFIAPTAVLVGDVVVQSGASVWFGTVLR
                     GDMDRIEIGERSNVQDNSTIHTDENEPTLIGPDVTIGHMALVHSAVVERNVLIGQAAV
                     LVGRNRVGSGTIVGAGAVLPEGFDAPERSLVLGVPARVVRAVQEEDARWTVGAAAHYV
                     ALSTWYRENLEEVDA"
     gene            3087..>3515
                     /locus_tag="VFV20_01600"
     CDS             3087..>3515
                     /locus_tag="VFV20_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEU5287074.1"
                     /translation="MTTLRLTGHDLSIGDVVSVARGHVPVALDRVAERHVADAAKFVA
                     ELAAGNAPVYGVNTGFGDLSTVRIPTTDLRTLQRNLVRSHSVGVGEPLPEDVTRAILL
                     LRANTLAAGRSGVRVEVIELLLGMLDSGVHPVIPMHGSVGA"
BASE COUNT          506 a         1240 c         1245 g          524 t
ORIGIN      
        1 tgagctcgcg aagcgcgtgc gccgcgaggc ggacgcatac ctcgccacgc tcgagccgat
       61 ccccgcggcg gatctcgccc agctggcgga gctgctcgcg cgcgggctgg cggcgatcct
      121 tcgctccgac gtaccgcgcg accacatcgc gcggacggcg cgcttcgtcg gggacgccgc
      181 gatcccgatg gtcgccctcg agaacgccgt cttcggtctg tggcaggccc gcgatgactg
      241 tcacatggcc tcgtggcgcg agggcggctt cgacggcccc acgttcgacg tgctcacgca
      301 catctggcgc ggggcggccg actccgagga cgcgctcgcc acgaaggtgc cccagcggcc
      361 ggacgacgtg cgtgccgcgg tcgcgcgtct gcggcgcgag gggctcgtcg atccctcgcg
      421 gctcgcggtg accgcccgtg gcgagaaggc gcggcagggg atcgaggacg agaccgatcg
      481 gcgcttcttc gagccctggc ccgacgatgt cggcgcgcgc gccgactgga tgcgcgagcg
      541 cctggtcgcc gtcaacactg cgctcgctcc ctagccagcc gatctcgcgg gtatccatcg
      601 agattcatca cccatttgga acgttcccgt tacaaatggc acgggtcgtg cgacgggctt
      661 tccgtgatcg agccgcaggc cacgcagacc gggatgagga cccgtcatgg gacgaaggtg
      721 atgatcgtcg aggacgatcc cgatatcgcc acgctcgtcg ccaacgtgct cgccgaagag
      781 ggctacgaga cccacatcgt gacggactcg cggcaggctc tcgatgcgtt cgatcgcatc
      841 cgtccgaacg tcgtgacgct cgacgtgatg atgccgagct tcgatggcat cagcctctgc
      901 ctggagctgc ggcgcaccag cgacgtgccc atcctgttca tctccgcacg caaggagccg
      961 ccggatcgcg tcgtgggcct ccgcatcggc gcggacgact acatgggcaa gcccttcgat
     1021 acggaggagc tcgtcgcccg cgtcgacgcg ctcgtgcgcc ggtcgggagt cgcgcagccc
     1081 ggctccgaag atcgtcagat ccgcttcggc cggttcgtga tcgatctggg gagcgtgcag
     1141 gcggtggccg gcgagcagca ggtgcagctc acgccgaccg agttcaagct gctgcgcacg
     1201 ctcgcgggcg aaccgggccg cgtcttcacg cgggatgacc tgctcaccgg tgtctgggga
     1261 tacgagcccg gctccgacac gcggctcgtg gacgtgcacg ttgggcggct gcgcaagaag
     1321 ctcgacgacg cgcacgtgac ggagatccag atcgagacgg cgcgcgggtt cggctaccgg
     1381 ctcgtcgacg ccacgcgttc cgcgcgcccc tgacccgcgg cgcgacgcgc gcgcaccgca
     1441 ccggccagct cgcgcaggat cgtctcggtc tcgtcccacc cgaggcacgc atcggtgatg
     1501 ctctgcccgt acgtcagtga cgccggatcg ccgaggtcct ggcgtccggc gacgaggaag
     1561 ctctcgagca tcactccgac gatgccccgc tcgccggacg cgatctgggc gccgagctcg
     1621 cgcgtgacgt ccggctggcg acggtggtcg cgctcgctgt tgccgtggct cgcgtccacc
     1681 atgagccggc cgggcaggcc cgcggcctcg agctgctcga gcgcgcgcgc gatgctcgcg
     1741 gcgtcgtagt tcggtccgcg cgcgccgcca cggaggatca cgtggcaatc gggattgcca
     1801 cgggtgctga cgatcgccgc caggccctgt tcggtcacgc cgaggaagcg atgcggatac
     1861 gccgcggcac gcagggcatc gatggcgatc tgcaccgtgc cgccggtccc attcttgaaa
     1921 ccgatcggca tggacatgcc ggacgcgagc tcgcggtgca cctggctctc cgtcgtccgc
     1981 gcgccgatgg cgccccacga gacgaggtcg gaggtgtact gcggtgagat cgggtcgagg
     2041 aactcgcatc ccgccgggag gccgagcgcg acgacgtcga ggaggagacg gcgcgcgagg
     2101 cgcaggccct cgttgatccg gaatgacccg tcgaggcgcg ggtcgttgat gaggcccttc
     2161 cagccaactg tcgtccgcgg cttctcgaaa tacacgcgca tgacgatccg cagctccgtc
     2221 gcaagcgccg ccgacaggtc gtgcagcctg cgcgcgtagt cctcggcggc ggcgacgtcg
     2281 tgcacggagc acggtcccac gacgaccacg aggcggtcgt cctcccgggc caggatgcgc
     2341 cggacctcct cgcgccaccc ggcgatgcgc cgcgagccgt cctcaggaag cggcagctcg
     2401 tcgagcagga ccgcaggcgg gatgagcggc cggtaggcgt cgacgcggag gtcgcgggtc
     2461 ttggtcacgc tgccgccttc ggcgaccccg gacacaccga ccccacgggc ctcgctcccc
     2521 tcttcataca aatactctac gggcgatgcc gctttacgcc taccgtggaa agcgaccgcg
     2581 ggtcgccgcc gacgcgttca tcgcaccgac cgcggtcctc gtcggcgacg tcgttgtgca
     2641 gtctggtgcc tcggtctggt tcggcacggt gctccgcggt gacatggacc gcatcgagat
     2701 cggcgagcgc agcaacgtgc aggacaactc cacgatccac accgacgaga acgagccgac
     2761 gctcatcggt cccgacgtca cgatcgggca catggcgctc gtgcactcgg ccgtcgtcga
     2821 gcgcaacgtc ctgatcggtc aggccgcggt gctcgtcggg cgcaaccgcg tcggatcggg
     2881 gacgatcgtc ggcgcgggcg cggtgctgcc cgagggattc gacgcgccgg agcggtcgct
     2941 ggtgctcggc gttcccgcgc gcgtcgtgcg cgcggtgcag gaggaggacg cgcgctggac
     3001 ggtgggcgcc gcggcgcact acgtcgcgct ttcgacctgg tatcgcgaga acctcgaaga
     3061 ggtcgacgcg tgagagacgg gcgaggatga ccaccctgcg gctgaccggc cacgaccttt
     3121 ccatcggcga cgtcgtctcc gtcgcgcgcg gccacgtacc ggtcgcgctc gatcgcgtgg
     3181 cggagcgcca cgtcgccgac gccgcgaagt tcgtcgccga gctcgcggcc ggcaacgccc
     3241 ccgtgtacgg cgtgaatacc ggcttcggcg atctctcgac ggtgcggatc ccgacgaccg
     3301 acttgcgcac gctgcagcgc aatctcgtgc gcagccattc ggtgggcgtc ggtgagccgc
     3361 tgcccgagga cgtcacgcgc gcgatcctcc tgctgcgcgc gaacacgctc gccgcgggac
     3421 gttcgggtgt ccgcgtcgag gtcatcgagc tgctgctcgg catgctcgac agcggcgtcc
     3481 acccggtcat cccgatgcac ggcagcgtcg gcgcg
//
LOCUS       DASTZR010000072         4911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_892410, whole genome shotgun sequence.
ACCESSION   DASTZR010000072 DASTZR010000000
VERSION     DASTZR010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892410"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VFV20_01605"
     CDS             <1..407
                     /locus_tag="VFV20_01605"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU5287075.1"
                     /translation="TMIVQGHAQLATALISGERTVAIEADAGQIYPDIQKGTPIKILT
                     PTEGVFLINSPMAIPAKAPHPNAAKAFLEFNLGKEVQSLFTTDGTYSVRTDVAPPKTY
                     PELKSLKVVNVDYDAAEKDNKKTKDQFAEIFK"
     gene            437..2080
                     /locus_tag="VFV20_01610"
     CDS             437..2080
                     /locus_tag="VFV20_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU5287076.1"
                     /translation="MAASAGVLLALVGAPLAMLAWQSVSDKQGLTLDGYTAVVGRSLY
                     YQSLINTLEVGIGSAALSVLIGAPLAWFVARTDMPRRGLVRTLVTASYVAPPFLLAIA
                     YVILMAPNTGALNRAIVALTGVERGPFNAYSLGILIFVTGLHTFPAVFLLTVAALESL
                     DGSLEQAARILGAGKLRTTLKITLPLVLPSVLGGALLAFVTAIALFGSQAILGIPGRV
                     YTLPTRVYQVLGYPPDYTLASALSMLLVALTVIALALQRRWVGRRVATIGARTTPAEP
                     LGLGRWRWLALAASALVILVAIVLPYGALGAVSLMRAWPAGLVAANLTLDNYAQVLFG
                     LEVTRRAIGNSLLLGALTATITMVLGVVIATIDLRTALRGKRVLDYLSVIPLGVPGIV
                     LAVAILELWLRLPVNLYGTLAILLLAYLTRFVPLAVRSSQAAISQVDTSLEEAARIGG
                     ASWWTTERRVTLPLAAPALVAGWILVFVPTLQELSASVLLFTPDTMTLAVTIFNFQDN
                     GKLELVSALGVVMLVLVTFLVALTRRFAGRPVVPSVRGV"
     gene            2083..3174
                     /locus_tag="VFV20_01615"
     CDS             2083..3174
                     /locus_tag="VFV20_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5287077.1"
                     /translation="MGSLRLENVSLRYPGRDLAAVTDVSLEIRDGEFLVLVGPSGCGK
                     STTLRLIAGFLVPDAGRILADDVVLSQPGRALPPERRNMAMVFQSFAVWPHLTVFENV
                     AFGLRARRIAGAEIRRRVDAMLAMVDLGGFGPSYPSQLSGGQQQRVALARALVVEPRT
                     LLLDEPLSSLDARLRERMCGELKDLQRRTGVTFVHVTHDQSEAVALGDRVVVMHAGRI
                     QQIGTPSDVYLRPANREVVEAMGAANVLAGHVVAVSAGTASVRLLSDPSLVLAVPCEH
                     QFVAGAPVVLAVRPEDVSLADEGAPGVPGTVESATFRGALADHLVAVAGEGPAAPLRL
                     RANTLGRAARGAGSRVSVQIDGTRCALLR"
     gene            3601..4002
                     /locus_tag="VFV20_01620"
     CDS             3601..4002
                     /locus_tag="VFV20_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015353635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEU5287078.1"
                     /translation="MQQRDTYASAGGELNALLGRGSEFEGKLAFEGKVRIDGTFTGEI
                     STNDLLQVGDGAKVQAEISCGTIIVEGEITGNIKATQAVELRRPAKVHGDITTPSLVI
                     EKGVLFEGRSRMDEVSSSSNVVPIYAAADDK"
     gene            4029..4748
                     /locus_tag="VFV20_01625"
     CDS             4029..4748
                     /locus_tag="VFV20_01625"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEU5287079.1"
                     /translation="MQGVLMLSRVTVRDADFRRVTFDQFAPEGCTFEHCDFSGQTLDR
                     RYQPLFSSRRQSIFRDCSFDGADLTGVRPGQARFERCSFADAKIDAWESFTAEFVDCV
                     FAGRVHNVRFYGRPWGAHAEQLDPARKVNAFTGNDFRKADLMGVTFIHGVDIGKQRWP
                     VGPQYVILDRIHQRIAKARVGVLDWHDHKARQEALDMLQAASLLYAHQMTVIGRRVEE
                     RWTAQPAVQERVWDALTRAIA"
BASE COUNT          674 a         1757 c         1694 g          786 t
ORIGIN      
        1 ggacgatgat cgtgcagggc cacgcgcagc tcgcgacggc cctcatctcc ggcgagcgca
       61 ccgtcgcgat cgaggccgat gccggccaga tctacccgga catccagaag ggcacgccga
      121 tcaagatcct gacgccgacc gaaggggtgt tcctcatcaa ctcgcccatg gcgatcccgg
      181 caaaggcgcc gcacccgaac gccgcgaagg ccttcctcga gttcaacctc ggcaaggagg
      241 tccagtcgct cttcacgacg gacggcacgt actcggtgcg caccgacgtc gcaccgccga
      301 agacgtaccc cgagctgaag tcgctcaagg tcgtgaacgt cgactacgac gcggccgaga
      361 aggacaacaa gaagaccaag gaccagttcg cggagatctt caagtaggtc gcagatcgct
      421 ggccggcgtg gccgtgatgg ccgcgtcggc cggcgtgctc ctcgcgctcg tcggcgcgcc
      481 gctcgcgatg ctcgcctggc agagcgtcag cgacaagcag ggtctcaccc tcgacggcta
      541 taccgccgtc gtcggacgca gcctgtatta ccagtcgctc atcaacacgc tcgaggtcgg
      601 cataggctcg gccgcgcttt ccgtcctcat cggcgccccg ctcgcatggt tcgtcgcgcg
      661 gaccgacatg ccgcggcgcg gtctcgtgcg cacgctggtg accgcgtcgt acgtcgcgcc
      721 gccgttcctg ctcgcgatcg cgtacgtcat cctcatggcg cccaataccg gcgcgctcaa
      781 ccgcgcgatc gtcgcgctga cgggcgtcga gcgcggcccg ttcaacgcgt actcgctcgg
      841 gatcctcatc ttcgtcaccg gcctccacac gttccccgcg gtgttcctgc tcacggtcgc
      901 ggcgctggag tccctcgacg ggtcgctcga gcaggccgcg cgcatcctcg gggcggggaa
      961 gctgcgcacg acgctcaaga tcacgctccc gctggtcctg ccgagcgtcc tcggcggggc
     1021 gctgctcgcc ttcgtcaccg cgatcgccct gttcggctcc caggccatcc tgggcatccc
     1081 cgggcgcgtc tacacgctcc cgacgcgcgt gtatcaggtg ctgggctacc cgccggacta
     1141 cacgctggcg agcgcgctca gcatgctgct ggtcgccctg acggtgatcg cgctggccct
     1201 ccagcggcgg tgggtggggc gccgtgtcgc gacgatcggc gcgcggacga cgccggcgga
     1261 gccgctcggg ctcggcaggt ggcgctggct cgcgctcgcg gcctcggcgc tcgtcatcct
     1321 cgtggcgatc gtgctgccct acggcgcgct cggcgcggtg tcgctcatgc gtgcgtggcc
     1381 cgcgggcctc gtggccgcga atctcacgct cgacaactac gcgcaggtgc tcttcggcct
     1441 cgaggtcacc cgtcgcgcga tcggcaacag cctgctcctc ggggcgctca cggcgacgat
     1501 cacgatggtg ctcggcgtcg tcatcgccac gatcgacctg cggaccgcgc tgcgcggaaa
     1561 gcgcgtcctc gactacctca gcgtgatccc gctgggggtg ccagggatcg tgctcgcggt
     1621 cgcgatcctc gagctgtggc tgcggctgcc ggtcaacctc tacggcacgc tcgcgatcct
     1681 cctgctcgcg tacctcacgc ggttcgtgcc gctcgcggtg cgttcctcgc aggcggccat
     1741 cagccaggtc gatacgtcgc tcgaagaggc cgcgcgcatc ggcggcgcga gctggtggac
     1801 gacggagcgg cgcgtcacgc tcccgctcgc cgcgcccgcg ctcgtcgcgg gatggatcct
     1861 cgtcttcgtg ccgacgctcc aggagctctc cgcgtccgtg ctcctgttca cgccggacac
     1921 catgacgctg gccgtcacca tcttcaattt ccaggacaac gggaagctcg agctcgtgag
     1981 cgcgctcggt gtcgtcatgc tggtgctcgt gaccttcctc gtcgccctca cgcggcgctt
     2041 cgcgggacgg ccggtggtgc cgtcggtacg gggcgtctga ccatgggatc gctccggctg
     2101 gagaacgtga gcctgcgcta tccgggacgc gacctcgctg cggtgaccga cgtgtcgctg
     2161 gagatccgcg acggcgagtt cctcgtgctc gtcgggccgt cgggctgcgg gaagagcacc
     2221 acgctccggc tcatcgcggg attcctcgtg cccgacgccg gccgcatcct cgcggacgac
     2281 gtggtgctgt cgcagccggg ccgcgcgctc ccgcccgagc ggcgcaacat ggccatggtc
     2341 ttccagagct tcgcggtctg gccgcatctc acggtcttcg agaacgtcgc cttcggactg
     2401 cgcgcgcgcc gtatcgcggg cgctgagatc cgcaggcgcg tcgacgcgat gctcgcgatg
     2461 gtcgacctcg gcggcttcgg gccgtcctat ccatcgcagc tctccggcgg tcagcagcag
     2521 cgcgtggcgc tcgcgcgcgc gctcgtggtc gagccgcgca cgctgcttct cgacgaaccg
     2581 ctgtccagcc tcgacgcccg gctgcgcgag cggatgtgcg gcgagctcaa ggacctgcag
     2641 cgccgcaccg gcgtcacgtt cgtccacgtg acccacgatc agtccgaggc cgtcgccctc
     2701 ggcgatcgcg tcgtcgtcat gcacgccggc cgcatccagc agatcgggac gcccagcgac
     2761 gtgtatctcc gtccggccaa ccgcgaggtc gtcgaggcca tgggcgcggc gaacgtcctc
     2821 gccggccacg tcgtcgcggt gtccgcgggc accgcctcgg tgcgccttct ctccgatccg
     2881 tcgctcgtcc tggcggtgcc ctgcgagcac cagttcgtgg ccggcgcgcc cgtcgtgctc
     2941 gccgtgcggc ccgaggacgt ctcgctcgcg gacgagggcg cgccaggggt ccccggcacg
     3001 gtcgagagcg cgaccttccg gggtgcgctc gcggaccacc tggtggcggt cgcgggtgag
     3061 gggccggcgg caccgctgcg gctgcgggcc aacaccctgg gccgggcggc ccgcggcgcc
     3121 ggcagccgcg tctccgtcca gatcgacggc acccgctgcg ccctgctgcg ctgagccgac
     3181 gggtgccgcc cgcttgcccg ggcgtcaggc aggggcggac aatcgccgac ggtggggatg
     3241 cggctgggga ccgtcgttgc ggtcgggttc gtctttgggg cgtcgctggg gttcctggcg
     3301 ggcctgttcc tggccatccc ggccgccgct gtcgtgcccg gcggccgacc cgtgatcttc
     3361 ggagctgccg gcctcggtgc gctcgtcggt tcggccctcg cggcgtggcg ttcgtcgggc
     3421 ggattgccac gcgaacgttc tggccgattc cgcgttgagg aagtaatgtc ccgacttacg
     3481 ttcaagcgac cgagtgacag atcgcgtccc gacaagggga gctcgtcgac gacgccggtg
     3541 tggcgtcccg ccgaaccgcc tacgcctggg cgcagtgagg aaccagcgga gggaacggca
     3601 atgcagcagc gagacaccta cgcgtcggcg ggcggcgagc tcaatgccct gctcgggcga
     3661 ggctccgagt tcgagggcaa gctcgcattc gagggcaagg tccgcatcga cgggacgttc
     3721 acgggcgaga tctcgacgaa cgacctgctc caggtcggcg acggcgccaa ggtccaggcc
     3781 gagatcagct gcgggacgat catcgtcgag ggcgagatca cggggaacat caaggccacg
     3841 caggccgtcg agctccgccg gccggcgaag gtccacggcg acatcacgac gccgtcgctg
     3901 gtgatcgaga agggcgtgct cttcgaaggt cgctcgcgga tggacgaggt ctcgtcgtcg
     3961 tcgaacgtcg tcccgatcta cgccgccgca gacgacaagt agcccgaccg cagtagagcc
     4021 gctcttcgat gcagggcgtc ctgatgctca gtcgtgtcac cgtccgcgac gcggacttcc
     4081 gccgcgtcac gttcgatcag ttcgcccctg agggctgcac cttcgagcac tgcgacttct
     4141 ccggtcagac gctcgaccgt cgctaccagc cgctcttctc cagccggcgt cagtcgatct
     4201 tccgcgactg cagcttcgac ggcgccgacc tcacgggagt gcggccggga caggcgcgct
     4261 tcgagcgctg ctcgttcgcc gatgccaaga tcgacgcctg ggagtcgttc accgcggagt
     4321 tcgtggactg tgtgttcgcg ggccgcgtgc acaacgtgcg cttctacgga cgcccgtggg
     4381 gcgcgcacgc cgagcagctc gaccccgcgc gcaaggtcaa cgcgttcacc ggcaacgact
     4441 tccgcaaggc cgacctgatg ggcgtgacct tcatccacgg ggtggacatc gggaagcagc
     4501 gctggcctgt gggaccgcag tacgtgatcc tcgatcgcat ccatcagcgg atcgcgaagg
     4561 cacgcgtcgg cgtgctcgac tggcatgacc acaaggctcg ccaggaggcg ctcgacatgc
     4621 tgcaggcggc gtcgctcctg tacgcgcacc agatgacggt catcggccgc cgcgtcgagg
     4681 agcgctggac cgcccagccc gccgtgcagg agcgcgtctg ggacgcgctc acccgggcca
     4741 tcgcctagca gatccgcggc gtccgctcgg acgtcgcgag agatgagcga cgtcctcgat
     4801 gcgctcctcc gatccaccga accgtcgatc cgctggaagg cgcgtgggga agaacgctag
     4861 actgatcgga cgcgatgacg cggacagcga tcgcgggtag cgcgaaagcg a
//
LOCUS       DASTZR010000073         4393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_735862, whole genome shotgun sequence.
ACCESSION   DASTZR010000073 DASTZR010000000
VERSION     DASTZR010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735862"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VFV20_01630"
     CDS             complement(<1..192)
                     /locus_tag="VFV20_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287080.1"
                     /translation="MDRDDRRTRTGALVGALAMFLALDVAFLASLIGLPFTPFALGQA
                     IIDVLPGAISIPLIETLQYW"
     gene            213..2216
                     /locus_tag="VFV20_01635"
     CDS             213..2216
                     /locus_tag="VFV20_01635"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU5287081.1"
                     /translation="MAELPPPMTPEDLYAFRFVADAQLSPDGERVAYVVREIDREKDS
                     YRSAIWLIPFGGGSAARFTSGPGQDAQPRWSPDGRTLAFVSDRNAPAGDPKARKPKNI
                     FVLSLDGGEARQLTTFDDDCGDLTWSPDGTRIAFTLKEPKPKDAADDGGIRVYERMRY
                     KTDEGYLLDGRRRHVWTADVAGGGVRKLTDSDWDDVQPAWSADGTKIAFVSNRTDDRE
                     KNTVADIHVATLQGATWRVSDGVGSYSSPRFSPDGTQIACFGVAKAVGSSAKNVRLFL
                     FDAGSADQQGGPGTDLTGDWDRTVGSSVMSDMRGQSYTLPPTWTADRARIVFVGSDRG
                     TANVYSASVKGGDVRAETTGAHQVVSLSLAAGAKRFACVFSHATLPGDVAVGELGGSP
                     KPITDVNGELLRSRYIVSPEHVEFSGADGWKIDGWVLKPRGFDAAKKWPMVLEVHGGP
                     HGAYGHGFFHEFQVLAGRGYGVLFTNPRGSQAYGEKFSEACVGDWGGKDYEDLMAGVD
                     HALTLGWVDPKRLYVTGGSYGGFMTNWIVGHTDRFRAAATQRSISNNVSAFGVSDIGW
                     HFWEYEMGDASPWKDVAKLVERSPLYHAPKVKTPLLILHAERDVRCPIEQAEQFFIAL
                     RYHGVETRFVRFPEDNHDLTRGGKPRNRVEHCRRIADWFDAHP"
     gene            complement(2217..2828)
                     /locus_tag="VFV20_01640"
     CDS             complement(2217..2828)
                     /locus_tag="VFV20_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEU5287082.1"
                     /translation="MLGDWATFFLALLLVPVLVLEESSADPGTVQLATLANAFIWAAF
                     ALDYALDVRRAADRHEYVRTHWFDLALILVSPPLLVPAEAQALRVLRALRSLRAFVVV
                     GIVAERLGRPLSRQAALALLAVLGTVLLAGGVMITAAEPAIIANVAQGYAWTIATVVT
                     AGHASPQPTTLMGRAISSAIVVMGLGTLAALAASVAGTRPKVP"
     gene            2856..3383
                     /locus_tag="VFV20_01645"
     CDS             2856..3383
                     /locus_tag="VFV20_01645"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5287083.1"
                     /translation="MSVRIATPDDADAIERVRTDTWRAAYHGLLPARVLDRLGYDGTR
                     RRQTMEMMPRDRFVLVAEHDGEIVGFVNGGPSRVADASHTGEIYAIYVLPSHQSHGHG
                     SALLRAGARELLARGSRGMLIWVLRENLPSRRFYERMGGRHLRDRDEDREIEGVIVTE
                     AGYAWDDVTPLATRA"
     gene            complement(3370..3795)
                     /locus_tag="VFV20_01650"
     CDS             complement(3370..3795)
                     /locus_tag="VFV20_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287084.1"
                     /translation="MAEDARRVRTPHGELTLEEIAELLPGTGELMQSVGNTWWKCAYA
                     ARGGNWELAAYFARRTRGLLHKLAIVRPKYAGDIASFEAEHIAAVLAACERRASQDTE
                     AAVAAAIDAANELHAKWGKSYIRWTLPDAPPTDLELTRE"
     gene            3816..3974
                     /locus_tag="VFV20_01655"
     CDS             3816..3974
                     /locus_tag="VFV20_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287085.1"
                     /translation="MRRRRGSPLRAIRNFTDSQIPLPRRTAVAMRNLWLRVALRQNCC
                     GHDGEPGC"
     gene            4032..>4393
                     /locus_tag="VFV20_01660"
     CDS             4032..>4393
                     /locus_tag="VFV20_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287086.1"
                     /translation="MKEFLEGRRLGFAPRSHLAFLACGLALGVTIADVMAWLALGSSE
                     TNGFVIVAQWLAVTTLLVAAAAAIAAVAERWDVIEEERGLARIDILAALGVVALYGIS
                     VVLRSFELGNPAPAPAPFL"
BASE COUNT          665 a         1544 c         1562 g          622 t
ORIGIN      
        1 ccagtactgg agcgtctcga tcagcgggat gctgatcgcg ccgggaagca cgtcgatgat
       61 cgcctggccg agggcgaatg gcgtgaacgg caggccgatg agcgacgcga ggaaggccac
      121 gtcgagggcg aggaacatcg cgagcgcgcc gacgagtgca cccgtgcgcg tccgccggtc
      181 gtcccggtcc acctatggca cgatacgacc ccttggccga gcttccgcct ccgatgacgc
      241 ccgaggacct ctacgcgttc cgcttcgttg ccgatgcgca gctctcgccg gatggcgaac
      301 gcgtcgcata cgtggtgcgc gagatcgatc gcgagaagga ctcgtatcgc tcggcgatct
      361 ggctgatccc gttcggcggc gggagtgccg cgcgattcac cagcggtccc ggtcaggatg
      421 cacagccgcg ctggtcgccc gacggccgca cgctcgcgtt cgtgtcggac cggaacgcac
      481 ccgcgggcga cccgaaggcg cggaagccga agaacatctt cgtgctgtcg ctcgacggag
      541 gcgaggcgcg ccagctcacg acgttcgacg acgactgcgg cgacctcacc tggtcgccgg
      601 acgggacccg catcgcgttc acgctgaagg agccgaagcc gaaggacgcg gcggacgacg
      661 gcggcatccg cgtgtacgag cgcatgcgct acaagaccga cgagggctac ctcctcgacg
      721 gccggcggcg tcacgtatgg accgcggacg tcgcgggtgg cggggtgcga aagctcaccg
      781 atagtgattg ggacgacgtc cagccggcgt ggtccgccga cgggacgaag atcgcgttcg
      841 tctcgaaccg caccgacgac cgcgagaaga acaccgtcgc cgacatccac gtggcgacgc
      901 tgcagggcgc gacctggcgc gtgagcgacg gcgtcgggtc gtacagcagc ccgcggttct
      961 cgccggacgg cacgcagatc gcgtgcttcg gtgtcgcgaa agcggtcggc tcgtcggcga
     1021 agaacgtgcg cctcttcctc ttcgacgcgg gctccgcgga ccagcagggc ggacccggca
     1081 ccgacctcac cggcgactgg gaccgcaccg tggggtcgtc ggtcatgtcg gacatgcgcg
     1141 ggcagtcgta cacgctgccg ccgacgtgga ccgccgaccg cgcgcggatc gtcttcgtcg
     1201 gctcggaccg aggcaccgcg aacgtctaca gcgcctcggt gaagggcggc gacgttcgcg
     1261 ccgagaccac cggcgcgcat caggtcgtga gcctgtcgct cgccgcgggc gcgaagcgct
     1321 tcgcgtgcgt gttctcgcat gcgaccctgc cgggtgatgt cgcggtcggc gagctcggcg
     1381 ggtcgccgaa gccgatcacc gacgtgaacg gcgagctgct gcgctcgcgc tacatcgtct
     1441 cgccggagca cgtcgagttc agcggcgccg acggctggaa gatcgacggc tgggtgctga
     1501 agccgcgcgg gttcgacgcc gcgaagaagt ggccgatggt gctcgaggtg catggcggcc
     1561 cgcacggcgc gtacggccac gggttcttcc acgagttcca ggtgctcgcc ggccgcggct
     1621 acggagtgct gttcaccaac ccgcgcggca gccaggcgta cggcgagaag ttctcggagg
     1681 cgtgcgtcgg cgactggggc gggaaggatt acgaggacct catggccggc gtcgatcacg
     1741 cgctcacgct cggctgggtg gacccgaagc gcctctacgt gaccggcggc agctacggcg
     1801 ggttcatgac gaactggatc gtcggccaca ccgaccgctt ccgcgccgcc gcgacgcagc
     1861 ggagcatcag caacaacgtc tccgccttcg gcgtctcgga catcggctgg cacttctggg
     1921 agtacgagat gggcgacgca tcaccgtgga aggacgtcgc gaagctcgtc gagcgctcgc
     1981 cgctgtacca cgcgccgaag gtgaagacgc cgctcctcat cctgcacgcc gagcgcgacg
     2041 tgcgctgtcc catcgagcag gccgagcagt tcttcatcgc gctccgctac cacggcgtcg
     2101 agacgcggtt cgtccgcttt cccgaggata accacgacct gacccgcggc ggcaagccgc
     2161 ggaaccgcgt ggagcattgc cggcggatcg cggactggtt cgacgcccac ccgtagtcag
     2221 gggactttcg gccgcgttcc tgcgaccgac gcggcgagcg ccgcgagcgt gccgaggccc
     2281 atcaccacga tcgccgatga gatcgcgcga cccatgagcg tcgtcggctg cggcgaggcg
     2341 tgcccggcgg tcacgacggt cgcgatcgtc cacgcgtagc cctgcgcgac gttcgcgatg
     2401 atcgccggct ccgcggccgt gatcatcacg ccgcccgcga ggaggaccgt gccgaggacc
     2461 gcgaggagcg cgagcgcggc ctgccgcgac agcggacgtc cgagacgctc ggccacgatc
     2521 ccgaccacga cgaacgcgcg cagcgaccgc agcgcacgga gcacgcgaag cgcctgcgcc
     2581 tccgcgggca cgaggagcgg gggagagacg aggatgagcg cgaggtcgaa ccagtgtgtc
     2641 cgcacgtact cgtgccggtc ggccgcccgt cggacgtcca gcgcgtagtc gagcgcgaac
     2701 gccgcccaga tgaacgcgtt cgcgagcgtg gcgagctgga cggtcccggg gtccgcgctc
     2761 gactcctcga ggaccagcac cggcacgagg agcagcgcga ggaagaacgt cgcccagtcg
     2821 ccgagcatgg cgagcgcaag cctaggattc gcggcgtgag cgtgcggatc gcgacgccgg
     2881 acgacgccga tgcgatcgag cgcgtccgga ccgacacgtg gcgcgctgcg taccacgggc
     2941 tcctgccggc gcgggtcctt gaccggctcg gctacgacgg cacgcgacgg cgacagacca
     3001 tggagatgat gccgcgcgat cggttcgtgc tcgtggctga gcacgacggc gagatcgtcg
     3061 gcttcgtgaa cggcggaccc tcgcgcgtcg ccgacgcatc gcacaccggc gagatctacg
     3121 cgatctacgt gctgccgtcg caccagtcac acggccacgg gagcgcgctg ctgcgtgccg
     3181 gtgcccgcga gctcctcgcg cgcggatcgc gcggcatgct catctgggtc ctgcgcgaaa
     3241 acctgccctc acgccgtttc tacgagcgca tgggcggacg ccacctgcgt gatcgtgacg
     3301 aggatcgcga gatcgagggc gtgatcgtca ccgaggcggg gtacgcctgg gacgacgtca
     3361 caccgctcgc tactcgcgcg tgagctccag gtccgtcggc ggcgcgtcag gcagcgtcca
     3421 gcggatgtac gacttgcccc acttcgcgtg cagctcgttc gccgcatcga tggcggccgc
     3481 gacggcggcc tccgtgtcct gcgacgcacg gcgctcgcag gcggccagga ccgcggcgat
     3541 gtgctcggcc tcgaagctcg cgatgtcgcc cgcgtacttc ggccgaacga tcgcgagctt
     3601 gtgaaggagc ccgcgcgtgc gccgcgcgaa gtacgccgcg agctcccagt tgccgccgcg
     3661 ggcggcgtag gcgcacttcc accaggtgtt cccgaccgac tgcatcagct caccggtgcc
     3721 cgggagcagc tccgcgatct cctcgagcgt gagctccccg tgcggggtgc gtacacgcct
     3781 tgcgtcctcg gccacgcggc cgatagtacg tagccatgcg ccgccgccgg ggatcgccgc
     3841 tccgggccat ccggaacttc accgattccc agatcccgct gccccgccgc acggccgtgg
     3901 cgatgcgcaa cctgtggctc cgggtcgccc tgcgccagaa ctgctgcggg cacgacggcg
     3961 agcccggctg ctgactgccc gcgccgacgg agggtcctcc gtcccgccag accggctagc
     4021 atgagcctcc cgtgaaggaa ttcctcgaag gacggcgtct gggcttcgca ccccgctcgc
     4081 atctcgcgtt cctggcgtgc gggctcgcgc tcggcgtcac gatcgctgac gtcatggcgt
     4141 ggctcgcgct cggctcgtcc gagacgaacg gcttcgtcat cgtcgcgcag tggcttgccg
     4201 tcacgacgct gctcgtggcg gccgcggccg cgatcgccgc ggtcgcggag cgctgggacg
     4261 tgatcgaaga ggagcgtgga ctcgcgcgca tcgacatcct cgcggcactc ggcgtcgtcg
     4321 ccctctacgg catctcggtc gtgctccgca gcttcgagct cgggaacccc gcccccgcgc
     4381 ccgctccgtt cct
//
LOCUS       DASTZR010000074         2039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1204774, whole genome shotgun sequence.
ACCESSION   DASTZR010000074 DASTZR010000000
VERSION     DASTZR010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204774"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VFV20_01665"
     CDS             <1..292
                     /locus_tag="VFV20_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEU5287087.1"
                     /translation="SDILEGITRAGLIDLARRELGLETVIRPIDRSELYVADEVFFCG
                     TGAQVAPCVSIDHRTVGDGKIGPITRRVGDLYYAVAHGDDDAYPEWRTAVYR"
     gene            289..1152
                     /gene="pheA"
                     /locus_tag="VFV20_01670"
     CDS             289..1152
                     /gene="pheA"
                     /locus_tag="VFV20_01670"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU5287088.1"
                     /translation="MSVAYQGLAGAFSEAAAAALFPDATPVAKRTFADVFAALEGAEV
                     DAAVVPVENSHAGSVADVYDLLRTHRNARVVAEAMVRVRHSLLGIPGATLDRIRLARS
                     HPQALAQCEDFLRKLNIQPQVAYDTAGAAMEVAQVGNPAVAAIASRRAAKQYGLTVLA
                     DGIETSSDNVTRFFALAREGDDAVARRVAHEHAAGPLKTSLVYTTKNEPGALTRSLQP
                     FATAGLQLTKIESRPSRAATWDYVFYLDFQGDPCVTPGREALALMRTCCEWVQVLGTY
                     PAATTVLEPDR"
     gene            complement(1149..>2039)
                     /locus_tag="VFV20_01675"
     CDS             complement(1149..>2039)
                     /locus_tag="VFV20_01675"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5287089.1"
                     /translation="MLGGGVLLVVLFAIVEIRRERAGRFFLFDFDLWRYPAFRYGNLA
                     GTIVSLGEFGLLFVLPLFLQAVVGYTAFETGLTFLALALGSFVSAGGASVLTRRLGAR
                     RVVTIGMALEAIGILGATLLITVSVNGLMLAAPLFVYGIGVGFATAQLTNIVLSDVPG
                     ERSGLASGTNSTMRQLGSALGIAILGTVLFTSLADGTRDRLTAMGVPPAARDAVVTVM
                     DSSAGQALPGLRQDPRGAAAIPAIESAFAEAARSAGFVAFGFVLVGLALSLLIPTPQR
                     VFTGRRVEGEARADPARSAS"
BASE COUNT          334 a          726 c          726 g          253 t
ORIGIN      
        1 ctccgacatc ctcgagggca tcacgcgcgc cgggctcatc gacctcgcgc gccgcgagct
       61 cggcctcgag acggtcatcc gaccgatcga ccggagcgag ctctacgtcg ccgacgaggt
      121 gttcttttgc gggaccggcg cgcaggtcgc gccctgcgtc tcgatcgatc accgcaccgt
      181 cggcgacggc aagatcgggc cgatcacgcg gcgcgtgggc gacctgtact acgccgtcgc
      241 gcatggtgac gacgacgcgt atccagagtg gcgcacggcg gtctaccgat gagcgtcgcc
      301 taccaggggc tcgccggcgc gttctcggaa gccgcggccg cggcgctctt cccggatgcg
      361 acgcccgtcg cgaagcgcac cttcgccgac gtgttcgcgg cgctcgaggg tgcggaggtc
      421 gacgccgccg tggtgcccgt ggagaactcg cacgccggtt cggtcgccga cgtgtacgac
      481 ctcctgcgca cccaccggaa cgcgagggtc gtggcggagg cgatggtccg cgtgcgtcac
      541 tcgctgctcg ggatccccgg cgcgacgctc gaccgcatcc ggctcgcacg ctcgcatccg
      601 caggccctcg cgcagtgcga ggacttcctg cgcaagctca acatccagcc gcaggtcgcg
      661 tacgacacgg cgggcgcggc gatggaggtc gcgcaggtcg ggaatccggc ggtcgcggcg
      721 atcgcgagcc gccgcgccgc gaagcagtac gggctcaccg tcctcgcgga cggcatcgag
      781 acgagcagcg acaacgtcac gcgcttcttc gcgctggcgc gtgagggcga cgatgccgtc
      841 gctcgccgcg tcgcgcacga gcacgccgcg gggccgctga agacgagcct cgtgtacacg
      901 acgaagaacg agcccggtgc gctcacgcgt tcgctccagc ctttcgccac cgcgggactg
      961 cagctcacga agatcgagtc gcggcccagc cgcgcggcga cgtgggacta cgtcttctac
     1021 ctcgacttcc agggcgatcc ctgcgtcacg cccggacgcg aggcgctcgc gctcatgcgg
     1081 acctgctgcg agtgggtgca ggtgctcggc acgtatccag ctgcgacgac cgtgctcgaa
     1141 ccggaccgct aggacgcgct ccgcgccggg tcggcgcgcg cttctccttc gacgcgacgt
     1201 ccggtgaaga cgcgttgcgg ggtggggatg agcagcgaca gcgccaggcc gaccagcacg
     1261 aagccgaacg cgacgaagcc agccgagcgc gccgcctcgg cgaacgcgct ctcgatggcg
     1321 gggatcgccg ccgcgccccg cggatcctgc cgcaggccgg gcagcgcctg gcccgcggag
     1381 ctatccatga ccgtgaccac cgcatcgcgc gccgccggcg ggacgcccat ggccgtgaga
     1441 cgatcgcgcg tcccatccgc gagcgaggtg aagagcaccg tcccgaggat ggcgatgccg
     1501 agcgccgaac cgagctgtcg catcgtcgag ttcgtgccgg acgcgagccc ggatcgctca
     1561 ccggggacgt ccgagaggac gatgttcgtc agctgcgccg tggcgaaccc cacgccgatg
     1621 ccgtacacga agagcggcgc ggcgagcatg aggccgttca ccgagaccgt gatgaggagc
     1681 gtggcgccga ggatgccgat cgcctcgagc gccatgccga tcgtcacgac acgacgtgcg
     1741 ccgagccgac gcgtgagcac gctcgcaccg ccggcggaga cgaacgaccc cagcgcgagc
     1801 gcgaggaacg tgagaccggt ctcgaacgcg gtgtatccga cgaccgcctg gaggaagagc
     1861 gggagcacga acagcagacc gaactcgccg agcgagacga tcgtgcccgc gagattcccg
     1921 tagcggaagg ccgggtagcg ccacaggtcg aagtcgaaga ggaagaagcg tccggcgcgc
     1981 tcgcggcgga tctccacgat cgcgaagagc accaccagga gcacgccacc gccgagcat
//
LOCUS       DASTZR010000075         5347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_735974, whole genome shotgun sequence.
ACCESSION   DASTZR010000075 DASTZR010000000
VERSION     DASTZR010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735974"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VFV20_01680"
     CDS             complement(<1..356)
                     /locus_tag="VFV20_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287090.1"
                     /translation="MPQPLSEFALKNKRTGQRSTKCRSCQRAYAKEHYRRNRGAYLDK
                     AGRRNARERDRFARFLLEYFRDHPCVDCGATDPRLLEFDHRDGVEKVAAVASVVRAQQ
                     WDAMVAEIAKCDVRCA"
     gene            988..1812
                     /locus_tag="VFV20_01685"
     CDS             988..1812
                     /locus_tag="VFV20_01685"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEU5287091.1"
                     /translation="MKFEGIEVQRAMVVYAHPDDAEFGLAGTIAKWARAGVEITYCMV
                     TNGASGSDDPDITRDQLRDIRYAEQREAAKVLGVKHCVFLGYEDGYLYPTLEVRRDVA
                     RQIRIHRPDVLITMDPTMRIAEDFYVNHPDHIAAGEVALRSINPDASTRQMFPELWRD
                     EHLEPHKPALLMLQSFGAGGTVVDISSTLETKVKALLLHRSQLWPGAEELVRDFASRI
                     GKAGGYKAGESFRVVRLERPEEPPKASRGKRGVQRHRPTSTRSRTKGTPSRRKRSP"
     gene            complement(1845..2180)
                     /locus_tag="VFV20_01690"
                     /pseudo
     CDS             complement(1845..2180)
                     /locus_tag="VFV20_01690"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
     gene            2079..3158
                     /locus_tag="VFV20_01695"
     CDS             2079..3158
                     /locus_tag="VFV20_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEU5287092.1"
                     /translation="MALPAPELLDAAKDAGLRYATDDRPGIRRHRAGHGFRYVGADGE
                     PIDDEKELARIRRLAIPPAWTDVWICPDARGHIQATGRDAKGRKQYRYHERFREIRDE
                     AKYERVLDFARALPKIRERVDADLRSPKLTRERVIATIVRLLEITLIRVGNEEYARAN
                     KSYGLTTLREKHVEVDGSRIRFRFRGKSGKEHDVELSDRRVARVLRALQELPGQELFR
                     YVGDDGDVRTVSSEDVNTYLREIVGADFTAKDFRTWAGTVLAAVALGAQDHIENQRMA
                     KKNVTTAIEQVAARLGNTRTVCRKCYVHPAVVDAYLDGELARAMQGRAEGEPADDNAL
                     TADEKLVLAFLERRLAKAARPASAA"
     gene            complement(3183..5087)
                     /locus_tag="VFV20_01700"
     CDS             complement(3183..5087)
                     /locus_tag="VFV20_01700"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU5287093.1"
                     /translation="MERVLDKRYKIVSPIGAGGSSQVYLAQDTALNREVAIKVLDAAA
                     AADPTLRKMFVKEARALASLSHPSIVSVFDVGEVDGVPFIVMEHLPGGSLKQRIERSG
                     PLAVGEAAAHTIEIANGLAFAHSRGIIHADLKPSNILFDANDHAKICDFGIARTPQEE
                     ADTPQLFATAMYVAPERVEGKPATQTSDVYGLGLLLYEMLVGRPPFVSANAAVLLRDH
                     VVRMPVPPSHLRQSLVRELDTIVLKALAKEPRLRYQKASELATALSSIENLDRELATT
                     RIVGVMTEPIRDFVPTVTQSPVVALLSEYGQPIRHAFFAVFIALPIVALGLLANIGIL
                     PIALACGAVIAAALIGQLGAGLALAWLVEAVLLFLFMPGLAVLYALLGVWLWVRDVRP
                     EQAALAMAMPVAAPFGLAPALILTAAAVEGLMGIVTVAWGGLMTVIVAIAMGRQAFGP
                     FVETGVILEQQSLFDPLRAAQTTSAFQSVLVQPNDRLGPIGAVLDPATLWSQLAGLVS
                     RLAGADATAIATVLAWTAAALVVWSVTRVLRTFFDALLRRPTRWFSLYVFAQAVGAVA
                     GASILFMLFITWGPLANAARGSQNTMMVAAFVGAIIATAASVVISATQRPEQVEEAAP
                     PVAARRLPVR"
BASE COUNT          870 a         1845 c         1869 g          763 t
ORIGIN      
        1 ttggcacagc gaacgtcgca cttcgcgatc tcggcgacca tcgcatccca ttgctgcgcg
       61 cggactacgc tggcgacggc cgctaccttc tcgacgccgt cacgatgatc gaactccagg
      121 agcctggggt ccgtcgcccc gcaatcgacg catggatggt cgcggaagta ctcgagaagg
      181 aaccgcgcga agcgatccct ctccctcgcg ttccggcggc ctgccttatc caggtacgcg
      241 ccgcgattgc ggcggtaatg ctccttcgca tacgcgcgtt gacaggatcg gcacttcgtc
      301 gaccgctgtc cggtgcgctt gttcttgagc gcgaactcgc tcagcggttg gggcacgccg
      361 cacgtgcaac atgttcgagt cccctcgggt gggtacacga gctcgagttg cacgtgaaca
      421 ggcagtggcg aagacagagc gttccccatc gctgccatcg tcgctctgcg ccatgcgaac
      481 tgtgacgccg tccggaggcg gtgacagttg acgcagcgga catcgcactt cgcgatctcc
      541 agctggaccg ccggccacgg cttcctcgtg gcgggcttcg cgacctccag ccgtttggtg
      601 gccggatccc gatgatcgaa gtcgaggacg acgggatcgg tctcgccaca gtcgatgcac
      661 ggatgagcta gcaggtattc gaggagtgat tcgcggttgc gctcccgatg tcccttcacg
      721 cgaagccgct cgttctcgat gtagttcggc ttcgtccgca ggtaatgctg ccgtcgatac
      781 gccgcgtggc aggcgcggca atagctctgt cgcgtgccgt tcgcgatcga ggcgaacgcg
      841 aactcggatt ccggcttgag gaccccgcat ttggcgcagc ggcgcacgtc gtccctcgtc
      901 attccgccgc gcgccggcca cgcgtgtgtc tagaacacct gttctatcac atccgagacc
      961 gcgtgtgaag ccccatcctt cacgtcggtg aagttcgagg gcatcgaggt ccagcgcgcg
     1021 atggtggtct acgcgcaccc cgacgacgcg gagttcggcc tcgccggcac catcgcgaaa
     1081 tgggcgcgcg cgggcgtcga gatcacgtac tgcatggtca cgaacggagc gtcgggctcg
     1141 gacgaccccg acatcacgcg cgaccagctg cgcgacatcc gctacgccga gcagcgcgag
     1201 gcggcgaagg tcctcggcgt gaagcactgc gtgttcctcg gctacgagga cggatacctc
     1261 tatccgacgc tcgaggtgcg gagggacgtg gcgcggcaga tccgcatcca ccggccggac
     1321 gtcctcatca cgatggaccc gacgatgcgc atcgcagagg acttctacgt gaaccacccc
     1381 gaccacatcg cggcgggcga ggtcgcgctc cgctcgatca accccgacgc gtcgacgcgg
     1441 cagatgttcc cggagctgtg gcgcgacgag cacctcgagc cgcacaaacc ggcgctgctc
     1501 atgctgcagt ccttcggcgc gggcggcacg gtcgtggaca tcagcagcac cctcgagacg
     1561 aaggtgaagg cacttctgct gcatcgctcg cagctctggc ccggcgccga ggagctggtg
     1621 cgcgacttcg cgtcgcgcat cggaaaggcc ggcggctaca aggcgggcga atccttccgc
     1681 gtcgtgcgcc tcgagcgccc cgaggagccg ccgaaggcgt cgcgcggcaa gcgcggcgtc
     1741 cagaggcatc ggccgacgtc cacgcggtcg cgcacgaagg gcacgccctc gcgccgcaag
     1801 cgctcgccct gatccgggaa cgacgccgcg agtgacccgt cggcgtggat cacgcggtgg
     1861 cacggcaccg tccgatccgc acagtcgcgc atgacgttgc cgacggcgcg cgcggcgccg
     1921 ggccggccgg acacgaccgc cacgaggccg tagctcgcga cgcgtccgcg cgggatcgcg
     1981 tgcgcgacgt cgatcacgcg ccgatgccag cggcggtccg ccgtcgatac gttgggcacg
     2041 gcgcgagtct ggcacgaccc ctgcgttggc gggccggcat ggcccttccc gcacctgagc
     2101 tcctcgacgc cgcgaaggat gccggccttc ggtacgcgac cgacgaccgc ccgggtatcc
     2161 gccggcaccg cgcgggccac ggcttccggt acgtcggggc cgacggcgag ccgatcgacg
     2221 acgagaagga gctcgcgcgc atccggcgcc tggcgatccc gcccgcctgg acggacgtct
     2281 ggatctgtcc ggacgcgcgc ggccacatcc aggcgacggg tcgcgacgcc aagggccgga
     2341 agcagtaccg ctatcacgag cgcttccgcg agatccgcga cgaggccaag tacgagcgcg
     2401 tgctggattt cgcccgagcg ctgccgaaga tccgcgagcg ggtggacgcg gacctgcgtt
     2461 cgccgaagct cacgcgcgag cgcgtgatcg cgacgatcgt gcggctcctg gagatcacgc
     2521 tcatccgcgt cggcaacgag gagtacgcgc gtgcgaacaa gagctacggc ctcacgacgc
     2581 tgcgcgagaa gcacgtcgag gtcgacggct cgcggatccg cttccgcttt cgcgggaaga
     2641 gcggcaagga gcacgacgtc gagctgtcgg atcgccgcgt cgcgcgcgtc ctccgcgcgc
     2701 tgcaggagct ccccggccag gagctcttcc gctacgtcgg cgacgacggc gatgtccgca
     2761 cggtgagctc cgaggacgtc aacacctatc tgcgcgagat cgtcggcgcg gacttcacgg
     2821 cgaaggactt tcgcacctgg gcgggcacgg tgctggcggc ggtggcgctc ggcgcgcagg
     2881 atcacatcga gaaccagcgg atggcgaaga agaacgtcac cacggcgatc gaacaggtcg
     2941 cagctcgact cggcaacacg cgcacggtgt gccgcaagtg ctacgtgcat ccggcggtcg
     3001 tcgacgcgta tctcgacggg gagctcgccc gcgcgatgca gggtcgcgcc gagggcgagc
     3061 cggccgacga caacgcgctc accgcggacg agaagctggt gctcgcgttc ctcgaacgcc
     3121 gcctcgcgaa ggccgcgcgg cccgcctccg ctgcctagac cggcgcttcg gccgtagctc
     3181 ggctagcgga cgggcagacg gcgcgcggcg acgggcggtg cggcttcctc cacctgctcc
     3241 gggcgctgcg tcgcgctgat cacgacgctt gcggcggtcg cgatgatcgc gccgacgaag
     3301 gccgcgacca tcatggtgtt ctgcgacccg cgcgcggcgt tcgcgagcgg accccaggtg
     3361 atgaagagca tgaacaggat gctcgcgccc gcgaccgcgc ccacggcctg cgcgaacacg
     3421 tagagcgaga accagcgggt cgggcgccgc agcagcgcgt cgaagaacgt gcgcagcacg
     3481 cgcgtgaccg accagaccac cagggcggcc gcggtccacg cgagcacggt cgcgatggcc
     3541 gtggcgtccg cgccggcgag gcgggacacc agacccgcga gctgcgacca gagcgtcgcg
     3601 ggatcgagca cggcaccgat cggtccgagc cggtcgttcg gctgcacgag gacgctctgg
     3661 aacgcgctcg tcgtctgcgc ggcgcgcagc ggatcgaaga ggctctgctg ctcgaggatg
     3721 acgccggtct cgacgaacgg gccgaacgcc tgccggccca tcgcgatggc gacgatgacc
     3781 gtcatgagcc cgccccacgc gacggtgacg atccccatga gtccctccac ggccgcggcc
     3841 gtgaggatca gcgcgggcgc caggccgaac ggagcggcga ccggcatggc catcgcgagc
     3901 gcggcctgct cggggcgcac gtcgcggacc cagagccaca cgccgagcag cgcgtagagg
     3961 accgcgaggc cgggcatgaa caggaacagc aggacggcct cgacgagcca tgcgagggcg
     4021 agtcccgcgc ccagctgccc gatcagcgcc gcggcgatca ccgcgccgca ggcgagcgcg
     4081 atcggcagga tgccgatgtt cgccagcagg ccgagcgcga cgatcggcag cgcgatgaac
     4141 accgcgaaga aggcgtgacg gatcggctgt ccgtactcgg acaggagcgc gaccactggg
     4201 ctctgcgtga cggtcgggac gaagtcacgg atcggctcgg tcatcacgcc gacgatgcgc
     4261 gtggtggcga gctcccggtc gaggttctcg atcgagctca gcgcggtcgc gagctcggat
     4321 gccttctggt aacgcagacg cggctccttg gcgagagcct tgagcacgat cgtgtcgagc
     4381 tcgcgcacca ggctctgacg cagatggctc ggcggtacgg gcatgcgcac gacgtggtcg
     4441 cgcagcagga ccgcggcgtt cgcgctcacg aatggcggac gccccacgag catctcgtac
     4501 agcaggagac cgagaccgta cacgtcgctc gtctgcgtcg ccggctttcc ctcgacacgc
     4561 tccggcgcga cgtacatcgc cgtggcgaag agctgcggtg tgtcggcctc ttcctgcggc
     4621 gtccgcgcga tgccgaagtc gcagatcttc gcgtggtcgt tcgcgtcgaa caggatgttc
     4681 gacggtttga ggtcggcgtg gatgatgccg cgcgagtgcg cgaacgcgag cccgttcgcg
     4741 atctcgatcg tgtgcgcggc agcctcgccc accgcgagag gaccgctccg ctcgatgcgc
     4801 tgcttgagcg atccgcccgg caggtgctcc atcacgatga acgggacgcc gtcgacctca
     4861 ccgacatcga agaccgagac gatgctgggg tgggagagcg acgcgagcgc gcgcgcttcc
     4921 ttcacgaaca tcttgcgcag cgtgggatcc gcggcggccg ccgcgtcgag gaccttgatc
     4981 gcgacctcgc ggttcagcgc ggtgtcctgc gcgaggtaga cctgtgagga gcccccggcc
     5041 ccgatcggcg agacgatctt gtagcgcttg tccaggactc gctccacagc cagctccgtc
     5101 atgggcgggc gcgcgccgac tatagcgccg gaccgctgcg cgacagcctg cgtgaacggg
     5161 tttcccatgc gtctcccccg agcgcctgcc tcgtcacccg cagtcaacgc cggagccgtg
     5221 accgctgtta catggcaggc tcggtcggca tgctcccagc caaggttcgc gaccgaatgg
     5281 ataccggaat caggctaagt cggccgaagc ggcgtgacag cgcgaccgag ggcccccgcg
     5341 tagagag
//
LOCUS       DASTZR010000076         2181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_448697, whole genome shotgun sequence.
ACCESSION   DASTZR010000076 DASTZR010000000
VERSION     DASTZR010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448697"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VFV20_01705"
     CDS             complement(<1..555)
                     /locus_tag="VFV20_01705"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEU5287094.1"
                     /translation="MDPVLAAYLEDWLHLAVRWIHIVAAIAWVGASFYFIALDRSLRA
                     PVGGAAGGIGGEAWEIHGGGFYRVEKFAVAPPSLPERLQWFKWEAYLTWLSGFALLVL
                     LYYLDPTTYLIDPAVRALEPWQAVAASLVLLVGGWIVYDQLARRLGTRQRLLAVCLAI
                     FVALVAWACGQLFSARASYIQVGAM"
     gene            complement(559..1149)
                     /locus_tag="VFV20_01710"
     CDS             complement(559..1149)
                     /locus_tag="VFV20_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287095.1"
                     /translation="MPPKPPATEAWRSSITARITAVNDMLDLAVADLTLDQVNRSERK
                     GVLPIAFSLAHVVGSQDRSASRFLANGAPSLWESGDWAQRVKISGGLPFRGTPMEEAE
                     RVRFGDLDAWREYQSGVFAKTESALENAPLALFDRDAFGGERPDLGENSFLGLLVPSG
                     PISVADVCEAYLFQHAARHLGEIEHARALVGLGGIS"
     gene            complement(1163..2038)
                     /locus_tag="VFV20_01715"
     CDS             complement(1163..2038)
                     /locus_tag="VFV20_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEU5287096.1"
                     /translation="MRLREGDVLIQERGGDKDAYLLIDGSLRIVAHGEGRTLALVGAP
                     ALVGEMAVVTDAERSATVLADTACVVLRLGGRELRELMARQPLFATAMRERTDLLLAD
                     AFLKRHSPLRDLPADIVGMLVSRLRPRDLAPDQLIAGGDDDLYLVRRGAVERTRDGER
                     TGPGAFVQRERGERYAAVGETSIYELRMSDVATEIMRHQERLRAIASRLVDRSRVRAL
                     AGVVVLPAPDLGGALVHDATHRAVVSDPVGALVPSLDGRTDLATLVRGSGHPRGAIVE
                     GLAMLIAAGLAEARD"
     gene            1943..>2181
                     /locus_tag="VFV20_01720"
     CDS             1943..>2181
                     /locus_tag="VFV20_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287097.1"
                     /translation="MRDDAKRSVDEQVRILVAAALLDEDVSLAQPQLACVGAKPFTRL
                     GFEQQERPVAQGARQRSSRCETSGTDGSQPSFTRER"
BASE COUNT          352 a          780 c          774 g          275 t
ORIGIN      
        1 catcgcgccg acctggatgt acgacgcgcg cgcgctgaag agctggccgc acgcccacgc
       61 gaccagcgcg acgaagatcg cgagacagac cgcgaggagc cgctgccgcg tcccgaggcg
      121 ccgcgcgagc tggtcgtaga cgatccatcc gccaacgagc agcacgaggc tggcggcgac
      181 ggcctgccac ggctccagcg cccggaccgc cggatcgatg agatacgtcg tcgggtccag
      241 gtaatagagg aggaccagca gcgcgaagcc gctgagccag gtgagatacg cctcccactt
      301 gaaccactgc agccgctcgg gcagcgatgg cggcgcgacg gcgaacttct cgactcggta
      361 gaagcctccg ccgtggatct cccaggcctc gccgccgata cccccagccg cgccgccgac
      421 gggggcgcgc agcgagcgat cgagggcgat gaagtagaag gacgcgccga cccacgcgat
      481 cgccgcgacg atgtggatcc agcggaccgc aagatgcagc cagtcctcga ggtaggccgc
      541 gaggacggga tccaacgcct aggagatgcc gccgagcccg accagcgccc gcgcgtgctc
      601 gatctcgccc aggtgacgcg ccgcgtgctg gaagagatag gcctcgcaca cgtcggcgac
      661 cgagatcggt cccgacggca cgagcagtcc gaggaacgag ttctccccga ggtcgggccg
      721 ttcgccgccg aatgcgtcgc ggtcgaagag cgcgagcggc gcgttctcga gcgcgctctc
      781 cgtcttcgcg aagacgccgc tctggtactc gcgccacgca tcgaggtcgc cgaagcgcac
      841 acgctcggcc tcctccatcg gcgtcccgcg gaacggcagc ccgccactga tcttcacacg
      901 ctgcgcccag tcgccgctct cccagaggct cggggcgccg ttcgcgagga agcggctcgc
      961 cgagcggtcc tggcttccca cgacgtgcgc gaggctgaag gcgatcggca ggacgccctt
     1021 ccgctcggag cgattgacct gatcgagcgt gagatcggcg accgcgagat cgagcatgtc
     1081 gttcaccgcg gtgatgcgcg cggtgatgga cgatcgccag gcttcggtcg cgggcggctt
     1141 cggtggcatc ggtccgcaga gtttagtcgc gtgcctccgc caggccggcg gcgatgagca
     1201 tcgcgagccc ttcgacgatc gcgccccggg gatggcccga cccgcgcacg agcgtcgcga
     1261 ggtcggtccg gccgtcgagc gacggcacga gcgcgccgac cggatccgag acgacggcgc
     1321 gatgcgtcgc gtcgtgcacg agggcgccgc cgagatcggg ggccggcagc accactacgc
     1381 ccgccagcgc ccgcacgcgg cttcggtcca ccagacgcga cgcgatggcc cgcaggcgct
     1441 cctgatggcg catgatctcg gtcgcgacgt ccgacatacg cagctcgtag atggacgtct
     1501 cgccgacggc cgcgtatcgc tcaccgcgct cacgctggac gaacgcgccg ggacccgtgc
     1561 gctcgccgtc gcgggtgcgc tccaccgctc cgcgacggac gaggtagaga tcgtcgtcgc
     1621 cacccgcgat cagctggtcg ggcgcgagat ccctcggtcg gagccgtgac acgagcatgc
     1681 cgacgatgtc cgcgggcaga tcacggagcg ggctgtgccg cttcaggaac gcgtcggcga
     1741 gcagcagatc ggtgcgttcc cgcatcgccg tcgcgaacag cggctgacgc gccatgagct
     1801 cgcgcagctc ccgaccgccg agacgcagga cgacgcacgc cgtatcggcg aggacggtcg
     1861 cgctgcgctc ggcgtcggtc acgacggcca tctcgcccac gagcgccggc gcgccgacca
     1921 gcgcgagcgt gcggccctcg ccgtgcgcga cgatgcgaag cgatccgtcg atgagcaggt
     1981 acgcatcctt gtcgccgccg cgctcctgga tgaggacgtc tccctcgcgc agccgcagct
     2041 cgcgtgcgtg ggcgcgaagc cgttcacgcg cctcgggttc gagcagcagg aacggcccgt
     2101 agcgcaggga gctaggcagc ggtcatctcg ctgcgagact agcgggacgg acgggtctca
     2161 gccgtcattc acgcgggagc g
//
LOCUS       DASTZR010000077         2113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1127676, whole genome shotgun sequence.
ACCESSION   DASTZR010000077 DASTZR010000000
VERSION     DASTZR010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127676"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VFV20_01725"
     CDS             <1..215
                     /locus_tag="VFV20_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5287098.1"
                     /translation="VVATLDVLSHGVAPLGAIVAAVIAESCGVRTAIAVGWAGMFASV
                     LWLVFSPLPRLRTLDAWRAELGATRV"
     gene            933..1005
                     /locus_tag="VFV20_01730"
     tRNA            933..1005
                     /locus_tag="VFV20_01730"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:967..969,aa:Gly,seq:tcc)
     gene            1114..1707
                     /locus_tag="VFV20_01735"
     CDS             1114..1707
                     /locus_tag="VFV20_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287099.1"
                     /translation="MLVLLIAAGALSVCGFGAREQPSPTPTSTPTATPTPTPRDLTGV
                     PEACRSSVHGVAFAEGSLGTRVREANAWSAEHPGETGSLVVTDDVMRDAAAREDWSVP
                     VRDIAVTTDDAGFHLSATASVFGRYTIRALLVPQAVDGTLRISVRDLDTGGLPAFIRP
                     AVESAIASATDTSRWGLRLTVDGVTTRQGCAVVWGRG"
     gene            complement(1704..>2113)
                     /locus_tag="VFV20_01740"
     CDS             complement(1704..>2113)
                     /locus_tag="VFV20_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287100.1"
                     /translation="LLSGWRESKPAPRGWKPYPYTRDDPLGDFLSALRRVRELPVKRV
                     LPAHGEPFDDLKGRVDEILAHHDARLDAVLAALGSRERDAYSVARDLFPVLRSAHEER
                     FALAETLAHLRHLERDGRVQMLDGARAAWKVAA"
BASE COUNT          336 a          735 c          703 g          339 t
ORIGIN      
        1 gcgtggtcgc gacgctcgat gtactgagcc acggcgtcgc gccgctcggc gcgatcgtgg
       61 cggcggtcat cgcggagtcg tgcggcgtgc gcaccgcgat cgccgtcggc tgggccggga
      121 tgttcgcatc ggtcctgtgg ctggtgttct cgccgctccc acgattgcgg acgctcgatg
      181 cgtggcgcgc ggagctgggt gcgacgcgcg tctgacggcg cgcaggggct tgacgagggt
      241 agaacgggtg ttctaagatg ggatatggcg agcgcgcgcg atcagcatcc gtacaacgtc
      301 gcgtactacg cgaggaatcg cgacgcggag atcgcgcgcg tgacagctcg tcagcaggca
      361 acgctcgcgt ggctgcgcga gctgcgcacc gtcccttgcg cggattgcgg cggcatcttc
      421 ccaccgtacg tgatgaactt cgaccatcgc gatccgaaga cgaagttatt caacctcgga
      481 gcgggaagct cgctgctcaa gaaccgagcg ctgctcgagg cggaggtcgc caagtgcgac
      541 atcgtctgcg tgaactgcca tcggatccgg acccacacgg cattcatgaa cggcacgatg
      601 cgaccgctcg cgctgaccag gcgggcggag ggcggcacga accaccagat caggtcacgg
      661 gcgcgattca ggcagacgcg cgaggtccac gctgagctgc tccgttccct tcgcgtcgct
      721 ccgtgcgtcg actgtcgccg cacctttccc tggttcgtga tggaattcga tcacctcgac
      781 gcatccaaca agaccgccct tgtcacgcga atgatcggac gggtgcggac cgcgacgctg
      841 ctcaacgaga tagcgaagtg cgatatcgtg tgtgcgaact gccatcgcga acggacatac
      901 catcggcgct catgcatagc gtccaactgt ccgcgggtgt ggtgtagtgg tcatgcacgc
      961 gacccttcca aggttgaagg acgggttcga ttcccgtcac ccgctcagca gcaaccccgt
     1021 ctgatagaag agtccgcgac tccataccga tacgccgcct gatcgctcgc ggcacccatc
     1081 ctgcatcggc atgcgccgat gttccgttct accgtgctcg tccttcttat cgccgcgggc
     1141 gcactcagcg tgtgcggatt cggcgctcgc gaacagccgt ccccgacgcc gaccagcacg
     1201 cccacggcta cgcccacgcc aacaccacgc gatctcaccg gcgtgccgga ggcgtgccgc
     1261 tcctccgtgc acggcgtggc cttcgccgaa ggctcgctcg gcacacgcgt ccgcgaagcc
     1321 aacgcatggt ccgcggaaca tccgggtgag accggctcgc tcgtcgtcac ggacgacgtc
     1381 atgcgcgacg cggcggcgcg cgaggactgg tccgtgccgg tacgcgacat cgccgtcacc
     1441 acggatgacg ctggcttcca tctgagcgcg accgccagcg tcttcggccg gtacacgatc
     1501 cgcgcgctcc tcgtgccgca ggcggtggac ggaacgctgc gcatcagcgt tcgcgacctc
     1561 gatactggcg gacttccggc attcatccgc cccgccgtcg aatcggcgat cgcgagcgcg
     1621 accgacacga gccgctgggg ccttcgcctc accgtcgacg gcgtcaccac gcgacaggga
     1681 tgcgcggtcg tctggggtcg aggctaggcc gcgaccttcc acgccgcgcg cgcgccgtcg
     1741 agcatctgca cgcgcccgtc gcgctcgaga tggcgcagat gcgcgagcgt ctccgcgagg
     1801 gcgaagcgct cctcatgcgc cgaccgcagg accgggaaca gatcgcgcgc caccgagtac
     1861 gcgtcgcgct cgcgcgagcc gagcgccgcg agcaccgcgt cgaggcgcgc gtcgtggtgc
     1921 gcgaggatct cgtccacccg tcccttcagg tcgtcgaacg gctcgccgtg cgccggcagg
     1981 acccgtttca ccgggagctc gcggacgcgg cgcagcgccg acaggaagtc accgagcgga
     2041 tcgtcgcgcg tgtacgggta aggcttccat cctcgaggtg cgggtttcga ttcccgccac
     2101 ccgctcagca gct
//
LOCUS       DASTZR010000078         4807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_944829, whole genome shotgun sequence.
ACCESSION   DASTZR010000078 DASTZR010000000
VERSION     DASTZR010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944829"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VFV20_01745"
     CDS             <1..542
                     /locus_tag="VFV20_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287101.1"
                     /translation="TSDARAILVTLTLKSVKPELHVTVEALDDLSEPHLRRAGADDVV
                     VSGEFNGFLLSSTAIAPGIAQVVRPLLSLSGSELRRVGVPSDLAGKSYGEVAAALRAR
                     DGFQAIAVVHEDRGLTLDQLLSDDTSVVDHFIRAQFSEAGREFLRYEAGGTRAIVNPP
                     DAYEVGPKDAIVGIPRGAA"
     gene            542..1039
                     /locus_tag="VFV20_01750"
     CDS             542..1039
                     /locus_tag="VFV20_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEU5287102.1"
                     /translation="MPHEVLRAIPIFHGLTEAQLDAVAAVLVPRSVRGGEAIFAEGER
                     SSAMYVLTSGAVGTSKRLGLAAQVPAAAAPQKVLVHLRAPQFFGEMGLLSELDRSATI
                     TTEGDCELLELRREDFERLAAQDAALGYHLVRNIAVVLAERLRRTDLDVLKLTTALSL
                     ALGNR"
     gene            1036..2793
                     /locus_tag="VFV20_01755"
     CDS             1036..2793
                     /locus_tag="VFV20_01755"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEU5287103.1"
                     /translation="MTAHTLRVLATVLLALSALLPMAESSARAADGGWTITSFDVQIE
                     IQRDGRIVVDENLAVDFGPLEKHGIFRYVPVKYEWPSEKRKIRVYELQVLSVMDAKGR
                     PWRYETSGQDANVEIKVGDPDRTLSGAQTYRIRYAVKGVLNSFADHDELFWNVTGGDW
                     GVPILRSSATVRAPAALSRSACFVGFPGATAKCGSTQTLGTATTFTTGRSLAPGEQLT
                     IVAALPKGVVSEPTPILQDRPREFSEFFDPTPVWLALAALVAILGLAWVFWRWFTAGR
                     DDPDRVTIVPEYEPPAKTRPAQLGLLVDERADTLDVTASIVDLAVRGYLTITEIPKEG
                     LFGSRDWLLAQKREADPDLLEYERTILDGLFDDGPEVKLSALKRHFYTTLGKAEKELY
                     TDATTRGWFPADPSRVRATYAVAGVGLVVLAGAIAFGLGYLAGGGLVGIGAAVPAIAL
                     IAASPLMPRRTKAGAELARRALGFRQYMEVAEKDRQRFAEKEHIFAEYLPFAIVFKCV
                     DQWAKAFQGIDLREATSTWYTGNLATFNAMDLSRDLSSFSGQLSTAIASTPGGSGSSG
                     FGGGGGAGGGGGGGGGGSW"
     gene            2847..3065
                     /locus_tag="VFV20_01760"
     CDS             2847..3065
                     /locus_tag="VFV20_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287104.1"
                     /translation="MPLPRDERADDDRVVGNYALGSLVLLCGLFGMYFGIRNPRGDAW
                     GPGWKYVLGGVIFAILGLVFIGAEVGWL"
     gene            complement(3112..3184)
                     /locus_tag="VFV20_01765"
     tRNA            complement(3112..3184)
                     /locus_tag="VFV20_01765"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3149..3151),aa:Val,seq:cac)
     gene            complement(3215..3523)
                     /locus_tag="VFV20_01770"
     CDS             complement(3215..3523)
                     /locus_tag="VFV20_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287105.1"
                     /translation="MARRHRTRTRTRRTPEVGRGSVVPARAEREEARAPRPSTHRPSR
                     GSRTGYARAVGAPSQALERAAVMERGFVLKDFRRLGLVVAVALALLIVAGFLESVLIK
                     "
     gene            3565..4089
                     /locus_tag="VFV20_01775"
     CDS             3565..4089
                     /locus_tag="VFV20_01775"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HEU5287106.1"
                     /translation="MTFDERTLSLLVVALGGAVLLLVVWVALLQRSESRLRRRLRRVL
                     PGGEQVSLDEIMSRQLERIEQLDTRVDALNKLQQELEAITQRTLQKVGVVRYNPFADT
                     GGDQSFAIALLDPNGTGLVISSLHSRTETRVFAKPVQGGRSRYPLSDEEQDAIKRALT
                     PSGSAERVSPTARD"
     gene            4086..>4807
                     /locus_tag="VFV20_01780"
     CDS             4086..>4807
                     /locus_tag="VFV20_01780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEU5287107.1"
                     /translation="MNRERALAIVNPVAGKGTGAKIVRRLAADLAKAGLHVDIVSTPG
                     PSEAARLASAAVEDGYERVISVGGDGTANEVANGLIGSDVALGLYPIGSGNDFARALG
                     YPRKRSKIAAFLAQARPRRIDVGEVNGRIFLNAAGVGIDGHVAERIIASTRVVGPTLG
                     YFVGALVSIATYRPRTMRVRVGGTLREGKHLIVVAANGTHFGSGMHVAPDAKIDDGQF
                     EIIVGGDLGRWASLVALGKLYRG"
BASE COUNT          701 a         1650 c         1735 g          721 t
ORIGIN      
        1 ccacgagcga cgcgcgcgcg atcctcgtga cgctcactct gaagagcgtg aagcccgagc
       61 tccacgtcac ggtggaggcg ctcgacgacc tgagcgagcc gcacctgcgt cgtgcgggcg
      121 ccgacgatgt cgtcgtcagc ggcgagttca acggcttcct gctgtcgtcc acggcgatcg
      181 cccccggcat cgcgcaggtc gtgcgcccgc tgctctcgct ctcggggagc gagctgcggc
      241 gcgtcggggt gccgagcgat ctcgcgggca agagctacgg cgaggtggcg gcggcgctgc
      301 gcgcgcgtga cgggttccag gccatcgcgg tggtccacga ggaccgcggt ctgacgctgg
      361 accagctcct gtcggacgac acgtcggtcg tcgaccactt catacgcgcg cagttcagcg
      421 aagccggacg ggagttcctc cgctacgagg ccggcgggac gcgcgcgatc gtgaacccac
      481 cagacgcgta cgaggtcgga ccgaaggacg cgatcgtggg cattccgcgg ggcgccgcgt
      541 gatgccgcac gaggtcctgc gggcgatccc gatcttccac ggcctcaccg aggcgcagct
      601 cgacgcggtc gccgccgtgc tcgtgccgcg aagcgtgcgt ggcggcgagg cgatcttcgc
      661 cgagggggag cgctcgagcg cgatgtacgt ccttacctcc ggcgcggtcg gcacctcgaa
      721 gcggctgggg ctcgccgccc aggtacccgc ggccgcggca ccgcagaagg tgctcgtcca
      781 tctgcgagcg ccgcagttct tcggcgagat gggcctcttg tccgagctgg accgctcggc
      841 gacgatcacg acggagggcg actgcgagct cctcgagctg cggcgcgagg acttcgagcg
      901 gctcgcggcg caggacgcgg ctctcggcta tcacctggtg cgcaacatcg cggtggtgct
      961 ggcggaacgg ctgcgccgga cggacctcga cgtgctcaag ctgaccacgg cccttagcct
     1021 ggcgctcggc aaccgatgac cgcccacacg ttgcgcgtgc tcgcgaccgt gctcctcgcg
     1081 ctgtcggcgc tgctcccgat ggccgagtca tccgcgcgcg cggccgacgg tggctggacg
     1141 atcacgagct tcgacgtgca gatcgagatc cagcgcgacg gccgcatcgt ggttgacgag
     1201 aatctcgccg tcgacttcgg cccgctcgag aagcacggca tcttccgcta cgtcccggtg
     1261 aagtacgagt ggccgagcga gaagcggaag atccgcgtgt acgagctgca ggtgctctcg
     1321 gtgatggacg cgaagggtcg cccgtggcgc tacgagacct cagggcagga cgcgaacgtc
     1381 gagatcaagg tcggcgatcc cgatcgcacg ctgagcggag cgcagacgta ccgcatccgc
     1441 tacgcggtga agggcgtgct caacagcttc gccgaccacg acgagctctt ctggaacgtg
     1501 accggcggcg attggggcgt gccgatcctg aggtcctcgg cgacggtccg cgcgccggcg
     1561 gcgctgtcgc ggagcgcgtg cttcgtcggc ttcccgggcg cgacggcgaa gtgcgggagc
     1621 acgcagaccc tcgggacggc caccacgttc accaccggcc gatcgctcgc gcccggtgag
     1681 cagctcacca tcgtcgcggc cctgccgaag ggcgtcgtgt ccgagcccac gccgatcctc
     1741 caggaccggc cacgcgagtt ctccgagttc ttcgacccca cgccggtctg gctcgcgctc
     1801 gccgccctcg ttgcgatcct gggtctcgcc tgggtgttct ggcgctggtt caccgccggg
     1861 cgcgacgacc cggaccgggt gacgatcgtg ccggagtacg agccgccggc gaagacccgg
     1921 ccggcacagc tcgggctgct cgtcgacgaa cgcgccgaca cgctcgacgt caccgcgtcg
     1981 atcgtcgatc tcgccgtgcg cgggtatctc acgatcaccg agatccccaa ggagggcctc
     2041 ttcggctcgc gcgactggct gctcgcccag aaacgcgagg ccgatcccga cctgctcgag
     2101 tacgagcgca cgatcctcga cgggctgttc gacgacggtc cggaggtgaa gctctccgcg
     2161 ctgaagcgcc acttctacac gacgctcgga aaggccgaga aggagctcta caccgacgcg
     2221 accacgcgcg gctggttccc ggccgacccg tcgcgcgtcc gcgccacgta cgccgtcgcg
     2281 ggcgtcggtc tggtggtgct cgccggggcg atcgcgttcg ggctcggcta tctcgcgggc
     2341 ggaggcctgg tggggatcgg tgcggccgtc ccggcgatcg ccctcatcgc ggcgtcaccg
     2401 ctcatgccga ggcggaccaa ggccggcgcc gagctcgcga ggcgcgcgct cggcttccgc
     2461 cagtacatgg aggtcgccga gaaggatcgg cagcgcttcg cggagaagga gcacatcttc
     2521 gccgagtacc tgccgttcgc gatcgtcttc aagtgcgtgg accagtgggc gaaggcattc
     2581 caggggatcg acctgaggga ggccacctcg acctggtaca ccggcaacct cgcgacgttc
     2641 aacgcgatgg acctgtcgcg agacctctcg tcgttctccg gccagctctc gaccgcgatc
     2701 gcgtcgaccc caggcggcag cggctcgagc ggcttcggcg gcggtggggg agcgggcggc
     2761 ggcggtggcg gaggcggcgg agggagctgg taggcgcggc tgcgcccgac aggaccgagg
     2821 tcgagtgcct cgacgtccgg tgaccgatgc cgctacccag agacgagcgc gcggacgacg
     2881 atcgtgtcgt gggcaactac gcgctgggga gcctggtgct cctctgcggt ctcttcggga
     2941 tgtacttcgg catccgcaac ccgcgtggag acgcgtgggg gccgggatgg aagtacgtgc
     3001 tgggcggcgt gatcttcgcg atcctcggcc tcgtgttcat cggcgccgaa gtcggctggc
     3061 tctagccgca ccgccaccga cggatcctga cgagccgtat cgctcctcgt gtgggctcgg
     3121 cgggattcga acctgcgacc cctcggatgt gaaccgagtg ctctaaccac tgagccacga
     3181 gccccgacct caagagttta ccggggcggg cgcgctactt gatgagcacg ctctccagga
     3241 agccggccac gatgagcaag gcgagcgcga ccgcgacgac gaggcccaga cgccggaagt
     3301 ccttcagcac gaagccccgc tccatgaccg cggcgcgctc gagcgcctgc gacggcgcgc
     3361 cgaccgcccg ggcgtagcct gttcggctgc cacgcgaggg gcgatgcgtc gacggacgtg
     3421 gcgcgcgtgc ctcctcgcgc tccgcgcgag cgggcaccac gctgccgcgg ccgacctcgg
     3481 gggtgcggcg tgtgcgcgtt cgggtgcggt gtctcctcgc catctcggaa gcccaagcat
     3541 agcgttccgg gtatcgtccg cgcggtgacg ttcgacgaac ggaccctgtc gctcctggtc
     3601 gtcgccctcg gtggcgcggt gctcctcctc gtcgtgtggg tggcgctgct gcagcgctcc
     3661 gagtcccgtc tgcgccggcg gctgcgtcgg gtgctgccgg gcggcgagca ggtgagcctc
     3721 gacgagatca tgtcgcggca gctcgagcgg atcgagcagc tcgacacacg ggtggacgca
     3781 ctgaacaagc tgcagcagga gctcgaggcg atcacgcagc ggacgctcca gaaggtgggc
     3841 gtcgtccgct acaacccatt cgccgatacg ggaggcgacc agtccttcgc gatcgcgctg
     3901 ctcgatccga acggcaccgg actggtgatc tcgagcctgc acagccgcac cgagacgcgt
     3961 gtgttcgcga agccggtgca gggcggccgc tcgcgctatc cgctgtcgga cgaagagcag
     4021 gacgccatca agagggcact cacgccgtcc ggcagcgctg agcgtgtctc gcccacggcg
     4081 cgcgattgaa ccgagagcgc gctctcgcga tcgtgaaccc ggtcgcgggg aagggcaccg
     4141 gcgcgaagat cgtgcgccgg ctggccgccg atctcgcgaa ggccggattg cacgtcgaca
     4201 tcgtcagcac gcccggtccc tcggaggccg cgcgcctcgc gagcgccgcg gtcgaggacg
     4261 gttacgagcg cgtgatctcg gtgggcggcg atgggacggc gaacgaggtc gcgaacggtc
     4321 tcatcggcag cgatgtcgcg ctcggtctgt atccgatcgg atccggcaac gacttcgccc
     4381 gggccctcgg ctacccgcga aagcgctcca agatcgcggc gttcctcgcc caggcgcgtc
     4441 cgcggcgcat cgacgtgggc gaggtgaacg gtcgcatctt cctgaatgcg gccggtgtcg
     4501 gcatcgacgg ccatgtcgcg gagcggatca tcgcgagcac gcgcgtcgtc gggccgacgc
     4561 tcggctactt cgtcggcgcg ctcgtctcga tcgcgaccta tcgtccgagg acgatgcgcg
     4621 tgcgcgtcgg cggcacgttg cgcgagggga agcacctcat cgtcgtcgcc gccaacggga
     4681 cgcatttcgg cagcggcatg cacgtcgcgc cggacgcgaa gatcgacgac ggccagttcg
     4741 agatcatcgt gggcggcgac ctcggccgat gggcctcgct cgtggcgctg gggaagctct
     4801 atcgcgg
//
LOCUS       DASTZR010000079         2451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_369291, whole genome shotgun sequence.
ACCESSION   DASTZR010000079 DASTZR010000000
VERSION     DASTZR010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369291"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VFV20_01785"
     CDS             complement(<1..365)
                     /locus_tag="VFV20_01785"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HEU5287108.1"
                     /translation="MIGERLLAARAITEAQLEEALAWQRASGGLLGEILLKLHYITDE
                     AIARALASQAGVPFRAINGVEPDPAAVALLPEAIARRYLVAAVALEHDTLRVVQANPF
                     DVVGLDVVQRACGRRVETE"
     gene            complement(362..1774)
                     /locus_tag="VFV20_01790"
     CDS             complement(362..1774)
                     /locus_tag="VFV20_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020887531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU5287109.1"
                     /translation="MMFQILVVDDDEQMQFFLKEALERRQYVVTIAPTGAAAIERVAA
                     QRFDLVLLDVRLPDANGVELVERVQKIDRELPIIIMTAHGTRDTAIEAMRHGAYDYFT
                     KPFRLDELEIVVRRALEKGRLLDEIQQLRQKPAGARRRGRLIGSSGAMHEVFRLIDRA
                     GPTDSTVLILGESGTGKELIAEALYEASGRRDKPFVKLNCAAIPETLLESELFGHERG
                     AFTGAVTRKLGKFESADGGTLVLDEIGDMTLATQAKILRVLQERELQRVGGTQTVRVD
                     VRILASTNKDLERAVREGQFRDDLYYRLNVVTIHVPPLRERRDEIPELADHFLAEANA
                     QVGRSIRRLAPDTLGALMEYHWPGNVRELKNAIERAVVVNDGDVLELTSLAPPIRPPD
                     AGAGLAGYRRWEGLDGLPLDEKVAQLERAFVVDALTRAQGVQAAAARLLGVTERSVWH
                     LVKKHRIEVSRIKERSETAS"
     gene            complement(1771..>2451)
                     /locus_tag="VFV20_01795"
     CDS             complement(1771..>2451)
                     /locus_tag="VFV20_01795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5287110.1"
                     /translation="HELRNPLASLRGLAELLGRDFDAADPRSRYVATMLTAIDRLDHM
                     IEDLLLFSSPAPALTEHIDLASLVRELALFIGHGTPDRNVRLEVAPAAAGISVVANRD
                     RVVHAFTNILRNAVQATPDGGVVTARVDRTDREAIVCIHNTGSFIPPDVRAQLFVPFF
                     TTKSGGTGLGLAIARQFITAAGGRIDVDSDQADGTTFSVALPLGATTGTAAPAENTIT
                     FSTHPSRA"
BASE COUNT          354 a          860 c          849 g          388 t
ORIGIN      
        1 cactccgtct cgacccgccg cccgcaggca cgctgcacga cgtcgagccc gacgacgtcg
       61 aaggggttcg cctggacgac gcgtagcgtg tcgtgctcca gcgcgacggc ggcgacgaga
      121 taccgcctcg caatcgcttc cggcaggagc gccacggccg ccgggtccgg ctcgaccccg
      181 tttatggcgc ggaaggggac gcccgcttgc gaggcgaggg cccgcgcgat cgcctcatcg
      241 gtgatgtagt gcagcttgag caggatctcg ccgagcaggc cgccggacgc gcgctgccag
      301 gcgagcgcct cctcgagctg cgcttccgta atcgcgcgcg ccgcgagcag ccgctctcca
      361 atcacgaggc cgtctccgat cgttccttga tgcgcgatac ctcgatgcgg tgcttcttca
      421 ccaggtgcca gacgctgcgc tcggtgacgc cgagcaggcg cgccgcggcc gcctggacgc
      481 cctgggcgcg cgtcagcgcg tccacgacga acgcgcgctc gagctgcgcg accttctcat
      541 cgagcggcag gccgtcgagg ccctcccacc ggcggtaccc ggcaaggccc gcacccgcat
      601 ccggcggcct gatgggaggc gcgaggctcg tgagctcgag gacgtccccg tcgttcacga
      661 cgaccgcgcg ctcgatcgcg ttcttcagct cgcgcacgtt cccgggccag tggtattcca
      721 tcagcgcgcc gagcgtgtcc ggtgcgaggc ggcggatgct gcggccgacc tgtgcgttcg
      781 cttcggcgag gaagtgatcg gcgagctccg ggatctcgtc gcgccgctca cgcagcggcg
      841 gcacgtggat cgtgacgacg ttcagccggt agtacagatc gtcgcggaac tgcccctcgc
      901 gcacggcgcg ctcgaggtcc ttgttcgtcg aggcgaggat gcgcacgtcc acgcggacgg
      961 tctgcgtgcc gccgacgcgc tgcagctcgc gctcctgcag cacgcggagg atcttggcct
     1021 gcgtcgccag cgtcatgtcg ccgatctcgt cgagcacgag ggtgccgcca tccgccgact
     1081 cgaacttgcc cagcttgcgg gtgaccgcgc cggtgaaggc gccgcgctcg tggccgaaca
     1141 gctcggactc caggagcgtc tcgggaatcg ccgcgcagtt caacttgacg aacggcttgt
     1201 cgcgacgccc cgaggcctcg tagagcgcct ccgcgatgag ctccttgccc gtgccgcttt
     1261 cgccgaggat caacactgtg gagtccgtgg gccccgcgcg atcgatcagc cggaagacct
     1321 cgtgcatcgc gccgctcgat ccgatcagcc gcccgcggcg ccgcgcgccc gcgggcttct
     1381 gccgcagctg ctggatctcg tcgaggagcc gccccttctc gagggcgcgc cgcaccacga
     1441 tctcgagctc gtcgagccgg aacggcttcg tgaagtagtc gtacgcgccg tgccgcatcg
     1501 cctcgatcgc cgtgtcgcgc gtgccgtgcg ccgtcatgat gatgatcggc agctcgcggt
     1561 cgatcttctg gacccgttcg acgagctcca ccccgttggc gtccggcagc cgcacgtcga
     1621 gcagcacgag gtcgaaccgc tgcgcggcaa cccgctcgat ggcggcggcg ccggttggcg
     1681 cgatggtgac gacgtactgc cgccgttcca gcgcctcctt caggaaaaac tgcatctgtt
     1741 cgtcgtcgtc gacgacgagt atctgaaaca tcatgccctc gatggatgcg tgctgaacgt
     1801 gatggtgttc tccgcgggcg cagccgtgcc ggtcgtggcg ccgagcggca gggccacgga
     1861 gaacgtcgtc ccgtcagcct gatcgctgtc aacgtcgatt cgccctcccg ccgccgtgat
     1921 gaactgccgc gcaatcgcga gcccaagccc cgttccgccc gatttcgtcg tgaagaacgg
     1981 gacgaagagc tgcgcgcgca cgtccggcgg gatgaaggaa ccggtgttgt ggatgcacac
     2041 gatcgcctcg cgatccgtcc tgtccacgcg ggccgtcacg acgcctccgt ccggtgtcgc
     2101 ctggacggcg ttgcgaagga tgttcgtaaa cgcgtgcacg acgcgatcgc ggttcgcgac
     2161 gaccgaaatc cctgcggccg caggggcgac ctccagtcgg acgttccggt cgggggtgcc
     2221 atggccgatg aagagcgcca gctcgcggac gagcgacgcg aggtcgatgt gctcggtcag
     2281 cgcgggcgcc ggcgacgaga acagcagcag atcctcgatc atgtggtcga gtcgatcgat
     2341 ggcggtgagc atcgtcgcga cgtagcggct gcgcgggtcc gccgcgtcga aatcgcggcc
     2401 caggagctcg gcgagcccgc gaagcgaggc cagcgggttg cggagctcgt g
//
LOCUS       DASTZR010000080         6670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1049615, whole genome shotgun sequence.
ACCESSION   DASTZR010000080 DASTZR010000000
VERSION     DASTZR010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6670
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049615"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..1060
                     /locus_tag="VFV20_01800"
     CDS             47..1060
                     /locus_tag="VFV20_01800"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU5287111.1"
                     /translation="MEELNDTQRYFVDEHVEDYRDGLITRRELVRRVTLIAGSAAAAA
                     TILSACDLSPRGGAGGVPSPTPAGTSAGGGASPTAGLVAAGPYATPPAALSTDGITVK
                     PDDPRITVSKPDVRGTDGAALQAYMAKPNASGRVPGILVVHENRGQTEHIRDVVRRVA
                     TAGFVGLNIDLAARDGGAEKLTDQAAYNAALGKRSTADKLSDHKAALDFLKAQSSGTI
                     GVTGFCFGGGEVWNILAGGLEVKAAVPYYGPQPSNFTEISKTKAAVFAVYAELDTRIT
                     GTASQMEAELKKSGAPYQVTVYPGVNHAFHNDTGTAERYNAEQARKAWVATIEWFRKY
                     LTT"
     gene            1106..2134
                     /locus_tag="VFV20_01805"
     CDS             1106..2134
                     /locus_tag="VFV20_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287112.1"
                     /translation="MRIAIVALLVAAACGTTANAPGATSTNVVAPASPSPTQPAPTVT
                     AAPSPTPRANPTQGPGTYIGTALAYRVDLPAGWRRSACQSTRALAQPPGSETFTVATV
                     DEEGGGDVGGAQQDVVVVHAEDAAGQTPMQWLQSGRLGLSISTRFEATTVDGKEAARM
                     LDADNGRATAYAVAARGLMYVLSRGQREPTAASEPGATAIIRSFHVLTDAELSAARGT
                     IATPSPAPSRSAEDVVAALARGFAQKDTTVLASVADACLTHGVEQGGAGFASTSRVLG
                     DMQRSFASGFVATVRQGPLEDRTAFNATVRGTWQDPSQPQKNVKFMLMAVGNTWYWSG
                     WIDLQPVR"
     gene            complement(2139..3611)
                     /gene="tig"
                     /locus_tag="VFV20_01810"
     CDS             complement(2139..3611)
                     /gene="tig"
                     /locus_tag="VFV20_01810"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEU5287113.1"
                     /translation="MTDTPSTATPAERSFRHTLTREPGARAVLEVEVDAERVTRQADR
                     VFERHNQKARIPGFRPGKAPRAMYERQYGIEHLWTEAAEDLVDETYREILDLEDLDPI
                     DNPSVELTALEPGKPLKYKATVVVKPNVELGEYAAHGATVEPAPPTDEEIGKTIDSMR
                     EQHAQLKPVQRESRAGDIVTVDIDLTVDGKAMPPFARNAHVEAGRDSPTIKGLGDAFV
                     GLTVGTEKKVDLPFSDDIADEQVRGKTGAFAIRASQIAEKIVPDLDDEFAKTVGVASV
                     EELRKAVKNELAHAAFHESRDAAADKFMEHALSVSAVEVPELLVQDELDHLVADLKSR
                     VREQGITFEQFLLQARKTEDELRKEWREAAERRAKAMLVLEEIAKKEGVTVTSQELAA
                     QVAMTPLAQQDPQALRDPVLLASLARSIRNRKTVDKLIGLDSPDAESEAIKKAGGEAV
                     DFHGTGPAPKPEEPKIIVPTKTDATPEGREALRAMLEKKD"
     gene            3635..3991
                     /locus_tag="VFV20_01815"
     CDS             3635..3991
                     /locus_tag="VFV20_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287114.1"
                     /translation="MFFYEIHEGDEEVGAAVLLGHEQQFEPVDFFDLVKKVRTLVMDT
                     YEEDTLSEAIANELQRSHGFIHVTDDLLVASVNVDESDEETFLVTESEDTRTIFVQRE
                     ENGHTAPDTENGSRDN"
     gene            3994..4896
                     /locus_tag="VFV20_01820"
     CDS             3994..4896
                     /locus_tag="VFV20_01820"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5287115.1"
                     /translation="MAERIGFVGTGIMGAPMARNALKAGYQVTVTNRTLARAEPLRTD
                     GATVVGTPREVAQRSDIVVTMVVDSPDVEQVLFGPEGVASGAHDGLLAIDMSTISPAR
                     TREFAERAAQNAPAFRTLDAPVSGGEIGAIEARLSIMIGGDRTDVERAMPFFEALGKT
                     IVHVGDHGAGQACKLANQIAVAINNLGVAEALVFAASQSIDLEKVRQVIAGGAGSSWA
                     MQNYAPKMLAGDFRPGFMVDLQQKDLRLVLGDADAAHLALPGTSLAHHLYNALQAAGE
                     GRDGNHALLKVIERLSKTEARVRR"
     gene            5166..5254
                     /locus_tag="VFV20_01825"
     tRNA            5166..5254
                     /locus_tag="VFV20_01825"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5202..5204,aa:Leu,seq:tag)
     gene            5343..5762
                     /locus_tag="VFV20_01830"
     CDS             5343..5762
                     /locus_tag="VFV20_01830"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF013398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HEU5287116.1"
                     /translation="MTLQFAQAAFREAPPPDQIVAGPITFVAHCDHQGIPFFGRAYVG
                     YIPSGDRVADTTLRRMVQRAARRGEDALERELARMLGMWTRPLGIAVVMRTAHECAGP
                     QLLADGGDREITFWRGRYRSDRSLRSAFLARCIRDMQ"
     gene            5890..>6670
                     /locus_tag="VFV20_01835"
     CDS             5890..>6670
                     /locus_tag="VFV20_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5287117.1"
                     /translation="MRASVLVVDDDEGVRSVITTALEDAGYRCLVARDGREGVQIARA
                     KLPDLVILDIVMPELSGDEAQRMLRHDPRTRYIPVMFVTGQGKSRDKAARLLGGADDY
                     VTKPFAIEELVARVGAVLRRSAELRALNPLSGLPGNVAIANEIARRLEGDDGSACMYV
                     DLDHFKEFNDHYGFARGDEVIVRLSRLLLGIAARHDEVFVGHVGGDDFVLIVADAEAP
                     DVAREIVREFDAITPSLYDPADRARGGIVRVDRRGVEVHLPF"
BASE COUNT         1011 a         2315 c         2327 g         1017 t
ORIGIN      
        1 cccgccccga ctaaaccgtt tggctggtat cgacgtatag gtgggcatgg aagaactgaa
       61 cgacacccag cgctatttcg tggacgaaca cgtcgaggac taccgggacg gcctcatcac
      121 ccgccgcgag ctcgtgcgcc gcgtcacgtt gatcgcgggc agcgccgcgg cggccgcgac
      181 gatcctctca gcatgcgatc tgtcaccgcg cggcggcgcc ggcggcgtcc catcgcccac
      241 cccggccggc acgagcgccg gcggcggggc cagcccgacg gcggggctcg tcgcggccgg
      301 cccgtacgcc acgccacccg cagcgctctc gacagacgga atcaccgtca aacccgacga
      361 cccccggatc accgtgtcca agcccgacgt ccgcggaacg gacggtgcgg ctctccaggc
      421 gtacatggcg aagccgaacg cctccggtcg cgtccccggg atcctcgtcg tccatgagaa
      481 ccggggacag accgagcaca tacgcgacgt cgtccggcgc gtcgccacgg cgggattcgt
      541 cgggctgaac atcgacctcg cggcccgcga tggcggagcg gagaagctca cggaccaggc
      601 cgcgtacaac gcggcgctcg ggaagcgttc gaccgccgac aagctctccg accacaaggc
      661 ggccctcgac ttcctgaagg cgcagtcgag cggcacgatc ggcgtgaccg ggttctgctt
      721 cggcggcggc gaggtctgga acatcctggc cggcgggctc gaggtcaagg cggcggtccc
      781 ctattacgga ccgcagccgt cgaacttcac cgagatctcg aagacgaagg cggccgtgtt
      841 cgcggtgtac gcggagctgg acacacgcat caccgggacg gcgtcgcaga tggaggcgga
      901 gctcaagaag tcgggagctc cctaccaggt gacggtctat cccggggtga accacgcgtt
      961 ccacaacgac acgggtaccg cggagcgcta caacgccgag caggcgcgga aggcctgggt
     1021 cgcgacgatc gagtggttca ggaagtacct gacgacctga cgggctagcc gccgcgagcg
     1081 ggcggagcgc gtatcgtccg tcgcgatgcg gatcgcgatc gtcgcgctcc tcgtcgcagc
     1141 ggcgtgcgga acgacggcga acgcgcccgg cgcgacgagc acgaacgtcg tcgcgccggc
     1201 gtcgccgagt ccgacgcagc ccgcgccgac ggtgacggcc gcgccgtcac cgacgccgcg
     1261 tgccaatccg acccagggac cggggacata catcgggacg gcgctcgcgt atcgcgtcga
     1321 ccttcccgcc ggctggcgca ggtccgcgtg ccagtccacg cgcgcgctcg cccagccgcc
     1381 cggatccgag accttcacgg tggcgaccgt cgacgaggag ggcggcggtg atgtcggcgg
     1441 tgcgcagcag gacgtcgtcg tcgtgcacgc ggaggacgcg gcggggcaga cgcccatgca
     1501 gtggctccag tcggggcggc ttggcctctc catctccacg cggttcgaag cgacgacggt
     1561 cgatggcaag gaggccgcgc gcatgctcga cgccgacaac gggcgtgcga cggcctacgc
     1621 ggtcgcggcc cgcgggctca tgtacgtgct gtcgcgcggc cagcgtgagc cgacagcggc
     1681 gtccgagccg ggcgccaccg cgatcatccg ctccttccac gtcctcaccg acgccgagct
     1741 ctccgcggcg cgcggcacga tcgcgacgcc gtcgcccgcg ccgtcgcggt ccgcggagga
     1801 cgtcgtcgcc gcgctcgcgc gcggcttcgc gcagaaggac acgaccgtcc tcgcgagcgt
     1861 cgccgatgcg tgcctcacac acggtgtcga gcagggcggc gcgggcttcg cgtccacatc
     1921 gagggtcctc ggagatatgc agcggtcatt cgcgagcggc ttcgtcgcga cggtgcggca
     1981 gggcccgctc gaggatcgga ccgcctttaa cgcgacggtg cgcggcacgt ggcaggaccc
     2041 gagtcagccg cagaagaacg tgaagttcat gctgatggca gtcggcaata cctggtactg
     2101 gtccggatgg atcgacctgc agccggtgcg ctagggacct agtccttctt ctccagcatc
     2161 gcgcgcagcg cctcgcgccc ctcgggcgtc gcgtccgtct tcgtcgggac gatgatcttc
     2221 ggctcctccg gcttcggcgc cggaccggtg ccgtggaagt cgacggcctc gccgccagcc
     2281 ttcttgatcg cctccgactc cgcgtcgggc gagtcgagcc cgatgagctt gtcgaccgtc
     2341 ttgcggttgc ggatggagcg cgccagcgat gcgagcagga cgggatcgcg cagcgcctgc
     2401 gggtcctgct gcgcgagcgg cgtcatggcg acctgcgcgg cgagctcctg cgacgtgacg
     2461 gtgacgcctt ccttcttcgc gatctcctcg agcacgagca tcgccttcgc gcggcgctcg
     2521 gccgcctccc gccactcctt gcgcagctcg tcctccgtct tgcgcgcctg cagcaggaac
     2581 tgctcgaacg tgatcccctg ctcgcggacg cgcgacttca ggtccgcgac gagatggtcg
     2641 agctcgtcct gcacgagcag ctcgggcacc tcgacggccg ataccgagag cgcgtgctcc
     2701 atgaacttgt cggccgccgc gtcacgcgac tcgtggaagg cggcgtgcgc cagctcgttc
     2761 ttcacggcct tgcgcagctc ctcgacggac gcgacgccga cggtcttcgc gaattcgtcg
     2821 tcgagatcgg gcacgatctt ctcggcgatc tggctcgcac ggatggcgaa tgcgccggtc
     2881 ttgccgcgca cctgctcgtc cgcgatgtcg tccgagaagg gcaggtccac cttcttctcg
     2941 gtgccgacgg tgagcccgac gaacgcgtcg ccgagtccct tgatggtggg cgaatcgcga
     3001 cccgcctcga cgtgggcgtt gcgggcgaat ggcggcatgg cctttccgtc cacggtgagg
     3061 tcgatgtcca ccgtcacgat gtcgccggca cgcgattccc gctgcacggg cttcagctgc
     3121 gcgtgctgct cgcgcatcga gtcgatcgtc ttgccgatct cctcgtcggt cggcggcgcg
     3181 ggctcgaccg tcgcgccgtg cgcggcgtac tcgccgagct cgacgttcgg cttcaccacg
     3241 acggtcgcct tgtacttcag cggcttgccg ggctcgagcg cggtcagctc gaccgacggg
     3301 ttgtcgatgg gatcgaggtc ctcgaggtcg aggatctccc ggtacgtctc gtcgacgagg
     3361 tcttcggccg cctccgtcca gaggtgctcg atgccgtact ggcgctcgta catcgcgcgc
     3421 ggggccttgc ccggacggaa accggggatg cgcgccttct ggttgtgccg ctcgaacacg
     3481 cggtcggcct ggcgcgtcac gcgctcggca tcgacctcca cctcgaggac ggcccgcgcc
     3541 cccggctcac gcgtgagcgt atggcggaag gagcgctcgg cgggcgtggc ggtcgagggg
     3601 gtgtccgtca tggagttgga gatagtctag cccgatgttc ttctacgaga tccacgaggg
     3661 cgacgaggag gtcggcgccg cggtcctcct gggtcacgag cagcagttcg agccggtgga
     3721 cttcttcgac ctggtgaaga aggtgcgcac gctcgtcatg gacacctacg aggaggacac
     3781 gctctccgag gcaatcgcga acgagctcca gcgcagccac ggcttcatcc acgtgaccga
     3841 cgatctgctc gtcgcctcgg tgaacgtcga cgagtcggac gaggagacgt tcctcgtcac
     3901 cgagagcgag gacacacgca cgatcttcgt ccagcgcgag gagaacgggc acaccgctcc
     3961 cgacaccgag aacggatcgc gcgacaacta gccgtggcgg agcggatcgg ctttgtcggc
     4021 accgggatca tgggcgcacc catggcgcgc aacgcgctga aggccggcta tcaggtcacg
     4081 gtcacgaacc ggacgctggc gcgcgccgag cccctccgga cggacggcgc gaccgtcgtc
     4141 ggcacgccgc gcgaggtcgc gcagcgctcg gacatcgtcg tcacgatggt ggtggactcg
     4201 cccgacgtcg agcaggtcct gttcggtccg gagggcgtcg cgagcggcgc gcatgacgga
     4261 ctgctcgcga tcgacatgag cacgatctcg cccgcgcgga cgagagagtt cgcggagcgc
     4321 gccgcccaga acgcgcccgc cttcaggacg ctggacgcgc cggtgtccgg cggcgagatc
     4381 ggcgccatcg aggcgaggct ctcgatcatg atcggcggcg accgaacgga cgtggagcgc
     4441 gcgatgccgt tcttcgaggc gctcgggaag acgatcgtgc acgtcggcga tcacggagcg
     4501 ggacaggcgt gcaagctcgc caaccagatc gccgtcgcga tcaacaacct cggcgtggct
     4561 gaggcgctcg tgttcgcggc gtcgcagagc atcgacctcg aaaaggtgcg tcaggtcatc
     4621 gcgggcggcg cggggtcgtc gtgggcgatg cagaactacg cgccgaagat gctcgccggc
     4681 gatttccgcc ccggcttcat ggtggatctc cagcagaagg acctgcgcct ggtgctcggc
     4741 gacgccgacg ccgcgcatct cgcgctcccc gggacgtccc tcgcgcacca cctgtacaac
     4801 gcgctgcagg ccgccggcga gggccgcgac gggaaccacg ccctgctgaa ggtcatcgag
     4861 cgcctctcga agaccgaggc acgcgtacgg cggtagacgc gttcgacacg gcgttgccgc
     4921 ttctcgcgcg catgtcggac actcagatcg tctgggacta cggggctgga tctggatcaa
     4981 cctcaggcga gggaacggca tcggctgcct cgcatatgcg aaacacgaca gcctcaagct
     5041 cctgccgtgg ctctatcccg atcccgaggt gccctgcctg ctgcggaaac gtgccatctg
     5101 ggatgactac gtggcgaggc atccgagtga catcgggaag cccccctcta acatctatgc
     5161 ctagagcccg ggtggcgaaa cctggctata cgcgtcggcc ttaggagccg atggggctaa
     5221 tcaccccgtg tgggttcgaa tcccaccccg ggcacccgca cggatgatgg tccgaatccc
     5281 tcctcgccca ccggtcgagc gggggtttcg cggggcacag cgagtgcatt gggcggaggc
     5341 tcgtgacgct gcagttcgcc caagccgcct tccgtgaggc gccgccaccc gaccagatcg
     5401 tggccggccc catcacgttc gtggcgcact gcgatcacca gggcatcccg ttcttcgggc
     5461 gcgcgtacgt gggatacatc ccctccggcg accgcgtggc cgacacgacc ctgcggcgca
     5521 tggtgcagcg cgccgcccgg cgcggtgagg acgcgctcga gcgcgagctc gcgcggatgc
     5581 tcggcatgtg gaccagaccg ctcggcatcg ccgtcgtcat gcggacggcc cacgagtgcg
     5641 cgggccccca gctgctcgcc gacggcggcg atcgggagat cacgttctgg cgcggccggt
     5701 accgcagcga tcgttcgctc cgctcggcct tcctcgcgcg ctgtatccgc gacatgcagt
     5761 gagcgcggcg tcgccgcacc gggcgcaccg ccctgttcgc ggcgggaccg cggcggcctg
     5821 gcaaccgacg gtactgctac gcctttccca tatggacggg accacggcgg ccctcatgct
     5881 tgctctgaca tgagagcctc ggtgctcgtc gtcgacgacg atgagggcgt gcgcagcgtg
     5941 atcacgaccg ccctcgagga tgccgggtat cgctgcctgg tggcgcgcga cgggcgcgag
     6001 ggcgtccaga tcgcgcgcgc gaagctgccc gatctcgtca tcctcgacat cgtcatgccc
     6061 gaactctcag gtgatgaagc gcagcgcatg ctgcgccacg atccacggac gcgctacatc
     6121 ccggtgatgt tcgtcaccgg tcagggcaag agccgggaca aggcggcgcg actgctgggc
     6181 ggagcggacg actacgtgac gaagcccttc gcgatcgagg agctcgtcgc gcgcgtcggg
     6241 gccgtcctgc gccggtcggc ggagctgcgc gcgctgaatc cgctctcggg tctcccgggc
     6301 aacgtcgcga tcgcgaacga gatcgcgcga cgcctcgagg gcgacgacgg atcggcgtgc
     6361 atgtacgttg acctggacca cttcaaggag ttcaacgacc actatggctt cgcgcgcggc
     6421 gacgaggtga tcgtccgcct gtcgcggttg ctgctcggca tcgcagcccg tcacgacgag
     6481 gtcttcgtcg gtcacgtggg aggcgacgac ttcgtcctga tcgtcgcgga cgcggaggca
     6541 cccgacgtgg cgcgtgagat cgtgcgcgag ttcgacgcga tcacgccatc gctgtacgac
     6601 cctgccgacc gcgcgcgcgg cggcatcgtg cgcgtggatc gccgcggggt cgaggtccac
     6661 cttcccttcg
//
LOCUS       DASTZR010000081         2151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_292296, whole genome shotgun sequence.
ACCESSION   DASTZR010000081 DASTZR010000000
VERSION     DASTZR010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292296"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VFV20_01840"
     CDS             <1..276
                     /locus_tag="VFV20_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5287118.1"
                     /translation="MEEADELCDRVAFLSQGRIVALDTPRELKLKQGERRAVVLLKDR
                     SEQSLRLDDPADAARLDEWMRRDLVLTIHSQEGTLEDVFIALSGRAL"
     gene            273..1367
                     /locus_tag="VFV20_01845"
     CDS             273..1367
                     /locus_tag="VFV20_01845"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5287119.1"
                     /translation="MIRPRIVATLFRKDLVDALRESRVLISLLTPILLAVLYNLMFPD
                     EKVFAAKVAYAGPQDSAIVELLRTRAETGQAVDLTLRHVSSADEARRLVVTKDVDVAF
                     VLPDGVDEAIRSGATPTIGVIQPATITSAASWVESNITATARTIAGQKPPALVMPELV
                     KVGSADQGVMGQMGPRRYFVLATVVMMLGMIALLAVPIMLTEEAEKKTLDALLLVGSY
                     LEVIAAKALVGLTYVALSVIVMLGLTRLTPGDMATFVAGTGLLALALIAIGLLVGGLF
                     RSAQQVYSWSSVMLIPVVGPAFAVGLPVPDALDLVFKALPTSQGMRIMANGLAGKAVF
                     ADAWVSVAVTAVWAVAAFALLAWHLSRRES"
     gene            complement(1399..>2151)
                     /locus_tag="VFV20_01850"
     CDS             complement(1399..>2151)
                     /locus_tag="VFV20_01850"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5287120.1"
                     /translation="LGDPVLTPRLGLSVVRRAGLVLVSSVKLDAPVFNHTSGYGTFAA
                     PTQRGVDAVLRHDAAIGRRARIEVAIPGVSRSDRALLERSGFRDSATLFQCHIRTTAR
                     PPRARDVPGLLAERATARSAGTYARLATAGFGGRHSVIADVFERGWTRQIKGDRRVAA
                     FIGRVRGRPAATGVLVMRPDIAGLYSGSVLPRYRGRGLQNAMIAARLRYGWDRGLRTF
                     YSWSDPDSASARNLRDEGFRTRYEVHMYEREP"
BASE COUNT          302 a          745 c          786 g          318 t
ORIGIN      
        1 atggaggagg ccgacgagct ctgcgatcgc gtggccttcc tgagccaggg ccggatcgtc
       61 gcgctcgaca cgccgcgcga gctgaagctg aagcagggcg agcgccgcgc ggtcgtgctg
      121 ctgaaggacc ggagcgagca gagcctgcga ctcgacgatc cggcggatgc ggcgcggctc
      181 gacgaatgga tgcggcgcga cctcgtcctc acgatccact cgcaggaggg cacgctcgag
      241 gacgtgttca tcgcgctgtc gggccgggcg ctgtgatccg gccgcggatc gtcgcgacgc
      301 tcttccggaa ggatctggtg gacgcgctgc gcgaatcgcg cgtgctcatc tcgctgctga
      361 cgcccatcct tctcgcggtc ctctacaacc tgatgttccc cgacgagaag gtcttcgccg
      421 ccaaggtcgc atatgccggc ccgcaggaca gcgcgatcgt cgagctgctg cggacgcggg
      481 cggagacggg acaggcggtg gacctgacgc tccgccacgt gtccagcgcg gacgaggcgc
      541 ggcgcctcgt ggtcacgaag gacgtcgacg tggccttcgt gctgcccgac ggggtggacg
      601 aggcgatcag gagcggcgcg acgccgacga tcggcgtcat ccagcccgcc acgatcacga
      661 gcgccgcgag ctgggtggag tcgaacatca ccgcgaccgc gcgcaccatc gccggacaga
      721 agccgcccgc gctcgtcatg ccggagctcg tgaaggtcgg atccgcggat cagggcgtca
      781 tgggccagat gggaccgcgc cgctacttcg tactggcgac ggtggtgatg atgctgggca
      841 tgatcgccct cctcgcggtg ccgatcatgc tcaccgagga ggccgagaag aagacgctcg
      901 acgcgctcct gctcgtcggt tcgtatctcg aggtgatcgc cgcgaaggcg ctcgtcggcc
      961 tcacgtacgt ggccctctcg gtcatcgtga tgctcgggct cacacgcctg acgccgggcg
     1021 acatggcgac gttcgtcgcc ggcacgggac tcctcgcgct cgcgctcatc gccatcggcc
     1081 tgctcgtcgg cggcctcttc cgcagcgcac agcaggtgta ctcgtggtcg agcgtcatgc
     1141 tcatcccggt cgtgggaccg gcgttcgccg tcgggcttcc cgtccccgac gcgctcgatc
     1201 tcgtcttcaa ggcgctgccc acgagccagg gcatgcgcat catggcgaac gggctcgccg
     1261 gaaaggcggt cttcgccgac gcgtgggtct cggtcgccgt caccgcggtc tgggcggtcg
     1321 ccgcgttcgc gctccttgcc tggcatctct cccgtcgcga gtcctagcgg gcgggcggac
     1381 gggccggtca cgcgagcgct acggctcgcg ctcgtacatg tgcacctcgt agcgggtgcg
     1441 aaagccctcg tcgcggagat tgcgcgccga ggcgctgtcg ggatccgacc acgaatagaa
     1501 ggtgcggagg ccgcggtccc acccatagcg cagccgcgcg gcgatcatcg cgttctgcag
     1561 cccgcgaccg cggtaccgcg gcagcacgct gcccgagtac agcccggcga tgtccggccg
     1621 catgaccagc acgcccgtgg cggccggcct cccgcggacg cggccgatga acgcggcgac
     1681 gcggcggtct cccttgatct ggcgggtcca gccgcgctcg aagacgtcgg cgatgaccga
     1741 gtgccggccg ccgaaacccg cggtcgcgag cctcgcgtag gtgccggccg aacgcgccgt
     1801 cgcgcgctcc gccaggaggc cgggcacgtc gcgcgcgcgt ggtggcctcg ctgtcgtgcg
     1861 gatgtggcat tggaagaggg tcgcgctgtc gcgaaagccg ctgcgctcga ggagcgctcg
     1921 atcgctgcgc gacacccccg ggatcgccac ctcgatgcgc gcgcgccggc cgatggccgc
     1981 gtcgtggcgg agcacggcgt cgacgccgcg ctgcgtgggc gctgcgaagg tgccgtaccc
     2041 gctcgtgtgg ttgaacacgg gagcgtcgag cttgacggat gacacgagca cgagcccggc
     2101 gcgccggacg acgctgaggc cgaggcgcgg cgtgagcacg ggatcgccga g
//
LOCUS       DASTZR010000082         4141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1023544, whole genome shotgun sequence.
ACCESSION   DASTZR010000082 DASTZR010000000
VERSION     DASTZR010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023544"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VFV20_01855"
     CDS             complement(<1..578)
                     /locus_tag="VFV20_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5287121.1"
                     /translation="MARLPKPRSDLGGFQAYRTQQQKADVRLQANEWADPNPAGKWLT
                     PDELDAVLLNRYPSAAVDLREALARRYGVTPDQLVIGNGSNEVLLNTFLVFGGHGRRT
                     LLFQPTYSMHGRLTLIAGGTSVDEVVGLPYALTRERAMAAMERVRPEIVVFTTPNNPT
                     GNEIPFDAILSVANAFPESLVLVDEAYSDFART"
     gene            599..1081
                     /locus_tag="VFV20_01860"
     CDS             599..1081
                     /locus_tag="VFV20_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287122.1"
                     /translation="MRPPSRGVRCAHAQPTIGERAAFVTAVLGVALGALFLFAPIHGY
                     CMTSVSATPVPPGATPGPVMTSSFCGSEALWQRQPLFPMPFFAVLVWSLAPLVVYVGV
                     RLRAGGERNTGTVLAIAGFLLECTVLISFGAAPFFAPFVLLPLVITMALAFRVPSRGS
                     "
     gene            1078..1569
                     /locus_tag="VFV20_01865"
     CDS             1078..1569
                     /locus_tag="VFV20_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287123.1"
                     /translation="MTLGNASAVAGERIRGSRAAHLATLLCVLAAAAAVTYLGYFLFG
                     PTYNGCELGPVSPTAAPGAPVCRTMGWLEMQFGRGYPGPIDLRPLAFLGAWTAAPFVA
                     LVGTKLRSRGVGTSLVALALLVDASSIISMGGGFVYAILCGPLLLVALLAMLVAFTQS
                     RMG"
     gene            1586..1657
                     /locus_tag="VFV20_01870"
     tRNA            1586..1657
                     /locus_tag="VFV20_01870"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1618..1620,aa:Gln,seq:ctg)
     gene            complement(1677..2819)
                     /locus_tag="VFV20_01875"
     CDS             complement(1677..2819)
                     /locus_tag="VFV20_01875"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HEU5287124.1"
                     /translation="MRPTTDSAIVFNAERAAPAKLRLPHALAFHRALPAYRVTPLAEL
                     PDVARDLGVASVVVKDEELRLGLPSFKLLGSSWAVHERIRRRVGRPDGAVLSLDDLRE
                     HARAWDLTLCAASDGNYGRSIAALAELLGCRAVVLLPADTAAQRIEDVRAHGARVELV
                     DGSYEETVARARRSALAGGHWYCPDTALADADPSELEFVRDVVAGYTTLFVELVDQLG
                     RAPDVLFVQAGVGGLAAGAVLAFDELAQLARVVTVEPAGSDCVRASIAAGAPRTVPDR
                     PTIMAGLRCQSVSAVAWPMLHARVDAALAISDADAAAAMRLLAAHGIVAGESGAAGLA
                     GARVALRREDVRTRLGIGAGSIVAVVNTEGATDRASYERITEAGSG"
     gene            complement(2858..3817)
                     /locus_tag="VFV20_01880"
     CDS             complement(2858..3817)
                     /locus_tag="VFV20_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287125.1"
                     /translation="MNVVEWLLDADPAIRWQVMRDLTDASPAEVAAERVRVATEGWGA
                     QLLAWQGPDGRWDGGTYRPGWVDESKPFFDAWTATHFSLWLMRDLGLVPDGPEARRAV
                     SLVRDNVRWEANGALYFEGETEPCINGIALASGAYFGENVDGIAERLVSEALGDGGWN
                     CWAAYGAKVSSFHSTLCVLEGLLEWERAGGSSNAVAAARRRGEEYLLERKLLRRRSTG
                     EIVDPRFTMFSYPTWWYYDVLRALDYFRATGSAPDDRLAEAIALVASKRGEDGRWPLE
                     NTHQGPTHFAMDDPGEGFPSRWNTLRALRVLRWADPASTRPGW"
     gene            complement(3936..>4141)
                     /locus_tag="VFV20_01885"
     CDS             complement(3936..>4141)
                     /locus_tag="VFV20_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003195677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEU5287126.1"
                     /translation="ATGPMLRADPTERGFKDTVKANPGTFTIIRAKFDLPSGVRAPQR
                     YVHHCHIVEHEDNDMMRPYDVIA"
BASE COUNT          589 a         1480 c         1414 g          658 t
ORIGIN      
        1 gtccgcgcga agtcgctgta cgcctcgtcg acgagcacga gcgattcggg gaatgcgttc
       61 gcgaccgaga ggatcgcgtc gaaggggatc tcgttcccgg tggggttgtt cggcgtcgtg
      121 aagaccacga tctcgggccg cacgcgctcc atcgccgcca tcgcgcgctc gcgcgtgagc
      181 gcgtacggaa ggccgacgac ctcgtcgacc gaggtgccgc cggcgatgag cgtgagccgc
      241 ccgtgcatcg agtacgtggg ctggaacagg agcgtcctcc ggccgtggcc gccgaagacg
      301 aggaaggtgt tgaggaggac ctcgttgctg ccgttcccga tgacgagctg gtctggcgtg
      361 acgccgtagc ggcgggcgag cgcctcgcgc agatcgaccg cggcgctcgg atagcgattg
      421 agcaggacgg cgtcgagctc gtcgggcgtg agccactttc ccgccggatt cgggtcggcc
      481 cactcgttcg cctggagccg gacatccgcc ttttgctgct gcgtgcggta cgcctggaac
      541 ccgccgaggt cggatcgggg cttcgggagc cttgccatca cgcatagcgt gccatgactt
      601 gcgcccgccg tcccgcggcg tacggtgcgc gcatgcgcag cccaccatcg gcgagcgcgc
      661 ggcattcgtc acggccgtcc tcggcgtcgc gctcggcgcg ctgttcctct tcgcgccgat
      721 ccacggctac tgcatgacga gcgtgagcgc gacaccggtg ccgcccggcg cgacccccgg
      781 gcccgtgatg accagctcgt tctgcggcag cgaagccctg tggcagcgac aaccgctctt
      841 cccgatgccg ttcttcgcag tgctcgtgtg gtccctcgcg ccgctcgtcg tctacgtcgg
      901 tgtgcgtctg cgcgccggcg gcgagcgcaa caccgggacc gtgctggcga tcgcgggttt
      961 cctcctcgaa tgcaccgtgc tcatcagctt cggcgcggca ccgttcttcg cgccattcgt
     1021 gttgctgccg ctcgtcatca cgatggcgct cgcgttcagg gtgccctcga ggggatcatg
     1081 acgctcggca acgcttcggc ggtggccggt gagcgcattc gcgggtcgcg tgccgcacat
     1141 ctcgcgacgc tcctctgcgt gctcgcggcc gccgcggcgg tcacgtatct cgggtacttc
     1201 ctcttcggac ccacctacaa cggctgcgag ctcggtccgg tgtctccgac ggccgcgccc
     1261 ggagcgccgg tctgccgcac gatgggctgg ctcgagatgc aattcggccg cggctatccc
     1321 ggtccgattg acctgcgtcc gctcgcgttc ctcggcgcgt ggaccgcggc gccattcgtg
     1381 gccctcgtgg gcacgaagct gcgttcacgc ggcgtcggca cctcgctcgt cgcgctcgcg
     1441 ctcctcgtcg atgcgtcgag catcatcagc atgggcggcg ggttcgtcta cgcgatcctc
     1501 tgcggtccat tgctgctcgt cgcgctgctc gcgatgctgg tggcgttcac gcagtcgcgg
     1561 atgggataat cgaagtctca ctcattgggg agtggccaag cggtaaggca gctgactctg
     1621 gatcagccaa tcggaggttc gaatcctccc tccccagcag gcattctccc atcggatcag
     1681 ccagagcctg cctctgtgat gcgctcgtag gacgcgcgat ccgtcgcgcc ttctgtgttc
     1741 accacagcga cgatcgatcc ggcgccgatc ccgagacgcg tgcggacgtc ttcgcggcgc
     1801 agtgcgaccc gcgcgccggc gagacccgcg gcgcccgact cacccgcgac gatgccgtgc
     1861 gcggctagga gccgcatcgc cgcggcggcg tccgcatcgc tgatcgcgag cgcggcgtcg
     1921 acccgcgcat gcagcatcgg ccacgcgacg gcggacacgc tctggcagcg gagaccggcc
     1981 atgatcgtcg gccggtccgg aaccgtgcgt ggtgcgccgg cggcgatgga cgcgcgcacg
     2041 cagtccgatc ccgctggttc gaccgtcacg acgcgcgcaa gctgcgcgag ctcgtcgaac
     2101 gcgagcaccg cgcccgccgc gaggccgccc actcccgcct gcacgaagag cacgtccggc
     2161 gcgcgcccga gctggtcgac gagctcgacg aagagcgtcg tgtatcccgc gacgacgtcg
     2221 cgcacgaatt cgagctcgct cggatcggca tccgcgagcg cggtgtccgg gcagtaccag
     2281 tgaccgccgg ccaatgcgga gcgtcgcgca cgcgcgacgg tctcctcgta gctcccgtcg
     2341 acgagctcga cgcgcgcacc gtgcgcgcgc acgtcctcga tgcgctgcgc cgcggtgtcc
     2401 gcggggagga gcacgaccgc gcggcacccg aggagctccg cgagcgcggc gatcgagcgt
     2461 ccgtagttgc cgtcgcttgc tgcgcagagc gtcagatccc aggcgcgggc gtgctcgcgc
     2521 agatcgtcga gcgagaggac cgcgccgtcc gggcggccga cacggcggcg gatgcgctcg
     2581 tgcacggccc atgagctgcc cagcagcttg aacgacggca gcccgagccg aagctcctcg
     2641 tccttgacga cgacggacgc gacgccgagg tcgcgcgcga cgtccggcag ctcggcgagc
     2701 ggcgtgacgc gatacgcggg aagcgctcga tggaacgcga gcgcgtgcgg cagccggagc
     2761 ttcgcgggtg ccgcgcgctc cgcgttgaag acgatcgcgg aatccgtcgt cggacgaatc
     2821 taatggaggc tccgcgccct cgttcccccc gaggggccta ccagcctggg cgggtgcttg
     2881 ccggatccgc ccaccggagg acccgcagcg cgcgcagcgt gttccatcgg ctcgggaagc
     2941 cctcgccagg atcgtccatc gcgaaatgcg tcggcccctg gtgcgtgttc tcgagcggcc
     3001 acctgccatc ctctccgcgt ttgctcgcga cgagggcgat cgcctccgcg agccgatcgt
     3061 ccggtgccga cccggtggcc cggaagtagt cgagtgcgcg cagcacgtcg tagtaccacc
     3121 acgtggggta cgagaacatc gtgaaccgcg ggtccacgat ctcaccggtc gaacgacgac
     3181 gcagcagctt tcgctcgagg aggtactcct cgcccctgcg gcgcgccgcg gcgacggcgt
     3241 tcgaggatcc accggcgcgc tcccattcga gcagcccctc gagcacgcag agcgtgctgt
     3301 ggaacgaaga gaccttcgcg ccgtacgcgg cccagcagtt ccagccacca tcaccgagcg
     3361 cctcgctcac gagacgttcg gcgatgccgt cgacgttctc gccgaagtac gcgccgctcg
     3421 cgagcgcgat cccattgatg cacggctccg tctcgccctc gaagtacagg gcgccgttcg
     3481 cctcccaccg cacgttgtcg cgcacgaggg agaccgcgcg gcgtgcctcg ggaccgtccg
     3541 ggaccagacc gagatcgcgc atgagccaca gcgagaagtg cgtcgccgtc cacgcatcga
     3601 agaacggctt cgactcgtcg acccaaccgg gccggtaggt accgccgtcc cagcgcccat
     3661 cgggaccctg ccatgcgagc agctgcgcac cccagccctc ggtggcgacg cgaacgcgtt
     3721 cagccgcgac ctcggcgggc gacgcatccg tgagatcgcg catcacctgc cagcggatgg
     3781 ccggatcggc gtccagcagc cactcgacga cgttcattgc caagtgacga tggctcacac
     3841 gcgatcgcgt cctacgacct gctcacccat ccttcgtagc agataccgga cgggcctgcg
     3901 cgtgtgtgac ccggcctggc cccgcttcaa gcgcctcagg cgatcacgtc gtacggccgc
     3961 atcatgtcgt tgtcctcgtg ctcgacgata tggcagtggt gcacataccg ctgcggcgcg
     4021 cgtacgccgg acggtaggtc gaacttcgcg cggatgatcg tgaaggtacc ggggttcgcc
     4081 ttgaccgtgt ccttgaagcc gcgctccgtc ggatccgcgc gaagcatcgg tccggtggcg
     4141 a
//
LOCUS       DASTZR010000083         2577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1075634, whole genome shotgun sequence.
ACCESSION   DASTZR010000083 DASTZR010000000
VERSION     DASTZR010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075634"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /gene="cgtA"
                     /locus_tag="VFV20_01890"
     CDS             <1..903
                     /gene="cgtA"
                     /locus_tag="VFV20_01890"
                     /inference="COORDINATES: protein motif:HMM:NF014474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HEU5287127.1"
                     /translation="RGNARFATSTRRAPRIALDGERGEERELELELRLIADVGLAGLP
                     NAGKSSLLAALTRARPKIAAYPFTTLTPNLGVARLDERELVIADIPGLIEGASTGAGL
                     GEEFLRHIERTRLVVHVVDASLPDPLADIATIDAELDAYGHGLRELPELVALNKIDLA
                     EARDRVPSFVEALTAQGRESVAVSAASGEGVDRLAKRLFSLVPPRAVAAAAEPAERRI
                     VFKGGPADVRVEREGEAYRVRGERIERLATGIDWDSGEASAYFHRMLQRHGVERRLRE
                     LGVKEGDTVRVGKLELEWRDAAPR"
     gene            900..1457
                     /gene="nadD"
                     /locus_tag="VFV20_01895"
     CDS             900..1457
                     /gene="nadD"
                     /locus_tag="VFV20_01895"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006694324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEU5287128.1"
                     /translation="MTRVGVFGGTFDPVHVGHLAIALAALESARLDRIVFVPARRSPL
                     KDAPPAASAADRLAMLEAATKDEPRFSVSRVELEREGPSYTVDTLDALSAEGELFLIL
                     GSDALAELDRWRSPQRIRSLATILVARRPGAPEPAAGVATAFDAPCLDISSRELRARG
                     ARGLSLRYLVPDEVWRHIERRALYR"
     gene            1454..1822
                     /gene="rsfS"
                     /locus_tag="VFV20_01900"
     CDS             1454..1822
                     /gene="rsfS"
                     /locus_tag="VFV20_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU5287129.1"
                     /translation="MSETARRDIAPRAMADLVVDAASDKKAEDILVLNVSEVTTIADL
                     FVICSGRGERQVQAIADAIVEKAKAAGRQPVGVEGYDAGRWVLIDLGDVVTHVFVPDE
                     RQLYRLERLWGDAPVVLRIA"
     gene            complement(1857..2195)
                     /locus_tag="VFV20_01905"
     CDS             complement(1857..2195)
                     /locus_tag="VFV20_01905"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU5287130.1"
                     /translation="MRVLIMAKGERSEPGASPTPEAMAEFMKFNDELIKAGVVVASGR
                     LTPSAQGKRVRFDGKKRVVIDGPFAEAKEVVGGYWLWQVGSLEEAIEWLKRAPYEGGT
                     FEVRPVAEEM"
     gene            complement(2310..2382)
                     /locus_tag="VFV20_01910"
     tRNA            complement(2310..2382)
                     /locus_tag="VFV20_01910"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2347..2349),aa:Ala,seq:cgc)
BASE COUNT          380 a          948 c          874 g          375 t
ORIGIN      
        1 cgaggcaacg cgcgcttcgc cacgagcacg cgacgcgcgc cgcgcatcgc gctcgacggt
       61 gagaggggcg aggagcgtga gctggagctg gagctgcgac tcatcgccga cgtcggtctc
      121 gcgggattgc cgaacgccgg caagtcgagc ctgctcgcgg cgctgacgcg cgcgcggccc
      181 aagatcgccg cgtatccgtt cacgacgctc acgccgaacc tcggggtcgc ccgactcgac
      241 gagcgcgagc tcgtcatcgc ggacatcccc ggtctcatcg agggtgccag caccggcgcg
      301 gggctcggcg aggagttcct ccgtcacatc gagcggacgc gcctggtcgt ccacgtcgtc
      361 gatgcctcgc tgcccgatcc gctcgcggac atcgcgacca tcgacgcgga gctcgacgcc
      421 tacggtcacg gtctgcgcga gctccccgag ctcgtggcac tgaacaagat cgacctggcc
      481 gaggcgcgcg accgcgtccc ctcgttcgtc gaagcgctca ccgcgcaggg ccgagagtcc
      541 gtcgccgtct cggccgcgag cggcgagggc gtcgaccgcc tggcgaagcg cctcttctcg
      601 ctcgtcccgc cacgcgcggt cgcggccgcg gcggaacccg ctgagcgcag gatcgtgttc
      661 aagggcggcc cggcggacgt ccgcgtcgag cgcgagggcg aggcatatcg cgtgcgcggc
      721 gagcgcatcg agcgcctcgc caccggcatc gattgggaca gcggtgaggc ctccgcctac
      781 ttccaccgca tgctgcagcg gcacggcgtc gagcgccgtc tgcgcgagct cggcgtgaag
      841 gagggcgaca cggtgcgcgt cggcaagctc gagctcgaat ggcgggacgc cgcaccccga
      901 tgactcgcgt cggcgtcttc ggcggcacgt tcgatccggt ccatgtcgga cacctcgcga
      961 tcgccctcgc cgcactggag agcgcgcggc tcgaccgcat cgtcttcgtg ccggcgcgcc
     1021 ggtcaccatt gaaggacgcc ccgccggccg cgagcgccgc ggaccgcctc gcgatgctcg
     1081 aggccgctac gaaggacgag ccgcgcttct ccgtgtcgcg cgtcgagctc gagcgcgaag
     1141 gtccctcgta caccgtggac acgctggacg cgctttcggc ggagggcgag ctgttcctca
     1201 tcctcgggag cgacgcgctc gcggagctgg accgctggcg ctcgccgcag cgcatccgct
     1261 ccctcgcgac gatcctcgtc gcgcggcgcc ccggcgcgcc ggagcccgcc gcgggcgtgg
     1321 cgacggcgtt cgacgcgccc tgtctcgaca tcagctcgcg cgagctccgc gcacgtggcg
     1381 cccgcgggct ctcgctgcgc taccttgttc ccgacgaggt gtggcggcac atcgaacgca
     1441 gggcgttgta ccgatgagcg agaccgcgcg ccgcgacatc gcaccgcgag cgatggccga
     1501 cctggtcgtc gacgcggcga gcgacaagaa ggccgaggac atcctggtcc tcaacgtctc
     1561 cgaggtcacg accatcgccg atctcttcgt catctgctcc ggccgcggcg agcggcaggt
     1621 gcaggccatc gcggacgcga tcgtcgagaa ggcgaaggcg gccggtcggc agcccgtcgg
     1681 cgtcgagggc tacgacgccg gtcgctgggt gctcatcgat ctcggcgacg tggtcacgca
     1741 cgtcttcgtc cccgacgagc ggcagctcta ccggctcgaa cgcctctggg gcgacgcacc
     1801 ggtggtgctg cgcatcgcgt aaggcgcgtc gcggccagcg cagctccccg cgatccttac
     1861 atctcctcgg cgaccggccg cacctcgaag gtcccgccct cgtacggcgc gcgcttgagc
     1921 cattcgatcg cctcctcgag cgagcccacc tgccacagcc agtagccgcc cacgacctcc
     1981 ttggcctcag cgaatggccc gtcgatcacg acgcgtttct tgccgtcgaa acgaacgcgc
     2041 ttgccctggg cgctcggggt gagccgaccg gacgcgacca cgacgccggc cttgatcagc
     2101 tcgtcgttga acttcatgaa ctccgccatc gcctcgggcg tcggcgaggc cccgggctcg
     2161 ctgcgctcgc cctttgccat gatcagcaca cgcatgtgtg tctcctctgc caaagatggc
     2221 tcctcaaagc ctcaccctta cgtcgaacgg cagccggcgt tttcgacaga ccgcaccatg
     2281 gagaagagca ccgcaggcaa agaattcggt ggagctgagg ggattcgaac ccctgacctc
     2341 ttgagtgcga ttcaagcgct ctcccagctg agctacagcc cctggggacc ggaccgacgc
     2401 gccgtcaccg gtaccgaggc gacggtcgga actgctcaat agtatcgggc gccgccgggc
     2461 cgcgccaggc tccgccgatg tgtttcgcgg cacgacggcg cgttcaagga gtccgcgaca
     2521 taatcgacgc gatgggaccc ttctccagcg gcggcgaccc cgagcgcgcc gcgaaca
//
LOCUS       DASTZR010000084         2897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1154155, whole genome shotgun sequence.
ACCESSION   DASTZR010000084 DASTZR010000000
VERSION     DASTZR010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154155"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VFV20_01915"
     CDS             <1..279
                     /locus_tag="VFV20_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEU5287131.1"
                     /translation="VLLFSSRGRLSREAAAAVGQLHERGARVYAVTSDARVAERADDA
                     VLLGSPVGEALAPIALAVVAQHLARAVALRHGRDPERPAGLSKVTRTL"
     gene            complement(655..1578)
                     /locus_tag="VFV20_01920"
     CDS             complement(655..1578)
                     /locus_tag="VFV20_01920"
                     /inference="COORDINATES: protein motif:HMM:NF014675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HEU5287132.1"
                     /translation="MKKALWVVRDKITTPDAIDALLAAAAARGIVDLVVQVRGRGDAY
                     YSPRLEPRAEGLAEGFDPLAHLIERGRSTGTRVHAWANVYFVWSHPERRLPRDEAHVV
                     NAHPDWLLRPDGVSALDPAGGTDWEGIYTDPANTAARRHTITVFADIATRYDVAGIHY
                     DYVRYPQVTYAGGPEDHRHVTDLVRESAARLRGARPDLVISAAVFPDPAIARDRVLQR
                     WPDWAADGVVDLLCPMAYRRDSTEVARLLGLARAAAPRTELWGGLMGYAGEPALLRTQ
                     VRAAKDAGCEGAILFQYDPAEPDLCDAFAAA"
     gene            complement(1575..2339)
                     /locus_tag="VFV20_01925"
     CDS             complement(1575..2339)
                     /locus_tag="VFV20_01925"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEU5287133.1"
                     /translation="MRLIVNADDLGYTPGVTRGILQAHREGIVTSATLMANAPDSEGA
                     GALVRRTADLDVGVHVVFTYGRALTRPDRIRSLVDEHGAFPRVTDLLRSGQPLAEHAL
                     VEARAQYERARELIGREPTHLDTHHWVHDMRALEDAMLALARETGTVLRAHDGRQRSR
                     FRDEGVRTTDRFVREFQHAGAIGVESLILLLELLDEQEGGTVELMCHPAEPDAALLEG
                     SSYAAERGVELRTLTDPAVAAAVERLGIELVTFAAL"
     gene            complement(2339..>2897)
                     /locus_tag="VFV20_01930"
     CDS             complement(2339..>2897)
                     /locus_tag="VFV20_01930"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEU5287134.1"
                     /translation="GDVVRGIIAGGDDALRTSLEGAEDDAGAGARDARRWVRPSDVAV
                     GISASGRARYVVGALATARDVGARTVALTCDATSPLASGADIAIALDVGPEVLAGSSR
                     LKAGPATKLVLNMLTTASMIRSGRTKGDLMIDLRATNAKLRERAVRIVRDETGADEAT
                     ARASLETSGWSVRAALDAMEKRAQR"
BASE COUNT          350 a         1046 c         1096 g          405 t
ORIGIN      
        1 gtgctgctct tctcgtcacg cggcaggctc tcgcgtgagg ccgccgcggc tgttggtcag
       61 ctccacgagc gtggagcgcg cgtgtacgcg gtcacgagcg acgcgcgtgt cgcggagcgc
      121 gcggacgatg cggtgctgct cgggtcgccg gtgggcgagg cgctcgcgcc tatcgcgctc
      181 gccgtcgtcg cgcagcacct cgcgcgggcc gtcgcgctgc ggcacgggcg cgacccggag
      241 cggcccgccg gcctttcgaa ggtgacgcgg acgctctagc ctgccgcgcg ggcttcaccg
      301 ccggagtgcg ctccggcaca gggcgcttct ccggcgccgg gcgctccttc tggccctcgg
      361 cctccgtctc gaacgagacc ttgaggtgcg tgccgtacgc cgtcgccacg cgctggagcg
      421 aacggagcga tggcaggtac cgcggatcct ccatgcgccg gagctgggtc tgcgacgtgc
      481 cgagcttctt cgccgcggcg cgctcggaga ggccggcgtc gcggcgcagc ttcccgaccg
      541 cggccgcgac ggaggcggca agagcgtcgg gccgcggctc ctcctgcggc ggggcgggtg
      601 cgaccggtgt tggtgtcggc tgatcgggca cgggcggtga ttctaggtcg gtcgctacgc
      661 ggcggcgaac gcgtcgcaca ggtcgggctc cgcggggtcg tactggaaga ggatcgcgcc
      721 ctcgcaaccc gcgtctttcg cggcccggac ctgggttcgg aggagcgccg gctcgcccgc
      781 gtagcccatg agcccgcccc agagctccgt ccgcggcgcc gccgcgcgcg ccaggccgag
      841 caggcgagcg acctccgtgc tgtcgcgccg gtacgccatc gggcagagga gatccacgac
      901 gccgtcggcg gcccagtccg gccatcgctg gagcacgcgg tcgcgggcga tcgccggatc
      961 ggggaacacc gccgccgaga tgaccaggtc cggccgcgcg ccgcggaggc gtgccgcgct
     1021 ctcgcgcacg aggtcggtga cgtgtcgatg gtcctccggc ccgccggcgt acgtgacctg
     1081 gggatagcgg acgtagtcgt agtggatccc ggcgacgtcg tagcgcgtcg cgatgtcggc
     1141 gaagacggtg atggtgtggc gtcgcgcggc tgtgttcgcc gggtcggtgt agatgccttc
     1201 ccagtcggtc ccgccggccg ggtcgagcgc gctcacgccg tcgggccgca gcagccagtc
     1261 cggatgcgcg ttcacgacgt gcgcctcgtc gcgcggcaga cggcgctcgg gatgcgacca
     1321 gacgaagtac acgttcgccc aggcgtggac gcgcgtgccg gtggaccggc cgcgctcgat
     1381 gaggtgagcg agggggtcga acccctcggc cagaccctct gcgcgtggct cgaggcgcgg
     1441 gctgtagtac gcgtccccac ggccacgcac ctgcacgacc agatcgacga tcccgcgcgc
     1501 cgccgccgcg gcgagaagag cgtcgatcgc atcgggtgtc gtgatcttgt cgcggacgac
     1561 ccacagcgcc ttcttcacag cgcggcgaac gtcacgagct cgataccgag acgctccacc
     1621 gccgcggcca cggcggggtc ggtgagcgtg cgcagctcga cgccgcgctc ggcggcgtac
     1681 gacgagccct cgagcaacgc ggcgtccggc tcggccgggt ggcacatgag ctcgacggtg
     1741 ccgccctcct gctcgtccag cagctcgagc aggaggatga gcgactcgac gccgatcgcc
     1801 ccggcgtgct ggaactcgcg cacgaagcgg tcggtggtgc gcacaccctc atcgcgaaat
     1861 cgcgagcgct ggcggccatc gtgcgctcgc agcaccgtcc cggtctccct ggcgagtgcg
     1921 agcatcgcgt cctcgagcgc gcgcatgtcg tgcacccagt ggtgcgtgtc gaggtgcgtg
     1981 ggctcgcggc cgatgagctc gcgcgcgcgc tcgtactgcg cgcgtgcctc gaccagggcg
     2041 tgctcggcga ggggctgccc gctccgcagc aggtccgtga cacgagggaa cgcgccgtgc
     2101 tcatccacga gcgagcggat ccggtcgggc ctcgtgagcg cccgcccgta ggtgaagacg
     2161 acgtggacgc cgacgtcgag gtccgccgtg cgccggacga gcgcgccggc gccctcgctg
     2221 tcgggcgcgt tcgccatgag cgtcgccgac gtgacgatgc cctcgcggtg ggcctggagg
     2281 atcccgcgcg tcacaccggg cgtgtagccg aggtcgtcgg cgttgacgat gaggcgcatc
     2341 agcgctgcgc tcgcttctcc atcgcgtcga gggcggcgcg cacgctccag ccgctcgtct
     2401 cgagcgacgc ccgagcggtc gcctcgtccg cgccggtctc gtcgcggacg atgcggacgg
     2461 cgcgctcgcg aagcttcgcg ttcgtcgcgc ggaggtcgat catgagatcg cccttcgtgc
     2521 gacccgatcg gatcatcgac gccgtcgtga gcatgttgag gacgagcttc gtggccgggc
     2581 ccgccttgag acggctcgat cccgcgagca cctcggggcc cacatccagc gcgatggcga
     2641 tgtccgcgcc ggatgcgagc ggcgacgtcg cgtcgcaggt gagcgcgacg gtgcgcgctc
     2701 cgacgtcccg ggccgtggcg agcgcgccga cgacgtagcg cgcccggccg ctcgccgaga
     2761 tcccgacggc cacgtcgctc ggccgcaccc accgacgcgc gtcgcgcgcg ccagcgccgg
     2821 cgtcgtcctc ggcgccctcg agcgaggtgc gcagcgcgtc gtcgccgccc gcgatgatcc
     2881 cgcgcacgac gtcgccg
//
LOCUS       DASTZR010000085         3273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_266553, whole genome shotgun sequence.
ACCESSION   DASTZR010000085 DASTZR010000000
VERSION     DASTZR010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266553"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..875
                     /locus_tag="VFV20_01935"
     CDS             48..875
                     /locus_tag="VFV20_01935"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEU5287135.1"
                     /translation="MPEWPDLHVLRGRIEKAVGGKRVTAVRVHDPLVLRATRPFDTAV
                     RGRVLDGVAHRGRFLVFALDDGTRVVVNPMLSGLFDLLPPETKIRATTAFALTFENGI
                     DLRHRDDTRMGKVYLLGPGETEAGVPQLASQGEEAGTLGWTADEFARRAKKTRRELRN
                     LLMDDEFIAGIGNAYADEILWAAKLHPKRKIASLDEGEMDRLRGALRATLERAAEIVE
                     AELPPQLGTKVRAHMNVRGRAGEPCPRCGAKIVRTRHGLDEMYVCPRCQPPPKGQLR"
     gene            complement(872..2158)
                     /locus_tag="VFV20_01940"
     CDS             complement(872..2158)
                     /locus_tag="VFV20_01940"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEU5287136.1"
                     /translation="MRGLRWPLRGYIAAVMAAAVVTALIAAAGCAPPSESGLAIGATL
                     LLMASLAQVWPLHLSTKVKITVDDLPTFAAALLLPPLDAMLIAAASTLIGLRFRNTRM
                     RWYNRCFNASATVLGTAAASLAYGLLHGPTPPEMSGTIAIIGAGAAKYVVQAVLVDLV
                     VALQLKRRPLDRWWELHRRDLPYETSLYLLGALAAVTVGDEPLALALFAVPMAAMLVS
                     MRETARVREQTRGAILELADLIDLRDPYTHGHSQRVAALAERLARRMKLETAQIALIR
                     DAARVHDIGKIGTNDLVLLKQGPLNESERAEMQRHAEIGHRLLSHIPEFFEGAELVLS
                     HHERHDGKGYPRGLKGDELPIEVSVISVADSYDAMTTDRPYRRGLPWSAVRSELLRGR
                     GSQWRERAVDLFVEMIEEDRARARGGAEIVPFRASA"
     gene            complement(2158..2628)
                     /locus_tag="VFV20_01945"
     CDS             complement(2158..2628)
                     /locus_tag="VFV20_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287137.1"
                     /translation="MILATLALIAAMVAGGTSAWAGPQWCEEDPEFIVNGSVVDVTTL
                     FPSDYARYVTGTVNFDMQVPTNVVAYALALPGTIPVTAQVRKTLPAYYGIGKIPVIVT
                     VTMSSSVGFQTYTQVTGTQGLLVSGIYGTATKPTYAKFSMYGTSLWNWSGGGDD"
     gene            2787..>3273
                     /locus_tag="VFV20_01950"
     CDS             2787..>3273
                     /locus_tag="VFV20_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU5287138.1"
                     /translation="MPNTIVVTREGAIAVVQLNRPAALNALNDELLGELAAALRELDD
                     DDSCRCIVITGSDKAFAAGADIKEAFVGSTPVTMLAHDLTAKWEVLRRIKTPLIAAVS
                     GYALGGGCELAMICDVIVASETAKFGQPEINLGIIPGAGGTQRLTRAVGKWVANELIL
                     TG"
BASE COUNT          526 a         1113 c         1167 g          467 t
ORIGIN      
        1 cgacgaagtc gggccggagc gagcgcgaca agtgaggaag gcacgaagtg cctgagtggc
       61 cggatctcca tgtgcttcgt ggccgcatcg agaaggccgt cggcgggaag cgcgtgaccg
      121 cggtccgcgt gcacgacccg ctggtgctac gcgcgacacg cccgttcgac acggccgtgc
      181 gcggccgggt gctcgacggc gtggcgcacc gtgggcgctt cctcgtgttc gcgctcgacg
      241 acggcacgcg cgtcgtggtg aacccgatgc tgtcaggtct cttcgatctc ctgccgcccg
      301 agacgaagat ccgcgccacg accgcgttcg cgctcacctt cgagaacggc atcgacctcc
      361 gccatcgcga cgacacgcga atgggcaagg tctatctgct cggcccgggc gagaccgagg
      421 caggcgtccc gcagctcgcg tcgcagggcg aggaggccgg cacgctcgga tggaccgccg
      481 acgagttcgc caggcgggcg aagaagaccc ggcgcgagct ccggaacctg ctgatggatg
      541 acgagttcat cgccggcatc ggcaacgcgt acgcggacga gatcctctgg gcggcgaagc
      601 tgcatccgaa gcgcaagatc gcgtcgctgg acgagggtga gatggaccgc ctgcgcggcg
      661 cgctgcgcgc gaccctcgag cgtgccgccg agatcgtcga ggccgagctg ccgccgcagc
      721 tgggcacgaa ggtccgggcg cacatgaacg tccgcggtcg tgcgggtgag ccatgcccgc
      781 gctgcggcgc gaagatcgtg cgcacgcgcc acggcctcga cgagatgtac gtgtgcccgc
      841 gctgccagcc gccgccgaag gggcagctcc gttaggcgct cgcccggaac ggaacgatct
      901 cggcgcctcc ccgcgctcgc gcgcggtcct cttcgatcat ctcgacgaac aggtccaccg
      961 cacgctcccg ccactgtgag ccgcggccgc gcagcagctc cgagcggacg gcgctccagg
     1021 gaaggccgcg gcggtacggc cggtctgtcg tcatcgcgtc gtacgagtcg gccaccgaga
     1081 tcacggacac ttcgatcggc agctcgtcgc ccttgagccc gcgcggatag cccttgccgt
     1141 cgtggcgctc gtgatgcgac aggacgagct cggcgccctc gaagaactcc gggatgtgcg
     1201 acagcagccg gtggccgatc tcggcatgac gctgcatctc ggcgcgctcg gactcgttga
     1261 ggggcccctg cttcagcagg acgaggtcgt tcgtcccgat cttgccgatg tcgtggacgc
     1321 gcgcggcgtc gcggatgagc gcgatctgcg ccgtctcgag cttcatccgc cgcgcgagcc
     1381 gctccgcgag cgcggcgacg cgctgcgaat ggccgtgcgt gtacgggtcg cgcaggtcga
     1441 tgagatcggc gagctcgagg atcgcgccgc gcgtctgctc gcggacgcgc gccgtctcgc
     1501 gcatcgagac gagcatggcg gccatcggga ccgcgaagag agcgagggcg aggggctcgt
     1561 cgcccacggt gaccgcggcg agcgcgccga gcaggtagag cgacgtttcg tacggcagat
     1621 cccgccggtg cagctcccac cagcggtcca gcgggcggcg cttcagctgg agcgcgacga
     1681 cgagatcgac gagcaccgcc tgcaccacgt acttcgcggc gcccgcgccg atgatggcga
     1741 tggtcccgct catctccggc ggcgtgggtc cgtgcagcag cccgtacgcg agcgacgctg
     1801 ccgccgtccc gaggaccgtg gcggacgcgt tgaagcagcg gttgtaccag cgcatccgcg
     1861 tgttgcggaa gcgcaggccg atgagcgtgc tcgccgccgc gatgagcatg gcgtcgagcg
     1921 gcggaagcag aagcgcggcg gcgaaggtcg ggaggtcgtc gacggtgatc ttcaccttcg
     1981 tcgacaggtg cagcggccag acctgtgcga ggctcgccat gagcaggagc gtggcgccga
     2041 tcgcgaggcc gctctccgaa ggtggcgcac agccggcagc cgcgatcagg gccgtgacca
     2101 cggccgcggc catcaccgcg gcgatgtagc cccgcagcgg ccagcggagg ccacgcatca
     2161 gtcgtcgccg ccgccggacc agttccagag cgaagtgccg tacatggaga acttcgcgta
     2221 ggtcggcttc gtcgcggtcc cgtagatgcc gctcacgagg agcccctgcg tgcccgtgac
     2281 ctgcgtgtag gtctgaaagc cgaccgacga gctcatcgtc accgtcacga tcaccgggat
     2341 cttgccgatg ccgtaatagg cgggcagcgt cttccgtacc tgtgcggtga ccgggatcgt
     2401 gccgggcagc gccagcgcgt aggccaccac gttggtaggc acctgcatgt cgaagttgac
     2461 cgtgccggtc acgtatctgg cgtagtcgga cgggaacagc gtggtcacgt ccacgaccga
     2521 gccgttcacg atgaattcgg ggtcctcttc gcaccactgc ggcccggccc atgccgacgt
     2581 gccgccggcc accatcgccg cgatgagcgc cagggtcgcc aggatcaagc gcgtgctcga
     2641 catttccgcc tcccacccat gtcagcgcac gttcagcgcc ggaccggagg actattggct
     2701 tggcggcacg cggacgcgtg tcggcgaagt cgcagaaaat ctcaaaacac ggtaaacgcc
     2761 ggccgcacga acggcctgga gggaacatgc cgaacacgat cgtggtcacg cgcgagggcg
     2821 cgatcgccgt cgtccagctg aaccgtccag cggcgctgaa cgcgctgaac gacgaattac
     2881 tcggcgagct cgccgccgcg cttcgtgagc tcgatgacga tgactcatgc cgctgcatcg
     2941 tcatcaccgg aagtgacaag gcgttcgcgg cgggcgcgga catcaaggag gcgttcgtcg
     3001 ggtcgacgcc ggtgacgatg ctggcgcacg acctcaccgc caagtgggag gtgctgcggc
     3061 gaatcaagac ccccctcatc gccgccgtct ccggctacgc gctcggcggt ggctgcgagc
     3121 tcgcgatgat ctgcgacgtc atcgtcgcgt ccgagaccgc gaagttcggg cagccggaga
     3181 tcaacctggg catcatcccc ggcgccggcg ggacgcagcg gctcacacgc gcggtgggga
     3241 agtgggtggc gaacgagctc atcctcaccg gcc
//
LOCUS       DASTZR010000086         4124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_684249, whole genome shotgun sequence.
ACCESSION   DASTZR010000086 DASTZR010000000
VERSION     DASTZR010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684249"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VFV20_01955"
     CDS             complement(<1..192)
                     /locus_tag="VFV20_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287139.1"
                     /translation="MIELWAGHGGTGDPRATRRSLRIGDLGERASGRVLVIDDDPSIL
                     DTVSAILSGEGYQVMSAPGG"
     gene            complement(195..2033)
                     /locus_tag="VFV20_01960"
     CDS             complement(195..2033)
                     /locus_tag="VFV20_01960"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5287140.1"
                     /translation="MTALELITASTHAIYVLVFVLVFLRTVRQPTPAHLDMTAFFGVV
                     ALIVLESRIVPLVGGTPPVWMRDAVIILLMAMPYLLLRLVNDFTIVPTVITRLVEVGF
                     IAFIVLTVSLDAALPPTLALVYVAYFAIVSLYCAARFVGAGRRALGVTRRRLEAVSLG
                     SILLGGDLVFAGLGAFLAEPERTVVSAMTQLMGLGSALSYYVGFAPPVVLRRAWQAPE
                     LRAFLTRAAQLPRLPTTLDIVRELEAGAAASTGSSARIGLWNEDDRVIRMWQPGNDEA
                     VEVKPGQFLAGRAFERQSVLYSGDPVSDDPENAARYRNAQVGAVIAAPITAGGRRLGV
                     LVLFATRPPIFALSDLELAQLLADQASVILESRALIDHAARVKAREEATRMKEDFVSA
                     AAHDLKTPLTTVVAQAEFLERKALRDPAAPPDVPGLQRIVRESKRLAALVTDLLDASR
                     LEQGKLVGEREPVDVGALVTEVVGRQQPGIHLCDVDVRGAVVGTYDRRRIEQLVENLV
                     ENAKKYSPEAARVRVEVWQQDGEARIVVRDQGIGIPAADLPTIFERFARASNVDDRKF
                     HGMGLGLYICRGIVEEHGGRIWAESEMGKGSTFHVALPLREGRRLN"
     gene            complement(2112..2627)
                     /locus_tag="VFV20_01965"
     CDS             complement(2112..2627)
                     /locus_tag="VFV20_01965"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEU5287141.1"
                     /translation="MTELGLFLLLLKDSALALGGLGSLPLIRQDLVAAGVATDAQVVE
                     AIAIGRLSTGPSGLWIVSLGYLVAGWAGATLALVASCLPPLVIVPLTIAARRYLLTAP
                     FAGLVRGATLATAGLLASTGLSLVAPDLGGTAGWQYAVAALAVAVTYDGRVHPALVVI
                     GGAAAGIVLGR"
     gene            complement(2624..3184)
                     /locus_tag="VFV20_01970"
     CDS             complement(2624..3184)
                     /locus_tag="VFV20_01970"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEU5287142.1"
                     /translation="MRDLLTAWIAVGTQSVGGGASTLLLIRREFIDRRRWITGREFAE
                     AWALSQLSPGIHLVALAGLLGQRIAGWRGVVLSVGGMMVPAAIVTALMTAAYATIADQ
                     PLARAALAGMAPATGGMTIGLALQMTRDARRHGLFVIVDAGVVLVAFAVLTFTGASSI
                     VVIVAAAALGSLLLRKERPTSDRARE"
     gene            3207..3938
                     /locus_tag="VFV20_01975"
     CDS             3207..3938
                     /locus_tag="VFV20_01975"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEU5287143.1"
                     /translation="MGASVRIGTSGWVYPHWRGAFYPKGLPTTRWMEFYTARFDTVEL
                     NASFYRQPTREQFVRWRRAVPEGFQFAVKLNRYISHVKRLTVDAATVARSYDPVAGLG
                     PALGAILVQLPPRFQFDPDRLDGFCRAVARRRRRHAIEPRDPSWLTDPALDALRSRHV
                     ALCIIDARRWPTRIAITGDFVYLRFHGPEALYASRYDDAALLEWAGRIRAWRDDGFDV
                     FAYFNNDTHAYAPRDAERLRELVGS"
BASE COUNT          669 a         1421 c         1449 g          585 t
ORIGIN      
        1 gcctcccggc gcggacatca cctgataacc ctcgcctgag aggatcgcgc tcacggtatc
       61 gaggatcgac ggatcatcgt cgatcacgag cacgcgcccg ctcgcgcgct cgccgaggtc
      121 accgatgcgc agcgagcgcc gcgtcgctcg agggtcgccg gtgccaccgt gccccgccca
      181 caactcgatc atcctcagtt cagtctcctt ccttcacgta gaggtaacgc gacatggaac
      241 gtcgatccct tgcccatctc gctctcggcc cagatgcgcc cgccgtgctc ttcgacgatg
      301 cctctgcaga tatagagccc cagccccatg ccgtggaact tccggtcgtc gacgttcgac
      361 gcccgcgcga accgctcgaa gatcgtggga aggtcggccg cggggatccc gatgccttgg
      421 tcgcgaacga cgatgcgcgc ttcaccgtcc tgctgccaga cctcgacccg gacgcgggcc
      481 gcctcggggc tgtacttctt cgcgttctcg acgaggttct ccacgagctg ctcgatgcga
      541 cggcggtcgt acgtgcccac gaccgcgccg cgcacgtcga cgtcgcacag gtggatgccc
      601 ggctgctgcc gcccgacgac ctcggtcacg agcgcgccga catcgacggg ctcacgctct
      661 ccgacgagct tgccctgctc gaggcgtgac gcgtcgagca ggtcggtgac gagggcggcc
      721 agccgcttcg attcgcggac gatgcgctgc aggcccggca cgtcgggtgg cgccgctgga
      781 tcgcggagcg ccttccgctc gaggaactcg gcctgcgcga cgaccgtcgt gagtggcgtc
      841 ttgagatcgt gcgcggccgc cgacacgaag tcctccttca tgcgggtggc ctcttcccgc
      901 gccttcaccc gtgcggcgtg gtcgatgagc gcgcggctct cgaggatcac agaggcctgg
      961 tccgcgagca gctgcgcgag ctcgaggtcg ctgagcgcga agatcggcgg ccgtgtcgcg
     1021 aagagcacca ggacgcccag acggcgtccg cccgcggtga tcggcgcggc gatcaccgca
     1081 ccgacctgcg cgttgcgata gcgcgcggcg ttctccggat cgtccgacac cgggtcgccc
     1141 gagtagagga cgctttgccg ctcgaaggcc cggcccgcga ggaactgccc cggcttcacc
     1201 tcgaccgctt cgtcgttccc cggctgccac atgcggatga cgcggtcgtc ctcgttccac
     1261 aggccgatgc gtgcgctcga tcccgtgctc gccgccgcgc ccgcctcgag ctctcggacg
     1321 atgtcgagcg tcgtcggcag ccgcgggagc tgggccgcgc gcgtgaggaa cgcgcgcagc
     1381 tcgggcgcct gccacgcgcg ccgcagcacg accggcgggg cgaatccgac gtagtacgac
     1441 agcgccgagc cgaggcccat gagctgcgtc atcgcgctca cgacggtgcg ctcgggctcc
     1501 gcgaggaacg cgccgagacc agcgaagacc agatcgccgc ccagcaggat cgaaccgagc
     1561 gagacggcct cgaggcgccg gcgcgtcacg cccagcgcgc ggcgtcccgc gccgacgaat
     1621 cgtgccgcgc agtagagcga cacgatcgcg aagtacgcca cgtagacgag cgcgagcgtg
     1681 ggcggaaggg cggcgtcgag agacaccgtg aggacgatga acgcgatgaa gcccacctcg
     1741 acgagacggg tgatgacggt cggcacgatc gtgaagtcgt tgacgagccg cagcagcagg
     1801 tacggcatgg ccatgagcag gatgatcacc gcgtcgcgca tccagaccgg cggtgtcccg
     1861 ccgacgagcg ggacgatgcg cgactcgagc acgatgaggg cgaccacgcc gaagaacgcc
     1921 gtcatgtcca ggtgagcggg cgtgggctgc cgcacggtgc ggaggaatac gagcacgaac
     1981 acgagcacgt agatggcgtg cgtgctcgcg gtgatcagct cgagtgcggt catcttcctc
     2041 ggtgcgtgat tctaggaagc gggctccgtt cgagcacgca ccacacgcgt gacgcgcgcc
     2101 gagcggccga cctaccgacc gaggacgatg cccgccgccg ccccaccgat cacgacgagc
     2161 gcggggtgga cgcgtccgtc gtaggtgacc gcgaccgcca gtgccgcgac cgcgtactgc
     2221 caccccgccg ttccgccgag gtccggcgca acgagcgaaa gccccgtcga tgccagaagg
     2281 ccggcggtcg cgagcgtcgc cccgcgcacg aggccggcaa acggcgcggt gagcaggtag
     2341 cggcgcgcgg cgatcgtgag cggcacgatg acgagcggcg gcaggcagct ggcgacgagc
     2401 gcgagcgtcg ccccggccca gccggcgacg aggtagccga ggctcacgat ccagagaccg
     2461 ctcgggccgg tggagaggcg gccgatggcg atcgcctcga cgacctgcgc gtcggtcgcc
     2521 acgccagcgg cgacgaggtc ctgacggatc agcggcagcg atccgaggcc gccgagcgcc
     2581 agcgcagaat ccttcaggag caagaggaag agcccgagct ccgtcattct cgcgctcggt
     2641 ccgacgtggg gcgttccttg cgcagcaaca gcgagccgag cgcggcggcc gcgacgatca
     2701 cgacgatgct cgatgcgccg gtgaaggtga ggaccgcgaa cgcgacgagc acgacgcccg
     2761 cgtcgacgat cacgaacagg ccgtggcggc gcgcgtcgcg tgtcatctga agggcgagcc
     2821 cgatcgtcat accgccggtg gccggtgcca tgccggcgag cgccgcacgc gcgagcggct
     2881 ggtcggcgat cgtcgcatac gccgcggtca tgagcgcggt gacgatggcg gccggcacca
     2941 tcatgccgcc gaccgagagc acgacgccgc gccacccggc gatccgctgg ccgaggaggc
     3001 cggccagtgc gacgaggtga atgcccggtg agagttgcga cagcgcccag gcctccgcga
     3061 actcgcggcc ggtgatccag cgacggcgat caatgaactc gcgacggatg agcaggagcg
     3121 tgctcgcgcc gccgccgacc gactgcgtgc cgacggcgat ccacgccgtg aggagatcgc
     3181 gcatgtgtgc gacgtaggct aggtcgatgg gcgcatcggt gcgcatcggc acgtcgggat
     3241 gggtgtatcc gcattggcgc ggagccttct atccgaaagg ccttccgacc acgcggtgga
     3301 tggagttcta cacggcgaga ttcgacaccg ttgagctgaa cgcctcgttc tatcgccagc
     3361 cgacgcgcga gcagttcgtc cggtggcggc gagcagttcc cgagggcttc cagttcgccg
     3421 tgaagttgaa ccgctacatc tcccatgtga agcggctcac cgtcgacgcg gcgaccgtcg
     3481 cgcgttcgta cgacccggtc gccggccttg gtccggccct cggggcgatc cttgtgcagt
     3541 tgccgccgcg tttccagttc gatccggatc gccttgacgg attctgtcga gcagtcgcgc
     3601 ggcgtcgtcg ccgccacgcc atcgaaccgc gcgatccgtc gtggctgacc gacccggcac
     3661 tcgacgcact acggtcccga cacgtcgcgt tgtgcatcat cgacgcgcgg cggtggccca
     3721 cgcggatcgc gatcacgggg gatttcgtct acctccgctt ccacggtcca gaggcgttgt
     3781 atgcgtcgcg gtacgacgac gcggctctgc ttgaatgggc ggggcgcatc cgcgcatggc
     3841 gggatgacgg gttcgacgtg ttcgcgtact tcaacaacga cacgcatgcg tacgcgcccc
     3901 gcgatgccga acgattgcgg gagttggtcg gcagctaaga gtgacggtcg gaccccgaag
     3961 tgacgtagtc gcgccgcgat catccacacg tgagggcccg tagagccgcc gtgagaccga
     4021 aaggtcccct agagtactcg gcacatggcg ccggtcggct acctcgaagc tggggtacgc
     4081 gtgacaccgt gagtcgggat cgggcggcga tgaaccgtcc tgtc
//
LOCUS       DASTZR010000087         4933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_919326, whole genome shotgun sequence.
ACCESSION   DASTZR010000087 DASTZR010000000
VERSION     DASTZR010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919326"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="VFV20_01980"
     CDS             <1..979
                     /locus_tag="VFV20_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5287144.1"
                     /translation="TLAAIRGRAIDLVLAETPPLPLAVTAAAIARRNGVPLVVYVADL
                     WPDVALAMGALREGWLADRLRSLEAFVYRNAARITVPTEGLFAALTAHPAAGPGKVLL
                     LPNGVDPDVFHPIPAADCEVERTALGLAGRALFLYAGTIGHAQALDTIVDTAVALRDR
                     SDIVFAFLGDGPERGRLEGRTRALRLDNVVFAGAVPPEHVARYLSLARATIAPLRAVD
                     LFAATRPAKILPSLACARPVIFCGRGEMAAIIEREGCGVVVPPEDPARLADAVRRLAD
                     SDEDARAMGERGQTWALRDYDFRGLVRRWWDAVSEGLPRPVRDSGTRAS"
     gene            complement(927..2231)
                     /locus_tag="VFV20_01985"
     CDS             complement(927..2231)
                     /locus_tag="VFV20_01985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEU5287145.1"
                     /translation="MKDHDELQRRISAKQARVGVVGLGYVGIPVAVSFAAAGFDVVGV
                     DRSPERVDALNGGTLPLATNEPGLEPLLEAARSAGRFRATSDELALADREIAIVAVDT
                     PVAEHRPDTRQLADACRIVSQRVRPPALVIIESTIAPRTMREIVAPLFGDGIFLVHCP
                     ERVRPGRLVRNLRDMARLVGVTEPRVGVLATELYRNIVRADLLVSDWETAEVIKTAEN
                     ATRDVQIALANQLAIISDHAGVDVRKVREQINRLWAAEPLVLEPGPGVGGHCLPKDPW
                     LLVSGLPPEASRALIEGARALNESMPSHTARVIARALADEGVDVSGAVVAVLGLTYEA
                     NSDDQRNAPGPRVAAEIERMGGRIVRHDPFVTRDDVSAAVRDADATVVVVPHSIYREA
                     DWDVLGGLMRHRVFVDCRRAFEADALKMLGYRYRALGVAALR"
     gene            2309..2998
                     /locus_tag="VFV20_01990"
     CDS             2309..2998
                     /locus_tag="VFV20_01990"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEU5287146.1"
                     /translation="MPPRRVKLDMHTHSEYSPDSRTPLAAQAAALVAAGIDVVCATDH
                     NTIEGALRLREIEPDRLRVIVGEEISSRDGEIIGLFLEKQVPRDLSAEETIARIHEQG
                     GLVSVPHPFSRNRLYHLRRDALERVYAQIDCIEIFNAREAFAADNRRAEAFAKEHGLP
                     GAVGSDAHRASEIGHAWLEVDEFAGRDDFVAAVRGGEVHGRLTGSGIHLATRYDVFRK
                     WLSRRRTTIRS"
     gene            2962..4029
                     /locus_tag="VFV20_01995"
     CDS             2962..4029
                     /locus_tag="VFV20_01995"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEU5287147.1"
                     /translation="MAQQTKNDDPLLDASSRHVEDPTIEQLAEEAGHTDEVAVRRTLF
                     GWRELVSTLIPLALIAVAARNVDWSEAGRALGEARPYYVLLALVVYYATFPLRSWRWA
                     RLLREGGADLGWGDLLRILFLGWFVNSIVPAKLGDLYRSFLVKRRFGISLSRTVGVVV
                     AERLLDLLVVFVLLIVGGYVAFGRTILPDLTIVYVTGAALAVAIVAGIAAMYYLAPKL
                     ARFFPKEARRIGRLFREGVLHSFRALPLAGPLTVVIWSAEALRLFFVLTSLGLELPAS
                     GVVFVAVAVALLTTVPLTPAGFGFVEIAMVYVLTTGFGLAQADAIAVAVVDRAVSVLS
                     VIIVGAIVYLRTSHEERLGAR"
     gene            4026..>4933
                     /locus_tag="VFV20_02000"
     CDS             4026..>4933
                     /locus_tag="VFV20_02000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEU5287148.1"
                     /translation="MTLTAPTRPIFLLAVATLLILAGVLAARGAFWYQSADFFCLYQG
                     ARSLAEGRDPYDISWWHSVTGGVYPDPWRGHAASSCGNRYAYPLWTAAAMAPIGILPL
                     ELAASAWMALSIGAAVFGAAALWRSHGAPRRNAALFAALVFTSQPFWVLLVGGQMTGI
                     MLGLVGALALWVARARESAAGLALALLALKPQLVVLTIPSIALRALIDRRRRIVTTAV
                     AAGIAMVGVPTLLVPAWPLEWLRDVGTSRVLDIGPLATAWGAATDLLGHPAWGIVFVA
                     LVVGPCLLLARGLDMSSVLALSLPLSL"
BASE COUNT          650 a         1767 c         1694 g          822 t
ORIGIN      
        1 gacgctcgcg gcgatccgcg gtcgcgcgat cgacctcgtc ctcgcggaga cgccgccgct
       61 tcctctggct gtgacggcgg cggcgatcgc gcgacgcaac ggcgtgccgc tcgtcgtgta
      121 cgtcgcggat ctgtggcccg atgtcgcgct cgcgatgggc gcgctgcgcg aaggctggct
      181 tgccgatcgc ctgcgctccc tcgaggcgtt cgtctaccgc aacgcggccc ggatcaccgt
      241 cccaacggag ggcctcttcg cggcgctcac cgcgcatccg gccgccggcc ccggcaaggt
      301 gcttctcctc ccaaacgggg tcgatcccga cgtcttccat cccatcccgg ccgcggactg
      361 cgaggtcgag cgcacggcac tcgggctcgc aggtcgcgcc ctctttctct acgcgggcac
      421 gatcggtcat gcgcaggcgc tcgacacgat cgtggacacc gctgtggcgc tacgcgaccg
      481 atcggacatc gtgttcgcat tcctcggtga cgggcctgag cgtggacgcc tcgagggccg
      541 cacgcgcgcg ctccgactcg acaatgttgt gttcgcgggc gcggtgcccc cggagcacgt
      601 ggcgcgctac ctctcgctcg cgcgagcgac gatcgcgccg ctccgggcgg tcgacctgtt
      661 cgccgctacc cgtcccgcga agatcctgcc atccctcgcg tgcgcgcgcc ccgtcatctt
      721 ctgcggtcgc ggtgagatgg cggcgatcat cgagcgtgag ggctgcggtg tggtcgttcc
      781 accggaggac cccgcacggc ttgccgacgc agtgcgtcgc ctcgccgact ccgatgagga
      841 cgcgcgtgcc atgggggagc ggggacagac gtgggcgctc cgcgactacg acttcagggg
      901 tctcgtccgt cgatggtggg acgcggtcag cgaagggctg ccacgcccag tgcgcgatag
      961 cggtacccga gcatcttgag cgcgtcggcc tcgaacgcgc gacggcagtc gacgaagacc
     1021 cgatggcgca tgagcccgcc gagcacgtcc cagtccgcct cgcgatagat gctgtgcggg
     1081 accacgacga cggtcgcgtc cgcgtcgcga acggcggccg agacatcgtc gcgcgtcacg
     1141 aacggatcgt gacgcacgat cctgccgccc atccgttcga tctcggccgc gacgcgagga
     1201 ccgggagcgt tgcgctggtc gtccgaattc gcctcatacg tgagaccgag gacggcgacg
     1261 acggcgccac tcacgtcgac tccttcatcc gcgagagcgc gtgcgatgac ccgtgccgtg
     1321 tgcgacggca tgctctcgtt cagagcgcga gcgccctcga tgagagcgcg ggaggcctcg
     1381 ggcggcagac ctgagacgag gagccatggg tccttcggca ggcagtgccc gcccacgcct
     1441 gggcccggct cgagcacgag cggctctgcc gcccacaggc gattgatctg ttcacgcacc
     1501 ttccgcacgt cgaccccggc gtgatcgctg atgatcgcga gctgattcgc gagcgcgatc
     1561 tggacgtcac gcgtcgcgtt ctccgccgtc ttgatgacct ccgcggtctc ccagtcgctc
     1621 acgaggaggt cggcgcgcac gatgttgcgg tacagctcgg tcgcgagcac cccgacgcgc
     1681 ggctcggtga cacccaccag ccgggccatg tcccgcagat tacggacgag tctgcccgga
     1741 cgaacgcgct cgggacagtg aacgaggaag atgccatcgc cgaacagcgg agcgacgatc
     1801 tcgcgcatcg tccgcggcgc gatggtcgat tcgatgatga cgagcgcggg cggtcgcacg
     1861 cgctgcgaaa cgatgcggca cgcatcggcg agctgacggg tgtcgggccg gtgttcggcc
     1921 accggagtgt ccacggcgac gatcgcgatc tcacggtccg cgagcgcgag ctcgtccgac
     1981 gtcgcacgga agcgtccagc gctacgcgcc gcctcgagca gcggctcgag cccgggctcg
     2041 ttcgtcgcga gcggcagtgt gccgccgttc aacgcatcga cgcgctccgg cgagcggtcg
     2101 acaccgacga cgtcgaaccc agccgcggcg aacgacactg cgacggggat gccgacgtag
     2161 ccaagaccga cgacgccgac ccgtgcctgc ttcgccgaga tgcgacgctg tagctcgtcg
     2221 tggtccttca tccgcgacag gctatcgcgc gtgcgtatcg gcctcgcgtc agccagcggg
     2281 tggaggtcgc ctgacgtatc gtgccgacgt gcccccacgc cgtgtgaagc tggacatgca
     2341 cacacatagc gagtactccc cggacagccg cacgccgctc gcggcgcagg ccgcggccct
     2401 cgtcgcggct gggatcgatg tcgtgtgcgc gaccgatcac aacacgatcg aaggggcgct
     2461 gcggctccgc gagatcgagc cggaccgcct gcgcgtcatc gtcggcgagg agatctcgag
     2521 ccgcgatggc gagatcatcg ggctgttcct cgagaagcag gtcccgcgcg acctgtccgc
     2581 cgaggagacc atcgcgcgga tccacgagca gggcggtctc gtgtcggtgc cccatccctt
     2641 cagccgcaac cgcctctatc acctgcggcg cgacgctctc gagcgcgtgt acgcccagat
     2701 cgactgcatc gagatcttca atgcgcgcga ggcgttcgcc gcggacaacc gccgcgccga
     2761 ggccttcgcg aaggagcacg gactgcccgg cgccgtcggc agcgacgcgc accgcgcatc
     2821 ggagatcggt cacgcctggc tcgaggtgga cgagttcgcg ggacgggacg acttcgtcgc
     2881 cgcggtgcgc ggcggcgagg tgcatggccg cctcaccgga tcggggatcc acctggcgac
     2941 gcggtacgac gtgttccgga aatggctcag cagacgaaga acgacgatcc gctcctagac
     3001 gcgtcctcga ggcacgtcga ggaccccacc atcgagcagc tcgccgaaga ggccgggcat
     3061 accgacgagg tcgccgtacg tcggacgctc ttcggctggc gagagctcgt gtcgaccctc
     3121 atcccgctcg cgctcatcgc ggtcgcggcg cggaacgtcg actggagcga ggccgggcga
     3181 gcgctcgggg aggcgcgacc ctactacgtg ctgctcgcgc tcgtcgtgta ctacgcgacg
     3241 ttcccactgc gctcatggcg gtgggcgcgc ctcctgcggg agggcggtgc ggacctcgga
     3301 tggggagacc tgctgcgcat cctcttcctc ggctggttcg tgaactcgat cgtgcccgcg
     3361 aagctcggcg acctctaccg gtcgttcctc gtcaagcgcc gcttcggcat ctcgctgtcg
     3421 cgcaccgtcg gcgtcgtcgt cgccgagcgg ctgctcgacc tgctcgtcgt gttcgtgctg
     3481 ctgatcgtcg gcggctacgt ggccttcggc cgcacgatcc tgcccgacct cacgatcgtg
     3541 tacgtcaccg gcgcggcgct cgccgtggcg atcgtcgcgg ggatcgccgc gatgtactac
     3601 ctcgcgccaa agctcgcgcg cttcttcccc aaagaggcgc ggcgcatcgg ccgcctcttc
     3661 cgggagggcg tgctccacag ctttcgcgct ctgccgctcg ccgggccgct cacggtcgtc
     3721 atctggtcgg cggaggcgct gcggttgttc ttcgtcctca cgtccctcgg gctcgagctc
     3781 cccgcttcgg gcgtcgtgtt cgtcgccgtc gcggtggcgc tgctcaccac ggtgccgctc
     3841 acacccgcgg ggttcggatt cgtcgagatc gcgatggtct acgtgctcac gacaggcttc
     3901 gggctcgccc aggccgacgc catcgccgtt gccgtcgtcg accgcgccgt gagcgtcctc
     3961 tcggtcatca tcgtcggcgc gatcgtgtac ctgcgcacga gccacgagga gcggctggga
     4021 gcgcgttgac gctgaccgcg ccgacccgac cgatcttcct gctggccgtc gcgacgctgc
     4081 tcatcctcgc gggcgtgctc gccgcgcgtg gcgcgttctg gtaccagagc gccgatttct
     4141 tctgcctcta tcagggcgcc cgctcgctcg cggagggccg cgacccctac gacatttcgt
     4201 ggtggcactc cgtcaccggc ggtgtctacc ccgatccgtg gcgagggcat gcggcgtcgt
     4261 cgtgcggcaa ccgctacgca tatccgctct ggaccgccgc ggcgatggcg ccgatcggga
     4321 tcctaccgct cgaactcgcg gcctcggcgt ggatggcgct ctcgatcggt gcggccgtat
     4381 tcggcgctgc ggcgctgtgg cgatctcacg gtgcgcctcg caggaatgcc gcgctctttg
     4441 cggcgctcgt cttcacgtcg cagccgttct gggtgctgct cgtcggggga cagatgaccg
     4501 ggatcatgct cggtctggtc ggcgcgctcg cgctgtgggt ggcacgtgcg cgtgagtccg
     4561 cggcggggct tgcgctcgcc ctcctggcgc tgaagccgca gctcgttgtg ctcacgatcc
     4621 catcgatcgc cctgcgcgca ctgatcgatc gacgccgccg gatcgtcacc accgccgtcg
     4681 ccgccggcat cgccatggtc ggcgtgccga ccctgctggt cccggcgtgg cccctcgagt
     4741 ggttgcgcga tgtcggaacg tcgcgcgtgc tggacatcgg ccccctcgcg acggcctggg
     4801 gggccgcgac cgatctcctt gggcatcccg cgtggggcat cgtgttcgtc gcgctcgtcg
     4861 tcggcccctg cctgttactc gcgcgcggcc tggacatgtc ctcggtactc gcgctatcgc
     4921 tcccgctctc gct
//
LOCUS       DASTZR010000088         3131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_136160, whole genome shotgun sequence.
ACCESSION   DASTZR010000088 DASTZR010000000
VERSION     DASTZR010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136160"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VFV20_02005"
     CDS             complement(<1..773)
                     /locus_tag="VFV20_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287149.1"
                     /translation="MARLAWLGGLAAMAALALLIGLRGQAAASDTASCTQVDGYLSFC
                     LNVPLSGVRRETSVPLIALSASRADVRVETTVPAADATALTTDVDRSVERVEALFGHA
                     FDPRPRVLLFGTGASFATAAGELFGYSTATAAYVASTYGGIFDRPTLTIALNWSASSP
                     ERMSAAIEHELGHLMIRQLARGRDVPAWLDEGIATLIEEDASAAAHWGADEALVGHAV
                     GSGRIASLRDLGRISDFHAAYAKVGRPLYAYVATAVRAM"
     regulatory      complement(785..859)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1036..1599
                     /locus_tag="VFV20_02010"
     CDS             1036..1599
                     /locus_tag="VFV20_02010"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5287150.1"
                     /translation="MKQALRLLALAILGVTIAACTGGTPSASTQAPPNETAIDALNRG
                     LELHRAGKLYEATVAYFQSLAKEPANQFAFYNLGLIAQQTQKTTIAESYYRIALDIDP
                     KFASPLYNLAILRHNAGAYTEVVSLYRRLLEIEPSNANAHYNLALALRALGQRTDADA
                     EFARARQLDSRLVPPTGSESPRPTATR"
     gene            1620..>3131
                     /locus_tag="VFV20_02015"
     CDS             1620..>3131
                     /locus_tag="VFV20_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287151.1"
                     /translation="MFVAAPVLFNAIALLPEVGTRVASINDDAFHWAFISRGVDAIEH
                     GENVLEFWVPQMGLGFPQFLYYQHLPHVSVILLYEALFRSVDAYTLFNVVRYALLAGL
                     PVTVFWALRRMDLSPMSAAVGAAASSVLSTPYLYGFDYNSYLWRGFGMYTQLWATHLS
                     FVTLACVYRLIQRGTGHVAAILTLSALALSHILYAYMAIITMGVVLLVGISRANALAR
                     AARLAIVGTATAVITSYMWIPTIQSAGLYLDFSPYLERWKYDSFGAARVLEYFAKGQL
                     FDEGRPGVLSALVVAGLLAAAALRTPAAITAIALFATWLVLFFGRPTLGPLAALFPLN
                     DALYFHRFIGGVHLAAIVLIGIAGGYALDRARGTPARVAVSVLLIALLLPAFAERASY
                     YATQTGWMRTTADAIDADADLRLVLTTLSALPPGRVFAGKNDDWGPQLNFGVPFRSTQ
                     VRHMLTFTGIPEASIPYSGPSLNADLLYDFDERRAELYDLFDARYVIAPTGRAM"
BASE COUNT          467 a         1117 c         1023 g          524 t
ORIGIN      
        1 tccatcgcgc ggacggcggt ggcgacgtac gcgtagagcg gacgtccgac cttcgcgtat
       61 gccgcatgga agtcggagat ccgtccgagg tcgcgcagcg aagcgatgcg acccgagccg
      121 acggcgtggc cgaccagcgc ctcgtcggcg ccccagtgcg cggcggccga cgcatcctcc
      181 tcgatgagcg tcgcgatccc ctcgtcgagc catgcgggta cgtcgcggcc acgcgcgagc
      241 tgccggatca tgagatggcc gagctcgtgc tcgatcgcgg cgctcatccg ctcggggctc
      301 gaagcgctcc agttgagggc gatggtcagg gtcgggcggt cgaagatgcc gccgtaggtg
      361 ctcgcgacgt acgcggcggt cgccgtcgag tagccgaaga gctcacccgc cgcggtcgcg
      421 aacgacgcgc ccgtgccgaa gaggaggacg cgcggacgcg ggtcgaaggc atgtccgaag
      481 agggcctcga cgcgctcgac ggagcggtcg acgtcggtcg tgagggctgt cgcgtcggcc
      541 gccgggaccg tcgtttcgac gcgcacgtcc gcgcgtgatg cggagagggc gatgagaggg
      601 acgctggtct cgcggcgcac gccgctcagg gggacgttga ggcagaagct gaggtagccg
      661 tcgacctgcg tgcagctggc ggtgtcactc gccgcagcct gaccgcgaag gccgatgagc
      721 agcgcgagcg ccgccatcgc cgcgagcccg ccgagccatg ccaagcgcgc catcccaccc
      781 tcgcttcggt aaccccgcta actcggtgag tccggaggct ttgcgtcccc gcctcgcgac
      841 gggtttgccg tttcgtgctg gacccggacc accacccggg tcgccactca cgttacgccc
      901 cggggggcgc acgaacaggg gcgaggaagt cctggacggt cggcggtcgt agtaggcccg
      961 acgcactacg ccagatgaag gactcccccg gtggtacgag gaggactagg cggtctgctg
     1021 gctatgatgc cggcaatgaa acaggcgctc cgcctccttg cgctcgcgat cctcggggtc
     1081 acgatcgcgg cctgtactgg cgggacgccc tccgcctcga cccaggctcc gccgaacgaa
     1141 acggcgatcg atgcgctgaa tcgcggcctc gagctccacc gcgccgggaa gctgtacgag
     1201 gcgaccgtcg cgtacttcca gtcgctggcc aaggagccgg ccaaccagtt cgccttctac
     1261 aacctcggcc tcatcgcaca gcagacgcag aagaccacga tcgcggagag ctattaccgg
     1321 atcgcactcg acatcgatcc gaaattcgcg tcgccgctct acaacctcgc gatccttcgg
     1381 cacaatgccg gcgcgtacac cgaggtggtg agtctctatc ggcggcttct cgagatcgag
     1441 ccgagcaacg cgaacgccca ctacaatctc gcgctcgcgc tgcgcgctct cggtcagcgc
     1501 acggatgcgg acgccgaatt cgcgagggcg cgacagctcg attcgcggct cgtcccgccg
     1561 accggcagcg aatcgccacg gccgaccgcc acgcgctagc cgcgcgcgtc gcgccgtggg
     1621 tgttcgtcgc ggcacccgtc ctcttcaacg cgatcgcgct ccttcctgag gtcgggaccc
     1681 gcgtcgcgag catcaacgac gacgccttcc actgggcgtt catctcgcgc ggcgtcgacg
     1741 cgatcgagca cggcgagaat gtgctcgagt tttgggtccc gcagatgggt ctgggattcc
     1801 cgcagttcct ctactaccag cacctgcccc atgtctccgt gatcctgctc tacgaggcgc
     1861 tcttccggag cgtggacgcg tacacgctgt tcaacgtcgt gcgctatgcg cttctggcgg
     1921 gcctgccggt cacggtcttc tgggctctcc gccgcatgga tctctctccg atgagtgcgg
     1981 ccgttggagc ggccgcgtcg tcggtcctga gcacgcccta tctctatggc ttcgactaca
     2041 acagctatct ctggcgcgga ttcgggatgt acacccagct ttgggcaacg cacctctcgt
     2101 tcgtcacgct cgcgtgcgtg taccgcttga tccagcgcgg gaccggacac gtcgccgcca
     2161 tcctcacgct ctcggcgctc gcgctgtcgc acatcctgta cgcgtacatg gcgatcatca
     2221 ccatgggggt cgtcttgctc gtgggcatca gtcgcgccaa cgcgctcgcg cgggcggcgc
     2281 gtctcgcgat cgtcggcacc gcgaccgcgg tcatcacctc gtacatgtgg atcccgacga
     2341 tccaatcggc cggcctctat ctcgacttca gcccctacct cgagcgatgg aagtacgact
     2401 cctttggcgc cgcgcgcgtg ctcgagtact tcgcgaaggg acagctgttc gatgagggcc
     2461 ggccgggcgt gctgagcgcg ctcgtggtcg ccgggctcct cgccgcggcc gcactgcgca
     2521 cgccggccgc gatcaccgcc atcgcgctct tcgcaacctg gctcgtgctc ttcttcggac
     2581 ggccgaccct cggccccctg gctgcgctct tcccactgaa cgatgcgctg tacttccacc
     2641 gcttcatcgg cggcgtccac ctcgccgcga tcgtcctcat cggtatcgcg ggtgggtacg
     2701 ccctggaccg cgcgcgcggg acgccggcgc gcgtggcggt gagcgtgctc ctcatcgcgc
     2761 tgctcctccc ggccttcgct gagcgcgcct cctattacgc gacacagacc ggatggatgc
     2821 gcacgacagc cgacgcgatc gacgccgacg cggacctccg cctcgtgctc accacgctct
     2881 cggcgctccc gccgggacgg gtgttcgcgg ggaagaacga cgactgggga ccccagctga
     2941 acttcggcgt gccgttcaga tcgacgcagg tccgccacat gctgaccttc accggcatac
     3001 cggaagcatc gatcccctac tccgggccat cgctgaacgc ggatctgctc tacgacttcg
     3061 acgaacggcg cgcggagctg tacgacctgt tcgacgcgcg ctacgtcatc gcgccgaccg
     3121 gtcgtgccat g
//
LOCUS       DASTZR010000089         3567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1180359, whole genome shotgun sequence.
ACCESSION   DASTZR010000089 DASTZR010000000
VERSION     DASTZR010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180359"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..330
                     /gene="rpsJ"
                     /locus_tag="VFV20_02020"
     CDS             22..330
                     /gene="rpsJ"
                     /locus_tag="VFV20_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU5287152.1"
                     /translation="MAQQKIRIRLKGYDHQQVDRSASQIVETAQRTGATITGPVPLPT
                     EKNVYCVIRGPFIDKDSREHFEVRTHKRLIDIHSPTPKTVDSLMRLDLPAGVDIEIKL
                     "
     gene            327..950
                     /gene="rplC"
                     /locus_tag="VFV20_02025"
     CDS             327..950
                     /gene="rplC"
                     /locus_tag="VFV20_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU5287153.1"
                     /translation="MMKGILGRKLGMTQVFDPETGNVDPVTVIQAGPCPVVTVRTQAA
                     DGYEAVQLAFEPVQDRKLSKGELGHLQKAGVGGHRHLVEFRGTSELQQGETVTVEAFE
                     PGDRVKVSGISIGKGFQGTIKRHNFSRGPMSHGSHNIRKPGSVGASATPSRVFKGKKM
                     AGQMGARRVTQPGLVVHEVDAERNLLLVRGSVPGPKSGLVEIRAVER"
     gene            1117..1749
                     /gene="rplD"
                     /locus_tag="VFV20_02030"
     CDS             1117..1749
                     /gene="rplD"
                     /locus_tag="VFV20_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU5287154.1"
                     /translation="MAVKATVLGGSAKDLVLDAEVFEAEVKPHLVHETVRAELNAARR
                     GTRGAKSRGLVSGGRSKPWRQKGTGRARQGTIRAPQFAGGGVAFPPSMRSFEVKVNRK
                     ARRAALRGALANHAANGTLAILDPQDFDAPSTRKAAQLLSEWQKDAPTLVVAHVEEEA
                     VVKSFRNLERVLVTVPEELEVAAIVWARALVVTQAALPQVEQRAGKEATP"
     gene            1755..2045
                     /gene="rplW"
                     /locus_tag="VFV20_02035"
     CDS             1755..2045
                     /gene="rplW"
                     /locus_tag="VFV20_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU5287155.1"
                     /translation="MTNEQILLSPVVSEKSYAGIAEGRYTFKVHPDAHRTQVRQAVEQ
                     LFDVHVERVNIVKVQAKPKRRGLFKGTRPGWKKAVVQVREGETIEIFEGAQV"
     gene            2058..2897
                     /gene="rplB"
                     /locus_tag="VFV20_02040"
     CDS             2058..2897
                     /gene="rplB"
                     /locus_tag="VFV20_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU5287156.1"
                     /translation="MPVKKYKPTSPGRRQMATSSFDEITRSKPEKSLLEPLHKTGGRN
                     GNGRITTRHQGGGHKRRYRRIDFKRAKDGVPAKVASIEYDPNRSARIALLHYADGAKA
                     YILAPAGLRVGATIQSGPGVDIRPGNALPLGDIPTGTNVHAVELRPGQGAKLARSAGS
                     GVQLVAKDAPYAVLRLPSGEMRRVLLTCRATVGQVGNSDHANESGGKAGRSRWLGRRP
                     TVRGTAMNPVDHPHGGGEGKSKGHHPVTPWGVPTIGKRTRPKHKESDRLIVRGRRRGK
                     ERR"
     gene            2904..3188
                     /gene="rpsS"
                     /locus_tag="VFV20_02045"
     CDS             2904..3188
                     /gene="rpsS"
                     /locus_tag="VFV20_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEU5287157.1"
                     /translation="MSRSSKKGPFVEERLMSRIESMNTAGEKRMIRTWSRASTIFPEM
                     VGHTIAVHDGRKHVPVFVSEQMVGHKLGEFAPTRTFRGHSGDRQAQVKKR"
     gene            3212..>3567
                     /gene="rplV"
                     /locus_tag="VFV20_02050"
     CDS             3212..>3567
                     /gene="rplV"
                     /locus_tag="VFV20_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013980754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEU5287158.1"
                     /translation="MQVRAQAKWVRMSARKARLVLDLVRGRTVPEARTVLAFTPRAAA
                     REIDRVLSSAVANAESAHGLDGDELVVVAAYADEGPTLKRWRARARGRVNRIRKRTCH
                     ITVVVAPVAERPRRDQG"
BASE COUNT          607 a         1245 c         1274 g          441 t
ORIGIN      
        1 ttcgagggaa cgcgcataca catggcacaa cagaagatca ggatcaggct caagggctac
       61 gaccaccagc aggtcgatcg gtccgcctcc cagatcgtgg agacggccca gcgcaccggc
      121 gcgacgatca ccggtcccgt acccctgccc accgagaaga acgtgtactg cgtgatccgc
      181 gggccgttca tcgacaagga ctcgcgcgag cacttcgagg tgcggaccca caagcgcctg
      241 atcgacatcc acagccccac gccgaagacg gtcgactcgc tcatgcgcct cgacctcccg
      301 gcgggcgtcg acatcgagat caagctgtga tgaagggcat cctcggcagg aagctcggca
      361 tgacgcaggt cttcgacccc gagaccggga acgtcgaccc cgtcacggtg atccaggcag
      421 gcccctgccc ggtggtgacc gtgcggacgc aggcggcgga cggctacgag gccgtccaac
      481 tcgcgttcga gccggtgcag gaccgcaagc tctcgaaagg agagctcggc cacctgcaga
      541 aggccggcgt cgggggccac cgccacctcg tcgagttccg cggcacgagc gagctccagc
      601 agggcgagac cgtgacggtc gaggcgttcg agccgggcga ccgcgtcaag gtgtcgggga
      661 tctcgatcgg caagggcttc cagggcacga tcaagcggca caacttcagc cgcggcccga
      721 tgagccacgg ctcgcacaac atccgcaagc cgggctcggt cggcgcctcg gccacgccgt
      781 cgcgcgtgtt caagggcaag aagatggccg gccagatggg cgcgcgtcgc gtaacccagc
      841 cggggctcgt cgtccacgag gtcgacgccg agcgcaacct gctgctcgtg cgcggctccg
      901 tgccgggccc caagagcggg ctcgtcgaga ttcgggcggt ggagcgttga acgcgctgcc
      961 ctccgtcgca tctcgcgcgc tggatgccgc tgcgaagcgg cgcctcgcgt cgtcctcggt
     1021 cgcgcccgta gcctctggct acgaacacta cctgcgtcct agcgaggctt gcttctcgcg
     1081 gccccagcgg gcgacctgca acggagggca gcgctgatgg ccgtcaaggc gaccgtgctc
     1141 ggcggctcgg ccaaggacct cgtgctcgac gcggaggtct tcgaggccga ggtcaagccg
     1201 cacctcgtgc acgagaccgt gcgcgcggag ctgaacgccg cgcggcgggg cacgcgtggc
     1261 gcgaagagcc gcggcctcgt ctccggcggc cgctccaagc cgtggcgcca gaagggcacg
     1321 ggccgcgccc gccagggcac gatccgcgca ccgcagttcg cgggcggcgg cgttgcgttc
     1381 ccgccgagca tgcggagctt cgaggtgaag gtgaaccgca aggcgcggag ggccgcgcta
     1441 cgcggcgcgc tcgcgaatca tgctgcgaac gggacgcttg cgatcctgga tccgcaggac
     1501 ttcgatgcgc cctccactcg gaaggcagcg cagctgcttt ccgagtggca gaaggatgcg
     1561 ccgacgctcg tcgtcgccca cgtcgaggag gaggcggtcg tgaagtcgtt ccgcaatctc
     1621 gagcgggtgc tcgtcaccgt ccccgaggag ctcgaggtgg ccgcgatcgt ctgggcccgg
     1681 gcgctcgtcg tgacccaggc cgcgctgccg caggtcgagc agcgcgccgg caaggaggcg
     1741 acgccgtgac ggcgctgacc aacgagcaga tcctcctctc gcccgtggtc tcggagaaga
     1801 gctacgcggg aatcgcggag ggccggtata cgttcaaggt gcatcccgac gcgcaccgca
     1861 cccaggtgcg ccaggcggtg gagcagctgt tcgacgtcca cgtcgagcgg gtgaacatcg
     1921 tgaaggtgca ggcgaagccc aagcgccgtg ggctgttcaa gggcacacgg ccggggtgga
     1981 agaaggcggt cgtccaggtc cgtgagggcg agacgatcga gatcttcgaa ggcgcgcagg
     2041 tctaggagaa cacgcagatg ccagtcaaga aatacaagcc gacgagcccg ggccgccgcc
     2101 agatggcgac ctcgtcgttc gacgagatca cgcgctcgaa gcctgagaag tcgctgctcg
     2161 agccgctcca caagaccggc ggccgcaacg gcaacgggcg gatcaccacg cgccaccagg
     2221 gcggcggcca caagcgccgc taccggcgca tcgacttcaa gcgcgcgaag gacggcgtgc
     2281 cggccaaggt cgcgtcgatc gagtacgacc cgaaccgctc cgcgcggatc gcgctgctgc
     2341 actacgccga cggcgcgaag gcgtacatcc tcgcgcccgc gggcttgcgc gtcggggcga
     2401 ccatccagtc gggccccggc gtcgacatcc ggcccggcaa tgcgctgccc ctcggcgaca
     2461 tcccgacggg tacgaacgtc cacgccgtcg agctgcggcc gggccagggc gcgaagctcg
     2521 cccgttcggc aggctcgggc gtgcagctcg tcgcgaagga cgcgccgtac gccgtgctgc
     2581 gcctcccctc gggcgagatg cgccgcgtac tgctcacctg ccgcgcgacc gtcggccagg
     2641 tcgggaactc cgaccacgcg aacgagtccg gcgggaaggc cggtcggagc cgctggctcg
     2701 gccgccggcc gaccgtccgc ggcaccgcga tgaatccggt cgaccacccg cacggtggcg
     2761 gcgaaggcaa gtccaagggc caccacccag tgaccccgtg gggcgtcccg acgatcggga
     2821 agcgcacccg tcccaagcac aaggaatccg acaggctgat cgtccgcggc cgccgccgcg
     2881 gcaaggaaag gcgctaacca gatatgagca gatcgtccaa gaaggggccg ttcgtcgagg
     2941 agcggctgat gagtcgcatc gagtcgatga acaccgccgg cgagaagcgg atgatccgca
     3001 cctggtcgcg cgcgtcgacg atcttccccg agatggtcgg gcacacgatc gccgtgcacg
     3061 acgggcggaa gcacgtgccc gtgttcgtga gcgagcagat ggtcggccac aagctcggcg
     3121 agttcgcgcc gacgcggacg ttccgggggc actccggcga ccgccaggcg caggtgaaga
     3181 agcgctgatg gcgacgaaga cgaacacgga gatgcaggtc cgcgcgcagg cgaagtgggt
     3241 gcgcatgtcc gcgcgcaagg cgcggctcgt gctcgacctc gtccgcggcc gcaccgtgcc
     3301 cgaggcgcga accgtgctcg cgttcacgcc gcgcgcggca gcgcgcgaga tcgaccgcgt
     3361 gctctcgtcg gccgtcgcga acgccgagtc ggcgcacggc ctcgacggcg acgagctcgt
     3421 cgtggtcgcc gcctacgccg acgaggggcc gacgctcaag cgctggcgcg cccgcgcgcg
     3481 cggccgcgtc aaccgcatcc gcaagcgcac gtgccacatc accgtcgtcg tcgcgccggt
     3541 ggccgagcgg ccgcgccgcg accaggg
//
LOCUS       DASTZR010000090         5048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1205536, whole genome shotgun sequence.
ACCESSION   DASTZR010000090 DASTZR010000000
VERSION     DASTZR010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5048
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205536"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /locus_tag="VFV20_02055"
     CDS             <1..1126
                     /locus_tag="VFV20_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287159.1"
                     /translation="QALRRPLRTGGAAATRGMTTIVDLEEQIAGLPAEARAAASRIFT
                     VSTTTGVLDAPPEMHAWIAKLFGSVDAVREQRIVRVTNTVTFEGALFNELRAMRPMEV
                     KGGDEVRQTIESARGDPFDHPETGTPADSFGRIAGKHGLTASNIAKYDGFHGVLVFKE
                     HDPLAPMGPAVLRDHLATARRWGEAALASDPAARYLFVMWNCLWRAGGSIVHGHMQMT
                     ATRVAHYPKVEALRRQALAYATSTGHDYFDDLWLVHSALGLGVEVDGARVLASLVPVK
                     EREVLVIGRPGADESSLAPAIVRVLASYRASGVVAYNMGLYLPPLSPDGEDWRRFPPI
                     ARLVDRGDPANKTSDIGAMELYAASVIASDPFRLADQLRT"
     gene            1248..1700
                     /locus_tag="VFV20_02060"
     CDS             1248..1700
                     /locus_tag="VFV20_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEU5287160.1"
                     /translation="MADLTFVSRRDSSFKEFPPASAQIAMVVPFYGELTVWVRSPDAG
                     GWHFPTSARRSGETILEVARRELWDSARIVASRLDLLGAIRYELPKPTTAYVYMCELQ
                     HLTWWYDIPEKEKVAEVGVFARLPRPVASEAVELVLEAALRARRTGLR"
     gene            complement(1987..2715)
                     /locus_tag="VFV20_02065"
     CDS             complement(1987..2715)
                     /locus_tag="VFV20_02065"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU5287161.1"
                     /translation="MRVAVAILVIAASAVAAFALAPAPRDTPAPVTTPTAAPFVTADP
                     SVTARADPVAVGGRSGEDDRAVRRAVRVRGLLLPVDGVELPTDDELLPNAERAYRGGW
                     HEGIDFPAPAGTPVRAVARGVVVRIDREFTDWPAESERAALAEATRLGYTPDVTLDLI
                     RGRQVWIDHGGGIVSRYAHLDTVADLAVGARVERGTIVGTVGSSGYPEGGPHLHLEIR
                     TGTSYLGDGLTGDALRAAVAAAFG"
     gene            2840..3415
                     /gene="pyrR"
                     /locus_tag="VFV20_02070"
     CDS             2840..3415
                     /gene="pyrR"
                     /locus_tag="VFV20_02070"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEU5287162.1"
                     /translation="MAQPRSVLLSADDVRRAVSRIAHEIVERDHDLSTIAIVGIRSRG
                     DVLAVRLRESLREHEHASVPLGSLDITLYRDDLTRIGYAPVVHESAIGFSVDDKVVVL
                     VDDVLFTGRTIRAALDALVDYGRPRAIRLAVLVDRGHRELPIRADFVGKNVPTQLTDD
                     VRVHLTEVDGRDEVELVARGAAVEATERSRA"
     gene            3412..4383
                     /locus_tag="VFV20_02075"
     CDS             3412..4383
                     /locus_tag="VFV20_02075"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEU5287163.1"
                     /translation="MTLIQERTARWTRRHVLDTDDWSRGEIEAVLETTGAMNEVLSRP
                     IRKAPPLRGKTVILFFAEASTRTRVSFELAAKALSADVTNITASGSSVEKGETLFDTV
                     RTLQALGGDIVVMRHASSGAPYFVAEHTDAPVINAGDGWHAHPTQGLLDAYTLRGALG
                     DLAGKRIVIVGDVLHSRVARSDINTLVPLGARVTLSGPPTLMPAAWRCGALPTAVSYE
                     PDLDRAIDGADAVIALRLQKERQESGLLPSLREYTRVWGLTEARVARMRPEAPVLHPG
                     PMNEGIEIDAAVAHGARSLVERQVTNGVAVRMALLYLLVGTPSLEER"
     gene            4383..>5048
                     /locus_tag="VFV20_02080"
     CDS             4383..>5048
                     /locus_tag="VFV20_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5287164.1"
                     /translation="MDRRDYERQGYLVANGFIDLHAHLREPGFEDSETVATGARAALA
                     GGFTTVCAMPNTEPAIDSPGLVAQLIARGAAANGARVLPIAAITRGRKGKELADLVEL
                     AAAGAVAFSDDGSPVEDARLFRHALEYAQARDLLVIEHPQDLSLSAKGVMHEGTMSAR
                     LGLPGVPAAAEENAVARDLALAQLTGARLHLTHLSTRGAVELVRQAKAGGLAVTADVT
                     PHHL"
BASE COUNT          728 a         1838 c         1746 g          736 t
ORIGIN      
        1 ccaggcgcta cgtcgacctc tacgcacggg cggcgcggct gcgacgcgtg gcatgacgac
       61 gatcgtcgac ctcgaggagc agatcgcggg tctgccggcc gaggcgcgcg cggcggcatc
      121 ccgcatcttc accgtctcga ccacgaccgg ggtgctggac gccccgcccg agatgcacgc
      181 gtggatcgcc aagctgttcg gctccgtgga cgccgtgcgc gagcagcgca tcgtccgggt
      241 gacgaacacg gtgacgttcg agggcgcgct cttcaacgag ctccgcgcga tgcggccgat
      301 ggaagtgaag ggcggcgacg aggtgcggca gaccatcgag tccgcgcggg gcgacccgtt
      361 cgaccatccc gagaccggta cgccagcgga cagcttcggc cgcatcgcgg gtaagcatgg
      421 gctcaccgcc tcgaacatcg cgaagtacga cggtttccac ggcgtgctcg tcttcaagga
      481 gcacgacccg ctcgcgccga tgggtcctgc ggtcctccgc gaccacctcg cgaccgcgcg
      541 tcgctggggc gaggcggcgc tcgcgtccga tccggcggcg cgctacctct tcgtgatgtg
      601 gaattgcctc tggcgcgccg gggggtcgat cgtgcacggg cacatgcaga tgaccgccac
      661 gcgcgttgcc cactatccga aggtcgaggc gctgcgccgg caggcgctcg cctacgccac
      721 atcgacgggc catgactact tcgacgacct ctggctcgtc cacagcgcgc tgggactcgg
      781 ggtcgaggtc gacggcgcgc gggtgctcgc ctcgctggtc ccggtgaagg agcgtgaggt
      841 cctggtcatc ggacgacccg gcgcagatga gagctcgctc gcgcccgcca tcgttcgcgt
      901 gctcgccagc taccgcgcca gcggcgtcgt cgcatacaac atggggcttt acctgccgcc
      961 cctctcgccc gacggcgagg actggcgccg gttcccgccc atcgcacgac tcgtcgatcg
     1021 cggcgatccc gcgaacaaga cctcggacat cggcgcgatg gagctctatg ccgcgtcggt
     1081 catcgcgagc gacccgttcc gcctcgcgga ccagcttcgc acctgatcgg tcaccatccg
     1141 gctcgcgcga acgtctatat tcagtcgtcc gtctgctcgc tttgactagc ttgccaccac
     1201 acggcctatt gacgagtcgc tactcgtccg gcttactgag caccacgatg gcggatctga
     1261 cattcgtctc acgacgagac tccagcttca aggagttccc gccagcgtcc gcccagatcg
     1321 cgatggtcgt ccccttctac ggagagctca cggtgtgggt ccgctcgccc gacgcgggcg
     1381 ggtggcattt tccgacgagc gcccgccgct caggggaaac gatcctcgag gtcgcgcgtc
     1441 gcgagctctg ggattcggcg cgcatcgtcg cgagccgcct cgatcttctc ggcgcgatcc
     1501 gttacgagct gccgaagccg acgaccgcct acgtgtacat gtgcgagctc cagcacctga
     1561 cctggtggta cgacatcccc gagaaggaga aggtcgcgga ggtcggcgtg ttcgcgcggc
     1621 tcccgcgccc ggtcgcttcc gaggcggtcg agctggtgct cgaggccgcg cttcgcgcgc
     1681 gccgtaccgg actccgctaa ggtcgcgccg cagcgcgatc gcgtaacaac cccgaacctg
     1741 taccggacct gagcctccgc tggcaagcca cttgcgcgtt cgcttaagcg tgatcgacga
     1801 agagaccgag cgagcggtcg ctggttgggt acgtctcggg gaccggatgg ccggccagcg
     1861 tccggccgac gaccggacgc gctgtgcccg ctgcggctcg agtcaccggg tgctgggcca
     1921 cggcaccaat gccctctgcg cccactgcta cctcgaacgc ggcggccgcg acctggccac
     1981 cgtctcctag ccgaacgccg ccgcgacggc ggcgcggagc gcgtcccccg tgagcccgtc
     2041 acccaggtag ctcgtccccg tccggatctc gaggtgcaga tgcggcccgc cttcggggta
     2101 cccgctcgat ccgacggtcc cgacgatcgt ccctcgctcc acacgcgctc cgaccgcgag
     2161 atcggcgacg gtgtcgaggt gcgcgtagcg gctcacgatc ccgcccccgt ggtcgatcca
     2221 cacctggcga ccgcggatca gatcgagcgt cacgtccggc gtgtagccca ggcgcgtcgc
     2281 ctcggcgagc gcggcccgct cgctctcggc gggccagtcg gtgaattcgc ggtcgatgcg
     2341 cacgacgacg cctcgggcga cggcacgcac cggcgtgccg gcgggcgcgg ggaagtcgat
     2401 gccctcgtgc caacccccgc ggtacgcgcg ctcggcgttc ggcaggagct cgtcgtcggt
     2461 cggaagctcc acgccatcca cgggaaggag caggccgcgg acgcggaccg cacgtcgaac
     2521 ggcgcggtcg tcctcgccgg agcgcccgcc gaccgcgacc ggatcggcgc gcgccgtgac
     2581 actcgggtcg gcggtcacga acggggcggc cgtcggcgtg gtcaccggtg ccggcgtgtc
     2641 gcgcggcgcg ggcgcgagcg cgaacgccgc caccgcggac gcggcgatga ccaggatcgc
     2701 gacagccacc cgcacgggta caccgacggt accgcaggtg caggtggccg ccggcgatga
     2761 ccgcctgcac catccgctag acttcgatgc agcacgacct ttaacgaccg gtcctgagag
     2821 gccggaaagg gagtggcaga tggcccagcc gcgttccgtc ctgctcagtg ccgacgacgt
     2881 ccgtcgtgcg gtctcccgca tcgctcacga gatcgtcgag cgcgatcacg acctctccac
     2941 catcgccatc gtcggcatcc gctcgcgcgg cgacgtgctc gccgtgcgtc tgcgcgagtc
     3001 gctccgcgag cacgagcacg cgagcgtgcc cctcggttcc ctcgacatca cgctgtaccg
     3061 cgacgacctc acgcgcatcg ggtacgcgcc ggtggtgcac gagtcggcga tcggcttctc
     3121 cgtcgacgac aaggtcgtcg tgctcgtgga tgacgtgctc ttcaccggcc gcacgatccg
     3181 cgcggcgctc gacgcgctcg tcgattacgg gcggccgcgc gcgatccgcc tcgcggtgct
     3241 cgtggaccgc gggcaccgcg agctgccgat ccgcgcggac ttcgtcggaa agaacgtgcc
     3301 cacccagctc accgacgacg tgcgcgtgca tctcaccgag gtcgatggtc gtgatgaggt
     3361 cgagctcgtc gcacgcggtg cggcggtcga ggcgaccgaa aggagccgtg catgaccctc
     3421 atccaggaac gcacagcgcg atggacacgc cgccacgtgc tcgacacaga cgactggtca
     3481 cggggtgaga tcgaggccgt cctcgaaacg accggcgcga tgaacgaggt gctgtcgcgc
     3541 ccgatccgca aggcgccgcc actgcgcggc aagacggtca tcctcttctt tgcggaggcg
     3601 tcgacgcgca cccgggtgtc gttcgagctc gccgcgaagg ccctctcggc cgacgtcacg
     3661 aacatcaccg cgagcggctc ctcggtggag aagggcgaga cgctcttcga caccgtgcgc
     3721 accctgcagg cactgggcgg cgacatcgtg gtcatgcgcc atgcctcgtc cggcgcgccg
     3781 tacttcgtcg cggagcacac cgacgcgccg gtgatcaacg ccggcgatgg ctggcacgcc
     3841 catcccacgc agggcctgct ggacgcgtac acgctccgcg gcgcgctcgg tgatctcgcc
     3901 ggcaagcgca tcgtcatcgt cggcgacgtc ctgcacagcc gtgtggcgcg ctccgacatc
     3961 aacacgctcg tcccgctcgg agcgcgggtg acgctgtcgg gaccgccgac gctcatgccc
     4021 gccgcctggc gctgcggcgc gcttcccacg gcggtctcgt acgagccgga tctcgatcgt
     4081 gccatcgacg gggccgacgc cgtgatcgcg ctgcgcctcc agaaggagcg ccaggagagc
     4141 ggactccttc catcgctgcg cgagtacacg cgcgtgtggg gcctcaccga ggcgcgcgtg
     4201 gcgcggatgc gccccgaggc gccggtgctg catccggggc ccatgaacga aggcatcgag
     4261 atcgacgcgg ccgtggcgca cggcgcgcga tcgctggtcg agcgacaggt gacgaacggc
     4321 gtggcggtgc ggatggcgct cctctatctg ctcgtcggga cgccgtcgct ggaggaacgc
     4381 tgatggaccg ccgcgactac gagcgccagg gctatctcgt cgcgaacggc ttcatcgacc
     4441 tccacgcgca tctgcgcgag cccggcttcg aggattccga gacggtggcg acgggcgcgc
     4501 gcgccgcgct cgccggtggc ttcacgacgg tctgcgccat gccgaacacc gagccggcca
     4561 tcgattcgcc cgggctcgtc gcgcagctga tcgcgcgcgg cgcggcggcg aacggcgcgc
     4621 gcgtgctgcc catcgcggcg atcacgcgcg gccgaaaggg gaaggagctc gccgatctgg
     4681 tcgagctcgc ggcggccggt gcggtcgcgt tctcggacga cggctcgccg gtcgaagacg
     4741 cgcgtctctt ccggcacgcg ctcgagtacg cgcaggcgcg cgatctcctg gtcatcgaac
     4801 acccgcagga cctgtcgctc tcggcgaaag gcgtgatgca cgagggcacg atgtccgcgc
     4861 gcctcggcct ccccggcgtg ccggccgcgg ccgaggagaa cgcggtcgcg cgggatctcg
     4921 cgctcgcgca gctcacgggc gccaggctcc acctcacgca cctctcgacc cgcggcgcgg
     4981 tcgagctcgt gcgccaggcg aaggcgggcg gcctcgccgt gacggcggac gtcacgcccc
     5041 accacctc
//
LOCUS       DASTZR010000091         2074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_240519, whole genome shotgun sequence.
ACCESSION   DASTZR010000091 DASTZR010000000
VERSION     DASTZR010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240519"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VFV20_02085"
     CDS             complement(<1..443)
                     /locus_tag="VFV20_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5287165.1"
                     /translation="MKLAAHLWPQNTTWEAIRDAAVVADRVGFEMVTIWDHFYALQGE
                     EERPNLETWTILSGLAAVTSRVRLASLVQSVTYRHPAIVANIAATHDQISQGRTICGI
                     GAGWNQTEHRAYGIELGTPGERSRRLAEAAKVIRALLDGERVTYDG"
     gene            499..1848
                     /locus_tag="VFV20_02090"
     CDS             499..1848
                     /locus_tag="VFV20_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020519264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5287166.1"
                     /translation="MAVETKGSVDAARVAKETKEHVLVSWSAQGALAPLVITRAEGSW
                     LHAGDRKILDFSSGLINVNLGHGHPKVVKAIQEQAEKLCYVTPSFGEESRAELARLLS
                     EVAPGHLAKTLFTTGGTEAVEHAVRIARQFSGRHKILSQWRSFHGQTAGSATVGGDNR
                     RWANEPGITGVVHFLNPDPYRSLFGGDAQKALAHVEEVIWYEGPQYIAAILLEPIVGS
                     SGLIVPPDGFLRGLRQLCDQHGILLVLDEVMTGFGRTGKWFAAEHEGVVPDMMTFAKG
                     VNSGYVPLGGVMVSDRIAKHFDTNVLWSGLTYSGHPLACAAGVATVNAYRDERIIEHG
                     AKMGELLMKELRTIATKHPSVGDVRGRGPFIGIELVKDRGTKEMLERWNGPSTKLQNA
                     LKNALMSRDVYLLNRWNMLFIAPPLTVSEDEVRFGVRAIDEALDIADRFAATGQLPA"
     gene            complement(1857..>2074)
                     /locus_tag="VFV20_02095"
     CDS             complement(1857..>2074)
                     /locus_tag="VFV20_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287167.1"
                     /translation="GKFEMELFIALGAIVALDVLALEFGFGGPQDRAAHHHDQALEAI
                     KRGDVARYREEIARLESDIARDAWRLF"
BASE COUNT          327 a          724 c          706 g          317 t
ORIGIN      
        1 ccgtcgtacg tcacgcgctc gccgtcgagg agcgcgcgga tcacctttgc ggcctcggcc
       61 agacgccgcg agcgctcgcc gggcgtgccc agctcgatcc cgtacgcgcg atgctcggtc
      121 tggttccagc ccgcgccgat cccgcagatc gtccggccct gcgagatctg atcgtgcgtc
      181 gccgcgatgt tcgcgacgat cgcggggtgc cgataggtca cgctctgtac gagcgatgcg
      241 agccgcacgc gcgacgtgac cgcggcgaga ccggagagga tcgtccacgt ctcgaggttc
      301 ggccgctcct cctcgccttg cagcgcgtag aagtggtccc agatcgtgac catctcgaac
      361 ccgacgcggt cggcgacgac ggcggcgtcg cggatcgcct cccaggtggt gttctgcggc
      421 cagaggtgtg cggcgagctt cacgcgtcga gggtatcgcc cggcggtgga ccccgcgtga
      481 gcgacgtaca ctcggcacgt ggccgtcgag acgaagggaa gcgtcgacgc cgcccgcgtc
      541 gccaaggaga cgaaggagca tgtgctcgtc tcgtggtccg cgcagggcgc gctcgcgccg
      601 ctcgtcatca ccagggcaga gggctcctgg ctccatgccg gcgaccgcaa gatcctcgac
      661 ttctcgtccg gtctgatcaa cgtcaacctc ggccatggac atccgaaggt cgtgaaggcg
      721 atccaggagc aggccgagaa gctctgctac gtcaccccat cgttcggcga ggagagccgc
      781 gcggagctcg cgcgcctcct gtccgaggtc gcgccgggcc acctcgcgaa gacgctcttc
      841 accaccggcg gcaccgaggc cgtcgagcat gcggtccgca tcgcgcgcca gttcagcggt
      901 cggcacaaga tcctgtcgca gtggcggtcc ttccacgggc agaccgcggg gtccgccacc
      961 gtcggcggcg acaaccgccg ctgggcgaac gagccgggca tcaccggggt cgtccacttc
     1021 ctcaaccccg atccctaccg ctcgctgttc ggcggcgacg cgcagaaggc gctcgcgcac
     1081 gtcgaagagg tcatctggta cgaggggccg cagtacatcg cggcgatcct gctcgagccg
     1141 atcgtcggat cgagcggcct catcgtgccg cccgacggat tcctccgtgg gctccggcag
     1201 ctctgcgacc agcacggcat cctgctcgtc ctcgacgagg tcatgacggg cttcgggcgg
     1261 acggggaagt ggttcgctgc ggagcacgag ggcgtggtgc cggacatgat gaccttcgcg
     1321 aagggcgtga actccggcta cgttcccctc ggcggcgtca tggtgagcga ccgcatcgcc
     1381 aagcacttcg acacgaacgt gctgtggtcc ggcctcacgt actccggcca tccgctcgcg
     1441 tgcgcggccg gcgtggcgac ggtgaacgcc taccgcgacg agcggatcat cgagcacggc
     1501 gcgaagatgg gggagctcct catgaaggag ctgcgcacca tcgcgacgaa gcacccgtcc
     1561 gtcggcgacg tgcggggcag gggaccgttc atcggcatcg agctcgtgaa ggaccgcggg
     1621 acgaaggaga tgctcgagcg ctggaacggg ccgtcgacga agctgcagaa cgcgctcaag
     1681 aacgcgctca tgtcacgcga cgtctatctc ctgaaccggt ggaacatgct cttcatcgcg
     1741 ccgccgctca ccgtcagcga ggacgaggtc cgcttcggcg tgcgggccat cgacgaggcg
     1801 ctcgacatcg cggaccggtt cgccgccacc ggccagctgc ccgcttagga gcccgtctag
     1861 aacagacgcc acgcgtccct cgcgatgtcg ctctccagac gcgcgatctc ctctcggtac
     1921 cgtgcgacgt cgccgcgctt gatggcctcc agcgcctggt cgtgatggtg agcggcgcgg
     1981 tcctgcgggc cgccgaagcc gaactcgagc gccagtacat cgagcgcgac gatcgcgccg
     2041 agcgcgatga acagctccat ctcgaacttc cctt
//
LOCUS       DASTZR010000092         2375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_787972, whole genome shotgun sequence.
ACCESSION   DASTZR010000092 DASTZR010000000
VERSION     DASTZR010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787972"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..524)
                     /locus_tag="VFV20_02100"
     CDS             complement(21..524)
                     /locus_tag="VFV20_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287168.1"
                     /translation="MIVGGYDVAAVPEISYGLQRGGVARVLSRLAMRSAARVVPFSAA
                     ALRETRENAGIPERAIALIPLGVADAGPVPTGPRDIVLTVARVDAENLHRKGLLDVAR
                     TARLLTCETVIAGEIADERVAQTLREVAGPMLRLVGRVTDAELLALQARAFAYLQLSR
                     HDGFGLA"
     gene            complement(758..949)
                     /locus_tag="VFV20_02105"
     CDS             complement(758..949)
                     /locus_tag="VFV20_02105"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEU5287169.1"
                     /translation="MTRLVCNALMRIDARLLADLVCPVDHSAVREDGEDLTCMSCGRR
                     YPVRDGIPVMLESDAERHG"
     gene            complement(930..>2375)
                     /locus_tag="VFV20_02110"
     CDS             complement(930..>2375)
                     /locus_tag="VFV20_02110"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU5287170.1"
                     /translation="AAFGGARGKEGAIIVGAGRTGALLARDLSDGSRGTRIIAFVDDD
                     PRKHGTYVRGARVEGGVDDLPALISRTKPAVVVIAVAEASGDLVRRVLTATRNTSVRV
                     RAVGGFRLGASDRGPLRAIDIDELLQREPVHLDAPDTRAFLEGRRVLVTGAAGSIGAE
                     ISRRVLEVAPSEVVLLDANESGLHDVVAALAPDGGVRLELGDVRDAAALRRTIAEVAP
                     DVVFHAAAYKHVPILERAPLPGIVTNVLGTARLLGALDAAAVDSFVFISSDKAVAPAS
                     VLGLTKRFGELLTFAYARAHGRRWSVVRFGNVLGSSGSVVPIFTRQIDAGGPITVTHP
                     DVTRYFMTIPEAVGLVIRAASVAEPGDLLLLDMGEPVRIVDLARQMIWLRGLRTPQDI
                     EIIYTGLRPGEKLSEDLWLPSERPIPTAHARVLRASAAADAPPLAELLEITARVAAAV
                     ADGDETRAIAELRDAVGVPIDSAGGHDPARV"
BASE COUNT          406 a          817 c          824 g          328 t
ORIGIN      
        1 cccgcgcgca gtgcctccgc ctacgcaaga ccgaacccgt catggcgtga cagctggagg
       61 taggcgaagg cgcgggcctg aagggcgagc agttcggcat cggtcacccg gccgacgagt
      121 ctgagcatgg gaccggccac ctcgcgcagt gtctgggcga cccgctcgtc ggcgatctca
      181 cctgcgatca ccgtctcgca ggtcagcagt cgcgccgttc gcgcgacgtc gagcagaccc
      241 ttgcggtgca gattctcggc atcgacgcgg gcgacggtca gcacgatgtc gcgcggcccg
      301 gtcgggaccg gccccgcgtc ggcgacgcca agcggaatga gcgcgatcgc tcgctcgggg
      361 atcccggcgt tctctcgcgt ctcacgcagc gcagcagccg agaacggcac cacccgcgcg
      421 gccgatcgca tcgcaagacg ggagagcact cgcgcgacgc ccccgcgttg gagtccatac
      481 gagatctcgg gcacggcagc gacgtcgtag ccgccgacga tcacgatgcc cggacgacgc
      541 aggacgcgcg ccagaaggag aggcagcacg gcgtgccacg acgcgaacca gcacagcacg
      601 atgtcggcgc gcgcgaccgc cgggacgagc ttccagggcg cggggtccca tcgcctgcgg
      661 gcaaagagca cgtcaacgtc gaatagctgc gcggcgatct cgatgtcttg ctgaacgaat
      721 gtcgcaagac ctctatgaac gatgaggaga cgggggctca tccatgccgc tcagcatcgc
      781 tctccagcat caccgggatc ccgtctcgga ccggataacg ccggccgcag gacatgcatg
      841 tcaaatcttc cccgtcctcc cggacggccg aatggtccac cggacagaca aggtctgcga
      901 gaaggcgcgc atcgatcctc atcagggcat tacacacgag ccgggtcatg gccgccagcc
      961 gaatcgatgg gaacgccgac cgcgtcgcgc agctctgcta tcgcccttgt ctcatcacca
     1021 tctgctaccg cggcggcgac gcgtgcggtg atctcgagga gctcggcgag cggcggagca
     1081 tcggcggcgg cggaggcgcg cagcactcgc gcgtgagcgg tggggatggg acgttcgctc
     1141 gggagccaga ggtcttcgct cagcttctcg ccgggacgca gtccggtgta gatgatctcg
     1201 atgtcctgcg gcgttcgcag gccgcgcagc cagatcatct gcctcgcgag gtcgacgatg
     1261 cgcaccggct cgcccatgtc cagaagcagg aggtcgccgg gttcggccac cgatgccgct
     1321 cggatgacga ggccgaccgc ttcggggatc gtcatgaagt agcgcgtcac gtccgggtgc
     1381 gtgacggtga tcggaccacc cgcgtcgatc tgtcgcgtga agatcggtac gacgctgccg
     1441 gacgagccga gcacgttccc gaatcgcacg accgaccagc gtctcccgtg tgcgcgcgcg
     1501 tatgcgaagg tgagcagctc accaaagcgc ttcgtgagcc ccagaacgct cgcgggcgcg
     1561 accgccttgt ccgacgagat gaacacgaac gaatcgacgg cggccgcgtc cagtgctccg
     1621 aggaggcgtg cggtcccaag gacgttcgtc acgatgcccg gaagcggtgc gcgctcgagg
     1681 atcgggacgt gcttgtacgc ggccgcgtgg aacacgacgt cgggcgcgac ctcagcgatg
     1741 gtgcgtctca acgcggcagc gtcgcggacg tcgccgagtt cgagacgtac gcccccatcc
     1801 ggcgcgagcg ctgcgacgac gtcgtgcaga ccgctctcgt tcgcgtcgag cagcacgacc
     1861 tcggacggcg cgacctccag cacgcggcgc gagatctcgg cgccgatcga gcccgcggcg
     1921 ccggtgacga gcacccgtcg accctcgagg aatgcgcggg tgtcgggcgc gtcgagatgg
     1981 acgggctccc gctggagcag ttcgtcgatg tcgatggccc ggagcggacc acggtccgag
     2041 gctccgagcc ggaatccgcc caccgcgcga acgcggacgc tggtattgcg cgtggctgtg
     2101 aggacacgac gcaccagatc acccgaggcc tcagcgacgg cgatcacgac gacggcgggc
     2161 ttcgtgcggg agatcagcgc gggaagatcg tcgacgccac cttcgaccct cgcgccacgc
     2221 acgtacgtgc catgcttccg cgggtcatcg tcgacgaagg cgatgatgcg cgtgccgcga
     2281 ctgccgtcgg agagatcgcg ggcgagcagc gcccccgtcc gtccggcacc cacgatgatc
     2341 gcgccctcct tgccgcgggc gccgccgaac gccgc
//
LOCUS       DASTZR010000093         2196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_580311, whole genome shotgun sequence.
ACCESSION   DASTZR010000093 DASTZR010000000
VERSION     DASTZR010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580311"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VFV20_02115"
     CDS             <1..864
                     /locus_tag="VFV20_02115"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HEU5287171.1"
                     /translation="DIPFIRSTDGEASPQDIAYVVKQLEELIAQLENITGKSLDWKKL
                     VRTVELSAHVGEMWSEIKSLTKLRPAPYDAYFDSVTMMAVLYCLRGTPEAETFFEMAL
                     ADLKERSARKQGPLPEERFRVVIEGPPPWPFLRVFRDMFSRWGAVAVASTYSTVGGIW
                     EFGFAHDPQHPLESIAKHMLSQNLCNRTFLQRYDQMARYVDEWDADAVVIHSVKSCRL
                     FSAGQGDMREYFVREKEVPTLLIESDLEDPRYFSQAQLRNRIDAFFEALTQRRITAAA
                     DVAAAAAGGVR"
     gene            873..1661
                     /locus_tag="VFV20_02120"
     CDS             873..1661
                     /locus_tag="VFV20_02120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HEU5287172.1"
                     /translation="MGVDVGSTQTKAVLVDEKGQIVARSLIDTGAYLVRAAERSLEAA
                     LAEAGVSRSDVGYAVGTGYGRFKVAFGDDQVTEISCHAKGAWHLFPKTRTVIDIGGQD
                     TKAIKVSDRGEVLDFAMNDKCAAGSGRFLTNSAEALGMDVAQIGAKSLEAKNPVRLST
                     VCTIFVETDILSYLASGKKTEDILAGVHGAIGSRTVALVRRVGAEPEITFTGGVARNI
                     GMIRSVQEKLDLNINVSPDSHYTGALGGAIFALERMTAGSQVLA"
     gene            1658..>2196
                     /locus_tag="VFV20_02125"
     CDS             1658..>2196
                     /locus_tag="VFV20_02125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HEU5287173.1"
                     /translation="MNVDGRLVAGIDVGARSAKAVILDEEHRVLGSGLARMRPDFTGL
                     ARDVLTSAIDQAGVTPDDVAYVATTGLGRYNIPFRDLQITDITAVARGASHLFPGTRC
                     VLDIGAQSTRALHVLESGRVKEFRTNDKCAAGAGGFAERAARYLEIKLDDLGPLSLKA
                     DAPQVISSVCAVFAESEII"
BASE COUNT          361 a          768 c          744 g          323 t
ORIGIN      
        1 gacatcccgt tcatccgctc gacggacggc gaggcgtcgc cgcaggacat cgcgtacgtc
       61 gtcaagcagc tcgaggagct catcgcacag ctcgagaaca tcaccgggaa gagcctcgac
      121 tggaagaagc tcgtgcgcac ggtcgagctc tccgcgcacg tcggcgagat gtggtcggag
      181 atcaagtcgc tgaccaagct ccgccccgcg ccgtacgacg cgtacttcga ctcggtcacg
      241 atgatggccg tcctgtactg cctgcgcggc acgccggagg ccgagacctt cttcgagatg
      301 gcgctcgccg atctgaagga gcgcagcgcg cgcaagcagg gcccactccc cgaggagcgc
      361 ttccgcgtcg tcatcgaggg accgccgccg tggccgttcc tccgcgtgtt ccgcgacatg
      421 ttcagccgct ggggcgcggt cgctgtcgcg tcgacgtact cgacggtcgg cgggatctgg
      481 gagttcggct tcgcgcacga tccccagcac ccgctggaga gcatcgccaa gcacatgctg
      541 tcgcagaacc tctgcaaccg gacgttcctg cagcgctacg accagatggc gcggtacgtg
      601 gacgagtggg acgccgacgc ggtcgtgatc cactcggtga agtcgtgccg cctcttctcc
      661 gctgggcagg gtgacatgcg cgagtacttc gtccgcgaga aggaggtccc gacgctcctc
      721 atcgagtcgg acctcgagga cccgcgctac ttctcgcagg cgcagctgcg caaccgcatc
      781 gacgcgttct tcgaagctct cacgcagcgg cgcatcacgg cagccgccga tgtcgccgcc
      841 gcggccgccg gaggtgtcag gtgaggtatg cggtgggcgt ggacgtcggg tccacgcaga
      901 cgaaggccgt cctggtggac gagaagggac agatcgtcgc ccggtcgctg atcgacacgg
      961 gcgcgtacct ggtgcgtgcc gcggagcggt cgctcgaggc cgcgctcgcg gaggccggtg
     1021 tgtcgcggag cgacgtcggc tacgcggtcg gcaccggcta cggccggttc aaggtggcgt
     1081 tcggcgacga ccaggtcacc gagatctcgt gccacgccaa gggcgcgtgg cacctcttcc
     1141 cgaagacccg gaccgtgatc gacatcggcg gccaggacac gaaggccatc aaggtgagcg
     1201 accgcggcga ggtgctcgac ttcgccatga acgacaagtg cgcggccggc agcggccgct
     1261 tcctcacgaa ctcggccgag gcgctcggga tggacgtggc gcagatcggc gcgaagtcgc
     1321 tggaggcgaa gaaccccgtg cgcctctcga cggtgtgcac gatcttcgtg gagacggaca
     1381 tcctctccta cctcgcctcg ggcaagaaga ccgaggacat cctggcgggc gtccacggcg
     1441 ccatcggctc gcgcaccgtc gcgctggtgc ggcgcgtcgg cgccgagccg gagatcacct
     1501 tcaccggcgg cgtcgcccgg aacatcggca tgatccgatc ggtccaggag aagctcgacc
     1561 tgaacatcaa cgtcagcccc gactcgcact acaccggagc tctcggtggc gcgatcttcg
     1621 cgctcgagcg catgaccgcc ggatcgcagg tgctggcatg aacgtcgacg gacgcctcgt
     1681 cgccggcatc gacgtgggcg cgcgcagcgc caaggccgtc atcctcgacg aagagcaccg
     1741 cgtcctcgga agcgggctcg cccgcatgcg tcccgacttc accggcctcg cgagggacgt
     1801 gctcacgagc gcgatcgacc aggccggcgt cacaccggac gacgtggcct acgtcgcgac
     1861 gaccggcctc ggccgctaca acatcccgtt ccgcgacctg cagatcaccg acatcaccgc
     1921 ggtcgcccgc ggcgcctcgc acctcttccc gggcacgcga tgcgtcctcg acatcggcgc
     1981 gcagagcacc cgcgcgctgc acgtgttgga gagcggccgc gtgaaggaat tccgcacgaa
     2041 cgacaagtgc gccgccggcg cgggcggctt cgccgagcgc gccgcgcggt acctcgagat
     2101 caagctcgac gacctcggtc cgctctcgct caaggccgac gccccgcagg tcatctcctc
     2161 ggtatgcgcg gtcttcgcgg agtcggagat catcaa
//
LOCUS       DASTZR010000094         2928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_110303, whole genome shotgun sequence.
ACCESSION   DASTZR010000094 DASTZR010000000
VERSION     DASTZR010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110303"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="VFV20_02130"
     CDS             <1..1140
                     /locus_tag="VFV20_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEU5287174.1"
                     /translation="VNGAGTIVGAINIAVTVLLLRCPGLSISRLPLFVWMSLMTSILI
                     LIAYPSLSAGLVMLFADRRMGAHFFDPTAGGDPLIWQHVFWFFGHPEVYIVILPAFGM
                     ISEVIPVFSRKPIFGYRFVAASSVVIVFLSVSVWAHHMFAVGMSDEANMFFSAASMAI
                     AVPTGVKVLNWTATLVGGRLRFTTAMLFAVGFIATFVMGGLTGVTLAIPPIDWVVTDS
                     YYVVAHMHYVLFGGVVFGIFAGLYYWFPKMTGRLLSERLGKLHFWFVFFGFHITFLPQ
                     HWLGVEGMPRRVWTYGADTGWAALNLLSTVGAGILGIGIAFFVWNLIVSLRSGEIAGD
                     DPWDAWTLEWSTSSPPIPENFLQIPTATSKRPLWDRKHPDRADEA"
     gene            1137..1703
                     /locus_tag="VFV20_02135"
     CDS             1137..1703
                     /locus_tag="VFV20_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEU5287175.1"
                     /translation="MTTTELPRHRLQSPPLLGVALFIASESIFFLAIIVSFVALRPLG
                     LETAKAELDLPRTFIFSLFLFASSATMTIAERRRSRDGVLWLVVTAALGAIFIAGQGL
                     EYARLLDSGISPRSGMFGTTFFTLTGLHGLHVLVGLTAIAGLAIAARSAAHGVAATAW
                     ECVAWYWHFVDGVWVVVFSVVYLGTLVG"
     gene            1700..1873
                     /locus_tag="VFV20_02140"
     CDS             1700..1873
                     /locus_tag="VFV20_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287176.1"
                     /translation="MKHHVPAPTIWPAAFATGVTFAAAGMVTSPIVVAFGAVLTLISL
                     VGWVSILVREAHE"
     gene            1870..2370
                     /locus_tag="VFV20_02145"
     CDS             1870..2370
                     /locus_tag="VFV20_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEU5287177.1"
                     /translation="MIDRRRFLSRITIALSAGMAGLVGVPILGYLLAPLVRPLPDDYV
                     DVGAVDGFALGETTQVRFRDPSPLPWAGQLAETALWVRRVSQSGAETFQVFSVHCTHL
                     GCPVNWRPEARIFLCPCHGGVYYADGTVAGGPPERPLFERDWKISAGRLFVKAQPLPT
                     VQTKEQ"
     gene            2370..>2928
                     /locus_tag="VFV20_02150"
     CDS             2370..>2928
                     /locus_tag="VFV20_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEU5287178.1"
                     /translation="MRRILRWFGERLELEDSVLAVLRHPVPASLERPVGWWYVFGSAT
                     LAVFVVMVATGVGLAMTYVPAPNSAYDSLVFITRDATLGGLVRGTHYWGAAAMVVLAL
                     IHMSQVFLFGSYKFPREINWLSGSVLLLATLAMAFTGQLLRWDQDAFWAVVVAAEQAG
                     RAPFLGDLLAKLVIAGQNVGGATLTR"
BASE COUNT          342 a         1030 c         1000 g          556 t
ORIGIN      
        1 gtgaacgggg ccggcaccat cgtgggcgcg atcaacatcg ccgtcacggt gctgctgctc
       61 cgctgccccg gcctctccat ctcgcggctc ccgctcttcg tgtggatgtc gctcatgacg
      121 agcatcctca tcctcatcgc gtatccgtcg ctgtcggcgg gcctggtgat gctgttcgcg
      181 gaccgccgca tgggcgcgca cttcttcgac ccgacggccg gcggcgaccc gctcatctgg
      241 cagcacgtct tctggttctt cggccatccc gaggtgtaca tcgtcatcct gccggcgttc
      301 gggatgatct cggaggtcat cccggtgttc tcgcgcaagc cgatcttcgg ctaccgcttc
      361 gtcgcggcgt cgagcgtggt gatcgtgttc ctgtcggtgt cggtgtgggc gcaccacatg
      421 ttcgccgtgg ggatgtccga cgaggcgaac atgttcttct ccgcggcgtc gatggcgatc
      481 gcggtgccga ccggcgtgaa ggtgctgaac tggacggcca cgctggtggg cggccgcctg
      541 cgattcacca ccgccatgct gttcgcggtc ggcttcatcg ccacgttcgt catgggcggc
      601 ctcaccggcg tgacgctggc gatcccgccc atcgactggg tggtcacgga ctcctactac
      661 gtcgtggcgc acatgcatta cgtcctgttc ggcggcgtgg tcttcggcat cttcgccggc
      721 ctgtactact ggttcccgaa gatgaccggc cgcctgctgt cggagcgcct cggcaagctg
      781 cacttctggt tcgtcttctt cggcttccac atcaccttcc tgccgcagca ctggctcggc
      841 gtcgagggca tgccgcgtcg cgtctggacg tacggcgcgg acaccgggtg ggcggcgctc
      901 aacctgctct cgacggtcgg cgcgggcatc ctcggcatcg gcatcgcgtt cttcgtgtgg
      961 aacctgatcg tgagcctgcg ctccggcgag atcgcgggcg acgacccctg ggacgcgtgg
     1021 acgctcgaat ggtccacgtc gtcgccgccc atccccgaga acttcctgca gatcccgacg
     1081 gccacgagca agcggccgct ctgggaccgc aagcatcccg atcgggcgga cgaagcatga
     1141 ccaccaccga gcttccccgc caccgcctgc agagcccgcc gctcctgggc gtcgcgctgt
     1201 tcatcgcgtc ggagtcgatc ttcttcctcg cgatcatcgt gtcgttcgtc gcgctgcggc
     1261 cgctcggcct cgagaccgcc aaggcggagc tcgacctgcc gcggacgttc atcttcagcc
     1321 tcttcctctt cgcgtcgagc gcgacgatga ccatcgcgga gcgccggcgc tcgcgcgacg
     1381 gcgtgctgtg gctcgtggtg acggcggccc tgggcgcgat cttcatcgcc ggccagggac
     1441 tggaatacgc gcgcctgctg gacagcggaa tctcgccgcg gtcggggatg ttcggcacga
     1501 cgttcttcac gctcaccggc ctgcacggcc tgcacgtgct cgtcggcctc accgcgatcg
     1561 ccggcctcgc gatcgccgcg cggagcgcgg cgcacggagt ggccgcgacc gcgtgggagt
     1621 gcgtcgcctg gtactggcac ttcgtcgacg gcgtctgggt ggtcgtcttc agcgtcgtct
     1681 atctggggac gctggtcggg tgaagcacca cgtgcccgcg ccgaccatct ggcccgccgc
     1741 gttcgcgacg ggcgtgacgt tcgccgccgc gggtatggtc acgagcccga tcgtcgtggc
     1801 gttcggcgcg gtcctcacgc tcatctcgct cgtgggctgg gtgagcatcc tcgtgcggga
     1861 ggcccacgaa tgatcgatcg ccgccgcttc ctctcgcgca tcaccatcgc gctctcggcc
     1921 ggcatggccg ggctcgtcgg cgtgccgatc ctcggctatc tcctcgcgcc gctggtccgt
     1981 ccgcttcccg acgactacgt cgacgtcggc gcggtggacg gcttcgcgct gggtgagacg
     2041 acgcaggtgc gcttccgcga tccgagcccg ctgccctggg ccgggcagct cgcggagacc
     2101 gcgctctggg tccgtcgcgt ttcgcaaagc ggcgcggaga cgttccaggt cttctcggtg
     2161 cactgcacgc acctcggctg cccggtgaac tggcgcccgg aggcgcgcat cttcctctgc
     2221 ccctgtcacg gcggtgtgta ctacgccgac ggcaccgtcg cgggcggccc gccggagcgg
     2281 ccgctcttcg agcgggactg gaagatctcc gcgggcaggc tcttcgtgaa ggcgcagccg
     2341 ctgcccaccg tgcagacgaa ggagcagtga tgcgccggat cctccgctgg ttcggtgagc
     2401 ggctcgagct cgaggactcg gtgctcgcgg tgctgcggca cccggtgccg gcatcgctcg
     2461 agcggcccgt cggctggtgg tacgtgttcg ggagcgcgac gctcgctgtg ttcgtcgtga
     2521 tggtggcgac cggcgtcggc ctcgccatga cgtacgtgcc ggcaccgaac tccgcatacg
     2581 actcgctcgt gttcatcacg cgtgacgcga cgctcggcgg gctcgtgcgc ggcacccatt
     2641 actggggcgc cgccgcgatg gtcgtcctcg cgctcatcca catgagccag gtgttcctgt
     2701 tcggctcgta caagttcccg cgcgagatca actggctctc ggggtcggtg ctgctgctcg
     2761 cgacgctcgc gatggccttc accgggcagc tgctgcgctg ggatcaggac gcgttctggg
     2821 ccgtggtcgt cgccgccgag caggccggcc gcgcgccgtt cctcggcgac ctgctggcga
     2881 agctcgtgat cgcgggacag aacgtcggcg gcgcgaccct gacgcggt
//
LOCUS       DASTZR010000095         2358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1259002, whole genome shotgun sequence.
ACCESSION   DASTZR010000095 DASTZR010000000
VERSION     DASTZR010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259002"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1578)
                     /locus_tag="VFV20_02155"
     CDS             complement(<1..1578)
                     /locus_tag="VFV20_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5287179.1"
                     /translation="MARDKATPKAATAAAKPSADAWQPPATNEFEKLGAFYLGRPYDL
                     AAKRPQPGLVLYDSKDLVTHAVCVGMTGSGKTGLCISLIEEAALDGVPTIAIDPKGDL
                     TNLMLTFPQLRPEDFRPWVNEEDAAKKGLTPDAYAAQQAETWKNGLASWGEDGERIKR
                     LRDAADFAVYTPGSNAGIPVSILASFAAPAPALRDDAELFRERVMTTATSLLALVGIE
                     ADPVQSREHVLISTLLTNAWSEGKDLDIAALVGQVQKPPVGRIGVMDLESFYPEKERQ
                     KLALALNNLLAAPGFSTWLEGVPMDIATLLRTPEGKPRVAIVSIAHLSDEERMFFVSL
                     LLNQILGWMRAQSGTTSLRALLYMDEIFGFFPPVAAPPSKRPLLTLLKQARAFGLGVV
                     LATQNPVDLDYKGLANAGTWLLGRLQTERDKARVLEGLEGVAASANASFDRAEMDTML
                     AGLGNRIFLMNNVHEDGPRLLETRWAMSYLRGPLTREQIKTLMDGKRPPIAKGGAAAA
                     ASSTQAAAANDAAEKAQA"
     gene            complement(1639..2208)
                     /locus_tag="VFV20_02160"
     CDS             complement(1639..2208)
                     /locus_tag="VFV20_02160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HEU5287180.1"
                     /translation="MTRPLLVTVIGKSARDPGDPVPARALAAAEEVGRLLAERGAVVV
                     SGGLSGVMEAVSRGAKAAGGLVVGVLPGFDKRDANAYVDIAITTGMGWMRNTLTVRAA
                     DAVIMISGGIGTLNELTVAYEDKPTVVLEGTGGWSDRIREIAYEGKHLDEAHRATLHF
                     ASTPREAVDLALELARHATGSDGRKEFQS"
     gene            complement(2205..>2358)
                     /locus_tag="VFV20_02165"
     CDS             complement(2205..>2358)
                     /locus_tag="VFV20_02165"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5287181.1"
                     /translation="GFRECGLTEIIAICFPEHTASRRVMEKAGMRFAGPARYYDIDVV
                     KYVKKH"
BASE COUNT          317 a          839 c          811 g          391 t
ORIGIN      
        1 ggcctgcgcc ttctcggccg cgtcgttcgc ggcggcggcc tgcgtcgacg acgcggccgc
       61 ggccgcaccg cccttcgcga tcggcggtcg cttgccgtcc atgagcgtct tgatctgctc
      121 gcgcgtgagc ggaccgcgga ggtacgacat cgcccagcgc gtctccagga gccgcggtcc
      181 atcctcgtgc acgttgttca tgaggaagat gcggttcccg aggccggcga gcatcgtgtc
      241 catctcggcc ctgtcgaagg aggcgttcgc gctcgccgcg acgccctcga gcccttcgag
      301 cacgcgcgcc ttgtcgcgct ccgtctgcag ccgcccgagc agccaggtgc ccgcgttggc
      361 gaggcccttg tagtcgaggt cgacgggatt ctgcgtcgcc agcacgacgc cgagcccgaa
      421 ggcacgcgcc tgcttcagca gcgtgagcag cggccgcttg gacggcggcg cggccaccgg
      481 cgggaagaag ccgaagatct cgtccatgta gagcagcgcg cgcaggctgg tcgtgccgga
      541 ttgcgcgcgc atccatccga ggatctggtt cagcaggagc gagacgaaga acatgcgctc
      601 ctcgtcggag aggtgcgcga tggagacgat cgcgacgcgc ggcttgccct cgggcgttcg
      661 cagcagcgtg gcgatgtcca tgggcacgcc ctcgagccag gtcgagaacc cgggcgccgc
      721 gagcaggttg ttgagcgcga gggcgagctt ctgccgctcc ttctcgggat agaaggactc
      781 gaggtccatc acgccgatgc gccccaccgg aggcttctgc acctgaccga cgagggcggc
      841 gatgtcgaga tccttcccct ccgaccaggc gttcgtgagc agggtggaga tcagcacgtg
      901 ctcacggctc tgcaccgggt cggcctcgat cccgacgagc gcgaggaggc tcgtcgcggt
      961 cgtcatgacc cgctcgcgga acagctcggc atcgtcacgc aaggccggcg ccggcgccgc
     1021 gaacgacgcg aggatcgaga cggggatgcc ggcgttcgac cccggggtgt acacggcgaa
     1081 gtccgccgca tcgcgcagcc gcttgatgcg ctcgccgtcc tctccccatg aggcgagccc
     1141 gttcttccag gtctccgcct gctgcgccgc gtaggcgtcg ggcgtcaggc ccttcttcgc
     1201 ggcgtcctcc tcgttcaccc agggccggaa gtcctcaggt cgcagctgcg ggaaggtgag
     1261 catgaggttc gtcaggtcgc ccttgggatc gatcgcgatg gtcggcacgc cgtcgagcgc
     1321 ggcctcctcg atgagcgaga tgcagagtcc ggtctttccg cttccggtca tcccgacgca
     1381 gaccgcatgc gtgaccagat ccttcgagtc gtacagcacg agtccgggct gcggccgctt
     1441 tgcggcgagg tcgtacggcc gcccgagata gaacgcgccg agcttctcga actcgttcgt
     1501 cgcgggtggc tgccatgcgt ctgccgacgg cttcgcggcg gcggtggccg ccttcggggt
     1561 cgccttgtcg cgtgccatgt gacacctccc gctcgatcct gcgtacggta cgccgcctcg
     1621 tgccggcggc gcttctgctc agctctggaa ctccttgcgg ccgtcgctcc cggtcgcgtg
     1681 gcgggcgagc tcgagggcca ggtcgacggc ttcccggggc gttgacgcga agtgcagcgt
     1741 cgcgcggtgc gcctcgtcga ggtgcttgcc ctcgtacgcg atctcgcgga tgcgatcgct
     1801 ccagccaccc gtgccctcga gcaccaccgt gggcttgtcc tcgtacgcga cggtgagctc
     1861 gttcagcgtg ccgatgccgc cggagatcat gatcacggcg tccgccgcgc ggaccgtgag
     1921 cgtgttgcgc atccagccca tgccggtggt gatcgcgatg tcgacgtacg cgttcgcgtc
     1981 gcgcttgtcg aagcccggca acactcccac gacgaggccg cctgccgcct tcgcgccgcg
     2041 gctgaccgcc tccatcacgc cggacagtcc gccgctcacg accaccgcgc cgcgctccgc
     2101 gaggagccgt cccacctcct cggccgccgc gagcgcgcgc gccggcacgg gatcacccgg
     2161 gtcgcgcgcg ctcttgccga tcacggtcac gagcagcggt cgcgtcaatg cttcttcacg
     2221 tacttcacga cgtcgatgtc gtagtagcgc gccggaccgg cgaaccgcat gccggccttc
     2281 tccatcaccc ggcgcgacgc cgtgtgctcc gggaagcaga tggcgatgat ctccgtcagg
     2341 ccgcattcgc ggaagccg
//
LOCUS       DASTZR010000096         2096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_162629, whole genome shotgun sequence.
ACCESSION   DASTZR010000096 DASTZR010000000
VERSION     DASTZR010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162629"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1532)
                     /locus_tag="VFV20_02170"
     CDS             complement(72..1532)
                     /locus_tag="VFV20_02170"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEU5287182.1"
                     /translation="MASRARQLFPQWLTRDQIVGLIVVMTGMAVAALDATVVGTAMPT
                     AIGDLGGIDRYSWVFASYLLVSTATTPVFGRFADVHGRKKVFFTALVLFVSASMLCGQ
                     AQSMDMLIAGRALQGLGAGGLISTGITMLGDLFDVRQRGRVQGFTASVWAVSAIVGPT
                     IGGIITQALSWRWTFYVNLPIGLIAIALLLTLHEREEHRGGGIDWTGAAVFASAAALL
                     LLGVNGTYPYATLPAAVLLGALFVAIERRVTDPLVDLALLRNPIIGLGLALNLIMGVL
                     QFATTTFVPPFAQGVLGRSPLEAGLALGAMSIAWPIGSTTTGWFLLRIGYRRAVIAGA
                     LGPVVGGAILLGLTPESSLWVLVIGALFIGLGMGVTATPLLVGVQTAVAYRQRGIVTS
                     LANFSRSLGGAVGVAALGAMLNTAIGSRAAELEALLDPHAQSSLGASAADARALLSGG
                     IHSVFVALLVIAAAGLLLAVQLPTHDLEAEVPAAQD"
     gene            1576..>2096
                     /locus_tag="VFV20_02175"
     CDS             1576..>2096
                     /locus_tag="VFV20_02175"
                     /inference="COORDINATES: protein motif:HMM:NF014601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HEU5287183.1"
                     /translation="MTDQGVPKTITVIGAGAIGGTMGAFLTDAGHDVTLVDREVAHVD
                     AINVRGLRITGVRGDRTFRVRARVPEDVRAPLGTVLLAVKAQATGDALRQVAPLLAPG
                     GVVVSMQNGLCEEEIAAVVGSARTIGCLVYYAADYLAPGHIELGSDHEIYFGELDGSI
                     TPRLLTLTAVMNDA"
BASE COUNT          346 a          722 c          757 g          271 t
ORIGIN      
        1 gaggcggaag gcatcgagac gtccattgtc cgccgccgcg cgccgcctcg atgtgacctt
       61 ggtcacgcgc gtcagtcctg ggccgccggt acctccgcct ccagatcgtg cgtgggcagc
      121 tggacggcca gcagcagtcc cgccgccgcg atgacgagca gcgccacgaa taccgaatgg
      181 ataccgccgc tcagcagcgc gcgcgcgtcg gcggcggacg cgccgaggga agactgcgcg
      241 tgcggatcga ggagcgcctc gagctcggcc gcgcgtgatc cgatcgccgt gttcagcatc
      301 gcgcccagcg ccgcgacgcc caccgcgccg ccgagtgacc gcgagaagtt cgcgaggctc
      361 gtcacgatgc cgcgctgccg gtacgcgacc gcggtctgca cgccgacgag gagcggcgtc
      421 gcggtcacgc ccatgccgag cccgatgaag agcgcgccga tgacgagcac ccagagcgac
      481 gattcgggcg tgagcccgag caggatcgcg ccgccgacca ccggaccgag cgccccggcg
      541 atgaccgcgc gccggtagcc gatgcgcagc aggaaccagc cggtcgtcgt cgagccgatg
      601 ggccacgcga tgctcatcgc gccgagcgcg aggccggcct cgagcggcga gcggcccagg
      661 acgccctgcg cgaacggcgg gacgaacgtg gtggtcgcga actgcagcac gcccatgatc
      721 aggttgagcg cgaggccgag gccgatgatc gggttgcgca gcagcgcgag gtcgacgagc
      781 ggatccgtca cgcgccgctc gatcgcgacg aagagcgcgc cgagcaggac cgccgccggc
      841 agcgtcgcgt acggataggt gccgttcacg ccgagcagca gcagcgcggc cgcgctcgcg
      901 aacaccgcgg cgccggtcca gtcgatgccg ccgccgcggt gctcctcgcg ctcgtgcagc
      961 gtgagcagca gcgcgatggc gatgaggccg atcggcaggt tcacgtagaa ggtccagcgc
     1021 cacgagagcg cctgcgtgat gatgccgccg atcgtcgggc cgacgatcgc gctcaccgcc
     1081 cacaccgacg cggtgaagcc ctgcacgcgg ccgcgctggc ggacgtcgaa gaggtcgccg
     1141 agcatcgtga tgccggtcga gatgagcccg cccgctccga gtccctgcag cgcgcggccg
     1201 gcgatgagca tgtccatcga ctgcgcctgc ccgcagagca tcgacgcgct tacgaagagg
     1261 acgagcgcgg tgaagaagac cttcttgcgt ccgtgcacgt ccgcgaagcg gccgaacacc
     1321 ggggtcgtcg cggtggacac gaggaggtac gaggcgaaga cccaggagta gcggtcgatg
     1381 ccgccgaggt cgccgatggc cgtcggcatc gccgtgccga cgacggtcgc gtcgagcgcc
     1441 gcgacggcca tgccggtcat gacgacgatg agtccgacga tctggtcgcg cgtcagccac
     1501 tgcgggaaga gttggcgagc gcgggacgcc atcacgcgcc tatggtgccg cagcgcgcga
     1561 gatacagtct gcgtcgtgac ggaccaaggc gttccaaaga ccatcacggt gatcggcgcg
     1621 ggtgcgatcg gcggcacgat gggtgccttt ctgaccgacg cgggtcacga tgtgacgctc
     1681 gtcgatagag aggtcgctca cgtcgacgcg ataaacgttc gcggtcttcg catcaccggc
     1741 gtacgcggcg accgtacctt tcgcgtgcgg gcccgcgtcc ccgaggatgt gcgcgcgccg
     1801 ctcggtactg tcctcctcgc agtgaaggcg caggcgaccg gagacgcgct gcggcaggtc
     1861 gctccactgc tcgcgccggg tggcgtcgtc gtctcgatgc agaacggcct gtgcgaggaa
     1921 gagatcgccg ccgtcgtggg cagcgcccgg acgatcggtt gtctcgtcta ctacgccgcg
     1981 gactatctcg cccccggaca catcgagctc ggaagcgatc acgagatcta cttcggcgag
     2041 ctggacggca gcatcacgcc gcggctcctg acgctcaccg ctgtgatgaa cgacgc
//
LOCUS       DASTZR010000097         8046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_528178, whole genome shotgun sequence.
ACCESSION   DASTZR010000097 DASTZR010000000
VERSION     DASTZR010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8046
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528178"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="VFV20_02180"
     CDS             <1..933
                     /locus_tag="VFV20_02180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEU5287184.1"
                     /translation="ADIAGHATPKRALEIAAAGGHSLLLVGPPGTGKTLLARALPGLL
                     PPLDPDEAVVASAVHSVAGLVDPARPLLAARPFRAPHHTASHLALVGGGSPPRPGEVS
                     LAHTGALFLDELPEFPRQVLDTLREPLEEGAITLSRAAAVATYPARVVLVAAMNPCPC
                     GHAGDPQSACTCLPDAVERYHARLSGPLMDRIDLRVHVPRLAYEHLRERGGETTRAVR
                     ERVLAARDRMRARLAGTGRRTNAELTVAEVREHCRLDEAADALAREAMRSSRLSARGV
                     HRVLRVARTIADLAATDRIRGEDLALALLLRGAA"
     gene            930..1751
                     /locus_tag="VFV20_02185"
     CDS             930..1751
                     /locus_tag="VFV20_02185"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEU5287185.1"
                     /translation="MIALSPQDSRYPPGLRDLDRPPDPLWIDGDVAALTASAIAIVGT
                     RRMTGYGERVARELAIACAAAGTVVVSGLAQGIDSTAHRGALDGGGRTIAVLGESIPS
                     FVATVRGRRRPLVPRIRERGLLASQFAAPFSARGWMFAERDATIAALASAVVVVEAGE
                     VSGALITAEAARRLGRVLYAVPGPIGSAASIGTNRLIAAGLARALTGADVLVPGRGAA
                     PPVDRVLEALRDGPLSLDDIARRARLPALLVSTRVASLLVRGQVVTLPDGRLRCA"
     gene            complement(1748..2587)
                     /locus_tag="VFV20_02190"
     CDS             complement(1748..2587)
                     /locus_tag="VFV20_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287186.1"
                     /translation="MLTGATIIDRRAIRYVTDLGPAAWIRGHLHPFAQDAGSVVPAIY
                     DDYARVFHPAARDDDPVTWRAIAEANGRTVHPEMQFGNIAGAWRDSPRPDVWTRPPTT
                     GTLSAEIASALADVLRAHTTTPERCWFAVWEGWGGFDPGTPRFDLPGRRYFLATGPID
                     AAASSVLGDWTGQSPSMWWPDDRTWFVSTEIDFTYTYVGGTRQCIDAILDDPRIEALR
                     ARSGDRITWDGDRLNASPGPPYEARAHMHLVGPLAAVLVEAAVGFVAWRVVRSLMRTR
                     SRR"
     gene            2653..4431
                     /locus_tag="VFV20_02195"
     CDS             2653..4431
                     /locus_tag="VFV20_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU5287187.1"
                     /translation="MPAPLSRILDARGATRVRISPDQRWLCYVTDITGTPQLWRIDIT
                     GGMPRRLTFDCDRVGAYRISPDGARIAYGADAGGNEKWQLWVIDADGAGARRLTERDD
                     RIHHLRAWTADGRALLVHANTRDPRYFDLWAHPVDGGAPRMLHRHDGTASGATPLADG
                     SVVVALNRRRGDEHELVLVTPDGASRRLTPDEPAARHEVVAAVPGGVIVLSDRDRDFV
                     GLATVALDGAFAWIVTADHDVEEARAAGHLDAYAVNIEGLSELHLREGDHDELMSALP
                     AGCLATDMIGDSLALEGETVAVAWARYGAPSTIFAGRRGEPMREVVPPVMAGLADDAL
                     PEARLVRWPTFDGREIPGFLLTPRGASSAPRPTVIEVHGGPEGQARPLWNPRTVALVA
                     NGFNVLQPNVRGSTGYGKAYQALDDVRLRMDSVRDLDAAAAWLADAGIAPAGKIGVMG
                     QSYGGFMTLAAVAFFPERGWAAAVDVYGIANFLTFFEHTDAWRRPLRAVEYGDPEQDR
                     DFLVSISPITKLDAIRAPIMFIHGANDPRVPIGETEQIVAALRAKGLEIDYLRYEDEG
                     HSLAKAKNRADAYPKVLAFFQRHMAP"
     gene            4450..5076
                     /locus_tag="VFV20_02200"
     CDS             4450..5076
                     /locus_tag="VFV20_02200"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEU5287188.1"
                     /translation="MREEAVRVADRLEGTLRIPDVEPRAIVVVAHPLPTHGGTMRNPL
                     VASLARAIASRGLYALRFNFRGVGASGGVWTGGAEEWRDVADAVAHARDTFHDLPVGV
                     AAYSFGALMTLRWLAEGGRADGIALVGVPLRSVSLDPQDIPPVPDGTFIVAAERDQFG
                     TAEELRRALPRARIAEVKDVDHFFVGRRDEVGRLVADELVRTLASRIQ"
     gene            5073..6119
                     /gene="trpS"
                     /locus_tag="VFV20_02205"
     CDS             5073..6119
                     /gene="trpS"
                     /locus_tag="VFV20_02205"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU5287189.1"
                     /translation="MTMETTAPRAAPSGKPVRKRILTGDRPTGRLHLGHYVGTLENRV
                     KLQDEYDTFLLVADYHMLTTRLEHLDEIGQNVRDVVLDNLAVGIDPEKVTIYLQSLVP
                     QTTELHLYFSMLVTVSRAQRIPTLKEQMRDHEITQPSYGLLGYPILQAADILCVKGNL
                     VPVGRDQESHIELTREIARRFNELFAPVFPVPDALIPSDKLLPGTDGNSKMGKSLGNA
                     IDLADDSATVKRKVMSMYTDPTRIRATDPGRVEGNPVFVYHDMFNPDVAEVADLKERY
                     RTGKVGDVEVKQKLYAAVERFLEPIRERRARYAADPKLVEEIIASGSERARAEASQTL
                     HDVRHAMQLDYFGL"
     gene            6123..7193
                     /locus_tag="VFV20_02210"
     CDS             6123..7193
                     /locus_tag="VFV20_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HEU5287190.1"
                     /translation="MPRLYALILSGGAGTRLWPRSRRSRPKQFLDLAGERTLLQETVD
                     RVAEIIPMERIFAVAPPEHRLLIHEQLPELRADHVVVEPYPRGNAAAIGLAMAALYAF
                     DPEAVVAVLPSDHVVEKRSKFRDVLIAATAAAERGDLVTLGVAPERPDTGFGYIEAGE
                     RLEVGSAVPVHRVKRFIEKPKKDVAEKMLAAGGHFWNAGMFVWRVTDIIASYREHLPN
                     TARAMDALAQAVGSPRYESVLAEVWEETDRTTIDYGVFEKATNVAVVAADIGWHDVGS
                     WARLADIVQTSENWSSDGHVAVGSTGNYAWAPGKTVALVGVDDLIVVDTPDALLIASK
                     DRSEEVKEVVDRLRREEREDLL"
     gene            7345..>8046
                     /locus_tag="VFV20_02215"
     CDS             7345..>8046
                     /locus_tag="VFV20_02215"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013439705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HEU5287191.1"
                     /translation="MAEAKQDLVVVESPTKARTLERMLGSEYKVEASFGHIRDLPKSK
                     MGIDLKTFLPEYVVPDDSEKQVRTLRREAKAAAHVWLATDLDREGEAIAWHVADVIKV
                     PASKLRRVTFHEITPQAIKDAFKHPREIDQDLVNAQQARRVVDRLVGYTMSPLLWKKI
                     RYGLSAGRVQSVALRLIVDREREIQAFKPVEYWSIEAALANHAGESFKAEVIQHNGKK
                     LEIPDGEHAAKHKAAL"
BASE COUNT         1152 a         2889 c         2893 g         1112 t
ORIGIN      
        1 gccgacatcg cgggacacgc gacgccgaag cgtgcgctcg agatcgccgc cgcaggcggg
       61 cacagcctgc tcctcgtcgg tccacccggg accggcaaga cgcttctcgc gcgggccctc
      121 cccggactgc tcccgccgct cgatcccgac gaagcggtgg tcgcgagcgc ggtccacagc
      181 gtggccggtc tggtggatcc cgctcggccg ctgctggcgg cacgtccgtt ccgcgccccg
      241 caccacaccg cgtcgcacct cgccctcgtc ggcggcgggt cgccgccgcg gccgggcgag
      301 gtgagcctcg cgcacacggg cgcgctcttc ctcgacgagc tgcccgagtt cccgcgccag
      361 gtgctcgaca cgctgcgcga gccgctcgag gagggcgcga tcacgctctc gcgcgcggcg
      421 gccgtcgcga cgtatccggc gcgcgtcgtc ctggtcgcgg cgatgaaccc gtgcccctgc
      481 ggccacgccg gcgaccccca gagcgcgtgc acctgcctgc ccgacgcggt cgagcgctat
      541 cacgcgcggc tctccgggcc gctcatggac cgtatcgacc tgcgggtgca cgtgccgcgc
      601 ctcgcgtacg agcacctgcg tgagcgcggc ggcgagacga cacgcgccgt gcgtgagcgc
      661 gtgctcgctg cgcgggaccg catgcgcgcg cgcctcgcag ggacgggacg gcgcacgaac
      721 gcggagctca ccgtcgcgga ggtccgcgag cactgccggc tcgacgaggc cgcggacgcg
      781 ctcgcgcgcg aagcgatgcg ctcgagccgc ctgtctgcgc gcggcgtcca ccgcgtcctc
      841 cgcgtcgcgc ggacgatcgc ggacctcgcc gcgaccgacc gcatccgcgg cgaggacctc
      901 gcgctcgcgc tgctgctccg cggtgccgcg tgatcgccct gtcgccgcag gactcccgat
      961 atccgcctgg tctgcgcgac ctcgaccgtc cacccgatcc gttgtggatc gacggcgacg
     1021 tcgcggcgct cacggcgtcg gcgatcgcga tcgtgggcac gcgccgcatg accggctacg
     1081 gcgaacgggt ggcgcgcgag ctcgcgatcg cctgcgcggc cgcggggacg gtcgtcgtgt
     1141 ccggcttggc gcagggcatc gacagcacgg cgcaccgcgg cgcgctcgat ggcggcgggc
     1201 ggaccatcgc cgtcctcggt gagtcgatcc cgtcgttcgt cgcgaccgtg cgcgggcggc
     1261 gccggccgct cgtgccccgg attcgcgagc gcgggctgct cgcgtcgcag ttcgcggcgc
     1321 cattctcggc acggggctgg atgttcgcgg aacgcgacgc gacgatcgcc gcgctcgcgt
     1381 cggccgtggt cgtggtggag gcgggcgagg tgtcgggcgc gctcatcacg gccgaggcgg
     1441 cgcggcgcct cggccgcgtc ctctacgcgg tgcccggccc gatcggctcg gccgcctcga
     1501 tcggcacgaa ccgcctcatc gcggcgggcc tcgctcgcgc gctcaccggt gcggacgtgc
     1561 tcgtgcccgg gcgcggcgcg gcgcccccgg tcgaccgcgt cctcgaggcg ctgcgcgatg
     1621 ggccgctgtc gctcgacgac atcgcgcggc gggcgcgcct gccggcgcta ctcgtctcca
     1681 cgcgcgtcgc gtcgctgctc gtgcggggcc aggtcgtgac gctcccggac gggaggctgc
     1741 ggtgcgccta acgccgcgat cgcgtccgca tcagggaccg gacgacgcgc caagccacga
     1801 acccgacagc ggcctcgacc agcacggcgg cgagggggcc gacgagatgc atgtgagcgc
     1861 gagcctcgta cggcgggccg ggcgacgcgt tgagccggtc gccgtcccag gtgatgcgat
     1921 cgccgctccg cgcgcggagc gcttcgatcc gcgggtcgtc gaggatcgcg tcgatgcact
     1981 ggcgcgttcc gccgacgtag gtgtacgtga agtcgatctc ggtggacacg aaccatgtgc
     2041 ggtcatcggg ccaccacatg ctcggcgact gccccgtcca gtccccgagg accgacgacg
     2101 ccgcggcgtc gatgggaccg gtcgcgagga agtaccggcg gcccggcagg tcgaagcgcg
     2161 gcgtcccggg atcgaagccg ccccagccct cccacacggc gaaccagcac cgctccggag
     2221 tggtggtgtg cgcacgcagg acgtcggcga gcgcggatgc gatctccgcc gacagcgtgc
     2281 cggtcgtggg cggcctggtc cagacgtcgg gccgcgggct gtcgcgccag gcacccgcga
     2341 tgttgccgaa ctgcatctcg ggatggaccg tccggccgtt cgcctcggcg atcgcccgcc
     2401 acgtcaccgg atcgtcgtcg cgtgccgccg ggtggaagac gcgcgcgtag tcgtcgtaga
     2461 tcgccggcac gaccgagccc gcgtcctgcg cgaacggatg tagatgcccg cggatccacg
     2521 ccgcgggccc gaggtcggtg acgtagcgga tcgctcgacg gtcgatgatg gtcgctcctg
     2581 tcagcatgat gcgccccctc cgcggcacgc cgggcgcctg cgctacccgt agcgggctgg
     2641 cacactcggc gggtgcccgc accgctcagc cgcatcctgg acgcgcgcgg cgcgacgcgc
     2701 gtgcgcatct cacccgacca gcggtggctc tgctacgtca ccgacatcac cgggaccccg
     2761 cagctctggc gcatcgacat cacgggcggc atgccacgcc gcctcacgtt cgactgcgac
     2821 cgcgtgggtg cgtaccgcat ctcgccggac ggcgcgcgga tcgcgtacgg cgccgatgct
     2881 ggcggtaacg agaagtggca gctctgggtg atcgacgcgg acggcgcggg cgcgcggcgg
     2941 ctgaccgaac gcgacgatcg catccatcac ctgcgcgcct ggaccgcgga tgggcgcgcg
     3001 ctgctcgtgc acgcgaacac ccgggacccg cgctacttcg acctgtgggc gcaccccgtc
     3061 gacggcggcg cgccgcgcat gctgcaccgg cacgacggca ccgcgtccgg cgcgacccct
     3121 ctcgcggacg gatcggtcgt cgtcgcgctg aaccggcggc gcggcgacga gcacgagctg
     3181 gtgctcgtga ccccggacgg cgcctcacgg cggctcacgc cggacgagcc ggccgcgcgg
     3241 cacgaggtcg tggccgcggt gcccggcggc gtcatcgtcc tgagcgatcg ggaccgcgac
     3301 ttcgtgggcc tcgcgaccgt cgccctcgac ggggcattcg cgtggatcgt cacggcggac
     3361 cacgacgtgg aggaggcgcg cgcggccggg cacctcgacg cctatgccgt gaacatcgag
     3421 gggctctccg agctgcacct gcgagagggc gaccacgacg agctcatgtc cgcgctgccc
     3481 gcggggtgtc tcgcgaccga catgatcggc gacagcctcg cgctcgaagg cgagacggtc
     3541 gccgtcgcgt gggcgcgcta cggcgcccca tccacgatct tcgctggacg gcgcggtgag
     3601 ccgatgcgcg aggtcgtccc gccggtcatg gccggcctcg cggacgacgc gctgcccgag
     3661 gcgcgcctgg tgcgctggcc gacgttcgac ggccgcgaga tcccgggctt cctgctcaca
     3721 ccgcgcggcg cgtcgagcgc gccgcggccc accgtcatcg aggtgcacgg cgggccggag
     3781 gggcaggcgc gtccgctctg gaacccgcgc accgtcgcgc tcgtggcgaa cggcttcaac
     3841 gtgctgcagc cgaacgtgcg cggatcgacc ggatacggca aggcgtacca ggcgctcgac
     3901 gatgtgcgcc tgcgcatgga ctcggtgcgc gatctcgacg cggcggcggc gtggctcgcg
     3961 gacgcgggca tcgcgccggc cggcaagatc ggcgtgatgg gccagtccta cggcggcttc
     4021 atgacgctcg ccgcggtcgc gttcttcccg gagcgcggct gggccgccgc ggtcgacgtg
     4081 tacggcatcg cgaacttcct cacgttcttc gagcacaccg acgcgtggcg ccggccgctg
     4141 cgcgcggtgg agtacggcga cccggagcag gaccgcgatt tcctggtctc gatctcgccg
     4201 atcacgaagc tcgacgccat ccgggcgccg atcatgttca tccacggcgc gaacgatccg
     4261 cgcgtgccga tcggagagac ggagcagatc gtcgccgcgc tgcgtgcgaa agggctcgag
     4321 atcgactacc tgcgctacga ggacgagggg cactcgctcg cgaaggcgaa gaaccgggcc
     4381 gacgcgtatc cgaaggtact cgccttcttc cagcgccaca tggcaccgta gcggccggac
     4441 gctggttcga tgcgtgagga agcggtgcgc gtcgccgatc gcctcgaggg cacgctgcgg
     4501 atccccgacg tcgagccgcg cgccatcgtc gtcgtggcgc atccgctgcc cacgcatggg
     4561 gggacgatgc gcaacccgct cgtggcctcg ctcgcgcgcg cgatcgcgtc gcgcggcctc
     4621 tacgcgctgc ggttcaactt ccgcggggtg ggcgcgagcg gcggcgtgtg gaccggtggc
     4681 gccgaggagt ggcgcgacgt ggccgacgcg gtcgcgcacg cgcgcgatac cttccacgat
     4741 ctgccggtcg gcgtcgcggc gtactcgttc ggcgcgctca tgacgctgcg ctggctcgcc
     4801 gagggcgggc gcgcggacgg gatcgcgctc gtcggcgtcc cgctgcgctc cgtgtcgctt
     4861 gacccgcagg acatcccgcc ggtcccggac gggacgttca tcgtcgcggc ggagcgcgat
     4921 cagttcggca ccgcggagga gctgcgccgc gcgctcccgc gcgcgcgcat cgcggaggtg
     4981 aaggacgtcg accacttctt cgtcgggagg cgcgacgagg tgggacgtct ggtcgccgac
     5041 gagctggtgc gcacgctagc atcgagaatc caatgaccat ggaaacgacg gcgccgcgcg
     5101 ccgcgccatc gggcaagccc gtgcgcaagc ggatcctcac cggggatcgg cccacgggcc
     5161 gcctccacct gggccactac gtcggcacgc tcgagaaccg cgtgaagctg caggacgagt
     5221 acgacacgtt cctgctcgtc gcggactacc acatgctcac gacgcggctc gagcacctcg
     5281 acgagatcgg gcagaacgtc cgcgacgtcg tgctcgacaa cctggccgtc ggcatcgacc
     5341 cggagaaggt cacgatctac ctccagtcgc tggtgccgca gacgacggag ctgcacctgt
     5401 acttctcgat gctcgtcacg gtctcgcgcg cgcagcgcat ccccacgctg aaggagcaga
     5461 tgcgcgacca cgagatcacg cagccctcct acgggctcct cggctacccg atcctccagg
     5521 ccgcggacat cctgtgcgtg aaggggaacc tggtgccggt cggccgtgac caggagtcgc
     5581 acatcgagct cacgcgcgag atcgcgcgcc gcttcaacga gctgttcgcg ccggtcttcc
     5641 cggtgcccga cgcgctcatc ccgagcgaca agctcctgcc cgggacggac gggaactcca
     5701 agatggggaa gagcctcggc aacgccatcg acctcgcgga cgacagcgcg acggtgaagc
     5761 gcaaggtcat gtcgatgtac accgatccga cgcgcatccg tgcgacggac ccggggcgcg
     5821 tcgagggcaa tccggtcttc gtctaccacg acatgttcaa ccccgacgtg gccgaggtcg
     5881 cggacctgaa ggagcgctac cgcacgggca aggtgggcga cgtcgaggtg aagcagaagc
     5941 tctacgccgc ggtcgagcgc ttcctggagc cgatccgcga acgccgcgcc cggtacgccg
     6001 cggatccgaa gctcgtcgag gagatcatcg cgagcgggag cgagcgcgcc cgtgccgagg
     6061 cgtcgcagac gcttcacgac gtccgccacg cgatgcagct cgactacttc ggcctctagg
     6121 cgttgcctcg gctctacgca ctcatcctct ccggcggcgc gggcaccagg ctctggccac
     6181 gctcacggcg ttcccggccg aagcagttcc tcgacctcgc cggcgagcgc acgctgctgc
     6241 aggagacggt cgatcgcgtg gccgagatca tcccgatgga gcgcatcttc gccgtcgcgc
     6301 cgcccgagca tcgcctgctg atccacgagc agctccccga gctgcgggcc gatcacgtcg
     6361 tcgtcgagcc gtatccccgc ggcaacgccg ccgcgatcgg cctcgcgatg gccgcgctct
     6421 acgcgttcga cccggaggcg gtcgtggccg tgctgccgtc cgaccacgtc gtcgagaagc
     6481 gctcgaagtt ccgtgacgtg ctcatcgccg ccacggccgc ggccgagcgc ggggatctgg
     6541 tgacgctcgg cgtcgccccc gagcggcccg acacgggttt cggctacatc gaggccgggg
     6601 agaggctcga ggtcggatcc gcggtgccgg tgcatcgcgt gaagcgcttc atcgaaaagc
     6661 cgaagaagga tgtcgccgaa aagatgctgg cggcgggcgg ccacttctgg aacgccggga
     6721 tgttcgtctg gcgcgtgacc gacatcatcg cctcgtaccg cgagcatctc ccgaacaccg
     6781 ctcgcgcgat ggacgcgctg gcgcaggcgg tcggctcgcc gcggtacgag tccgtcctcg
     6841 ccgaggtgtg ggaggagacc gaccggacga cgatcgacta cggcgtattc gagaaggcga
     6901 cgaacgtggc ggtcgtggcg gcggacatcg gctggcacga cgtgggcagc tgggcgcgcc
     6961 tcgcggacat cgtccagacg tcggagaact ggtcgtcgga cgggcatgtc gcggtcggct
     7021 ccaccgggaa ctacgcctgg gcgcctggta agaccgtcgc cctggtgggt gtcgacgacc
     7081 tgatcgtggt ggacacgccg gatgccctgc tcattgcgtc gaaggaccgc tccgaggagg
     7141 tcaaggaggt ggtcgaccgg ctccgccggg aggagcggga ggacctgctt taggccgaaa
     7201 tccgcgaaaa tccgccgaaa ccgcgtcggg acgggtgccc ggaccgctct atatctaaat
     7261 agagcgtcta ggcggtgccg ctagagttat aggcagtggg gggcccgtga gggccctttt
     7321 tgttgcgcga aaggaagttg agccttggcg gaagcaaagc aggacctcgt cgtcgtcgaa
     7381 tcgccgacga aggccaggac gctcgaacgg atgctcggta gcgagtacaa ggtcgaagcg
     7441 tccttcgggc acatccggga tctgcccaag tcgaagatgg gcatcgatct caagacgttc
     7501 ctgcccgaat atgtggtccc ggacgattcc gagaagcagg tccgcaccct ccgccgggag
     7561 gccaaggcgg cagcgcatgt ctggctggcg accgacctcg accgcgaggg cgaggcgatc
     7621 gcctggcacg tggccgacgt catcaaggtc ccggcgagca agctccgccg cgtcaccttc
     7681 cacgagatca ccccccaggc catcaaggac gccttcaagc acccgcgcga gatcgaccag
     7741 gacctggtca acgcgcagca ggcgcggcgc gtcgtcgatc gcctcgtcgg ctacacgatg
     7801 tcgccgctgc tctggaagaa gatccgctac ggcctctcgg ccggccgcgt ccagtcggtc
     7861 gcgctccggc tcatcgtcga tcgcgagcgc gagatccagg cgttcaagcc cgtcgagtac
     7921 tggagcatcg aggccgcgct cgcgaaccac gccggcgagt ccttcaaggc cgaggtcatc
     7981 cagcacaacg gcaagaagct cgagatcccg gatggcgaac acgccgccaa gcacaaggcg
     8041 gcgctc
//
LOCUS       DASTZR010000098         3614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_813972, whole genome shotgun sequence.
ACCESSION   DASTZR010000098 DASTZR010000000
VERSION     DASTZR010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813972"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VFV20_02220"
     CDS             complement(<1..456)
                     /locus_tag="VFV20_02220"
                     /inference="COORDINATES: protein motif:HMM:NF014166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEU5287192.1"
                     /translation="MRRRSTRTSTSPTRCSSTRSLTVLGIDPGTTGMGYALLELDRDP
                     PRLVRCGLVPTPRDGSAAERLLGIANALDALIAEHAPGALALERLYFNKNVKTAMSVA
                     EARGIALVCGARAGLQIAEYTPQEVKQSVTGNGGSEKKAVQLMIRHILKL"
     gene            complement(398..1141)
                     /locus_tag="VFV20_02225"
     CDS             complement(398..1141)
                     /locus_tag="VFV20_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU5287193.1"
                     /translation="MSGHSKWSQIKRQKGAADVKRGVVFTKMTREIMLAAREGGPNPE
                     ANFRLRLAVDRARAVNMPHANIQRAIERATGGGEGAQIESVVYEGYGPGGVSVMVEAA
                     TDNRNRTASEVRAAFVRHGGKLGESGSVQWLFEQKGVIEIDTAGRSADEVQLVAIDAG
                     AEDVEVDGALITVYTTPASFEKVKKAIEVAGIPIGSAEISMRPTTTVRVEGDKAQQVM
                     RLVEALEDLDDAQKVHANFDIPDEVLQHA"
     gene            1199..3283
                     /gene="fusA"
                     /locus_tag="VFV20_02230"
     CDS             1199..3283
                     /gene="fusA"
                     /locus_tag="VFV20_02230"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU5287194.1"
                     /translation="MPSVAPDKIRNVAVVGHGGSGKTTLVESILFAAGATTRLGKVDD
                     GTSILDTDPEEHKRKITINVAIASFQFDGTKINFLDTPGFADFAGDQRAALRVADAAI
                     VVVDASAGVQVGTQLVWSDLEKAKTPRAVVVSRLDRENADFDRVLGQLRQAYGIRVVP
                     LHVPVGDHQAMSGTIDLLHSTLLKGPKDKPVEMPAADKERVAAYRQQLVEAIVETDED
                     LLTRYLDGKELAYDELRDALHTAVREGKVVPVIATSSSRNVGYGALLTTISEMLPSPA
                     EVGSIIARSPSGDEVARKVDPGEKLSAFVFKTLADPFVGKLSYVRVYSGTLHHNSQVF
                     DTQKNEAERVGQIFFIRGKEQEATDVVGAGDICAIPKLTATSTNATLTDKDAPVTLPA
                     IDFPPPSFSVAIEPQSKADLDKLGTALHKLIDEDPSLHVRRDDATHETILSGIGESAI
                     DTAVHHLKEKFGVAVQTRTPRVPYKESIRRKASAQGRYKKQTGGHGMFGDTWIEVEPL
                     PAGSGVEFATRIVGGSVPRNFWPAVEKGIREAAERGVIAGYPLSDFRATLYDGSFHAV
                     DSNEMSFRVAGSLGLQACVREADAYLLEPIHELEVLVPEEQMGDVLADLNGRRGRVLG
                     MESTGAGYQSIKAHVPLAEIFRYATDLRSMTGGRGSFSSRFLSYEECPSHIAQKVIAA
                     HEEPDEETAAAH"
     gene            complement(3287..>3614)
                     /locus_tag="VFV20_02235"
     CDS             complement(3287..>3614)
                     /locus_tag="VFV20_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEU5287195.1"
                     /translation="LNFNVFADAHGVVLLDWGASLYGDFLYDAALLTFWWPWYRKWDA
                     IDIRAELEQHHMRAGTRLPRFAERLRCYEVHIGVGHIAYQVGRGRIENARWTARHTVE
                     VANAPL"
BASE COUNT          571 a         1251 c         1231 g          561 t
ORIGIN      
        1 gagcttcagg atgtggcgga tcatcagctg caccgccttc ttctcggagc cgccgttacc
       61 cgtcacggac tgcttcacct cttgcggcgt gtactcggcg atctggaggc cggcgcgtgc
      121 gccgcagacg agcgcgatcc cacgcgcctc ggcgacgctc atcgcggtct tcacgttctt
      181 gttgaagtag aggcgctcga gggcgagcgc acccggtgcg tgctcggcga tgagcgcgtc
      241 cagcgcgttc gcgatcccga gcaggcgctc ggcggcgctg ccgtcgcgcg gggtcggcac
      301 gaggccgcag cggacgaggc gcgggggatc gcggtcgagc tcgaggagcg cgtatcccat
      361 gccggtcgtc cccggatcga tgccgaggac ggtgagacta cgcgtgctgg agcacctcgt
      421 cggggatgtc gaagttcgcg tggaccttct gcgcatcgtc gagatcctcg agcgcctcga
      481 cgagccgcat cacctgctgc gccttgtcgc cctcgacgcg cacggtggtc gtcggccgca
      541 tcgagatctc ggccgagccg atcgggatgc ccgccacctc gatcgccttc ttcaccttct
      601 cgaacgacgc cggggtggtg tagacggtga tgagcgctcc gtcgacctcc acgtcctccg
      661 cgccggcgtc gatcgcgacg agctggacct cgtcggcgga ccgtcccgcg gtgtcgatct
      721 cgatcacgcc cttctgctcg aagagccact gcaccgaacc ggactcgccg agcttgccgc
      781 cgtgccgcac gaacgccgcg cggacctcgg atgcggtgcg attgcggttg tcggtggcgg
      841 cctcgaccat cacggacacg ccgcccggcc cgtagccctc atacaccacg ctctcgatct
      901 gcgcgccctc gccgccgccg gtcgcgcgct cgatcgcccg ctggatgttg gcgtggggca
      961 tgttgaccgc tcgcgcccgg tcgaccgcga ggcgcagcct gaagttggcc tcaggattcg
     1021 gcccgccctc gcgggcggcg agcatgatct cgcgggtcat cttggtgaag acgacgcccc
     1081 gcttcacgtc ggccgcgccc ttctggcgct tgatctggga ccactttgag tgaccggaca
     1141 tcggctgaat agtatgtccg agcacactgt cgtggactag cgcttagggg agaagcagat
     1201 gccttctgtc gctccagaca agatccggaa cgtcgcggtg gtcggtcatg gcggctcggg
     1261 aaagacgacg ctcgtggaga gcatcctgtt cgcggcgggc gcgaccacgc ggctcggcaa
     1321 ggtcgacgat ggcacgagca tcctcgacac cgatcccgag gagcacaagc gcaagatcac
     1381 catcaacgtc gcgatcgcgt cgttccagtt cgacgggacg aagatcaact tcctggacac
     1441 gccgggcttc gcggacttcg ccggcgacca gcgcgccgcg ctccgtgtcg cggacgccgc
     1501 gatcgtcgtc gtggacgcgt cggccggggt ccaggtgggc acgcagctgg tctggtccga
     1561 tctcgagaaa gcgaagaccc ctcgcgcggt cgtcgtcagc cgtctggacc gggagaacgc
     1621 cgacttcgac cgcgtgctcg gacagctgcg ccaggcgtac ggcatccgcg tcgtgccgct
     1681 gcacgtgccg gtcggcgatc accaggccat gtccggcacg atcgacctcc tccacagcac
     1741 cctgctgaag gggccgaagg acaagccggt ggagatgccg gccgcggaca aggaacgcgt
     1801 cgccgcctac cgccagcagc tggtggaggc catcgtcgag acggacgaag atctcctcac
     1861 gcgatacctc gacggcaagg agctcgcgta cgacgagctc cgggacgcgc tccacaccgc
     1921 ggtgcgcgag ggcaaggtcg tgccggtcat cgcgacgtcc tcgtcgcgga acgtcgggta
     1981 cggcgcgctg ctcaccacca tcagcgagat gctcccctca cccgccgagg tgggttcgat
     2041 catcgcgcgg tcgccgtccg gcgacgaggt cgcgcgcaag gtcgatccgg gcgagaagct
     2101 ctcggccttc gtcttcaaga cgctcgcgga cccgttcgtc gggaagctct cctacgtgcg
     2161 ggtgtactcg gggacgctgc accacaactc ccaggtgttc gacacgcaga agaacgaggc
     2221 cgagcgcgtc gggcagatct tcttcatccg cgggaaggag caggaggcga ccgacgtcgt
     2281 cggcgcgggc gacatctgcg cgatcccgaa gctcaccgcg acgtccacga acgcgacgct
     2341 caccgacaag gacgccccgg tgacgctgcc cgccatcgac ttcccgccac cgtcgttctc
     2401 ggtcgcgatc gagccgcagt cgaaggccga tctcgacaag ctcgggacgg cgctgcacaa
     2461 gctcatcgac gaggacccgt cgctgcacgt ccgccgcgac gacgcgacgc acgagacgat
     2521 cctctcgggt atcggcgagt cggcgatcga cacggcggtc catcacctca aggagaagtt
     2581 cggcgtcgcg gtgcagacgc gcacgccccg cgtcccgtac aaggagtcca tccggaggaa
     2641 ggcgtcagcg caggggcgct acaagaagca gaccggtggc cacggcatgt tcggcgacac
     2701 ctggatcgag gtggagccgc ttcccgcggg cagcggtgtc gagttcgcga cacgcatcgt
     2761 gggtggttcg gtgccgcgca acttctggcc ggccgtcgag aagggcatcc gcgaggccgc
     2821 cgagcgcggc gtcatcgcgg ggtatccgct ctcggacttc cgcgccacgc tgtacgacgg
     2881 ctccttccac gcggtcgact cgaacgagat gagcttccgc gtcgcgggct cgctggggct
     2941 gcaggcctgc gtccgtgagg ccgacgccta cctcctcgag ccgatccacg agctcgaggt
     3001 gctcgtgccg gaggagcaga tgggcgacgt cctcgccgat ctgaacggcc ggcgcggtcg
     3061 cgtcctcggc atggagtcga cgggcgcggg ctaccagagc atcaaggcgc acgtcccgct
     3121 cgcggagatc ttccgctacg cgaccgacct ccgctcgatg accggcgggc gcggcagctt
     3181 cagctcgcgc ttcctgagct acgaggagtg tccctcgcac atcgcgcaga aggtcatcgc
     3241 cgcgcacgag gagccggacg aggagaccgc cgccgcgcat tagtcgctag agcggcgcgt
     3301 tcgcgacctc gaccgtgtgg cgcgccgtcc agcgcgcgtt ctcgatgcgt ccgcgtccca
     3361 cctggtacgc gatgtggccg actccgatgt ggacctcgta gcagcggagg cgctcagcga
     3421 accgcggcag cctcgtaccg gcacgcatgt ggtgctgctc gagctccgcg cggatgtcga
     3481 tggcatccca cttcctgtac cagggccacc agaacgtgag gagcgcggcg tcgtacagga
     3541 agtcgccgta gagcgaggcg ccccagtcca gcagcaccac gccatgggcg tccgcgaaga
     3601 cgttgaagtt cagc
//
LOCUS       DASTZR010000099         6572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1050837, whole genome shotgun sequence.
ACCESSION   DASTZR010000099 DASTZR010000000
VERSION     DASTZR010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6572
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050837"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VFV20_02240"
     CDS             <1..499
                     /locus_tag="VFV20_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU5287196.1"
                     /translation="AVSIALGTPIRWMAKIEVFDYPIIGFLLRGVGNFSIRRGESDRR
                     AIGLAIRVLEAGLPLGIFPEGHRSETGALLRGQPGIALLAERSDALIVPCGIVGTRTA
                     MPHLPRRTEVTLHFGPAFRFSDLPADVRADRQATADAIMRRIAVLLPAEMRGAYAHGI
                     DRAST"
     gene            510..1358
                     /gene="ispH"
                     /locus_tag="VFV20_02245"
     CDS             510..1358
                     /gene="ispH"
                     /locus_tag="VFV20_02245"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEU5287197.1"
                     /translation="MEVLLARENGFCFGVKKAVELTEAAAETGKQVHNLGQVVHNPKI
                     SERLQARGVKVIKDPADAQEGIVVIRAHGVPPDVRAKIEERGLECIDATCSLVLRAQR
                     FTKQLADEGYTVILLGTPDHPEVIGLKAFAGDNYVIVESEDEWKKLPKMKRAGVVSQS
                     TQPPWAFTALVAHVATIAQEMKVYNTVCPVTVKRQVAASELAEQVRTIIVVGGKNSAN
                     TRELVSLAKMQGRDAYHIEAADEVRAEWIQGQERVGLIGGCSTPMDTLLEVKERLEAL
                     AEPVLA"
     gene            1364..2677
                     /gene="der"
                     /locus_tag="VFV20_02250"
     CDS             1364..2677
                     /gene="der"
                     /locus_tag="VFV20_02250"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEU5287198.1"
                     /translation="MARAVVAIVGRPNVGKSTLFNRLVGERIAIVEDVPGTTRDRIYA
                     TTEWRGREFSLIDTGGLEDPRAGEMEAAVRRQAEAAIAEADVVMFLVDAQSGVLPVEH
                     DVADLLRRSRKPVLLVANKADSWRGEAQSAEFYELGLGTPLTVSAIQGIGTGDLLDAV
                     VERLPPETVEVESTDAHIAIVGRPNVGKSSFLNTLVGTERAVVSEIPGTTRDSTDTAF
                     ELDGRRVVLVDTAGIRRRGRIEEGIEKYALLRTARALERSDVAILLTDATEGVTAQDV
                     HIAGYALEAAVGLVLAVNKWDVVDRGAEMTASVEAELAREFHFAPWMPRHFVSAKTGR
                     NVRETLADALRVVDERRRKIPTSELHRLLMEAVEAHPPTPHRGKEVRFTHVTQARGKA
                     PTFVFFVDPSEGVHFTYQRYLENRIRERYPFPGTPIKLEFKAAKD"
     gene            2683..3315
                     /gene="plsY"
                     /locus_tag="VFV20_02255"
     CDS             2683..3315
                     /gene="plsY"
                     /locus_tag="VFV20_02255"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEU5287199.1"
                     /translation="MPATLIAFAIVALVGYLVGSISSGYIVGRVYRNVDLRTVGSGST
                     GATNTFRTLGRGAGVLVASLDILKGALAVVFAQIVFPEGSAGRPLAEALGAVCAVAGH
                     CWPLTLRGRGGRGVATGFGALLFVATPAWASAVAAFAIAIAFTRMVSVGSLASVIGAL
                     AGYLLFSATGWLSFHWATLAFILVAGTIVYVRHLPNIQRIARGVEPRLGS"
     gene            3312..4367
                     /locus_tag="VFV20_02260"
     CDS             3312..4367
                     /locus_tag="VFV20_02260"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU5287200.1"
                     /translation="MSPVSVLQAGSWGTTLATILARDAGRDVVLWTRDAEQARAMAAE
                     RENRRYLPGIRIPERVVITADVEQALSHAEDLIVAAPSVGVRALRERIAPLLGPRHHI
                     LSATKGLDGDGRRMSELWAEVVSPGRVAVLSGPNISREIAAGHPAASVVASVEPETAK
                     RFQTIVGTPMFRAYTDADVVGVELCGALKNIVALGAGAIDGMEYGDNAKAGFMTRGLA
                     EIARYGFACGANPLTVAGLAGVGDLIATCMSKHSRNRTVGELLAKGVRLPEIRERLGG
                     QIAEGLTTAEATYRAARDKSVSMPITEQTHLVLSEGKSIREAMRDLMRRERGEEVAGP
                     LAEISRLIASAAAARRG"
     gene            4525..5889
                     /locus_tag="VFV20_02265"
     CDS             4525..5889
                     /locus_tag="VFV20_02265"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU5287201.1"
                     /translation="MALRVLVTRAETGAPVEGARVCATPSRRAESCATAGKDGTVAFR
                     GAPGTYFVRVSGPAEQRYVEAQRVADLSNGDGALWVELTPQHRISGRVRDADAKPVAG
                     AEACAHPSGDDPPVCARSGADGAYVIDAKAGVYRLEVTGAPGARLVSQWARGRAFLEE
                     ADILDARTADVPDVDVTLVRGVVLQGIVRFGGVPVEDAQVCIRTLAAPLPWECERTDK
                     KGHYAALREPGAYYVWTVPPADIRAVPQWYDRALTGVGSSVLRLDGDATLDVALTTGP
                     TLSGVLRTADGQPVRNALVCIDTAFASGRICRETASTGRYSITTRPETYIVSVIPPAH
                     SGLIGEYWNGKRTWKDADEVFVGGDVILDLTVARGATVTGTVKDKRGIAVAGATVNLW
                     DERGIADATDTDSAGRFEAVVRPGSLSLEVFPPFVANLVGKTQTVDVVGDMELQVTLD
                     DIAP"
     gene            5986..6399
                     /gene="sodN"
                     /locus_tag="VFV20_02270"
     CDS             5986..6399
                     /gene="sodN"
                     /locus_tag="VFV20_02270"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011125519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HEU5287202.1"
                     /translation="MLGRLLKPTTDAYAHCDIPCGIYDPTPAKIAADTVAKMVEKIGA
                     LDKSATDFTTRGNFVRMVTVKEQHAEICKKELQVLWSDYFKPEHLEKFPKLHDTFWKA
                     LKLASKNKQSVDAQAAADLQAAVKEISDMFWATKK"
BASE COUNT         1033 a         2224 c         2370 g          945 t
ORIGIN      
        1 ggcggtctca atcgcactcg ggacgccgat acggtggatg gcgaagatcg aggtgttcga
       61 ctaccccatc atcgggtttc tgctgcgagg cgtcgggaac ttctcgatcc gccgcggcga
      121 gagcgatcgg cgcgcgatcg ggctcgccat ccgcgtgctc gaagcggggc tgccgctcgg
      181 catcttcccc gagggacacc gcagcgagac gggtgcgctc ctgcggggcc agccgggcat
      241 cgcgcttctg gccgagcgca gcgacgcgct catcgtgccg tgcgggatcg tgggcacgcg
      301 caccgcgatg ccgcatctgc cgcgccgcac cgaggtcacg ctgcacttcg gtccggcctt
      361 ccgcttctcg gaccttcccg cggacgtccg cgcggaccgt caggccacgg cggacgcgat
      421 catgaggcgc atcgccgtgc tgctgcccgc cgagatgcgt ggcgcgtacg cgcacggtat
      481 cgaccgagcg agcacgtaga ctttgatgga tggaagtact cctcgcgcga gagaacgggt
      541 tctgcttcgg tgtgaagaag gcggtcgagc tgaccgaggc cgccgccgag accggaaagc
      601 aggtgcacaa cctcggacag gtcgtgcaca accccaagat cagcgagcgg ctccaggcgc
      661 gtggcgtgaa ggtcatcaag gatcccgccg atgcgcagga agggatcgtc gtcatccggg
      721 cgcacggcgt accgccggac gtgcgagcga agatcgagga gcgcggcctc gagtgcatcg
      781 acgcgacgtg ctcgctcgtg ctccgcgcgc agcggttcac gaagcagctg gcggatgagg
      841 gctacaccgt catcctgctc gggacacccg accaccccga ggtcatcggg ctgaaggcat
      901 tcgccggcga caactacgtc atcgtcgaga gcgaggacga gtggaagaag ctccccaaga
      961 tgaagcgcgc gggcgtcgtc tcgcagtcga cgcagccgcc gtgggcgttc acggcgctcg
     1021 tcgcgcacgt cgcgaccatc gcgcaggaga tgaaggtcta caacaccgtg tgcccggtga
     1081 ccgtgaagcg gcaggtcgcc gcgagcgagc tcgccgagca agtccgcacc atcatcgtgg
     1141 ttggtggaaa gaactcggcg aacacgcgtg agctcgtgag cctcgcgaag atgcagggac
     1201 gggacgccta tcacatcgaa gcggccgacg aggtgcgcgc cgagtggatc cagggtcagg
     1261 agcgcgtcgg cctcatcggc ggatgctcga cacccatgga tacgctgctc gaggtgaagg
     1321 agcggctcga agctctcgcg gaaccggtcc tcgcataacg acgatggcgc gggccgtcgt
     1381 cgccatcgtc gggcgtccga acgtggggaa gtccacgctg ttcaaccgcc tcgtcgggga
     1441 acgcatcgcc atcgtcgagg acgttccagg cacgacgcgt gaccgcatct acgcgacgac
     1501 cgagtggcgc ggccgcgagt tctcgctcat cgacacgggc gggctcgagg acccccgcgc
     1561 aggagagatg gaggccgcgg tgcggcggca ggccgaggcc gcgatcgccg aggccgacgt
     1621 ggtgatgttc ctcgtcgacg cgcagtccgg cgttctgccc gtcgagcacg acgtcgccga
     1681 cctcctgcgc cgcagccgaa agccggtgct gctcgttgcg aacaaggccg attcgtggcg
     1741 cggcgaggcg caatccgccg agttctacga gctggggctc gggaccccgc tcaccgtgtc
     1801 ggccatccag ggcatcggca cgggcgacct gctggacgcc gtggtcgagc gccttccacc
     1861 cgagacggtg gaggtcgagt cgacggacgc gcacatcgcg atcgtcggac ggccgaacgt
     1921 gggaaagtcc agcttcctca acacgctcgt cggcacggag cgcgcggtcg tgagcgagat
     1981 cccgggcacg acgcgcgaca gcaccgacac cgcgttcgag ctcgatggac gccgcgtcgt
     2041 gctcgtcgac accgccggga tccggcgccg cgggcggatc gaggagggca tcgagaagta
     2101 cgcgctgctg cgcacggcac gcgccctcga gcgatccgac gtcgccatcc tgctcaccga
     2161 cgcgaccgag ggcgtcaccg cgcaggatgt gcacatcgcc gggtacgcgc tcgaggcggc
     2221 ggtgggtctg gtgctggcgg tgaacaaatg ggacgtggta gaccgtggcg cggagatgac
     2281 ggccagcgtc gaagccgagc tcgcacgcga attccacttc gcgccctgga tgccgcgcca
     2341 cttcgtctcc gccaagaccg gccgcaacgt ccgcgagacg ctcgccgacg cgctgcgggt
     2401 cgtcgacgaa cgccgccgga agatccccac ctccgagctg catcgtctcc tcatggaggc
     2461 cgtcgaggcg cacccgccga cgccgcatcg cggcaaggag gtccgcttca cgcacgtgac
     2521 gcaggcgcgt ggcaaggcgc ccaccttcgt cttcttcgtc gatccgtccg agggcgtcca
     2581 cttcacgtac cagcgctacc tggagaaccg catccgcgag cgctatccgt tccccgggac
     2641 gccgatcaag ctcgagttca aggcggcgaa ggattgaacg gcatgcccgc cacgctcatc
     2701 gcgttcgcga tcgtggcgct cgtcggctat ctcgtgggct ccatctcgag cggctacatc
     2761 gtcggccgcg tttaccgcaa cgtcgacctg cgcaccgtgg gatcgggctc gaccggggca
     2821 acgaacacgt tccgcacgct cggccgcggc gcgggcgtgc tcgtcgcgtc actggacatc
     2881 ctcaagggcg cgctcgcggt ggtgttcgcg cagatcgtgt tccccgaggg cagcgcggga
     2941 cggccgctgg ccgaggcgct cggcgcggtg tgcgcggtcg cggggcactg ctggccgctc
     3001 acgctgcgcg gccgcggcgg tcgtggcgtc gccacggggt tcggcgcgct gctcttcgtc
     3061 gccacgccgg cgtgggcgag cgcggtcgcg gcattcgcga tcgccatcgc gttcacgcgc
     3121 atggtctcgg tcggctcgct cgcgtcggtc atcggcgcgc tcgccggcta cctgctcttc
     3181 agcgcgacgg gctggctctc gttccattgg gcgacgctcg cgttcatcct cgtggccggc
     3241 acgatcgtct acgtgcgtca cctgccgaac atccagcgca tcgcgcgcgg cgtcgagccc
     3301 cggctcggct cgtgagcccg gtcagcgtcc ttcaggccgg gtcgtggggg acgacgctcg
     3361 cgacgatcct cgcgcgcgac gcgggccgag acgtcgtcct gtggacgcgc gacgccgagc
     3421 aggcgcgcgc catggccgcc gaacgcgaga accgccggta cctcccgggg atccgcatcc
     3481 ccgagcgcgt cgtgatcacc gcggatgtcg agcaggccct gtctcacgcg gaggacctga
     3541 tcgtcgcggc accgagcgtc ggcgtccgcg cgctgcgtga gcgcatcgcg ccgctgctcg
     3601 gcccgcgtca ccacatcctg tcggcgacga aggggctcga tggcgacggc cggcgcatga
     3661 gcgagctgtg ggccgaggtg gtgagccccg gtcgggtcgc ggtgctgtcc ggaccgaaca
     3721 tcagccgcga gatcgccgcg ggtcatcccg ccgcgagcgt ggtggcgtcg gtcgagccgg
     3781 agacggcgaa gcgcttccag acgatcgtcg ggacaccgat gttccgcgcg tataccgacg
     3841 cggatgtcgt cggcgtcgag ctctgcggag cactgaagaa catcgtcgcg ctcggcgcgg
     3901 gcgcgatcga cggcatggag tacggcgaca acgccaaggc cggcttcatg acgcgcggtc
     3961 tcgctgagat cgcgcgctac ggcttcgcgt gcggggcgaa cccgctcacc gtcgcgggac
     4021 tcgcgggcgt gggcgacctc atcgccacgt gcatgagcaa gcactcacgg aaccggacgg
     4081 tgggggagct cctggcgaag ggtgtgcggc tgcccgagat cagggagcgg cttggcgggc
     4141 agatcgccga aggactcacc acggcggagg ccacctaccg cgcggcgcgc gacaagagcg
     4201 tcagcatgcc gatcaccgaa cagacgcacc tcgtgctctc ggaaggcaag tccatccgcg
     4261 aggcgatgcg cgatctcatg cgccgcgaac gcggcgagga ggtagcggga ccgctcgcgg
     4321 agatctctcg gctcatcgcg agcgcggcgg cggcgcgtcg cggctgaccg cgttctcctt
     4381 ccgcgcctct ggcgttatgg tcacgagagt ccggtcgtga tcccttccat acgttccctg
     4441 cgatggctcg cgctcctcgt ggtcgcctgt tcggtgtcga acgcgccgct gccgccggct
     4501 ccggcgccca ccgtcgcacc cgaggtggca ctccgcgtcc tggtgacgcg ggcggagacg
     4561 ggcgcacctg tggagggcgc gcgcgtgtgc gccacgccca gccgtcgcgc cgagtcgtgc
     4621 gcgaccgccg gcaaggacgg cacggtggcg ttccgcggcg cgccgggaac gtacttcgtc
     4681 cgcgtgtcgg gtcccgccga gcagcgctac gtcgaagcgc agcgcgtggc cgacctctcg
     4741 aacggcgacg gcgcgctctg ggtcgagctg acgccccagc atcggatctc cggccgtgtc
     4801 cgcgacgcag acgcgaaacc cgtcgcgggc gccgaagcgt gcgcgcatcc gagcggcgac
     4861 gacccaccgg tctgcgcgcg cagtggtgcc gacggcgcgt acgtgatcga cgcgaaggcg
     4921 ggtgtctatc gtctcgaggt caccggcgcc cccggtgcgc ggctcgtgtc ccagtgggcg
     4981 cgcgggcgcg cgttcctcga ggaggccgac atcctcgacg cgcggacggc cgacgttccc
     5041 gatgtggacg tgacgctcgt gcgtggcgtg gtgctgcagg gcatcgtgcg gttcggcgga
     5101 gtgccggtgg aggacgcgca ggtgtgcatc cggacgctcg cggcgcccct accgtgggag
     5161 tgcgagcgca ccgacaagaa gggccactac gcggcgctgc gcgagccagg cgcgtactac
     5221 gtgtggaccg tgccgccggc cgacatccgc gccgtcccgc agtggtatga ccgcgcgctc
     5281 acgggagtcg gctccagcgt gctgcgcctc gacggcgacg cgacgctgga cgtcgcgctc
     5341 actacgggcc cgacgctcag tggcgtcctc cgcacggccg acggccagcc ggtgcggaac
     5401 gcgctcgtgt gcatcgacac ggcgttcgcc tctggccgca tctgccgcga gacggcgagc
     5461 acgggccgtt acagcatcac cacgcgaccc gagacgtaca tcgtcagcgt gatcccgccc
     5521 gcgcacagcg gactcattgg cgagtactgg aacgggaagc ggacctggaa ggacgccgac
     5581 gaagtgttcg tcggcggcga cgtgatcctc gatctcaccg tcgcgcgcgg cgcgacggtc
     5641 accggcaccg tgaaggacaa gcgcgggatc gccgtcgccg gtgcgacggt gaacctctgg
     5701 gacgaacgtg ggatcgcgga tgcgacggac accgacagtg ccggacgctt tgaggccgtg
     5761 gtgcgtccgg gcagcctgtc gctggaggtc ttcccgccat tcgtcgctaa cttggtcggc
     5821 aagacgcaga cggtcgacgt ggtgggcgac atggagctgc aggtcacgct ggacgacatc
     5881 gcaccctagc gggagcgctc gtgcggcgga gcgataaagt gagattgcgt ccggccatgt
     5941 gggatgcgcc gggcagactt caccgacgcc caaggagtga catccatgct tggccgactg
     6001 ctcaagccaa cgaccgacgc gtacgcgcac tgcgacatcc cgtgcggcat ctacgacccg
     6061 acgcccgcga agatcgccgc cgacaccgtc gcgaagatgg tcgagaagat cggtgcgctg
     6121 gacaagagcg cgacggactt cacgacgcgc ggcaatttcg tgcggatggt caccgtcaag
     6181 gagcagcacg cggagatctg caagaaggag cttcaggtgc tgtggtccga ctacttcaag
     6241 cccgagcatc tcgagaagtt cccgaagctg cacgacacgt tctggaaggc gctgaagctc
     6301 gcctcgaaga acaagcaaag cgttgacgcg caggcggccg cggatctcca ggcagcggtc
     6361 aaggagatca gcgacatgtt ctgggccacg aagaagtaga gatgaggcga gcgacgagcg
     6421 gtccggccgc gaggagcgag tccggaagcc gagtgacaac gaggcattga gcgggatgcg
     6481 gccgtggcgc gtggccgtgc gcggccacag catggagccg gcgctgcggg agggggactg
     6541 gctcctcgtc ctcccgccgc gccggatgcc ca
//
LOCUS       DASTZR010000100         3824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1233435, whole genome shotgun sequence.
ACCESSION   DASTZR010000100 DASTZR010000000
VERSION     DASTZR010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233435"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1534)
                     /locus_tag="VFV20_02275"
     CDS             complement(<1..1534)
                     /locus_tag="VFV20_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEU5287203.1"
                     /translation="MRPILEIRDLAVEFATREGIVHAVDGIDLAVPRGQTLGIVGESG
                     SGKSVTALSIMRLLDSPGRIARGEILLEGEDLVRKSERQLLDVRGKRIAMIFQEPVSS
                     LNPTVTVGEQLFEALRSDKYDAWRRGILPGLARSVKEIVRPNKERAAAARADAVDLLR
                     SVHLSSPEAVLRQYPFMLSGGMLQRIMITIALGSRPDILIADEPTTALDVTIQAQILQ
                     ILRERKAAADLTTVIITHDLGVIAEMCDRVAVMYAGRIVEEAAAKDLFHEPKHPYTRG
                     LLASMPSVERAIRARRPIEGSVPDLIGLRKESCFFAPRCPLATDICATERPPLASVAE
                     GKLVACHAYTHPVMKHLSGTIPALWEGGEPPAVAEPATADQMPTPLLAARNLKKHFPR
                     SRGLVRAVDGIDFDVWRGETFALVGESGCGKTTTGELLLRLQEPTEGDVQFEGRSIVH
                     ANGRELRGLRKRMQVVFQNPRSSLNPRMRVGAILSEPFDAHKDATLQAKASVEALLDM
                     VGL"
     gene            complement(1531..2397)
                     /locus_tag="VFV20_02280"
     CDS             complement(1531..2397)
                     /locus_tag="VFV20_02280"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5287204.1"
                     /translation="MTRRELDKAWELFKENRLVQIATGLFLLIVLVAIAAPLLPVADP
                     TQQNLSRVLRPPFWMTGADPSHPFGTDHLGRDLLARMVWGSRVSLTVGVGAVLVAALI
                     GVPLGIVAPYFGGKVDEAIMRAFDVVLSIPNLLIAIAVLLALGQSLLILILVLGFRST
                     VWYARTLRAKVLSIREEQYVKAARAVGLSDLRIMSRHILPNTLAPIIVLSTIYIGLMI
                     VVEAGLSFLGLTRIHVSWGFMVAESREYLATSWWTTTLPGLCIVVTVLSVNVIGDFLR
                     DVFDPRLQVGLE"
     gene            complement(2394..3380)
                     /locus_tag="VFV20_02285"
     CDS             complement(2394..3380)
                     /locus_tag="VFV20_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5287205.1"
                     /translation="MLRYVVRRTANIVIVLIGMTVFVFLLLHLAPGSPVYRLVGEQTT
                     PEEIVELERKFGLDQPLHVQYLRFVSGALQGDLGTSVIYSTSASDLLIERLPATLELT
                     LAATVLTLIVAIPTGIIISVRRKTWVDYAGTLFTIAGVSVPSFWLGLMLILIFSVDLG
                     WFAVSGKGPPLVDAIVDAVYLDFGTLGKALRHLVLPTITLSAFQLAFLSRLTRSSILE
                     ELGQNYVRAARARGLPRFLVITKHALRNALLPVLTVFGLELGSLIGGAVITEGVFAWP
                     GVGQLIFQAVSGRDYPLAQAGILIIGTFVVLLTFLVDLAYGFVDPRIRYATR"
     gene            complement(3397..>3824)
                     /locus_tag="VFV20_02290"
     CDS             complement(3397..>3824)
                     /locus_tag="VFV20_02290"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5287206.1"
                     /translation="ENFDPGDRILVYPLLEIARSAKAPVLFHSWYAPSEPALFWKLAN
                     DFPELPVIIGHMGGRLTIDAALIAERAPNVYLETSDHMYRLGTFAKRLGVERILFGTN
                     TPFAAPEAELFKVTVRKDLSREEKELILGGNALRVHGLS"
BASE COUNT          718 a         1221 c         1293 g          592 t
ORIGIN      
        1 tgagcccgac catgtccagc aaagcttcga ccgacgcctt tgcctggagc gtcgcatctt
       61 tgtgcgcgtc gaagggctcg gagaggatgg cgccgacacg catccgcggg ttgagggacg
      121 accgcggatt ctggaagacg acctgcatgc gcttccgcag tccccgcagc tcgcgcccgt
      181 tggcgtggac gatggagcgg ccctcgaact gcacgtcccc ttcggtgggc tcctgcagtc
      241 tcaagaggag ctcgccagtc gtggtcttgc cgcagccgct ctccccgacg agcgcgaagg
      301 tctcaccgcg ccagacgtcg aagtcgatcc cgtcgacggc gcggaccaga ccgcgcgacc
      361 gcgggaaatg cttcttgagg tttcgcgccg cgagcagggg cgtcggcatc tgatccgcgg
      421 tcgccggctc ggcgacggcg ggcggctcgc cgccctccca cagcgcgggg atcgtgccgg
      481 agagatgctt catcacggga tgcgtatacg cgtggcaggc gacgagcttc ccctccgcca
      541 cggatgcgag tggcggccgc tcggtggcgc agatgtcggt cgcgagcggg cagcgtggcg
      601 cgaagaagca cgattcctta cgcagaccga tcaggtcggg caccgagccc tcgatgggcc
      661 gccgcgcgcg gatcgcgcgc tcgaccgacg gcatcgaggc gagcagtccc cgggtgtatg
      721 ggtgcttcgg ctcgtggaac agatccttcg cggcggcctc ctcgacgatc cgcccggcat
      781 acatcaccgc gacccggtca cacatctccg cgatgacccc caggtcgtgc gtgatgatca
      841 ccgtggtgag atcggctgcc gcctttctct ctcgcaggat ctggaggatc tgtgcctgga
      901 tcgtcacgtc gagcgcggtc gtcggctcgt ctgcgatcag gatgtccggg cggctcccga
      961 gcgcgatcgt gatcatgatg cgctggagca tcccgccgct cagcatgaat gggtactggc
     1021 ggaggaccgc ctcaggcgag gagaggtgga cagaccgcaa cagatcgacc gcatccgcgc
     1081 gcgcggcagc ggcgcgctcc ttgttcggcc gcacgatctc cttgacggag cgcgcgagac
     1141 ccgggaggat tccgcgccgc caggcgtcgt acttgtcgga acgtagtgct tcgaacagct
     1201 gctcgcccac ggtcacggtg ggattcagag acgagaccgg ctcctggaag atcatcgcga
     1261 tgcgcttgcc gcggacgtcg aggagttgac gctcggactt ccgcacgaga tcctcgccct
     1321 ccagcaggat ctcgccgcgg gcgatccgcc cgggcgagtc gaggagccgc ataatcgata
     1381 gggcggtcac gctcttgccg gaaccggact cgccgacgat gccgagcgtc tgaccccgcg
     1441 ggaccgcgag gtcgatgccg tcgacagcgt gcacgatgcc ctcgcgcgtg gcgaactcta
     1501 ccgcgagatc ccggatctca aggatcggtc tcactcgagc cccacctgaa gccggggatc
     1561 gaagacgtcg cggaggaagt cgccgatcac gttcacgctc agcacggtga cgacgatgca
     1621 cagcccgggc agcgtggtag tccaccacga cgtggcgagg tactcccggc tctcggcgac
     1681 catgaagccc cacgagacgt ggattctcgt gaggcccagg aagctgagac cagcctccac
     1741 cacgatcatg agcccgatgt agatcgtgct cagcacgatg atcggcgcca gcgtgttcgg
     1801 gaggatgtgg cgggacatga tcctcagatc gctgagtccg acggcccgcg ccgccttgac
     1861 gtactgctcc tcgcggatgc tcaggacctt cgcgcgcagc gttcgtgcgt accacacggt
     1921 gctgcggaaa ccgagcacca ggatgaggat gaggaggctc tgccccagcg cgaggaggac
     1981 cgcgatggcg atgagcaggt tcgggatcga aaggaccacg tcaaacgcgc gcatgatcgc
     2041 ctcgtcgacc ttgcctccga agtacggtgc gacgatcccc agcggcaccc cgatgagcgc
     2101 cgcgacgagc acagcaccta cgccgaccgt gagcgacacg cgcgatcccc acaccatccg
     2161 cgcgagcagg tcgcgaccga gatggtccgt cccgaacggg tgggaggggt ccgcgcccgt
     2221 catccagaag ggtgggcgga gcacgcgcga gaggttctgc tgggtcggat cggcgaccgg
     2281 aagcagcggt gcggcgatgg cgacgaggac gatgaggagg aagagaccgg tcgcgatctg
     2341 gacgagacgg ttctccttga agagctccca tgccttgtcg agctcgcgtc tggtcatcgt
     2401 gttgcgtacc ggatccgagg atcgacaaag ccatacgcga ggtcgacgag gaaggtgagg
     2461 agcacaacga aggtcccgat gatcaggatg ccagcttgcg cgaggggata gtcacggccg
     2521 ctcacggcct gaaagatgag ctgtcccacg cctggccagg cgaacacgcc ctcggtgatc
     2581 accgcaccgc cgatgagcga tccgagctcg agaccgaaca cggtgaggac cgggaggagc
     2641 gcgttacgga gcgcgtgctt cgttatcacg aggaagcgtg gaaggccgcg cgcccgggcc
     2701 gccctgacgt agttctgacc cagctcctcg aggatcgagc tacgcgtcaa gcgagatagg
     2761 aacgcgagct ggaacgccga gagcgtgatg gtgggcagca cgagatgacg cagcgccttg
     2821 ccgagcgttc cgaagtccag gtagacggcg tcgacgatcg catcgaccag cggcggtccc
     2881 ttgcctgaga ccgcgaacca cccgagatca acggagaaga tcaggatgag catgagaccc
     2941 aaccagaagg acggcaccga gacgccggcg atcgtgaaga gcgtccccgc gtagtcaacc
     3001 caggtcttgc ggcggaccga gatgatgatc cccgtcggga tggcaacgat gagcgtgagc
     3061 accgtcgccg cgagcgtcag ctccagtgtc gccggaagac gctcgatgag cagatccgac
     3121 gcggaggtcg agtagatcac cgaggtaccg agatcgcctt gcagcgcccc actcacgaac
     3181 cggaggtact gcacgtggag tggctggtcc agcccgaact tgcgttccag ctcgacgatc
     3241 tcctcgggcg tggtctgttc gccgacgagc cggtacacgg ggctgcccgg agccagatgt
     3301 aggagcagaa agacgaagac cgtcatcccg atcaggacaa tgacgatgtt cgccgttcgt
     3361 cttacaacgt atcggagcac ggggcgcctg gccgatctag ctcaagccgt ggacgcgcaa
     3421 cgcgttgcct cctaggatca gctccttctc ctcccgactg agatctttgc ggacggtcac
     3481 cttgaagagc tcggcctccg gtgccgcgaa cggcgtgttg gtaccgaaca gaatgcgctc
     3541 aacaccgagc cgtttcgcga acgtgccaag gcggtacatg tggtcgctgg tctccagata
     3601 gacattcggc gcccgttccg caatcagggc cgcgtcgatc gtgaggcggc cgcccatgtg
     3661 tccgatgatc acaggtagct ctgggaagtc attcgccagc ttccagaaca gcgcaggctc
     3721 cgagggcgcg taccacgagt gaaacagcac cggagccttc gcggagcggg cgatctccag
     3781 caagggatac acgaggatcc ggtcacccgg atcgaagttc tctg
//
LOCUS       DASTZR010000101         2217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_424211, whole genome shotgun sequence.
ACCESSION   DASTZR010000101 DASTZR010000000
VERSION     DASTZR010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424211"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VFV20_02295"
     CDS             complement(<1..374)
                     /locus_tag="VFV20_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEU5287207.1"
                     /translation="MFSGKSEELIRRVKRAVIARRTVQVFKPLIDDRYGVEVVRSHDG
                     DSFVARPVRASGEILSLISPETTVVGIDEVQFFDPGIVDVVRALVASGRRVICAGLDL
                     DFRGEPFGPVPTLLALAERVDKL"
     gene            complement(456..695)
                     /gene="rpmE"
                     /locus_tag="VFV20_02300"
     CDS             complement(456..695)
                     /gene="rpmE"
                     /locus_tag="VFV20_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005768737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEU5287208.1"
                     /translation="MKEKIHPKYVQAKVTCACGNTFTTGSTKPELHVEVCAKCHPFFT
                     GKQHLLDVQGRVDRFNKRYAKSDAASATAEAAQSR"
     gene            complement(712..969)
                     /gene="rpmA"
                     /locus_tag="VFV20_02305"
     CDS             complement(712..969)
                     /gene="rpmA"
                     /locus_tag="VFV20_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU5287209.1"
                     /translation="MAHKKGAGSTRNGRDSAGQRLGVKEYAGVRVEAGTVLVRQRGTK
                     LLPGQNVGVGKDHTLFALVAGEVQYGPARDDRRKVSVIPQA"
     gene            complement(974..1291)
                     /gene="rplU"
                     /locus_tag="VFV20_02310"
     CDS             complement(974..1291)
                     /gene="rplU"
                     /locus_tag="VFV20_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEU5287210.1"
                     /translation="MYAVVRSGGKQYRVEEGSVVTVGKLTGNAGDTITLGEILFVADN
                     EAVKAGPAALKGAHITAEIVGHGKGPKIDVLRYKNKTRQRKTRGHRQDETTIRIKKIE
                     GLR"
     gene            complement(1557..>2217)
                     /locus_tag="VFV20_02315"
     CDS             complement(1557..>2217)
                     /locus_tag="VFV20_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287211.1"
                     /translation="GGGRRSVAGLIQVALLFVGGLILSFSLLLSAQLSPQLAQALPGV
                     ATLLTLAGIIIAAVRLARAALAVSWTSGGLRHMAIAVPWVIAAVLFQFFLVQQFIAAQ
                     GDITKVSIGLLHSLDHSYFVGMMTNVLFGAILVLTADDPKPWAWADNVVFWGLNIGAA
                     AFVTVLLTVGSGEGAKAFTHPVSFTAPIMGLAALLGIATLLMRLQAVTAGATVAGRIR
                     A"
BASE COUNT          344 a          757 c          766 g          350 t
ORIGIN      
        1 agcttgtcca cgcgctcggc gagcgcgagg agcgtcggta ccggaccaaa gggctcgccg
       61 cggaaatcga gatcgaggcc ggcgcagatc acgcgccgcc cgctcgcaac gagcgcacgg
      121 acgacgtcga cgatgccggg atcgaagaac tggacctcgt cgatccccac gaccgtcgtc
      181 tcgggtgaga tgaggctgag gatctcgccg ctcgcgcgga ccggacgcgc gacgaacgag
      241 tcgccgtcgt gcgatcgcac gacctcgacg ccatagcggt cgtcgatgag cggcttgaag
      301 acctgcaccg tgcggcgcgc gatcaccgcg cgcttcacgc ggcggatgag ctcctcgctc
      361 ttcccgctga acatgctgcc gacgacgacc tcgagccccg agtgcgtcgt cggtctgagc
      421 ccgcctggtc ctccggctgc gccgagaggg cccagctaac ggctctgggc ggcctccgcg
      481 gtcgcggatg ccgcgtcgga cttcgcgtat cgcttgttga agcggtccac gcggccctgg
      541 acgtcgagga ggtgctgctt gcccgtgaag aacgggtggc acttcgcgca gacctcgacg
      601 tggagctcgg gcttcgtcga gcccgtggtg aacgtgttgc cgcaggcgca ggtgaccttc
      661 gcctggacgt acttcgggtg gatcttctct ttcatgacct gctcctgctt cctatgcctg
      721 cgggatgacc gagaccttgc ggcggtcgtc gcgcgctgga ccgtactgca cctcgcccgc
      781 gacgagcgcg aacagggtgt ggtccttgcc gacgccgacg ttctggcccg ggagcagctt
      841 tgtgccgcgc tgccggacga gcaccgtgcc cgcctcgacg cggacgcccg cgtactcctt
      901 cacgccaagg cgctgcccgg cggaatcgcg accgttgcgg gtagagcccg cacctttctt
      961 gtgtgccatc taattacctc aggccctcga tcttcttgat gcggatggtc gtctcgtcct
     1021 ggcggtggcc gcgggtcttc cgctgccggg tcttgttctt gtaacggagg acgtcgatct
     1081 tcgggccctt gccgtggccg acgatctcgg cggtgatgtg cgcgcccttg agcgccgcgg
     1141 ggcccgcctt gacggcctcg ttgtccgcga cgaacaggat ctcgcccagc gtgatggtgt
     1201 cgccggcgtt gcccgtcagc ttgccgaccg tcacgaccga gccctcttcg acgcgatact
     1261 gcttcccgcc actgcggacg actgcgtaca aggaacgacc atccgaacag aaaaagtcgc
     1321 cgcaccgccc gtgcgacgta cgacaagtat agcgcgcgac ggtcggcgga cgacccgcgc
     1381 ggaggtcatc cgcggcagtc gtcgcgcgcg ggcggccacc gataccctct ctacgcgggg
     1441 tatcccgtcg ctcggtcgcg acgggaaccc tccccgcgcg ggggccctcg gtggccgccc
     1501 gctcactgtc gtcctctcct cggacctgcc ctcttccttg tcgtcctcgt ttcccgctac
     1561 gcgcggatgc gacccgcgac ggtcgcaccg gcggtgacgg cttggaggcg catcagtaag
     1621 gtggcgatgc cgaggagtgc ggcgagaccc atgatcggcg cggtgaagga cacgggatgc
     1681 gtgaacgcct tcgcaccctc accggagccg acggtcagca gcaccgtgac gaacgcggcg
     1741 gcgccgatgt tgaggcccca gaagaccacg ttgtcggccc aggcccacgg cttcggatcg
     1801 tccgcggtga gcacgaggat cgcgccgaac agcacgttgg tcatcatgcc gacgaagtac
     1861 gagtggtcga gcgagtggag caggccgatc gacaccttgg tgatgtcgcc ctgcgccgcg
     1921 atgaactgct gcacgaggaa gaactggaag agcaccgcgg cgatgaccca gggcaccgcg
     1981 atcgccatgt gacggaggcc gccgctggtc cacgacaccg cgagcgccgc gcgcgccaga
     2041 cggacggcgg cgatgatgat gccggcgagg gtgagcagcg tggcgacacc cgggagcgcc
     2101 tgcgcgagct gcggagagag ctgcgccgag agcagcaggc tgaacgagag gatcaggccg
     2161 ccaacgaaga gcagcgcgac ctggatgaga ccggcgacgc tgcgccggcc gccgccg
//
LOCUS       DASTZR010000102         3007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1181157, whole genome shotgun sequence.
ACCESSION   DASTZR010000102 DASTZR010000000
VERSION     DASTZR010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181157"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VFV20_02320"
     CDS             complement(<1..408)
                     /locus_tag="VFV20_02320"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEU5287212.1"
                     /translation="MIADVWARLRRENFPRIALLALAIFAGGALLETLLEGGPDGRIH
                     GLGDALWYAVVTMTSTGYGDIVPATAVGRIVGAVLMFGGVTVLSVVTATIASVLVAQR
                     IKEERGLETLKLKDHLLVCGWNAYTERVLDAVFA"
     gene            complement(464..1342)
                     /locus_tag="VFV20_02325"
     CDS             complement(464..1342)
                     /locus_tag="VFV20_02325"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEU5287213.1"
                     /translation="MTPRRGLFWPVLLIAIGLVFLLANFGYIGPLSVIALISLWPLLL
                     ILVGIDIAFARRWPYIALAAEVLLIAGGLVLVATQPTYPQWIAFGRAGSGPGESRVSA
                     PRGAAQSLSLHLNGGAGTFTVHGGATDLVEATSDQNTLSIRTSGTDRVDVRVDQADHG
                     VRFGPTAPVNLDARFASDIPTSLDVNAGAGEFVVDLRDTKVSDARINVGAASLRVVLP
                     RTVGDVSITVSAGASSVVIEVPDGVEARVTTSGALMSVRSENPRVASTETSGYASAKD
                     RVSVRVTAGASSVVIR"
     gene            complement(1339..1824)
                     /locus_tag="VFV20_02330"
     CDS             complement(1339..1824)
                     /locus_tag="VFV20_02330"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU5287214.1"
                     /translation="MSTRLERSTANRVVGGVCGGIAEYLQVDVTLVRVFFVVATFLTA
                     GLGLLAYIVLLVLMPLPGQPQPFVQGATAPAPPASAAAAEPMGEGSEPRTAPLTAPAP
                     VDPEATERRRAMFGYVLVALGAVFLLGNLGAFRIVRWDLIWPLVFVAAGVLLLAQRMR
                     R"
     gene            complement(1808..2212)
                     /locus_tag="VFV20_02335"
     CDS             complement(1808..2212)
                     /locus_tag="VFV20_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287215.1"
                     /translation="MNERDDLIDETVLRRSLRFEPDEQAPRFDVAAIAAMADAEHPWR
                     RTVLVALMAAAVTGAVAGTVWSTIAEHGAQIASVIANALLDGLIAIVTLLVPVAQAAS
                     EPVVPLSLLAALGVAIVHEFRERKENVHVHAS"
     gene            complement(2209..2763)
                     /locus_tag="VFV20_02340"
     CDS             complement(2209..2763)
                     /locus_tag="VFV20_02340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5287216.1"
                     /translation="MALFASFSVLPRALARPQRVTFDDAVRDHQDELYGVALRILGDR
                     DSAADATSRALLKAFRSWDRYDQTRPVRHWLLRIAANEAISIGRERTRERERHTSADD
                     ALDVADRAPLPDEHVVAREQRERVRLAVAALPELYRVPIVLRYFSDLTIDEIATVTDR
                     PASTVGVQLLRARAMLRAALEGTR"
     gene            complement(2793..>3007)
                     /locus_tag="VFV20_02345"
     CDS             complement(2793..>3007)
                     /locus_tag="VFV20_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEU5287217.1"
                     /translation="AVAGARDGVLLVRVTAPPAEGRANDAVVAVLARALDLAPSEVRI
                     ERGGSSRTKLVSLPAAAEARLRRLVK"
BASE COUNT          467 a         1067 c         1100 g          373 t
ORIGIN      
        1 cgcgaagacc gcgtccagga cgcgctcggt ataggcgttc catccgcaga cgagcaggtg
       61 atccttcagc ttcagcgtct ccaaccctcg ctcctccttg atccgctgcg cgaccagcac
      121 cgaggcgatc gtcgctgtga ccaccgagag gaccgtcacg cccccgaaca tgagcaccgc
      181 gccgacgatc cgccccaccg cggtcgccgg gacgatgtcg ccgtagcccg tcgacgtcat
      241 cgtcacgacc gcgtaccaaa gcgcgtcgcc caggccgtgg atgcgtccgt ccgggccgcc
      301 ctcgaggagg gtctccagga gcgccccgcc ggcgaagatc gcgagggcga ggagcgcgat
      361 gcgggggaag ttctcgcgcc ggagccgcgc ccagacgtcg gcgatcacgc gcgaagtatg
      421 gccgaacgac cgagcgctcg gcatgccctt ggctgtgcgg cgcttagcgg atgaccaccg
      481 agctcgcgcc cgcggtgaca cgcacgctga cgcggtcctt cgcgctcgcg tagccggagg
      541 tctcggtgga cgcgacgcgc gggttctcgc tccgcacgga catgagcgca ccgctcgtcg
      601 tgacccgggc ctcgacgccg tccgggacct cgatgacgac gcttgacgcc cccgcgctga
      661 cggtgatgga aacgtcaccc acggtccgcg ggaggacgac acgcagcgag gcggccccca
      721 cgttgatgcg cgcgtcgctg accttcgtgt cgcgcaggtc gacgacgaac tcgcccgcgc
      781 cggcgttgac atccagcgag gtcgggatgt cgctcgcgaa acgcgcgtcc aggttcaccg
      841 gcgcggtggg gccgaagcgg acgccgtggt ccgcctgatc gacgcgtacg tcgacgcggt
      901 cggtgcccga cgtgcggatg ctcagcgtgt tctgatcgga cgtggcctcc accaggtccg
      961 tcgcgccgcc gtgcaccgtg aacgtgccgg cgccgccgtt cagatggagt gacaggctct
     1021 gcgcggcgcc acgcggcgcg ctcacgcggc tctcacccgg gcccgagccg gcacggccga
     1081 aggcgatcca ctgcggatac gtcggctgcg tcgccacgag cacgaggcca cctgcgatga
     1141 gcagcacttc ggcggcgaga gcgatatagg gccaccggcg cgcgaacgcg atgtcgatgc
     1201 cgacgaggat gaggaggagc ggccacaggc tgatgagcgc gatcacggag agcggcccga
     1261 tgtacccgaa gttcgcgagg aggaagacga gcccgatcgc gatcagcagc accggccaga
     1321 agaggccgcg gcgcggggtc atcgccgcat ccgctgcgcg agcagaagga cgcccgcggc
     1381 gacgaacacc agcggccaga tgagatccca gcgcacgatg cggaacgcac cgaggttgcc
     1441 gagcaggaat acggcgccca gcgccacgag gacgtagccg aacatggcgc gccgacgctc
     1501 ggtcgcctcc gggtccaccg gcgcgggagc ggtgagcggt gccgtgcgcg gctccgaccc
     1561 ctcgcccatg ggctcggcgg ccgccgcgct cgccggcggc gccggcgcgg tggcgccctg
     1621 cacgaatggc tgcggctgcc cgggaagggg catcagcacg agcagcacga tgtacgcgag
     1681 cagcccgaga ccggcggtga ggaacgtcgc caccacgaag aagacacgca cgagtgtgac
     1741 gtccacctgg aggtactcag cgatcccgcc gcacacgccc ccgacgacgc ggttcgcggt
     1801 gctgcgctca agacgcgtgg acatgcacgt tctcctttcg ctcgcggaac tcgtggacga
     1861 tcgcgacgcc gagcgccgcg aggaggctga gggggacgac cggctcggag gccgcctggg
     1921 ccaccggaac gagcagcgtg acgatggcga tgaggccgtc gagcagcgcg ttcgcgatga
     1981 cggacgcgat ctgcgcgccg tgctccgcga tcgtcgacca cacggtcccg gcgacggcac
     2041 ccgtcaccgc ggccgccatc agcgccacca gcacggtgcg ccgccacgga tgctccgcgt
     2101 cggccatcgc cgcgatcgcg gcgacgtcga agcggggcgc ctgctcgtcg ggctcgaagc
     2161 gcagggatcg gcgcagcacg gtctcgtcga tgaggtcgtc acgctcgttc atcgcgttcc
     2221 ctccagggcg gcgcgcagca tcgcgcgggc gcggagcagc tggacgccga cggtcgaggc
     2281 cggccggtcg gtcaccgtgg cgatctcgtc gatcgtcaga tcgctgaagt accggaggac
     2341 gatcggcacg cgatagagct cggggagcgc cgccacggcg aggcgcacgc gctcccgctg
     2401 ctcgcgggcg acgacgtgct cgtcgggcag cggtgcgcgg tcggccacgt cgagcgcgtc
     2461 gtcggcgctg gtgtgccgct cacgctcgcg ggtgcgttcg cgtccgatcg agatcgcctc
     2521 gttcgcggct atgcggagca gccaatgacg gacggggcgg gtctggtcgt agcggtccca
     2581 ggagcggaag gccttgagca acgctcggct ggtcgcgtcg gcggccgagt cccggtcgcc
     2641 gaggatgcgc agggcgacgc cgtacagctc gtcctggtgg tcgcggacgg cgtcgtcgaa
     2701 cgtcacccgc tggggacgcg cgagggcacg cggcaggacg gagaagctcg caaagagcgc
     2761 cattcctccc atctacgccc gcggtcccga ccttatttca ccagacggcg caggcgggcc
     2821 tcggccgcgg ccggcaggct gaccagcttg gtccgggacg acccaccgcg ctcgatccgg
     2881 acctcagacg gcgcgaggtc gagggcccgc gcgagcaccg ccacgacggc gtcgttcgcg
     2941 cggccctcgg cgggcggcgc ggtcacgcgc acgagcagga cgccgtcacg cgctcccgcg
     3001 acggcgt
//
LOCUS       DASTZR010000103         3677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_814177, whole genome shotgun sequence.
ACCESSION   DASTZR010000103 DASTZR010000000
VERSION     DASTZR010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814177"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..1095
                     /locus_tag="VFV20_02350"
     CDS             181..1095
                     /locus_tag="VFV20_02350"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU5287218.1"
                     /translation="MRRPDATVLAVFVVVTIIGGSNFVAVRFSNRELPPFWGAGARFA
                     VAGLLLLAFSLWRRIPLPMGRPLLGVVAFGVLNFGVSYAFAYWGLLEAPAAVAATFVA
                     LVPLLTFFIATALRMERFRWTGLAGGMVAVLGVGVIFADQLRLTVPMSALLALFGQAL
                     AIAIATVLLKRLPRTHPIGTNAVAMLPGAAMLLALSAVAGERWTLPASTAVAAALVYL
                     VTVGGIGLFAGVVFIVSRWTASASAYITVLFPVVAVAVGALLAREMVSPQFYLGAGLV
                     MLGTYAGALIRPAPAPLGRDAVGRAAIP"
     gene            complement(1057..1653)
                     /locus_tag="VFV20_02355"
     CDS             complement(1057..1653)
                     /locus_tag="VFV20_02355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5287219.1"
                     /translation="MSRPDVDERITAHLAFARAVAARSLDPRCRGADREDLIAWGVFG
                     LVQAARRYRGDLGAPFGAYAARRVRGQVLDALRERDPLTRSERRAFREAQKISEDLPP
                     PYVEVSLDRLAELGEPTVPEDEVPRHHADPRWDRVASELRSLSALERRVVVDAFARGL
                     TLREIGKRVGLSESGVCRVRARALKKLRDRCAPDGIAA"
     gene            1836..2387
                     /locus_tag="VFV20_02360"
     CDS             1836..2387
                     /locus_tag="VFV20_02360"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU5287220.1"
                     /translation="MSDVPAAAERFFARYAYPDWLRQHSRVVGAIGAALAAARARAGD
                     RVDVESVTLAAYLHDIGRSPLLAGDPREHNELSAMILAAEGLAACVEPARRHAIYTVL
                     DPATAPRTLEEKLVYIADRRGGMAVEPLELRAQDTARRHPRFAEEIARAVPIAKSIER
                     DVFARLPFGPDELAARVTVEALR"
     gene            2384..3601
                     /locus_tag="VFV20_02365"
     CDS             2384..3601
                     /locus_tag="VFV20_02365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEU5287221.1"
                     /translation="MTDATRLQRRIDELAAIGGSGTSVTRLGLSAEEQRARELVASWC
                     APHTSASRRDAAGNLYVRFPGADASAPVVLVGSHIDSVPEGGRFDGALGVCCAVEAVI
                     SLVESGVPFARPVEVVAWADEEGARFGIGLFGSAAAFGKLPPNAAERRDKSGVSIAEA
                     LRAMGESGGLDLARRDPKEIRAYLELHIEQGPRLERAGVPLAIVSDIVGIYHGRVTVR
                     GRQDHAGATVMGARKDALVAASELIVALERIAAAVPDAVGTVGEIAVRPGAKNVVPGE
                     CVFSLDIRAPKRESIERVLREFGDAMSEVTRRRGVGLALDPLQSVAVTPLDPAVRDVL
                     VRATKAVGIDAPLLVSGAGHDAQNPALSGVPTGMLFVRSTGGSHTPKETADTADAVIG
                     TRALEDAIRELAC"
BASE COUNT          451 a         1335 c         1336 g          555 t
ORIGIN      
        1 tgttccgcat tcggtcgcgg aactgcgccg gcaggtcatc gagcgcgcgg cgcaccaggc
       61 gcgcgaatcg ggcgcgcctc aggaccgaca tgcgtgccgc ctcgctcggg tacgacgact
      121 catcggtcag agtgtcatcc gaagcgggac cggccgccat cgtcggttac gctgccgcga
      181 atgcgccgcc ccgatgccac cgtcctcgcc gttttcgtcg tcgtgacgat catcggcggc
      241 tcgaacttcg tcgcggtgcg cttcagcaac cgcgagctcc cgcccttctg gggcgccggc
      301 gcgcgattcg ctgtcgcggg actcctgctg ctcgccttct cgctgtggcg gcgcatcccg
      361 ctcccgatgg gccgtccgct gctgggcgtc gtcgcctttg gcgtcctcaa cttcggcgtg
      421 agctacgcgt tcgcctactg gggattgctc gaggcgcccg ccgccgtcgc ggcaacgttc
      481 gtggcgctcg tgccgctgct cacgttcttc atcgcgaccg cgctgcgaat ggagcgcttc
      541 cggtggaccg ggctcgcggg cggcatggtg gcggtcctcg gcgtcggtgt gatcttcgcc
      601 gaccagctgc gtctcaccgt gcccatgagc gcgctgctcg cgctcttcgg gcaggcgctc
      661 gcgatcgcca tcgcgaccgt gctcctgaag cgcctaccgc ggacacatcc gatcgggacg
      721 aacgcggtcg cgatgcttcc gggcgcggcg atgctcctcg cgctctccgc cgtcgcgggc
      781 gagcgatgga cgctgccggc gagcaccgcg gtcgccgctg ccctcgtcta tctcgtgacc
      841 gtcgggggca tcgggctgtt cgccggcgtc gtgttcatcg tgagccgctg gaccgcctcc
      901 gccagcgcct acatcaccgt cctctttccc gtggtcgctg tcgccgtcgg cgcgctcctc
      961 gcgcgtgaga tggtgagccc gcagttctac ctcggagccg gcctggtcat gctcggcacg
     1021 tacgcgggcg cgctcattcg gcccgcaccg gcgccattag gccgcgatgc cgtcgggcgc
     1081 gcagcgatcc cgtagcttct tcagcgcgcg cgcccgcacg cggcacacgc ccgactccga
     1141 gaggccgacg cgctttccga tctcgcgcag cgtcagcccg cgcgcgaagg cgtcgacgac
     1201 cacgcgtcgc tcgagcgcgc tgagcgaccg cagctcggac gcgacgcggt cccagcgcgg
     1261 gtcggcgtga tgtcgcggta cctcgtcctc cggcaccgtg ggctcgccga gctcggcgag
     1321 ccggtcgagc gacacctcga cgtacggcgg cggcaggtcc tcgctgatct tctgtgcctc
     1381 acggaacgca cgacgctcgc tgcgggtgag cggatcgcgt tcccggaggg cgtcgaggac
     1441 ctgaccgcgc acgcgcctcg cggcgtacgc gccgaagggc gcaccgaggt cgccccgata
     1501 gcgccgcgcc gcctggacca acccaaagac tccccatgcg atgaggtcct cgcgatccgc
     1561 gcctcggcac cgcggatcga gggagcgcgc ggcaacggcg cgcgcgaagg cgaggtgtgc
     1621 ggtgatgcgc tcgtcgacgt ccgggcggct catgtcgtcg tcctgctccc gcgcgcgaac
     1681 gtaggaatcg cgcgacgggg tgtcaacgcc ggtcgtgcgc ggttatccac cgttccgcga
     1741 gaggtacgga tttccgtacc tcgccgcagg tgccgccgca gggcgacgag ctcgtgatcc
     1801 cgacaggacg ccgcgaggtg cgcgatgatg cggccgtgag cgacgtccct gcggccgcgg
     1861 aacgattctt cgcgcgctac gcctatcccg actggctccg gcagcactcg cgggtggtcg
     1921 gggcgatcgg cgcggcgctc gccgcggcac gtgcgagggc cggcgaccgc gtcgacgtcg
     1981 agtcggtcac gctcgccgcg tacctccacg acatcgggcg gagcccgctc ctcgccggag
     2041 atccgcgcga gcacaacgag ctctccgcga tgatcctcgc cgccgagggc ctcgccgcgt
     2101 gcgtggagcc ggcgcggcgc cacgcgatct acaccgtgct cgaccccgcg accgcgcccc
     2161 ggacgctgga ggaaaagctc gtgtacatcg ccgaccggcg cggcggcatg gccgtcgagc
     2221 cgctggagct gcgtgcgcag gacaccgcgc ggcgccatcc gaggttcgcc gaggagatcg
     2281 cacgcgcggt cccgatcgcg aagtccatcg agcgtgacgt cttcgcgcgg ctgccgttcg
     2341 gaccggacga gctcgcggcg cgcgtgaccg tcgaggcgct ccgatgaccg atgcaacgcg
     2401 gctgcagcgc cgcatcgacg agctcgccgc gatcggcggg agcgggacgt cggtcactcg
     2461 cctcggcctg agcgccgagg agcagcgcgc ccgtgagctc gtcgcgtcct ggtgcgcgcc
     2521 gcacaccagt gcgagccgcc gcgacgcggc ggggaatctc tacgtccgct ttccgggcgc
     2581 ggacgcgtct gcgccggtcg tcctcgtcgg cagccacatc gactccgtgc cggagggcgg
     2641 ccggttcgat ggcgcgctcg gggtgtgctg cgccgtcgag gctgtcatct cgctcgtcga
     2701 gtcgggcgtg cccttcgccc gcccggtgga ggtcgtcgcc tgggccgacg aggagggcgc
     2761 gcgcttcggc atcggtctgt tcggctccgc cgccgcgttc ggaaagctcc cgccgaacgc
     2821 ggccgagcgc cgcgacaaga gcggcgtctc gatcgccgag gcgctccgcg cgatgggaga
     2881 gtcgggcggg ctcgaccttg cgcggcgcga tccgaaggag atccgcgcgt acctcgagct
     2941 gcacatcgag cagggaccgc gcctcgagcg ggccggcgtc cctctcgcga tcgtgagcga
     3001 catcgtcggc atctatcacg gccgcgtcac ggtgcgcgga cggcaggacc acgcgggcgc
     3061 gacggtgatg ggcgcgcgca aggatgcgct cgtggcggcg agcgagctca tcgtcgcgct
     3121 cgagcgcatc gcggcggcgg tcccggacgc cgtcggcacg gtgggggaga tcgccgtgcg
     3181 gccgggcgcg aagaacgtcg tgccgggcga gtgcgtgttc tcgctcgaca tccgggcgcc
     3241 gaagcgggaa tcgatcgagc gcgtcctgcg cgagttcggc gacgcgatga gcgaggtcac
     3301 gcgtcggcgc ggcgttggtc tcgccctcga tcccctgcag tcggtggcgg tcacgccgct
     3361 cgaccccgcc gtgcgcgacg tgctggtgcg cgcgacgaag gccgtcggca tcgacgcgcc
     3421 gctgctcgtc tcgggcgccg gccatgacgc ccagaacccc gcgctgtcgg gcgtcccgac
     3481 ggggatgctc ttcgtccgct ccaccggggg cagccacacg ccgaaggaga ccgcggatac
     3541 ggcggacgcg gtcatcggta cgcgagcgct cgaggacgcg atccgggagc tcgcctgctg
     3601 agcgcggctc gtcggtggcg ccctgcggcg ctatagtcct gccagttgac gtgaactggc
     3661 gaaggtaggc atccaag
//
LOCUS       DASTZR010000104         2961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_450392, whole genome shotgun sequence.
ACCESSION   DASTZR010000104 DASTZR010000000
VERSION     DASTZR010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450392"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VFV20_02370"
     CDS             complement(<1..603)
                     /locus_tag="VFV20_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006696735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5287222.1"
                     /translation="MSLAGRLPPPPTPVVRLRGITKTYRIGSEILVRALQGVDLDIYP
                     GEFVAIMGPSGSGKSTLMNILGALDAPDAGTYELAGEMVGKLSDDQLAAIRNKRIGFV
                     FQTFNLLPRLSAIENVELPLVYGGDRAGRRERAVAALGEVGLADRVHHRPSELSGGQQ
                     QRVAIARALLNDPDLILADEPTGNLDSRSSAEILAIFQRLN"
     gene            complement(600..2480)
                     /locus_tag="VFV20_02375"
     CDS             complement(600..2480)
                     /locus_tag="VFV20_02375"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5287223.1"
                     /translation="MSLRTFVATWPGRIAALLLVAATAGGATFVARSNSGTPKAELRT
                     TTITRGSVTQTVSVSGSVSASGQARLAFKTGGRLAEVYVSVGQAVTIGQPLAKLDTTD
                     LATAMSTAQQNLANAQASYQKQLLAASDTRQSLADTQRSTATDIANAQQTLARIKANY
                     TAARSNFGSLTNLAQSDATSLNTALGNLRAGIDTLLVYLGAPGLVVTNDVRSATNSLN
                     QAETALDNARAYVTSVLAPALNDFGVAQSALLQTAGAFDEAVAANADIAGFGATFQTQ
                     QFAYNIASSRMSSAIDTVTGPLSSVQTSITAAQTSLHSTTAMGDPALTEARSYVSTVL
                     TMVSQTTQPASTAKTRLTQAANPLSTMNDTIQSSYANAVQGVSAAQDRAATSVRQAQS
                     AVANIPFNLQSAQASVDNANNAVATAQSNLDAAVLTAPAAGIVASIANQIGEFVTGGN
                     TNNAFMVLTNTNAMVLHGTVGEADVSKLKLGQVANVTIDALSGQKMTGKVSSLDPVAT
                     ISQGVPVYGIDIAIDVPASSLKAGMSGTAAVILAAKQNVLLVPNTAIRTASGQRGVQV
                     LKGGEAVDTPVTFGLANDQFTEAVSGLSEGDVVVIPQARATTSAQPNRGPGGGGQQVI
                     FR"
     gene            complement(2477..>2961)
                     /locus_tag="VFV20_02380"
     CDS             complement(2477..>2961)
                     /locus_tag="VFV20_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287224.1"
                     /translation="VANTGVVSAYRASLGGWSAQLRVLLTGVAVLGLGFSATALAWVV
                     YLGYVATAARGGPFGVPAALQLGLAAFGVIVVILLAVLAVGFSATAWRIGDTLFRPRA
                     LARFGAALPSPVIALIGATLLYVVWQIPVVGGALFLAVIAYALGAVVSARLAGGGSSA
                     "
BASE COUNT          416 a         1012 c         1067 g          466 t
ORIGIN      
        1 gttcaggcgc tggaagatcg cgaggatctc ggcgctcgag cgcgagtcga ggttccccgt
       61 gggctcgtcg gcgaggatga ggtccgggtc gttgagcagc gcgcgcgcga tcgcgacgcg
      121 ctgctgctgg ccgccggaga gctcgctcgg ccggtgatgc acgcgatccg caagacccac
      181 ctcgccgagc gccgcgacgg cacgctcgcg gcgtccggcg cggtccccgc cgtacaccag
      241 gggcagctcg acgttctcga tcgcgctcag acgggggagc aggttgaacg tctggaacac
      301 gaatccgatg cgtttgttgc ggatcgccgc gagctggtcg tcgctcagct tgccgaccat
      361 ctcgcccgcg agctcgtacg tcccggcatc gggggcgtcg agcgccccga ggatgttcat
      421 gagcgtcgac ttgccggaac ccgagggtcc catgatcgcg acgaactcgc cggggtagat
      481 gtcgagatcc acaccctgga gcgcgcgaac gaggatctcg ctgccgatgc ggtacgtctt
      541 cgtgatgccg cgcagccgca ccaccggcgt cggcgggggc ggcagacgac ccgcgaggct
      601 caccggaaga tgacctgctg accgccgccg cccgggccgc ggttcggctg cgcgctggtc
      661 gtcgctcgtg cctgcgggat gacgacgacg tcgccctcgg aaaggccgct caccgcctcg
      721 gtgaattgat cgttcgcgag tccgaacgtc accggcgtgt ccaccgcctc gccgcccttg
      781 aggacctgca ccccgcgctg acccgaggcc gtgcggatgg cggtgttcgg gacgagcagc
      841 acgttctgct ttgccgcgag gatcaccgcg gccgtgccgc tcatccccgc cttcagcgac
      901 gaggccggca cgtcgatggc gatgtcgatg ccgtacacgg ggacgccctg ggagatcgtc
      961 gcgaccggat cgagggagct gaccttgccg gtcatcttct gcccgctcag cgcgtcgatc
     1021 gtgacgttcg cgacctggcc gagcttcagc ttcgacacgt cggcctcgcc cacggtgccg
     1081 tgcagcacca tcgcgttggt gttcgtgagg accatgaacg cgttgttggt gttcccgccc
     1141 gtaacgaact cgccgatctg gttcgcgatg gacgcgacga tcccggcggc cggcgcggtg
     1201 aggaccgccg cgtcgaggtt cgactgtgcc gtcgccaccg cgttgttcgc gttgtcgacg
     1261 ctcgcctgtg cggactgcag gttgaacggg atgttcgcca cggcgctctg cgcctgccgg
     1321 acgctcgtgg ccgcgcgatc ctgcgcggcg gacactccct ggaccgcgtt ggcgtagctc
     1381 gactggatcg tgtcgttcat cgtcgacagc gggttcgcgg cctgcgtgag gcgcgtcttc
     1441 gcggtcgagg ccggctgcgt cgtctggctc accatcgtga gcaccgtcga gacgtacgac
     1501 cgcgcctcgg tgagtgccgg gtcccccatg gccgtcgtgc tgtggaggct ggtctgcgcc
     1561 gcggtgatgc tggtctggac ggagctcagc ggacccgtga cggtgtcgat cgcgctcgac
     1621 atgcgcgacg aggcgatgtt gtaggcgaac tgttgcgtct ggaacgtcgc accgaagccg
     1681 gcgatatccg cgttcgcggc gacggcctcg tcgaaggccc cagcggtctg gagcagagcg
     1741 ctctgggcga cgccgaagtc gttcagcgcc ggcgccagca cgctcgtcac gtatgcccga
     1801 gcgttgtcga gcgccgtctc ggcctggttg agcgagttgg tcgcggagcg cacgtcattg
     1861 gtcaccacga ggccgggcgc gccgagatag accagcagcg tgtcgatgcc ggccctgagg
     1921 ttcccgagcg ccgtgttgag cgacgtggcg tcgctctgcg cgagattcgt gagcgatccg
     1981 aaattggagc gcgccgcggt gtagttcgcc ttgatccgcg cgagcgtctg ctgagcgttc
     2041 gcgatgtccg tcgcggtgga ccgctgcgta tccgcgagcg actgcctggt gtcgctcgcg
     2101 gcgaggagct gcttctggta gctcgcctgc gcgttggcga ggttctgctg cgcggtcgac
     2161 atcgccgtgg cgagatcggt ggtgtcgagc ttcgcgagcg gctgcccgat cgtgacggcc
     2221 tggccgacgc tcacgtacac ctcggcgagc cgtccgccgg tcttgaatgc gaggcgcgcc
     2281 tgccccgacg cgctcaccga ccccgacacg ctgacggtct gcgtcacgct gccgcgcgtg
     2341 atggtggtcg tccgcagctc ggcctttggc gtcccgctgt tcgaccgcgc cacgaaggtc
     2401 gcgccacctg cggtcgctgc gaccagcagc agcgcggcga tgcggcccgg ccatgtggcg
     2461 acgaatgtcc gaaggctcat gcgctcgacc ctccaccagc aagtcgcgcc gatacgacgg
     2521 cgccgagtgc gtacgcgatg accgcgagga acagggcccc tccgacgacg gggatctgcc
     2581 agacgacgta gagcagcgtc gcgccgatga gcgcgatgac cggcgacggc agcgccgcgc
     2641 cgaagcgcgc gagcgcgcgc ggccggaaga gcgtgtcgcc gatgcgccat gccgtggcgg
     2701 agaacccgac cgcgaggacc gcgagcagga tcacgacgat cacaccgaac gcggcgaggc
     2761 cgagctgcag tgccgccgga acgccgaacg gcccgccgcg ggcggccgtc gcgacgtacc
     2821 ccaggtacac gacccacgcg agcgccgtcg ccgagaagcc gaggccgagc acggcgacgc
     2881 cggtgaggag cacgcgcagc tgcgcgctcc agccccccag cgacgcgcgg tacgcggaca
     2941 cgacgcccgt gttggcgacg a
//
LOCUS       DASTZR010000105         4380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_868044, whole genome shotgun sequence.
ACCESSION   DASTZR010000105 DASTZR010000000
VERSION     DASTZR010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868044"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            273..1349
                     /locus_tag="VFV20_02385"
     CDS             273..1349
                     /locus_tag="VFV20_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004003324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5287225.1"
                     /translation="MTTRATRSRVTEKVERICADEPDARALRLRVLEALRDAVAFDAF
                     VWLVTDPVTAVGSAPVADVPCLPELPRLIRLKYLTPVNRWTALARREDPVALLDRETK
                     GDRSRSLVWRELLRAYAIGDVASVALADRHGCWGFLDLWRGETGARFDDDDARLLGEI
                     APRLTRAIRETQAHTFVAPAVTDRRALGPAVVLLDEDLTITARTPATDTWLELLLPTP
                     EGQAPVPASVYNVAARLLAAEQHVDEGRATARVHLVDGLWVTLRAARLDAPALSTSSG
                     IAVTIEEASPLDRLDIFTRAFAFTPREAELLGHLARGADTREVAERMFLSELTVQDYL
                     KSIFAKTADHSRRGLVSRALGAQL"
     gene            complement(1346..2692)
                     /locus_tag="VFV20_02390"
     CDS             complement(1346..2692)
                     /locus_tag="VFV20_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate phosphoribosyl transferase"
                     /protein_id="HEU5287226.1"
                     /translation="MIITERLRPEVFRLPIEKIRGGYKSDIYFARTKLILERDARRER
                     VTMQVFQKHPRAVIVGTDQTLALLHVGAGAYRDRARSERLLERYLEAERRLYAHWLAM
                     PREGWDEYAPIAREVYDLSHELATLWEPAWQDLRVESLYDGETADPAEPVMRIEGEYA
                     SFAHLETLYLGALAEGTRVATNTRDVVTAARGKPVIMFGARHEAHEAQASSGYAAYVG
                     GAIGVSTDEQGEWWGSRGLGTIPHALIAVYGGDTTVATLKFDEYINRATDTVGHLTAK
                     GTPEAHVNLTALVDFRNDVVNTSLGVAHALGQRLWGVRVDTSESLVDKAVLDELRQRD
                     GVAAQREVRGVTPRLVELLRTALDDAGYRHVRIVASGGFDAAKIRRFEELGVPVDVYG
                     VGSALVHGSGFDHTADIVRVNGRDLAKTGRAYIPSDRLHDVDWGALTAERTAGRKT"
     gene            complement(2695..3495)
                     /locus_tag="VFV20_02395"
     CDS             complement(2695..3495)
                     /locus_tag="VFV20_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5287227.1"
                     /translation="MDLGLSGKVALVAAASKGLGRAIAEEFAREGARVAMCSREEAAI
                     ASAAEDIVRRTKGDVLPIAADVSTKEGCETFVQTALAKYGAIDVLIVNAGGPPPGRFD
                     DLDDAAWEKAFDLTLMSAVRLTRLALPQMRKQKSGSIVYSTSTALRQPTQYLTLILSN
                     AVRVSVHGLAKTLSADLAKDGIRVNAVTPGRIATERLVQLDTDTARRQGTTPEAVRKR
                     YEDTMIPMGRYGTPEEYAAAVVFIASPRASYITGASLQVDGGMLPSLF"
     gene            complement(3521..4333)
                     /gene="uppP"
                     /locus_tag="VFV20_02400"
     CDS             complement(3521..4333)
                     /gene="uppP"
                     /locus_tag="VFV20_02400"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HEU5287228.1"
                     /translation="MNDLVAAAILGLVQGLTEFLPVSSTAHLILVAGALGLDPDRFGL
                     SFDVALHLGTALAVLLYFARTWIALAVDLVRGRWRLPLLVILGTIPAAIAGLLLEKTV
                     EETLRSPYWIVLGLVVGSIVFVIAERAATQRREMRGLALGDAIIMGAAQAIALLPGIS
                     RSGITISSGLLRDLTREDATRFSFLLATPVILGAGLKTLLDARRAQALFSEPDVLAVG
                     FIVSFLSGMAAVAFLIRFLRGHSLNWFVGYRLALAAIVLFTVLFGWPGSTPR"
BASE COUNT          669 a         1537 c         1532 g          642 t
ORIGIN      
        1 tcgaactcga gatcgatcat cacgtagagg ggctggccga ccggcctgct gatgacgtac
       61 cgccggacgc cggagcgctc ccgaccggcg ggatcgctgt cgaacgcggc cttccagccc
      121 gcgaagtcgt gtaccgggtg ctcgatgtgc agcgtgatca tgctcgtgcc tccttgagct
      181 gaatacgccg agcgtaggaa ggcagtggcg tcgcaggaat cccagatcgc tggggattcg
      241 cgcggcagga cccgcggcgt acgctcgcca tcgtgacgac gcgtgcgacg cgcagccgcg
      301 tcaccgagaa ggttgagcgc atctgcgccg acgagcccga tgcccgcgcc ctccggctgc
      361 gcgtgctcga agcgctgcgc gacgccgtcg cgttcgacgc tttcgtctgg ctggtcacgg
      421 atcccgtgac cgcggtcggc tccgcgccgg tcgccgacgt gccatgcctg cccgagctgc
      481 cgcggctcat ccgcctcaag tacctgacac ccgtcaatcg ctggacggcg ctcgcccgac
      541 gggaggatcc ggtcgcgctc ctcgaccgtg agacgaaggg tgacaggtcg cgaagcctcg
      601 tgtggcgcga gctgctccgc gcctacgcga tcggggacgt cgcatccgtt gccctcgcgg
      661 accgccacgg ctgctgggga ttcctcgatc tctggcgcgg cgagacgggc gcacggttcg
      721 acgacgacga cgcgcgactg ctcggcgaga tcgccccacg cctgacacgc gcgatccgcg
      781 agacccaggc gcacacgttc gtcgcacccg cggtgacgga ccggcgggcg ctcgggcccg
      841 cggtcgtgct tctcgatgag gatctgacca tcaccgcacg gacccccgcg accgacacgt
      901 ggctcgagct cctgctcccg acgccggagg ggcaagcccc ggtcccggcg agcgtctaca
      961 acgtcgcggc gcggctcctg gcagcggagc agcacgtcga cgaaggacgc gcgacggccc
     1021 gcgtgcatct ggtcgacggc ctctgggtca cgctgcgcgc ggcacgcctc gacgcgccgg
     1081 cgctctccac ctcaagcggg atcgcggtca ccatcgagga ggcgtcgccg ctcgatcggc
     1141 tcgacatctt cacgcgcgcg ttcgcgttca cgccgcgcga agcggagctg ctcgggcatc
     1201 tcgcccgcgg cgccgacacg cgagaggtcg ccgagcgcat gttcctgtcg gagctcacgg
     1261 tgcaggacta tctgaagtcg atcttcgcga agaccgccga ccacagccgt cgtgggctgg
     1321 tgtcccgggc tctcggcgcg cagctctagg tcttgcggcc tgccgtgcgc tccgcggtga
     1381 gcgctcccca atcgacatcg tgcagacggt cgctcgggat gtacgcgcgg ccggtcttcg
     1441 cgaggtcgcg gccgttcacg cggacgatgt cggcggtgtg gtcgaaaccg ctcccgtgca
     1501 cgagcgccga gccgacgccg tagacatcga ccggcacgcc gagctcctcg aagcgccgga
     1561 tcttcgcggc gtcgaagccg ccggatgcca cgatgcgcac gtggcggtac ccggcgtcgt
     1621 cgagcgccgt tcggagcagc tcgacgagac gcggcgtgac gccgcgcacc tcccgctgcg
     1681 cggcgacgcc gtcgcgctga cgcagctcgt cgagcacggc cttatcgacc agcgattcgc
     1741 tcgtgtccac gcgcacgccc cacaggcgct ggcccagcgc gtgcgcgacg ccgagcgacg
     1801 tgttcacgac gtcgttgcgg aagtccacca gcgccgtgag attcacgtgc gcttcgggcg
     1861 tgcccttcgc ggtgaggtgt cccaccgtgt cggtcgcgcg gttgatgtac tcgtcgaact
     1921 tcagcgtggc gacggtcgtg tcgccgccgt acacggcgat gagcgcgtgc gggatggtcc
     1981 cgagcccgcg tgagccccac cattcgccct gctcgtcggt ggacacgccg atggctccgc
     2041 ccacgtaggc ggcgtagccg gaactcgcct gcgcctcatg cgcctcgtgc cgtgctccga
     2101 acatgatcac cggctttcca cgcgcggcgg tcacgacgtc ccgcgtgttc gtcgcgacgc
     2161 gcgtgccctc ggcgagcgcg ccgagataca gggtctcgag atgagcgaac gacgcgtact
     2221 cgccctcgat gcgcatgacc ggctcggccg ggtcggcggt ctctccgtcg tagagcgatt
     2281 cgacgcgaag gtcctgccac gccggctccc agagcgtcgc gagctcgtgc gagaggtcgt
     2341 acacctcgcg cgcgatcggc gcgtactcgt cccatccctc ccgaggcatc gcgagccagt
     2401 gcgcgtacag gcgccgctcg gcctcgagat accgctcgag cagccgctcg gagcgcgcgc
     2461 gatcgcgata cgccccggcg ccgacgtgca gcagggcgag cgtctggtcg gtgccgacga
     2521 tgacggctct cggatgcttt tggaagacct gcatcgtgac gcgctcgcgg cgcgcgtcgc
     2581 gttcgaggat gagcttggtg cgcgcgaagt agatgtccga cttgtacccg ccgcggatct
     2641 tttcgatcgg caggcggaag acctcgggcc gcagacgctc cgtgatgatc acgactagaa
     2701 caggctcggc agcatgccgc cgtcgacctg gagcgacgcg ccggtgatgt acgacgcgcg
     2761 cggcgaagcg atgaacacga ccgcggccgc gtactcctcg ggcgtaccgt agcgacccat
     2821 cgggatcatc gtgtcctcgt accgcttgcg cacggcctcc ggcgtcgtgc cctgccgtct
     2881 tgccgtatcg gtgtcgagct gcacgagccg ctcggtcgcg atgcgcccgg gcgtgaccgc
     2941 gttgacgcgg atgccgtcct tcgcgaggtc cgcggacagc gtcttcgcga ggccgtgcac
     3001 cgagacgcgc accgcgttcg agaggatgag cgtcaggtac tgcgtcggct gtcgcagcgc
     3061 ggtggaggtg gagtacacga tcgatccact cttctgcttg cgcatctgcg gcagcgcgag
     3121 ccgcgtgagc cggaccgccg acatgagcgt gagatcgaag gccttctccc acgcggcatc
     3181 gtcgagatcg tcgaagcgcc ccggaggcgg accgccggcg ttcacgatga ggacgtcgat
     3241 cgcaccgtac ttcgcgagcg cggtctgaac gaaggtctcg cacccttcct tcgtcgagac
     3301 gtccgccgcg atcggcagga cgtcgccctt cgtcctgcgc acgatgtcct ccgcggccga
     3361 ggcgatcgca gcctcctcgc ggctgcacat cgcgaccctg gcgccctcgc gcgcgaattc
     3421 ctcggcgatg gcgcggccca gccccttcga cgcggcggcg acgagcgcga ccttacccga
     3481 gaggccgagg tccacatgga tagagtgcca cggtcgacgg ctagcgcggt gtcgagcccg
     3541 gccagccgaa caggaccgtg aacaggacga tcgccgcgag cgcgagccgg tagccgacga
     3601 accagttcaa ggagtggccg cgcaggaaac ggatcaggaa cgcgacggcg gccatacccg
     3661 acaggaacga cacgatgaag ccgacggcca ggacgtccgg ctcggagaag agcgcctgcg
     3721 cgcgtcgcgc atcgagcagc gtcttcagcc ccgccccgag gatcaccggt gtggcgagga
     3781 ggaacgagaa gcgtgtcgcg tcctcgcgcg tcaggtcccg cagcagcccg ctggagatcg
     3841 tgatccccga acggctgata ccgggcagga gtgcgatggc ctgcgccgcg cccatgatga
     3901 tcgcgtcgcc gagcgcgaga ccccgcatct cgcgccgttg cgtggcggca cgctcggcga
     3961 tcacgaagac gatcgagcca acgacgagcc cgagcacgat ccagtagggg gatcgcagcg
     4021 tctcctcgac ggtcttctcc agcagcaggc cggcgatggc cgcggggatc gtgccaagga
     4081 tgacgagcag ggggagccgc cagcgcccgc ggaccaggtc gacggcgaga gcgatccagg
     4141 tgcgcgcgaa atagaggagt acggcgagcg cggtcccgag atggagcgcg acgtcgaagg
     4201 agaggccgaa ccgatcgggg tcgaggccga gcgcgccggc caccaggatg agatgcgccg
     4261 tggagctcac cgggaggaac tcggtgaggc cctgcaccaa cccgaggatc gcggcggcga
     4321 cgagatcgtt catcgcatcg tcccttcggc cgtggcgagc gccccgtgcg cttcgcgctc
//
LOCUS       DASTZR010000106         9569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_552968, whole genome shotgun sequence.
ACCESSION   DASTZR010000106 DASTZR010000000
VERSION     DASTZR010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9569
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552968"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1379)
                     /locus_tag="VFV20_02405"
     CDS             complement(<1..1379)
                     /locus_tag="VFV20_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEU5287229.1"
                     /translation="MNSRWDAISPIDYRFVGGDTKLQKELGPYLSAAAVIRYQARVEG
                     ALALAMADAKILDHAIADEIARAADQVTPQEVADEEAKTRHDVRALVNVVRAHVSDEA
                     KRYVHLGATSYDIVDTANALRYRECVDRVIVPALARLVARCIALAEAEADTPQIGRTH
                     GRHAEPITFGFAVASYVSRIGDRTQALAAVARRLPGKLSGAVGAYNAMGLVASTSPRA
                     LEKRFLRSLGLDPRPMSSQIVEPEPWTDLAHACITALGVMANMADDMRHLQRTEIGEV
                     AESFAAEQVGSSTMPHKRNPVSFENVKSIWKAMAPRVITTYMDQISEHQRDLTNSASQ
                     RFLGEILASTAYAAGRLASSLEGIRVDRDRLKANLAMSRGAIVAEPLYVLLAKSGHPD
                     AHEAVRKLTIEAERSGRTVLEVAAQDVDLRGYLKKFTPAERRTLERPEEYLGIAPEVT
                     RSVTALWRE"
     gene            complement(1376..1855)
                     /gene="purE"
                     /locus_tag="VFV20_02410"
     CDS             complement(1376..1855)
                     /gene="purE"
                     /locus_tag="VFV20_02410"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008295247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEU5287230.1"
                     /translation="MSVLVGIVMGSRSDEPVMKAAEETLRGLGVACEMRVLSAHRTPD
                     ALRDWVVESEKRGARVFIAGAGAAAHLPGVVASITTRPVIGVPLIAPNAVANGLDALL
                     SIVQMPRGIPVATVALGEAGAANAGILAAEILAVADEALAGRVKMLREGMAAKVLAG"
     gene            complement(1959..3329)
                     /gene="purD"
                     /locus_tag="VFV20_02415"
     CDS             complement(1959..3329)
                     /gene="purD"
                     /locus_tag="VFV20_02415"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU5287231.1"
                     /translation="MRAIKDLFNEGEKIQRIIREQRANRSPGRSAGAPGLNCLVLGGG
                     GREYAIAWRLARSDSVATIDVVPGNPGMALFTRVLDFPVKDVGRLQQHLAAAQIDLAI
                     VGPDELVAEGVGDTLRRASVATVGPSREASRIEWSKTFAKQLMDEAGVATARWESFAT
                     TDDARAGLSRWDAPLVVKADGLAAGKGVTVCATREEARVAIGTPPTNSGAVLFEELLS
                     GEEASLQALVDGETVVALPPARDHKRVGDGDTGPNTGGMGAVSPTSVLPDEDAQRVAE
                     ETIAPIARALMARGTPYRGVIFAGLMRTAVGYRVLEYNARFGDPEAEVTLPRIGGDFA
                     RLLAALGEGRLAEYVREHPVRFSQRAFVDVVLCAEGYPGVPKTGARIDGLDRLPEGVY
                     AFHGATRRLADGSFATGGGRVIHIVAGGTNVAEARDRAYQGAQQVTFEGRFYRSDIAM
                     GEVVPA"
     gene            complement(3514..5115)
                     /gene="guaA"
                     /locus_tag="VFV20_02420"
     CDS             complement(3514..5115)
                     /gene="guaA"
                     /locus_tag="VFV20_02420"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEU5287232.1"
                     /translation="MSAPDARLHRTEGESAPQQRQLIAILDYGSQYTQLIARRVRESR
                     VYCEIFPHDVTPEELAERGVVGVILSGGPASVYETDAPGIDAKILDGRLPVLGICYGM
                     HLMAHALGGEVAPEGKREYGPATLEIADPSGLFEGLGQSERAWMSHGDTVKRMPAGFH
                     GIASTDRLPLAAMSDGRGRFAVQFHPEVMHTPRGAEVLRNFLYRVCGCTGDWTPAAFV
                     EEAVAELRRTIGDRHAICALSGGVDSAVAAALVAKAIGDRLTCVFIDHGLLRANEAEQ
                     VVATFGPRLRLVHVDASERFLAKLADVSDPERKRAIIGEEFIRCFEEQAKKIGLVGAD
                     ADKALLVQGTIYPDVIESKSRDSKTSARIKTHHNVGGLPEVMDLSVVEPLRRLFKDEV
                     RRVGAELGIPEDILWRHPFPGPGLAVRVIGAVTKERLDVLRAADAIFLEELRAADLYR
                     AIAQAFAVLTPVQSVGVMGDFRTYGWLVALRAVTTEDFMTADWARIPYDVLARISARI
                     VNEVPAVNRVVYDVSSKPPATIEWE"
     gene            complement(5123..6229)
                     /locus_tag="VFV20_02425"
     CDS             complement(5123..6229)
                     /locus_tag="VFV20_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003552815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEU5287233.1"
                     /translation="MTPEDKFGTEGLAFDDVLLIPARSDILPTQVSTRTFLTRELALD
                     IPILSAAMDTVTEARMAIALGRLGGMGVLHRNLTVDQQAAQVKECKAAGVRVGAAVGV
                     SGDADERVAALVAAGVDVVFVDTAHGHSGAVIRMVEKVKARHRVQVVAGNVATAAGTD
                     ELIAAGADGVKVGMGPGAICTTRVVAGAGMPQITAVFDAANAAAKAGVPVCADGGIQE
                     SGDIAKAIGAGAHTVMLGGLLAGVDESPGEVVDGPDGRFKSYRGMGSMGAMQARQTSR
                     DRYGQGDVAEFSKLVPEGIEGRVAARGALMPVIHQLVGGLRAGMGYVGAATIEDLRTK
                     ARFVRISGAGLRESHPHDVQITVEAPNYRVRTSG"
     gene            complement(6300..7409)
                     /locus_tag="VFV20_02430"
     CDS             complement(6300..7409)
                     /locus_tag="VFV20_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287234.1"
                     /translation="MERAHRLDPALGAVAVAGSVMYALASSIRAQGDRFVAIAFVVMV
                     TVGLAGIVSSETGASFTGASTNPGNTLTAMLVQPPASQGVPISSAGGVVSLSWTTTPT
                     APGAGHTLTYVVLRGPVGGPYTQITSVSGLSYTDTPPTDGTYQYVIQARVSGGGSFTS
                     QNSVAQSGISDRLAPSMSITCNGAACSGTAWYTAAVSVTVTGTDAGSGMGSVTRNVDG
                     AGQVSTTGASATFSVSGDSANHSVVYFGTDAVGNTSGSSTQTLKIDGTPPPAAALSGF
                     RFGTLQGDVTLTWTQSADATSGVATFDVYKKNISGSGAACGTTGFTVARTGLASGVTN
                     TVVNVGNNAWACFYLITKDNAGNQTQSNIVKVQGL"
     gene            complement(7349..7969)
                     /locus_tag="VFV20_02435"
     CDS             complement(7349..7969)
                     /locus_tag="VFV20_02435"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU5287235.1"
                     /translation="MTKTLGKILSLAAFGILFVLCLAFVSIRVLGFGTFIVTGGSMEP
                     NIHKGSLVLVQPVTSSEVKLGDVITFQQYGQTTTHRVISIGRNSAGLTFRTRGDANLT
                     ADPEDKWFPGQVGIVRATVPVAGYAAFAVQAYWRLAMTLIAAITFFACAGALVLRKES
                     GVRAPRPATLSSATRRPVMVTVDADDAWNAHIAWIQRSARSQSQAA"
     gene            complement(8047..8580)
                     /locus_tag="VFV20_02440"
     CDS             complement(8047..8580)
                     /locus_tag="VFV20_02440"
                     /inference="COORDINATES: protein motif:HMM:NF023806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TasA family protein"
                     /protein_id="HEU5287236.1"
                     /translation="MRKLNLLAGMALAGLVLLGLTATADKTSANFTATSTNPGNTFGS
                     ATLSMSNDKPNANDLINITNLVPGDTANRTVVITNSGNASFTYSAAISATATTLLWTD
                     VTNGIQVTVKRGATVLYTGPLKNMALAASGTIAAGATDTLTYDFSFPSTAGNTFQSLT
                     QDFTITYTATQLAGSAK"
     gene            complement(8815..9144)
                     /locus_tag="VFV20_02445"
     CDS             complement(8815..9144)
                     /locus_tag="VFV20_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287237.1"
                     /translation="MDALLDIAFPYRARKSFGVASFALVFLLVVAEIEALVLVALGVV
                     AHHLDLFLALQEAILPVSCFIALIAAVTFWWRARGWTKQRVEMLNLWDGGGSAFGERL
                     AKARQQQ"
     gene            complement(9208..>9569)
                     /locus_tag="VFV20_02450"
     CDS             complement(9208..>9569)
                     /locus_tag="VFV20_02450"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HEU5287238.1"
                     /translation="PRRVGWFDAVAARHAHRLNAFTEIAVTKLDVLGAYAEIPFCVAY
                     EREGKRTTDMPPTALLEGVTPVYERCAGWSVSLDGITDRAHLPAAARAYLEKIERTVG
                     APIGMVGIGPERSATLL"
BASE COUNT         1342 a         3356 c         3374 g         1497 t
ORIGIN      
        1 ttctcccgcc atagcgccgt taccgatcgc gtgacctcgg gggcgatgcc gaggtactcc
       61 tcgggacgct cgagcgtgcg ccgctccgcc ggcgtgaact tcttgaggta cccgcgcaga
      121 tcgacgtcct gcgccgcgac ctcgagcacc gtgcggccgc tgcgctcggc ctcgatggtc
      181 agcttgcgca cggcctcatg ggcgtcgggg tggccgctct tcgcgaggag cacgtagagc
      241 ggctcggcga cgatcgcccc gcgcgacatc gcgaggttcg ccttgaggcg gtcgcggtcc
      301 acgcggatgc cctcgaggga cgacgcgagg cggccggccg cgtacgcggt ggacgcgagg
      361 atctcgccga ggaagcgctg cgacgcggag ttggtgaggt cgcgctggtg ctcgctgatc
      421 tggtccatgt acgtggtgat cacgcgcggc gccatcgcct tccagatcga cttcacgttc
      481 tcgaaggaca cggggttgcg cttgtgcggc atcgtggacg acccgacctg ctcggcggcg
      541 aacgactccg cgacctcacc gatctcggtg cgctgcaggt gccgcatgtc gtcggccatg
      601 ttcgccatca cgccgagcgc ggtgatgcac gcatgcgcga ggtcggtcca cggctcgggt
      661 tcgacgatct gtgacgacat cggtcgcgga tcgaggccga gcgatctgag gaagcgcttc
      721 tcgagcgcgc gcgggctcgt ggacgcgacg agccccatgg cgttgtacgc gccgacggcg
      781 ccggagagct tgcccggcag gcggcgcgcc accgccgcga gcgcctgcgt gcgatcgccg
      841 atgcgcgaca cgtacgacgc caccgcgaag ccgaacgtga tcggctcggc gtgacggccg
      901 tgcgtgcgtc cgatctgcgg cgtgtcggcc tcggcctcgg cgagggcgat gcagcgcgcg
      961 accaggcgcg cgagcgcggg gacgatgacg cggtcgacgc actcgcggta gcgcagcgcg
     1021 ttcgccgtgt cgacgatgtc gtacgacgtg gcgccgaggt gcacgtagcg cttcgcctcg
     1081 tcggagacgt gcgcgcggac gacattcacg agggcgcgca cgtcgtgccg cgtcttcgcc
     1141 tcctcgtccg cgacctcctg cggcgtcacc tggtccgccg cgcgcgcgat ctcgtcggcg
     1201 atggcgtggt cgaggatctt cgcgtcggcc atcgcgagcg cgagcgcgcc ctcgacgcgc
     1261 gcctgatagc ggatgaccgc ggccgcggag aggtacggcc cgagctcctt ctgcagcttc
     1321 gtgtcgccgc ccacgaagcg gtaatcgatg gggctgatcg cgtcccacct gctgttcatc
     1381 cggccagcac cttcgcggcc atgccctcgc gcagcatctt cacgcggccg gcgagcgcct
     1441 cgtccgcgac cgcgaggatc tccgccgcga ggatgccggc gttcgcggcg ccggcctcgc
     1501 cgagcgccac cgtggcgacg gggatgccgc gtggcatctg cacgatcgag aggagcgcgt
     1561 cgaggccgtt cgcgaccgcg ttcggcgcga tgagcggtac gccgatcacc ggccgcgtcg
     1621 tgatcgaggc gacgacgccg ggcaggtgcg cggccgcgcc cgcgcccgcg atgaacacgc
     1681 gcgcgcccct cttctccgac tcgacgaccc agtcgcgcag ggcgtcgggc gtgcggtgcg
     1741 ccgagagcac gcgcatctcg caggcgaccc cgaggccgcg cagcgtctcc tcggccgcct
     1801 tcatcaccgg ctcgtccgag cggctgccca tgacgatgcc cacgagcaca ctcatcgtcg
     1861 cttcgcgccg attcgcaagg gactgttctt cgcaggggct ccgcccctgc caccccgggt
     1921 acgcccttca tccgagatca gcaggaacat cacgtaactc atgccgggac cacctcaccc
     1981 atcgcgatgt ccgaacgata gaacctgccc tcgaacgtga cctgctgcgc accctggtag
     2041 gcgcggtcac gtgcttcggc gacgttcgtc ccacccgcca cgatgtggat cacccggccg
     2101 cccccggtgg cgaacgagcc gtcggcaagc cgtcgggtcg cgccgtggaa ggcatacacg
     2161 ccctccggca gccgatcgag gccgtcgatg cgcgcaccgg tcttcggcac cccgggatag
     2221 ccctcggcgc agagcaccac gtcgacgaac gcgcgctggc tgaaacggac cggatgctcg
     2281 cggacgtatt ccgcgaggcg tccctcaccg agcgccgcca gaagccgcgc gaaatcgccg
     2341 ccgatgcgcg gcagcgtgac ctcggcctcg ggatcgccga agcgcgcgtt gtactcgagc
     2401 accctgtaac caacagcggt ccgcatgaga ccggcgaaga tcacgccgcg gtagggcgtg
     2461 ccgcgcgcca tcagcgcccg ggcgatcggc gcgatggtct cctcggcgac gcgctgcgcg
     2521 tcctcgtcgg gcaggacgct cgtcggcgac acggcgccca tgccgccggt gttcggcccg
     2581 gtgtcgccgt cgccgacgcg cttgtggtcg cgcgcgggcg gcagcgccac gacggtctcg
     2641 ccgtccacga gcgcctgcag gctggcctcc tcaccgctga gcagctcctc gaagaggacg
     2701 gcgccgctgt tcgtcggcgg ggtgccgatg gccacgcgcg cctcctcccg cgtcgcgcac
     2761 actgtgacgc ccttccccgc cgcgaggccg tcggccttca cgacaagggg cgcgtcccag
     2821 cgcgacagcc cggctcgcgc gtcgtcggtc gtcgcgaacg actcccaccg cgccgtcgcc
     2881 acaccggctt cgtccatgag ctgcttcgcg aaggtcttgc tccattcgat cctggacgcc
     2941 tcgcgcgacg ggccgaccgt ggcgacgctc gcgcgccgca gcgtgtcgcc gacaccctcg
     3001 gccaccagct cgtcggggcc gacgatcgcg aggtctatct gcgccgcggc gaggtgctgc
     3061 tgcagtcgtc cgacgtcctt caccggaaag tcgaggacac gcgtgaagag cgccatgccc
     3121 ggattgcccg ggaccacgtc gatcgtggcg acgctgtcgc tgcgcgcgag ccgccacgcg
     3181 atcgcgtact cgcgtccgcc gccgccgagc accaaacaat tgagaccggg tgcgccagct
     3241 gagcgaccgg gactcctatt ggcgcgctgt tcacggatga tgcgttggat cttctctccc
     3301 tcgttgaaga ggtcctttat cgcgcgcatg cctgcatcca ctcggcgccg cggcggccgt
     3361 ctagcgccgg gctcgtctct cgctcgaatc gctcccgcca gcacccggcg ggcggccgcg
     3421 cgacgctcct gagctggcgg tcgcgggtct cgctcgagcc gagcccgacg ctcccgtccg
     3481 ccgctctggc cgtgtggccg cttggcatgc ggctcattcc cactctatgg tcgcgggtgg
     3541 cttcgacgag acgtcgtaca ccacgcgatt cacggcgggc acctcgttca cgatccgcgc
     3601 gctgatgcgc gcgaggacgt cgtaggggat ccgcgcccag tcggcggtca tgaagtcctc
     3661 ggtggtcacg gcgcgcagcg cgacgagcca gccgtaggtg cggaagtcgc ccatcacgcc
     3721 gacgctctgc accggcgtga gcacggcgaa cgcctgcgcg atggcccggt acaggtccgc
     3781 cgcgcggagc tcctcgagga agatcgcgtc ggccgcgcgc agcacgtcca gacgctcctt
     3841 cgtcacggcg ccgatgacgc ggaccgcgag gccggggccg gggaagggat ggcgccagag
     3901 gatgtcctcg gggatgccga gctcggcgcc gacgcgccgc acctcgtcct tgaagaggcg
     3961 ccgcagcggc tcgacgaccg agaggtccat cacctcgggc agcccaccga cgttgtggtg
     4021 cgtcttgatg cgcgcgctcg tcttcgagtc gcggctcttg gactcgatga cgtcgggata
     4081 gatcgtgccc tgcacgagca gcgccttgtc ggcgtccgcg cccacgaggc cgatcttctt
     4141 cgcctgctcc tcgaagcagc ggatgaactc ctcgccgatg atcgcgcgct tgcgctccgg
     4201 atccgacacg tcggcgagct tcgcgaggaa tcgctccgac gcgtccacgt gcacgagacg
     4261 aagtcgggga ccgaacgtcg cgacgacctg ctcggcctcg ttcgcgcgca gcaggccgtg
     4321 atcgatgaac acgcaggtga gccggtcgcc gatcgccttc gcgacgagcg ccgccgcgac
     4381 ggcggagtcg acgccgcccg agagcgcgca gatggcgtga cggtcgccga tcgtgcgccg
     4441 cagctccgcg acggcctctt cgacgaacgc cgcgggcgtc cagtcgccgg tgcagccgca
     4501 cacgcggtac aggaagttgc gcagcacctc ggcgccgcgc ggggtgtgca tcacctcggg
     4561 atggaactgg acggcgaacc ggccacgacc gtcgctcatc gccgcgagcg gaaggcggtc
     4621 cgtgctcgcg atgccgtgga agcccgcggg catgcgcttc acggtgtcgc cgtggctcat
     4681 ccacgcgcgc tcgctctggc cgagcccctc gaagagcccc gagggatcgg cgatctcgag
     4741 cgtcgccggc ccgtattcgc gcttgccctc cggcgcgacc tcgccgccca gcgcgtgcgc
     4801 catgagatgc atgccgtagc agatgccgag gaccgggagg cgaccgtcga ggatcttcgc
     4861 atcgatgccc ggcgcgtccg tttcgtacac cgacgcggga ccgccggaga ggatcacgcc
     4921 gacgacgccg cgctccgcga gctcctcggg cgtcacgtcg tgcgggaaga tctcgcagta
     4981 gacgcgcgac tcgcggacgc ggcgcgcgat gagctgggtg tactggctgc cgtagtcgag
     5041 gatggcgatg agctggcgct gctgcggggc ggactccccc tccgtccggt ggaggcgggc
     5101 gtccggcgcg ctcacctatc gattatccgc tggtccgaac gcgatagttg ggcgcttcga
     5161 cggtgatctg cacgtcgtgc ggatgcgatt cgcgcaggcc cgcgcccgag atgcggacga
     5221 agcgcgcctt ggtgcgcaga tcctcgatcg tcgcggcgcc gacgtagccc atgccggcgc
     5281 gaagcccgcc gacgagctga tggatgaccg gcatgagcgc tccgcgcgcc gccacacgac
     5341 cctcgatgcc ctcgggtacc agcttgctga actcggcgac gtcaccctgg ccgtagcggt
     5401 cgcggctggt ctgccgcgcc tgcatcgcgc ccatcgatcc cattccgcgg tacgacttga
     5461 agcggccgtc gggaccgtcg acgacctcgc ccggcgactc gtcgacgccc gcgagcaggc
     5521 cgccgagcat caccgtgtgc gcgccggcgc cgatcgcctt cgcgatgtcg cccgactcct
     5581 ggatgccgcc gtcggcgcag accggaacgc ccgccttcgc ggcggcgttc gccgcgtcga
     5641 agacggcggt gatctgcggc atgcccgcgc ccgccacgac gcgcgtggtg cagatcgcgc
     5701 ccggacccat gccgaccttc acaccgtcgg cgccggcggc gatgagctcg tcggtgcccg
     5761 cggccgtcgc cacgttcccg gcgacgacct gcacgcggtg gcgagccttg accttctcga
     5821 ccatgcggat gaccgcgccc gagtggccgt gggccgtatc gacgaagacg acatcgacgc
     5881 ccgcggcgac gagcgccgcg acgcgctcgt ccgcgtcacc ggacacgccg accgcggcgc
     5941 cgacgcgcac gccggcggcc ttgcattcct tcacctgggc ggcctgctga tcgacggtga
     6001 ggttgcggtg gagcacgccc atcccgccga gacggccgag cgcgatggcc atgcgcgcct
     6061 cggtcacggt gtccatggcc gccgagagga tcgggatgtc gagggcgagc tcgcgggtga
     6121 ggaacgttct ggtggagacc tgggtcggga ggatgtcgct gcgggccggg atgaggagga
     6181 cgtcgtcgaa ggcaaggcct tccgtaccga acttgtcctc aggggtcaat ccgccgccct
     6241 cacccggtcc agtttacccg gcgggggcgc gccttgaagc gggtcgattt cgggtcgacc
     6301 tagaggccct ggaccttcac gatgttgctc tgggtctggt tgcccgcatt gtccttcgtg
     6361 atgaggtaga agcatgccca tgcgttgttg ccgacgttca cgaccgtgtt cgtgacgccg
     6421 gatgcaagac ccgtcctcgc gacggtgaag ccggtcgtgc cacaggccgc gccagacccc
     6481 gagatgttct tcttgtacac gtcgaacgtc gccacccccg aggtcgcgtc cgcggactgc
     6541 gtccacgtga gcgtgacgtc tccctgcagc gtcccaaagc ggaagccgga gagggccgct
     6601 gcgggcggcg gcgtgccgtc gatcttcagc gtctgcgtcg acgagccgct cgtgttgccc
     6661 accgcgtccg tcccgaaata gacgacgctg tggttcgcgc tgtcgccgct caccgagaag
     6721 gtcgcgctcg cgccggtcgt cgacacctgg ccggcaccat cgacattgcg cgtcactgag
     6781 cccatccccg agccggcgtc ggtccctgtg accgtcacgg acacggcggc ggtgtaccag
     6841 gccgtgccgc tgcaggccgc gccgttgcag gtgatcgaca tcgatggcgc gagtcggtcg
     6901 gagatgccac tctgcgcgac gctgttctgg ctggtgaacg agccgccacc gctcacgcgt
     6961 gcctggatga cgtactggta ggtaccgtca gtcggcggcg tatcggtgta ggagaggcct
     7021 gagacgctcg tgatctgcgt gtacggtccc cccaccgggc cacgcagcac gacgtaggtg
     7081 agcgtgtgac cggcgcccgg tgcggtcggt gtcgtggtcc aggagaggct cacgacacca
     7141 cccgcgctcg agatcggcac gccctgtgac gccggcggct gcacgagcat cgccgtcagg
     7201 gtgttgcccg gattcgtcga tgcaccggtg aagctcgccc cggtctcgga ggacacgatg
     7261 ccggcgagac cgacggtcac catcacgacg aatgcgatcg ccacgaagcg atcgccctgt
     7321 gcacggatgc tgctcgcgag cgcgtacatc acgctgcctg cgactgcgac cgcgccgagc
     7381 gctggatcca ggcgatgtgc gcgttccacg cgtcgtccgc gtccacggtg accatgaccg
     7441 gccggcgcgt cgcgctcgag agcgtggccg gccgcggcgc ccgcacgccc gactccttgc
     7501 ggaggacgag cgctccggcg caggcgaaga acgtgatcgc ggcgatgagc gtcatcgcga
     7561 ggcgccagta ggcctggacc gcgaacgcgg cgtagccggc gaccgggacg gtcgcgcgca
     7621 cgatgccgac ctgtccgggg aaccacttgt cctccgggtc ggcggtgagg ttcgcgtcgc
     7681 ctcgcgtacg gaaggtgagt ccggcggagt tacgaccgat cgagatgacc cggtgggtcg
     7741 tggtctggcc gtactgctgg aaggtgatga cgtccccgag cttcacctcc gacgatgtga
     7801 ccggctggac gagcacgagc gatcccttgt ggatgttcgg ctccatcgag ccgcccgtca
     7861 cgatgaaggt gccgaagccc aggacgcgga tcgagacgaa cgcgaggcag agcacgaaga
     7921 ggatcccgaa ggccgccagc gagaggatct tgccgagtgt cttcgtcatc ttcgtgctcc
     7981 cttctttctt cttggtaagc gaccggggcg atccgtccca gtgcggaccg ccccggtcgg
     8041 agagtgctac ttggccgagc cagccagctg cgtcgcggtg tacgtgatcg tgaagtcctg
     8101 cgtgaggctc tggaacgtgt tgccggccgt gctcgggaag ctgaagtcgt aggtcagcgt
     8161 gtccgtggcg cccgcggcga tcgtgccgga ggcggcgagt gccatgttct tcagcgggcc
     8221 ggtgtacagg accgtcgcgc cgcgcttgac ggtgacctgg atgccgttcg tcacgtcggt
     8281 ccagaggagc gtggtggccg tcgcgctgat cgcggcgctg tacgtgaagg acgcgttgcc
     8341 gctgttggtg atgaccacgg tgcggttcgc cgtgtcgccg gggacgaggt tcgtgatgtt
     8401 gatgaggtcg ttcgcgttcg gcttgtcgtt gctcatggag agggtcgccg agccgaaggt
     8461 gttgcccggg ttcgtgctcg tcgcggtgaa gttcgcgctg gtcttgtcgg cggtagcggt
     8521 gaggccgagc aggaccagac ccgcgagcgc catgccagcc aggaggttca gcttgcgcat
     8581 ctgcgttgtc cctttctctt tccgctccgc cggtctgtct tccatctcgg gtccctggtc
     8641 gggcggttcc ttgtgggtgt cactgtcgcc gctcgcggcc cgcgctccat cggcgtctag
     8701 ggggaggaaa ggtccttcgt tggtcgtagt gctcggcggt cacccgtcca tcgccgccgg
     8761 gtcgaagtcc cccgaaggga cgaggcgcct cgtccctgcg gacgaggccc gggcctattg
     8821 ctgctgccgc gccttcgcca ggcgctcgcc aaaggcgctg ccgccgccgt cccagaggtt
     8881 cagcatctcg acccgctgct tggtccagcc gcgggcacgc caccagaagg tgacggcggc
     8941 gatgagcgcg atgaagcacg agaccggcag gatcgcctcc tggagcgcga ggaagaggtc
     9001 gaggtggtgc gccacgacgc cgagtgccac gaggacgagt gcctcgatct cggcgacgac
     9061 gagcaggaag accagcgcga acgaggccac gccgaatgac ttcctcgcgc gataggggaa
     9121 tgcgatgtct aggagcgcgt ccatgcccgg cggactatcg gggttggcgg cggcgcgaac
     9181 aagtccccca gtgtgaggag gaccccctca cagcagcgtc gcgctgcgct cgggcccgat
     9241 gcccaccatg ccgatcggcg cgccgacggt gcgctcgatc ttctcgaggt acgcgcgcgc
     9301 ggcggcgggc agatgcgcgc ggtcggtgat cccgtcgagg gacacgctcc atcctgcgca
     9361 gcgctcgtac accggcgtga caccctcgag gagcgcggtg ggcggcatgt cggtcgtgcg
     9421 ctttccctcg cgctcgtagg ccacgcagaa cgggatctcc gcgtaggcgc cgagcacgtc
     9481 gagcttggtc acggcgatct cggtgaaggc gttcagccgg tgcgcgtgac gcgcggcgac
     9541 cgcgtcgaac caccccacgc gcctcggcc
//
LOCUS       DASTZR010000107         2019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_868185, whole genome shotgun sequence.
ACCESSION   DASTZR010000107 DASTZR010000000
VERSION     DASTZR010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868185"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1449
                     /locus_tag="VFV20_02455"
     CDS             <1..1449
                     /locus_tag="VFV20_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5287239.1"
                     /translation="AALEWLEQFLVERDVTAVVSSHDRWFLDRVTSRTLSIEDRTVVE
                     YRGSYTHYARQRAEREAAQMVASARQAEEIARTEEFIRRYGAGQRSKEARGRGKKLAR
                     VERIEAPTRAATHTWRLEAADLGSETVLETTALVAGHVAPVVRTGPLRIGRDARVAVL
                     GPNGAGKTTLVRTLVADLPPLEGYVSAAPMVRVAYLEQAQAELTGSATVLESLRTSSG
                     LSDQDARDLLARFLFRGDDVDRAVGVLSGGERARLALARLSVREANLLVLDEPTNHLD
                     LAAREQLERVLADFAGTLLFVSHDRYFVDKLATELWLIGDGVLRRWEGNWTSYQRARS
                     AGRDAPVVEYQVAPADRTGDGRSGIFATASGGAPRRRDNEERQRGSRGEAHKGAKSPE
                     AGRTRPVGRRTARASAVRRVEGLRALEARIAAMELQLRQLTEKVAQIAQSGNYMETRR
                     VGEEHAALERSLRELYDEWAKASEPGTDGAPE"
     gene            1446..>2019
                     /gene="coaE"
                     /locus_tag="VFV20_02460"
     CDS             1446..>2019
                     /gene="coaE"
                     /locus_tag="VFV20_02460"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875726.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU5287240.1"
                     /translation="MTARVIGLTGPIGAGKSTVAAMLRELGAKVLDADAIVHDEQSRG
                     TLGYSAIVQTFGTKILGPDKEIDRRKLADEVFADPNKLAKLERILHPRVVARILEARK
                     MLKEDDVLVIEAIKLLESGLRNVCDEIWVVVAPREEMLRRLEARGLGRAEAEMRLRSQ
                     RSEEEFRSAAGVVIENDADRERTRERVREAW"
BASE COUNT          312 a          690 c          756 g          261 t
ORIGIN      
        1 gccgcgctcg agtggctcga gcagttcctc gtcgagcgtg acgtcaccgc ggtcgtcagc
       61 agtcacgacc gctggttcct cgatcgggtc acctcgcgca cgctctccat cgaggaccgc
      121 accgtcgtgg agtaccgcgg ctcgtacacg cactacgcgc ggcagcgcgc ggagcgcgag
      181 gccgcgcaga tggtcgcgtc ggcgcggcag gcggaggaga tcgcgcgcac cgaggaattc
      241 atccgccgct acggcgccgg ccagcgctcg aaggaggcgc gcggccgcgg caagaagctc
      301 gcacgcgtcg aacgcatcga ggcgccgacc cgagccgcga cgcacacctg gcggttggag
      361 gcggccgacc tgggcagcga gaccgtcctc gagacgaccg cgctggtcgc cggtcacgtc
      421 gcgccggtcg tgcgcaccgg tccgctccgc atcgggcgcg acgcgcgcgt agctgtgctc
      481 ggccccaacg gcgcggggaa gaccacgctg gtgcgcacgc tcgtggcgga ccttccgccg
      541 ctcgaaggct acgtctcggc ggcaccgatg gtgcgcgtcg cgtacctcga gcaggcgcag
      601 gccgagctca ccggcagcgc cacggtgctc gagtccctgc gcacgtcgag cgggctgtcc
      661 gatcaggacg ctcgcgacct gctcgcgcgc ttccttttcc gcggcgacga cgtcgatcgc
      721 gcggtgggcg tgctctccgg cggcgagcgc gcgcgcctcg cgctcgcgcg cctgtcggtg
      781 cgcgaggcga acctgctcgt gctcgacgag cccaccaacc acctcgatct cgccgcgcgc
      841 gagcagctcg aacgcgtgct cgccgatttc gccggcacgc tcctgttcgt gagccacgac
      901 cgctacttcg tggacaagct cgccaccgag ctctggctga tcggcgatgg cgttctccga
      961 aggtgggaag gcaactggac ctcctatcag cgcgcgcggt cggcgggccg agacgcgccg
     1021 gtggtcgagt accaggtcgc gccagccgac cggacgggtg acggccggag tggcatcttc
     1081 gcgaccgcca gtgggggggc cccacgtcga agagacaacg aggaacgaca gcgaggaagt
     1141 cgtggggagg cccacaaggg agcgaagagc ccggaggccg gcaggacgcg tcctgtcggc
     1201 cgccgcacgg cgcgtgcgtc ggccgtccgg cgcgtcgagg gtctgcgcgc cctcgaagcg
     1261 cggatcgcgg ccatggagct gcagctgcgg cagctcaccg agaaggtcgc gcagatcgcg
     1321 caaagtggca actacatgga gacgcggcgc gtcggcgagg agcacgccgc gctggagcgc
     1381 tcgctccgcg agctgtacga cgagtgggcg aaggcgagcg agcccggcac cgacggggcg
     1441 ccggagtgac cgcgcgcgtc atcggcctca ccggtccgat cggggcgggg aagtcgaccg
     1501 tcgcggcgat gctgcgtgag ctcggcgcga aggtgctcga cgccgacgcc atcgtgcacg
     1561 acgagcagtc ccgcgggacg ctgggctaca gcgcgatcgt ccagaccttc ggcacgaaga
     1621 tcctcggccc ggacaaggag atcgaccggc ggaagctcgc ggatgaggtc ttcgccgatc
     1681 cgaacaagct cgcgaagctg gagcgcatcc tccatccgcg ggtcgtcgca cggatcctcg
     1741 aggcgcgcaa gatgttgaaa gaagacgacg tcctcgtgat cgaggcgatc aagctgctcg
     1801 agtcgggtct gcggaacgtg tgcgacgaga tctgggtcgt ggtcgcgcct cgcgaggaga
     1861 tgctgcgccg cctcgaggcg cgcggcctgg ggcgcgcgga ggcggagatg cggctgcgca
     1921 gccagcggtc tgaggaggag ttccgctccg cggccggcgt cgtcatcgag aacgacgccg
     1981 accgcgagcg gaccagagaa cgtgttcgcg aggcctggt
//
LOCUS       DASTZR010000108         6672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_424476, whole genome shotgun sequence.
ACCESSION   DASTZR010000108 DASTZR010000000
VERSION     DASTZR010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6672
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424476"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="VFV20_02465"
     CDS             complement(<1..745)
                     /locus_tag="VFV20_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287241.1"
                     /translation="MRTALAAAVLVLGACTAAPTVVTPASSASSAPVSSSPSSAPASL
                     RFDGDKAHEHLAYLADPARGGRFTGSAGFDDAARYVADRFREIGLEPLGDGGTYFQRY
                     PAQIVDLAATPALARTGAGGKTFTHRVDFTESVGGRAGSGAAEAAVAVVGGGAATNGQ
                     DDFAGVDVSGRIALVTGPTVQGYLENAYARGALGVLLVGNAGLKYSYLAQFLVPTIPV
                     LVVTEDAANDLLAGSGRTVAQVRDAVRARR"
     gene            complement(742..3126)
                     /locus_tag="VFV20_02470"
     CDS             complement(742..3126)
                     /locus_tag="VFV20_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU5287242.1"
                     /translation="MGARAGVAITWQLVVGAAIVIGWLALGIAAPLLTSVDPLKQTSL
                     LIVGTRAIPPPFEPGQYGYPLGSDNAGRDIWVEVMYGARVTLSIVFVVLFARLLIGTV
                     LGAVAGWSAGRTVDRVVSALTDAFAAFPTILFALLWIFAFDIRSGIAAFAIALAITGW
                     WGFGRATRSAVVALRGRPFLEAGRALGLSETALFVRHVLPNLLPILAVAAALEASAVL
                     LALGELGFLGIVVGGGFSIPLDDRGLGTTFVFSTAEWGAILAGGRFSVYNAPWIALVP
                     AAAFASAVFGFNMLGHGLRTLFDRTPVPLARILTWRAAAALAAVVVVVRLVTPLIGPA
                     AGYVPISRTFDAGRAQQHIAYFADPAREGRYSGSAGYAASAEYVADRFREIGLTPLGD
                     GGTFFQRFQQVSVRLADTPTLATLGDRAKTYTHRVDFTERVGGRAAGGTAEGAVVYVG
                     GGIQTSEYSDYAGTHPEGNIVLIAGPTQGDPIDTAIRHGARGVIFAQAADAAVGIIKF
                     STIASFERDTVPVVTVTEAVADELIAGSGKKIGDLRATLEERRRRAQERPSLQRTVPE
                     PLSFDTATRVRLTVPLGPVERVNTMNVVGMLPGTDAERAKKFLIIGGHLDGVGTDPNG
                     AVFPAANDNASGPAVTIEVARVLAANRSMLKNSFIFAVFSGEEEGLIGSEIFAQASTG
                     KPHGPPNVVAFLDLDMTGCCGKLAASDEVFALHDRLKSAADRLGYDLDYTPSVGGSDH
                     VTYLRRRVPAIMIGPTDLGPFHTTGDTVATIDAKQLRASGEIVLQAALEMGASE"
     gene            complement(3126..4049)
                     /locus_tag="VFV20_02475"
     CDS             complement(3126..4049)
                     /locus_tag="VFV20_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5287243.1"
                     /translation="MNSVAMRVLSRVLLVLFTFILIVVLAQLALVAASTRAQHIGIDE
                     VIRRAWEGTQDYFSSLLQGSLGEATTTSILGNRRRSMGDVLLDAYPKSMVLVGLAVIV
                     ATAFGVLVGTVAAATNSRRVRGALLGLTALAVSTPSFLLAMLLILLSATMVSRFGVRL
                     WPSFGFGWDEHLILPVIVLAARPFAQIASFAYAALDETLSSDYVRTARSKGLSERVVL
                     MRHALANAAVPILGAVAAALSIGLSTLPVVEVFFSWPGLGFALLQAIRRFDAATAGTL
                     LGALAVTIVIIRLALDAVAARFVRAATGAPA"
     gene            4098..5642
                     /gene="metG"
                     /locus_tag="VFV20_02480"
     CDS             4098..5642
                     /gene="metG"
                     /locus_tag="VFV20_02480"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU5287244.1"
                     /translation="MKFYVTTAIDYVNGTPHLGHAYEKTGADALARFHRQRGHEVFFL
                     TGTDENSDRNVEAAKAQGISTQAFVDRNTGEFQRLCTAYAISHDRFIRTSVDEDHKAG
                     VQEFVRRWIANGDVYSGTYEGLYCRGCEAFYEEGDLVDGRCPLHPTRPEYLQHVKEEN
                     YFFRLSKYEQALKDLYARDPEFAVPESRRNEVLGWLDRGLKDISVSRGRSLEWGIAWP
                     GEPKHTVYVWFDALINYVTGVGFGTDQPRFEKWWPCDVHVIGKDISRFHCIFWPAMLL
                     AAGVPLPKRVHVHGFLENSGARLSKSSGNVMDPFAFVEEWGVDAARYLLLREAPFDKD
                     SPISAESFAKRYNADLANDLGNLLSRVTTMVERYSGGRVPAPGGGDSERLVRERAERT
                     LREHDELASRLLFSEALAAVFALVEEANRHFQRTQPWQLAKDASRKADVDAALYAGLE
                     ALRLAGYLLYPYLPSTSARICEQLGVVTPERATWSEVGRWGGLKTGDPVRTGAALFPR
                     LERAVS"
     gene            5654..6478
                     /locus_tag="VFV20_02485"
     CDS             5654..6478
                     /locus_tag="VFV20_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU5287245.1"
                     /translation="MIDAHAHLTDPRFADDVDGVIHRASAAGIERVLTCGEDVASSER
                     AIELAKRHPTVRVGVGVHPHRAATWSDAVAARLRALASDPRVVAIGEIGVDLSGRSAP
                     RDAQERAFAAQLALASDLGLPVVVHVRDAGDLARRIVDDVPWGGSASGASAVRGMVHC
                     YSEGPGEVAEWLGRGFALSFAGTVTYPKNTTLRAAAAATPADALLAETDAPYLAPQRR
                     RGERNEPAFVLETLAALAEAQGIPPGVLAERIWANAGRLFGARWLGGTAQRGAAST"
BASE COUNT         1049 a         2351 c         2392 g          880 t
ORIGIN      
        1 cgcgacgcgc ccgcacggcg tcccgcacct gcgcgaccgt gcgcccggac ccggccagga
       61 ggtcgttcgc ggcgtcctcg gtgacgacga gcaccgggat cgtcggtacg aggaactgcg
      121 cgaggtacga gtacttgagt ccggcgttgc cgacgagcag cacgccaagc gcgccccggg
      181 cgtacgcgtt ctccaggtac ccctgcaccg tcggcccggt gaccagggcg atccgaccgc
      241 tgacgtccac gccggcgaag tcgtcctgac cgttggtcgc ggcgccgccg ccgacgaccg
      301 cgaccgccgc ctcggccgcg ccggaaccgg cgcggccgcc cacgctctcg gtgaagtcga
      361 cgcgatgggt gaaggtcttc ccgcccgcgc cggtgcgggc gagcgcgggc gtcgccgcga
      421 ggtccacgat ctgcgcgggg tagcgctgga agtaggtgcc gccgtccccg agcggctcga
      481 ggccgatctc gcggaatctg tcggccacgt atcgcgcggc atcgtcgaac cccgcggacc
      541 cggtgaagcg cccgccgcgc gccgggtcgg cgagatacgc gagatgctcg tgcgccttgt
      601 cgccgtcgaa gcggagggag gccggtgcgc tcgacgggga cgacgagacc ggcgcgctcg
      661 acgcgctcga cgcgggcgtg acgacggtcg gcgccgcggt gcacgcaccg aggacgagca
      721 cggccgcggc gagcgcggtc ctcactcgga tgcgcccatc tcgagcgccg cctgcagcac
      781 gatctccccc gacgcgcgca gctgcttcgc gtcgatggtc gcgaccgtgt ccccggtcgt
      841 gtggaacgga ccgagatccg tcgggccgat catgatcgcg ggcacgcggc gccgcaggta
      901 tgtcacgtgg tcgctgccgc cgaccgaggg cgtgtagtcg aggtcgtacc cgaggcggtc
      961 cgctgcgctc ttcagacggt cgtggagcgc gaacacctca tccgacgcgg caagcttgcc
     1021 gcagcagccg gtcatgtcga gatccaggaa cgcgacgacg ttgggcggcc cgtggggctt
     1081 gcccgtggag gcctgcgcga agatctccga tccgatcagc ccctcctcct ccccggagaa
     1141 caccgcgaag atgaacgagt tcttcagcat ggagcggttg gccgcgagca cgcgggccac
     1201 ctcgatggtg accgctggtc cggaggcgtt gtcgttcgcc gcggggaaca ccgcgccgtt
     1261 cggatccgtg ccgacaccgt cgagatggcc accgatgatc aggaacttct tcgctcgctc
     1321 cgcgtccgtg cccggcagca ttccgacgac gttcatcgtg ttcacgcgct ccaccgggcc
     1381 gagcggcacc gtgaggcgga cgcgggtcgc cgtatcgaac gagagcggtt cgggcacggt
     1441 gcgctggagg ctcggtcgct cctgcgcccg ccgccggcgc tcctcgagcg tcgcgcgcag
     1501 gtccccgatc ttcttccccg agcccgcgat gagctcgtcg gcgacggcct cggtcaccgt
     1561 cacgaccggg acggtgtcgc gctcgaacga ggcgatcgtg gagaacttga tgatcccgac
     1621 cgccgcgtcg gcggcctggg cgaagatcac gcccctcgcg ccatggcgga tggccgtgtc
     1681 gatggggtca ccctgcgtcg gtccggcgat gagcacgatg ttcccctcgg gatgggtgcc
     1741 cgcgtagtcg ctgtactcgg acgtctggat gccaccaccg acatacacga ccgcgccctc
     1801 cgcggtgcca ccggcggcac ggccgccgac gcgctcggtg aagtccacgc ggtgcgtgta
     1861 cgtcttcgca cggtccccga gcgtggcgag cgtcggcgtg tccgcaagcc ggacgctcac
     1921 ctgctggaag cgctggaaga acgtcccgcc gtccccgagc ggcgtgagcc cgatctcgcg
     1981 gaaacgatcg gcgacgtact cggcggacgc ggcgtagccg gccgatcccg agtagcgccc
     2041 ttcgcgcgcg gggtcggcga agtaggcgat gtgctgttgc gcacggcccg cgtcgaaggt
     2101 gcgcgagatg ggcacgtagc ccgccgcggg tccgatgagc ggtgtgacga ggcgaacgac
     2161 gacaacgacc gcggcgagcg cggccgcggc gcgccaggtg aggatccgcg ccagcggcac
     2221 gggcgtccgg tcgaacagcg tccgcaggcc gtggccgagc atgttgaacc cgaacaccgc
     2281 gctcgcgaac gccgcggcgg ggacgagcgc gatccagggc gcgttgtaca ccgagaaccg
     2341 tcctccggcg aggatggcgc cccactccgc ggtggagaac acgaacgtcg tcccgagccc
     2401 gcggtcgtcg agcgggatgg agaagcctcc gcccaccacg atcccgagga agccgagctc
     2461 gccgagcgcg aggaggaccg cgctcgcctc gagcgcggcg gccaccgcga ggatcgggag
     2521 caggttcggc agcacgtgcc gcacgaagag cgccgtctcg gagaggccga gcgcgcgtcc
     2581 cgcctcgagg aacggccgtc cgcgcagcgc gacgacggcg gagcgcgtcg cgcggccgaa
     2641 gccccaccag ccggtgatgg cgagcgcgat ggcgaaggcg gcgatccccg accggatgtc
     2701 gaaggcgaag atccagagca gggcgaagag gatggtcggg aacgccgcga aggcatcggt
     2761 cagcgccgag acgacccgat cgacggtgcg accggcggac catcccgcga cggcccccag
     2821 cacggtcccg atgagcaggc gcgcgaagag cacgacgaac acgatcgaca gcgtcacccg
     2881 cgcgccgtac atcacctcga cccagatgtc gcggcccgcg ttgtcggacc ccagcgggta
     2941 gccgtactga cccggctcga agggaggcgg gatcgcgcgc gtgcccacga tcagcagcga
     3001 ggtctgtttc agcggatcga cggaggtgag caggggcgcg gcgatgccca gcgccagcca
     3061 gccgatgacg atcgcggcgc ccacgacgag ctgccacgtg atcgcgacgc ccgccctggc
     3121 acccatcacg ccggggcacc cgtcgcggcg cgcacgaacc gcgcggcgac cgcgtccagc
     3181 gcgaggcgga tgatcacgat cgtgaccgcg agcgcgccga ggagcgttcc ggccgtcgcc
     3241 gcatcgaagc gccggatggc ctgcagcagc gcgaagccga gccccggcca cgagaagaac
     3301 acctcgacca cgggcagggt ggacaggccg atcgacagcg cggcggcgac cgcaccgagg
     3361 atcgggacgg ccgcgttcgc gagcgcgtgg cgcatgagca cgacccgctc ggagagcccc
     3421 ttggaccgcg ccgtgcgcac gtagtcgctc gacagggtct cgtcgagcgc ggcgtacgcg
     3481 aagctcgcga tctgcgcgaa cggccgcgcc gcgagcacga tcaccggaag gatgaggtgc
     3541 tcgtcccacc cgaagccgaa cgacggccag agccggacgc cgaaccggct gaccatcgtg
     3601 gccgatagca ggatgagcag catcgcgagc aggaaggacg gcgtcgagac cgcgagcgcg
     3661 gtgaggccga gcagtgcgcc acgcacgcgc cggctgttcg tcgccgccgc gaccgtaccc
     3721 acgaggacgc cgaaggcggt cgcgacgatc accgcgagcc cgacgagcac catgctcttc
     3781 ggatacgcgt cgagcagcac gtcgcccatg gatcgtcgcc ggttgcccag gatcgaggtc
     3841 gtcgtcgcct cgccgaggct gccctggagg aggctcgaga agtagtcctg ggtcccctcc
     3901 caggcccgcc ggatcacctc gtcgatgccg atgtgctggg cgcgcgtgct cgcggcgacc
     3961 agggcgagct gcgcgaggac cacgatgagg atgaaggtga agaggacgag cagcacgcgg
     4021 ctcaggaccc gcatcgcgac gctgttcacg ctgctggtgc ggcggccatg ccgacctaga
     4081 atacccacgc gaacagcttg aagttctacg tcacgacggc gatcgactac gtcaacggca
     4141 cgccccatct cggccatgcg tacgagaaga cgggcgcgga tgcgctcgcc cgttttcacc
     4201 ggcagcgcgg acacgaggtc ttcttcctga cgggtaccga cgagaacagt gatcgcaacg
     4261 tcgaggcggc gaaggcgcag gggatctcga cgcaggcgtt cgtcgaccgc aataccggag
     4321 agttccagcg cctctgcacc gcgtacgcga tctcgcacga ccgattcatc cggacgtccg
     4381 tggacgagga tcacaaggcg ggcgtgcagg aattcgttcg ccgctggatc gccaatggcg
     4441 acgtgtactc gggtacctat gagggtctgt actgccgcgg atgcgaagcc ttctacgagg
     4501 aaggggatct cgttgacggc cgctgtccgc tccacccgac gcggcccgag tacctccagc
     4561 acgtgaagga ggagaactac ttcttccgac tgtcgaagta cgagcaggcc ctgaaggatc
     4621 tctatgcccg cgatcccgag ttcgccgtgc ccgagtctcg ccggaacgag gtgctcggct
     4681 ggctggaccg cggcctgaag gacatcagcg tctcacgcgg ccgaagcctc gagtggggga
     4741 tcgcatggcc cggcgagccg aagcacaccg tgtacgtctg gttcgacgcg ctgatcaact
     4801 acgtcaccgg cgtgggcttc gggacggacc agccgcgatt cgagaagtgg tggccatgcg
     4861 acgttcacgt catcggcaag gacatcagcc ggttccactg catcttctgg ccggcgatgc
     4921 tgctcgcggc cggcgtcccg ttgcccaaac gcgtgcacgt ccatggcttc ctcgagaaca
     4981 gcggtgccag gctctccaag tcgagcggga acgtgatgga ccccttcgcc ttcgtcgagg
     5041 agtggggcgt cgacgccgcg cgctacctgc tcctgcgtga ggcgccgttc gacaaggact
     5101 cgcccatctc cgcggagtcg ttcgcgaagc gctacaacgc cgatctcgcg aacgatcttg
     5161 ggaacctgtt gtcgcgcgtg accacgatgg tcgaacgcta cagcggcggt cgcgtgccgg
     5221 cccccggcgg gggcgactcc gagcgtctgg tgcgcgagcg cgcggagcgc acgctgcgtg
     5281 agcacgacga gctcgcatct cgtctcctgt tctcggaagc gctcgcggcg gtcttcgcgc
     5341 tggtggagga ggcgaacagg cacttccagc gaacgcagcc gtggcagctg gcgaaggacg
     5401 cgtcgagaaa ggccgacgtc gatgcggcgc tctacgccgg cctcgaggcg ctgcgcctcg
     5461 ccggttacct gctgtatccg tacctgccga gcaccagcgc acgcatctgc gagcagctcg
     5521 gcgtcgtcac gcccgagcgc gcgacgtgga gcgaggtcgg gcgctggggt gggctcaaga
     5581 cgggcgatcc ggtgaggacc ggggccgcac tgttcccgcg tctcgaacgc gccgtcagct
     5641 gacgctgcgt ggcgtgatcg atgcccacgc gcacctgacc gatccgcgct tcgccgacga
     5701 cgtcgacggc gtgatccatc gcgcgagcgc ggccggcatc gaacgtgtcc tcacgtgcgg
     5761 cgaggacgtc gcctccagcg agcgcgcgat cgaacttgcc aagcgtcacc cgacggtgcg
     5821 cgtcggcgtc ggcgtgcacc cgcaccgcgc ggcgacctgg agcgacgcgg tcgcggcgcg
     5881 gctgcgcgcg ctcgcgtcgg atccgcgcgt cgtcgcgatc ggggagatcg gcgtcgacct
     5941 ctccggccgc agcgcgccgc gtgacgcgca ggagcgggcg ttcgcggcgc agctcgcgct
     6001 cgcgagcgac ctcggcctcc cggtcgtggt ccacgtccgc gacgcgggcg acctcgcgcg
     6061 ccggatcgtc gatgacgtcc cctggggcgg ctccgcgagc ggagcgagcg cggtccgcgg
     6121 gatggtgcac tgctacagcg agggtcccgg cgaggtcgcc gagtggctcg ggcgcggctt
     6181 cgccctgtcc ttcgcgggca cggtgacgta cccgaagaac accaccctcc gtgccgccgc
     6241 ggccgcgacg ccggccgatg cccttcttgc cgagaccgat gcgccgtacc tggcacccca
     6301 gcgccgccgc ggcgagcgca acgagcccgc cttcgtcctg gagacccttg ccgcgctggc
     6361 cgaagcgcag gggatcccgc cgggcgtcct cgccgagcgg atatgggcga acgccggacg
     6421 cctgttcggc gcgcgctggc ttgggggaac cgcccagcgc ggagcagcgt ctacctgact
     6481 ggaacaccca gaaagcaggt gacgcacatg cgccgtcgga ccgcgttcgg cctcctcgtc
     6541 ctggtcgtcc tcctcaccgc ggcatgtagc gcaagcacca ccggacccac agcgaccggc
     6601 ggctccggtg gactcgccat cgcgcccttc ccggcacccg cggccgacgc cgcaaagcgc
     6661 gaggccggaa ac
//
LOCUS       DASTZR010000109         2914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_30743, whole genome shotgun sequence.
ACCESSION   DASTZR010000109 DASTZR010000000
VERSION     DASTZR010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30743"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="VFV20_02490"
     CDS             complement(<1..844)
                     /locus_tag="VFV20_02490"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU5287246.1"
                     /translation="MSLEHEIAEQPAALARLLAEGDHQARALAKRLVAVRHLTFVARG
                     SSDNAATYGKYLFESVARIVAASAAPSLVTRYDAAPDFTGGAVIGISQSGSAPDVAAV
                     VAAARRDRALTIAITNGARSRLARAAHDTFLLRCGPERAIAATKTYTTSCLALAMLAS
                     ATAEARGERGISFAGLGDAAREAVACESDARRVARRIGSRRMLVVLGRGYDYAAALEG
                     ALKIKELARVWAEPYSTADFAHGPRTLLEPGTAVLLFSSRGRVSREAAAAAGQLHERG
                     ARVYA"
     gene            943..1494
                     /locus_tag="VFV20_02495"
     CDS             943..1494
                     /locus_tag="VFV20_02495"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HEU5287247.1"
                     /translation="MSDPVHWVLANVSRVDTRSRLAERVADHLRAQLPDDHIVLARYA
                     PRDRGDRIPVVIVGRERVFVIEPRDDDGALVCYQDHWYRRVGPGIAHPLGDAPSLRAR
                     RNRERVRSDLGTGGFITVHIEPLVLLTRGTPDDVRSSCVPVIAGIDALARHVVHGIAA
                     LPAPEHTRAVADALARNIKLASA"
     gene            complement(1527..2333)
                     /locus_tag="VFV20_02500"
     CDS             complement(1527..2333)
                     /locus_tag="VFV20_02500"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEU5287248.1"
                     /translation="MALTLATVLAHPDDETFGTGGTLIRYAREGVAVHSLCLTEGEKG
                     WAGGDGAPLVARENVGSTRALELAEAGRRMGLASVTCLKYPDGGLAGVNEEWVVRDIV
                     RWLRSVRPDVVITWGPDGGYGHADHIAAGRRALRAIDLAGIARHEPELGDHVHVKRAY
                     RFVVGAEFVDTLLRLESGFADYMKSLNVQLDRWTRERLGAAIDIADVLDAKIDAMDAH
                     RTQMPDINMWRRVRAKAPDLFREELYIREFPDPHGGLEADLFAALRPAGA"
     gene            2369..>2914
                     /locus_tag="VFV20_02505"
     CDS             2369..>2914
                     /locus_tag="VFV20_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5287249.1"
                     /translation="MSGTARIVVVEDDMSVAELVMLYLRNAGYIVEHAPTGARARALF
                     DEVRPGLVVLDLGLPDADGLDLLCELRALGDTPVIALTARAEDAQRIAGLDAGMDDYM
                     TKPFNPRELVSRVKAILRRSAGMPGGGHILTVGNVRVDPAHHEVRVDDTVVALRAKEF
                     ELLEAFCRQPGIVLTRDKLLED"
BASE COUNT          407 a         1058 c         1043 g          406 t
ORIGIN      
        1 ccgcgtacac gcgcgcgcca cgctcgtgga gctggccggc agccgcggcg gcctcacgcg
       61 acaccctgcc gcgcgacgag aagagcagca ccgccgtgcc gggctcgagg agcgtgcgcg
      121 ggccgtgcgc gaagtcagcc gtcgagtacg gttcggccca gacgcgcgcc agctccttga
      181 tcttgagcgc gccctcgagc gccgccgcgt aatcgtaccc gcggccgagc acgaccagca
      241 tccgccgaga gccgatgcga cgcgcgacgc ggcgggcatc gctctcgcat gcgaccgcct
      301 cccgggcggc gtcgccgaga ccggcgaagg agatgccgcg ctcgccgcga gcctccgccg
      361 tcgccgatgc gagcatcgcg agcgcgagac aggacgtcgt gtaggtcttc gtcgcggcga
      421 tggcgcgctc gggaccgcac cggagaagga atgtgtcgtg tgccgcgcgc gcgaggcgcg
      481 agcgggcccc gttcgtgatc gcgatggtga gcgcgcgatc gcgccgtgcc gccgccacga
      541 cggccgcgac gtcgggagcg ctgcccgatt gcgagatgcc gatgaccgcc ccgcccgtga
      601 agtcgggcgc ggcgtcgtag cgcgtgacga gcgagggggc ggcggatgcg gcgacgatgc
      661 gcgcgacgct ctcgaagagg tacttcccat aggtcgccgc gttgtcgctc gatccgcgcg
      721 cgacgaatgt gaggtgccgg acggcgacga gccgcttcgc cagcgcccgc gcctggtgat
      781 ccccttcggc cagcagacgc gccagcgccg cgggttgctc cgcgatctcg tgctcgagag
      841 acatggatgg atgatgcgcg accgggtacc agcccgtggt acgagggtcc ggcttgtacg
      901 gctgctcgcc agcgcgaacc atcgcgtctc ccgaggagca aggtgagcga tccggtgcat
      961 tgggtgctcg cgaacgtgtc gcgggtcgac acgcgaagcc gccttgccga gcgcgtcgcg
     1021 gaccacctgc gggcgcaact ccccgacgat cacatcgtcc tcgcacggta cgcgccgcgc
     1081 gaccggggcg accgcatccc ggtcgtcatc gtgggccgcg agcgcgtgtt cgtgatcgag
     1141 ccgcgcgatg acgacggcgc gctcgtctgc taccaggacc actggtatcg gcgcgtcggg
     1201 ccgggcatcg cgcacccgct gggcgacgcc ccgtccctgc gcgcgcgccg caatcgggaa
     1261 cgcgtgcgct cggacctcgg caccggcggc ttcatcaccg tgcacatcga gcccctcgtg
     1321 ctcctgacgc gggggacgcc cgatgacgtc cgcagctcgt gcgtcccggt gatcgccggc
     1381 atcgacgcgc tggcgcggca cgtggtgcac ggcatcgccg cgctgcccgc accggagcac
     1441 acgcgggccg tcgccgacgc tctcgcgcgc aacatcaagc tcgcctccgc ctgaccttgc
     1501 ggcggcgccg cgatgaacgg cggccgctac gcgccggccg ggcgcagtgc cgcgaagagg
     1561 tcggcctcca gccctccgtg cgggtctggg aactcgcgga tgtacagctc ctcgcggaag
     1621 agatcgggcg ccttcgcacg gacgcggcgc cacatgttga tgtcgggcat ctgcgtgcga
     1681 tgcgcatcca tcgcgtcgat cttcgcgtcg aggacatcgg cgatgtcgat cgcggcgcca
     1741 aggcgctccc gcgtccagcg atcgagctgg acgttcagcg atttcatgta gtcggcgaac
     1801 cctgattcga gccgcagcag cgtgtcgacg aactcggcgc cgaccacgaa ccggtacgcg
     1861 cgcttcacgt gcacgtgatc gccgagctcc ggctcgtggc gcgcgatgcc ggcgagatcg
     1921 atcgcgcgga gcgcgcgccg ccccgccgcg atgtggtccg cgtgtccgta gccgccgtcc
     1981 ggcccccagg tgatgaccac gtcgggccgc acgctgcgca gccagcggac gatgtcccgt
     2041 accacccact cctcgttgac acccgcgagg ccgccgtcgg ggtacttgag gcacgtgacg
     2101 gatgcgagcc ccatgcgccg acctgcttcg gccagctcga gcgcacgcgt gctgccgacg
     2161 ttctcgcgcg cgacgagcgg tgcgccatcg ccgcctgccc agcccttctc gccctccgtg
     2221 aggcacaggc tgtgaacggc gactccctcg cgcgcgtagc ggatcagcgt gccgccagta
     2281 ccgaacgtct cgtcgtcggg atgcgcgagc accgtcgcga gcgtgagcgc cacgcgggca
     2341 cgataacggg ccgccctatg ctcgcgtcgt gagcgggacc gcgcggatcg tcgtcgtcga
     2401 agacgacatg tcggtcgcgg agctggtcat gctgtatctc cgcaacgcgg gatacatcgt
     2461 cgagcacgcg ccgaccgggg cgcgcgcacg cgcgctgttc gacgaggtca ggccgggcct
     2521 ggtggtgctc gatctcggtc tgccggacgc cgacggcctc gatctgctgt gcgagctccg
     2581 cgccctgggc gacacgccgg tgatcgcgct caccgcccgc gccgaggacg cgcagcgcat
     2641 cgccgggctc gacgccggca tggacgacta catgacgaag ccgttcaatc cgcgcgagct
     2701 cgtctctcgg gtcaaggcca tcctccggcg gtcggcgggg atgccgggcg gcggccacat
     2761 cctgaccgtc gggaacgtgc gcgtcgaccc ggcgcaccac gaggtgcgcg tcgatgacac
     2821 ggtggtggcg ctgcgcgcga aggagttcga gctgctcgag gccttctgcc gacagcccgg
     2881 catcgtcctc acgcgcgaca agctcctcga ggac
//
LOCUS       DASTZR010000110         2222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1025126, whole genome shotgun sequence.
ACCESSION   DASTZR010000110 DASTZR010000000
VERSION     DASTZR010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025126"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1426)
                     /locus_tag="VFV20_02510"
     CDS             complement(<1..1426)
                     /locus_tag="VFV20_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287250.1"
                     /translation="MVELDALGRRIERIALEPETVRAPWTRLIRPILRTIGWRPAFEA
                     DALRALLDELRRRARTSDRSNGAPAREPDERSRLELTRLADEAVKELETNVVSAERAC
                     VVDGGVPVAYAAWLRRLFEVVVRAVRAARPERFDPAEARVAASIDRIMLLPPLAVAHA
                     AENPAAASGAEANPSPPATIEERTGSRLVELQLAAIDHLIDAARDEPQFLARRRRLLE
                     AARQMLLDTSAALPLDPAGVEARRSYLAAQIVRIDRLQAAGISPDVAVLHQARAALAS
                     GDRQRLHATLVAMEGGALATGDAAVASRVSEALVRLGGADRESKDVKAKSLARSQREL
                     LGGEVVGAVKRAYRNARDRLTPGVTPDEREISELARKYLAPGAELATLPLVLASDGCF
                     EVGGTLSPVRVTEHHVRARAVSYPTQDLVLLPARDPSDIPGAVIEDPRTVLTSLAEGR
                     LLTRKYVRYERVPRERTRMVGEVRV"
     gene            complement(1426..>2222)
                     /locus_tag="VFV20_02515"
     CDS             complement(1426..>2222)
                     /locus_tag="VFV20_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287251.1"
                     /translation="DAMALRITQLSGQPSTLKSRVVGPRIGALVGAIVERLDTHDRRS
                     FVARVEGLLAVLARAALARAIVPRFWLMWTASALARSDAATPPLPVFPPNIDGYRRLR
                     DAQRREPNAYTLAEIALRVAPAAVDGDGMSTVSTVLAEIPEPLRGAVLRDDLARIDRA
                     ISHLEAWWQALASSGSAEAPAEYLEKLVRSRFYRVLLDERAAERFSLEAKLIADLFPD
                     QLPAVSELRGRVDALLSRARDLALDLLRRRSDAAWTATLARGEKRD"
BASE COUNT          322 a          833 c          783 g          284 t
ORIGIN      
        1 acacgcggac ctcgccgacc atccgggtcc gctcgcgagg cacacgctcg tatcgcacgt
       61 acttccgggt gagcaggcga ccctccgcga gcgaggtgag caccgtccgc ggatcctcga
      121 tgaccgcgcc ggggatgtcg ctgggatccc gcgcgggcaa cagaacgaga tcctgggtgg
      181 gatagctcac cgcgcgcgct ctgacgtgat gctcggtcac gcgcaccggc gacagcgtcc
      241 caccgacctc gaagcacccg tcggaggcga gcaccagcgg cagggtggct agctcggcac
      301 cgggcgccag gtacttgcgg gcgagctccg agatctcgcg ctcgtccggg gtcaccccgg
      361 gcgtgaggcg atcgcgcgcg ttgcgatacg cgcgcttcac cgcgcccact acctcgccgc
      421 cgagcagctc gcgctgcgaa cgcgccaggc tcttggcctt cacgtccttg ctctcccggt
      481 cggcgccgcc gaggcgcacc agcgcctcgc tcacccggct cgccaccgct gcatcgccgg
      541 tggccagggc tccaccttcc atcgccacca gggtcgcgtg cagccgctgg cgatcgccgc
      601 tggccagcgc cgcgcgcgcc tggtgcaaca cggccacgtc gggcgagatg ccggcggcct
      661 ggagtcgatc gatgcggacg atctgcgctg cgaggtagct ccgccgcgcc tccacgccgg
      721 cgggatcgag cggcaacgcc gccgacgtgt cgagcagcat ctgtcgcgcg gcctcgagca
      781 accggcgacg gcgcgccagg aactggggtt cgtcgcgggc agcgtcgatc aggtgatcga
      841 tggcggcgag ctgcagctcc accaggcgcg atcctgtccg ctcctcgatg gtcgccggcg
      901 ggctcgggtt cgcttccgct ccgctcgcag cagccggatt ctccgccgcg tgcgccaccg
      961 ccagcggcgg caggagcatg atccggtcga tggacgctgc gacccgcgcc tccgccggat
     1021 cgaagcgctc cggccgtgct gcgcggaccg cgcgaacgac cacttcgaac aggcgcctca
     1081 gccacgccgc gtacgccacc ggcaccccac cgtcgaccac gcacgcgcgc tcggcggaaa
     1141 cgacgttcgt ctcgagctct ttcacggcct cgtcggcgag gcgcgtcagc tcgagtcgcg
     1201 agcgctcgtc aggctcccgt gccggagcgc cgttcgagcg gtcgctcgta cgcgcgcgac
     1261 gccggagctc gtcgagcaac gcgcgcaggg cgtccgcctc gaacgcgggc cgccagccga
     1321 tcgtccgcag gatgggacgt atcaaccgcg tccagggcgc ccttaccgtc tccggctcga
     1381 gcgcgatgcg ctcgatgcgg cgcccgagcg cgtcgagctc gaccatcagt cgcgcttctc
     1441 gccgcgagcc agcgtcgccg tccaggcggc gtcggagcga cgccgcagca gatcgagggc
     1501 gagatcgcgg gcacgagaga gcagagcatc gaccctgccc cggagctcgg acacggccgg
     1561 cagctggtcg ggaaacagat cggcgatgag cttcgcttcc agggagaagc gctcggcggc
     1621 gcgctcgtcg agcagcaccc ggtagaagcg cgagcgcacc agcttctcca ggtactccgc
     1681 cggcgcctcg gccgacccgc tcgaggccag cgcctgccac cacgcctcga ggtgcgagat
     1741 ggccctgtcg atgcgcgcca ggtcgtcgcg cagcaccgcg ccgcgcaagg gctccgggat
     1801 ctcggccagc accgtggaca ccgtcgacat gccgtcaccg tcgaccgctg cgggcgccac
     1861 acgcagggcg atctcggcca atgtgtacgc gttgggctcg cgccgctgcg cgtcgcgcag
     1921 ccgtcgatag ccatcgatgt tcggcggaaa gacggggagc ggcggcgtgg ccgcgtcgga
     1981 gcgcgcgagc gcggaggcgg tccacatcag ccagaagcgt ggcacgatgg cccgcgcgag
     2041 ggcggcgcgc gccagcaccg ccaggagccc ttcgacgcgc gcgacgaagg aacggcggtc
     2101 gtgggtgtcg agacgctcga cgatggcgcc caccagcgca ccgatgcgcg gccccacgac
     2161 ccgtgacttc agcgtcgacg gctgcccgct cagctgggtg atgcggagcg ccatcgcatc
     2221 ca
//
LOCUS       DASTZR010000111         2625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_998859, whole genome shotgun sequence.
ACCESSION   DASTZR010000111 DASTZR010000000
VERSION     DASTZR010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998859"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="VFV20_02520"
     CDS             complement(<1..972)
                     /locus_tag="VFV20_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU5287252.1"
                     /translation="MHDTRRGYDRRTFLKLAGATAAITACGGGGAVATATPTKAAATS
                     AAAATASAAPVTGTISVYSALNESTNNQFFGAFKTAFPGVTVNVLPLAAAGELQTRIR
                     TEKNSPKADIFIGGSSEFHDPLGKEGLLEAYQAPNGKDIDAKFKEATGLWTGWYTGIF
                     GFISNTDRLNKELGGKKPATWDDLLDPAWKGKLILPDPVKTGGGYIFIATQIFRFNRD
                     EAKAMDYMKKLHVNVAQYVPTSPGALTLIDQGQFVGCPNWAHDILTEKGKGNPVELNV
                     PSDTGFEVGAVSIVKGTKNLAAAQALVNWSLTKEAGELNVKLSNRGSV"
     gene            1075..1422
                     /locus_tag="VFV20_02525"
     CDS             1075..1422
                     /locus_tag="VFV20_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287253.1"
                     /translation="MAIDERDLALAREHPRGTERRTLLPYREALNDVAAYVRLPVADR
                     DVIVRWAEIRRRLAEDDGLDHDPANLADPLLPYALLRAHVLAGERAASGGRGGVDRAG
                     DIRAAVAAIRART"
     gene            complement(1419..1997)
                     /locus_tag="VFV20_02530"
     CDS             complement(1419..1997)
                     /locus_tag="VFV20_02530"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEU5287254.1"
                     /translation="MPDVLFRWSTAIGTLVLDTILLLLTLLVARGLPTREAFALRRPT
                     SWGEALRVGLLALGATWLTSLALELAVGHAVREQAVPQYSDPTRLAPFIANFVVIAVY
                     VPVVEESLCRGLGYGLLEAWGTPLAIVGSAVAFALAHGAVLDLPWVLVTGLGLAYLRS
                     RTGSLYPCVALHATVNGIAMVASVLIAAPPPV"
     gene            complement(2096..>2625)
                     /locus_tag="VFV20_02535"
     CDS             complement(2096..>2625)
                     /locus_tag="VFV20_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5287255.1"
                     /translation="TLKWLMGGPGIALLYARRAVADAAHPSAVGWWGVADPFAFDIEH
                     LEPGRGARRFEYGTPAVAAVYAARAGIELLREIGIATVRERHMLLSQRLVDGARAQGW
                     TVRCPTDARERTAIVTLEHPDPQRAVDALRASGVITDSRPGLIRLSPHYFNTPEEMDR
                     ALELLAPLRAAAATA"
BASE COUNT          383 a          928 c          946 g          368 t
ORIGIN      
        1 cacggagccg cggttgctga gcttcacgtt cagctcgccg gcctccttcg tcagcgacca
       61 gttcacgagc gcctgcgccg ccgcgaggtt cttcgtgccc ttcacgatgc tgaccgcgcc
      121 gacctcgaaa ccggtgtcgg acgggacgtt gagctcgacc gggttgccct tgcccttctc
      181 ggtcaggatg tcgtgcgccc agttcgggca gccgacgaac tggccctggt cgatgagcgt
      241 gagcgcgccc ggcgaggtcg ggacgtactg cgcgacgttc acgtgcagct tcttcatgta
      301 gtccatcgcc ttcgcctcgt ccctgttgaa gcggaagatc tgcgtggcga tgaagatgta
      361 gccgccgccg gtcttcaccg ggtcggggag gatgagcttg cccttccacg ccgggtcgag
      421 caggtcgtcc cacgtcgccg gcttcttccc gccgagctcc ttgttgaggc gatcggtgtt
      481 cgagatgaag ccgaagatgc ccgtgtacca gccggtccag aggcccgtgg cctccttgaa
      541 cttcgcgtcg atgtccttgc cgttcggcgc ctggtaggcc tcgagcaaac cctccttgcc
      601 gagcgggtcg tggaactcgc tcgacccgcc gatgaagatg tccgccttgg ggctgttctt
      661 ctcggtgcgg atgcgggtct gcagctcgcc cgccgcggcg agcggcagga cgttcaccgt
      721 cacgccgggg aacgcggtct tgaacgcgcc gaagaactgg ttgttcgtgg actcgttgag
      781 cgccgagtac accgagatgg tcccggtgac cggtgccgcg gacgcggtgg ccgccgccgc
      841 gctggtggcg gccgccttgg tcggcgtcgc ggtggcgacg gcgccgcctc cgccgcatgc
      901 ggtgatcgcc gcggtggcgc ccgcgagctt caggaaggtc ctgcggtcgt aaccgcgccg
      961 ggtgtcgtgc attcgttctc ctcccaaccc ccgcgtagcg ggtgcggcga ggagtctaga
     1021 ccgaccggac gaccaggcgg cgcggacgga ttcgcgcgtt cataggatgg cgacatggcg
     1081 atcgacgaac gcgacctcgc gctggcacgc gagcacccgc gcgggaccga gcgccgcacg
     1141 ctgctcccct atcgagaggc gctgaacgac gtggccgcgt acgtgcggct gcctgtcgcc
     1201 gaccgcgacg tcatcgtgcg ctgggccgag atccgccgcc gcctggcgga ggacgacggc
     1261 ctcgatcacg atcctgcgaa cctcgccgac ccgctcctcc cttacgccct gctgagagcg
     1321 cacgtgctcg ccggcgagcg cgccgcgtcc ggcggtcgcg gcggcgtgga ccgcgccggc
     1381 gacatccggg ccgccgtcgc cgcgatccgc gcgcgcacct agaccggggg cggcgcagcg
     1441 atgagcaccg aggcgaccat cgcgatgccg ttcaccgtcg cgtgtagggc gacgcacgga
     1501 tacaggctcc ccgtccgcga acgcaggtag gccaggccga ggcccgtgac gaggacccac
     1561 gggaggtcga gcaccgcgcc gtgcgcgagc gcgaacgcga cagcgctccc cacgatcgcg
     1621 agcggcgtgc cccacgcttc gagcaggccg tatccgagtc cgcggcatag cgattcctcg
     1681 acgaccggca cgtacaccgc gatgacgacg aagttcgcga tgaacggcgc gaggcgcgtg
     1741 gggtcggagt actgcgggac cgcctgctca cgcacggcat gacccaccgc gagctcgagc
     1801 gccagcgacg tgagccaggt cgcgccgagc gcgagcaggc cgacgcgcag tgcttcgccc
     1861 cacgacgtcg gccggcgcag cgcgaacgcc tcgcgcgtcg gaaggccgcg cgcgacgagc
     1921 agcgtgagca ggaggaggat cgtgtcgagc accagcgtgc cgatcgcggt ggaccagcgg
     1981 aagagcacgt ccggcaccgg ctcctccgct tgcgacgtga aggtcgcagc gtacgacacg
     2041 acagcgacga gaccgacgac ggcggcccac gccacgagcc ggcgcgggtg gcgggtcagg
     2101 cggtcgccgc cgccgcgcgc agcggcgcga gcagctcgag cgcgcggtcc atctcctccg
     2161 gcgtgttgaa gtagtgcggc gagagccgga tgaggccagg gcgggaatcg gtgatgacgc
     2221 cggaggcgcg cagggcgtcc accgcgcgct gcggatcggg atgctcgagc gtgacgatcg
     2281 cggtgcgctc gcgcgcgtcc gtcgggcagc gcaccgtcca cccctgcgcg cgcgcaccat
     2341 cgacgagccg ctgcgagagc agcatatggc gctcacggac ggtcgcgatg ccgatctcgc
     2401 gcagcagctc gatgcccgcg cgcgcggcat agacagcggc gaccgcgggc gtgccgtatt
     2461 cgaaacggcg cgccccgcgc ccgggctcca gatgctcgat gtcgaaggcg aacggatccg
     2521 cgacgcccca ccacccgacg gcgctgggat gcgcggcatc cgcgacggcg cgccgcgcgt
     2581 acagcaacgc gatgcccggg ccgcccatga gccacttcag cgtgc
//
LOCUS       DASTZR010000112         5999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_6807, whole genome shotgun sequence.
ACCESSION   DASTZR010000112 DASTZR010000000
VERSION     DASTZR010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6807"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1368)
                     /gene="nrfD"
                     /locus_tag="VFV20_02540"
     CDS             complement(<1..1368)
                     /gene="nrfD"
                     /locus_tag="VFV20_02540"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEU5287256.1"
                     /translation="MEALNAHEVAGVTAEWRARLEQRGLAPLRRSGRRYWVWIGALIA
                     IVAWGVYAYATQVANGLIVTGMRDRISWGVYIASFVFFIGISHAGTLLSAILRATKAR
                     WQLSITRMAELITVVALMVGALFPFIDMGRPDRILNLVSYGRWQSPLMWDILAISTYL
                     TGSTLYLFLPLVPDFALARDRLGPAAPRLQRAFFTLAAIGYHGLPEQRRALERAMTVM
                     MIVIIPVAVSVHTVVSWIFAMTLRVPMNTTVFGPFFVAGAIYSGIAAIVILMAVLRRV
                     LRLEEFITITQFRALGYLLAAFTLIMAYFNLQEYAVHGYKLEAGMPFHFEQLMTGPFA
                     ALFWFYLLGGIVLPGLLILHPRTRTIRGIVAASALVLLAMWIERYLIVVAGLRVPLMP
                     YTPADYLPSWVEWSILGGAFALFALIISIFAKIFPVISIWEVIEHRGPEPGAPPHTTP
                     VLPPRP"
     gene            complement(1356..2231)
                     /locus_tag="VFV20_02545"
     CDS             complement(1356..2231)
                     /locus_tag="VFV20_02545"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU5287257.1"
                     /translation="MRLSRRGFLARLGIGAGTAAMTAGLAPIAFGSPAAPAPAAAPRS
                     GRLRRWSMVIDLRKCDGCQSVAKAPQCTAACIQGHFAPEPMEWIQVYEAPLGERGTQF
                     IPTPCQQCQNAPCVNVCPVGATFATPEGVVLIDQQRCIGCRICMEACPFDRRFFNWGD
                     PPQPPETLFMEYSPEHQDPAIRGTVMKCDFCPDMARAGRLPYCAQGCPNRAIYYGDLE
                     EDVATNGVDVVSLSRFISENTGYRLKEELGTEPRVHYIPGHGEDVGRRASTRGRLPTT
                     WPWTKLLPGAKTWKR"
     gene            complement(2246..2863)
                     /locus_tag="VFV20_02550"
     CDS             complement(2246..2863)
                     /locus_tag="VFV20_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287258.1"
                     /translation="MKSDLRARALAILAGSAVLVSACGTAPAATAPTAAAPTAAAAVP
                     TAAPAATPQSLVVAHKLGETHKSQPKRLESVIEIDMGDHYFGNPQGAKNPTFTLPVGK
                     TVGIHLHNEGTVMHEIVVGRTAKKDGDYETTLSELIDFDVFFYYGDVKAEIGGAKFGE
                     IEVEQGIRDVWLRFTVPAELKGEWELGCFAPEHYAKGMHAKLIFQ"
     gene            complement(2977..3639)
                     /locus_tag="VFV20_02555"
     CDS             complement(2977..3639)
                     /locus_tag="VFV20_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004980055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5287259.1"
                     /translation="MTSAATKIRILVADDHTVIRRGIVGLLNAQADMEVIEEAGNGRE
                     AVARTETAKPDVVLLDVAMPELNGLGATRMIKDQSPDVQVLILTMHDREDYLFQALRA
                     GASGYVLKGADTDDLLTAVRSVAHGHVYLFPPVAKDLLNDYLRRVRSGEDEASYDGLT
                     EREREVLQLIAEGKTAGQIAEDLHISTHTVQSHRDSIMAKLGLHNRAALIRYALRRGL
                     VE"
     gene            complement(3636..5489)
                     /locus_tag="VFV20_02560"
     CDS             complement(3636..5489)
                     /locus_tag="VFV20_02560"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU5287260.1"
                     /translation="MGRFNRIGIQTRIMVYVTVGLAVMFGGFAYLGFRAVGDATELVY
                     DERLSTAYTTAATVERDFAHVAKDIDEALAPIDLRTPGEPAAASLRVLTHLAATDPFT
                     FFRITGIWILTAAGDAVTVGEPAIVAREQILMVRSAANELRGAPYHVIPAVGRPSTGT
                     AFGTIVVPVGGSASLPSMLVAIHATSVNSLADYTPLAASRADASTPVAQRGPGTYHLE
                     VLDPYGMAVLGIGADERPGEVSRHMPVVGDIMAKRDAAVIMHEPARGQSFPAHAMAVV
                     PLGASRFYLILEQPRDVALSLPIDLGRSVGFASVLGFLAALLVAWVTTRHVTRPTEEL
                     TVAAQRMARGDLESPIRVRAQDEVGRLAENLDAMRRQLRDAYRQISEANERLEGQVAE
                     RTARLSVVLGKVISAQEEERRRLARELHDETAQTMGALAIALDRARHGMGDASPDAIA
                     QLQEAQTIVKRLLEDTRRLILDLRPMALEDLGLVPAIRWYAESHLQDKGVGTTIELDR
                     PAVRLAPHLEVALFRIAQEAINNIARHADAASATIHLDFSDAAVRIAVADDGHGFDVD
                     RALASSDSVGLIGLQERARLLSGRMDINSQAGRGTQVTVELPIVLAAAQAP"
     gene            complement(5546..>5999)
                     /locus_tag="VFV20_02565"
     CDS             complement(5546..>5999)
                     /locus_tag="VFV20_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010474097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HEU5287261.1"
                     /translation="ERDEVVVEGTEDVVLAPHTVWHEYQFKGKPGDVRRRPPQCAPYH
                     LRLDWLMWFAAMSSPVYHPWFVPFVAKLLEADPATLALLRDDPFDGRRPRFVRARLYR
                     YRYTTPDERRESGAWWHRELQDEYLRPVSFASGTFQRGRDATSGAAGP"
BASE COUNT          906 a         2010 c         2179 g          904 t
ORIGIN      
        1 cggacgcggc gggagcaccg gggtggtgtg gggcggtgcg cccggctctg ggccgcggtg
       61 ctcgatgacc tcccagatcg agatgacggg gaagatcttc gcgaagatcg agatgatgag
      121 cgcgaagagc gcgaacgcac ccccgaggat cgaccactcg acccagctcg gcaggtagtc
      181 cgccggcgtg tacggcatca gcggaacgcg cagccccgcg acgacgatca gatagcgctc
      241 gatccacatc gcgaggagca cgagcgcgga tgcggcgacg atccctctga tggtccgcgt
      301 gcgcgggtgg aggatgagca gacctgggag cacgatgccg ccgaggaggt agaaccagaa
      361 gagcgcggcg aagggcccgg tcatgagctg ctcgaagtgg aagggcatgc cggcctcgag
      421 cttgtacccg tggaccgcgt attcctgcag gttgaagtac gccatgatca acgtgaaggc
      481 ggccaggagg tagccgagcg cgcggaactg cgtgatcgtg atgaactcct cgagacgcag
      541 cacccgccgc aggaccgcca tgaggatgac gatcgcggcg atgcccgagt agatcgcgcc
      601 ggcgacgaag aacggcccga acaccgtcgt gttcatcggc acgcgcagcg tcatcgcgaa
      661 gatccacgac accacggtgt gcaccgacac cgcgaccggg atgatcacga tcatcatcac
      721 cgtcatcgcg cgctcgagcg cgcggcgctg ctcgggaagg ccgtggtagc cgatcgccgc
      781 gagcgtgaag aacgcgcgct gcagccgcgg cgccgcgggg cccagccggt cgcgcgcgag
      841 cgcgaagtcg ggcacgagcg gaaggaagag gtacagcgtc gagccggtca gatacgtgct
      901 gatcgcgagg atgtcccaca tgagcggcga ctgccagcgg ccgtaggaca ccaggttgag
      961 gatgcggtcg ggacggccca tgtcgatgaa tgggaagagc gcgccgacca tgagggccac
     1021 gaccgtgatg agctcggcca tgcgcgtgat cgagagctgc cagcgcgcct tcgtcgcgcg
     1081 caggatcgcg gagagcagcg ttccggcgtg gctgatgccg atgaagaaga cgaagctcgc
     1141 gatgtacacg ccccacgaga tgcggtcgcg catccccgtg acgatgagcc cgttcgcgac
     1201 ctgcgtggcg tacgcgtaga cgccccaggc gacgatcgcg atgagcgcgc cgatccagac
     1261 ccagtaccgg cgtccactgc gtcgcagcgg ggcgagccca cgctgctcga gccgagcgcg
     1321 ccactcggcc gtgacgccgg cgacctcgtg cgcgttcaac gcttccacgt cttcgctccg
     1381 ggcagcagct tcgtccacgg ccacgtcgtc gggagacgac ccctggtgct cgcgcgccgg
     1441 ccgacgtcct cgccgtgtcc cgggatgtag tggacgcgcg gctcggtgcc gagctcctcc
     1501 ttgagccggt agccggtgtt ctccgagatg aagcgggaga gcgagacgac gtcgacgccg
     1561 ttcgtcgcga cgtcctcctc gaggtccccg tagtagatcg cgcgattcgg gcagccctgt
     1621 gcgcagtagg gcaggcgtcc cgcgcgcgcc atgtccgggc agaagtcgca cttcatcacc
     1681 gtcccgcgga tcgcgggatc ctggtgctcg ggcgagtact ccatgaacag ggtctccggc
     1741 ggctgcggcg ggtcgcccca gttgaagaac cgccggtcga atggacaggc ttccatgcag
     1801 atgcgacagc cgatgcagcg ctgctggtcg atgaggacca cgccctcggg tgtcgcgaac
     1861 gtcgcgccga cgggacagac gttcacgcat ggtgcgttct ggcactgctg gcacggggtc
     1921 ggaatgaact gcgtgccgcg ctcgccgagc ggtgcctcgt agacctggat ccactccatg
     1981 ggctccggtg cgaagtgccc ctggatgcac gcggcggtgc actggggtgc cttcgccact
     2041 gactggcacc cgtcgcactt tcgcaggtcg atgaccatcg accagcgacg gagccttccg
     2101 gaccgcgggg ccgcggcggg cgccggtgcg gcgggggagc cgaacgcgat cggcgccagg
     2161 ccggcggtca tcgccgccgt gcccgcgccg atcccgagtc gtgcgaggaa acctctgcgc
     2221 gacagtcgca ttgccgtccg ctccgctact ggaagatcag cttcgcgtgc atgcccttcg
     2281 cgtagtgctc cggcgcgaag cagccgagct cccactcgcc cttcagctcc gcggggaccg
     2341 tgaatcggag ccagacgtcg cggatgccct gctcgacctc gatctcgccg aacttcgcgc
     2401 cgccgatctc ggccttgacg tcgccgtagt agaagaagac gtcgaagtcg atgagctcgg
     2461 agagcgtcgt ctcgtagtcg ccgtccttct tggccgtgcg gccgacgacg atctcgtgca
     2521 tgaccgtgcc ctcgttgtgc aggtggatgc cgacggtctt cccgaccggc agggtgaagg
     2581 tcgggttctt cgcgccctgc gggttgccga agtagtgatc gcccatgtcg atctcgatga
     2641 cggattcgag acgcttcggc tggctcttgt gcgtctcgcc gagcttgtgc gccacgacga
     2701 gggactgtgg cgtcgcggcc ggcgccgcgg tcgggactgc ggccgccgcg gtcggcgctg
     2761 ccgcggtcgg tgcggtcgcc gccggtgcgg tgccgcaggc cgacacgagg acggcgctgc
     2821 ccgcaaggat cgcgagggct cgcgctcgca ggtcgctctt catggtcaaa cctcacacac
     2881 gcgcatcttt tcggggcgga cccctggggt gagcgtgcgc cgagcggtcc gcgtgcggca
     2941 ccgggtgttc gcgggatttc gggggcggag aacgccctat tcgacgaggc cccgccggag
     3001 cgcgtagcgg atcagcgcgg cgcggttatg gaggccgagc ttggccatga tgctgtcgcg
     3061 gtgggactgg acggtgtgcg tgctgatatg gaggtcctcc gcgatctgac cggccgtctt
     3121 gccctcggcg atcagctgca ggacctcacg ctcgcgctcg gtcaggccgt cgtagctcgc
     3181 ctcgtcctcc ccgctgcgca cgcggcgcag gtagtcattg aggaggtcct tcgccaccgg
     3241 ggggaagaga tagacgtgtc cgtgcgcgac ggatcgcacc gccgtcaaga ggtcgtcggt
     3301 gtctgcgccc ttcaggacgt agccggacgc gccggcgcgg agcgcctgga acaggtagtc
     3361 ctcgcggtcg tgcatcgtga ggatgaggac ctgcacgtcg ggcgactggt ccttgatcat
     3421 ccgcgttgcg ccgagcccgt tgagctcggg catcgcgacg tcgagcagca ccacgtccgg
     3481 cttggcggtc tccgtacggg cgacggcctc gcgaccgttc cctgcctcct cgatcacctc
     3541 catgtcggcc tgcgcgttga ggagcccgac gatgccgcgg cggatcacgg tgtggtcgtc
     3601 cgcgaccagg atgcggatct tcgtcgcggc gctcgtcatg gcgcctgcgc cgccgcgagc
     3661 acgatcggca gctcgacggt gacctgcgtg ccacgtcccg cctgcgagtt gatgtccatg
     3721 cggccgctga ggagtcgcgc gcgctcctgg agcccgatga ggccgacgct gtcggacgag
     3781 gcgagcgcgc gatccacgtc gaagccgtgg ccgtcatcgg cgaccgcgat ccggaccgcg
     3841 gcatcggaga agtcgaggtg gatggtcgcg ctggcggcat ccgcgtggcg cgcgatgttg
     3901 ttgatcgcct cctgcgcgat gcggaagagc gcgacctcga ggtggggcgc gagacggacc
     3961 gccggccggt cgagctcgat cgtcgtgccg acgcccttgt cctgcaggtg gctctccgca
     4021 taccagcgga tcgccgggac gaggccgagg tcctcgagcg ccatcgggcg gaggtcgagg
     4081 atgagccggc gcgtgtcctc gaggagccgc ttcacgatgg tctgcgcctc ctgcagctgc
     4141 gcgatcgcgt ccggcgatgc gtcgcccatc ccatggcgcg cgcggtcgag ggcgatggcg
     4201 agggcgccca tcgtctgcgc cgtctcgtca tggagctcac gcgcgagccg ccgccgctcc
     4261 tcctcctgcg ccgagatgac cttgccgagc acgacgctga ggcgcgcggt gcgctcggcc
     4321 acctggccct cgagacgctc gttcgcctcg ctgatctggc ggtaggcatc gcgcagctgc
     4381 cgccgcatcg cgtcgaggtt ctctgcgagc cggccgacct catcctgagc gcgcactcgg
     4441 atcgggctct cgagatcgcc gcgcgccatg cgctgcgcgg cgaccgtgag ctcctcggtc
     4501 ggccgcgtca cgtggcgcgt ggtgacccag gcgacgagca gcgccgcgag gaagccgagc
     4561 accgacgcga agccgacgga gcgtccgagg tcgatgggga gggagagcgc gacgtccctg
     4621 ggctgctcga ggatgaggta gaagcgcgac gcgccgagcg ggacgaccgc catggcgtgc
     4681 gccgggaagc tctggccgcg cgccggctcg tgcatgatca cggcggcatc gcgcttggcc
     4741 atgatgtcgc cgaccacagg catgtgccgg ctcacctctc cgggccgctc gtcggcaccg
     4801 atgccaagca cggccatccc gtacggatcg aggacctcga ggtggtacgt ccccggtccg
     4861 cgctgcgcga ccggcgtgct cgcgtcggca cgggaggccg ccagcggggt gtagtcggcg
     4921 aggctgttca ccgaggtcgc gtgtatcgcg acgagcatcg acgggagcga tgcgctgccg
     4981 ccgaccggca cgacgatcgt gccgaaggcc gtgcccgtcg acggccggcc gaccgcgggg
     5041 atcacgtggt agggcgcgcc gcgaagctcg ttggcggcgg accgcaccat gaggatctgc
     5101 tcgcgcgcca cgatcgcggg ctcgcccacc gtcacggcgt cgcccgcggc ggtgaggatc
     5161 cagatgccgg tgatgcggaa gaacgtgaag gggtcggtcg cggcgaggtg cgtcaggacg
     5221 cgcagcgatg ccgccgcggg ctcccccggc gtgcgcagat cgatgggcgc gagcgcctcg
     5281 tcgatgtcct tcgcgacgtg agcgaagtcg cgctcgaccg tcgccgcggt ggtgtaggcg
     5341 gtgctgaggc gctcgtcgta caccagctcg gtggcgtccc cgaccgcgcg gaatcccagg
     5401 tacgcgaagc ccccgaacat cacggccagg ccgacggtga cgtagaccat gatccgggtc
     5461 tggatgccga tgcggttgaa gcggcccatt cctcgttctc agccggctca cagcctcggc
     5521 ggtccgtgca cggcgtacta gaggcttacg gccctgccgc cccagacgtc gcgtcgcggc
     5581 cccgctgaaa cgttccggac gcgaacgaga ccggccggag gtactcgtcc tggagctcgc
     5641 gatgccacca cgcgccgctc tcgcgtcgct cgtcgggcgt ggtgtaccgg tagcggtaca
     5701 gccgcgcacg gacgaaacgc gggcgacgac cgtcgaatgg gtcgtcacgg aggagcgcga
     5761 gcgtcgccgg atccgcctcg agcagcttcg cgacgaacgg gacgaaccac gggtggtaca
     5821 cgggtgacga catcgccgcg aaccacatga gccagtccag gcgcaggtga tatggcgcgc
     5881 actgcggcgg ccggcgccgc acgtcaccgg gcttgccctt gaactggtac tcgtgccaca
     5941 cggtgtgcgg agcgagcacc acgtcctcgg tcccctcgac gaccacctcg tcgcgctct
//
LOCUS       DASTZR010000113         5469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_268044, whole genome shotgun sequence.
ACCESSION   DASTZR010000113 DASTZR010000000
VERSION     DASTZR010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5469
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268044"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VFV20_02570"
     CDS             <1..306
                     /locus_tag="VFV20_02570"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU5287262.1"
                     /translation="LDRKFSVLIYPEGKLTIGGPTQPFKTGTGLIAVEGGTPVVPMKL
                     KIGNVSILDHWDRTWEPFRSNGWRGRVEVVFGDALYFPAGTSPADATAKLQAAVAAL"
     gene            303..1385
                     /locus_tag="VFV20_02575"
     CDS             303..1385
                     /locus_tag="VFV20_02575"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU5287263.1"
                     /translation="MSASDGQDPPIDRWWVTRVAIAAAAVVLVLTAIRLILDVAALLS
                     HVIVLVLFGVVVAFTLGPLVEMLQRRLGRRSLAVLVTAVTAISVLVIAIALLAVPLVR
                     ETRALADDVPRYAALLASDEPMQVLGVEISGELRERVGAEIGARVGDWSQQAARVALR
                     IGSGIVDVLFVLVLGIYLLASAPRTRAWLFARAPRSRRAEIERVEAEARRLFGAYIRG
                     QMLLGLIIGTVSGVAYAALGVPYAVFLGVLAGLLELVPIVGPVVAGTIAAAVALTQPW
                     PLVLWVIVAAILIQQLENNLLVPRISGTAVGLHPLAALLAVLVGAELGGIIGAIFAVP
                     VTGLVWSVWRARESEASSPPPSSPRS"
     gene            complement(1306..2244)
                     /locus_tag="VFV20_02580"
     CDS             complement(1306..2244)
                     /locus_tag="VFV20_02580"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HEU5287264.1"
                     /translation="MSLRATPIIVSSGNGANALADGMRILRLGGSALDAVEACARYVE
                     ADVTDTSVGRGGLPNIIGQVELDASLVDGTTHRVGAVAGLRGFLHPVSVARAVMERLP
                     HVMLVGDGAARFAAEIGAERAENLTASTRKLWVERLAKVRETPASVRARAKLVPVVLR
                     TVGENRGTVNFIALDRKGHVASAVSTSGWAYKYPGRVGDSPIVGAGNYCDTKFGGATC
                     TGFGELAIRNVTAKTAVDRLALGLGPTLVARRAIEDANVLDDAAFNIVVLAADGTHAS
                     ATNRAGRQYAFMTAAMTEAATKPRTLVRATRSRRGR"
     gene            complement(2241..2873)
                     /locus_tag="VFV20_02585"
     CDS             complement(2241..2873)
                     /locus_tag="VFV20_02585"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEU5287265.1"
                     /translation="MPATSPTGASAPEPDVVVIPAGDALLDDPPRTIHVNVFAIARRP
                     VTNEDFAAFVADASYTAAGWRTGLLDHPAEGISWADAVAYCRWLSVATARVYRLPDER
                     EWEKAARGSSGRRWPWGTEDEPARANVREAKVGGTSAIGKYPLGASPEGVVDLIGNVR
                     EWTNTWADGRVLRGGSYLDPLGDCLPHKRFRPGDDLRGHGFRVVRSMTGR"
     gene            2977..3198
                     /locus_tag="VFV20_02590"
     CDS             2977..3198
                     /locus_tag="VFV20_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287266.1"
                     /translation="MDLRPVVWDATVVTPRDDRGVTIVLRARYRTLEEICTDGGADTR
                     SLASFTPVRHGRVDHAVRADHAWTEAAFA"
     gene            complement(3195..4937)
                     /locus_tag="VFV20_02595"
     CDS             complement(3195..4937)
                     /locus_tag="VFV20_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEU5287267.1"
                     /translation="MAKRIKVVAKDPAPMPGRPWTKFYDEGVPATLTYPDTTMHAMLD
                     DAAESYPDATCTIFFNAKRSYRSISDDAWRFANALRAMGVKKGDRVAIMLPNTPQFIV
                     AFYGALRSGAIVVPCNPLYTVPELRHQLEDSGAETMVVLSRLYPVVKAAREGTKITRV
                     LVTNIKEEMPPILRTLFTIAKEKKDGHRQHFEGDPGARAFRQVVKDAPATAFDAGTTS
                     RDTALLQYTGGTTGVSKGAMLSHHALVANTLQCRAWFAHLEESKGVAMAVMPFFHVYG
                     LTVVMSLAVQSASAMVLEPQFELERVLKDVARHKPTTFSGAPRIYNAIINSPLAKQYD
                     MRSIQACISGSAPLLVETHRKFTELTGANLVEGYGLTEAAPVTHCNPLYGKGKQKIGS
                     IGIAYPDVECKIVDSETGQHEMAVGEPGELILRGPQLMDGYYNRPDETKTTIRDGWLY
                     TGDIATVDEDGYYAIVDRKKEMIIVSGFNVYPREVEEALATHPAVLDSAAIGKPHPIK
                     GEDVKAFVVLKPGTNATAEELIAHTRTQLAPFKVPHEIEFRESLPKTLIGKTLRRQLA
                     EEDRKKREKAGAPA"
     gene            complement(5013..>5469)
                     /locus_tag="VFV20_02600"
     CDS             complement(5013..>5469)
                     /locus_tag="VFV20_02600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5287268.1"
                     /translation="MLAAVRARAARSPLGARFEARGADAQALPFADASFDRVTAGFAV
                     RNFGDLDAGLREMRRVLLPTGRAVILELSTPPNPVVRACFRLYFHRIAPRLAALLGGD
                     AAAYRYLPRSVARFPGAVPLAERLRSAGFTRVRFERLALGIAAIHVAEP"
BASE COUNT          717 a         1910 c         1965 g          877 t
ORIGIN      
        1 ctggatcgga agttctcggt gctcatctat ccggaaggga agctgaccat cggcggtccg
       61 acgcagccct tcaagacggg cacgggcctc atcgcggtcg agggcgggac gccggtcgtc
      121 ccgatgaaac tgaagatcgg gaacgtctcg atcctggacc actgggaccg cacctgggag
      181 ccgttccggt cgaacggctg gcgcggccgc gtggaggtcg tcttcggcga cgcgctctac
      241 ttcccggccg gcacctcgcc cgcggatgcg acggcgaagc tccaggcggc ggtcgccgcg
      301 ctgtgagcgc gagcgacggc caggatccgc cgatcgaccg ctggtgggtg acgcgcgtcg
      361 cgatcgcagc cgcggcggtc gtgctcgtcc tcacggcgat ccgcctcatc ctcgatgtcg
      421 ccgcgctgct ctcgcacgtc atcgtgctcg tcctcttcgg cgtcgtcgtc gcgttcacgc
      481 tcggcccgct ggtcgagatg ctgcagcgac gcctcggacg acggtccctc gccgtcctcg
      541 tgaccgctgt gacagcgatc tcggtgctgg tgatcgcgat cgcgctcctc gcggtgccgc
      601 tcgtgcgtga gacccgggcg ctggcggatg acgtcccgcg ctacgcggcg ctgctcgcga
      661 gcgacgagcc gatgcaggtc ctcggcgtgg agatatcggg tgagctgcgc gagcgcgtcg
      721 gcgccgagat cggcgcgcgg gtcggcgact ggtcgcagca ggcggcacgc gtcgcgctgc
      781 gtatcgggag cgggatcgtc gatgtcctct tcgtgctcgt gctcggcatc tacctgctgg
      841 cgagtgcgcc gcggacacgg gcgtggctct tcgcgcgcgc accgcggtcg cgccgggccg
      901 agatcgagcg cgtcgaggcg gaggcacgcc gcctgttcgg cgcgtacatc cgcgggcaga
      961 tgctgctcgg gctcatcatc ggcacggtct cgggcgtcgc gtacgcggcg ctgggcgtgc
     1021 cctacgcggt tttcctcggc gtcctcgccg gcctgctgga gctcgtgccg atcgtcggtc
     1081 cggtcgtggc cgggacgatc gcggcggcgg tcgcgctgac gcagccgtgg ccgctcgtgc
     1141 tctgggtcat cgtcgccgcg atcctcatcc agcagctcga gaacaacctg ctcgtgccgc
     1201 gcatctcggg cacggccgtc ggcctccatc cgctggccgc gctgctcgct gtgctggtcg
     1261 gtgcggagct cggcggcatc atcggcgcga tcttcgcggt gccggtcacc ggcctcgtct
     1321 ggagcgtgtg gcgcgcacga gagtccgagg cttcgtcgcc gcctccgtca tcgccgcggt
     1381 catgaacgcg tactgacggc ccgcgcggtt cgtcgccgac gcgtgcgtgc cgtcggcggc
     1441 gagcacgacg atgttgaacg cggcgtcgtc gaggacgttc gcatcctcga tcgcgcgccg
     1501 cgccacgagc gtcgggccga gccccagcgc gagacggtcc acagcggtct tcgcggtgac
     1561 gttgcggatc gcgagctcgc cgaagccggt gcaggtcgcg ccaccgaact tcgtgtcgca
     1621 gtagttcccg gcgccgacga tcggcgaatc gccgacgcgc ccggggtact tgtaggccca
     1681 gccgctcgtg ctgaccgccg aagcgacgtg acccttccgg tcgagcgcga tgaagttcac
     1741 cgtgccacgg ttctcgccga ccgtccgcag cacgaccggc acgagcttcg cgcgcgcgcg
     1801 aacgctcgcg ggcgtctcgc gcaccttcgc gagccgctcg acccagagct ttcgcgtgga
     1861 cgcggtgagg ttctccgcgc gctcggcgcc gatctcagcg gcgaagcgtg cggcgccgtc
     1921 gccgacgagc atcacgtgcg gcagccgctc catgacggcg cgcgcgacgc tgaccggatg
     1981 gaggaagccg cgcagccccg cgaccgcgcc gacgcgatgc gtcgtgccgt cgacgagaga
     2041 tgcgtcgagc tcgacctggc cgatgatgtt cggcagtccg ccgcggccga cgctcgtgtc
     2101 cgtgacatcc gcctcgacgt accgcgcgca cgcctcgaca gcgtcgagcg cgctgccccc
     2161 gagtctcagg atccgcatgc cgtcggcgag cgcgttggcg ccgttcccgc tgctgacgat
     2221 gatgggcgtc gccctgaggc tcatcggccg gtcatggagc ggacgacgcg gaagccgtgg
     2281 ccgcgcaggt cgtcgccggg acggaagcgc ttgtgaggga ggcagtcgcc gagcgggtcg
     2341 aggtacgagc cgcccctgag cacgcggccg tcggcccagg tgttcgtcca ctcgcggacg
     2401 ttcccgatga ggtcgacgac gccctcgggg gacgcgccca gcgggtactt cccgatcgcg
     2461 gacgtaccgc cgaccttcgc ctcgcgcacg ttggcccgcg cgggctcgtc ctcggtgccc
     2521 cagggccagc ggcggcccga ggagccgcgg gcggccttct cccactcacg ctcgtcgggc
     2581 aggcggtaga cgcgtgcggt ggccacgctg agccagcggc agtaggcgac ggcgtccgcc
     2641 cacgagatgc cctcggccgg atggtccagc aggcccgtcc gccagcccgc ggccgtgtac
     2701 gaggcgtccg cgacgaacgc ggcgaagtcc tcgttcgtca ccggccgccg cgcgatcgcg
     2761 aagacgttga cgtggatcgt gcgcggcgga tcgtccagga gcgcgtcacc ggccggtatg
     2821 acgacgacgt ctggctcagg ggccgaggcc cctgtgggcg aagtggcggg cacggtcgga
     2881 gattaaccgc ggtggacggt cgtcgcccga acgggggatg agagtttcac ccgtgcggtg
     2941 gatggatggg atgcgagcgc gcggcaccat gccggcgtgg acctccgccc ggtggtgtgg
     3001 gacgcgacgg tagtgacgcc gcgggatgac cgcggcgtca ccatcgtcct gcgtgcgcgc
     3061 taccggaccc tcgaggagat ctgcaccgac ggcggcgcgg acacgcgctc gctcgcgtcg
     3121 ttcacgccgg tccgccacgg tcgcgtcgac cacgccgtcc gcgccgatca cgcctggacc
     3181 gaggccgcgt tcgcctaggc gggcgccccc gccttctcgc gcttcttccg atcctcttcc
     3241 gcaagctgcc tgcgcagcgt cttgccgatg agcgtcttcg gcagcgactc gcggaactcg
     3301 atctcgtgcg gcaccttgaa cggcgcgagc tgcgttcgcg tatgcgcgat gagctcctcc
     3361 gcggtggcat tcgtgcccgg cttgaggacg acgaaggcct tcacgtcctc gcccttgatc
     3421 ggatgcggct tcccgatcgc cgcgctgtcc aggaccgcgg gatgcgtcgc gagcgcctcc
     3481 tcgacctcac ggggatacac gttgaagccg gagacgatga tcatctcctt cttgcggtcg
     3541 acgatcgcgt agtagccatc ctcgtccacg gtcgcgatgt cgccggtgta caaccacccg
     3601 tcacggatcg tggtcttcgt ctcgtcggga cggttgtagt agccgtccat gagctgcggg
     3661 ccgcgcagga tgagctcgcc cggctcgccg acggccatct cgtgctggcc ggtctcggag
     3721 tcgacgatct tgcattccac gtcgggatag gcgatcccga tggagccgat cttctgcttc
     3781 cccttcccgt agagcgggtt gcagtgcgtc acgggcgcgg cctcggtgag gccgtagccc
     3841 tcgacgaggt tcgcgccggt gagctcggtg aacttccggt gcgtctcgac caggagcggc
     3901 gcagatcccg agatgcacgc ctggatcgat cgcatgtcgt actgcttcgc gaggggcgaa
     3961 ttgatgatcg cgttgtagat ccgtggcgcg ccgctgaacg tcgtgggctt gtgacgggcg
     4021 acgtccttca gcacgcgctc gagctcgaac tgcggctcga gcaccatcgc ggacgcgctc
     4081 tgcacggcga ggctcatcac gacggtgagg ccgtacacgt ggaagaacgg catgaccgcc
     4141 atggccacgc ccttgctctc ctcgaggtgc gcgaaccacg cgcggcactg cagggtgttc
     4201 gcgacgagcg cgtggtgcga cagcatcgcg cccttggaca cgccggtcgt gccgccggtg
     4261 tactggagca gcgcggtgtc ccgcgaggtc gtcccggcgt cgaacgccgt cgccggcgcg
     4321 tccttgacca cctggcggaa cgcgcgcgcc cccgggtcgc cctcgaagtg ctgccggtgg
     4381 ccgtccttct tctccttcgc gatggtgaag agcgttcgca ggatcggcgg catctcctcc
     4441 ttgatgtttg tgacgaggac gcgcgtgatc ttggtgccct cgcgcgccgc cttcaccacc
     4501 ggatagagac gcgacaggac gaccatcgtc tccgcgcccg agtcctcgag ctggtggcgc
     4561 agctcgggca cggtgtagag cgggttgcac gggacgacga tggcaccgct gcgcagcgcg
     4621 ccgtagaagg cgacgatgaa ctgcggcgtg ttcggcagca tgatcgcgac gcggtcgccc
     4681 tttttgacgc ccatcgctcg cagtgcgttc gcaaagcgcc acgcgtcgtc ggagatcgac
     4741 ctgtagctgc gcttcgcgtt gaagaagatc gtgcacgtgg cgtcggggta gctctccgcg
     4801 gcgtcgtcga gcatcgcgtg catcgtcgtg tccggatagg tgagcgtcgc gggcacgccc
     4861 tcgtcgtaga acttcgtcca cggtcggccg ggcatcggtg ccgggtcctt cgcgacgacc
     4921 ttgatgcgct tcgccatcgt cacggctccc ttcggccgcc ccacggcggc ctctcccgtc
     4981 ccggtcggtc cccgctggac tctactaccg gctcagggtt cggcgacgtg gatggccgcg
     5041 atgccgaggg cgagccgctc gaagcggacg cgcgtgaagc ccgcgctccg cagacgctca
     5101 gcgagcggca cggcgcctgg gaaccgcgcg accgaccgcg gcaagtaccg gtatgcggcg
     5161 gcatccccgc ccaggagcgc ggccaggcgt ggcgcgatcc ggtggaagta cagcctgaag
     5221 cacgcgcgga ccacaggatt cggcggcgtc gacagctcga ggatcaccgc gcgccccgtc
     5281 ggcagcagga cgcgccgcat ctcacggagg ccggcgtcga ggtcgccgaa gttgcggacg
     5341 gcgaagcccg cggtcacgcg gtcgaaggac gcgtcggcga agggcagcgc ctgagcgtcg
     5401 gcgccacgcg cctcgaagcg cgcgccgagc ggcgagcgcg ccgcacgcgc gcggaccgcc
     5461 gcgagcatg
//
LOCUS       DASTZR010000114         6732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_397203, whole genome shotgun sequence.
ACCESSION   DASTZR010000114 DASTZR010000000
VERSION     DASTZR010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6732
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397203"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1606
                     /locus_tag="VFV20_02605"
     CDS             <1..1606
                     /locus_tag="VFV20_02605"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HEU5287269.1"
                     /translation="TSEFVLPWVRLHASKDYLHMARLLQEFPRVKANFTMVPSLRDQL
                     EDYARGADDEDTRVTMRTVDGDLTHEEKQHMLRRFFSIHHGNVIHRHDEYRRLLQIAL
                     AAGDRAELLSDDYFVDLALWFNLAWIDPDDVASDARLKSLRENGRGFAREDVRYVIGR
                     QRLMASGVPHLYHRLEHDGQLELLTVPYYHPILPLLIDSKSALRADPDAIMPDPPFAY
                     PDDAAFHLEEAIASHERAFGKKPRGTWPSEGSVSPEAAAAIFKSGLEWFASDEGVLAA
                     SLGTHFQRDDIGALVEPALLYRPYRVAGGTAIFRDREMSDRIGFLYGDMSPTDAVGDM
                     IWKLERARDRLSDDGGPYLAAIILDGENAWEQYPNNGNDFLRQLYGTLQSDERFETVR
                     VSDYLDRSPAKVELPNLHTGSWIDANLRVWMGEPAHARAWSALERTRRFAQEQWGALR
                     YMPKSVRQPLMVAEGSDWFWWYSSRNSSPEDAVFDALFRANLEVVWWYAGAEPPDDIR
                     QPLMDPQRLPGEKGGAMLPGAREQHG"
     gene            1590..1940
                     /locus_tag="VFV20_02610"
     CDS             1590..1940
                     /locus_tag="VFV20_02610"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5287270.1"
                     /translation="MSSTASPAFGRVGRQVQAPSALRPAAEDEIGAWGGPRLFRGRRV
                     LDLGCGDGRLALGIAPYAREVHGIDPDAALIRSARARARARGARNARFAVGAAQELPY
                     DDRAFDVVVLSWTL"
     gene            1959..2381
                     /locus_tag="VFV20_02615"
     CDS             1959..2381
                     /locus_tag="VFV20_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287271.1"
                     /translation="MVDALGEMHRVLKPGGTLVDARPDSRVLGYAEHVTPRGFVRVGT
                     IRTAREELKNDRASDHAIATVVRRGLFRHGPRGRLWHRVPFEDLDALRQYLSEHLRFE
                     KRARWTIDAATRRRIRGDRFVIRRGVRYELLERLPGTR"
     gene            complement(2345..3142)
                     /locus_tag="VFV20_02620"
     CDS             complement(2345..3142)
                     /locus_tag="VFV20_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287272.1"
                     /translation="MSEDLARLFDFDPRPDPCESDEEIVHQRDRLHRYRPTATHSPWS
                     NGVHWVRWDEQAVERGIDDVLAYYAARDAGFIWTVGPDTTPRDLGERLERRGLHLEAR
                     TAMLVATLPIGGLRTNPELVVRAVDDEARMRDSISVEHPEWDEERTSALLRERMAYLA
                     CPGHSRHAVIAYLGGVPISAARWRVSTLHPSIHLAGAETLAAYRGRGAYSTLVDYRTR
                     IGLTIGCRYATIFADEATSAPILAKRGFRIVGRCAHYLVPGSRSSSS"
     gene            complement(3139..4386)
                     /locus_tag="VFV20_02625"
     CDS             complement(3139..4386)
                     /locus_tag="VFV20_02625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5287273.1"
                     /translation="MWNVVLVAFEGPDRYAFVGGLATRMNDLAEALVARGHMVRHLFV
                     GDPSLPHLEERQDGRLVLERWSQWISSYHPKDVYDGEDGKYLDFSRTVPPRLAEVVAE
                     NDARGERTVLLFEDWQTAAAAIGTATLLASRDLRAPVFWNANNTYGFGRVDFPLLQRL
                     VSITTISRYMRMELAKVDVEAAILPNGIADRWLKPIGPSDVAALRRSFGDRPTFAKVA
                     RFDRDKRWLWALDAVAAMRDAGAQPRFIIRGSRSEYADIVWARVRDLGLDLDRVVLPP
                     TATPKDLAGAIAATTGNVIFLDFFIPERTLRALYGAVDGVLANSEKEPFGLVGLEVMA
                     CGGIAYVGRTGEDYAVPLGNAVVVQSEDPRELLTSYDTLRARPELVSAMRADGRATAK
                     RFAWPRILEGYEVLWETALALTS"
     gene            4409..5677
                     /gene="serS"
                     /locus_tag="VFV20_02630"
     CDS             4409..5677
                     /gene="serS"
                     /locus_tag="VFV20_02630"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEU5287274.1"
                     /translation="MPVLQRVRDDPERVRAMLTARHFEAPLDKLIELDARVRQLQSEK
                     EQLQAERNKASKGGPPSDAVKQRMREVGERIKQIDAELGPLSAERDATASWIPNDVAP
                     DVPPGKDEHDNPVLREDAKRELGFPAKPHWEVGEALGILDIPRGVKLSGSRFYVLRGA
                     GAALQRALIAWMIDLKLEAGLGFTEIYPPFITRKETLLASAHLPHFDENLYRDDDDDV
                     WLIPTAETQLISLHRDETIPVERLPIRYVAYTACFRREKMSAGRDVRGIKRGHQFDKV
                     EMVVLTRPEDSRAELDLLTELAVQVLEKLELPARVVERCAGDLGFVAMKGFDVETWAP
                     GVQEWLEVSSTSDGGSLQAERAAIRFKREAGGKVEHPHLLNASGLALPRLMISVIENY
                     QQQDGSLVIPKPLRQYMRGRERVEPGEFSL"
     gene            complement(5685..6203)
                     /locus_tag="VFV20_02635"
     CDS             complement(5685..6203)
                     /locus_tag="VFV20_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287275.1"
                     /translation="MNTTPTPQRQWLAGLALIGIGLVLLAIELFQPKDADWIVLGSIS
                     ILLFAFYAGTRQDGFLVPGAILGGLAIGVGLEDFGYSMNGGAVVLGLGAGFLAIYVID
                     NLFGAHPHWWPLIPGGILAVVGGSQAIGGTAAADAVARWWPAVLIVVGLFVLLAGQRQ
                     RNTPAPESRAKS"
     gene            complement(6220..>6732)
                     /locus_tag="VFV20_02640"
     CDS             complement(6220..>6732)
                     /locus_tag="VFV20_02640"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEU5287276.1"
                     /translation="SAGTATQPDVVGYIERVPRIAGLWLDREAGLLRWAPLLALGYGL
                     YRTAGEHRERSAAGPRGFERPPVKLLTDGPYAYTRNPMYLGHLVFCTALFGATRSPIA
                     LLLALRQARRLAERVRTDEVRLEQIFGDEYRAYVARVPRWIGLPRVSSVHGTPVATNG
                     TWQRPEREGA"
BASE COUNT         1023 a         2386 c         2390 g          933 t
ORIGIN      
        1 gacgagcgag ttcgtgctgc cgtgggtgcg tctgcacgcg tcgaaggact acctccacat
       61 ggcgcggctc ctccaggagt tcccgcgcgt gaaggcgaac ttcacgatgg ttccgagcct
      121 gcgcgaccag ctcgaggact acgcgcgcgg ggccgacgac gaggacacgc gcgtcacgat
      181 gcgcaccgtc gacggcgacc tcacacacga ggagaagcag cacatgctgc gccgcttctt
      241 ctccatccac cacggcaacg tcatccaccg tcacgacgag taccgccggc tgctgcagat
      301 cgcgctcgcc gcaggcgacc gcgccgagct cctctcggac gactacttcg tcgatctcgc
      361 gctttggttc aacctcgcgt ggatcgaccc cgacgacgtc gcgagcgacg cgcggctgaa
      421 gtcgctgcgc gagaacggcc gcgggttcgc gcgcgaggac gtgcgctacg tgatcggccg
      481 gcagcggctc atggcgtcgg gcgtcccgca cctctatcac cgtctcgagc atgacggcca
      541 gctcgagctg ctcaccgtgc cctactacca cccgatcctg ccgctgctca tcgacagcaa
      601 gagcgcgctg cgcgcggacc cggacgcgat catgcccgat ccaccattcg cgtaccccga
      661 cgacgccgcc ttccatctgg aggaggcgat cgcgtcgcat gagcgcgcct tcggcaagaa
      721 gccgcgcggc acctggccgt cggaggggtc ggtctcgccc gaggcggcgg ccgcgatctt
      781 caagagcggc ctcgagtggt tcgcctccga cgagggcgtg ctcgccgcgt cgctgggcac
      841 ccatttccag cgcgatgaca tcggcgccct cgtggagccc gcgctcctct accggccgta
      901 ccgcgtcgcg ggcggcaccg cgatcttccg ggaccgcgag atgtccgacc gcatcggctt
      961 cctctacggc gacatgagcc ccaccgacgc ggtcggcgac atgatctgga agctggagcg
     1021 ggcgcgcgac cggctctccg acgacggcgg tccctatctc gcggcgatca tcctcgacgg
     1081 cgagaacgcc tgggagcagt acccgaacaa cgggaacgac ttcctgcgcc agctgtacgg
     1141 cacgctgcag tccgacgagc gcttcgagac ggtgcgcgtc tccgactacc tcgatcgcag
     1201 ccccgcgaag gtcgagctcc cgaacctgca cacggggtcg tggatcgacg ccaacctgcg
     1261 cgtgtggatg ggggagcccg cgcacgcgcg tgcgtggagc gcgctcgagc gcacgcggcg
     1321 cttcgcgcag gagcagtggg gcgcgctccg ctacatgccc aagagcgtgc gccagccact
     1381 catggtcgcc gagggcagcg actggttctg gtggtactcg agccgcaact cgagcccgga
     1441 ggacgcggtg ttcgacgcgc tcttccgcgc gaacctcgag gtcgtgtggt ggtacgcggg
     1501 cgccgagccg cccgacgaca tccgccagcc cctcatggat ccgcagcggc tgcccggcga
     1561 aaagggtggc gctatgctgc ccggcgctcg tgagcagcac ggctagcccc gccttcggtc
     1621 gggtcggacg gcaggtccaa gcgccgtccg ccctgcgccc cgccgccgag gacgagatcg
     1681 gcgcctgggg tggcccacgc ctcttccgcg gtcgtcgcgt gctcgatctc ggatgcggtg
     1741 acggtcgcct ggcgctcggc atcgctccct acgcgcgcga ggtgcatggc atcgaccccg
     1801 acgctgcgct catccggagc gcgagggcgc gcgcgcgtgc ccgcggcgcg cggaacgcgc
     1861 gtttcgccgt cggcgcggcg caggagctgc cgtacgacga ccgcgcgttc gacgtggtgg
     1921 tcctctcgtg gacgctctga tgtatcgcac ggcggggcat ggtcgatgcc ctgggagaga
     1981 tgcaccgggt gctgaagcct ggcggcacgc tggtcgacgc gcggcctgac tcgcgcgtcc
     2041 tcgggtacgc cgagcacgtg accccgcgcg gcttcgtgcg ggtgggcacg atccgcaccg
     2101 cgcgcgagga gctgaagaac gaccgcgcct ccgaccacgc catcgcgacc gtcgtgcgac
     2161 ggggtctctt ccgtcacggc ccgcgcggtc gcctgtggca ccgcgtcccg ttcgaggacc
     2221 tcgacgcgct gcgccagtac ctctcggagc accttcgctt cgagaagcgc gcaaggtgga
     2281 cgatcgacgc ggcgacgcgt cgccgcatcc gcggcgatcg gttcgtgatc cgccgcgggg
     2341 tgcgctacga gctgctggag cggctgcccg gcacgaggta gtgcgcgcac cgcccgacga
     2401 tccggaagcc gcgcttggcg aggatcggcg ccgacgtcgc ctcgtccgcg aagatcgtcg
     2461 cgtaccggca gccgatcgtg agcccgatcc tggtgcggta gtccacgagc gtggagtacg
     2521 cgccgcggcc gcggtacgcg gccagggtct ccgcgcccgc caggtggatg gacggatgga
     2581 gcgtgctcac gcgccagcgc gcggcggaga tcggcacgcc tccgaggtac gcgatgaccg
     2641 cgtggcgcga atgcccagga cacgccaggt aggccatgcg ctcgcgcagc agtgccgacg
     2701 tgcgctcctc gtcccactcc ggatgctcga ccgagatcga gtcgcgcatc ctcgcctcgt
     2761 cgtccacggc gcgcacgacg agctccgggt tcgtccgcag cccgccgatg ggcagcgtcg
     2821 cgacgagcat cgcggtgcgc gcctcgaggt ggaggccgcg gcgctcgagg cgctccccga
     2881 gatcgcgcgg cgtggtgtcg ggcccgaccg tccagatgaa tccagcgtcg cgcgccgcgt
     2941 agtaggcgag gacgtcgtcg atcccgcgtt cgacggcctg ttcgtcccag cgcacccagt
     3001 gcacgccgtt cgaccacggg ctgtgcgtcg cggtgggccg gtagcggtgg agccgatcgc
     3061 gctggtggac gatctcctcg tcggactcgc agggatcggg ccgcgggtcg aagtcgaaga
     3121 ggcgcgcgag gtcctcgctc acgaggtgag cgcgagcgcg gtctcccaga ggacctcgta
     3181 gccctcgagg atccggggcc acgcgaagcg cttggcggtc gcccggccgt cggcgcgcat
     3241 ggccgacacg agctccggcc gcgcgcgcag cgtgtcgtac gacgtcagca gctcgcgggg
     3301 gtcctcgctc tgcacgacga cggcgttgcc gagcggcacc gcgtagtcct cgcctgtacg
     3361 tccgacgtag gcgatgccgc cgcacgccat gacctccagg ccgacgaggc cgaacggctc
     3421 cttctccgag ttcgcgagca cgccgtcgac cgctccgtac agcgcgcgca gcgtgcgctc
     3481 ggggatgaag aagtcgagga agatcacgtt gccggtcgtc gccgcgatcg cgccggcgag
     3541 atccttgggt gtggcggtcg gcggcaggac cacgcggtcc agatcgagcc cgaggtcgcg
     3601 gacgcgtgcc cagacgatgt ccgcgtactc gctgcgactc ccgcggatga tgaagcgcgg
     3661 ctgcgcaccg gcatcgcgca tcgccgcgac ggcgtcgagc gcccagagcc accgcttgtc
     3721 gcggtcgaag cgcgcgacct tcgcgaacgt cggccggtcc ccgaacgatc ggcgcagcgc
     3781 cgcgacgtcg gagggcccga tcggcttcag ccagcggtcg gcgatgccgt tgggcaggat
     3841 cgcggcctcg acgtcgacct tcgcgagctc catgcgcatg tagcgggaga tcgtcgtgat
     3901 cgacacgaga cgctggagca gcgggaagtc gacgcggccg aagccgtacg tgttgttcgc
     3961 gttccagaac accggcgcgc ggagatcccg cgacgcgagg agcgtcgccg tgccgatcgc
     4021 ggcggccgcg gtctgccagt cctcgaacag gaggaccgtg cgctcgccac gcgcgtcgtt
     4081 ctcggcgacg acctccgcga ggcgtggcgg cacggtgcgc gagaaatcga ggtatttgcc
     4141 gtcctcgccg tcgtacacgt ccttcgggtg gtacgagctg atccactgcg accaccgctc
     4201 cagcacgagc cggccgtcct ggcgctcctc gaggtgcggc aggctcgggt cgccgacgaa
     4261 gaggtggcgc accatgtggc cccgggccac gagcgcctcc gcgaggtcgt tcatgcgcgt
     4321 ggcgaggccg ccgacgaacg cgtagcggtc gggaccctcg aaggcgacga ggacgacgtt
     4381 ccacatgagt gccgataatg gcacgaccgt gcccgtcctc cagcgcgtcc gcgacgatcc
     4441 cgagcgcgtc cgcgcgatgt tgaccgcgcg ccacttcgag gcgccgctcg acaagctgat
     4501 cgagctggac gcgagggtgc gccagctgca gagcgagaag gagcagctcc aggccgaacg
     4561 caacaaggcc agcaaggggg ggccgccatc ggatgcggtg aagcagcgca tgcgcgaggt
     4621 gggagagcgc atcaagcaga tcgacgccga gctcggcccg ctctccgccg agcgggatgc
     4681 gacggcgtcg tggatcccga atgacgtcgc gcccgacgtc ccgccgggaa aggacgagca
     4741 cgacaatccc gtgctccgcg aggacgccaa gcgcgagctc ggcttcccgg cgaagcccca
     4801 ctgggaggtc ggcgaggcgc tcggcatcct cgacatccca cgcggcgtga agctgtccgg
     4861 gtcgcgcttc tacgtgctgc gcggtgcggg cgcggcgctc cagcgtgcgc tcatcgcgtg
     4921 gatgatcgac ctcaaactcg aggccgggct cggcttcacc gagatctacc cgccgttcat
     4981 cacccgcaag gagacgctgc tcgcgtcggc gcatctcccg cacttcgacg agaacctgta
     5041 tcgcgacgac gacgacgacg tctggctcat cccgaccgcg gagacccagc tcatctcact
     5101 gcaccgcgac gagacgatcc ccgtcgagcg cctgccgatc cgctacgtcg cgtacaccgc
     5161 gtgcttccgg cgcgagaaga tgagcgcggg acgcgacgtc cgcggcatca agcgcggcca
     5221 ccagttcgac aaggtcgaga tggtcgtgct caccaggccg gaggactcgc gcgctgagct
     5281 cgatctgctc accgagctcg ccgtccaggt gctcgagaag ctcgagctcc ccgcgcgcgt
     5341 cgtcgagcga tgcgcgggcg acctcggctt cgtggcgatg aagggcttcg acgtcgagac
     5401 gtgggccccg ggcgtacagg aatggctgga ggtgtcgtcg acgagcgacg gcggctcgct
     5461 gcaggccgag cgcgcggcga tccgcttcaa gcgcgaggcc ggcggcaagg tggagcaccc
     5521 gcacctgctc aacgcgtcgg gcctcgcgct gccgcgcctc atgatctcgg tcatcgagaa
     5581 ctaccagcag caggacggat cgctcgtgat cccgaagccg ctgcgccagt acatgcgcgg
     5641 acgcgagcgc gtcgagccgg gagagttctc gctctagcgg gcggtcagct cttcgcgcgg
     5701 gactccggcg ccggtgtgtt ccgctgacgc tgaccggcga gcagcacgaa cagtccgacg
     5761 acgatcagca ccgcgggcca ccagcgcgcg accgcgtcgg ccgccgccgt gccaccgatc
     5821 gcctgcgacc cgccgaccac ggcgaggatg ccgcccggga tcagcggcca ccagtgcggg
     5881 tgggcgccga agaggttgtc gatcacgtag atcgcgagga agccggcgcc gaggccgagc
     5941 acgacggccc cgccgttcat gctgtagccg aagtcctcga ggcccacgcc gatggcgaga
     6001 ccgccgagga tcgcgccggg aacgaggaac ccgtcctgcc gcgtgccggc gtagaaggcg
     6061 aagagcagga tcgagatcga gccgagcacg atccagtccg catccttcgg ctggaagagc
     6121 tcgatcgcca gcaacacgag gccgataccg atgagagcaa ggcccgcgag ccattgccgc
     6181 tgcggtgtgg gcgtggtgtt cataggacac ctcccacgct caggctccct cccgctcggg
     6241 tcgctgccaa gtgccgttcg tcgcgaccgg tgtcccgtga acggacgaca cgcgtggcag
     6301 gccgatccag cgcgggacgc gtgcgacgta ggcgcggtac tcgtcgccga agatctgctc
     6361 caagcgcacc tcgtccgtac ggacgcgctc cgcgagccgc cgcgcctggc gcagcgccag
     6421 cagcagcgcg atcggcgagc gtgtcgcgcc gaagagcgcc gtgcagaaga cgaggtgccc
     6481 gaggtacatg gggttgcgcg tatacgcgta cggaccgtcg gtgaggagct tcacaggcgg
     6541 acgctcgaag ccgcgcggtc cggccgcgct gcgctcgcgg tgttcccccg ccgtgcggta
     6601 caggccgtag ccgagcgcga gcagcggcgc ccagcgcagg agtcctgcct cgcggtccag
     6661 ccacaggccg gcgatccggg gcacccgttc gatgtagccg accacgtcgg gctgcgtcgc
     6721 cgtgccggcc ga
//
LOCUS       DASTZR010000115         2400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_397246, whole genome shotgun sequence.
ACCESSION   DASTZR010000115 DASTZR010000000
VERSION     DASTZR010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397246"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..139
                     /locus_tag="VFV20_02645"
     CDS             <1..139
                     /locus_tag="VFV20_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012959290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HEU5287277.1"
                     /translation="RATLKRFFREKDRIRLQPANGQMQPIYTRDIQIQGKVIGVIRRL
                     A"
     gene            149..1345
                     /locus_tag="VFV20_02650"
     CDS             149..1345
                     /locus_tag="VFV20_02650"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEU5287278.1"
                     /translation="MPARRSSDLPILTLRELNRALLARQMLLRRDRVDPTSAIERLGA
                     LQAQWPRAPYVGLWSRLERFDRADLEDALRRRRVVKATLMRGTLHLASASDYPYYAIA
                     AREARRLLWAATERRFVAFFATQLPAVRAFAKHGRAAIADPEKLHERLIRYTRTPRSR
                     EDLIAFIAREADVPHELATHLVWGFVAAHGMLVHTPDSAHFAADRAGELVAASVALPR
                     MKVPPFPDAVRHTVTRHLAAFGPATVEDISSWTSIRTPPIREALAALGSRVRRFADER
                     GRVLHDLGDAPRPGPDTAAPVRFLPKWDSTLLAYTPAERVRILPERHRAAVIAKNGDV
                     SQTILVDGVVAGTWAITRTSRQAVVTVTPFGRLSRADRAALADEGERLARFLAPDLTS
                     HALHVA"
     gene            1342..>2400
                     /gene="dinB"
                     /locus_tag="VFV20_02655"
     CDS             1342..>2400
                     /gene="dinB"
                     /locus_tag="VFV20_02655"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU5287279.1"
                     /translation="MNAEPRSILHCDLDAFYASVEQRDHPEYRGKPVIVGGGPNDRGV
                     VSAASYEARKFGVRSAMPLRTASRLCPDGIFVPGDRERYEEASDAVMALFAERTPLVQ
                     PISLDEAFLDVTATEHLFGGDVAIAKRLKADVRAKLGLVISVGVATNKLCAKIGSDLR
                     KPDGLVIVPAGQEAAFLAPLELARLWGVGPKTRELLEGWGLRTIGDLARADVATLEAR
                     LGEHGRSIWERANGIDDEPVVPDMAPKSIGHEHTFDRDTLDVSTIEGTLLRLSEGVGR
                     RSRAQDLRGRTITLKLRVAPFETRSRQRTLVSPTNDDLTIYRVGRQLLRDALGEDRSS
                     GRTSPVRLVGISLSGLEVG"
BASE COUNT          331 a          900 c          826 g          343 t
ORIGIN      
        1 ccgggcgacg ctgaagcgct tcttccgtga gaaggaccgc atccgtctcc agccggcgaa
       61 cggccagatg cagccgatct acacccgcga catccagatc caagggaagg tcatcggcgt
      121 catccgccgc ctcgcctagc atcgcgccat gcccgcgcgg cggtcgtcgg accttcctat
      181 cctcaccctt cgcgagctga atcgcgccct cctcgcgcgc cagatgctgc tgcggcggga
      241 ccgtgtcgac cccacgagcg cgatcgagcg tctcggcgcg ctccaggcgc agtggccgcg
      301 cgcgccgtac gtcggcctct ggtcgcgcct cgaacggttc gatcgcgcgg atctcgagga
      361 cgccctgcgc cggcgccgcg tcgtgaaggc gacgctcatg cgcgggacgc tgcatctggc
      421 gagcgcctcc gactatccgt actacgcgat cgcggcgcgt gaggcgcgcc ggctgctgtg
      481 ggcagcgacg gagcgccgct tcgtcgcgtt cttcgcgacg cagctccccg cggtccgcgc
      541 cttcgcgaag cacgggcgcg cggcgatcgc cgatccggag aagctgcacg agcggctgat
      601 ccggtacacg aggacgccgc ggtcgcgcga ggatctcatc gccttcatcg cgcgcgaggc
      661 cgacgtcccg cacgagctcg cgacgcatct ggtgtgggga ttcgtcgccg cgcacgggat
      721 gctcgtccac acgcccgaca gcgcgcactt cgccgcggac cgtgcgggcg agctcgtcgc
      781 ggcgagcgtc gcgctgccac ggatgaaggt cccgccgttc cccgacgccg tgcggcacac
      841 cgtgacgcgg catctcgccg cgttcggtcc ggcgacggtc gaggacatct cgtcgtggac
      901 gtcgatccgc acgccgccga tccgcgaggc gctcgcggcg ctcggctccc gcgtccgccg
      961 gttcgcggac gagcgcggcc gcgtgctcca cgacctcggg gacgcaccgc ggcccggccc
     1021 cgacaccgcg gcgccagtgc ggttccttcc aaagtgggac agcaccctgc tcgcctacac
     1081 cccggccgag cgcgtgcgga tcctgcccga gcgtcaccgt gcggccgtca tcgcgaagaa
     1141 cggggacgtc tcgcagacca tcctcgtcga cggcgtcgtg gcggggacct gggcgatcac
     1201 gcggacctcc cgccaggcgg tcgtcaccgt gacgccgttc gggcggctgt cgcgcgccga
     1261 ccgcgcggcg ctcgcggacg agggtgaacg ccttgcgcgg ttcctcgcgc ccgacctcac
     1321 atcgcacgcg cttcacgtcg catgaacgcc gagccgcgct ccatcctcca ctgcgacctc
     1381 gacgccttct acgcgtcggt ggagcagcgt gaccaccccg agtaccgcgg caagcccgtc
     1441 atcgtcggcg gcggtccgaa cgaccgcggc gtcgtgagcg ccgcgtccta cgaggcgcgg
     1501 aagttcggcg tgcgctcggc gatgccgctc cgcacggcct cccgtctctg ccccgacggc
     1561 atcttcgtgc ccggcgatcg cgagcgttac gaggaagcgt ccgacgcggt gatggcgctc
     1621 ttcgcggagc gcacgccgct ggtgcaaccg atctcgctcg atgaggcgtt cctcgacgtg
     1681 accgcgacgg aacacctctt cggcggcgac gtcgcgatcg cgaagcggct caaggccgac
     1741 gtgcgcgcga agctcggtct ggtcatcagc gtgggcgtcg ccacgaacaa gctgtgcgcg
     1801 aagatcggct ccgacctgcg caagcccgac ggactggtga tcgtcccggc gggacaggag
     1861 gccgcgttcc tcgcgccgct cgagctcgcg cggctctggg gtgtcggccc gaagacgcga
     1921 gagctgctcg agggctgggg cctgcgcacg atcggcgacc tggcgcgcgc cgacgtcgcg
     1981 acgctcgagg cgcgactcgg agagcacggc cgttcgatct gggagcgcgc gaatggcatc
     2041 gacgacgagc cggtcgtgcc ggacatggcg ccgaagtcga tcggccacga gcacaccttc
     2101 gaccgcgaca ccctcgacgt ctcgacgatc gaagggacgc tcctccggct ttccgagggc
     2161 gtgggtcgga gatcgcgcgc acaggacctg cgcggccgga cgatcacgct caagctccgc
     2221 gtcgccccgt tcgagacgcg gtcgcgccag cgcacccttg tctcgccgac gaacgacgac
     2281 ctcaccatct accgggtcgg ccgtcagctg ctgcgagacg ccctcggtga ggatcgctcc
     2341 agcggacgca ccagcccggt ccgcctcgtc gggatctcac tctctggact cgaggtcggc
//
LOCUS       DASTZR010000116         9492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_633782, whole genome shotgun sequence.
ACCESSION   DASTZR010000116 DASTZR010000000
VERSION     DASTZR010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9492
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633782"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VFV20_02660"
     CDS             <1..523
                     /locus_tag="VFV20_02660"
                     /inference="COORDINATES: protein motif:HMM:NF015298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEU5287280.1"
                     /translation="VDQSVAVQPIAMPIAIPNARGLAFTVAADLTGEPAPEMVPQPEP
                     TPDPTPSPTPRPTPPPPPPPAYAVMPNGMPGVVVTASWYGPGFYENRLPCWQWLQANG
                     LPIQFLPDTWGVAQKTLPCGTMLTLSHGTNVITVPVVDRGPYVAGRELDLSPRVKAAL
                     GCTDLCTVVMHIP"
     gene            660..998
                     /locus_tag="VFV20_02665"
     CDS             660..998
                     /locus_tag="VFV20_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein B"
                     /protein_id="HEU5287281.1"
                     /translation="MTRNKGAMTVAEAGRRGGATTAQRHGPEFYEQIGKKGGRTTAQR
                     HGSDFYEQIGKQGGQKVKELYGPRFYGRIGKIGGDTVSEKYGHEHFEEIGKKGGQKVA
                     ELIARGRQAS"
     gene            1227..1511
                     /locus_tag="VFV20_02670"
     CDS             1227..1511
                     /locus_tag="VFV20_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287282.1"
                     /translation="MTTPLVAVEWEDITAYSRTAIPDDFEPFRLRKLTCGLLWKETAE
                     YVVVVGDYDLTNEGKDYRHNDFHIIPRGVISKITYLSDAGTLVSLQVESA"
     gene            1623..1877
                     /locus_tag="VFV20_02675"
     CDS             1623..1877
                     /locus_tag="VFV20_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287283.1"
                     /translation="MSVRAELYCYFCGHGLGEVLVPTKARKPSVEQLRAAYSQPGREA
                     PAWSAADQPLCPRCGGQLFLERFEHEVVRAHDRRALRRAS"
     gene            complement(2213..2767)
                     /locus_tag="VFV20_02680"
     CDS             complement(2213..2767)
                     /locus_tag="VFV20_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEU5287284.1"
                     /translation="MTRDEAWALLCEHTKGDGLRKHALAVEAAMRHFAAKFGEDEEVW
                     GVTGLLHDFDYERDPAGHPQNGKPILEQAGVPGNIVHAIQSHGDHTGVPRVTPMEKTL
                     YAVDELTGFITAVALVRPSKAVRDVDAAAVRKKMKDKAFARAVNREAMTAGAADLQVP
                     FEELVAEIVVALGRVGDRLGLAGV"
     gene            2815..3147
                     /locus_tag="VFV20_02685"
     CDS             2815..3147
                     /locus_tag="VFV20_02685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEU5287285.1"
                     /translation="MIEFAPGCAEKLRELRGDDPSRAFLRLYVAGRSCSGYQYGLAFD
                     SSADESDQVIEAAGIPVAIDSMSQPYCDGATIEYVDEEQYPGGGFRVTNAKFGGDGGC
                     GGSCSCGH"
     gene            3193..3378
                     /locus_tag="VFV20_02690"
     CDS             3193..3378
                     /locus_tag="VFV20_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287286.1"
                     /translation="MTGCGCALLIALIVGLITFMVFGSTDPGEPVDAAVALLAALSAL
                     GVAQIPIRRALVRARGA"
     gene            complement(3316..4755)
                     /locus_tag="VFV20_02695"
     CDS             complement(3316..4755)
                     /locus_tag="VFV20_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEU5287287.1"
                     /translation="MKERSLTSPDDTRPYHRVIDELALLPMHEAAALLRARRASSVEL
                     TRACIERIDRLDSDLVAFITRTADAALADAAAADTDLARGVDHGPLHGMPVALKDLFD
                     VTGVRTTGGSRILADNVPQRSSAVAERLRAAGAVSLGKTNLHEWAFGVTNQNPHFGAA
                     KNPWDPSRIPGGSSGGTAIAVATGMCLMSPGSDTGGSIRIPASLCGVAGLKPTYGRVS
                     LRGVVALSWTLDHAGPLARSVRDLASTLQVLAGYDAEDPGSADTPVDDYLADIESGAR
                     GLRVLVPTDHFFTDCDAEVEAAVREAVELLRREGATIVERTPPDLALLAAQRPIISVD
                     AATYHREHLRDRSGDIGADVLERLRYGTTVDALDYARARRDRETIRRSWTRMLREVDV
                     IATPTTRIAAPPREGQDAVAAAARLTANTSPFNLTGLPALSIPCGFTRAGLPIGLQLA
                     AAPWREAVLLRAARAYERATDWNLRHPQG"
     gene            4736..5362
                     /locus_tag="VFV20_02700"
     CDS             4736..5362
                     /locus_tag="VFV20_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287288.1"
                     /translation="MSDRSFIRFGGLAGILLALTSWAAVVVYFTSARAGEDLPGLETF
                     RLLYALVAVWALFGIVAVHWVVRANGEAWSFFATLVGAIAAAGTIAAQLYQVAGLRAA
                     ILDQGTKPPTISPTDPFSVMSFALTGLWFLIAGLLLWRARYPRPIAALGLLAAAVLFA
                     GFLGALSAQDGLVTMAALVAGAIGSPVYWLSLGVAMRRRSATVDRPAR"
     gene            5359..6081
                     /locus_tag="VFV20_02705"
     CDS             5359..6081
                     /locus_tag="VFV20_02705"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HEU5287289.1"
                     /translation="MTEERDPITADHQRIRGELERRVRAALERGTRNDLDGLVALVEG
                     ELLGHARAEQRYLYPLMDELVRDHGRATATMQIDHEAIAAHARSVAEAVERLRVADDR
                     MSRMESRAALRDALLRLEAILGVHLEKEERVYLPLVAAHLSADEQRALAERMHGVEAT
                     EHAPVEIDARAIPQRERHARIFDAFDRLPIGGALVLVNDHDPRPLSYQFETKRPGGFT
                     WEYLERGPLWRVRITRGAPPRR"
     gene            6210..7427
                     /gene="nirK"
                     /locus_tag="VFV20_02710"
     CDS             6210..7427
                     /gene="nirK"
                     /locus_tag="VFV20_02710"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HEU5287290.1"
                     /translation="MSSITHETARTPETRGSDLPSAFTGALFGALAIGVATALVVGVL
                     GATGNIRSGAASGAAANTDATTAPGMSTMPGMGTIPQTGAGAQVTLPAAKAVTAKTIA
                     RPATEVTAPVNRAAPATVRVDLTTQEVVAEIEKGETYTYWTFNGLIPGPMVRVRVGDT
                     VELHLKNSSSSIMAHSIDLHAVTGPGGGATATQTMAGQETMVTFKALNPGAYIYHCAT
                     APIPQHIANGMYGMIIVEPEGGLPKVDREFYVMQGELYTTARLGTPGHHDFDAVKMDN
                     EQPDYVLFNGKVGSLTGDNAMKAQVGETVRIFFGVGGFLSSNLHLIGEIFDKVYPEGA
                     VGSPANVNVQTTNVPAGGATIVEFKVNVPGTYTLVDHALPRALDKGAAAQLVVTGAED
                     KTIYSGPLSGAGH"
     gene            complement(7490..8923)
                     /locus_tag="VFV20_02715"
     CDS             complement(7490..8923)
                     /locus_tag="VFV20_02715"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU5287291.1"
                     /translation="MRTAVAVLAILVLAAPPALAVEPTDDGEPEPSLHCVQPVATTRV
                     ENVMEAQWSPDGHTLALVWFARVPSTRSPTGYREVEVVDSLDVRTGRLWPIGVGDEVD
                     WSGTGAYLSYWGPNADELRVVHGDRIVARLAPTIPRVRWVGDGLLFIEKNTIREWRDG
                     AVRTIAVIAQKYVPRYPRDDVYFSGDGEQITLTRYAQDGTLERFLGTTLTGDFVPLDT
                     GDARYIEWAPAGHTLLVRDVDRIELRDPIAGTKTITGARAARAVHQWTPDGRTLLLGS
                     VSPTVPGGDAFDAFRPWDAGGGAPVATLPNVLGARAFSLDGRYFAGVSRTGAHTTRLE
                     VYRCAGVPAMALPSPDAAERLARIDAGPGRFVRPTAGEISQFLQGSHTGIDLATPFGS
                     LITADDDGFVSFVEWVNTGGNRVCVQHRGALESCVYHTSLPLVGVGDRVVRGQPIALI
                     GMTGVVTGPHTHWEVKLANRIVDPLTF"
     gene            complement(9007..9216)
                     /locus_tag="VFV20_02720"
     CDS             complement(9007..9216)
                     /locus_tag="VFV20_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287292.1"
                     /translation="MQPRCDILSVLRPSQLARHGIGEHDVGPDPYVVNEPKSFGGRTL
                     HFCSLAHLAWWVRVAAVGADKRITH"
     gene            complement(9226..9302)
                     /locus_tag="VFV20_02725"
     tRNA            complement(9226..9302)
                     /locus_tag="VFV20_02725"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9266..9268),aa:Asp,seq:gtc)
BASE COUNT         1394 a         3368 c         3331 g         1399 t
ORIGIN      
        1 ggtcgaccag tccgtggccg tccagcccat cgcgatgccg atcgcgatcc cgaacgcgcg
       61 cggcctcgcc ttcacggtcg ccgcggacct tacgggggag ccggcgccgg agatggtccc
      121 gcagcccgag cccacgccgg atcccacgcc ctcgccgacg ccacggccga cgcccccgcc
      181 gccgcctccg ccggcgtacg cggtcatgcc gaacggcatg ccgggcgtgg tggtcaccgc
      241 ctcctggtac ggaccggggt tctacgagaa ccggctgccg tgctggcagt ggctccaggc
      301 gaacggactg ccgatccagt tcctcccgga cacgtggggc gtggcgcaga agacgctgcc
      361 ctgcggcacg atgctcacgc tctcccacgg caccaacgtc atcaccgtgc cggtcgtcga
      421 tcgcggcccg tacgtggccg ggcgtgagct cgatctgtca ccacgggtca aggccgcgct
      481 cgggtgcacg gacctgtgca cggtcgtgat gcacattccc tagcgatcgt ccggcatacg
      541 tacgcctggc ctcccgtccg ttgtgctgcc taccgagcgg tggcacggga cttgcagcct
      601 aggcgggtga ccggggaagc attcggcaaa ccccggacgc caaacaaggg agaggtgtca
      661 tgacgaggaa caagggcgcg atgaccgtcg cagaggccgg ccgccgcggc ggcgctacca
      721 ccgcacagcg ccacggtccc gagttctacg agcagatcgg caagaagggc ggccgcacga
      781 cggcgcagcg tcacggctcc gacttctacg agcagatcgg caagcagggc ggacagaagg
      841 tcaaggagct gtacggtccg cgcttctacg gccgcatcgg caagatcggc ggcgacacgg
      901 tctccgagaa gtacggtcac gagcacttcg aagagatcgg caaaaagggc ggccagaagg
      961 tcgcggagct catcgcccgc ggtcgtcagg cgagttagcc gctcgtcagc ttgtccgtct
     1021 ccctcacgtg cggacgatcc acggctgacg tggaccgatc ccacgttgac atcgcagatg
     1081 gtgccgtcac gccgttgaca cgtcggtggc gacttttatc gtcaccgact gtacgcggcc
     1141 gtgacgatcg cgagcgtccg tgtagcgaag accggacgga tcgcgtgcga cggcagaggg
     1201 ggaaagcgtt ggagaccggt gggaacgtga cgaccccgct cgtagcggtc gagtgggagg
     1261 acatcacggc gtactcccgt accgcgatcc ccgacgactt cgagcccttc cggcttcgca
     1321 agctgacctg cggcctgctc tggaaggaaa cggctgagta cgtagtcgtg gtgggcgact
     1381 acgacctcac caacgaaggc aaggactacc ggcacaacga cttccacatc atcccccgtg
     1441 gcgtgatcag caagatcacc tacctctccg acgcgggcac gctcgtcagc ctccaggtcg
     1501 agtcggccta gcctcgtcct ttcgtagccc ggttaacgga acgtccgtgc ccggcgaggg
     1561 gtactggaac aaggcgagga ctggcaccgt tcttgtcatc ggaatcacct ggaggtcccg
     1621 ggatgtccgt tcgtgctgag ctctattgct acttctgcgg tcacggtctc ggcgaggtgc
     1681 tcgtgccaac gaaggcgcgg aagccctcgg tcgagcagct tcgtgccgcc tattcgcagc
     1741 ccggccgcga agcgccggcg tggagcgctg ccgatcagcc gctctgcccc cgctgcggcg
     1801 gccagctgtt cctcgagcgc ttcgagcacg aggtcgtgcg cgcgcacgac cggcgcgcac
     1861 tgcgccgcgc gagctgatcc tcgcgacgcg acacgctttg ccggacggcc gggacgcaag
     1921 tcccggccgt tcttgttcgc gtgtcggtgc gtgacgtcgg ctggttcgtc ctgggtggcg
     1981 tctgcggatg gagctgggcg cgcggcgtca tcgacgcgca cgtacggcgg ccgcgcgtca
     2041 gccgccggca ggggcagatc ctcgcgctcg tcgcgcgcgg aatgagctcg aaggagatcg
     2101 cgcgtcacga gcgcatcagc gagcactccg tgaacacgca catccgccgt gcctgccgca
     2161 cgctcggcgt gccctcgcgc gccgcggccg tggccgccct gcgcggcggg gctcagaccc
     2221 ccgcgagccc cagacgatcc ccgacgcgac ccagcgcgac gacgatctcg gcgacgagct
     2281 cctcgaacgg cacctgaaga tccgccgcgc ccgccgtcat cgcttcccgg ttgacggcgc
     2341 gcgcgaacgc cttgtccttc atcttcttgc ggacggcggc ggcgtccacg tcacgtacgg
     2401 ccttcgacgg ccgcaccagc gcgacggcgg tgatgaagcc cgtcagctca tccaccgcgt
     2461 agagcgtctt ctccatcggc gtgacgcgcg gcacgccggt gtggtcgccg tgcgactgga
     2521 tggcatgcac gatgttgccg ggcacgcccg cctgctcgag gatcggcttt ccgttctgcg
     2581 gatggcccgc ggggtcgcgc tcgtagtcga agtcatggag cagtccggtg acgccccaga
     2641 cctcctcgtc ctcgccgaat ttcgcggcga agtgccgcat ggccgcctcg accgccagcg
     2701 cgtgcttgcg cagcccgtcg cccttggtgt gttcgcagag cagcgcccac gcttcgtcgc
     2761 gcgtcatcgg gctcctccct cgtctggacc tgccgcggat gctaggcttt tggcgtgatc
     2821 gagttcgcac caggatgcgc cgagaagctg cgcgagctgc gcggcgacga cccgtcgcgt
     2881 gcgttccttc gcctgtacgt ggcggggcgc agctgcagcg gctatcagta cgggctcgcg
     2941 ttcgactcga gcgccgacga gagcgatcag gtgatcgagg ccgccggcat cccggtcgcg
     3001 atcgactcca tgagccagcc gtactgcgac ggcgcgacga tcgagtacgt cgacgaggag
     3061 cagtacccgg gcggcggctt ccgcgtcacg aatgcgaagt tcggtggcga tggtggctgc
     3121 gggggctcct gcagctgcgg gcactaacac ccgcgtagca gcttccattt cgtcagcccg
     3181 gcgagcgtcg gcgtgaccgg ctgcggctgc gcgctcctca tcgccctcat cgtcgggctc
     3241 atcacgttca tggtgttcgg ctcgaccgat ccgggcgagc cggtcgacgc cgcggtcgcg
     3301 ctgctcgcgg ccctctcagc cctgggggtg gcgcagattc caatccgtcg cgcgctcgta
     3361 cgcgcgcgcg gcgcgtagca gcaccgcctc gcgccacggc gcggcggcga gctgcaatcc
     3421 gatcggcagg cccgcgcgcg tgaagccgca cgggatcgac agcgcgggaa gcccggtgag
     3481 gttgaacggc gaggtgttcg ccgtgaggcg cgcggccgcg gcgacggcgt cctggccctc
     3541 gcgcggcggt gcggcgatcc gtgtcgtggg cgtcgcgatg acgtcgacct cgcgcagcat
     3601 ccgcgtccag gatcgccgga tcgtctcgcg atcgcgccgc gcacgcgcgt agtcgagcgc
     3661 gtccacggtg gtgccgtagc gcagccgctc gaggacgtcc gcgccgatgt cgccggagcg
     3721 gtcgcggaga tgctcgcggt gatacgtggc cgcgtcgacg gagatgatcg gacgctgcgc
     3781 cgcgagcagc gcgagatcgg gcggcgtacg ttccacgatc gtcgcgccct cccgccgcag
     3841 cagctcgacc gcctcgcgca cggccgcctc gacctcggcg tcgcagtccg tgaagaagtg
     3901 gtcggtgggc accaggacgc gcagcccccg cgccccgctc tcgatgtccg cgaggtagtc
     3961 gtcgaccggc gtgtccgcgc tgccggggtc ctccgcgtcg tagccggcga ggacctgcag
     4021 cgtcgacgcg aggtcgcgca ccgatcgcgc gagcgggccg gcgtggtcga gcgtccacga
     4081 cagcgcgacg acgccgcgca ggctgacgcg accataggtg ggcttgaggc ccgcgacgcc
     4141 gcacagcgac gcggggatgc ggatggagcc gcccgtgtcg ctgcccggcg acatgaggca
     4201 catccccgtc gcgaccgcga tcgcggtccc accgctggag ccgcccggga tgcgcgaggg
     4261 gtcccacggg ttcttcgccg caccgaagtg ggggttctgg ttggtgacgc cgaaggccca
     4321 ctcgtggagg ttcgtcttgc ccagcgacac cgcgccggca gcgcggagcc gctccgcgac
     4381 ggcgctcgag cgctgcggca cgttgtcggc gaggatgcgc gacccgccgg tggtgcggac
     4441 gccggtcacg tcgaagagat ccttgagggc gacgggcatg ccgtgcagcg gtccgtgatc
     4501 gacgccgcgc gcgaggtccg tgtcggccgc cgccgcgtcg gcgagcgccg cgtcggccgt
     4561 gcgcgtgatg aaggcgacga ggtccgagtc gagccggtcg atgcgttcga tgcacgcacg
     4621 ggtgagctcg accgacgacg cgcgtcgcgc gcgcagcagc gcggccgcct cgtgcatcgg
     4681 gaggagcgcg agctcgtcga tgacgcgatg gtacgggcgt gtatcgtccg gcgacgtgag
     4741 cgatcgctcc ttcatccgtt tcggcggcct cgccgggatc ctcctcgcgc tcacgtcgtg
     4801 ggccgcggtc gtcgtgtact tcacgtcggc gagggcgggc gaggatctgc ccggcctcga
     4861 gaccttccgt ctgctgtacg cgctggtcgc cgtgtgggcg ctcttcggca tcgtggcggt
     4921 gcactgggtc gtgcgtgcga acggtgaggc gtggtcgttc ttcgccacgc tcgtgggcgc
     4981 gatcgccgcc gcggggacga tcgccgcgca gctctatcag gtcgcggggc tccgtgccgc
     5041 gatcctcgac cagggcacga agccgcccac gatcagcccg accgacccgt tctcggtcat
     5101 gagcttcgcg ctcaccgggc tctggttcct catcgccggt ctgctgctct ggcgcgcgcg
     5161 atacccgcgg cccatcgccg cgctcggact cctcgcggca gcggtgctgt tcgcgggctt
     5221 cctcggcgcc ctgtcggcgc aggacggcct cgtgacgatg gccgcgctcg tcgccggcgc
     5281 gatcggtagt cccgtttact ggctgtcgct cggcgtggcc atgcggaggc gttccgcgac
     5341 cgtggaccgt ccggcacgat gaccgaggag cgggacccga tcaccgccga ccatcagcgc
     5401 atccgcggcg agctcgaacg ccgtgtgcgc gcggcgctag agcgcggcac ccgcaacgac
     5461 ctcgatgggc tcgtcgcgct cgtcgaggga gagctgctcg ggcacgcgcg cgccgagcag
     5521 cggtatctgt atccgctcat ggacgagctc gtccgcgacc acggccgcgc gacggcgacc
     5581 atgcagatcg accatgaggc catcgccgcg cacgcgcggt ccgtcgccga ggcggtggaa
     5641 cggctccgcg tggcggacga ccgcatgtcc cggatggagt cacgggccgc gcttcgcgac
     5701 gcgctgctgc gcctcgaggc gatcctcggc gtgcacctcg agaaggagga gcgcgtctac
     5761 ctaccgctcg tcgccgcgca tctctccgca gacgagcagc gtgccctcgc cgagcgcatg
     5821 cacggcgtcg aggcgaccga gcacgctccc gtcgagatcg atgcgcgcgc catcccccag
     5881 cgcgagcgcc acgcgcgcat cttcgacgcg ttcgaccgcc tgccgatcgg cggcgccctg
     5941 gtgctcgtga acgatcacga cccccgcccg ctctcgtacc agttcgagac gaagcgtccc
     6001 ggcgggttca cgtgggagta cctcgagcgc ggccccctct ggcgcgtccg catcacccgc
     6061 ggcgcgcccc cgcgacgctg atcgcctgag cagcgggccc ttgggccccc gaacttggga
     6121 ccttcggtcc cttcctccgc atggcgtctc aacacacttt tgacgcaatt cgagggcgga
     6181 cagcgccccg attacggagg tcacctgaca tgagctcgat cacgcacgaa acggcgcgca
     6241 cacccgaaac gcgcggcagc gacctgccga gcgccttcac cggtgcgctc ttcggcgcac
     6301 tcgcgatcgg cgtcgcgacg gcactcgtcg tcggcgtcct cggcgcgacc ggcaacatcc
     6361 gctccggggc cgcgagcggg gcggccgcga acaccgacgc caccaccgcg cccggcatga
     6421 gcacgatgcc cgggatgggc acgatcccgc agacgggcgc cggcgcgcag gtcacgctcc
     6481 ccgcagccaa ggcggtcacc gcgaagacca tcgcgcgtcc ggcgaccgag gtcaccgcgc
     6541 ccgtgaaccg cgccgccccg gcgacggtcc gcgtggatct caccacgcag gaggtcgtcg
     6601 ccgagatcga gaagggcgag acctacacgt actggacgtt caacggtctc atccccggcc
     6661 cgatggtccg cgtgcgcgtc ggcgacaccg tcgagcttca cctgaagaac tcgtcgtcca
     6721 gcatcatggc gcactcgatc gacctgcacg ccgtgaccgg cccgggcggc ggcgcgaccg
     6781 cgacgcagac gatggcgggc caggagacca tggtcacgtt caaggcgctg aaccccggcg
     6841 cgtacatcta ccactgcgcg acggcgccga tcccgcagca catcgcgaac ggcatgtacg
     6901 ggatgatcat cgtcgagccc gagggcgggc tgcccaaggt cgaccgcgag ttctacgtca
     6961 tgcagggtga gctctacacc acggcgcgcc tgggcacccc cggccaccac gacttcgacg
     7021 cggtcaagat ggacaacgag cagcccgact acgtcctctt caacggcaag gtcggatcgc
     7081 tcaccggcga caacgcaatg aaggcgcagg tcggcgagac cgtccgcatc ttcttcggcg
     7141 tcggcggctt cctgtcctcg aacctgcacc tcatcggtga gatcttcgac aaggtctacc
     7201 ccgagggcgc cgtcggcagt cccgcgaacg tgaacgtgca gaccacgaac gtgccggcgg
     7261 gcggcgcgac gatcgtcgag ttcaaggtga acgtgcccgg cacgtacacc ctggtggacc
     7321 acgcgctgcc tcgcgcgctc gacaagggcg cggccgcgca gctcgtcgtc acgggtgccg
     7381 aggacaagac gatctacagc gggcccctca gcggagccgg ccactagaca ccgacagatc
     7441 cgcgacctcc gtgaaagaac ggccgggaac cccggccgtt ctttcgtgct cagaaggtga
     7501 gcgggtcgac gatccggttc gccagcttca cttcccagtg cgtgtgcggg ccggtgacca
     7561 ctccggtcat gccgatgagc gcgatgggtt ggccgcgtac cactcggtcc cccacgccca
     7621 cgagcgggag gctggtgtgg tagacgcagc tctcgagcgc accgcggtgc tgcacgcaca
     7681 cgcggttgcc gcccgtgttg acccactcga cgaacgacac gaagccgtcg tcgtcggcgg
     7741 tgatgagcga gccgaacggc gtcgcgaggt cgatgccggt gtgcgatccc tggaggaact
     7801 gcgagatctc gccggcggtg gggcgcacga agcggccggg cccggcgtcg atgcgcgcga
     7861 gccgctcggc cgcgtccggc gacgggagtg ccatcgcggg cacgcccgcg cagcggtaca
     7921 cctcgagtcg cgtcgtgtgc gcgccggtgc gcgagacgcc ggcgaagtac ctgccgtcga
     7981 ggctgaacgc acgcgccccg aggacgttcg gcagcgtggc gaccggcgct ccgccaccgg
     8041 cgtcccacgg ccggaaggcg tcgaacgcgt caccgccggg gacggtgggg gacacggatc
     8101 ccagcaggag cgtccgtccg tcgggcgtcc actggtgcac ggcgcgggcg gcgcgcgcgc
     8161 cggtgatcgt cttcgtgccc gcgatcggat cgcgcagctc gatgcggtcg acatcgcgca
     8221 cgagcagggt gtgaccggcc ggcgcccact cgatgtagcg ggcgtcgccg gtgtcgagcg
     8281 gcacgaagtc gccggtgagc gtcgttccca ggaagcgctc gagcgtcccg tcctgcgcgt
     8341 agcgcgtcaa cgtgatctgc tcgccgtcgc cgctgaagta cacgtcgtcc cgcgggtacc
     8401 ggggaacgta cttctgcgcg atcaccgcga tcgtgcggac ggccccatcc cgccactcgc
     8461 ggatggtgtt cttctcgatg aacaagagac cgtcgccgac ccaccgcacg cgcgggatcg
     8521 tcggcgcaag ccgcgcgacg atgcgatcgc cgtggaccac gcgcagctcg tcggcgttcg
     8581 gtccccagta cgagaggtac gcgcccgtgc ccgaccagtc gacctcgtcg ccgacgccga
     8641 tcggccacag ccggccggtg cgtacgtcga gcgagtcgac gacctcgacc tcgcgatacc
     8701 cggtggggct tcgtgtcgat ggaacgcgcg cgaaccacac cagcgcgagg gtgtgcccgt
     8761 ccggcgacca ctgggcctcc atgacgttct cgacccgcgt cgtggcgacg ggctgcacgc
     8821 agtggaggga cggctcgggc tcgccgtcat cggtcggctc gaccgcgagg gccggcggcg
     8881 ctgcgagcac gaggatcgcg aggacggcga ccgcggtgcg catcacatat gagtgtgcgg
     8941 ctccggggcg gtgctaccag cacgtccgtt cgtatcgcct ccggtacagg ggcggcgtgc
     9001 gcggggtcag tgcgtgatgc gcttgtcggc gccgacggcc gcgacccgga cccaccacgc
     9061 gaggtgcgcg agcgagcaga agtggagcgt gcggccgccg aaggacttcg gctcgttgac
     9121 gacgtacgga tccggcccga cgtcgtgctc gccgatgcca tgtcgcgcga gctgcgaggg
     9181 acgcaggacg gagaggatgt cacaacgcgg ctgcatcatc ggatctggcg gtcccgaccg
     9241 gattcgaacc ggcgatctcg tccttgacag ggacgtatgc taggccgctg caccacggga
     9301 ccatgcgcgg aaaagcgcac tcgcgctccc ctatgcgccc tcacattctg acagataccc
     9361 cctttcttgt ggcccagccg atcccgcctc ggtttcgcgc gcaggcggcg tgcgtccgtg
     9421 tctggcgagc ggcgagtact ccctcgacga gcacctgcgc ccagcgggcc cggacgcgac
     9481 gagggtcgtg at
//
LOCUS       DASTZR010000117         6235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_503233, whole genome shotgun sequence.
ACCESSION   DASTZR010000117 DASTZR010000000
VERSION     DASTZR010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6235
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503233"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="VFV20_02730"
     CDS             complement(<1..932)
                     /locus_tag="VFV20_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HEU5287293.1"
                     /translation="MTTARYPGIPAVADGSEAVAHVEIRLAEAACAYPITPSTTMAAL
                     YQAAVADGRTDLWGTPIRFLEPESEHSSASAAEGFALAGGRVTNFTAGQGLILMKEVL
                     YVIAGKRLPAVFHVGARALTSHSLNIHAGHDDVMGVADTGWGILFARNVQEAADLAAI
                     ARRVAEKAEVPFIVAQDGFLTTHTFENVLLPEDELLREFLGAPRERVRSYFDPADALM
                     TGVVQNQDSYMKGKIGQRAFYHRLAEEVRLAMHEWATLTGRPYGLIDAYRCDDAETIV
                     VAMGTIADTAVAVVDALRAAGRRVGCVGVTSFRP"
     gene            complement(1032..1550)
                     /locus_tag="VFV20_02735"
     CDS             complement(1032..1550)
                     /locus_tag="VFV20_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEU5287294.1"
                     /translation="MIVPSVPVWEIVLRTVVVYVAVFALLRAAGKRELGQMSVPDLVV
                     ILVIANAVQNSLNGGDVSLTGGLVAAATLVALNVALDRFGRRLPFFSRFVVEEPTLLL
                     QDGKPLLEDMEREGVSLEDIEMSAREHGIADVADVSAAILEPDGSISIIPRAGGKMHR
                     SQRRIRQFRQRP"
     gene            complement(1604..2545)
                     /locus_tag="VFV20_02740"
     CDS             complement(1604..2545)
                     /locus_tag="VFV20_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5287295.1"
                     /translation="MRILLATDFSSSAEMAHTLVKGMALPANSQVRVVHAIEPITTVA
                     LFAPSALLTITDAAEAEARAQVRAAAKSLERDGVEVTSTVGYGRAADVIIDECAAFAA
                     DLLVVGSRGRGGIAASVLGSVSAELVDRAPCPVLVARRSTLERILLAEDGTTTSAIGA
                     RVIAGLPPLAKLPVHVVSVVDVPFPILLADPTGTSAAVEAYRAYEESMPRLRASHAAF
                     ASERAQALELLGTTATSEQREGDAAYELIAAAKERDADCIVVGSRGQTGLRRLVIGSV
                     ARGVLFHAPCSVLIAHPVKADAAAAGPSRAQPVGARS"
     gene            2722..3021
                     /locus_tag="VFV20_02745"
     CDS             2722..3021
                     /locus_tag="VFV20_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287296.1"
                     /translation="MGPLAFISLVVIGLVIGFAVQLLGKSKIRYEWLIVAVAITFGGY
                     FASESFPGSTVFEDIKDWGPAFDGLHVIPAAIGAIILGVVADLGMRTSPETQPGY"
     gene            3185..3442
                     /locus_tag="VFV20_02750"
     CDS             3185..3442
                     /locus_tag="VFV20_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287297.1"
                     /translation="MSTSVAAPEILVRVELTCFYCGHGYGEFKVRTTGRPTYRDLRRA
                     IEEAAPANPPAWDERGEPRCPRCGGKLFVEESDRRRSPVRG"
     gene            complement(3439..4527)
                     /locus_tag="VFV20_02755"
     CDS             complement(3439..4527)
                     /locus_tag="VFV20_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287298.1"
                     /translation="MTSRVPVAVLAALALIAAGWGGLSRLGLAPAAPTDVATYHGALM
                     VSSFIGTLIGLERAIVMRRRWSYAAPILSASAAVALIVGAGGGTAGLLVAGAALAFLA
                     GTVIGAPSSPGTAIVVMSAGAVAWLVGAVLWWQGSAAFRSMPWWSAFLILTIAAERME
                     LARALRPSGTASAILVLAATTFAAGTMLTLVDLAIGLRIAGAGALVLGLWLALRDRPP
                     AAARMSGLPRFIATAMPLAYAWLAVAAFFMITYDGIPSGLRYDAMVHALFAGFVMSMI
                     FAHGPIVVPAVIGTAIAYRRALYAPLAILEVSVALRVIGDALGRGDVRDAGAIGIALA
                     VVSFMAVMGTSIRMRRQASRRALREVSS"
     gene            complement(4532..5035)
                     /locus_tag="VFV20_02760"
     CDS             complement(4532..5035)
                     /locus_tag="VFV20_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEU5287299.1"
                     /translation="MTAGAMAPLREEHRELFPNIERLSAVADEVGRTDGAALRAKLDE
                     SYTFLAHQLVPHAKAEDEVLYPEVARLLGGPRATATMSRDHVEVVALISELATLRSFH
                     GEPDGERQNALRRVLYGLHALVRTHFAKEEEIYVPLLDDRLTMDEGRDLIRRMEAAAA
                     RLKATTS"
     gene            complement(5032..5310)
                     /locus_tag="VFV20_02765"
     CDS             complement(5032..5310)
                     /locus_tag="VFV20_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HEU5287300.1"
                     /translation="MATQIKDTLIDTFKLDRENVDKVIGKIEQLIHEGNVRRVVVKDA
                     QGTTIIEAPLTFGVVGVALVPVWAAIGAIAALAADFTIQVERKSEVEP"
     gene            complement(5331..5537)
                     /locus_tag="VFV20_02770"
     CDS             complement(5331..5537)
                     /locus_tag="VFV20_02770"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEU5287301.1"
                     /translation="MSDEIRIESESEIEIDPVCGATVQLEDAAQHSLAIDYEGREYTF
                     CGPDCRKRFEHSPARYAVPGREQP"
     gene            5619..6044
                     /locus_tag="VFV20_02775"
     CDS             5619..6044
                     /locus_tag="VFV20_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020104890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEU5287302.1"
                     /translation="MAVRHFARQGRRRRLHRMGTPKERLEAMLAELRAEVGEDIANLA
                     RDIDSRGEDTTPSQHPADVASDLYAREELVVHELALAHEVEEIEGALRRIAAGTYGRC
                     EECGGTVDPARLEARPQAALCITCQRRREDRRGRSLARA"
BASE COUNT          958 a         2203 c         2218 g          856 t
ORIGIN      
        1 aaggggcgga agctcgtcac accgacgcat ccgacgcggc gcccggccgc gcgaagcgcg
       61 tcgacgacgg caacggcggt gtcggcgatc gtgcccatcg cgaccacgat cgtctccgcg
      121 tcgtcgcagc ggtacgcgtc gatgagcccg tacggacgtc ccgtgagcgt ggcccactcg
      181 tgcatcgcga ggcggacctc ctcggcgagg cggtgataga aggcgcgctg cccgatcttg
      241 cccttcatgt acgagtcctg gttctggacg acgccggtca tgagcgcgtc ggcgggatcg
      301 aagtacgagc ggacccgctc gcgcggcgcg ccgaggaact cgcgcagcag ctcgtcctcg
      361 ggcagcagca cgttctcgaa cgtgtgcgtc gtgaggaaac cgtcctgcgc gacgatgaac
      421 gggacctcgg ccttctccgc gacgcggcgc gcgatggccg cgagatccgc ggcctcctgc
      481 acgttgcggg cgaagaggat cccccacccc gtgtccgcga cgcccatcac gtcgtcgtga
      541 ccggcgtgga tgttcagcga gtggctggtg agagcgcgcg cgccgacgtg gaacaccgcc
      601 ggcaggcgct tgcccgcgat gacgtacagg acctccttca tgaggatgag gccctgcccc
      661 gccgtgaagt tcgtgacgcg cccgcccgcg agcgcgaagc cctcggcggc cgacgccgat
      721 gaatgctcgg actcgggctc gaggaagcgg atcggcgtgc cccagaggtc cgtccggccg
      781 tcggcgaccg ccgcctggta gagcgcggcc atcgtggtcg agggcgtgat ggggtacgcg
      841 cacgcggcct ccgcgagccg gatctcgacg tgcgcgacgg cctcggagcc gtccgcgacc
      901 gcgggaatgc ccggatagcg tgccgtcgtc acgagaagac catgcgcccg ccggctccgg
      961 cttcccatgg ccaaaaggcc cctcgggcag ggccgagtgt caggtaaccg ccggacacgg
     1021 ccggcccacg ctcaggggcg ctggcggaac tgccggatac gacgctggct gcggtgcatc
     1081 ttgccgccgg ccctcgggat gatgctgatc gaaccgtcgg gctccaggat cgcggcgctc
     1141 acgtccgcga cgtcggcgat cccgtgctcg cgcgccgaca tctcgatgtc ctccagggag
     1201 acgccttctc gctccatgtc ctcgaggagc ggcttgccgt cctggagcag gagcgtcggc
     1261 tcctcgacga cgaagcggga gaagaacggg agccggcgtc cgaaccgatc gagggccacg
     1321 ttcagcgcga ccagcgtggc cgcggcgacg agtccaccgg tgagcgaaac gtcgccgccg
     1381 ttcagcgagt tctgcacggc gttggcgatg acgaggatca ccacgagatc gggcacgctc
     1441 atctgcccga gctcgcgctt gccggcggcc cggagcagcg cgaacacggc gacgtagacg
     1501 acgaccgtgc ggagcacgat ctcccacacc ggcacggacg gcacgatcat gcccacgccg
     1561 acatgatgcg cttcgtcacg cctcggtccg cgtgccggct acgtcaggat cgcgcgccga
     1621 ccggctgcgc tcggctgggt cccgcggccg cggcgtcggc cttcaccgga tgcgcgatga
     1681 gcaccgagca cggcgcgtgg aagagcacgc cccgcgcgac gctgccgatc acgagccggc
     1741 gcaggccggt ctgcccgcgg cttccgacga cgatgcagtc ggcgtcgcgc tccttcgccg
     1801 cggcgatgag ctcgtaggcc gcgtcgccct cgcgctgctc ggaggtcgcc gttgtcccga
     1861 ggagctcgag tgcctgcgct cgctcgctcg cgaacgccgc gtgcgacgcg cgcagcctcg
     1921 gcatggactc ctcgtacgcg cggtaggcct cgaccgcggc gctcgtgccg gtcgggtcgg
     1981 cgagcaggat cgggaacggc acgtccacca cgctgaccac gtgcacgggg agcttcgcca
     2041 gtggcggaag tccggcgatg acgcgcgcgc cgatcgccga tgtggtggtg ccgtcctcgg
     2101 cgaggaggat gcgttcgagc gtggaccggc gggcgacgag gaccgggcac ggcgcgcggt
     2161 cgacgagctc ggccgagacc gagcccagca cgctggcggc gatgccgcca cgtccacggc
     2221 tgcccacgac gagcaggtcg gcggcgaacg ccgcgcactc gtcgatgatg acgtcggcgg
     2281 cacgcccgta tcccacggtg ctcgtgacct ccacgccgtc acgctcgagc gacttcgccg
     2341 ccgcgcgtac ctgcgcgcgc gcctccgcct cagcggcgtc ggtgatcgtc agcagcgccg
     2401 acggcgcgaa cagggcgacg gtcgtgatcg gctcgatcgc atggacgaca cggacctgtg
     2461 aattggctgg gagcgccatg cccttgacga gcgtgtgtgc catctcggcg ctcgaggaga
     2521 agtcggtcgc gaggaggatc cgcatacggc acctccctgg tgtgcgtcca tggtcggtcg
     2581 gccggccccg cgcggcgtga ggccaacggc gcgagacagc cgtccaaagg gccctgtcgg
     2641 cggtccgcct acacacgtag cgtgcgctca gcaactgaga gacctaggtc accaccgcag
     2701 tggtgatgag aggagtgtcg tatgggtccg ctcgcattca tcagcctggt cgtcatcggg
     2761 ctcgtcatcg gattcgcggt gcagctgctc ggcaagagca agatccggta cgagtggctc
     2821 atcgtcgccg tcgccatcac cttcggtggc tacttcgcga gcgagagctt ccccggcagc
     2881 accgtgttcg aggacatcaa ggactggggg ccggcgttcg acggcctgca cgtgatcccc
     2941 gccgcgatcg gcgcgatcat ccttggtgtc gtcgccgacc tcggcatgcg caccagcccc
     3001 gaaacgcagc ccggctactg acccgtccag cgacaacgcc ggccgccgcg ggatcttgcc
     3061 cacggcggcc gttgccgttc agcagggtcg ggcagccgcc acgtccacag gtgacgagcg
     3121 gccgggccgg ccgcgccgtt cggcactccc tccccggtcg cgggcggacg agcatcaccc
     3181 cgccatgagc acctcggtcg cggcgccgga gatcctcgtt cgcgtcgagc tgacctgctt
     3241 ctactgcggc cacggctacg gggagttcaa ggtccggacc accgggcggc cgacctaccg
     3301 cgacctgcgc cgcgcgatcg aagaggccgc gccggcgaat ccgcccgcct gggacgaacg
     3361 gggcgagccg cgctgtccgc gctgcggtgg aaagctcttc gtcgaggaga gcgaccgccg
     3421 tcggtcgccc gtgcgcggct aggagctgac ctcgcgcagc gcgcggcggg acgcctgccg
     3481 gcgcatgcgg atgctcgttc ccatcaccgc catgaacgag acgaccgcga gcgcgatgcc
     3541 gatcgcgccg gcatcccgta cgtcgccgcg gcccagcgcg tcgccgatca cgcggagcgc
     3601 gaccgagacc tcgaggatcg cgagcggagc gtacagggca cgccggtacg cgatcgccgt
     3661 gccgatcacc gcggggacca cgatcgggcc gtgcgcgaag atcatcgaca tcacgaaacc
     3721 ggcgaagagc gcgtggacca tcgcgtcgta gcgcaagccg ctcgggatgc cgtcgtacgt
     3781 gatcatgaag aatgccgcca cggcgagcca ggcgtaggcg agcggcatcg cggtcgcgat
     3841 gaagcggggc agccccgaca tcctcgcggc ggccggcgga cggtcacgca gcgcgagcca
     3901 cagacccagc acgagcgcgc cggcgcccgc gatccgcagg ccgatggcga gatcgacgag
     3961 cgtgagcatc gtgccggccg cgaaggtggt ggccgcgagg acgaggatcg cgctcgcggt
     4021 cccggaggga cgtagcgcgc gggcgagctc catgcgctcg gccgcgatgg tgaggatgag
     4081 gaaggccgac caccacggca tcgagcggaa ggcggcgctc ccctgccacc acagcaccgc
     4141 accgaccagc cacgcgacgg cgcccgcgct catcaccacg atcgccgtac ccgggctcga
     4201 cggtgcaccg atcaccgtgc cggccaggaa cgcgagcgcg gcgcccgcga cgagcaggcc
     4261 ggcggtccct ccgcccgcgc cgacgatcag tgcgacggcg gcgctcgccg agaggatcgg
     4321 cgcggcgtag ctccaccgac ggcgcatgac gatcgcgcgc tccagcccga tgagcgtgcc
     4381 gatgaagctc gagaccatga gcgccccgtg ataggtcgcg acgtcggtcg gcgccgccgg
     4441 cgcgaggccg agccgcgaga gaccgcccca ccccgccgcg atcagcgcga gcgcggcgag
     4501 gacggcgacc gggacgcgcg aggtcacgcg gtcacgacgt cgtggccttg agtcttgccg
     4561 ccgcggcctc catgcgccgg atgagatccc tgccctcatc catcgtcaga cgatcatcga
     4621 ggagcgggac gtagatctcc tcctccttcg cgaaatgcgt ccgcacgagg gcgtggaggc
     4681 cgtagagcac acgacgcagc gcattctgac gctcgccgtc cggctctccg tggaacgacc
     4741 gcagcgtggc aagctcggag atgagcgcga cgacctcgac gtggtcccgg ctcatggtcg
     4801 cggtcgcccg cggaccgccg agcaaccgcg cgacctcggg atacagcacc tcgtcctcgg
     4861 ccttcgcgtg cggaacgagc tgatgcgcga ggaacgtgta gctctcatcg agcttcgcgc
     4921 ggagcgcggc tccgtcggtg cgtccgacct cgtcggcgac ggcagacagg cgttcgatgt
     4981 tgggaaagag ctcgcgatgc tcctcgcgca gtggggccat cgcgccggcg gtcacggctc
     5041 cacctcactc ttgcgctcga cctggatggt gaagtccgcg gccagcgcgg cgatcgcgcc
     5101 gatggccgcc cacacgggaa cgagcgccac gcccacgaca ccgaaggtga gcggtgcctc
     5161 gatgatggtg gtgccttgcg cgtccttcac gacgacgcgg cggacgttgc cctcgtggat
     5221 gagctgctcg atcttgccga tgaccttgtc gacgttctcg cgatcgagct tgaatgtgtc
     5281 gatcagcgtg tccttgatct gcgtggccat cttcggccct ccttgtggat ctatggctgc
     5341 tctcggcccg gcaccgcgta gcgcgcgggc gaatgctcga aacgcttgcg gcaatcggga
     5401 ccgcagaacg tgtactcgcg gccctcgtaa tcgatcgcga gggagtgctg cgccgcatcc
     5461 tcgagctgga ccgtggcccc gcagacggga tcgatctcga tctctgactc ggattcgatg
     5521 cgaatctcgt cactcatggc cattccctcc attcgcagga cggaccgacg ttagggtcgc
     5581 gtgcccgaca gggcgagggt ccaaggaccg tcccgtccat ggccgtgcgg cacttcgcgc
     5641 gccagggccg gcggcgcagg ctccatcgca tgggaacacc caaggaacgg ctcgaagcca
     5701 tgctcgccga gctccgcgcg gaggtcggtg aggacatcgc gaacctcgcg cgcgacatcg
     5761 actcccgtgg cgaggacacc acgccatcgc agcatcccgc cgacgtcgcg tcggacctgt
     5821 acgcccgcga ggagctcgtc gtgcacgagc tcgcgctcgc gcacgaggtg gaggagatcg
     5881 agggcgcact gcgcaggatc gccgcgggaa cgtacggtcg gtgcgaggag tgcggcggca
     5941 cggtggatcc ggctcggctc gaggcgcggc cgcaggcggc gctctgcatc acatgccagc
     6001 gccggcgtga ggaccgacga ggacgttcgc tcgcgcgtgc gtgacgacgc gctcggccgt
     6061 gcttccgaag aacagctgac gcgggccgga tagaccgcgc gtgccgatga cgatgagatc
     6121 ggcaccgatg tccctcgcgc gccgccggat cgtttcgccc acgtggccgc gctcgtggcg
     6181 cgtggtcacc gccagccccg gcgggagcag cgtccgctgc gcgtccagca tcgcg
//
LOCUS       DASTZR010000118         3021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_868850, whole genome shotgun sequence.
ACCESSION   DASTZR010000118 DASTZR010000000
VERSION     DASTZR010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868850"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VFV20_02780"
     CDS             <1..378
                     /locus_tag="VFV20_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU5287303.1"
                     /translation="YGMPGVRVDGLDVRAVYEAAGELIARARRGEGPSLLVAEAYRHE
                     GHYYGDPRKYQTKDQIEGWKAKYDPIVDARKWIVDGKVATAAELDAIDDAVVEEAKKA
                     VAFAEASPIATPIKLPGDVYASH"
     gene            365..1372
                     /locus_tag="VFV20_02785"
     CDS             365..1372
                     /locus_tag="VFV20_02785"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU5287304.1"
                     /translation="MQATETRAPAATREMFYNEALGDALRLEMERDERVIVMGEDIAE
                     HGGAFQVTAGLLDTFGPTRIRQTPISELGIVGLAVGAALTGLRPVAELMYIDFAGLAM
                     DQLVNQAAQNRFMFGGQARVPMVMRTQGGSGRGNAAQHSKSLEAWFAHVAGLKVVMPA
                     TPADAKGLLTAAIRDDDPVVFLEHKLLYRTKGEVPTGEHVVPLGNADVKKTGDALTIV
                     SWSRHVLLALEAAKQLSAEGIEAEVVDLRSLVPLDRDAVLSSVRKTHRVLVVHEAIKR
                     AGFGAEIAAMIAEEAFDDLDAPPRRLAGMETPIPYAQHLEKTVIPQVEDIVKAAKELV
                     A"
     gene            1369..2565
                     /locus_tag="VFV20_02790"
     CDS             1369..2565
                     /locus_tag="VFV20_02790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEU5287305.1"
                     /translation="MSVTRVILPKLGLTMDEGRLIAWHKQEGDRVEKGEILFEVETDK
                     ANMEIESPASGYVRHLLVEADATVPVTTLIALISDTPDEDIPDAMHVARGTPEVAGAT
                     PEAAEASPSLAQGVPSLASPAAGDGERVRSSPAARKRAQELGVDINRVRGSGPGGRIN
                     IEDVEAAAVAKAPASAVAAADAAMGEHREPLTRMRRAVADAMTRSVRDAPQFSISRDV
                     DMTNANARRKAVGVSYTDVIVAAAANALRAHPRLRSTFDGDAIVTREATDVGIAVALD
                     AGLIVPVVRDAGNKPLRTLRDEREALEAAVASGHARADAFGGAALTVSNLGTFGVDRF
                     TAIVNPPEASILAVGRVTDRVVVVDGVPAVRPMCSLTLTVDHRVADGADAARYLADVA
                     KELESA"
     gene            2562..>3021
                     /locus_tag="VFV20_02795"
     CDS             2562..>3021
                     /locus_tag="VFV20_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5287306.1"
                     /translation="MTDGVHLAASHWKPRFIANGIDVNDFESVLDTASEWRDWAPAWQ
                     RIGDMHRALGEQAERAGHRVTAAAAFQRAAWCYHLGKFLWFEDVALHHALSEATVAAY
                     ARALPDIDPPGTRIEAPFEGAVIPGILRKPAGGGRPPLVILVPGLDSVKEE"
BASE COUNT          467 a         1041 c         1113 g          400 t
ORIGIN      
        1 tacggcatgc cgggcgtgcg cgtcgacggg ctcgacgtgc gcgccgtgta cgaggccgcc
       61 ggggagctca tcgctcgcgc acggcgcggc gaagggccgt cgctgctggt cgccgaggcg
      121 taccgtcacg agggccacta ctacggcgat ccgcggaagt accagacgaa ggatcagatc
      181 gagggctgga aggcgaagta cgaccccatc gtcgacgcac gcaagtggat cgtcgacggc
      241 aaggtcgcga cggccgctga gctcgacgcg atagacgatg ctgtggtcga ggaggcgaag
      301 aaggccgtcg cgttcgcgga ggcatcgccc atcgccacgc ctatcaagct gccgggggat
      361 gtctatgcaa gccactgaga cgcgggcgcc ggccgcgacg cgcgagatgt tctacaacga
      421 ggcgctgggc gacgcgctgc gcctcgagat ggagcgcgac gagcgcgtca tcgtgatggg
      481 cgaggacatc gccgagcacg gcggcgcgtt ccaggtgacg gcggggctgc tcgacacgtt
      541 cggacccaca cgcatccgcc agacgccgat cagcgagctc ggcatcgtcg gcctcgccgt
      601 gggcgcggcg ctcacggggc tgcgcccggt ggccgagctc atgtacatcg acttcgcggg
      661 cctcgcgatg gaccaactcg tcaaccaggc cgcgcagaac cgcttcatgt tcggcggaca
      721 ggcacgcgtg ccgatggtga tgcgcacgca gggcggctcc ggccgcggca acgcggcaca
      781 gcactccaag tcgctcgagg cgtggttcgc gcacgtggcc ggcctcaagg tggtcatgcc
      841 cgccacgccg gcggacgcga agggcctgct caccgccgcc atccgcgacg acgacccggt
      901 cgtcttcctg gagcacaagc tcctctaccg gaccaagggc gaggtcccca cgggcgagca
      961 cgtcgtgccg ctcggcaatg cggacgtcaa gaagaccggc gacgcgctca cgatcgtgag
     1021 ctggtcgcgg cacgtcctcc tcgcgctcga ggcggcgaaa cagctgtccg ccgaggggat
     1081 cgaggcggag gtcgtggacc tgcgcagcct cgtgccgctc gacagggacg cggtgctctc
     1141 gtcggtgcgg aagacgcatc gcgtgctggt ggtgcacgag gcgatcaagc gcgcgggctt
     1201 cggcgccgag atcgcggcga tgatcgcgga ggaggcgttc gacgacctcg acgcgccacc
     1261 gcgccgcctg gccggcatgg agacgccgat cccgtacgcg cagcatctcg agaagaccgt
     1321 gatcccccag gtcgaggaca tcgtgaaggc ggcgaaggag cttgtcgcgt gagcgtgacg
     1381 cgcgtgatcc tgccgaagct cgggctcacg atggacgagg gtcgtctcat cgcgtggcac
     1441 aagcaggaag gcgaccgcgt cgagaagggg gagatcctct tcgaggtcga gacggacaag
     1501 gccaacatgg agatcgagtc gccggcctcc gggtacgtgc ggcatctcct ggtggaggcc
     1561 gatgccaccg tcccggtgac gacgctgatc gcgctcatca gcgacacgcc cgacgaggac
     1621 atcccggacg cgatgcacgt ggcgaggggc acgcccgagg tcgcgggtgc cacacccgag
     1681 gccgcggagg ccagcccatc gctcgctcag ggcgtgccgt cactggcgtc gcccgcggcc
     1741 ggggatggcg agagagtgcg ttcctctccc gcggcgcgca agcgcgcgca ggagctcggc
     1801 gtcgacatca accgcgtgcg cggctcgggt ccgggtggcc gcatcaacat cgaggacgtc
     1861 gaagcggccg cggtcgcgaa ggcgcccgcc tcggccgtgg cggcggcgga cgcggcgatg
     1921 ggcgagcatc gcgagccgct cacgaggatg cggcgcgcgg tcgccgacgc gatgacacgc
     1981 tccgtccgcg acgcgccgca gttctcgatc tcgcgcgacg tcgacatgac gaacgcgaac
     2041 gcccgccgca aggcggtcgg cgtctcctat accgacgtga tcgtcgcggc cgcggcgaac
     2101 gcgctccggg cgcatccgcg tctgcgctcg acgttcgacg gcgatgcgat cgtcacccgc
     2161 gaggcgacgg acgtgggcat cgcggtggcg ctcgacgccg gcctcatcgt gccggtcgtc
     2221 cgcgatgccg ggaacaagcc gctgcggacg ctccgcgacg agcgtgaggc gctcgaggcc
     2281 gcggtggcgt ccgggcacgc ccgcgccgac gcgttcgggg gcgcggcgct caccgtctcg
     2341 aacctcggca cgttcggcgt ggaccgcttc accgcgatcg tgaacccacc ggaagcgtcg
     2401 atcctcgcgg tcggccgcgt caccgaccgc gtcgtcgtgg tggacggcgt accagcggtg
     2461 cggccgatgt gcagtctcac gctcaccgtc gatcatcgcg tcgcggatgg cgcggacgcg
     2521 gcccgctacc tcgcggatgt cgcgaaggag ctggagtcgg catgacggac ggcgtccacc
     2581 tcgccgcctc gcactggaag ccacgcttca tcgcgaacgg catcgacgtc aacgacttcg
     2641 agtcggtgct cgacacggct tccgagtggc gcgactgggc gccggcgtgg cagcggatcg
     2701 gcgacatgca tcgcgccctc ggcgagcagg ccgagcgcgc cgggcaccgc gtgaccgcgg
     2761 ccgccgcgtt ccagcgggcg gcctggtgct accacctcgg gaagttcctg tggttcgagg
     2821 acgtcgcgct gcaccacgcg ctcagcgagg cgacggtcgc cgcgtacgcg cgcgccctgc
     2881 cggatatcga tccgcccggc acgcgcatcg aggcgccgtt cgagggtgcc gtgatccccg
     2941 ggatcctgcg caaacccgcg ggtgggggac gtccgccgct cgtcatcctc gtccccggcc
     3001 tcgactccgt gaaggaagag c
//
LOCUS       DASTZR010000119         2989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_451009, whole genome shotgun sequence.
ACCESSION   DASTZR010000119 DASTZR010000000
VERSION     DASTZR010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451009"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VFV20_02800"
     CDS             complement(<1..483)
                     /locus_tag="VFV20_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287307.1"
                     /translation="MSGGPAGGGGPHLALFDHHIHTDRSDGTVSLAARAETVRVRPHG
                     VSDHFPWGERLRTDDDVLRYVDDASRLGLRVGIEYDLGVAPELRQTTRDALHYVIGAL
                     HQLELADGEWIRFDEAGAYLKGRIHTFSQVERFLDAGLQERIRERTLAVVRAGIERDG
                     I"
     gene            complement(480..1583)
                     /locus_tag="VFV20_02805"
     CDS             complement(480..1583)
                     /locus_tag="VFV20_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287308.1"
                     /translation="MTTLLLFFGRGGPSDVEHRLDAIKIAIGASVLRRAAEAGFARRI
                     AVTADGEGGRAFAAAGAEVVPPRTSPFHFGEEVAALVSERKLERVCTIGAGAGALFGV
                     PELRGLREELETSEALVLSNNYYSADLIAFTPASALNVIELPATDNPLPRRLHQQGGL
                     SSRQLDRSAATLLDVDTPADAAVLKRHPRCPPELRAVDTWDAELGDRIDALMRLVTTP
                     ERELVVAGRVGAPVWSYLESQTACRVRMLAEERGMQSAGRDASGDARTALGFLYEEIG
                     PERFFARMGELGDGFLFDTRVLFAHLGWHAPGAERFASDLFRAGDISEPRLAAFTAAA
                     AAAPYPLLLGGQTLVSGVLWTLVEAAWAGHPDA"
     gene            complement(1580..>2989)
                     /locus_tag="VFV20_02810"
     CDS             complement(1580..>2989)
                     /locus_tag="VFV20_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287309.1"
                     /translation="IWSPDVIPNALLPFTLLREGDVDYDEFVFRPNQTPSTAGDAVAK
                     IDSEAYFFRACGASTATERPRAPRSKGGPPAPGPNDHVCSVFPPGMGLLALPFFLPFV
                     ATGAEALDLGMLIRVGHVAAASYEVLATLLLWAVLRRLVGERSALGLVLLYFLGTSAR
                     TVSSQALWQHPGAHLALALALWLTIREGAPARPRELAAGLALGLGGLVRQTTALASLG
                     LTRWGDGIHLTGGRALDVALLGGLAVGVVPLLLWNWLAYGAPLEQGYGAKPFDTPVLE
                     GLAGLLISPSRGLFVYEPWAILALGSMVIARFAKTTRGELSRRIADLILAWLVFVALY
                     ATYAEWWGGRVFGPRFLDDLAPMLVVGLAWGVRGGWFAFGFMRALLVLSTAWSLLLFN
                     AAAIVYDQNTWDLVPVNVNDDPSRLFSWSDPQWLAVLQQLPSGGVRVVAAVLLSLLAL
                     AFLARVEGLIGRRVLASPR"
BASE COUNT          486 a         1068 c         1102 g          333 t
ORIGIN      
        1 gatgccgtcc cgctcgatgc ccgcacgcac gaccgccagc gtccgctcgc ggatgcgctc
       61 ctgcaggccg gcgtcgagga agcgctcgac ctgcgagaac gtgtggatgc ggcccttgag
      121 gtacgcaccg gcctcgtcga agcggatcca ctcgccatcc gcgagctcga gctgatgcag
      181 cgcgccgatc acgtagtgca gcgcgtcgcg cgtggtctgg cgaagctcgg gcgcgacgcc
      241 gaggtcgtac tcgatgccca cccgcaggcc gagccgcgag gcgtcgtcga cgtagcgcag
      301 gacgtcgtcg tcggtccgca gccgctcgcc ccacgggaag tgatcggaca cgccgtgcgg
      361 ccgcacccgc acggtctcgg cgcgtgccgc gaggctcacg gtgccgtccg atcgatcggt
      421 gtggatgtgg tggtcgaaca gcgcaaggtg gggtccgccg ccccccgccg gacccccgct
      481 cacgcgtcgg ggtggccggc ccacgcggcc tcgacgagcg tccacagcac gccggacacg
      541 agcgtctgac cgccgaggag cagcgggtac ggcgccgcgg cggcggcggc ggtgaacgcc
      601 gcgaggcgcg gctcgctgat gtcgcccgca cggaacagat cggaggcgaa gcgctcggcg
      661 ccgggcgcat gccagcccag atgcgcgaac agcacgcgcg tgtcgaagag gaagccatcc
      721 ccgagctcgc ccatgcgcgc gaagaaccgc tccgggccga tctcctcgta caggaacccg
      781 agggccgttc gcgcatcgcc gctggcgtcg cgtccggccg actgcatccc gcgctcctcc
      841 gcgagcatgc gcacgcggca ggcggtctgg gattcgaggt acgaccagac gggcgcgccc
      901 acgcgcccgg cgacgacgag ctcccgctcg ggcgtggtca cgagccgcat gagcgcgtcg
      961 atgcggtcac cgagctcggc gtcccaggtg tccaccgcgc gcagctcggg cgggcagcgc
     1021 ggatgacgct tgagcaccgc cgcatccgcg ggcgtgtcca cgtcgagcag cgtcgcggcg
     1081 gagcggtcga gctggcgcga cgagagcccg ccctgctgat ggagacgccg cggcagcggg
     1141 ttgtccgtcg cgggaagctc gatcacgttc agcgcggacg ccggcgtgaa cgcgatgagg
     1201 tccgccgagt agtagttgtt cgagagcacg agcgcctcgc tcgtctccag ctcctcccgc
     1261 agtccacgca gctccggcac gccgaagagc gcgccggcgc ccgcgccgat ggtgcagacg
     1321 cgttccagct tccgctcgga cacgagggcc gcgacctcct cgccgaagtg gaacggcgac
     1381 gtgcgcggcg gcacgacctc ggcgcccgcc gcggcgaagg cgcggccacc ctcgccgtcc
     1441 gcggtgacgg cgatccggcg cgcgaagccg gcctcggcgg cgcgacgcag cacggacgcg
     1501 ccgatggcga tcttgatcgc gtcgagtcga tgctcgacat cggagggacc gccacggccg
     1561 aagaagagca ggagcgtggt caccgcggcg acgctaacac ccgacgaccg atgagtccct
     1621 ccacgcgcgc gaggaaggcg agcgcgagca gtgagagcag gacggccgcg accactcgca
     1681 caccgccgct cggcagctgt tgcagcacgg ccagccactg cgggtcgctc caggagaaga
     1741 gccgcgacgg gtcgtcgttc acgttcaccg gcacgaggtc ccaggtgttc tggtcgtaca
     1801 cgatcgcggc ggcgttgaag aggagcagcg accacgcggt cgacagcacg aggagcgcgc
     1861 gcatgaagcc gaaggcgaac cacccaccgc gcacgcccca cgcgagcccg acgacgagca
     1921 tgggcgcgag atcgtcgagg aagcggggac cgaacacgcg gccgccccac cactccgcgt
     1981 acgtcgcgta gagggcgacg aagacgagcc aggcgaggat caggtccgcg atgcggcggg
     2041 agagctcgcc gcgtgtcgtc ttcgcgaacc gagcgatcac catgctgccc agcgcgagga
     2101 tcgcccacgg ctcgtacacg aagagaccgc ggctcggcga gatgaggagg cccgcgaggc
     2161 cttccagcac cggcgtgtcg aacggcttcg cgccgtagcc ctgctcgagg ggcgcgccgt
     2221 acgcgagcca gttccagagg agcagcggca cgactcccac cgcgagcccg cccaggagcg
     2281 cgacgtcgag ggcgcggccg ccggtgaggt ggatgccgtc accccagcgt gtcagtccga
     2341 ggctggcgag cgccgtggtc tgccggacga ggccgccgag acccaacgcg agacccgcgg
     2401 cgagctcacg cggccgcgcc ggcgctccct cgcggatggt gagccaaagc gcgagcgcga
     2461 gcgcgagatg cgcgcccggg tgctgccaca gtgcctgcga cgagaccgtg cgcgcgctcg
     2521 tgccgaggaa gtacagcagc acgagcccga gcgccgaccg ttcgccgacg agccggcgca
     2581 gcacggccca caggaggagc gttgccagga cctcgtagga cgcggccgcg acgtgaccga
     2641 cgcggatgag catcccgagg tcgagcgcct cggcgccggt ggcgacgaac ggaaggaaga
     2701 acggcagcgc gagcaggccc atccccggtg ggaacaccga gcacacgtgg tcattcggtc
     2761 ccggcgcggg gggaccgccc ttgctgcgtg gcgcgcgcgg gcgctccgtc gcggtcgacg
     2821 ccccgcaggc acggaagaag tacgcctcgg agtcgatctt cgcgaccgcg tcgccggcgg
     2881 tcgacggcgt ctggttgggc cggaagacga actcgtcgta atcgacgtcc ccctcacgca
     2941 gcagcgtgaa cggcagcagc gcgttcggaa tgacgtctgg cgaccagat
//
LOCUS       DASTZR010000120         3557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_371075, whole genome shotgun sequence.
ACCESSION   DASTZR010000120 DASTZR010000000
VERSION     DASTZR010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371075"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..150
                     /locus_tag="VFV20_02815"
     CDS             <1..150
                     /locus_tag="VFV20_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018350529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEU5287310.1"
                     /translation="NREIAEKLVLSPETVKSHVAAILEKLNVSDRTQAAIYAVRNGLV
                     ESPSA"
     gene            complement(158..1399)
                     /locus_tag="VFV20_02820"
     CDS             complement(158..1399)
                     /locus_tag="VFV20_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287311.1"
                     /translation="MLARARANSGALFLLVATTVANVLAYGYQVVQARLLPPGEYAAL
                     TALFGILILESVSSQVIQSATATLTARYRAREEHEALHAFVRRWSVRIGGASAALGVA
                     VVLLSPLVASTLSLPPVSVALVGVALFLALVFTFVVGLLQGLARFVWMGVALIAQAGS
                     RLVIGVALVLLGLGVDGAFTGATAAIGVSLAVAAIPVLGLMRAARRTTAVVELGAAET
                     RFFVLSAVVLLAYAALTNIDAVLARSLLAPDEAGAYAGAITMGKIVLFAPIAVGYILL
                     ERTASAHARGDDTDRTLFLALGFVLVTSGLVAVAYITVPAFLVPIVVGSQYPETPRIV
                     GVYGLAALSNALLNLWIAYFIGRGEMRVGLLLALAVAAEIVLLFTTVSDAVSMARVVL
                     EVALATQAAAIVTYVVKRRAR"
     gene            1526..1654
                     /locus_tag="VFV20_02825"
     CDS             1526..1654
                     /locus_tag="VFV20_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287312.1"
                     /translation="MELLLFIVLATLLAIAAPVRGVDSRDTLRPAEITGSHAPLTA"
     gene            complement(1715..2770)
                     /locus_tag="VFV20_02830"
     CDS             complement(1715..2770)
                     /locus_tag="VFV20_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEU5287313.1"
                     /translation="MAVQITPDMTVASVVDRWPDAARLFARYGLSCASCAISKSETIA
                     QAAAGHGAGRVKMDELMGDLNRYAETGQLPEGLPAAGAAPSLGGKGVPRGMAEQKGIK
                     HVIAVMSGKGGVGKSLVAGLLAVGLKRRGFSVGVMDGDITGPSIPRMFGVKEKPVSPD
                     GKTLQPPKSRGGIPIMSMNLILPSEEEAVIWRGPMVAGAIRQFFTDVQWGELDYLIVD
                     LPPGTSDAPLTVMQALPVDGVVLVTTPQALATMIVTKAVRLVKQLGGDILGVVENMSY
                     VKGPDGSRMEVFGPSQGLKLVIAANAPLIANIPIDPEIAKDADSGDIESYTSPETDDL
                     VENFLKAAPLKEPVAAR"
     gene            2864..3550
                     /locus_tag="VFV20_02835"
     CDS             2864..3550
                     /locus_tag="VFV20_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287314.1"
                     /translation="MQQSLSDRRSDPRRGTLVRVGLVLTATGIMSTALDQRALVGGRF
                     GVSTAIAFALALALLFIATFRRPVPGAPWIALAATAIVLLLAAAQLDATVGMAAYVLV
                     AVVAVARTAPHLRALTAAAFALWTPSLWSFGAASGAALSAPLRLAAMAALAYTVFVLV
                     DPRRVHPSDRLRRAGHGMLAIAVVSAAAARTLVVAPSFSIPDVIAIAAVVSARRCEAV
                     RATATRATSR"
BASE COUNT          515 a         1270 c         1275 g          497 t
ORIGIN      
        1 aatcgcgaga tcgccgagaa gctcgtgctc tcgcccgaga ccgtgaagag ccacgtcgcg
       61 gccatcctcg agaagctcaa cgtctccgac cgtacccagg ccgccatcta cgcggtacgg
      121 aacggcctgg tggagagccc ctccgcctga tcccggccta gcgcgcgcgt cgcttcacca
      181 cgtaggtcac gatcgccgcc gcctgcgtcg cgagcgcgac ctcgaggacg acacgcgcca
      241 tcgacaccgc gtcgctcaca gtcgtgaaca gcaggacgat ctcggcggcg accgcgagcg
      301 cgagcaggag gccgacgcgc atctcgccgc ggccgatgaa gtacgcgatc cagaggttga
      361 gcagcgcgtt cgacagcgcc gcgagtccgt acacgccgac gatccggggc gtctccggat
      421 actgggagcc gacgacgatc ggcacgagga aggcggggac ggtgatgtac gcgaccgcga
      481 ccaggccgct cgtcacgagc acgaagccga gcgcgaggaa cagcgtccgg tcggtgtcgt
      541 cgccacgcgc gtgggcgctg gcggtgcgct cgaggaggat gtagcccacc gcgatcggag
      601 cgaagagcac gatcttcccc atcgtgatcg cccccgcgta cgcacccgcc tcatcgggcg
      661 cgagcagcga gcgcgcgagg accgcgtcga tgttggtgag cgccgcgtac gcgagcagca
      721 ccacggccga aaggacgaag aagcgcgttt cagccgcacc gagctcgacc acggccgtcg
      781 tgcgacgggc cgcgcgcatg agccccagca ccggtatcgc ggccacggcc aggctcacgc
      841 cgatcgcggc ggtcgcgccg gtgaacgcac cgtcgacccc gaggccgagg agcacgagcg
      901 cgacaccgat caccagacgg ctccccgcct gcgcgatgag cgcgacgccc atccagacga
      961 agcgcgcgag gccctgcagg agacccacca cgaacgtgaa gacgagcgcg aggaacaggg
     1021 ccacgccgac gagcgcgacg gagacggggg gcagggacag cgtgctggcg acgagcggcg
     1081 agaggaggac caccgcgaca ccgagcgcgg cggacgctcc cccgatgcgc accgaccacc
     1141 ggcggacgaa tgcgtgcagc gcctcgtgct cttcgcgcgc gcggtagcgg gcggtcagcg
     1201 tcgccgtcgc ggattggatg acctggctcg agacggactc gaggatgagg atcccgaaga
     1261 gcgcggtgag cgcggcgtac tccccgggcg gcaggagccg cgcctggacg acctggtagc
     1321 cgtacgcgag cacgttcgcg acggtcgtcg cgacgaggag gaacagcgcg ccggagttcg
     1381 cgcgggcgcg agcgagcatc cgtgaatact gccgtcaggt ggcgcgggct gctggcgtcg
     1441 tcccgccggc gacgcgccgg atggccggcg ggtggaggcc gctcgaccct atcgcgggcg
     1501 tggcgccggg gcggatgctt gccgcatgga gctcctgctg ttcatcgtcc tcgcgaccct
     1561 gctcgcgatc gcggcacctg ttcgcggcgt cgactcgcgc gacaccctgc gccccgcgga
     1621 gatcaccgga agccacgcac cgctgaccgc gtagcgccac cctgcggcgc cgtcctcggc
     1681 gcaccacacg tggtgcccgc ccgcacgacg cgcgctaccg cgcggcgacg ggctccttga
     1741 gcggcgcggc cttgaggaag ttctcgacga ggtcgtccgt ctcgggcgag gtgtacgact
     1801 cgatatcgcc ggagtccgcg tccttcgcga tctccggatc gatcgggatg ttcgcgatga
     1861 gcggtgcgtt cgcggcgatg acgagcttca ggccctgcga cgggccgaag acctccatgc
     1921 gtgagccgtc ggggcccttc acgtacgaca tgttctcgac gacgccgagg atgtcgccgc
     1981 cgagctgctt cacgagcctc acggccttgg tgacgatcat cgtcgcgagc gcctgcggcg
     2041 tggtcacgag cacgacgccg tcgacgggca gtgcctgcat caccgtgagc ggcgcgtcgc
     2101 tcgtccccgg cggcaggtcg acgatgaggt agtcgagctc gccccactgc acgtcggtga
     2161 agaactgccg gatggcgccg gccaccatgg gtccgcgcca gatgaccgcc tcctcctcgc
     2221 tcgggaggat gaggttcatc gacatgatcg ggatcccgcc gcgcgacttc ggcggctgga
     2281 gcgtcttccc gtcgggcgag accggcttct ccttcacgcc gaacatgcgc gggatggacg
     2341 gtccggtgat gtcgccgtcc atgacgccga cgctgaatcc gcggcgcttc agtcccacgg
     2401 cgagcaggcc ggcgacgagc gacttgccca cgccgccctt gccggacatg accgcgatga
     2461 cgtgcttgat gcccttctgt tcggccatgc cgcgcggcac gcccttgccc ccgaggctgg
     2521 gcgcggcgcc ggcagccggc aggccctccg ggagctgtcc cgtctccgcg tagcggttga
     2581 ggtcgcccat gagctcgtcc atcttcacgc ggcccgcgcc gtgtccggcc gccgcctgcg
     2641 cgatcgtctc gctcttgctg atggcgcacg acgcgcacga caggccgtag cgcgcgaaga
     2701 ggcgcgccgc atccggccag cggtcgacga ctgatgcgac ggtcatgtcg ggagtgatct
     2761 ggacggccaa agacgggctc cttccggacg cgtggggctg ggtcaagtgt acggggtcga
     2821 tgcccgcgaa cacggtcgca cgcccgcgcg ctagcctgcc gccgtgcagc aatcgctgag
     2881 cgatcgccgg tcggacccgc gtcgcgggac cctggtccgc gtcggtttgg tgctgaccgc
     2941 gaccgggatc atgagcaccg cgctcgatca gcgcgctctc gtcggcggga gattcggcgt
     3001 gtccaccgcc atcgcgttcg ctctcgcgct cgccctgctg ttcattgcga cgttccgccg
     3061 tccggtcccc ggtgcgccgt ggatcgcgct cgccgcgacg gcgatcgtgc tgctgctggc
     3121 ggcggcgcag ctcgatgcga cggtgggcat ggccgcgtac gtgctcgtcg ccgtcgtcgc
     3181 ggtcgcgcgg acggcaccgc atctgcgcgc gctcaccgcc gcggcgttcg cgctgtggac
     3241 gccgtcgctc tggtccttcg gcgcggcgtc cggcgccgcc ctctccgcgc cgctccggct
     3301 cgcggcgatg gcggcgctcg cgtacacggt gttcgtgctc gtcgatccgc gacgcgtcca
     3361 cccgtcggat cggctgcgtc gcgccggtca cgggatgctg gcgatcgcgg tcgtgtcggc
     3421 ggccgcggcc cgaacgctcg tcgtcgcgcc gagcttctcg atcccggacg tcatcgcgat
     3481 cgccgccgtc gtgagcgcgc ggagatgcga ggccgtccgc gcgaccgcga cgagggcgac
     3541 gagcagatag atggtca
//
LOCUS       DASTZR010000121         5767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_607906, whole genome shotgun sequence.
ACCESSION   DASTZR010000121 DASTZR010000000
VERSION     DASTZR010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5767
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607906"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="VFV20_02840"
     CDS             <1..915
                     /locus_tag="VFV20_02840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03620 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEU5287315.1"
                     /translation="VRDDVRVWRERLGPVGAWTFDAERLSAEDARAYGRELESLGVPS
                     LWLPESLGSKEIFAHASLFLAATSRLVIATGIANIWARDPVAMANGQRALCDAYPGRF
                     LLGLGVSHAPVIPMRSKGARYDRPLERMREYLDAMDKAPYTGKAVETPRVLAALGPKM
                     LKLSAERALGAHPYFVPVEHTSIARAEVGPDALLAVEQAAVLATDPAVARATARRHMK
                     RYLDLDNYANNLRRLGWADADLANGGSDALVDAVVAWGDADAIRQRVEEHRRRGADHV
                     CVQVIRVDVAAPATLEWRALAPVLIPRR"
     gene            complement(1013..1333)
                     /locus_tag="VFV20_02845"
     CDS             complement(1013..1333)
                     /locus_tag="VFV20_02845"
                     /inference="COORDINATES: protein motif:HMM:NF013286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEU5287316.1"
                     /translation="MADLTQRVAAKLAEMSPYIERSGGRVELVDVEDGIARLRVGLTR
                     PGPSRLVASLQLKSGIERSLRNDIPELRGVEAVNLPPYAELGWDQPAFAPVELPVPGD
                     DSAA"
     gene            1378..2139
                     /locus_tag="VFV20_02850"
     CDS             1378..2139
                     /locus_tag="VFV20_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEU5287317.1"
                     /translation="MPPELPPDLRPISGGAGGADVILTSEDATRFRAYLAKANAGDIG
                     VVIAPDVRGLHHFYEELAERFADAGVHAIAFDYFGRTAGQDRRPDDFPFRDHVPLTKP
                     DQIQADTASAIGHLRGATDARRIFVLGFCMGGRVAFNASADQPEISGAIGFYGRLSTR
                     PGEEGTAPADRARRMRAPILGLFGGSDPGIPADEVRAFDAALTAEKVPHHIETYAGAP
                     HSFFDRTYDDHKDACADAWRRALGFMRTGDPAARA"
     gene            2136..2552
                     /locus_tag="VFV20_02855"
     CDS             2136..2552
                     /locus_tag="VFV20_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287318.1"
                     /translation="MIGAVLYLAVGTVVFVAITFLEQVPVLGWLGGVVSIAAWIALSR
                     GLARGMGADPLRSGAAIIWSGVLGAFTGFVGALTAWLAQTGNLFGFTTPPGDRFGAAF
                     GFVGASIGLIYWPVIGALVCVATAFMVAPRRGGDRL"
     gene            complement(2549..4552)
                     /locus_tag="VFV20_02860"
     CDS             complement(2549..4552)
                     /locus_tag="VFV20_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU5287319.1"
                     /translation="MNEIPRLDDFGRDIRYSRWDGTQRLDALEADELLAAMSDELLSG
                     GDLEDAMARLSRWGIPGRMEGMKDLLERLRSAKQRRAQRHDLSTAFDQLKEKLEDVKR
                     LEREGLERRRAAEAPNEQLKRAMERLAQERLAQLDELPEDFGRAVRALKDYEFVEPKA
                     AEKYQELLKELQQQVLGSYFKNMRDSLQNLTPEQLERTRQMIRDLNRALKERMEGGEP
                     DFEAFQRQYPELAGGAKDWDDLLRQLAQGMAAMRSLWQSMSTDMQQQLEELLGAAFND
                     PGLQSAMNDLQDTLSQLMPIESYEHGMRGDEPITLAEALGLMDEMNQLSDMEEVVRGA
                     RESGDLDPLDPDQVERLAGAGARQSLEQLQRMSKLLEEAGLIRKDGERYELTPRGIRK
                     IGQRSLEEIFATLKRDAFGSHRSRVRGRGGDPTDELKTYEFGDPFLLDLPATVRNAVF
                     REGAKVPVKLQPQDFDVYRTELMTQSATAILVDVSRSMLFRGCFLAAKKVTLALDSLI
                     RTTFPKDDLYIIGFSAYAQELKPSELPGLTWNEYVYGTNMQHAFSTARGLLSRSRGKN
                     KQIILITDGEPTAHFEVGDPEPRFNYPPTKRTFEETLREVVRCTREQITINTFMLARG
                     HYLVDFVNQMSKLNTGRAFYVEPERLGEYVLVDYVGHKRRKVS"
     gene            complement(4549..>5767)
                     /locus_tag="VFV20_02865"
     CDS             complement(4549..>5767)
                     /locus_tag="VFV20_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEU5287320.1"
                     /translation="GHDVIFLGERGQAKTRMIRSLVGLLDEWLPIVAGSEINDDPTNP
                     VSAHARNLVDELGDATPIDWVHRDRRFGEKLATPDTSIADLIGEVDPIKVAEGRYLSD
                     ELTIHYGLVPRTHRGIFALNELPDLAERVQVSLLNVMEERDVQIRGYKVRLPLDLFVV
                     ASANPEDYTNRGRIITPLKDRFGSQIRTHYPKEIGIEVGIMEQERNTFVDEGITTIVP
                     GYMKEILAEVSQLARRSPEISQRSGVSVRVSVANYENLVSSALKRALRNGETTVVPRI
                     SDLPAVIASTAGKIELESVGDVTEERVIERLAQRAVLNVFNRTFSLGEFDALLAAFAR
                     GATMHVSSALPSTEYVKQALQIPGMKGAVAKLGAAGNPSTVAAAVEFVLEGLHLNRKL
                     NKERGESRSTFRA"
BASE COUNT          794 a         2019 c         2008 g          946 t
ORIGIN      
        1 gtgagggacg atgtccgcgt ctggcgtgag cggctcgggc cggtgggtgc ctggaccttc
       61 gacgcggagc gcctctccgc cgaggacgcg cgcgcgtacg ggcgcgagct ggagtcgctg
      121 ggcgtcccgt cgctctggct accggagtcg ctcggcagca aggagatctt cgcgcacgcg
      181 tcgctgttcc tcgcggccac gtcgcggctc gtgatcgcga ccggcatcgc gaacatctgg
      241 gcgcgcgacc ccgtggcgat ggcgaacggc cagcgcgcgc tctgcgacgc gtacccgggg
      301 cggttcctgc tcggcctcgg cgtgagccac gcgccggtga tcccgatgcg aagcaagggc
      361 gcgcgctacg accgcccgct ggagcgcatg cgcgagtacc tcgacgcgat ggacaaggcg
      421 ccgtacaccg gcaaagccgt cgaaacgccg cgcgtgctcg ccgcgctcgg accgaagatg
      481 ctgaagctgt cggccgagcg cgctctggga gcgcacccgt acttcgtgcc ggtcgagcac
      541 acctcgatcg cgcgcgcgga ggtcggcccg gatgcgctcc tcgccgtcga gcaggccgcg
      601 gtgcttgcga cggatcccgc cgtcgcccgc gcgacggcac gccggcacat gaagcgctac
      661 ctggacctcg acaactacgc gaacaacctg cggcgcctcg gctgggcgga cgccgacctc
      721 gcgaacggcg gcagcgacgc gctcgtcgat gcggtcgtcg cgtggggcga cgccgacgcg
      781 atccggcaac gggtcgagga gcaccggcgg cgcggggcgg accacgtgtg cgtgcaggtc
      841 attcgcgtcg atgtcgccgc ccccgcgacg ctggagtgga gagcgctcgc gccggtcctc
      901 atcccccggc gctgacgcca ttctcggcgc tcacgccact cccggcggct gatcccatgc
      961 tggcccgcga gagcgcgagg atggcgcggg gcggtccgcg ggatgtcgcc ggtcaggcgg
     1021 cgctgtcgtc tcccggtacc ggcagctcca cgggcgcgaa cgcgggctga tcccagccga
     1081 gctcggcgta tggcggcagg ttcacggctt cgacaccgcg gagctccggg atgtcattcc
     1141 gtaaggatcg ctcgatcccg ctcttcagct gcagcgatgc gacgagccgg ctcggaccgg
     1201 gccgggtcag gccgacgcga agccgggcga tgccgtcctc gacgtcgacg agctccaccc
     1261 gaccgccgct gcgctcgatg tagggcgaca tctcggcgag cttcgccgcg acgcgctgtg
     1321 tgagatcggc catcagccat aggatacgga ggccagatgt gtcatccagc cggctcggtg
     1381 ccgcccgagc tcccgcctga tctccgcccg atctcgggcg gcgcgggcgg cgcggacgtg
     1441 atcctcacga gcgaggacgc cacacggttc cgcgcctacc tcgcgaaggc gaacgccggc
     1501 gacatcggcg tcgtcattgc gccggacgtg cgcgggctgc atcacttcta cgaggagctc
     1561 gcggagcgct tcgccgacgc cggtgtgcac gcgatcgcgt tcgattactt cggccggacc
     1621 gcgggccagg atcgccggcc cgacgatttc ccgttccgcg accacgtgcc gcttacgaag
     1681 cccgaccaga tccaggcgga taccgcgagc gcgatcggcc acctccgcgg cgccacggac
     1741 gcgcgacgca tcttcgtgct cgggttctgc atgggcggac gggtcgcgtt caacgcatcg
     1801 gccgaccagc ccgagatatc aggagcgatc ggattctacg gacgcctctc gacgcgtccg
     1861 ggcgaagagg gcaccgcgcc ggccgaccgc gcgcggcgga tgcgcgcgcc gatcctcggc
     1921 ctcttcggcg ggtccgatcc cggcatcccc gcggatgagg tgcgcgcgtt cgacgcggcg
     1981 ctcaccgcgg agaaggtgcc gcaccacatc gagacctacg ccggggcgcc gcattccttc
     2041 ttcgaccgca cctacgacga ccacaaggac gcatgcgccg acgcctggcg ccgtgccctc
     2101 ggcttcatgc ggaccgggga cccggcggcc cgcgcgtgat cggcgcggtc ctgtacctcg
     2161 cggtcggcac ggtcgtcttc gtcgccatca cgttcctcga gcaggtgccc gtcctcggct
     2221 ggctcggcgg cgtcgtgtcg atcgccgcgt ggatcgcgct ctcgcgcggg ctcgcccgcg
     2281 ggatgggcgc cgatcccctt cggagcggcg cggcgatcat ctggtcgggc gtgctcggcg
     2341 cgttcaccgg attcgtcggg gcgctcacgg cgtggctcgc gcagacggga aacctcttcg
     2401 ggttcacgac gccgccgggt gaccggttcg gcgccgcctt cggcttcgtc ggtgcctcga
     2461 tcgggctcat ctattggccc gtcatcggcg cgctcgtgtg cgtcgcgacg gcgttcatgg
     2521 tcgcgccgcg ccggggcggg gatcggctct agctgacctt ccgccgtttg tggccgacgt
     2581 agtcgacgag cacgtactcg ccgagccgct cgggttccac gtagaaggcg cggccggtgt
     2641 tcagcttcga catctggttc acgaagtcca cgagatagtg gccgcgggcg agcatgaacg
     2701 tgttgatcgt gatctgctcg cgggtgcagc gcacgacctc gcgaagggtc tcctcgaacg
     2761 tgcgcttcgt gggcgggtaa ttgaatcgcg gctccggatc gcccacttcg aagtgcgcgg
     2821 tgggctcgcc gtcggtgatg aggatgatct gcttgttctt gccgcgtgat cgtgacagca
     2881 ggccgcgcgc cgtcgagaac gcgtgctgca tgttcgtgcc gtagacgtac tcgttccacg
     2941 tgagtcccgg cagctccgac ggcttcagct cctgcgcgta cgcggagaac ccgatgatgt
     3001 agaggtcgtc cttggggaac gtggtccgga tcagcgaatc gagcgccagc gtgaccttct
     3061 tcgccgcgag gaagcagccg cggaacagca tcgaccgcga cacgtcgacg aggatcgccg
     3121 tggccgactg cgtcatcagc tccgtgcggt acacgtcgaa gtcctgcggc tgcagcttca
     3181 ccggcacctt cgcgccctca cggaacaccg cgttgcgcac cgtggccgga aggtccagca
     3241 ggaacggatc gccgaactcg tacgtcttga gctcgtcggt cgggtcgccg ccacggccgc
     3301 gcacgcgcga gcggtggctg ccgaacgcgt cgcgcttgag cgtcgcgaag atctcctcga
     3361 gcgagcgctg gccgatcttc cggatgccgc gcggcgtgag ctcgtagcgc tcgccgtctt
     3421 tgcgtatcag cccggcctcc tcgagcagct tgctcatccg ctgaagctgt tcgagcgact
     3481 gccgtgcgcc ggctccggcc agccgctcga cctgatcggg atcgagcggg tcgaggtcgc
     3541 ccgactcgcg agcgccgcgg acgacctcct ccatgtccga cagctgattc atctcgtcca
     3601 tcaacccgag cgcctcggcg agcgtgatcg gctcgtcgcc acgcatcccg tgctcgtagc
     3661 tctcgatcgg catgagctgc gagagggtgt cctggaggtc gttcatcgcg gactgcaggc
     3721 ccggatcgtt gaacgcggcg ccgagcagct cctcgagctg ctgctgcatg tccgtcgaca
     3781 tggactgcca cagcgagcgc atcgcggcca tgccctgcgc gagctgacga aggagatcgt
     3841 cccagtcctt cgcgccgccc gccagctcgg ggtactgacg ctggaacgcc tcgaagtcgg
     3901 gctcaccgcc ttccatgcgc tccttcagcg cgcggttcag gtcgcggatc atctggcgcg
     3961 tgcgctcgag ctgctcgggc gtgaggttct ggagcgagtc gcgcatgttc ttgaagtacg
     4021 agccgagcac ctgctgctgg agctccttca ggagctcctg gtacttctcc gcggccttcg
     4081 gctcgacgaa ctcgtagtcc ttgagcgcgc gcacggcgcg cccgaagtcc tcggggagct
     4141 cgtccagttg cgcgaggcgc tcctgggcga ggcgttccat cgcccgcttc agctgctcgt
     4201 tcggcgcctc ggcggcgcgc cgacgctcga gcccctcacg ctcgaggcgc ttcacgtcct
     4261 cgagcttctc cttcagctgg tcgaaggcgg tggagaggtc gtgccgctgc gcgcgccgct
     4321 gcttcgcgct gcgcagccgc tcgagcagat ccttcatgcc ctccatgcgc ccggggatgc
     4381 cccagcgcga cagccgcgcc atcgcgtcct cgagatcgcc accgctgagc agctcgtccg
     4441 acatcgccgc gagcagctcg tcggcctcga gcgcgtcgag ccgctgcgtc ccgtcccacc
     4501 gcgagtagcg gatgtcgcgc ccgaagtcgt cgagtctggg gatctcgttc acgcgcggaa
     4561 cgtgctccga gactcgcccc gctccttgtt cagcttgcgg ttcagatgca gcccctcgag
     4621 gacgaactcc accgcggcgg cgaccgtcga cggattgccc gcggcaccga gcttcgccac
     4681 ggcgcccttc atgccgggga tctggagggc ctgcttcacg tactcggtcg agggcagcgc
     4741 ggacgagacg tgcatcgtcg cgccgcgcgc gaacgcggcg agcagcgcgt cgaactcgcc
     4801 cagcgagaac gtgcggttga agacgttgag cacggcacgc tgcgcgagac gctcgatgac
     4861 gcgctcctcg gtgacgtcgc ccaccgactc gagctcgatc ttccccgccg tcgaagcgat
     4921 gaccgcgggc agatcgctga tgcgcggcac gaccgtcgtc tcgccgttgc gcagcgcccg
     4981 cttgagcgcg ctcgacacga ggttctcgta gttggccacg gagacgcgga ccgacacgcc
     5041 ggaccgctgc gagatctccg gcgagcgccg cgcgagctgc gatacctccg ccaggatctc
     5101 cttcatatag ccggggacga tcgtcgtgat gccctcgtcg acgaaggtgt tgcgctcctg
     5161 ttccatgatc cccacctcga tgccgatctc cttcgggtag tgggtgcgga tctgcgagcc
     5221 gaagcggtcc ttcagcggcg tgatgatgcg gccgcggttc gtgtagtcct cggggttggc
     5281 cgacgcgacg acgaagaggt cgagcggcag gcggaccttg tagccgcgga tctgcacgtc
     5341 gcgctcctcc atcacgttca gcaggctcac ctgcacgcgc tccgcgagat cggggagctc
     5401 gttcaaggcg aagatgccgc ggtgcgtgcg cggcacgagg ccgtagtgga tcgtgagctc
     5461 gtcggacagg tagcggccct cggcgacctt gatcggatcg acctcaccga tgaggtcggc
     5521 gatggacgtg tccggcgtgg ccagcttctc gccgaagcgc cggtcgcggt ggacccagtc
     5581 gatgggtgtg gcgtcgccga gctcgtcgac caggttgcgg gcgtgggccg acaccgggtt
     5641 cgtggggtcg tcgttgatct cggatcccgc cacgatcggc agccactcgt cgagcaggcc
     5701 cacgagggac cggatcatgc gcgtcttggc ctggccacgc tcgcccagga agatcacgtc
     5761 gtggccg
//
LOCUS       DASTZR010000122         2755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_633909, whole genome shotgun sequence.
ACCESSION   DASTZR010000122 DASTZR010000000
VERSION     DASTZR010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633909"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VFV20_02870"
     CDS             <1..675
                     /locus_tag="VFV20_02870"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEU5287321.1"
                     /translation="GDPRAVPFEVGRLLAPPFVDARRRAIPSRASDRTDAGVMAADTT
                     YLAVVDRDGNAVSMIHSVFKEFGSACVVPGTGIMLNNRLMGFSLDPSSPNALAPRKRP
                     IHTLNTVVALDGTTLRFVFGTPGAHAQVQTNFQLAVGLIDHGFDVQRAIEEPRWYHDA
                     GRALKIEARFPEATRKGLQSKGHEVQLIGDWAEVTGGAQAIAVDANGVFSGGADPRRE
                     GHAAGY"
     gene            710..>2755
                     /locus_tag="VFV20_02875"
     CDS             710..>2755
                     /locus_tag="VFV20_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEU5287322.1"
                     /translation="MTRLWPALLVTVAAVLGACEPAQPQPDAVAKSYAESWQKADYQT
                     MWGLLTEDAKTRVGTEGFVDRLPRIAEEMTARAIEVKVGPSSRPLKNGSPDPNRATIP
                     LDITFHTVRVGDVRRTTTLSLVYVGDKEKGVWKIDWSPEDILPNLTPGRLVRMTRLNT
                     TRGRILARDGTELATFAEGFVVGVVPGQVRSQAGLIASLAPALGVTADFITKQLAQSW
                     VTNDTFVPLKTLSGPAAETAKPKLGVIEGVQLQATRVRAYPTGLASQTLGYLTEASEA
                     EAAKKSARGVQAGDMIGKAGSGLESTLDDVLGGTYGWRLAIIEPNGSPVETLGETAAV
                     PGQDVVLSLDVAVQRAAETALGDRKGAIVAEDPWSGEIRALASRPTFDLNAFISADSA
                     AIAKFSADPNKPLFNRATHGQYPTGSSFKPITAAAALRTGLYKYGDALPCPLRWTGYG
                     PQWVQLNHETVDLGNIDLRTAMARSCNSFFYELGKRLNDRDPNLLPDAAKSFGLGKAT
                     DIDYVLEEEGIVPSPAWKQSYFKNAADQTWNPGDATNLSIGQGFLLATPLQMANYASA
                     LANDGIVWKPRLVTEIRDRSGATVKKLEKTQAGHANATNTELSLIRDTMHAVTSDRDG
                     TVAFVFQNYRVSTAGKSGTAESPAPGKVDAWFVGFASFEQPSLAFAAVLDEFTEVPGR
                     "
BASE COUNT          443 a          975 c          959 g          378 t
ORIGIN      
        1 ggcgatccgc gagccgtccc cttcgaggtg ggccgtctcc tcgcgccgcc gttcgtggac
       61 gcccgtcgcc gcgccatccc gtcgcgcgcg agcgatcgca ccgacgcggg cgtcatggcc
      121 gcggacacga cctacctcgc ggtcgtcgat cgtgacggca acgccgtctc gatgatccac
      181 agcgtgttca aggagttcgg ctcggcgtgc gtcgtccccg ggacgggcat catgctgaac
      241 aaccgcctca tgggcttctc gctcgatccg tcgtcgccga atgcgctcgc gccgcgcaag
      301 cgtccgatcc acacgctcaa caccgtcgtc gcgctcgacg gcacgacgct gcgcttcgtc
      361 ttcgggacgc ccggtgcgca cgcgcaggtg cagacgaact tccagctcgc ggtcggcctc
      421 atcgaccacg gcttcgacgt ccagcgcgcc atcgaggagc cgcgctggta ccacgacgcc
      481 gggcgcgcgc tgaagatcga ggcgcggttc ccggaggcga cgcgcaaggg tctgcaatcc
      541 aaggggcacg aggtgcagct catcggcgat tgggccgagg tgaccggcgg ggcgcaggca
      601 atcgcggtcg acgcgaacgg cgtcttctcc ggcggcgcgg acccgcgccg cgagggccac
      661 gcggccgggt actagcggaa cgccctccgt cgtgcgacgt tctccttcca tgacccgcct
      721 ctggcccgcc ctcctcgtca ccgtcgccgc ggtgctcggc gcatgcgagc cggcgcagcc
      781 gcagcccgac gcggtcgcga agagctacgc ggagtcgtgg cagaaggccg actaccagac
      841 gatgtggggc ctgctcaccg aagacgcgaa gacgcgcgtg ggcacggagg gcttcgtcga
      901 ccggttgccg cgcatcgccg aggagatgac cgcgcgggcg atcgaggtga aggtcgggcc
      961 gtcctcgcgt ccgctgaaga acggctcgcc cgacccgaac cgcgcgacca tcccgctcga
     1021 catcaccttc cacaccgtgc gtgtcggcga cgtccgacgc acgaccacgc tgtcgctcgt
     1081 gtacgtcggc gacaaggaga agggcgtgtg gaagatcgac tggtcgccgg aggacatcct
     1141 tccgaacctg acgcccgggc gcctcgtgcg gatgacgcgg ctcaacacca cgcgagggcg
     1201 catcctcgcg cgcgacggca ccgagctcgc gacgttcgcg gagggtttcg tcgtgggcgt
     1261 ggtgcccggc caggtgcgct cgcaggccgg cctcatcgcg agccttgccc cggcgctcgg
     1321 cgtgacggcg gacttcatca ccaagcagct cgcgcagtcg tgggtcacga acgacacgtt
     1381 cgtcccgctg aagacgctct cgggaccggc cgcggagacg gccaagccga agctcggcgt
     1441 gatcgagggc gtgcagctcc aggccacgcg cgtccgcgcc tacccgacgg gcctcgcgtc
     1501 gcagacgctc gggtacctca ccgaggcgag cgaagccgag gcggcgaaga agtccgcgcg
     1561 cggtgtccag gccggcgaca tgatcgggaa ggccggcagc ggcctcgagt cgacgctcga
     1621 cgacgtgctc ggcgggacgt acggctggcg tctggcgatc atcgagccga acggctcgcc
     1681 ggtggagacg ctcggcgaga ccgcggccgt gccgggccag gacgtcgtgc tctctctcga
     1741 cgtggctgta cagcgggcgg ccgagaccgc gctcggcgac cggaagggcg cgatcgtcgc
     1801 ggaggatccc tggagcggag agatccgcgc gctcgcgagc cggcccacgt tcgatctgaa
     1861 cgcgttcatc agcgccgaca gcgcggcgat cgcgaagttc tcggccgatc ccaacaagcc
     1921 cctgttcaac cgcgccacgc acgggcagta cccgaccgga tcgtccttca agccgatcac
     1981 cgccgcggcc gctctacgca ccggcctgta caagtacggc gacgcgctgc cgtgcccgct
     2041 gcggtggacg gggtacgggc cccagtgggt gcagttgaac cacgagacgg tcgatctggg
     2101 gaacatcgac ctgcgcaccg cgatggcgcg gtcgtgcaac agcttcttct acgagctcgg
     2161 aaagcggctc aacgaccgcg acccgaacct gctgccggac gcggcgaaga gcttcgggct
     2221 gggcaaggcc accgacatcg actacgtgct cgaggaagag ggcatcgtgc cgagtcccgc
     2281 gtggaagcag tcgtacttca agaacgccgc ggaccagacc tggaacccgg gcgatgcgac
     2341 gaacctctcc atcggccagg ggttcctgct cgcgaccccg ctgcagatgg cgaactacgc
     2401 gagcgcgctg gcgaatgacg gcatcgtgtg gaagccgcgc ctcgtgacgg agatccgcga
     2461 ccgctcgggt gcgaccgtca agaagctcga gaagacgcag gccggccacg cgaacgcgac
     2521 gaacaccgag ctctccctca tccgcgacac gatgcacgcc gtcacgtcgg acagggacgg
     2581 cacggtcgcg ttcgtgttcc agaactatcg cgtcagcacg gccggcaaga gcggcaccgc
     2641 cgagtctccg gcgccgggca aggtcgacgc gtggttcgtc ggcttcgcct cgttcgagca
     2701 gccgtcgctc gcgttcgccg ccgtcctgga cgagttcacc gaggtgcctg gacga
//
LOCUS       DASTZR010000123         2245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_738686, whole genome shotgun sequence.
ACCESSION   DASTZR010000123 DASTZR010000000
VERSION     DASTZR010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738686"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1267)
                     /locus_tag="VFV20_02880"
     CDS             complement(<1..1267)
                     /locus_tag="VFV20_02880"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5287323.1"
                     /translation="MAAGTPTRKPPRSSLDLPVTALPGVGAGAEKLLERLEIRTIGDL
                     LWHLPRTYLDYSKFVPLRQLRAGKAQTVEAILGRISQRRTARGLQMTEVELNDPADGM
                     PSGVKATWFGRQFIKERFPEGQLVRLSGDVKWFGRTAQLANPKIEPASAEPVHTGRLV
                     PVYRLTEGLKEGVLRRWLHTAVEGGPKRTPVVREVEERLPADLRMRRELLDIQEALRE
                     IHFPSDWPHNFAARRRLAFDELFTLQLALGQRRARWTKDAKAYPLRVGQADLDAWIAE
                     LPFALTADQRRSVDEIRSDLARTVPMSRLLEGDVGSGKTVVAALAIRIAVASGAQAAL
                     MAPTELLAEQHFRSLGGIFGERGPRIALLTSSITGAARQQTVAALASGGLDAVVGTHA
                     LVEESVTFKKLALAVVDEQHRFGVRQRATF"
     gene            complement(1324..>2245)
                     /locus_tag="VFV20_02885"
     CDS             complement(1324..>2245)
                     /locus_tag="VFV20_02885"
                     /inference="COORDINATES: protein motif:HMM:NF025064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287324.1"
                     /translation="GAYDVQFLVEHPSRPVTAVRKEMEEFGADCVLVVGDETAMKVHV
                     HTLHPDQIIRIGMSAGRLGDVVVEDLDAMTAEHERATGIVVEPPKPTADVGVLAIVPG
                     AGFAAVAASLGATTLRGGATMNPSTEEILTGIREANARHVIVLPNDKNVILAAESAAK
                     LAECAVTVVPTRNVGQGMAALVAFDPAKGPEMAAREMREVAERAHGIEVTRAIRASRI
                     DDQDVREGEAIALIDGRLVAHADDDVVALVEAARRLTDAEVFTLYGGADVDASRVEYA
                     AQRIRASCPRAAVEVVSGGQRHYPFIVTAE"
BASE COUNT          310 a          847 c          770 g          318 t
ORIGIN      
        1 ggaaggtcgc acgctgccgg acgccgaacc ggtgctgctc gtccacgacc gcgagcgcga
       61 gcttcttgaa ggtgaccgac tcctccacca gcgcgtgcgt gccgacgacg gcgtcgagcc
      121 cgccggacgc gagcgccgcg acggtctgct ggcgtgccgc acccgtgatg ctcgacgtga
      181 gcagcgcgat ccgcgggccg cgctcgccga agatcccgcc gagcgagcgg aagtgctgct
      241 ccgcgagaag ttcggtcggg gccatgagcg cggcctgcgc gccggaggcg accgcgatgc
      301 ggatcgcgag cgccgcgacg accgtcttcc ccgaccccac gtcgccctcg aggaggcgcg
      361 acatcggcac ggtgcgcgcg aggtcgctcc ggatctcgtc gaccgaccgc cgctggtccg
      421 ccgtcagcgc gaagggcagc tcggcgatcc aggcgtccag gtccgcctgg ccgacgcgca
      481 gcggataggc cttcgcgtcc ttcgtccagc gcgcgcgccg ctgaccgagc gcgagctgca
      541 gcgtgaagag ctcgtcgaac gcgagccggc gccgcgcggc gaagttgtgc ggccagtccg
      601 acggaaagtg gatctcgcgc agtgcctcct ggatgtcgag cagctcacgc cgcatccgca
      661 ggtcggccgg cagccgctcc tcgacctcgc gcaccaccgg cgtgcgcttc ggcccgccct
      721 ccaccgcggt gtgcagccag cggcgaagca caccctcctt cagaccctcg gtgagccggt
      781 agaccgggac gaggcgtccg gtgtgcaccg gctccgcaga tgcgggctcg atcttcggat
      841 tcgcgagctg cgcggtccgc ccgaaccact tcacgtctcc cgagagacgg acgagctgtc
      901 cctccgggaa gcgctccttg atgaactggc gcccgaacca cgtcgccttc acgccgctcg
      961 gcattccgtc cgccggatcg ttcagctcga cctcggtcat ctggagccca cgcgcggtgc
     1021 ggcgctggct gatgcgcccg aggatcgcct ccaccgtctg cgccttcccg gcgcgcagct
     1081 gccgcagcgg cacgaacttc gagtagtcga gatacgtgcg cggcaggtgc cagagcaggt
     1141 cgccgatcgt gcggatctcg agacgctcga ggagcttctc ggcgcccgcg ccgaccccgg
     1201 gcagcgccgt caccggaagg tcgagcgagg atcgcggcgg cttccgcgtc ggcgttcccg
     1261 cggccacgcg ccaatggtgc cccagcagcg gcccgtcggc acttcgcgcg ggctaacgaa
     1321 tccttactcg gcggtgacga tgaacgggta gtggcgctgc ccgccgctca cgacctcgac
     1381 cgcggcccgc gggcacgaag cgcggatgcg ctgcgcggcg tactcgacac gcgacgcgtc
     1441 cacatcggca ccgccgtaca gcgtgaagac ctcggcatcg gtgagacgcc gcgcggcctc
     1501 aaccagcgcg acgacatcgt cgtccgcgtg cgcgaccagc cgcccgtcga tgagcgcgat
     1561 ggcctcgccc tcgcggacgt cctgatcgtc gatccggctc gcccggatcg cgcgggtgac
     1621 ctcgatcccg tgcgcgcgct cggccacctc gcgcatctcg cgcgcggcca tctccggccc
     1681 cttcgccgga tcgaacgcca cgagcgcggc catcccctgc ccgacgttcc gcgttggcac
     1741 cacggtcacc gcgcattccg cgagcttcgc cgccgactcc gcggcgagga tgacgttctt
     1801 gtcgttcggc aggacgatga cgtgccgcgc gttcgcctca cggatgccgg tgaggatctc
     1861 ctcggtggac gggttcatcg tcgcgccgcc gcgcaacgtc gtcgcgccga gcgaggcggc
     1921 cacggccgcg aagccggcgc cgggcacgat cgcgagcacg ccgacgtcgg ccgtcggctt
     1981 cggtggctcg acgacgatcc cggtcgcgcg ctcgtgctcg gcggtcatcg cgtcgaggtc
     2041 ctcgacgacg acgtcgccga gacgacccgc gctcatgccg atgcggatga tctgatcggg
     2101 atgcagcgtg tggacgtgca ccttcatcgc cgtctcgtcg ccgacgacga ggacgcagtc
     2161 cgcgccgaac tcctccatct ccttgcggac cgcggtcacg ggccggctcg gatgctccac
     2221 caggaactgg acgtcgtacg cgccc
//
LOCUS       DASTZR010000124         2546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_242674, whole genome shotgun sequence.
ACCESSION   DASTZR010000124 DASTZR010000000
VERSION     DASTZR010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242674"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1838)
                     /locus_tag="VFV20_02890"
     CDS             complement(<1..1838)
                     /locus_tag="VFV20_02890"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU5287325.1"
                     /translation="MPAWTPSDAYLNRSRLRAFAKHHGYDEYASFLRWSQTDLDAFWR
                     GVDRDLGLVWRKRYDRVLDDSRGIPWATWWTGGRMNYVNSALHKRIGPDRVAVIAEGE
                     EGTVRRISYGQLRADVVAFARGLRGLGLGRGDRIGIFMPMTYECVVAVLAVGYLGAVY
                     IPIFSGYGAEAIAGRLRDCDASALVCADGFYRRGQVVPMKETADAALASVPSVRHLVV
                     AERVGRAYAKLSRDVAWRDVMHAASGSAAWEPEDTSAEDPFMVIYTSGTTGKPKGAVH
                     VHGGFPVKAAQDLAHCFDLQEGDVILWSTDIGWMMGPWLIAGGLMLGATIVVYDGTPD
                     FPDASRMWSIVERHRVTHLGISPTAIRGLMRAGEDPVRSHDRSSLFVLGSTGEPWNPD
                     PWWWYFNNVGETRCPIINYSGGTEVSGGIVGCTTWTPLQPGSFVGPCPGIDADVVDEQ
                     GRSVRGAVGELVIRKPWPGMTRGFWKDGHDETSRYIQTYWSRLPNVWVHGDWARIDAE
                     GFWYIEGRSDDTLKIAGKRVGPAEVESAAVAHPAVSEAAAVGVPHEIKGEAIVVFCVL
                     RPDHAPSDALAREVQDTVAGQLGKPLRPEAVRFVAQLPKTRNAKILR"
     gene            complement(1849..2181)
                     /locus_tag="VFV20_02895"
     CDS             complement(1849..2181)
                     /locus_tag="VFV20_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019989570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEU5287326.1"
                     /translation="MRRVAAVLALRCPRCLEGRVWRGFLDMHERCAVCGLVYEREPGY
                     FTGAMVVSYVIAVPTFGLIVIGLLAAGVSTAPALLVGGALYLMLAPFILRYSRVIWLH
                     FDWLVDPI"
     gene            complement(2181..>2546)
                     /locus_tag="VFV20_02900"
     CDS             complement(2181..>2546)
                     /locus_tag="VFV20_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287327.1"
                     /translation="LGATAVHAAARVAGARQPFAPLLLAFAYGTALTLVPANLATLLL
                     GVGQGVGPQTGQLVSVACLIWLLVIVHRTIQAHYEVTGERALRILIVAVVVFYLVPFA
                     LIVASAVAIIIAAVVLEYF"
BASE COUNT          423 a          908 c          874 g          341 t
ORIGIN      
        1 cgcaggatct tcgcgttgcg cgtcttcggg agctgcgcga cgaagcgcac ggcctcgggc
       61 cggagcggct tgccgagctg acccgcgacc gtgtcctgca cctccctcgc gagcgcgtcg
      121 ctcggggcgt ggtccggccg cagcacgcag aacacgacga tcgcttcacc cttgatctcg
      181 tgcggcacgc cgaccgcggc ggcctcgctc accgccgggt gtgccaccgc tgcggactcg
      241 acctccgccg gaccgacgcg cttgcccgcg atcttcagcg tgtcgtcgct gcgcccctcg
      301 atgtaccaga acccctccgc gtcgatgcgc gcccagtcgc cgtggaccca gacgttcggc
      361 aggcgtgacc aataggtctg gatgtagcgg ctcgtctcat cgtgcccgtc cttccagaac
      421 ccgcgcgtca tccccggcca cggcttccgg atcacgagct cgccgaccgc gccgcgcacc
      481 gaacggccct gctcgtcgac gacgtcggcg tcgatgcccg ggcagggccc gacgaacgac
      541 ccgggctgga gcggtgtcca ggtggtgcag ccgacgatgc ccccggacac ctcggtgccg
      601 ccggagtagt tgatgatcgg acagcgcgtc tcgccgacgt tgttgaagta ccaccaccac
      661 gggtccgggt tccacggctc gccggtggac ccgaggacga agagcgacga gcgatcgtgg
      721 ctccgcaccg ggtcctcgcc cgcgcgcatc agcccgcgga tggccgtcgg cgagatgccc
      781 agatgcgtga cgcggtgccg ctcgacgatg ctccacatgc gcgacgcgtc ggggaagtcg
      841 ggcgtcccgt cgtagacgac gatcgtcgcg ccgagcatga gcccgccggc gatgagccag
      901 gggcccatca tccagccgat gtccgtggac cacaggatga cgtcgccctc ctgcaggtcg
      961 aagcagtgcg cgaggtcctg cgcggccttc accgggaatc caccgtgcac gtggaccgcg
     1021 cccttcggct tgccggtcgt accggaggtg tagatgacca tgaagggatc ctcggcggag
     1081 gtgtcctccg gctcccacgc ggcggatccg ctcgccgcgt gcatgacgtc gcgccacgcg
     1141 acgtcgcgtg acagcttcgc gtacgcccga ccgacgcgct ccgcgacgac cagatgccgg
     1201 acgctcggca cgctcgcgag cgccgcgtcg gcggtctcct tcatcggcac gacctgaccg
     1261 cggcggtaga acccgtcggc acagacgagc gcggacgcgt cgcaatcacg caggcggccg
     1321 gcgatggcct cggcgccgta tccggagaag atcgggatgt acacggcgcc gaggtagccg
     1381 acggcgagca ccgcgacgac gcactcgtac gtcatcggca tgaagatgcc gatccggtcg
     1441 cctcggccca gaccgagccc gcgcaggccc cgtgcgaacg cgacgacgtc ggcgcgcagt
     1501 tgtccgtacg agatacggcg gacggtgccc tcctcgccct ccgcgatgac cgcgacgcgg
     1561 tcgggtccga tccgcttgtg cagcgcgctg ttcacgtagt tcatgcgccc gccggtccac
     1621 caggtcgccc acgggatgcc ccgtgagtcg tcgagcacgc ggtcgtagcg ctttctccag
     1681 acaagaccca ggtcgcggtc gaccccacgc cagaacgcgt cgagatcggt ctgcgaccag
     1741 cgcaggaacg acgcgtactc gtcatatcca tggtgcttcg cgaacgcgcg cagtctgctg
     1801 cgattcagat aggcgtcact cggggtccac gcgggcatcg cgacaacgtt agatcggatc
     1861 gacgagccag tcgaagtgca gccagatcac gcgcgagtag cggaggatga acggcgcgag
     1921 catcaggtac agggcacccc cgacgaggag cgccggcgct gtggacacgc cggccgcgag
     1981 caacccgatg acgatgaggc cgaaggtggg caccgcgatg acgtacgaga cgaccatcgc
     2041 acccgtgaag tagcccggtt cccgctcgta gacgagaccg cacaccgcac agcgctcgtg
     2101 catgtccagg aaaccgcgcc agacccggcc ttccagacac cgcgggcagc gcagcgccag
     2161 cacggccgcg acgcgccgca ctagaagtac tcgaggacga cggcggcgat gatgatcgcg
     2221 actgccgagg cgacgatcag cgcgaacggc acgaggtaga acacgaccac ggcgacgatg
     2281 aggatccgca gggcgcgctc gccggtgacc tcgtagtgcg cctggatcgt gcgatgcacg
     2341 atgacgagca gccagatgag acaggcgacg ctgacgagct ggcctgtttg cggtccgacg
     2401 ccctgcccca cgccgaggag gagcgtcgcg aggttcgccg gcacgagcgt gagcgcggtc
     2461 ccgtacgcga aggcgagcag cagcggcgcg aacggctgtc gcgcgcccgc gacgcgcgca
     2521 gcggcgtgca cggccgtcgc gccgag
//
LOCUS       DASTZR010000125         2968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_659408, whole genome shotgun sequence.
ACCESSION   DASTZR010000125 DASTZR010000000
VERSION     DASTZR010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659408"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VFV20_02905"
     CDS             <1..808
                     /locus_tag="VFV20_02905"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU5287328.1"
                     /translation="GAQLERGDLADEEALRRGMRGADAVVHGAAIYAVGISAAQRAEM
                     FDANVRGAERVLSTAAALGVRRILHVSTIAVFGNTRGAVVDERYQRSGPYTSYYEETK
                     FRAHEIARRLAERGAPILIAQPGQVYGPGDHSGFGRFLRAFARGRLPLVPFPDLGMNL
                     VHVDDVSDGIVRVLDRGAAGASYPLGGEIVRVKQAFETLARVLGRRPPRFDAPYLPLR
                     LAALFRADVREILTSSRGVTFWATDARARAELGYAPRDLETGLRETFATG"
     gene            complement(1054..2166)
                     /locus_tag="VFV20_02910"
     CDS             complement(1054..2166)
                     /locus_tag="VFV20_02910"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU5287329.1"
                     /translation="MAVVGAKRRRARRVEIRVPFTSGTDLAFGVSLRVPRRRRRTRGV
                     GVVRHWGAAVGRSALLVLNLVMPPAIVASTTQLADIADAAFPPPAPAALASASSMPRP
                     VLQARRTLTTVTTPDPPQVRAVVVAPGETLLSIAARFGVAPQTIAYDNGIRDSAELRA
                     GQSLLVPPFDAAIHVLGPKETISEVASRFGVAAETVRTLNRIASDDGDATAGRLLLLP
                     VPDARYPGFRLRLSDPPRVLAPRVRWPTDGVITQLYSPGHQGVDIAAPYGAPIVASDS
                     GTVSFVGDRGPGGLAVCVYQDWGLETCSYHASATYVEVGERVIAGQRIAAIGTSGVTT
                     GPHVHWEARTNGALVDPRTYASYAATPAVRGATGSP"
     gene            complement(2233..>2968)
                     /locus_tag="VFV20_02915"
     CDS             complement(2233..>2968)
                     /locus_tag="VFV20_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5287330.1"
                     /translation="IYPRLATRYRTVAYDQRGHGKSGRATAYSLVDFGADAVRVVRQV
                     VKGPTVVIGHSLGSLCAIAAAAEAPDLVVGLVLEDPPIAYSKNWDRDALSRMRDALAG
                     ATDDKVLLRFVQGLPLASPGPRGERTFGESRGFYAAERVLTYYRDLDPAIPGSRAGQP
                     DQPGIDALAEWLPKVKCPVLVIAGEPRLGSVLDDASEWKLKRAVKDLTVKRFPGTGHL
                     IHGYRPEQFIENVEPFLRRLRTPASV"
BASE COUNT          410 a         1056 c         1100 g          402 t
ORIGIN      
        1 cggtgcccag ctcgagcgcg gcgatctcgc cgacgaggag gccctgcgcc gcgggatgcg
       61 tggcgcggac gccgtcgtgc atggcgcggc catctacgcc gtcggcatca gtgccgcgca
      121 gcgggccgag atgttcgacg cgaacgtccg cggcgcagaa cgcgtgctct cgaccgccgc
      181 ggcgctcggc gtgcgccgga tccttcatgt ctcgacgatc gccgtgttcg ggaacacgcg
      241 cggcgcggtc gtcgacgagc gataccagcg gagcggaccg tacacctcgt actacgagga
      301 gacgaagttc cgcgcccacg agatcgcgcg ccggctcgcg gagcgcggtg cgccgatcct
      361 catcgcgcag cctggccagg tgtacgggcc gggagaccat tccgggttcg gacggttcct
      421 gcgcgcgttc gcgcgcggtc gcctgcccct cgtgccgttc ccggatctcg gtatgaacct
      481 cgtgcacgtg gacgacgtat cggacggcat cgtgcgcgtc ctcgaccgcg gcgccgccgg
      541 cgcgagctat ccgctcggcg gcgagatcgt gcgcgtgaag caggcgttcg agacgctggc
      601 gcgcgtgctg ggacggcgtc cgccgcgctt cgacgcgccg tatctgccgc tgcgcctcgc
      661 cgcgctgttc cgcgccgacg tccgcgagat cctcacctcg tcacgcggcg tcacgttctg
      721 ggcgaccgac gcgcgtgctc gcgccgagct gggctacgcg ccacgcgatc tggagaccgg
      781 gctgcgggag acgttcgcga cgggctagct gaggtctccc agaccccagt cgcgtccgcg
      841 tggcggcggg tcctgtcccg ctccgggcgt cgttctcccc tcgcgaagtc cggctccccg
      901 gcccgcccgt cggccggtgc gcgtgatgga ccgcaccgta cgggcgtccg gggccccgcg
      961 gacttcgctt cggtgagacc aacgccctgc gcgtgccacc cgccgccacg cggatgtgac
     1021 cgacgcgcgt caggagaccc caacgagccg acgctagggc gagccggtcg cgcctcgcac
     1081 agcgggcgtc gcggcgtagc tcgcgtaggt ccgcgggtcc acgagcgcac cgttcgtgcg
     1141 cgcctcccag tggacgtgcg gcccggtcgt gacgccgctc gtcccgatgg cggcgatgcg
     1201 ctgccctgcg atcacgcgct cgccgacctc gacatacgtc gccgacgcgt gatacgagca
     1261 ggtctcgaga ccccagtcct ggtacacgca caccgcgagg ccgcccggtc cgcggtctcc
     1321 gacgaagctc accgtgcccg agtcgctcgc gacgatcggc gcgccgtacg gcgcggcgat
     1381 gtcgacgccc tggtgccccg gcgaatacag ctgcgtgatg acgccgtccg tgggccagcg
     1441 cacgcggggc gccaggacgc gcggcgggtc cgacaggcgc aggcggaatc cgggatagcg
     1501 cgcatccggg accggcagga gcagcagccg gcccgcggtc gcgtcgccgt catccgacgc
     1561 gatgcggttc agcgtccgca cggtctcggc ggcgaccccg aaacgcgacg cgacctccga
     1621 gatggtctcc ttcggtccga gcacgtggat cgccgcgtcg aatggtggca ccaacagtga
     1681 ctgaccggcg cgcagctcgg cgctgtcgcg gatgccgttg tcgtacgcga tcgtctgcgg
     1741 cgcgacgccg aagcgggcgg cgatggagag cagcgtctca cccggtgcga cgacgacggc
     1801 ccgcacctgt ggcgggtcgg gcgtcgtcac ggtggtcagc gtccgccgcg cctgcagcac
     1861 gggacgcggc atgctggagg cgctcgcgag cgcggcggga gcgggcggtg ggaacgccgc
     1921 gtcggcgatg tcggcgagct gcgtcgtgct cgccacgatg gcggggggca tcacgaggtt
     1981 gaggaccagc agcgcggagc ggcccacggc ggcaccccag tggcgcacca cgccgacgcc
     2041 ccgtgtgcgg cgacggcgac gcgggacacg cagcgagacc ccgaacgcga gatcggtccc
     2101 gctcgtgaag gggacccgga tctcgacacg acgggcgcgc cgccgcttcg ctccgaccac
     2161 ggccacgcct gcgcgacggg caagcagcgt gccgtacgtg cgtatgacgg gcgaacgagc
     2221 gaggacggat gttcagacgg acgcgggggt gcgcaggcgg cgaaggaagg gctcgacgtt
     2281 ctcgatgaac tgttccggcc ggtagccgtg gatgaggtgg ccggtccccg ggaaccgctt
     2341 gaccgtcaga tccttcaccg cacgcttcag cttccattcg gaggcgtcgt cgaggacgga
     2401 accgaggcgc ggctcccccg cgatgacgag caccgggcac ttcaccttcg gcagccactc
     2461 tgcgagcgcg tcgatgcccg gctggtcggg ttgacccgcg cgcgatccgg ggatcgcggg
     2521 atcgaggtca cggtagtagg tgaggacgcg ctcggcggcg tagaagccgc ggctctcacc
     2581 gaacgtgcgc tctccgcggg gcccggggga tgcgagcggc aggccctgca cgaagcgcag
     2641 gagcaccttg tcgtcggtcg cgccggcgag cgcgtcgcgc atacgcgaca acgcgtcacg
     2701 gtcccagttc ttcgagtagg cgatcggtgg atcctcgagg acgagcccga cgacgaggtc
     2761 gggcgcctcc gcggcggcgg cgatcgcgca gagcgatccg agcgagtgtc cgatcacgac
     2821 ggtcgggccc ttcacgacct gtcgcaccac gcgcaccgcg tcggcaccga agtcgaccag
     2881 cgaatacgcg gtcgcgcggc cgctcttccc atggcctcgc tggtcgtacg cgacggtccg
     2941 atagcgggtc gcgagcctcg gatagatg
//
LOCUS       DASTZR010000126         2342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1026185, whole genome shotgun sequence.
ACCESSION   DASTZR010000126 DASTZR010000000
VERSION     DASTZR010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026185"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VFV20_02920"
     CDS             complement(<1..264)
                     /locus_tag="VFV20_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020807085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU5287331.1"
                     /translation="MISLLAARTPTAVREIPLEKVKPNPGQPRMTFHQDTLNELASSV
                     KEHGVLQPILVRPSGEGYEIIAGERRWRASKLAGKETIPAIVER"
     gene            complement(344..1111)
                     /locus_tag="VFV20_02925"
     CDS             complement(344..1111)
                     /locus_tag="VFV20_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006917554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5287332.1"
                     /translation="MGKIIAIANQKGGVGKTTTAINLGGALAQIGKRVLCLDLDPQAN
                     LTVGLGIDINSVERNIANVLVDPDVSLESIIRPSKTRNVDVVPATLELSSAEVELFNA
                     IGREMALRDKVTPAIAQRYDYILIDCPPTLGLLTLNALVASDGVIIPVQTQYYALKGV
                     AALLRIIKTVQARLNPNLKVYGLLPTFYDSRTILARDMLDSLKDLGDHQVFATVIRQS
                     VKIGEAPTAGVPITEYDKKSDAAKAFVDLAKEVVRAH"
     gene            1375..1446
                     /locus_tag="VFV20_02930"
     tRNA            1375..1446
                     /locus_tag="VFV20_02930"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1407..1409,aa:Ile,seq:cat)
     gene            1460..>2342
                     /locus_tag="VFV20_02935"
     CDS             1460..>2342
                     /locus_tag="VFV20_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEU5287333.1"
                     /translation="MTISISDIERAREMTAPHLHRTPMLTSRTLSERIGAAAFLKAES
                     FQRTGSFKPRGALFAVASLTAEQRAKGIVTMSAGNAAQAIAYAGTAAGVKVTVAMPES
                     APRLKVDATRGYGAEVRFAADMTGLRALVQALQDQTGAYFLHPYDDDGMITGHGSLGL
                     EILEDVPEADVVVVGVGGGGLISGVASAVAAKRPACRIVGVEPEGAAAVRLALDAGRP
                     VPVSPKTIADGLAAPVAGTRNLEIVRRLVADVVVISDEQITEGLRFLAERAKLVAEPA
                     GAAAVGALLAGRVPVKRV"
BASE COUNT          343 a          795 c          817 g          387 t
ORIGIN      
        1 ccgctcgacg atcgccggga tggtctcctt gcccgcgagc ttcgacgccc gccagcgacg
       61 ttcgccggcg atgatctcgt agccctcgcc ggacggccgc accaggatcg gctgcagcac
      121 gccgtgctcc ttcaccgagg acgcgagctc gttgagagtg tcctggtgga acgtcatgcg
      181 cggctgaccc gggttcggct tcaccttctc gagcgggatc tcgcgcacgg ccgtcggcgt
      241 gcgggccgcg agcaggctga tgatcccggg ctgcgcgtcg gtctcctgcg agaagatccg
      301 gctccccgcg tcgcggaacg acttgccctt cgatgtcgtg gggctagtga gcacggacga
      361 cctccttggc cagatcgacg aacgccttcg cagcgtccga cttcttgtcg tattcggtga
      421 tcggcacgcc ggcggtcggc gcctcgccga tcttcacgga ctggcggatg acggtggcga
      481 agacctggtg atcacccaga tccttgagcg agtcgagcat gtcgcgcgcg aggatcgtgc
      541 ggctgtcata gaaggtgggg agcaggccgt agaccttgag gttgggattg agccgcgcct
      601 ggacggtctt gatgatccgc agcagcgcgg cgacgccctt cagcgcgtag tactgcgtct
      661 gcacggggat gatcacgccg tccgacgcga cgagcgcgtt gagcgtgagc agccccagcg
      721 tcggcgggca gtcgatcagg atgtagtcgt agcgctgcgc gatcgccggc gtgaccttgt
      781 cgcggagcgc catctcgcgt ccgatcgcgt tgaagagctc gacctcggcc gacgacagct
      841 cgagcgtcgc gggcacaaca tcgacgttcc gtgtcttcga gggacggatg atcgactcga
      901 gcgacacgtc cgggtcgacg aggacgttcg cgatgttgcg ctcgaccgag ttgatgtcga
      961 tcccgagccc gacggtcagg ttcgcctgcg gatcgaggtc gaggcaaagg acgcgctttc
     1021 cgatctgcgc gagcgcgccg ccgaggttga tggctgtcgt ggtcttcccg acgccaccct
     1081 tttgattcgc gatcgcgatg atcttgccca cggcttccct ccgcgcgacg tctccccaga
     1141 acgtgcacgc gcgtgcggaa gctgcacgcg actcccctcg cgaacgctcg ctgcgtagca
     1201 tagtggccca gagcgcgaat ggagcgcgtt ttccggcgat agggccgtac tcgctcgctc
     1261 accggcgcgt cgtcgccgct agcatggccg ccgcgacggg ggttccgcat cgcttcgctc
     1321 acggccccgt cgtcggccgg atccgtgagc aagctcgcgc ccacggcctt gctagggccc
     1381 gtagctcagt ggtagagcgg cggactcata atctgctggt cgcaggtccg atccctgccg
     1441 ggcccacaat cccctccaga tgaccatttc catatccgac atcgagcgcg cccgcgagat
     1501 gacggcgccg cacctacacc ggacacccat gctcacgagc cgcaccctga gcgagcgcat
     1561 cggtgcggcg gcgttcctca aggccgagag cttccagcgc accggttcct tcaagccccg
     1621 cggggcgctg ttcgcggtcg ccagcttgac cgccgagcag agggcaaagg gcatcgtcac
     1681 gatgagcgcc ggcaacgccg cgcaggcgat cgcctatgcc ggcactgccg cgggcgtgaa
     1741 ggtcacggtc gcgatgccgg agagcgcgcc gcgcctcaag gtggacgcga cgcgcggcta
     1801 cggggccgag gtccgcttcg cagcggacat gactggcctg cgcgcgctgg tccaggcgct
     1861 ccaggaccag accggcgcgt acttccttca tccatatgac gacgacggca tgatcaccgg
     1921 gcacggctct ctcggcctcg agatcctcga ggacgtgccg gaagccgacg tggtggtggt
     1981 cggcgtgggc gggggcggtc tcatctccgg tgttgcgagc gccgtcgcgg cgaagcggcc
     2041 ggcatgccgc atcgtcggtg tcgaaccgga gggcgcggcg gccgtgcgcc tcgcgctgga
     2101 cgcggggaga cccgtgccgg tgtcgccgaa gacgatcgcc gacgggctcg ccgcaccggt
     2161 cgcgggtacg cgcaacctcg agatcgtgcg ccgtcttgtg gccgacgtcg tcgtgatctc
     2221 cgacgagcag atcaccgagg ggctgcgctt cctcgccgag cgcgcgaagc tcgtggccga
     2281 accagcgggc gccgccgccg ttggcgcgct cctcgcggga cgcgtccccg tgaagcgcgt
     2341 gg
//
LOCUS       DASTZR010000127         2446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1130428, whole genome shotgun sequence.
ACCESSION   DASTZR010000127 DASTZR010000000
VERSION     DASTZR010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130428"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VFV20_02940"
     CDS             complement(<1..468)
                     /locus_tag="VFV20_02940"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU5287334.1"
                     /translation="MRRISAISLAIMFVAAACGGGGGLLGGGDSSNQAPGKDVKPAAK
                     ITWWHAMSGVNGDALNKIVDGFNKSGKGITVEAVFQGNYDDLLAKLNTAIASNAAPAL
                     VQVYDIGQVYMRDSGQIVPMQAFIDRDKFETKDFEPAVINYYKYQDKLQSMPFN"
     gene            complement(488..1345)
                     /locus_tag="VFV20_02945"
     CDS             complement(488..1345)
                     /locus_tag="VFV20_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU5287335.1"
                     /translation="MATGRGGHHRRRRRIAPRVIATYVLLVLASLLVAFPLLLALSYS
                     LMSESAIATFPPPVIPTEPTLANYGTVFATIPIGRYLLNSLVVSSAVVIGQLITASLA
                     AYAFSFLTYPGRQLLFFLFLSTLMIPWEATIIPNYITIRSIGWLDTYQGLAMPFMATA
                     FGTFLLRQAFLQIPRELWDSARIDGASSFHFFRTMVVPLSRPALGTVAIYAFLSTYNQ
                     YFWPLLITNQPLMRTTQVGIAQLRFEETLRWGLVMAGVIMVAAPTLALLVIGQRQLIR
                     GLTAGAVKG"
     gene            complement(1345..2232)
                     /locus_tag="VFV20_02950"
     CDS             complement(1345..2232)
                     /locus_tag="VFV20_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU5287336.1"
                     /translation="MRLSSRNIRSLLVALMYLAPSLVIFIAFVFVPLGRTIELSLFNT
                     RVTGVPTTFAGLDHYSELLSSDAFRTGMFATLIFALYTVPIGIALGLVLAVLLNQRLR
                     GISVFRTLTSSTIAVSAAVGSLIWLLLFNPSLGLLNYVLSLAGIRGPEWLIQPTTAII
                     AVSLTTVWLMLGTNVIVLLAGLQGVPEELYEAARIDGARGLRLFGSITVPMVSPSLFF
                     LLVVDTIAVLQAFTQIHVMTRGGPVDTTRVLVYSIYLDAFQNFQFGFASAQAVILFGL
                     VLALTIVQFRFIERRVHYQ"
BASE COUNT          474 a          730 c          858 g          384 t
ORIGIN      
        1 gttgaacggc atgctctgca gcttgtcctg gtacttgtag tagttgatga ccgcgggctc
       61 gaagtccttc gtctcgaact tgtcgcggtc gatgaacgcc tgcatcggca cgatctggcc
      121 gctgtcgcgc atgtagacct ggccgatgtc gtacacctgc acgagcgcgg gtgccgcgtt
      181 cgacgcgatg gccgtgttca gcttcgcgag aagatcgtcg tagttcccct ggaacaccgc
      241 ctcgacggtg atgcccttcc cgctcttgtt gaagccgtcg acgatcttgt tgagcgcgtc
      301 accattgacg cccgacatcg cgtgccacca ggtgatcttc gccgccggct tgacgtcctt
      361 gccgggagcc tggttgctcg aatcgccgcc cccaaggaga ccgcctccgc cgccgcacgc
      421 ggcggcgacg aacatgatcg cgagcgagat cgctgaaatc ctccgcacgg atcgtcctcc
      481 ttcttgctta gcccttgacc gcacccgcgg tcagcccgcg tatgagctgt cgttgtccga
      541 tgaccagaag cgcgagcgtc ggtgccgcga ccatgatgac accggccatc acgaggcccc
      601 accgcagggt ctcctcgaag cgtagctgcg cgatgcccac ctgagtcgtc cgcatgagtg
      661 gctggttcgt gatgagcagc ggccagaagt actggttgta ggtggagagg aacgcgtaga
      721 tcgcgacggt cccgagagcg ggccgcgaga gcgggacgac catggtgcgg aagaagtgga
      781 acgacgatgc gccgtcgatg cgcgcggagt cccacagctc tcgcgggatc tgcaggaacg
      841 cctgccgcag caggaaggta ccgaaggccg tcgccatgaa cggcatcgcc aggccctggt
      901 acgtgtcgag ccacccgatc gaacggatgg tgatgtagtt cgggatgatc gtcgcttccc
      961 acgggatcat gagcgtcgaa aggaagagga agaagagcag ctgtcggccg gggtaggtga
     1021 ggaacgagaa cgcgtatgcg gcgaggctcg cggtgatgag ctgcccgatc acgacagcgc
     1081 ttgacacgac caggctgttc aagagatagc ggccgatcgg gatcgtcgcg aagaccgtcc
     1141 cgtagttggc gagcgtcggc tccgtgggga tcaccggcgg cgggaacgtc gcgatcgccg
     1201 actcgctcat gagcgagtag gaaagggcaa gcagcagtgg gaacgcgacg aggagtgagg
     1261 ccaggacgag gagtacgtag gtcgcgatca cgcgcggcgc gatgcgccgc cggcgtcgat
     1321 ggtggccgcc gcgtcccgtc gccatcattg atagtgcact cgtcgctcga tgaagcggaa
     1381 ctggacgatg gtgagcgcga gcacgagccc gaacaggatc accgcctgcg cgctcgcgaa
     1441 gccgaactgg aaattctgga acgcgtcgag atagatgctg tagacgagga cgcgagtggt
     1501 gtcgaccggc ccgccgcgcg tcatgacgtg gatctgcgtg aacgcctgca gcaccgcgat
     1561 cgtatcgacg acgagcagga agaagagcga tggcgagacc atcggcacgg tgatgctgcc
     1621 gaagaggcgc agaccgcgtg cgccgtcgat gcgcgccgcc tcgtagagct cctcgggcac
     1681 gccctggaga ccggcgagca gcacgatgac gttcgtgccg agcatgagcc acaccgtggt
     1741 gagtgagacg gcgatgatgg cggtcgtcgg ctggatgagc cattcgggtc cgcggatgcc
     1801 ggcgagtgac agcacgtagt tcaggagccc gaggctcggg ttgaacagca agagccagat
     1861 gagcgacccg accgccgccg agaccgcgat ggtgctcgag gtcagggtgc ggaacacgct
     1921 gatcccgcgc aggcgctggt tgagcagcac cgccaggacg agacccaggg cgatgccgat
     1981 cggcacggtg tacagcgcga agatcagcgt cgcgaacatc ccagtgcgga acgcgtcgga
     2041 cgagagcagc tcgctgtagt ggtcgagacc ggcgaaggtc gtgggaacac cggtcactcg
     2101 tgtgttgaag aggctgagct cgatcgtcct gccgagcggc acgaacacga acgcgatgaa
     2161 gatgacgagc gacggcgcca gatacatgag cgcgacgagc agggatcgta tgttgcgaga
     2221 cgagagccgc atcaggtgcg ggcgatggta ccgctcgcca gagcctcgtt cgctctcacg
     2281 aggacgctcc cgcgatctcc agcagatcgg tgaggatcgc gtaggccacc tgctccacgt
     2341 gcgggtcgtg gaggtgcacg gcgaccttgc cggcgagatc ggtctccagc acgacgccca
     2401 tcgagaagcc atccaccgcg tagagcgggt cgagcggctc gagccg
//
LOCUS       DASTZR010000128         4570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1052493, whole genome shotgun sequence.
ACCESSION   DASTZR010000128 DASTZR010000000
VERSION     DASTZR010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052493"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VFV20_02955"
     CDS             complement(<1..203)
                     /locus_tag="VFV20_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014174603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 family protein"
                     /protein_id="HEU5287337.1"
                     /translation="MRREYPTHLSYWITGPGPLTPPRELHPAFYGSFDWHSCVEMHWL
                     LVRLLRAVPDLAPAREARAALDE"
     gene            complement(278..3580)
                     /locus_tag="VFV20_02960"
     CDS             complement(278..3580)
                     /locus_tag="VFV20_02960"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU5287338.1"
                     /translation="MQQQGQERRLVTVLFADFAGFTQLADQMDPEELQVLVSGIFEDL
                     AEEAVRNDGTIEKFIGDAIFVIFGAPVAHEDDPQRALRTALAMQKVFADHAARVKKDR
                     GIEFGLRIGVHTGMVVAGSVRSVAEFGVMGDTVNTASRIQAAAGPTEIYVSQTTFRLT
                     NREFSFREVGPVDLKGKDKPVLVYALTGERTDARPMIDVAAPLVGRWMELSRLDLAYQ
                     SSRMGHTEVVLITGEPGIGKTRLLSEFIGLATASEDGASAPEAPRVLRWTFSRVNQRS
                     YAGFIEPLLVELKVDTAMPNGQEVVAERLAALGFANPLAVAPVLAQFLHLAGAPEPAS
                     DTEEWRRSMFIVVYDVIAALARERPLLYVLEDLHFADAASLDLLWFLASRASRVPLLF
                     LLAQRMGPGAPEPRPSRTNFTQLVLEPLPDEEAARIVEATFDWIPDELRDRIVARAGG
                     NPFFIEESLRSLVESGAVQKDETGEWRLRDRPSMLEVPTTLHAVVAARIDRLPPNARE
                     CIQLASVIGQRFGDRVLREAGGNRLADAVDQLIAADLVLEAAPGERREGRYRFKHAVV
                     QEVAYNTLLVRRRSEMHGRVAAAYEKVLSETELREFYPALAHHFLLADVPEKAVAYSW
                     KAAQRATAIHAYVEALRFAEQALEIYEKLGKTDQAVEALYLIARIRRYRGENDGALAA
                     YERALSLLEAQDPNGPAVATMLAHMAELCTRWDAKHPDLQGLIDRGLAIVDGERNREK
                     VLLLAAKAFMARKGPKPTDADWEASLATAKEALAIAEDLGSLREVSLCLDAVGYAYGE
                     LGNFRESYAQNQRRLPIAKSLQDSDELIDAHTMVAVAALVLGNFPEVLENAVAASEIA
                     AETEKPRLAAQALQVEAYARLLSGDFPGTIEAAARRDRVMPQVKGHNALAIAAAAAAA
                     MALPDEKTLRERLVEIEAGPVDIAACDFLTAVYGLREAESAYRAIRSAGYPKGGVDLV
                     VLGPLAVLAAARWRIEDPAFAERVERAVERSGHARGRALLVQAEGVAAQKAGEHTKAS
                     KLLFDAVQSFATLKLDYERAVALADLARSLRETGRRDQAQTFCDEAKAIAERLSAVAL
                     RAAIEQSTAVTA"
     gene            complement(3638..>4570)
                     /locus_tag="VFV20_02965"
     CDS             complement(3638..>4570)
                     /locus_tag="VFV20_02965"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU5287339.1"
                     /translation="DLTDIDIEIPAGRPDTEPRWDLQRGQEIDGTLVVIDLLGVGSRY
                     ETYRAWDRLLFCEVAAKVIRPHRVDDERALAGFERETTIGARLTHPNLVRVLRWSASP
                     PRPYLVMEFVDAATLGDHLNETGAVSVPEICLLGIRMASALHHMHANSTLHLDVKPEN
                     VTMGDPPKLLDLSLARTVSGPMRLRHTLGTRAYMAPEQCEHGLVTPQTDLFGLGATLY
                     EGASAMRPFPEGDDVAEERAARYPQLELDAPPLDELENVPRALAQIVMACLQRDPARR
                     PRSAIDVAVALHGVLEGFALKELFAWPKGLRVRA"
BASE COUNT          660 a         1628 c         1618 g          664 t
ORIGIN      
        1 tgttcgtcga gcgccgcgcg ggcctcacgc gcgggcgcca gatccggcac cgcgcgcagc
       61 aggcgcacga ggagccagtg catctcgacg caggagtgcc agtcgaagct cccgtagaac
      121 gcgggatgca gctcgcgcgg cggcgtgagc ggccccggtc cggtgatcca gtaggagaga
      181 tgggtcggat actcgcgacg cacgttggcg atcgcgacgc gcgcgtactc ggcggcgttc
      241 tcctcgagca ggcgccggcg cgcgctcgcg tccagcgtca ggcggtgacc gccgtcgact
      301 gctcgatcgc cgcgcggagt gcgaccgcgg agagccgctc ggcgatcgcc ttcgcctcgt
      361 cgcagaacgt ctgcgcctgg tcgcggcggc ccgtctcgcg cagcgagcgc gcgaggtcgg
      421 cgagcgcgac ggcgcgctcg tagtccagct tcagcgtcgc gaacgactgc accgcgtcga
      481 acaggagctt ggacgccttc gtgtgctcgc ccgccttctg cgcggccacg ccctcggcct
      541 gcaccagcag cgcccggccg cgcgcgtggc cgctgcgctc gacggcacgc tcgacgcgct
      601 ccgcgaacgc gggatcctcg atgcgccagc gggccgccgc gaggacggcg agcggaccga
      661 ggacgacgag gtcgacgccg cccttgggat acccggcgct gcggatcgcg cgatatgcgg
      721 actcggcctc acgcagtccg tacacggcgg tcaggaagtc acaggcggcg atgtcgaccg
      781 gcccggcctc gatctcgacc agccgctcgc ggagcgtctt ctcgtctgga agcgccatcg
      841 cggcggccgc ggcggccgcg atcgcgagcg cgttgtgtcc cttgacctgc ggcatgacgc
      901 gatcacggcg cgcggcggct tcgatggtgc ccgggaagtc acccgagagg aggcgcgcgt
      961 aggcctccac ctggagcgcc tgcgccgcga ggcgaggctt ctcggtctcg gcggcgatct
     1021 ccgatgcggc gaccgcgttc tcgagcacct cggggaagtt accgagcacg agtgccgcga
     1081 ccgcgaccat cgtgtgcgcg tcgatgagct cgtcgctgtc ctgcagcgac ttcgcgatcg
     1141 gcagacgacg ctggttctgc gcgtacgact cgcggaagtt ccccagctcg ccatacgcgt
     1201 agccgacggc gtcgaggcag aggctcacct cgcgcagcga gccgagatcc tccgcgatcg
     1261 cgagcgcctc cttcgccgtc gcgagcgacg cttcccagtc cgcgtctgtg ggcttcggac
     1321 ccttgcgcgc catgaacgcc ttcgcggcga ggagcagcac cttctcccgg ttgcgctcgc
     1381 cgtccacgat cgcgaggccc cgatcgatca ggccctgcag gtcgggatgc ttcgcgtccc
     1441 agcgggtgca gagctcggcc atgtgcgcga gcatcgtggc gacggcggga ccgttcgggt
     1501 cctgcgcctc gagcagcgac agcgcgcgct cgtacgcggc gagggcgccg tcgttctcgc
     1561 cgcgataccg ccggatgcgg gcgatgaggt agagcgcttc gaccgcctga tcggtcttgc
     1621 ccagcttctc gtagatctcg agcgcctgtt cggcgaaacg cagcgcttcg acataggcgt
     1681 ggatcgcggt cgcgcgctgc gcggccttcc acgagtacgc gaccgccttc tccggcacgt
     1741 ccgcgaggag gaagtgatgg gcgagcgccg gatagaactc gcgcagctcg gtctccgaca
     1801 gcaccttctc gtacgcggcg gcaacgcgcc cgtgcatctc ggagcggcgg cgcaccagca
     1861 gcgtgttgta cgcgacctcc tggacgacgg cgtgcttgaa ccggtagcgg ccctcgcgcc
     1921 gctcgcccgg tgccgcctcg agcacgaggt cggcggcgat gagctggtcc accgcgtcgg
     1981 cgagccggtt gccgccggcc tcacgcagca cccggtcgcc gaagcgctgg ccgatgaccg
     2041 acgcgagctg gatgcactcg cgtgcgttgg gcgggagccg gtcgatgcgt gcggcgacga
     2101 cggcatggag ggtggtcggg acctcgagca tcgacggacg gtcgcgcagc cgccattcac
     2161 cggtctcgtc cttctggacc gcaccggact ccaccaggga gcgcagcgac tcctcgatga
     2221 agaacgggtt gccgccggcg cgcgccacga tgcggtcgcg cagctcgtcg gggatccagt
     2281 cgaaggtcgc ctcgacgatc cgcgcggcct cttcgtcggg aagaggctcg aggaccagct
     2341 gcgtgaagtt cgtgcgtgag ggtcgcggct cgggggcacc gggccccatc cgctgcgcga
     2401 gcaggaagag gagcggaacg cgggaggcgc gcgacgccag gaaccacagc agatcgagcg
     2461 aggccgcgtc ggcgaagtgg agatcctcga gcacgtagag cagcggacgc tcgcgcgcga
     2521 gcgcggcgat gacgtcgtac acgacgatga acatcgagcg gcgccactcc tccgtgtcgc
     2581 tcgccggctc gggcgcgccc gcgaggtgca ggaactgggc gagcaccggc gccaccgcga
     2641 gcgggttcgc gaacccgagc gcggcgaggc gctccgccac cacctcctgc ccgttcggca
     2701 tcgccgtgtc caccttgagc tcgacgagca gcggctcgat gaagccggca tagctgcgct
     2761 ggttcacgcg cgagaacgtc cagcggagga cccgcggcgc ctccggtgcg ctcgccccgt
     2821 cctcggaggc ggtggcgagg ccgatgaact cggagaggag ccgcgtcttc ccgatgcccg
     2881 gctcgccggt gatcagcacg acctcggtgt gccccattcg cgacgactgg taggcgagat
     2941 cgagccgcga gagctccatc catcggccga cgagcggcgc ggccacgtcg atcatcggcc
     3001 gcgcgtcggt gcgctccccg gtgagcgcgt acacgaggac cggcttgtcc tttcccttga
     3061 gatcgacggg gccgacctcg cggaaggaga actcgcggtt ggtcaggcgg aacgtcgtct
     3121 gggacacgta gatctcggtg ggaccggccg cggcctggat gcgcgacgcg gtgttgaccg
     3181 tgtcacccat cacgccgaac tcggcgaccg agcgcaccga acccgcgacc accatcccgg
     3241 tgtggacccc gatgcgcagg ccgaactcga tgccgcggtc tttcttcacg cgcgcggcgt
     3301 ggtccgcgaa caccttctgc atcgccagcg cggtccggag cgcgcgctgt ggatcgtcct
     3361 catgcgccac cggcgccccg aagatcacga agatcgcgtc gccgatgaac ttctcgatcg
     3421 tgccgtcgtt gcggacggcc tcctcggcca ggtcctcgaa gatgcccgag acgaggacct
     3481 gcagctcctc gggatccatc tggtccgcca gctgcgtgaa gcccgcgaaa tcggcgaaga
     3541 gcaccgtgac cagccgccgt tcctgcccct gctgctgcac cgatgaaacg atagacgccg
     3601 gcccaatgtc gccgccgcta ccttcggcgg cgccccgtca cgcgcgcacg cgcaggccct
     3661 tcggccaggc gaagagctcc ttcagcgcga agccttccaa cacgccgtgc agggccaccg
     3721 cgacgtcgat cgccgaacgc ggcctgcgcg ccgggtcgcg ctgcaggcac gccatcacga
     3781 tctgcgcgag cgcgcgcggc acgttctcga gctcgtcgag cggtggcgcg tcgagctcga
     3841 gctgcggata gcgcgccgcc ctctcctccg cgacgtcgtc gccctcgggg aacggccgca
     3901 tcgccgatgc gccctcgtac agcgtcgcgc ccagcccgaa gaggtccgtc tgcggtgtca
     3961 ccaggccgtg ctcgcactgc tccggcgcca tgtaggcgcg cgtccccagg gtgtggcgca
     4021 gacgcatcgg tccggacacc gtccgtgcga ggctgaggtc gagcagcttc ggcgggtcgc
     4081 ccatcgtgac gttctcgggc ttcacgtcga gatgcagcgt gctgttcgcg tgcatgtgat
     4141 gcagcgcgga cgccatccga atgccgagca ggcagatctc cggcacgctc accgcgccgg
     4201 tctcgttcag gtggtccccg agggtcgcgg catcgacgaa ctccatgacc aggtacggac
     4261 gcggcgggct cgcgctccag cgcaggacgc gcacgagatt cggatgggtg agccgcgcgc
     4321 cgatcgtcgt ctctcgctcg aagcccgcga gcgctcgctc gtcgtcgacg cggtgcggcc
     4381 ggatcacctt ggccgcgacc tcgcagaaga ggagacggtc ccatgcgcga tacgtctcgt
     4441 accgcgagcc gaccccgagc aggtcgatca ccacgagtgt cccgtcgatc tcctggccac
     4501 gctggaggtc ccagcgcggc tcggtatccg ggcgccccgc ggggatctcg atgtcgatgt
     4561 ctgtgaggtc
//
LOCUS       DASTZR010000129         2299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1130441, whole genome shotgun sequence.
ACCESSION   DASTZR010000129 DASTZR010000000
VERSION     DASTZR010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130441"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..1230
                     /locus_tag="VFV20_02970"
     CDS             40..1230
                     /locus_tag="VFV20_02970"
                     /inference="COORDINATES: protein motif:HMM:NF013776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5287340.1"
                     /translation="MPGPFGRVAAPPPHFPVGGPVPAADLVGRETYIRRIAERLEDGN
                     HVLLAGPRRIGKTSIILEVTRRLRRRGALTAYVDCLGATDVRGLGERLIDALLENVSG
                     VERSFEQAKAIAAGMRPTVKVRYEHVELALDLAREKNAQRFFEGSLDLPRALAARTGK
                     RVVVVFDEFQAAGRLGPRVFDVMRARFQAQRGVSYAFLGSEEGILEELFSEKGHAFYR
                     FAVPIDLADAGGHRFGISPDDWLEYIRAKFAARKITIDDASVDRVLDATGGHPQDTMQ
                     ACAALYYLMRDAGQRSVGAEHVAVAYEQALRELERPFALHWTELGTQKYLQLVAKRIA
                     HGAVLYASESGIPRPEVLRALESLRARGLVTRLGRGRYEFVEPMFGEYVRRLDESAGI
                     VPSR"
     gene            1227..1427
                     /locus_tag="VFV20_02975"
     CDS             1227..1427
                     /locus_tag="VFV20_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287341.1"
                     /translation="MTTLELRPGASVRYSPAHSDEWREGELVKASVNGEEWLVKNRFG
                     RFWLHVNRLRPPEDGTQPDHDD"
     gene            complement(1424..1687)
                     /locus_tag="VFV20_02980"
     CDS             complement(1424..1687)
                     /locus_tag="VFV20_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006542148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU5287342.1"
                     /translation="MGLIVFLIVGLIAGFVARALVPGPDPMGWLGTMVLGIIGSFVGG
                     TLAALLFGGTLDLNAAGLIGSIIGAIIVLLIWRAMGGERTRVA"
     gene            complement(1764..1835)
                     /locus_tag="VFV20_02985"
     tRNA            complement(1764..1835)
                     /locus_tag="VFV20_02985"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1800..1802),aa:Arg,seq:ccg)
     gene            1873..>2299
                     /locus_tag="VFV20_02990"
     CDS             1873..>2299
                     /locus_tag="VFV20_02990"
                     /inference="COORDINATES: protein motif:HMM:NF013097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEU5287343.1"
                     /translation="MSERDKELSLVQHLAELRDRVMVAAIAVAITTAFSFLFTTDIIR
                     FLLVPSGVEKVVAFSPTESFTTYFRVALFAGLALAMPVVLYEVYAYIDPALYPAERRF
                     IRFVGLPVLLLFVAGMSFCYYVLLPRALPFLINFGGDVIE"
BASE COUNT          353 a          791 c          781 g          374 t
ORIGIN      
        1 cgcgtcacca attgatacat ctaacgtata tttagcgacg tgcccggacc attcggccgc
       61 gtcgccgctc ccccacccca cttcccggtg ggcggccccg tgccggcggc ggacctcgtc
      121 gggcgtgaga cgtacatccg ccgcatcgcg gagcgtctcg aggacgggaa ccacgttctc
      181 ctggccgggc cgcggcgcat cggcaagacc tcgatcatcc tcgaggtcac gcgccgtctg
      241 cgccggcgcg gcgcgctcac cgcgtatgtc gactgcctcg gcgcgaccga cgtccgcggc
      301 ctcggtgaac ggctgatcga cgcgctgctg gagaacgtgt ccggcgtcga gcgcagcttc
      361 gagcaggcga aggcgatcgc cgccggcatg cgaccgaccg tgaaggtgcg ctatgagcac
      421 gtcgagctcg cgctcgacct cgcgcgcgag aagaacgcgc agcgcttctt cgaggggtcg
      481 ctggaccttc cgcgcgcgct ggcggcgcgc accgggaagc gcgtcgtcgt cgtcttcgac
      541 gagtttcagg ccgcgggccg tctcggaccg cgcgtgttcg acgtcatgcg cgcgcgcttc
      601 caggcgcagc gcggcgtgtc gtacgcgttc ctcggcagcg aggagggcat cctggaggag
      661 ctcttcagcg agaaggggca cgccttctac cgcttcgccg tcccgatcga cctcgccgac
      721 gcgggcggac accgcttcgg catctcgccg gacgactggc tcgagtacat ccgcgccaag
      781 ttcgcggcgc gcaagatcac catcgacgac gcgagcgtcg accgcgtcct cgacgcgacc
      841 ggcgggcatc cgcaggacac catgcaggcc tgcgcggcgc tctactacct gatgcgcgac
      901 gccggacagc gatcggtggg agcggagcac gtcgccgtcg cgtacgagca ggccctgcgc
      961 gagctggagc gccccttcgc gctgcactgg acggagctcg ggacgcagaa gtacctgcag
     1021 ctggtagcga aacggatcgc gcacggtgcc gtgctctacg cgtccgagag cggcatcccg
     1081 cgcccggagg tactgcgcgc gctcgagtcg ctccgcgcgc gcggactggt gacacggctg
     1141 ggccgcggtc gctacgagtt cgtcgagccg atgttcggcg agtacgtgcg caggctcgac
     1201 gagagtgcgg gtatcgttcc ttcacgatga ccacgctgga actgcgaccc ggtgcgagcg
     1261 tccgctactc gccggcgcac tcggatgaat ggcgcgaagg cgagctcgtc aaggcttcgg
     1321 tgaacggcga ggagtggctg gtgaagaacc gcttcgggcg gttctggctg cacgtgaacc
     1381 ggctgcgccc gcccgaggac gggacgcagc cggatcacga cgactaagcg accctggtgc
     1441 gctcgccgcc catggcgcgc cagatcagga gcacgatgat cgcgccgatg atcgagccga
     1501 tgaggcccgc ggcgttcaga tcgagcgtcc cgccgaaaag cagcgcggcg agggtgccgc
     1561 cgacgaacga accgatgatc ccgaggacca tcgtcccgag ccagcccatc gggtcggggc
     1621 cgggcacgag cgcgcgagcg acgaagccgg cgatcaggcc gacgatgagg aaaacgatca
     1681 gacccacggt gtcacctctc cttgtatcgg atgtggtgga cgtgggtgca cgaacgatgc
     1741 ccgttcggct gaggaaggcg cagtaccccc gagcggactc gaaccgctgc ttcaggcttc
     1801 ggaggcctgc gctctatcca ctgagctacg ggggcgaacg tctcaagttt acctgcgcta
     1861 gcgtggccgc acgtgtctga gcgcgacaaa gagctctccc tcgtccagca tcttgccgag
     1921 cttcgcgacc gcgtcatggt cgccgccatc gcggtcgcca tcacgacggc gttctccttc
     1981 ctcttcacca ccgacatcat ccggttcctg ctggtcccct cgggggtcga gaaggtggtc
     2041 gcgttctcgc ccaccgagag ctttacgacc tacttccggg tggccctctt cgccggcctc
     2101 gcgctggcga tgccggtcgt cctgtacgag gtctacgcgt acatcgaccc ggccctgtat
     2161 ccggcggagc ggcgcttcat acgcttcgtg ggattgccgg tcctgctgct gttcgtggcc
     2221 ggcatgtcgt tctgctacta cgtgctgctg ccgcgcgcgc tcccgttcct catcaacttc
     2281 ggcggcgacg tgatcgaga
//
LOCUS       DASTZR010000130         2918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1026216, whole genome shotgun sequence.
ACCESSION   DASTZR010000130 DASTZR010000000
VERSION     DASTZR010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026216"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /gene="ribD"
                     /locus_tag="VFV20_02995"
     CDS             <1..937
                     /gene="ribD"
                     /locus_tag="VFV20_02995"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEU5287344.1"
                     /translation="HRAAGEAHAEVVALRAARERARGATLYSTLEPCAHQGRTPPCVD
                     AIREAGIVRVVSAMRDPDPRTDGKGFRLLRAATIEVTQGVLEEEARRLNEGFVSRLSR
                     GRPFVLVKLATTVDGRVAVKGRRYLSGKRALKEVHRLRDRYDAVLVGIGTVLADDPAL
                     TVREVRGRDPLRVVVDTEARTPVAAKVVRAKDPQRTVVCVARDADARRVNRLRETGVL
                     VTTLPRAEGGLDLAALMRWLADQGVNTVLCEAGPKIAGALVSAGLADRLMLITAPIAG
                     GDGPTALDGIGRSVALRDLRVRRLGEDVAIEGEVS"
     gene            938..1528
                     /locus_tag="VFV20_03000"
     CDS             938..1528
                     /locus_tag="VFV20_03000"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019434727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEU5287345.1"
                     /translation="MFTGIVEEIGTVKHMAPTGSGTRLEIACTQVMERLEVDDSVAVA
                     GTCLTVVARDDRSFAADVVPETLARTSLGSLTRGSRVNLEAAATPTTALGGHFVQGHV
                     DATTRLMTREPEGEGARLRFALPKDLARYVVQKGFITVDGVSLTVARLGKTFFEIALI
                     PHTARQTTLGTLRAGEPVNLEVDVLAKYVERIVRAR"
     gene            1548..2822
                     /locus_tag="VFV20_03005"
     CDS             1548..2822
                     /locus_tag="VFV20_03005"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011459820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEU5287346.1"
                     /translation="MKGTPRARRKVTPHPRIVSPEPPFATVDEAIADMAKGRMVIVVD
                     DEDRENEGDLTMAAELCTPEDVAFIRRYASGVVCVPMTAERLQQLDLPQMVARNEARL
                     GTAFTVTVDAREGITTGISAGDRARTIQVLADPKSVPADLVKPGHVFPLQAREGGVLV
                     RAGQTEAGVDLCRLAGLQPVAVICEITNPDGSMSRVPELKRFAKRHGMKMITVKDLIA
                     YRMAREQLVERVATASLPTRYGPFTVHGYRARFGNAEAEHVALVMGDVRDGKPVLVRV
                     HSECLTGDVFGSYRCDCGEQMDAALERITKEGRGVFLYLRQEGRGIGLMNKLRAYALQ
                     DQGLDTVEANEHLGFKADHREYGIGVQILMDLGVRRARIMTNNPQKASVSLYGFEVVE
                     RVPIEVPERPQNRAYLATKRAKMGHLLGIAKD"
BASE COUNT          444 a         1006 c         1063 g          405 t
ORIGIN      
        1 tcaccgtgcg gcgggtgagg cgcacgccga ggtcgtcgcg ctgcgcgcgg cgcgagagcg
       61 cgcacgtggc gcgacgctgt attcgacgct cgagccgtgc gcgcaccagg gccgcacgcc
      121 gccctgcgtc gacgcgatac gcgaagcggg gatcgtccgc gtcgtctcgg cgatgcgcga
      181 cccggatccc cgcacggacg gcaagggatt ccggctgctg cgggccgcga cgatcgaggt
      241 cacgcagggc gtgctcgagg aagaggctcg gcgcctcaac gaaggcttcg tgagccggct
      301 gtcgcgcggc cgcccgttcg tcctggtgaa gctcgcgacg acggtcgacg gccgagtggc
      361 ggtcaaagga cgccggtatc tctccggcaa gcgcgcgctc aaggaggtgc atcgcctgcg
      421 cgaccgctac gacgcggtgc tggtcggcat cgggacggtc ctcgccgacg atcccgcgct
      481 cacggtgcgc gaggtccgcg gtcgcgatcc gctgcgcgtc gtcgtggaca cggaagcgcg
      541 cacgccggtg gcggcgaagg tcgtgcgcgc gaaggatccg cagcgcaccg tcgtctgtgt
      601 ggcccgcgat gccgacgccc gtcgggtgaa ccgcctccgc gagaccggcg tgctcgtgac
      661 gacgctgccg cgcgccgagg gcggcctcga tctcgcggcg ctcatgcgct ggctcgccga
      721 ccagggcgtg aacactgtgc tctgcgaggc gggcccgaag atcgcgggcg cccttgtctc
      781 cgccggtctt gccgaccgcc tcatgctcat cacggcgccg atcgcgggcg gcgatggtcc
      841 gacggcgctc gacggcatcg ggagatcggt cgcgctccgc gacctccgcg tacgccggct
      901 gggcgaggac gtggcgatcg aaggcgaggt gtcataggtg ttcaccggca tcgtcgagga
      961 gatcggcacc gtgaagcaca tggcgccgac cgggtccggg acacgcctgg agatcgcgtg
     1021 cacgcaggtc atggagcggc tcgaggtcga tgacagcgtg gccgtcgcgg ggacgtgcct
     1081 cacggtcgtc gcgcgcgacg accgctcctt cgccgccgac gtcgtgccgg agacgctcgc
     1141 gcgcaccagc ctcgggtccc tcacgcgcgg atcgcgcgtg aacctcgagg ccgcggccac
     1201 gccgacgacg gcactgggcg gccacttcgt gcaggggcac gtcgacgcga cgacccggct
     1261 catgacccgg gagccggagg gcgaaggcgc acggctgcgc ttcgcgctgc cgaaggacct
     1321 ggcgcgctac gtcgtgcaga agggcttcat cacggtggac ggcgtgagcc tcaccgtggc
     1381 gcgcctcggc aagaccttct tcgagatcgc gctcatcccc cacaccgcgc ggcagaccac
     1441 cctcgggacg ctgcgcgccg gcgagcccgt gaacctcgag gtagacgtgc tggcgaagta
     1501 cgtcgagcgg atagtccgcg cgcggtagcg cacctagaat cgaaggcatg aagggcacgc
     1561 cacgcgcgcg gcgcaaggtc acgccacatc cgcgcatcgt gtctccggag ccgccgttcg
     1621 cgaccgtcga cgaggcgatc gccgatatgg cgaagggtcg catggtgatc gtggtggacg
     1681 acgaggaccg cgagaacgaa ggcgacctca cgatggcggc cgagctgtgc actccggagg
     1741 acgtcgcctt catccgccgc tacgcgagcg gcgtggtctg tgtgccgatg accgcggagc
     1801 ggctccagca gctggacctt ccacagatgg tcgcgcgcaa cgaggcacgg ctcggtacgg
     1861 cgttcacggt gaccgtcgac gcgcgcgagg gcatcaccac cggcatcagc gccggcgatc
     1921 gcgcgcgcac catccaggtg ctcgccgacc cgaagagcgt ccccgccgac ctcgtgaagc
     1981 ccggacatgt cttccccctc caggcgcgcg agggcggggt cctcgtgcgc gccggacaga
     2041 ccgaggcggg cgtcgatctg tgccggctcg cgggcctgca gccggtcgcg gtcatctgcg
     2101 agatcacgaa ccccgacgga tcgatgtcgc gcgtgccgga gctgaagcgg ttcgccaagc
     2161 ggcacggcat gaagatgatc acggtgaagg acctcatcgc gtaccgcatg gcgcgcgagc
     2221 agctcgtcga gcgtgtggcg acggcgtcgc tccccacgcg ctacggcccc ttcaccgtgc
     2281 acggctaccg cgcccgcttc ggcaacgccg aggccgagca tgtcgcgctc gtcatgggtg
     2341 acgtgcgtga cggcaagccg gtgctggtgc gcgtgcactc ggagtgcctc accggcgacg
     2401 tgttcggctc gtaccgctgc gattgcggcg agcagatgga cgcggccctc gagcgcatca
     2461 ccaaggaggg tcggggcgtc ttcctctacc tacggcagga gggtcgcggc atcggtctca
     2521 tgaacaagct gcgcgcctac gcgctgcagg accagggact cgacaccgtc gaggcgaacg
     2581 agcacctcgg cttcaaggcg gaccatcgcg agtacggcat cggcgtccag atcctcatgg
     2641 acctgggagt gcgccgggcg cggatcatga cgaacaatcc gcagaaggcc tcggtgtccc
     2701 tgtacgggtt cgaggtggtc gagcgtgtcc ccatcgaggt gcccgagcgg ccgcagaacc
     2761 gcgcgtatct ggcgaccaag cgcgcgaaga tgggacacct gctcgggatc gcgaaggact
     2821 agcgcctagc gcgcggcctg ccgccacgtc gtcgcgagct ggaacagctg gccctctgtc
     2881 ccgccaccga gatcccggcc gttggtggtg acctcacg
//
LOCUS       DASTZR010000131         2246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_553981, whole genome shotgun sequence.
ACCESSION   DASTZR010000131 DASTZR010000000
VERSION     DASTZR010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553981"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VFV20_03010"
     CDS             complement(<1..271)
                     /locus_tag="VFV20_03010"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU5287347.1"
                     /translation="MKKRCRWAAREPLLSYHDVEWGTPLHDEHGLFEFLVLEGAQAGL
                     SWETILRKRDAYRRAFDRFDPKKVARYGARDVARLLRDAGIVRNRA"
     gene            complement(268..1008)
                     /locus_tag="VFV20_03015"
     CDS             complement(268..1008)
                     /locus_tag="VFV20_03015"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU5287348.1"
                     /translation="MTAPRAVVFDLGGTLVHWPDWEDGAAAKWSFAYDALLRAGGDRP
                     FPARETFIAAMREAEKAHWVRVDRDHWSGPPTGLVSDGFRRLDLSVEDDVLIATMDGY
                     AKAVAGWSAVFPDARDTLQLLRERGYRVGLLSNTWWAAEWHNADLAAHGLAHLLDELV
                     YTSDLPHSKPHPSVFAEVAARLEVDPASAVMIGDRQIDDVAGAKAVGMRGIWRRNDSG
                     FPTSPVTPDATVDRLAELPALLRSWGGA"
     gene            complement(1005..>2246)
                     /gene="hutH"
                     /locus_tag="VFV20_03020"
     CDS             complement(1005..>2246)
                     /gene="hutH"
                     /locus_tag="VFV20_03020"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEU5287349.1"
                     /translation="TRAILLLRANTLAAGRSGVRVEVIELLLGMLDSGVHPVIPMHGS
                     VGASGDLAPLAHAALVLIGEGEAIVGGKRMPGAEALKKAGLRPIELAPKEGVALINGT
                     QVMTAIGCLALHDAEVLARSADVIGALSAEALRATDAAWDEALIAARAHPGQIAVAAN
                     LRALMSGSPNVESHRTGDPRVQDPYSVRCMPQVHGASRDALAYARRVLEIEIDAVTDN
                     PLVFAEERRVVSGGNFHGQPVAIALDAATIAVAELADISEARIDRMTNGHTSGLPPFL
                     ASAPGIDSGFMVAQYTAAALVTENRLRSFPASVESLPTSAGMEDHVSMGTHAAHKLTA
                     VVRNTRDVLAIEALCAAQGLDLLGARVAPPAEEARRVIREHVAALAHDRVLAPDVALL
                     SDVIGRGLLVAAVRVRLPEIE"
BASE COUNT          315 a          822 c          808 g          301 t
ORIGIN      
        1 tcgcgcggtt gcgcacgatg cccgcatcgc gcagcagacg cgcgacgtcg cgggctccgt
       61 accgcgcgac cttcttcggg tcgaagcggt cgaacgccct ccggtacgcg tcgcgcttgc
      121 gaaggatcgt ctcccaggag agccccgcct gcgcgccctc gaggacgagg aactcgaaga
      181 gcccgtgctc gtcgtggagc ggcgtgcccc actccacgtc gtgataggaa aggagcggct
      241 cgcgcgccgc ccagcggcag cgcttcttca cgcccctccc cacgagcgca gcagcgcggg
      301 cagctcggcg agccgatcga cggtcgcgtc gggcgtcacc ggcgaggtgg ggaagccgga
      361 gtcgttcctc cgccagatcc cccgcatccc tacggccttc gccccggcga cgtcgtcgat
      421 ctggcggtcg ccgatcatga ccgcggacgc cggatcgacc tcgaggcgag cggcgacctc
      481 cgcgaagacc gatggatgcg gcttgctgtg cggcaggtcc gacgtgtaga cgagctcgtc
      541 caggaggtgc gcgagcccgt gcgccgcgag atcggcgttg tgccactccg cggcccacca
      601 ggtgttcgac aggaggccaa cgcgataccc gcgctcccgc agcagctgca gcgtgtcacg
      661 cgcgtccggg aagaccgccg accacccggc cacggccttc gcgtagccgt ccatcgtcgc
      721 gatgagcacg tcgtcctcga ccgagaggtc gagccggcgg aagccatccg agacgagccc
      781 ggtcggcggg ccgctccaat ggtcgcggtc gacgcgcacc cagtgcgcct tttcggcctc
      841 gcgcatcgcc gcgatgaagg tctcgcgcgc cggaaacggg cggtcaccgc cggcgcgaag
      901 gagcgcgtcg taggcgaacg accacttggc cgccgcgcca tcctcccagt ccggccagtg
      961 cacgagggtc ccgcccaggt cgaagacgac cgcgcgcggc gccgtcactc gatctcgggg
     1021 agacgcacgc gtacggcggc gacgagcagg ccgcggccga tgacgtcgga caggagcgcg
     1081 acgtccggcg cgagcacgcg gtcgtgcgcg agcgcggcga catgctcgcg gatcacgcgc
     1141 cgcgcctcct cggccggcgg tgcgacgcgc gcgcccagca gatcgagacc ttgcgccgcg
     1201 cacagcgcct cgatcgcgag cacgtcgcgc gtgttgcgca ccacggcggt gagcttgtgc
     1261 gccgcatgcg tccccatgct cacgtggtcc tccatgccgg ccgaggtcgg gagcgactcg
     1321 accgaggccg ggaacgagcg caggcggttc tcggtcacga gggcggccgc ggtgtactgc
     1381 gccaccatga atccggagtc gatcccgggc gcggacgcga ggaacggtgg cagcccgctg
     1441 gtgtgcccgt tcgtcatgcg gtcgatgcgc gcctcgctga tgtccgcgag ctcagcgacc
     1501 gcgatcgtgg cggcgtcgag agcgatcgcg accggctggc cgtggaagtt gccgccgctc
     1561 acgacgcggc gctcctccgc gaagaccagc gggttgtcgg tcaccgcgtc gatctcgatc
     1621 tcgagcacgc ggcgcgcgta tgcgagcgcg tcgcgcgacg caccgtgcac ctgcggcatg
     1681 caccggaccg aatagggatc ctgcacgcgc gggtctccgg tgcggtgcga ctcgacgttc
     1741 gggctcccgc tcatgagggc gcggaggttc gcggcgacgg cgatctgccc gggatgcgcc
     1801 cgcgccgcga tgagcgcctc gtcccacgcc gcgtcggtcg cgcggagcgc ctccgccgag
     1861 agcgcgccga tgacgtccgc gctcctcgcg aggacctcgg cgtcgtgcag cgcgaggcag
     1921 ccgatcgcgg tcatcacctg ggtcccgttg atgagggcga cgccttcctt tggcgccagc
     1981 tcgatcggcc tcagacctgc cttcttcagc gcctcggcgc cgggcatgcg tttcccaccg
     2041 acgatcgcct cgccctcgcc gatgagcacg agcgccgcgt gcgcgagcgg cgcgagatcg
     2101 ccggacgcgc cgacgctgcc gtgcatcggg atgaccgggt ggacgccgct gtcgagcatg
     2161 ccgagcagca gctcgatgac ctcgacgcgg acacccgaac gtcccgcggc gagcgtgttc
     2221 gcgcgcagca ggaggatcgc gcgcgt
//
LOCUS       DASTZR010000132         3630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_841981, whole genome shotgun sequence.
ACCESSION   DASTZR010000132 DASTZR010000000
VERSION     DASTZR010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841981"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1345
                     /locus_tag="VFV20_03025"
     CDS             <1..1345
                     /locus_tag="VFV20_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5287350.1"
                     /translation="DSRHVDAMDRDMTTVAMPPGATPPAPPARAPGPEPAAPLAPGSL
                     EETGLSSTFVVDMVVKLLYQRGQSSATEIADALCLPLPKIVQPILDFLKNEHFTEVKG
                     ASSLAPGTYVYSLAARGAERAREAMERNAYIGAAPVPLEAYVARVRAQAAGDLHVSMD
                     QLRKALAHLVLPERTLRQLGPAVNSGRSIFLFGPPGTGKSSIAHAIATLMQGGVLLPH
                     AIVVGQEVIRLFDASRHRPLMQLDARFDRRWVPVTRPFVEVGGELTLEDLDLSADPTT
                     KVHEAPFQMKASGGVLLIDDFGRQRMSPDQLLNRWIVPLDRGVDFLTTRDGRKIEVPF
                     DVLLVFSTNRPPSSLVDEAFLRRIQFKIEISAPNEQEFAEILRRVCEGQGITFYPQAA
                     YSIAQYARERNIALRSCHPRDLMQHLGAAARFLGRRPELTPELVRLACETYFVAL"
     gene            1419..2141
                     /locus_tag="VFV20_03030"
     CDS             1419..2141
                     /locus_tag="VFV20_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287351.1"
                     /translation="MATLLGRRFGQQIAGWLIAFPWTSAPVSFFLALDHGPEFAATAA
                     KGSVAIAVGEVAFAVGYSRVKRGWLLSLLAGSVGYVLAGVAFGFVDLPVLPLALVVGV
                     LVILGGRLLPPPAPTTRVAHVVPRWDLPARVLLTTALVLGITLVAPRLGAYGSAIVAS
                     FPLYGCVLAVFAERHGRHADAVDVMRGMVSGLFGYMSFFIVIALALVPLGTVLAFALA
                     VLTVLVVQGITLAALHGRAPWQ"
     gene            complement(2158..2937)
                     /locus_tag="VFV20_03035"
     CDS             complement(2158..2937)
                     /locus_tag="VFV20_03035"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5287352.1"
                     /translation="MKLEGKTIAITGGASGIGLATAQRVVADGGNAVVIDLNAGAVRD
                     AAQLLGNAHALGMAADVTDAAAIGHALDAAASHFGRLDGLVTSAGIRQASARVTDLSA
                     EVWERILRVDLGGTFLTCQAAGRIFAKSGGSGSIVNVASLSGHSPRVGQAAYCAAKAG
                     VIALTKVLSLELASQHVRVNCVCPGTTITPFNSEALKREGEQGIQARIEGDTKQYRPG
                     IPLRQLAEASDIASSIAFLLSDDARHITGVELFVDGGESVF"
     gene            complement(2951..3253)
                     /locus_tag="VFV20_03040"
     CDS             complement(2951..3253)
                     /locus_tag="VFV20_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287353.1"
                     /translation="MIAGVTLLARRDPVLAGWVSVFPTATSLALVWLAIDRTPEARMA
                     DFTGGVIAGLLPSVSILIAFAVLLRLNVPFSVTAVAVVGLWLVLTLVVRQLGFFVT"
     gene            complement(3289..>3630)
                     /locus_tag="VFV20_03045"
     CDS             complement(3289..>3630)
                     /locus_tag="VFV20_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEU5287354.1"
                     /translation="ERLTPDHLRAAGASPDTPVVGMEKRQEFWRYMAQNHDVLDVMAA
                     RQDILDGGEELVTTHANIGAIVLECTNMVPYARALRAHVKLPVFDIRAFISWFQSGLD
                     PHGFGLPGDPL"
BASE COUNT          520 a         1281 c         1248 g          581 t
ORIGIN      
        1 agactcccgc cacgttgacg ccatggacag ggatatgacc acggtcgcga tgccgccggg
       61 cgccacgccc ccggcgccgc ccgcgcgtgc gcccggtccg gaaccggcgg cgccgctcgc
      121 gcccgggtcg ctcgaggaaa cggggctcag ctcgaccttc gtcgtcgaca tggtcgtgaa
      181 gctgctctac cagcgcgggc agtcctcggc gaccgagatc gcggacgcgc tgtgcctgcc
      241 gctcccgaag atcgtgcagc cgatcctcga cttcctgaag aacgagcatt tcaccgaggt
      301 gaagggcgcc tccagcctcg cgcccggcac gtacgtgtac agcctcgcag cgaggggagc
      361 cgagcgcgcg cgcgaggcga tggagcggaa cgcgtacatc ggcgcggcac cggtcccgct
      421 cgaggcgtac gtcgcgcgcg tgcgcgcgca ggccgccggc gacctgcacg tgtcgatgga
      481 tcagctgcgc aaggcgctcg cgcacctcgt gctgcccgag cggacgctcc gccagctcgg
      541 ccccgcggtg aacagcgggc ggtccatctt cctgttcggg ccgcccggga cggggaagtc
      601 ctcgatcgcg cacgccatcg ccaccctcat gcagggcggc gtcctcctgc cgcacgccat
      661 cgtcgtcggg caggaggtca tccggctctt cgacgcctcg cgccaccgtc cgctgatgca
      721 gctcgacgcc cgtttcgacc ggcgctgggt gccggtgaca cggccgttcg tcgaggtcgg
      781 tggcgagctc accctcgagg acctcgatct cagcgccgac cccacgacga aggtgcacga
      841 ggcgccgttc cagatgaagg cgagcggtgg cgtgctgctc atcgacgact tcggccggca
      901 gcgcatgtcg ccggaccagc tcctgaaccg ctggatcgtg ccgctcgatc gcggcgtcga
      961 cttcctcacc acgcgtgacg gccgcaagat cgaggtgccc ttcgacgtgc tgctcgtgtt
     1021 ctccaccaac cgtccgccgt cctcgctcgt ggacgaggcc ttcctgcgcc gcatccagtt
     1081 caagatcgag atcagcgccc cgaacgagca ggagttcgcc gagatcctgc ggcgcgtgtg
     1141 cgaggggcag gggatcacct tctacccgca ggccgcgtac tcgatcgccc agtacgcgcg
     1201 cgagcgcaac atcgcgctcc gctcgtgcca tccgcgcgac ctcatgcagc acctcggcgc
     1261 ggcggcgcgt tttctcggcc ggcggccgga gctgacgccg gagctggtcc gccttgcctg
     1321 cgagacgtac ttcgtagcgc tctgacatat tcgcgcgcta ggctgcgcgc atcgacacgc
     1381 tccttcccaa gctcgtcttc acgcctgccc tcatcacgtt ggcgacgctg ctcggccgcc
     1441 gattcgggca gcagatcgcg ggctggctca tcgccttccc ttggacatcg gcgcctgtct
     1501 cgttcttcct cgcgctcgat cacggccctg aattcgcggc gaccgcggcg aaggggtcgg
     1561 tcgccatcgc ggtgggcgag gtcgcattcg ccgtcggcta ctcgcgggtg aagcgcggtt
     1621 ggctgctgtc gcttctcgcc gggagcgttg gctacgtgct cgccggcgtc gcgtttggct
     1681 tcgtcgacct cccggtcctt ccgctcgcgc tcgtcgtcgg cgtgctggtg atccttgggg
     1741 gacggctcct gcctccgccg gcaccgacca cacgcgtcgc gcacgtcgtg ccgcggtggg
     1801 atctcccggc ccgcgtcctc ctcaccaccg cgctcgtgct cggcatcact ctcgtcgcgc
     1861 cgaggctcgg cgcctacggc agcgcgatcg tcgcgagctt tccgctctac ggctgcgtgc
     1921 tggcagtgtt cgcggagcgg cacgggaggc acgccgacgc ggtcgacgtg atgcgcggca
     1981 tggtcagcgg cctgtttggc tacatgtcgt tcttcatcgt catcgcgctc gctctcgtgc
     2041 cgctcggcac cgtcctcgcc ttcgcgctcg ccgtcctcac cgtcctcgtc gtgcagggga
     2101 tcacgctcgc cgcgctgcac ggccgtgcac catggcagtg aacggcggct gaccggacta
     2161 gaagacggat tcaccgccgt cgacgaagag ctcgacgccg gtgatgtgac gcgcgtcgtc
     2221 ggagagcagg aacgcgatcg atgatgcaat gtcgctcgcc tcggcgagct ggcggagcgg
     2281 gatcccgggc cgatactgct tcgtgtcgcc ttcgatgcgt gcctggatac cctgctcgcc
     2341 ctcacgcttc agcgcctcgc tgttgaacgg cgtgatcgtc gtcccggggc aaacgcagtt
     2401 cacgcgcacg tgctgtgacg cgagctcgag cgagaggacc ttcgtgagcg cgatcacgcc
     2461 tgccttcgcc gcgcagtacg cggcctgtcc gacgcgcggc gagtgccctg agagagaagc
     2521 gacgttcacg atcgagccgc tgccgcccga cttcgcaaag atgcgacccg cagcctggca
     2581 ggtcaggaac gtgcccccga ggtcgacgcg gaggatgcgc tcccacacct cagccgagag
     2641 gtcggtcacg cgcgccgacg cttgacggat gccggcggac gtgacgagcc cgtcgagccg
     2701 cccgaaatgc gacgccgcgg cgtcgagcgc gtgaccgatc gcggcggcgt ccgtgacgtc
     2761 ggcggccatg ccgagagcgt gcgcgttccc gagcagctgc gccgcatcgc gcacggcgcc
     2821 ggcattgagg tcgatgacga cggcgttgcc gccgtcggcg acgacccgct gcgcggtcgc
     2881 gaggccgatg ccgctcgcgc ctccggtgat cgcgatggtc ttgccttcga gcttcatctg
     2941 gttgcccctc tcatgtgacg aagaagccga gctgacggac gacgagcgtg aggacgagcc
     3001 aaaggccgac gaccgcgacg gcggtgacgc tgaacggcac gttcaagcgc aggagtacgg
     3061 cgaacgcgat gaggatcgag acgctgggca gcaggcctgc gatgacgccg cccgtgaagt
     3121 ccgccatccg cgcctccggc gtgcggtcga tcgcgagcca gacgagcgcg agcgaggtgg
     3181 ccgtcgggaa gacggagacc cagccggcga gcacgggatc gcggcgcgcg agcagcgtga
     3241 ccccggcaat cacgaggccg gtgagggacg cgcggaaggc gagctcgatc agagagggtc
     3301 tcccggcaat ccgaatccgt gcgggtcgag gcctgactgg aaccagctga tgaacgcacg
     3361 aatgtcgaag accgggagct tcacgtgcgc gcggagtgcc cgcgcgtagg ggaccatgtt
     3421 cgtgcactcg agcacgatgg cgccgatgtt ggcgtgtgtg gtcacaagct cctcgccgcc
     3481 gtcgaggatg tcctgccgtg cggccatgac atcgaggacg tcatggttct gtgccatata
     3541 acgccagaac tcctggcgct tttccatacc gacgacgggc gtgtcgggcg acgcgcccgc
     3601 ggcgcgcagg tggtccggcg tcaggcgctc
//
LOCUS       DASTZR010000133         3284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_554039, whole genome shotgun sequence.
ACCESSION   DASTZR010000133 DASTZR010000000
VERSION     DASTZR010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554039"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VFV20_03050"
     CDS             complement(<1..232)
                     /locus_tag="VFV20_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287355.1"
                     /translation="MSVISAEAIVAPLAPDELRWRCDPASLGFRTTSDIAPLDTVVGQ
                     DRGLAAIDLALDIDDPEYNVFVAGPSGSGRNSA"
     gene            complement(252..452)
                     /locus_tag="VFV20_03055"
     CDS             complement(252..452)
                     /locus_tag="VFV20_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287356.1"
                     /translation="MSLRMERCTECGAPLTAGVCGNCAPTGRPVDKGERRLEAGAAAH
                     EKEQWDLQRVNRKVHQRARRAT"
     gene            complement(449..1126)
                     /locus_tag="VFV20_03060"
     CDS             complement(449..1126)
                     /locus_tag="VFV20_03060"
                     /inference="COORDINATES: protein motif:HMM:NF039189.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HEU5287357.1"
                     /translation="MIVGVFAMTAVFGVIALTLLLIFRKDLVGVDVSARSLLRLYLYI
                     ASLAGLLVFVIGIASLLDWAAGAAFGVQAVYGRAPSVWPSFMDYSNFADQSAMRHDDA
                     LLQGLTLTVFGALFWGGHAFARRSLGPDERSSVLRRAYNVLCTFVFGVGTVVMLPVGI
                     YQLLSYVLVTPAADVFKSGFGDSLAGGIASVPVWLVYLTRVVRAAPREARMPIPSRSA
                     PVRTGLV"
     gene            1291..1941
                     /locus_tag="VFV20_03065"
     CDS             1291..1941
                     /locus_tag="VFV20_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004979745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEU5287358.1"
                     /translation="MDTFRDRADAGRRLADLLGSYAEDRPIVLGLPRGGVVTAAEIAR
                     ALHAPLDVLVVRKLGAPAEPEFGVGAIAPGEVRIVDRASMRIAGMDEDDLRRVTRDET
                     AELERRERRYRGGRAPLDVRGRTVILVDDGIATGVTARAAISATRRAGATRIILAVGV
                     CAPDTAEALRAEVDDLVCVLVPEELAAVSLYFDDFRPVDDDDVVAALALGSVAVPV"
     gene            complement(1970..>3284)
                     /locus_tag="VFV20_03070"
     CDS             complement(1970..>3284)
                     /locus_tag="VFV20_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5287359.1"
                     /translation="TPFNSEAVVFNFDRARLTSFPFRNTRAIADDYDYYQSMWGGFDD
                     ASLLLKVEAFDRLTVRFTTRTPFGPFLPTIAMATFGMVSPASIKADRDGWMLATSSSA
                     AGTGPFMFTPGSWVKGDEIVLVRNPSYWRTDDRGQQLPYVDRVRLRTITDPGFRVAAL
                     RGGQVDAIRDFSPADMPLLKSDPKVTLLDRSPNNIGYLRFNTTHPPLDKPEVRKALAM
                     AVDRGALIGSVFTGFASPASQFLVPGMLGYDDSVREFQPFDVEGARRLLIAANAAQFE
                     LDLWYMDVARPYFPDPRRAAETIATDLRRLGITVVLRSIDFASYRDRAKRNQLQAWLL
                     GWTGDNGDPDNFLCVLFCNTTQNGAWSDQSAQQAVGLLHDAATETEPARRADLYRRAS
                     RVVQKSVPAVPIAHADVPVAASTFVSGYVPHPKGSESFALVRIAR"
BASE COUNT          531 a         1128 c         1168 g          457 t
ORIGIN      
        1 ctgccgagtt ccgtccgctg ccgctcggac cggcgacgaa gacgttgtac tcggggtcgt
       61 cgatgtcgag ggccagatcg atcgcggcga gcccccggtc ctgacccacg accgtgtcga
      121 gcggtgcgat gtcgctcgtg gtccggaagc cgaggctcgc gggatcgcac cgccagcgca
      181 gctcgtcggg tgcgagcggt gcgacgatcg cctccgccga gatgacgctc atcgctcccc
      241 ctggacgccg atcacgtcgc acggcgcgcg cgctggtgca ccttccggtt cacgcgctgg
      301 agatcccact gctccttctc gtgggccgcg gcgcccgcct cgagacgacg ctctcccttg
      361 tcgacgggac gtccggtggg cgcgcagttg ccgcagacgc cggcggtgag cggtgcaccg
      421 cactcggtgc accgctccat ccggaggctc atacgagacc ggtccggacg ggagcggacc
      481 gagatgggat cggcatccgc gcctcacgcg gcgcggcacg cacgacgcgc gtgaggtaca
      541 cgagccacac gggcacgctc gcgatcccgc ccgcgagcga gtcgccgaag ccggacttga
      601 acacgtcggc cgccggagtc accagcacgt acgacagcag ctggtagatg ccgaccggca
      661 gcatcacgac cgtacccacg ccgaagacga acgtgcagag gacgttgtac gcgcggcgca
      721 ggaccgagga ccgctcgtcc ggcccgagcg atcgtcgcgc gaatgcgtgt cctccccaga
      781 acagggcgcc gaagaccgtg agcgtcagac cttgcagcag cgcgtcgtcg tggcgcatcg
      841 cggattgatc cgcgaagttc gagtagtcca tgaacgacgg ccagaccgag ggtgcacgcc
      901 cgtagaccgc ctggacgccg aacgccgcgc cggcggccca gtccaggagc gacgcgatcc
      961 cgatcacgaa cacgagcaga cccgcgagcg aggcgatgta caggtagagc cggagcaggc
     1021 ttcgcgccga gacgtccacg ccgacgaggt ccttccggaa gatgaggagc agggtgaggg
     1081 cgatgacacc gaagacggcg gtcatcgcga aaacgccgac gatcatggca gtcctcctgc
     1141 acccagactc ccccagacca ccacgtggtc ggtagagcca aaggaagcac gccggctggc
     1201 ggcgcgtcgg ggagttgtag gggaagaccg cgcggcgatc gcgccgggcg cccgtctccg
     1261 cacgaggacc aggcaaggca gcatccgccc gtggatacgt tccgcgatcg tgccgacgcg
     1321 ggccgccgcc tggccgacct cctcggctcg tacgcggagg accgtccgat cgtgctcggc
     1381 ctgccgcgcg ggggcgtggt cacggcggcc gagatcgctc gcgcgctgca cgcgccgctc
     1441 gacgtcctcg tcgtgcgcaa gctcggcgca ccggcggagc cggagttcgg cgtcggcgcc
     1501 atcgcccccg gcgaggtgcg gatcgtcgat cgcgcgtcga tgcgcatcgc cgggatggac
     1561 gaggacgatc tgcggcgcgt gacgcgtgac gagacggccg agctcgagcg gcgagagcgc
     1621 cgctaccggg gcggacgcgc tccgctcgac gttcgaggcc gcaccgtgat cctcgtcgac
     1681 gacggcatcg cgacgggcgt caccgcccgc gcggccatct ccgcgacgcg gagggccggc
     1741 gcgacgcgga tcatcctcgc agtcggcgtc tgcgcgcccg acacggcgga ggcgctgcgc
     1801 gccgaggtcg acgacctcgt gtgcgtcctc gttcccgaag agctcgccgc cgtgagcctg
     1861 tatttcgacg acttccgtcc ggtcgatgac gacgacgtcg tcgcggcgct cgcactggga
     1921 tccgtggcgg tgccggtgta gcgcccgacc ggccacggga gtggccgatc tagcgcgcga
     1981 tccgcaccag ggcgaacgac tcgctgccct tcggatgcgg aacgtacccg ctcacgaagg
     2041 tggacgcggc cacggggacg tccgcgtgcg cgatggggac cgccggaacg ctcttctgca
     2101 cgacgcgcga cgcccgccgg tacagatccg cgcgtcgcgc cggctccgtc tcggtggccg
     2161 cgtcgtgaag caggcctacc gcctgctgcg cgctctgatc tgaccacgca ccgttctgcg
     2221 tcgtattgca gaacaggacg cacaggaagt tgtcggggtc accgttatcc ccggtccatc
     2281 ccaggagcca cgcctggagc tgattgcgct tcgcgcggtc gcggtacgag gcaaagtcga
     2341 tgctccgcag caccaccgtg atcccgagcc ggcgcaggtc tgtcgcgatc gtctccgcag
     2401 cgcgcctggg atcggggaag tacggccgcg ccacgtccat gtaccaaagg tcgagctcga
     2461 actgggcagc gttcgccgcg atcagcaggc gccgtgctcc ctcgacgtcg aatggctgga
     2521 actcgcgcac gctgtcgtcg tagccgagca tgcccgggac gaggaactgc gaggcgggac
     2581 tcgcgaaccc ggtgaagacg gagccgatca gtgcaccacg gtcgaccgcc atcgcgagcg
     2641 ccttgcgcac ctccggcttg tcgagcggcg ggtgtgtcgt gttgaagcga aggtagccga
     2701 tgttgttcgg cgaccgatcg agcagcgtga ccttgggatc cgacttgagg agcggcatgt
     2761 cggccggcga gaagtcgcgg atcgcgtcca cctgcccgcc ccgcaaggcg gcgacgcgga
     2821 atcccgggtc ggtgatcgtc cgcagccgga cgcgatcgac gtacggcagc tgctggccgc
     2881 gatcgtcggt gcgccagtag ctcggattgc gcacgaggac gatctcgtct cccttgaccc
     2941 acgagccggg cgtgaacatg aacggtccgg tgccggcggc ggagctcgag gtcgcgagca
     3001 tccagccgtc gcgatcggcc ttgatcgaag cggggctgac catgccgaac gtcgccatcg
     3061 cgatcgtcgg caggaacggc ccgaacggcg tccgcgtcgt gaagcgcacc gtcagccggt
     3121 cgaacgcctc caccttcagg aggagcgacg cgtcatcgaa cccaccccac atcgactggt
     3181 agtagtcgta gtcatcggcg attgccctcg tgttgcggaa tgggaaggac gtgagccggg
     3241 cgcggtcgaa gttgaagacg acggcctcgg agttgaaggg ggtg
//
LOCUS       DASTZR010000134         2006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_764325, whole genome shotgun sequence.
ACCESSION   DASTZR010000134 DASTZR010000000
VERSION     DASTZR010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764325"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..1079
                     /locus_tag="VFV20_03075"
     CDS             54..1079
                     /locus_tag="VFV20_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU5287360.1"
                     /translation="MTPRRTIHDVARLAGVHPSTVSRALSRADLPVRPETRRKVLAAA
                     RRLEYRPSPLARGLRLQRTKTLGMLVPDITNPFFPPIIRGAEEAARERGYELVLSNTD
                     DSPDRERASLGLMRERQADGLLIATSRMADATVTGVRRDRLPFVLVNRGSRVTADHSV
                     EVDNERAAETVVAHLASLGHRRIAHIAGPLSTTTGAERAAGYRAAMTAHGLGAHVLVV
                     EASAYSEEAGRDAAGKVLGADVTAIFAANDLIAIGVLLALREMGRRVPADVSLVGVND
                     IPLAGLLDPPLTTMRVPQREMGGIAARMLIALLEGEPVTRRHVVLETSLVVRGSTAAP
                     AGRGRMS"
     gene            1085..1852
                     /locus_tag="VFV20_03080"
     CDS             1085..1852
                     /locus_tag="VFV20_03080"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5287361.1"
                     /translation="MAGIRISVESATKHFVTGDVTVFDGLDLQVREGEALCILGPSGC
                     GKTTLLRCMHGLATLNSGRIVIDGSTVSTPRRNVAMVFQHFGLFPWKRVRENVAYGLE
                     LAGASRATIAERVERYIDMVGLRGFEDAYPYALSGGMQQRVGLARALAVEPEVLLMDE
                     PFGSLDALTREVLQDELLRIYQERPRTMVFITHSIEEAIALGDRVVVLSARPSRVREI
                     ISVDIPRPRTVESVIAHPHFVELRDRCWRLLREKATA"
BASE COUNT          273 a          728 c          712 g          293 t
ORIGIN      
        1 gggcatagcc tcggactcag gttgcgcaaa cgttttccga ggctcaacgc cgcatgacgc
       61 cgcgtcgcac gatccacgac gtcgcgcgcc tggcgggcgt gcacccgtcc acggtgtccc
      121 gggcgctctc gcgggcggac ctgccggtcc ggcccgagac gcgccgcaag gtcctcgccg
      181 cggcccggcg tctcgagtac cggccgtcgc cactggcgcg cggcctgcgg ctccagcgca
      241 ccaagacgct cgggatgctc gtccccgaca tcaccaatcc gttctttccg ccgatcatcc
      301 gcggcgccga ggaggccgcg cgcgagcgcg gctatgagct ggtgctgtcc aacaccgacg
      361 actcgccgga ccgggagcgc gcgtcgctcg ggctgatgcg cgagcggcag gcggacggcc
      421 tgctcatcgc gacgagccgg atggccgacg cgaccgtcac cggcgtccgg cgcgatcggc
      481 tgccgttcgt cctggtgaac cgcggctcgc gcgtcaccgc cgaccactcc gtcgaggtcg
      541 acaacgagcg cgcggccgag acggtcgtgg cgcacctcgc atcgctcggc caccggcgga
      601 tcgcccacat cgccggaccc ctcagcacca ccaccggcgc cgagcgcgcc gcgggctatc
      661 gcgcggcgat gaccgcgcac ggcctcggcg cgcacgtgct cgtcgtcgag gcgagcgcgt
      721 attcggagga ggccggccgc gacgcggccg gcaaggtgct cggcgcggac gtgacggcga
      781 tcttcgcggc gaacgacctc atcgccatcg gcgtgctgct cgcgctgcgc gagatgggcc
      841 gccgggtgcc ggcggacgtg tcgctggtcg gcgtgaacga catcccgctc gcgggcctcc
      901 tcgatccgcc gctcacgacg atgcgcgtgc cgcagcgtga gatgggcggc atcgcggcac
      961 ggatgctcat cgcactgctc gaaggtgagc cggtcacgcg gcgccacgtg gtgctcgaga
     1021 cctcgctcgt cgttcgcggc tccaccgcgg cgccggcggg ccgggggagg atgtcatgac
     1081 ggcgatggcg gggatccgca tctcggtcga gtccgcgacg aagcacttcg tcaccggcga
     1141 cgtgaccgtc ttcgacggtc tcgatctcca ggtgcgcgag ggcgaggcgc tctgcatcct
     1201 cggcccgtcc ggctgcggca agacgacgct cctgcgctgc atgcacggac tcgcgacgct
     1261 gaacagcggt cgcatcgtca tcgacggcag cacggtgtcc acgccgcggc gcaacgtcgc
     1321 gatggtcttc cagcacttcg gcctctttcc gtggaagcgg gtgcgcgaga acgtcgcgta
     1381 cggcctcgag ctggcgggcg cgagcagggc caccatcgcg gagcgcgtgg agcgctacat
     1441 cgacatggtc ggtctgcggg gcttcgagga cgcctacccc tacgcgctct caggcggcat
     1501 gcagcagcgc gtgggcctcg cgcgggcgct ggcggtggag cccgaggtgc tgctcatgga
     1561 cgagccgttc ggatcgctcg acgcgctcac gcgcgaggtc ctccaggacg agctgctgcg
     1621 catctatcag gaacggcccc gcaccatggt gttcatcacg cactccatcg aggaggccat
     1681 cgcgctgggc gaccgcgtcg tggtgctctc ggctcgacca tctcgcgtgc gcgagatcat
     1741 ctcggtcgac ataccgcgac cgcggaccgt cgagtcagtg atcgcgcacc cgcacttcgt
     1801 cgagctccgc gatcgctgct ggcgactgct cagggaaaag gcgaccgcat gaccatccga
     1861 gaagcgcagg cgcgcgcgag ctccgcggtc cggaggcgcg gcttccggcc ccgaggcggc
     1921 gattggggcg tgcgcctggc gtccatcgcc gtgttcctgc tcatctggga attcctcgcg
     1981 cccggctgga acccgctgat catccg
//
LOCUS       DASTZR010000135         2797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_269150, whole genome shotgun sequence.
ACCESSION   DASTZR010000135 DASTZR010000000
VERSION     DASTZR010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269150"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="VFV20_03085"
     CDS             <1..1138
                     /locus_tag="VFV20_03085"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU5287362.1"
                     /translation="AQDRTEREIVDLIVRDGPAAQAIRAEAEVTRREQLMRLAYPNPG
                     VLYSREGAGFTEFLQVEQSFPSFGVRAALSRAGVRATAAAEAERDARLWMLRADAAAV
                     VARLIAAEARVAAGQAHAREAERLFEILRTREREGEGSRFDRVRAEQELRDVRQALAA
                     AVVDVAEARAALGAMLPRGVHVTAVASAPDLRSPPPIDALATRAAETRAELRALREAA
                     ARADLESDAARLARRPSPTFFGGLKRADTSRRETGGLFGVSMAVPLFDGGGREAARWT
                     AERARVDAEREAMGQRIQSEISGASETLAVRQAALAQDADGGGDELVTIAEVAYREGE
                     IGILELIDAFRTASRARLRTIELRLGARLAQIALERAVGDVLWP"
     gene            1129..2658
                     /locus_tag="VFV20_03090"
     CDS             1129..2658
                     /locus_tag="VFV20_03090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5287363.1"
                     /translation="MALTHLRWICLALVVLPACGRASQPAAETTKAEALSVTRWTAKT
                     ELFAEYAPLVVGETSRFAIHLTRLDTFKPLPDGHVEVRLEGGASTEVFRVDAPSRPGI
                     FGVDVKPAHAGARQLVIVVRASGVSDEHRVGDVTVHGNAAAAQAARETGGDAGGVSFL
                     KEQQWALDFATAVVQEQPVRESLRVPARIEARPGGAADVAAPIDGRLARVLDLPLGAT
                     VSRGQELARLQPPPSSPADLPQLQRARADAQTSLSLATRDRERAERLVAAGAAPQKRL
                     EDARAAEQQARAGLTAADASLAQFHAARGAGTPGAAGLFVVRAPVTGVIASRDATTGA
                     NVTAGTTLFRVVDASQVHVVGRVTEADASRVRLVTGGEIEVTGQATRTPAGRLVGVGR
                     VLDPESRTLAITFAFDNRVAKLPVGQAVFLHLLMDAAAARPTVPAGAVVDDAGRPIVF
                     VQREGETFERRPVTLGPRAAGIVQILEGLSPGERVVTKGASLVRLASLSTSVPAHGHV
                     H"
BASE COUNT          398 a          973 c         1018 g          408 t
ORIGIN      
        1 cgcgcaggac aggaccgagc gtgaaatcgt cgatctgatc gtgcgggacg gtccggcggc
       61 gcaggccatc cgcgcggagg cggaggtgac gcggcgggag cagctcatgc gtcttgccta
      121 tccgaatccc ggagtgctct acagccgtga aggagccggc ttcacggagt tcctccaggt
      181 ggagcaatcg ttcccgagtt ttggcgttcg cgcggcgttg tcgcgcgcag gagtccgcgc
      241 gacggccgcg gcggaggcgg agcgcgacgc gcggctctgg atgcttcgcg ccgatgccgc
      301 tgcggtggtg gcgaggctca tcgcggccga agcccgtgtc gccgccgggc aggcacacgc
      361 ccgtgaagcc gaacggctgt tcgagatcct ccgcactcgg gagcgggagg gtgaaggatc
      421 gcgattcgat cgcgtccgcg cagagcagga attgcgcgat gtgcgccagg cgctcgcggc
      481 agccgtggtg gatgtagccg aagcgcgcgc cgcgctcggc gccatgctcc cacgcggcgt
      541 tcacgtcacg gcggtcgcgt ccgctcccga tctgcggtcc cctcccccga tagacgcgct
      601 cgccacgcgt gccgctgaga cacgcgctga actccgtgcg ctccgcgagg cggccgcgcg
      661 cgcggatctc gagtcggacg ccgcgcgact cgcccggcgg ccgtcgccga cgttctttgg
      721 tggactcaag cgcgccgaca cgtcacggcg ggagaccggc ggcctcttcg gcgtctcgat
      781 ggccgtgccg ttgttcgacg gcgggggccg cgaggctgcc cgctggactg cggagcgggc
      841 gcgcgtcgat gccgagcggg aggcgatggg acagcggatt cagagcgaga tcagcggcgc
      901 ctccgagacg ctcgccgtca ggcaggcggc gctcgcgcag gacgccgacg gcggcggcga
      961 cgagctcgtc acgatcgccg aggtcgcgta ccgggaaggg gagattggga tcctcgagct
     1021 catcgacgcg tttcggaccg cttcgcgagc gaggcttcgc accattgagt tacggctcgg
     1081 cgcacgcctg gcacaaatcg cgctggaacg cgcggtggga gacgtcctgt ggccctgaca
     1141 cacctccgct ggatctgcct cgccctcgtc gtcctgcccg cgtgcggtcg cgcatcgcag
     1201 ccagcagccg aaacaacgaa agccgaggca ctcagcgtga cgcggtggac ggcaaagacg
     1261 gagctgttcg ccgagtacgc gcctctcgtt gtgggcgaga cctcgcgctt tgccatccac
     1321 ctgacgaggc tcgacacgtt caaaccgctg ccggacggtc acgtcgaagt tcgtctcgag
     1381 ggaggcgcct cgacggaggt ctttcgcgtg gacgcgccgt cgcgccccgg catcttcggc
     1441 gtcgacgtca agccggcaca cgccggcgcc cggcaactcg tcatcgtcgt ccgagcttct
     1501 ggagtcagcg acgagcatcg ggtgggagat gtcaccgtcc atggaaatgc cgcagccgcg
     1561 caggcggcac gcgagacggg cggggatgcc ggcggtgtca gcttcctgaa agagcagcag
     1621 tgggccctcg acttcgcgac cgctgtcgtc caggaacagc cggtgcggga gtccctgcgc
     1681 gtcccggcca gaatcgaagc gcgacccgga ggcgccgcgg atgttgccgc ccccattgac
     1741 ggacggctgg cgcgggtcct cgacctaccg ctcggtgcga cggtgtcccg cgggcaggaa
     1801 ctcgcgcgcc tgcagccgcc cccttcgtcg ccggcggatc ttccgcaact ccagcgtgcg
     1861 cgagccgacg cgcagacgtc gctctcgctc gcgacgcgcg atcgcgagcg agccgagcgc
     1921 ctcgtcgccg cgggcgcggc gccccagaag cgactcgagg acgcgcgcgc ggcggagcag
     1981 caggcccggg cgggcctcac ggcggcggat gccagcctgg cgcaattcca cgcggcgaga
     2041 ggagcaggca cgccgggtgc tgcgggtctg ttcgtcgtcc gtgcgccggt gaccggcgtc
     2101 atcgcctcgc gagatgcgac aaccggcgcg aacgtcaccg ccggaacgac actcttccgc
     2161 gtcgtcgatg cctcccaggt ccacgtcgtc ggacgggtga ccgaagcaga tgcgtcgagg
     2221 gttcgtctcg tcaccggcgg agagatagaa gtgaccgggc aagccacccg aacgccagcc
     2281 gggcgcctgg tcggcgtcgg ccgcgtgctc gacccggagt cccggacgct cgcgatcacg
     2341 ttcgccttcg acaaccgcgt ggcgaaattg ccggtgggcc aggccgtgtt ccttcacctg
     2401 ttgatggatg cggcagcggc gcgccccacc gtgcccgccg gagcagtcgt ggacgatgcc
     2461 ggacggccca tcgtgttcgt gcagcgcgag ggcgagacat tcgagcggag gcccgtcacg
     2521 ctgggcccac gcgccgccgg aatcgtccag atcctcgaag gcttgagccc gggcgagcgg
     2581 gtcgtcacga agggcgcgtc cctcgtccgg ctcgcgtcgc tgtccacgtc ggtccccgct
     2641 cacggccacg tccattgacc ggggccaaat catgatcgac gcactcatcc ggtggtcgct
     2701 cgcgcatcgc gctgccgtcg tggcgctcgc cgccgcgttc ctggtgtggg gcggctggac
     2761 ggcctcgcgc attccgctcg acgtgctgcc ggacctc
//
LOCUS       DASTZR010000136         2299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1052777, whole genome shotgun sequence.
ACCESSION   DASTZR010000136 DASTZR010000000
VERSION     DASTZR010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052777"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..771
                     /locus_tag="VFV20_03095"
     CDS             178..771
                     /locus_tag="VFV20_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287364.1"
                     /translation="MKNATGVELLPQRPQYVRERAEPAGHILEPEWRGTRVLIRVGQE
                     GPRFTGYAGQVEGPRELYDAIVADTQAASAIFDGVLVDDWRDEAELEMDHEGNAMTRV
                     TSGRRIFAAFDLLEVDGTSLLDVPLLERRRHLEGVLAASVNVRLTPFVTRGLRSWRDT
                     LTGQGFQRVVLKNWNSTYAPGRTTSDWLVVEKINPAR"
     gene            786..2063
                     /locus_tag="VFV20_03100"
     CDS             786..2063
                     /locus_tag="VFV20_03100"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEU5287365.1"
                     /translation="MATAKSAVIDHTQRDPWSVAQEQFGIAADRLHLDPNMRGILAEC
                     QRELTVHFPVRMADGAVKVFTGYRVQHNVSRGPGKGGIRYHPSVTLNEVKALAMWMTW
                     KCAVVGIPYGGAKGGVVCDPKTMSAQQLETMTRRYATEIMLLIGPERDIPAPDVNTNA
                     QTMAWIMDTYSMHHGYTAPAVVTGKPISIGGSEGRNEATARGAVYTIIEAAKHLGMDL
                     EDARVVVQGFGNAGSFSAKLIAELGAKVLAVSDTSGGVYNAKGIDPNKVDAYKKETGS
                     VVGFPGADKVTNTELLELDCDILIPAAIENQIGEHNAPKIKAKIVAEAANGPTSPEAD
                     RILFDRGVFLIPDILCNAGGVTVSYFEWVQDLQNLLWREATINARLKEVMVKSFNDVL
                     DMSKKHKVDMRTAAYMVAVHRVAEATLTRGIYP"
     gene            2203..>2299
                     /locus_tag="VFV20_03105"
     CDS             2203..>2299
                     /locus_tag="VFV20_03105"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287366.1"
                     /translation="METTGFDPELDRIVEIGAVRVRLERDGTLTAA"
BASE COUNT          421 a          730 c          802 g          346 t
ORIGIN      
        1 cgcgcggcgg cacccgggtc gcggatgccg ccggcgtgcg gcgtggtgtg ggatgggtca
       61 tttcgacgct ggcaatggcc atttcagggc tcccctctgg ttcgttcgaa cagacgttcg
      121 catattagaa cgcctgttct ggctgtcaag aggatgggat actcgggccg aaagccattg
      181 aaaaacgcca ccggggtcga gctcctgccc caacgtcccc agtacgtccg cgagcgggca
      241 gagccggcgg ggcacatcct cgagccagag tggagaggca cccgggtgct catccgcgtg
      301 gggcaggagg ggccacgctt tacgggatac gccggacagg tggagggacc gcgtgagctc
      361 tacgacgcca tcgtggccga cacgcaggcc gcgagcgcga tcttcgacgg cgtgctcgtg
      421 gacgactggc gcgatgaggc ggagctggag atggaccacg aaggcaacgc catgacgcgc
      481 gtcacgagcg ggcgccggat cttcgcggcc ttcgacctgc tcgaggtcga cggcacgtcg
      541 ctgctggatg tcccgctgct ggagcggcgc cggcacctcg agggcgtgct cgcggcgagc
      601 gtgaacgtcc ggctgacgcc gttcgtcacg cgcgggctcc gctcctggcg cgacacgctg
      661 acgggtcagg gtttccagcg tgtcgtgctg aagaactgga acagcaccta cgcaccaggt
      721 cgcacgacga gcgactggct cgtcgtggag aagatcaacc ccgcgagata gggacggagg
      781 gatacgtggc cacagccaag tcggcggtca tcgatcacac ccagcgcgat ccgtggtccg
      841 tggcgcagga gcaattcggg atcgcggcgg accgtctgca cctcgacccg aacatgcgcg
      901 gcatcctcgc ggagtgccag cgcgagctga cggtccattt cccggtgcgc atggccgacg
      961 gtgcggtgaa ggtcttcacc ggttaccggg tccagcacaa cgtgtctcgc ggccccggga
     1021 agggcggcat ccgctaccac ccgtcggtca cgctcaacga ggtcaaggcc cttgcgatgt
     1081 ggatgacgtg gaagtgcgcc gtggtgggca tcccgtacgg tggcgccaag ggcggcgtcg
     1141 tctgcgaccc gaagacgatg agcgcgcagc agctcgagac catgacgcgc cggtacgcga
     1201 cggagatcat gctgctcatc ggccccgagc gggacatccc cgcaccggac gtcaacacga
     1261 acgcgcagac gatggcctgg atcatggaca cgtactcgat gcatcacggg tatacggcgc
     1321 cggcggtcgt gaccgggaag ccgatctcga tcggcggcag cgagggccgc aacgaggcga
     1381 ccgcgcgcgg cgcggtctac acgatcatcg aggcggcgaa acatctcggg atggacctgg
     1441 aggacgcgcg cgtcgtcgtg cagggcttcg gcaacgccgg ctcgttctcc gcgaagctca
     1501 tcgcggagct cggcgcgaag gtgctcgccg tgtcggacac gagcggcggc gtctacaacg
     1561 cgaagggcat cgatcccaac aaggtcgatg cgtacaagaa ggagaccggc tcggtcgtcg
     1621 ggttccccgg cgcggacaag gtcaccaaca cggagctcct cgagctcgac tgcgacatcc
     1681 tgatccccgc ggcgatcgag aaccagatcg gcgagcacaa cgcgccgaag atcaaggcga
     1741 agatcgtcgc cgaggccgcg aacgggccga cgagcccgga ggccgaccgc atcctcttcg
     1801 acaggggcgt gttcctcatc ccggacatcc tgtgcaacgc cgggggcgtc accgtctcgt
     1861 acttcgagtg ggtgcaggac ctgcagaacc tcctctggcg cgaggcgacg atcaacgccc
     1921 gcctcaaaga ggtcatggtc aagtccttca acgacgtgct ggacatgtcg aagaagcaca
     1981 aggtggacat gcgcaccgcg gcgtacatgg tggcggtgca tcgcgtcgcg gaggcgacgc
     2041 tgacacgagg tatctaccct taatttgtga tgacgggagg tccctaatgg gacctccctc
     2101 catcagaatg ctggcagcat ccgtggcacc cgggaggtcc ctagggggac ctccctggca
     2161 atgccctaga aggcgtccct catcgatctc gtagccctgg acctggagac gaccggattc
     2221 gatccggagc tagaccgcat cgtcgagatc ggtgcggtcc gcgtgcgtct cgagcgcgac
     2281 gggacgctga ccgctgcgg
//
LOCUS       DASTZR010000137         2714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1156992, whole genome shotgun sequence.
ACCESSION   DASTZR010000137 DASTZR010000000
VERSION     DASTZR010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156992"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VFV20_03110"
     CDS             <1..335
                     /locus_tag="VFV20_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287367.1"
                     /translation="DVEVGDVITFLFYGQTTTHRVIAIDDGSEGRAFTTKGDANAAAD
                     PEPKLFHAQVGIVRGTIPFLGYVLVYLRSYAWIALAAAATAVFVMSAVQVAGSLRSQR
                     AQPRGSRA"
     gene            332..1369
                     /locus_tag="VFV20_03115"
     CDS             332..1369
                     /locus_tag="VFV20_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEU5287368.1"
                     /translation="MTRAIVSLLAGTLAVATIATFGTSSAAFTARTANPSNQAATLLV
                     QPPTGTSATSATAGAITLSWPASPTAPGTGHTLSYRVFRGPVGGPYAQIGSTSASVLT
                     YTDTPASDGTYSYVVETAVSGIGTFTSVNSTAASAISDRTPPAMSITCGGSACGTGWY
                     TAAVSVTVSGTDSGVGMGSLSYQIDGGTTTTVSAASASFSVSGDSAGHTVAYYGRDAA
                     NNTSTTATQTIRIDATAPTAATSVQSTQGTKGAPATINLTWMAGTDALSGVQGYVIYW
                     SNAVSGGGCPAISAANYPNSAAVGPVTAATIPPSGTLKGGAQYCAYLVTIDNGGNMSA
                     PSNVTGPTKAN"
     gene            complement(1407..>2714)
                     /locus_tag="VFV20_03120"
     CDS             complement(1407..>2714)
                     /locus_tag="VFV20_03120"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU5287369.1"
                     /translation="GCDKTIPGTVMALARLDVPSLMLYGGSIAPGRFRGHDVTIQDVF
                     EAVGAHAAGNMSDTDLSELEHAACPGAGACGGQFTANTMATAFEALGISPAGSSGVPA
                     TDRDRDEVMRAAGRRAVEMLRADFRPSRVLRRENFENAIAAVMATGGSTNAVLHLLAC
                     ARESGAELSIDDFDRISAKTPLLADMKPWGRFTAPDMHRAGGIMLVLQRLQQAGLLNE
                     KAMTVTGRTIGDEARAAMEAPKQEVVRPLADPLAKSGGMVILKGSLAPEGCVMKVAGH
                     AMSARRVSARVFDRAEDAFAAVVGKKIADGDCLVIRYEGPKGGPGMREMLGVTGAIVG
                     AGLADKVALITDGRFSGATHGHVVGHIAPEAFVGGPIALVRDGDTILIDPERRRLDLE
                     VDPKELDKRRAAWKAPAPRYTRGVMAKYAKLVSSASEGAVTAS"
BASE COUNT          398 a         1004 c          932 g          380 t
ORIGIN      
        1 gggatgtcga ggtcggcgac gtcatcacct tcctgttcta cggacagacg accacgcacc
       61 gcgtcatcgc catcgacgat ggatccgagg ggcgcgcatt cacgacgaag ggggacgcga
      121 acgcggcagc cgaccccgag ccgaagctgt tccacgctca ggtcggcatc gtgcgcggca
      181 cgatcccgtt cctcggctac gtgctggtct acctgcggtc ctacgcgtgg atcgcgctcg
      241 cggccgccgc gaccgcggtc ttcgtcatga gtgccgtgca ggtggctggc tcgctgcgct
      301 cccagcgcgc gcagccgcgg ggatcgcggg cgtgacgcgc gcgatcgttt cgctgctcgc
      361 cggaacgttg gcggtcgcga cgatcgcgac cttcgggacg agcagcgcgg ccttcaccgc
      421 gcggacagcg aacccgtcga accaggccgc cacgctgctc gtgcagccgc cgaccggcac
      481 gagcgcgacc agcgccacgg ccggcgcgat cacgctttcc tggccagcgt cgccgaccgc
      541 cccgggcacc ggtcacacgc tcagctatcg cgtgttccgc ggccccgtcg gcggtccgta
      601 cgcgcagatc ggctcgacga gcgcgagcgt gctcacgtac accgacaccc cggcgagcga
      661 cgggacgtac agctatgtcg tcgagaccgc ggtcagcggc ataggcacgt tcacgagcgt
      721 caacagcacg gcggcctcgg ccatctcaga tcgcacgccg ccggcgatgt cgatcacgtg
      781 cggcggctcc gcgtgcggca ccggttggta cacggcggcg gtctccgtca cggtgagcgg
      841 gaccgactcg ggcgtcggca tgggatcgct ctcgtaccag atcgatggcg gcacgaccac
      901 caccgtctcc gcggcgagcg cgagcttcag cgtgagcggg gacagcgccg gccacaccgt
      961 cgcgtactac ggccgtgacg ccgcgaacaa cacgagcacc accgcaacgc agaccatcag
     1021 gatcgacgcg acggcaccca ccgcggccac gagcgtgcag tcgacgcagg gcaccaaggg
     1081 cgcgccggcc acgatcaacc tcacgtggat ggcgggcacg gatgcgctct cgggcgtgca
     1141 gggctacgtc atctactggt cgaacgccgt ctccggcggg gggtgcccgg cgatctccgc
     1201 ggcgaactat ccgaactcgg cggcggtcgg tccggtcacg gcggcgacga tcccgccgtc
     1261 cggcacgctc aagggcggcg ctcagtactg cgcctatctc gtcacgatcg acaacggcgg
     1321 caacatgagc gcgccgagca acgtgacggg accgacgaaa gcgaactagc gcgtgaagcg
     1381 tcgcgggatc acctaggagc gcagcctcag ctcgcggtaa cggctccctc gctggcactc
     1441 gatacgagct tcgcgtactt cgccatcacg ccgcgggtgt agcgcggcgc cggcgccttc
     1501 cacgcggcgc ggcgcttgtc gagctccttt gggtccacct cgaggtcgag gcggcgcctc
     1561 tccggatcga tgaggatcgt gtcgccgtcg cgcaccagcg cgatcggacc accgacgaac
     1621 gcctcgggcg cgatgtgacc gacgacatgc ccgtgcgtcg cgccggagaa gcggccgtcg
     1681 gtgatgagcg cgaccttgtc cgcgagcccc gcgcccacga tcgcgccggt gacgccgagc
     1741 atctcgcgca tccccgggcc gcccttcggt ccctcgtagc ggatgacgag gcagtcgccg
     1801 tccgcgatct tcttccccac caccgcagcg aacgcgtcct ccgcgcgatc gaagacgcgc
     1861 gcgctgacgc ggcgcgcgct catcgcgtgg cccgccacct tcatcacgca gccctcgggc
     1921 gcgagcgacc ccttcaggat caccatgccg ccgctcttcg cgagcgggtc cgcgagcggc
     1981 cgcaccacct cctgcttggg cgcctccatc gcggcgcgtg cctcgtcgcc gatcgtgcgc
     2041 ccggtgacgg tcatggcctt ctcgttcagc aggccggcct gctgcagccg ctggagcacg
     2101 agcatgatgc cgcccgcgcg atgcatgtcc ggcgcggtga agcggcccca cggcttcatg
     2161 tccgcgagca gcggcgtctt cgcgctgatg cggtcgaagt cgtcgatcga cagctcggca
     2221 ccggactcgc gcgcgcacgc gagcagatgg aggaccgcgt tcgtcgagcc gccggtcgcc
     2281 atgaccgcgg cgatcgcgtt ctcgaagttc tcgcgccgca ggacgcgcga cgggcggaag
     2341 tcggcgcgca gcatctcgac cgctcgccgt ccggcggcgc gcatgacctc gtcgcggtcc
     2401 ctatcggtcg cgggcacgcc gctcgagccc gcgggcgaga tgccgagggc ctcgaacgcc
     2461 gtcgccatcg tgttcgcggt gaactggccg ccgcacgcgc ccgcgcccgg acaggccgca
     2521 tgctcgagct cggacaggtc ggtgtcgctc atgttccccg cggcgtgcgc gcccacggct
     2581 tcgaacacgt cctggatcgt gacgtcgtgg ccgcggaagc gcccgggcgc gatcgagccg
     2641 ccgtagagca tcagcgacgg gacgtcgagg cgcgccagcg ccatcacggt gccggggatc
     2701 gtcttgtcgc atcc
//
LOCUS       DASTZR010000138         4585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1026584, whole genome shotgun sequence.
ACCESSION   DASTZR010000138 DASTZR010000000
VERSION     DASTZR010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026584"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VFV20_03125"
     CDS             complement(<1..256)
                     /locus_tag="VFV20_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5287370.1"
                     /translation="MSSVSVDLSAKAALVTGAASGIGRAIAEDLARRGARVVLADIDG
                     EGVRSVAAGLPGAIAVRADISSREDCRALVEQARSALGGVD"
     gene            complement(345..1250)
                     /locus_tag="VFV20_03130"
     CDS             complement(345..1250)
                     /locus_tag="VFV20_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287371.1"
                     /translation="MRKIFIPTLAFALFALAACNQATTPGSSGSPGASGSAVPRATGS
                     VAASASAGASAKEITLQVTVPEENSKAGVNGTGWTVDVIAKGNGPNMDKVKPAFRATT
                     SSGKNTAFPGLVVLIRPVGSSAQTSTASPSAASQPNLAGLFQIIGLPNMLGSTASISS
                     VNTATGSPAASATGGAGATGASGAASASARPSGTAATRSTTTTDNDTAEATWFVQQSL
                     WGTDVDVEMTVFVVDGDAPDAVTDRSSLKIISNEITVRFHINGNGTASPTGSAAPSGS
                     ASPAGSPSTSPSGTARPSASPTSTP"
     gene            complement(1325..1717)
                     /locus_tag="VFV20_03135"
     CDS             complement(1325..1717)
                     /locus_tag="VFV20_03135"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEU5287372.1"
                     /translation="MERVEHVDHVRERPVYPATSANTNVNATTGRVATPVWTVTSVVT
                     LIFTVLEVLLLLRFIFKLAGANANQPLISALYRLTDALTRPFQGIFPEPVGPPVVDVA
                     ALLAIVFLFLVGALIVALVRAITAPRGG"
     gene            1801..2241
                     /locus_tag="VFV20_03140"
     CDS             1801..2241
                     /locus_tag="VFV20_03140"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEU5287373.1"
                     /translation="MEAPFTFSYRQRVRFGETDTQQVVYYANYLLYAEVGRMEYLRHL
                     GLDYQHSFMDRGLDFTIGEAHVKYRAPLRFDDLFDIKMRVGEVRHSSWAFEYAIDRAD
                     GLHCADVTTVQVVISREAKRAARMPDELRLLLEGTKLSKGVTGA"
     gene            2250..3395
                     /locus_tag="VFV20_03145"
     CDS             2250..3395
                     /locus_tag="VFV20_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5287374.1"
                     /translation="MDTELPEELQLLQKSIRKFVEEKLAPHEKEIEDRNEISREILNA
                     MAEAGLFGIGHDERWGGQGFGKLGYCVAIEQAGRVNASLWNVVGGSAGLCGTAIEIGG
                     PDDVRERYLPQIFGGEKIGAYGLSEPGAGSDAGSLKTQAVRQGGEYVIDGFKTFITNA
                     PIADVFVVFANVAPDRGSKGITAFVVERGTEGLEVGHNDEKMGLHGSTTAQLYFSNMR
                     VPAANRVGEEGQGFRIALATLDVGRLGLAAHSVGIAQRLLEASAQYAKSRVQFGKPIS
                     ANQAIQWMLADAATEIHAARLMVSDAAARADKGERITQRAAMTKLFATETLGRVADAA
                     VQIHGGMGYMRELWIERAYRDARIARIYEGTSEVQRMVIAGGLLENT"
     gene            3484..4269
                     /locus_tag="VFV20_03150"
     CDS             3484..4269
                     /locus_tag="VFV20_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287375.1"
                     /translation="MENLGVLIAVTAGAALTGLAIWVARYQFGLRRATGAMLDAEDAL
                     DSGEIERARSLMAPVLSRFPKLAVVQEVAADVLYAGGDPLSAASLWESALKKLGAPRV
                     APRLVAAYAALNRPGDARRVAALVPDDALARITLAWCELAAIGGDRGRGRTLADEFAR
                     DTAVRATPSGDAMAAVLSAIAAAQHGDAAGMRAGLDRAQARSAELAPHDRAFIGYLGG
                     IALRETGALEDARSTWTIAMEAAPESIGAALARRERSHLPAAS"
     gene            complement(4266..>4585)
                     /locus_tag="VFV20_03155"
     CDS             complement(4266..>4585)
                     /locus_tag="VFV20_03155"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HEU5287376.1"
                     /translation="VLFDRTSSYHELTSSLFRQAGVVPASTLELDNVEAAKKMVQQGL
                     GVALLPRLALQSELKARSLRTVKITGAPPVRRPIVALRRKDAGEALGHVAAFLELLKE
                     VRV"
BASE COUNT          695 a         1563 c         1673 g          654 t
ORIGIN      
        1 tgtccactcc gccgagcgca cttcgtgcct gttcgacgag cgcgcggcaa tcctcgcgcg
       61 acgagatgtc ggcacgcacc gcgatcgcgc ccgggagccc cgcggccacc gagcggacgc
      121 cctcgccgtc gatgtccgcg agcaccacac gcgcgccgcg acgggcgaga tcctcggcga
      181 tcgcgcggcc gatgccgctc gccgcgcccg tgaccagcgc ggcctttgcc gacaggtcca
      241 cgctgaccga gctcatcgcg gcgacgatag ccgtcccggc cgcgcacggt gaggggcggt
      301 cacggggacc gcccctcacc taccgcatcg cgctgatcga ccgcttaggg cgtggatgtc
      361 ggggacgcgc tcggacgcgc ggtacccgac ggggacgtgg acggcgaacc cgccggactc
      421 gccgatccgc tcggtgctgc cgagccggtg ggcgaggcgg tgccgttgcc gttgatgtgg
      481 aagcggaccg tgatctcgtt ggagatgatc ttgagcgagc tccggtcggt caccgcatcc
      541 ggagcgtcgc cgtcgacgac gaagaccgtc atctcgacat cgacatcggt gccccagagg
      601 gactgctgga cgaaccaggt cgcctccgcg gtgtcgttgt ccgtcgtcgt cgtggaacgt
      661 gtcgccgctg tacccgatgg ccgcgcgctc gcgctggccg cgcccgaggc accggtcgcg
      721 ccggcaccgc cggtcgcgct cgccgcgggc gagccggtgg ccgtgttcac cgaggagatg
      781 ctcgcggtac tgccgagcat gttcgggagt ccgatgatct ggaagagacc cgccaggttg
      841 ggctgcgatg ccgcggacgg cgacgcggtc gacgtctgcg cgctcgagcc gacgggccgg
      901 atcagcacca cgaggcccgg gaacgccgtg ttcttcccgc tgctcgtcgt ggcgcggaac
      961 gcgggcttga ccttgtccat gttcgggccg ttgcccttgg cgatcacgtc gaccgtccag
     1021 ccggtgccgt tgaccccggc cttcgagttc tcctcgggga cggtgacctg aagggtgatc
     1081 tccttcgctg aggcacccgc ggacgcggac gcggccaccg acccggtggc tcgcgggacc
     1141 gctgaaccgg atgcgccggg agaccctgag gacccgggcg tcgtcgcctg gttgcaggcg
     1201 gcgagcgcga aaagtgcgaa cgcgagtgtg gggatgaaga tctttctcat gccccgccgt
     1261 aaagcacctc gcatgccgct cacggcgagg gcacgctcgc gcgtaccccc gccgtccgtg
     1321 cgttctagcc gccgcgcggt gccgtgatcg cgcggacgag agcgacgatg agcgcgccga
     1381 cgaggaacag gaacacgatc gcgaggagcg cggccacgtc cacgaccggc ggccccacgg
     1441 gctccgggaa gatgccctgg aacggacgcg tgagcgcatc ggtgaggcga tacagcgccg
     1501 agatcagcgg ctgattcgcg ttcgcgcctg ccagcttgaa gatgaagcgg agcagcaaca
     1561 ggacctcgag caccgtgaag atgagcgtca cgacgctcgt gacggtccag accggcgtcg
     1621 ccaccctccc tgtggtggcg ttcacgttcg tgttcgcgct ggtcgcggga tagacggggc
     1681 gttcgcgcac gtggtccacg tgctcgactc gctccacgcg ctctgttggg tgttcgtcgt
     1741 gtcgaattgc catgtcttcg gagcgagcag ggtccgtgcc aagccggtaa cctgccgagc
     1801 atggaggcgc cgttcacctt ctcgtaccgc cagcgggtgc gctttgggga gaccgacacg
     1861 caacaggtcg tctactacgc gaattacctc ctctacgccg aggtgggccg catggagtac
     1921 ctccggcatc tcggcctgga ctaccagcac agcttcatgg accgtggcct ggacttcacg
     1981 atcggcgagg cacacgtgaa gtaccgcgcc ccgctccgtt tcgacgacct gttcgacatc
     2041 aagatgcgcg tcggcgaggt ccgccactcg tcgtgggcat tcgaatacgc gatcgaccgc
     2101 gccgacggac tgcactgcgc cgacgtcacg acggtccagg tcgtcatcag ccgggaggcc
     2161 aagcgagccg ctcgcatgcc cgacgagctg cggctgctgc tcgaaggaac gaagctgtcg
     2221 aagggggtga cgggggcgta gcccctccca tggatacaga gctccccgag gagctgcagc
     2281 tcctccagaa gtccatccgc aagttcgtcg aggagaagct cgcgccacac gagaaggaga
     2341 tcgaggaccg caacgagatc tcgcgcgaga tcctgaacgc gatggccgag gccggcctgt
     2401 tcgggatcgg ccacgacgag cgttggggcg gccaggggtt cggcaagctc gggtactgcg
     2461 tggccatcga gcaggccgga cgcgtgaacg cgtcgctgtg gaacgtcgtc ggaggttccg
     2521 cgggcctctg cggcacggcg atcgagatcg gcgggcccga cgacgtgcgc gagcgctacc
     2581 tgccgcagat cttcggcggc gagaagatcg gcgcgtacgg gctctccgag cccggcgccg
     2641 gcagcgacgc cggatcgctg aagacgcagg cggtgcgtca gggcggcgag tacgtcatcg
     2701 acggattcaa gacgttcatc acgaacgcgc cgatcgccga cgtgttcgtc gtcttcgcga
     2761 acgtcgcgcc ggaccgcggc agcaagggca tcaccgcgtt cgtcgtcgag cgcggcaccg
     2821 aggggctcga ggtcgggcac aacgacgaga agatggggct ccacgggtcg accacggcgc
     2881 agctgtactt cagcaacatg cgcgtccccg cggcgaaccg cgtgggcgag gaggggcagg
     2941 gcttccgcat cgcgctcgcg accctcgacg tcggtcgcct cgggctcgcc gcgcattccg
     3001 tcggcatcgc acagcgactg ctcgaggcga gcgcccagta cgcgaagtcg cgcgtgcagt
     3061 tcggcaagcc catctcggcg aaccaggcga tccagtggat gctggcggac gcggcgacgg
     3121 agatccacgc cgcgcgcctc atggtctccg acgccgccgc gcgcgcggac aagggggagc
     3181 gcatcacgca acgcgcggcg atgaccaagc tgttcgccac ggagacgctc ggccgcgtcg
     3241 ccgatgccgc ggtgcagatc cacggcggga tgggctacat gcgcgagctc tggatcgagc
     3301 gtgcctaccg cgacgcgcgg atcgcgcgca tctacgaggg cacgagcgag gtccagcgga
     3361 tggtcatcgc cggtgggctg ttggagaaca cctaagccgc cgagggaccg agcggtgttg
     3421 tggataagcg tcgccgtggc gtggataacg cccgcgggcg tgcgggagta ggtcggagac
     3481 gacgtggaaa acctgggcgt actcattgcg gtcaccgccg gcgccgcact gaccggtctc
     3541 gcgatttggg tcgcccgcta ccagttcggg ttgcgccgcg cgaccggcgc gatgctggac
     3601 gccgaggacg ccctcgacag cggggagatc gagcgcgcgc gctccctcat ggcgcccgtc
     3661 ctctcgcggt tcccgaagct cgcggtggtg caggaggtcg cggccgacgt gctgtacgcg
     3721 gggggcgacc cgctctcggc ggcgtcgctt tgggagtccg cgctgaagaa gctcggcgcg
     3781 ccacgcgtcg cgccgcgcct ggtggccgcc tatgccgcgc tgaaccgccc gggtgacgcg
     3841 cggcgcgttg ccgcgctcgt gcccgacgac gcgctcgcac gcatcacgct cgcgtggtgc
     3901 gagctcgccg ccatcggcgg ggaccgcggg cgcgggcgga cgctcgcgga cgagttcgcg
     3961 cgcgacacgg cggtacgcgc gacgccgtcg ggcgacgcga tggccgcggt gctctcggcg
     4021 atcgctgccg cacagcatgg cgacgccgcg gggatgcgcg ccgggctcga tcgcgcgcag
     4081 gcacgcagcg ccgagctcgc gccgcacgat cgcgcgttca tcggctacct cggcggcatc
     4141 gcgctccgcg agaccggcgc gctcgaggat gcgcgcagca cgtggacgat cgcgatggag
     4201 gccgcgcccg agagcatcgg tgccgcgctc gcgcgccgcg agcggagcca tctgccggcc
     4261 gccagctaga cccgcacctc tttcagcagc tcgaggaacg cggcgacgtg gccgagcgcc
     4321 tcgcccgcgt ccttgcggcg cagcgcgacg atcggacggc gcaccggcgg cgcgccggtg
     4381 atcttcacgg tgcgcagcga acgcgccttg agctcggact gcagcgccag gcgcggcagg
     4441 agcgcgacgc cgagcccctg ctggaccatc ttcttcgcgg cctcgacgtt gtcgagctcg
     4501 agcgtggagg cggggacgac gccggcctga cggaagaggc tcgaggtgag ctcgtgatag
     4561 ctggacgtgc ggtcgaagag cacga
//
LOCUS       DASTZR010000139         4942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_426062, whole genome shotgun sequence.
ACCESSION   DASTZR010000139 DASTZR010000000
VERSION     DASTZR010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426062"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1318
                     /locus_tag="VFV20_03160"
     CDS             <1..1318
                     /locus_tag="VFV20_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5287377.1"
                     /translation="TDVKFHDGSAMTSEDIVATYNKIIFPPQGVLSPRAGAYAVVEKI
                     EAPDKNTVVFKLKYTSASFLTNLASPWNFIYSAAKLKADIHWYEKNVMGTGPFTYVSN
                     TKGQDWVGKKFPDYFGKDKDGTKLPYLDGYKALIITDATAGVNAIRSKQAMIEFRGFT
                     PQQRDDLSRALPNDLAIQEAPWICVNYVVPNTKVKPFDDPRVRQALSLAIDRWAGSDA
                     LSKTTIVKDVGGLMRPKGPFAQKDEDLAKLKGFSKDSKAAKEQAKKLLADAGVPNLSF
                     KFLNRNITTPYEPVAVFLIDQWKQVGITATHDVKETSPYQADLRAGNFQVALDFNCDF
                     LDEPDLQLAKFWSASKAGKGLNFAFYDDDTLDKMIDAQSRETDPAKRLKLVSDIEKYA
                     MDEKAYQYPVLWWYRIIPHLNTVKGWRIGSNHYTNQDLTTVWLAKK"
     gene            1362..2309
                     /locus_tag="VFV20_03165"
     CDS             1362..2309
                     /locus_tag="VFV20_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5287378.1"
                     /translation="MRRYVVGRLLLMLPTLVGVAVITFVVMRLVPGDIVALRYAGSSV
                     PQEIIDKERTILGLDKPLWAQFLDWMAQLARFDLGQSLWTGNSVWSEVQVRLPLSLEL
                     AVIASIMAVVIAVPLGVVAAVKQDTWMDYAIRVFSVGGLAMPSFWIGIMLVLGTITFL
                     GWAPPLVYTSFFEDPVANLSQLILPAVSVGYRYSAVSMRMTRSTVLEVMREDYVRTAR
                     AKGLRESLVVVRHAVRNALLPVVTVISLEFAFLIGGLVVTEQVFNLNGIGKLLVDAVT
                     HRDYPLIQALVMLLSSVFVVVNFAVDMVYVALDPRIRYS"
     gene            2306..3238
                     /locus_tag="VFV20_03170"
     CDS             2306..3238
                     /locus_tag="VFV20_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020021157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5287379.1"
                     /translation="MTERGAVLEHDAEELARRRYVESTTRKRFFRFWREFIQRRPLGA
                     VGLFLMLLMIFAAVLADRIAPYDPTAISFKDLLAPPSADHLFGTDNFGRDVFSRVLFG
                     ARTALLVGFVASVVGCTAGLVLGIVGAYVGGRTDEIIQRLMDVLLSFPLIVIAIAVVA
                     ALGTGEDKIGNVIAAITVPIIPRVSRVVRSSALSVVQMPYIEATRSIGTRAMRIMFRH
                     VAPNVFAPYLIMMTAFLGQAILLEASLSFLGLGVFEPTPSWGLMLRGAGMQFLERAPW
                     LAIAPGIAISMAVFGFNLFGDSLRDALDPRLRTA"
     gene            3238..3948
                     /locus_tag="VFV20_03175"
     CDS             3238..3948
                     /locus_tag="VFV20_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5287380.1"
                     /translation="MLRARDVVRVHMGHYTVPAEFPRAPDLQGAAIVVTAHLVRHPDG
                     PILLDTGFALGHPKAEALFHPVVRPFDDALRELGLRTGDVRAVANCHLHVDHAGNNWR
                     FPRTPIFVRRVEHDAASSTLDYSLPDRTIDFPGARLELLGDEDAEIARGVRVIPTPGH
                     TPGHQSFVIEAREGRIVVAGQAFNDASSYARAVLAWRLDREEQPHPEYAPWVARLQEL
                     DPWRVTFAHDLAVWERGA"
     gene            complement(3945..4670)
                     /locus_tag="VFV20_03180"
     CDS             complement(3945..4670)
                     /locus_tag="VFV20_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006607691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEU5287381.1"
                     /translation="MEGPLILEACLNGDRLRAEHPDVPISADELAREAQAVVAAGADA
                     IHVHPREPGGRQTMGPRQCSAAIAAIRAAAPHTPVGVTTIASIEPDPARRVALLRRWT
                     EKPDFVSVNWSEEGAAALVIACVERDIGIEAGIWTEDDARRFVESDLAKFCLRALVEP
                     LSQSTAEALGTAERIVALLAPTGLPLVVHGHDRTTWAVLRWAVAHGHGIRIGLEDTLT
                     LEGGLPVRGNAELVAAAARLAGR"
     gene            complement(4655..>4942)
                     /locus_tag="VFV20_03185"
     CDS             complement(4655..>4942)
                     /locus_tag="VFV20_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU5287382.1"
                     /translation="TARIIPAEEALRAGLLSEVVADDDALHGRVAELVRTMSGHAPLT
                     VWATKEGLRRIRGRMLPDEGRDLVALCYTSEDFREGVRAFMEKRRPEWKGR"
BASE COUNT          747 a         1795 c         1603 g          797 t
ORIGIN      
        1 cacggacgtc aagttccacg acggcagcgc gatgacctcg gaggacatcg tcgcgaccta
       61 caacaagatc atcttcccgc cccagggcgt gctctcgccg cgcgcgggcg cgtatgccgt
      121 ggtcgagaag atcgaggcgc ccgacaagaa cacggtggtg ttcaagctca agtacacgtc
      181 ggcctcgttc ctcacgaacc tcgcgtcacc gtggaacttc atctacagcg ccgccaagct
      241 gaaggcggac atccactggt acgagaagaa cgtcatgggc acgggcccgt tcacgtacgt
      301 ctcgaacacc aagggccagg actgggtcgg caagaagttc ccggactact tcggcaagga
      361 caaggacggc acgaagctgc cgtacctcga tggctacaag gcgctcatca tcaccgacgc
      421 cacggcgggg gtgaacgcga tccgctcgaa gcaggcgatg atcgagttcc gtggcttcac
      481 gccacagcag cgtgatgacc tgtcgcgcgc gctgcccaac gatctcgcga tccaggaagc
      541 cccgtggatc tgcgtcaact acgtcgtccc gaacaccaag gtcaagccat tcgacgatcc
      601 gcgcgtccgt caggcgctct cgctcgcgat cgaccggtgg gccggaagcg acgcgctgtc
      661 gaagacgacc atcgtgaagg acgtcggcgg cctcatgcgg ccgaagggtc cgttcgccca
      721 gaaggacgag gacctcgcga agctgaaggg attcagcaag gacagcaagg ccgcgaagga
      781 gcaggccaag aagctgctcg ccgacgcggg cgtcccgaac ctgtccttca agttcctgaa
      841 ccgcaacatc accacgccgt acgagccggt cgcggtcttc ctcatcgacc agtggaagca
      901 ggtcggtatc accgccacgc acgacgtgaa ggagacctcg ccgtaccagg cggacctccg
      961 cgcgggcaac ttccaggtcg cgctcgactt caactgcgac ttcctcgacg agcccgacct
     1021 gcagctcgcg aagttctggt cggcgagcaa ggccggcaag ggcctcaact tcgccttcta
     1081 cgacgacgac acgctcgaca agatgatcga cgcgcagagc cgcgagaccg atccggccaa
     1141 gcggctgaag ctcgtcagcg acatcgagaa gtacgccatg gacgagaagg cgtaccagta
     1201 cccggtgctg tggtggtacc ggatcatccc gcacctcaac accgtgaagg gctggcgcat
     1261 cggctcgaat cactacacga accaggacct gacgacggtc tggctcgcga agaagtaagg
     1321 cgaattagcc aagcggcagg ggccgcccga cactaccgcc gatgaggcgg tacgtcgtcg
     1381 ggcggctcct gctcatgctc cccaccctcg tgggcgtggc ggtgatcacg ttcgtcgtca
     1441 tgcgcctggt cccgggcgac atcgtggcgc tgcgctacgc cggctcgagc gtcccgcagg
     1501 agatcatcga caaggagcgc acgatcctcg gcctcgacaa gccgctgtgg gcgcagttcc
     1561 tcgattggat ggcgcagctc gctcgcttcg acctcggcca atccctctgg accggcaact
     1621 cggtgtggtc cgaggtgcag gtccggcttc cgctctcgct cgagctcgcg gtgatcgcct
     1681 cgatcatggc ggtggtcatc gcggtcccgc tcggcgtggt ggccgccgtg aagcaggaca
     1741 cctggatgga ctacgcgatc cgtgtcttca gcgtcggcgg tctcgcgatg ccgagcttct
     1801 ggatcggcat catgctcgtc ctcggcacga tcaccttcct gggctgggcg ccgcccctcg
     1861 tctacacctc gttcttcgag gacccggtcg cgaatctctc gcagctcatc cttcccgcgg
     1921 tgtccgtcgg atatcgctac tccgcggtgt cgatgcgcat gacgcgatcc acggtgctcg
     1981 aggtcatgcg cgaggactac gtgcgcacgg cccgcgcgaa gggcctgcgc gagtcgctcg
     2041 tcgtggtgcg ccacgccgtg cggaacgccc ttctgcccgt cgtgacggtc atcagcctcg
     2101 agttcgcctt cctcatcggc ggcctggtcg tgacggagca ggtcttcaac ctgaacggga
     2161 tcggcaagct tctcgtcgac gcggtcaccc accgcgacta cccgctcatc caggcgctcg
     2221 tcatgctcct ctcgtccgtc ttcgtggtcg tgaacttcgc ggtcgacatg gtctacgtcg
     2281 ccctcgaccc gaggatccgg tactcgtgac cgagcgcgga gcggtgctcg agcacgacgc
     2341 ggaggagctc gcgcgccggc gctacgtcga gtcgaccacg cgtaagcgct tcttccgctt
     2401 ctggcgcgag ttcatccagc ggcgcccgct cggcgccgtg ggcctcttcc tgatgctgct
     2461 gatgatcttc gcggccgtgc tcgccgaccg catcgccccg tacgacccga cggccatctc
     2521 gttcaaggac ctgctcgcgc cgccctccgc ggaccatctc ttcggcaccg acaacttcgg
     2581 tcgcgacgtc ttctctcgcg tgttgttcgg cgcgcggacg gcgctcctgg tggggttcgt
     2641 cgcgtccgtc gtgggctgca cggcggggct ggtcctcggc atcgtgggcg cctacgtcgg
     2701 cggccgcacc gacgagatca tccagcgcct catggacgtg ctgctgtcct tcccgctcat
     2761 cgtcatcgcg atcgccgtcg tcgccgccct cggcaccggc gaggacaaga tcggcaacgt
     2821 catcgccgcg atcaccgtcc ccatcatccc gcgcgtgtcg cgcgtggtgc gttcgagcgc
     2881 gctgtccgtg gtgcagatgc cgtacatcga ggcgacgcgc tccatcggca cacgcgcgat
     2941 gcggatcatg ttccgccacg tcgcgccgaa cgtgttcgcg ccctacctca tcatgatgac
     3001 cgcgttcctc ggccaggcga tcctcctcga ggcctccctc tcgttcctcg gcctcggcgt
     3061 gttcgagccg accccgtcgt ggggactcat gctccgcggc gcgggtatgc aattcctcga
     3121 gcgcgcgccg tggctcgcga tcgcgccggg catcgcgatc agcatggccg tgttcggctt
     3181 caacctgttc ggcgactcgc tccgcgacgc gctcgatccg cgcctgcgca ctgcctgatg
     3241 ctccgcgcgc gcgacgtcgt ccgcgtccac atgggccact acaccgtgcc ggcggagttc
     3301 ccgcgcgctc ccgacctgca gggcgcggcc atcgtggtga ccgcgcacct cgtgcggcat
     3361 ccggacgggc ccatcctcct cgacacgggc ttcgcgctgg ggcacccgaa ggcggaggcg
     3421 ctcttccacc cggtggtgcg cccgttcgac gacgcgctgc gcgagctcgg cctccgcacc
     3481 ggcgacgtgc gggccgtggc gaactgccac ctgcacgtgg atcacgccgg caacaactgg
     3541 cgattcccac gcacgccgat cttcgttcgg cgcgtggagc acgacgccgc gtcgtccacg
     3601 ctggactact ccctgccgga ccggaccatc gacttccccg gcgcgcgcct ggagctcctc
     3661 ggcgacgagg acgcggagat cgcgcgcggc gtgcgcgtca tcccgacgcc cgggcacacg
     3721 cccggccacc aatcgttcgt catcgaagcg cgcgagggcc gcatcgtcgt cgcggggcag
     3781 gcgttcaacg acgcgtcgtc ctacgcgcgg gcggtgctcg cgtggcgcct cgaccgcgag
     3841 gagcagccgc atcccgagta cgcgccgtgg gtggcacggc tgcaggagct cgacccgtgg
     3901 cgcgtcacct tcgcgcacga cctcgctgtt tgggagcgcg gcgcctagcg tcccgcgagc
     3961 ctcgccgcgg cggcgacgag ctcggcgttg ccccgcaccg gcagcccacc ctcgagcgtc
     4021 agcgtgtcct caagcccgat acggatgccg tggccgtgcg cgaccgccca gcgcagcaca
     4081 gcccacgtcg ttcgatcgtg accgtgcacg acgagcggga gtccggtcgg cgcgaggagc
     4141 gccacgatgc gctccgccgt gccgagcgcc tccgcggtcg actgcgagag cggctcgacg
     4201 agcgcgcgca ggcagaactt cgcgagatcg ctctcgacga agcgccgcgc gtcgtcctcc
     4261 gtccagatgc ccgcctcgat cccgatatcg cgctcgacgc aggcgataac gagcgcggcg
     4321 gcgccttcct cgctccagtt caccgagacg aagtccggct tctccgtcca gcggcgcagc
     4381 agcgcgaccc ggcgcgccgg gtccggctcg atgctggcga tcgtggtcac gccgaccggt
     4441 gtgtgcggcg cggcggcgcg gatcgcggcg atcgcggccg aacactggcg cgggcccatc
     4501 gtctgccgtc cgccgggctc ccgcggatgc acgtggatcg cgtcggctcc ggccgcgacg
     4561 acggcctgcg cctcgcgcgc gagctcgtcc gcgctgatcg ggacgtccgg gtgctcggcg
     4621 cgcaggcggt cgccgttcag acacgcctcg aggatcagcg gcccttccac tccggtcgcc
     4681 gcttctccat gaacgcgcgc acgccctcac ggaagtcctc gctggtgtag cagagcgcga
     4741 cgagatcgcg accctcgtcg ggcagcatgc gcccgcggat ccggcgcagt ccctccttcg
     4801 tggcccaaac ggtcagcggc gcgtgaccgg acatcgtccg cacgagctcc gcgacacggc
     4861 catgcagcgc gtcgtcgtcg gcgacgacct ccgagagcag ccccgcgcgc agcgcctcct
     4921 cggcggggat gatccgcgcc gt
//
LOCUS       DASTZR010000140         2021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1207539, whole genome shotgun sequence.
ACCESSION   DASTZR010000140 DASTZR010000000
VERSION     DASTZR010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207539"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VFV20_03190"
     CDS             complement(<1..444)
                     /locus_tag="VFV20_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287383.1"
                     /translation="MVKEAATERAETSRDEQEHGARLGGVLALAERSERFAGLVERLE
                     TRAALTLADTSAGARAFAWSALVAVARRPIVLVAPSEDRARRWRAELAGWLGEERVLL
                     FPERETMPFEVGAPSAGAIHQRLLTLWRMHTAPGSEPIAVVAPLRA"
     gene            complement(437..1051)
                     /gene="pth"
                     /locus_tag="VFV20_03195"
     CDS             complement(437..1051)
                     /gene="pth"
                     /locus_tag="VFV20_03195"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU5287384.1"
                     /translation="MVRLAKLFDRSAPARVDLLVVGLGNPGDGYANTRHNVGFQVASK
                     LAKRARVEFGIKSAESRIAEGALGTSKIAIARPQTFMNDSGKAIRKLLDRYRIEPDRM
                     LVVYDDIDLPLGKIRVRERGGAGTHNGMRSIVARVGEGFTRIRVGVAPADPLAEVGDL
                     AEYVLAPFAADERAEADRVIDRAAEALEVAIRDGLRRAMDRFNG"
     gene            1150..>2021
                     /locus_tag="VFV20_03200"
     CDS             1150..>2021
                     /locus_tag="VFV20_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEU5287385.1"
                     /translation="MRVAVVGFGNVGRALARLLITTSMRPSAALPHPARPLTVTAIGT
                     ARHGAVVAPEGIDLATILAGTDLPLRALPPIRDLPADVLVEITTLEPRTGEPALSYIR
                     DALRADMHVVTANKGPIARAYRELAALAREHGRLLRFEATVADCLPVFTLWRAALPLA
                     RIDAIRGIVSSTCNYVLTQCGGGAELADALREAQRLGLAEADPRNDLEGHDAAAKATI
                     LANVLMDADIRPEDVERHAIDENAADAARAAAAVGARVRPIVDIERRGERIIASFRAR
                     RLEPLDPLYAVDGVS"
BASE COUNT          272 a          762 c          716 g          271 t
ORIGIN      
        1 ggcgcgcagc ggcgcgacga ccgcgatggg ctccgacccc ggcgcggtgt gcatgcgcca
       61 cagcgtcagc agccgctgat ggatggcgcc cgccgatggt gcgccgacct cgaagggcat
      121 cgtctcgcgc tcggggaaca agaggacccg ctcctcgccg agccatcccg cgagctccgc
      181 gcgccagcgg cgcgcgcggt cctcgctcgg cgcgacgagc acgatcggcc gtcgcgcgac
      241 cgcgaccagc gctgaccacg cgaacgcccg cgcgcccgcg gacgtgtcgg cgagcgtgag
      301 cgccgcgcga gtctcgagtc gctcgaccag accggcaaag cgttcgctgc gctcggcgag
      361 cgcgagcacg ccaccgaggc gcgcgccatg ctcctgctcg tcgcgactcg tctccgcccg
      421 ctccgtcgcc gcttccttaa ccattgaacc ggtccatggc acggcgcaga ccgtctcgga
      481 tcgcgacttc gagcgcttcc gcggcacggt cgatgacgcg atcggcctcg gcgcgctcgt
      541 ccgcggcgaa cggcgcgagc acgtactcgg cgaggtcgcc gacctcggcc agcgggtcgg
      601 ccggcgcgac gccgacacgg atgcgcgtga agccctcgcc cacccgcgcg acgatcgagc
      661 gcatcccgtt gtgcgtcccg gcgccgccgc gctcgcgcac gcggatcttc ccgagcggca
      721 ggtcgatgtc gtcgtagacg accagcatcc ggtccggctc gatgcggtag cggtcgagca
      781 gtttccggat cgccttgccg ctgtcgttca tgaaggtctg ggggcgcgcg atcgcgatct
      841 tcgacgtccc gagcgcacct tcggcgatgc gcgactccgc cgacttgatg ccgaactcga
      901 cgcgcgcgcg cttcgcgagc ttgctcgcta cctggaagcc gacgttgtgg cgcgtgttcg
      961 cgtagccgtc acccggattg ccgaggccga cgacgagcag gtccacgcgc gcgggcgcgg
     1021 agcggtcgaa gagcttcgcg agacggacca aggccggctc cttcccttgc aaacgccgaa
     1081 aaggggcgcc accggaatga ccggggtgcc ccgcgagacg cgtattgcgg acaagtctag
     1141 cgtgagagcg tgcgcgtcgc ggtcgttggc ttcggcaacg tcggccgcgc cctcgcgcgg
     1201 ctcctgatca cgacgtcgat gcgtccgtcg gcggcgctgc cgcacccggc tcggccgctc
     1261 accgtgacgg cgatcgggac ggcgcggcac ggcgccgtcg tcgcgccgga gggcatcgac
     1321 ctcgcgacga tcctcgcggg caccgatctc ccgctgcgtg cgctgccgcc gatccgcgac
     1381 cttcccgcgg acgtgctcgt cgagatcacg acgctcgagc cgcgcaccgg cgagcccgcg
     1441 ctctcgtaca tccgggacgc gctccgcgcg gacatgcacg tcgtgaccgc gaacaagggc
     1501 ccgatcgcgc gcgcgtaccg cgagctcgcg gcgctcgcgc gcgagcacgg acggctgctg
     1561 cgcttcgagg cgaccgtcgc cgactgcctg cccgtcttca cgctgtggcg cgcggccctg
     1621 ccgctcgcgc gcatcgacgc gatccgcggg atcgtgtcga gcacctgcaa ctacgtgctc
     1681 acgcaatgtg gcggcggcgc ggagctcgcc gacgcgctgc gggaggcgca gcgcctgggc
     1741 ctcgcggagg ccgatccgcg gaacgacctc gagggccacg atgcggccgc gaaggcgacg
     1801 atcctcgcga atgtcctgat ggatgccgac atccggccgg aggacgtcga gcgccacgcc
     1861 atcgacgaga acgccgcgga tgccgcgcgg gccgccgctg cggtcggcgc gcgcgtacgc
     1921 ccgatcgtcg acatcgaacg gcgcggcgag cgcatcatcg cgtcgttccg cgcccgccgg
     1981 ctcgaaccgc tcgacccgct gtacgccgtg gatggcgtct c
//
LOCUS       DASTZR010000141         2744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_971737, whole genome shotgun sequence.
ACCESSION   DASTZR010000141 DASTZR010000000
VERSION     DASTZR010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971737"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1236)
                     /locus_tag="VFV20_03205"
     CDS             complement(115..1236)
                     /locus_tag="VFV20_03205"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU5287386.1"
                     /translation="MRLLAASLALVLVLTGCGETTRLTSAGSPSASLTATAPAASSAA
                     PAPTASPSQSQTPSPTIDPASCPRGVPCGAIDAYTRVINPYILDGHLGALTSFVSRDP
                     RHPGHAKAKAYIKEQLGALGGGTISSQQTTYQGIPLENIFLTVDPPSPYTFVPQTGGA
                     VLVSAHYDSTANRTSGWRPASDPAPGADDNATGTAGLLELARVVVTARNAGLLRTRVV
                     LAFFDGEELTFKGSGAYMATLSKPYPYKAAINLDMVGFNPLADRLDLLWYTNASAGLR
                     DRVIRANETYRIGVAPLNAQYAADGSTIMDAAPFGVAGIPSIALCQRYGENDATFPGN
                     YTFHTVTDTPDKITNKRLWLKATRLTLATALELASETAP"
     gene            complement(1333..2097)
                     /locus_tag="VFV20_03210"
     CDS             complement(1333..2097)
                     /locus_tag="VFV20_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU5287387.1"
                     /translation="MDITTFFQEHGLRPATWPMFADPAIWRFFGEGLRETVFISLVSV
                     VLSFIVGLLFALGRLSRKFWIRWPSIAYIEMIRALPVLLLIVYFGIKGSSLFRPIFGE
                     DFELPRFWAGVLGLSMYTSAVLAEIIRAGIVSLPRGQTEASRALGLSHASTMRFVLLP
                     QALRLMVPAMMAQLTTIIKDTSLVGTIGVFELLKQGRTIYTQYFNPIEVLLIVSVIYF
                     VICFTLSQASRRLELGWAPREVRVKIQEVQLRDQAA"
     gene            complement(2097..>2744)
                     /locus_tag="VFV20_03215"
     CDS             complement(2097..>2744)
                     /locus_tag="VFV20_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU5287388.1"
                     /translation="EFPEQFFGGLWFTVQISIFGLIGSLVLGTVIALCRVSPVAVLRW
                     LGGAYVEFFRNTPLVVQLFFLFLGLPLTGFRFATDTFDNIFRAAVAGMALYHAAYVAE
                     IVRGGLLGVDKGQIEAARTLGLSYAQMLRYVQLPQTFRSVIPPLGNIAIALVKNTSLA
                     STIGVAELLFAAEFVETRTFRAEEAFLTSTMLYLLLTIPMGVVVNALERRMLIER"
BASE COUNT          483 a          887 c          990 g          384 t
ORIGIN      
        1 cgtactcggc gtccgacagc ggcgcgtcca ccggacgcag cagcacgaac gcgagcgcga
       61 agacgagcac cagtccaccg gccgcgagca gcgtcgcgcg gcggccgacg cccgtcacgg
      121 cgccgtctcg ctcgcgagct cgagcgcggt cgccagggtg agcctcgtgg ccttcagcca
      181 cagccgcttg ttcgtgatct tgtcgggtgt gtccgtcacc gtgtggaagg tgtagttccc
      241 ggggaacgtc gcatcgttct caccataccg ctggcacagc gcgatgctcg ggatgccggc
      301 gacgccgaac ggggcggcat ccatgatcgt gctcccgtcg gccgcgtact gcgcgttgag
      361 cggcgcgacg ccgatccggt acgtctcgtt cgccctgatc acgcggtcgc gcagccccgc
      421 ggaggcattc gtgtaccaga gcaggtccag cctgtcggcg agcgggttga agccgaccat
      481 gtcgaggttg atcgcggcct tgtagggata cggcttcgac agggtcgcca tgtacgcacc
      541 gctgcctttg aaggtcagct cctcgccgtc gaagaacgcg aggacgacgc gggtcctcag
      601 cagccccgcg ttgcgcgcgg tcacgacgac ccgcgcgagc tcgagcaggc ctgccgtccc
      661 ggtcgcgttg tcgtccgcgc ccggcgcggg atcgctcgcc gggcgccagc cgctggtgcg
      721 attggcggtc gagtcgtagt gagccgacac caggacggca ccgcccgtct gcggcacgaa
      781 ggtgtacggc gacggcggat cgacggtcag gaagatgttc tcgagcggga tgccctgata
      841 ggtcgtctgt tgcgacgaga tcgtcccccc gccgagcgcg ccgagctgtt ccttgatata
      901 ggccttcgcc ttcgcgtgac ccggatggcg agggtcacgc gacacgaagc tcgtcagtgc
      961 gcccaggtgc ccatcgagga tgtacggatt gatcacccgc gtgtacgcgt cgatcgcgcc
     1021 gcagggcaca ccgcgcgggc agctcgccgg gtcgatggtc ggcgacggcg tctggctctg
     1081 cgacggcgac gcggtcggtg cgggcgcggc gctcgacgcc gcgggtgcgg tcgccgtgag
     1141 cgacgcggac ggagagccgg ccgaggtgag gcgcgtggtc tccccgcagc cggtcaggac
     1201 gagcacgagg gcgagggagg ctgcgaggag acgcatcctt ctcaggctag cgccggatgg
     1261 accgcgtgcc agggcagcga ccgtgcacga cgcgaacaac gagccggccg gacgaatggt
     1321 tcgcgacggc gctcaggcgg cctggtcgcg cagctggacc tcctggatct tcacgcggac
     1381 ctcgcgcggc gcccatccga gctcgagccg gcggctcgcc tgcgacaggg tgaagcagat
     1441 cacgaagtag atcaccgaca cgatgagcag gacctcgatc ggattgaagt actgcgtgta
     1501 gatggtgcgg ccctgcttga gcagctcgaa gacgccgatc gtgccgacga gggaggtgtc
     1561 tttgatgatg gtcgtgagct gcgccatcat cgccgggacc atcaggcgca gggcctgcgg
     1621 gagcagcacg aagcgcatcg tgctcgcgtg ggacaggccg agcgcgcgcg aggcctcggt
     1681 ctggccccgc ggcaggctga cgatgccggc gcggatgatc tcggcgagca cggcgctggt
     1741 gtacatcgag agcccgagca cccccgccca gaagcgcggg agctcgaagt cttcaccgaa
     1801 gatcgggcgg aagaggctcg agcccttgat cccgaagtac acgatgagga gcagcaccgg
     1861 gagcgctcgg atcatctcga tgtaggcgat cgacggccac cggatccaga acttgcgcga
     1921 cagacggccc agcgcgaaca gcagcccgac gatgaacgaa aggacgacgg agacgagcga
     1981 gatgaagacc gtctcgcgga gcccctcgcc gaagaatcgc cagatcgccg ggtccgcgaa
     2041 catcggccac gtcgccggcc gcagcccgtg ctcctggaag aacgtggtga tgtccatcag
     2101 cgctcgatga gcatccgccg ctcgagcgcg ttcaccacga cgcccatcgg gatggtcagc
     2161 agcaggtaca gcatggtcga ggtgaggaac gcctcctccg cgcggaacgt gcgggtctcg
     2221 acgaactcgg cggcgaacag cagctccgcg acgccgatcg tcgatgcgag cgaggtgttc
     2281 ttgacgagcg cgatggcgat gttgccgagc ggtggaatga cgctgcggaa ggtctgcgga
     2341 agctgcacgt accggagcat ctgcgcgtag gagagcccca gcgtccgtgc cgcctcgatc
     2401 tgccccttgt ccacgccgag caggccaccg cgtacgatct cggcgacgta ggcggcgtgg
     2461 tacagcgcca tcccggcgac cgcggcgcgg aagatgttgt cgaaggtgtc ggtcgcgaag
     2521 cggaaaccgg tgagcggcag cccgaggaac aggaagaaga gctgcacgac cagcggggta
     2581 ttgcggaaga actccacgta tgccccgccg agccaccgca ggaccgcgac cggcgagacc
     2641 cggcacaggg cgatgacggt gccgagcacg agcgaaccga tgaggccgaa gatggagatc
     2701 tgcaccgtga accagagccc gccgaagaac tgctcgggga actc
//
LOCUS       DASTZR010000142         2400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_790384, whole genome shotgun sequence.
ACCESSION   DASTZR010000142 DASTZR010000000
VERSION     DASTZR010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790384"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VFV20_03220"
     CDS             complement(<1..380)
                     /locus_tag="VFV20_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5287389.1"
                     /translation="MKQRSWSDVALTVWGVAVFAFLFLPIAVIIVSSFNTGRLLVAWD
                     GFGFDAFTALVAKPAIRDAVLVSIQTGLIAAVLATLLGTLAGIAMARHPGRWAYWFIG
                     LLLLVSVTPEIVDAVALLPWLVFLG"
     gene            complement(377..1324)
                     /locus_tag="VFV20_03225"
     CDS             complement(377..1324)
                     /locus_tag="VFV20_03225"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5287390.1"
                     /translation="MPRSALAIPAWAWLVVFFVAPVAMVVYFSFGYKPGIFGTHATDV
                     LSFDRYAEALSPTFFQTFANTLWVGIAGTLLCLLIGLPVAYWMAVKVPASRRGVLLAL
                     VMVPFWTNFLVRTIGWQVILAPEGWLSTLLQGIGVTDGPLEVLYTRTAVLIGVVYNYL
                     PLMILPLFVAFDRVGGPLREASKDLGAGRVRTFLRVTLPLARPGVIAGVLLVFIPLMG
                     DYITATVLGGARGNMVGQLVASQFQTAQNWALGSAMAVLLILVIMTTVAIGGVVVWLV
                     SLPFRMRNRLVLGDAPGAAEPPEAATPAEPARSPEEVAA"
     gene            complement(1339..>2400)
                     /locus_tag="VFV20_03230"
     CDS             complement(1339..>2400)
                     /locus_tag="VFV20_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU5287391.1"
                     /translation="LAACAPGGGGAPKPQATGGALEDSLSVYTWGDYDSPDVVSGFQS
                     ELGPKVTLDSYSSNEELIAKLIAAKGTSGYDIVVPTGVFVPQMIENGLLSKLNRDLIP
                     NLQHVDDAYLGRAWDPENDYSICKAWGTTGFVYDTTVITRELSTWADFLDAAQNEASG
                     KTSVLDDPGDLTGMYFWANGIDWNTTDEADLAAAEDFIVNTLAPHVSAFDSYPGGAAI
                     PQGTHALMQAWNGDARIGILESADPDRWKWVFPGPETEIWMDNWAIAAGAPHPEAAHA
                     FIDHVLTPENSLAELDYIGYHTGGKDIEQAAADAGLPMLDLVFFTPEQLETMHEGEVN
                     EAQQVRVDIWNKAKAAAGA"
BASE COUNT          391 a          834 c          844 g          331 t
ORIGIN      
        1 ccgaggaaca cgagccacgg cagcagggcg accgcgtcga cgatctcggg cgtgaccgac
       61 acgagcagca gcaggccgat gaaccagtag gcccaccggc ccggatggcg cgccatcgcg
      121 atgcccgcga gcgtgccgag cagcgtcgcg agcaccgccg cgatgaggcc cgtctggatc
      181 gagacgagca ccgcgtcgcg gatcgcgggc ttggcgacga gcgcggtgaa cgcgtcgaac
      241 ccgaagccgt cccaggcgac gaggagccgc ccggtgttga acgacgacac gatgatcacc
      301 gcgatgggca ggaacaggaa cgcgaacacc gcgacgcccc acacggtgag cgcgacatcc
      361 gaccacgacc gctgcttcac gcggccacct cctcgggact gcgggcgggt tcggccggcg
      421 tcgcggcctc gggcggctcg gccgcccccg gcgcgtcgcc caggaccagg cgattgcgca
      481 tccggaacgg caaggacacc agccacacga cgaccccgcc gatcgcgacg gtcgtcatga
      541 tcacgaggat cagcagcacc gccatggccg agccgagcgc ccagttctgg gcggtctgga
      601 actggctcgc gacgagctgg cccaccatgt tgccgcgtgc gccgccgagc acggtcgcgg
      661 tgatgtagtc gcccatgagc gggatgaaca cgaggagcac gcccgcgatc acaccgggcc
      721 gcgcgagcgg cagcgtgacg cgcaggaacg tgcggacgcg tccggcgccg aggtccttcg
      781 acgcctcgcg gagcggtccg ccgacgcggt cgaacgcgac gaacagcggc aggatcatga
      841 gcgggaggta gttgtacacc accccgatga gcaccgccgt ccgcgtgtag agcacctcca
      901 gcggtccatc cgtcaccccg atgccctgca gcagcgtcga cagccagccc tcgggcgcga
      961 ggatcacctg ccagccgatc gtgcggacca ggaagttggt ccagaagggc accatgacga
     1021 gcgcgagcag cacgccgcgt cgcgacgccg gcaccttcac cgccatccag tacgccacgg
     1081 gcaggccgat gagcaggcac agcagcgttc ccgcgatgcc gacccacagc gtgttcgcga
     1141 aggtctggaa gaacgtcggc gacagcgcct cggcgtaccg gtcgaacgac agcacgtcgg
     1201 tcgcgtgcgt gccgaagatg cccggcttgt agccgaagct gaagtacacg accatcgcca
     1261 ccggggcgac gaagaagacc accagccacg cccacgcggg gatcgcgagg gcggagcgcg
     1321 gcatccggag cgctcgcact acgcccccgc cgcggccttc gccttgttcc agatgtcgac
     1381 gcgcacctgc tgtgcctcgt tcacttcgcc ctcgtgcatg gtctcgagct gctcgggcgt
     1441 gaagaacacc aggtcgagca tcggcaggcc ggcgtcggcc gcggcctgct cgatgtcctt
     1501 gccgcccgtg tggtagccga tgtagtcgag ctcggccagc gagttctcgg gcgtgagcac
     1561 gtgatcgatg aatgcgtgcg cggcctcggg gtggggcgcg cctgcggcga tcgcccagtt
     1621 gtccatccag atctcggtct cgggtccggg gaacacccac ttccagcggt ccggatcggc
     1681 cgactccagg atcccgatgc gcgcgtcgcc gttccacgcc tgcatgagcg cgtgcgtgcc
     1741 ctgcgggatc gccgcgccgc cggggtagga gtcgaatgcc gagacgtgcg gggcgagcgt
     1801 gttgacgatg aagtcctcgg cggccgcgag gtcggcctcg tccgtagtgt tccagtcgat
     1861 gccgttggcc cagaagtaca tgcccgtgag gtcgcccggg tcgtcgagca cgctcgtctt
     1921 cccgctggcc tcgttctgcg ccgcgtcgag gaagtcggcc caggtcgaca gctcgcgcgt
     1981 gatcacggtc gtgtcgtaca cgaacccggt cgtgccccac gccttgcaga tcgagtagtc
     2041 gttctcgggg tcccaggccc ggccgaggta cgcgtcgtcc acgtgctgca ggttggggat
     2101 caggtcgcgg ttgagcttgc tgagcagccc gttctcgatc atctgcggga cgaacacccc
     2161 ggtcggcacg acgatgtcgt agcccgaggt gcccttcgcg gcgatgagct tcgcgatgag
     2221 ctcctcgttc gagctgtagg agtcgagcgt gaccttgggg ccgagctccg actggaagcc
     2281 cgagacgacg tcgggcgagt cgtagtcgcc ccacgtgtac accgagaggc tgtcctcgag
     2341 cgcgccgccg gtggcctgcg gcttcggggc gccgccgccg ccgggcgcgc aggcggccag
//
LOCUS       DASTZR010000143         2501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_371958, whole genome shotgun sequence.
ACCESSION   DASTZR010000143 DASTZR010000000
VERSION     DASTZR010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371958"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1757
                     /locus_tag="VFV20_03235"
     CDS             <1..1757
                     /locus_tag="VFV20_03235"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEU5287392.1"
                     /translation="RGRIVMRAKAHTEEMRGNREAIIVTELPYAVNKASLIEKIADLV
                     QNKRLAGISDIRDESDREGMRVVIEVKRDDSANRVLNNLFKHTAMQSTFNLNMLALVD
                     EQPRVLSMKEVLQHHIDYRRSVIRRRTEYDLKKAKERAHILEGLKIAHANLDALIKLI
                     RAHRGQESLLIGKMTEQFGLSEIQAKAILDMQLRRLSALEREKLDEEYETTIKLIAEL
                     ESVLASPRKISGMIKADLEALAEKYGDERRTEIVNDLPGQFTDEQLVADEDVVITLSS
                     RNYAKRMPLSTYRQQHRGGRGVIGMQTRDEDDVEHLVVARNHDRLYVFTDRGRVFALK
                     AYELPDASRTAKGTPIQNILEAMQQGERVSALVALRDTSTTEFLVMATRKGFLKKTSL
                     KEYASVRRAGLIAIALRKGDELAWVAPCGKDDRILLATKKGKGITFKASDARPMGRQT
                     QGVTGIRLAKGDEVVGMGVVRPRTEVLSVTANGYGKRTAVDEFPTQGRGGQGVILASL
                     GPKTGDVAAAQIVDEGTQEILLISTTGVVIRTPLDQIRVLGRATMGVKVMTTGDAKIA
                     SIATFTTTRPTQTQLPVR"
     gene            1990..>2501
                     /locus_tag="VFV20_03240"
     CDS             1990..>2501
                     /locus_tag="VFV20_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5287393.1"
                     /translation="MITLDDVSKRYPNGTFALRDVDLQIEAGDFVFLVGSSGAGKSTL
                     IRLLIREDMPTTGRVLVDGKDVAKLSRGNVSKLRRKVGVVFQDFKLLPAKTVAENVAF
                     ALIVTGHEGRVVREETDRVLSLVGLSQRRDHFPDQLSGGEQQRTAIARALVNRPQILI
                     ADEPTGNLDPV"
BASE COUNT          463 a          859 c          823 g          356 t
ORIGIN      
        1 gccgcgggcg catcgtcatg cgcgcgaagg cccacaccga ggagatgcgc gggaaccgtg
       61 aggcgatcat cgtcaccgag ctcccgtacg ccgtgaacaa ggcgtcgctc atcgagaaga
      121 tcgccgacct ggtgcagaac aagcgcctcg cgggcatcag cgacatccgg gacgagagcg
      181 accgcgaggg catgcgcgtg gtcatcgagg tgaagcggga cgactcggcg aatcgcgtgc
      241 tgaacaacct cttcaagcac accgccatgc agtcgacctt caacctcaac atgctcgccc
      301 tcgtcgacga gcagccgcgg gtcctctcga tgaaggaggt cctgcagcat cacatcgact
      361 accgccgcag cgtcatccgc cgccgcaccg agtacgacct gaagaaggcg aaagagcgcg
      421 cgcacatcct cgagggcctc aagatcgcgc acgcgaacct cgacgcgctc atcaagctca
      481 tccgcgcgca ccgcggccag gaatcgctcc tcatcggcaa gatgaccgaa cagttcggcc
      541 tgtccgagat ccaggcgaag gcgatcctcg acatgcagct gcggcgcctc tcggcgctgg
      601 agcgcgagaa gctcgacgag gagtacgaga cgaccatcaa gctcatcgcc gagctcgaat
      661 cggtcctggc gtcgccgcgg aagatcagcg gcatgatcaa ggcggacctg gaggcgctcg
      721 ccgagaagta cggcgacgag cgccgcaccg agatcgtgaa cgacctgccg ggccagttca
      781 ccgacgagca gctggtcgcc gacgaggacg tcgtgatcac cctctcgagc cgcaactacg
      841 ccaagcgcat gcccctctcg acctaccgcc agcagcaccg cggcggccgc ggcgtcatcg
      901 gcatgcagac gcgcgacgag gacgacgtcg agcatctcgt cgtcgcccgc aaccacgacc
      961 gtctctacgt gttcaccgat cgcggccgcg tgttcgcgct gaaggcgtac gagctgccgg
     1021 acgcgagccg caccgcgaag ggcacgccga tccagaacat cctcgaggcg atgcagcagg
     1081 gcgagcgcgt ctccgcgctc gtcgcgctcc gtgacacctc caccaccgag ttcctcgtga
     1141 tggcgacgcg caagggcttc ctcaagaaga cctcgctcaa ggaatacgcg agcgtccggc
     1201 gggccgggct catcgccatc gcgctccgca agggcgacga gctcgcgtgg gtcgcaccgt
     1261 gcggcaagga cgaccgcatc ctcctcgcga cgaagaaggg caagggcatc accttcaagg
     1321 cgagcgacgc gcggccgatg ggccgccaga cgcagggcgt caccggcatc cgtctcgcga
     1381 agggcgacga ggtcgtcggt atgggcgtgg tccggccgcg gaccgaggtg ctctcggtca
     1441 ccgcgaacgg ctatgggaag cgcacggccg tcgacgagtt cccgacgcag ggccgcggcg
     1501 gccagggcgt catcctcgcc tcgctcggtc cgaagaccgg cgacgtcgcg gcggcgcaga
     1561 tcgtcgacga gggcacgcag gagatcctgc tcatcagcac gacgggcgtc gtgatccgca
     1621 cgccgctcga ccagatccgc gtgctcggca gggccaccat gggtgtgaag gtgatgacga
     1681 ccggggacgc gaagatcgcc tcgatcgcca cgttcaccac gacccggccg acccagacgc
     1741 agctaccggt ccgctgaccg ccgagcggcc cggcgtcggc aggcaggctg gcacgcgtgc
     1801 cggcccgtcc gccgagcgta cgtttttgtg gcaagcgagc gccccctagt accaattcgc
     1861 cacattcggg cgttgtgttc gacgcggacg ggcgcgtatg gtccgcagca ggtcaagttc
     1921 gcatgggttc gcttgttcgg gtttcgaata ggcgcgggat cgacacgaga caagggaagg
     1981 gttgccgtat tgatcacgct cgacgacgtc agcaagcgat atccgaacgg gaccttcgcg
     2041 ctgcgcgacg tcgacctgca gatcgaagcc ggtgatttcg tgttcctcgt gggctcatcc
     2101 ggcgccggga agtccaccct catccgcctt ctcatacgcg aggacatgcc cacgaccggc
     2161 cgcgtgctgg tggacgggaa ggacgtcgcg aagctctccc gtggcaacgt ctcgaagctg
     2221 cggcggaagg tcggcgtcgt gttccaggac ttcaagctgc tgccggcgaa gaccgtcgcg
     2281 gagaacgtcg cgttcgcgct catcgtcacc ggacacgagg gccgcgtcgt gcgcgaggag
     2341 accgatcgcg tcctctcgct cgtcggtctc tcgcagcggc gcgatcactt cccggatcaa
     2401 ctttccggtg gcgagcagca gcgcaccgcg atcgcgcgcg cgctcgtcaa tcgtccgcag
     2461 atcctcatcg ccgatgaacc gaccggcaac ctcgatccgg t
//
LOCUS       DASTZR010000144         4913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1078869, whole genome shotgun sequence.
ACCESSION   DASTZR010000144 DASTZR010000000
VERSION     DASTZR010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078869"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..338
                     /locus_tag="VFV20_03245"
                     /pseudo
     CDS             1..338
                     /locus_tag="VFV20_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636767.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
     gene            complement(275..1555)
                     /gene="atpD"
                     /locus_tag="VFV20_03250"
     CDS             complement(275..1555)
                     /gene="atpD"
                     /locus_tag="VFV20_03250"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU5287394.1"
                     /translation="MATKEGTVTGEIVQIIGPVLDVRFEEGHLPEIYHALSVKKDDGS
                     LVIAEVQQHLGNNWVRAVSMQSTDGLRRGMKATNTGGPITVPVGKGTLGRIFNVVGEP
                     IDGLGKVEAVRRDPIHRQPPAFTEQSTTAEIFETGMKVIDLICPFLKGGKSAVFGGAG
                     VGKTVVIQELIRNIAAEHGGYSVFCGVGERSREGTQLLGEMKESGVIDKMSMVFGQMN
                     EPPGARLRVALTGLTIAEYFRDEEGRDLLIFIDNIFRFTQAGSEVSALLGRMPSAVGY
                     QPTLATEMGELQERITSTKKGSITSLQAVYVPADDYTDPAPATTFAHLDSSIRLERYL
                     TELNLYPAVDPLTSTSRALDPQIVGQDHYDTAREVQRVLQRYKDLQDIIAILGVDELS
                     DDDKVLVARARKIQRSRRIVESRCPNVVAGAGSV"
     gene            complement(1566..2426)
                     /gene="atpG"
                     /locus_tag="VFV20_03255"
     CDS             complement(1566..2426)
                     /gene="atpG"
                     /locus_tag="VFV20_03255"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEU5287395.1"
                     /translation="MPSERDLRRRARSVKNIQQLTRAMQLVAASKMRRAQESVLASRP
                     FEEKLRTVLNDLAPYADPELSPLLQRRTPVKKVAVVLITTDRGLVGPMNVNLLRTALR
                     FAEQQPSVEYVAVGRKGIGSLRRMRMPVIAEFPGIPDRPTTADTNPIARTVVDEFTSG
                     NVDEVHLGFTRFINTLRQEPTMRRLLPLVPEEEDLDKLPPIQYLFEPDPQTVMDEILP
                     RFVELAMYQAILESKASQFSAQMVAMRNATDAADDLISELTLAANKARQTRITKEMLE
                     IASGAEALKG"
     gene            complement(2514..4025)
                     /gene="atpA"
                     /locus_tag="VFV20_03260"
     CDS             complement(2514..4025)
                     /gene="atpA"
                     /locus_tag="VFV20_03260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU5287396.1"
                     /translation="MATTSSEITDIIKSQIQRFEGKPESVDVGTVVEIGDGIARIHGL
                     ANCMASELLDFGHDVFGLAFNLEEDTVSAIILGEYTGIEEGGEVKTTGRIVEAAVGPQ
                     LVGRVVDPLGRPLDGKGPIDTKEFRPVDVVAPGVITRQNVDTPVQTGIKVIDAITPIG
                     RGQRELIIGDRQTGKTAVAIDTIINQKGKDLTCIYVAIGQKESSVAKIVATLEEHGAM
                     EHTIVVIAGASSPATLQYLAPFGGCAMGEYFRDAGKDALVVYDDLSKHAWAYRQVSLL
                     TRRPPGREAYPGDVFYLHSRLLERAARLNTENGGGSLTALPIIETQANDISAYIPTNV
                     ISITDGQIYLETDLFNQGVRPPMNTGLSVSRVGGDAQTKAMKAVVKGLKADIAQYREL
                     AAFAQFGSDLDKATQQQLRRGEKTVEIMKQPQYQPLPLEKEVVIIFAAGNGYIDDVPT
                     PKVREFERDLFRFMDTQAKALGDRIAKDKKFDAEIEKQVRAMLDDFKKTVTYS"
     gene            complement(4039..4569)
                     /gene="atpH"
                     /locus_tag="VFV20_03265"
     CDS             complement(4039..4569)
                     /gene="atpH"
                     /locus_tag="VFV20_03265"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEU5287397.1"
                     /translation="MALSGSAGRRYAEALIDLAGDPATVEAYRASLERLARAIGPSSI
                     ALLRDPRVSLDHRRDALSGAVKDEPPVIRSLLVLLLERQRIHLLPDIARFYADTVDRR
                     AGIEKAKITTPIELSAADRDDLVRRLERSSGRKLKATFLVDPSLLGGAKVQIGDHVID
                     TSLASQLQEMARELAG"
     gene            complement(4569..>4913)
                     /locus_tag="VFV20_03270"
     CDS             complement(4569..>4913)
                     /locus_tag="VFV20_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HEU5287398.1"
                     /translation="SAKQERERMKQEVEQLLAQARREAGELAERTRTAAEAAAADIKA
                     QAKVEADRTREKGRADAQALHDQALAQLRTEVASMVVLAASRILGREVSPEQHRALIE
                     RSLDEAGGELKH"
BASE COUNT          698 a         1647 c         1690 g          878 t
ORIGIN      
        1 cccccggcgc ccgcctccgg gtcggcctgt cgggcctcac gatggccgag tacttccgcg
       61 atgagcgaaa ccaggacgtg ctgctcttca tcgacaacat cttccggttc acccaggcag
      121 gctccgaggt ctccacgctg ctcggccgca tgccgagcgc cgtgggctac cagcccaccc
      181 tcgcgaccga gatgggccag ctccaggagc ggatcacgtc gacctcgaag ggctcgatca
      241 cgtcgctcca ggccgtcttc gtgccggccg atgactacac cgaccccgcc ccggccacca
      301 cgttcggaca tctcgactcc acgatccgcc tggagcgctg gatcttgcgc gcacgggcga
      361 cgaggacctt gtcgtcgtcg gagagctcgt cgacgccgag gatggcgatg atgtcctgca
      421 ggtccttgta ccgctgcagc acgcgctgca cttcgcgcgc ggtgtcgtag tggtcctgac
      481 ccacgatctg cggatcgagc gcgcggctcg tggaggtgag cgggtcgacc gccggataga
      541 ggttcagctc ggtgaggtac cgctcgaggc ggatcgacga gtccaggtgc gcgaacgtgg
      601 tggccggcgc cgggtcggtg tagtcgtcgg ccggcacgta cacggcctgc agcgacgtga
      661 tcgacccctt cttcgtggag gtgatccgct cctgcagctc gcccatctcg gtcgcgaggg
      721 tcggctggta gcccacggcg ctcggcatcc ggccgaggag cgcggacacc tcggacccgg
      781 cctgcgtgaa ccggaagatg ttgtcgatga agatgaggag gtcgcgcccc tcctcgtcgc
      841 ggaagtactc ggcgatggtg aggccggtga gcgcgacgcg caggcgtgct ccgggcggct
      901 cgttcatctg gccgaagacc atcgacatct tgtcgatgac gcccgactcc ttcatctcgc
      961 ccagcagctg cgtgccctcg cgcgagcgct cgccgacgcc gcagaagacg gagtagccac
     1021 cgtgttccgc ggcgatgttg cggatgagct cctgaatgac gacggtcttg ccgacgccgg
     1081 cgccgccgaa gaccgccgac ttgccaccct tgaggaacgg gcagatgagg tcgatgacct
     1141 tcatgcccgt ctcgaagatc tccgcggtgg tggactgctc ggtgaaggcg gggggctggc
     1201 ggtggatcgg gtcgcgccgc accgcttcca ccttgccgag gccgtcgatg ggctcgccga
     1261 cgacgttgaa gatgcggccg agggtgccct tcccgacggg cacggtgatc ggtccacccg
     1321 tgttggtcgc cttcatgccg cggcggagtc cgtcggtgct ctgcatcgag acggcgcgca
     1381 cccagttgtt gcccaggtgc tgctgcacct ccgcgatcac cagggatccg tcgtccttct
     1441 tcaccgagag cgcgtggtag atctcgggca ggtggccctc ttcgaaccgc acgtcaagga
     1501 ccggtccgat gatctggacg atctcgccgg tgacggtgcc ctctttcgtc gccactgcca
     1561 tttccttacc ccttcagcgc ttcggcgccc gaagcgatct ccagcatttc cttggtgatg
     1621 cgtgtctgcc gcgccttgtt cgccgcgagc gtcagctccg agatgaggtc gtcggcggca
     1681 tcggtcgcgt tgcgcatcgc gaccatctgc gccgagaact gcgaggcctt cgattcgagg
     1741 atcgcctggt acatcgcgag ctcgacgaac cgcgggagga tctcgtccat gaccgtctgc
     1801 ggatccggct cgaagaggta ttggatcggc ggaagcttgt cgaggtcctc ctcctcgggc
     1861 acgagcggca gcaggcggcg catcgtcggc tcctggcgca gggtgttgat gaaccgcgtg
     1921 aagccgagat gcacctcatc gacgttgccg ctggtgaact cgtcgaccac ggtgcgtgcg
     1981 atcgggttcg tgtcggccgt cgtgggccgg tccgggatgc ccgggaactc ggcgatgacc
     2041 ggcatgcgca tgcggcgcag ggagccgatg cccttccgac cgaccgcgac gtattcgacc
     2101 gacggctgct gctcggcgaa ccgcagcgcg gtgcggagca ggttcacgtt catcggaccc
     2161 acaagaccgc ggtcggtcgt gatgagcacg accgcgacct tcttcaccgg cgtgcgccgc
     2221 tggagcagcg ggctgagctc ggggtcggca tacggcgcga ggtcgttcag gaccgtgcgc
     2281 agcttctcct cgaacgggcg cgaggcgagc accgactcct gggcacggcg catcttcgac
     2341 gcggccacga gctgcatcgc ccgcgtgagc tgctggatgt tcttcaccga ccgcgcgcgg
     2401 cggcgaagat cgcgctcgct gggcatctag ttcgatctcc ggctgcgccg gagacacgcc
     2461 ccggcgggat gactcgctcg tcgcggtaca gccgctcctc gctcgacttt catctagctg
     2521 tacgtcaccg tcttcttgaa gtcgtcgagc atggcgcgca cctgcttctc gatctcggcg
     2581 tcgaacttct tgtccttggc gatgcgatcg ccgagcgcct tcgcctgcgt gtccatgaag
     2641 cggaagaggt cgcgctcgaa ctcgcgcacc ttcggcgtcg gcacgtcgtc gatgtatccg
     2701 ttgccggcgg cgaagatgat cacgacctcc ttctcgaggg gcaggggctg gtactgcggc
     2761 tgcttcatga tctcgaccgt cttctcgccg cggcgcagct gctgctgcgt ggccttgtcg
     2821 aggtcggagc cgaactgcgc gaacgcggcg agctcgcggt actgcgcgat gtcggccttc
     2881 aggcccttca ccacggcctt catcgccttc gtctgggcgt cgccaccgac acgggagacg
     2941 gacagaccgg tgttcatcgg cggacggacg ccctggttga acaggtccgt ctcgaggtag
     3001 atctggccgt cggtgatcga gatgacgttc gtcgggatgt acgcggagat gtcgttcgcc
     3061 tgcgtctcga tgatcggaag cgcggtcagc gagccaccgc cgttctccgt gttcaggcgg
     3121 gccgcgcgct cgagcaggcg cgagtgcagg tagaagacgt caccgggata cgcctcgcgg
     3181 cccggaggcc ggcgggtgag aagcgacacc tggcggtacg cccaggcgtg cttcgacagg
     3241 tcgtcgtaca cgacgagcgc gtccttgccg gcgtcgcgga agtactcgcc catcgcgcag
     3301 ccgccgaacg gcgcgaggta ctgcagggtc gcgggagacg acgcgccggc gatgacgacg
     3361 atggtgtgct ccatcgcgcc gtgctcctcg agcgtcgcga cgatcttcgc gacggacgac
     3421 tccttctgtc cgatcgcgac gtagatgcag gtgaggtcct tccccttctg gttgatgatc
     3481 gtgtcgatcg cgacggcggt cttccccgtc tggcggtcgc cgatgatcag ctcgcgctgg
     3541 ccgcggccga tcggcgtgat cgcgtcgatc accttgatgc ccgtctgcac cggcgtgtcc
     3601 acgttctgac gtgtgatgac gcccggcgcc acgacgtcga cggggcggaa ctccttcgtg
     3661 tcgatcggtc ccttgccgtc gagcggccgc ccgagcgggt cgaccacgcg gcccacgagc
     3721 tgcgggccga cggcggcctc cacgatgcgg ccggtcgtct tgacctcgcc gccctcttcg
     3781 atgccggtgt actcgcccag gatgatcgcg ctcaccgtgt cttcctcgag gttgaaggcg
     3841 aggccgaaga cgtcgtgccc gaaatccagg agctcggagg ccatgcagtt cgcgaggccg
     3901 tggatccgcg cgatgccgtc gccgatctcg acgacggtgc cgacgtccac ggactcgggc
     3961 ttgccctcga agcgctggat ctgcgacttg atgatgtcgg tgatctctga cgacgtcgtg
     4021 gccatgtctt ctccttacct acccggcgag ctcgcgcgcc atctcctgga gttgcgacgc
     4081 gagcgatgtg tcgatgacgt gatcgccgat ctggaccttc gccccaccga ggagcgacgg
     4141 atcgacgagg aaggtcgcct tgagcttccg tccgctggac cgttcgagac gtcgcaccag
     4201 gtcgtcgcgg tcagcggcgg acagctcgat gggcgtggtg atcttcgcct tctcgatgcc
     4261 ggcgcggcgg tcgacggtgt cggcatagaa ccgcgcgatg tcgggcagca gatggatgcg
     4321 ctggcgctcc agcagcagca cgaggagcga gcggatgacg ggcggctcgt ccttgacggc
     4381 gccggagagc gcgtcgcggc gatgatcgag ggacacgcgg ggatcgcgga gcagcgcgat
     4441 gctcgacgga ccgatggcgc gcgcgagacg ctcgagcgac gctcgatatg cctcgacggt
     4501 cgcgggatca cccgcgaggt cgatgagcgc ctcggcataa cggcggccgg ccgatccgga
     4561 cagcgccact agtgcttcag ctccccgccg gcctcatcga gcgatcgctc gatgagcgcg
     4621 cggtgctgct cgggactgac ctcgcgcccg aggatgcggc tcgcggcgag gacgaccatc
     4681 gacgcgacct cggtgcgcag ctgcgcgagc gcctggtcgt ggagcgcctg ggcgtcggcg
     4741 cgacccttct cgcgggtccg gtccgcctcc accttcgcct gcgccttgat gtccgcggcc
     4801 gccgcttcgg ccgccgtgcg cgtgcgctcg gcgagctcgc ctgcctcacg acgcgcctgg
     4861 gcgaggagct gctcgacctc ctgcttcatc cgctcgcgtt cctgcttcgc gga
//
LOCUS       DASTZR010000145         2855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_319719, whole genome shotgun sequence.
ACCESSION   DASTZR010000145 DASTZR010000000
VERSION     DASTZR010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319719"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="VFV20_03275"
     CDS             <1..1073
                     /locus_tag="VFV20_03275"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HEU5287399.1"
                     /translation="GPARTGGIEMPSIIRVRRRVLALLAGTLLVAFSAAPALACGGLI
                     GPNGAVNLLRTTTFAGYHAGEEHYVTAFQFAGGGGAFGSITPLPGIPTSVVKGGDWTL
                     QRLVRETNPVPEAAVFASDSVRAGAVELMKVKIDALDITVLKGGAADIGTWASAHGFR
                     LPPDAPEVLDFYAARSPIFMAAVFDADAAKARGQRIGDGTPVHLVIPTPSPWVPLRIL
                     GLGKTAQDRVDADVYLLTDIKPQLLPAPLGVNGIRIDHSATATDSLLNDLRSDKGMSW
                     VPSSGWLTKIVVDASAGQLRYDLAVSTTAAVPSPVSAGLEAPTAPLRSQTTELTGFVM
                     AGIVLIGLGGLLILRTPAPARV"
     gene            1070..1477
                     /locus_tag="VFV20_03280"
     CDS             1070..1477
                     /locus_tag="VFV20_03280"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU5287400.1"
                     /translation="MTARLIVLALAASALWAGAACGTAQASTVRQVEIRIHYSKFEPA
                     TVSVPAGVPVTFVIRNLDPIDHEWLIGDAAFHARHRSGTEPVHGERADEVSLPAGATR
                     TTTVTLAAGSYVYICHFPLHEQYGMVGVVTARS"
     gene            complement(1481..1723)
                     /locus_tag="VFV20_03285"
     CDS             complement(1481..1723)
                     /locus_tag="VFV20_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287401.1"
                     /translation="MADLIAHECVAPEHRPTLDHPDKLTIHRGAWAFCPFDAGARGHE
                     WRETGGATLDELRRRCGLGALPSGSSLSKARVKSSI"
     gene            complement(1821..>2855)
                     /locus_tag="VFV20_03290"
     CDS             complement(1821..>2855)
                     /locus_tag="VFV20_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEU5287402.1"
                     /translation="RSKLEQLVGDLVQATVEPVKRCLADAGVDAGKVDEIVLVGGMTR
                     MPLVVETVKKLFGKEPNKSVNPDEVVAVGAAIQAGVLKGEVKDILLLDVTPLSLGIET
                     LGGVATKLIERNSTIPTSKSQIFTTASDGQTQVEIHVVQGERELAADNKSLARFILDG
                     IPPSPRGIPQIEVSFDIDANGIVNVKAKDKATSKEQHVTITASSGLSKDEVDKLVKDA
                     QAHAEEDRRNVERIQTRNEADTFAYQIEKTLRDLGDKVPADTRTDVEGKIAALRETLK
                     GDDTARIKTAMDDLRNAAQKIGELMYQQQQQAGQQQPEGEPQPGEPAGATAGEKKSDD
                     TIEGEFKEQS"
BASE COUNT          356 a         1045 c          944 g          510 t
ORIGIN      
        1 gggggccggc caggacagga gggatcgaga tgccatcgat catccgtgtc cgacgacggg
       61 tcctcgccct cctcgccggc acgctcctgg tcgcgttcag cgcggccccc gctctagcct
      121 gcggcgggct catcggtccc aatggggccg tcaacctcct gcggacgacg acgttcgcgg
      181 gctaccacgc tggcgaggag cactacgtca ccgcgttcca gttcgcgggc ggcggcgggg
      241 ccttcggctc gatcacgccg ctgccgggca tcccgacgag cgtcgtgaag ggtggcgact
      301 ggacgctcca gcgcctcgtc cgcgagacga accccgtccc ggaggccgcg gtattcgcgt
      361 ctgactccgt gcgtgctggt gccgtcgagc tgatgaaggt caagatcgac gccctcgaca
      421 tcacggtcct caaaggcggc gccgccgaca tcgggacctg ggcatcggct cacggcttcc
      481 gccttccgcc ggacgcgccc gaggtcctcg acttctacgc ggcgcgcagc ccgatcttca
      541 tggccgccgt gttcgacgcc gacgcggcca aggcacgcgg ccagcgcatc ggcgacggca
      601 cgcccgtgca cctcgtgatc ccgacgccgt caccgtgggt gccgctgcgc atcctcggcc
      661 tcggcaagac ggcccaggac agggtggacg cggatgtcta cctcctcacc gacatcaagc
      721 cgcagctgct gccggcgccg ctcggtgtca acggcatccg catcgaccac agcgcgacgg
      781 cgaccgactc gctcctgaac gatctgcgct cggacaaggg gatgtcgtgg gtcccgtcct
      841 cgggatggct caccaagatc gtcgtggacg cgagcgccgg tcagctgcgc tatgacctcg
      901 cggtgagcac caccgcggcg gtgccgtcgc cagtcagcgc cggcctcgag gcgcccacgg
      961 ccccgctgcg ctcgcagacc acggagctca ccggcttcgt gatggccggc atcgtgctca
     1021 tcggcctcgg cggcctgctc atcctgcgca cgccggcgcc ggcacgcgta tgaccgctcg
     1081 gctcatcgtc ctcgccctcg ccgcatccgc cctctgggcg ggtgcggcct gtgggaccgc
     1141 gcaagcctcg acggtgcggc aggtcgagat ccgcatccac tattcgaagt tcgagcccgc
     1201 gaccgtgagc gtcccggcgg gcgttccggt cacgttcgtg atccgcaacc tggatccgat
     1261 cgaccatgag tggctcatcg gcgacgcggc gttccacgca cgccaccgct cggggaccga
     1321 gccggtccac ggcgaacgcg ccgacgaggt gtcgctccct gccggggcca cgcggacgac
     1381 gaccgtcacg ctcgcggccg gctcctacgt gtacatctgc cacttcccgc tgcacgagca
     1441 gtacgggatg gtgggcgtgg tgacggcgcg ctcgtaaggc ctagatcgac gacttcacgc
     1501 gtgctttcga gagactgctg ccgctcggaa gcgcgccgag gccgcatctg cgcctgagct
     1561 cgtcgagcgt ggcgcctccc gtctcgcgcc actcgtggcc cctggcgccc gcgtcgaacg
     1621 gacagaacgc ccacgcgccg cggtggatcg tcagcttgtc gggatgatcc aaggtcggcc
     1681 ggtgctctgg tgcgacgcat tcgtgcgcga tgagatccgc catcgaagtc cccttcccct
     1741 gtgcacgggt ccgggcccgt tcgccctctc gccggccccg cggaaccgcg tccgcggggc
     1801 cgtgtgccgt cctcgccagg ctagctctgt tccttgaact cgccctcgat cgtgtcgtcc
     1861 gacttcttct cgccggccgt cgcgccggcg ggctcgcccg gctgcggctc gccttccggc
     1921 tgctgctgtc cggcctgctg ttgctgctgg tacatgagct cgccgatctt ctgcgccgcg
     1981 ttacgcaggt cgtccatcgc ggtcttgata cgcgcggtgt cgtcgccctt gagcgtctcg
     2041 cgcagagcgg cgatcttccc ctccacgtcc gttctggtgt cggccggcac cttgtcgccg
     2101 aggtcgcgca gcgtcttctc gatctggtag gcgaacgtgt cggcctcgtt gcgggtctgg
     2161 atccgctcga cgttgcggcg gtcctcttcc gcgtgcgcct gcgcgtcctt cacgagcttg
     2221 tcgacctcgt ccttgctgag gccgctcgag gccgtgatgg tgacgtgctg ctccttgctc
     2281 gtcgccttgt ccttcgcctt cacgttcacg atgccgttcg cgtcgatgtc gaaggacacc
     2341 tcgatctgcg ggatgcctcg cggcgacggc gggatgccgt cgaggatgaa ccgcgcgagg
     2401 ctcttgttgt ccgcggcgag ctcccgctcg ccctggacga cgtggatctc gacctgggtc
     2461 tggccgtcgc tcgccgtcgt gaagatctgc gacttcgacg tcgggatcgt gctgttgcgc
     2521 tcgatgagct tggtggccac gccaccgagc gtctcgatgc cgagcgacag cggcgtgacg
     2581 tccagcagaa ggatgtcctt cacctcgccc ttcagcacgc cggcctggat cgcggcgccg
     2641 accgccacga cctcgtccgg gttcacgctc ttgttcggct ccttgccgaa gagcttcttg
     2701 acggtctcca cgacgagcgg catgcgcgtc atgccaccca cgaggacgat ctcgtcgacc
     2761 tttccggcgt cgacgccggc gtccgcgagg cagcgcttca cgggctcgac ggtcgcctgg
     2821 acgaggtcgc cgacgagctg ctcgagcttg ctgcg
//
LOCUS       DASTZR010000146         4000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_216907, whole genome shotgun sequence.
ACCESSION   DASTZR010000146 DASTZR010000000
VERSION     DASTZR010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216907"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1115)
                     /locus_tag="VFV20_03295"
     CDS             complement(213..1115)
                     /locus_tag="VFV20_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287403.1"
                     /translation="MEAPQLRPLGVGDIVDRVFALYRSRPLMFLAVAAVPYLLLFVVI
                     AGLGLAFATSLVALGGPLADIAAGRVPDLAAPSMRAAIVTAVALAIVSGVLAIVILSA
                     QSAGLIAATSSLYLRRPVGAGEAFREGLRAAPRIIGASILVFLIVVVLWIALGVVLVL
                     ANQAIVFALGILFGFVAMIYIFASTLVAPVVATLEHAGPMTALRRSWWLSDGSRWRII
                     GLQLLLLVLNAVIGALLSALFLGSFISDVTARTIVQQVANLVTNVAWAPVQWGTFAVL
                     YYDLRVRREAFDLQLAAEALPREP"
     gene            complement(1154..3439)
                     /locus_tag="VFV20_03300"
     CDS             complement(1154..3439)
                     /locus_tag="VFV20_03300"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEU5287404.1"
                     /translation="MFTAWGHIVYRVRWLVLAVSVLLLIASGFTAVQGGDLESGGFIE
                     TAESGRGTKLLNKELPSATNFTVIFSNGSLAWDDPRFRSAVEAAIAPLKGDPRVVSFV
                     TPYDPTVPDPTALHSRSGHDVSVTVNLKDELAIARDYYPELRGKITSDTLQVQATGVP
                     AINHGFNVILEKDLVRAETVSLPLAAILLLIVFGTVVAALIPLGVGILAVMGGVAGMF
                     LLSRTQTVSVYAENVVTLIGLGVAIDYSLFVANRFREELHRGRSVDDAIAIAIATSGR
                     AITFSGLTVAIGLSGMLFYEGTYLSSMGIAGAIVVASAVFYGLTFLPALLAVIGRRID
                     AIKIPILAGDPTGRGAWHAIAMAVMKRPIMVLLPLVALILLMGSPFLDLRLALGDVNM
                     LPKHEESRAAYEKLTAEFPGAGQNHVEVIVNYAGGQPLAADHIPQLVEFQKQLAAVPR
                     VEKVNSPVSLDPRLGAAEYTALYTVPAAQRPPQLQALVGAVGEARLAALLKQTVGEHI
                     VLFDVVTNERFGSDDARNTVKAFRAIAPPAGSETLVTGFSAIDLDTVSFIIGQTPIAV
                     GYIMIATMIVLFFLLGSVVLPLKAVIMNLLSISASFGALVWVFQEGHLSEQLGFVATG
                     SIDPTLPVIMFCTIFGLSMDYEVLLLSRIQEEYRRLGDNTAAVAEGLQRSGRLITGAA
                     AIMVIVFSAFGLAEILIIKAIGLGMALAVAIDATIVRTLVVPATMRLLGRVNWWAPGP
                     LRWLYRRLGLGEQATPAVETV"
     gene            complement(3582..3654)
                     /locus_tag="VFV20_03305"
     tRNA            complement(3582..3654)
                     /locus_tag="VFV20_03305"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3619..3621),aa:Pro,seq:tgg)
     gene            3708..>4000
                     /locus_tag="VFV20_03310"
     CDS             3708..>4000
                     /locus_tag="VFV20_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287405.1"
                     /translation="MIIVVEGPSGAGKTTWTRTHGGAHALLEDLPDNTAVPTDEEAAA
                     RFWVDRNVARWERVLDREQRDGLVVVDTDPFKLHYTWSLYQEGIASRRQWELER"
BASE COUNT          709 a         1325 c         1452 g          514 t
ORIGIN      
        1 cgacggcagc ccgagcgtgt cggtgcgtcg cagcagcgcc tggacgtccg cgatgagcgc
       61 gctccgcgcg gactgcggcg cgccacgcgc gcgcacgagc gtgtcgtgag cctgcgccag
      121 acgctgctgg tactgcgcga cggtgacggt gtccgcgagc gcggcgctcg gcgcgacgag
      181 cgtcgcgagc agcgcgagga cgaaggcgcg cgctagggct ctcgcggcag cgcctccgcg
      241 gcgagctgga gatcgaacgc ctcgcggcgg acgcgcaggt cgtagtagag caccgcgaag
      301 gtgccccact gcaccggcgc ccacgcgacg ttcgtcacga gattcgcgac ctgctggacg
      361 atcgtgcgcg ccgtcacgtc cgagatgaac gagccgagga acagcgccga caggagcgcg
      421 ccgatgaccg cgttcagcac gagcagcagc agctgcaggc cgatgatgcg ccagcggctg
      481 ccgtccgaga gccaccacga gcgccgcagg gcggtcatgg gcccggcgtg ctcgagcgtg
      541 gcgacgaccg gcgcgacgag ggtcgacgcg aagatgtaga tcatcgcgac gaagccgaag
      601 aggatcccga gcgcgaagac gatcgcctgg ttcgcgagca ccagaacgac gccgagcgcg
      661 atccagagca ccaccacgat gaggaacacg aggatgctcg cgccgatgat gcgcggcgcc
      721 gcgcggaggc cctcgcggaa ggcctcaccg gcgccgaccg gccggcgcag gtacagcgac
      781 gaggtcgccg cgatcagacc ggcgctctgc gccgacagga tcacgatcgc caggacccct
      841 gacacgatcg cgagggccac cgcggtgacg atggcggcac gcatgctcgg ggccgcgagg
      901 tccgggacgc gcccggcggc gatgtcggcg agcggcccgc ccagcgcgac gaggctcgtc
      961 gcgaaggcga gcccgagccc ggcgatgacg acgaagagca gcaggtaggg caccgcggcg
     1021 acggccagga acatgagcgg acgcgagcgg tacagggcga acacgcgatc gacgatgtcg
     1081 ccgacaccga gcggacggag ctgcggcgct tccacgtcgc gaatcgtagt gccgagcccg
     1141 ccgccgctcg tcgctacacc gtctccacgg cgggtgtcgc ctgttcaccg aggccgaggc
     1201 ggcggtacag ccaccggagc gggccgggcg cccaccagtt cacgcggccc aacagccgca
     1261 tcgtcgcggg caccacgagc gtgcgcacga tggtggcgtc gatcgcgacg gcgagcgcca
     1321 ttccgaggcc gatcgctttg atgatgagga tctccgcgag cccgaacgcc gagaagacga
     1381 tcaccatgat cgccgcggcg ccggtgatga gacggccgct ccgctgcagg ccctccgcga
     1441 cggcggcggt gttgtcgccg agacgccggt attcctcctg gatgcgcgac agcagcaaca
     1501 cctcgtagtc catggagagg ccgaagatcg tgcagaacat gatcaccggc agcgtcgggt
     1561 cgatcgagcc cgtggcgacg aagccgagct gctcggagag gtgcccttcc tggaagaccc
     1621 agaccagcgc accgaacgag gcgctgatcg agagcaggtt catgatgacg gccttgagcg
     1681 gaagcaccac cgagccgagg aggaagaaca gcacgatcat cgtcgcgatc atgatgtagc
     1741 cgaccgcgat gggcgtctga ccgatgatga acgacaccgt gtcgagatcg atcgcgctga
     1801 agccggtgac gagcgtctcg gatcccgcgg gaggcgcgat cgcgcggaac gccttcaccg
     1861 tgttgcgcgc gtcgtcgctg ccgaaccgct cgttcgtcac gacatcgaag aggacgatgt
     1921 gctcgcccac ggtctgcttc agcagcgccg cgagtcgcgc ctcgccgacg gcgccgacga
     1981 gcgcctgcag ctgcggcggg cgctgggccg cggggaccgt gtagagcgcg gtgtactcgg
     2041 cggcgccgag gcgcgggtcg agagagaccg gcgagttcac cttctccacg cgcggcaccg
     2101 cggcgagctg cttctggaac tcgacgagct gcgggatgtg atccgccgcg agcggctggc
     2161 cgcccgcgta gttcacgatg acctcgacgt ggttctggcc cgcaccgggg aactccgcgg
     2221 tgagcttctc gtacgcggcg cgcgactcct cgtgcttcgg gagcatgttc acgtcgccga
     2281 gggcgagccg caggtcgagg aacggcgagc ccatgagcag gatgagcgcg acgagcggca
     2341 gcaggaccat gatcgggcgc ttcatcacgg ccatggcgat cgcgtgccac gcgccgcgac
     2401 cggtcgggtc gcccgcgagg atcgggatct tgatcgcatc gatgcggcgg ccgatcacgg
     2461 cgagcagcgc cggaaggaac gtgagaccgt agaacaccgc gctcgcgacg acgatcgcgc
     2521 cggcgatgcc catagacgag agatacgtcc cctcgtagaa gagcatgccg gagagtccga
     2581 tcgcgacggt gaggccggag aacgtgatcg cgcggcccga cgtcgcgatg gcgatggcga
     2641 tcgcgtcgtc cacactcctg ccgcggtgca gctcctcgcg gaagcggttc gcgacgaaga
     2701 gcgagtaatc gatcgcgacg ccgagaccga tgagcgtcac gacgttctcg gcgtacaccg
     2761 agaccgtctg cgtgcgcgag agcaggaaca tcccggccac gccgcccatc accgcgagga
     2821 tgccgacgcc gagcgggatg agcgccgcga cgacggtgcc gaacacgatg agcaggagga
     2881 tcgccgcgag cgggagcgac accgtctcgg cgcgcacgag gtccttctcg aggatcacgt
     2941 tgaagccgtg gttgatggcg gggacgcccg tcgcctggac ctgcagcgtg tcggaggtga
     3001 tcttgccgcg cagctccggg tagtagtccc gcgcgatggc gagctcgtcc ttgaggttca
     3061 cggtcacgga cacgtcgtgc ccgctccgcg agtggagcgc ggtcgggtcc gggaccgtcg
     3121 ggtcataggg cgtgacgaac gagacgacgc gcgggtcgcc cttgagcggc gcgatcgccg
     3181 cctcgaccgc cgaacggaac ctcgggtcgt cccacgcgag ggagccgttc gagaagatga
     3241 cggtgaagtt cgtggcgctc gggagctcct tgttcaggag cttggtgccg cggccggatt
     3301 cagcggtctc gatgaagccg ccgctctcga ggtcgccgcc ctgcaccgcg gtgaagccgg
     3361 aggcgatgag gaggagcact gagacggcca gcacgagcca gcggacgcga tagacgatgt
     3421 gaccccacgc cgtgaacacg cggacctcct gcctcaactc gcgatgcggt gatgactagg
     3481 ttacgggtgc agacggaaat gcgcgccggt acggagtgac aattcgtgtc cgtattcgcc
     3541 ggacgccgct acgtgcaggg tgcctccgca gcttggtgct gtcggggtgg cccgactcga
     3601 acgggcggcc tgagcgtccc aaacgctccg cgctaccaac tgcgccacac cccgcgagtc
     3661 gaacaatcat cccaccaacg ggtcttcagg gctagcatcg cgaactcgtg atcatcgtcg
     3721 tcgagggtcc gagcggtgcc ggcaagacga cttggacgcg cacgcacggc ggcgcgcacg
     3781 cgctcctgga ggatctcccc gacaacacgg cggtgccgac cgatgaggag gccgccgcgc
     3841 gcttctgggt cgaccgcaac gtcgcgcggt gggaacgcgt gctcgaccgg gagcaacgcg
     3901 acggcctcgt cgtcgtcgac accgatccgt tcaagctcca ctacacgtgg tcgctctacc
     3961 aggagggcat cgcatcacga cggcagtggg agctcgagcg
//
LOCUS       DASTZR010000147         2406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_609274, whole genome shotgun sequence.
ACCESSION   DASTZR010000147 DASTZR010000000
VERSION     DASTZR010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609274"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VFV20_03315"
     CDS             <1..298
                     /locus_tag="VFV20_03315"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008496050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEU5287406.1"
                     /translation="LLAGRSAAALSEKIHRAGAVFIGGRSAVAFGDYVAGTNHCLPTG
                     GTARWSSALRVEDFVRWTSRVELRGDLHRLAKAGATVARHERMRYHAESMEARG"
     gene            298..942
                     /gene="hisB"
                     /locus_tag="VFV20_03320"
     CDS             298..942
                     /gene="hisB"
                     /locus_tag="VFV20_03320"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017718436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEU5287407.1"
                     /translation="MPARRPAPRREPRRTDREPARRATRERNTKETRIQVRFDLDGAG
                     KHDVSTGLPFLDHMLELVAVHGLFDLVVKAKGDIDVDQHHTVEDLGLVLGAAIDDALG
                     DRKGLVRYASLEIPMDECLVSVALDLGGRPFFASDLKPKTRIVGSFDSTLVPDFFVAL
                     TQGAKCTLHIRQLSGGNTHHLLEAAFKALGRALDQASQRDPRRRGVPSSKGRIG"
     gene            960..1457
                     /locus_tag="VFV20_03325"
     CDS             960..1457
                     /locus_tag="VFV20_03325"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5287408.1"
                     /translation="MVFSGLPGSGKTAIARPLARTLGLTLLELDLIERPLLRRFAGDA
                     LGWAGYEILTVIADDNLALGTGVVLDCVTWTNAVREQWRTLAGRHRARFRPIEVVCAD
                     RELLRARVEGRDGGGEVRKNAWPHVEASFARYEPWATDRLALDATRPLDELVRAAVEY
                     VKGGS"
     gene            1454..2062
                     /locus_tag="VFV20_03330"
     CDS             1454..2062
                     /locus_tag="VFV20_03330"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5287409.1"
                     /translation="MIAGIDHVVLVTLDLASTVAEWRERGFAVHMGGAHAGGLSENAL
                     VGLADGSYVELFAFVDLARARTAHPWAPVAERGGGWADFALASDDLARDAAALGDLVA
                     RPPEDGGRTRPDGLRVAWRSARLVSPLPFLIQDVTARELRVPSGEAARHANGTSGIAS
                     VIVGARDPHAVTRRYAMLRDIGAPPIEIVAAERDGLREVTFR"
     gene            2126..>2406
                     /locus_tag="VFV20_03335"
     CDS             2126..>2406
                     /locus_tag="VFV20_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEU5287410.1"
                     /translation="MISIVDYGVGNLANVERALRRAGATAVQITDDAAAILGSRAVVL
                     PGVGHFGHCARELASRGLDGPMHEVRERRVPLLGLCVGMQLFYESSEEDA"
BASE COUNT          318 a          876 c          875 g          337 t
ORIGIN      
        1 cctgctcgcg ggccggtcgg ccgcggcgct ctcggagaag attcaccgcg cgggcgccgt
       61 cttcatcggc ggacgctcgg cggtcgcctt cggcgattac gtcgccggca cgaaccactg
      121 cctgcccacc ggcgggaccg cgcgctggtc gtcggcgctg cgggtggagg acttcgtacg
      181 ctggaccagt cgcgtggagc tgcgcggcga cctgcaccgg ctggcgaagg ccggcgcgac
      241 ggtcgcgcga cacgagcgga tgcgctatca cgcggagtcg atggaggcga gaggctgatg
      301 ccagcacgac gaccggcccc gcggcgcgag ccgcgacgaa cggaccgcga gcccgcgcgc
      361 cgcgcgacgc gcgagcgcaa caccaaggag acgcgcatcc aggtgcgctt cgatctcgac
      421 ggcgcgggaa agcacgacgt ctcgaccgga ctgccgttcc tcgatcacat gctcgagctc
      481 gtcgcggtgc acgggctctt cgacctcgtc gtgaaggcga agggcgacat cgacgtggac
      541 cagcatcaca ccgtggagga cctcggcctc gtgctcgggg cggcgatcga cgacgcgctg
      601 ggcgaccgaa agggcctggt gcgttacgcg tcgctggaga tcccgatgga cgaatgcctc
      661 gtgtcggtcg cgctcgacct cggcggccgg ccgttcttcg cgagcgatct caagccgaag
      721 acgcgcatcg tcgggtcatt cgattccacg ctggtgcccg acttcttcgt cgcgctcacg
      781 cagggcgcga agtgcacgct ccacatccgc cagctctcgg gtggcaacac gcaccacctg
      841 ctcgaggccg cgttcaaggc gctcggccgc gcgctcgatc aggcatcgca gcgcgacccg
      901 cggcgccgcg gcgtgcccag ctcgaagggg cgcatcggct gacgctgcca ctcaccctgg
      961 tggtcttctc gggcctgccg ggaagcggca agaccgccat cgcacgcccg ctcgcgcgca
     1021 cgctcggcct cacgctcctc gagctcgacc tcatcgagcg gccgctgctc cgtcgcttcg
     1081 cgggcgacgc gctgggctgg gcgggctacg agatcctcac ggtgatcgcc gacgacaacc
     1141 tcgcgctcgg gacgggcgtc gtgctcgact gcgtcacctg gacgaacgcc gtccgcgaac
     1201 agtggcgcac gctcgccggg cgtcatcggg cgcgcttccg gccgatcgag gtcgtctgtg
     1261 ccgaccgcga actgcttcgc gcgcgcgtcg agggacgcga tggcggtggc gaggtgcgga
     1321 agaatgcctg gccgcacgtc gaggcgtcgt tcgcgcgcta cgagccgtgg gcgacggacc
     1381 ggctcgcgct cgacgccacg cgaccgctcg acgagctggt gcgcgcggcg gtcgagtacg
     1441 tgaagggcgg atcgtgatcg ccggcatcga ccacgtcgtc ctcgtcacgc tcgacctcgc
     1501 gagcaccgtg gccgagtggc gcgagcgggg cttcgcggtg cacatgggcg gcgcgcacgc
     1561 cggcggcctc tccgagaacg cgctcgtcgg gctcgccgac ggcagctacg tcgagctgtt
     1621 cgcgttcgtc gatctcgcgc gcgcccggac ggcacacccc tgggcgccgg tcgcggagcg
     1681 cggcgggggc tgggccgact tcgcgctcgc atccgacgac ctggctcgcg acgccgccgc
     1741 gctcggcgac ctcgtcgcgc gaccgccgga ggacggcggc cgcacccggc cggacggcct
     1801 gcgcgtcgcg tggcgttccg cgcgcctcgt ctcgccactt ccctttctca tccaggacgt
     1861 gaccgcgcgc gagctgcgcg tgccgagcgg cgaggcggcg cggcacgcga acggcacgag
     1921 cggcatcgcg agcgtgatcg tcggagctcg cgatccgcac gccgtgacgc gccgctacgc
     1981 gatgcttcga gacatcggcg cgccgccgat cgagatcgtg gcagcggagc gtgacgggct
     2041 ccgcgaggtg accttccgct agcgccgtga atcgtcgagc gcgccgcaag gcgcgcgcga
     2101 tcgtcttctg ccgtagcctc gacaggtgat ctccatcgtc gactacggcg tcggcaatct
     2161 cgcgaacgtc gagcgggcgc tccggcgcgc cggcgcgacc gccgtccaga tcaccgacga
     2221 cgcggcggcc atcctcggct cgcgcgccgt ggtgctgccg ggcgtcggcc acttcggcca
     2281 ctgcgcgcgc gagctcgcgt cgcgcgggct cgacggtccg atgcacgagg tccgggagcg
     2341 tcgcgtcccg ctcctcggcc tctgcgtcgg gatgcagctc ttctacgaat cgagcgagga
     2401 ggacgc
//
LOCUS       DASTZR010000148         2090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_739896, whole genome shotgun sequence.
ACCESSION   DASTZR010000148 DASTZR010000000
VERSION     DASTZR010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739896"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2090)
                     /locus_tag="VFV20_03340"
     CDS             complement(<1..>2090)
                     /locus_tag="VFV20_03340"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5287411.1"
                     /translation="RAAAPTGLERHRLSVAFQHLFRAFARETPVLVVLEDLHWADETS
                     LELLQHLARELRDARVLLLATYRSDEMHRRHPFLRTLADLQRERLVTEIQLKRLTLEE
                     TKELIRATMATRDPSVRVSDEFRDALYSRTDGNPFFTEELLKALVDSGGVYLQEGGWA
                     RKPIDELAIPGSIREAVRARVEELTPEARGTLSVAAVIGHRFGYDTLRAVTGLDEPTL
                     ERHLREFIEAQLAGEGDGTSEEYAFRHALTREVVYDDLLVRERKRSHRAVADALEVDT
                     RVEPSLVAHHLLAAGETARAVPKLLEAAARAMRASAPREAVTHYERAVDIGVQDAEVA
                     GVLEQLAEAYHLFDETLSLKAANEAAAVYRERGDHRGVSRMLRLASRNHWLKGDGEPA
                     QRLGREAIEQVAADDSIELARALAHMAGLRMTAGTRDDAIEYADRAIALAERFDDTFA
                     LANALVSKGSVIARTDPAAGLEIIDRGRQIAMRSGLAITASRGWNNMSLWLPFVGRSH
                     DEQLGFVREGIAWAQAHGVEAGSLSFLRNMQAALLSGRGDFTSAIADYEWIISNGGSL
                     GWAAKTFLVLAREGPTKVRDVVPELLRRAERAGESQGWVPLTGTAALILYADGRRPEA
                     DELMRRYLTRVRSDEGMHVIATGPWHSLTLGLALLWDNAELIDMVADQREGPTPKMVE
                     FRDRGVAAARAARR"
BASE COUNT          274 a          735 c          770 g          311 t
ORIGIN      
        1 cgcggcgggc tgcgcgggcc gcggccaccc cgcgatcgcg gaactcgacc atcttgggtg
       61 tcgggccttc gcgctggtcc gcgaccatgt cgatgagctc ggcgttgtcc cagagcagcg
      121 cgaggccgag cgtgagcgaa tgccacggac cggtcgcgat gacatgcatg ccctcgtcgg
      181 agcgcacgcg ggtgagatag cgcctcatga gctcgtccgc ctcgggacgc cgtccgtcgg
      241 cgtacaggat gagcgcggcc gtgccggtga gcggcaccca gccctgcgac tcgccggcgc
      301 gctcagcgcg gcggagcagc tcagggacga cgtcgcgcac tttcgttggg ccctcgcgcg
      361 cgaggacgag gaaggtcttc gctgcccatc cgagcgatcc gccgttcgag atgatccact
      421 cgtagtcggc gatcgcgctg gtgaagtcgc cgcggcccga gagcagggcg gcctgcatgt
      481 tgcgcaggaa gctgaggctt ccggcctcga cgccgtgcgc ctgcgcccag gcgatgccct
      541 cgcgcacgaa tcccagctgc tcgtcgtgcg accggccgac gaacgggagc cagagcgaca
      601 tgttgttcca gccccgcgat gccgtgatcg cgaggcccga ccgcatcgcg atctggcgtc
      661 cccggtcgat gatctcgagg cccgcggcag gatccgttcg cgcgatgacg ctccccttcg
      721 agacgagcgc gttcgcgagc gcgaacgtgt cgtcgaagcg ctcggcgagc gcgatcgcgc
      781 ggtcggcgta ctcgatcgcg tcgtcgcgcg tgcccgcggt catgcggagg ccggccatgt
      841 gcgcgagcgc acgcgcgagc tcgatcgagt cgtcggcggc gacctgctcg atggcctcgc
      901 gcccgagccg ttgcgcgggc tcgccgtcgc ccttcagcca gtggttgcgg ctcgcgaggc
      961 gcagcatgcg cgagaccccg cggtggtcgc cgcgctcccg gtagaccgcc gccgcctcgt
     1021 tcgcggcctt gagcgagagc gtctcgtcga acaggtggta cgcctccgcc agctgctcga
     1081 ggacgccggc gacctccgcg tcttgcacgc cgatgtccac cgcgcgctcg tagtgcgtga
     1141 ccgcctcgcg cggcgccgat gcgcgcatcg cgcgcgccgc ggcctcgagc agcttcggca
     1201 cggcgcgcgc ggtctcgccg gccgccagca gatggtgcgc gacgagcgac ggctcgacac
     1261 gggtgtcgac ctcgagggcg tcggccacgg cgcggtggga ccgcttgcgt tcgcgcacga
     1321 ggagatcgtc gtagacgacc tcgcgtgtga gggcgtggcg gaaggcgtac tcctcgctcg
     1381 tgccgtcgcc ctcgccggcg agctgcgcct cgatgaactc acgtaggtgc cgctcgaggg
     1441 tcggctcgtc caggcctgtc accgcccgga gcgtgtcgta cccgaagcgg tggccgatga
     1501 cggcggcgac cgagagcgtc ccgcgcgcct ccggcgtgag ctcctcgacc cgcgcgcgta
     1561 cggcctcgcg gatggagccg gggatggcga gctcgtcgat cggctttcgt gcccagccgc
     1621 cctcctgcag gtagaccccg ccggagtcca cgagcgcctt gaggagctct tcggtgaaga
     1681 aggggttgcc gtcggtgcgc gagtagagcg cgtcgcggaa ctcgtcgctc acgcgcacgc
     1741 tcggatcgcg cgtcgccatc gtcgcccgga tgagctcctt cgtctcctca agggtgagcc
     1801 gcttcagctg gatctcggtg acgagccgtt cgcgctgcag gtccgcgagg gtgcgcagga
     1861 acggatggcg gcgatgcatc tcgtcgctgc ggtacgtggc gagcagcagc acacgagcgt
     1921 cgcgcagctc gcgcgcaagg tgctgcagca gctcgaggct cgtctcgtcg gcccagtgga
     1981 ggtcctcgag cacgacgagc actggcgttt cccgcgcgaa cgcgcggaac aggtgctgga
     2041 acgcgaccga gagccggtgg cgctcgagac cggtcggcgc cgcggcgcgc
//
LOCUS       DASTZR010000149         3169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1105482, whole genome shotgun sequence.
ACCESSION   DASTZR010000149 DASTZR010000000
VERSION     DASTZR010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105482"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VFV20_03345"
     CDS             <1..475
                     /locus_tag="VFV20_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU5287412.1"
                     /translation="VTTLVQVNVNGTLLDAAIDARWTLSEFLRDQLGLTGTKEGCNRG
                     ECGSCTVIVDGRAILGCMTLAVEVAGKTVQTVESLAGGGKMNALQQGFWDAGATQCGF
                     CIPGMLMSATALLAAKPKPTEDDVRLALSGNLCRCTGYRKIVEGVLVGSGQAPKA"
     gene            487..2889
                     /locus_tag="VFV20_03350"
     CDS             487..2889
                     /locus_tag="VFV20_03350"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEU5287413.1"
                     /translation="MSKTLSTQEIIALLDDTNFLWQVAEWPDDHIDEVTQINEYLRSV
                     TPQAPAWIRAASQQPLKIVGTRQRRYEDPARLMGQALYSSDIKLPGMLYTAILPSPHA
                     HALIEDIDTSAAGALPGVRAILTYKNAPKTVIGGPPDQFLMNQEVHFAGEEVAWVAAD
                     DIQTAREATRLIKVQYKVLPSISDVEKALAAGAPDIVGTKSGNKVPGSTAPVKRGNFD
                     TAYASAATKHEGRYTTETLQHMYLEPRASVARWEGPDRLVVWTGTQYVAGVRNDLAGY
                     FNMPRSHVRVICENVGGGFGAKSPAGRQARAAAVLAQMTAKPVRVVYDRPTDMRSAIH
                     RFADIVELKAGVDKDGKLVAYKAYNIGDAGAYNAGTSALVPITRLYRVPNALFEFQGG
                     ITNRGPSGAQRCVGDPQGTWCQEIFMDELAEKAGIDPVEFRLRNFETERFQTNNLPWA
                     SCGIVECVQRAAKEIGWTTKWHKPAAKITGRKAHGIGMAAHSCAHGSMSVPMTAMMRL
                     DRDGSLDVNETSTEHGGGEATVMTMIAAETLGIPLSMCQPHWGDAGANPDSGSSGGSK
                     QTIGTGNAMRDAALDLKAQLLTIATSGAKPMLAAKPEDCDTADGYVFIKTDPSKKVAF
                     KDVVASTGNPLLGTGAHTVPPGWTQGTFSAGFAEVEVDLDTGELTLLRYVLSNDVGKA
                     VNVLAVEQQMEGGASMAIGMGIGEEIKHDPQANFPVHWNWENYAMPTTLEHPKWADFK
                     PIIVEPIDALGPFGAKGVGEPPTAPPPPAIANALYNAIGVRIHDAPITRDKILAAIAK
                     MK"
     gene            2903..>3169
                     /locus_tag="VFV20_03355"
     CDS             2903..>3169
                     /locus_tag="VFV20_03355"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEU5287414.1"
                     /translation="MKRFEHVNATSVDEAVKLLSAQNARAIAGGTDLLGVLKDQLLPT
                     ERVVNLKTIPGLDQIKEEGGELRIGALARLADIAASALVAQKAPA"
BASE COUNT          572 a         1097 c         1091 g          409 t
ORIGIN      
        1 ggtgacgacg ctcgtgcagg tcaacgtgaa cgggacgctg ctcgacgcgg cgatcgacgc
       61 gcgctggacg ctctccgaat tcctccgcga tcagctcggc ctcaccggca cgaaggaggg
      121 ctgcaaccgc ggcgagtgcg gctcgtgcac cgtcatcgtc gacggtaggg ccatcctcgg
      181 ctgcatgacc ctcgcggtcg aggtcgcggg caagacggtg cagaccgtcg agagcctcgc
      241 gggcggcggc aagatgaatg ccctccagca gggcttctgg gacgcgggcg cgacccagtg
      301 cgggttctgc atcccgggga tgctcatgag cgcgaccgcc ctgctcgccg cgaagccgaa
      361 gccgaccgag gacgacgtgc ggctcgcgct ctcgggcaac ctctgccgct gcaccggcta
      421 tcgaaagatc gtcgagggcg ttctggtcgg gtccggccag gcgccgaagg cataggggag
      481 gtcgtgatga gcaagacgct ttcgacgcaa gagatcatcg ccctcctcga cgacacgaac
      541 ttcctctggc aggtcgcgga atggcccgac gaccacatcg acgaggtcac gcagatcaac
      601 gagtacctcc gatccgtcac accgcaggcg cccgcgtgga tccgtgccgc gtcgcagcag
      661 ccgctcaaga tcgtcggcac tcggcagcgc cgctacgagg atcccgcgcg gctcatgggc
      721 caggcgctct actcgtcgga catcaagctg ccgggcatgc tctacacggc gatcctgccg
      781 tcgccacatg cccacgcgct catcgaggac atcgacacgt cggcggccgg ggcgctgccc
      841 ggcgtgcgcg cgatcctgac gtacaagaac gcgccgaaga cggtcatcgg cggtccgccg
      901 gaccagttcc tcatgaacca ggaggtgcac ttcgcgggcg aggaggtcgc gtgggtcgcg
      961 gccgacgaca tccagaccgc gcgcgaggcg acgcgcctca tcaaggtcca gtacaaggtg
     1021 cttccgtcga tcagcgacgt cgagaaggcg ctcgccgccg gtgccccgga catcgtcggg
     1081 acgaagagtg gcaacaaggt ccccgggagc acggcaccgg tcaagcgcgg caacttcgac
     1141 accgcgtacg cctccgccgc caccaagcac gagggccgct acaccacgga gacgctccag
     1201 cacatgtacc tcgagccacg cgcgtcggtc gcccgatggg agggccccga tcgcctcgtc
     1261 gtgtggacgg ggacgcagta cgtggccgga gtgcgcaacg acctcgccgg ctacttcaac
     1321 atgccgcgca gccacgtccg cgtcatctgc gagaacgtcg gcggtggctt cggggcgaag
     1381 agccccgccg gtcgccaggc gcgcgccgcc gcggtgctgg cgcagatgac ggcgaagccg
     1441 gtgcgcgtgg tgtacgaccg tccgaccgac atgcggtcgg cgatccaccg gttcgctgac
     1501 atcgtcgagc tgaaggcggg cgtcgacaag gacggcaagc tcgtcgcgta caaggcctac
     1561 aacatcggcg acgccggcgc gtacaacgcg ggcacgagcg cgctcgtccc gatcacgcgc
     1621 ctgtaccgcg tcccgaacgc gctcttcgag ttccagggcg ggatcaccaa ccgcggtccg
     1681 tccggcgcgc agcgctgcgt cggcgatccg cagggcacct ggtgccagga gatcttcatg
     1741 gacgagctcg ccgagaaggc gggcatcgac cccgtggagt tccgtctgcg gaacttcgag
     1801 accgagcggt tccagaccaa caacctgccg tgggcatcct gcggcatcgt cgagtgcgtc
     1861 cagcgcgccg cgaaggagat cggctggacg acgaagtggc acaagccggc ggcgaagatc
     1921 accggccgca aggcgcacgg catcggcatg gccgcgcact cgtgcgcgca cggctcgatg
     1981 agcgtgccga tgaccgcgat gatgcggctc gaccgcgacg gcagcctcga cgtcaacgag
     2041 acatccaccg agcacggcgg cggcgaggcg acagtgatga cgatgatcgc cgccgagacg
     2101 ctcggcatcc cgctgtccat gtgccagcct cactggggcg acgccggcgc gaacccggac
     2161 tccgggtcgt ccggcggcag caagcagacc atcgggaccg gcaacgcgat gcgcgacgcg
     2221 gcgctcgacc tgaaggcgca gctcctcacc atcgcgacga gcggcgccaa gcccatgctc
     2281 gccgcgaagc ccgaggactg cgacacggcc gacgggtacg tgttcatcaa gaccgacccg
     2341 agcaagaagg tggcgttcaa ggacgtggtc gccagcaccg ggaacccgct gctcgggacc
     2401 ggagcgcaca ccgtgccgcc cggctggacg cagggcacct tctccgcggg cttcgcggag
     2461 gtcgaggtcg acctcgacac cggcgagctc accctgctgc ggtacgtgct cagcaacgac
     2521 gtcggcaagg ccgtgaacgt cctggccgtc gagcagcaga tggagggcgg cgcgtcgatg
     2581 gcgatcggca tgggcatcgg agaggagatc aagcacgacc cgcaggcgaa cttcccggtg
     2641 cattggaact gggagaacta cgcgatgccc acgacgctcg agcacccgaa gtgggcggac
     2701 ttcaagccga tcatcgtcga gcccatcgac gccctcgggc cgttcggcgc caagggcgtg
     2761 ggcgagccgc cgacggcgcc gccgccgccc gcgatcgcga acgcgctgta caacgccatc
     2821 ggcgtacgca tccacgacgc gcccatcacg cgcgacaaga tcctcgcggc gatcgcgaag
     2881 atgaagtgag aggagaacgt ctgtgaagcg attcgaacac gtgaacgcga cctctgtcga
     2941 cgaggcggtg aagctcctct cggcacagaa cgctcgcgcc atcgccggcg gcaccgacct
     3001 tctcggtgtg ctgaaggacc agctccttcc caccgagcgg gtggtgaacc tcaagaccat
     3061 cccgggcctg gaccagatca aggaggaggg cggggagctg cgcatcggcg cgctggccag
     3121 gctcgccgac atcgccgcct cggcgctggt ggcccagaag gcccccgcg
//
LOCUS       DASTZR010000150         7338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_972101, whole genome shotgun sequence.
ACCESSION   DASTZR010000150 DASTZR010000000
VERSION     DASTZR010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7338
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972101"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..1461
                     /gene="dnaB"
                     /locus_tag="VFV20_03360"
     CDS             139..1461
                     /gene="dnaB"
                     /locus_tag="VFV20_03360"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU5287415.1"
                     /translation="MTVETRVDRQVPFDERAEQHVLGSLLIDRDAIFKIADLLATSDF
                     YVARHQRIYASIYELLQRRERIDPLTVQVELARREELDRVGGAAYLRTLTDLVANAVE
                     IERHARVVRDRAILRRLLNAATQIAADAYTEPTDIPLTLDRAEQRIFALRDDTTNAQL
                     RHIQTALQDNFQHLTERMDRPYEVSGIPSGFRELDFFTEGFTQGDLVVLAARPSVGKT
                     SLALAMAFNIAKRGHPTLVFSLEMDAKQIVSRFLAMNSRTDLLALRTGNVFDTEAASA
                     LGGVPILIDDTPGLSIMELRTKARRASTNDRLEVIIVDYIQLMRTGGDEENRVQEIAT
                     ITRNLKSLARELNVVIIGLSQLSRAAGDSGLEPKLSTLRESGAIEQDADIVLMLWKDK
                     EDTPPGAPRLIHGSVAKNRNGPTGIFQLLFAGEQARFFSKAADDQMPI"
     gene            1458..2792
                     /gene="dnaB"
                     /locus_tag="VFV20_03365"
     CDS             1458..2792
                     /gene="dnaB"
                     /locus_tag="VFV20_03365"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU5287416.1"
                     /translation="MTIDVERSAPKPPPEKPEAEASLIGSILIDAHIYEETVRGRIAP
                     AELSRESHRLVLAAIERVWERGEPLGFQSVQSDLAARGELDAVGGAQFLVGLMQNVPT
                     VAHAETYATIVERTALLRRLIGAANEIAHLALTREDARSAVEDAQELLFAVSESNLHR
                     DVVPLELALRRFADQITARADDHHEGVPTGFASLDAKTGGLQPNDLILLAGRPGLGKT
                     SFALNIVWNAAAVERKTCAVFSLEMSEMQVVQRLISMTSEIDGNRMRRGRLSPEEFRS
                     ISAASDQLQKAPIFVEESSQLTVTDILAKARRLQAERGLDLLVIDYLQLIEGAGDDEG
                     RVLEVARISRGLKAIARELQVPVIALSQLSRQIETRGTEPMLSDLRESGALEADADLV
                     MFLWQKDRKDRDENVVRLKLAKHRNGPTGDFDLHFQSELTRFRDLGETRVEA"
     gene            complement(2789..4321)
                     /locus_tag="VFV20_03370"
     CDS             complement(2789..4321)
                     /locus_tag="VFV20_03370"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEU5287417.1"
                     /translation="MTAAARSDLDLLMRSAEELLLAASDEKRLLSLGTELLGEQYGYG
                     ARYILLRDETRDDLYTAAFAGPLAEIEAAGRFRTEPGRGVSGACFATASVVNVPDVSM
                     DPRFIRVLPSCRSEICIPIVAGDRALGVLGIESDHTAAFSAHDERVLSAYARLLAMAL
                     MHARQHQARQKDIAELQAVSEVAQRAAALDLAGTLDAVCGSLRSITTSDSVAMHLWDS
                     RAETLRMAALSYDAAVYASDYESSLRAMRLELGEGMVGWAALHREPLRVDDVGADARA
                     LAVPGTPRHSKSAIVIPLLVEDRLVGVARLVKMGVSSYTDDHFRFARTIASQAALAIA
                     AAEAHEEIRRLSVTDELTGAYNTRHAMQRLREEIESARRHDEHVSLLVIDGDCMKQVN
                     DQYGHAEGNRLLIELTAAMSASLRLTDVLGRFGGDEFIAILPRTTAKDALVTAERLRT
                     SVAARECRTSWGEPIRATVSVGIATYPQHGRTADDLFREADRRLYEAKQAGRNRVTTA
                     AG"
     gene            complement(4460..4909)
                     /gene="rplI"
                     /locus_tag="VFV20_03375"
     CDS             complement(4460..4909)
                     /gene="rplI"
                     /locus_tag="VFV20_03375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU5287418.1"
                     /translation="MRVILKREVRGLGRPGDVKDVADGYAQNFLLPRGLAIEATAGEL
                     KHLAQERDAEKAKKDRAHADAEALAARLAGLRLVFHLKAGEQGKTFGSVTNKDVADAL
                     KREHKIEIDRTKIVLGEPLKTIGPHEVEIRILPDVRAKVAVAVEPAR"
     gene            complement(4920..5105)
                     /locus_tag="VFV20_03380"
     CDS             complement(4920..5105)
                     /locus_tag="VFV20_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287419.1"
                     /translation="MSREESGGGFFGAIEALLAIVGALTVMGVIAVAASVLAWEMELR
                     KSEQNGQLAEPTTPSTT"
     gene            complement(5212..5634)
                     /locus_tag="VFV20_03385"
     CDS             complement(5212..5634)
                     /locus_tag="VFV20_03385"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HEU5287420.1"
                     /translation="MPRGIQRPRNMNEALDTVRKLPTYGRLVWDLARDPRVPRQQKLV
                     LGALAAYLAFPIDIIPDFIPVIGQMDDVAVVILALDWFIKNAPPEVVEEHMSRIAARD
                     DTLAKDLDAAGSLLAGRASELRATLERMRSKGDQGGDA"
     gene            complement(5627..6382)
                     /locus_tag="VFV20_03390"
     CDS             complement(5627..6382)
                     /locus_tag="VFV20_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEU5287421.1"
                     /translation="MPVDSALILDYGMRTVWALLVVVAALLLARGVRRATMRMLARNR
                     AQANVTILLGNLAQVLVILVGVLGVLAIYTRDAFGWILTSFSVLGIVIGLSLQDILKN
                     FFAGIWVLVERPFRIGDTIEVGGQVGVVEEIAFRTTMLKTADNREVIIPNGVFMTSAV
                     VNLTRFPTRRGTLWLIVDDRERPIGADEIRSALGEAAGLVAEPKPAVELRSVSDGKAH
                     YLVSFWSADRDAAASAAIAALRARFPQGEVHGA"
     gene            6439..7047
                     /gene="lexA"
                     /locus_tag="VFV20_03395"
     CDS             6439..7047
                     /gene="lexA"
                     /locus_tag="VFV20_03395"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEU5287422.1"
                     /translation="MTKQDQQRAERILDFIRERIAEQGYPPTVREIAEAVGLASTSAV
                     HHHLMKLEREGKLQKDATRSRALTIPGSLGGRVVQAPIVGEIAAGEPIYAYENKDEQL
                     SIPGEMAGRGKDTFVLRVRGKSMIEDHIDDGDYVVVQSQNTARDGDIVVALLEDSRAT
                     LKRFFREKDRVRLQPANGQMQPIYTRDIQIQGKVIGVIRRLA"
     gene            7057..>7338
                     /locus_tag="VFV20_03400"
     CDS             7057..>7338
                     /locus_tag="VFV20_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEU5287423.1"
                     /translation="MPARRPSDLPLLTLRELNRALLARQMLLRRERVDPTSAIERLGA
                     LQAQWPRAPYVGLWSRLQGFDRVDLEDALSRRRVVKATLMRGTLHLASAS"
BASE COUNT         1160 a         2615 c         2465 g         1098 t
ORIGIN      
        1 gacgggggcg gcggtcacgc tacctcgcgt gggacccacc tgccccgacc acggttttcc
       61 ggcgaaattc gggcatcttg tcggtactat tgacaccaga acgtccgttc ggtttgctcc
      121 gtgcatgggg acggacgcat gaccgtcgaa acgcgcgttg accggcaggt cccgttcgat
      181 gagcgggccg agcagcacgt gctcggcagc ctgctcatcg accgcgacgc gatcttcaag
      241 atcgccgacc tcctcgccac gagcgacttc tacgtcgcgc ggcatcagcg catctacgcc
      301 tcgatctacg agctgctcca gcgccgcgag cgcatcgacc cgctcacggt gcaggtcgag
      361 ctcgcgcgac gcgaagagct cgaccgcgtg ggcggcgccg cgtacctgag gacgctcacc
      421 gatctcgtgg cgaatgccgt ggagatcgag cgccacgcgc gagtggtgcg cgaccgcgcc
      481 atcctgcggc gcctgctgaa cgcggcgacg cagatcgccg ccgacgcgta caccgagccg
      541 acggacatcc cgctcaccct cgacagggcg gagcagcgca tcttcgcgct gcgcgacgac
      601 accaccaacg cgcagctgcg gcacatccag accgcgcttc aggacaactt ccagcacctc
      661 accgagcgga tggaccggcc ctacgaggtg tccggcatcc cgagcggctt ccgcgagctc
      721 gacttcttca ccgagggctt cacgcagggc gatctcgtcg tgctcgccgc gcggccctcc
      781 gtcggcaaga ccagcctcgc gctggcgatg gcgttcaaca tcgcgaagcg cggccacccc
      841 accctcgtgt tctcgctcga gatggacgcc aagcagatcg tctcgcgctt cctcgccatg
      901 aactcgcgca ccgacctctt ggcgctgcgc accggcaacg tgttcgacac cgaggcggcg
      961 agcgcgctcg gcggcgtgcc gatcctcatc gacgacacgc ccggcctctc gatcatggag
     1021 ctgcgcacga aggcgcgacg cgccagcacc aacgaccgcc tcgaggtgat catcgtcgac
     1081 tacatccagc tgatgcgcac cggcggcgac gaggagaacc gcgtccagga gatcgcgacc
     1141 atcacgcgca acctcaagtc gcttgcgcgc gagctgaacg tcgtgatcat cggcctgtcg
     1201 cagctctcgc gcgccgcggg cgactccgga ctggagccga agctctcgac gctccgtgag
     1261 tcgggcgcga tcgagcagga cgccgacatc gtgctcatgc tctggaagga caaggaagac
     1321 acaccgcccg gcgcgccccg cctcatccac ggcagcgtcg cgaagaaccg caacggcccc
     1381 accggcatct tccagctgct cttcgcgggc gagcaggcgc gcttcttcag caaggccgcc
     1441 gacgaccaga tgccgatatg acgatcgacg tcgaacgaag cgcgcccaag ccaccgccgg
     1501 agaaacccga ggcggaggcc agcctcatcg ggtcgatcct catcgacgcg cacatctacg
     1561 aggagaccgt gcgcggccgc atcgctcccg cggagctgtc gcgcgagtcg caccgcctgg
     1621 tcctcgccgc gatcgagcgc gtgtgggagc gcggcgagcc gctcggcttc caaagcgtgc
     1681 agtccgacct cgcggcgcgg ggcgagctcg acgcggtcgg cggggcgcag ttcctcgtcg
     1741 gtctcatgca gaacgtaccc accgtcgcgc acgccgagac gtacgcgacc atcgtcgagc
     1801 gcaccgcgct cctgcgccgg ctcatcggcg cggcgaacga gatcgcccac ctcgcgctga
     1861 cgcgcgagga cgcgcggagc gccgtcgagg acgcgcagga gctgctcttc gccgtcagtg
     1921 agtcgaacct ccaccgtgac gtcgtcccgc tcgagctcgc gctgcggcgc ttcgcggacc
     1981 agatcaccgc gcgcgccgac gaccaccacg agggcgtgcc cacgggcttc gcctcgctcg
     2041 acgcgaagac gggcgggctg cagccgaacg acctcatcct gctggccggc cggcccggcc
     2101 tgggcaagac gagcttcgcg ctgaacatcg tgtggaacgc cgccgcggtg gagcgaaaga
     2161 cgtgcgcggt cttctcgctc gagatgagcg agatgcaggt cgtgcagcgc ctcatctcca
     2221 tgacgagcga gatcgacggc aaccgcatgc gccgcggtcg tctctcgccg gaagaattcc
     2281 gctcgatctc ggccgcgtcc gaccagctgc agaaggcgcc gatcttcgtc gaggaatcga
     2341 gccagctcac cgtgaccgac atcctcgcga aggcgcgccg gctccaggcc gagcgcggcc
     2401 tggacctcct cgtgatcgac tacctgcagc tcatcgaggg cgcgggcgac gacgagggcc
     2461 gcgtgctcga ggtcgcgcgc atcagccgcg ggctcaaggc gatcgcgcgc gagctgcagg
     2521 tgccggtcat cgcgctctcg cagctgtcac gccagatcga gacccgcggc accgagccga
     2581 tgctctctga ccttcgcgaa agtggcgctc ttgaagcgga cgccgacctg gtcatgttcc
     2641 tctggcagaa ggaccgcaag gaccgcgacg agaacgtcgt gcgcctcaag ctcgcgaagc
     2701 accgcaacgg gcccaccggc gacttcgacc tgcacttcca gtccgagctc acgcgcttcc
     2761 gcgacctcgg cgagacgcgc gtggaggcct agcccgcggc ggtcgtcacg cggttgcgac
     2821 cggcctgctt cgcctcgtag aggcgtcggt cggcctcacg gaagagatcg tcggcggtgc
     2881 ggccgtgctg cgggtacgtc gcgatgccca cgctcaccgt cgcgcggatc ggctcgcccc
     2941 acgaggtgcg gcattcgcgc gccgcgacgg aggtccgcag tcgctcggcc gtcacgagcg
     3001 cgtccttcgc ggtggtgcgc ggcaggatgg cgatgaattc gtcgccgccg aaccggccca
     3061 gcacgtcggt gaggcgcagc gacgcgctca tcgccgcggt gagctcgatc agcagccggt
     3121 tgccttcggc gtggccgtac tggtcgttca cctgcttcat gcagtcgccg tcgatgacga
     3181 ggagcgagac gtgctcgtcg tgtcggcgcg cggactcgat ctcctcgcgg agccgctgca
     3241 tcgcgtggcg cgtgttgtac gcgccggtga gctcgtcggt gaccgagagg cggcggatct
     3301 cctcgtgggc ctcggcggcg gcgatcgcga gggcggcctg gctcgcgatg gtccgcgcga
     3361 agcggaagtg gtcgtccgtg tacgagctca cgcccatctt caccaggcgc gcgacgccga
     3421 cgaggcgatc ctcgacgagg agcgggatca cgatcgcgga cttcgagtgt ctcggcgtgc
     3481 cgggcacggc gagggcgcgt gcgtcggcgc cgacatcgtc gacgcgcagg ggttctcggt
     3541 ggagcgcggc ccagccgacc atgccttcac cgagctcgag acgcatcgcg cggaggctgg
     3601 actcgtagtc gctggcatac accgcggcgt cgtacgacaa ggcggccatc cgaagcgtct
     3661 cggcccgtga gtcccagaga tgcatggcca ccgagtccga cgtcgtgatg ctgcggagcg
     3721 atccgcacac cgcgtcgagc gtgccggcaa ggtcgagggc cgcggcgcgc tgtgcgacct
     3781 cgctcaccgc ctgcagctcg gcgatgtcct tctgacgcgc ctgatgctgg cgcgcgtgca
     3841 tcagcgccat cgcgagcagg cgcgcgtagg ccgacagcac acgctcgtcg tgggccgaga
     3901 acgccgccgt gtgatcgctc tcgataccga gcaccccgag cgcgcggtcc ccggcgacga
     3961 tcgggatgca gatctccgaa cggcacgagg gcagcacccg gatgaatcgc ggatccatcg
     4021 agacgtccgg gacgttcacg acgctcgcgg tcgcgaagca tgccccggag acgccgcgac
     4081 ccggctcggt gcggaaacgc ccggcagcct cgatctcggc gagcgggccg gcgaacgcgg
     4141 cggtgtacag atcgtcgcgc gtctcgtccc gcagcaggat gtagcgagcg ccgtatccgt
     4201 actgctcgcc gaggagctcg gtgccgagcg acagcaggcg cttctcgtcg ctcgcggcga
     4261 gcaggagctc ttccgcgctg cgcatgagga gatcgaggtc cgagcgcgct gcggccgtca
     4321 tgtggggacc gtatgtcgcg cgcatgacct gaacaatcac gcgctcgtcc taggcgcgag
     4381 ctaccccgac cgctgggcgt cctcccccgt gtgggggtca cggttcaggc cggctcgatc
     4441 ccgatcgtgc gcggcctgtt cagcgcgcgg gctcgaccgc gacggcgacc ttcgcgcgca
     4501 cgtccgggag gatgcggatc tcgacctcgt gcggaccgat ggtcttcagc ggctcgccga
     4561 ggacgatctt cgttcggtcg atctcgatct tgtgttcgcg cttcagcgcg tcggccacgt
     4621 ccttgttggt gaccgagccg aacgtcttgc cctgctcgcc ggccttcaga tggaagacga
     4681 ggcgaagccc ggcgaggcgc gcggcaaggg cctcggcgtc ggcatgcgcg cggtccttct
     4741 tcgccttctc cgcgtcgcgc tcctgcgcga ggtgcttcag ctcgccggcc gtcgcctcga
     4801 tggcgagacc ccgcggcagg aggaagttct gcgcgtagcc gtccgcgaca tccttcacgt
     4861 cgccggggcg accgagtccg cggacctcgc gcttcaggat gacgcgcacg tcgctctgac
     4921 taggtcgtcg aaggagtggt gggctcggcg agctggccgt tctgctcgct cttgcgaagc
     4981 tccatctccc acgcgaggac cgatgccgcg acggcgatca cgcccatgac cgtgagcgcg
     5041 ccgacgatcg cgaggagcgc ctcgatcgcg ccgaagaatc ccccgcccga ctcctctctg
     5101 ctcatcgttc ttcgcgctcg gctcggccca cttcgtggcc tgcgcctcgc cgcctcgctt
     5161 cgctcgcgtg tggactcgct cctcgcacat gaagtgctcg tcgctcgtct ctcatgcgtc
     5221 acccccttgg tcacccttcg aacgcatccg ctccagcgta gcgcgaagct cgctcgcccg
     5281 gcccgcgagc agcgagcccg cggcgtcgag atccttcgcg agcgtgtcgt cgcgcgcggc
     5341 gatgcgagac atgtgctcct ccacgacctc gggcggcgcg ttcttgatga accaatcgag
     5401 ggcgaggatg acgacggcga cgtcgtccat ctggccgatc accgggatga agtccgggat
     5461 gatgtcgatg gggaacgcga ggtacgccgc gagcgcgccg agcacgagct tctgctgccg
     5521 cggtacccgc ggatcgcgcg cgagatccca caccaggcgt ccgtacgtcg gcagcttgcg
     5581 gacggtgtcg agcgcctcgt tcatgttgcg tggccgctga atacctctag gcaccatgga
     5641 cctccccctg cgggaaccgt gcccgcagcg cggcgatggc cgcggacgcc gccgcatcgc
     5701 ggtcggcgga ccagaacgac acgaggtaat gcgccttgcc gtccgacacg ctccgcagct
     5761 ccaccgcggg cttcggctcg gccacgaggc cggcggcctc cccgagcgcg ctgcgtatct
     5821 catccgcgcc gatcggccgc tcgcggtcgt cgacgatgag ccagagcgtg ccccggcgcg
     5881 tcgggaaccg ggtcagattg accacggcgc tcgtcatgaa gacgccgttc gggatgatca
     5941 cctcccggtt gtccgcggtc ttcagcatcg tcgtgcggaa ggcgatctct tcgacgacgc
     6001 cgacctgccc gccgacctcg atcgtgtccc cgatgcggaa cggccgttcc acgaggaccc
     6061 agatgccggc gaagaagttc ttcaggatgt cctgcaggga caggccgatc acgatgccga
     6121 gcacgctgaa cgaggtgagg atccagccga acgcgtcgcg ggtatagatc gcgagcacgc
     6181 cgaggacgcc caccaggatg acgaggacct gggcgaggtt cccgaggagg atcgtgacgt
     6241 tcgcctgggc ccggttccgg gcgagcatcc gcatcgtcgc ccgtcggacc cctcgcgcga
     6301 gcaggagggc cgccaccacc acgaggagcg cccacacggt gcgcatcccg tagtccagga
     6361 tcagggccga atcgaccggc atcgtgcgaa tcatgcccgc ctcttgacat gagaacatcc
     6421 gttcggtatg gtccaagcgt gacgaaacag gaccagcagc gagccgagcg catcctcgac
     6481 ttcatccgcg agcggatcgc cgagcagggc tacccgccga cggtccgcga gatcgcggag
     6541 gcggtcggac tcgcctcgac ctccgccgtg caccaccacc tcatgaagct cgagcgcgag
     6601 ggcaagctgc agaaggacgc gacacggtcg cgggccctca ccatccccgg atcgctcggc
     6661 ggtcgtgtgg tccaggcccc catcgtcggc gagatcgccg ccggcgagcc gatctacgcc
     6721 tacgagaaca aggacgagca gctctcgatc cccggcgaga tggccggccg cggcaaggac
     6781 acgttcgtgt tgcgcgtccg tggcaagtcg atgatcgaag accacatcga cgacggcgac
     6841 tacgtcgtcg tgcagtcgca gaacacggca cgcgatggcg acatcgtcgt cgcgctcctc
     6901 gaggacagcc gcgcaacact caagcgcttc ttccgtgaga aggaccgcgt ccggctgcag
     6961 ccggccaacg gacagatgca gccgatctac acccgtgaca tccagatcca agggaaggtc
     7021 atcggcgtca tccgtcgcct cgcctagcat cgcgcgatgc ccgcgcggcg gccgtcggac
     7081 cttccgctcc tcacccttcg cgagctgaat cgcgcgctcc tcgcgcgaca gatgctgctg
     7141 cggcgggagc gtgtggaccc aacgagcgcg atcgagcgcc tcggcgcgct ccaagcgcag
     7201 tggccgcgag ctccatacgt gggtctctgg tcgcggctcc aaggattcga tcgcgtggac
     7261 ctcgaggatg cgctgagccg gcgccgcgtg gtgaaggcga cgctcatgcg cgggacgctg
     7321 catctggcga gcgcctcc
//
LOCUS       DASTZR010000151         3147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1261971, whole genome shotgun sequence.
ACCESSION   DASTZR010000151 DASTZR010000000
VERSION     DASTZR010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261971"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1274
                     /locus_tag="VFV20_03405"
     CDS             <1..1274
                     /locus_tag="VFV20_03405"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006142848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU5287424.1"
                     /translation="DVAFEDREVDTEPKDLSAPAPASHRAARRRGEGRREEREPESGE
                     DARHGYAREAQPMPTDRAAFAERRRRASAALSARGLSALLLAPGSDLTYLTGYRIFST
                     ERLTCLVLPAEGEATLVLPELESPRATAAAPDLGQRTWRETDDPYTLAASLVRAPGDV
                     AVSDQMWALFTLRLQHALGGRRFDLASTITRELRMRKDAAERDALRAVSAAADRAYAR
                     ILERDFAGRRERDVAADLGALLREEGHDEVAFTIVASGPNGASPHHETSERVIGTGET
                     IVCDFGGNRRSYCSDITRTVHVGAPDPETVRVHDTVRRAQEAGYAAARTGATAESVDA
                     AARRVIDDAGYGTYFIHRTGHGIGLDGHEHPYLVAGNTERLEPGMAFSIEPGIYLPGR
                     FGVRIEDIAIIGDDGAALPLNHADHALAIVR"
     gene            1274..1642
                     /locus_tag="VFV20_03410"
     CDS             1274..1642
                     /locus_tag="VFV20_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287425.1"
                     /translation="MRVAIATLVAGLAQLFLYLRLAGSEGSGSLLALVYIVLATLGAG
                     WFAGVRPALAGGLSVVLAAALYAAVIYMGPAGVGMAPPDAFGNAIGIVATFWPYIAIG
                     AIAGALGGTLRTRVVGARRS"
     gene            1642..2016
                     /locus_tag="VFV20_03415"
     CDS             1642..2016
                     /locus_tag="VFV20_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287426.1"
                     /translation="MRTRLVASIVAATVGLAFWWALTEPLPIAPPILLAVPGLVLFCA
                     GLIAGRLGAIASPLALLFSLLVGSIIATQLHQYFAPATAPISSFGGFIALSFPELALP
                     LVAAVVLGAAGGFLGERLMPAR"
     gene            2027..2452
                     /locus_tag="VFV20_03420"
     CDS             2027..2452
                     /locus_tag="VFV20_03420"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU5287427.1"
                     /translation="MSRRIGAAAVISDDRRRVLLVRHTYGERNWELPGGIGNDGESAD
                     ATAVREVREETGLEVAAERLTGIYWEPAADMHHFAFVCRRTMRTEARPDMDENDEVRW
                     CDRSALPRPISDFTVRRIDDALDGNPAEIHEIGPRQWIR"
     gene            complement(2549..>3147)
                     /locus_tag="VFV20_03425"
     CDS             complement(2549..>3147)
                     /locus_tag="VFV20_03425"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEU5287428.1"
                     /translation="NVVALVQTNTKRMLAYSSIAHTGYILAAVAATTAGPAAGAAVLF
                     YVFAYGLMNLGAFACLLYVDLEGTRGATLNELNGFARRHGLGALVFAVFLVSLTGIPP
                     TVGFVAKFLVIQPVLDAGFAWLAVILALNAVLAAFYYLRVVVHMYMYDPESGAPVLVS
                     SRLLSISLGVSAVAVVVLGILPNSLYQWALNAAAPIVR"
BASE COUNT          456 a         1127 c         1125 g          439 t
ORIGIN      
        1 tcgacgtcgc cttcgaggat cgcgaggtcg ataccgagcc gaaggatctg tcggcgccgg
       61 cgccggcatc acaccgggcg gcgcgaagac gaggagaagg ccggcgcgaa gaacgcgagc
      121 ccgagagcgg cgaggacgcg cgacatggct atgctcgcga agcacagccg atgccgacag
      181 atcgggccgc cttcgccgag cgtcgacgac gagcgagcgc cgcgctcagc gcgcgcggcc
      241 tgtcggcgct gctcctcgcc cccgggtctg acctgaccta cctcaccggc taccgcatct
      301 tctcgacgga gcgcctcacc tgcctcgtcc tccccgccga gggcgaggcc acgctcgtcc
      361 tccccgagct cgaatcgccg cgcgcgacgg cggccgcgcc cgaccttgga cagcggacct
      421 ggcgcgagac cgacgacccg tacacgctcg ccgcatcgct ggtgcgcgcg ccgggggatg
      481 tcgccgtctc cgaccagatg tgggcgctgt tcacgctgcg gctgcagcac gcgctcggcg
      541 gccggcgctt cgatctcgcg tcgacgatca cgcgcgagct gcggatgcgg aaagacgccg
      601 cggagcggga cgcgctgcgc gcggtgtccg ccgccgccga tcgcgcgtac gcgcgcatcc
      661 tcgagcgcga cttcgccgga cggcgcgagc gcgacgtggc ggccgacctc ggcgcgctgc
      721 tgcgcgagga gggtcacgac gaggtcgcgt tcaccatcgt cgcctcgggg ccgaacggcg
      781 cgtcgccaca ccacgagacg agcgagcgcg tcatcggcac gggcgagacg atcgtgtgtg
      841 acttcggggg caaccgcagg tcctactgct cggacatcac gcgcacggtg cacgtcgggg
      901 cgcccgatcc ggagacggtc cgcgtgcatg acaccgtccg gcgcgcgcag gaggcgggct
      961 acgcggcggc acgcaccggc gcgacggccg agtcggtcga cgccgccgcg cgacgggtca
     1021 tcgatgacgc cggctacggc acgtacttca tccatcgcac cggtcatggc atcggcctcg
     1081 acggccacga gcacccgtac ctcgtcgccg gcaacaccga gcgcctcgag ccgggcatgg
     1141 cgttctcgat cgagccgggc atctacctgc cgggacgctt cggcgtccgc atcgaggaca
     1201 tcgcgatcat cggtgacgac ggtgcggccc tgccgctgaa ccacgcggac cacgcgctcg
     1261 cgatcgtgcg ctagtgcgcg tggcgatcgc gacgctcgtc gcgggcctcg cgcagctgtt
     1321 cctttacctg cgcctcgccg gcagcgaagg gagcggctcc ctcctggccc tcgtgtacat
     1381 cgtgctcgcg acgctcggtg ccggctggtt cgccggcgtt cggcccgcgc tcgccggcgg
     1441 gctttcggtc gtgcttgccg ccgccctgta cgccgccgtc atctacatgg gacccgccgg
     1501 cgtcggcatg gcgccgccgg acgcgttcgg caacgccatc gggatcgtcg cgaccttctg
     1561 gccctacatc gccatcggcg cgatcgccgg cgcgctcgga gggacgctgc gcacacgggt
     1621 cgtgggagcg cgccggagct gatgcggacg cggctcgtcg cgtcgatcgt cgcggcgacc
     1681 gtcgggctcg cgttctggtg ggcgctcacc gagccgctgc ccatcgcgcc accgatcctg
     1741 ctcgcggtcc caggtctggt gctgttctgc gccggtctga tcgcgggacg gctcggcgcg
     1801 atcgcgtcgc cgctggcgct cctcttctcg ctcctcgtcg gcagcatcat cgcgacgcag
     1861 ctgcaccagt acttcgcgcc ggcgacggcg ccgatctcct cgttcggcgg cttcatcgcg
     1921 ctctcgttcc ccgagctcgc gctcccgctc gtcgcagcag tcgtccttgg cgcggcgggc
     1981 ggcttcctgg gcgagcgcct catgcccgcg cggtagtctc ccgcgcatgt cgcgacggat
     2041 cggagcggcc gcggtcatca gcgacgatcg ccggcgcgtg cttctcgttc gacacacgta
     2101 cggcgagcgc aactgggagc tgccgggagg catcggcaat gacggcgagt ccgccgacgc
     2161 caccgcggtg cgcgaggtgc gcgaggagac gggactcgag gtcgccgccg agcgattgac
     2221 cggcatctac tgggagcccg cggccgacat gcaccacttc gcgttcgtgt gccggcggac
     2281 gatgcggacc gaggcgcggc cggacatgga cgagaacgac gaggtgcgct ggtgtgaccg
     2341 cagcgcgctt ccgcgtccga tcagcgactt cacggtgcgc cggatcgacg acgcgctcga
     2401 cgggaacccc gccgagatcc acgagatcgg gccgcgtcaa tggatccgct gatcgggctg
     2461 ggcgtgatcg cgatcctctg acgcacgctc gcgtcggcga tgcggaggga gtcttgacga
     2521 ccggcgagga ccgagccgtt cgacctcttt agcgaacgat cggcgcggcg gcgttgagcg
     2581 cccactggta cagcgagttc ggcaggatgc ccagcacgac gacggcgacg gccgagaccc
     2641 cgagcgagat cgacagcagg cgcgatgaca cgagcacggg cgcgccgctc tcggggtcgt
     2701 acatgtacat gtggacgacg acgcgcaggt aatagaacgc cgcaagcacc gcgttgagcg
     2761 cgaggatcac cgcgagccat gcgaagcccg cgtcgagcac cggctggatc accaggaact
     2821 tcgccacgaa tccgacggtc ggcgggatgc cggtgagcga cacgaggaac acggcgaaga
     2881 cgagcgcgcc gagcccgtgg cgccgggcga agccgttcag ctcgttcagc gtcgcgccac
     2941 gcgtgccctc gaggtcgacg taaaggaggc acgcgaacgc gccgaggttc atgagtccgt
     3001 acgcgaagac gtagaagagg accgccgcgc ccgcggccgg gccggccgtc gtcgccgcga
     3061 cggcggcgag gatgtagccg gtatgcgcga tgctcgagta cgcgagcatc cgcttcgtgt
     3121 tcgtctgcac cagcgcgaca acgttcc
//
LOCUS       DASTZR010000152         4446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1027434, whole genome shotgun sequence.
ACCESSION   DASTZR010000152 DASTZR010000000
VERSION     DASTZR010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027434"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VFV20_03430"
     CDS             complement(<1..453)
                     /locus_tag="VFV20_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5287429.1"
                     /translation="MSRPLVVSELRKRYGDLPALGGVTFEVAPSEIVAVVGPSGCGKS
                     TLLRLVAGLDTRYHGEIRVGDEKVCGPHPSIGIVFQEPRLFPWLKVADNVAFGLQDRE
                     SKAARSHVRDALEAVGLADFGGALPKELSGGMAQRVALARSLVTEPQVL"
     gene            complement(450..1199)
                     /locus_tag="VFV20_03435"
     CDS             complement(450..1199)
                     /locus_tag="VFV20_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5287430.1"
                     /translation="MPWILPAVIVAVWWGSVSLGIFAVYQVPPPPVVLDAAIALWRRG
                     LLQQDLIATLARVVEGFVLGSLVAIVLGTITGLSRRAENALEPTLQALRTVPTLAWAP
                     LLLIWLGIDEPPKVTLVAIGAFFPVYVNLVAGITGVDRKLVEVARVFDLGPREIARRV
                     ILPAALPELLTGLRLGATQAWLFVVVAEFFGASAGLGFRLTDSQQSTRVDLMFVALVA
                     LALLGRLTDALIRALERRVLRWRDTIGEVRA"
     gene            complement(1268..2257)
                     /locus_tag="VFV20_03440"
     CDS             complement(1268..2257)
                     /locus_tag="VFV20_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042918 - alkanesulfonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU5287431.1"
                     /translation="MTRAAAILASLAILVVACGGATGPSATGDADTGLTEIRVDYATY
                     SPPSLVLKQQGLLEEAFPGVSIKWVYSAGSSNALNFLQGASVDFGSTAGSAALLSRAN
                     GNPIKVVYIYEQPEWTALVVAKDSTAKSITDLKGKKIAAQKGTDPYFFLLRTLQQAGL
                     TQADIQYVNLPHADGRTAVERGQVDAWSGLDPHMAASELQAGSRLIYRNKDFNTYGFL
                     NAREEFVKKYPQTTKKLIALYEKAKQWVIDNPDGAAKILADAAQLPLDVAKRELVDRM
                     NFKTTGIPGEAHAAVLRAVIPTIEKEQLANPGANVAKAVDDLIDGTFAKAVIK"
     gene            complement(2512..2733)
                     /locus_tag="VFV20_03445"
     CDS             complement(2512..2733)
                     /locus_tag="VFV20_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011374805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEU5287432.1"
                     /translation="MRILDLVATALLVIGGINWGLVALAKFDLVAYVTGQSFGDVNLA
                     SAIVYGLVALSALWVGARAVSQPRVFSAA"
     gene            2844..3704
                     /locus_tag="VFV20_03450"
     CDS             2844..3704
                     /locus_tag="VFV20_03450"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU5287433.1"
                     /translation="MIRIGELAKRAGVSPELVRAWERRYALLSPRRTEGGYRLYDDDA
                     VSRIRRMRELIAGGMSTAQAADLAKAGAAAPAVAAPIPSELTAALAQALRSLDEASAH
                     AAVDRLLSTVTVESFITDAVLPILRDIGERWSVRRTTIGHEHFATTVLRGRLLGLARG
                     WDNGPGPLVALACLPGELHDVGLVAFGIVLSRSGLRVLYLGQDTPLDSVADLAVGQGC
                     VLAVIASVESARFDEHASGLRKLARSLPVAIGGAGANAAIAKRTGARLLPEDPVAAAR
                     EIARAQWSHA"
     gene            3701..>4446
                     /locus_tag="VFV20_03455"
     CDS             3701..>4446
                     /locus_tag="VFV20_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5287434.1"
                     /translation="MSSRWLGLACLSLGVAMIIVDATIVNVAVPALVADLGLDSTQAE
                     WVNTTYALVFAALLVTLGRVGDLVGRRATYLAGTVLFAVASMAAGLAPSGELLIAARV
                     AQGLAGAAMLPATQSILNTTFRGHDRAIAFGVWGATIGGVAAVGPLLGGWLTTNLSWR
                     WAFYVNVPIAIIAIVGTLITVRESKDETAARGWDPLGFVLVTAGLSSFVFGLVEGRLY
                     GWLAPARPFGVFGLEWPSTTVSVIPFMLAGG"
BASE COUNT          651 a         1538 c         1576 g          681 t
ORIGIN      
        1 caggacctgc ggctcggtca cgagcgagcg cgcgagcgcg acgcgctgtg ccatcccgcc
       61 cgagagctcc ttcggtaggg cgccgccgaa gtccgcgagg ccgaccgctt cgagcgcgtc
      121 acgcacatgc gaacgcgcgg ccttgctctc gcgatcctga agcccgaacg cgacgttgtc
      181 ggcgaccttc agccagggga agaggcgcgg ttcctggaac acgatcccga tcgatggatg
      241 cgggccgcag accttttcgt cgccgacgcg gatctccccg tgatagcgcg tgtcgagccc
      301 ggcgacgagg cgcaggaggg tcgacttgcc gcagcccgac gggccgacga ccgccacgat
      361 ctcgctcggc gcgacttcga acgtgacgcc gccgagcgcg ggcagatcgc cgtagcgctt
      421 gcgcaattcg ctgacgacga gcggccggct catgcgcgca cctcgccgat ggtgtcgcgc
      481 cagcgcagca cgcggcgctc gagcgcgcgg atcagcgcgt cggtgagccg gcccagcagc
      541 gcgagcgcga cgagcgcgac gaacatgagg tcgacgcgcg tggactgctg gctgtcggtg
      601 agacggaagc cgaggcccgc cgacgcgccg aagaactccg ccaccaccac gaagagccac
      661 gcctgcgtcg cgccgaggcg caggccggtg agcagctcgg gcagcgcggc tgggaggatg
      721 acgcgccgcg cgatctcgcg gggtccgagg tcgaagacgc gcgcgacctc caccagcttg
      781 cgatcgacgc cggtgatgcc ggcgacgagg ttcacgtaga ccggaaagaa ggcgccgatc
      841 gcgacgaggg tgaccttcgg cggctcgtcg atgccgagcc agatgaggag gagcggcgcc
      901 cacgccagcg tcggcacggt acgcagcgcc tgcagcgtcg gctcgagcgc gttctccgcg
      961 cgccgcgaca gaccggtgat cgtgccgagc acgatcgcga cgagcgatcc gaggacgaag
     1021 ccctccacga cgcgcgcgag cgtcgcgatg aggtcctgct gcaggaggcc gcggcgccaa
     1081 agtgcgatcg cggcgtcgag cacgaccggc ggcggcggca cctggtacac ggcgaagatg
     1141 ccgagcgaga ccgagcccca ccacaccgcg acgatgaccg ccgggaggat ccacggcacg
     1201 agggagggcc accgccgccg ggcagtgacg gcggcggtgg ctcgcagggc ggtacccagg
     1261 ggcgtggcta tttgatgacc gccttcgcga aggtcccgtc gatgaggtcg tccacggcct
     1321 tcgcgacgtt cgcgccggga ttggcgagct gctccttctc gatcgtcggg atcaccgcgc
     1381 gcagcaccgc cgcgtgcgcc tcaccgggga tgccggtggt cttgaagttc atgcgatcga
     1441 cgagctcacg cttggcgacg tcgagcggca gctgcgcggc gtcggcgagg atcttcgccg
     1501 cgccgtccgg gttgtcgatc acccactgct tcgccttctc gtaaagggcg atgagcttct
     1561 tcgtcgtctg cggatacttc ttcacgaact cctcgcgcgc gttcaggaag ccataggtgt
     1621 tgaagtcctt gttgcgatag atgagccggc ttccagcctg gagctcgctc gcggccatgt
     1681 gcgggtcgag gcccgaccac gcgtcgacct gcccgcgctc gacggccgtg cgtccatccg
     1741 cgtgcggcag gttcacgtac tggatgtcgg cctgcgtgag accggcctgc tgcagcgtgc
     1801 gcaggaggaa gaagtacggg tcggtgccct tctgcgccgc gatcttcttg cccttcagat
     1861 ccgtgatgct cttcgccgtc gagtccttcg cgaccacgag cgccgtccac tcgggctgct
     1921 cgtagatgta cacgaccttg atcgggttgc cgttcgcgcg ggacagcagc gccgcggatc
     1981 cggccgtcga gccgaagtcg acggaggcgc cctggaggaa gttgagcgcg ttgctcgatc
     2041 cggccgagta cacccacttg atcgacacgc cggggaaggc ctcctcgagg aggccctgct
     2101 gcttcagcac cagcgagggc ggcgagtacg tcgcgtagtc gacgcggatc tcggtgaggc
     2161 cggtgtcggc gtcgcccgtc gcgctcggac cggtcgcgcc gccacacgcg accacgagga
     2221 tcgcgaggct cgccaggatc gccgcggcgc gtgtcatgcc gacctccttc aaatgaagag
     2281 gcccccgatc tctcgggggc ctctgtgctt cggtgtttgt gcgcgacgct tacgcgacgt
     2341 tcaccagagc gacacaaagg ccccgggaca tgggggccat cgcgcaggcg cacatgcaga
     2401 tcgcgcgctg tgtcatgagg cgaacaacgt aggcggcggc tccgcgtggg tcaatgcgag
     2461 ggcgcgaccg gtggtgcacc gtgcacgacc ggtacgcgcc acgggtgacg ctcaggctgc
     2521 cgagaagacg cgtggctggc tcacggcgcg ggcgccgacc cacagggccg agagggccac
     2581 caggccgtag acgatcgccg acgcgaggtt gacgtcgccg aagctctgcc cggtcacgta
     2641 cgcgacgagg tcgaacttcg cgagcgccac gagaccccag ttgatgccac cgatgacgag
     2701 gagcgccgtt gcgacgaggt cgaggatgcg catgagattc ccctttcgcc gggcatttcc
     2761 ggctgtcaaa tgtttgtaga aggaccgtag cggagtctgt acagacttgt caagagtctg
     2821 tcccatgttt gtataatgac ctcgtgatcc gtatcgggga gctcgcgaag cgcgcgggcg
     2881 tgagtccgga gctggtgcgc gcgtgggagc ggcgctatgc gctgctctcc cccaggcgca
     2941 cggagggcgg gtatcgcctg tacgacgacg acgccgtctc ccgcatacgg cgcatgcggg
     3001 agctcatcgc cggcggcatg agcactgccc aggcggccga tctcgcgaag gccggggcgg
     3061 ccgcaccggc ggtggcggcc ccgatccctt cggagctcac cgcggcgctc gcgcaggcgc
     3121 ttcggtcgct cgatgaggcc tccgctcacg ctgcggtcga ccggctcctc agcacggtga
     3181 ccgtcgagtc gttcatcacg gacgccgtcc tgccgatcct ccgtgacatc ggcgaacgct
     3241 ggtcggtacg gcgcaccacg atcggccacg agcacttcgc tacgaccgtg ctgcgcggcc
     3301 gcctcctcgg gctggcgcgc ggctgggaca acggccccgg tccgctcgtc gccctcgcgt
     3361 gtctgcccgg agagctgcac gacgtcgggc tcgtcgcgtt cgggatcgtc ctctcgcgga
     3421 gcggccttcg cgttctgtat ctgggacagg acacgccgct cgacagcgtc gccgacctcg
     3481 ccgtgggcca gggctgcgtc ctcgcggtga tcgcgagcgt cgagtccgcc cgcttcgacg
     3541 aacacgcgag cgggctgcga aagctcgcgc gctcccttcc cgtcgccatc gggggcgcgg
     3601 gtgcgaacgc ggccatcgcg aagcgcaccg gcgcccgcct gctgcccgag gacccggtcg
     3661 ccgcggcgcg cgagatcgcc cgagcgcagt ggtcgcacgc atgagctcgc gctggctcgg
     3721 cctcgcctgt ctcagcctgg gtgtcgcgat gatcatcgtc gacgccacca tcgtgaatgt
     3781 cgcggtcccg gcgctcgtcg cggacctcgg cctcgacagc acacaggccg aatgggtcaa
     3841 cacgacctac gcgctggtct tcgcggccct cctcgtcacg ctcggccgcg tcggcgatct
     3901 cgtgggacgg cgcgcgacgt acctcgccgg tacggtcctc ttcgcggtcg catcgatggc
     3961 cgccggactc gcaccgagcg gtgagttgct catcgccgca cgcgtcgcgc agggcctcgc
     4021 cggcgcagcg atgctgccgg cgacgcagtc gatcctcaac acgacgttcc ggggacacga
     4081 ccgggccatc gcgttcggcg tctggggcgc gacgatcggt ggggtcgccg cggtcgggcc
     4141 gctcctcgga ggctggctca ccacgaacct gtcgtggcgc tgggccttct acgtgaacgt
     4201 accgatcgcg atcatcgcca tcgtcggaac gctcatcacc gtgcgcgagt caaaggacga
     4261 gacggcggcg cgcggctggg acccgctcgg cttcgtgctc gtgaccgccg ggctgtcgtc
     4321 gttcgtcttc ggcctcgtcg agggccgcct ctacggctgg ctcgctcccg cgcgcccctt
     4381 cggggtgttc ggcctggagt ggccgtcgac gaccgtctcg gtcatcccct tcatgctcgc
     4441 aggggg
//
LOCUS       DASTZR010000153         2213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_217403, whole genome shotgun sequence.
ACCESSION   DASTZR010000153 DASTZR010000000
VERSION     DASTZR010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217403"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VFV20_03460"
     CDS             <1..483
                     /locus_tag="VFV20_03460"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5287435.1"
                     /translation="GTAFRAPAEEFPEERFDAIVALNLKGTYLPCQAFGRKMLEARSG
                     SIVNVASIGGFIAYPHASAYLSTKGGIVNLTRGLALEWVDRGVRVNAIGPGLCDSPLT
                     RARAKQSSVTSDFIQARMLRRELILPRELVGAAIFLASDASLKVTGHTLMVDDGYLVA
                     "
     gene            574..1983
                     /locus_tag="VFV20_03465"
     CDS             574..1983
                     /locus_tag="VFV20_03465"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU5287436.1"
                     /translation="MSESIERGDAATLDAIEGLVTGAMGRDEFIKRLTYLGFSASAIG
                     GMLAAAGKADAAAPKPFAGQTVRILVVAEGDDKGVRDKIATIKQRFGIDLKMTALPVG
                     PLIEKANQNLKAPTSSFDIIQVLGFSVSQMVGGGFFEPLNKYLAKAPAGYDFKDFPPG
                     QLKYTGYYDTRKQQFGGNSLYLIPGLHAGSVILFYRKDLFKAAGLKAPRSWPAYLQAA
                     RRLHRDGVAGNSMIAKSGDVSMFLVDWYTRFANMGGKLMSGSPQAKTFQPRLTSPQAV
                     AALQHMVDCVEFASDGVLSYDFTLSVDAFSAGKTAMMLMWSTIAGPVYNPKSSKVARQ
                     VDVAIPAGPGKAVRGGWGVGIPKNAKNKDAAWQVITYLTSKEWDRYQTATYQTDPTRR
                     STFKALAGRYPYLKVAGQEIERAQILEIASVPETFELITIAAEEFSAALNGSSSAAEA
                     CKKANDRWTQVLKRGGHLK"
     gene            2003..>2213
                     /locus_tag="VFV20_03470"
     CDS             2003..>2213
                     /locus_tag="VFV20_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU5287437.1"
                     /translation="MAATAGELQPGSRDFAPAQRRGEALLLLGPAVLYLVAFSVFPLV
                     VSLGRSFQRYDPRADSWTWIGLGNYR"
BASE COUNT          368 a          749 c          746 g          350 t
ORIGIN      
        1 ggcacggcct tccgagcgcc ggccgaggag ttcccggagg agcggttcga tgcgatcgtc
       61 gcgctcaacc tgaagggcac gtacctgccg tgccaggcgt tcggacgcaa gatgctcgag
      121 gctcggtcag gcagcatcgt caacgtcgct tcgatcggcg gcttcatcgc gtacccgcac
      181 gcgagcgcgt atctctcgac gaagggcggc atcgtgaacc tgacgcgcgg gcttgcgctc
      241 gagtgggtcg accggggcgt gcgcgtgaac gcgatcggcc ccggactgtg cgactcgccg
      301 ctcacccgcg cgcgcgcgaa gcagtcatcc gtcacgagcg acttcatcca ggcgcggatg
      361 ctgcgtcggg agctgatcct gccccgtgag ctcgtaggcg ctgcgatctt cctcgcgagc
      421 gacgcttcgc tgaaggtgac ggggcacacc ctgatggtcg acgacggcta cctggtggcg
      481 tgaggcccgt tcgttgacct tctaccactc gagtagtaag gtcgcgcgcg acttgaccgc
      541 ggtgcgacgg accgctctac gaaggaggag ggaatgagcg agagcatcga acgcggcgac
      601 gccgcgaccc tggatgcgat cgagggtctc gtcaccggtg cgatgggccg tgacgagttc
      661 atcaagcggc tcacgtacct cggcttctcg gcgagcgcca tcggcgggat gctggccgcg
      721 gccggcaagg ccgatgccgc ggcgcccaag ccgtttgccg ggcagaccgt acggattctt
      781 gtcgtcgccg agggcgacga caagggcgtc cgcgacaaga tcgcgacgat caagcagcgc
      841 tttgggatcg acctgaagat gacggccctg ccggtcggcc cgctgatcga gaaggcgaac
      901 cagaacctga aggcgcccac ctcgtccttc gacatcatcc aggtgctggg cttctcggtc
      961 tcccagatgg tcggcggcgg cttcttcgag ccattgaaca agtacctcgc caaggcaccc
     1021 gcgggctacg acttcaagga cttcccgcca gggcagctca agtacaccgg ctactacgac
     1081 acgaggaagc agcagttcgg cggcaattcc ctgtacctga tcccgggcct gcacgcaggc
     1141 tcggtgatcc tcttctaccg caaggacctg ttcaaggccg ccggcctgaa ggcgccacgg
     1201 tcgtggcccg cgtacctcca ggccgcacgg cggctgcacc gcgacggtgt cgccggcaac
     1261 agcatgattg ccaagtccgg cgacgtgtcg atgttcctcg tcgactggta cacacggttc
     1321 gccaacatgg ggggcaagct gatgagcggg tcgccgcagg cgaagacctt ccagccgcgg
     1381 ctgacgagcc cgcaggcggt cgccgccctg cagcacatgg tcgactgcgt cgagttcgcc
     1441 tcggacggcg tgctctcgta cgacttcacg ttgtccgtcg acgcgttctc cgcagggaag
     1501 acggcgatga tgctgatgtg gtcgaccatc gccggcccgg tctacaaccc caagtcgtcc
     1561 aaggtcgcgc ggcaggtcga cgtcgcgatt ccggcggggc cgggcaaggc cgtccgcggc
     1621 ggctggggcg tcggcatccc gaagaacgcg aagaacaagg acgccgcgtg gcaggtgatc
     1681 acgtacctca cctccaagga gtgggaccgc taccagaccg cgacgtacca gaccgatccg
     1741 acccgccggt cgaccttcaa ggcgctcgcc ggccgatacc cgtacttgaa ggtggccgga
     1801 caggagatcg agcgcgccca gatcctcgag atcgcctcgg tccccgagac gttcgagttg
     1861 atcaccatcg cggccgagga gttcagcgcc gccctcaacg gctcgtcgtc tgcggcggag
     1921 gcctgtaaga aggccaacga ccgctggacg caggtgctga agcgaggagg tcacctcaag
     1981 tagcggcgcc ggcgcgacgg tgatggctgc caccgccggc gagctccagc cgggcagcag
     2041 ggacttcgcc cccgcccagc ggcgcggcga ggccctgctg ctgctcggtc ccgcggtcct
     2101 ctacctcgtc gccttctcgg tcttcccgct cgtcgtcagt ctcggccgct cgttccagcg
     2161 ctacgacccg cgggccgact cgtggacctg gatcgggctc ggcaactacc ggg
//
LOCUS       DASTZR010000154         2300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_740506, whole genome shotgun sequence.
ACCESSION   DASTZR010000154 DASTZR010000000
VERSION     DASTZR010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740506"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VFV20_03475"
     CDS             complement(<1..609)
                     /locus_tag="VFV20_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEU5287438.1"
                     /translation="MKSVVVGTAGHIDHGKSSLVLALTGTDPDRLKEEKARGITIDLG
                     FAHARVGDVELAFVDVPGHERFVKNMLAGAGGIDAVLLVIAADESVMPQTREHFAICR
                     LLQIPRGVIALTKSDLVDADTLELAALEARALVADSPLASAPIVAVSSRTGAGLDELR
                     AALAGVAAAATPRAAAGPPRLPIDRVFSMRGFGTVVTGTLTQG"
     gene            complement(733..>2300)
                     /gene="rnr"
                     /locus_tag="VFV20_03480"
     CDS             complement(733..>2300)
                     /gene="rnr"
                     /locus_tag="VFV20_03480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEU5287439.1"
                     /translation="LGGAVKEKDIRDRTDFRPLTTVTIDGEHARDFDDAITIDKLPNG
                     NYWLGVHIADVAHYVPEGSALDEEAYERSTSVYFPERAVHMFPSELSTGLCSLNPHVD
                     RLVQSCLMEIDRRGDVVRYEIHDGVIHSDARMTYTDVNAILTDHDPALTKKYVKLVPM
                     FESMHELFEILNARRRRRGSIDFDLKEPEIILDDEGLVEQIIALERNVAHRIIEEFML
                     VANETVAQHLEEHNVPTLYRIHETPDPLKVDEFEEFVSTLGYSVGAPANAVKPRHFQR
                     LVEKIHGKPEEKPIAFLMLRTMQKARYDAENLGHFGLAASYYTHFTSPIRRYPDLVVH
                     RTLRESRRGMSAERGEQLVEDLPEIARHTSERERRANDAERELVQWKKVRFMADKVGD
                     EFEGYITGVTAFGLFIELIEHFVEGMVHVSTMADDYYRFVERAHILRGENTGRVFRLG
                     DRVSVQVIKVDMERRQIDLGLTDILARVRESEMNRGPRRTKAEPKSERHLKAGKRKQR
                     PGKHERAVRRRGR"
BASE COUNT          319 a          760 c          808 g          413 t
ORIGIN      
        1 gccctgggtg agcgtgccgg tgacgacggt gccgaaaccg cgcatcgaga acacgcgatc
       61 gatcggcagg cgcggcgggc cggcggcggc gcgtggcgtg gccgcggccg ccacgccggc
      121 gagtgcggcg cgcagctcgt cgaggccggc gccggtccgc gacgacaccg cgacgatcgg
      181 cgccgacgcc agcggcgagt ccgcgacgag cgcccgcgcc tccagcgccg cgagctcgag
      241 ggtgtcggcg tcgacgaggt cgctcttggt gagcgcgatg acgccgcgcg ggatctgcag
      301 caggcggcag atcgcgaagt gctcgcgggt ctgcggcatc accgattcgt cggcggcgat
      361 gacgagcagg acggcgtcga tgccgccggc gccggcgagc atgttcttga cgaagcgttc
      421 gtgtccgggc acgtcgacga acgccagctc cacgtcgccg acccgcgcgt gcgcgaaccc
      481 gaggtcgatc gtgatgccgc gcgccttctc ttccttcagc cgatccggat cggtgccggt
      541 cagcgccagc acgagcgagc tcttgccgtg atcgatgtgg ccggccgtcc cgacgacgac
      601 ggacttcatt cttggcgctc gggccttcgg ccctcgctta cgcgcccttc tctacgctcg
      661 cgcttcgtcg ctcgctccgt tccgggcgcg gctgacgtcc gtctcgtcca ccaaccgaga
      721 ttgatcactt tctcaccggc cgcggcggcg caccgcacgc tcgtgtttgc cgggccgctg
      781 cttccgcttc cccgccttca gatggcgctc ggatttcggc tcggccttcg tgcgccgtgg
      841 cccgcggttc atctcggact cgcgcacgcg cgcgaggatg tcggtcaggc cgagatcgat
      901 ctgccgccgc tccatgtcga ccttgatcac ctggacgctg acgcggtcgc cgagccggaa
      961 gacgcggccg gtgttctcgc cgcgcaggat gtgcgcccgc tcgacgaagc ggtagtagtc
     1021 gtcggccatc gtcgagacgt ggaccatgcc ctcgacgaag tgctcgatca gctcgatgaa
     1081 gaggccgaag gcggtgacgc ccgtgatgta gccctcgaac tcgtcgccga ccttgtcggc
     1141 catgaaccgc accttcttcc actgcaccag ctcgcgttcg gcgtcgttgg cgcgacgctc
     1201 gcgctccgag gtgtggcgcg cgatctccgg caggtcctcg accagctgct cgccgcgctc
     1261 ggcgctcatg ccgcggcgcg actcgcgcag cgtgcggtgg acgacgagat cgggatagcg
     1321 gcggatcggc gaggtgaagt gcgtgtagta gctcgccgcc agcccgaagt gcccgagatt
     1381 ctccgcgtcg tagcgcgcct tctgcatcgt ccgcagcatg aggaaggcga tgggcttctc
     1441 ttccggcttg ccgtggatct tctcgaccag gcgctggaag tggcgcggct tgacggcgtt
     1501 ggcgggagcg ccgacgctgt agccgagcgt gctgacgaat tcctcgaact cgtcgacctt
     1561 cagcgggtcg ggcgtctcgt ggatgcgata gagcgtcggg acgttgtgct cctcgagatg
     1621 ctgcgcgacg gtctcgttcg ccacgagcat gaactcctcg atgatgcggt gcgcgacgtt
     1681 gcgctcgagc gcgataatct gctcgaccag tccctcgtcg tcgaggatga tctccggctc
     1741 cttgaggtcg aaatcgatgg agccgcggcg gcgccggcgg gcgttgagga tctcgaacag
     1801 ctcgtgcatc gactcgaaca tcggcacgag cttcacgtac ttcttcgtca gcgccggatc
     1861 gtggtcggtg aggatggcgt tgacgtcggt ataagtcatg cgtgcgtcgc tgtgaatgac
     1921 gccgtcgtgg atctcgtagc ggacgacgtc gccgcggcgg tcgatttcca tcaggcagga
     1981 ctggacgagc cgatcgacgt gcggattcag gctgcacagg cccgtcgaca gctcggaggg
     2041 aaacatgtga acggcgcgtt ccgggaagta caccgaggtc gagcgctcgt aggcttcctc
     2101 gtcgagcgcg ctgccctccg gcacgtagtg cgcgacgtcg gcgatgtgga ccccgagcca
     2161 gtagttgccg ttcggcagct tgtcgatcgt gatggcatcg tcgaagtcgc gcgcgtgctc
     2221 gccgtcgatg gtcacggtgg tgagcgggcg gaagtcggtg cggtcgcgga tgtccttttc
     2281 cttcaccgcg ccgccgagcc
//
LOCUS       DASTZR010000155         3685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1158138, whole genome shotgun sequence.
ACCESSION   DASTZR010000155 DASTZR010000000
VERSION     DASTZR010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158138"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VFV20_03485"
     CDS             complement(<1..389)
                     /locus_tag="VFV20_03485"
                     /inference="COORDINATES: protein motif:HMM:NF013673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HEU5287440.1"
                     /translation="MTLFGADAILGPLRGALQGATGDEAELWAHRRRSAITRYAKNEV
                     HQNAVADEWYVQARVAVKGAVGIASANSLEPADLRRSLADARAAAEVSIPNAEWPGLA
                     VPEAIGQPTAFDPPTAEVDARRQAEDIR"
     gene            complement(386..1225)
                     /locus_tag="VFV20_03490"
     CDS             complement(386..1225)
                     /locus_tag="VFV20_03490"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU5287441.1"
                     /translation="MLFALLMGTSACTEAPASSPTPQPQGSVLVGTTTTTQDTGLLDV
                     LVPDFERRSGWKVKLVVGGSGQILTQASRGDLDVILTHSPPDEEAFVRSGNASERRLV
                     MHNDFVILGPASDPARVKGAAVTDALRRIYDAGASFVSRGDRSGTNVKELALWRDAGR
                     DPKGRPWYLESGTGQLQNLQLAVARTAYTLSDRGTWLANRDKLPGLAVLVERGPALLN
                     VYHVTLLDPAKFPKVEQRGAKAFADYLTGRDGQALIASYGVQKYGESTFTADAGKRED
                     ELE"
     gene            complement(1393..2829)
                     /locus_tag="VFV20_03495"
     CDS             complement(1393..2829)
                     /locus_tag="VFV20_03495"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEU5287442.1"
                     /translation="MRSLETSLPDLVHLAASRGATYADARSVDREHESVSLRDGQVES
                     VTRTSDRGVGFRVIHNGAWGFAATDRTGEAAMKELVDEAFRRAEASASLRSRSITLAP
                     VAPQHGEYRSTIRRDPFAVPMRERIALLQAADEALRGPNARSRRSSIAAYRTRKRLVT
                     SEGTDVSQEIVESGAGVAVTALKAGAKPAQRYDSRLTRQAGWEFVEALDLVARAKRYR
                     DDAEATLDAPLATERPTTLVFAPEFLALLVHESCGHPTEADRVLEYEIAFAGTSFMWP
                     KDRGTLRYGSEHVSMTADATVPGGMGTFGWDDDGVPAMRTKLVDKGIFVGYLTSRETA
                     AALDMPVAIGSARAEGWQHFPIVRMVNVSLDPGPRSYPELLKGVEDGLLLEAPASYSL
                     DDKRQNFHFSTQAARVIRNGELQGYVRGVSFQSLTPEFWGRCDGVADDWELHGFLSCA
                     KGEPLQLMRVGHGAAHARFRDVPIEVRE"
     gene            2865..3401
                     /locus_tag="VFV20_03500"
     CDS             2865..3401
                     /locus_tag="VFV20_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287443.1"
                     /translation="MLASRLLAPLAVGVMCGICAGALAGLWARIAMRLVAIGVADGVG
                     ITPEFTIAGTLAIVIVGIVVGAPAGLVYGLVADRLPGPGRWRGLLYAAVLLVLVGPFF
                     FRIEEFFSTGRVLLFVPPFLLYGLVLGLALVPMRRTVTRMPLGAQAALAVTGLGALGL
                     LAFGVAGAIFGVPTGFVM"
     gene            complement(3411..>3685)
                     /locus_tag="VFV20_03505"
     CDS             complement(3411..>3685)
                     /locus_tag="VFV20_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287444.1"
                     /translation="NVGKTEHEFMAGRQPTPGKGYAEDWIAKASTNVPDHTHPGEAHT
                     GEGVRVSADWYASLNVLVPPETGEYEFACCVQGHYEAGMKGKIVVR"
BASE COUNT          470 a         1344 c         1302 g          569 t
ORIGIN      
        1 cggatgtcct cggcctgccg ccgcgcgtcc acctccgcgg tgggcggatc gaacgccgtc
       61 ggctgcccga tggcctcggg cacggcaagg cccggccatt ccgcattcgg gatcgagacc
      121 tccgccgccg cccgcgcgtc cgccagcgat cgccgcaggt cggccggctc gagcgagttc
      181 gcgctcgcga tgcccaccgc acccttcacc gcgacgcgcg cctggacgta ccactcgtcg
      241 gcgaccgcgt tctgatggac ctcgttcttc gcgtagcggg tgatcgccga gcggcgccgg
      301 tgcgcccaga gctcggcctc gtcccccgtc gcgccctgga gcgcgccgcg gagcgggcca
      361 aggatcgcgt cggcgccgaa cagcgtcact cgagctcgtc ctcgcgtttg cccgcgtccg
      421 cggtgaacgt cgactcaccg tacttctgga cgccgtagct cgcgatgagc gcctggccgt
      481 cacgccccgt caggtagtcg gcgaaggcct tcgcgccgcg ctgctcgacc ttcgggaact
      541 tcgccgggtc gagcagcgtg acgtgataga cgttcaggag cgcgggacca cgctcgacga
      601 ggaccgcgag gcccggcagc ttgtcgcggt tcgccagcca cgtgccgcgg tcggagagcg
      661 tgtacgccgt acgcgcgacc gcgagctgga ggttctggag ctgtccggtc ccggactcga
      721 ggtaccacgg ccggcccttc ggatcgcggc ccgcgtcgcg ccagagcgcg agctccttca
      781 cgttcgtccc cgagcggtcg ccgcgcgaga cgaatgatgc ccccgcgtcg tagatgcgac
      841 ggagcgcgtc ggtgacggca gcgcccttga ctcgcgctgg atcgctcgcg ggtccgagga
      901 tgacgaagtc gttgtgcatg accagccgcc gctcgctcgc gttgccggac ctcacgaacg
      961 cttcctcgtc cggcggcgag tgcgtgagga tgacgtcgag atcgccccgc gaagcctgcg
     1021 tcaggatctg cccgctgcca ccgacgacga gcttcacctt ccagccgctg cgccgctcga
     1081 agtccggcac gagcacgtcg agcaacccgg tgtcctgcgt cgtcgtcgtc gtgccgacga
     1141 gcacgctccc ctgcggctgt ggcgtcggcg acgaggccgg cgcctcggtg cacgcggacg
     1201 tgcccatcag gagcgcgaac agcacgagcg cgcgtgccgc gggtgacagg gcgaagcggg
     1261 tctctcgcgc cgcgggagtg gaggggcccc gcgcgcggcg gtacgccggc agagcggccc
     1321 acgcggggag gccaacccgc aagcgagaga ccccgagccc tggcaggacc cgcggcacgc
     1381 gcgcgcccgc cgtcattcac ggacctcgat cggcacgtcg cggaagcgcg cgtgcgcggc
     1441 gccgtggccc acgcgcatga gctgcagcgg ctcgcccttg gcgcacgaca ggaacccgtg
     1501 cagctcccag tcgtcggcga cgccgtcgca gcggccccag aactcgggcg tcagcgactg
     1561 gaaggacacg ccgcgtacgt aaccctgcag ctcgccgttg cggatgacgc gtgcggcctg
     1621 cgtcgagaag tggaagttct ggcgcttgtc gtcgagcgag tacgacgcgg gcgcctccag
     1681 cagcaggccg tcctccacgc ccttcaggag ctcggggtac gagcgcggtc cgggatcgag
     1741 cgagacgttg accatgcgga cgatcgggaa gtgctgccag ccctcggccc gcgcgctgcc
     1801 gatcgcgacg ggcatgtcga gcgccgcggc ggtctcgcgc gaggtgagat agccgacgaa
     1861 gatccccttg tcgacgagct tcgtccgcat cgccgggacg ccgtcgtcgt cccagccgaa
     1921 cgtgcccata ccgcccggga ccgtggcatc cgcggtcatc gagacgtgct cgctgccgta
     1981 gcggagcgtt ccccggtcct tcggccacat gaagctcgtg cccgcgaagg cgatctcgta
     2041 ctcgagcacg cggtcggcct cggtcggatg gccgcacgat tcgtgcacga ggagcgcgag
     2101 gaactccggc gcgaacacca gggtcgtcgg gcgttcggtc gcgagcggcg cgtccagcgt
     2161 cgcctcggcg tcgtcgcggt agcgcttcgc gcgcgcgacg agatccagcg cttcgacgaa
     2221 ctcccagccc gcctgccgcg tgagccgcga gtcgtagcgc tgcgcgggct tcgcgccggc
     2281 cttcaacgcg gtgaccgcga cacccgcgcc ggattccacg atctcctgcg acacgtcggt
     2341 gccctcgctc gtgacgagcc gcttgcgcgt gcggtacgcg gcgatcgacg agcgccgcga
     2401 tctcgcgttc gggccgcgca gcgcctcgtc cgccgcctgc agcagcgcga tgcgctctcg
     2461 catcggcacg gcgaacgggt cgcgccggat cgtgctgcgg tattcgccgt gctgcggcgc
     2521 caccggcgcc agcgtgatgg accgggatcg cagcgacgcg gacgcctcgg cacgacggaa
     2581 cgcctcgtcg acgagctcct tcatggcggc ctcgcccgtg cggtcggtcg cggcgaagcc
     2641 ccacgcaccg ttgtggatca cccggaagcc cacgccacga tcggaggtcc gcgtcaccga
     2701 ctcgacctgg ccgtcgcgca ggctcaccga ctcgtgttcg cggtcgacgg agcgcgcgtc
     2761 cgcgtacgtg gcgccgcgcg acgcggcgag atggacaagg tcagggaggg aggtctccag
     2821 ggagcgcatc cgcacgatgg agagtaggtg tacagtgcgc cgccatgctg gcaagcaggc
     2881 tcctcgcacc gctcgcggtc ggcgtcatgt gcgggatctg cgcgggcgcg ctcgccgggc
     2941 tctgggcgcg gatcgcgatg cgcctggtcg cgatcggggt cgccgatggc gtcggcatca
     3001 cgcccgaatt caccatcgct ggcacgctcg ccatcgtcat cgtcggcatc gtcgtcggcg
     3061 cgccggcggg cctcgtctac ggtcttgtcg cggatcggct gccgggacca gggcggtggc
     3121 gcggcctgct gtacgcggcg gtgctcctcg tgctcgtcgg tccgttcttc ttccgtatcg
     3181 aggagttctt cagcaccggg cgcgtactgc tcttcgtgcc gcccttcctt ctgtacggcc
     3241 tcgtcctcgg tctcgccctc gtgcccatgc gccggaccgt cacgcgcatg cccctcgggg
     3301 cgcaggccgc gctcgcggtc accggtctcg gcgcgctcgg gctgctcgcg ttcggcgtgg
     3361 ccggggcgat cttcggcgtg cccacgggct tcgtgatgta agcgtccggg tcagcggacg
     3421 acgatcttgc ccttcatccc cgcttcgtag tggccctgca cgcagcacgc gaactcgtat
     3481 tcgccggtct cgggcgggac caggacgttc agcgacgcgt accagtccgc gctcacacga
     3541 acaccctcgc cggtgtgcgc ttcgccgggg tgcgtgtggt ccggcacgtt cgtcgaggcc
     3601 ttcgcgatcc agtcctccgc gtagcccttg cccggcgtgg gctggcgccc ggccatgaat
     3661 tcgtgctcgg tcttgccgac gttct
//
LOCUS       DASTZR010000156         2058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_897328, whole genome shotgun sequence.
ACCESSION   DASTZR010000156 DASTZR010000000
VERSION     DASTZR010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897328"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..111)
                     /locus_tag="VFV20_03510"
     tRNA            complement(40..111)
                     /locus_tag="VFV20_03510"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(77..79),aa:Met,seq:cat)
     gene            complement(144..521)
                     /locus_tag="VFV20_03515"
     CDS             complement(144..521)
                     /locus_tag="VFV20_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287445.1"
                     /translation="MSHHKRRRQRPSLRPLVAIGLLLGGLVLASAFAGVTIQENALAR
                     EIGALNAQISVEQGKQASLERSAAEKKTSDYVVDKAKDLGFVWPWEALIAVQREADAR
                     AQLPESVRAPRIARWIALFVGTR"
     gene            complement(544..1356)
                     /locus_tag="VFV20_03520"
     CDS             complement(544..1356)
                     /locus_tag="VFV20_03520"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU5287446.1"
                     /translation="MQGELGAVRDAIVTARCRVGVLVRSADGHTILEHDADVAFPAAS
                     VIKVPLVMTLYGDAAEGRLDLRERVPAGARADGTGILRDLRDVDMLTLRDLAALTIGL
                     SDNTATNRLIERVGMARVAARLAEWGCQTTALRRAMFDFEAAKRGLENVATAREMVEL
                     LARLVDGRLVDRTTSDAVLALMERCDDDRKLRRYMPQNAKVANKSGTLDASRNDVAVF
                     RGPRATVLAAAFTSEVKDTLAAEHLLGLIGRAAADAAGIVLSPLPFAAGSGG"
     gene            1387..>2058
                     /locus_tag="VFV20_03525"
     CDS             1387..>2058
                     /locus_tag="VFV20_03525"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU5287447.1"
                     /translation="MIASRALARAAVLLASGLLVSCGLFSTQPSPSPSPSASPTRSRP
                     RFELSTYQYALQTRGKIRVAVRDGNTFAPLSSGSVQGKVEGFEADLARELARAIWGTS
                     DDPDSHIQWISVDNSTAVAALTSNQADVTFAGLLTGDDANRKVIDLSDSYLRTGPRLL
                     VKKANTTIKEISDVASGEQTVCAAKQGRPAQELRKITNNGAKVLELETVDFCMQALST
                     GAADAV"
BASE COUNT          324 a          758 c          676 g          300 t
ORIGIN      
        1 acctaacaaa aagcccggga tcattcccgg gccagactgt tgcgggggaa ggattcgaac
       61 ctccggcctc agggttatga gccctgcgag ctaccactgc tccaccccgc gatgtcagtc
      121 tagtctaccg cacttcatca cgctcatctc gtcccgacga acaacgcgat ccatcgcgcg
      181 atgcgcggcg ctcgcacgct ctccggcagc tgcgcgcgcg cgtccgcctc gcgctgcacc
      241 gcgatgagcg cctcccacgg ccatacgaac cccagatcct tcgccttgtc caccacgtaa
      301 tccgacgtct tcttctcggc ggcggatcgc tcgagcgacg cctgcttccc ctgctcgacc
      361 gagatctgcg cgttcagcgc gccgatctcg cgcgcgagcg cgttctcctg gatcgtcaca
      421 ccggcgaacg ccgacgcgag cacgagcccg ccgaggagca gtccgatggc gacgagcggc
      481 cgcagcgacg gccgctgccg gcgtctcttg tgatggctca tcgtcgcgtc cacatcccgc
      541 ccatcatccc ccactccccg ccgcgaacgg aagtggggac aacacgatcc cggccgcatc
      601 ggcggcggcc cggccgatca gcccgagcag atgctccgcg gcgagcgtgt ccttcacctc
      661 ggaggtgaac gcggccgcga gcaccgtggc gcgcggcccg cggaacacgg cgacgtcatt
      721 ccgcgacgcg tcgagcgtgc cgctcttgtt cgcgaccttc gcgttctggg gcatgtaccg
      781 gcgcagcttc cgatcgtcgt cacagcgctc catcagcgcg aggaccgcgt cggacgtggt
      841 gcggtcgacc agacggccgt cgaccagacg cgccaggagc tcgaccatct cgcgagcggt
      901 cgcgacgttc tcgagacctc gcttcgccgc ctcgaagtcg aacatcgcgc gacgcagcgc
      961 ggtggtctgg cagccccact cggcgaggcg ggccgccacg cgcgccattc ccacgcgctc
     1021 gatgaggcga ttcgtcgcgg tgttgtccga gagtccgatc gtgagtgcgg ccaggtcgcg
     1081 cagcgtgagc atgtcgacgt cgcgcagatc gcggaggatc ccggtgccgt cggcgcgagc
     1141 gcccgcgggt acgcgctcgc gcaggtcgag gcgtccctcc gcggcatcgc catagagcgt
     1201 catcacgagc ggcaccttga tgacggaagc cgcggggaac gcgacgtcgg catcgtgctc
     1261 gaggatcgtg tgcccgtcgg ccgaccgcac gagcacgccg acacggcatc gggcggtcac
     1321 gatggcatcg cggacggcgc cgagctctcc ctgcacgggc cgaggctagc gcctaggctt
     1381 ccgctcgtga tcgcctcgcg cgcgctggct cgcgccgcgg tccttctcgc gtcgggcctg
     1441 ctggtgtcgt gcggcctgtt cagcacccag ccgtcgccgt cgccgagtcc gtcggcctcg
     1501 ccgacgcggt cacgcccacg cttcgagctg tccacctacc agtacgcgct gcagacgcga
     1561 ggcaagatcc gcgtcgcggt gcgggacggc aacacgttcg cgcccctcag ctccggctcg
     1621 gtccagggaa aggtggaggg cttcgaggcg gacctcgccc gcgagctggc gcgggccatc
     1681 tggggcacga gcgacgatcc cgacagccac atccaatgga tctccgtcga caactcgacg
     1741 gcggtcgcgg ccctcacgag caatcaggcc gacgtcacgt tcgccggcct gctcaccggc
     1801 gacgacgcca acaggaaggt catcgatctg agcgacagct acctgcgcac ggggccgcgg
     1861 ctcctggtga agaaggcgaa caccacgatc aaggagatct ccgacgtcgc gagcggtgag
     1921 cagaccgtct gcgcggcgaa gcagggccgg cccgcccagg agctgcgaaa gatcaccaac
     1981 aacggcgcga aggtgctcga gctcgagacc gtcgacttct gcatgcaggc cctgtcgacc
     2041 ggcgccgcgg acgcggtc
//
LOCUS       DASTZR010000157         2638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_505578, whole genome shotgun sequence.
ACCESSION   DASTZR010000157 DASTZR010000000
VERSION     DASTZR010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505578"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..922
                     /locus_tag="VFV20_03530"
     CDS             56..922
                     /locus_tag="VFV20_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007617144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEU5287448.1"
                     /translation="MQLAAAPISWGVCEVPGWGFQLPPDRVLGDMLRLGLRDIEAGPP
                     GFLPDDDAVARALVTRMGLRVVAAFVTAVLHRPDVLEAELRAVDAQARRIAFLGGRVL
                     VLAAATGRDGYDAPYALAETEWRALADALPRVAALAARHGLTVALHPHVGTVIESAPA
                     IERVLRDSDVALCLDTGHVFVGGGDPVAIAREHASRVAHVHLKDADAALAAAARSRQV
                     PYGQAVARGLFRPLGDGHARIAEVLEALRDGGYAGYAVLEQDIALREPPSEANDPARD
                     VARSRDFVRPYV"
     gene            915..1856
                     /gene="iolC"
                     /locus_tag="VFV20_03535"
     CDS             915..1856
                     /gene="iolC"
                     /locus_tag="VFV20_03535"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014689888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HEU5287449.1"
                     /translation="MSRYDVLTIGRVSVDLYPEQTGVPLAQVRSFAKSLGGSPTNVAV
                     GAARLGRRTAAITKVGADGFGTYVRSALAGFGVDASFVGTHPTLRTPLVFCEIYPPDR
                     FPLLFYREPQAPDMTIGMDDLPMDAIRDAAIFWTTGTGLSAEPSRRATLAAMDAARGT
                     VIHDLDHRPALWASLEEARRWAGEAIRRAKVIIGNRDEFAMATGTSDPAHAASALLTA
                     GAELVIVKRGMEGASAFTMERRVHVSGQRVEVVCGLGAGDAFGGALAHGLLSGWELSR
                     TLAFANAAGAIVASRLACADAMPTAAEVEALLAGSKP"
     gene            1853..>2638
                     /locus_tag="VFV20_03540"
     CDS             1853..>2638
                     /locus_tag="VFV20_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEU5287450.1"
                     /translation="MSTVRLTMAQALIRFLAAQYVERDGVEERFFAGVFGIFGHGNVA
                     GIGQALFEHRDLLPFYQARNEQAMVHIASGYARMRDRLGTFACTSSIGPGATNMITGA
                     ALATIDRLPVLLLPGDVFASRRPDPVLQQLEVPSRGEVSVNDAFVPVSRYFDRITRPE
                     QIVPAALAAMRVLTSPADTGAVTLALPQDVQTEAFDVPESFLAKRVWHIGRPGPDTGA
                     FARLADLIRSSHRPLIVAGGGVVYARATAALARFAEASGIPVAE"
BASE COUNT          324 a          961 c          968 g          385 t
ORIGIN      
        1 atcctcatcg cgaccctccc cgctacctct cgccgctgga cgctagcgta cggtcatgca
       61 gcttgccgcg gcgcccatct cgtggggcgt gtgcgaggta ccgggctggg gatttcagct
      121 gccgccggac cgcgtgctcg gcgacatgct gcgcctcggt ctgcgcgaca tcgaggccgg
      181 gccaccgggc ttcctccccg acgacgacgc cgtggcacgc gcgctcgtca cgcgcatggg
      241 ccttcgcgtc gtcgcggcgt tcgtcacggc ggtgctccat cgaccggacg tgctggaggc
      301 cgagctgcgc gcggtggacg cccaggcgcg gcgcatcgcg ttcctcggcg gccgcgtgct
      361 ggtgctcgcg gccgcaacgg gacgtgatgg ctacgacgcc ccgtacgccc tcgcggagac
      421 ggagtggcgc gcgctcgcgg acgctctgcc gcgcgtggcg gcgctcgcgg cgaggcacgg
      481 cctcacggtc gcgctgcacc cgcacgtcgg cacggtcatc gagagcgctc ccgcgatcga
      541 gcgcgtgctg cgcgacagcg acgtcgcgct ctgcctcgac accggacacg tcttcgtggg
      601 tgggggcgac ccggtcgcga tcgcgcgcga gcacgcctcg cgcgtcgcgc acgtccacct
      661 caaagatgcc gacgcggcgc tcgcggcggc ggcgaggtcg cggcaggtgc cgtacggcca
      721 ggccgtcgcg cgcgggctct tccgtccgct cggtgacggc cacgcgcgca tcgccgaggt
      781 gctcgaggcg ctccgcgacg gcgggtacgc gggctatgcc gtgctcgagc aggacatcgc
      841 gctgcgcgag ccgccgagcg aggcgaacga ccccgcgcgc gacgtggcgc ggagccgtga
      901 cttcgtgagg ccgtatgtct aggtacgacg tgctcaccat cggccgtgtg tcggtcgatc
      961 tctaccccga gcaaacaggc gttccgctcg cccaggtccg cagcttcgcg aagtcgctgg
     1021 gcgggagccc cacgaacgtg gcggtcggcg cggcccgcct gggccggcgt acggcggcga
     1081 tcaccaaggt cggcgccgat ggcttcggca cgtacgtgcg cagcgctctg gccggcttcg
     1141 gcgtcgacgc gtccttcgtc ggcacgcatc cgacgctgcg gacaccgctc gtcttctgcg
     1201 agatctaccc gccggaccgc ttcccgctcc tgttctaccg ggaaccgcag gcgccggaca
     1261 tgaccatcgg gatggacgac ctgccgatgg acgccatccg cgacgccgcg atcttctgga
     1321 ccacgggcac cggcctgtcc gcggagccga gccgccgcgc gacgctcgcc gcgatggacg
     1381 cggcgagggg aaccgtgatc cacgacctcg accaccgtcc ggcgctctgg gcgtcgctcg
     1441 aggaggcgcg ccgctgggcc ggcgaagcca tacgccgcgc gaaggtcatc attggcaatc
     1501 gcgatgagtt cgcgatggcc acgggcacga gcgatcccgc gcacgccgcg tcggcgctgc
     1561 tcacggccgg ggccgagctg gtgatcgtga agcgcgggat ggagggcgcg tcggcgttca
     1621 cgatggagcg gcgcgtgcac gtctccgggc agcgcgtcga ggtggtgtgc ggcctcggcg
     1681 cgggcgacgc cttcggcggc gcgctggcgc acgggctcct ctccggatgg gagctgagcc
     1741 gcacgctcgc gttcgccaac gccgcgggcg cgatcgtcgc gtcgcgcctc gcctgcgcgg
     1801 acgccatgcc caccgccgcc gaggtcgagg cgctgctcgc gggatcgaag ccatgagcac
     1861 cgtgcgtctc accatggcgc aggccctcat ccgcttcctc gcggcgcagt acgtcgagcg
     1921 cgacggcgtc gaggagcgct tcttcgcggg cgtgttcggg atcttcggtc acggcaacgt
     1981 cgcgggcatc ggacaggcgc tcttcgagca ccgggacctt ctcccgttct accaggctcg
     2041 caacgagcag gcgatggtgc acatcgcgtc gggatacgcg cggatgcgcg accggctcgg
     2101 gaccttcgcg tgcacgagct ccatcggtcc gggcgcgacc aacatgatca ccggcgccgc
     2161 gctggcgacg atcgatcggc tgccggtgct gctgcttccg ggtgacgtct tcgcgtcgcg
     2221 gcggcctgac ccggtgctcc agcagctcga ggtgccgtcc cgcggtgagg tgagcgtgaa
     2281 cgacgcgttc gtgccggtct cgcgctactt cgaccggatc acgcgccccg agcagatcgt
     2341 cccggcggcg ctcgcggcga tgcgcgtcct caccagtccc gcggacacgg gcgccgtcac
     2401 gctcgcgctg ccgcaggacg tgcagaccga ggcattcgac gttccggaga gcttcctcgc
     2461 gaagcgcgtg tggcacatcg gccgtcccgg acccgacacg ggcgcgttcg cgcgtctggc
     2521 ggacctgatc cgctcgtcgc accggccgct catcgtcgcg ggcggcgggg tcgtgtacgc
     2581 gagagcgacc gccgcgctgg cgcgtttcgc cgaggcgagc gggatcccgg tggccgag
//
LOCUS       DASTZR010000158         2980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_636006, whole genome shotgun sequence.
ACCESSION   DASTZR010000158 DASTZR010000000
VERSION     DASTZR010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636006"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VFV20_03545"
     CDS             <1..530
                     /locus_tag="VFV20_03545"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEU5287451.1"
                     /translation="GVSANTVTVLGLLLTIAGATLLALGNPVAALAVLLVGAIADTLD
                     GQIARAAGGGTRLGAFLDSTFDRLSDAAFAVAAIALGASRAEPLLFWSGVVALVAGSL
                     VPYIRAKAESLGLSASVGLAPREARLALLVLGVAAWAATGSIQLFAAAVVVVAFLSTV
                     TTIQRIAHVARSLNT"
     gene            543..1682
                     /locus_tag="VFV20_03550"
     CDS             543..1682
                     /locus_tag="VFV20_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEU5287452.1"
                     /translation="MPTTKRKKATRARGARRKIRVAIIGVGNCASSLVQGVYYYRNAK
                     PGDQIPGIMHVDLGGYHLSDLEFVAAFDVDKNKVGRDLADAIYAKPNNTYRFADVPKT
                     GVKVSRGMTLDGIGKYLWKVVTKAPGETDDIVGILRDRKVDVVVSYLPVGSEDATRWY
                     VEQILQAGCAMVNCIPVFIGREPRWQKRFAAAKLPIVGDDIKSQVGATITHRVLTRLF
                     MDRGVRLDRTYQLNFGGNTDFLNMLERERLESKKISKTNAVTSMLDYPLPADNVHVGP
                     SDYVPWLLDRKWCYIRMEGTTFGDVPLNAEVKLEVWDSPNSAGVVIDAIRCCKLGLDR
                     GLGGTLVAPSAYFMKSPPKQIPDDKAREGVEAFIRGEDSRVLTGK"
     gene            1683..2591
                     /locus_tag="VFV20_03555"
     CDS             1683..2591
                     /locus_tag="VFV20_03555"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU5287453.1"
                     /translation="MRSHLVEGVLYWGWRSGALLVQRLPARFVYACAVLGGEIAYLGW
                     RTKREITKRNFAIVLGRSSTDPEVARVSRRSFRNFAKYLTEIMRFPSLRAHDLERMVV
                     IDPRSLGHLRAAREHGKGLIFVSAHFGNFELGGARIAQEIPLTVVADELENQRLMDFL
                     VANREHKNVNILAPEGSAKHVLAALKRNEMVGFMMDLGPRAHELANVQADFFGSATTF
                     PTIAAALARVSGAPIVVAAVKRERDNTFRGVALPPIFVERTKQAAHDLEHTTQAIVRA
                     LEGLVRPDPDQWYIFRPMWPTAASPS"
     gene            2594..>2980
                     /locus_tag="VFV20_03560"
     CDS             2594..>2980
                     /locus_tag="VFV20_03560"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287454.1"
                     /translation="MPAAGLVHAAVRALPSLPSALDAPLAELVGSLAYAAQPRARAAV
                     RANLATVAAGRRMAPRRVFVEQARNYLEIFRIPRMRPERLRAMVEKPGWEHFLAAHAR
                     GRGVIVASAHLGPVSLVGQVLSAHGYE"
BASE COUNT          453 a         1042 c         1029 g          456 t
ORIGIN      
        1 ggggcgtgag cgcgaacacc gtcacggtgc tcggcctgct gctcacgatc gccggtgcca
       61 ctctgctcgc gctcggcaac cccgtggcgg cgctcgcggt cctgctggtg ggcgccatcg
      121 cggacacgct cgacgggcag atcgcgcgag ccgccggcgg gggcaccagg ctcggcgcgt
      181 tcctcgactc gacgttcgac cggctctccg acgccgcctt cgcggtcgcc gcgatcgcgc
      241 tgggcgcgtc gcgcgcggag ccgctgctct tctggtccgg cgtcgtcgcg ctcgtcgcgg
      301 gttccctcgt cccgtacatc cgcgcgaagg cggagtccct ggggctcagc gcgagcgtgg
      361 ggctcgcccc gcgtgaggcc cgcctcgcgc tcctcgtcct cggcgtcgcg gcctgggccg
      421 cgaccggcag catccagctc ttcgcggcgg cggtcgtcgt cgtcgcgttc ctttccaccg
      481 tcaccaccat ccagcggatc gcgcacgtcg cgcggtcgct gaatacataa agggggatac
      541 atatgccaac gaccaaacgc aagaaggcga cgagggcgcg cggcgcgcga cgcaagatcc
      601 gcgtggccat catcggcgtc ggcaactgcg ccagttcgct cgtccagggc gtgtactact
      661 accggaacgc caagcccggc gaccagatcc cggggatcat gcacgtggac ctcggcggct
      721 accacctctc ggatctggag ttcgtcgccg cgttcgacgt ggacaagaac aaggtcggga
      781 gggacctggc ggacgcgatc tacgcgaagc cgaacaacac ctaccgcttc gccgacgtgc
      841 cgaagaccgg cgtgaaggtg agccgcggga tgaccctcga cggcatcggc aagtacctct
      901 ggaaggtcgt caccaaggcg ccgggcgaga cagacgacat cgtcggcatc ctgagggatc
      961 ggaaggtgga cgtcgtggtg tcgtacctcc cggtcggcag cgaggacgcc acgcgctggt
     1021 acgtcgaaca gatcctccag gcgggctgcg cgatggtgaa ctgcatcccg gtgttcatcg
     1081 ggcgcgagcc gcgctggcag aagcgcttcg cggcggcgaa gcttccgatc gtcggcgacg
     1141 acatcaagtc gcaggtgggc gccacgatca cgcatcgcgt gctcacgcgc ctgttcatgg
     1201 accgcggcgt gcgtctcgac cgcacgtatc agctgaactt cggtggcaac accgacttcc
     1261 tgaacatgct cgagcgggag cggctcgagt cgaagaagat cagcaagacg aacgcggtca
     1321 cgagcatgct cgactaccct cttccggcgg acaacgtcca cgtggggccg tccgactacg
     1381 tgccgtggct gctggaccga aagtggtgct acatccgcat ggagggcacg acgttcggcg
     1441 acgtgccact caacgcggag gtgaagctcg aggtgtggga ctcgccgaac agcgcgggcg
     1501 tggtcatcga cgccatccgc tgctgcaagc tgggcctgga ccgcggcctc ggcggtacgc
     1561 tcgtcgcgcc gtcggcgtac ttcatgaagt cgccgccgaa gcagatcccg gacgacaagg
     1621 cccgcgaggg cgtcgaggcg ttcatccgcg gcgaggacag tcgcgtgctc acagggaaat
     1681 aggtgcggtc acatctcgta gaaggcgtcc tctactgggg atggcggtcg ggggcgctgc
     1741 tcgtgcagcg gctccccgcc cggttcgtct acgcgtgcgc cgtcctgggc ggcgagatcg
     1801 cctacctcgg ctggcggacc aagcgtgaaa tcacgaagcg gaacttcgcg atcgtcctgg
     1861 gacgctcctc cacggatccg gaggtcgcgc gcgtgtcgcg ccgaagcttc cgcaacttcg
     1921 cgaagtacct caccgagatc atgcggttcc cgtcgctgcg ggcgcacgac ctcgagcgca
     1981 tggtcgtcat cgatccgcgc tcgctcggtc acctgcgcgc ggcccgcgag cacggtaagg
     2041 gcctcatctt cgtatcggcg cacttcggca acttcgagct cggcggggcg cggatcgcgc
     2101 aggagatccc gctcaccgtg gtggccgacg agctcgagaa ccagcggctc atggacttcc
     2161 tcgtcgcgaa ccgcgagcac aagaacgtca acatcctcgc cccggagggg tcggccaagc
     2221 acgtgctcgc cgcgctcaaa cggaacgaga tggtgggatt catgatggat ctcgggcccc
     2281 gggcgcacga gctcgccaac gtccaggccg acttcttcgg ctccgccacg accttcccga
     2341 ccatcgccgc cgccctcgcg cgggtgtcgg gtgcgccgat cgtcgtggcg gcggtgaagc
     2401 gcgagcggga caacaccttc cggggtgtgg ccctgccgcc gatcttcgtc gagcgcacca
     2461 agcaggcggc gcacgatctc gagcacacga cgcaggcgat cgtgcgggcg ctcgagggtc
     2521 tggtccggcc ggatcccgac cagtggtaca tcttcagacc gatgtggccg acagccgcat
     2581 cgccgagcta gccatgccgg ccgccggcct cgtccacgcg gccgtgcgcg cgctgccctc
     2641 actgccgtcc gccctcgacg ctccgctcgc cgagctcgtc ggatcgctcg cgtatgcggc
     2701 acagccgcga gcgcgggcgg cggtccgcgc gaatctcgcg acggtcgcgg cgggccgccg
     2761 gatggcgccg cgccgcgtct tcgtcgagca ggcgcggaac tacctcgaga tcttccgcat
     2821 cccgcgcatg cgccccgagc ggctgcgcgc gatggtcgag aagcccggct gggagcactt
     2881 cctcgccgcg cacgcgcgtg gccggggagt catcgtcgcg tccgcgcacc tgggccccgt
     2941 gtcgctcgtc gggcaggtcc tgtcggcgca tggctacgag
//
LOCUS       DASTZR010000159         3339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_714195, whole genome shotgun sequence.
ACCESSION   DASTZR010000159 DASTZR010000000
VERSION     DASTZR010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714195"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VFV20_03565"
     CDS             complement(<1..246)
                     /locus_tag="VFV20_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287455.1"
                     /translation="MARVPGDLILEPSERVVLATRPLFLWEPLAILEVLLFFGSLYLY
                     GRSGAAGGTDVRAPQLEYVSYILLALFVLLLVWIVVKW"
     gene            complement(256..1308)
                     /locus_tag="VFV20_03570"
     CDS             complement(256..1308)
                     /locus_tag="VFV20_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU5287456.1"
                     /translation="MGSTGYETRVAAIDDEIRSLLTQAEAALQPFYGMMLYHLGLDAE
                     HARGGKRLRPLLAALVFETLGGDSARVTALGAALELLHNFTLIHDDIEDQDPRRHHRP
                     TVWSVWGEPQAINAGDGMYAISRLAVQRLRERGFAAERILDLAALLDQTCVRVCEGQF
                     LDISFEARTDVTADRYRAMAARKTGALFAASARGAAVLATDERTVHEQLSRFGAEFGH
                     SFQARDDVQGIWATSDRTGKVEMNDLVKRKKTLPVVLAFEKASPKTRAVLDTLFAPAA
                     PLPAENVERIRQILDELSVRDLIEHEIAEHRERALQLLRGVPGLVPGQPLALLEQLVG
                     TATGATEAAAPAGTPS"
     gene            1344..2120
                     /gene="recO"
                     /locus_tag="VFV20_03575"
     CDS             1344..2120
                     /gene="recO"
                     /locus_tag="VFV20_03575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU5287457.1"
                     /translation="MASRTYRAEAIVLRTVDLGEADRILVLFTRHFGKVHVVAKGIRR
                     ATSRMAGHAEPLTHATYQLARGRELDVLTGAEARALYPTLREDLGSIAAGWYCAELID
                     RFSVERAPGAPLFDLLETALRHLDAGYAATLVCRWFDLHLLDRSGFRPELAVCVSCRT
                     ALEERENAWNAAAGGALCPACARRVPGLSLSVRALKSLRYLLVSDFASAARLRVDAAL
                     AGELERHMRLFVQHVIDRDITAARLIDEIRALPIRPATAS"
     gene            2123..>3339
                     /locus_tag="VFV20_03580"
     CDS             2123..>3339
                     /locus_tag="VFV20_03580"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEU5287458.1"
                     /translation="MTERTMPEQVADKLTSLAKRRGLVFPSSEIYGGAGATWDYGPLG
                     VELKNNVKRAWWKAMVQLRDDVVGLDGAILMHPKVWEASGHVENFTDPLVECANCRKR
                     FRLDELPKGEELASEWRGGKVDMTAVACPACGMRKLGTPRRFNLMFKTFVGPVEDSAS
                     VAWLRPETAQSIFVDFESVQQSSRKKLPFGIAQIGKAFRNEITPGNFIFRSREFEQME
                     MQYFCRPDDAPRLFEEWKQHRMEWHRAYGMPGEFLRFRDHAPEERAHYAAAATDIQFR
                     FPFGWQEMEGIHNRTDYDLRRHGEYSGKDLRYFDEGTNERLIPYVIETSVGADRATYA
                     FLCAAYDEEPDKEGVRVVLRLHRDLAPYKVAVLPLSKNEKLTPLAREVSAQIRPHFMT
                     TYDETQAIGRRYRR"
BASE COUNT          501 a         1155 c         1189 g          494 t
ORIGIN      
        1 ccacttgacc acgatccaga caaggagaag cacgaacagc gcgagcagga tgtaggacac
       61 gtattccagc tgcggggccc gtacgtcggt cccgccggcc gctccgctcc gtccgtagag
      121 gtagagcgag ccgaagaaga ggagcacctc gaggatcgcg agcggctccc agaggaagag
      181 cggacgcgtc gcgaggacga cgcgctcgct cggttcgagg atgagatcgc ccgggacccg
      241 tgccatgccg agatgctagg acggcgtgcc ggcaggcgcc gcggcctccg tcgcgccggt
      301 ggccgtgccg acgagctgct cgagcagcgc gagcggctgg cccgggacga gcccggggac
      361 gccacgcagg agctggaggg cgcgctcgcg atgctcggcg atctcatgct cgatgaggtc
      421 ccgcaccgac agctcgtcga ggatctgccg gatccgctcg acgttctcgg cgggcagcgg
      481 cgcggccggc gcgaagagcg tgtcgaggac ggcgcgcgtc ttcggcgacg ccttctcgaa
      541 ggcgaggacg acggggagcg tcttcttgcg cttcacgagg tcgttcatct cgaccttccc
      601 ggtgcggtcg ctcgtggccc agatgccctg gacgtcgtcg cgtgcctgga acgaatgccc
      661 gaactcggcg ccgaagcgcg acagctgctc gtgcaccgtc ctctcgtccg tcgccagcac
      721 ggcggcgccg cgcgcggagg cggcgaagag cgcgccggtc ttgcgcgcgg ccatggcacg
      781 gtagcggtcg gccgtgacat cggtccgcgc ttcgaagctg atgtcgagga actggccctc
      841 gcagacgcgc acgcaggtct ggtcgaggag cgcggcgagg tcgaggatcc gctcggccgc
      901 gaagccgcgc tcgcgcaggc gctgcacggc caggcgggag atggcgtaca tgccgtcgcc
      961 ggcgttgatg gcctgcggct cgccccagac cgaccacacc gtcggacggt ggtggcgtcg
     1021 cgggtcctga tcctcgatgt cgtcgtggat cagcgtgaag ttgtggagca gctccagggc
     1081 cgcgccgagc gcggtgacgc gagcgctgtc gccgccgagg gtctcgaaga ccagcgccgc
     1141 gaggaggggc cggagacgct tgccgccgcg ggcgtgctcg gcgtcgaggc cgaggtggta
     1201 gagcatcatc ccgtagaacg gctggagcgc cgcctcggcc tgcgtcagga gcgagcggat
     1261 ctcgtcgtcg atcgccgcga ctcgtgtttc gtacccggtc gagcccaccg tcttccgatg
     1321 atatgcggct accattcctg ttgatggcgt cgcgcacgta tcgtgccgag gcgatcgtcc
     1381 tgcggacggt cgaccttggc gaggcggacc gcatcctcgt gctcttcacc cgtcatttcg
     1441 gcaaggtcca cgtcgtcgcc aagggcatcc ggcgcgcgac gagccgtatg gcggggcacg
     1501 cggagccgct gacgcacgcc acgtatcagc tcgcgcgtgg ccgtgagctt gacgtgctca
     1561 ccggcgcgga ggcgcgggcg ctgtatccga cgctgcgcga agacctcggc agcatcgccg
     1621 ccggctggta ctgcgcggag ctgatcgacc gcttcagcgt cgaacgcgcg cccggtgcgc
     1681 ccctcttcga cctgctggag accgcgctcc ggcacctcga cgccggctat gccgcgacgc
     1741 tggtctgccg gtggttcgac ctgcatctcc tcgaccgctc cggcttccgc cccgagctcg
     1801 cggtgtgcgt gtcgtgccgg acggcgctcg aggagcgcga gaacgcctgg aacgccgcgg
     1861 ccggcggcgc cctttgcccg gcgtgcgcgc gccgcgtgcc cggcctgtcc ctctccgtgc
     1921 gcgcgctgaa gtcgctgcgc tacctgctgg tgtccgactt cgcgagcgcc gcgcggctcc
     1981 gcgtggacgc cgccctcgcc ggcgagctcg agcggcacat gcggctcttc gtgcagcacg
     2041 tgatcgaccg cgacatcacc gccgcgcgtc tcatcgacga gatccgcgcg ctccccatcc
     2101 gcccggcgac cgcctcctga cgatgaccga gcgcaccatg ccggagcagg tcgccgacaa
     2161 gctcacctcg ctcgcgaaac ggcgcggcct ggtgttcccg tcgagcgaga tctacggcgg
     2221 cgccggcgcc acctgggact acggtccgct cggcgtggag ctgaagaaca acgtgaagcg
     2281 cgcctggtgg aaggcgatgg tccagttgcg cgacgacgtc gtcgggctgg acggcgcgat
     2341 cctcatgcat ccgaaggtgt gggaggcgtc gggccacgtc gagaacttca ccgacccgct
     2401 cgtcgagtgc gccaactgcc gcaagcgctt ccgcctcgac gagctgccga agggggagga
     2461 gctcgcgagc gagtggcgcg gcggcaaggt cgacatgacc gccgtcgcct gtcccgcgtg
     2521 cggcatgcgg aagctcggca cgccgcgccg cttcaacctc atgttcaaga cgttcgtcgg
     2581 cccggtcgag gactcggcgt cggtcgcgtg gctgcggccg gagacggcgc agagcatctt
     2641 cgtcgacttc gagagcgtgc agcagtcgtc gcgcaagaag ctgccgttcg ggatcgcgca
     2701 gatcggcaag gccttccgca acgagatcac gccgggaaac ttcatcttcc gctcgcgcga
     2761 gttcgagcag atggagatgc agtacttctg ccgtcccgac gacgctccgc gcctcttcga
     2821 ggaatggaag cagcaccgca tggagtggca ccgcgcgtac ggcatgcccg gcgaattcct
     2881 gcgcttccgc gaccacgcgc cggaggagcg cgcgcactac gccgcggccg ccacggacat
     2941 ccagttccgc ttcccgttcg ggtggcagga gatggagggc atccacaacc gcaccgacta
     3001 cgacctgcgc cgccacggcg agtactcggg caaggatctc cgctacttcg acgaggggac
     3061 gaacgaacgg ctcatcccgt acgtcatcga gacctcggtg ggcgcggacc gggcgacgta
     3121 cgcgttcctc tgcgccgcgt acgacgagga gcccgacaag gagggcgtgc gggtcgtgct
     3181 gcggctgcac cgggatctcg cgccgtacaa ggtggccgtc ctgccgctct cgaagaacga
     3241 gaagctcacg ccactggcgc gggaggtctc cgcgcagatc cgtccgcact tcatgaccac
     3301 gtatgacgag acgcaggcca tcggccgccg ctatcgccg
//
LOCUS       DASTZR010000160         2176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_114154, whole genome shotgun sequence.
ACCESSION   DASTZR010000160 DASTZR010000000
VERSION     DASTZR010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114154"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1602)
                     /locus_tag="VFV20_03585"
     CDS             complement(<1..1602)
                     /locus_tag="VFV20_03585"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU5287459.1"
                     /translation="MKTGERPTVTLTTPRAKVSLSAEKIVADYRLAVRSRAASELGRR
                     EVLVGRAPFGIFGDGKEIANLGMAHAFRPGDWRSGYYRDQTFHFAVGLATIDQYFAQL
                     YADPDPAREPFSSGRQMSNHYSSRTIDERGRWVDLLASGQTASDISPVAGQMPRSVGL
                     AWASKLYRNSPLLRGVASGFSRGGDEVCFATIGNAGAAEGIFWESVNAAGVLQIPLVV
                     AVWDDGYGISVPNEYQMTKSSVSAALRGLQRNGGPGYEIRTVKGWDYVGLIDTFAEVT
                     AIARREHVPALVHVVEVTQPQGHSTSGSHERYKPPERLRFEVEADPIKRMREWMIRED
                     IADAATLDEYERRDRADVEAIRERAYEAFQAPLRRERDAVAGLIEAAAAESEVDLGSL
                     VTELREETDVNRRLTHSLAFRALVALRETTGPARSELAAFLERSRAENTTRYTSHLLS
                     ETETSALRVPPVAPRYAADAETLDGRMVLLRCFDANLARDPRIVVLGEDVGILGDVNL
                     VYEGLQAKYGLDRIIDTGIREATIMG"
     gene            complement(1636..>2176)
                     /locus_tag="VFV20_03590"
     CDS             complement(1636..>2176)
                     /locus_tag="VFV20_03590"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5287460.1"
                     /translation="YMTAVTSPERSRSLTLVASGGLGTDIALFLRLATLPGAITLARI
                     FGRPGHGREVLRTCFFDPARIPATLYDEAERYGFASYPEFVRALRSGVTVRGVRPSLR
                     SAWLDRAERYRGPVLVVWGREDAVLPIHHLEDAKSVFPQAEVRIIERCGHLPMVEQTT
                     AFLDALLPFLARADTAVAA"
BASE COUNT          326 a          762 c          768 g          320 t
ORIGIN      
        1 gcccatgatg gtcgcctcgc ggatgcccgt gtcgatgatg cggtccaggc cgtatttcgc
       61 ctgcaggccc tcgtacacga ggttgacgtc gcccaggata ccgacgtcct cgcccagcac
      121 cacgatgcgc gggtcgcgcg ccaggttcgc gtcgaagcag cgcagcagca ccatgcgtcc
      181 atcgagcgtc tccgcatccg ccgcgtacct gggcgcgacg ggtgggacgc gcagcgcgga
      241 cgtctcggtc tcgctcagca ggtgcgaggt gtaccgcgtg gtgttctcgg cccgcgagcg
      301 ctcgaggaac gcggcgagct cggagcgtgc cggcccggtc gtttcccgca acgcgacgag
      361 cgcgcggaac gcgagcgaat gcgtgagccg ccggttgacg tcggtctcct cgcgcagctc
      421 cgtgaccagc gagccaaggt cgacctccga ctcggcggcc gccgcctcta tgagtcccgc
      481 gaccgcgtcg cgctcgcggc gcagcggcgc ctggaaggcc tcgtacgcgc gctcgcggat
      541 ggcctccaca tcggcgcggt cgcgacgctc gtactcatcg agcgtcgcgg cgtcggcgat
      601 gtcctcgcgg atcatccatt cacgcatgcg cttgatcggg tccgcctcga cctcgaagcg
      661 cagccgctcg ggcggcttgt agcgttcgtg gctgccgctg gtggagtgcc cctgcggctg
      721 cgtcacctcg accacgtgca cgagcgcggg cacgtgctcg cgccgggcga tcgcggtcac
      781 ttcggcgaac gtgtcgatga ggccgacgta gtcccacccc ttcacggtgc ggatctcgta
      841 gcccgggccg ccgttccgct gaaggccgcg cagcgcggcc gacaccgagc tcttcgtcat
      901 ctggtattcg ttcgggacgg agatgccgta gccgtcgtcc cagaccgcaa cgacgagcgg
      961 gatctgcagc acccccgcgg cgttgaccga ctcccagaag atcccctcgg ccgcgccggc
     1021 gttgccgatc gtcgcgaagc acacctcgtc gccgccccgc gagaagccgc tcgcgacgcc
     1081 gcgcaggagc ggcgagttgc ggtagagctt cgacgcccac gcgaggccga cggaccgcgg
     1141 catctggccc gcgactggag agatgtcgga cgccgtctga cccgaggcga gcagatcgac
     1201 ccagcgaccg cgctcgtcga tcgtgcgcga cgagtagtgg ttggacatct gccgtcccga
     1261 cgagaacggc tcgcgcgccg gatcgggatc cgcgtacagc tgcgcgaagt actggtcgat
     1321 cgtcgcgagg ccgaccgcga agtggaacgt ctgatcgcgg tagtagccgc tgcgccagtc
     1381 gccgggccgg aaggcgtgcg ccatgccgag gttcgcgatc tccttgccgt cgccgaagat
     1441 gccgaagggc gcgcgcccga cgagcacctc gcgccggccg agctcgctcg cggcccggct
     1501 gcggacggcg agccggtagt cggcgacgat cttctctgcg gagagactga ccttggcccg
     1561 cggcgtggtg agggtgaccg tggggcgttc gccggtcttc atgcgtacgt caatggtgcc
     1621 attcctgacg ctcggtcagg cggcgaccgc cgtgtcggcc cgcgccagga acggcaggag
     1681 ggcatcgagg aacgcggtcg tctgctccac catcggaagg tgtccgcatc gctcgatgat
     1741 gcgcacttcc gcctgtggga acaccgattt cgcgtcctcg aggtgatgga tcggcaggac
     1801 cgcatcctcg cggccccaca cgacgagcac cgggcccctg tagcgctcgg ccctatcgag
     1861 ccacgccgaa cgcagcgagg ggcgcacgcc gcgaacggtc acgccggaac gcagcgcgcg
     1921 gacgaactcc ggatatgagg cgaagccgta ccgctcggcc tcgtcataga gcgtcgctgg
     1981 gatgcgcgcc ggatcgaaga agcacgtccg cagcacctcg cggccgtggc ccggacgtcc
     2041 aaagatgcgt gcgagcgtga tcgcgccggg aagggtcgcg agacgaagga agagggcgat
     2101 gtccgttccc aggccgccgc tcgcgaccag cgtgagcgac cgcgagcgct ccggcgacgt
     2161 gaccgcggtc atgtac
//
LOCUS       DASTZR010000161         6863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_453672, whole genome shotgun sequence.
ACCESSION   DASTZR010000161 DASTZR010000000
VERSION     DASTZR010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6863
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453672"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VFV20_03595"
     CDS             <1..456
                     /locus_tag="VFV20_03595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEU5287461.1"
                     /translation="ARRTARRDEIVNALDLMAGVRDYLTDARRLGLRIGIASSSTRGW
                     VCGHLERLAVREGWDAIVCREDVQDAKPSPELYQKAVGLLGVAPQEALALEDSPNGIA
                     AAKAAGLRCVAVPNPLTADLDLRQADMRIASLAELSLGELIRRLDGSAV"
     gene            469..1545
                     /locus_tag="VFV20_03600"
     CDS             469..1545
                     /locus_tag="VFV20_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5287462.1"
                     /translation="MAAVIPAEEKLTSRLDPRARLGAVWRHALLRAVLKAVLTVLVAT
                     AITFTLIRLLPGNPIDLRIDELTRDGSMTYEQAREQVSGIYPVDLNAPIPLQFASYLW
                     NVLHGDLGTSFLATSVPVMAIILAVLPWTLFAVGTGLLLSFAVGILLGLLAAYHRGSA
                     FDQAVTTIGSIVSSVPNYLIAILIVLIFGIQLRLIPITQMRGALSPGVQPGLTLDFIG
                     DVLFHAALPITVFFLTNIGHWILSMRNATLAALEEDYVVAARARGLSESRITTAYVGR
                     NAALPLVTQLAISIGFIVGGAVLIEQVFLYQGVGLRLLRAVAQRDYPVMQGIVLLVTI
                     SVVVANLAADLLYSKLDPRIGRAS"
     gene            1542..2432
                     /locus_tag="VFV20_03605"
     CDS             1542..2432
                     /locus_tag="VFV20_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5287463.1"
                     /translation="MSAALRSVRLPSFVAELGALIAVIARRPAGLVGLIGVGGFLFLA
                     YVAPFFVPLHNAVDIANVYRTPSLDHPLGTDFQGRDVLNQIVYGGRDILSIAFLAALF
                     STAIAVTFGSLAATLGGRTDTIVLAVADIVLTVPHLIVLIVVAAIFRPEGFLVLAILL
                     ASLQWAGLLRQVRAQILSLKEREYVEAARSLDLGLFHVIFREMLPNMASYIAIHFIFA
                     MTGAVYAQVGLTVLGLVPLSGANWGVMLQLALQQGAIYFQDSFWYVLSPILAIALFQL
                     SLVALASGLEDVFNPRLRAG"
     gene            2432..3451
                     /locus_tag="VFV20_03610"
     CDS             2432..3451
                     /locus_tag="VFV20_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017570028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5287464.1"
                     /translation="MPTLLSVRDLAIEYRSRRGAVQAVRGVSLEVAEGETVSVIGESG
                     SGKTTLAVALIQLIPRSARVTRGEVLFHHDGRVDDVLAMPADELRHFRWSEAAMVFQS
                     ALNSFKPVIDIWSQFLDTARAHGMRDARAVRDRADELLDLVHLDPTRVLRAYPHELSG
                     GMKQRVLIALSLLLRPRLLILDEPTTALDILTQRAIIDLLRELREKLGFAMIFISHDL
                     SLAAELADRVLTMYAGRIVEEARVGDLFYAPRHPYTVGLLRAVPRVSGEVGTLSSIPG
                     TPPDLIHPPPGCAYHPRCPFAIERCRAEDPALVSVDTPAHTAACIRWQAVAESVGTRR
                     PAEAL"
     gene            3451..4479
                     /locus_tag="VFV20_03615"
     CDS             3451..4479
                     /locus_tag="VFV20_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017565337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5287465.1"
                     /translation="MTVRDVTAPGPHLIELDAVSRSFETPGGRITAVDRVSLALAAGE
                     AICLVGESGCGKTTTGRLLAGLLRPTGGRLLYEGKDVWEKKGADLAAFRRAVQLVHQD
                     PYASLNPVRTVYDTLSAPLFRHHKARGRREAIETVRELLRRVGLTPPEDFLPKYPHQL
                     SGGQRQRVAIARALSVDPRVVVADEAVSMVDVSIRITLLDLLLALRDEFGVSIVLITH
                     DLAVARYFARRGRIGVMYLGRVVELAPTEALVTDPAHPYAAALIGAIPEADPTLTRTK
                     ERVPLRGADVPSLLDVPPGCSFHPRCPLFEAGLCDVLVPRLLPIPDEREVACHVAVRE
                     RTGSVAGV"
     gene            4482..4718
                     /locus_tag="VFV20_03620"
     CDS             4482..4718
                     /locus_tag="VFV20_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287466.1"
                     /translation="MDPEILRPAFRIAVLVAGLALATLPFQPRDSAEFVVTVLAAIVG
                     LVFAIGIGLLARLARPPLPPRRDARRKTAGPKPP"
     gene            4787..6577
                     /locus_tag="VFV20_03625"
     CDS             4787..6577
                     /locus_tag="VFV20_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5287467.1"
                     /translation="MTEDETTRRLRYTPSRRRLLRDATFVSLGIIAASCAPGTTGTPP
                     AGGKGKKGGEFHASWPIDLPPKGVWNYYGASPILSGSYLIEIVYQNLAIYRWADKKWD
                     YLLAESHSATASDFTVKLRKGVKWDNGSEVTAKDAVASFKLARLVGSAIWNFATDVEA
                     PDDYTVKFKLSKTSALLERNILRTGIRPASVYGAVADKAWTEFKAGKTAADDSVKAIR
                     KELQDMKVDAPSSNGPYKMDPASLTEAQMTLVRNPGGLFADKVNFDKIVVLNGDTDQI
                     TAPILAGDVDYSTSFFPIASEKAFEAQGIKIVRGPFYTGPALYFHWDNAPQFQDPKMR
                     QAIVWALDRDQANHVAYGEKTQAPQWVAGVSDTLLKSWLSADDQKKLTVYKQDTAKAE
                     SLLKGAGYAKGTDGIWAKDGKKLEFEMQYPSDFADWSAVATNISDQLNKFGIKITPRG
                     APFSTTATDFRNSKFTLAFAPWGTGNPHPQPSMVRPIREWNTQAIGGGQKYPVKQKTS
                     QGDVDFEALLVQAEDIDEAKQKDAVKKYATAFNELLPAIPLAERFNNAPINDQKRVAG
                     WPALDDKIFQNGGGDNFTLILLMDGTLGGK"
     gene            complement(6651..>6863)
                     /locus_tag="VFV20_03630"
     CDS             complement(6651..>6863)
                     /locus_tag="VFV20_03630"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEU5287468.1"
                     /translation="FDIELLGVTVDGQNGQHLGATATVTIDRTDFGLTWNMPVPNGVL
                     VSEKVKVDVDLQAIDEAAAKTRGLAA"
BASE COUNT         1033 a         2496 c         2242 g         1092 t
ORIGIN      
        1 gcacggcgca ccgcgcggcg ggacgagatc gtgaacgctc tcgatctcat ggcgggcgtc
       61 cgcgactacc tcacggacgc tcgccgtctc ggactgcgca tcggcatcgc ctccagctcc
      121 acgcgcggct gggtgtgcgg ccacctcgag cggctcgcgg tgcgcgaggg ctgggacgcg
      181 atcgtttgcc gcgaggacgt ccaggacgcg aagccgtcgc cggagctcta ccagaaggcc
      241 gtcggcctgc tcggcgtcgc tccgcaggag gccctcgcgc tcgaggactc gccgaacggc
      301 atcgcggcgg cgaaggcagc ggggctgcgc tgcgtcgcgg tgccgaaccc gctcaccgct
      361 gatctcgacc tgcgccaggc agacatgcgg atcgcctcgc tcgccgagct ctcgctcggt
      421 gagctcatac gacgactcga cgggtccgcc gtttagcatc acgcgcctat ggccgcggtc
      481 atccccgccg aagagaagct cacatcacgg ctcgacccgc gcgcgcgcct cggcgccgtg
      541 tggcggcacg cgctgctgcg cgcggtgctc aaggcggtgc tcacggtcct cgtcgcgacc
      601 gcgatcacct tcacgctcat ccgcctcctg ccgggcaatc cgatcgacct gcgcatcgac
      661 gagctcacgc gcgacggatc gatgacctac gagcaggcgc gcgagcaggt ctcgggcatc
      721 tatccggtcg acctgaacgc gcccatcccg ctgcagttcg cgagctacct ctggaacgtg
      781 ctgcacggcg acctcggcac gtcgttcctc gcgacgagcg tgccggtgat ggcgatcatc
      841 ctcgccgtcc tcccgtggac gctgttcgcc gtcgggacgg gcctgctgct gagcttcgcc
      901 gtcggcatcc tgctcggcct cctcgccgcg tatcaccggg gctcggcatt cgatcaggcg
      961 gtgaccacga tcgggtcgat cgtgagctcg gtgccgaact acctgatcgc gatcctcatc
     1021 gtgctcatct tcggtatcca gctacggctc atcccgatca cgcagatgcg cggcgccctg
     1081 tcgcccggcg tgcagcccgg cctcacgctc gacttcatcg gcgacgtgct cttccacgcg
     1141 gcgctcccga tcacggtgtt cttcctcacg aacatcggcc attggatcct cagcatgcgc
     1201 aacgcgacgc tcgcggctct cgaggaggac tacgtcgtcg cggcccgcgc gcgggggctc
     1261 tccgagtcgc ggatcacgac cgcgtacgtc ggtcggaacg cggcgctccc cctcgtgacg
     1321 cagctcgcga tctcgatcgg cttcatcgtc ggcggcgcag tcctcatcga gcaggtgttc
     1381 ctttaccagg gcgtcggcct gcgactgctg cgcgccgtcg cgcagcggga ctaccccgtg
     1441 atgcagggca tcgtgctgct cgtcacgatc tccgtggtcg tcgcgaacct cgcagccgac
     1501 ctgctgtaca gcaagctcga tccgcgcatc gggcgcgctt cgtgagcgcc gccctgcgtt
     1561 cggtgcgcct tcccagtttc gtggccgagc tcggcgcgct catcgccgtc atcgcgcgac
     1621 ggcccgccgg tctcgtcggc ctcatcggcg tcggaggatt cctcttcctc gcgtacgtcg
     1681 cgcccttctt cgtgcccctg cacaacgcgg tggacatcgc gaacgtctac cgaacgccgt
     1741 cgctggacca tccgctcggc acggacttcc aggggcgcga cgtgctcaac cagatcgtgt
     1801 acggcggtcg cgacatcctg agcatcgcgt tcctcgccgc gctcttctcc accgcgatcg
     1861 ccgtcacctt cggctcgctc gcggccacgc tcggcggacg gaccgacacg atcgtgctcg
     1921 cggtcgcgga catcgtgctc acggtgccgc atctcatcgt gctcatcgtc gtcgcggcga
     1981 tcttccgacc cgagggcttc ctggtgctcg cgatcctgct ggcgtcgctg cagtgggcgg
     2041 gactgctgcg ccaggtgcgc gcccagatcc tgtcgctcaa ggagcgcgag tacgtggagg
     2101 ccgcgcgctc gctcgacctc ggcctcttcc acgtcatctt ccgcgagatg ctgccgaaca
     2161 tggcgagcta catcgccatc cacttcatct tcgccatgac cggggccgtg tacgcgcagg
     2221 tcggcctcac cgtgctgggt ctcgttccgc tgtcgggcgc gaactggggc gtcatgctgc
     2281 agctcgcgct gcagcagggc gcgatctact tccaggacag cttctggtac gtgctcagcc
     2341 cgatcctcgc gatcgcgctg ttccagctgt cgctcgtcgc gctcgcgtcc gggctcgagg
     2401 acgtcttcaa cccgcggctg cgggccggct gatgccgacg ctgctgtccg tacgcgatct
     2461 ggccatcgag taccgctcgc ggcgcggcgc ggtgcaggcc gtgcgcggcg tgtcgctcga
     2521 ggtcgccgag ggcgagaccg tgtcggtgat cggcgagagc ggatcgggaa agaccacgct
     2581 cgcggtcgcg ctcatccagc tgatcccgcg gagcgctcgt gtgacccgtg gcgaggtgct
     2641 cttccaccac gacggccgcg tcgacgacgt cctcgcgatg ccggccgatg agctgcggca
     2701 cttccgctgg agcgaggccg cgatggtctt ccagtcggcg ctcaactcct tcaaacctgt
     2761 catcgacatc tggtcgcagt tcctcgacac cgcgcgcgcc cacggcatgc gcgacgcgcg
     2821 ggccgtgcgc gatcgcgccg acgagctcct cgaccttgtc catctcgacc cgacgcgcgt
     2881 cctgcgcgcg tatccgcacg agctctcggg cggcatgaaa cagcgcgtgc tcatcgcgct
     2941 cagcctgctc ctccggccgc gcctgctcat cctcgacgag cccacgaccg cgctcgacat
     3001 cctcacgcag cgcgcgatca tcgatctcct gcgcgagctg cgcgagaagc tcggcttcgc
     3061 gatgatcttc atctcgcacg atctgtcgct tgcggcggag ctcgccgacc gcgtgctcac
     3121 gatgtacgcc ggccggatcg tggaggaggc gcgggtcggc gacctcttct acgcgccgcg
     3181 gcatccctac acggtgggac tgctccgcgc ggtgccgcgc gtctcgggcg aggtggggac
     3241 gctctcgtcg atcccgggga cgccgcctga cctgatccac ccgccgcccg gatgcgcgta
     3301 ccacccgcgc tgcccgttcg cgatcgagcg ctgccgcgcg gaggatcccg cgctcgtctc
     3361 cgtagacacg cccgcgcaca ccgcggcgtg catccgctgg caggcggtcg ccgagagcgt
     3421 cggcacgcga cgtcccgccg aggcgctctg atgaccgtgc gcgacgtcac cgcgccgggc
     3481 ccgcatctca tcgagctgga tgccgtcagc cgcagcttcg aaacgcccgg cggccgcatc
     3541 accgccgtcg atcgcgtgtc gctcgcgctc gcggccggcg aggccatctg tctggtgggt
     3601 gagagcggtt gcggcaagac cacgaccgga cgccttctcg cgggcctgct gcgcccgacc
     3661 ggcggaaggc tgctctacga gggcaaggac gtgtgggaga agaagggcgc cgacctcgcc
     3721 gcgttccgcc gcgccgtcca gctcgtccac caggacccct acgcgtcgct gaacccggtg
     3781 cgcaccgtct acgacacgct ctcggcgccg ctgttccggc accacaaggc acgcggccgg
     3841 cgcgaggcga tcgagacggt gcgcgagctc cttcggcggg tcgggctcac gccgcccgag
     3901 gacttcctgc ccaagtaccc gcatcagctc tccggcggcc agcggcagcg cgtcgcgatc
     3961 gcgcgcgccc tctcggtcga tccgcgcgtc gtcgtcgccg acgaggccgt gtccatggtc
     4021 gacgtctcga tccgcatcac gctgctcgac ctgctgctcg ccctgcgaga cgagttcggc
     4081 gtcagcatcg tgctcatcac gcacgacctc gccgtcgcgc ggtacttcgc gcgccgcgga
     4141 cgcatcggcg tgatgtacct cggtcgcgtc gtcgagctcg cgcccaccga ggcgctcgtc
     4201 acggatccgg cgcatccgta cgccgcggcg ctcatcggcg cgatcccgga ggcggacccg
     4261 acgctcaccc gcacgaagga gcgcgtgccg ctccgcggag ccgacgtccc cagcctgctc
     4321 gacgtcccgc cggggtgctc gttccatccg cgctgcccgc tgttcgaagc cgggctctgc
     4381 gacgtgctgg tcccgcggct gctcccgata ccggacgagc gagaggtcgc gtgccatgtc
     4441 gccgtccgcg agcgcaccgg ctcggtggct ggggtctgag cgtggatccc gagatcctgc
     4501 ggccggcgtt ccgcatcgcc gtgctcgtcg ccggcctcgc gctcgcgacg ctccccttcc
     4561 agccgcgcga ttccgccgaa ttcgtcgtta ccgtcctagc ggcgatcgtc gggctcgtct
     4621 tcgcgatcgg catcggcctc ctcgcgcgcc tggctcgacc gccgctgccc ccgcgacgcg
     4681 acgcgagacg gaagacggcc ggacccaagc cgccgtgatg ggcattccac gttgcggaaa
     4741 cgggcccgac cacgtacaac aggcgcttca tcaaggaggg gaacccgtga cagaggacga
     4801 gacgaccagg cgacttcggt acacgccatc gcgacgtcgg ctccttcgcg acgccacctt
     4861 cgtgagcctc ggcatcatcg cggcatcgtg cgcacccgga acgaccggca cgccgccggc
     4921 gggtggcaag ggcaagaagg gcggcgagtt ccacgcctcg tggccgatcg atctcccacc
     4981 caagggcgtg tggaactact acggcgcctc gccgatcctt tccggctcct atctcatcga
     5041 gatcgtctac cagaacctcg cgatctaccg ctgggccgac aagaagtggg actacctcct
     5101 cgcggagtcg cacagcgcga cggcctccga cttcaccgta aagctccgga agggcgtgaa
     5161 gtgggacaac ggcagcgagg tcaccgcgaa ggacgcggtc gcatcgttca agcttgcgcg
     5221 cctcgtcggg tcggccatct ggaacttcgc gaccgacgtc gaggcgccgg acgactacac
     5281 cgtcaagttc aagctctcga agacctcggc gctcctcgag cggaacatcc tccgcacggg
     5341 catccgcccg gcatcggtgt acggcgccgt cgcggacaag gcctggaccg agttcaaggc
     5401 gggcaagacc gccgcggacg acagcgtcaa ggcgatccgc aaggagctcc aggacatgaa
     5461 ggtcgacgcg ccgtcgtcga acggaccgta caagatggat ccggcgtcgc tcaccgaggc
     5521 gcagatgacg ctggtgcgca accccggtgg cctgttcgcc gacaaggtca acttcgacaa
     5581 gatcgtcgtc ctcaacggcg acaccgacca gatcacggcg ccgatcctcg cgggtgacgt
     5641 cgactacagc acgagcttct tccccatcgc gagcgagaag gcgttcgagg cgcaggggat
     5701 caagatcgtg cgcggaccgt tctacacggg tcccgcgctg tacttccact gggacaacgc
     5761 gccgcagttc caggatccga agatgcgtca ggcgatcgtg tgggcgctcg atcgcgacca
     5821 ggcgaaccac gtcgcctatg gcgagaagac gcaggcgccg cagtgggtgg ccggcgtgag
     5881 cgacacgctc ctcaagagct ggctctccgc cgacgaccag aagaagctca ccgtgtacaa
     5941 gcaggacacc gcaaaggcgg aatcactgct gaagggcgcc ggctacgcga agggcaccga
     6001 cggcatctgg gcaaaggacg gcaagaagct cgagttcgag atgcagtatc cgagcgactt
     6061 cgcggactgg tccgccgtcg cgacgaacat ctccgaccag ctgaacaagt tcggcatcaa
     6121 gatcacgccg cgcggcgcac cgttctcgac caccgcgacg gacttccgga acagcaagtt
     6181 cacgctcgcg ttcgcgccgt ggggcaccgg gaacccgcac ccgcagccat cgatggtccg
     6241 gccgatccgc gagtggaaca cgcaggccat cggcggcggc cagaagtacc cggtgaagca
     6301 gaagacgtcg cagggcgacg tcgacttcga ggcgctgctc gtgcaggccg aggacatcga
     6361 cgaagccaag cagaaggacg ccgtgaagaa gtacgccacg gcgttcaacg agctcctgcc
     6421 ggcgatcccg ctggcggagc gattcaacaa cgctccgatc aacgaccaga agcgcgtcgc
     6481 gggctggccg gcgctggatg acaagatctt ccagaacggc ggcggcgaca acttcacgct
     6541 catcctgctc atggacggca cgctcggcgg caagtagcgc gcagcggaca tgaaggaacg
     6601 gcggccccgc gtgatgcggg gccgccgttc gcgtcgagag ggggaacgtt ctaggcggcg
     6661 aggccgcgcg tcttcgcggc cgcctcgtcg atagcctgca ggtcgacgtc gaccttgacc
     6721 ttctccgaca cgagcacgcc gttcggcacc ggcatgttcc aggtgaggcc gaagtcggtc
     6781 cgatcgatcg tcaccgtcgc ggtcgcgccc aggtgctgac cgttctggcc atccaccgtc
     6841 acgccgagca gctcgatgtc gaa
//
LOCUS       DASTZR010000162         2300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1158703, whole genome shotgun sequence.
ACCESSION   DASTZR010000162 DASTZR010000000
VERSION     DASTZR010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158703"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1263)
                     /locus_tag="VFV20_03635"
     CDS             complement(91..1263)
                     /locus_tag="VFV20_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5287469.1"
                     /translation="MLTVTDLRITYRGMQGTVEALRGVSFEVDEGEFFTLLGPSGCGK
                     TSTLRCIAGLETPTSGTIAVGGATLFSTGPKRVNVAAHRRGFGMVFQSYAIWPHMSVF
                     DNVAFPLIHGVQRLGKREARERALNALGLVQLQDVADRPAPYLSGGQQQRVALARALA
                     LEPKVLLLDEPLSNLDAKLRDEMRHELKTLVRRLGITTLYVTHDQLEALSMSDRVAVM
                     HAGVIEQLGRPRDVYQAPASSFTARFLGRTNVLDGTANAHGVTTQWGAFRCEVPEWAT
                     PGARVALGFRPESITITREAREGLANVLVGSIVGHTFNGESAEYVVDITGKTVQARGD
                     AFDIIAEGARAYLTIPPERCFVLALSEHHKVAGGSTDATDESVVSIIPDAQGRVLP"
     gene            complement(1263..>2300)
                     /locus_tag="VFV20_03640"
     CDS             complement(1263..>2300)
                     /locus_tag="VFV20_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5287470.1"
                     /translation="DVPAILGMPAQIYTLSTKVYLLTQTGNYAAASVLGVVFLSAAVV
                     VVLLYARAVRGIERFSTITGKGYRPRAMPLGRLRYPALALVALYLFVVAAAPILITLW
                     ASVLPFYRAPSVDAIQYVTFESYTRLFETPRRAVTVWNSLIVTFAAPTIALLVVTPIS
                     WFVVRTRIRGRRLLDIIAFLPHTVPSSVIALAVVYLFLGTPWGLLPIYGTAWIIVFAL
                     AVRHFAYGSRQMHSAMIQLHKDLEEAGYVSGVPWRKTLTRIIVPLVAPSVIAAWVFMA
                     MTSVRDTTMALMLGSTSNRVLPLLMWDAWQIGSYNDAAAGGMVLLAFVVVILVVGAIV
                     QRTRIGRPGAA"
BASE COUNT          381 a          760 c          818 g          341 t
ORIGIN      
        1 cgatgcggtt gccgtcaggg ccgacgagct gcttcttccc cttcaccgcg ggcttgtatc
       61 gcggcagcga gtggatctcg ggtcggatgg tcatggcagg accctccctt gggcgtcggg
      121 gatgatgctc acgacgctct cgtcggtggc atccgtcgac ccgcccgcga ccttgtgatg
      181 ttccgagagc gcgagcacga agcagcgctc cggcgggatg gtgaggtacg cacgtgcgcc
      241 ctcggcgatg atgtcgaacg cgtcgccgcg tgcctggacc gtcttcccgg tgatgtcgac
      301 gacgtattcc gcgctctcgc cgttgaacgt gtggcccacg atcgatccga cgagcacgtt
      361 cgccagaccc tcgcgcgcct cccgcgtgat cgtgatgctc tcggggcgga agccgagcgc
      421 gacgcgcgca ccgggcgtcg cccactcggg cacctcgcag cggaacgctc cccactgcgt
      481 cgtcacgccg tgcgcgttcg ccgtgccgtc gagcacgttc gtgcggccga ggaatcgcgc
      541 cgtgaacgag gacgccggcg cctgatagac gtcgcggggc cgcccgagct gctcgatgac
      601 gccggcgtgc atcacggcga cgcggtccga catgctgagc gcctccagct ggtcgtgcgt
      661 cacatagagc gtcgtgatgc cgagacgccg gacgagcgtc ttcaactcgt ggcgcatctc
      721 gtcacgcagc ttcgcatcga gattgctgag cggctcatcg aggaggagca ccttcggctc
      781 gagcgccagc gcgcgcgcga gcgccacgcg ctgctgctgc ccgccggaca ggtacggcgc
      841 cgggcgatcg gcgacgtcct ggagttgcac gaggccgagc gcgttcagcg cgcgctcgcg
      901 cgcctcgcgc tttcccagcc gctgcacgcc gtggatgagc gggaacgcca cgttgtcgaa
      961 cacgctcatg tgcggccaga tcgcgtacga ctggaatacc atgccgaatc cgcggcgatg
     1021 cgccgcgacg ttcacgcgtt tcgggccggt cgagaagagt gtggcgccgc cgaccgctat
     1081 cgtgccggac gtcggtgtct cgagcccggc gatgcagcgc agcgtgctcg tcttcccgca
     1141 ccccgatggg ccgaggaggg tgaagaactc gccctcgtcg acctcgaacg agacgccgcg
     1201 gagcgcctcg acggtaccct gcatgccccg gtaggtgatg cgcagatcgg tgacggtgag
     1261 catcacgcgg ctcccgggcg gccgatgcgc gtgcgctgga cgatcgcgcc gacgacgagg
     1321 atgacgacga cgaacgcgag gagcaccatg ccgccggcgg ccgcgtcgtt gtacgacccg
     1381 atctgccatg catcccacat cagcaggggc agcacgcggt tgctcgtcga gccgagcatg
     1441 agcgccatcg tcgtgtcgcg gacggaggtc atggccatga agacccacgc ggcgatcacc
     1501 gacggcgcga cgagcgggac gatgatcctg gtgagcgtct tgcgccacgg cacgccgctc
     1561 acgtagccgg cctcttcgag atccttgtgc agctggatca tcgcgctgtg catctggcgg
     1621 ctgccgtacg cgaagtgccg gaccgcgagc gcgaagacga tgatccacgc ggtgccgtag
     1681 atcgggagga ggccccacgg cgtcccgagg aacagataga ccacagcgag cgcgatcacg
     1741 ctcgatggca cggtgtgcgg caggaaggcg atgatgtcga gcagccgcct accgcggatg
     1801 cgcgtgcgca cgacgaacca cgagatcggg gtgacgacga ggagtgcgat cgtcggggcg
     1861 gcgaacgtga cgatgaggct gttccagacc gtcaccgcgc ggcggggcgt ctcgaagagc
     1921 cgcgtgtacg actcgaacgt gacgtactgg atcgcatcaa cggacggcgc gcggtagaac
     1981 gggagaacgc tcgcccagag cgtgatcagg atcggcgcgg cggccacgac gaacaggtac
     2041 agcgcgacga gcgccagcgc ggggtagcgc agccggccga gcggcatcgc cctcgggcga
     2101 tagcccttgc cggtgatcgt gctgaagcgt tcgatgccgc ggacagcacg tgcgtagaga
     2161 agcaccacga ctactgccgc cgagaggaac accacgccga ggaccgaggc ggcggcgtag
     2221 ttgcccgtct gcgtcagcag gtacaccttc gtcgagagcg tgtagatctg cgcgggcatg
     2281 ccgaggatcg ccggtacatc
//
LOCUS       DASTZR010000163         2497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1286612, whole genome shotgun sequence.
ACCESSION   DASTZR010000163 DASTZR010000000
VERSION     DASTZR010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286612"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VFV20_03645"
     CDS             <1..737
                     /locus_tag="VFV20_03645"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5287471.1"
                     /translation="SELRQTAARLAERVDAELQFEAILGHELRTPLLAIMSFAALLKR
                     NGMSMDRVKELAGYLEQDAERAQRLIDQLLDLDRVESGRAPLLIERVNVYDALSQVVA
                     TLSPASPRHPFRLAGARDAVVLADRDRLIQVFTNLLVNAVKYSPDGGDVEIDVTSESG
                     RVHVRVRDHGLGIPPTELERIFQKHTRIHTARDIKGSGLGLQLVRAIVELHGGRVWAE
                     SEGEGSGATLNVLLSGAPAVDAEAPR"
     gene            734..1399
                     /locus_tag="VFV20_03650"
     CDS             734..1399
                     /locus_tag="VFV20_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5287472.1"
                     /translation="MTGDIRILIVEDHPIIAAGLQALLDDHEGYTVAAVAGTVADGLE
                     VARREQPKVAIVDYRLPDGTGAELASALRVACPDTAVIILTAEAGDDALLDSASAGAT
                     GYVRKSEAPARLVNAIESVLAGRNALPVHELARALQADRLRQQERRELDAVRSSLTPR
                     ELEILTLMARGRDNRAIAAELTVGYTTVRSHVQSIIEKLGARSRLEAVARATEMGIIG
                     VER"
     gene            complement(1421..1681)
                     /locus_tag="VFV20_03655"
     CDS             complement(1421..1681)
                     /locus_tag="VFV20_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5287473.1"
                     /translation="MSLITDEPVTSTTTTEMRRLTQRQRQIVGMIARGMADKEIAAAL
                     GIATATAEKHVANVLLRLQVPNRAAAVFKAYVGEPDEEPVLG"
     gene            2186..>2497
                     /locus_tag="VFV20_03660"
     CDS             2186..>2497
                     /locus_tag="VFV20_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU5287474.1"
                     /translation="MAASPLNDVHGSFIDETIGIVARQTRDTVSFAVGSPSREALDLV
                     GAEDLARAVIAREGASALGYTITEGEPELREAVADAARRRGMRADAEEVLISAGALQG
                     ID"
BASE COUNT          390 a          874 c          867 g          366 t
ORIGIN      
        1 agagcgagct gcgtcaaacg gccgcgcgcc ttgccgaacg cgtcgacgcg gagctccagt
       61 tcgaggcgat cctcggccac gagctgcgga cgccgctgct cgccatcatg tcgttcgccg
      121 cgctgctgaa acgcaacggc atgtccatgg accgcgtgaa ggagctcgcg ggctatctgg
      181 agcaggacgc agagcgtgcg cagcggctca tcgaccagct cctcgatctc gatcgggtcg
      241 agtccggacg cgcaccgctc ctgatcgagc gcgtgaacgt atacgacgct ctctcgcagg
      301 tcgtcgcgac gctctcaccg gcatcgccgc ggcacccatt caggctggcg ggagcgcgcg
      361 atgcggtggt cctcgcggat cgcgaccgcc tcatccaggt cttcacgaat ctgctcgtga
      421 acgcggtgaa gtactcgccg gacggcggtg acgtcgagat cgacgtgacg agcgaatcag
      481 gtcgagtgca tgtccgcgtc cgcgatcacg gcctcggcat cccaccgacg gagctcgagc
      541 ggatcttcca gaagcacacg cgcatccaca ccgcgcgcga catcaaaggg agcggcctcg
      601 gcctgcagct ggtgcgagcg atcgtcgagc tgcatggcgg ccgcgtgtgg gccgagagcg
      661 agggcgaggg cagcggcgcg acgctgaacg tccttctcag cggcgcgccg gccgtggacg
      721 cggaggcgcc gcgatgaccg gggacatccg catcctcatc gtcgaggacc atccgatcat
      781 cgcggccggc ctgcaggccc tgctcgacga ccacgagggg tacaccgtcg cggcggtcgc
      841 ggggacggtc gcggacggtc tcgaggtcgc gcggcgcgag cagccgaagg tcgcgatcgt
      901 cgactacagg cttcccgacg gcaccggcgc agagctcgcg tcggcgctcc gcgtcgcctg
      961 tccggacacc gccgtcatca tcctcaccgc cgaggccgga gacgacgcgc tgctcgattc
     1021 cgcctccgcc ggcgcgacgg gctacgtgcg caagtccgag gcgcccgccc gactcgtgaa
     1081 cgcgatcgag agcgtcctgg ccgggcgcaa tgccctgccg gtacacgagc tcgcacgggc
     1141 gctccaggcc gaccgtctcc ggcagcagga gcggcgtgag ctcgacgcgg tgcgaagctc
     1201 gctcacgcca cgcgagctgg agatcctcac gctgatggcg cgcggacgag acaatcgggc
     1261 gatcgcggcg gagctcacgg tcggctacac gaccgtccgc tcgcacgtac agagcatcat
     1321 cgagaagctc ggagcgcgct cgcgcctcga ggccgtcgcc cgcgcgacgg agatggggat
     1381 catcggcgtc gagcgctgat gggctccgcg gagcccggcg tcagccgagg accggctcct
     1441 cgtcgggctc gccgacgtag gccttgaaga ctgcagcggc gcggttgggc acctgcagac
     1501 ggagcaggac gttcgcgacg tgcttctccg cagtcgccgt cgcgatcccg agggctgccg
     1561 caatctcctt gtcggccatc ccccgcgcga tcattccgac gatctgacgc tgtcgctgcg
     1621 tcagccgccg catctctgtc gtggtcgtgc tcgtcacggg ctcgtccgtg atcaggctca
     1681 tcgcatgcct cgctttccct cgaccttcgt cggagggcag ggccgcatcg aagcctgcct
     1741 gttccgtaga tgaggtctgt gctcgccgag catcgacgat ggggggccga cacacctcgg
     1801 gcgagttacc gatttcgcag ccccgcgctc gtacccgccg tgcctcccac cgtcgtatga
     1861 ggacgtatgt tcacgtggcg atcgcgagcg agcgcggcac cgcggaccgt cggggacagg
     1921 ctggttgtgc atgctgaata acccgatccg ggcgcgttga cccggtctga ccgcgaccga
     1981 taccgttccc cgcgatggcc ctcatcccgt tcgtcgtcct catcgttccg gccccgatcc
     2041 cggggtctgg cacacgcacg gggggctagc gcaacaccac acacacaggc aacgcgacac
     2101 caaaggcctc ccaaaacggg aggccttttc agttgaagga agggaggcga ccgacatggc
     2161 agtccgagag atcactgggc gagaggtggc cgcctccccg ctcaacgacg tccacgggtc
     2221 gttcatcgac gagaccatcg gcatcgtcgc gcggcagacg cgcgacacgg tctcgttcgc
     2281 cgtcggctcg ccgtcgcgcg aggcgctcga cctggtcggc gcggaggatc tcgcgcgcgc
     2341 ggtcatcgcc cgcgagggcg cgagcgcgct tgggtacacg atcacggagg gcgagccgga
     2401 gctgcgcgag gccgtcgccg acgccgcgcg ccggcggggg atgcgtgcgg acgccgagga
     2461 ggtgctcatc agcgcgggcg cgctccaggg catcgac
//
LOCUS       DASTZR010000164         3834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_218235, whole genome shotgun sequence.
ACCESSION   DASTZR010000164 DASTZR010000000
VERSION     DASTZR010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218235"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VFV20_03665"
     CDS             complement(<1..212)
                     /locus_tag="VFV20_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU5287475.1"
                     /translation="MTRAVLFHGGRIHVGDGRSAEALLQRDGRVAAVGTARELRRASS
                     DAELIDLRGGLMTPGWMDAHVHFVWWS"
     gene            complement(209..832)
                     /locus_tag="VFV20_03670"
     CDS             complement(209..832)
                     /locus_tag="VFV20_03670"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEU5287476.1"
                     /translation="MGVTRDEMVRHLRQLGIRDERVLAAMGRVPREEFVRWEDREVAY
                     GDHALPIEVGQTISQPYVVARMTELLDVDGSHHVLEVGTGSGYQAAVLAELARDVVSV
                     ERHGELARTASARLARLGYTNVRVIHDDASLGRPEDAPYERIVVTAGAPAIDPALAAQ
                     LAPGGRLVAPVGDTQMQELVVRDATGHEERHGAVRFVPLRGRGGFQP"
     gene            complement(865..1620)
                     /locus_tag="VFV20_03675"
     CDS             complement(865..1620)
                     /locus_tag="VFV20_03675"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU5287477.1"
                     /translation="MAIRAAFFDVGDTLVEHWAPRDQLRELLREALRREFGERHWYED
                     FLRAEVAPAVPAVSAVGELDPGEEEALRQETLRWYEDWFRNAAIGVDDIEMDRLRVAM
                     TVPLDLVSTPVPGAFTAVRWCKQQGLTVGLITNTLSRGDDEVREDWRRLGLADAIDHV
                     VSSHSAGWQKPHRKIFERALALAGVAAGDAFMVGDRLDADIWGASRMGMRTVLRRTEH
                     TQPHVDVHPDAAVDDLTELPGVVEPWLGMRRSK"
     gene            1643..2092
                     /locus_tag="VFV20_03680"
     CDS             1643..2092
                     /locus_tag="VFV20_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287478.1"
                     /translation="MDFEQLAFIEKWRQKASRGVVVTLDGTRGDIVVTLRVGELGERL
                     DLRGQDATGAVRKQRLAIGDRVFMAFEYRAKDARTGTGEAGVSGGLVAPNAIVRGTVA
                     EAGDTTVVDCGAPVLVRGDTLPNVRVGDAVEFTVDGEGKAYLIPTRG"
     gene            complement(2100..2606)
                     /locus_tag="VFV20_03685"
     CDS             complement(2100..2606)
                     /locus_tag="VFV20_03685"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(2430..2432),aa:Sec)
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEU5287479.1"
                     /translation="MAAPKGRNKIAVVTKTLRVGDRAPEFRLRAHDGTEVALSDLRGR
                     RVVLAFFAFAFTNTUNSEAPALNALQERFAGGTAQVVGISCDTVYSLKVWAEQLGGVK
                     YPLCSDFWPHGEVTRAYGVFNDDIGRPERAIIVVDAEGVVRYIDVHQLREVPDDEEIA
                     EELRKLEG"
     gene            2652..2864
                     /locus_tag="VFV20_03690"
     CDS             2652..2864
                     /locus_tag="VFV20_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287480.1"
                     /translation="MSVDQRRRIDADRHAFLAYGVACPFCGEKVGPRFVTREHLDVPP
                     NPPYAATVKCPKCREIFEVRFGDSAR"
     gene            complement(2887..>3834)
                     /locus_tag="VFV20_03695"
     CDS             complement(2887..>3834)
                     /locus_tag="VFV20_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287481.1"
                     /translation="TRLPIYYAPQQFERDYQVIVPQRPLAANTTYHVRFDINVNGRMV
                     TNEWDFSTGATLGASIPTDSGLHSAWVSQSAVAALQPAATSAVTLQFRNTGTKTWQRG
                     VAGSQIALGVPGDSTAYAALGMNVGWPSANRVAIQNEASVAPGGTASFTFTVRAPLAA
                     GTVALRVRPVVDGVAWLEDQGVYVPVTTRVDYHSAWMAQSPYPTLQPGVTSGVLTLTF
                     RNTGTQTWTRGVLGQEARLAVNRDDTMWAALGVGWLSANRVAAQNEASVAPGAAATFS
                     FQVRAPSTPGVYSINLRPVIDGVTWMEDQGVFLIVTVAP"
BASE COUNT          563 a         1393 c         1316 g          562 t
ORIGIN      
        1 gaccaccaca cgaagtggac gtgcgcgtcc atccatcccg gtgtcatgag cccgccgcgc
       61 aggtcgatca gctcggcatc ggacgacgcg cgccgtagct cacgcgccgt acccaccgcg
      121 gccacgcgcc cgtcgcgctg caggagcgcc tcggccgatc tcccatcgcc gacgtggatc
      181 cggccgccgt ggaacaggac cgcgcgcgtc acggctggaa tcctccgcgt ccgcgcagcg
      241 ggacgaagcg caccgcgccg tgccgctcct cgtggcccgt cgcgtcgcgc accacaagct
      301 cctgcatctg ggtgtctccg accggcgcca ccagccgacc gcccggtgcg agctgcgccg
      361 cgagcgccgg gtcgatcgcc ggcgcgcccg cggtcacgac gatgcgctcg tagggcgcgt
      421 cctcgggccg gccgagcgag gcgtcgtcgt ggatcacgcg gacgttcgtg tagcccagcc
      481 gggccagtcg tgcgctcgcg gtccgcgcga gctcgccgtg ccgttcgacg ctgacgacat
      541 cgcgcgcgag ctcggcgagc accgcggcct ggtagccgga tcccgttccg acctcgagca
      601 cgtggtggct gccgtcgacg tcgaggagct ccgtcatgcg tgcgaccacg tatggctggg
      661 agatcgtctg tcctacctcg atcggcagcg cgtggtcgcc gtacgccacc tcgcggtcct
      721 cccagcggac gaactcctca cgcgggacgc ggcccatcgc cgcgaggact cgctcgtcgc
      781 ggatccccag ctgtctcagg tgccgcacca tctcatcgcg cgtgacgccc acgcgcacat
      841 gatgcggccc gacggcccct cagctcattt gctccgacgc atgcccagcc acggctcgac
      901 gacgcccggc agctcggtga ggtcatcaac ggcggcgtcc ggatgcacgt ccacgtgtgg
      961 ctgcgtgtgc tcggtgcgcc gcagcacggt ccgcatgccc atgcgcgacg cgccccagat
     1021 gtccgcgtcg agacgatcgc ccaccatgaa cgcgtcaccc gcggccacgc cggcgagcgc
     1081 gagcgcgcgc tcgaagatct tccggtgcgg cttctgccat ccggcgctgt gcgagctcac
     1141 gacgtggtcg atcgcgtccg ccagtccgag ccgccgccag tcctcccgga cctcgtcgtc
     1201 gccgcgtgac agcgtgttgg tgatcaggcc gacggtgagg ccctgctgct tgcaccaccg
     1261 gaccgccgtg aacgcgccgg gaacgggcgt cgacacgagg tcgagcggga ccgtcatcgc
     1321 gacgcgcaac cggtccatct cgatgtcgtc gacgccgatc gcggcgttcc gaaaccagtc
     1381 ctcgtaccag cggagcgtct cctggcgcag cgcctcctcc tcaccgggat ccagctcccc
     1441 cacggcggag accgcgggca cggcgggcgc gacctccgca cgcaggaagt cctcgtacca
     1501 gtgccgttcg ccgaactccc gtcggagcgc ctcgcggagc agctcgcgca gctggtctcg
     1561 cggcgcccag tgctcgacca gcgtgtcgcc cacatcgaag aacgcggcac ggatggccac
     1621 gtgggtatcg tacggaccaa tgatggattt cgagcagctg gcattcatcg agaagtggcg
     1681 gcagaaggcg tcgcgcggcg tcgtcgtgac gctcgacggc acgcgcggcg acatcgtcgt
     1741 gacgctgcgg gtcggcgagc tcggcgaacg actcgacctg cgtggtcagg acgcgaccgg
     1801 cgccgtgcgc aaacagcgtc tggcgatcgg cgaccgggtc ttcatggcat tcgagtaccg
     1861 cgcgaaggac gcgcggaccg gtacgggcga ggccggcgtg agcggagggc ttgtcgcgcc
     1921 gaacgcgatc gtccgcggga ccgtggccga ggccggcgac acgaccgtcg tcgactgcgg
     1981 cgcgccggtc ctggtgcggg gcgacacgct cccgaacgtg cgcgtgggag acgccgtcga
     2041 gttcacggtc gacggtgagg gcaaggcgta cctcataccg acgcgcggct aggcggagtt
     2101 cagccctcga gcttccgcag ctcctcggcg atctcctcgt cgtcgggtac ctcccgcagc
     2161 tgatgcacgt cgatgtagcg aacgacgccc tcagcatcca cgacgatgat cgcccgctcg
     2221 ggccggccga tgtcatcgtt gaacacgccg taggcccgcg tgacctcacc gtgcggccag
     2281 aagtccgagc acaacgggta cttcacgccg ccgagctgct cggcccagac cttcagcgag
     2341 tacaccgtgt cgcacgagat gcccacgacc tgggcggtgc cgcccgcgaa ccgctcctgg
     2401 agcgcgttca gcgcgggcgc ctcgctgttt caggtgttcg tgaatgcgaa cgcgaagaac
     2461 gcgagcacga cccgccggcc gcggaggtcg gagagcgcga cctcggtgcc gtcgtgcgcg
     2521 cgaagacgga actccggtgc ccggtccccc accctcagcg tcttcgtcac gaccgcgatc
     2581 ttgttccgac ccttcggggc agccacgttc gtacactagc cgagcgaacg aacgaggagg
     2641 cccgaccgca catgagtgtt gaccagcggc gccggatcga cgccgaccgc cacgccttcc
     2701 tcgcctacgg agtcgcatgt ccgttctgcg gcgagaaggt cgggccgcgc ttcgtcacgc
     2761 gcgagcatct cgacgtgccg ccgaatccgc cctacgcggc gacggtcaag tgcccgaagt
     2821 gcagggagat cttcgaagtg cgcttcggcg attcggcgcg ctaagagccg gcacagcgcg
     2881 ttcgcgctac ggcgcgacgg tcacgatcag gaagacgccc tgatcctcca tccacgtcac
     2941 gccatcgatc accggccgta ggttgatgct gtacacgccg ggcgtgctcg gcgcgcgcac
     3001 ctggaagctg aacgtcgcgg ccgccccggg ggccacgctc gcctcgttct gcgccgcgac
     3061 gcggttcgcg gagagccagc ccaccccgag ggccgcccac atggtgtcgt cgcgattgac
     3121 ggcgagccgg gcctcctggc ccagcacgcc acgcgtccag gtctgcgtcc cggtgttccg
     3181 gaacgtgagc gtgaggacgc cgctggtgac acccggctgc agcgtcggat atggcgactg
     3241 cgccatccag gcgctgtggt agtcgacgcg tgtcgtgacc gggacgtaga ccccctggtc
     3301 ctcgagccac gcgacgccgt ccacgactgg gcgcacgcga agcgcgaccg tcccggcggc
     3361 gagcggcgcg cggacggtga aggtgaagct cgccgtgccg cccggagcca cgctcgcctc
     3421 gttctggatg gcgacgcggt tcgcggacgg ccaccccacg ttcatgccca gcgcggcgta
     3481 ggccgtgctg tcgccgggta cgccgagcgc gatctgactg cccgccacgc cgcgctgcca
     3541 cgtcttcgtt ccggtgttgc ggaactggag cgtgaccgcg gacgtcgccg ccggctgtag
     3601 cgcggcgacg gcggactggc tcacccacgc ggagtgcaga ccgctgtcgg tggggatgga
     3661 cgcgccgagc gtcgcacccg tgctgaagtc ccactcgttc gtgaccatgc ggccgttcac
     3721 gttgatgtcg aagcgcacgt gataagtggt gttcgcggcc agcggtcgct gcggaacgat
     3781 gacctggtag tcgcgctcga actgctgcgg cgcgtaatag atcggcagcc gcgt
//
LOCUS       DASTZR010000165         2246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_661707, whole genome shotgun sequence.
ACCESSION   DASTZR010000165 DASTZR010000000
VERSION     DASTZR010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661707"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="VFV20_03700"
     CDS             complement(<1..1108)
                     /locus_tag="VFV20_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEU5287482.1"
                     /translation="MYRPQNLRIPGPTVVPQTVLAASARPMINHRGPEFAALLAALTR
                     ALQDFLRTQGDVLTLTASGSGAMEAAVANTLSRGDKALVFINGAFGERFAAICKAYGV
                     DARRVDVPLGRHVEPDLVRDELAKEKGKDGAKAVLIQHNETSTGVLNPIKEISDVVRE
                     SGKLFIVDSISGAGAAELEIDEWGIDVCVTGSQKAWGAPPGVSIITMSEKAWKAYEKS
                     DLPKAYFDLAAYKAALEKGATPATPAVSVYIALQESLRLMAEEGLEAILRRHRDIARA
                     TRRGLQAVNLALFADEAHASPAVTAFRPPTRVDARNLIRVLRDDYDTIVAGGQGKLEG
                     QLVRVGHLGFVTMTDMVAFFSALELALRDFNQPVE"
     gene            1151..1813
                     /gene="rpe"
                     /locus_tag="VFV20_03705"
     CDS             1151..1813
                     /gene="rpe"
                     /locus_tag="VFV20_03705"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU5287483.1"
                     /translation="MKLAPSILSADFGQLAQAVRACERGGADWVHVDVMDGHFVPNLT
                     FGPKMVADLARATRLPLDVHLMIERPDDWVDRFVEAGAAYVTVHIEAVRDVRDTLVRI
                     RRGGAGAGLTLNPETAVEAVLPFLGELDLLLVMSVHPGFGGQAFIEEALDKVRTVRTA
                     IDAAGLRTELEVDGGVKLHNARRVAEAGASVIVAGSAVFEDGDGPEAAIARFRKTLGM
                     DR"
     gene            complement(1817..2188)
                     /locus_tag="VFV20_03710"
     CDS             complement(1817..2188)
                     /locus_tag="VFV20_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018547463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEU5287484.1"
                     /translation="MSVLKRARFARLVRRALDDLPPQFRDRMRNIEIVIDDEPSEEQR
                     PRDGSELLGLYEGVPLTGRGAMEPYVPDRISIFRGPIERMSSSPRRQAEIVRDTVVHE
                     IAHHFGISDERLRELGRGDAE"
BASE COUNT          306 a          740 c          838 g          362 t
ORIGIN      
        1 gctcgaccgg ctggttgaag tcgcggagcg cgagctcgag agcgctgaag aacgcgacca
       61 tgtcggtcat cgtgacgaag ccgaggtggc cgacgcgcac gagctggccc tccagcttgc
      121 cctggccgcc ggcgacgatg gtgtcgtagt cgtcgcgcag cacgcggatc aggttccgcg
      181 cgtcgacgcg cgtcggcggg cggaacgcgg tgaccgcggg cgaggcgtgc gcctcgtcgg
      241 cgaagagggc gaggttcacg gcctggaggc cgcgacgcgt ggcgcgcgcg atgtcgcggt
      301 ggcggcgaag gatcgcctcg aggccctcct cggccataag ccgcaggctc tcctgcagcg
      361 cgatgtacac gctgaccgcc ggcgtcgcgg gcgtcgcgcc cttctcgagc gcggccttgt
      421 acgcggcgag gtcgaagtac gccttcggca gatcgctctt ctcgtaggcc ttccaggcct
      481 tctcgctcat ggtgatgatc gagacgccgg gaggcgcgcc ccacgccttc tgcgatccgg
      541 tcacgcagac atcgatgccc cactcgtcga tctcgagctc ggccgcgccg gcacctgaga
      601 tgctgtcgac gatgaagagc ttcccggact cgcggacgac gtcggagatc tccttgatcg
      661 gattgagcac gccggtcgag gtctcgttgt gctggatgag cacggccttc gcgccgtcct
      721 tgcccttctc cttcgcgagc tcgtcgcgga cgaggtcggg ctcgacgtgc cgtccgagcg
      781 ggacgtccac gcggcgggcg tcgacgccgt acgccttgca gatcgcggcg aaccgctcgc
      841 cgaacgcgcc gttgatgaag acgagggcct tgtcgccgcg ggacagcgtg ttcgcgacgg
      901 cggcctccat cgctccggag cccgaggccg tgagcgtgag gacgtcgccc tgcgtgcgca
      961 ggaagtcctg cagcgcgcgc gtcagcgcgg ccaggagggc ggcgaactcg gggccgcggt
     1021 ggttgatcat cggtcgcgcc gatgcggcga ggacggtctg ggggacgacg gtggggccgg
     1081 ggatgcgcag gttctgtggg cgatacacgg gcgatttctc ctcggggcgt gctcgacgca
     1141 tgatgccagc gtgaagctgg ctccgtccat cctttccgcc gatttcgggc aattggctca
     1201 ggcggtccgc gcctgtgagc gcggcggcgc cgactgggtc cacgtggacg tgatggacgg
     1261 ccatttcgtg ccgaatctca cgttcgggcc gaagatggtc gccgacctgg cgcgggccac
     1321 gcggctgccg ttggacgtgc acctgatgat cgagcgcccc gacgactggg tggatcgctt
     1381 cgtcgaggcc ggcgcggcgt atgtgacggt ccacatcgaa gccgtgcgcg acgtgcggga
     1441 cacgctcgtc cgcatccgcc gcggcggcgc gggcgccggt ctcaccctga atcctgagac
     1501 cgcggtggag gccgtgcttc cgttcctcgg cgagctggac ctcctgctgg tgatgagcgt
     1561 ccaccccggc ttcggtgggc aggcgttcat cgaggaggcg ctcgacaagg tgcggacggt
     1621 ccggacggcc atcgacgcgg ccgggctgcg cacggagctc gaggtggacg gcggcgtgaa
     1681 gctgcacaac gcgaggcgtg tggccgaggc cggcgcgtcg gtcatcgtcg ccggctcggc
     1741 ggtgttcgag gatggcgacg ggcccgaggc cgcgatcgcc cggttcagga agacgctcgg
     1801 gatggatcgc taaggcctac tcggcgtcgc ctcggccgag ctcccgcagc cgttcgtcgc
     1861 tgatcccgaa gtggtgcgcg atctcgtgca cgacggtgtc gcgcacgatc tcggcctgcc
     1921 gccgcggcga cgacgacatg cgctcgatcg gcccgcggaa gatcgagatg cgatcgggta
     1981 cgtacggctc catcgcgccg cgcccggtga gcggcacacc ttcgtacagg ccgagaagct
     2041 cgctgccgtc acgcgggcgc tgctcctcgc tcggctcgtc gtcgatgacg atctcgatgt
     2101 tgcgcatgcg gtcgcggaac tgcggcggca ggtcatcgag cgcgcggcgc accaggcgcg
     2161 cgaatcgcgc gcgcttcagg accgacatgc cggccgacgc cttcgggtcc gaggatccat
     2221 ttgggcaaag tgtcatccgt agcggg
//
LOCUS       DASTZR010000166         2317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_347399, whole genome shotgun sequence.
ACCESSION   DASTZR010000166 DASTZR010000000
VERSION     DASTZR010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347399"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="VFV20_03715"
     CDS             <1..1250
                     /locus_tag="VFV20_03715"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEU5287485.1"
                     /translation="QTLGWNPDLARGTLRLLARYQGQKVDEFTEEEPGKIFHELRRGE
                     LARLKEIPHRPYYGSVDATPLFALVFAESVKWTADRALWRELLPAAERALTWCDGPWG
                     DPDRDGYVEYGMRATSLRSQGWKDASDSLSDPDGSWSELPAALVEVQSYLYAAKMGLA
                     DLYELDGDAKRAERLRAEASGLRERFERDFWMPKEGCYAQALDGHKKQVPAVTSNAAH
                     ALWCGIASEDHAATLVRRLMEPDMYTGWGLRTLSSRYPTYNPMSYHNGSVWPHDSAIA
                     AHGMSRYGFREEANEVVSGLIEAGRRFPNARLPELFCGFQRDLRFSARPADYPVSCIP
                     QAWSAGVVFLCLRTLLGAEPDLRTQRLLLDPALPPWLERIDVRDLRMFDAPVSFRVRR
                     HRTGDRVAGGRGRVTRSRVTSPA"
     gene            1298..>2317
                     /locus_tag="VFV20_03720"
     CDS             1298..>2317
                     /locus_tag="VFV20_03720"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5287486.1"
                     /translation="MLGGVEQVMGYHAAGLRDAGATVTILAGRGGAPRGVTIARIRDV
                     DSRSAAVERDRIALLRGRRTAEHDALVARLVARIGPLVAKADRVVVHNVMTMPMNLAL
                     TEALARLAADHPGRFIAWTHDLARYDARYDGYRHPGEPWDLVERALPGFRYVTVSSER
                     AAQLAELTGLARERISVVTNGIDVPTAIGLAPESRDLFRRFQFADADPLLLLPARVTP
                     RKRIEAAIDATAALRRRGHRPMLVVTGGVGPHDARNRAYLAALVERAKNVEGVVLLAA
                     HGITIDYHVVVDLYGLADVLVFPSAHEGFGLPVLEAGLHRLPIVCSDIPALREIGGDE
                     PHYIPP"
BASE COUNT          309 a          867 c          811 g          330 t
ORIGIN      
        1 accagaccct cgggtggaac ccggacctcg cgcgggggac gctgcggctg ctcgcgcgct
       61 accaggggca gaaggtggac gagttcaccg aggaggagcc cgggaagatc ttccacgagc
      121 tgcggcgcgg cgagctcgcg cgcctcaagg agatcccgca ccgcccctac tacggctccg
      181 tcgacgccac gccgctcttc gcgctcgtct tcgcggagtc cgtgaagtgg accgcggacc
      241 gcgcgctctg gcgcgagctg ctgcccgccg ccgagcgcgc gctgacgtgg tgcgacggcc
      301 cgtggggcga tccggatcgc gacgggtacg tcgagtacgg catgcgcgcg acgtcgctgc
      361 gcagccaggg ctggaaagat gcgagcgact cgctctccga cccggacgga tcgtggtccg
      421 agcttcctgc ggccctcgtc gaggtccagt cgtatctcta cgcggcgaag atgggcctcg
      481 ccgatctgta cgagctcgac ggcgacgcca agcgcgcgga gcggctgcgc gcggaggcga
      541 gcgggctgcg cgagcgcttc gagcgcgact tctggatgcc gaaggaaggc tgctacgccc
      601 aggcgctcga cggccacaag aagcaggtgc ccgccgtgac gagcaacgcc gcgcatgcgc
      661 tctggtgcgg catcgcgagt gaggaccacg ccgccacgct cgtacgccgg ctcatggagc
      721 cggacatgta cacgggctgg ggcctgcgca cgctctcgtc gcgctacccg acgtacaacc
      781 cgatgagcta ccacaacgga tcggtgtggc cgcacgactc ggcgatcgcc gcgcacggca
      841 tgtcgcgcta tggcttccgc gaggaggcga acgaggtggt gagcggcctc atcgaggccg
      901 gacggcgctt cccgaacgcg cgcctgccgg agctgttctg cggattccag cgcgacctgc
      961 gcttctctgc ccggccggcc gactatcccg tgtcgtgcat cccgcaggca tggtccgcgg
     1021 gcgtggtgtt cctctgcctg cgcacactgc tgggcgcgga gcccgatctg cgcacgcagc
     1081 gcctgctgct cgatcccgcg ctgccgccgt ggctcgagcg gatcgacgtg cgcgacctgc
     1141 gcatgttcga cgcgccggtc tcgttccgcg tccggcgcca ccggaccggc gaccgcgtcg
     1201 ccggcggccg cggtcgagtc acgcgctcga gggtcacatc gccggcctga agcgcggcgc
     1261 acggcgcgtc ctgctcgtcc actacacgac accggccgtg ctcggcggcg tcgagcaggt
     1321 gatgggctac cacgcggccg gtctgcgcga cgcgggagcc acggtcacca tcctcgcggg
     1381 gcgcggcggg gcgccgcgcg gtgtcacgat cgcgcgcatc cgcgacgtcg actcgcgctc
     1441 ggccgccgtc gaacgcgatc gtatcgcgct cctgcggggc cgtcgcaccg cggagcatga
     1501 cgcgctcgtc gcccgcctcg tcgcgcgcat cggtccgctc gtcgcgaagg ccgaccgcgt
     1561 cgtcgtccac aacgtgatga cgatgcccat gaacctcgcc ctcaccgagg cactcgcgcg
     1621 ccttgcggcg gatcatcccg gccgcttcat cgcctggacg cacgacctcg cgcgctacga
     1681 cgcgcgctac gacggctacc gtcatcccgg tgagccgtgg gatctcgtcg agcgagcgtt
     1741 gccgggattc cgctacgtga ccgtctcgag cgagcgcgcg gcacagctcg ccgagctcac
     1801 gggtctcgcg cgcgagcgga tctccgtggt caccaacggc atcgacgtgc cgacggccat
     1861 cggcctcgcg ccggagagcc gcgacctctt ccgccgcttc cagttcgcgg acgccgaccc
     1921 gctcctgctg ctgcccgcgc gcgtgacgcc gcgcaagcgc atcgaggcgg cgatcgacgc
     1981 gacggccgcg ctgcgccgcc gcggccatcg cccgatgctc gtcgtcaccg gcggcgtcgg
     2041 cccgcacgac gcgcgcaacc gcgcgtacct cgcggccctg gtcgagcggg cgaagaacgt
     2101 ggagggcgtc gtcctgctcg ccgcgcacgg catcaccatc gactatcacg tcgtcgtgga
     2161 tctctacggc ctcgcggacg tgctggtgtt cccgagcgcg cacgagggct ttgggctccc
     2221 ggtcctcgag gccggcctgc accggcttcc gatcgtctgc agcgacatcc ccgcgctgcg
     2281 cgagatcggc ggcgacgagc cgcactacat cccgccc
//
LOCUS       DASTZR010000167         4447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1263190, whole genome shotgun sequence.
ACCESSION   DASTZR010000167 DASTZR010000000
VERSION     DASTZR010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263190"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1629
                     /locus_tag="VFV20_03725"
     CDS             <1..1629
                     /locus_tag="VFV20_03725"
                     /inference="COORDINATES: protein motif:HMM:NF019318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEU5287487.1"
                     /translation="ELDNTLRSLYGQGIRAEQLEPVRADAAKALPPAWTDRQKWLATE
                     IVRQFLEVNVTVDGTATAIAQQAARAAVTPVQVQVVAGEVVIRSGSVVSALDVEKLRA
                     LGLSSTSIDWQGALGLMLWAILIAGVVGLFAERYANEAWNDDRKLVLFALAILSLTVI
                     TRALVPGHTLLVYFVPYAAVAMTLAVLVSGRAALAAQIAGALHAGIMTGQIELVAYVL
                     VPALLGIAAVRRATTAREFAAGAVFVVAGNIGVLLVFLLIGRATDPLGAAQLLLAALA
                     SGAGSGILAFAAMAMLGHLFQITTVFELRELSDPNHPLLRQLLLRTPGTYHHSLLVAN
                     LAERAAEVIGADPLLARVGAYYHDIGKMRNPSAFIENQTGANPHDELDPLVSAGIVAA
                     HIRDGMALAERYHLPAVIREMIPGHHGTSVVRYFFQLAQQRGQDPDPAKYRYPGPRPR
                     TKECAIVMLADGTEASVRSLVEKNPDTIRAMVDKIVGERLEDGQLSDSDLTLRDVQRI
                     KDAFCELLLGVYHERIPYPEDRIARLPQRDAAAR"
     gene            1652..2131
                     /gene="ybeY"
                     /locus_tag="VFV20_03730"
     CDS             1652..2131
                     /gene="ybeY"
                     /locus_tag="VFV20_03730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU5287488.1"
                     /translation="MKIAVRGIVPCDLTAVRRAMHAAARPYGLPSGTVVTLAFVGDDE
                     MRALNRRYRRADRTTDVLSFGERVAFRDRGTAAARRLAADPDGVTRLGDVVISAARAA
                     RQARRRRKPLGAEIAFLAAHGVLHLLGFDDETAAGYREMVALGTAATRSTRPRPTKR"
     gene            2174..3862
                     /locus_tag="VFV20_03735"
     CDS             2174..3862
                     /locus_tag="VFV20_03735"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU5287489.1"
                     /translation="MTAHLLTAALVAGSLLAGSPARASTSELIRHLDELAGGFPGGVG
                     IYIADPRTPQPLYARNADEHVITASLYKLGVLAEAERRVDAGDLKYSDLISIEPEDIT
                     EDGSFEPVGTALTVDEALEKMITISDNGSALALWRVLGPANIDATLVKIGLRDFHVAL
                     DHDEDNVATPRAVGSYFAMLANRQLISAAASDRMLARLARQEINDRLPAALPDGVVVA
                     HKTGNLPGITHDAGIIFTPSGPRVVVAMTWDTYEEDAATFISNIGAAVYSAVLEPPAN
                     ARYAVPRTVVADIASRARVTVTVTNAGPNSWAASGAGAVGLIWELRDADDTMLLSSPA
                     ATRLPALAPSRSANIGLQIPTPADPGIFKVTIGLVDANGRALSGLGAATADFEIKTHK
                     PFLVKATIAVPMVLHRGEPSLVTTKFTALATAGTSGHDLVLTWRVLDTRNGRTVEDGI
                     EPVGRLQPGSEGTFFSSLAAPKILGTYRLAYELREGDDAVSETTTRTVTIVGPRTYPD
                     DEGGRTSGPFDGTPATTPRARPPFPTPSGAIVPRLDLPPFPSPKGKASPPPTPR"
     gene            3951..>4447
                     /locus_tag="VFV20_03740"
     CDS             3951..>4447
                     /locus_tag="VFV20_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5287490.1"
                     /translation="MRNHAATHIRVVVADDHPSIRENLRYLLNAEADMRVVGVASDGA
                     EALTVASDALPDVLVLDHDMPGLDGLAVMRRLRTDHPGIAVVMYTAQPEMCDDAMNAG
                     AAGCVGKDESPALLLDTIRNAAAGLALGDLRRNAAGALPHIEPLEVAALARAIEAGQI
                     EAYLQP"
BASE COUNT          654 a         1647 c         1520 g          626 t
ORIGIN      
        1 gagctcgaca acacgctgcg cagcctctac gggcagggca tccgcgccga gcagctcgag
       61 ccggtgcggg ccgacgcggc aaaggcgctt ccgccggcgt ggaccgatcg tcagaagtgg
      121 ctcgcgaccg agatcgtgcg ccagttcctc gaggtgaacg tgaccgtcga cggcacggcc
      181 acggcgatcg cgcagcaggc cgcacgcgcg gcggtgaccc cggtgcaggt gcaggtcgtc
      241 gccggcgagg tcgtcatccg gtcgggcagc gtcgtgtccg cgctggacgt ggagaagctg
      301 cgggcgctcg gcctctcgag cacgagcatc gactggcagg gcgcgctcgg cctgatgctg
      361 tgggcgatcc tcatcgcggg cgtcgtgggc ctcttcgccg agcgctacgc caacgaggcg
      421 tggaacgacg accgcaagct cgtgctcttc gcgctcgcca tcctctcgct gacggtgatc
      481 acgcgtgcgc tcgtgccggg ccacaccctg ctcgtgtact tcgtgccgta cgcggccgtc
      541 gcgatgacgc tcgccgtgct cgtctccggc cgcgccgcgc tcgccgcgca gatcgccggc
      601 gcgctgcacg cgggcatcat gaccgggcag atcgagctcg tggcctacgt gctcgtcccg
      661 gcgctcctcg gcatcgccgc ggtgcgacgt gccacgaccg cgcgcgagtt cgccgccggg
      721 gcggtattcg tggtggccgg gaacatcggt gtccttctcg tcttcctgct catcggccgg
      781 gccaccgatc cgctcggtgc cgcgcagctg ctcctcgcgg cgctggcgag cggtgcgggc
      841 tcgggcatcc tcgcgttcgc ggccatggcc atgctcggcc acctcttcca gatcacgacg
      901 gtgttcgagc tgcgcgagct gtcggatccg aaccatccgc tcctgcgcca gctgctgctg
      961 cggacgcccg gcacgtacca ccactcgctg ctcgtcgcca acctcgccga gcgcgcggcg
     1021 gaggtcatcg gcgcggaccc gctcctcgcg cgggtcggcg cgtactacca cgacatcggc
     1081 aagatgcgga atccgtcggc gttcatcgag aaccagaccg gcgcgaatcc gcacgatgag
     1141 ctcgatcccc tggtgtccgc cgggatcgtg gcggcgcaca tcagggacgg aatggccctt
     1201 gcggagcgct atcacctgcc ggcggtcatc cgcgagatga tcccgggcca ccacgggacg
     1261 agcgtggtgc ggtacttctt ccagctcgcg cagcagcgcg gccaggatcc cgatccggcg
     1321 aagtaccgct atccaggacc gcgcccgcgc acgaaggaat gcgcgatcgt catgctcgcg
     1381 gacggcaccg aggcctcggt ccgctcgctc gtcgagaaga accccgacac gatccgcgcg
     1441 atggtggaca agatcgtcgg cgagcgtctc gaggatggac agctctcgga cagcgacctc
     1501 acgctgcgcg acgtgcagcg catcaaggac gcgttctgcg agctcctgct cggcgtgtac
     1561 cacgaacgca tcccgtatcc cgaggatcgc atcgcgcgcc tgccacagcg cgacgccgcc
     1621 gcacgctaga cccggcgagc gacgaacgcg ggtgaagatc gcggtccgcg gcatcgtccc
     1681 ctgcgacctc acggcggtcc gccgcgcgat gcacgcggcc gcgcggccct acggcctccc
     1741 gagcgggacg gtggtgacgc tcgcgttcgt cggcgacgac gagatgcgcg cgctgaaccg
     1801 ccgctaccgt cgagccgacc ggaccacgga cgtgctctcg ttcggcgaac gcgtcgcctt
     1861 tcgcgaccgg ggcaccgccg ccgcgcggcg gctcgcggcc gatccggacg gtgtgacgcg
     1921 gctgggcgac gtggtgatca gcgcggcgcg ggccgcgcgc caggcccgcc gccgccggaa
     1981 gccgctgggc gcggagatcg cgttcctggc tgcccacggg gtgctgcatc tgctcggctt
     2041 cgacgacgag acggccgccg gctaccggga gatggtcgcc ctcggcacgg ccgcgaccag
     2101 atccacacgc ccccgtccta ccaaacggtg aaacgtcggc atctaattac cgttcaacat
     2161 gccgaatatg cgcttgacgg cccaccttct caccgcggcg ctcgtcgccg gctccctcct
     2221 cgcggggtcg cccgcgcgcg cgagcacgtc cgagctcatc cgtcatctcg acgagctggc
     2281 gggtggcttc cccggcggcg tcgggatcta catcgccgat ccacgcaccc cgcagccgct
     2341 ctacgcgcgg aacgcggacg agcacgtgat caccgcgtcc ctctacaagc tcggcgtcct
     2401 cgcggaggct gagcgtcgcg tcgacgcggg cgacctcaag tacagcgacc tcatcagcat
     2461 cgagcccgag gacatcaccg aggacggctc gttcgagccg gtcgggaccg cgctcaccgt
     2521 cgacgaagcg ctggagaaga tgatcaccat ctcggacaac gggagcgccc tggcgctctg
     2581 gcgtgtgctc ggccccgcga acatcgacgc cacgctcgtg aagatcgggc tcagggactt
     2641 ccacgtcgcg ctcgaccacg acgaggacaa cgtggcgacg ccacgcgcgg tcggcagcta
     2701 cttcgcgatg ctcgcgaacc ggcagctcat ctcggccgcc gcctccgacc gcatgctggc
     2761 gcgtctcgcg cggcaggaga tcaacgaccg cctgccggcg gcgctccctg atggcgtcgt
     2821 ggtggcgcac aagaccggca acctgccggg catcacgcac gatgccggga tcatcttcac
     2881 gccgtccggt ccgcgcgtcg tcgtcgcgat gacctgggac acctatgagg aggacgccgc
     2941 gacgttcatc tcgaacatcg gcgcggcggt gtacagcgcc gtcctcgagc cgcccgcgaa
     3001 cgcgcgttac gccgtgccac ggacggtcgt cgcggatatc gcgtcgcgcg cccgcgtgac
     3061 cgtgacggtg acgaacgccg gaccgaacag ctgggccgcg agcggcgccg gcgcggtcgg
     3121 gctcatctgg gagctgcgcg atgccgacga cacgatgctc ctctcatcgc ccgcggcgac
     3181 gcggctcccc gcgctcgcgc catcgagatc cgcgaacatc gggctgcaga tcccgacccc
     3241 ggccgatccg ggcatcttca aggtgacgat cggactggtc gacgccaacg gacgagcgct
     3301 ctccggtctc ggtgccgcga ccgcggactt cgagatcaag acgcacaagc cgttcctcgt
     3361 gaaggcgacg atcgcggttc cgatggtgct ccaccgcggc gagccgtcgc tcgtgacgac
     3421 gaagttcacc gcgctggcca cggccggcac gagcgggcac gatctggtgc tgacctggcg
     3481 cgtcctcgac acgcgcaacg gacgcaccgt cgaggacggg atcgagccgg tcggcaggct
     3541 gcagcccggg agtgagggca ccttcttctc gagcctcgcc gcgccgaaga tcctcgggac
     3601 ctaccggctc gcgtacgagc tgcgcgaggg cgacgacgcg gtgagcgaga cgaccacgcg
     3661 cacggtcacg atcgtgggac cgcgcaccta cccggacgac gagggcgggc gcaccagcgg
     3721 tccgttcgac ggcacgccgg caaccacccc acgcgctcgc ccgccgttcc cgacgccgtc
     3781 cggcgcgatc gtgccgcggc tcgatctgcc gccgttcccg tcgccaaaag gcaaggcgtc
     3841 gcctccgccg accccgcgct agccgcagcc gtctcacttc ggtctcgaca gctcggtcgc
     3901 ccgccttctg agcgttcggc catgagacct gcgtgacgta ggttaagcgc atgcggaatc
     3961 acgccgcgac gcacatacgc gtggtggtgg ccgacgacca tccgtccatc cgcgagaacc
     4021 tgcgctacct cctgaacgcc gaagcggaca tgcgcgtggt cggcgtggcg tcggacggcg
     4081 cggaggccct cacggtggcg tcggacgcgc ttccggacgt gctcgtcctc gaccacgaca
     4141 tgcccggcct cgacggcctc gcggtgatgc gccggctgcg caccgaccat cccgggatcg
     4201 ccgtcgtgat gtacacggcg cagccggaga tgtgcgacga cgccatgaac gcgggcgccg
     4261 ccggctgcgt gggcaaggac gagtcgccgg cgctcctcct cgacacgatc cgcaacgccg
     4321 cggccggcct ggcgctcggg gacctccgcc gcaacgcggc cggcgcgctg ccgcacatcg
     4381 agccgctgga ggtcgcggcc ctcgcgcgcg cgatcgaggc cggccagatc gaggcctacc
     4441 tgcagcc
//
LOCUS       DASTZR010000168         4289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_373148, whole genome shotgun sequence.
ACCESSION   DASTZR010000168 DASTZR010000000
VERSION     DASTZR010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373148"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /gene="tilS"
                     /locus_tag="VFV20_03745"
     CDS             <1..629
                     /gene="tilS"
                     /locus_tag="VFV20_03745"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEU5287491.1"
                     /translation="APRQRALADAWRAVTGKTLSARNRAALEALTRRTDGSRRLDLPG
                     GAALREYGELRLGRPVVATARDTVARLERGSSVTWHGWRISLDMRTRDTALSGRVDAT
                     SAGRLSVRARRAGDRLGRSRKLQDLFVDAKVPARLRDTWPIVALDDAVLWVPGLSPAP
                     TSGPIAISAERVGDGPTAGEDFPVKSAHEGQVASKAEARRQGGKRGRP"
     gene            626..2587
                     /gene="ftsH"
                     /locus_tag="VFV20_03750"
     CDS             626..2587
                     /gene="ftsH"
                     /locus_tag="VFV20_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU5287492.1"
                     /translation="MNLDNRIFKNGILTLLLVVMGLALLYAYRSQTPAMQIVPITQAV
                     QDIQAGKVKTVTEVGNKATLELSDGSKEQTQLSENSKTDQIAEAVAAYNAANPTRQVV
                     LKYDEPSATFSVIGSILLSLLPVLLIGGFFFYMMRQAQGTNNQALSFGKSRARMFIGN
                     KPTVTFDDVAGVDEAKQELTEVVEFLKYPEKFSTLGARIPRGVLLVGPPGTGKTMLAR
                     AVAGEAGVPFFSISGSEFVEMFVGVGASRVRDLFDQAKRNAPCIVFIDEIDAVGRQRG
                     AGLGGSHDEREQTLNQILVEMDGFDTGTNVIVLASTNRPDVLDPALLRPGRFDRQVVL
                     DRPDIKGRKAILEVHVRGKPLDKNVDLTRIGQISPGFSGADLANVVNEAAILAARRNK
                     KTIGQIDFEEALEKVALGPERRSRVITEKEKWIVAYHEAGHALCFKLLKHTNPVHKIS
                     IVARGMAMGVTWSLPQEDRYMRTKQEFEDDIAAALGGWVAEQLHLGGDATTGASNDIE
                     KASHMARQMVTKYGMSEKLGPLQYGKSDELIFLGRQIQEERNYSEEIAKIIDSEVHDI
                     VERGRQRAYEVLTSHRDKLDLVVSKLIEQESLESDEFNALFEDGDAGLPVAASSGPDA
                     TPPGAPAGEAKPGDKRERGKGPAAAPTPA"
     gene            2677..2823
                     /locus_tag="VFV20_03755"
     CDS             2677..2823
                     /locus_tag="VFV20_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287493.1"
                     /translation="MSKLVLATMDRIDHSESLQMALLTGASLVLGIVFLGLLIADEAH
                     AAVF"
     gene            2894..3460
                     /gene="thpR"
                     /locus_tag="VFV20_03760"
     CDS             2894..3460
                     /gene="thpR"
                     /locus_tag="VFV20_03760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEU5287494.1"
                     /translation="MRLFVGVPLPSDVAAAAFACLPDLPGVRRVRPELMHVTLAFLGS
                     VEDDRLPEVVAAVREACRGRARFEVSLESLGRFPAEGSPRIVWLGIVRGAPELIRLAD
                     GLRTAIARRELPFDAKPFRAHVTLARVGDRADHATTRALASLVARARTPALAFRAETV
                     VCFESVVSSKGPRYTPRATVSLEVGEER"
     gene            3457..3717
                     /locus_tag="VFV20_03765"
     CDS             3457..3717
                     /locus_tag="VFV20_03765"
                     /inference="COORDINATES: protein motif:HMM:NF038399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Os1348 family NHLP clan protein"
                     /protein_id="HEU5287495.1"
                     /translation="MSQRARDDILERAMSDAAFRALLARDPGAALAEYDLTPEERALF
                     HATTARAERLEDRISKTDLAAGMAVKTSSPLLKAPSEAAKKR"
     gene            complement(3953..4264)
                     /locus_tag="VFV20_03770"
     CDS             complement(3953..4264)
                     /locus_tag="VFV20_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5287496.1"
                     /translation="MTLSDEGYEVLTASHGADALTVVDHHMPSLILLDMRMPVMDGWA
                     VARALREAGSRVPIVVMTAAENARRWADEIGAAGHLAKPFALDELIGTVERYGSDERR
                     N"
BASE COUNT          671 a         1505 c         1492 g          621 t
ORIGIN      
        1 cggccccgcg gcagcgggcg ctcgccgacg cgtggcgcgc ggtgaccggg aagacgctga
       61 gcgcgcgcaa ccgcgcggcg ctcgaggcgc tcacgcgcag gacggacgga tcgcggcggc
      121 tcgatctgcc gggcggcgcg gcgctgcgcg agtacggcga gctccggctc ggccgcccgg
      181 tcgtcgcgac ggcgcgagac accgtggcgc ggctcgagcg cgggtcctcg gtgacgtggc
      241 acggttggcg catttcgctc gatatgcgaa caagagatac cgctctgagc ggccgcgtgg
      301 acgcgacgag cgcggggcgt ctctcggtgc gtgcgcgccg ggcgggggac cggctcgggc
      361 ggagccggaa gctgcaggac ctcttcgtcg acgcaaaggt cccggcgcgg ctgcgcgaca
      421 cctggccgat cgtcgcgttg gacgatgcag tgctgtgggt accaggactg tcgcccgcgc
      481 cgacaagcgg cccgatcgcg atcagcgcgg aacgggtggg ggacggccct accgcgggtg
      541 aggactttcc ggtgaagtcc gcgcacgagg gtcaggtagc atcgaaggca gaagcacgcc
      601 gacaaggcgg gaagcgaggc cgaccctgaa cctggataac cgcatcttca agaacggcat
      661 cctgacgctg ctgctggtcg tcatgggtct ggcgctcctg tacgcctacc ggtcccagac
      721 gccggcgatg cagatcgtgc cgatcacgca agccgtccag gacatccagg cggggaaggt
      781 gaagaccgtc acggaggtcg gcaacaaggc gacgctcgag ctgtccgacg ggagcaagga
      841 gcagacccag ctctcggaga acagcaagac cgatcagatc gcggaggccg tcgccgccta
      901 caacgccgcc aaccccacgc gccaggtcgt cctgaagtac gacgagccga gcgcgacgtt
      961 ctcggtcatc ggctcgatcc tcctgagcct gctgccggtg ctgctgatcg gtggcttctt
     1021 cttctacatg atgagacagg cacagggcac gaataaccag gccctgtcat ttggcaagag
     1081 ccgcgcgcgc atgttcatcg gcaacaagcc gacggtcacg ttcgacgacg tcgccggcgt
     1141 ggacgaggcg aagcaggagc tcaccgaggt ggtggagttc ctcaagtacc cggagaagtt
     1201 ctcgacgctc ggtgcgcgca tcccgcgcgg cgtgctcctg gtgggcccgc cgggtacggg
     1261 caagaccatg ctcgcgcgtg ccgtcgcggg tgaggccggc gtgccgttct tctcgatctc
     1321 gggctccgag ttcgtcgaga tgttcgtcgg cgtcggcgcg tcgcgcgtgc gcgacctgtt
     1381 cgaccaggcc aagcgcaacg cgccctgcat cgtgttcatc gacgagatcg acgcggtcgg
     1441 ccgccagcgc ggcgccggcc tcggcggctc gcacgacgag cgcgagcaga ccctgaacca
     1501 gatcctcgtc gagatggacg gcttcgacac cggcacgaac gtcatcgtgc tcgcctcgac
     1561 gaaccgcccc gacgtcctcg acccggccct cctccgtccc ggccgcttcg accgtcaggt
     1621 cgtgctcgac cgcccggaca tcaaggggcg caaggcgatc cttgaggtcc acgtccgcgg
     1681 caagccgctg gacaagaacg tcgacctcac gcgcatcggt cagatctcac ccggcttctc
     1741 gggcgcggac ctcgcgaacg tcgtgaacga ggccgcgatc ctcgccgcgc gccgcaacaa
     1801 gaagacgatc ggccagatcg acttcgagga ggcgctggag aaggtcgcgc tcggtcccga
     1861 gcgccgcagc cgcgtcatca ccgagaagga gaagtggatc gttgcctacc acgaggccgg
     1921 ccacgctctc tgcttcaagc tgctcaagca caccaacccg gtgcacaaga tctcgatcgt
     1981 cgcccgcggc atggccatgg gcgtgacctg gtcgctcccg caggaggacc gctacatgcg
     2041 gaccaagcag gagttcgagg acgacatcgc ggccgccctg ggcggctggg tcgccgaaca
     2101 gctccacctc ggcggcgatg ccacgaccgg cgcgtccaac gacatcgaga aggcgtcgca
     2161 catggctcgc cagatggtca cgaagtacgg catgagcgag aagctcggcc cgctccagta
     2221 cggcaagagc gacgagctca tcttcctggg ccgccagatc caggaggagc ggaactactc
     2281 ggaggagatc gccaagatca tcgactccga ggtccacgac atcgtcgagc gcggccgcca
     2341 gcgcgcgtac gaggtgctga cgagccaccg tgacaagctc gacctcgtcg tgagcaagct
     2401 catcgagcag gaatcgctcg agagcgacga gttcaacgcg ctcttcgagg acggcgacgc
     2461 cggtctgccc gtcgccgcgt cgagcggtcc cgacgcgacg ccgcccggcg cacccgccgg
     2521 tgaggcaaag ccgggcgaca agcgcgaacg cgggaagggt cctgccgcgg caccgacacc
     2581 cgcctaaccc gacgttcctg gcgactttag gccgttacaa gagcgttgtc cgcctctgta
     2641 acgaaaggca cgtcgcttgc ttaatggcat tgagcaatga gcaagctggt gctggccacg
     2701 atggaccgga tcgaccacag cgagtcgctg cagatggcgc tgctcaccgg cgcgtcgctc
     2761 gtcctgggta tcgtgttcct cggtctgctc atcgccgacg aagcgcacgc cgccgtcttc
     2821 tagcgccacg tagcaccgcg ccgggcggcg gcctcggctc cccgctcgcg ccggtggcat
     2881 agcctcgcgc gagatgcgcc tcttcgtcgg ggtcccactc ccatcagacg ttgccgcagc
     2941 ggcgttcgcg tgcctcccgg accttcccgg ggtgcgccgc gtccgcccgg agctcatgca
     3001 tgtgaccctg gcgttcctcg gcagcgtcga ggacgaccgt ctgccggagg tcgtcgccgc
     3061 ggtgcgcgag gcgtgccgcg gccgggcgcg gttcgaggtg tcgctcgaga gcctcggccg
     3121 cttcccggcg gaggggagtc cgcgcatcgt gtggctcggg atcgttcgcg gggcgcccga
     3181 gctcatccgc ctcgcggacg gtctccgtac ggcgatcgcg cgtcgcgagc tgcccttcga
     3241 cgcgaagccg ttccgcgcgc acgtcacgct cgcgagggtc ggcgaccgcg cggaccacgc
     3301 gacgacccgc gcgctggcgt ccctggtcgc ccgcgcgcgt acgccggcgc tcgcgttccg
     3361 cgcggagacc gtggtgtgct tcgagagcgt cgtgtcgtcc aaaggcccgc gatacactcc
     3421 gcgcgcgacg gtgtcgctcg aggtgggtga ggaacggtga gccagcgcgc gcgtgacgac
     3481 atccttgaac gtgccatgtc cgacgcggcc ttccgcgcgc tcctcgcccg cgaccccggg
     3541 gcggcgctcg cggagtacga cctcacgccg gaagagcgcg cgctcttcca cgcgacgacg
     3601 gcgcgcgcgg agcgtctgga ggaccgcatc tccaagacgg atctcgccgc cgggatggcc
     3661 gtgaagacgt cctcgccgct cctgaaggcg ccgtcggagg ccgcgaaaaa gcgctagctg
     3721 cggtctccgc agactcgggt cgcgtccgcg tggcggcggg tcctgtcccg ctccgggcgt
     3781 cgttctcccc tcgcgaagtc cggctccccg gcccgcccgt cggccggtcg cgtgatggac
     3841 cgcactgcta cgggcgtccg gggcccctcg gacttcgctt cggtgagagc gatcgccctg
     3901 cgcgtgccac ccgccgccac gcgggtgtga ccgaggtgtt aggagagcgc ggctagttcc
     3961 ggcgctcgtc cgaaccgtag cgctcgacgg tgccgatgag ctcgtcgagc gcgaatggct
     4021 tcgccaggtg gcccgcggcc ccgatctcat ccgcccagcg cctggcgttc tccgccgccg
     4081 tcatgaccac gatgggcacg cgcgagcccg cctcgcgcag cgcacgcgcg accgcccagc
     4141 catccatcac cggcatgcgc atgtcgagca ggatcagact gggcatgtgg tgatcgacga
     4201 ccgtcagcgc atcggccccg tgcgaggcgg tcaggacctc gtagccctcg tcggagagcg
     4261 tcatgtcgat cgactcgcgg atcccgtcg
//
LOCUS       DASTZR010000169         3134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_973116, whole genome shotgun sequence.
ACCESSION   DASTZR010000169 DASTZR010000000
VERSION     DASTZR010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973116"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VFV20_03775"
     CDS             <1..669
                     /locus_tag="VFV20_03775"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HEU5287497.1"
                     /translation="GDVTTDLTQSVPALPVDAAGLPIDGLQVEPGVVVVSVPLLPTAT
                     TRTVPVLWSLRGAVASGYWVSRVTTDPVAVTVSGDQKTLATLERIETVPVDVTGFTAS
                     RTIRAALALPDGVSLMEPTDATVTVTVVPLAGTRPFYVAIQILNLATGTSAETDPGSV
                     TVTLSGPQPALAGMATGQVVATVDAAGRAAGTYTVEVAVQVPSGTTAQSVQPARVTLT
                     IRAK"
     gene            698..2026
                     /gene="glmM"
                     /locus_tag="VFV20_03780"
     CDS             698..2026
                     /gene="glmM"
                     /locus_tag="VFV20_03780"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU5287498.1"
                     /translation="MSRLFGTDGVRGVANLDLTPELALRLGRAAGHVLGGRGHSVVIG
                     RDTRRSGRMLEGALAAGLCSVGIDVRLVGHIPTPGLAYLTRTKEFVAGAVISASHNPA
                     PDNGIKFFDHGGLKLPDAVEDAIEHAMADEASLPRPTEGGIGTVGDARGLVKDYEEFL
                     GTIAPPLGGMKLVLDCANGATYRVAPTVFAHAGAEVYTLFDTPDGENINDGCGATAPE
                     ALARAVVQRGADVGFAFDGDGDRVITVDANGRVHDGDVVLALAARHYARHGKLSPKLV
                     VGTIMSNGGLAATLARDGIELVRTKVGDRYVWEEMERRDAQFGGEPSGHVIFRGHHTT
                     GDGIFTALELLSLVHAEKRPLHELAGDLEHWPQVTRNVRAPRRAEWEESAAFAAAVRE
                     AETRLGESGRLVVRPSGTEPVLRITVEARDAQVASSTASRLEDIAKRELV"
     gene            2032..>3134
                     /gene="glmS"
                     /locus_tag="VFV20_03785"
     CDS             2032..>3134
                     /gene="glmS"
                     /locus_tag="VFV20_03785"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU5287499.1"
                     /translation="MCGIVGYVGPRTALPLLMDGLRRLEYRGYDSAGVALQKNGTLDV
                     LRAEGKLDNLASVVATHPSAGTTGIGHTRWATHGRPTEQNAHPHVDCRSATAVIHNGI
                     IENFQELKTPLVAAGHTFTSETDTEVVVHLLEDELKKGRTLEDAALAVLPHLEGAAAL
                     AFISTSDPGTIVAARISNAGGVIVGHGTNENFVASDMTALIEHTRDMSFLEAGDLAVV
                     TANGVHFRRIDDGSAVERPARKIDLDPIGAAKAGYKHFMLKEIFEQPRAISDTLLGRV
                     DRSSGNVVFDEDVKLTDAFIRSLPRITLLGCGTAGHAALVGKYLIERLAKIPCDVEIP
                     SEYRYRDPVVSKGQLAVAITQSGETADTLAAMHA"
BASE COUNT          477 a         1128 c         1115 g          414 t
ORIGIN      
        1 ggcgacgtga ccaccgatct cacccagagc gtgccggccc tgccggtcga cgcggccggc
       61 ctccccatcg acggcctgca ggtcgaaccc ggcgtggtcg tcgtcagcgt cccgctcctg
      121 cccaccgcga cgacgcgcac cgtcccggtg ctgtggtcgc tgcgcggcgc ggtcgcgagc
      181 ggctactggg tctcgcgggt cacgaccgat ccggtcgcgg tgaccgtgag cggcgatcag
      241 aagacgctcg ccacgctcga gcggatcgag acggtgccgg tggacgtcac cgggttcacc
      301 gcgtcgcgca cgatccgcgc ggcgctcgcc ttgcccgacg gcgtatcgct gatggagccc
      361 accgacgcga cggtcacggt cacggtggtg ccgctcgcgg gcacccgtcc cttctacgtg
      421 gcgatccaga tcctgaatct cgcgacgggc acgagcgcgg agaccgatcc cggcagcgtg
      481 accgtcaccc tctccggccc gcagccggcg cttgccggga tggcgaccgg acaggtcgtc
      541 gcgacggtgg atgccgcggg acgggcggcg ggcacgtaca ccgtggaggt cgcggtccag
      601 gtgccgtccg ggacgaccgc gcagagcgtg cagcccgctc gggtgaccct caccattaga
      661 gccaagtgaa cgtgagtcga gcgacgagcg gtaggacatg agccgcctgt tcggcaccga
      721 cggcgtgcgc ggcgtcgcca acctcgacct cacccccgag ctcgcgctgc gcctcggccg
      781 cgcggcaggc cacgtcctcg gtggccgcgg ccactccgtc gtgatcggcc gggacacgcg
      841 gcgcagcggc cgcatgctcg agggggcgct cgcggcgggc ctctgctcgg tcggcatcga
      901 cgtgcgcctg gtcggtcaca tcccgacccc tggtctcgcg tatctcacgc gcaccaagga
      961 gttcgtggcc ggcgccgtca tcagcgcctc gcacaatccc gcgccggaca acggcatcaa
     1021 gttcttcgac cacggcggcc tgaagctgcc cgacgccgtc gaggacgcca tcgagcacgc
     1081 gatggccgac gaggcctcgc tgccgcgacc caccgagggc ggcatcggca cggtcggaga
     1141 cgcgcgcggc ctggtgaagg actacgagga gttcctcggc accatcgcgc cgccgctggg
     1201 cggcatgaag ctggtgctcg actgcgcgaa cggtgcgacg taccgcgtcg cgccgacggt
     1261 cttcgcgcat gccggtgccg aggtgtacac gctcttcgac acaccggacg gcgagaacat
     1321 caacgacggc tgcggcgcga ccgcgccgga ggcgctcgcg cgcgcggtgg tgcagcgcgg
     1381 tgccgacgtc ggcttcgcgt tcgacggcga cggcgaccgc gtcatcaccg tggacgcgaa
     1441 cgggcgggtg cacgacggcg acgtcgtgct cgcgctcgcg gcacgccact acgcgcggca
     1501 cggcaagctc tcgcccaagc tcgtcgtcgg cacgatcatg tcgaacggcg gcctcgcggc
     1561 gaccctcgcg cgggacggga tcgagctcgt gcgcaccaag gtgggcgacc gctacgtgtg
     1621 ggaggagatg gagcgccgcg acgcgcagtt cggcggcgag ccctcgggcc acgtcatctt
     1681 ccgcggccac cacaccaccg gcgacggcat cttcaccgcg ctcgagctcc tctcgctcgt
     1741 gcacgccgag aagcgaccgc tgcacgagct ggcgggcgat ctcgagcact ggccgcaggt
     1801 gacgcgcaac gtgcgcgccc cgcgtcgcgc agaatgggaa gagagcgcgg cgttcgcggc
     1861 ggcggtgcgc gaggccgaga cgcgcctcgg cgagagcgga cgcctcgtcg tgcgcccgtc
     1921 ggggacggag ccggtgctcc ggatcaccgt cgaggcacgc gacgcgcagg tcgcgtcgag
     1981 caccgcgtcg cgcctcgagg acatcgcgaa gagggagctg gtctaaggag catgtgcggc
     2041 atcgtcgggt acgtcgggcc tcgcaccgcg ctcccgctcc tcatggacgg cctgcgccgc
     2101 ctcgagtacc gcggatatga ctcggcgggt gtcgcgctgc agaagaacgg cacgctcgac
     2161 gtgctccgcg ccgagggcaa gctcgacaac ctcgcgtccg tcgtcgcgac gcacccgtcc
     2221 gcggggacga ccgggatcgg tcacacgcgc tgggcgacgc acgggcgtcc gaccgagcag
     2281 aacgcgcatc cgcatgtcga ctgccgatcc gccaccgccg tcatccacaa cgggatcatc
     2341 gagaacttcc aggagttgaa gacgcccctc gtcgcggccg gccacacgtt cacctccgag
     2401 acggacaccg aggtcgtcgt ccacctcctc gaggacgagc tgaagaaggg gcggacgctc
     2461 gaggacgccg cgctcgcggt cctcccgcac ctcgagggcg cggccgcgct cgcgttcatc
     2521 tcgacgagtg atccgggcac gatcgtcgcg gcgcgcatca gcaacgcggg aggcgtgatc
     2581 gtcggccacg gcacgaacga gaacttcgtc gcctcggaca tgaccgcgct catcgagcac
     2641 acccgcgaca tgagcttcct ggaggccggc gacctcgcgg tggtgaccgc gaacggcgtg
     2701 cacttccggc gcatcgacga tgggtcggcc gtcgagcggc ccgcgcggaa gatcgacctc
     2761 gacccgatcg gcgcggcgaa ggccggctac aagcacttca tgctgaagga gatcttcgag
     2821 cagccgcgcg cgatctccga cacgctcctc ggacgcgtcg accggtcgag cgggaacgtc
     2881 gtgttcgacg aggacgtgaa gctgacggac gcgttcatca ggtcgctgcc gcgcatcacg
     2941 ctgctcggct gcggcacggc cggtcacgcc gcgctggtgg gcaagtacct catcgagcgg
     3001 ctcgcgaaga taccgtgtga cgtcgagatc ccgtcggaat accgctaccg cgatccggtc
     3061 gtgtcgaagg gccagctggc ggtcgcgatc acgcagtccg gcgagaccgc ggacacgctg
     3121 gccgcgatgc acgc
//
LOCUS       DASTZR010000170         4156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_897867, whole genome shotgun sequence.
ACCESSION   DASTZR010000170 DASTZR010000000
VERSION     DASTZR010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897867"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /gene="rpsB"
                     /locus_tag="VFV20_03790"
     CDS             complement(<1..320)
                     /gene="rpsB"
                     /locus_tag="VFV20_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013058850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU5287500.1"
                     /translation="MAVVTMKQLLESGVHFGHQARRWNPKMKRFIFMERNGIHIIDLQ
                     QTLSKVEDAYAFVRDLAGSGGQLLFVGTKKQAQESIAEEARRAGMHFVNQRWLGGFLT
                     NFVT"
     gene            501..887
                     /locus_tag="VFV20_03795"
     CDS             501..887
                     /locus_tag="VFV20_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287501.1"
                     /translation="MARYDDQKVPRGTSHRWEVLMSDEEHARPSAPRRTAIEVQAEHR
                     ILHEVPEQILREIPLVEEGASLVRRQEYLDFHDPARADFRAEGIEVVKPGQRIVSRAD
                     VSAEAWEELRAACDRVARRRPLRRAG"
     gene            complement(968..1120)
                     /locus_tag="VFV20_03800"
     CDS             complement(968..1120)
                     /locus_tag="VFV20_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEU5287502.1"
                     /translation="MIAFFREEEGQGLVEYALIIAVIAIAVIVAMIFLRGQLQNIFSN
                     IGNNLT"
     gene            complement(1255..1569)
                     /locus_tag="VFV20_03805"
     CDS             complement(1255..1569)
                     /locus_tag="VFV20_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287503.1"
                     /translation="MDARGRTRLDSWLASLSRERRASLYLLLDELCSGLDVSRHNRFG
                     FLRLRAEFETSGELFGCTLEELRAALAASLGDDLPERQRPPSALEELRSRIATRGHPD
                     FR"
     gene            complement(1586..>4156)
                     /locus_tag="VFV20_03810"
     CDS             complement(1586..>4156)
                     /locus_tag="VFV20_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HEU5287504.1"
                     /translation="RVRLERDGTLTAADRFATFVDPGIPVGPAITRLTGIRDVDLAGA
                     PSVEAALPNLAAFLGDAPIVGHNIAFDLAFLEHAGLSPHERLDTVELASILMPTAPTY
                     ALQALAAAADIHPGAAHRALDDALTCAAVLADLAERARALSPSLLAEAQAYAALLGGP
                     FESFFADASADTLRGAWDERRLAPPPRAAAAPAAGALAVSAVFAPDGPLARGLAGYEE
                     RAQQRDLARAIESTFADGGVLVAEAGTGVGKSLAYLVPALSRALGGERVVVSTHTLPL
                     QDQLVRKDLPALQEALGTRVPVTVLKGRSNYLCPRRWQQFRGAVATREEARLLLKTLV
                     WRAQTASGDRAELNLLGAESDLWPRISADDETCDARRCARVSGGCYLQRAREAAANAG
                     IVVTNHALLLQDARMRGALLPASDHLVVDEAHRLEEVATDAFGLTLEDRLVRRALDRV
                     SRAPAVTAGLRDDRAVDAETVRREVATAHERAAELFTGLAAVVGGGQEERLRVTGGLR
                     ASDERWLPVEIAGERLADAVGGVATASDRLAARTTDEDEQAELASAVAELTGIRIAIS
                     RGIHAPRPGDIVWLDRPFDAPVALRVAPSHLGDAIRRGLVERRRSAVFTSATLAVAGS
                     FAFALDRFGVGDVADAHAFGSPFDYAKQAILVVPDEGALPGEPDHADETAETVAEIGR
                     ALEGRTLVLFTAHGAMRDVASRLAGLEESGIAVLTQGIDGSRRALLERFAAGRAVLLG
                     TQSFWEGIDLPGDILQCVVITRLPFAVPDDPLVQGRSERYEDPFREFHLPQAALRLRQ
                     GFGRLIRTRSDRGAVVLLDRRALVREYGPELLAALPDARVRRVPREAIAMTVAQFCGR
                     "
BASE COUNT          634 a         1471 c         1507 g          544 t
ORIGIN      
        1 atggtgacga aattcgtgag gaagccgccc agccagcgct ggttcacgaa gtgcatcccg
       61 gcgcgcctgg cctcctcggc gatcgactcc tgcgcctgct tcttggtgcc cacgaacagc
      121 agctggccgc cgctgcccgc gaggtcgcgc acgaacgcgt aggcgtcctc gaccttggag
      181 agggtctgct ggaggtcgat gatgtggatc ccgttgcgct ccatgaagat gaagcgcttc
      241 atcttcgggt tccaacggcg tgcctggtgt ccgaaatgga cgcccgactc caggagctgc
      301 ttcatggtga cgactgccac gtgtctctcc cttgtattcc tccgcctccg tctcgcggtc
      361 cgcgtcgcct gggccaggcg gcctccggcg agggaacgga ccgatcggga aggcgtgcgt
      421 gatatcgggc gttccgcccg atccgagaac aagtgtagca agaggccacc tggcaccgcg
      481 cgtgccgcga acgcgctccg atggcgcgat acgacgacca gaaggtgccg cgcggcacct
      541 cccaccgctg ggaggtgctc atgtccgacg aggagcacgc gcggccgagc gcgccgcgac
      601 ggacggcgat cgaggtccag gccgagcatc gcatcctcca cgaggtcccc gagcagatcc
      661 tgcgcgagat ccccctcgtc gaggagggcg cgagcctggt gcgccggcag gaatacctcg
      721 acttccacga ccccgcacgc gccgacttcc gtgccgaggg catcgaggtc gtgaagcccg
      781 gtcagcggat cgtgagccgc gccgacgtga gcgcggaggc ctgggaggag ctccgggccg
      841 cctgcgaccg ggtggcgcgc aggcgtcccc tccggcgcgc aggctgaaca gaaacggaac
      901 gggcggcgca aaacgaggga cccggccgtg tggccgggtc cctcgctgaa ctgcgacgtc
      961 gcggttacta ggtcaggttg ttgccgatgt tgctgaagat gttctgcagc tggccgcgga
     1021 ggaagatcat ggccacgatg acggcaatgg cgataacagc gatgatgagg gcgtactcga
     1081 cgaggccctg accctcttcc tcacggaaga acgcgatcat accgaacctc cttcttcggg
     1141 tgctcgacag ccgaccagac ggctgccacc ggcaaaaagt ctaggcgcgc ttgaccagtc
     1201 ggcaataccc cgacggtagg gggcgcctgg tcctgatacg agactgcatt ccgcctagcg
     1261 gaagtcgggg tggccccgtg tcgcgatccg cgaccgcaat tcctcgagcg cacttggcgg
     1321 acgctgccgc tcggggaggt cgtctccgag cgacgccgcg agcgcggccc ggagctcctc
     1381 gagagtgcac ccgaagagct cgccggaggt ctcgaactcc gcgcgcagcc tgaggaagcc
     1441 gaaacggttg tgacgcgaga cgtcgaggcc gctgcagagc tcgtcgagca gcaggtagag
     1501 gctcgcgcgc cgctcccgtg aaaggctcgc gagccaggag tcgaggcggg tccggccccg
     1561 tgcgtccacg gggtgattct gctctctagc gtccacagaa ctgcgccacg gtcatcgcga
     1621 tggcttcccg gggcacgcgg cgtacccggg cgtccggcag cgcggcgagg agctccggcc
     1681 cgtattcgcg cacgagcgcc cgccggtcga gcagcacgac ggcgccacgg tcgctccgcg
     1741 ttcggatgag ccgcccgaag ccctggcgca gtcgcagcgc cgcctgcggc aggtggaact
     1801 cgcggaaggg gtcctcgtag cgctccgagc gaccctgcac cagcggatcg tcgggtacgg
     1861 cgaacggcag ccgcgtgatc acgacgcact gcaggatgtc gccgggcagg tcgatgccct
     1921 cccagaacga ctgcgttccc aggaggacgg cgcgtcccgc ggcgaagcgc tcgagcagcg
     1981 cgcgccgcga gccgtcgatc ccctgcgtga gcaccgcgat ccccgattcc tcgaggcccg
     2041 ccaggcgcga cgcgacgtcg cgcatcgcgc cgtgagcggt gaacaggacg agcgtgcggc
     2101 cctcgagcgc gcgtccgatc tccgcgaccg tctcggcggt ctcgtccgcg tggtcgggtt
     2161 cgccgggcag cgcaccctcg tcggggacga cgaggatcgc ctgcttcgcg tagtcgaacg
     2221 gcgagccgaa ggcgtgcgcg tcggcgacgt cgccgacgcc gaagcgatcg agcgcgaagg
     2281 cgaacgatcc cgcgaccgcc agcgtggcgg aggtgaacac ggccgagcgc cgacgttcga
     2341 ccagcccgcg ccggatcgcg tccccgagat gcgagggcgc gacgcgcagc gcgaccggcg
     2401 cgtcgaacgg gcggtcgagc cagacgatgt cgccgggacg cggggcgtgg atgccgcggg
     2461 agatggcgat gcgaatgccg gtgagctccg ccacggcgga tgcgagctcg gcctgctcgt
     2521 cctcatcggt cgtgcgcgcc gcaagacgat ccgacgccgt ggcgacgccg ccgaccgcgt
     2581 ccgccaggcg ctcaccggcg atctcgaccg gcagccaccg ctcgtcgctc gcgcgcagtc
     2641 cgccggtcac gcgcagacgc tcctcctgcc cgccgcccac cacggccgca agtcccgtga
     2701 agagctcggc ggcgcgctca tgtgccgtgg cgacctcgcg gcgcacggtc tcggcgtcga
     2761 cggcgcggtc gtcgcggagg ccagccgtca cggcgggcgc gcgcgacacg cggtccagcg
     2821 cgcggcgcac cagccggtct tcgagcgtga ggccgaacgc gtcggtcgcg acctcctcga
     2881 gacggtgtgc ctcatcgacg acgagatggt ccgacgcggg gagcaacgca ccgcgcatgc
     2941 gcgcgtcctg caacagcagc gcgtggttcg tcacgacgat gccggcgttc gcggccgcct
     3001 cgcgcgcgcg ctgcagatag cagccgccac tcacgcgcgc gcagcgtcgg gcatcgcacg
     3061 tctcgtcgtc ggccgagatg cgcggccaga ggtccgactc ggcaccgagg agattcagct
     3121 ccgcgcgatc gccgctcgcg gtctgggcac gccacacgag cgtcttcagg agaagccgcg
     3181 cctcctcgcg tgtcgcgacg gcgccgcgga actgctgcca gcgccgcgga cagaggtagt
     3241 tcgaccttcc cttcaacacg gtgaccggca cgcgcgtccc gagcgcctcc tgcagggccg
     3301 gcaggtcctt ccgcacgagc tggtcctgca gcggcagcgt gtgcgtgctc acgaccacgc
     3361 gctccccgcc gagcgcgcgc gacagcgcgg gcacgagata cgcgagcgac ttccccacgc
     3421 cggtcccggc ttcggccacg agcacgccgc cgtccgcgaa ggtgctctcg atcgcgcgcg
     3481 cgagatcgcg ctgctgcgcc cgctcctcgt agccggcgag cccgcgggcg agcggaccat
     3541 ccggagcgaa gaccgccgag accgcgagcg ctcccgcggc cggtgcggcc gcagcgcgcg
     3601 gcggcggtgc gagccggcgc tcgtcccatg cgccacgcag ggtgtcggcg ctcgcgtcgg
     3661 cgaagaagct ctcgaacgga ccgccgagga gcgcggcgta ggcctgcgcc tccgcgagca
     3721 gcgatggcga gagcgcacgc gcccgttcgg caagatccgc caggacggcc gcgcacgtga
     3781 gcgcgtcgtc aagggcgcga tgcgccgcgc ccggatggat gtcggccgcc gcggcgagcg
     3841 cctgcagcgc gtaggtgggt gcggtcggca tgaggatcga cgcgagctcg accgtgtcaa
     3901 gacgctcgtg cggcgacagc ccggcgtgct ccaggaaggc caggtcgaat gcgatgttgt
     3961 ggccgacgat cggcgcgtcg ccgaggaagg ccgcgagatt cggcagtgcc gcctccacgc
     4021 tcggcgcgcc cgcgaggtca acgtcgcgaa tgccggtgag gcgcgtgatc gccggcccga
     4081 ccgggatgcc gggatcgacg aaggtcgcga agcggtcggc agcggtgagc gtcccgtcgc
     4141 gctcgagacg cacgcg
//
LOCUS       DASTZR010000171         3514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1080344, whole genome shotgun sequence.
ACCESSION   DASTZR010000171 DASTZR010000000
VERSION     DASTZR010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080344"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VFV20_03815"
     CDS             <1..203
                     /locus_tag="VFV20_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287505.1"
                     /translation="NTYVWDGLRTAFPTALGQGQTATVNLAIRLPSTAGTFEIRVDLV
                     QEGVTWFTGQRIAPASISLQVQ"
     gene            200..1327
                     /locus_tag="VFV20_03820"
     CDS             200..1327
                     /locus_tag="VFV20_03820"
                     /inference="COORDINATES: protein motif:HMM:NF025330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEU5287506.1"
                     /translation="MTRIASVLALALVIVGLAWMDAPTVARGDAVRWSSFAELQRPRA
                     FASAIGLPSGEILVLGGLDPRDADVTNPRTELIDQFSGSVALLPQTILARLLQTVTLG
                     AGERVVVAGGVVWEGPRWAPVARVDVYEVARRSWRAAAPLHSARSDAAAAALKDGRVA
                     VFGGNFGTRLLDTVEIYDPRRDAWTEGAPMPRPRTQHSAVLLKDGRVLIAGGIDKDGG
                     PTATTFLYDPSANAWTAGPLMTQPRMQQSTVLLANGDVLFAGGDDAAASTSELFLASE
                     GRFVASGALQHPRFVAQAAALPDGRVVLNGGLPPHMTTYAPLGSTEIWDPRTGKWREV
                     APAASPRAWAKLISIEDAVFLVSGSGPGEDAVSSVERLTLD"
     gene            1459..3276
                     /locus_tag="VFV20_03825"
     CDS             1459..3276
                     /locus_tag="VFV20_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287507.1"
                     /translation="MKHLVRVLALAALVVSLVAQSTPASANDKIYGFVTDKTTGQVLA
                     DVCITLGPPIVCFTHTDAGGYFEIDLTALGATPGSQWELYFLKSGYLQGHLGPFFVNG
                     PTLASFAMTPGTTPPPGPTCPAQRAGLPTQTVYLPNITRRLGGAAGWYTPFIVQNTGL
                     ANTDLEVSFYKFSDGSCVSRLTVSALKPGTSYSNDPNDNGKNPGLPDDSQFSVVVRSF
                     GSTVVGIVNEHQGSGSAAEAMSYDGFTDGATTVYLPNITRRFFGFVTPFIIQNLGTAT
                     TSAVATFRPFDGSSGPITVTRSIDAGRAKPVDPNSNDMTLGAPGLVDGKQYSVTVTAS
                     QPIAVVVNTQNDAPGTATPMAASTDGVSAGAQVAYGSYAAKNASGRRTPIIVQNLGTA
                     AITPTLSFTPLAGSTGTATTYTFPTIQPNASKAFDPRFSFDSQKTSSPALCSGASANC
                     LGDGEYAFKVDGGTGALLAVQVNPESATTLMAYAGSAAPAAKYFLPNVTKSLCFCPTP
                     VNGVGWTTPLLIQSVTATSATLKWYRFSDGTLVHTQTISLTPGSGVRIDPWSVSQLPA
                     DAQYSVVADAGTTGTITAIVTEFASGGDNAMAYEGFAAP"
     gene            3273..>3514
                     /locus_tag="VFV20_03830"
     CDS             3273..>3514
                     /locus_tag="VFV20_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287508.1"
                     /translation="MTRVRLTRAQLIGIGAALAAVIAAGAAFAVGLPGGTAPTAQSSP
                     SQTQTSAPPGVPTPRSTPPGTPPPTPAEVTVSSAEIS"
BASE COUNT          514 a         1309 c         1181 g          510 t
ORIGIN      
        1 ggaacacgta cgtgtgggac ggtctgcgca ccgccttccc gaccgcgctc ggccagggcc
       61 agacggccac ggtgaatctc gcgatcaggc tgccctccac cgcgggcacc ttcgagatcc
      121 gcgtcgacct cgtgcaggag ggtgtgacgt ggttcacggg tcagaggatc gcgccggcca
      181 gcattagtct tcaggtgcag tgacccgcat cgcctccgtc ctcgcgctcg ccctggtcat
      241 cgtcggactc gcctggatgg acgcccccac cgtcgcgcgc ggcgacgccg tccgctggtc
      301 ctcgttcgcc gaattgcagc ggccgcgcgc gttcgcgtcc gcgatcggtc tgccgagcgg
      361 cgagatcctc gtgctcggtg gcctcgatcc gcgcgatgcg gacgtgacca atccgcgcac
      421 cgagctcatc gatcagttct ccggcagcgt ggccctgctg ccgcagacga tcctcgcgcg
      481 cctgctccag acggtgacgc tcggggcggg cgagcgggtc gtcgtcgcgg gcggcgtcgt
      541 gtgggagggc ccgcggtggg cgccggtggc gcgcgtcgat gtgtacgagg tcgcgcggcg
      601 cagctggcgc gccgcagcgc cgctccacag cgcgcgttcg gacgccgcgg ccgcggcgct
      661 gaaggacggc cgcgtcgccg tcttcggcgg caacttcggc acgcggcttc tcgacaccgt
      721 cgagatctac gacccgcgcc gcgacgcctg gaccgagggc gcgccgatgc cccggccgcg
      781 gacgcagcac agcgcggtgc tgctgaagga cggacgggtg ctcatcgcgg gcggcatcga
      841 caaggacggc ggccccacgg cgacgacgtt cctctacgac ccgtcggcga acgcgtggac
      901 cgccggaccg ctgatgaccc agccgcggat gcagcaatcg accgtgctgc tcgcgaacgg
      961 cgacgtgctg ttcgcgggcg gcgacgacgc ggccgcctcg acgtcggagc tcttcctcgc
     1021 gagcgagggc cgcttcgtcg cgagcggcgc gctgcagcat ccgcgcttcg tcgcgcaggc
     1081 cgcggcgctg cccgacggac gcgtcgtgct gaacggcggg ctgccgccgc acatgacgac
     1141 gtacgcgccg ctcggttcca ccgagatctg ggacccgcgc accggcaagt ggcgcgaggt
     1201 cgcgccggcc gcgtcgccgc gcgcgtgggc gaagctgatc tcgatcgagg acgccgtctt
     1261 cctcgtgagc ggcagcggac cgggcgagga tgccgtcagc tcagtggagc gcctgacgct
     1321 cgactgagcg ttcttcgcct tgtacgcacg aaaaacacat gtcgtctttt ggagcctcgt
     1381 ggacgtacag ttatcgaggc tcgtcgcgtt agacgcccta gaccgacgtt ctgtcggctt
     1441 ttcgttgagg aggagttggt gaaacacctc gtgcgtgtgc tcgctctggc ggccctcgtc
     1501 gtcagcctgg tcgcgcagtc gaccccggcg tcggcgaacg acaagatcta cgggttcgtg
     1561 acggacaaga cgaccgggca ggtcctggcg gacgtctgca tcacgctcgg tccgcccatc
     1621 gtctgcttca cgcacaccga cgccggcggc tacttcgaga tcgacctcac cgcgctcggc
     1681 gcgaccccgg gcagccagtg ggagctctac ttcctgaaga gcggctacct gcagggtcac
     1741 ctcggcccgt tcttcgtgaa cggtccgacg ctcgcgagct tcgcgatgac gccgggcacg
     1801 acgcctccgc cgggcccgac gtgccccgcg cagcgcgccg gcctgccgac gcagaccgtc
     1861 tacctgccga acatcacccg ccgcctcggc ggcgcggccg gctggtacac gccgttcatc
     1921 gtccagaaca ccggtctggc gaacaccgac ctcgaggtct ccttctataa gttctccgac
     1981 ggctcgtgcg tctcgcgcct caccgtgagc gcgctgaagc ccggcacctc ctactcgaac
     2041 gacccgaacg acaacggcaa gaacccgggc ctgcccgatg actcgcagtt ctcggtcgtc
     2101 gtgcgcagct tcggatcgac cgtcgtcggc atcgtgaacg agcaccaggg ctcggggagc
     2161 gcggccgagg cgatgtccta cgacggcttc accgacggcg cgaccaccgt gtacctgccg
     2221 aacatcaccc gccgcttctt cggcttcgtg acgccgttca tcatccagaa cctcggcacc
     2281 gcgacgacga gcgcggtcgc gacgttccgt ccgttcgacg gcagcagcgg cccgatcacc
     2341 gtcacgcgca gcatcgacgc cggccgcgcg aagccggtcg acccgaactc caacgacatg
     2401 acgctcggcg cgccgggcct cgtggacggc aagcagtact ccgtcaccgt gaccgcgtcg
     2461 cagccgatcg cggtcgtggt gaacacgcag aacgacgcac cgggcaccgc gacgccgatg
     2521 gccgcgtcca ccgacggcgt gagcgcgggc gcccaggtcg cctacggctc gtacgccgcg
     2581 aagaacgcga gcggcaggcg cacgccgatc atcgtgcaga acctcggcac cgcggccatc
     2641 acgccgacgc tcagcttcac gccgctcgct ggctcgacgg gcacggcgac gacgtacacg
     2701 ttcccgacga tccagccgaa cgcgtcgaag gcgttcgacc cgcggttctc gttcgactcg
     2761 cagaagacct cgtcgccggc actgtgcagc ggcgcgtccg cgaactgcct cggtgacggc
     2821 gagtacgcgt tcaaggtgga cggtgggacg ggcgccctcc tcgccgtcca ggtcaacccc
     2881 gagagcgcga cgacgctcat ggcctacgcc ggctccgccg cgcccgcggc gaagtacttc
     2941 ctgcccaacg tgaccaagtc gctctgcttc tgcccgacgc cggtgaacgg cgtcggctgg
     3001 acgaccccgc tgctcatcca gagcgtcacc gcgacgagcg cgacgctcaa gtggtaccgc
     3061 ttcagcgacg gaacgctcgt gcacacgcag acgatcagcc tgacgcccgg ctccggcgtg
     3121 cgcatcgacc cgtggtcggt gtcgcagctg cccgccgacg cgcagtactc ggtcgtcgcg
     3181 gacgcgggca cgaccggcac gatcaccgcg atcgtgaccg agttcgcatc cggtggtgac
     3241 aacgccatgg catacgaggg cttcgcggct ccgtgacgcg cgttcgcctg acgcgcgccc
     3301 aactcatcgg catcggcgcg gcgctcgcgg cagtcatcgc cgcgggcgcc gcgttcgcgg
     3361 tgggcctccc cggaggcacg gcgcccaccg cgcaaagctc cccatcgcag acgcagacgt
     3421 ccgcgccgcc gggcgtcccg accccgcgct cgaccccgcc cggaacgcct ccgcccaccc
     3481 cggcggaggt caccgtgagc tcggccgaga tctc
//
LOCUS       DASTZR010000172         2775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_140820, whole genome shotgun sequence.
ACCESSION   DASTZR010000172 DASTZR010000000
VERSION     DASTZR010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140820"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VFV20_03835"
     CDS             <1..338
                     /locus_tag="VFV20_03835"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEU5287509.1"
                     /translation="HVCEVDFSRSAVLLGPGRYLNHSCEPNAMRSGVKVFAWRRIRAG
                     EEITIDYRLNAFGGQRSRCRCGHCAGWLVNSFFALPRARQRAYLPYAPAFIRREFRRR
                     VAEANARSM"
     gene            complement(269..799)
                     /locus_tag="VFV20_03840"
     CDS             complement(269..799)
                     /locus_tag="VFV20_03840"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5287510.1"
                     /translation="MSTRVASRFTIEQAVEMLDTQPRRIAVATKGVSATRLRRDKVLG
                     EWTAVDILAHLRACSDALGDVIPDIVRGGEPLRAVNPRSHIRRTNYRELEFATSFRAF
                     ARQRARLLKYLRALPRRDWARSRLFTGMGTPRVRTVMFYADWLAGHERAHVRHIERAF
                     ASATRRRNSRRMKAGA"
     gene            complement(867..1655)
                     /locus_tag="VFV20_03845"
     CDS             complement(867..1655)
                     /locus_tag="VFV20_03845"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEU5287511.1"
                     /translation="MLRYLRVFRLFAISEFQFEAEYRWNFLLVLFEISLVVGTSVAAV
                     LVMFGHTTTLNGWTLPQMLVLLGVYYLVQGASNLVFEPSFERLMEHVRVGTLDFHLLK
                     PLNTQFLVSTRHMQMARIPDFLLGFLILAIGLAQMGETATPASALLFALALLCGLALV
                     YSLLLVLVTLSFWFVRVDNLLTIFWSFLDAGRFPVDVYPAWLRVTLSTVVPIGIAVTA
                     PSQAIASKIGAPEVALLAAGTVVAFVVASAFWRLGLRNYTGASA"
     gene            complement(1655..2449)
                     /locus_tag="VFV20_03850"
     CDS             complement(1655..2449)
                     /locus_tag="VFV20_03850"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEU5287512.1"
                     /translation="MIRIYRQLLTASFQGAAQYRIQFVLWMLFSVIRPTIFMAAWVAV
                     ANAQGGAVGGYSASSFAAYYVALALVTHLTGAWDFFEFEFEVRQGRLAPKLLRPLHPL
                     HYAIVQAVVFKLTTLPVLVVVLALLAVTFEARFDATPLHYVLFVPSVILGWLLNFMLG
                     WVVACAAFWLNRVHTVSTIMQRISFLFAGQIAPIALMPAALQVVCWALPFAYTLAVPT
                     MILRGDVGIELALILIVAQVAWLAVAFAVFQIVWRMGLRQFSAVGA"
     gene            complement(2446..>2775)
                     /locus_tag="VFV20_03855"
     CDS             complement(2446..>2775)
                     /locus_tag="VFV20_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEU5287513.1"
                     /translation="RRVIVIHHGVLLYDGALADLAERMAPYKRISVTLHTSDPRRDLS
                     AYGEVAVRDDYSATLHVPRREAPERTARLVRDLGDDLADISVEDPPIEEVIDRVFAAE
                     RPLAVAP"
BASE COUNT          487 a          946 c          980 g          362 t
ORIGIN      
        1 ggcacgtctg cgaggtcgac ttctcacgga gcgccgtcct ccttgggccg ggccgctacc
       61 tcaaccacag ctgcgagccg aacgcgatgc ggagcggcgt caaggtcttc gcgtggcggc
      121 gcatccgcgc cggcgaggag atcaccatcg attaccgcct caacgccttc ggcggccagc
      181 gcagccggtg ccggtgcggc cattgcgccg gctggctcgt caacagcttc ttcgcgctgc
      241 cgcgggcacg gcagcgtgcg tatctccctt acgcgcccgc cttcatccgg cgcgagttcc
      301 gtcgccgcgt cgccgaagcg aacgcgcgct cgatgtgacg cacgtgggcg cgctcgtgcc
      361 ccgcgagcca gtccgcgtag aacatcacgg tgcgcacgcg cggcgtgccc ataccggtga
      421 acagcctgct gcgcgcccag tcccggcgcg ggagggccct caggtacttc agcagcctgg
      481 cgcgctgccg cgcgaacgcg cggaacgacg tcgcgaactc cagctcgcga tagttcgttc
      541 ggcggatgtg cgaccgcggg ttcaccgccc gcagcggctc cccgccacga acgatgtcgg
      601 ggatcacgtc gcccagtgcg tccgagcagg cgcgcaggtg cgcgaggatg tccaccgccg
      661 tccactcgcc gagcaccttg tccctgcgca gccgcgtggc ggacacgccc ttcgtcgcca
      721 cggcgatgcg gcgcggttgc gtgtcgagca tctcgaccgc ctgctcgatg gtgaagcgcg
      781 aggcgacccg cgtgctcacg cacttgctcc ggtgtagtcc acgtccggtc gctccgctcc
      841 cgtccgcgtc tcatctgcgc gcgtgctcac gcacttgctc cggtgtagtt gcggaggccg
      901 agccgccaga aggcgctcgc gacgacgaac gcgacgacgg tccccgcggc gaggagggcc
      961 acctcgggcg ctccgatctt cgacgcgatc gcctgggacg gcgcggtcac cgcgatcccg
     1021 atcggcacga ccgtcgagag cgtcacgcgg agccacgcgg ggtacacgtc gaccgggaat
     1081 cggccggcgt cgaggaacga ccagaagatc gtgagcaggt tgtcgacgcg tacgaaccag
     1141 aacgacagcg tgacgagcac gaggagcagc gagtacacga gcgccaggcc gcagagcagc
     1201 gccagcgcga agagcagcgc gctcgccggc gtggccgtct cccccatctg cgcgagcccg
     1261 atggcgagga tgaggaaccc caggaggaag tccgggatgc gggccatctg catgtgccgc
     1321 gtcgacacga ggaactgcgt gttgagcggc ttcagaagat ggaagtcgag cgtgccgacg
     1381 cgcacgtgct ccatcagccg ctcgaacgac ggctcgaaca cgagattgct cgcgccctgc
     1441 acgaggtagt acacgccgag gagcaccagc atctggggga gcgtccaccc gttcagcgtc
     1501 gtcgtgtggc cgaacatcac gagcaccgcc gccacgctcg tgccgacgac gagcgagatc
     1561 tcgaacagga cgagcaggaa gttccagcgg tactccgcct cgaactggaa ctcgctgatc
     1621 gcgaagagcc ggaagacgcg caggtagcgg agcactacgc gccgaccgcg ctgaactggc
     1681 ggagacccat ccgccacacg atctggaaga cggcgaacgc gaccgcgagc catgcgacct
     1741 gcgccacgat gaggatgagc gcgagctcga tcccgacgtc gccgcgcagg atcatcgtcg
     1801 gcacggcgag cgtgtacgcg aacggcagcg cccagcacac gacctggagc gccgccggca
     1861 tgagcgcgat cggcgcgatc tgccccgcga acaggaatga gatccgctgc atgatcgtcg
     1921 agaccgtgtg aacgcggttc agccagaacg ccgcgcacgc gacgacccag ccgagcatga
     1981 agttcagcag ccagcccagg atcacgctcg gcacgaagag aacgtagtgc agcggggtcg
     2041 cgtcgaagcg cgcctcgaag gtgacggcga gcagcgcgag caccacgacg agcacgggga
     2101 gcgtcgtgag cttgaacacg acggcctgca cgatcgcgta gtgcagcgga tgcagcgggc
     2161 ggagcagctt cggtgcgagc cggccctggc gtacctcgaa ttcgaactcg aagaagtccc
     2221 acgcgccggt gaggtgggtc acgagcgcga gcgcgacgta gtacgcggcg aacgaggacg
     2281 cgctgtaacc gccgaccgcg ccgccctgcg cgttcgcgac cgcgacccac gcggccatga
     2341 agatggtggg ccggatgacc gagaagagca tccacagcac gaactggatg cggtactgcg
     2401 ccgcgccctg gaacgaggcg gtgaggagct ggcgatagat gcggatcacg gcgccaccgc
     2461 gagcggacgc tccgccgcga agacgcggtc gatgacctcc tcgataggcg ggtcctcgac
     2521 gctgatgtcg gcgaggtcgt cgccgaggtc acgcaccagg cgcgcggtgc gctcgggcgc
     2581 ctcgcgtcga gggacgtgca gcgtcgcgga gtagtcgtcc cggacggcga cctcgccgta
     2641 ggccgagagg tcgcgccgtg gatcgctcgt gtggagcgtg acgctgatgc gcttgtacgg
     2701 cgccatgcgc tcggcgaggt cggcgagggc gccgtcgtag agcagcacgc cgtggtggat
     2761 gacgatcacc cgccg
//
LOCUS       DASTZR010000173         2061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_924211, whole genome shotgun sequence.
ACCESSION   DASTZR010000173 DASTZR010000000
VERSION     DASTZR010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924211"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..1346
                     /gene="gatA"
                     /locus_tag="VFV20_03860"
     CDS             6..1346
                     /gene="gatA"
                     /locus_tag="VFV20_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEU5287514.1"
                     /translation="MFLATDRVRATESAKELTALIGRAEAPGNLAGVPVAVKDNIATL
                     HMPTTCGSKILEGYVSPYEATVVRRLRANGAVIIGKTNMDEFAMGSSTENSAYGPTRN
                     PVDIRRVPGGSSGGSAAAVAAGIVRLALGSETGGSVRQPASFCGVVGVKPTYGRVSRY
                     GLVAFGSSLDQVGVLGRSVQDTAIGLEVIAGCDQLDSTSADVPVPRYELEKNARLDGL
                     VIGKPKEYFPEGLDPRVAERCERALDAFRELGADVRDVSLPHTSYSIPVYYVVAPAEA
                     SSNLARFDGVRYGLRVASESGLTGMYEATRARGFGPEVTRRVMLGTYVLSAGYYDAYY
                     KKAQSVRTLIAQDFRDVFASGVDVLFTPTTPTPAFELGAVKDPYEMYLSDIFTASANL
                     AGVPAISVPIGTASGLPIGGQIIAGHFEESAMFRAAFGLERALAGKALPGEGSA"
     gene            1373..>2061
                     /gene="gatB"
                     /locus_tag="VFV20_03865"
     CDS             1373..>2061
                     /gene="gatB"
                     /locus_tag="VFV20_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEU5287515.1"
                     /translation="MVVGLEVHVQLKTRTKAFCACSADFGAPPNTNVCPVCLALPGAL
                     PVLNRHAVELALRAALALECKVNETSVFARKNYFYPDLPKGYQISQYDRPLAEKGWLT
                     IGELDEHTPVRVGITRVHMEEDAGKSVHDRYPGATAIDLNRAGVPLIEIVSEPDMRSA
                     AEAGAYLRLLKQILSYAGVSDVSMEEGSLRVDANVSARVRGTTKLGTKTEVKNMNSFS
                     AVERALDSEFAR"
BASE COUNT          340 a          680 c          721 g          320 t
ORIGIN      
        1 tcaacatctt tctggcgacc gatcgcgtcc gcgccaccga gagcgcgaag gagctgacgg
       61 cgctgatcgg tcgcgccgaa gccccgggca acctggcggg cgtgccggtg gcggtcaaag
      121 acaacatcgc cacgctgcac atgcccacga cgtgcggctc gaagatcctc gaaggctacg
      181 tgagcccgta cgaggccacg gtcgtgcgcc gcctccgggc caacggcgcc gttatcatcg
      241 gcaagacgaa catggatgag ttcgccatgg gatcgtccac cgagaacagc gcgtacggcc
      301 cgaccagaaa cccggtggac atccgccgcg tgccgggcgg atcctccggc ggttccgccg
      361 ccgccgtcgc cgctggaatc gtgcggttgg ccctcggctc ggagaccggc ggctccgtgc
      421 gccagccggc gtcgttctgc ggcgtcgtcg gcgtgaagcc tacgtatgga agggtgagca
      481 ggtacgggct cgtcgcgttc ggctcctcgc tcgaccaggt cggggtgctc ggccgctcgg
      541 tccaggacac cgcgatcgga ttggaagtca tcgcgggctg cgatcagctc gactccacca
      601 gtgccgatgt gcccgtgccg cgctacgagc tggagaagaa cgcgcggctc gacgggctcg
      661 tgatcggcaa gccgaaggag tatttcccgg agggactcga tccgcgcgtc gcggagcggt
      721 gcgagcgggc gctcgacgcc tttcgcgagc tgggcgcgga cgtgcgggac gtatcgctgc
      781 cgcacacgag ctactccatt ccggtgtatt acgtggtcgc gcccgcggag gcgtcgtcca
      841 atctcgcgcg gttcgacggc gtgcggtacg gcctgcgcgt cgcgtccgag agcgggctca
      901 ccggcatgta cgaagcaacg cgcgcgcgcg gcttcgggcc ggaggtcacg cgccgcgtga
      961 tgctcggcac gtacgtgctc tcggccggct attacgacgc ttactataag aaggcgcagt
     1021 cggtgcgaac gctgatcgcg caggattttc gcgacgtgtt cgcgagcggc gtggacgttc
     1081 tcttcacgcc gacgacgccg acgccggcgt tcgagctcgg agcggtgaag gacccgtacg
     1141 agatgtacct gagcgacata ttcaccgctt cggcgaatct cgcgggagtg cccgccatct
     1201 cggtcccgat cggaacggcg agcgggcttc cgatcggagg ccagatcatc gcgggtcact
     1261 tcgaggaatc cgcgatgttc cgcgccgcgt tcgggctcga gcgcgcgctg gcgggcaagg
     1321 ccctccccgg cgagggaagc gcgtgaacgc cgccgcgacc gccgattacg agatggtcgt
     1381 ggggctggaa gttcacgttc agctcaagac acgcaccaag gcgttctgcg cgtgctccgc
     1441 cgatttcggc gcgccgccga acaccaacgt ctgtcccgtc tgcctcgcgc tgcccggagc
     1501 cttgccggtg ctcaaccgcc acgccgtcga gctcgcgctg cgcgcggcgc tggcgctcga
     1561 gtgcaaggtc aacgagacgt ccgtgttcgc ccggaagaac tatttctatc cggacctccc
     1621 aaagggctat cagatctcgc agtacgaccg cccgctcgcg gagaagggct ggctcaccat
     1681 cggcgagctc gacgagcata cgccagtccg cgtcggaatc acgcgcgtgc acatggagga
     1741 ggacgcgggg aagtcggtgc acgaccgcta ccccggcgcg acggcaatcg acctgaaccg
     1801 cgcgggcgtg ccgctgatcg agatcgtgag cgaacccgac atgcgctcgg cggcggaagc
     1861 gggagcgtat ctgcggttat tgaagcagat cctgagctac gccggcgtca gcgacgtgag
     1921 catggaggag ggaagcctgc gcgtggacgc gaacgtcagc gcgcgcgttc gcggcacgac
     1981 gaagctcggc accaagaccg aggtgaagaa catgaactcc ttctccgcgg tcgagcgcgc
     2041 gctggactcc gagttcgcgc g
//
LOCUS       DASTZR010000174         3288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_973277, whole genome shotgun sequence.
ACCESSION   DASTZR010000174 DASTZR010000000
VERSION     DASTZR010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973277"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="VFV20_03870"
     CDS             complement(<1..794)
                     /locus_tag="VFV20_03870"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU5287516.1"
                     /translation="MTVQAADEIELEEAPAPTTRAAELARLSPARATLELAWPGIIEQ
                     SVSAAGTALIFAFVGHLGAVATAGAGAAGSFLFLLFPVWRSLAIGTIAIVSRRMGEGR
                     PAEAADATRQSLVLGALAGIAFGVGFIVFAAPLLRLLGANEDVVAVGAPFLAVIGASS
                     GASTVALIGTSAMRASGDTRTPMFLAAVTVVFGVVLAYVLIDVFKVGPIGAAWANAIT
                     WTLNVIAVLVILWRGVAGLSIAGGPWHLTMPTIRRIFGISLPSAGE"
     gene            complement(794..1837)
                     /gene="selD"
                     /locus_tag="VFV20_03875"
     CDS             complement(794..1837)
                     /gene="selD"
                     /locus_tag="VFV20_03875"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(1784..1786),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEU5287517.1"
                     /translation="MFTRDEVRLTTLSQCAGUGAKLGPGPLAQVLRPLSAQPVPPELI
                     VGLERADDAAVYLVAPDTAIVETLDFFPPIVDDAYASGAIAAANAMSDVYAMGGEVLF
                     ALQIAAWPEDAPLSQLERIFQGAVDTVRAAGGVVAGGHTVIDREPKFGLAVTGRVAPA
                     RILHKEGLRAGDVLWLTKPIGTGVLTTAHKRGDLSPEDLASTVASMVALNRDAARAAV
                     AANIHAGTDITGFGLAGHAHEMAERSGVAVRISMGAVPLLPGAHAHALGTSFGGLERN
                     QAFFLAEGRVRFEDVAEALRILALDPQTSGGLLLAVPAAHAKDWDDARRAHHVEAWRI
                     GEVTAGSGITITA"
     gene            complement(1860..2045)
                     /locus_tag="VFV20_03880"
     CDS             complement(1860..2045)
                     /locus_tag="VFV20_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287518.1"
                     /translation="MNALLIFLGLDLLLVAGAALLLLVVIVRRGAAPQYLALAALLIV
                     LAVGVWYTAIRTPSVLP"
     gene            complement(2042..3184)
                     /locus_tag="VFV20_03885"
     CDS             complement(2042..3184)
                     /locus_tag="VFV20_03885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5287519.1"
                     /translation="MERAEVVIVGGGVVGASIAWHLASRGVRDIVVIERDRLGSGSTS
                     RNAGGVRLQFSSEINVRLSQRSLPRWERFAEEMGVDPAFHQVGYLFLVTSDADAGAFE
                     RSLALWSRLDVPARRIDAQEARALFPELKVDDVRFATFCPKDGHLDPTALLNGYVARA
                     REKGVRFREGEPVTAIDRVGDRVSAVRTPQGTIACATVVNAAGPWAAQVGALAGVDLP
                     IQPLRRQIFVTDPVPGLDHEFPLTVEMATTFYFHRESGGVLMGMVDPADRPGFDDSLN
                     WDFLPTMVERALDRVPVLERANVKTGWAGFYEDTPDKHPIIGNVKALPGFVCAAGFSG
                     HGLMHAPAAGEAVAELITDGRTTLDISRLAYERFERGDLIREHNVI"
BASE COUNT          520 a         1170 c         1182 g          416 t
ORIGIN      
        1 ctctcacctg cggacggcag gctgatcccg aagatgcggc ggatggtcgg catcgtgagg
       61 tgccacgggc cgcccgcgat cgagagtccc gcgacgccgc gccagaggat gacgaggacc
      121 gcgatcacgt tgagcgtcca ggtgatggcg ttcgcccagg cggcgccaat cggaccgacc
      181 ttgaagacgt cgatgaggac gtacgccagc acgacgccga acacgaccgt gaccgccgcg
      241 aggaacatcg gcgtgcgcgt gtcgcctgat gcgcgcatcg cggacgtccc gatgagcgcc
      301 accgtggacg cgccgctgct cgcgccgatc accgcgagga acggcgcccc gacggcgacg
      361 acatcctcgt tcgcaccgag gagtcgcagg agcggagcgg cgaacacgat gaatccgacg
      421 ccgaacgcga tacccgcgag cgcgccgagc acgagcgact gccgcgtcgc gtccgcggct
      481 tcggcgggac gcccctcccc catccgccgg ctgacgatcg cgatcgtccc gatcgcgagc
      541 gatcgccaca ccgggaacag caggaacagg aagctgccgg ccgcgcccgc gcccgcggtc
      601 gccactgcgc cgaggtgccc gacgaaggcg aagatcagcg cggtgccggc ggcgctgacg
      661 gactgctcga tgatcccggg ccacgcgagc tcgagggtgg cgcgcgccgg cgaaagccgc
      721 gcgagctccg ccgcgcgcgt cgtcggtgcg ggtgcttcct cgagctcgat ctcgtcggcg
      781 gcctggacgg tcattacgcg gtgatcgtga tcccagaacc cgcggtgacc tcaccgatgc
      841 gccacgcctc gacgtggtgc gcgcgacggg cgtcgtccca gtccttcgcg tgcgcggccg
      901 gcaccgcgag cagcaggccg ccggacgtct gcgggtcgag cgcgaggatg cgcagcgcct
      961 ccgcgacgtc ctcgaaacga acgcgtccct ccgcgaggaa gaacgcctga ttgcgctcga
     1021 ggccgccgaa gctcgtgccg agcgcgtgcg cgtgcgcacc cggcagcagc ggcaccgcgc
     1081 ccatgctgat gcgcaccgcg acccctgagc gctctgccat ctcgtgcgcg tgaccggcga
     1141 ggccaaagcc ggtgatgtcc gtcccggcgt ggatgttcgc cgcgaccgcc gcacgcgccg
     1201 cgtcgcggtt cagggcgacc atcgacgcga cggtcgacgc gagatcctcg ggcgagagat
     1261 cgccgcgctt gtgtgcggtc gtgagcacgc cggtcccgat gggcttggtg agccagagca
     1321 catcacccgc gcggaggcct tccttgtgga ggatgcgcgc cggcgcgaca cgcccggtga
     1381 ccgcgaggcc gaacttgggc tcgcgatcga tcacggtgtg tccgcccgcg acgacgccgc
     1441 cggccgcgcg caccgtgtcg accgcgccct ggaagatccg ctcgagctgc gagagcggcg
     1501 cgtcctcggg ccacgccgcg atctgcagcg cgaagagcac ctcgccgccc atcgcgtaca
     1561 cgtccgacat cgcgttcgcg gcggcgatcg cgccggacgc gtacgcgtca tcgacgatcg
     1621 gcgggaagaa gtccagggtc tcgacgatcg ccgtgtcggg cgcgacgaga tacaccgccg
     1681 cgtcgtcggc gcgctcgagg ccgacgatca gctcgggcgg gaccggctgg gcactgaggg
     1741 ggcgcaggac ctgcgccagc gggccggggc ccagcttcgc ccctcagccg gcgcattggg
     1801 acagcgtggt gaggcggacc tcgtcccgtg tgaacatcag ctcagagtct agagatgcgt
     1861 cagggcagca ccgacggcgt ccggatcgcc gtgtaccaga cgccgacggc gaggacgatc
     1921 agcagggccg cgagcgcgag gtactgcggg gccgcgcccc tgcgaacgat gacgacgagg
     1981 agcagcagcg ccgcgccagc gacgagcagg aggtcgagcc cgaggaagat gagcagcgcg
     2041 ttcatatgac gttgtgctcg cggatgaggt cgccccgctc gaagcgctcg taagcgagcc
     2101 gagagatgtc gagcgtcgtc cgaccgtcgg tgatgagctc ggcgacggcc tcgccggcgg
     2161 cgggcgcgtg catgaggccg tggccgctga accccgcggc gcacacgaag ccgggcagcg
     2221 ctttcacgtt cccgatgatc ggatgtttgt cgggggtgtc ctcgtagaag ccggcccagc
     2281 ccgtcttcac gttcgcgcgc tccaggaccg gcacgcggtc gagcgcccgc tcgaccatcg
     2341 tcggcaggaa gtcccagttg agcgagtcgt cgaagcccgg ccggtccgcc ggatcgacca
     2401 tgcccatgag cacgccgccc gattcgcgat ggaagtagaa ggtggtcgcc atctcgaccg
     2461 tgagcgggaa ctcgtgatcg agcccgggca ccggatcggt gacgaagatc tggcgccgca
     2521 gcggctggat cgggagatcg acgcccgcga gcgcgccgac ctgtgcggcc cagggacccg
     2581 cggcgttcac gacggtcgcg cacgcgatcg tgccctgtgg cgtgcgcacc gccgacacgc
     2641 ggtcccctac gcggtcgatc gccgtcaccg gctcgccctc gcggaagcgc acgcccttct
     2701 ctctcgcgcg cgcgacatag ccgttgagca gtgccgtggg atcgagatgg ccgtccttcg
     2761 ggcagaaggt cgcgaagcgc acgtcgtcca ccttcagctc ggggaagaga gcgcgcgcct
     2821 cctgcgcgtc gatccgtcgc gcggggacgt cgagccgcga ccacagcgcg agcgaccgct
     2881 cgaacgcgcc ggcatccgcg tcgctggtga cgaggaagag atagcccacc tgatggaacg
     2941 cgggatcgac gcccatctcc tcggcgaatc gctcccagcg cggcagcgag cgctgcgaca
     3001 ggcgcacgtt gatctcgctc gagaactgca gccgcacgcc accggcattg cgtgacgtgg
     3061 agccgctgcc gagacggtcg cgctcgatga cgacgatgtc gcgcacgccc ctgctcgcga
     3121 ggtgccacgc gatggacgcg ccgaccaccc cgccgccgac gatgacgacc tcagcgcgct
     3181 ccaccctgcc cgatccgcct ccgctcactg atggagcggc gagtgccgct ccagagggcc
     3241 ggagccaagg gcgaaggtca cgactgctcg ggcggcacgg cggacggc
//
LOCUS       DASTZR010000175         3685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_297664, whole genome shotgun sequence.
ACCESSION   DASTZR010000175 DASTZR010000000
VERSION     DASTZR010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297664"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VFV20_03890"
     CDS             complement(<1..637)
                     /locus_tag="VFV20_03890"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5287520.1"
                     /translation="MSTLSVQAVGRWFGDRELLHDVSFRVADGDRVGLVGPNGTGKTT
                     LLRIAAGLEEPDAGNVAYARGTRLGFLRQELLEDATGTVDEHARGAAAHLRELERELH
                     ELEHALGGADQEALERYADLQHHFDHAGGYDFEATLRRVLAGLGLADLMDRQVVTLSG
                     GERTRLGLARLLLDDPDILLLDEPTNHLDVAALEWLEQFLVERDVTAVVSSH"
     gene            complement(642..1520)
                     /gene="mutM"
                     /locus_tag="VFV20_03895"
     CDS             complement(642..1520)
                     /gene="mutM"
                     /locus_tag="VFV20_03895"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEU5287521.1"
                     /translation="MPELPEVETIARQLRERVVGRRIADFVSYWHRVTDPMPAAHVAA
                     RLVGRRIAGVGRRGKFVVLELDNDEALIVSLRMTGRLLFQPADAPVDPYTRALIGFDD
                     GTVLRFADTRKFGRMAIVDLDELSADERTAARGAARAKQALHRALGIEPLSRRFTSSW
                     LGELLRRRSRAAIKPLLLDQRAIAGIGNIYAIEALWRARIHPLRGAGSLRPAEVVRLH
                     EAIRWVLRKGIRFGGASRRDYRDARGERGRMQQEFNVYDRAGEPCPRCGRAIARTVVG
                     GRGTFHCPRCQRAPAS"
     gene            complement(1541..>3685)
                     /gene="polA"
                     /locus_tag="VFV20_03900"
     CDS             complement(1541..>3685)
                     /gene="polA"
                     /locus_tag="VFV20_03900"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEU5287522.1"
                     /translation="PGVGEKTAAKLIAENGNIEGVYADLARFTPKLRENLAAHKEQVF
                     KSREMATIITDLPVTLEVERTKWRGYDRAAVLDLFRDLEFRSLIPRLPPADASLAGGP
                     IVTPAARPGQLALGLETAPATPPPVDTTLLDDAHAAEVAGIARDAGGLALHADVEVRR
                     HPDLVGLGLAVADRAWYLPASSGIPQALVDVLADERVPKTVHDAKTARRALRRAGAEL
                     RGVRMDTMLASYLVNASRRYHALEDLSAERLKLEVPVLPVADRKDPNRVATLEERVVR
                     AGAAAVAAARLGALFDEDLDRLEMRRLFETVELPLVDVLVEMEEAGIAVDVSYLRSLG
                     EEFAREVARIEREAYDAVGHDFPINSPKQLASLLFEELKLPRGRRTATGFSTDATVLE
                     ELRGVHPVVDKILEYREIEKLRSTYAEGLGALIDPATKRIHTTFDQAVAATGRLSSKN
                     PNLQNIPIRTPLGRRIRRAFVSGGPDLSLVAADYSQIELRVLAHVSGDPELTKAFREG
                     ADVHRRTAAAGFGVAEDTVTREQRDVAKMLNFGIVYGMSDFGLAWRMKMARDEAQRFI
                     DEYFKRYALVRRYVIETKAFCMERGYVETLLGRRRYIADMTSPNSAIRGAAERMAINM
                     PIQGTAADIMKIAMRRVYDRLAASKLRARVLLQVHDELVAEVPKGEVDEVASILCEEM
                     SNAYALDVPLVVDVRTGTNWDEMTRMQVPATA"
BASE COUNT          516 a         1300 c         1331 g          538 t
ORIGIN      
        1 cgtgactgct gacgaccgcg gtgacgtcac gctcgacgag gaactgctcg agccactcga
       61 gcgcggcgac gtcgagatgg ttcgtcggct cgtcgagcag caggatgtcc ggatcgtcga
      121 gcagcagtcg cgcgaggccg agccgcgtgc gctcgccgcc ggacagcgtc acgacctggc
      181 ggtccatgag gtccgcgagg ccgaggcccg cgagcacgcg acggagcgtc gcctcgaagt
      241 cgtaaccgcc ggcgtggtcg aaatggtgct gcaggtccgc atagcgctcg agcgcctcct
      301 gatccgcgcc gccgagcgcg tgctcgagct cgtgcagctc gcgttcgagc tcgcggaggt
      361 gcgccgccgc gccgcgcgcg tgctcatcga cggtgccggt cgcgtcttcg agcagctcct
      421 gccgcaggaa gccgagccgc gtcccgcgcg cgtaggcgac gttgcccgcg tcgggctcct
      481 cgaggccagc ggcgatgcgc agcagcgtgg tcttccccgt gccgttcggt ccgacgagac
      541 ccacacggtc gccgtcggcg acgcggaagg acacgtcgtg cagcagctcg cgatcgccga
      601 accaccggcc gacggcctgg acagagagtg tgctcacggg ctcatgatgc cggtgcccgc
      661 tgacaccgtg ggcagtggaa cgtgccgcgc ccgccgacga ccgtgcgcgc gatggcgcgg
      721 ccgcagcgcg gacagggctc cccggcacgg tcgtagacgt tgaactcctg ctgcatccgg
      781 cctcgctcgc cgcgggcgtc gcggtagtcg cgccgcgagg cgccgccaaa gcggatgcct
      841 ttccgcagca cccagcggat cgcctcgtgc aggcgcacga cctcggcagg ccggagcgag
      901 ccggcgccgc gcagcggatg gatgcgtgcc cgccagagcg cctcgatcgc gtagatgttg
      961 ccgatcccgg cgatggcgcg ctggtcgagc aggagcggct tgatggcggc ccgggagcgg
     1021 cggcgcagca gctcaccgag ccacgacgac gtgaagcgcc gcgacagcgg ctcgatcccg
     1081 agcgcgcggt ggagcgcctg cttcgcgcgg gccgcaccgc gtgcggccgt ccgttcgtcg
     1141 gcgctcagct cgtcgaggtc gacgatcgcc atgcgcccga acttccgcgt gtccgcgaag
     1201 cgcaggaccg tgccgtcgtc gaagccaatg agcgcccgcg tatatggatc caccggcgcg
     1261 tccgcgggct ggaacaggag ccggccggtc atgcggagcg acacgatgag cgcctcgtcg
     1321 ttgtccagct cgagcacgac gaacttcccg cgccgtccca cgcccgcgat gcggcggccg
     1381 acaaggcgcg cggcgacgtg cgcggcgggc atcggatcgg tgacccgatg ccaatacgag
     1441 acgaagtcgg cgatgcgccg tcccacgacg cgttcgcgca gctgccgtgc gatggtctcg
     1501 acctcgggga gctcaggcat ggcggaggga cacgggccga tcaggcagtg gccggcacct
     1561 gcattcgtgt catctcgtcc cagtttgtgc cggtgcgcac gtcgacgacg aggggcacgt
     1621 cgagcgcgta ggcgttcgac atctcctcgc acaggatcga agcgacctcg tcgacctcgc
     1681 ccttcgggac ctcggccacg agctcgtcgt ggacctggag gagcacgcgc gcgcgcagct
     1741 tcgacgccgc gagccggtcg tacacgcgcc gcatcgcgat cttcatgatg tcggcggcgg
     1801 tcccctggat gggcatgttg atcgccatgc gctcggcggc tccgcggatg gcgctgttcg
     1861 ggctcgtcat gtcggcgatg taccgtcggc gtccgagcag cgtctccacg tagccgcgct
     1921 ccatgcagaa tgccttcgtc tcgatgacgt agcggcgcac gagcgcgtac cgcttgaagt
     1981 actcgtcgat gaagcgctgc gcctcgtcgc gcgccatctt catgcgccac gcgaggccga
     2041 agtcgctcat gccgtagacg atgccgaaat tcagcatctt cgcgacgtcg cgctgctcgc
     2101 gcgtcacggt gtcctcggcg acgccgaatc ccgcggcggc cgtgcgccgg tgcacgtcgg
     2161 caccctcgcg gaacgccttg gtgagctcgg ggtcgccgga cacgtgcgcg agcacccgga
     2221 gctcgatctg cgagtagtcc gccgcgacga ggctgaggtc cgggccgccg gagacgaacg
     2281 cgcggcggat gcggcggccg aggggcgtgc ggatggggat gttctgcaga ttcggattct
     2341 tgctggagag ccgtccggtc gcggcgacgg cctggtcgaa ggtcgtgtgg atgcgcttcg
     2401 tggccgggtc gatgagcgcg ccaagcccct ccgcgtacgt cgaacgcagc ttctcgatct
     2461 cgcggtactc gaggatcttg tccaccaccg gatgcacgcc gcgcagctcc tcgagcacgg
     2521 tcgcgtcggt ggagaagccg gtcgccgtcc gccggccacg cggcagcttg agctcctcga
     2581 acaggaggga cgcgagctgc ttcggcgagt tgatcgggaa gtcgtggccg acggcgtcgt
     2641 aagcctcgcg ctcgatgcgc gcgacctcgc gtgcgaattc ctcgccgagc gagcgcaggt
     2701 agctcacgtc caccgcaatg ccggcctcct ccatctccac gagcacgtcc acgagcggca
     2761 gctccacggt ctcgaagagc cgccgcatct cgaggcggtc gagatcctcg tcgaagagcg
     2821 cgccgagccg ggcggccgcg acggcggcgg cgcccgcgcg cacgacgcgc tcctcgagcg
     2881 tcgcgacacg gttcggatcc ttgcgatccg cgacgggcag caccggcacc tcgagcttga
     2941 gccgctccgc ggagaggtcc tcgagcgcgt ggtagcgacg gctggcgttg acgaggtacg
     3001 acgcgagcat cgtgtccatg cgtacgccgc gcagctcggc accggcacgc cgcagcgcac
     3061 gtcgggccgt cttcgcgtcg tggacggtct tcggcacgcg ctcgtccgcc aggacgtcca
     3121 ccagcgcctg cgggatgccg ctcgacgcgg ggagatacca cgcgcgatcg gcgacggcga
     3181 gcccgagtcc cacgagatcc gggtggcgac gtacctcgac gtccgcgtgg agcgcgagcc
     3241 cgcccgcgtc gcgcgcgatc cccgcgacct cggccgcatg cgcgtcgtcg agcagcgtcg
     3301 tgtccaccgg cggcggcgtc gccggcgcgg tctcgaggcc gagcgcgagc tggcccggac
     3361 gcgctgcggg cgtcacgatc gggccgcccg ccaggctggc gtcggccggt ggcagccggg
     3421 ggatgaggct gcggaactcg aggtcacgga agagatcgag caccgccgcg cggtcgtagc
     3481 cgcgccactt cgtgcgctcg acctcgagcg tcaccggcag atccgtgatg atcgtcgcca
     3541 tctcgcggct cttgaagacc tgctccttgt gcgccgcgag gttctcgcgc agcttcggcg
     3601 tgaaccgcgc gaggtcggcg tagacgccct cgatgttgcc gttctcggcg atgagcttcg
     3661 ccgcggtctt ctcccccacg cccgg
//
LOCUS       DASTZR010000176         4255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_114853, whole genome shotgun sequence.
ACCESSION   DASTZR010000176 DASTZR010000000
VERSION     DASTZR010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114853"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="VFV20_03905"
     CDS             <1..564
                     /locus_tag="VFV20_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287523.1"
                     /translation="DTSIQPGSSTGKRCRASAGTVQVCNASYGRNGWLGLASIWLSGS
                     HIVQGTVKVNDTYFVMAAYNNSSEKLHVMCQEVGHTFGLGHQDETGISLNTCMDYYHN
                     TSDADTKSTHPNQHDYDELVTIYTNHFDAAAGASAGSAGAAVGVRGVDADGTPIGASR
                     ARGSWYVQDLGAGNFLLTHVFWSPGGH"
     gene            662..1687
                     /locus_tag="VFV20_03910"
     CDS             662..1687
                     /locus_tag="VFV20_03910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HEU5287524.1"
                     /translation="MIEPIRVVCVGGGLGAPTVMRGLRAHTQDITGLIGVTDSGRSTG
                     KVRIALDVPAPGDIRNALSVLAEGDPVLTRLMAHRFETDKSEDLNGMAFGNLLLAALT
                     QQEGSFLRAVEEASRLLKLRGRVLPVTLYNTHLCAELADGTIVEEEVNVRAVGKPPIR
                     RIFLKAGDEVTATPGSVEAIAAADLITLGPGSLYTTVCACLLVPEIARAIANARGLVV
                     YVANTTRQPGQTDEMGLADHVRVVRDYLGGSGLDVALVNDDAPPEHLRRHYADQGLEY
                     MEPTALELERIRALGVRPVTGPIIDKWSGPRDLWLKQDTIRHDAERVARALVSLLDER
                     RPALRAL"
     gene            1687..2607
                     /gene="argF"
                     /locus_tag="VFV20_03915"
     CDS             1687..2607
                     /gene="argF"
                     /locus_tag="VFV20_03915"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010878750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU5287525.1"
                     /translation="MRHLTSILDIDARELTELLGLAGELRTDRAARTMQLRGRTLAQI
                     FEKPSLRTRLSFDVGMSELGGHCVYLSPQEVGLGRRESVADVARVVSRMVDAVVLRTN
                     SHETIEEFARCSTIPVINGLSDLSHPCQALADILTISEKKGTDLHGVTIAYVGDGNNV
                     LHSLMLVAALRGARMRVATPEGYEPHQRYIEASQRLAAEQRGSLDLLHDPVAAVTGAD
                     VVYTDVWTSMGQEQEYERRRRAFLGYQVNAQLLRHAKPDAIVMHDLPAHRGEEITDDV
                     MDGPQSVVFDQAENRLHAQKAVLWWLLKDA"
     gene            2607..3086
                     /locus_tag="VFV20_03920"
     CDS             2607..3086
                     /locus_tag="VFV20_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287526.1"
                     /translation="MNDRRRGDERVILADCCEDWIIEWGGYYKAERAFRCPECGTEWT
                     KSAADTYRRADGRTFQRRTRVGPQASFPYLASVDGHQPNVERCCAKILLAHGERMPEG
                     AFVCPVCGTAWQKSTERLHGLRIAIFAKPGLAEPLTIQPGRTRPFLVALSEYSPPRE"
     gene            3274..>4255
                     /locus_tag="VFV20_03925"
     CDS             3274..>4255
                     /locus_tag="VFV20_03925"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEU5287527.1"
                     /translation="MRASRLRFLAAALALGFVLALVAGAAGLLGGSRAFAGGTTLTVI
                     GGEVAVRHGASDFVAAADGETLNPGDVIRTGVDGRAVLTYFEGSTVTVEPGTELAIDS
                     AAVAPDGGTVVLMTQTAGRTWHVVTKLITGGSKYEVRTPASTASVRGTEFQVDASDDA
                     TTVTTTEGTVVQHVPDPARPNTTVDVPVTAGTTQTQQRNAPPAPTKTAPEPSRKVTVT
                     VGAANTLVIDPLGRANGLTKDGKLVVQTPGAQVRREDGRIIVTLPELPDGKVTARTDK
                     KDENDDGDVAVTARVEEQGKVLDLEDRTKVEGSKKVAGFEVRRRSEVRRRS"
BASE COUNT          700 a         1479 c         1482 g          594 t
ORIGIN      
        1 gacacgtcca tccagcccgg ttccagcacc ggcaagcgat gccgggccag cgccggaacg
       61 gtccaggtgt gcaacgcctc gtacggccgc aacggctggc tcggcctggc gtcgatctgg
      121 ctctccggtt cgcacatcgt gcagggcacc gtgaaggtca acgacaccta cttcgtgatg
      181 gccgcgtaca acaacagcag cgagaagctc cacgtcatgt gccaggaggt cggacacacc
      241 ttcggcctgg ggcatcagga cgagaccggg atctcgctga acacctgcat ggactactac
      301 cacaacacga gtgacgcgga cacgaagagc acgcatccga accagcacga ttacgacgag
      361 ctcgtcacca tctacacgaa ccacttcgac gcggcagccg gggccagcgc gggtagcgcc
      421 ggcgcggccg tcggtgttcg gggcgtcgac gcggatggca cgccgatcgg cgcgtcacgc
      481 gctcgcggca gctggtacgt ccaggatctc ggagcgggta acttcctgct cacgcacgtc
      541 ttctggtcgc cgggcggcca ctaagagccg atctcgtcac gccgaaggac acggagggcc
      601 ccgggatacc ggggccctcc ttctttctgg tcctcttctg catatctgcc aagattccag
      661 cgtgatcgag cccatccgtg tcgtgtgcgt cggcggcggc ctcggggcgc cgacggtgat
      721 gcgtgggctt cgcgcgcaca cccaggacat caccgggctc atcggcgtca ccgactcggg
      781 gcgctcgacg gggaaagtgc ggatcgcgct cgacgtgccc gcacccggcg acatccgcaa
      841 tgcgctgagc gtgctcgcgg agggcgatcc ggtgctcacg cggctcatgg cgcatcgctt
      901 cgagaccgac aagagcgagg acctgaacgg catggccttc ggcaacctgc tcctcgccgc
      961 gctcacgcag caggagggct ccttcctgcg cgcggtcgag gaggcgtccc gcctgctgaa
     1021 gctgcgtggt cgcgtgctgc cggtcaccct gtacaacacg cacctctgcg ccgagctcgc
     1081 cgacggcacg atcgtcgagg aagaggtcaa cgtgcgcgcg gtcggcaagc cgccgatccg
     1141 gcgcatcttc ctcaaggcag gggacgaggt gaccgcgaca ccggggagcg tcgaggcgat
     1201 cgccgccgcc gatctcatca cgctcggacc gggcagcctg tacacgaccg tgtgcgcgtg
     1261 ccttctcgtg cccgagatcg cgcgcgcgat cgcgaacgcg cgcgggctgg tggtgtacgt
     1321 cgcgaacacg acacgccagc cggggcagac ggacgagatg gggctggcgg atcacgtgcg
     1381 cgtcgtgcgt gactacctcg gcgggagcgg tctcgacgtc gccctcgtca acgacgacgc
     1441 gccgccggag cacctgcgcc ggcactacgc cgatcagggt ctggagtaca tggagccgac
     1501 cgcgctcgag ctcgagcgga tccgcgcgct cggggtgcgt cccgtcaccg gaccgatcat
     1561 cgacaagtgg agcggtccgc gcgacctctg gctcaagcag gacaccatcc gccacgacgc
     1621 cgagcgggtc gcgcgcgctc ttgtgtcgct cctcgacgag cggcggccag cgctgcgcgc
     1681 tctgtgatgc gccatctcac ctccatcctc gacatcgacg cgcgcgagct cacagagctg
     1741 ctgggcctcg ccggcgagct ccgcacggac cgcgccgcgc gcacgatgca gctgcgcggg
     1801 cggacgctcg cgcagatctt cgagaagccc agcctgcgca cgcgcctgtc gttcgacgtc
     1861 ggcatgtccg agctcggcgg ccactgcgtg tatctgtcgc cgcaggaggt tgggctcggc
     1921 aggcgcgaga gcgtcgccga cgtcgcgcgc gtcgtgagcc ggatggtgga cgccgtcgtg
     1981 ctgcgcacga actcgcacga gacgatcgag gagttcgccc gctgctcgac gatccccgtc
     2041 atcaacggcc tctccgatct ctcgcacccg tgccaggcgc tcgcggacat cctcacgatc
     2101 agcgagaaga aggggaccga tctgcacggc gtgaccatcg cctacgtcgg cgacgggaac
     2161 aacgtgctgc attccctcat gctcgtcgcc gcgctgcgcg gcgcgcgcat gcgcgtggcg
     2221 acgccggagg gctacgagcc gcaccagcgg tacatcgagg cgtcccagcg tctcgcggcg
     2281 gagcagcgcg gatcgctcga cctcctgcac gatccggtcg cggccgtgac cggagccgat
     2341 gtggtgtaca ccgacgtatg gacgagcatg gggcaggagc aggagtacga gcgccgccgc
     2401 cgcgcattcc tcggatacca ggtgaacgcg cagctgctgc ggcacgcgaa gcccgacgcg
     2461 atcgtgatgc acgacctgcc cgcccatcgg ggcgaggaga tcaccgacga cgtgatggac
     2521 ggaccgcaga gcgtcgtctt cgatcaggcc gagaaccgcc tgcacgcgca gaaggccgtg
     2581 ctctggtggc tgttgaagga tgcgtgatga acgaccgacg acgcggtgac gagcgcgtga
     2641 tcctggccga ttgctgcgag gactggatca tcgaatgggg cgggtactac aaggccgagc
     2701 gcgccttccg ctgtcccgag tgcgggaccg agtggacgaa gagcgcggcc gacacgtacc
     2761 gccgcgccga cggccgcacc ttccagcggc gcacgcgcgt cgggccgcag gcgtcctttc
     2821 cgtacctggc ctccgtggac gggcatcagc cgaacgtgga gcgctgctgc gcgaagatcc
     2881 tcctcgcgca cggcgagcgc atgccggagg gcgcgttcgt gtgccccgtg tgcgggaccg
     2941 cgtggcagaa gagcaccgaa cggctgcacg ggctccgcat cgcgatcttc gcgaagcccg
     3001 gactcgccga gccgctcacg atccagccgg gtcgcacgcg gcccttcctc gtagctcttt
     3061 cggagtactc gccaccacgc gagtgacgga tgcgcgctcg tcatcgtgaa tggatgacac
     3121 ggcgctgaga cacgatgtcg aaggctgaga cgtctctgag accggcggcc ccccgaacga
     3181 ccccttacca agcgcccatg cgtcgttagg gtcatcgaac ggccccagcg ggcgggcggc
     3241 gcccaaaagg ggtcgcggtg tggggaggct ccagtgcgag cgtcgcgtct tcgctttctc
     3301 gcggccgcgc ttgctctcgg cttcgtgctc gcgctcgtgg ccggcgcggc gggtctgctg
     3361 ggcggatcgc gcgcattcgc cggcggtacg acgctcaccg tcatcggcgg tgaggtcgcg
     3421 gtgcgccacg gcgcatccga cttcgtggcc gccgccgacg gggagacgct gaaccccggc
     3481 gacgtcatcc gcaccggcgt cgacggccgt gccgtcctca cgtacttcga aggctccacc
     3541 gtgaccgtcg agccgggcac cgagctcgcg atcgacagcg cggcggtcgc gccggatggc
     3601 ggcaccgtcg tgctcatgac gcagaccgcc gggcgcacgt ggcacgtcgt gacgaagctc
     3661 atcaccggcg gctccaagta cgaggtccgc acgccggcca gcaccgcgtc cgtgcgtggc
     3721 accgagttcc aggtcgacgc atcggacgac gcgaccaccg tgacgacgac agagggcacc
     3781 gtcgtgcagc acgtcccaga cccggcgcgg ccgaacacga ccgtcgacgt gccggtgacc
     3841 gcggggacga cgcagacgca gcagcggaac gcgccgcccg cgccgacgaa gaccgcgccc
     3901 gagccgagcc gcaaggtgac ggtgacggtc ggtgccgcga acacgctcgt gatcgacccg
     3961 ctcgggcggg ccaacggcct gaccaaggac gggaagctcg tggtgcagac gcccggtgcg
     4021 caggtgcgcc gcgaagacgg ccggatcatc gtcacgctcc ccgagctgcc ggatggcaag
     4081 gtcaccgcgc gcaccgacaa aaaggacgag aacgacgacg gtgacgtcgc ggtcaccgcg
     4141 cgcgtcgagg agcagggcaa ggtcctcgac ctcgaggacc gcacgaaggt cgaaggatcg
     4201 aagaaggtcg ccggcttcga ggtgcgtcgc cgttcagagg tgcgtcgccg ttcag
//
LOCUS       DASTZR010000177         4668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1107107, whole genome shotgun sequence.
ACCESSION   DASTZR010000177 DASTZR010000000
VERSION     DASTZR010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107107"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VFV20_03930"
     CDS             complement(<1..677)
                     /locus_tag="VFV20_03930"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEU5287528.1"
                     /translation="MTADRKRWTENLQGEVDGAHVYRAMAKSESDASLATVYGRLAET
                     EERHGDLWRAKLREAGDGHVAVTPSWRARLLAFVAARAGAGMVASTLASREARDQRNY
                     DDQPEAGVALPRDERSHVRVLKEISGGGMAGPAIARLEGRHRATGGNALRAAVLGIDD
                     GLVSNFGLVMGVAGAGANSQALVVAGLAGLLAGALSMALGEWLSVQSARELFARQIAI
                     EREELETA"
     gene            829..2139
                     /locus_tag="VFV20_03935"
     CDS             829..2139
                     /locus_tag="VFV20_03935"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HEU5287529.1"
                     /translation="MRGVLSALLGTAALVMAACGTSARPEPTPTASAVPTASATVAPT
                     PLPTATPAASRIDHDVVYFARDRLPPVAAHVDGAGTGATAEARILSRMTALFGASAKP
                     PLFNTAPAAKARPASVRVAGDLATVDFTVPLGDWGTAGSTGTRAFIQQLVYTASEEPG
                     IRRVLITENGQQAIIGGEGVVIDHASTREDVAGYAVRASKEPMTFRTEPRRQPIPIVS
                     RLGVDTVAPSLVRFVVDTGLSGADAKAAIGFTASVVQNDETAFPDLGKWVIALALPDT
                     RTSEDAVRSFERTPVRSVRATTSSEGVRYEIGLDDLRPWRIAMQYEPLRIVLDIGGDP
                     DAVSPNIALYTPAFGTTVAAGDPISGFIRAFEARFEYRISDARGPVADEFASASLGTS
                     ELWGGFSVSIPAGLRGAATIEILLRSPKDGSVSESVFTSVQTSP"
     gene            complement(2158..2667)
                     /locus_tag="VFV20_03940"
     CDS             complement(2158..2667)
                     /locus_tag="VFV20_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287530.1"
                     /translation="MDTRRHWLLVTSEIGARDIARAQWRAYPLASATLPRPAAGDRVV
                     VLRTHRRSYVRSAVLIGTADVRGDTGNELELRHRFVAPAGHDVKLAAVPHLLVSHGWT
                     DERLGALMGLLVPLGPADYAAIEAALRIVALTYGPEASRPAHRLPRTPGRRRLITARL
                     MNRKPSPAR"
     gene            complement(2763..3413)
                     /locus_tag="VFV20_03945"
     CDS             complement(2763..3413)
                     /locus_tag="VFV20_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014047090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5287531.1"
                     /translation="MTEGQVRVMLVDDHEVVREGLRTLVGKNKGLIVVAEASTMAEAI
                     ETAGRAKPDVVIMDVRLPDGSGVEACRTIREARPETKVIMLTSYADDEALFASIVAGA
                     AGYLLKQTRGQAVIDAITSVAQGRSLLDPDVTGKVLERVRRGRDEDDPAFASLTDQER
                     KVLEQLAEGKTNREIGEVLFLSEKTVKNYVSRILDKLGLSRRAEAAAYMAKRRPRF"
     gene            complement(3523..>4668)
                     /locus_tag="VFV20_03950"
     CDS             complement(3523..>4668)
                     /locus_tag="VFV20_03950"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEU5287532.1"
                     /translation="DALEEKRTQLLAVNAAGLALTSELDRDAVLQRVADQARLVTNAK
                     YAALGVFDDEGVVETFTTSGISPAERERIGHLPRGRGLLGHLQKVQTPVRLRDLHEHP
                     SSVGFPANHPPMTSFLGTPILLRGEPLGNLYLTEKQGAAEFTEDDTEALKTLAAQAAI
                     AIENARLYEQAERVSVLEERNRIRMDLHDGVMQALYGVSLLLEDVADRVAEEPEHAKS
                     QLARAVDRLNAAIADLRGYVLGLRPIQATEQPLSESLALLAEQARSNALIDVDVDIAS
                     DAATKLDRARREAAYYIAADALGNIARHARARHATVRLFANDGSVILQITDDGVGFDF
                     RTSTTGHGLHNMRERAFAVGGTLEVHSAPGSGARVRLELPAKQEATE"
BASE COUNT          599 a         1696 c         1672 g          701 t
ORIGIN      
        1 gcggtctcga gctcctcgcg ctcgatcgcg atctgccgcg cgaagagctc gcgggcgctc
       61 tggaccgaga gccattcgcc gagcgccatg gagagcgctc ccgccagcag gccggcgagc
      121 cccgcgacga cgagcgcctg cgagttggcg cccgctccgg cgacgcccat gaccaggccg
      181 aagttggaca cgaggccgtc gtcgatgccg aggacggccg cgcggagcgc gttgccgccg
      241 gtggcgcggt ggcgtccctc gaggcgcgcg atcgccgggc cggccatgcc gccgcccgag
      301 atctccttca gcacgcggac gtggctgcgc tcgtcgcggg gaagcgcgac gcccgcctcc
      361 ggctggtcgt cgtagttgcg ctggtcgcgc gcctcgcgcg aggcgagggt cgaggcgacc
      421 atccccgcac cggcccgtgc cgcgacgaac gcgaggaggc gcgcgcgcca cgacggcgtg
      481 accgcgacat gtccgtcccc ggcctcgcgc agcttcgcgc gccagaggtc gccgtgccgt
      541 tcctcggtct cggcaaggcg gccgtacacg gtggcgagcg acgcgtcgct ctcgctcttc
      601 gccatcgcgc ggtagacgtg tgcgccgtcc acctcgccct ggaggttctc ggtccagcgc
      661 ttcctgtcgg cggtcatgtc agtcgatggg cgcacgtgtt tcgccaccgt cctgctctga
      721 ccctacggtc gcggcatccg cacccctatg ggcgagcggc ccgagtgtga ggggtcaaag
      781 gtccttccgg ccctcgctcc gcacgccgtc cggtgcgacg atcggcgcat gagaggcgtc
      841 ctcagcgcgc tgctcggcac ggccgcgctc gtcatggccg cgtgcggtac gagcgcccgg
      901 ccggagccca cgccgacggc gagcgccgtg ccgaccgcgt cggcgaccgt cgcgccgacg
      961 ccgctcccca ccgccacgcc cgcggcgagc cggatcgacc acgatgtcgt gtacttcgcc
     1021 cgcgaccgcc tgccgccggt cgcggcgcac gtcgacggtg ccggcaccgg cgcgacggcc
     1081 gaggcgcgca tcctctcgcg gatgacggcg ctcttcggcg cgagtgcgaa gccgccgctc
     1141 ttcaacaccg cgcccgcggc gaaggcgcgt ccggcgtcgg tccgcgtggc cggcgacctc
     1201 gcgacagtgg acttcacggt gccgctcggc gactggggca ccgcgggcag caccggtacg
     1261 cgcgcgttca tccagcagct cgtgtacacg gcgtcggagg agccgggcat ccgccgcgtg
     1321 ctcatcaccg agaacggaca gcaggcgatc atcggcggcg agggcgtcgt catcgaccac
     1381 gcctcgaccc gtgaggacgt cgcggggtac gcggtccgtg cctccaagga gccgatgacc
     1441 ttccgcaccg agccgcggag acagccgatc cccatcgtct cccgtctcgg cgtcgacacc
     1501 gtcgcgccct ccctcgtgcg gttcgtcgtc gacaccggcc tttccggcgc ggacgcgaag
     1561 gccgcgatcg gcttcaccgc gagcgtcgtg cagaacgacg agaccgcgtt ccccgacctc
     1621 ggcaagtggg tgatcgcgct cgcgctgccg gacacgcgca cgtccgagga tgcggtgcgc
     1681 tcgttcgagc ggacgcccgt gcgatcggtc cgcgcgacga cgagcagcga gggggtgcgc
     1741 tacgagatcg ggctcgacga tctgcggcca tggcgcatcg cgatgcagta cgagccgctg
     1801 cggatcgtgc tcgacatcgg cggcgatccc gacgcggtga gcccgaacat cgcgctgtac
     1861 acgcccgcgt tcgggacgac cgtggcggcg ggcgacccca tctccggctt catccgagcc
     1921 ttcgaggcgc gcttcgagta ccgcatcagc gacgcgcgcg gcccggtcgc cgacgagttc
     1981 gcgtcggcgt cgctcggcac gagcgagctc tggggcggct tcagcgtatc gatcccggcc
     2041 ggtctgcgtg gagcggccac catcgagatc ctgctgcgca gcccaaagga cggatcagtc
     2101 agcgagtcgg tgttcacgtc cgtgcagacg tcgccctgag gcggcgtgcg ccgaggctca
     2161 gcgggcgggg gacggcttcc gattcatgag gcgcgccgtg atgagacggc ggcgtccggg
     2221 cgtgcgcggg aggcggtgcg ccggccgtga cgcctcgggt ccgtacgtga gcgcgacgat
     2281 ccgcagcgcc gcctcgatgg ccgcgtagtc ggcggggccg agcgggacga gcagacccat
     2341 gagcgcgccg agacgctcgt cggtccagcc gtgcgagacc aggaggtgcg ggacggcggc
     2401 gagcttcacg tcgtggccgg cgggtgcgac gaaccggtgc cgcagctcga gctcgttgcc
     2461 ggtgtcacca cgcacgtcgg cggtcccgat gagcaccgcc gatctcacgt atgaacgccg
     2521 atgggtccgc agcacgacga cacgatcgcc tgccgcgggc cgtggcaggg tcgcgctcgc
     2581 gagggggtag gcgcgccact gcgcgcgcgc gatgtcccgc gcgccgatct cgctggtgac
     2641 gaggagccag tgtcgccggg tatccatgcg tcgaacgcta cggccggcgc atggcatgcg
     2701 tcatcgggcg acggtcccga agcgcgcgct gtcctacccg accgcggccg ggccgttcgg
     2761 cgctagaagc ggggccgccg cttcgccatg tacgccgccg cctccgcgcg gcgcgacagg
     2821 cccagcttgt cgaggatgcg gctcacgtag ttcttgacgg tcttctccga gaggaacagg
     2881 acctcgccga tctcccggtt cgtctttccc tccgcgagct gctcgaggac cttgcgctcc
     2941 tggtcggtga gcgaggcgaa ggcggggtca tcctcgtcgc ggccacgccg gacgcgttcg
     3001 aggaccttgc cggtgacgtc cgggtccagc agcgagcggc cctgcgcgac cgacgtgatc
     3061 gcgtcgatca ccgcctgccc gcgtgtctgc ttcagcaggt agccagccgc gcccgcgacg
     3121 atcgacgcga acagggcctc gtcgtcggcg tacgaggtca gcatgatcac cttggtctcc
     3181 ggccgagcct cgcggatcgt gcggcacgct tcgacgccgc tgccgtccgg caggcggacg
     3241 tccatgatca cgacgtccgg cttggcccgt ccggccgtct cgatcgcctc ggccatggtg
     3301 gaggcttcgg cgacgacgat gagccccttg ttcttcccga cgagcgtgcg caggccttcg
     3361 cggaccacct cgtggtcgtc gacgagcatc acgcggactt gaccttcggt catcttgtcg
     3421 cgctcggctc ggcccgcttc gcggcctgca cctcgccgcc tcgcccttcg ggctcgctgg
     3481 cagtgctcgc tcgtcgcggg tgaaccgctc ctcgctcgcg cttcattctg tcgcctcctg
     3541 cttcgccggt agctccaagc gtactcgcgc gccggaaccg ggcgcactgt ggacctcgag
     3601 cgtgccgccg acggcgaatg cgcgttcgcg catgttgtgg agaccgtggc cggtggtgct
     3661 cgtgcggaag tcgaagccca cgccgtcgtc ggtgatctgc aggatgaccg agccgtcgtt
     3721 cgcgaagagc cgcaccgtcg cgtgccgcgc ccgcgcgtga cgggcgatgt tgcccagcgc
     3781 gtccgcggcg atgtagtacg cggcctcgcg ccgcgcgcgg tccagcttgg tcgcggcgtc
     3841 ggaggcgatg tcgacgtcca cgtcgatgag cgcgttcgag cgggcctgct ccgcgagcag
     3901 cgcgagcgac tccgagagcg gctgctcggt ggcctggatg ggtcggagac cgagcacgta
     3961 cccgcgaagg tccgcgatcg ccgcgttcag gcggtccacc gcccgcgcga gctgcgactt
     4021 cgcgtgctcc ggctcctcgg ccacgcgatc ggcgacgtcc tcgagcagca ggctcacacc
     4081 gtagagcgcc tgcatcacgc cgtcgtgcag gtccatgcgg atccggttgc gctcctccag
     4141 gaccgaaacg cgctcggcct gctcgtagag ccgcgcgttc tcgatcgcga tcgcggcctg
     4201 cgcggcgagc gtcttgaggg cctccgtgtc gtcctcggtg aactccgctg cgccctgctt
     4261 ctccgtgagg tagagattcc cgagcggctc gccgcggagc aggatcggcg tcccgagaaa
     4321 gctcgtcatc ggcggatggt tcgcggggaa cccgaccgac gacggatgct cgtgcagatc
     4381 ccggagccgg acgggcgtct gcaccttctg cagatggccg agcagcccac ggccacgcgg
     4441 gagatgaccg atgcgctcgc gctccgccgg actgatgccg gacgtcgtga acgtctccac
     4501 gacgccctcg tcgtcgaaca cgccgagcgc ggcgtacttg gcgttcgtca cgaggcgcgc
     4561 ctggtcggcg acccgctgca gcaccgcgtc ccggtcgagc tccgatgtga gcgcgaggcc
     4621 ggcggcgttc actgccagca gctgcgtgcg cttctcctcg agggcgtc
//
LOCUS       DASTZR010000178         2920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_741713, whole genome shotgun sequence.
ACCESSION   DASTZR010000178 DASTZR010000000
VERSION     DASTZR010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741713"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VFV20_03955"
     CDS             complement(<1..464)
                     /locus_tag="VFV20_03955"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU5287533.1"
                     /translation="MPEERRVVSVLFADVAGSTALGESTDPEDVRALLGRYYAIAKDV
                     VAAHGGTLEKFIGDAVMAVFGIPQAHGDDAERALAAALALRERVAADPATAGLVLRIG
                     VNTGEVVATREAGAGDFLVTGDAVNVAARLQQSAEPGAIVAGERTRRATRAFR"
     gene            529..2340
                     /gene="lepA"
                     /locus_tag="VFV20_03960"
     CDS             529..2340
                     /gene="lepA"
                     /locus_tag="VFV20_03960"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEU5287534.1"
                     /translation="MADPQTRIRNFCVIAHVDHGKTTLSDRLIELTKTVPLRQMRAQM
                     LDSMELEREHGVTIKARAVRMAWPGQDGEIYELNLIDTPGHVDFNYEVSRSLAACEGA
                     LLLVDASQGIEAQTLANAYLAVEQGLEIIPVINKVDLPAIDLDVVREDLRTTLGFKDE
                     EILLASGKTGLGVEDLLNAIVDRVPPPSGDAAAPLRALIFDSHYDTYKGVVAHVRVVD
                     GSLAAEDRILGMATQKSSELLELGVFAPDAVPVQRLEAGEVGYVATGLKTVADCRVGD
                     TLTLEARPAAEPLPGYRPAKPMVFAGFYPQRAEDYPELRESLEKLRLNDASLVYEPET
                     SQALGFGFRCGFLGLFHLEIVQERLEREYDVDLIATSPSVEYRVITQKGDEVAIDNPA
                     MLPDPGTIQEIREPWMRVTIIVPTSYVGPIMDLAQNKRGTLMDMSYVDPTRAMLKYDM
                     PLSEVIVEFYDQLKSRSQGYASLDYELAEYRAADLVRLDILVSGTLVDALSVIVHRER
                     AQFMGRILTAKLRELIPKQMFDVRIQAAVGSKVIASETVKAKRKDVLAKCYGGDITRK
                     KKLLEKQKEGKKRMKMVGSVEIPQEAFLAVLRIKDEG"
     gene            2337..2834
                     /locus_tag="VFV20_03965"
     CDS             2337..2834
                     /locus_tag="VFV20_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287535.1"
                     /translation="MKQPRLQFEHPAQAAASAFLRAVLAGESLAVWAALSRETRGILQ
                     GLYAAHAGVSLRTASGIELDGTDERLGSVLAPLRASVLTALGEERIGGYGVSNARLVD
                     RQTAYVLLLPDFGEERVVSEDEWKPSHLLAFIHESHEWVVDLAKTAELSADAELPDPL
                     GQIRR"
BASE COUNT          466 a         1040 c          983 g          431 t
ORIGIN      
        1 cggaacgcgc gcgtggcgcg gcgcgtccgc tcccccgcga cgatcgcacc cggctcggcg
       61 ctctgctgca accgcgcggc cacgttcacc gcgtcgccgg tcacgaggaa gtccccggca
      121 ccggcctctc gggtggccac gacctcgccc gtgttcaccc cgatgcggag cacgagcccg
      181 gccgtcgcgg gatcggcggc gacccgttcg cggagcgcca gcgccgcggc cagcgcgcgc
      241 tcggcgtcgt cgccgtgcgc ctgggggatg ccgaacaccg ccatcaccgc gtcgccgatg
      301 aacttctcga gcgtcccgcc gtgcgccgcg accacgtcct ttgcgatcgc gtaatagcgg
      361 ccgagaaggg cgcggacgtc ttcggggtcg gtcgattcgc cgagcgcggt cgagccggcc
      421 acgtcggcga acagcacgct gacgacgcga cgctcctccg gcatggacgg attctcgcgg
      481 gcgaatggct gcgacgccag ccgctcgctg cccataggct tgaacgagat ggccgatcca
      541 cagacccgca tccgcaactt ctgcgtcatc gcccacgtcg accacggcaa gacgaccctc
      601 tcggacaggc tcatcgagct gaccaagacc gtcccgctgc ggcagatgcg tgcgcagatg
      661 ctcgactcca tggagctgga gcgcgagcac ggcgtcacca tcaaggcccg cgcggtgcga
      721 atggcctggc ccggacagga cggcgagatc tacgagctga acctcatcga cacgcccggc
      781 cacgtcgact tcaactacga ggtgagccgt tcgctcgcgg catgcgaggg cgcgctcctg
      841 ctcgtggacg cgtcgcaggg catcgaggcg cagacgctcg cgaacgcgta ccttgccgtc
      901 gagcaggggc tcgagatcat ccccgtgatc aacaaggtcg atctgcccgc catcgatctc
      961 gacgtcgttc gcgaggacct ccggacgacg ctcggcttca aggatgagga gatcctcctc
     1021 gcctccggga agaccggcct cggcgtcgag gacctgctca atgcgatcgt cgaccgcgtg
     1081 ccgccgccga gcggcgacgc ggcggcgccg ctgcgcgcgc tcatcttcga ttcccactac
     1141 gacacgtaca agggcgtggt ggcgcacgtc cgcgtggtcg acggctccct cgccgccgag
     1201 gaccgcatcc tcggcatggc gacgcagaag tcgagcgagc tgctcgagct cggcgtgttc
     1261 gcgcccgacg ccgtgccggt gcagcgactc gaggccggcg aggtgggcta cgtcgcgacc
     1321 gggctcaaga ccgtcgccga ctgccgggtc ggcgacacgc tcacgctgga ggcgcggccc
     1381 gcggccgagc cgctccccgg ataccgcccg gccaagccga tggtgttcgc cggtttctac
     1441 ccgcagcgcg ccgaggacta cccggagctg cgggagtcgc tcgagaagct gcgcctcaac
     1501 gacgcgtccc tggtgtacga gccggagacc tcgcaggccc tgggcttcgg cttccgatgc
     1561 ggcttcctcg gcctgttcca tctcgagatc gtgcaggagc gcctcgagcg cgagtacgac
     1621 gtcgacctca tcgcgacctc gccgtcggtc gagtaccgcg tgatcacgca aaagggcgac
     1681 gaggtcgcga tcgacaaccc cgcgatgctg ccggaccccg gcacgatcca ggagatccgc
     1741 gagccgtgga tgcgcgtgac gatcatcgtg ccgacgagct acgtcggtcc gatcatggac
     1801 ctggcgcaga acaagcgcgg gacgctcatg gacatgtcgt acgtcgatcc gacgcgcgcg
     1861 atgctgaagt acgacatgcc gctctcggaa gtgatcgtgg agttctacga ccagctgaag
     1921 agccgctccc agggctacgc gtcgctcgac tacgagctcg ccgagtaccg cgccgcggac
     1981 ctcgtgcgcc tggacatcct cgtgtccggc acgctcgttg acgcgctctc cgtcatcgtg
     2041 caccgcgagc gcgcccagtt catgggccgc atcctcaccg cgaagctgcg tgagctcatc
     2101 ccgaagcaga tgttcgacgt ccgcatccag gccgccgtgg gaagcaaggt gatcgcgagc
     2161 gagaccgtga aggcgaagcg caaggacgtg ctcgccaagt gctacggcgg cgacatcacg
     2221 cgcaagaaga agctgcttga gaagcagaag gaaggcaaga agcgcatgaa gatggtggga
     2281 tcggtcgaga tcccgcagga agcgttcctt gccgtgctgc gcatcaagga cgagggatga
     2341 agcagcccag gctgcagttc gagcaccccg cgcaggcggc ggcatcggcg ttcctccgcg
     2401 cggtgctcgc gggcgaatcg ctcgcggtct gggccgcgct ctcgcgcgag acgcgcggga
     2461 tcctccaggg tctctacgcc gcccatgccg gcgtctcgct tcgcaccgcc tcgggcatcg
     2521 agctggacgg cacggacgag cgtctcggct ccgtcctcgc gccgctgcgc gcctcggtcc
     2581 tcaccgcgct cggggaggag cggatcggcg gctacggcgt ctcgaatgcg cggctcgtcg
     2641 atcgccagac cgcgtatgtc ctgctgctgc cggacttcgg cgaggagcgc gtcgtcagcg
     2701 aggacgaatg gaagccctcg cacctgctcg cgttcatcca tgagtcgcac gagtgggtcg
     2761 tcgatctcgc gaagaccgcc gagctctccg ccgacgccga gctgccagat ccgctcggac
     2821 agatacggcg atgagcgccg tcgcgggccg cagctcggcg gaacgcgcgc tctcgctcta
     2881 cgtgcacatc cccttctgcg ccgcgcgctg tccctactgc
//
LOCUS       DASTZR010000179         5063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_87426, whole genome shotgun sequence.
ACCESSION   DASTZR010000179 DASTZR010000000
VERSION     DASTZR010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87426"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VFV20_03970"
     CDS             <1..580
                     /locus_tag="VFV20_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287536.1"
                     /translation="KLVAVPETVRAFRARGTRAELSALAARVHAARLPLDALVLDTSA
                     EAPVLLARAAGLAAAVARMGAALGALGRFTDADPSELARVHERTIREPTVLRLSLPPG
                     REVDITSGVARTTVHLGSGIAFAYELAGTDAVRALRRTAESAGGALVVERADPRLRSA
                     VGTWGAPRMPGEVARRLKERFDPRGTLAPGRM"
     gene            604..1872
                     /locus_tag="VFV20_03975"
     CDS             604..1872
                     /locus_tag="VFV20_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEU5287537.1"
                     /translation="MTVAPLAFDTEPARACIHCGMCLEACPTYLVTRLETESPRGRIH
                     LMQHLADGGSPTAALRGHLDRCLACRACEVACPSGVGYGALIENARAALDGASEASRS
                     PVASLARRLAFGLFAHPGRLELVAGVLALYERTGLRSLVHRTGLRAVLPRGLRRLEAM
                     YPPLTRPRYRPPASAPASPSARVALLLGCVMRVAYGDVHTAAARVLQRLGVEVIDAPS
                     QVCCGALHAHTGARAEAIALAKTNIAAFESAGVDAVVVDAAGCGSHLKGYAHLLAGDP
                     VWGARAEAFSAKVRDVTEYLSSLGGGSLGTLDLRVTYQEPCHLAHAQRIRAEPRALLS
                     RVRGLELVEMAESDVCCGSAGSYNITQPALADALLERKLAAIEATHADAVVSANPGCM
                     LQVQSGLQARGSRLPVLHVVEVLDRALARP"
     gene            1869..2321
                     /locus_tag="VFV20_03980"
     CDS             1869..2321
                     /locus_tag="VFV20_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287538.1"
                     /translation="MRRALTIAATVVATIVAVGAFLLLPASILVGHPFSAVIELVLLV
                     ACIWFVARVMRADRRQRLAAEAALPPERRPQRRQPRRPITFQLRETLVTFAVWFALIF
                     AFDWLALGMDPVVNLGVALFAGFMLATLTVTGRHMMFRLTAEEDEGKS"
     gene            2318..3253
                     /locus_tag="VFV20_03985"
     CDS             2318..3253
                     /locus_tag="VFV20_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEU5287539.1"
                     /translation="MNLGVQIEPQFGFTYEAIRSIARAAHGAGFTRLWVSDHLLLNAG
                     AVATDCLEAWTVLAALARDTEGIRIGPMVSCQSYRNPALLAKIAAGVDQLSGGRLEFG
                     IGAGWKDVEYRAYGYDFPEAGVRVTQLVETIEICTRLWRDERATYRGKHYRVEEAVCS
                     PKPKQTPLPIWIGGSKPRVMRIAARHAAWFNMTILAGSLEHRVSQMREGLEAACAAVG
                     RDAATLHRSLFVQAFVASTRAELDELVGELAARAKTTPDQWRAARAGAIIGTIDEAGE
                     RFRMLARSGIEHANVMLPYGHELDGVKHLSEVARA"
     gene            complement(3404..3838)
                     /locus_tag="VFV20_03990"
     CDS             complement(3404..3838)
                     /locus_tag="VFV20_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEU5287540.1"
                     /translation="MTRGPRAIAIAIFRRRDEILVSAVPDEVKGVTGWRAPGGGIEFG
                     ERGADTVTREVREELGVDVADVRYIGTVENVFTYLGRPGHEIVRVYAAAFADASLYER
                     ERFECVEEVSGPFVCVWKPLGEFGGRAPLYPEGVLELLRGRL"
     gene            complement(3835..>5063)
                     /locus_tag="VFV20_03995"
     CDS             complement(3835..>5063)
                     /locus_tag="VFV20_03995"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEU5287541.1"
                     /translation="PLLGVPYGAKDLLAAKGAPTTWGAPPYRDQVFDTDATAIARLVR
                     AGGVLAAKLAMVELAGGGGYRYPSASLQGPGRNPWDPGRWSGGSSSGSAAAVGAGIVP
                     FALGSETSGSIGTPSAYCGVTGLRPTYGLVSRHGAMALSWTLDKIGPMARTADDVAVV
                     LAAIAGPDRKDATSSGKRFRPLEGRPAAAAVRSARVAFAEEDVIDHASAEARAALERG
                     VEEFRRIVPAFHRATLPADLPYGPMVGTVYGAEGATIFAELIEGERFEELVDARQKAG
                     LRAALDIRARDYLQAMRLRVRLSAAFDEIFRDADAILSVGRAITATPITQALDSPPTT
                     ASNPPQTRRPGNLGLIPAGNLAGLPALFFPCGFASDGLPVGLQLVGRPFSEPLLVALA
                     SAYQRQTDHHTKRPAV"
BASE COUNT          673 a         1871 c         1787 g          732 t
ORIGIN      
        1 caagctcgtc gcggtgccgg agaccgtccg cgcgttccgc gcgcgcggca cgcgcgcgga
       61 gctgtccgcg ctcgcggcgc gggtgcacgc cgcgcgcctc ccgctggacg cgctcgtcct
      121 cgacacgtcg gccgaggcac ccgtgcttct ggcgcgcgcc gcaggcctcg cggcggccgt
      181 cgcacggatg ggtgcggcgc tcggcgcgct cggccggttc accgacgcgg acccatccga
      241 gctcgcccgc gtgcacgagc gcacgatccg cgaaccgacg gtgctccggc tctcgctgcc
      301 gcccggccgc gaggtcgaca tcacctccgg cgtagcgcgc accaccgtcc atctcggctc
      361 cggtatcgcg ttcgcgtacg agctggccgg taccgacgcg gtgcgcgcgc tgcggcgcac
      421 agcggagtcc gccggtggcg cgctcgtcgt cgagcgcgcg gatccgcggc tgcgatcggc
      481 cgtgggcaca tggggagcgc cgcggatgcc cggcgaggtc gcacgccggc tcaaggagcg
      541 gttcgatcca cgcggcacgc tcgcacccgg gcggatgtga atctcatcgc ttagcgttga
      601 cgggtgaccg tcgctcccct cgcattcgac accgagccgg cgcgtgcgtg catccactgc
      661 gggatgtgcc tcgaggcctg cccgacatac ctggtcacgc gtctggagac cgagtcgcca
      721 cgcgggcgta tccacctcat gcagcacctg gcggacggcg ggtcgccgac cgcggcgctg
      781 cgcgggcatc tggatcgctg cctggcgtgt cgcgcctgcg aggtcgcctg cccgtcgggc
      841 gtcggctacg gggcgctgat cgagaacgcg cgcgcggcgc tcgacggcgc gagcgaggca
      901 tcgcgctcgc cggtcgcatc gctcgcacgg cgcctcgcct tcggtctctt cgcgcaccct
      961 gggcggctcg agctcgtcgc cggcgtgctc gcgctgtatg agcgcaccgg ccttcgctca
     1021 ctcgtccacc gcaccgggct gcgcgcggtc ctgccgcggg gcctccgtcg cctcgaggcc
     1081 atgtacccac cgctcacgcg cccgcgctac cggccacccg catccgctcc cgcgagtccg
     1141 tccgcgcgcg tcgcgctcct cctcggctgc gtcatgcgcg tcgcgtacgg cgacgtccac
     1201 accgcggcgg cgcgcgtcct ccagcggctt ggcgtcgagg tgatcgacgc gccgtcccag
     1261 gtctgctgcg gcgccctgca cgcccacacc ggcgcgcgcg ccgaggcgat cgcgctggcg
     1321 aagacgaaca tcgccgcctt cgagtccgcc ggcgtcgacg cggtcgttgt agacgcggcc
     1381 gggtgcggat cgcacctcaa ggggtatgcc cacctcctcg ccggcgatcc ggtgtggggc
     1441 gcgcgtgccg aggcgttcag cgcgaaggtg cgcgacgtga ccgagtacct gtcgtcgctc
     1501 ggcggcggat cgctcggtac gctggacctc cgcgtgacct accaggagcc ctgccatctc
     1561 gcccacgcgc agcgcatccg cgcggagccg cgcgcgctgc tgtcgcgcgt gcgcgggctc
     1621 gagctggtgg agatggcgga gagcgacgtc tgctgcggga gcgcgggctc ctacaacatc
     1681 acgcagccgg cattggcgga cgcgctgctc gagcgcaagc tcgctgcgat cgaggcgacg
     1741 cacgccgacg cggtcgtgag cgccaatccc ggctgcatgc tccaggtgca gagcgggctg
     1801 caggcacgcg gctcgcgcct gccggtgctg cacgtggtcg aggtcctcga ccgggcgctg
     1861 gcacgaccgt gaggagagcg ctcacgatcg cggcgaccgt cgtggcgacg atcgtcgcgg
     1921 tcggcgcgtt cctgctgctc ccggcgtcga tcctcgtcgg ccacccgttc agcgccgtca
     1981 tcgagctcgt gctgctcgtg gcctgtatct ggttcgtcgc gcgcgtcatg cgggcggacc
     2041 gcaggcaacg actcgccgcc gaggccgcgc tcccacccga gcggcgtcca cagcggcggc
     2101 agcccagacg cccgatcacg ttccagctcc gcgagacgct ggtcacgttc gctgtttggt
     2161 tcgcgctcat cttcgcgttc gactggctcg cgctcggcat ggatcccgtc gtgaacctcg
     2221 gcgtcgcgct cttcgcgggc ttcatgctcg ccacgctcac cgtcacgggc cgccacatga
     2281 tgttccgcct caccgcggag gaggacgagg gcaagtcgtg aatctgggcg tccagatcga
     2341 gccgcagttc gggttcacct acgaggcgat ccgcagcatc gctcgcgccg cccatggcgc
     2401 cggcttcacg cgcctctggg tctccgacca tctgctgctg aacgccggtg cggtcgcgac
     2461 cgactgcctg gaggcatgga ccgtcctggc ggcgctcgcg cgtgacacgg agggcatccg
     2521 catcggcccg atggtcagct gccagtccta tcggaacccg gcgctgctcg cgaagatcgc
     2581 ggccggcgtc gaccagcttt ccggagggcg gctcgagttc ggcatcggcg cgggctggaa
     2641 ggacgtcgag taccgcgcgt atggctacga cttcccggag gccggcgtgc gcgtcacgca
     2701 gctggtcgag acgatcgaga tctgcacgcg cctctggcgc gacgagcgcg cgacctaccg
     2761 cggaaagcac taccgcgtcg aggaggccgt ctgctcgccc aagccgaagc agacgccgct
     2821 tcccatctgg atcggcggct cgaagccgcg cgtgatgcgg atcgcggcca ggcacgcggc
     2881 gtggttcaac atgacgatcc tcgccggatc gctcgagcat cgcgtcagcc agatgcgcga
     2941 aggcctggag gccgcgtgcg ccgccgtcgg ccgtgacgcc gcgacgctgc accgcagcct
     3001 cttcgtgcag gccttcgtcg catcgacgcg cgccgagctc gacgagctcg tcggcgagct
     3061 cgccgcgcgc gcgaagacca caccagatca gtggcgcgcg gctcgcgccg gcgcgatcat
     3121 cggcaccatc gacgaggcgg gcgagcgctt tcggatgctc gcgcgctccg gcatcgagca
     3181 cgcgaacgtg atgctcccct acggccacga gctcgatggt gtcaaacacc tttccgaggt
     3241 cgcgcgcgcc tagactcgct cgcgccgaca aggggtcgag cccgaggaga gcgcgacgga
     3301 tggaagtgtg gttcgcggtc agcgtgatcg cgccgctctg gatcctcggc gggtacgcgg
     3361 cgctcctatt cttcgatcct tcacggggtt ggcgccgccg gcgttagaga cgccccctca
     3421 gcagctcgag gacaccctcc ggatagagcg gcgcgcggcc accgaactcg ccgagtggct
     3481 tccacacaca gacgaaggga ccgctcacct cctcgacaca ctcgaagcgc tcgcgctcgt
     3541 agaggctcgc gtcggcgaat gccgcggcgt acacgcggac gatctcgtga ccgggccggc
     3601 cgagatacgt gaagacgttc tcgaccgttc cgatgtagcg cacgtccgcc acatcgacgc
     3661 ccagctcctc gcggacctcg cgcgtgaccg tgtccgcacc acgttcgccg aactcgatgc
     3721 cgccgccggg cgcgcgccat ccggtgacac ccttcacttc gtctgggacg gcggacacga
     3781 ggatctcgtc gcgtcgcctg aagatcgcga tcgcgatcgc gcggggtccg cgcgtcacac
     3841 cgctggccgc ttcgtgtggt ggtccgtctg gcgctggtag gcggatgcga gcgcgacgag
     3901 cagcggctcc gagaagggac gtcccacgag ctgcaacccc acagggagtc catcgctcgc
     3961 gaagccgcag gggaagaaga gcgcggggag gccggcgagg ttcccggcgg ggatgagccc
     4021 gaggttgccg ggacgcctcg tctgcggcgg attgctcgcg gtcgtgggcg gcgagtccag
     4081 ggcctgcgtg atcggcgtcg ccgttatcgc gcggcccacc gacaggatcg cgtctgcgtc
     4141 gcggaagatc tcgtcgaacg cggcggacag ccgcacgcgc aggcgcatcg cctgcaggta
     4201 gtcgcgtgcg cggatgtcca gcgcggcacg cagacccgcc ttctgccgcg cgtccaccag
     4261 ctcttcgaag cgctcgccct cgatgagctc cgcgaagatc gtcgcgccct ccgcgccgta
     4321 tacggtgccg accatcgggc cgtacgggag gtccgccgga agcgtggccc ggtggaacgc
     4381 cggcacgatg cggcgaaact cctcgacgcc acgctcgagc gccgccctcg cctcggcgga
     4441 cgcgtgatcg atgacgtcct cctccgcgaa cgcgacgcgg gccgatcgca ccgcggcggc
     4501 ggcggggcgg ccctcgaggg ggcggaaccg ctttcccgat gacgtcgcgt cctttcggtc
     4561 cggacccgcg atggccgcga gcacgaccgc gacgtcgtcc gcggtgcgcg ccatcgggcc
     4621 gatcttgtcg agcgtccacg agagcgccat cgctccgtgc cgcgacacga gcccgtacgt
     4681 cggacgcaga ccggtgacgc cgcagtaggc ggagggcgtt ccgatcgacc cggacgtctc
     4741 gctgccgagc gcgaacggca cgatgccggc gccgacggcg gcagccgatc cgctcgacga
     4801 gccgccggac cagcggccgg ggtcccacgg attccggccc gggccctgga gcgacgcgct
     4861 cgggtagcga tagcctccgc caccggcgag ctcgaccatc gcaagctttg ccgccaagac
     4921 accgcccgcg cgcacgagcc tcgcgatcgc cgtcgcgtcc gtgtcgaaga cctggtcgcg
     4981 atagggcggc gcgccccacg tcgtcggcgc acctttggcg gcgaggagat ccttcgctcc
     5041 atacggcaca ccgaggagcg gac
//
LOCUS       DASTZR010000180         3153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_766245, whole genome shotgun sequence.
ACCESSION   DASTZR010000180 DASTZR010000000
VERSION     DASTZR010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766245"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /locus_tag="VFV20_04000"
     CDS             complement(<1..1042)
                     /locus_tag="VFV20_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287542.1"
                     /translation="MLIRGELVLLLVGVVLALIAIASVFSARLARWRTPFVALACLMA
                     GLAPLLLYAFIDPAQARGTALWEWSAVGGPTIQASYRLDGLAALGLAIAVLYGAAALI
                     ATTRVTSRSALLRPTLLLNAFVLVTLVVTDDLIADTVVLGALAAITTFIALLVSPPSA
                     VARITAYLAAGVQAFIVAGLLVTRFGVPSFRFDAIPAGSMSPGVVLAAAVGGALFAGL
                     YPFVPWGYRQDESGERESLRGLLTMPAGVGATIVLIRVVGVTRIDISTLALPGIPPTW
                     LVVVAALVAAVAVWRTLRGHGRGRREAVVSVVALAILVLYPYLHWSHLVALACLLTVL
                     YAAAVSLAQPDHW"
     gene            1062..2507
                     /gene="rpoN"
                     /locus_tag="VFV20_04005"
     CDS             1062..2507
                     /gene="rpoN"
                     /locus_tag="VFV20_04005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEU5287543.1"
                     /translation="MDLRLLPQQQLKVTPKQIAANYILQLSSMELQDVISQELQENPA
                     LDLEEATVCPLCGEPLQGRVCLNCFGLASKAPRNDEPDSEPLEGSSLMQRDEDDEEFD
                     PIVRAEAEQTIAEYLSWNVRVIVPARLHHVAEYLIGNINDNGRLEGVTLEDAAKTCNV
                     PLEDVEQVHRAILSIEPWGIGARDTRECLLVQMEHLVEIGEHVPPHARQIVAEQLRAL
                     GEHKYGDIAQALRISREEVQEASDYIKDVLNPFPAHAFTAGPGGTSIGGREVGLRPDV
                     MILKDESGDFKVEVIESRRFSLRVNPVYRQLVAQVHAARREGGGESVPMSDQDRQHIR
                     DYVTRAKFFIDNINQRRQTIARITEVIVECQKEFLESGIQSLRPLTRAEVGERIGMHE
                     STVSRATAGKFVLLPSGQVVAFSVFFTASLGVKDVIKQIIENEDRTRPYSDQEIVEKL
                     RNDNGIILARRTVAKYREELQILPARLRKHV"
     gene            complement(2515..2781)
                     /locus_tag="VFV20_04010"
     CDS             complement(2515..2781)
                     /locus_tag="VFV20_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287544.1"
                     /translation="MPPQKASKSQPKADASEKAKSKDREWLTTAELATKIGWREHALR
                     DAMRGKDLPGVRRTAFGYHIESSSVDQIKAALGEPPAKPNAARR"
     gene            2902..>3153
                     /locus_tag="VFV20_04015"
     CDS             2902..>3153
                     /locus_tag="VFV20_04015"
                     /inference="COORDINATES: protein motif:HMM:NF015019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEU5287545.1"
                     /translation="MEGFLGASPLLWVVPLSGLLAVVVAAYFAWDVLRSDTGTPAMQK
                     VAGAIYTAAVAFIKRQYRTIALLALGGMVVIGLVIYFFES"
BASE COUNT          566 a         1029 c         1086 g          472 t
ORIGIN      
        1 gccagtgatc gggctgagcg agggagaccg cggccgcgta gaggacggtg agaagacacg
       61 ccagcgccac gagatggctc cagtgcagat atggatagag gaccaggatc gcgagcgcga
      121 ccaccgacac caccgcctcc cggcgaccgc gaccgtggcc acgcagcgtg cgccacacgg
      181 cgaccgcggc cacgagggcg gcgacgacca cgagccacgt cgggggtatg cccggcagcg
      241 cgagcgtcga gatgtcgatg cgcgtgacac cgacgacgcg gatgagcacg atggtcgcgc
      301 cgacgccggc cggcatcgtc agcagtccgc gcaggctctc gcgctcaccg ctctcgtcct
      361 gccggtagcc ccacgggacg aacgggtaca gcccggcgaa gagcgcccct ccgaccgccg
      421 cggcaaggac gacgcccggg ctcatcgagc cggcggggat cgcgtcgaag cggaatgacg
      481 ggacaccgaa gcgcgtgacg agaagtcccg cgacgatgaa tgcctggacg ccggcggcga
      541 gatacgccgt gatgcgcgcg accgcggacg gcggtgacac gaggagcgcg atgaaggtgg
      601 tgatcgccgc gagcgcgccg agcacgactg tgtcggcgat gaggtcgtcc gtgacgacga
      661 gcgtgacgag gacgaaggca ttgaggagga gcgttggtcg cagcagcgcg gatcgcgacg
      721 tcacgcgtgt cgtcgcgatc agcgcggccg caccgtacag gacagcgatg gcgaggccga
      781 gcgccgcaag tccgtccaac cgatacgaag cttggatggt cggtcctccg acggccgacc
      841 attcccacag ggccgttcca cgggcctgcg cggggtcgat gaaggcgtac aagagaagtg
      901 gcgcaagtcc tgccatgagg caggcgagcg cgacgaatgg tgtgcgccat cgcgcgagac
      961 gagcggaaaa tacggaggcg atcgcgatga gcgcgagcac cacgccgacg agcaataaga
     1021 cgagctcgcc gcgtatgagc attggggtaa ggtagccgat agtggatctg cgactccttc
     1081 cgcaacagca gttaaaggtc acgccgaaac agatcgccgc gaactacatc cttcagctgt
     1141 cctcgatgga gctgcaggac gtcatcagtc aagagctcca ggagaatccc gccctcgatc
     1201 tcgaagaagc gacggtctgt ccgctgtgcg gcgagccgct ccagggtcgg gtctgcctca
     1261 actgcttcgg cctcgcgtcg aaagcgccgc gcaacgacga gcccgactcc gagccgctcg
     1321 agggatcgag cctcatgcag cgcgacgagg acgacgagga gttcgatccg atcgtgcgtg
     1381 ccgaggcgga gcagaccatc gccgagtacc tgtcgtggaa cgtgcgtgtc atcgtgcccg
     1441 cgcgactgca tcacgtcgcc gaatacctca tcggcaacat caacgacaac ggcaggctcg
     1501 agggcgtcac gctcgaggac gccgcgaaga cctgcaatgt tccactcgag gacgtggagc
     1561 aggtccaccg cgcgatcctg tccatcgagc cgtggggcat cggcgcgcgc gacacgcgtg
     1621 aatgtctcct ggtccagatg gagcatctcg tggagatcgg cgagcacgtc cccccacacg
     1681 cgaggcagat cgtcgcggag cagctgcgcg cgctgggcga gcacaagtac ggcgacatcg
     1741 cccaggcgct gcgcatcagc cgcgaggaag tgcaggaggc gtccgactac ataaaggacg
     1801 tgctcaatcc gttcccggcg cacgcgttca ccgcgggccc gggcggcacg agcatcggcg
     1861 ggcgcgaggt cggcctgcgc cccgacgtga tgatcctcaa ggacgagagc ggcgacttca
     1921 aggtcgaggt catcgagtca cggcgcttct cgctgcgggt caaccccgtc taccggcagc
     1981 tcgtcgcgca ggtgcatgcc gcgcggcgcg agggcggcgg cgagtcggtg ccgatgagcg
     2041 accaagaccg ccagcacatc cgcgactacg tgacccgcgc gaagttcttc atcgacaaca
     2101 tcaaccagcg acggcagacc atcgcgcgca tcacggaggt catcgtcgaa tgtcagaagg
     2161 agttcctcga gagcggcatc cagtcgctgc gtccgctgac gcgggccgag gtgggtgagc
     2221 ggatcgggat gcacgagtcg accgtgagcc gcgcgaccgc agggaagttc gtgctcctcc
     2281 ccagcgggca ggtcgtggcg ttctccgtct tcttcacggc gtcgctcggc gtgaaggacg
     2341 tgatcaagca gatcatcgag aacgaggacc gcacgcggcc gtactccgat caggagatcg
     2401 tggagaagct gcggaacgac aacgggatca tcctcgcgcg ccggacggtc gcgaagtatc
     2461 gcgaggagct acagatcctg cccgcccgtc tccgcaagca cgtctagatc cggctcagcg
     2521 ccgggcggcg ttcggcttcg cgggcggctc gcccagggcg gccttgatct ggtcgaccga
     2581 cgacgactcg atgtggtagc cgaacgccgt gcgacgcacg ccgggaaggt ccttcccgcg
     2641 catcgcgtcg cggagggcgt gctcgcgcca cccgatcttg gtggcgagct cggcggtcgt
     2701 gagccattcg cggtccttgc tcttggcctt ctcggacgcg tcagccttgg gctggctctt
     2761 cgatgctttc tgtggcggca tgcatcgaac tctagcgggt ttgccgcctc ccgcgccgtc
     2821 gcctaaactc ggccgagagc gctgcggccc cctccgtcgg ggcgggcgct tgcttcttgt
     2881 ccattcattt atcagttttc ggtggagggc ttcttgggcg cgtcaccact cctttgggtc
     2941 gttccgcttt ccggactgct cgccgtcgtc gtcgccgcct atttcgcctg ggacgtgctg
     3001 cgatctgaca ccggcacgcc ggcgatgcag aaggtggccg gcgccatcta cacggcggcg
     3061 gtcgcgttca tcaagcgcca gtaccgcacc atcgcgctcc tcgcgctcgg cggcatggtc
     3121 gtcatcgggc tcgtcatcta cttcttcgag tcc
//
LOCUS       DASTZR010000181         3194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_741835, whole genome shotgun sequence.
ACCESSION   DASTZR010000181 DASTZR010000000
VERSION     DASTZR010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741835"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="VFV20_04020"
     CDS             <1..1170
                     /locus_tag="VFV20_04020"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5287546.1"
                     /translation="FLPLLIQDLGVTDRGEVALWTGLAIGTSGMAMALASPVWGLLAD
                     RFGRKAMLVRAIASGSILLGLQAAVTSVWQLAIVRILQGAFTGTQTAAAMLLAGIVPK
                     ERTGFALGMLNTAVQVGNLAGPVLGGIAVASIGLRGSFVIGALVLGVCTVITIVWTDD
                     VAVAPTSSGTGVRNVARDVFTPFAWPGLRGVLVIGTMVQVAWAGTIALIAIYVQDLAR
                     PSWLSLELTIGLSLALTALAAAIAMPILGAWADRHDPRALLVVSLVVVSLSLVPQALI
                     PDAVVFLALRLVLGAGAAGTTSAIAVLTRAGAPVGGEGRAFGALAAAQNLGWGIGPIV
                     GASFAAIVGIPALYLAAAVITLALVPAALTRSWFATPERMRAEPLPIVTTAPAED"
     gene            1199..1519
                     /locus_tag="VFV20_04025"
     CDS             1199..1519
                     /locus_tag="VFV20_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287547.1"
                     /translation="MTDARRQLIKVGLGPEPTGRWAKLRHRLNEVLWGFFVYEFWHGL
                     HEERSRYADALNVLMMGELLGIPLMSSTVTLRLLPHMLPDLKKWKERQAAEAEILERA
                     PEIH"
     gene            1519..2049
                     /locus_tag="VFV20_04030"
     CDS             1519..2049
                     /locus_tag="VFV20_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287548.1"
                     /translation="MDQSTGVLLVFVLAFVFVGWYIVGGLWQRRIAGRYVRALGLVAQ
                     QLSESKAMPRMRWLGQAGFQLTVDDAVAPFTKLSIVTLLKPREAVALWLAAVLRRRGD
                     SVVMRADLRERPQGAGDATPAAPLTQLSFARESPHVIAAIDPRSLRGQTAEAQAASIQ
                     RAVRDASARSGSVRST"
     gene            2080..3114
                     /gene="fgd"
                     /locus_tag="VFV20_04035"
     CDS             2080..3114
                     /gene="fgd"
                     /locus_tag="VFV20_04035"
                     /EC_number="1.1.98.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014446521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase
                     (coenzyme-F420)"
                     /protein_id="HEU5287549.1"
                     /translation="MLKVGYKASAEQFDARTLLRFAVLAEDAGFDSIAVSDHFQPWRH
                     HGGEAPNAFVWLGAAAASTKRAELGTSVATPTFRYHPALVAQAAATIESLAPGRFFLG
                     IGTGESMNEVPLGIEWPEIKERFARMREAVDLMRKLWSEEFVTFEGQYYKTKNATIYE
                     RPESGVVPLYIAATGEAAARLAGRVAQGFICTSGKGEELYTKTLIPAVVEGAKKAGRD
                     VRELDMTIEVKVSYDTDRERAMADTMHWAALALPGEQKVGVEDPREMERLASALTPEQ
                     AASRWIVSIDPDEVAERVGAYVRMGFNHVVIHGPGPDQERFVRLFSKDVMPRLRAKYG
                     DVRPAAATTA"
BASE COUNT          414 a         1160 c         1154 g          466 t
ORIGIN      
        1 ttcctgccgc tcttgatcca ggacctcggc gtcaccgacc ggggagaggt cgcgctctgg
       61 accggcctcg cgatcgggac gagcggcatg gccatggccc tcgcgtcgcc ggtgtggggc
      121 ctcctcgccg accgcttcgg gcgcaaggcg atgctcgtgc gcgcgatcgc gtcagggtcg
      181 atcctcctcg ggctccaggc cgcggtgacg agcgtgtggc agctcgcgat cgtgcgcatc
      241 ctgcagggcg cgttcaccgg gacgcagacc gcggccgcga tgctgctcgc gggcatcgtc
      301 cccaaggagc gcaccggttt cgcgctcggc atgctgaaca ccgcggtgca ggtgggcaat
      361 ctcgccggtc ccgtcctcgg cgggatcgcc gtggcgagca tcgggctgcg cggctcgttc
      421 gtcatcggcg cgctcgtcct cggcgtctgc accgtgatca cgatcgtgtg gaccgacgac
      481 gtcgcggtcg cgcccacgtc ctccggcacg ggcgtgcgga acgtggcgcg cgacgtgttc
      541 acgccgttcg cgtggcccgg actgcgcggt gtgctcgtca tcggcacgat ggtccaggtc
      601 gcgtgggccg gcacgatcgc gctcatcgcc atttacgtgc aggacctcgc gcggccgtcg
      661 tggctgagcc tcgagctcac catcggcctc tcgctcgcgc tcaccgcgct cgcggcggcg
      721 atcgccatgc cgatcctcgg agcctgggcc gaccgccacg atccgcgcgc gctcctcgtg
      781 gtgtcgctcg tcgtcgtctc gctctcgctc gtgccgcagg cgctcatccc cgacgcggtc
      841 gtcttcctcg cgctgcgcct tgtgctcggc gcgggcgccg ccgggacgac gtcggccatc
      901 gcggtgctca cgcgcgccgg cgcgcccgtc ggcggcgagg gccgcgcgtt cggcgcgctc
      961 gccgccgcgc agaacctcgg ctggggcatc gggcccatcg tcggcgcctc gttcgccgcg
     1021 atcgtgggca tccccgcgct ctatctcgcg gccgcggtca tcacgctcgc gctcgtgccg
     1081 gcggcgctca cgcgctcgtg gttcgcgaca ccggaacgca tgcgcgccga gccgctgccg
     1141 atcgtgacga ccgcccccgc ggaggactga gcccgcggcc aggggctagg cttcgcacgt
     1201 gaccgacgcg cggaggcagc tcatcaaggt gggcctcggc cccgagccaa cgggccgctg
     1261 ggcgaagctg cggcaccggc tcaacgaggt gctctggggc ttcttcgtgt acgagttctg
     1321 gcacggcctg cacgaggagc gctcgcgcta cgccgacgcg ctgaacgtcc tcatgatggg
     1381 cgagctcctg ggcatcccgc tcatgagctc caccgtcacg ctgcgcctgc tcccccacat
     1441 gctccccgac ctgaagaagt ggaaggagcg ccaggccgcg gaggccgaga tcctcgagcg
     1501 cgcgccggag atccactgat ggatcagagc accggcgtcc tcctcgtctt cgtcctcgcg
     1561 ttcgtgttcg tcggctggta catcgtcggc gggctctggc agcggcgcat cgcgggccgc
     1621 tacgtgcgcg cgctcggcct cgtggcgcag cagctctccg agagcaaggc catgccgcgc
     1681 atgcgctggc tcgggcaggc cggcttccag ctcaccgtcg acgacgcggt cgcgcccttc
     1741 accaagcttt ccatcgtgac gctgctgaaa ccgcgcgagg ccgtcgcgct gtggctcgcc
     1801 gcggtgctgc gccggcgcgg cgacagcgtg gtcatgcgcg cggacctgcg cgagcggccg
     1861 cagggcgccg gggacgcgac gccggcggcg ccgctgacgc agctctcgtt cgcgcgcgag
     1921 tcgccgcacg tcatcgcggc gatcgacccg cgttcgctca gggggcagac ggccgaggcg
     1981 caggcggcga gcatccagcg cgcggtgcgc gacgcatcgg cgcggagcgg gagcgtccgc
     2041 agcacctaga ctcgacgcga attccgaatg aggtgggaaa tgctcaaggt cggctacaag
     2101 gcgtccgctg aacagttcga cgcgcggaca ctgctgcgct tcgcggtgct cgcggaggac
     2161 gccgggttcg acagcatcgc cgtgagcgac cacttccagc cgtggcgcca ccacggcggc
     2221 gaggcgccca acgcgttcgt gtggctcggc gcggccgccg cgtcgaccaa gcgcgcggag
     2281 ctgggcacga gcgtcgcgac gccgacgttc cgctaccacc ccgccctcgt cgcgcaggcc
     2341 gcggcgacca tcgagtcgct cgcgcccggc cgcttcttcc tcggcatcgg caccggcgag
     2401 tcgatgaacg aggtcccgct cggcatcgaa tggcccgaga tcaaggagcg cttcgcgcgc
     2461 atgcgcgagg cggtggacct catgcgcaag ctctggagcg aggagttcgt gacgttcgag
     2521 ggccagtact acaagacgaa gaacgccacg atctacgagc ggccggagtc gggcgtcgtc
     2581 ccgctgtaca tcgcggcgac cggcgaggcc gccgcgcggc tcgcgggccg tgtcgcgcag
     2641 ggcttcatct gcacgagcgg caagggcgag gagctgtaca cgaagacgct catccccgcc
     2701 gtcgtcgagg gcgcgaagaa ggcgggccgc gacgtgcgcg agctcgacat gaccatcgag
     2761 gtgaaggtct cgtacgacac cgaccgcgag cgcgcgatgg ccgacacgat gcactgggcg
     2821 gcgctcgcgc tgcccggcga gcagaaggtc ggcgtggagg acccgcgcga gatggagcgc
     2881 ctcgcgtccg cgctcacgcc ggagcaggcc gcgtcgcgct ggatcgtgtc catcgatccc
     2941 gacgaggtgg ccgagcgcgt cggcgcgtac gtgcgcatgg gcttcaacca cgtggtcatc
     3001 cacgggcccg gcccggacca ggagcgcttc gtgcggctct tctccaaaga cgtcatgccg
     3061 cgcctgcgtg cgaagtacgg cgacgtgcgt cccgccgcgg ccacgacggc gtagccggag
     3121 cacgatgcgc ccgctcgacg tcgtcgcggg tcccgaggag ctcgcgcagg cggccgcggc
     3181 gatcgtcgcc gagc
//
LOCUS       DASTZR010000182         2572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1029006, whole genome shotgun sequence.
ACCESSION   DASTZR010000182 DASTZR010000000
VERSION     DASTZR010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029006"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="VFV20_04040"
     CDS             complement(<1..1065)
                     /locus_tag="VFV20_04040"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5287550.1"
                     /translation="MRKDLRAGTRVVRSARSLPAGEGIPVAPPLVQSVAFDFGSAERQ
                     DEVFAGDRSGYVYGRYGSPTTAALETTLAELEGAEAAVCFTTGMAAIAAYVDACAVAH
                     GGRVVAQEDIYGGTRALFERWVSERGAKIVFVDATDQSAVERALASEPTTLLYIEAIA
                     NPLLRVADVAALARIARARGVTMAIDATFATPLLVRPLSLGADVVIHSLTKYLNGHGD
                     VMGGSVAGSTELAKAMRDRTILDGAYLPPHEAWLCIRGMRTLELRVRRQCDTALRVAR
                     HLAAHPKIARVHYPGLESHPQHALAEKQFGGRYGGVVTFDLEDGTRAGAFRFLDALEL
                     AASATTLGDLFSEVLYPAISS"
     gene            complement(1160..1510)
                     /locus_tag="VFV20_04045"
     CDS             complement(1160..1510)
                     /locus_tag="VFV20_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein B"
                     /protein_id="HEU5287551.1"
                     /translation="MARRQGGGMTVAEAGRMGGRLVSEKYGPEFYEKIGKKGGSSTAS
                     KYGPEFFGRIGKKGGKAVTAKYGPGHFEKIGRKGGQKVADLIERAKSLEGGERAEKPE
                     RVEDQEREETAAGS"
     gene            complement(1614..>2572)
                     /locus_tag="VFV20_04050"
     CDS             complement(1614..>2572)
                     /locus_tag="VFV20_04050"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5287552.1"
                     /translation="RATIGELPLRIRVLFEGEEEIGSEHLAAFVSAHGDRLRADGCIW
                     EAGYKDAAGRPTISLGLKGIAYFELRVRGPKTDVHSSQGTIIPNPAWRLVWALGTLKD
                     ANDNITIDGLMDHVRKPSAADVALLERLPYDEAEVKRIHGIGGFVRDLTGTPLKLKHF
                     FEPTCTICGLTSGYSGPGSKTVLPAVASAKLDFRLVPDLDPDLVARLLRAHLDRRGFG
                     DIEITPMHGEKPSRWPADSAVARAAVAACRDAYGTEPVVYPLMVGSGPMAQVCDALGI
                     PVVGFGSGNASSANHAPNENIRVADYIDHIRAFGRFLHTFAA"
BASE COUNT          373 a          917 c          863 g          419 t
ORIGIN      
        1 cgacgagatc gccgggtaga gcacctcgct gaagagatcg ccgagcgtcg ttgcggatgc
       61 cgcgagctcg agcgcgtcga ggaagcggaa cgcgccggcg cgcgtcccat cctccagatc
      121 gaatgtcacg acgccgccgt aacgtccccc gaactgcttc tcggcaagcg cgtgctgcgg
      181 gtgcgactcg agccccgggt aatggacgcg cgcgatcttc ggatgcgccg caagatgtcg
      241 cgccacgcgc agcgccgtgt cgcactgccg ccgcacgcgc agctccagcg tgcgcatgcc
      301 gcggatgcag agccaggctt cgtgcggcgg caggtacgcg ccgtcgagga tggtgcggtc
      361 gcgcatcgcc ttcgcgagct ccgtgctgcc ggcgacgctg ccgcccatga cgtcgccgtg
      421 cccgttgagg tacttcgtga gcgaatggat gaccacgtcc gcgccgagtg acagcggccg
      481 caccagcagc ggcgtggcga aggtcgcgtc gatcgccatc gtcaccccac gcgcgcgggc
      541 gatgcgcgcg agcgccgcca cgtcggcgac gcgcaggagc ggattcgcga tggcctcgat
      601 gtacagcagc gtcgtgggct cggacgcgag cgcgcgctcc accgcagact ggtcggtcgc
      661 gtcgacgaac acgatcttgg ccccgcgctc gctgacccag cgctcgaaga gcgcgcgcgt
      721 accgccgtag atgtcctctt gggcgacgac cctgccgccg tgcgcgaccg cgcatgcgtc
      781 gacgtaggcg gcgatcgccg ccatgccggt cgtgaagcac accgcggcct ccgcgccttc
      841 cagctccgcg agggtcgtct cgagcgcggc ggtcgtgggc gacccgtatc gcccgtagac
      901 gtagccgctg cgatcgcccg cgaagacctc gtcctgccgc tcggcggagc cgaagtcgaa
      961 tgcgacgctc tgcacgagcg gtggcgcgac cgggatcccc tcaccggcgg gcagcgatcg
     1021 cgcggaacgc acgacgcgcg tacccgcccg cagatccttc ctcattgcgg ccgagtctat
     1081 tcttccgtct cgttgtcact cgacgagctg gactcgctcc tcgcgagtgg atcgctcgtc
     1141 gctcgactcg actttgaccc taagacccgg ccgccgtctc ctcgcgctct tggtcttcga
     1201 cgcgttccgg cttctcggcg cgctcgccac cctcgagcga cttcgcgcgc tcgatgaggt
     1261 ccgcgacctt ctgaccgccc ttgcgtccga tcttctcgaa gtggcccggg ccgtacttcg
     1321 ccgtgacggc cttgccgccc ttcttgccga tgcgaccgaa gaactcgggt ccgtacttgg
     1381 acgccgtgct cgagccgccc ttcttcccga tcttctcgta gaactcggga ccgtacttct
     1441 cgctcacgag acgtccgccc atgcgacccg cctcggccac ggtcatcccg ccaccttgtc
     1501 tacgtgccat ggcctgctcc tttcgattcc gggacaggta gggggcaggc tgcgtgccgt
     1561 acaggtgtcc tctcggtcga cagaacgtaa cgacttcgca acgatgcgaa acgttaggcg
     1621 gcgaaggtgt ggaggaagcg tccaaacgcg cggatgtggt cgatgtagtc cgcgacccgg
     1681 atgttctcgt tgggcgcgtg attggcggac gacgcgttcc cggagccgaa gccgacgacc
     1741 gggatgccga gcgcatcgca cacctgcgcc atcggccccg agccgaccat cagcggatac
     1801 accaccggct ccgtcccgta ggcgtcgcgg cacgcagcaa ctgccgcgcg ggcgaccgcg
     1861 gagtcggcgg gccaccggct cggcttctca ccgtgcatcg gcgtgatctc gatgtcgccg
     1921 aagccgcggc gatcgaggtg cgcacggagc aggcgtgcga caaggtcggg atccaggtcg
     1981 ggcaccaggc ggaagtcgag cttcgcgctc gcgacggcgg ggagcacggt cttcgagccc
     2041 ggccccgagt acccggaggt gaggccgcag atcgtgcagg tcggctcgaa gaagtgcttc
     2101 agcttgagcg gcgtcccggt gaggtcgcgc acgaagccgc cgatgccgtg gatccgcttc
     2161 acctcggcct cgtcgtacgg cagacgctcg agcagcgcca catccgccgc gctcggcttt
     2221 cgcacgtggt ccatcaggcc gtcgatggtg atgttgtcgt ttgcgtcctt cagcgtgccg
     2281 agcgcccaca ccaggcgcca cgccgggttc gggatgatcg tgccctgcga cgagtgcacg
     2341 tcggtctttg gcccacgcac gcgaagctcg aagtacgcga tgcccttgag gcccaggctg
     2401 atcgtcggcc gtccggcggc gtccttgtac cccgcctccc agatgcaccc gtcggcgcgc
     2461 agccggtcgc cgtgtgccga cacgaaggcc gcgaggtgct cggagccgat ctcctcctcg
     2521 ccttcgaaca gcactcggat gcgcagcggg agctcgccga tcgtggcgcg at
//
LOCUS       DASTZR010000183         2094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_611179, whole genome shotgun sequence.
ACCESSION   DASTZR010000183 DASTZR010000000
VERSION     DASTZR010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611179"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VFV20_04055"
     CDS             complement(<1..717)
                     /locus_tag="VFV20_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEU5287553.1"
                     /translation="MCGVFGIRGHPDAASLTHLGLYSLQHRGQESAGIVAVGDDGTAR
                     NLRAMGLVSDKFSPESIAALTGSTAIGHTRYSTTGSSTIENAQPVLARFRDGHIALAH
                     NGNLTNSSELRFELEDQGSIFASTMDSEVIVHRMARSRSAAPEEKLADALRGVEGAYS
                     AVVLAGDTLLAARDPRGWRPLVMGRLNGAVIFASETCALDIVGATFEREIEPGEIVAV
                     DASGIRSSRPFPGVESRKCVF"
     gene            complement(722..>2094)
                     /locus_tag="VFV20_04060"
     CDS             complement(722..>2094)
                     /locus_tag="VFV20_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEU5287554.1"
                     /translation="ERMLVVAKRGHENAVAEILQRWDLTAAVIGEVIAEPVYRVTEGD
                     TVVAEFPGSRLVTDCPRYSPEARESDAIVRLRETDVASIPELPEEADPLWTLERMLSS
                     PTIASKAWAYRQYDSTVRTNTVIGPGGDAAVLRLRGSNKAIAVKTDCNGRYVYLSPRN
                     GARAAVCEAARNVACAGARPLAITNCLNFGNPRRPENFFQLSEAIAGMGEACRALDTP
                     VTGGNVSLYNESPSGAVYPTPVIGMLGLIDDIAHVTPAAWSVDGDVIVLLGEILGEPA
                     DGLGGSEYLARIHRTVAGAPPKCDPELERDTIACLLECIRAGHVRSAHDCSDGGLGVA
                     VAECAIANRSAVVGAEIDLSAWADVPLRELLYNEAQARIIVTVPESSLAEIARIAVKH
                     MVPPMVIGRVRTGSGNLEIEHGNNRALVAPLDRLAAAYHDAIPAIMEGSVESAAVLEQ
                     AAVTTV"
BASE COUNT          298 a          746 c          717 g          333 t
ORIGIN      
        1 gaagacgcac ttgcgcgatt cgacgccggg gaagggacgc gacgaccgga ttccgctggc
       61 gtccacggcc acgatctcgc cgggctctat ctcccgctcg aaggtcgccc cgacgatgtc
      121 cagcgcgcag gtctcggagg cgaagatcac ggcgccgttg agccgtccca ttaccagcgg
      181 gcgccagccg cgcgggtcgc gggccgcgag cagggtgtcg ccggcgagca cgaccgcgga
      241 gtaggcgccc tcgactccgc ggagcgcgtc ggcgagcttc tcctccggcg cggccgagcg
      301 cgagcgcgcc atccggtgca cgatcacctc ggagtccatc gtggacgcga agatggatcc
      361 ctggtcctcc agctcgaatc gcagctcgct ggagttggtg aggttgccgt tgtgcgcgag
      421 cgcgatgtgt ccgtcgcgga agcgcgcgag caccggctgc gcgttctcga tcgtggagga
      481 gcccgtggtg ctgtagcgcg tgtggccgat cgcggtcgag ccggtgagcg cggcgatgct
      541 ctccgggctg aacttgtccg acacgagtcc catcgcccgc aggttccgcg cggttccgtc
      601 gtcgccgacc gcgacgattc cggccgattc ctgcccgcgg tgctgcagcg agtacagccc
      661 gaggtgggtc agcgaggccg cgtcgggatg gccgcgaatg ccgaatacgc cgcacatggc
      721 gctagacggt ggtcacggct gcctgctcca gcacggcggc gctctcgacg ctcccctcca
      781 tgatcgccgg gatcgcgtcg tggtatgccg ccgccagccg gtcgagcggg gcaaccagcg
      841 cgcggttgtt gccgtgctcg atctcgagat tccccgagcc ggtgcgcacg cgtccgatca
      901 ccatcggcgg caccatgtgc ttgacagcga tcctcgcgat ctcggcaagt gaagactccg
      961 gcaccgtcac gatgatcctc gcttgcgctt cgttgtacag cagctcccgc agcggtacgt
     1021 ccgcccaggc agagagatct atctccgcgc cgacgacggc cgaccggttg gcgatcgcgc
     1081 actcggccac cgcgaccccg agcccgccgt cgctgcagtc gtgcgccgag cgcacgtggc
     1141 cggcgcgaat gcactcgagc aggcacgcga tcgtgtcgcg ctcgagctcg ggatcgcatt
     1201 tcggcggagc gcccgcgacg gtcctgtgga tccgcgcgag atactcgctc ccaccgaggc
     1261 cgtccgccgg ctcgcccagg atctcgccga gcaggacgat cacgtcgccg tccacggacc
     1321 aggccgcggg cgtcacgtgc gcgatgtcgt caatgagccc gagcatcccg atcaccggcg
     1381 taggatacac tgcgccagag ggactctcgt tgtacagcga cacgttgccg ccggtgacgg
     1441 gggtgtccag cgcgcggcac gcttcgccca tccccgcgat cgcctcgctg agctgaaaaa
     1501 agttctccgg ccgcctgggg ttgccgaagt tcaggcagtt ggtgatagcg agcggacgcg
     1561 cgccggcgca cgccacgttc cgcgctgcct cgcataccgc cgcgcgcgcg ccgttcctcg
     1621 gcgataggta cacgtaccgc ccgttgcagt cggtcttgac cgcgatcgcc ttgttggacc
     1681 cgcgcagccg cagcacggcc gcgtcgccgc cgggcccgat caccgtgttg gtgcgcaccg
     1741 tggagtcgta ttgccggtac gcccacgcct tgctcgcgat cgtcggggaa gagagcatgc
     1801 gctcgagcgt ccagagcggg tccgcttcct cggggagctc cggaatgctc gcgacgtcgg
     1861 tctcgcgcag gcggacgatc gcgtcgctct cgcgcgcttc cggcgagtag cgggggcagt
     1921 cggtcaccag cctcgagccc gggaactcgg caaccacggt gtcgccttcc gtcacccgat
     1981 acacgggctc ggcgatgact tcgccgatca cggccgcggt cagatcccac cgctgcagga
     2041 tctcggcgac ggcgttctcg tggccacgct tcgcgacgac cagcattcgc tcct
//
LOCUS       DASTZR010000184         4415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_924509, whole genome shotgun sequence.
ACCESSION   DASTZR010000184 DASTZR010000000
VERSION     DASTZR010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924509"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="VFV20_04065"
     CDS             <1..893
                     /locus_tag="VFV20_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEU5287555.1"
                     /translation="NKVGLKGPLTTELGTGMRSVNVALRKEFDLYANLRPAKTYQGVP
                     APMGNVDLVVVRENIEDLYAGIEFDIDTPELAKVRAVIEEQAKTKIREDAAIAIKYIS
                     VMGSTRIVKFAFDYARANKRRKVTAVHKSNIMKFADGLFLRVAREVAQGYPDIEFEDR
                     IVDNMCMQLVQKPELYDVLVLPNLYGDILSDLVAGLIGGLGVAPGANIGADVAVFEAI
                     HGSAPKYAGQNKVNPMALMLAGVLMLRHIGEQDAGDKLERAIAKVVADGKDVTYDLRP
                     KGTPVGTKEFADAVIRRVKG"
     gene            896..1297
                     /locus_tag="VFV20_04070"
     CDS             896..1297
                     /locus_tag="VFV20_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005580025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU5287556.1"
                     /translation="MAAPERSRYSTRTKYEFQFGYSRAVRQGDAIRVSGTAGLGEDGM
                     VVSDDVVEQMRRAIEIVQKAIEELGGTLEDVIMTRIYVSDVRNIDSVAVVHGEVFREI
                     QPASSIVRVEFIDPKILVELEAEAICGARKG"
     gene            1304..2287
                     /locus_tag="VFV20_04075"
     CDS             1304..2287
                     /locus_tag="VFV20_04075"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEU5287557.1"
                     /translation="MGKTGRVVVTGATGQVSYALLPHIAAGAMFGPDTEVDLRLSDIP
                     PVIEKAKGVLMELEDGAYPLLANITASADRGVTHRDADWVLLVGSVPRGPGMERGDLI
                     RKNGPIFVEEGKAIAQHAAPTVRVAVVGNPCNTNCLIARANAKQVPDDRWTAMTRLDQ
                     NRARSLLAQRAGARVRDVTQLVIWGNHSATLFPDFTNAKIKGRSASESVDKAWLEAEF
                     IPVVQQRGAAVIAARGASSAFSAAQALVDHVRSLVTPTPASDWFSAAVVSDGSYGVPK
                     GLVSSFPLRSKGDGGYEIVQGVELNDYAKGKLQATIAELEKERDLVKDLLA"
     gene            2284..>4415
                     /gene="acnA"
                     /locus_tag="VFV20_04080"
     CDS             2284..>4415
                     /gene="acnA"
                     /locus_tag="VFV20_04080"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEU5287558.1"
                     /translation="MSYRMDSLTGTDARYYPLTQVGDTTKLPMTVKILLEQMIRAAAT
                     GGASDRDIAALAKYPAPPPEGASVPFRPTRILLQDYTGVPAAVDLAAMRAAMQRAGKD
                     PARIEPLIPVDLIIDHSVQADFFGAKDVYERNLEREYGRNRERYALLRWAGQAFKTFR
                     VVPPGAGICHQVNLERLSRVVQLRDGVAMPDTLFGADSHTTMVNGLGVFGWGVGGIEA
                     EAAMLGQPTYLPWPIVVGVRLTGALPTGTTATDLVLTLVEQMRQHGVVNKFVEFTGDG
                     LGSLTVQDRATVSNMCPEYGATAAFFPVDAQTLRYLKNTGRGDVVPLVERYTKAQGMF
                     RTDGSAIPTFDEMMELDLSSVRPSVAGPKRPQDRHELPMVWQSFLGPKPTKGVTPAGQ
                     TEGPKPVNDAAQTQVASATAATSVVAKRAPTEGAISDGAVVIAAITSCTNTSNPSVMI
                     AAGLLAKKAVERGLTVKRHVKTTMAPGSRVVTRYLDQAGLTPYLEKLGYYLVGYGCTV
                     CIGNSGPLATPEIEQEVKDHDLNVVSVLSGNRNFEGRIHPLVKSSYLASPPLVVAYAL
                     AGTVQIDLSDQPIGDDKDGKPVFLKDIWPTQEEVNLVIERSISEEMYSTEYGKIFEGD
                     SFWKSMPSPTGAMFDWDAKSTYVQEPPFFKELGDARDVADIRDARVLVMLGDSVTTDH
                     ISPAGDIALKSPAGRYLEAHGIKKVDFNS"
BASE COUNT          772 a         1507 c         1487 g          649 t
ORIGIN      
        1 gcaacaaggt gggcctgaag ggtccgctca cgaccgagct cggcaccggg atgcgctcgg
       61 tgaacgtcgc gctgcgcaag gagttcgacc tgtacgcgaa cctccgcccg gccaagacgt
      121 accagggcgt cccggcgccg atgggcaacg tcgacctcgt cgtcgtccgc gagaacatcg
      181 aggacctcta tgcggggatc gagttcgaca tcgacacgcc cgagctcgcg aaggtgcgcg
      241 cggtcatcga ggagcaggcc aagaccaaga tccgcgaaga cgccgccatc gcgatcaagt
      301 acatcagcgt gatgggttcg acccggatcg tgaagttcgc gttcgactac gcccgcgcga
      361 acaagcgacg gaaggtcacc gccgtccaca agtcgaacat catgaagttc gccgatggcc
      421 tcttcctccg tgtcgcgcgt gaggtcgcgc agggctatcc ggacatcgag ttcgaggatc
      481 gcatcgtcga caacatgtgc atgcagctgg tccagaagcc cgagctctac gacgtgctgg
      541 tgctgccgaa cctctacggc gacatcctga gcgacctcgt cgccggcctc atcggcggcc
      601 tcggcgtggc gccgggcgcg aacatcggcg ctgacgtcgc cgtgttcgag gcgatccacg
      661 gatcggcgcc gaagtacgca ggtcagaaca aggtgaaccc gatggcgctg atgctcgccg
      721 gcgtcctcat gctccgtcac atcggcgagc aggacgcggg cgacaagctc gagcgagcca
      781 tcgccaaggt cgtggccgac ggcaaggacg tcacgtacga ccttcggccc aagggaacgc
      841 cggtcggcac gaaggaattc gccgacgccg tgatccggcg cgtgaagggc tagccatggc
      901 ggcgcccgag cgcagtcggt actcgacgcg cacgaagtac gagttccagt tcggctactc
      961 ccgtgcggtg cggcagggcg acgccatccg cgtctcgggc accgcgggtc tgggcgagga
     1021 cggcatggtc gtctccgacg acgtcgtcga gcagatgcgt cgcgcgatcg agatcgtgca
     1081 gaaggccatc gaggagctcg gcgggacgct cgaggacgtg atcatgaccc gcatctacgt
     1141 ctccgacgtg cgcaacatcg atagcgttgc cgtggtccac ggcgaggtct tccgcgagat
     1201 ccagccggct tcctcgatcg tccgggtcga gttcatcgac ccgaagatcc tggtcgagct
     1261 cgaggccgag gcgatctgcg gggctcgaaa ggggtaggtc cagatgggta agaccggtcg
     1321 cgtggtcgtc accggtgcaa caggccaggt ctcgtacgcg ctcctcccgc acatcgccgc
     1381 gggcgcgatg ttcggtccgg acaccgaggt cgacctacgc ctgtccgata tcccgccggt
     1441 catcgagaag gccaagggcg tcctcatgga gctcgaggac ggcgcctacc cgctcctcgc
     1501 gaacatcacc gccagcgcgg accgcggcgt cacccatcgc gacgccgact gggtcctcct
     1561 cgtgggcagc gtgccgcgcg gcccgggcat ggagcgcggc gacctcatcc ggaagaacgg
     1621 accgatcttc gtcgaggagg gcaaggcgat cgcgcaacac gcggcgccga ccgtgcgcgt
     1681 cgccgtggtc gggaatccct gcaatacgaa ctgcctcatc gcgcgcgcga acgccaagca
     1741 ggtccctgac gaccgctgga cggcgatgac gcggctcgac cagaaccgcg cacgctcgct
     1801 cctggcgcag agggccggcg cgcgcgtgcg cgacgtgaca cagctcgtca tctggggcaa
     1861 tcacagtgcg acgctcttcc ccgatttcac gaacgcgaag atcaaggggc ggtccgcgtc
     1921 ggagtcggtc gacaaggcgt ggctcgaggc ggagttcatc ccggtcgtcc agcagcgcgg
     1981 cgccgcggtc atcgcggcgc gcggcgcgtc gagcgcgttc tcggccgcgc aggccctcgt
     2041 cgaccacgtg cgcagcctcg tcacgccgac cccggcgagc gactggttca gcgccgcggt
     2101 cgtgagcgac ggctcgtacg gcgtgccgaa gggcctcgtc tcgtcgttcc cgctgcgctc
     2161 gaagggggac ggcggctacg agatcgtgca gggcgtggag ctcaacgact acgcgaaggg
     2221 caagcttcag gcgaccatcg ccgagctcga gaaggagcgc gacctggtga aggaccttct
     2281 cgcatgagct accggatgga ctcgctcacc ggcacggacg cgcgttacta cccgctgacg
     2341 caggtgggcg acacgacgaa gctcccgatg accgtgaaga tcctgctcga gcagatgatc
     2401 cgcgcggccg ccaccggcgg cgccagcgat cgcgacatcg cggccctcgc gaagtacccg
     2461 gcgccaccgc ctgagggcgc cagcgtgccg ttccggccga cccgcatcct gctccaggac
     2521 tacaccggcg tccccgcggc ggtcgatctc gccgcgatgc gcgccgcgat gcagcgcgcg
     2581 ggcaaggatc cggcacgcat cgagccgctc atcccggtgg acctcatcat cgaccactcg
     2641 gtccaggccg acttcttcgg cgcgaaggac gtgtacgagc ggaacctcga gcgcgagtac
     2701 ggtcgcaacc gcgagcgcta cgcgctcctg cgctgggccg ggcaggcgtt caagacgttc
     2761 cgcgtcgtcc cgccgggcgc cggcatctgc caccaggtga acctcgagcg cctctcgcgc
     2821 gtggtgcagc tgcgcgacgg cgtggcgatg cccgacacgc tcttcggcgc cgactcccac
     2881 accacgatgg tgaatgggct cggcgtgttc ggctggggcg tcggtggcat cgaggccgag
     2941 gccgcgatgc tcggccagcc cacctatctg ccatggccga tcgtcgtggg cgtgcggctc
     3001 acgggtgcgc tgccgacggg cacgacagcg accgatctcg tcctcacgct cgtcgagcag
     3061 atgcgccaac acggtgtggt gaacaagttc gtcgagttca ccggcgatgg cctcggctcg
     3121 ctcacggtgc aagaccgcgc cacggtctcg aacatgtgcc cggagtacgg cgcgaccgcg
     3181 gcgttcttcc ccgtcgacgc gcagaccctg cgctatctca agaacaccgg tcgtggtgac
     3241 gtcgttccgc ttgtcgagcg gtacaccaag gcgcagggga tgttccgcac cgacggatcg
     3301 gcgataccca cgttcgacga gatgatggag ctcgacctct ccagcgtgcg accgagcgtc
     3361 gcaggtccga agcggccgca ggaccgtcac gagctgccga tggtctggca gtcgttcctc
     3421 ggtcccaagc cgacgaaggg cgtcacgcca gccgggcaga ccgaaggccc aaagccggtc
     3481 aacgacgcgg cgcagacgca ggtcgcgtcc gcgaccgcgg cgacctcggt cgtcgcgaag
     3541 cgagcgccca ccgaaggcgc gatcagcgac ggcgcggtgg tcatcgccgc catcacctcg
     3601 tgcacgaaca cgtcgaatcc gtcggtgatg atcgcggccg gtctcctcgc gaagaaggcc
     3661 gtcgagcgcg ggctcaccgt gaagcggcac gtgaagacga cgatggcgcc gggctcgcgg
     3721 gtcgtcacgc gctatctcga ccaggccggc ctcacgccat atctcgagaa gctcggctac
     3781 tacctcgtcg gctacggatg cacggtctgc atcgggaact ccggtccact tgcgacgccg
     3841 gagatcgagc aagaggtgaa ggaccacgac ctcaacgtcg tttccgtcct ctcgggcaac
     3901 cgcaacttcg aggggcgcat ccatccactg gtgaagtcga gctacctcgc gtcgccgccg
     3961 ctcgtggtcg cgtacgccct ggcgggcacc gtccagatcg acctgtccga tcagccgatc
     4021 ggcgacgaca aggacggcaa gccggtcttc ctgaaggaca tctggccgac gcaagaagaa
     4081 gtgaacctgg tgatcgagcg ctccatcagc gaggagatgt actcgacgga gtacgggaag
     4141 atcttcgagg gcgacagctt ctggaagtcg atgccctcgc cgacaggagc gatgttcgac
     4201 tgggacgcga agtccacgta cgtgcaggag ccgcccttct tcaaggagct cggcgatgcg
     4261 cgcgacgtcg ccgacatccg cgacgcccgc gtgctcgtga tgctcggcga ctcggtgacg
     4321 accgaccaca tctcgcccgc cggcgacatc gccctcaaga gcccggccgg ccgctacctc
     4381 gaggcgcacg gcatcaagaa ggtcgacttc aactc
//
LOCUS       DASTZR010000185         3056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_872289, whole genome shotgun sequence.
ACCESSION   DASTZR010000185 DASTZR010000000
VERSION     DASTZR010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872289"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="VFV20_04085"
     CDS             <1..564
                     /locus_tag="VFV20_04085"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5287559.1"
                     /translation="TLLASEGVGEAAGQAHRLRFGLRAPDLERELERAGQAALAGDAA
                     TLDEAVSVFRRALEVEPGLWEAHFGLGIVARHRSDAEGAHAEFSKVLELSPDQPDALH
                     ELGVTLLMRDDTNAAVRALERAASLRPDDPAYAADAGFAHLRAGNLESARRHLERASG
                     IDANDPITKSYVGELLRVESEVGKHRQ"
     gene            566..1828
                     /locus_tag="VFV20_04090"
     CDS             566..1828
                     /locus_tag="VFV20_04090"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5287560.1"
                     /translation="MLGSVAMKRVVIVGGGFAGLHLVRRLEGMLTPDEVEVTVIDRNN
                     FHLFTPLLYQVATGELPPHAVAYPLRVPLARAGYNFVRAEVTAIDLAQHLVRTGEGAY
                     PYDHVLIVPGSVTNDFGIPGVREHAVTLKWLEDGRRVRHRILSVFEEASREPDPEKRG
                     RLLSFVIVGAGPVGIELAASMRDLMDHTLRRIYPRIDFDRDPRVTIVDASERVLPQMD
                     ERLSRIAARRMTELRVNVLPRTVVGEVGPHSIRTTSGIELAAQTVIWAGGVRANPLLG
                     ALDLPKWKDGRLLVDEQFRADGRDDVLSFGDAAAFLEHGKPLPQLAQVAVLQAPAAAR
                     NIAHLVRGEPLEAYRYHRKGDLIALGRTKAGAEFARPVRIVVSGFLAWTVWRVNYLMQ
                     LVGVRNRGTLLIEWILSYFSRRIVTDIP"
     gene            1839..2765
                     /locus_tag="VFV20_04095"
     CDS             1839..2765
                     /locus_tag="VFV20_04095"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEU5287561.1"
                     /translation="MPLRIDASEARDFLRARGHDASDLEPLSGGEWSTAFAFRTGDRE
                     YVVRFHERRDDLEKDRYAQRWVSGRLRTPRIVEIGDLAGGAYGISERVRGPAIDTLDE
                     AGMRRALPSLLAAMDAMREADLAGTRGYWGWHGDGNAEAPSWRDTLLGRLPGRDVMGE
                     WRAAVVASPLGITAFDAAVAGIHELLPFCPEERALVHEDLLNYNVLVDDRGVVLLDWG
                     ASWYGDFVYDVALLTFWWPRYERWPRIAIREEVERHWREIGLRVPSFAERLRCCQLHI
                     GIEHMPYQAARGRLDDARWTASHTLELLDAPL"
     gene            complement(2795..>3056)
                     /gene="fusA"
                     /locus_tag="VFV20_04100"
     CDS             complement(2795..>3056)
                     /gene="fusA"
                     /locus_tag="VFV20_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908219.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU5287562.1"
                     /translation="GDVLADLNGRRGRVLGMESTGAGYQSIKAHVPLAEIFRYATDLR
                     SMTGGRGSFSSRFLSYEECPSHIAQKVIAAHEEQAEETAAAH"
BASE COUNT          413 a         1092 c         1091 g          460 t
ORIGIN      
        1 accctcctcg cttcagaggg cgtgggtgag gcggcgggcc aggcgcatcg attgcgcttc
       61 ggtctgcgcg caccggatct cgaacgcgag ctcgagcggg cgggccaggc cgccctcgcg
      121 ggagacgccg cgacgctcga cgaggccgtg tcggtcttcc gtcgggcgct cgaggtggag
      181 cccggtctct gggaggcgca cttcggcctc ggcatcgtcg cgcgtcaccg gtccgacgcg
      241 gagggcgcgc acgcggagtt ctccaaggtc ctcgagctgt cgccggacca gcccgatgcg
      301 ctccacgagc tcggcgtgac gctcctgatg cgcgacgaca cgaatgcggc ggtacgcgcg
      361 ctcgagcgcg ccgcgtcgct gcggccggac gatcccgcct acgccgccga cgccggattc
      421 gcgcatctgc gcgctggcaa cctcgagagc gcgcgccgcc atctcgagcg cgcctccggc
      481 atcgacgcga acgacccgat cacaaagtcg tacgtcggag agctgctgcg ggtcgaatcc
      541 gaggtaggga agcaccgcca gtgacgtgct cggatccgtc gcgatgaagc gcgtcgtcat
      601 cgtcggcggc ggcttcgcgg ggctgcatct cgtccgccgc ctcgagggca tgctcacgcc
      661 ggacgaggtc gaggtcacgg tcatcgatcg caacaacttc catctcttca caccgctcct
      721 ctaccaggtc gcgaccggcg agctgccgcc gcacgccgtc gcgtatccgc tgcgcgtgcc
      781 gctcgcccgc gcggggtaca acttcgtgcg ggcggaggtc accgcgatcg acctcgcgca
      841 gcacctcgtg cggaccggcg agggtgccta cccgtacgac cacgtgctga tcgtcccggg
      901 gtcggtgacg aacgacttcg gcataccggg agtgcgcgag cacgccgtga cgctgaagtg
      961 gctcgaggac gggcggcgcg tgcgccaccg gatcctctcg gtgttcgagg aggcgtcgcg
     1021 cgagccggat cccgagaagc gcggccgcct gctctcgttc gtcatcgtcg gcgccggacc
     1081 cgtcggcatc gagctcgccg cgtcgatgcg tgacctgatg gatcacacgc tgcgccggat
     1141 ctacccccgt atcgacttcg atcgcgaccc gcgcgtcact atcgtcgacg cgtccgagcg
     1201 cgtgctgccc cagatggacg agcgcctctc ccggatcgcc gcgaggcgta tgaccgagct
     1261 gcgcgtgaac gtgctgccgc ggacggtcgt gggcgaggtc ggtccgcact ccatccgcac
     1321 gacgtcaggg atcgagctcg cggcccaaac ggtgatctgg gcgggcggcg tgcgtgcgaa
     1381 tccgctcctc ggggcgctcg atctgccgaa atggaaggac ggccggctcc tcgtcgacga
     1441 gcagttccgc gccgatggcc gcgacgacgt gctctcgttc ggcgacgccg cggcgttcct
     1501 cgagcacggc aagccgctgc cgcagctcgc gcaggtggcc gtgctccagg cgcccgcggc
     1561 cgcacggaac atcgcgcacc tcgtccgcgg cgagccgctc gaggcgtacc gctaccaccg
     1621 caagggcgat ctcatcgcgc tcggccggac caaggcgggc gccgagttcg cgcggccggt
     1681 gcgcatcgtc gtgtcggggt tcctcgcgtg gaccgtctgg cgggtgaact acctcatgca
     1741 gctcgtcggc gtgcgcaacc gcggcacgct gctcatcgaa tggatcctga gctacttttc
     1801 tcgccggatc gtgaccgaca tcccctagat tccgcggcat gcctctccgg atcgacgcgt
     1861 ccgaggcccg cgacttcctg cgcgcacgcg gccacgacgc gagcgacctc gaaccgctgt
     1921 ccggtggcga gtggtcgacc gcgttcgcct tccgcacggg cgaccgcgag tacgtggtcc
     1981 gcttccacga gcgtcgcgac gatctcgaga aggaccgcta cgcgcagcgc tgggtctccg
     2041 ggcggctacg cacgccgcgg atcgtcgaga tcggcgacct cgccgggggc gcgtacggga
     2101 tctccgagcg ggtgcggggc cccgccatcg acacgctcga cgaggcgggg atgcgccgcg
     2161 cgctgccgtc gctgctcgcc gcgatggacg cgatgcgcga ggcggacctc gccgggacac
     2221 gcggctactg gggctggcac ggcgacggga acgccgaggc cccgagctgg cgcgacacgc
     2281 tgctcggacg gctgcccggg cgcgacgtga tgggcgagtg gcgggcggcc gtcgtggcgt
     2341 cgccgctggg gatcacggcg ttcgacgccg ccgtcgcggg cattcacgag ctgcttccgt
     2401 tctgccctga ggagcgcgca ctcgtgcacg aggacctcct gaactacaac gtcctcgtcg
     2461 acgaccgcgg tgtcgtgctc ctcgactggg gcgcgtcctg gtacggcgac ttcgtctacg
     2521 acgtcgcgct gctgacgttc tggtggccgc gctacgagcg gtggccacgc atcgccatcc
     2581 gcgaggaggt cgagcggcac tggcgcgaga tcggtctgcg cgttccgtcg ttcgcggagc
     2641 ggctccgctg ctgccagctg cacatcggga tcgagcacat gccctaccag gccgcgcgcg
     2701 ggcggctcga cgacgcgcgc tggacggcga gccacacgct cgagctcctc gacgcgccgc
     2761 tctgacacgg caggaggcgg ccgacggcga acggctaatg cgccgccgcg gtctcctcag
     2821 cctgctcctc gtgcgcggcg atgaccttct gcgcgatgtg cgacgggcat tcttcgtagc
     2881 tcaggaaccg tgagctgaag ctgccgcgcc cgcccgtcat cgaccggagg tcggtcgcgt
     2941 agcggaagat ctcggcgagc gggacgtgcg ccttgatgct ctggtagccg gcgcccgtgg
     3001 actccatgcc gaggacgcga ccgcgccggc cgttcagatc ggcgaggacg tcgccc
//
LOCUS       DASTZR010000186         2113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_454455, whole genome shotgun sequence.
ACCESSION   DASTZR010000186 DASTZR010000000
VERSION     DASTZR010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454455"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..173)
                     /locus_tag="VFV20_04105"
                     /pseudo
     CDS             complement(1..173)
                     /locus_tag="VFV20_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894141.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
     gene            complement(326..835)
                     /locus_tag="VFV20_04110"
     CDS             complement(326..835)
                     /locus_tag="VFV20_04110"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEU5287563.1"
                     /translation="MTLADGIAIARALALAPVAWAILADQNVLALAIFAVAAASDAVD
                     GRLARRAGTTGTLGAYLDPIADKILVVGTLLALAGAGRGWPVTVVTILAIVREGIAAI
                     LRTRALARGAAQPADRVAKLKTAAEMIGVALIILDGRPWAVLGTGIAGIALLVGLATL
                     PRYATTRAA"
     gene            complement(832..2001)
                     /locus_tag="VFV20_04115"
     CDS             complement(832..2001)
                     /locus_tag="VFV20_04115"
                     /inference="COORDINATES: protein motif:HMM:NF020700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEU5287564.1"
                     /translation="MARLGELLRAQREKKGITLDQAAADTRIREKFLKALEDADYQSL
                     PGAVYTKGFLRNYAEYLDLDQEELVVLFHEERGGAPAPESSRAFRPMRPIMRRSLIFT
                     PAVLVPIIVLAGIGLFVGYLYYQFTSFAVAPALEVLDPASDAIAQNAQFVLRGRTVPT
                     GKVTIQVFPGPETIADIHPDADGAFSASIDLTPGANHVVIEVLDQTGKVSRVSRSIML
                     QPQATASAAPVKLVVESPANGARIENSAVTVSGTTDGTLTINGAPVPVVAQAGRFEAR
                     ITFPAGAQTITIVSRNPAGASVTETRTVTVAYTAAVIQIALKGGDAWLEVVVDGAKVP
                     GTGHIYKDGETATFTGKQVTVRTGNAAATQVTHNGVFEGALGTQGQVVEKVYSAQ"
BASE COUNT          290 a          721 c          792 g          310 t
ORIGIN      
        1 gacgagaccg cgccgtgcga ggcgatcagc tggggcgcga cgtccgcgag gatcgtcttc
       61 gcgtcgtcgc tgtacacgat cgacccgccg cgcaggtacg cgctcgaccc gccgcgcgcc
      121 gagaggacgg tggcgaggag ccccccggtg caggactcgg cgacggcgat ggtgatcttg
      181 cgcttgacca accgcgggcc gatcacgccc atgcgctcga ccagctgatc gtacgtgggg
      241 ttcgtcttcg ccatgcgagg aggttagcgc cgcgtgcgcg aggccgggac gcgagcgggt
      301 gcgcgcggtg aagactgcac gacgatcagg cggcgcgcgt cgtcgcgtac cgcggcagcg
      361 tcgcgagtcc gacgaggagc gcgataccgg cgatgccggt cccgaggacc gcccacggcc
      421 ggccgtccag gatgatgagc gcgacgccga tcatctcggc ggcggtcttg agcttcgcga
      481 cgcggtccgc gggctgcgcg gcgccgcgcg cgagggcccg cgtgcggagg atcgcggcga
      541 tcccctcgcg gacgatcgcg aggatggtga cgaccgtgac cggccatccg cggcccgcgc
      601 ccgcgagggc gaggagcgtg ccgacgacga ggatcttgtc ggcgatcggg tcgaggtacg
      661 cgccgagcgt gcccgtggtg ccggcgcggc gcgcgagtcg cccatcgacc gcgtcagacg
      721 ccgccgcaac ggcaaagatc gcgagcgcga ggacattctg atccgcgagg atggcccacg
      781 cgaccggggc gagcgcgagt gcgcgtgcga tcgcgatgcc gtcggcgagg gtcactgcgc
      841 gctgtacacc ttctcgacga cctggccctg cgttcccagc gcaccctcga agacgccgtt
      901 gtgcgtgacc tgggtcgccg cggcgttccc cgtccggacc gtcacctgct tgcccgtgaa
      961 cgtcgcggtc tcgccgtcct tgtagatgtg gccggtgccc ggcaccttcg cgccgtcgac
     1021 gacgacctcg agccacgcgt cgccgccctt cagcgcgatc tggatgaccg cggccgtgta
     1081 ggcgacggtg acggtgcgcg tctcggtcac gctcgcgccg gccggattgc gcgacacgat
     1141 ggtgatggtc tgcgcgcccg cggggaaggt gatccgcgcc tcgaaccgcc ccgcctgcgc
     1201 gacgaccggc acgggcgcgc cgttgatcgt cagcgtcccg tcggtggtcc cgctcacggt
     1261 gaccgcgctg ttctcgatgc gcgcgccgtt cgcgggcgac tcgacgacga gcttcaccgg
     1321 tgccgcgctc gcggtcgcct gcggctgcag catgatgctg cgcgacacgc ggctgacctt
     1381 gccggtctgg tcgaggacct cgatgacgac gtggttcgca cccggcgtga ggtcgatcga
     1441 cgcggagaag gccccgtcgg cgtcggggtg gatgtccgcg atggtctcgg gtccggggaa
     1501 cacctggatc gtcaccttgc cggtcgggac ggtccgtcct ctgagcacga actgcgcgtt
     1561 ctgcgcgatc gcgtcgctgg cgggatccag cacttcgagc gcgggcgcga cggcgaacga
     1621 ggtgaactgg taatagagat agccgacgaa gagcccgatg ccggcgagga cgatgatggg
     1681 cacgaggacg gcgggcgtga agatgaggct ccgccgcatg atcgggcgca tcggccggaa
     1741 tgcgcgcgac gactcagggg cgggcgcgcc gccgcgctcc tcgtggaaga gcacgaccag
     1801 ctcctcctgg tcgaggtcga ggtactcggc gtagttgcgg aggaagccct tcgtgtacac
     1861 ggcgccgggg agcgactggt agtcggcgtc ctccagcgcc ttgaggaact tctcgcggat
     1921 gcgcgtgtcc gcggcggcct ggtcgagggt gatgcccttc ttctcacgct gggcgcgaag
     1981 cagctcgccg aggcgtgcca cggctattcc tgggcgtccg gcgggacccc gtacacctcg
     2041 cggaacttgc cggggccacg atcgggcccc accacgccgc gctgctccat catgtcgatg
     2101 agccgcccag cgc
//
LOCUS       DASTZR010000187         3772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_973573, whole genome shotgun sequence.
ACCESSION   DASTZR010000187 DASTZR010000000
VERSION     DASTZR010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973573"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VFV20_04120"
     CDS             complement(<1..377)
                     /locus_tag="VFV20_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287565.1"
                     /translation="MRRLMVGAAALIAALLATTTPAAACAGLIGPNGAVNLLRTSTFA
                     GYHDGVEHYVTAFQFAGGSGQFGSLVPLPGIPTDVIRGGDWTLQRLVRETTPVEKFGV
                     FRSSLAQSAAADAVVLQQVRIDAL"
     gene            complement(467..1285)
                     /locus_tag="VFV20_04125"
     CDS             complement(467..1285)
                     /locus_tag="VFV20_04125"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol ring-cleavage dioxygenase"
                     /protein_id="HEU5287566.1"
                     /translation="MALVFASIAPHGSMAIAEWCTPDQRPLAARTRAAFEELGRRFDA
                     ARPAVTVLLTPHHVHVDGHMAVVTAGAMHGVLEGGAAPVELRARVDRDLTDAVRAAIA
                     AAGVPIVGLSYGANDAAAAVMPMDWATQVPIHFMGRDAVPIVMVAPARDLSWEMHVAA
                     GRAIARAAGASGKRVALIASCDHGHGHDANGPYGYSPASKVFDDRVVGLVERSALGEL
                     LTFAPEFVADAKADSFWQMLMLHGAIGDRWTAELLSYEAPTYFGMLCAAFTPPD"
     gene            complement(1285..1791)
                     /locus_tag="VFV20_04130"
     CDS             complement(1285..1791)
                     /locus_tag="VFV20_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287567.1"
                     /translation="MDDLFRILVQFLHVFTGVLWIGGGLYTLFVQTPSLMAAPPQARG
                     PVLGQLIPRQVTYLLRLGEVTIATGILNLFASGRGRELENVLGSRWAVAIVIGALLAI
                     VLLGLGHAVIKPSAMRLLQVGARVAAGDAAAPAEAQAIIARLRTVGYAQIVLGLVIVL
                     AMVTARFS"
     gene            1811..2911
                     /gene="dnaJ"
                     /locus_tag="VFV20_04135"
     CDS             1811..2911
                     /gene="dnaJ"
                     /locus_tag="VFV20_04135"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU5287568.1"
                     /translation="MATATEYYALLGVPRNATEEQIRSAYRKLARQYHPDVSKDDGAS
                     EKFKQITEAYEVLSDPERRQRYDMFGAGQGGLGDFGIGDLFETFFGTEFRRRESRGPM
                     RGADLRMEIEIELLDAVKGGERTIRVPRLETCGKCDGSGAEPGSEISTCSTCGGRGEV
                     RQVQQSVFGRFVNVATCPRCGGAGRTVDRLCTRCRGEGRERREHDLGLTIPPGIDDGQ
                     QLRVAGEGEAGMRAGPSGDLYVLIRIKEHPLFRREGDDLVHVLRISPAQAALGDELSV
                     PTIDEAEASVRVPAGAQHGQVVRLRGKGVPHLGSSGRGDQLVYLDVAVPRTLTKDQRA
                     LYKRLRELEGTPEQMDDARGVFDRIRDAIGGE"
     gene            2908..>3772
                     /gene="prmA"
                     /locus_tag="VFV20_04140"
     CDS             2908..>3772
                     /gene="prmA"
                     /locus_tag="VFV20_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEU5287569.1"
                     /translation="MTPPRRWYELAVPAHAEAVEAVSEILTRVGHRGIAVDLPVEPRG
                     GTDHTVKAYLLVDGEVVARVNDVRDALGHLQAFGLGPIGELAVREIAEEDWLESWKAQ
                     FTPLRIGRFLVRPTWSDAPVPADAVELVLDPGMAFGTGLHPTTQQCLEAVSALHLEAR
                     SVLDVGTGSGILAIAAAKRGASPVVAVDTDPLSVDAARENAERNRVALPVGLGSAADV
                     PGRFEIVIANIVAPVLQRIAPDLAARLAPKGTLVVAGISAPNERDTRDALARAGLRTT
                     IDRNQRDDWVAL"
BASE COUNT          563 a         1348 c         1381 g          480 t
ORIGIN      
        1 agcgcgtcga tgcgcacctg ctggagcacc accgcgtcgg ccgcggccga ctgcgcgagg
       61 gagctgcgga acacgccgaa cttctccacc ggtgtcgtct cgcgcacgag gcgctggagc
      121 gtccagtcgc cgccgcggat cacgtccgtg gggatgccgg gcagtggaac gagcgagccg
      181 aactgcccgc tccctcccgc gaactggaac gccgtgacgt agtgctcgac gccgtcgtga
      241 tagccggcga acgtcgaggt gcggagcagg ttgaccgcgc cgttggggcc gatgagtccc
      301 gcgcaggccg cggccggtgt ggtggtcgcc aggagcgcgg cgatcagtgc cgcggccccg
      361 accatgagcc gtcgcatacg tcgtccctcc atccggcaga cccggatgga ggacagacga
      421 cggcgggggc gggccggttc cctgatgacg gccgacgccg ctcggctcag tcgggcggcg
      481 tgaaggccgc gcagagcatc ccgaagtacg tgggtgcctc gtacgacagg agctccgcgg
      541 tccaccggtc gccgatcgcg ccgtggagca tcagcatctg ccagaacgaa tcggccttcg
      601 cgtccgcgac gaactccggc gcgaacgtca gcagctcgcc gagcgcgctc cgctccacga
      661 gccccacgac cctgtcgtcg aagaccttcg acgccggcga gtacccgtag ggcccgttcg
      721 catcatgacc gtggccgtga tcgcaggagg cgatcagggc gacgcgcttc cccgatgccc
      781 cggccgccct cgcgatcgcg cggcccgcgg cgacatgcat ctcccacgag aggtcgcgcg
      841 cgggcgcgac catcacgatc ggcacggcat cacgtcccat gaagtggatc ggcacctgcg
      901 tcgcccagtc catcggcatg acggccgcgg ccgcgtcgtt cgcgccgtac gacagcccca
      961 cgatgggcac ccccgcggcc gcgatggccg ctcgcaccgc gtccgtgaga tcccggtcga
     1021 cccgcgcacg gagctcgacc ggcgcggcgc cgccctcgag gacgccgtgc atcgcgccgg
     1081 cggtgacgac cgccatgtgc ccgtcgacgt ggacgtggtg cggagtgagc aggacggtga
     1141 ccgcggggcg cgccgcatcg aagcggcgcc cgagctcctc gaacgcggcg cgcgtccgcg
     1201 ccgcaagcgg ccgctggtcg ggcgtgcacc actcggcgat cgccatgctg ccgtgcggcg
     1261 cgatcgacgc gaagaccagc gccatcagct gaagcgggcg gtcaccatcg cgaggacgat
     1321 cacgaggccg agaacgatct gcgcgtaacc gacggtccgc aggcgggcga tgatcgcctg
     1381 cgcctccgca ggcgccgcgg cgtccccagc cgcgacgcgc gcaccgacct gcaggagccg
     1441 catcgccgac ggcttgatga ccgcgtgccc gagcccaagg aggacgatcg ccaggagggc
     1501 gccgatgacg atcgcgacgg cccagcgcga gccgagcacg ttctcgagct cccggccgcg
     1561 gccgctcgcg aagaggttga ggatgccggt cgcgatggtg acctcgccca gtcgcaggag
     1621 gtaggtcacc tgccgcggaa tgagctgacc gaggaccggt ccgcgcgcct ggggtggggc
     1681 cgccatgagt gagggggtct gcacgaacag cgtgtacagg ccgccgccga tccagaggac
     1741 tccggtgaag acatgcagga attgcacgag gatgcggaag aggtcgtcca tgaccccgga
     1801 taatgaggca atggcgaccg cgacggagta ctacgccctg ctgggcgtcc ccaggaacgc
     1861 gacggaggag cagatccgct ccgcctatcg gaagctcgcc cgccagtacc accccgacgt
     1921 cagcaaggac gatggggcct ccgagaagtt caagcagatc accgaggcgt acgaggtcct
     1981 gtccgacccg gagcgccgtc agcgctacga catgttcggc gcgggccagg gcggcctggg
     2041 cgatttcggc atcggggacc tgttcgagac cttcttcggc accgagttcc gccgccgcga
     2101 gtcgcggggg cccatgcgcg gcgcggacct ccggatggag atcgagatcg agctcctcga
     2161 cgcggtgaag ggcggcgagc gcaccatccg cgtcccgcgc ctcgagacct gcgggaagtg
     2221 cgacggcagc ggcgccgagc cggggtcgga gatctccacg tgctcgacgt gcggcggccg
     2281 cggcgaggtg cgccaggtgc agcagtccgt cttcggcagg ttcgtgaacg tcgccacctg
     2341 cccgcgctgc ggcggcgcgg gacggaccgt cgaccggctc tgcacgcgct gccgcggcga
     2401 gggccgggag cgtagggagc acgacctcgg gctcaccatc ccgccgggga tcgacgacgg
     2461 ccagcagctg cgtgtcgccg gcgagggcga ggccgggatg cgcgcaggtc cgtcgggcga
     2521 cctgtacgtg ctcatccgca tcaaggagca cccgctcttc cggcgcgagg gcgacgacct
     2581 cgtgcacgtc ctccgcatct cgccggcgca ggcggcgctc ggcgacgagc tctccgtgcc
     2641 gacgatcgac gaggccgagg cgagcgtgcg cgtgcccgcc ggcgcacagc acggtcaggt
     2701 cgtacggctt cgcggcaagg gcgtgccgca tctcggatcg agcgggcgcg gcgaccagct
     2761 cgtgtatctc gacgtcgccg tgccgcggac gctcacgaag gaccagcgcg ccctgtacaa
     2821 gcgcctgcgc gagctcgagg gcacgccgga gcagatggac gacgctcgcg gcgtgttcga
     2881 ccggatcagg gacgccatcg gcggcgagtg acgccaccac ggcgctggta cgagctcgcg
     2941 gtccccgcgc acgccgaagc ggtcgaggcg gtctccgaga tcctcacgcg cgtcggccac
     3001 cggggcatcg cggtcgatct cccggtggag ccgcgcggcg gcaccgatca caccgtgaag
     3061 gcgtacctgc tcgtcgacgg tgaggtcgtc gcgcgggtga acgacgtgcg cgatgccctc
     3121 ggccatctcc aggcgttcgg cctcggcccc atcggcgagc tcgcggtgcg cgagatcgcc
     3181 gaggaggact ggctcgagtc gtggaaggcg cagttcacgc cgctgcgcat cggccgcttc
     3241 ctcgtgcgtc cgacgtggtc ggacgcaccg gtccccgcgg acgcggtcga gctggtcctc
     3301 gatccgggca tggccttcgg caccggtctg catccgacga cgcagcagtg cctggaggcg
     3361 gtgtcggcgc tccacctcga ggcgcggtcg gtgctggatg tcggcacggg ctcgggcatc
     3421 ctcgcgatcg ccgcggcgaa gcggggcgcc tcgcccgtcg tcgcggtcga caccgatccg
     3481 ctctccgtcg atgccgcgcg ggagaacgcc gagcgcaacc gcgtggccct cccggtgggc
     3541 ctcggcagcg ccgcggacgt gcccggccgt ttcgagatcg tgatcgcgaa tatcgtcgcc
     3601 ccagtgctcc agcggatcgc gcccgacctc gcggcccgtc tcgcgccaaa ggggacgctc
     3661 gtcgtcgccg gcatcagcgc gccgaacgag cgtgacacgc gcgacgcgct cgcgcgcgcg
     3721 ggcctgcgca cgaccatcga tcggaaccag cgcgacgact gggtcgcgct cg
//
LOCUS       DASTZR010000188         5908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_742149, whole genome shotgun sequence.
ACCESSION   DASTZR010000188 DASTZR010000000
VERSION     DASTZR010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5908
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742149"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VFV20_04145"
     CDS             complement(<1..516)
                     /locus_tag="VFV20_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEU5287570.1"
                     /translation="MSEKPLWSGRFEGSLDPKIRAFTASLDLDRRIALHDVRGSIAHA
                     RMLGRQGIVTADESAMLVRELERIAAELRDGTFPWPADAEDVHSAVERVLTDRAGGVG
                     GKLHTARSRNDQVALDLRLLVIELLDELDHAVKSLALALVERAEQEIDTVLPGYTHLQ
                     RAQPVSLAHHLL"
     gene            complement(513..1844)
                     /locus_tag="VFV20_04150"
     CDS             complement(513..1844)
                     /locus_tag="VFV20_04150"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEU5287571.1"
                     /translation="MAKSLKGETCVLAYSGGLDTSVAVAWLRETYGLEVVTCTGDLGS
                     VRDLDLVQKKAIASGARKAIVQDARDIFVEYFVWPGLQADALYQDRYPLATALGRPLL
                     AKLLVDAARSEGAKYVAHGCTGKGNDQVRFDLAVGALAPDLKVIAPMRGGMNMTRDEE
                     IDYARKRGIPVEATKKSPYSVDENLWGRSIEAGVLEDPWGAPPRDVFQWTVDPDEAPA
                     KPRELVIEFSEGVPVKLDGEQLGGVALIERLNKLGGRHGVGRIDQIEDRLVGIKSREI
                     YEAPAAVILHAAHKALEEMVLTKEALRFKSAVAREYAELIYNGLWFTAHHQDLAAYVR
                     STQRFVTGEIRVRLDRGHLTIVGRRSPHSLYSTALATYGKGDLFDHRASEGFITVFGM
                     SLRTQARTQALWGEGTAAMLDVPRKELSGPRGGRGGAPAKSSPKRIGAKRR"
     gene            complement(1848..2330)
                     /locus_tag="VFV20_04155"
     CDS             complement(1848..2330)
                     /locus_tag="VFV20_04155"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="regulates arginine biosynthesis when complexed with
                     arginine by binding at site that overlap the promotors of
                     the arginine biosynthesis genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU5287572.1"
                     /translation="MKELARKHKRQQALLRLVQQHRLSTQGDLVRALENAGFVATQAT
                     VSRDIVELGLVKVSRDGTHAYAAPTAVASGGGTDRLRKFCEDYLVEGRLAGNIVVLRS
                     PPGTANAMAIALDTSDFAEIIGTVAGDDTVFVATSTERHARSLLTRLTAFGIARKERS
                     "
     gene            complement(2327..3103)
                     /gene="argB"
                     /locus_tag="VFV20_04160"
     CDS             complement(2327..3103)
                     /gene="argB"
                     /locus_tag="VFV20_04160"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEU5287573.1"
                     /translation="MSDLIVVKIGGSVAGEDAAALDVIAALEDAGHALVVVHGGGPLV
                     SEWATSLGQQTRFVRGLRVTDEPMRDVALAVLAGLANARIVAALTARAVPAVGLSGID
                     GGILRAIREDPELGLVGRITKVDSALLEELVDAGRVPVVAPAAFEERAEGEVEVLNVN
                     ADSAAGAIAASLGARLLVFITDVPGVRGKDGKVIPTLDRDRAKALVDDGTIEGGMLPK
                     VEACLIAAGAGCRAAIVDARSADAIERLLAGERAGTVFTA"
     gene            complement(3207..4406)
                     /gene="argJ"
                     /locus_tag="VFV20_04165"
     CDS             complement(3207..4406)
                     /gene="argJ"
                     /locus_tag="VFV20_04165"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEU5287574.1"
                     /translation="MKEDRHSDSVPRGFTAGAAKAGVKTGVADRLDVGLIVSDRPCVA
                     AGVFTTNQVIAAPCVITRKHLEGGTLRAIVANSGIANACTGEEGDRNAVAMARAAADA
                     VGCSPEEIGVASTGVIGWQLPIDRITKAVGEIRPTALGFPDFARAIMTTDTKPKQIVA
                     VASSGEHSGALRAIVTAKGAGMIHPNMATLLTFIVTDADIPLGALRPMVRRIADRSFN
                     AISVDGDTSTNDTLLVLANGASGVAATEGTLREAEAFLSDLAQRVAREIVADGEGVTK
                     VFAVHVSGAATDEDARRAARTITTSNLVKTAIHGADPNWGRIIAAAGRSGAKVDDRRA
                     SIRIGGIDVFREGTPVPFDPADLRKAFEAKDIDIELRLGLGEGSAIAWGTDLSAEYVH
                     INADYTT"
     gene            complement(4403..5425)
                     /gene="argC"
                     /locus_tag="VFV20_04170"
     CDS             complement(4403..5425)
                     /gene="argC"
                     /locus_tag="VFV20_04170"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEU5287575.1"
                     /translation="MATKVAIVGVTGYAGGELARILLRHPEVRIVAAVARSHQGEPLR
                     DVQQHLHGAPPSLVIGGDVGDAEVVFTALPAGEAAKQAGAWLGEGRRIVDIGSDFRLR
                     DPRAYEQWYRYTHPAPDLLADAVYGLTELVRPQLRKARVVANPGCYPTATLLALAPAL
                     RSKLIADDVIVDAKSGVSGAGHTVDEAYLFGTIDESVRPYGVPGHRHTPEIAQEAAVL
                     SGGAPRVTFTPHLVPMTRGLIASCYATLRDGVTAAQVMDAYATAYAAEPFVRITASFP
                     STKATLGGNWCLVHPVVDEPNRRLIVFGAIDNLVKGAAGSAVQNMNAMLGLPETAGLE
                     SLPLWP"
     gene            complement(5654..>5908)
                     /locus_tag="VFV20_04175"
     CDS             complement(5654..>5908)
                     /locus_tag="VFV20_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287576.1"
                     /translation="KVAIVGVTGYAGGVVVSYFEWVQSRAQFYWELDEVERRLEIYMR
                     RAMELVLSTAKTYDCSPREAAFIVAVDRVSRAIEKRGIFP"
BASE COUNT          816 a         2099 c         2082 g          911 t
ORIGIN      
        1 gagcaggtga tgggcgagcg agaccggctg cgcgcgctgg aggtgggtgt agccgggcag
       61 cactgtgtcg atctcctgct ccgcgcgctc gacgagcgcg agcgcgagcg acttcacagc
      121 atggtcgagc tcgtccagca gctcgatgac gagcaggcgc aggtcgagcg ccacctggtc
      181 gttgcggctc cgcgcggtgt gcagcttccc gccgaccccg ccggcgcggt cggtgagcac
      241 gcgctccacc gccgagtgca cgtcctcggc gtccgccggc caggggaagg tgccgtcgcg
      301 cagctcggcg gcgatgcgct cgagctcacg gaccagcatg gccgactcgt cggcggtcac
      361 gatgccctga cgcccgagca tccgcgcgtg cgcgatgctg ccgcgcacgt cgtgcagggc
      421 gatgcgccgg tcgaggtcga gcgacgccgt gaaggcgcgg atcttgggat cgagcgaccc
      481 ctcgaagcgc ccggaccaga gcggcttctc gctcatcgtc gcttcgcgcc gatgcgctta
      541 ggactgcttt tcgcaggggc tccgcccctt ccaccccgag gcccggagag ctccttcctc
      601 ggcacgtcga gcatggccgc ggtgccctcg ccccagaggg cctgcgtgcg ggcctgggtg
      661 cgcagggaca tgccgaagac cgtgatgaag ccctcggacg cgcggtggtc gaagaggtcg
      721 cccttgccat aggtggcgag agccgtcgag tacagcgagt gcggcgagcg acggccgacg
      781 atggtgaggt gcccgcggtc gaggcgaacg cggatctcgc cggtcacgaa gcgctgcgtg
      841 ctgcggacgt acgcggcgag gtcctgatgg tgcgccgtga accagagtcc gttgtagatg
      901 agctccgcgt actcgcgcgc gaccgcggat ttgaagcgca gggcctcctt ggtgagcacc
      961 atctcctcga gcgccttgtg cgccgcgtgc aggatcacgg cggccggcgc ctcgtagatc
     1021 tcgcgcgact tgatgccgac aagacggtcc tcgatctggt cgatgcggcc gacgccgtgc
     1081 cggccgccca gcttgttgag gcgctcgatg agcgccactc cgccgagctg ctccccgtcc
     1141 agcttcaccg gaacgccctc gctgaactcg atcacgagct cgcgcggctt cgccggggcc
     1201 tcgtctggat cgaccgtcca ctggaacacg tcgcgcgggg gcgcgcccca ggggtcctcg
     1261 aggacgcctg cttcgatcga gcggccccag aggttctcgt ccacgctgta cgggctcttc
     1321 ttcgtcgcct cgacggggat gccgcgcttt cgcgcgtagt cgatctcctc gtcgcgcgtc
     1381 atgttcatgc caccgcgcat cggcgcgatg accttgaggt ccggtgcgag ggcgccgacc
     1441 gcgaggtcga agcgcacctg gtcgttgccc ttgccggtgc agccgtgggc gacgtacttc
     1501 gcgccttcgc tgcgtgccgc gtcgacgagc agcttggcaa ggagcggacg gccgagcgcg
     1561 gtcgccagcg gatagcggtc ctgatacagg gcgtccgcct ggaggccggg ccacacgaag
     1621 tactcgacga agatgtcgcg cgcgtcctgg acgatggcct tgcgcgcacc gctggcgatc
     1681 gccttcttct gcaccaggtc gaggtccctg accgatccga ggtcgccggt gcaggtcacg
     1741 acctccaggc cgtacgtctc gcggagccac gcgaccgcga cggaggtgtc gaggccgccg
     1801 ctgtacgcca ggacgcaggt ttcgcctttc agactcttgg ccatgttcta gctccgttcc
     1861 ttcctggcga tcccgaacgc cgtcaagcgc gtgagcagcg agcgcgcgtg ccgctccgtg
     1921 gaggtcgcga cgaagacggt gtcgtcgccc gcgaccgtgc cgatgatctc cgcgaagtca
     1981 gacgtgtcga gcgcgatcgc catcgcgttc gcggtgccgg gcggcgagcg cagcacgacg
     2041 atgttgccgg cgaggcgtcc ctccaccaga tagtcctcgc agaatttgcg cagacggtcg
     2101 gtccccccgc cactcgcgac tgcggtcggc gccgcgtacg cgtgcgtccc gtcgcgtgac
     2161 accttcacga gcccgagctc gacgatgtcg cgcgagacgg tcgcctgcgt cgccacgaac
     2221 cccgcgttct cgagagcgcg aacgaggtcg ccctgcgtcg agagccggtg ctgctgcacg
     2281 aggcgcagca gcgcctgctg ccgcttgtgc tttcgcgcga gctccttcat gccgtgaaca
     2341 ccgtgccggc gcgctcgccg gccaggagcc gctcgatcgc gtcggcggag cgggcgtcga
     2401 cgatggccgc gcgacagccg gctcccgccg cgatgaggca cgcctcgacc tttggcagca
     2461 tgccgccctc gatggtgccg tcgtccacga gcgccttggc cctgtcgcgg tcgagcgtgg
     2521 ggatgacctt gccgtccttg ccgcggacgc ccgggacgtc ggtgatgaac acgaggagcc
     2581 gcgcgccgag gctcgcggcg atcgcgcccg cggcgctgtc cgcgttgacg ttcagcacct
     2641 ccacctcgcc ctcggcccgc tcctcgaagg cggccggcgc caccacgggc acgcgcccgg
     2701 cgtcgaccag ctcctcgagc agcgcgctgt ccaccttggt gatgcggccc acgaggccga
     2761 gttccggatc ctcccggatc gcgcggagga tgccgccgtc gatgccggag agcccgaccg
     2821 ccggcacggc gcgcgccgtc agcgcggcga cgatccgcgc gttcgcgagc cccgcgagca
     2881 cggcgagcgc gacgtcgcgc atcggctcgt cggtgacgcg caggccgcgc acgaagcgcg
     2941 tctgctgtcc gaggcttgtg gcccactccg acaccagcgg accgccgccg tgcacgacca
     3001 cgagggcatg gccggcgtcc tcgagtgcgg cgatgacgtc gagcgccgcg gcgtcctcac
     3061 ccgcgaccga gccgccgatc ttgaccacga tcaggtcgct catgtccgct ccgcggccat
     3121 gcgcagagga cctgtttctc gcaggggctc cgcccctgcc atcccggtgt cgtgccacac
     3181 gatgctcatg acattcacgg cgtgcattac gtcgtgtagt ccgcgttgat gtggacgtac
     3241 tccgcggaga ggtcggtccc ccacgcgatc gccgagccct cgcccaggcc gaggcgcagc
     3301 tcgatgtcga tgtccttcgc ctcgaacgcc ttgcgaagat ccgcggggtc gaagggcacc
     3361 ggggtgccct cgcggaagac gtcgatgcct ccgatgcgga tgctggcccg gcggtcgtcc
     3421 accttcgcgc cggagcgccc ggccgcggcg atgatgcgtc cccagttcgg gtcggcgccg
     3481 tggatggccg tcttcaccag gttcgacgtc gtgatcgtgc gcgccgcgcg cctcgcgtcc
     3541 tcatcggtcg cggcgccgct cacatgcacc gcgaagacct tggtcacgcc ctcaccatcg
     3601 gctacgatct cgcgcgccac cctctgcgcc aggtcgctga ggaacgcctc ggcctcgcgc
     3661 agcgtgccct cggtcgccgc cacgcccgac gctccgttcg cgagcacgag cagcgtgtcg
     3721 ttcgtgctcg tgtcgccgtc gacgctgatc gcgttgaagg agcggtcggc gatgcggcgg
     3781 accatcggac ggagggcgcc gagcgggatg tcggcgtcgg tcacgatgaa ggtgagcagc
     3841 gtcgccatgt tcgggtggat catccccgca cccttggccg tcacgatcgc gcgaagggct
     3901 ccagagtgct cgcccgagct cgcaaccgcg acgatctgct tcggcttcgt gtcggtggtc
     3961 atgatggcgc gcgcgaagtc ggggaagccg agcgcggtgg gccgtatctc gccgaccgcc
     4021 ttggtgatgc gatcgatcgg gagctgccag ccgatgacgc cggtgctcgc gaccccgatc
     4081 tcctctggcg agcagcccac cgcgtccgcg gcggcccgcg ccatcgcgac cgcgttgcgg
     4141 tcgccttcct cgccggtaca ggcgttcgcg atgccggagt tcgccacgat cgcgcgcagc
     4201 gtgccgccct cgaggtgctt ccgcgtgatg acgcacggcg ctgcgatgac ctgattcgtc
     4261 gtgaagacgc ccgcggctac gcacggccgg tcggacacga tgaggccgac gtcgagccga
     4321 tcggcgacgc cggtcttgac gccggccttc gccgcccccg cggtgaaccc gcgcgggacg
     4381 gagtcgctgt ggcggtcttc cttcacggcc acagcggaag cgactcgagc ccggcggtct
     4441 ctggcaggcc gagcatcgcg ttcatgttct gcaccgccga gcccgccgca cccttcacca
     4501 ggttgtcgat cgcgccgaag acgatgagcc ggcggttcgg ctcgtcgacg acgggatgga
     4561 cgaggcacca gttgccgccg agcgtggcct tcgtcgaagg gaacgacgcg gtgatgcgta
     4621 cgaatggctc agctgcgtac gcggtcgcgt acgcgtccat gacctgcgca gcggtcacgc
     4681 cgtcgcgcag cgtcgcgtag cacgacgcga tgagcccgcg cgtcatcggg accaggtgcg
     4741 gcgtgaacgt gacgcgcggc gcgccgcccg acaggacggc cgcctcctgc gcgatctcag
     4801 gcgtgtgccg gtggccgggc acgccgtacg gccgcacgct ctcgtcgatg gtgccgaaga
     4861 ggtaggcctc gtcgaccgtg tggccggctc ccgagacacc cgacttcgcg tcgacgatca
     4921 cgtcgtcggc gatcagcttc gagcgcagcg cgggcgcgag cgcgaggagc gtcgccgtcg
     4981 ggtagcagcc cgggttcgcg accacgcgtg ccttgcgcag ctgcgggcgg acgagctcgg
     5041 tcagtccgta gacggcgtcg gcgagcaggt ccggcgcggg atgcgtgtac cggtaccact
     5101 gctcgtacgc cctgggatcg cgcaggcgga agtccgagcc gatgtccacg atgcgccggc
     5161 cctcgccgag ccacgccccg gcctgcttcg cggcctcccc cgccggcagc gcggtgaaca
     5221 cgacttcggc gtcacccacg tcaccgccga tgaccagtga aggcggcgcg ccgtgcaggt
     5281 gctgctggac gtcgcgcaga ggctcgccct gatgcgaccg cgcgaccgcc gcgacgatcc
     5341 gcacctccgg gtggcgcagc aggatgcgcg cgagctcgcc gcccgcgtag ccggtgacgc
     5401 cgacgatcgc gaccttcgtt gccatgagcg gtcgattatg cgggtgaacg catatttatg
     5461 caagtcgtgg tcgagccccc atgcgacggc gcccgacgag gtcgagggag ggggccgatg
     5521 ctccgacggc tgatggtccg tgttgacctc gaaggacggg cgttccctga tcgctagtag
     5581 ccaggaccca gcgaggagga cgcatggccc ccgaccgagg caacgtcggc gggcgccgtc
     5641 gtatgggccc gacctagggg aagatccccc gcttttcgat cgcccgcgac acacggtcga
     5701 cggcgacgat gaacgcggct tcgcgcggcg agcagtcgta ggtcttggcg gtcgagagga
     5761 cgagctccat cgcacggcgc atgtagatct ccaggcggcg ctcgacctcg tcgagctccc
     5821 agtagaactg cgcccggctc tggacccatt cgaagtacga gacgacgacg ccgcccgcgt
     5881 agccggtgac gccgacgatc gcgacctt
//
LOCUS       DASTZR010000189         2686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_898643, whole genome shotgun sequence.
ACCESSION   DASTZR010000189 DASTZR010000000
VERSION     DASTZR010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898643"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VFV20_04180"
     CDS             complement(<1..442)
                     /locus_tag="VFV20_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287577.1"
                     /translation="MFSFASMDRYELAWAAGFFDGEGWAASARFGRGEQRRPQARVNQ
                     AGTDGVPEVLTRFRAALQAGRLGGPYRKTGRRDMYCWEAASRNDVELIHHLLAPWLGQ
                     VKLLQFATALARASLHAREARPDDEWRSWAAGLYDGEGCSALLRH"
     gene            complement(547..864)
                     /locus_tag="VFV20_04185"
     CDS             complement(547..864)
                     /locus_tag="VFV20_04185"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEU5287578.1"
                     /translation="MPLFTRAQAEALLPKARPLLEDLQRRVISYRRRPSEPLVREIEV
                     LLREVNDLGIEVKDPGRGLIDFRAMLRGREVYLCWQLGEGERISYWHDLETGFAGRRI
                     IED"
     gene            935..1231
                     /locus_tag="VFV20_04190"
     CDS             935..1231
                     /locus_tag="VFV20_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEU5287579.1"
                     /translation="MATYVLLTKVTPAGVKTIQSNPRRIKEVNKEIETLGARIVAQYA
                     TLGRYDFVNIVEAKDSEAIARIAVALGARGTVSIETLTAIPIETFIRTVGRKGK"
     gene            complement(1241..1540)
                     /locus_tag="VFV20_04195"
     CDS             complement(1241..1540)
                     /locus_tag="VFV20_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287580.1"
                     /translation="MGIAFLADLARQRSLASAALLFLMLIDEIEGLVFVALGVVMHRI
                     DLFLGVQDVLFPTIVIVAGVVSLARNWRRRAPAEPLPAPETLELAVLSQRIGSTR"
     gene            complement(1618..2406)
                     /gene="lipB"
                     /locus_tag="VFV20_04200"
     CDS             complement(1618..2406)
                     /gene="lipB"
                     /locus_tag="VFV20_04200"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEU5287581.1"
                     /translation="MSASVGNVADPLVATWLGRVDYLEGWRLQDAVASRVRAGGDERL
                     LLLEHDPVYTIGRRGTLEHLLAGPSELRAAGASVYRVDRGGDITYHGPGQLVGYPIVR
                     LGERADLVRYVRGLETALIHVLADYGIAARPERGKTGVWVDLPDGRPAKIAAIGVRVS
                     RGVTTHGFALNVSTDLTAFARMVPCGFMHEVASFERLGVWTEVREVGTRVAVALSAEL
                     GRLPALGGVEPARDVEIAADAPVRSAVDLVGQRHSGLTAGSTTA"
     gene            complement(2387..>2686)
                     /locus_tag="VFV20_04205"
     CDS             complement(2387..>2686)
                     /locus_tag="VFV20_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEU5287582.1"
                     /translation="IAAHGQGIVCKTGIMVGLGETMDEVLATMRDIVAQGTHVLTVGQ
                     YLRPSPMHLPIERYWTPEEFAAIRDAGMSMGFRHVESGPLVRSSYHAERHVGLGR"
BASE COUNT          464 a          953 c          908 g          361 t
ORIGIN      
        1 ggtgacgaag aagggcgctg cacccctccc cgtcatacag accggccgcc cagcttcgcc
       61 actcatcgtc cggccgcgcc tcgcgcgcgt gaagagacgc tcgtgccaga gcggtcgcga
      121 actgcagcaa cttgacctgg ccgagccacg gcgcgagcag gtgatggatc agctcgacgt
      181 cgttccggct cgcggcctcc cagcagtaca tgtctcgacg ccccgtcttc ctatacggtc
      241 ctccgagtcg cccggcctgc agtgccgcgc ggaagcgagt caggacctcc ggcacgccat
      301 cggtccccgc ctggttcacg cgcgcctggg gccgccgctg ttcgccacga ccgaagcgcg
      361 ccgacgcggc ccaaccctcg ccatcgaaga atcccgccgc ccacgccagc tcataccgat
      421 ccatcgacgc gaaagagaac acatgagcga ggctcgcgtg aaggccgcgc gcgtcacatg
      481 ttcatgcgac gacgcgtcac gatttcatgc gacagacgcg cgtcgcacgc tcatgcgacg
      541 cgcaggctag tcctcgatga tcctccggcc cgcgaatccc gtttcgaggt cgtgccagta
      601 ggagatgcgc tccccttccc cgagctgcca gcagagatag acctcgcgac cgcgcagcat
      661 cgctcggaag tcgatgagcc cccggcccgg atccttcacc tcgatgccga ggtcgttgac
      721 ctcgcggagg agcacctcga tctcgcgcac gagcggttcg ctcgggcggc gccggtagct
      781 gatgacgcga cgctggaggt cctcgaggag cggcctcgcc ttcggcagca gcgcctcagc
      841 ctgagcgcga gtgaacagcg gcatatgctc tcgccgagta tcgcgcgtgc gggcccgatg
      901 ggccgtgcgg ctcgatcggc cgaagggaga cgacatggcg acctacgtcc tgctcacgaa
      961 ggtgactccg gcgggggtca agacgatcca gtccaatccg cggcggatca aggaggtcaa
     1021 caaggagatc gagacgctcg gggcacgcat cgtcgcgcag tacgcgaccc tcggccgtta
     1081 cgacttcgtg aacatcgtcg aggcgaagga cagcgaggcc atcgcccgca tcgcggtggc
     1141 actgggcgcg cggggcacgg tgtcgatcga gacgctcacc gcgataccga tcgagacgtt
     1201 catccggacg gtggggcgta aggggaagta gcgcctcggg ctagcgcgtc gatccgatcc
     1261 gctgcgagag cacggcgagc tcgagcgtct cgggcgccgg aaggggctcg gcaggcgcgc
     1321 gtcggcgcca gttgcgcgcg agcgacacga cgcccgccac gatcacgatc gtcgggaaga
     1381 ggacgtcctg cacccccagg aagaggtcga tgcggtgcat gaccacgccg agcgcgacga
     1441 acacgagccc ttcgatctcg tcgatgagca tgaggaacag cagcgccgct gacgcgagcg
     1501 agcgctgacg tgcgaggtcg gcgaggaaag cgatacccac ccaccatgac cctaggcgcg
     1561 cgaggaagcc tccggcgata gggccatttg ggtcactcga tggatgggtc gcccggccta
     1621 ggcggtagta ctgcccgcgg tcaggccgga atggcgttgc cccacgaggt cgacggcgga
     1681 acgcacgggc gcatccgcgg cgatctccac atcgcgtgcc ggctccactc cgcccagcgc
     1741 cgggaggcgt ccgagctcgg ccgagagcgc cacggccacg cgcgtcccga cctcgcggac
     1801 ctccgtccac accccgagcc gctcgaaaga agccacctcg tgcatgaagc cgcacggcac
     1861 catgcgtgcg aacgcggtga gatcggtgga cacgttgagc gcgaagccgt gcgtcgtcac
     1921 gccgcgcgag acgcggacgc cgatggccgc gatcttcgca gggcggccgt ccggcagatc
     1981 gacccacacg ccggtcttgc ctcgctcggg acgggccgcg attccgtagt cggcgaggac
     2041 gtgaatgagc gccgtctcga gaccgcggac gtaccgcacc agatcggccc gctcgcccag
     2101 acgaacgatc gggtagccga cgagctgccc gggtccgtgg taggtgatgt cgccccctcg
     2161 gtccacgcga tagaccgacg cgcccgccgc gcgcagctcg gacggacccg cgagcaggtg
     2221 ctcgagcgtg ccgcggcgcc cgatcgtgta gacggggtcg tgctcgagca ggagcagacg
     2281 ctcgtcgccg cccgcgcgca cgcgcgacgc gaccgcgtcc tggagccgcc aaccctcgag
     2341 gtaatccacg cggccgagcc aggtcgcgac gagcgggtcg gcgacgttac cgaccgaggc
     2401 cgacatgacg ctccgcgtga tacgacgagc gcacgagcgg accggactcc acgtgacgga
     2461 agcccatcga catgcccgca tcgcggatcg cggcgaactc ttccggcgtc cagtagcgtt
     2521 cgatcggcag gtgcatcggc gaaggccgca ggtactgccc caccgtcagg acgtgcgtgc
     2581 cctgcgcgac gatgtcccgc atcgtggcga gcacctcgtc catcgtctcg ccgaggccga
     2641 ccatgatccc cgtcttgcag acgatcccct ggccgtgcgc cgcgat
//
LOCUS       DASTZR010000190         5160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_246090, whole genome shotgun sequence.
ACCESSION   DASTZR010000190 DASTZR010000000
VERSION     DASTZR010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5160
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246090"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1588
                     /locus_tag="VFV20_04210"
     CDS             137..1588
                     /locus_tag="VFV20_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5287583.1"
                     /translation="MAATRVIDTLLTGELAPIRAELEKRHDESIKRLQNWVRHPSIAA
                     EDRSMDEGCDLMMRMARDAGFQQAKRIDTDGHPAVFATLDAGAPRTFGLYFMYDVKQA
                     DPAEWSSPPFDAAIVEKPGFGKVVMGRGAVNQKGPESAFLAALHAIHGAGMKLPINLV
                     LVAEGEEEIGSPHIRQVTGHPEVAAALKKTVGVVMPAAWQDPDGNVTVYLGAKGVIEV
                     ELVASGERWGRGPGIDVHSSNRARLDSPAFHLVQALATLVNADGDPAIEGFGREAQPA
                     TSEQKAMLETAAGRISEQTAKRILATKRWAHDEGWRESLERFMFTPTVNIEGLVAGYT
                     GPGGKTILPHRAVAKLDLRLVPDMTYDGALAQLRAHLANHGFGDIEVNPSGGYDPTET
                     PLDAPLIQAQLAVLGRAGIEPAVMPRIAGSYPGYAFTSAPLRLPSGHFGFGHGSGAHA
                     PDEYFVIEPSNPRVSDWDGAVASHVAYLFALAS"
     gene            complement(1607..4489)
                     /locus_tag="VFV20_04215"
     CDS             complement(1607..4489)
                     /locus_tag="VFV20_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287584.1"
                     /translation="MRTRRTVLKAVVPLVVSACTPGAQGRADETTPPPLDTSGGSGVA
                     PAAVRFGGTGYTPFGEWRTDVVVSAGRWVPGIPLRVDATLTIREAHLAQLAAAVKAKI
                     DGFVALVTAERTFDADGILRLPSDERMSTLLTVTSIGIEGGTQGAVSKRVGYAFGTPV
                     DELATVPLAAATNTAEGHDAAFSISTRLAKELPPGIYRLRIDYGVAIGKRVVNLSGEP
                     FAKRGFPKGRDVESEIYSPPIPASGPHTSGRMVDATAIRPRVPWVLLAAYNSNGYRGV
                     VADEDAPHFALSSRNIMQDDVILPRFADHAGKVVAAYSLEPAFPADAICARQNIPWEP
                     AKGELSVRITQPDGTTVDLGSAPFVARTPLGLTTKNAKFTQWKPPAYGRYTVRATGWT
                     QDAWGNHYEGGGTYSFWIAKRMTLATATFQGQAYPVGNRYGRDMAFSPAVPAHVDVTA
                     TLFANSDPAQARTVSYSGTASAGGMFTANEGLKPLVFDAPGEYQAKVLATYTDRDGHL
                     WASTMRHAGIVYPADTPIVARGKKLKVKDQLVTRGETHAEGWIDTKTEETHLEHVNFP
                     YQAGDVLLIAAEYQGANKIEPVLTWDDRRAPFTYDPAWQAIGITNVRIQTSNGYSPHL
                     FPEYITDLLYYYGAAPRPGFMGRFLVGEDGVRAPYWPTTNTNFGGQINASSNGDAPGH
                     IYRLIGGVVVRRHGETPLYAGYMATAFILPKGSKNNRVIAPGSEDLVGSTRERARIFL
                     AMNARPGILYETGTTFAPAFQIDPMLPVNMRFTMRYPDGRSVSASGVADAGGSWVGGE
                     RWPLDVPGLYRYSVDAQWSGHKGLVPGMPQDGGAIYVVEKERPAGAPGITFALPPQST
                     FDAVKGATFTASTTAREVHYAMITPGAVLAQGMIPVQNGKFSFLFDAASLNERAQTYE
                     VVNQVTGKPELGQVVHLTFFSKETSPTTYHSFVRLIIRGNTVLYTR"
     gene            4685..4807
                     /locus_tag="VFV20_04220"
     CDS             4685..4807
                     /locus_tag="VFV20_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287585.1"
                     /translation="MTPDAATVALTLLGLALGVAASVGAGMAAARLLFAAAQDR"
     gene            4882..>5160
                     /locus_tag="VFV20_04225"
     CDS             4882..>5160
                     /locus_tag="VFV20_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287586.1"
                     /translation="MPLRLMVMHIVRPRGLLLILLAAVLIIGCAAPAGLPGASPARSF
                     PLLASGSYVYQPRPFVWQVVTIRADAVTGGAGQVYEWSCAGQRWAIGSI"
BASE COUNT          737 a         1782 c         1834 g          807 t
ORIGIN      
        1 cgccgagcgt gcacgcgagc aagaggaacg gctcgcgcgc gggtgggccg aggccgaaga
       61 gcgcgcgaag cagcagctgg cggcgatctt ctcatcggct ggcatctgac tcccacggca
      121 ggcatcatgt cctgccgtgg ccgcgacccg cgtgatcgac acgctcctca ccggcgagct
      181 cgctccgatc cgcgctgagc tcgagaagcg ccacgacgag agcatcaagc ggctccagaa
      241 ctgggtgagg catccgtcga tcgcggcgga agaccggagc atggacgaag gctgcgatct
      301 gatgatgcgc atggcgcgcg acgcggggtt ccagcaggcg aagcgcatcg acaccgacgg
      361 tcatcccgcg gtcttcgcga cgctcgacgc cggcgcgccg cgcaccttcg gcctctactt
      421 catgtacgac gtcaagcagg ccgaccccgc ggagtggtcg tcgcctccgt tcgacgcggc
      481 catcgtcgag aagccgggct tcggcaaggt cgtcatggga cggggcgcgg tgaaccagaa
      541 gggtcctgag tcggccttcc tcgcggcgct ccacgccatc cacggtgcgg gcatgaagct
      601 accgatcaac ctcgtcctcg tcgccgaggg cgaggaggag atcggatcgc cacacatccg
      661 ccaggtgacc ggtcatcccg aggtcgccgc cgcgctgaag aagacggtcg gcgtggtcat
      721 gccggccgcg tggcaggacc ccgacgggaa cgtgaccgtg tacctcggcg cgaagggcgt
      781 gatcgaggtc gagctcgtcg cgagcggcga gcgctgggga cgcggtcccg gcatcgacgt
      841 ccattcgtcg aaccgcgcgc ggctcgacag tcccgcgttc catctggtcc aggcgctcgc
      901 gacgctcgtg aacgccgacg gcgatccggc gatcgaaggc ttcggacgcg aggcgcagcc
      961 cgccacgtcc gagcagaagg cgatgctgga gacggcggcc gggcgcatct ctgagcagac
     1021 cgcgaagcgc atcctcgcca cgaagcgctg ggcgcacgac gaggggtggc gcgaatcgct
     1081 cgagcgcttc atgttcacgc cgaccgtgaa catcgagggc ctggtcgcgg ggtacaccgg
     1141 tcccggtgga aagacgatcc ttccgcaccg tgcggtggcg aagctcgacc tccggctcgt
     1201 tcccgacatg acgtacgacg gcgcgctcgc gcagctgcgc gcgcacctcg cgaatcacgg
     1261 cttcggcgac atcgaggtga acccgagcgg cggttacgac ccgacggaga cgccgctcga
     1321 cgcgccgctc atccaggcgc agctcgcggt gctggggcgc gcgggcatcg agccggctgt
     1381 gatgccgcgc atcgccggga gctaccccgg ctacgcgttc accagcgcgc cccttcggct
     1441 cccatcgggg catttcggat tcgggcacgg cagcggcgcc cacgcgccgg acgagtactt
     1501 cgtgatcgag ccgtcgaacc cgcgcgtgag cgactgggac ggcgccgtcg cctcccacgt
     1561 cgcgtacctc ttcgccctcg cgagctgagc tcgcgcgcgg ccggcgtcag cgcgtgtaga
     1621 ggacggtgtt cccgcggatg atgagccgca cgaaggagtg gtacgtcgtg ggcgacgtct
     1681 ccttcgagaa gaacgtcagg tgcacgacct ggccgagctc cggcttcccg gtcacctgat
     1741 tcacgacctc gtacgtctgt gcgcgctcgt tgagcgaagc ggcgtcgaag aggaacgaga
     1801 acttcccgtt ctgcaccggg atcatgccct gcgcgagcac cgcgccgggc gtgatcatcg
     1861 cgtagtggac ctctcgcgcg gtcgtcgacg ccgtgaacgt cgcgcccttg acggcgtcga
     1921 acgtcgattg cggtggtagg gcgaacgtga tgcccggcgc gcccgcgggc cgctccttct
     1981 cgacgacgta gatcgcgcct ccgtcctgcg gcatgccggg gacgaggccc ttgtgcccgc
     2041 tccactgggc gtccacgctg tagcggtaga ggccgggcac gtcgagcggc catcgctcgc
     2101 caccgaccca cgatccaccg gcgtcggcca cgccggacgc gctgaccgag cgcccgtccg
     2161 gatagcgcat ggtgaaccgc atgttcaccg gcagcatggg atcgatctgg aaggccgggg
     2221 cgaaggtcgt cccggtctcg tagaggatgc cggggcgggc gttcatcgcg aggaagatgc
     2281 gcgcgcgctc gcgcgtcgag cccacgaggt cttccgagcc cggcgcgatc acgcgattgt
     2341 tcttcgagcc cttcgggagg atgaacgcgg tcgccatgta tcccgcgtag agcggcgtct
     2401 cgccgtgccg tcgcaccacg acgccgccga tgagacggta gatgtggccc ggcgcgtcgc
     2461 cgttgctcga cgcgttgatc tgtccgccga agttcgtgtt cgtcgtcggc cagtagggag
     2521 cgcgcacgcc gtcctcgccg acgagaaagc ggcccatgaa gcccggccgc ggcgcggcgc
     2581 cgtagtagta gaggaggtcc gtgatgtact cggggaagag gtgcggcgag tacccgttcg
     2641 aggtctggat gcggacgttg gtgatcccga tcgcctgcca tgccgggtcg tacgtgaagg
     2701 gcgcgcgcct gtcgtcccag gtcagcaccg gctcgatctt gttcgcgccc tggtactcgg
     2761 ccgcgatgag cagcacgtct cccgcctggt acgggaagtt gacgtgctcg aggtgggtct
     2821 cctcggtctt cgtgtcgatc cagccctccg cgtgcgtctc gccgcgcgtc acgagctggt
     2881 ccttcacctt caacttcttg ccgcgcgcga cgatcggcgt gtccgccggg tacacgatcc
     2941 ccgcgtggcg catggtcgag gcccagaggt ggccgtcccg gtcggtgtag gtcgcgagga
     3001 ccttcgcctg gtactcgcct ggcgcgtcga acacgagcgg tttcaggccc tcgttcgcgg
     3061 tgaacatgcc gcccgccgat gccgtgccgg agtaggacac cgtgcgcgcc tgcgccggat
     3121 cggagttcgc gaagagcgtc gcggtcacgt cgacgtgcgc cggcacggcc ggcgagaacg
     3181 ccatgtcgcg gccgtagcgg ttgcccacgg gatacgcctg gccctggaac gtcgcggtgg
     3241 cgagcgtcat ccgcttcgcg atccaaaagg agtacgtgcc gccaccctcg tagtggttgc
     3301 cccacgcgtc ctgcgtccag ccagtcgccc tcaccgtgta gcgcccgtag gcgggcggct
     3361 tccactgcgt gaacttcgcg ttcttcgtcg tgaggccgag tggtgtcctg gccacgaatg
     3421 gcgcgctgcc gagatcgacg gtggtgccgt ccggctgcgt gatccgcacc gagagctcgc
     3481 ccttcgcggg ttcccatggg atgttctgcc gcgcgcaaat cgcgtcggcg gggaaggccg
     3541 gctcgagcga gtacgccgcg acgaccttcc cggcgtggtc ggcgaagcgg gggaggatga
     3601 cgtcgtcctg catgatgttg cggctcgaga gcgcgaagtg cggcgcgtcc tcgtccgcga
     3661 cgacgccgcg atacccgttg gagttgtacg cggcgagcag gacccacggg acgcgcggcc
     3721 ggatcgcggt cgcgtcgacc atccgtccgc tcgtgtgcgg gccgctcgcg gggatgggcg
     3781 gcgagtagat ctcggattcg acgtcccgtc ccttcgggaa cccgcgcttg gcgaatggct
     3841 cgccgctcag gttcaccacg cgcttcccga tcgcgacgcc gtaatcgatg cgcaggcggt
     3901 agatgcccgg cgggagctcc ttcgcgagtc gcgtcgagat gctgaacgcc gcgtcgtgcc
     3961 cttccgcggt gttcgtggcc gcggcgagcg gaacggtcgc gagctcgtcg accggcgtcc
     4021 cgaacgcata ccccacgcgc ttgctgaccg cgccctgcgt gccgccctcg atgccgatgg
     4081 acgtgacggt cagcagcgtg gacatgcgct cgtcgctcgg taggcgcagg atgccgtccg
     4141 cgtcgaacgt gcgctccgcg gtgacgagcg cgacgaaccc gtcgatcttc gccttcacgg
     4201 ccgccgcgag ctgcgcgagg tgcgcctcgc ggatggtgag cgtcgcgtct acgcgcagcg
     4261 ggatcccggg gacccaccgg ccggcggaga cgacgacgtc cgtgcgccac tcgccgaacg
     4321 gcgtgtatcc ggtgccgccg aagcgcaccg cggccggcgc gactccgctg ccacccgatg
     4381 tgtccagcgg gggaggggtg gtctcatccg cgcgtccctg ggcgccgggc gtgcacgccg
     4441 agaccacgag cgggacgacc gccttcagga ccgtgcgccg cgtgcgcaac acgtacgaca
     4501 tcgtgcgtcc ggctgagaag cgaacgatgt ccgacccgcg tccggtctgt gaaagaaccg
     4561 tcgtttccgc ggcggaggtc atcgccgccg gcgccgtccg gcccaccgcg gctcaggctg
     4621 agcccggtcc tcagccgacg ttcggcactg gggcccgcgc gcgcgcgagt cgaagatgag
     4681 ccgcatgaca ccggacgccg cgaccgtcgc gctgacgctc ctcggtctcg cgctcggcgt
     4741 cgccgcgtcc gtcggcgcgg gcatggccgc cgcgcgactc ctgttcgccg cggctcagga
     4801 tcgctagcga gcgacacgcg gtccgccttc gcagcgtcgc gaacgacaca ctgcgaacgc
     4861 gtcggacgcg gaacccgcga catgcccttg cgtcttatgg tcatgcacat cgtcagacca
     4921 cgaggcctgc tcctcatcct gctcgccgcg gtgctcatca ttggttgcgc cgcgccggca
     4981 ggtcttccgg gtgcgtcgcc ggcgcgctcg ttcccgctgc tcgcttcggg gtcgtatgtc
     5041 tatcagccgc ggcccttcgt ctggcaggtc gtgacgatcc gcgcggacgc cgtgaccggc
     5101 ggtgcgggcc aggtgtacga gtggagctgc gcgggacagc ggtgggccat cggcagcatc
//
LOCUS       DASTZR010000191         7096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1209708, whole genome shotgun sequence.
ACCESSION   DASTZR010000191 DASTZR010000000
VERSION     DASTZR010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7096
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209708"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..415)
                     /locus_tag="VFV20_04230"
     CDS             complement(62..415)
                     /locus_tag="VFV20_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287587.1"
                     /translation="MRPPSLWLLGFAVGIAGIPMASYVAFPGMIVLGLLALALVRSRS
                     VALFGGLSLAVGAWFSFLEWRTVANCDAFNRRGGGSCTIIDPAPSVLIPLLFLALGVA
                     LSAYGLLRDRPREAA"
     gene            448..1224
                     /locus_tag="VFV20_04235"
     CDS             448..1224
                     /locus_tag="VFV20_04235"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013615677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEU5287588.1"
                     /translation="MSEKSIVQLFDLADKVAVVTGAGSGLGLGIARRLAEAGAGVVLN
                     DIDAGRAKAAAGRLRGRGYSMLEPVASDVSTPEGAKAAIDAAVTRFGRIDILVNNAGI
                     YPTSPFLEHPVELWDQVVDVNLRGPFLCAQAAARQFEKQGKGDAILNIASVVGMVGSG
                     PAAIAYTASKGGLIALTKTLARAVGPFGARANAIAPAAIETESARGMLDRSSLARQPV
                     GRFGTPDDVALAALYLVAPASDYVTGEVLVVDGGYLLVQA"
     gene            1493..4921
                     /locus_tag="VFV20_04240"
     CDS             1493..4921
                     /locus_tag="VFV20_04240"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5287589.1"
                     /translation="MPVYVVVGDLVAAVVWMAASAPNERSIAFVVVLGIGALAMFRLG
                     GSGVALTGGGYLVGRIAQEWIRVSLGIPTPVPQIVAEVIVVSLVLMILAATVGHYRAE
                     QARGARALRLARSLERIASEVGEVTDPAHLLRTITSSAMQLVEADHATINRRHGDEFF
                     VAEGSGTGARAIGIHAPSGHGIVGAVVARRATVALDDYRIDPTAVPAIKDLGVRSIVG
                     VPIILQGEIVATLTVARLAVRPFDEEDQRALEGLASHAAVALRNARLLELSRRLEALS
                     REVAGDTGALDDVMRRVANDIAAAYDAEVVTISLVKGDLSRPIAHVGLTPDEAPRLST
                     PGPMTRLVIARRESIVVRDYDAELGTDPHVMPALARRSVHASVGAPVIVQGEVVAAVM
                     VGTTDPYRSFDAIDRQGLTAFAQTTGAALRSAQARQERERRIERLSALNVLAWQLAGV
                     HEPYEIARLAYEAAGGLVARDAFYVARFDHEKREFDFVLQADGEDVWRGERYPLATGP
                     TSQVVVTGETYVVRSPEDPAQRRGMTFGEATRVSGSAAHVPLKSRGRLIGVLSSQSYS
                     SDAFDDEDIVVLQSLANLVATAFENAEHMQQMRELYLASVKALAAAVDARDPYTRSHS
                     ARVAALARTVAEELRLGPDELRRVQLGALLHDIGKIGIPDAILNKPGPLSADEWVLMR
                     THPAVGASILAAVEPLRDLVPIVRTHHERYDGNGYPEHLAGDEIPIESHIVAAADAFE
                     VIVSRRAYKQAQTVEFACAELLRCRATQFHPDVVDAFLRVIERDRQQGANYLRRVAGI
                     EHEEIENVPGPGGVLEQFAATAHAHGRQLAILQRLASEISAVLDIDELAGRLLRIVCE
                     SMGYDSGFLLTLDDRHTGLVVRAAVGPSEPYVGQVLPAGTSISWWVVEHGLPQLVVDV
                     QKDERYYGPPDINTVLCVPLQLGDERVGVLGIESRRAQAFGRDDQELLTAVSHQVAAA
                     LRVARLHQQAKHAAATDPLTSLPNRRTFFEQLRRELSGDPAQAVSIAVLDVNGLKERN
                     DEFGHVAGDEALVRIGEILTAGVRDGDLVARIGGDEFAILFPATTQLAAERVMRRLAD
                     CVRDGTVGDGLRLPTIAWGVADAATRTMTVDELVDAADRAMYRHKQKARRATTA"
     gene            complement(4940..5191)
                     /locus_tag="VFV20_04245"
     CDS             complement(4940..5191)
                     /locus_tag="VFV20_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287590.1"
                     /translation="MVRIYGKGNVLFALANPLLTAFARTVGGEPAFTPEERVLLQAER
                     DGQRLANEGYRVVGSQWYEVPILHVMYLRVVYERDATRG"
     gene            5512..>7096
                     /gene="valS"
                     /locus_tag="VFV20_04250"
     CDS             5512..>7096
                     /gene="valS"
                     /locus_tag="VFV20_04250"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEU5287591.1"
                     /translation="MANATTPPAAVLDFSEKNFRPSVVETELYDWWEHSGFFTPPAEP
                     SPGEKTFVMMLPLPNVTGDLHMGHALGFGGYEDLMARWHRMKGEATLYLPGTDHAGII
                     AQIVVEKQLAKEANPKALPRDQFLDEMWKYMRHYQPRIYKQLRMLGCSLDWTRVHFPM
                     DPDMQRRVRIHFIRLYKNGHLYRDNRIVHWCLTCQTTYSDLETLHVERTDQLSYVRYP
                     WADAMPAGTPDLVIATTRPETIVADTAIAVHPDDDRWKAYIGKDVLVPLVERRVKIVG
                     DEAVDPAFGTGALKVTPGHDATDFDIGQRHGLQVISAIDKRGTMMPSAGILAGAHRDA
                     ARAKAIEVLKQTGLLQKQEPITHAIAIHDRCSTVDEPLVLKQWWMRMKPLAKPAIDAV
                     RDGRIQIIPRYQEKIYFEWMENIRDWSIARQIYWGHSIPAWYCQDCDEIVVPDEDAPD
                     PTQCTKCRSDDLRRDPDVLDTWFSSALWPHVTLGWPEKTKDLERFYPGTMLETGWDIL
                     FFWVARMIMMGLKFTGEVPF"
BASE COUNT         1090 a         2512 c         2468 g         1026 t
ORIGIN      
        1 gtgtacgtcc cggcccgcac cgacatcgcc ggagcgcctg ccgagcgcgg ctgaccggac
       61 gctacgcggc ctctcgcggc cggtcgcgca gcagcccgta cgcgctgagc gcgaccccca
      121 aggcgaggaa cagcagcggg atgagcacgg acggcgccgg atcgatgatg gtgcaggagc
      181 cgccgccgcg gcggttgaac gcgtcgcagt tcgcgacggt gcgccactcg aggaacgaga
      241 accacgcgcc gacggcgagc gacagcccgc cgaagagcgc gacgctgcgg ctgcgcacga
      301 gggcgagcgc gagcaggccg agcacgatca tgcccggaaa cgcgacgtag cttgccatcg
      361 ggatcccggc gatgccgacg gcgaagccca ggagccacag gctcggcgga cgcatggttc
      421 cgtgagtctt cgtagactgg acctcccgtg tccgagaaat ccatcgtcca actcttcgac
      481 ctcgcggaca aggtcgccgt cgtgaccggc gctggctcgg gcctcgggct cggcatcgcg
      541 cgccggctcg ccgaagccgg ggcgggcgtg gtcctgaacg acatcgacgc cggtcgtgcg
      601 aaggccgcgg cggggcggct gcgcggtcgc ggctacagca tgctcgagcc tgtcgcgtcc
      661 gacgtgtcca cgcccgaggg cgcgaaggcg gccattgatg ccgcggtcac gcggttcgga
      721 cgcatcgaca tcctcgtgaa taacgccggc atctatccga cctcgccgtt cctcgagcat
      781 ccggtggagc tctgggacca ggtcgtcgac gtgaatctgc gcggtccgtt cctctgcgcg
      841 caggcggcgg cgcggcagtt cgagaagcag ggcaagggcg acgcgatcct caacatcgcg
      901 agcgtcgtcg gaatggtggg ctcggggccg gcggcgatcg cgtacaccgc gtcgaagggc
      961 ggactcatcg cgctcacgaa gaccctcgcg cgcgccgtcg gcccgtttgg cgcgcgcgcc
     1021 aatgcgatcg cgccggcggc catcgagacg gagagcgcac gcgggatgct cgaccgctcg
     1081 tccctcgccc gtcagccggt cggccgcttc ggcacgcccg acgacgtggc gctcgcggcc
     1141 ctctatctcg tcgcccccgc atccgactac gtcaccggcg aagtcctcgt cgtggacggc
     1201 ggctacctgc tcgttcaggc ctagaagcgg gggctttcgg gcggctgacc gcggactgat
     1261 acccgtccac aggaccgctt cctatgctgc tcgtacgccc tatcgcttcg tccccaggga
     1321 cgtcctgctg ctggcgcggc tactcgccgc cagcggtgcc accctgtggc tcgccgtcgg
     1381 cggcgccgcg gtcacgccgc tcgtcgtcgc cgcgctcggc ttcctcctcg tcacgtcggc
     1441 gctcatttgg cggaacgaca tccggctacg gcgcgcgcca tcgctgtccg aggtgcccgt
     1501 ctatgtcgtg gtcggcgacc tcgtcgccgc ggtcgtctgg atggccgcga gcgccccgaa
     1561 cgaacggagt atcgccttcg tggtggtgct cggcatcggt gcgctcgcga tgttccggct
     1621 cggcgggtcc ggcgtcgccc tgaccggcgg cgggtacctg gtgggacgga tcgcgcagga
     1681 gtggatccgg gtgtccctgg gcatccccac gcccgtgccc cagatcgtcg ccgaggtgat
     1741 cgtcgtcagc ctcgtgctga tgatccttgc cgccaccgtc gggcactacc gcgcggaaca
     1801 ggcgcgaggc gcgcgggcgc tccgtctggc gcgcagcctg gagcggatcg ccagcgaggt
     1861 gggcgaggtc accgatcccg cgcacctcct gcgcacgatc acctccagcg cgatgcagct
     1921 cgtggaggcc gaccacgcga ccatcaatcg tcgccacgga gatgagttct tcgtggcgga
     1981 gggctccggc accggcgcgc gggcgatcgg catccacgca ccgagcggtc acggcatcgt
     2041 cggcgccgtc gtcgcgcggc gcgccaccgt ggccctcgac gactaccgga tcgatccgac
     2101 ggcggtcccc gcgatcaagg acctgggcgt gcggtccatc gtgggcgtcc cgatcatcct
     2161 ccagggcgag atcgtcgcga cgctgacggt ggctcgcctg gcggtccgcc cattcgacga
     2221 ggaggaccag cgcgcgctcg aaggtctcgc gagccacgcc gccgtggcgc tgcgcaacgc
     2281 gcggctgctc gagctctccc gccgcctcga agcgctctcg cgcgaggtag cgggcgacac
     2341 cggtgcgctc gacgatgtca tgcgccgtgt ggccaacgac atcgccgcgg cgtacgacgc
     2401 ggaagtcgtg acgatctcac tggtgaaggg cgatctgtcg cgcccgatcg cgcacgtcgg
     2461 cctcaccccc gacgaggcgc ctcgactctc gacgccgggg cccatgacgc ggctcgtgat
     2521 cgcccggcgc gagtcgatcg tcgtgcgcga ctacgacgcc gagctcggta cggatccgca
     2581 cgtgatgccc gcgctggcgc ggcgctcggt gcacgcgtcg gtcggtgccc cggtcatcgt
     2641 ccagggtgag gtcgtggcgg ccgtcatggt cgggaccacc gacccctatc gctcgttcga
     2701 cgcgatcgac cgccaggggc tgacggcatt cgcccagacg accggcgcgg cgctgcggtc
     2761 ggcgcaggcg cggcaggagc gcgagcgccg catcgagcgg ctctcggcgc tgaacgtcct
     2821 cgcgtggcag ctggcgggcg tgcacgagcc gtacgagatc gcccgcctcg cgtacgaagc
     2881 cgcgggcggg ctggtcgcgc gggacgcgtt ctacgtcgcg cggttcgacc acgagaagcg
     2941 ggagttcgac ttcgttctcc aggcggacgg cgaggacgtg tggcgcggcg agcggtatcc
     3001 gctcgcgacc ggcccgacga gccaggtcgt cgtgacaggc gagacctatg tggtccgctc
     3061 tccggaggac cccgcgcagc ggcgcggcat gaccttcggc gaggcgaccc gggtgtccgg
     3121 ctcggcggcg cacgtgccgc tgaagagccg cggccgtctg atcggtgtgc tctcgagcca
     3181 gtcgtactcg tcggacgcct tcgacgacga ggacatcgtg gtgctccagt cgctggcgaa
     3241 cctcgtggcg acggcgttcg agaacgccga acacatgcag cagatgcgcg agctgtacct
     3301 cgcgtccgtg aaggcgctgg ctgccgcggt ggacgcgcgc gacccgtaca cgcgcagcca
     3361 ctcggcgcgc gtcgcggcgc tggcgcgcac cgtcgcggag gagctgcgcc tcggtcccga
     3421 cgagctgcgc cgcgtccagc tcggcgcgct gctgcacgac atcggcaaga tcggcatccc
     3481 ggacgcgatc ctgaacaagc ccggtccgct ctccgccgac gaatgggtgc tcatgcgcac
     3541 gcatccggcg gtcggcgcct cgatcctcgc cgccgtcgag ccgctgcgcg acctcgtgcc
     3601 catcgtgcgc acgcatcacg agcgctacga cggcaacggc tatcccgagc acctcgccgg
     3661 cgacgagatc ccgatcgagt cgcacatcgt cgccgcggcc gacgccttcg aggtcatcgt
     3721 gagccggcgc gcgtacaagc aggcgcagac ggtggagttc gcctgcgccg agctgctgcg
     3781 ctgtcgcgcg acgcagttcc atcccgacgt cgtcgatgcg ttcctgcgcg tgatcgagcg
     3841 cgatcgccag cagggcgcga actacctccg tcgtgtcgcg ggcatcgagc acgaggagat
     3901 cgagaacgtc cccggtcccg gtggggtcct cgagcagttc gcggcgaccg cacacgcgca
     3961 cggccgccag ctcgcgatcc tgcagcggct cgcctctgag atcagcgcgg tcctcgacat
     4021 cgacgagctc gcgggcaggc tgctgcgcat cgtgtgcgag tccatgggat acgacagcgg
     4081 cttcctcctc acgctcgacg atcgacacac cggcctcgtc gtccgcgccg cggtcggtcc
     4141 gtcggagccg tacgtcggcc aggtgctccc ggccggcacc agcatctcgt ggtgggtcgt
     4201 cgagcacggc ctcccgcagc tcgtcgtcga cgtgcagaag gacgagcgtt attacggacc
     4261 gcccgacatc aacacggtgc tctgcgtccc cctgcagctc ggcgacgagc gcgtcggcgt
     4321 gctcggcatc gagagccggc gcgcgcaggc gttcggccgc gacgaccagg agctcctcac
     4381 ggccgtatcg caccaggtcg ccgccgcgct gcgcgtcgcc cggctgcatc agcaggcgaa
     4441 gcacgcggcc gcgacggatc cgctcacgag cctgccgaac cggcggacgt tcttcgagca
     4501 gctgcgccgc gagctctccg gcgatccggc ccaggccgtg agcatcgcgg tcctggacgt
     4561 gaacggcctg aaggagcgga acgacgagtt cggtcacgtc gccggcgacg aggccctcgt
     4621 gcgcatcggg gagatcctga cggccggcgt gcgcgacggc gacctggtcg cgcgcatcgg
     4681 cggcgacgag ttcgcgatcc tcttccccgc gacgacgcag ctcgccgcgg agcgcgtcat
     4741 gcgccgcctc gccgactgcg ttcgcgacgg caccgtcggc gacggccttc gtcttccgac
     4801 catcgcctgg ggtgtcgccg acgccgcgac ccgcacgatg acggtcgacg agctcgtcga
     4861 cgccgcggac cgcgccatgt accggcacaa gcaaaaggcg aggcgcgcca ccacggcgta
     4921 gggccgagag ccgggagcct cagccgcgcg tggcgtcccg ctcgtacacg acgcgcaggt
     4981 acatgacgtg gaggatcggg acctcgtacc actgcgagcc cacgacgcgg tagccctcgt
     5041 tcgcgaggcg ctggccgtcg cgctcggcct gcagcagcac acgctcctcg ggcgtgaacg
     5101 cgggctcgcc gccaaccgtg cgggcgaacg ctgtcagcag aggattggcg agggcgaaga
     5161 ggacgttgcc cttgccgtag atccgtacca cgcgagtgag cgtgctcgta cggtcgagac
     5221 tacgcctgcg cgaacgttac aatcggtggg ccggtcacga ccgggagaac accagacgcg
     5281 gcaccgacag agaaggcgcg tgacccgctg aaagcgcgcc gcggcaagca cgtcagggaa
     5341 gtcgcccgcg agccggatcg ctggtcccgt tagaggcccg aatgaggcgc gtcaacgctg
     5401 acgcgcgaag cctgggtggt acgacggcgc tgttcgtccc gggacgaacg gcgctttttc
     5461 tttgtccgaa acgcgaagcg tcccagcacg taacagaagg aggcgaccga catggcgaat
     5521 gcaacgacac cgcccgcggc ggtgctcgac ttcagcgaga agaacttccg gccgtcggtc
     5581 gtcgaaacgg agctctacga ctggtgggag cactcgggct tcttcacgcc gcccgccgag
     5641 ccgtcacccg gcgagaagac cttcgtgatg atgctgccgc tgccgaacgt caccggcgac
     5701 ctgcacatgg ggcacgcgct cggcttcggc ggctacgagg acctcatggc gcgctggcac
     5761 cgcatgaagg gcgaggccac gctctacctg ccgggtaccg accacgccgg gatcatcgcg
     5821 cagatcgtcg tggagaagca gctcgccaag gaggcgaatc cgaaggcgct cccgcgcgat
     5881 cagttcctcg acgagatgtg gaagtacatg cgccactacc agccgcgcat ctacaagcag
     5941 ttgcgcatgc tcgggtgctc cctcgactgg acgcgcgtcc acttcccgat ggatccggac
     6001 atgcagcgcc gcgtgcggat ccacttcatc cgtctgtaca agaacgggca cctgtatcgc
     6061 gacaaccgca tcgtgcactg gtgcctcacg tgccagacga cgtactccga tctcgagacg
     6121 ctgcacgtcg agcgcaccga ccagctgtcg tacgtgcgct atccctgggc cgacgcgatg
     6181 cccgcgggaa cgcccgacct cgtcatcgcg acgacgcggc cggagacgat cgtcgccgat
     6241 acggcgatcg cggtgcaccc ggacgacgac cgctggaagg cgtacatcgg gaaggacgtg
     6301 ctcgttccgc tggtcgagcg ccgggtgaag atcgtcggcg acgaggccgt cgatccggcg
     6361 ttcggaacgg gtgcgctcaa ggtcacgccg ggccacgacg cgaccgactt cgacatcggt
     6421 cagcgccacg gcctgcaggt catcagcgcg atcgacaagc gcggcacgat gatgccgtcg
     6481 gccggcatcc ttgccggcgc gcaccgcgac gccgcccgcg cgaaggcgat agaggtcctg
     6541 aagcaaaccg ggctcctgca gaagcaggag cccatcacac acgcgatcgc gatccacgac
     6601 cgctgctcga ccgtcgacga gccgctcgtg ctgaagcagt ggtggatgcg gatgaagccg
     6661 ctcgcgaagc ccgccatcga cgcggtgcga gacggccgca tccagatcat cccgaggtac
     6721 caggagaaga tctacttcga gtggatggag aacatccgcg actggtccat cgcgcgccag
     6781 atctactggg gccactccat cccggcctgg tactgccagg actgcgacga gatcgtcgtt
     6841 cccgatgagg acgcgcccga tccgacgcag tgcacgaagt gccgcagcga cgacctccgg
     6901 cgcgatcccg acgtgctcga cacgtggttc tcgtctgccc tctggccgca cgtgacgctc
     6961 gggtggcccg agaagacgaa ggatctcgag cgcttctacc cgggcacgat gctcgagacc
     7021 ggctgggaca tcctgttctt ctgggtggcc cgcatgatca tgatgggctt gaagttcacc
     7081 ggcgaggtcc cgttcc
//
LOCUS       DASTZR010000192         6730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_973838, whole genome shotgun sequence.
ACCESSION   DASTZR010000192 DASTZR010000000
VERSION     DASTZR010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6730
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973838"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VFV20_04255"
     CDS             complement(<1..331)
                     /locus_tag="VFV20_04255"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEU5287592.1"
                     /translation="MPLGAHRFGPHLDRERSAFEGGPLSEASSRGRDGAPRFVVVTGV
                     SGAGKTQAVKVFEDLGYYILDNLPPALMVSALDVCRRGGHERVAFVIDARSGPLFADA
                     SAALDELA"
     gene            complement(259..642)
                     /locus_tag="VFV20_04260"
     CDS             complement(259..642)
                     /locus_tag="VFV20_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEU5287593.1"
                     /translation="MGFLLRAVVTAIAIAITAWIMDIVLPGSIRWGLVDYGLGEVGRY
                     LSLLLAAAVLGIVNAFVRPILLMISMPLTCATLGLFVFVINALMLLLVSAVPQLGLSV
                     DGFLSALIGSVLISIVSGVLSKVVH"
     gene            complement(662..1483)
                     /locus_tag="VFV20_04265"
     CDS             complement(662..1483)
                     /locus_tag="VFV20_04265"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5287594.1"
                     /translation="MDELRAALALARKDIRNMSRYRFVVASQIFTPLYQGVLPGLLFG
                     ASFAVGGKVVGLDKMIGTDDLTGFIFLGASMSGLVATAFWAMGMSFRNEMEMGTLEPT
                     WLAPTAKETLVLGRALGGLFWFLFSQTLIFAIGIAFFGLRLRPEIVYAIPAILVATVA
                     LIGVAHLLAGIVLSIRDANLFIDCTSFLFATASGAAFPVSLLPGVLQPVAYLLPTTYA
                     MDIVRQHALGTRPLIDPALEYVGLLVTTALMFPLGRWAFDRAERRMRVRGTLAQY"
     gene            complement(1483..2331)
                     /locus_tag="VFV20_04270"
     CDS             complement(1483..2331)
                     /locus_tag="VFV20_04270"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5287595.1"
                     /translation="MTCSLAVADARAFLAAGHREFRLLRRYPAAFVGQIFWPVLLPSV
                     WVLMGQAYSGGGDAQAMDAFASRSGTTGITLFIFVGYAMYMWLSTLLWGPGTSLRRDQ
                     MTGTLEAVFLTPASRLVALFGPTLVNAVWTLFHLVIMGIAVWLLFGVVLPLEGVLLTF
                     LVVLAGLPAMYAMGSFFSAGVLRFGEVGPAVQLARGTLSLLCGITFPLVMLPAWAQTT
                     AAVMPPTYIVETMRNVLLKGAGPLDVARVAVVLLALAVAFGILAIVVFTALERSARRT
                     GMLGRY"
     gene            complement(2328..3341)
                     /locus_tag="VFV20_04275"
     CDS             complement(2328..3341)
                     /locus_tag="VFV20_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018221340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5287596.1"
                     /translation="MTRGAAVEARGLVKEFEKGRRTIWQRLLRKPDRRTIFRAVDGVD
                     LTVEQGEIFGLLGPNGAGKTTTMKMLATLLEPTAGTISVLGIDVAKEPRKVRARLGAM
                     LSGERSLYWKLTGRENLEYFAALYHVPPHETKERIERSLDAVKLRDRADDYVERYSTG
                     MRQRLALARALLPDPPLVILDEPTVGLDPQSARDLRERVRDLARQGRTVLLTTHYMEE
                     ADQLCDRIAIIDHGQIVALDTPAELKRRIRAEDVVRLEIGGSGDDAALVARLGSGASI
                     ARTERQNGTLAVTAHCASARDFVPAAFDAARSFGATIQHVEVVPVSLEDVFISLTGRA
                     LRE"
     gene            complement(3338..3748)
                     /locus_tag="VFV20_04280"
     CDS             complement(3338..3748)
                     /locus_tag="VFV20_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287597.1"
                     /translation="MPSEEGKQVLRLLAEGKIDSEQAYRLLKALGDIDETPPARHSPP
                     GPPPPRPGPAAPGARGRILRIRVTEGGEQKVNVAIPLAIARIGKMKLGSSGLVRGHLS
                     KFGIDLDELLRSIDFPGRVVDVTDDDDRVEIFVE"
     gene            complement(3749..5152)
                     /locus_tag="VFV20_04285"
     CDS             complement(3749..5152)
                     /locus_tag="VFV20_04285"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEU5287598.1"
                     /translation="MSEERRDEQPDWSRFDPHEAEREARRAERDARDAERDQARAARE
                     QEHAARAEARDAERMARDAERMARHADRAERRENRRGRHIWITGQGLHLGGIDVEGMG
                     FGDLAKGFMKDFAGDMAGGTYDETVTGEFTFERMPNLHVRNISGETTVKTGEPGKISV
                     VATKHVSAGSEDRAKRLLQNLEIRMEQHGDDLHVEPHLYEQERGWVDLFRGKRVRVDL
                     EITVPVQCGIDAHTVSGDMSVTGTRGPLEMQSVSGDVTLADAQGPLRLKSVSGDVNVS
                     RYVGHIEGNTVSGDVAFEAVRVRSIQLHTVSGDIELAGMLEPAREHRFRTISGDVHVK
                     LVEPDLTVEFRTASGDIESELPGRVTRQGRKEYSVALGDGRGHALVKTVSGDLRIDGS
                     TVDVPGEPSTEEPRGAGRAPVVPEAADEAEADVEKTEPMETGPSEQVRSVLERLAKGE
                     LNVDDAAAAIDAARRGL"
     gene            complement(5149..5532)
                     /locus_tag="VFV20_04290"
     CDS             complement(5149..5532)
                     /locus_tag="VFV20_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HEU5287599.1"
                     /translation="MANPVVARCPVCSEQLRVVRLECESCGTRLEGSFSLGRFHALAP
                     EQLEFLEVFIRARGNFKDVERELGISYPTVRSRLDAVIRALGFPSQAEPDREAERRKE
                     ILRELAEGRIAPDDAATLLETETTR"
     gene            complement(5625..>6730)
                     /locus_tag="VFV20_04295"
     CDS             complement(5625..>6730)
                     /locus_tag="VFV20_04295"
                     /inference="COORDINATES: protein motif:HMM:NF020048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU5287600.1"
                     /translation="EVHAYKGDDFDVLAASLSENDAVVQLFRVRDGTVVGRDHFAMEG
                     SEGASPGEVVASFLRLHYAAAGTLPPEVVTPSPLPDGEALETFLAERRGGPARIHVPQ
                     RGRKRHLAELAERNAADALAQERVRWLADRGKTDTALKELQDALGLEGPPKRIECYDV
                     SHVQGTSVVSSMVVFEDGRPAKSQYRRFRARIGDRNDDFANMRETLRRRFSRSAQGDE
                     TTGTAWALPDLVILDGGKGQLSAGLDALADAGRLQIPIVALAKEREELFLPGRPDPVV
                     LPRTSQGLYLVQRIRDEAHRFAVTYHQKVRSKRAVRSLLDDVAGVGPAKKRALLRKFG
                     SVRGMREAGLDDLAAVAGVGSALAKRIKDALDS"
BASE COUNT         1051 a         2358 c         2361 g          960 t
ORIGIN      
        1 ccgcgagctc atcgagggcg gcgctcgcgt cggcgaagag cggaccgctg cgcgcgtcga
       61 tcacgaaggc gacgcgctcg tgcccgcccc gtcggcagac gtcgagcgcg gacaccatga
      121 gcgcgggcgg caggttgtcg aggatgtaat agccgaggtc ctcgaacacc ttgaccgcct
      181 gcgtcttccc cgcccctgag acgccggtga cgacgacgaa gcggggcgcc ccatcacggc
      241 cgcgggacga ggcctcgctc agtggaccac cttcgaaagc actccgctca cgatcgagat
      301 gaggaccgaa ccgatgagcg ccgagaggaa tccgtcgacg ctgaggccca gctgcggcac
      361 cgcggagacg aggagcagca tgagcgcatt gatgacgaag acgaagaggc cgagcgtggc
      421 gcacgtgagc ggcatcgaga tcatcaggag gatcggcctg acgaacgcgt tcacgatgcc
      481 gagcacggcg gccgcgagca gcagcgagag gtagcgaccg acctcaccga ggccatagtc
      541 gacgagcccc cagcgaatgc tgccgggaag gacaatgtcc atgatccagg cggtgatggc
      601 gatggcgatc gcggtgacga ccgcccgcag caggaagccc atcgcgtaca gactagaccg
      661 tttagtactg ggcgagcgtt ccgcgtacgc gcatgcggcg ctcggcgcga tcgaaggccc
      721 agcggccgag cgggaacatc agcgccgtgg tcacgaggag gcccacgtac tcgagcgccg
      781 ggtcgatgag cggccgcgtg cccagcgcgt gctggcgaac gatgtccatc gcgtaggtcg
      841 tgggcagcag gtaggcgacg ggctgcagca cgcccggcag caggctgacc gggaaggccg
      901 cgccggaggc cgtcgcgaag aggaagctcg tgcagtcgat gaacaggttc gcgtcgcgga
      961 tcgacagcac gatccccgcg agcaggtgcg ccacgccgat cagggcgacc gtcgccacga
     1021 ggatcgcggg tatcgcgtag acgatctccg gccggaggcg caggccgaag aacgcgatgc
     1081 cgatggcgaa gatcagggtc tggctgaaga ggaaccagaa gagcccgccg agcgcgcgcc
     1141 cgaggaccag cgtctccttc gcggtgggcg cgagccaggt gggctcgagc gtgcccatct
     1201 ccatctcgtt ccggaagctc atgcccatcg cccagaatgc cgtggccacg agcccgctca
     1261 tgctcgcgcc caggaagatg aagccggtga ggtcgtccgt gccgatcatc ttgtcgaggc
     1321 cgaccacctt gccgccgacg gcgaacgacg cgccgaagag gagacccgga aggacaccct
     1381 gatagagcgg cgtgaagatc tgagacgcga cgacgaaccg gtagcgggac atgttccgga
     1441 tgtccttgcg cgcgagcgcg agggcggccc tgagctcgtc catcaatatc tcccgagcat
     1501 cccggtacgc cgcgccgacc gctcgagcgc cgtgaacacg acgatggcga ggatgccgaa
     1561 cgcgaccgcg agcgcgagga gcaccaccgc cacgcgcgcg acgtcgagcg gcccggctcc
     1621 tttgagcagc acgttgcgca tcgtctcgac gatgtacgtc ggcggcatca ccgcggcggt
     1681 ggtctgcgcc catgccggca gcatcacgag cgggaaggtg atgccgcaga ggagcgacag
     1741 cgtcccgcgc gcgagctgga ccgccgggcc gacctcgccg aagcggagga cgcccgcgct
     1801 gaagaaggag cccatggcgt acatcgccgg cagtcccgcg agcacgacga ggaacgtgag
     1861 cagcacgccc tcgagcggca gcaccacgcc gaagagcagc cacaccgcga tacccatgat
     1921 cacgaggtgg aacagcgtcc acaccgcgtt gaccagcgtg ggaccgaaga gcgcgacgag
     1981 ccgggaggcc ggcgtgagga acaccgcctc gagcgtgccg gtcatctggt cgcgtcgcag
     2041 cgacgtgccg ggaccccaga gcagcgtgga cagccacatg tacatcgcgt agccgacgaa
     2101 gatgaagagc gtgatgcccg tggtgccaga gcgcgacgcg aaggcgtcca tggcctgcgc
     2161 gtcgccgccg cccgagtacg cctgccccat gagtacccac accgagggca ggagcacggg
     2221 ccagaagatc tgcccgacga acgccgcggg gtagcggcgc agcagccgga actcgcgatg
     2281 gcccgcggcg aggaacgcgc gcgcgtccgc gacggcgagc gagcaggtca ttcgcgcagc
     2341 gcgcgtccgg tgagcgagat gaagacatcc tcgaggctca ccggaacgac ctccacgtgc
     2401 tggatcgtcg cgccgaagct gcgcgcggcg tcgaacgccg ccggtacgaa gtcgcgcgcc
     2461 gacgcgcagt gcgcggtcac cgcgagcgtc ccgttctgcc gctcggtgcg cgcgatcgat
     2521 gcgcccgagc cgaggcgcgc cacgagcgcg gcatcgtcgc cggagccacc gatctcgagg
     2581 cgcacgacat cttccgcgcg gatgcgccgc ttcagctcgg cgggcgtgtc gagcgccacg
     2641 atctggccat ggtcgatgat cgcgatgcgg tcgcagagct gatcggcttc ctccatgtag
     2701 tgcgtggtga ggagcacggt gcggccctga cgcgcaaggt cgcgcacgcg ctcgcggagg
     2761 tcgcgcgccg actgcgggtc gagtccgacc gtcggctcgt cgaggatgac gagcggcggg
     2821 tccggcagca gcgcgcgcgc gagcgcgaga cgctggcgca tgccggtgga gtagcgctcc
     2881 acgtagtcgt ccgcgcggtc gcgcagcttc accgcgtcga ggctgcgctc gatgcgctcc
     2941 ttcgtctcgt gcggcggcac gtggtacagc gcggcgaagt actcgaggtt ctcgcggccc
     3001 gtgagcttcc agtacaggct gcgctcgccg gagagcatcg cgccaaggcg agcgcgcacc
     3061 ttgcggggct ccttcgcgac gtcgatgccg aggacgctga tggtgcccgc ggtcggttcg
     3121 agcagcgtcg cgagcatctt catcgtggtc gtcttgccgg ccccgttcgg accgaggagc
     3181 ccgaagatct cgccctgctc caccgtgaga tcgacaccgt cgacggcccg gaagatcgtc
     3241 cgccgatccg gttttcgcag caggcgctgc cagatcgtac ggcggccctt ctcgaactcc
     3301 ttgaccagcc cgcgcgcctc gacggcggcg ccgcgtgtca ttcgacgaag atctctaccc
     3361 gatcgtcgtc gtcggtgaca tcgacgacac ggcccgggaa gtcgatggag cggagcagct
     3421 cgtccagatc gatcccgaac ttcgacagat gcccgcgcac gagcccgctc gagcccagct
     3481 tcatcttccc gatgcgcgcg atcgcgagcg ggatcgcgac gttgaccttc tgctcgccgc
     3541 cttcggtcac gcgaatgcgc aggatgcggc ctcgcgcccc cggagccgcc gggcctggtc
     3601 gcggcggcgg gggtcccggc ggcgagtgtc gcgcgggcgg cgtctcgtcg atgtcaccca
     3661 gggccttcaa cagacggtag gcctgctcgc tgtcgatctt gccttcggcg agcagacgca
     3721 ggacctgctt cccttcctcg ctcggcatct acagaccgcg gcgcgccgcg tcgatcgccg
     3781 ccgcggcatc gtcgacgttc agctcgccct tcgcgagccg ctcgagaacg ctccgcacct
     3841 gctcgctcgg accggtctcc atcggctccg tcttctcgac gtccgcctcc gcctcatccg
     3901 cggcctccgg cacgactggc gcgcggcccg cgccgcgcgg ctcctcggtg gacggctcgc
     3961 cggggacgtc gacggtgctg ccgtcgatcc ggaggtcgcc gctcaccgtc ttcaccagcg
     4021 cgtggccgcg accgtcgccc agcgccacgg agtactcctt gcgaccctgc cgcgttacgc
     4081 gacccggcag ctcggattcg atgtcaccgc tcgcggtgcg gaactccacc gtcaggtccg
     4141 gttcgacgag cttcacgtgg acgtcgccgc tgatcgtgcg gaaccggtgc tcgcgggcgg
     4201 gctcgagcat gccggcgagc tcgatgtcgc cgctcaccgt gtggagctgg atcgagcgga
     4261 cgcggacggc ctcgaaggcg acgtcgccgc tcacggtgtt gccctcgatg tgaccgacat
     4321 agcggctcac gttcacgtcg ccgctcacgc tcttgagccg cagcggcccc tgcgcgtcgg
     4381 cgagcgtcac gtcgcccgag acgctctgca tttcgagcgg accgcgcgtg cccgtcaccg
     4441 acatgtcgcc gctgacggtg tgcgcgtcga tcccgcactg gacgggcacg gtgatctcga
     4501 ggtcgacgcg gacccgcttg ccgcggaaca ggtcgaccca gccgcgctcc tgctcgtaca
     4561 ggtgcggctc cacgtgcaga tcgtctccgt gctgctccat ccggatctcg aggttctgga
     4621 gcaggcgctt cgcgcggtct tcgctgccgg cgctcacgtg cttggtcgcg acgacgctga
     4681 tcttccccgg ctcaccggtc ttgaccgtcg tctcgccgga gatgttgcgg acgtggaggt
     4741 tcggcatccg ttcgaacgtg aactcgccgg tcaccgtctc gtcataggtc ccgcccgcca
     4801 tgtcccccgc gaagtccttc atgaagccct tggcgagatc gccgaagccc atgccctcga
     4861 cgtcgatgcc gccgaggtgg agcccctgac cggtgatcca gatgtgccgt ccgcgccggt
     4921 tctcgcggcg ctccgcgcgg tcggcgtggc gcgccatgcg ctccgcgtca cgcgccatcc
     4981 gttcggcgtc gcgcgcctcg gcgcgcgcgg cgtgctcctg ctcacgggcg gcccgcgcct
     5041 gatctcgctc ggcatcgcgc gcgtcacgct cggcgcgacg cgcctcgcgc tcagcctcgt
     5101 ggggatcgaa ccgcgaccag tcgggctgtt cgtcgcgacg ctcttcgctc atcgggtcgt
     5161 ctccgtttcc agcagggtgg cggcatcgtc cggcgcgatg cggccctcgg cgagctcccg
     5221 aaggatctcc ttgcgtcgct cggcctcacg gtccggctcg gcctgcgagg ggaatccgag
     5281 cgcgcggatg accgcgtcga gacgggagcg caccgtcggg tacgagatcc cgagctcgcg
     5341 ctcgacgtcc ttgaagttgc cgcgagctcg gatgaagacc tcgaggaact ccagctgttc
     5401 gggcgcgagc gcgtggaagc gaccgagcga gaacgagccc tccagacggg tcccgcagct
     5461 ctcgcactcc aggcgcacga cgcgcagctg ctcagagcag acggggcacc gggccaccac
     5521 cggattcgcc atgactcggt tatgcgcggc gacctaaatg aaagtcaagc gaaagtgagg
     5581 gttgtgtagc cgagatatcg aaacggtgag gtcgagaggg cgagctacga atcgagcgcg
     5641 tccttgatgc gcttcgcgag cgcgctgccg acaccggcca ccgcggcgag atcgtcgagg
     5701 cctgcctcgc gcatcccgcg caccgagccg aacttccgca ggagggcgcg cttcttcgca
     5761 gggccaacgc cggcgacgtc gtcgagcagc gagcggaccg cccgcttcga gcgcaccttc
     5821 tggtggtacg tcaccgcgaa gcggtgggcc tcgtcgcgga tgcgctgcac caggtagagg
     5881 ccctgcgagg tgcgcgggag cacgaccgga tccggccggc cgggcaggaa gagctcctcg
     5941 cgctccttgg cgagcgcgac gatcgggatc tgcaaccggc cggcgtcggc gagcgcgtcg
     6001 aggccggcgg agagctgtcc cttgccgccg tccaggatca cgaggtcggg cagcgcccag
     6061 gccgtgccgg tcgtctcgtc accctgagcg ctccgcgaga atcggcgccg cagcgtctcg
     6121 cgcatgttcg cgaagtcgtc gttgcggtcg ccgatgcgcg cgcggaagcg ccggtactgc
     6181 gacttcgccg ggcggccgtc ctcgaacacg accatcgacg acacgacgct cgtgccctgc
     6241 acgtggctca cgtcgtagca ctcgatccgc ttcggcgggc cctcgagccc gagcgcgtcc
     6301 tgcagctcct tgagcgccgt gtcggtcttc ccgcgatccg cgagccagcg cacccgctcc
     6361 tgcgcgagcg cgtcggcggc gttgcgctcc gccagctcgg cgagatggcg cttccgtccg
     6421 cgctgcggga catggatgcg cgcggggccg ccgcgtcgct ccgcgaggaa ggtctcgagg
     6481 gcctcgccgt ccggcagcgg cgacggcgtg acgacctcgg gcggaagcgt gccggccgcc
     6541 gcgtaatgca gccgcaggaa ggacgcgacg acctcaccgg ggctcgcgcc ctccgagccc
     6601 tccatggcga agtgatccct gccgaccacg gtcccgtcgc ggacgcggaa gagctgcacg
     6661 acggcgtcgt tctcggagag cgacgcggcg agcacgtcga agtcgtcgcc cttgtatgcg
     6721 tggacctcct
//
LOCUS       DASTZR010000193         3372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_662733, whole genome shotgun sequence.
ACCESSION   DASTZR010000193 DASTZR010000000
VERSION     DASTZR010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662733"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1502
                     /locus_tag="VFV20_04300"
     CDS             <1..1502
                     /locus_tag="VFV20_04300"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEU5287601.1"
                     /translation="AEGLLMETDTEVSGDLQLTGLQKHFDGSYPILFNRVKGYPHVRA
                     ITNFFANMDIVDRMFGWEDRTERTRKLAHALTHPIPNEIVKQADAPVMEEVITDDLDV
                     NKYIFPIRHTHLESEITIGSGNSVVVGDKFWGGSHIGYNRMNFRWGNVGTFQISPGSH
                     MWQVMTKYYKDEPVPLTVNFGLPAAATLLAGGGFDYVVLPRGGDELGAAGAVQGSPIR
                     LVKAATVDAYACADAEYSLEGYLHPRDKRYETAEAEKADIQGRFHFHPEWAGYMGKAY
                     KAPTFHVTAITMRKRSERPIIYPMGVHMYDCNNIDTTVREAAFYELCERIQPGLINDV
                     NIPFPMTDWAGAILQVKKRLKTDDGFVRNMLAAAMSCSSGLRLCIAVDSDVDIYSMDD
                     IIWSLTTRVNPRDDVWSPTPGGAGQTFIPEQRLTAGNAQWTAMNTRFEGGMAIDATVP
                     YGYENDFMRPVYPIDRVDPAGWFDNDQIAKGKALMKTQSWAEVLAKSGR"
     gene            1515..2693
                     /locus_tag="VFV20_04305"
     CDS             1515..2693
                     /locus_tag="VFV20_04305"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU5287602.1"
                     /translation="MDGLRVVELVEADIWGAAAVAHCGRTLADLGADVVKAEPPAGDP
                     VRRVPPFIGERADADRGLMWIALNANKRGVVLPRGDAVARDAVVRDADVVLQPRLVVD
                     PRAHPGRVVVTITPFGATGPLADVPASDLEVTAASGSLWLAGEPGGPPVRTTLPQSPF
                     WTGMYATAGALLAVVARRRTGRGQHVDVSGQQSMVTVHPPAAVFADVAREEHVRLGPN
                     LLGRSIVGARFRNVWPCADGHVSFAIQGGPIGRHTGRMLSAWMAERGCEARRFRSIEW
                     DTFDNRTLSQADVDALEAEIGAFLATLTKREFFDGVVARNMLGYPVATAADIYANAQL
                     LARAFWQDVAIDGETLTFPGGFALFDGERPAVRRAPPRLGEHQREVLPDLAPSGPKTP
                     "
     gene            2690..>3372
                     /locus_tag="VFV20_04310"
     CDS             2690..>3372
                     /locus_tag="VFV20_04310"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU5287603.1"
                     /translation="MTAPGPDPTSDPRAPLHGVRVVELGWAAAGPLVGTYLASHGADV
                     IHLESATALDPFRSTYPPFKDNQPGPDRAAMFSFYNAGKRGVTLDLKHPAAVDLALRL
                     IATADVVIESFPAGTLARRGLGIDAMRAVKPDLIVFSSCNQGQTGPQAQHPGYGSQLT
                     ALAGFTHLLGDPARTPVILYGPYIDYIAVGYGVVAILAALDRRARTGRGCVIDLSQYE
                     AGLQFVAPAL"
BASE COUNT          518 a         1220 c         1145 g          489 t
ORIGIN      
        1 gcgccgaagg cctgctcatg gagaccgaca ccgaggtgtc gggtgacctt cagctcaccg
       61 ggctgcagaa gcacttcgac ggcagctacc cgatcctgtt caaccgggtg aagggctatc
      121 cccacgtgcg cgcgatcacg aacttcttcg cgaacatgga catcgtcgac cgcatgttcg
      181 gctgggagga ccgcaccgag cgcacgcgga agctcgcgca cgcgctcacg cacccgatcc
      241 cgaacgagat cgtgaagcag gcggacgcgc ccgtgatgga ggaggtcatc accgatgacc
      301 tcgacgtgaa caagtacatc ttcccgatcc gccacacgca cctcgagagc gagatcacca
      361 tcgggtcggg caacagcgtg gtcgtcggcg acaagttctg gggcggcagc cacatcggct
      421 acaaccgcat gaacttccgc tggggcaacg tcggcacctt ccagatctcg cccggctcgc
      481 acatgtggca ggtcatgacg aagtactaca aggacgagcc ggtgccgctc acggtgaact
      541 tcggcctgcc ggccgcggcc accctcctcg ctggtggtgg gttcgattac gtcgtgctgc
      601 cgcgcggcgg cgacgagctc ggcgccgcgg gcgccgtgca gggatcgccg atccgcctcg
      661 tgaaggccgc gacggtggac gcgtacgcgt gcgccgacgc cgagtactcg ctcgagggct
      721 acctgcatcc gcgcgacaag cgctacgaga cggccgaggc tgagaaggcc gacatccagg
      781 gacggttcca cttccatccg gagtgggccg gctacatggg aaaggcctac aaggcgccga
      841 cgttccacgt gaccgcgatc acgatgagga agcgcagcga gcgcccgatc atctatccga
      901 tgggcgtgca catgtacgac tgcaacaaca tcgacacgac ggtgcgtgag gccgccttct
      961 acgagctctg cgagcgcatc cagcccggcc tcatcaacga cgtgaacatc ccgttcccga
     1021 tgaccgactg ggccggcgcg atcctgcagg tcaagaagcg cctcaagacc gacgacggat
     1081 tcgtgcgcaa catgctcgcc gccgcgatgt cgtgctcgtc cggcctgcgg ctctgcatcg
     1141 ccgtcgacag cgacgtcgac atctactcga tggacgacat catctggtcg ctcacgacgc
     1201 gcgtgaatcc gcgcgacgac gtgtggagcc ccacgcccgg cggcgcgggc cagaccttca
     1261 tcccggagca gcggctcacc gccgggaacg cgcagtggac ggcgatgaac acgcgcttcg
     1321 agggtggcat ggccatcgac gccacggtcc cctacggcta cgagaacgac ttcatgcgtc
     1381 cggtgtaccc gatcgacagg gtcgatccag cgggctggtt cgacaacgac cagatcgcga
     1441 aggggaaggc gctcatgaag acgcagtcgt gggccgaggt gctggcgaag agcggccgct
     1501 aaaggccgcg cgtgttggac ggccttcgcg tcgtcgagct cgtcgaagcc gacatctggg
     1561 gtgcggccgc ggtcgcgcac tgcggccgca ccctcgccga cctcggcgcg gacgtcgtga
     1621 aggccgagcc accggccggc gatccggtcc gtcgcgtgcc accgttcatc ggcgagcgcg
     1681 cggatgccga ccgcggtctc atgtggatcg cgctcaacgc gaacaagcgc ggcgtcgtcc
     1741 tgccccgcgg tgacgcggtg gctcgggacg cggtcgtacg cgacgccgac gtcgtgctgc
     1801 agccccggct ggtcgtcgat ccgcgcgcgc accccggccg ggtggtcgtc accatcacgc
     1861 cgttcggcgc caccggcccg ctcgcggatg tccccgcgtc ggatctggag gtcaccgcgg
     1921 cgagcggcag cctctggctc gcgggcgagc ccggcggccc gccggtgcgc acgacgctgc
     1981 cgcagtcgcc gttctggacc ggcatgtacg cgaccgccgg cgcgctcctc gccgtcgtcg
     2041 cgcgccggcg caccggccgc gggcagcacg tggacgtctc gggacagcag agcatggtca
     2101 cggtccaccc gccggccgcc gtgttcgccg acgtcgcgcg cgaagagcac gtgcgcctcg
     2161 gccccaatct cctgggccgc agcatcgtcg gcgcgcgctt ccggaacgtg tggccgtgcg
     2221 cggatggtca cgtgtcgttc gcgatccagg gcggtcccat cggacggcac accggccgga
     2281 tgctgtccgc gtggatggcg gagcgcggct gcgaggcgcg ccgcttccgt tccatcgagt
     2341 gggacacctt cgacaaccgc acgctgtcgc aggccgacgt agacgcgctg gaggccgaga
     2401 tcggcgcctt cctcgcgacg ctcacgaagc gcgagttctt cgacggcgtc gtcgcgcgca
     2461 acatgctcgg ctaccccgtc gcgaccgcgg ccgacatcta cgcgaacgcg cagctcctgg
     2521 cccgtgcgtt ctggcaggac gtggcgatcg acggcgagac gctgacgttc cccggcggct
     2581 tcgcgctgtt cgacggcgag cgccccgcgg tgcgccgcgc gccgccgcgc ctcggcgagc
     2641 accagcgcga ggtcctgccc gatctcgccc cgtcgggacc caagacaccg tgaccgcccc
     2701 aggcccggat ccaacgtcgg acccgcgcgc gccgctgcac ggcgtccgcg tcgtggagct
     2761 cgggtgggcc gcggccgggc cgctcgtcgg cacgtacctc gcctcgcacg gcgccgacgt
     2821 catccacctc gagtccgcga cggcgctgga ccccttccgc tcgacgtacc cgccgttcaa
     2881 ggacaaccag cccggtccgg accgcgccgc gatgttctcc ttctacaacg ccggcaagcg
     2941 cggggtgacc ctggacctga agcatccggc ggcggtcgat ctcgcgctgc gcctgatcgc
     3001 gaccgccgac gtggtgatcg agtcgttccc cgcgggtacg ctcgcgcgcc gcgggctcgg
     3061 catcgacgcg atgcgtgcgg tgaagcccga cctcatcgtg ttctcgtcat gcaaccaggg
     3121 tcagaccggt ccgcaggcgc agcatccggg atacggcagc cagctcacgg cgctcgcggg
     3181 cttcacgcac ctgctgggcg accccgcgcg cacgccggtc atcctctacg ggccgtatat
     3241 cgattacatc gccgtcgggt acggcgtcgt ggccatcctc gcggcgctcg accggcgcgc
     3301 gcgcaccggt cggggatgcg tcatcgacct ctcgcagtac gaggcgggtc tccagttcgt
     3361 cgcgccggcg ct
//
LOCUS       DASTZR010000194         2472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_924998, whole genome shotgun sequence.
ACCESSION   DASTZR010000194 DASTZR010000000
VERSION     DASTZR010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924998"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VFV20_04315"
     CDS             complement(<1..424)
                     /locus_tag="VFV20_04315"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5287604.1"
                     /translation="MAERVFVRGFGGDYGLKEERKRRLAAPRVIHTKDLPWGTGPNHW
                     NRDILRPDMEPFRTQTLDIHMEAEAPGNVSHKHGHQNEACFYILEGRGHEIHDGKRYE
                     WKAGDLVIVHNDSVHQHWNDDPEHPTRALIIKAKPLWLF"
     gene            complement(424..981)
                     /locus_tag="VFV20_04320"
     CDS             complement(424..981)
                     /locus_tag="VFV20_04320"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEU5287605.1"
                     /translation="MEAPDLPNAVGVSLDPRTTAVLVLDLSDVSCGQVPAAKESIAPV
                     RKLLDRAREHGARVIFSLGRAPQEVYPDLGRRESEPIVKSSADKFFQTDLEEQLKGIT
                     HAVVIGTAANGAVLYTAFGACARGMAVVVPEDGISSRQPIATWVARYQLLNQPGFVNA
                     QNTPLQAKAVTLSRTDLIAFVKASA"
     gene            complement(1004..1945)
                     /locus_tag="VFV20_04325"
     CDS             complement(1004..1945)
                     /locus_tag="VFV20_04325"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU5287606.1"
                     /translation="MRLPRRPIQWPNDARIAVMPTIVLETWPTEELGKPGSAQAQFRD
                     DFPKGSLFTTDIALITDRLYGERCGAYRMLDILEEAGIHTSWFLNGAGVEAFPDLAKE
                     IRSAGHELASENWRHEYVGRMTDEQQKADLEKTLAAFAKIGAKPTSYISPGERPTDYT
                     LELIAEKGYDCYMGSLNDDLPYTLRTKKGDLVMFSYGIWMTDHQMASRTRKNPRDILQ
                     WWKDQFDQLYEEGEKGRPGLMTMGLHPFITGRPFRAKVLREFFAYATSKPKVWFPRSD
                     EATKWYLDNYREQYVETWPHYGTGMPRPKEKVAAGAR"
     gene            complement(2009..>2472)
                     /locus_tag="VFV20_04330"
     CDS             complement(2009..>2472)
                     /locus_tag="VFV20_04330"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEU5287607.1"
                     /translation="NIVQEGGPRDIYTSPSSGFVADFVGIGTFVRAEIDRGGVRALGG
                     RVSCAPPDDLPVGSQAHLVLRPENVYVDVEPKGVENEFEGTLRVAAFLGDHLDCLVDV
                     AGELVRARAHPAQRLVRDQKVWIRMPAEQCVLIRDDGWQPRPLARSYAEED"
BASE COUNT          352 a          829 c          843 g          448 t
ORIGIN      
        1 tgaagagcca gaggggcttc gccttgatga tcagcgcgcg tgtcggatgc tcggggtcgt
       61 cgttccagtg ctggtgcacg gaatcgttgt ggacgatgac gaggtcgccg gccttccact
      121 cgtagcgctt gccgtcgtgg atctcgtgtc ctctgccctc gaggatgtag aagcaggcct
      181 cgttctgatg gccgtgcttg tgggacacgt tgcccggcgc ctccgcctcc atgtggatgt
      241 ccagggtctg ggtgcggaac ggctccatgt ccggccggag gatgtcgcga ttccagtggt
      301 tgggaccggt gccccacggc aggtccttgg tgtgtatgac acgcggcgcc gccaggcgcc
      361 gcttccgctc ctccttcagg ccgtagtcgc cgccgaagcc tcggacgaag acacgctccg
      421 ccatcaggcg ctcgccttca cgaaggcgat gaggtcggtg cgcgacagcg tcacggcctt
      481 cgcctggagc ggtgtgttct gcgcgttcac gaagcctggc tggttcagca gctggtagcg
      541 tgcgacccac gtcgcgatcg gctgccgcga cgagatgccg tcttcgggga ccacgacggc
      601 catcccgcgc gcgcacgcgc cgaacgcggt gtagagcacg gcgccgttcg cggccgtgcc
      661 gatcacgacc gcgtgcgtga tccccttgag ctgctcctcg agatcggtct ggaagaactt
      721 gtcggcgctc gacttcacga tcggctcgct ctccctgcgt ccgaggtcgg gatacacctc
      781 ctgcggcgca cggccgagcg agaagatcac gcgcgcgccg tgctcgcgcg cgcgatcgag
      841 gagcttccgc accggcgcga tgctctcctt cgccgcgggc acctgcccgc acgacacgtc
      901 ggagagatcg agcacgagga ccgcggtcgt tcgcgggtcg agcgacacgc ccaccgcgtt
      961 cggcagatcg ggtgcttcca tcgttcccat gagtttcctc ccgctaccgc gcgcccgccg
     1021 cgaccttctc cttgggacgc ggcatgcccg tgccgtagtg cggccacgtc tcgacgtact
     1081 gctcgcggta gttgtcgaga taccacttgg tggcctcgtc cgaccgcggg aaccacacct
     1141 tcggcttgga ggtcgcgtac gcgaagaact cgcggaggac cttggcacgg aacgggcggc
     1201 ccgtgatgaa cgggtgcagg cccatggtca tgaggccggg ccggcccttc tcaccctctt
     1261 cgtagagctg gtcgaactgg tccttccacc actgcaggat gtcgcgcggg ttcttgcgcg
     1321 tcctgctcgc catctggtgg tcggtcatcc agatcccgta cgagaacatg accaggtcgc
     1381 ccttcttcgt gcggagcgtg tacggcaggt cgtcgttgag cgaccccatg tagcagtcgt
     1441 agcccttctc cgcgatgagc tcgagcgtgt agtccgtcgg acgctcgccg ggcgagatgt
     1501 agctcgtcgg cttcgcaccg atcttcgcga acgccgcgag cgtcttctcg agatcggcct
     1561 tctgctgctc gtcggtcatc cggccgacgt actcgtggcg ccagttctcg gatgcgagct
     1621 cgtggcccgc gctgcggatc tccttcgcga ggtccgggaa ggcctcgacg ccggcgccgt
     1681 tcaggaacca cgaggtgtgg atgccggctt cctcgaggat gtcgagcatg cggtacgcgc
     1741 cgcagcgctc gccgtagaga cggtcggtga tgagagcgat gtcggtcgtg aagagcgagc
     1801 ccttggggaa gtcatcacgg aactgcgcct gcgccgagcc cggcttgccg agctcttcgg
     1861 tgggccaggt ctcgaggacg atcgtcggca tgacggcgat gcgcgcgtcg ttgggccact
     1921 ggatcgggcg ccgaggcagt cgcatgtgcg ttccctccaa agcgatatcg cgatggttcg
     1981 gcaattccgt gtcagggtcg tgtcccggtc agtcctcctc cgcgtatgac cgggcgagcg
     2041 gccgaggctg ccacccgtcg tcgcggatga gcacgcattg ctccgcgggc atgcggatcc
     2101 acaccttctg gtcgcgcacc agtcgctgcg cgggatgggc acgcgcgcgg acgagctcgc
     2161 cggccacgtc gaccagacag tcgaggtggt cacccaggaa cgcggccacg cgcagcgtcc
     2221 cctcgaactc gttctcgacg cccttcggct cgacgtcgac gtacacgttc tccggccgca
     2281 ggacgagatg cgcctgcgaa cccacgggga ggtcgtccgg aggcgcgcag ctcacgcggc
     2341 caccgagcgc gcgcacgccg ccgcggtcga tctcggcgcg cacgaacgtg ccgatgccga
     2401 cgaagtcggc gacgaagccg gagctcggcg aggtgtaaat gtcgcgtggc ccgccctcct
     2461 gcacgatgtt gc
//
LOCUS       DASTZR010000195         2282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_454898, whole genome shotgun sequence.
ACCESSION   DASTZR010000195 DASTZR010000000
VERSION     DASTZR010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454898"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /gene="argH"
                     /locus_tag="VFV20_04335"
     CDS             <1..859
                     /gene="argH"
                     /locus_tag="VFV20_04335"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU5287608.1"
                     /translation="VEALRRDRARIEEARARTAVSPLGAGALAGVPYPVDPAAVAKDL
                     GLERTFRNSIDAVADRDFISDFVYASALTAVHASRLAEELVLWTAAEFGYAEFSDEHA
                     TGSSIMPQKKNPDVAELVRGRASRIIGDVVAILASMKGLPLAYDGDLQEQRVPLYDAA
                     ATVPALDALATVIRGLRFDRERMRGATERGMLAATDLADHLARKGVPFREAHEITGRV
                     VRARLAKGADLAGLTLDELRAVDPRFDPSALDEIATPRSLAARSSPGGTAPDRVRVAL
                     AEARKTLRG"
     gene            856..1392
                     /locus_tag="VFV20_04340"
     CDS             856..1392
                     /locus_tag="VFV20_04340"
                     /inference="COORDINATES: protein motif:HMM:NF005840.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEU5287609.1"
                     /translation="MTVRIREARAQDVESMRELIDRYAKEDRMLSRSRDFLLEHLRDF
                     YVAEDGDEFLGCCAIAVLTNDLAEIRSLAVRPDAGGRGVGKALVEACVEEARRLGLRR
                     VFALTLVPAFFERCGFTLTSLGRLPEKSASECPVCPKRYACDEHAMLLHLDGTTPAPL
                     RPGDPWGYTRIFLGREPE"
     gene            1442..1813
                     /locus_tag="VFV20_04345"
     CDS             1442..1813
                     /locus_tag="VFV20_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287610.1"
                     /translation="MGRTGRTLIAGFDAVVMGAAAYTVTSGRFALAPAELILWGSALA
                     AAVCAAVVYTNGSPLVAWGAIGYVLFGGLLTDGGPNLALLALALALTPLVPRPRGSVW
                     LGIVVALAVAVGARVAIGFVV"
     gene            1946..>2282
                     /locus_tag="VFV20_04350"
     CDS             1946..>2282
                     /locus_tag="VFV20_04350"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU5287611.1"
                     /translation="MPSPIALLARVRHSFRLLNRAIAEGSRRAGLTVQQQGFLLAVAA
                     RGGRNVPLSHVRAELLMDQATASELLRRLAERGLVRRAPGEDRRALRVTLTADGRATF
                     KVSVAEIGAA"
BASE COUNT          280 a          834 c          833 g          335 t
ORIGIN      
        1 cgtcgaggcg ctgcgtcgcg atcgcgcccg catcgaagaa gcgcgcgcgc ggaccgcggt
       61 gtcgccgctc ggcgccggcg ccctcgcggg tgtgccctat ccggtggatc ccgccgcggt
      121 ggcgaaggac ctcggtctcg agcgcacctt ccgcaacagc atcgacgccg tcgcggaccg
      181 ggacttcatc agcgacttcg tctatgcctc cgccctcacc gcggtgcacg cctcacggct
      241 ggcggaggag ctggtgctgt ggaccgccgc ggagttcggc tacgcggagt tctcggacga
      301 gcacgcgacc ggatcgagca tcatgccgca gaagaagaac cccgacgtcg ccgagctggt
      361 ccgcgggcgc gcgtcccgca tcatcggcga tgtcgtcgcg atcctcgcct cgatgaaggg
      421 tctgccgctc gcgtacgacg gcgacctgca ggagcagcgc gtgccgcttt acgacgccgc
      481 ggcgaccgtc ccggcgctgg acgcgctcgc cacggtcata cgcggccttc gcttcgatcg
      541 cgagcgcatg cgcggcgcga ccgagcgcgg gatgctggcc gcgaccgatc tcgcggacca
      601 tctcgcgcgg aagggcgtgc ccttccgcga ggcgcacgag atcaccggac gcgtcgtccg
      661 cgcgcggctc gcgaagggcg ccgatctcgc cgggctgacg ctcgacgagc tccgcgccgt
      721 cgatccgcgc ttcgacccgt cggcgctgga cgagatcgca acgccgcgat cgctcgcggc
      781 gcgctcgtcg ccgggtggca ccgcgccgga ccgcgtgcgc gtggcgctcg cggaggcgcg
      841 caagaccctc cgcggatgac ggtccgcatc cgcgaggctc gcgcgcagga cgtcgagtcg
      901 atgcgcgagc tgatcgaccg ctacgcgaag gaggaccgca tgctctcgcg ctcgcgcgac
      961 ttcctcctcg agcacctgcg cgacttctac gtggccgagg acggcgacga attcctcggc
     1021 tgctgcgcga tcgcggtgct gacgaacgac cttgccgaga tccgctcgct ggcggtgcga
     1081 ccggatgccg gaggaagggg cgtgggcaag gcgctggtcg aggcgtgtgt cgaggaggcg
     1141 cggcgcctcg gcctccgccg cgtcttcgcg ctcacgctcg tgccggcgtt cttcgagcgc
     1201 tgcgggttca ctctgaccag cctcgggcgg cttcccgaga agagcgcgtc cgaatgcccg
     1261 gtctgcccca agcgatacgc gtgcgacgag catgcgatgc tgctgcacct cgacggcacg
     1321 acgccggcgc cgctgcgtcc cggcgatccc tggggctata cgcgcatctt cctcggacgc
     1381 gagcccgagt gatgccccgc gccgcctgac gcgcggcgcg gccggccctt accctcgaac
     1441 ggtgggacgg acgggacgca ccctcatcgc cggattcgac gcggtcgtca tgggcgcggc
     1501 cgcgtacacg gtgacgtccg gtcgcttcgc gctggcgccc gccgagctga tcctctgggg
     1561 atccgcgctc gcggcggccg tttgcgccgc ggtcgtgtac acgaacggct cgcccctcgt
     1621 cgcgtggggc gcgatcggct acgtgttgtt cggcggcctg ctgacggacg gcggccccaa
     1681 cctcgcgctg cttgccctcg cgctcgcgct caccccgctc gtgccgaggc cgcgcggctc
     1741 ggtctggctg gggatcgtcg tcgcactggc cgtcgcggtc ggagcgcgcg tcgccatcgg
     1801 gttcgtcgtg taggggtgcg ccgcgggccc cgcgccacca ggggcccgcg gcgctgggag
     1861 gagtcgctca ggactcgcgc ggcgcaaatt acttcgtgag acgaaccgaa gtcaaggcgt
     1921 cggcgggtgc cgtagcgtag cccccatgcc gagcccgatc gcgctgctcg cccgcgtccg
     1981 gcactccttc cggctgctga acagagcgat cgccgagggt tcccgcaggg cgggattgac
     2041 cgtccaacag caggggttcc tgctggccgt cgccgcgcgc ggcggtcgga acgtgccgct
     2101 ctcgcacgtc cgcgccgagc tgctgatgga tcaggcgacg gcgagcgagc tgctccgcag
     2161 gctcgccgag cgcgggctcg tgcggcgcgc tcccggcgag gaccgccgtg cgctgcgcgt
     2221 gacgctcacg gcggacggtc gagcgacctt caaggtgtcg gtcgccgaga tcggcgcggc
     2281 cc
//
LOCUS       DASTZR010000196         5073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_817839, whole genome shotgun sequence.
ACCESSION   DASTZR010000196 DASTZR010000000
VERSION     DASTZR010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817839"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VFV20_04355"
     CDS             <1..285
                     /locus_tag="VFV20_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5287612.1"
                     /translation="TALAVARAVRPDLILLDLHIPKLDGWGFVKRFREEEWAAKVPVV
                     AVSASATPPERDRALRTGCDDFLPKPYYPEQMRDMLQKHLKPRAHSETGR"
     gene            342..1334
                     /locus_tag="VFV20_04360"
     CDS             342..1334
                     /locus_tag="VFV20_04360"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5287613.1"
                     /translation="MTRARLRGLVLGLLVVGVIGAVDFATGPDYGFAFFYFAPIVPVA
                     WRFGMVPGLVVAVAAAVAWFGADVAQRPAQAVLPVAWNALSRLAIFIGGAWLIDRVKQ
                     DRTRMALIDAQRAEFLRVLEHELPVPAQDMVTALNEAQASGTFGVADIQALRHRAESL
                     LFLTRDFVALGQLQNKRLQMRAVPVDIAQLVTEVSRERPDHGSVLVTVPGDGLIVTAD
                     PDRLRQVLVNTIAQVVRDAGAIDYVSITVRARPGEALISVSAAMPATATRAEQGHDIT
                     VGLQLAQLLIEAMGGSLDVERAAIGRGSRVTLRLPVTPAVPNAQLQPADAPNTH"
     gene            complement(1348..2358)
                     /locus_tag="VFV20_04365"
     CDS             complement(1348..2358)
                     /locus_tag="VFV20_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287614.1"
                     /translation="MNVLLPLLSSVTSFVFAALVFTQWSRRHHAFQLVWALGLLWYGI
                     SAGTEFWGSAFGWSEPLYRAWYLIGAFYVAAWLGMGTIALLARTRFGYFVAGSLLLSA
                     LITLAAGAKYAASGSSAAAAAACVVVAIATAAATWRRRDLSALVPGAGLVLGSIAVAV
                     LVLSADVAAPGYALDPVTGVPVGEAMPAALRPLTIPFNVGGALALVCGAVFSAYVYMP
                     KRRLLHGRRLPPVVAQLYGALAVVVNLAASLPSAAGALLRGELHSRVPATLLIAVGGF
                     IPGVTSGLNRFGITWSFFLGELLGVLFIFAGFIVSTEVFARMRVGPFAIGRTERSAET
                     TA"
     gene            2444..2701
                     /locus_tag="VFV20_04370"
     CDS             2444..2701
                     /locus_tag="VFV20_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 family protein"
                     /protein_id="HEU5287615.1"
                     /translation="MCRSIRTLRGAEPATDEEIRAAARQFVRKVSGFREPSAKNAEAF
                     EAAVAEIEGATSRLIERLPRSKAVRSPAQRPSNGVRRSTPR"
     gene            2769..3611
                     /locus_tag="VFV20_04375"
     CDS             2769..3611
                     /locus_tag="VFV20_04375"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU5287616.1"
                     /translation="MKNVLRLFAIGTVLALVASGCGGALPLQSKPTEDPLAGAYIAKG
                     GGGAIDHIRALTTAFSKIHPTVVWQGFDDVGSDAGVKLAVDGLVDIGYISREPKANEK
                     GTVETLSIGASGTGVGVNASNTVKALTKEQVRKIFAGEITDWKDVGGTPGKIRVLLRE
                     PESATRSAFEGYFFGKDKPAYSKDAIEVFQIDETQKAITSFKDAIGMMTMNAQTLGST
                     EIRLLTIDGIGATRENLTNGTYPIRRPLYIVYNTDKTKVKPAMREFIDWVKGPEGQKV
                     LASL"
     gene            3620..>5073
                     /locus_tag="VFV20_04380"
     CDS             3620..>5073
                     /locus_tag="VFV20_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEU5287617.1"
                     /translation="MRFFGQLFAPSLLRALVGRTVGAAVAALFILGTVALYESNELVT
                     TQFEDEANVVAQTAANEIDDQASLMIRAASLIAGLPTTREFTEARDSAGLQSFLIPQK
                     SRLGVDIMNVADITGAYIAGAQDVKAGDTLRPELLALTRAGAQQSWVLYDEPEGLVIR
                     AIFVIRGADQDPIGVAEIGSVLGNAFLRGINTKSDAQLALVWNGKVRGSTFEGADSKP
                     LAIDGGLFPTVDEVDTAERDTLFKDISINGTDYYGIFRVVRSQRQNPGILAVLVPTAA
                     VVNAQRTLLGIMVVLAVALMGLVIFFAYRSATAMTTPLANLARAAQRIESGDLQVSVQ
                     QRSGHEIGTLERAFDTMARSLHDRERAQQEYLAEVRTVNEVADAVVGVTDRERIFAES
                     LSRMVTLFQADGASIVIREDPPGAPSGSGGRLVPATVMNVDPEVAVAIATRVLVSSVH
                     DPNRIQHAQVAGSPLPYVAHIPLSARGRAVGLLSA"
BASE COUNT          853 a         1756 c         1728 g          736 t
ORIGIN      
        1 accgcgctcg ccgtcgcacg ggcggtccgc cccgatctca tcctcctcga cctgcacatc
       61 ccgaagctcg atggctgggg cttcgtgaag cgtttccgcg aggaggagtg ggccgccaag
      121 gtgcccgtcg tcgcggtctc agcgtcggcg acgccgcccg agcgggatcg cgcgctccgc
      181 accggatgtg acgacttcct cccgaagccc tactaccccg agcagatgcg ggacatgctc
      241 cagaagcatc tgaagccgag agcccacagc gaaacggggc gctgaccgtt ccactttcgg
      301 gggatggcac gccgtccccg ggagggcgca gcatgccggg aatgaccaga gcgagactgc
      361 gcggcctcgt cctcggcctg ctcgtggtgg gtgtcatcgg cgccgtcgac ttcgcgaccg
      421 gcccggacta cggcttcgcc ttcttctact tcgccccgat cgtcccggtg gcatggcgct
      481 tcggcatggt gccggggctg gtcgtcgccg tcgccgccgc ggtcgcgtgg ttcggagcgg
      541 atgtggcaca gcgtccagcg caggcggtcc ttccggtggc ctggaacgcg ctgagccgcc
      601 tcgcgatctt catcggcggc gcgtggctca tcgatcgcgt caagcaggac cgcacccgga
      661 tggcgctcat cgacgcgcag cgcgccgagt tccttcgcgt gctcgagcat gagctgccgg
      721 tgcccgcgca ggacatggtc acggcactga acgaggcgca ggcgagcggg acattcggcg
      781 tcgcggatat ccaggcgctc cggcaccgcg cggagtcgct gctattcctg acgcgcgact
      841 tcgtcgctct cggccagctc cagaacaagc gtctccagat gcgcgccgtg cccgtggaca
      901 tcgcgcagct cgtcaccgag gtctcgcgcg agcgccccga tcacggttcg gtcctcgtga
      961 cggtgcccgg cgatggcctg atcgtcacgg ccgaccccga tcgccttcgt caggtactcg
     1021 tcaacaccat cgcgcaggtc gtccgcgacg ccggagccat cgactacgtg tcgatcaccg
     1081 tccgcgcccg acccggcgag gcgctcatct cggtgtccgc ggccatgccc gcgaccgcga
     1141 cgcgagccga gcagggccac gacatcacgg tgggtctcca gctcgcgcag ctgctcatcg
     1201 aggcgatggg tgggagcctc gacgtcgagc gcgcggcgat cggccgaggc agccgcgtca
     1261 cgctgcgcct gccggtcaca ccggcagtgc cgaacgcgca gctgcagccc gccgacgcac
     1321 cgaacacgca ctgaccgcac aggctcgtta cgccgtcgtc tcggcgctcc gctccgtccg
     1381 tccgatcgcg aaaggcccga cgcgcatccg cgcgaagacc tcggtgctca cgatgaaccc
     1441 cgcgaagatg aagagcacgc cgagcagctc accgaggaag aacgaccagg tgatcccgaa
     1501 acggttcagc ccgctcgtca cgccgggtat gaagcccccg acggcgatga gcagggtggc
     1561 gggcacccgc gaatggagct cgccacgcaa cagcgcaccg gcggcggagg gaagcgaggc
     1621 ggcgagattg acaacgaccg cgagcgcccc gtagagctgc gcgaccacag gcggcaaccg
     1681 acgtccatgc aggagccttc gcttcggcat gtacacgtag gcggagaaga ccgcgccgca
     1741 cacgagcgcg agcgccccgc cgacgttgaa cgggatcgtc agcggtcgca gcgcggccgg
     1801 catcgcctcg ccgaccggca cgccggtcac cgggtcgagc gcgtagccgg gcgccgccac
     1861 gtccgccgag aggacgagga ccgcgaccgc gatcgagccg agcacgagcc cggcgcccgg
     1921 gacgagggcg ctcagatcgc ggcggcgcca ggtcgccgcg gcggtcgcga tggcgacgac
     1981 cacgcaggca gcggcggcgg ccgcggacga gcccgaggcc gcgtacttcg cgccggcggc
     2041 cagcgtgatg agggcggaga gcaggagcga cccggcgacg aagtagccga agcgcgtgcg
     2101 cgcgagcagc gcgatcgtgc ccatcccgag ccacgccgcg acgtagaagg cgccgatgag
     2161 ataccaggcc cgatagagcg gctcgctcca gccgaacgcg ctgccccaga actccgtgcc
     2221 ggcgctgatc ccgtaccaga gcaggccaag cgcccacaca agctggaagg cgtgatgccg
     2281 gcgcgaccac tgcgtgaaga cgagggcggc gaagacgaag ctcgtcaccg atgacaggag
     2341 ggggaggagc acgttcattg acatggcggc acgttcgttc gcgcgtgcag cgtcgtcaaa
     2401 tcacctaccg agattcgctt aggaaggggt attttctagg ccgatgtgca ggagcatccg
     2461 cacgctgaga ggggccgagc ccgcgaccga cgaggagatc cgggccgctg cacgccagtt
     2521 cgtacggaag gtgtccggtt ttcgcgagcc gtcggcaaag aacgccgagg cgttcgaggc
     2581 cgcggtggcg gagatcgagg gggcgacatc ccgtctcatc gagcggctgc cgcgctcgaa
     2641 agctgtgagg agcccggctc agcgtccgtc aaatggcgta cgccgctcta ctccccgtta
     2701 gggggatggc gaccacctct gattggacga gactggcgcc cgcaagcggg ctcggtagga
     2761 ggcgaagggt gaagaacgtt ctgcggctgt tcgctattgg tacggtgctg gcgctcgtgg
     2821 cgtccggctg tggcggggcg ctgccactcc agtcaaagcc caccgaggac cctctcgccg
     2881 gtgcgtacat cgcgaagggt ggcggcggcg cgatcgacca catccgcgcg ctcacgaccg
     2941 ccttttcgaa gatccatccg acggtcgtgt ggcagggctt cgacgacgtc ggatccgacg
     3001 cgggcgtgaa gctcgcggtc gacggtcttg tcgacatcgg ctacatcagc cgcgagccga
     3061 aggcgaacga gaagggcacg gtcgagacac tctccatcgg tgcctcgggc accggcgtgg
     3121 gcgtgaacgc gagcaacact gtgaaggcgc tcacgaagga gcaggttcgc aagatcttcg
     3181 ccggtgagat caccgattgg aaggacgtcg gcgggacgcc cggcaagatc cgcgtcctcc
     3241 tgcgtgagcc cgagtcggcg acgcgctcag cgttcgaggg ctacttcttc ggcaaggaca
     3301 agccggcgta ctcaaaggac gccatcgagg tgttccagat cgacgagacg cagaaggcga
     3361 tcacctcgtt caaggacgcc atcggcatga tgaccatgaa cgcgcagacg cttggatcga
     3421 ccgagatccg cctgctcacc atcgacggca tcggggcgac gcgggagaac ctcaccaacg
     3481 gcacctaccc gatccgccgt ccgctctaca tcgtctacaa caccgacaag acgaaggtga
     3541 agccggcaat gcgcgaattc atcgattggg tgaagggtcc cgaaggccag aaggtcctcg
     3601 cgagcctcta gccggaacga tgcgtttctt cgggcagctc ttcgcgccga gcctcctacg
     3661 ggcgctcgtc ggccgcaccg tcggtgccgc cgtcgcggcg ctcttcattc tcggcaccgt
     3721 cgcgctgtac gagtcgaacg agctcgtcac gacgcagttc gaggacgagg ccaatgtcgt
     3781 cgcgcagacc gccgccaacg agatcgacga ccaggcctct ctcatgatcc gcgccgcctc
     3841 gctcatcgcc ggccttccca cgacgcgcga gttcacggag gctcgcgaca gcgcggggct
     3901 ccagtcgttc ctcatcccgc agaagtcgcg cctgggcgtg gacatcatga acgtcgccga
     3961 catcaccggc gcgtatatcg ccggcgcaca ggacgtgaag gccggcgaca cattgcgtcc
     4021 ggagctgctc gcgctgacgc gcgcgggcgc tcagcagtcg tgggttctct acgacgagcc
     4081 cgagggtctc gtcattcgcg ccatcttcgt gatccgcggc gccgaccagg accccatcgg
     4141 cgtcgccgag atcgggtcgg tgcttggcaa cgcgttcctg cgcggcatca acacgaagag
     4201 cgacgcccag ctcgcgctgg tctggaatgg aaaggtgcgc ggatcgacct tcgagggagc
     4261 ggatagcaag ccgctcgcca tcgacggcgg cctcttcccg acggtcgatg aagtcgacac
     4321 ggcggagcgc gacacgctct tcaaggacat ctcgatcaac gggaccgact actacggcat
     4381 ctttcgcgtc gtgcgctcgc agcgccagaa ccccggcatc ctcgctgttc tcgtgcccac
     4441 ggcggcggtc gtgaacgccc agcggacact tcttgggatc atggtcgtcc tcgccgtcgc
     4501 actgatgggg ctcgtcatct tcttcgcgta ccggtcggcg acggcgatga cgactcccct
     4561 ggcgaacctc gcgcgcgccg cgcaacgcat cgagtcgggc gacctgcagg tctcggtgca
     4621 gcagcgctcg ggtcacgaga tcggcaccct cgagcgcgcg ttcgacacga tggcgcgctc
     4681 gctccacgac cgcgagcgcg cgcagcagga gtacctcgcc gaggtgcgca cggtgaacga
     4741 ggtcgcggac gccgtcgtcg gtgtcacgga ccgtgagcgc atcttcgccg agtccctgtc
     4801 tcgcatggtg acgctgttcc aggccgacgg cgcgtcgatc gtgatccgag aggacccgcc
     4861 tggtgcgccg agcggctccg gcggccgact agtgcccgcc accgtcatga acgtcgatcc
     4921 ggaggtggcc gtcgcgatcg cgacgcgcgt cctcgtctcg agcgtccacg acccgaaccg
     4981 catccagcac gcccaggtcg cgggctcacc gctgccgtat gtcgcgcaca tcccgctgtc
     5041 ggcgcgcggc cgcgcggtcg gtctgctgtc cgc
//
LOCUS       DASTZR010000197         4073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_898977, whole genome shotgun sequence.
ACCESSION   DASTZR010000197 DASTZR010000000
VERSION     DASTZR010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898977"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..741
                     /locus_tag="VFV20_04385"
     CDS             100..741
                     /locus_tag="VFV20_04385"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEU5287618.1"
                     /translation="MQHIPSERDGPFEFIAGSLAVDFTNTLSGTRERPGTEHVARYAD
                     LVEFARQGELIRAVDAKRLLAEAERHPQKATEMYRRAIALREAIWRAFDRIAQDREPA
                     PEDVAAISAEAAQAFANARVTRRGTSYGWDWPETDDLARPLWPIARSAVDVLTSDAER
                     ARLRECASDTCGWLFVDRTKNGSRRWCDMDDCGNRAKVRAYRERRARSARKAR"
     gene            770..1609
                     /locus_tag="VFV20_04390"
     CDS             770..1609
                     /locus_tag="VFV20_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020501857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEU5287619.1"
                     /translation="MTKVKAALAQVAWTGDKDSMIQKHVKYIEDAAKQGAQAMCLQEL
                     FYGPYFCQVQDTKHYSYTEKIPDGPTTKLMQELAKKHSMVLVVPMYEEDQTGIYYNTA
                     AVIDADGKYLGKYRKTHIPHVNGFWEKFYFRPGNLGYPVFETAVGKVGVYICYDRHFP
                     EGARMLGLHGAELVFIPSATSRGLSMHLWSIEQTAHAIANGYWVGTINRVGKEMEFGP
                     NDYYGSSYFADPRGKITKQASDTNEELVVADLDLELVREVRNTWQFYRDRRPDMYDDL
                     VKP"
     gene            1636..3111
                     /locus_tag="VFV20_04395"
     CDS             1636..3111
                     /locus_tag="VFV20_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012689547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5287620.1"
                     /translation="MTTATATARDQRMLIGGEWVDAADRRTFAVETPARRGEVIAQVP
                     RAGAADVDRAVRAAHAAFPEWRAMVPRERGRILTRIADDLDAAVEDLARLCASETGNA
                     IRTQARPEAKTTADLFRYFGGLGGELKGETVPLGDANFSYTRREPWGVVAAIVPWNVP
                     ILISAWKLAPALLAGNTVVLKPSANAPLAALALARICDRHLPKGVLSVVTGTGDEVGT
                     PLAEHALIAKISFTGNTETGKAILRAAADRILPVTLELGGKSPQIVFADSDNDRTADG
                     VIAGMRFARQGQSCTAGSRLFVHQSVFDSFLERLATKLRKMKVGDPLDEASDMGAIVS
                     RQQFESVCRYIDEGTKQEGARTVVGGLPPKSGPLAAGYYIEPTVFASERNDWRLAREE
                     IFGPVLVAIPWTDEADAIRMANDSHYGLAAYVWCNDITRALRTAHALDAGWIQVNQGG
                     GQVVGQPYGGYKQSGLGRENSLEGMLESFTQRKTVNVNLTA"
     gene            3113..3523
                     /locus_tag="VFV20_04400"
     CDS             3113..3523
                     /locus_tag="VFV20_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEU5287621.1"
                     /translation="MELTTVALEPTPVMVVRDAGEMPDAAKRAFEKLEAALPTLRGRH
                     FYGYWDPDAKVYVACVAAIAADDAERMGLERATIPGGRYQRARLKGEPDAIYPRIGPT
                     FEEMGKQITDVDHGRPWLEFYRARDVIDLLVPVK"
     gene            3603..>4073
                     /locus_tag="VFV20_04405"
     CDS             3603..>4073
                     /locus_tag="VFV20_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287622.1"
                     /translation="MPAALDLRNRTWITAHALPLTTGAAGSVLAARVEDSLDRAYESR
                     WYVLKGLSAGDDVTVTLTLPPGFTEDYTLLGFTDIRVMAARLLAQLGTTGPLDADDMD
                     SDDMDSDDMDSDDMDSDDMDSGDLGSDPISEIVRQQLSDVYASAQRHSLRSASVT"
BASE COUNT          712 a         1391 c         1405 g          565 t
ORIGIN      
        1 gacaccggct atgagcgata tagcaggtgt cttgaccagt cgaagcctac tccctacact
       61 gacaccaatc aaccggcgat aggcggtgtc aacagttaga tgcagcacat tccctccgag
      121 cgcgacggcc cgttcgagtt catcgccggc tccctggctg tcgatttcac gaacaccctc
      181 tcggggactc gcgagcgccc tggcacggag cacgtcgccc gatacgcgga cctcgtcgaa
      241 ttcgcacggc agggtgagct gatccgggcg gtggacgcga aacggctgct cgcggaggcg
      301 gagcgccatc cccagaaggc cacggagatg tatcgccgtg cgatcgcgct gcgtgaggcc
      361 atctggcgtg cgttcgatcg catcgcccag gaccgcgaac ccgcgccgga ggacgtcgcc
      421 gccatcagcg ccgaggccgc gcaggccttc gccaacgccc gcgtgacccg tcgcggcacg
      481 agctacggct gggactggcc cgagacggac gacctcgcac ggccgctctg gccgatcgcg
      541 cggagcgcgg tcgacgtgct gacctccgac gccgaacgcg cccgcctgcg ggagtgcgcg
      601 tccgacacgt gcggatggct cttcgtcgat cgcacgaaga acggaagtcg ccgctggtgc
      661 gacatggacg attgcggcaa ccgtgccaag gtgcgcgcct accgcgagcg acgcgcgcgc
      721 agcgcccgca aggcgcgcta acctagcggc tcctacaagg ggggcaaaga tgacgaaggt
      781 gaaggcggcg ctcgcgcagg tcgcgtggac cggcgacaag gactcgatga tccagaagca
      841 cgtgaagtac atcgaggacg ccgcgaagca gggcgcgcag gcgatgtgcc tccaggagct
      901 cttctacggt ccgtacttct gccaggtgca ggacaccaag cactactcgt acaccgagaa
      961 gatccccgac ggcccgacga cgaagctcat gcaggagttg gcgaagaaac actcgatggt
     1021 gctcgtcgtg ccgatgtacg aggaggacca gaccggcatc tactacaaca ccgccgcggt
     1081 catcgacgcg gacgggaagt acctcggcaa gtaccgcaag acgcacatcc cgcacgtgaa
     1141 cgggttctgg gagaagttct acttccgtcc cggcaacctc gggtatccgg tcttcgagac
     1201 cgcggtcggc aaggtcggcg tgtacatctg ctacgaccgc cacttcccgg agggcgcgcg
     1261 catgctcggg ctccacggcg ccgagctcgt gttcatcccc tccgcgacct cgcgcgggct
     1321 ctcgatgcat ctctggtcca tcgagcagac cgcgcacgcg atcgcgaacg gatactgggt
     1381 cggaacgatc aaccgcgtcg gcaaagagat ggagttcggg ccgaacgact actacggctc
     1441 ttcctacttc gccgacccgc ggggcaagat caccaagcag gcgtcggaca cgaacgaaga
     1501 gctcgtcgtc gccgatctcg acctggagct cgtgcgcgag gtccgcaaca cctggcagtt
     1561 ctatcgcgac cgccgtcccg acatgtacga cgatctcgtg aagccctgac ggggcgcggg
     1621 tttggagcga gtgcgatgac cacggcgacc gcgacagcga gggaccagcg catgctcatc
     1681 ggcggcgagt gggtggatgc cgccgaccgc cgcacgttcg cggtggagac gccggcgcgg
     1741 cgcggcgagg tcatcgccca ggtgccgcgc gcgggcgccg ccgacgtcga tcgcgccgtc
     1801 cgtgccgcgc acgcggcgtt cccggagtgg cgtgcgatgg tgccacgcga gcgcggccgg
     1861 atcctcacgc gcatcgccga cgacctcgac gcggcggtgg aggacctcgc gcgcctctgc
     1921 gcgtcggaga ccggcaacgc catccgcacg caggcgcggc ccgaggcgaa gaccacggcg
     1981 gacctcttcc gctacttcgg cgggctcggc ggcgagctga agggcgagac cgtcccgctc
     2041 ggcgacgcga acttctcata cacgcggcgc gagccgtggg gcgtcgtcgc cgcgatcgtc
     2101 ccctggaacg tcccgatcct catcagcgca tggaagctcg cgcccgcgct cctcgcgggg
     2161 aacaccgtcg tgctgaagcc gtcggcgaac gcgccgctcg cggcgctggc gctcgcgcgg
     2221 atctgcgatc gccatctgcc gaagggcgtg ctgagcgtcg tcaccgggac cggcgacgag
     2281 gtcgggacgc cgctcgccga gcacgcgctc atcgcgaaga tctcgttcac cgggaacacc
     2341 gagaccggga aggcgatcct ccgtgccgcg gcggaccgca tcctgccggt cacgctcgag
     2401 ctcggcggca agagcccgca gatcgtcttc gccgacagcg acaacgatcg caccgccgac
     2461 ggggtcatcg ccggcatgcg cttcgcgcgc cagggccagt cgtgcacggc gggctcgcgg
     2521 ctcttcgtgc accaaagcgt cttcgactca ttcctcgagc ggctcgcgac gaagctgcgc
     2581 aagatgaagg tcggtgaccc gctcgacgag gcgagcgaca tgggcgcgat cgtgagccgg
     2641 cagcagttcg agagcgtgtg ccgctacatc gacgagggca cgaagcaaga gggcgcgcgc
     2701 accgtcgtcg gcgggctccc gccgaagagc ggtccgctgg ccgcggggta ctacatcgag
     2761 ccgacggtgt tcgcgagcga gcgcaacgac tggcgcctcg cgcgcgagga gatcttcggg
     2821 ccggtgctcg tggccatccc ctggaccgac gaggccgacg cgatccggat ggcgaacgac
     2881 agccactacg ggctcgccgc gtacgtgtgg tgcaacgaca tcaccagggc gctgcgcacc
     2941 gcgcacgcgc tcgacgccgg ctggatccag gtgaaccagg gcggcggcca ggtcgtcggg
     3001 cagccgtacg gcggctacaa gcagagcggg ctcggccgcg agaactcgct cgagggcatg
     3061 ctcgagtcgt tcacgcagcg caagaccgtc aacgtcaacc tcacggcgta gcgtggagct
     3121 caccacggtc gcgctcgagc cgaccccggt gatggtcgtc cgtgacgccg gcgagatgcc
     3181 cgacgcggcg aagcgggcgt tcgagaagct cgaggccgcg ctcccgacgc tgcggggccg
     3241 gcacttctac gggtattggg atccggacgc gaaggtctac gtcgcctgcg tcgccgcgat
     3301 cgcggctgac gacgccgagc gcatgggtct cgagcgggcg acgatcccgg gcggccggta
     3361 ccagcgggcg cggctcaagg gggagcctga cgccatctac ccgaggatcg ggccgacgtt
     3421 cgaggagatg gggaagcaga tcacggacgt cgaccacggg cggccctggc tcgagttcta
     3481 ccgcgcgcgc gatgtgatcg atctcctggt gccggtgaag tgagcgacgc gcagcccgcg
     3541 gggtggctgc tcatcgccgc gacggcggcg acaacccttg acgatgtccc tagagtcgcg
     3601 ccgtgcccgc cgcgctggat ctcaggaaca ggacgtggat caccgcccac gcgttaccgc
     3661 tcaccaccgg agccgcgggc agcgtattgg cagcccgcgt cgaggactcg ctcgatcgtg
     3721 cctacgaatc acggtggtac gtcctcaaag gactgagtgc cggtgatgac gtcaccgtca
     3781 cgctgacgct acccccgggc ttcaccgagg actacacgct cctcggattc accgacattc
     3841 gtgtcatggc cgcgcggctg ctcgcacagc tcgggaccac aggccccctc gacgcggatg
     3901 acatggactc ggacgacatg gacagcgacg acatggactc ggacgacatg gacagcgacg
     3961 acatggattc gggcgacctg ggctccgacc cgatcagcga gatcgtcagg cagcagctga
     4021 gcgacgtcta tgcgagcgct cagcggcact cactgcggtc cgcatcggtg acg
//
LOCUS       DASTZR010000198         2089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_507555, whole genome shotgun sequence.
ACCESSION   DASTZR010000198 DASTZR010000000
VERSION     DASTZR010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507555"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VFV20_04410"
     CDS             complement(<1..364)
                     /locus_tag="VFV20_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287623.1"
                     /translation="MRALVRIAAIAVAGAVLYYAGLVNSIVLQRPERPGAAVVVLAIG
                     AALLVLAGALRGTGDGDSASPPRSVLAHRLTWLFVCVMATVSIAWLFTMPRQHSDDWT
                     PYHNDAIALNECAARSLLQ"
     gene            complement(368..1462)
                     /gene="mnmA"
                     /locus_tag="VFV20_04415"
     CDS             complement(368..1462)
                     /gene="mnmA"
                     /locus_tag="VFV20_04415"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEU5287624.1"
                     /translation="MSRVFVAMSGGVDSSVAAALLAERGEDVVGVWMRLTPAGTSGDA
                     PRCCGTDEAGEDARRAAAVLGIPFYALDYAELFGREVVDRFVDSYAAGETPNPCVACN
                     HYVKFEALMRDVVAKFGADRLATGHYARVEHGGRHRLLRARDAAKDQSYALYMLGQRE
                     LARLELPVGELADKSETRALASRFGLPTASKPDSQDICFIGGDYRGFVRERAPGAFVR
                     GPVERLDGTVVAEHGGIGTLTIGQRSGVGVASGERMYVLRLEPDRRAAIVGTREEISA
                     TCYELRDVRFTGDATPAERFRTEAVLRYRGTPLAGWVGVQDDRAFLDLDAPALVAPGQ
                     AAVFYDGDAVVGGGIVARAADATGRATLAG"
     gene            complement(1459..1767)
                     /locus_tag="VFV20_04420"
     CDS             complement(1459..1767)
                     /locus_tag="VFV20_04420"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEU5287625.1"
                     /translation="MTSGTGSDANPRDGDRVTFTVEVVDRRVRIVGTELNACAAANRA
                     AEEIRQRVGGEIADEIARIQVRDIIETLRLDADGERCALTVLSALQAAIVDARVKDLP
                     "
     gene            complement(1764..>2089)
                     /locus_tag="VFV20_04425"
     CDS             complement(1764..>2089)
                     /locus_tag="VFV20_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5287626.1"
                     /translation="VPDAVLTGHRTERLPNNASFCFRGADGPSLVMALDLEGFAVSSG
                     SACTSGTTEPSHVLLALGLDESTAKGSLRVSVGRGTSEADVDAFIAALPAIVARVRGA
                     SIVTA"
BASE COUNT          307 a          778 c          741 g          263 t
ORIGIN      
        1 cctgcagcag gctgcgagcg gcacactcgt tcagggcgat ggcgtcgttg tgatacggcg
       61 tccagtcgtc cgaatgctga cgcggcatcg tgaacagcca cgcgatcgag acggtggcca
      121 tgacgcacac gaagagccac gtgaggcgat gcgccagcac agagcgcggc ggcgacgcgc
      181 tgtcgccatc gccggtcccg cggagcgcgc cggccagcac gaggagcgcg gcaccgatcg
      241 ccaggacgac cacggccgcg ccgggccgct ccggccgctg cagcacgatg ctgttgacca
      301 ggccggcgta atagagcacg gcaccagcca cggcgatcgc ggcgatacgg acgagggcgc
      361 gcaccggcta gcccgcgagc gtggcgcgac cggtcgcgtc cgcggcgcgc gcgacgatcc
      421 ccccgccgac gacggcgtca ccgtcgtaga agaccgccgc ctgaccgggc gccacgagcg
      481 cgggcgcgtc gaggtcgagg aaggcgcggt cgtcctgcac gccgacccat cctgcgagcg
      541 gcgtgccgcg gtacctgagg accgcctcgg tccggaatcg ctccgccggc gtcgcgtcgc
      601 cggtgaagcg cacgtcccgc agctcgtagc acgtcgcgct gatctcttcg cgcgtaccga
      661 cgatcgccgc gcgacggtcg ggctcgaggc gcagcacgta catgcgctca ccactggcga
      721 ccccgacgcc ggatcgctgt ccgatggtca atgtgccgat gccgccgtgt tcggcgacga
      781 cggtgccgtc gagccgttcg acgggcccgc gcacgaacgc tcccggtgcg cgctcgcgca
      841 cgaagccgcg atagtcgccg ccgatgaaac agatgtcctg gctgtcgggc ttgctggccg
      901 tcggcagccc gaagcgcgac gcgagcgcgc gcgtctcgct cttgtcggcg agctcaccga
      961 cgggcagctc gagccgggcc agctcgcgct ggccgagcat gtacagcgcg tacgactggt
     1021 ccttggccgc atcccgcgcg cggaggagcc gatgccgccc gccatgctcg acgcgcgcgt
     1081 agtggccggt cgcgagacga tcggcgccga acttcgcgac gacgtcgcgc atgagcgcct
     1141 cgaacttcac gtagtggttg caggcgacac agggattggg cgtctcgccc gccgcgtacg
     1201 aatccacgaa gcgatcgacg acctcgcggc cgaagagctc cgcgtagtcg agcgcgtaga
     1261 aggggatgcc gagcaccgcc gccgcgcggc gcgcgtcctc tcccgcctcg tcggtcccgc
     1321 agcagcgcgg cgcgtcgccg ctcgtcccgg cgggcgtgag gcgcatccag acgccgacga
     1381 cgtcctcgcc gcgctcggca aggagcgccg cggcgaccga cgagtcgacc ccgccgctca
     1441 tcgcgacgaa gacccgactc acgggagatc cttcacgcgc gcgtcgacga tcgcggcctg
     1501 cagcgcggag agcaccgtca gcgcgcagcg ctcgccgtcg gcatcgagac gcagcgtctc
     1561 gatgatgtcc cgcacctgga tacgcgcgat ctcgtcggcg atctcgccgc cgacccgctg
     1621 ccggatctcc tcggcggcgc ggttcgccgc ggcgcacgcg ttcagctccg tgcccacgat
     1681 gcgcacgcgc cggtcgacca cctcgaccgt gaacgtcacg cggtcgccgt cgcgcgggtt
     1741 cgcgtcgctg ccggtgcccg acgtcatgcc gtcacgatgg aggcgcctcg cacgcgcgcg
     1801 acgatcgcgg gcagcgccgc gatgaacgca tcgacgtccg cctcgcttgt gccgcggccg
     1861 acgctgacac gcagcgagcc cttcgcggtc gactcgtcga ggccgagcgc gagcagcacg
     1921 tgcgacggct cggtcgttcc cgacgtgcag gcggagccgc tcgagaccgc gaatccttcg
     1981 agatcgagcg ccatcaccag cgacggcccg tcggcgccgc ggaagcagaa cgaggcgttg
     2041 ttgggcagcc gctccgtccg gtgcccggtg agcacggcgt cgggcaccc
//
LOCUS       DASTZR010000199         2325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_34534, whole genome shotgun sequence.
ACCESSION   DASTZR010000199 DASTZR010000000
VERSION     DASTZR010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34534"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFV20_04430"
     CDS             complement(<1..183)
                     /locus_tag="VFV20_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEU5287627.1"
                     /translation="MKCVVVAHGEVAPRDRDEADGADLVIAADGGALALERWGIVPKL
                     VVGDLDSLGPEHAARLG"
     regulatory      complement(258..366)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(557..1786)
                     /locus_tag="VFV20_04435"
     CDS             complement(557..1786)
                     /locus_tag="VFV20_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEU5287628.1"
                     /translation="MDKINTGDTACLLASAALVMLMTPALGFFYGGLVRRKNVLATIM
                     HSFFILCLISVQWVLWGYSLAFGPDHGGLIGGLDWAALKGVGQEPNADYAATVPALAF
                     MVFQMMFAVITPALITGSFAERKRFKAFVVFTLLWATLVYDPVAHWVWGTGGWLRSLG
                     ALDFAGGTVVHITSGVSALVAALVLGRRRGYGMEPIEPHDATMTVLGASLLWFGWFGF
                     NAGSALTSGGLAANAFVVTNTAAAMSALTWMTVSWLHKGQPSVLGAAAGAVAGLVAIT
                     PASGYVDVSASILIGLGSGTLCYVGIQLTKRLKVDDALDVFGVHGVGGMWGAIATGIF
                     ATVAVNSAAADGAAYGNLGQLGIQGIAVLASVTYSAVMTFVILKVINLFVGLRVPEHE
                     EVLGLDASQHGEAAYQI"
     gene            1986..2171
                     /locus_tag="VFV20_04440"
     CDS             1986..2171
                     /locus_tag="VFV20_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287629.1"
                     /translation="MTAVVQWRRPGVPVRRVHLSLPEPPRSRRISRWAREPKDRLART
                     LEDWLSDLAVATTQDAD"
BASE COUNT          400 a          804 c          815 g          306 t
ORIGIN      
        1 cccgaggcgc gcggcgtgct ccgggcccag cgagtccagg tcgccgacca cgagcttcgg
       61 cacgatgccc catcgctcga gtgcgagcgc gccgccatcc gcggcgatca ccagatccgc
      121 cccgtccgct tcatcgcggt cgcgcggagc gacctcgccg tgcgcgacga cgacgcactt
      181 catggcgacg ctggagcggc ggcgccgctc cgtgaccggc tgaacaaaca aatgcggcgc
      241 acccgagcgc cgcagcgagg tcgcatgagt cgactcccta cgctgacatt actcagatca
      301 ggttctgggg tctgcgttca ccgcactctc agcgccccgc ttccgggcgc tcccccgtcg
      361 tatgcagttg tcgaaagaaa cactagcacc gcggccgccc tgaggtcgcg gccgcggtgc
      421 atcgtgaccg aacgtacggc taggccgcgg cgcgctcctc ggtgaccacc tcgatcttcc
      481 gcgacggcgc gaagagccac gcgatgatca ggagcgcgaa cgacgcgaat gcgaggaact
      541 ccatctgcgt cctcctttag atctggtacg cggcctcgcc gtgctgcgac gcgtcgagcc
      601 cgagcacttc ctcgtgctcc ggaacgcgca gcccgacgaa gaggttgatg accttcagga
      661 tcacgaacgt catgaccgcg gagtaggtga ccgacgcgag caccgcgatg ccctggatgc
      721 cgagctgccc gaggttgccg tacgcggcgc cgtcggccgc ggccgagttc accgcgacgg
      781 tcgcgaagat gccggtcgcg atcgcgcccc acatgccgcc gacaccgtgg acgccgaaca
      841 cgtcgagcgc gtcgtcgacc ttgagacgct tggtgagctg gatgccgacg tagcagagcg
      901 tgcccgagcc gaggccgatc aggatcgacg cggagacgtc gacgtagccg gaggccggcg
      961 tgatcgcgac gaggccggcg acggcgcccg cggccgcacc gaggacgctc ggctggccct
     1021 tgtgcagcca cgagacggtc atccaggtga gggcggacat cgccgcggcg gtgttggtga
     1081 cgacgaacgc gttcgcggcg agcccgcccg aggtgagcgc gctgccggcg ttgaagccga
     1141 accagccgaa ccacaggagg ctcgcgccca ggacggtcat cgtggcgtcg tgcggctcga
     1201 tcggctccat gccgtagccg cggcgccggc cgagcaccag cgcggcgacg agcgccgaaa
     1261 cgccgctggt gatgtggacg acggtgccgc cggcgaagtc gagcgcgccg agcgaccgca
     1321 gccagccacc cgtgccccag acccagtgcg cgaccgggtc gtacacgagg gtcgcccaga
     1381 ggagcgtgaa cacgacgaag gccttgaaac gcttgcgctc cgcgaacgag cccgtgatga
     1441 gggcgggcgt gatgaccgcg aacatcatct ggaacaccat gaacgcgagc gcggggacgg
     1501 tggccgcgta atcggcgttc ggctcctgtc cgacgccctt cagtgccgcc cagtcgagac
     1561 cgccgatgag cccgccgtgg tcggggccga acgccagcga gtagccccag agcacccact
     1621 ggacgctgat caggcagagg atgaagaagc tgtgcatgat cgtcgcgaga acgttcttcc
     1681 gtcggacgag accgccgtag aagaagccga gggccggcgt catgagcatg acgagtgcgg
     1741 cggacgcgag gagacacgct gtgtcgcccg tgttgatctt gtccatgcgt gacaggctgc
     1801 gcatccggcc gacgtcggtc agcggcggat gggcaccgcg aaggttgcgg ttcggtcctg
     1861 ggcatgaggg ggaattcggt gacggcgctc gccaccgttc cggcaccgaa gcgccaccgt
     1921 ccggccgttc accccggccc gccggctgcc gatgctatgg accagtcagc gctcgaggag
     1981 aggacatgac cgcggtcgtc caatggcgcc gccccggcgt tcccgtccga cgcgtgcacc
     2041 tgtcgctccc cgagccgcca cgctcgcggc gcatctcgcg ctgggcgcgc gaaccgaagg
     2101 accggctcgc acggaccctg gaggactggc tcagcgacct cgcggtcgcg acgacccaag
     2161 acgcggacta gacacgcccg ctcgaggcgg atgagcgcag tggcagcggg cgcgatggcg
     2221 cgtaccgtct gtacacggcg aacgacagcg tggcgatgac cagcccgaac gtgaagaaga
     2281 atggtgccgt ccaagccacc acggcgtcgg gcgtctcgag cacgg
//
LOCUS       DASTZR010000200         2639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_974147, whole genome shotgun sequence.
ACCESSION   DASTZR010000200 DASTZR010000000
VERSION     DASTZR010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974147"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2506
                     /locus_tag="VFV20_04445"
     CDS             <1..2506
                     /locus_tag="VFV20_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287630.1"
                     /translation="RELTKELEVTFGEASGGWRELARDTDRQRQLFDGKTLREALATT
                     DGSRGDPFPTRHKTAFKALVRFMYEAGSWVSLKNELFRTDPELAKALFEVRQAWAEWI
                     TSDAGNRATVIGTVKNMTNDCDFNFALGEGATLDPVPRMRKVAEETQRLVREALRETG
                     LSEADAAKADYQILFESNSYTDPAILHLYTRLPEAAREQAVRLLTRDSVGVSRWMIRE
                     GFEHLPLDVRIRAVRDLDLWATAGVRTPEEIARDLGFTGTHWHTTEGREWLLAEQSRL
                     FERYLADPTNVEVVTKICETQMLLNTMAEGAYVTPGAGLVTVTIKEGLYTKDMTMVRR
                     QMNELKRELAERDDATKRKLLETLEKEYEQQRIETIKSLTPDEALQSVLGDLVFFWHM
                     AETASHDLEAFDIVMRYEFSKYVARLVEVAKAFGMDPASMTPGDHRFTGIAKLLRTAT
                     KIYKGKKPWEAFGEGPAFTSLTEMRQYFSSVQKAASDVVRYLLEHSSFRDRLDLKAES
                     FGPPATPDRSTSAREDAWLSLANMTIAERELRLILGGDASYAGREHVALDFFYDQYEA
                     NLARVRKGASEEGASYWSHFSSEIDVQYLQRRLEFWRTRGTPLETREALHTRLGEDAA
                     KTAVLEVPDFEGGKTAVLEIPDFDGSKTAVLEVPDLPATAPDLARLKKAAASQASALK
                     VTSVRTDSFAVVGDDLGTFLRGMRNLRPVAGYVDVFVHGGKDGFYILRDTSWVKVSAK
                     ELADALWAAGYDGRPIRLVSCETGAVDASNATLGAAADALAKILKTEIWAPNKTIWLG
                     HDGRLFISSREPATWTPQSKPSGVWRAFGSKPKGTP"
BASE COUNT          442 a          877 c          940 g          380 t
ORIGIN      
        1 gcgcgagctc acgaaggagc ttgaggtcac gttcggcgag gcgtccggtg gctggcgcga
       61 gctggcgcgc gacacggatc gccagcggca actcttcgac ggcaagacgc tgcgcgaggc
      121 gctggccacg acggacggct cgcggggtga tccgttcccg acgcgccaca agaccgcgtt
      181 caaggcgctc gtgcgtttca tgtacgaggc ggggagctgg gtgagcctga agaacgagct
      241 cttccgtacg gatccggagc tcgcgaaggc actcttcgag gtgcggcagg cctgggccga
      301 gtggatcacc tccgatgccg ggaatcgcgc cacggtcatc ggcacggtga agaacatgac
      361 gaacgattgc gacttcaact tcgcgctcgg cgagggcgcc acgctcgatc ccgttccgcg
      421 catgcggaag gtcgccgagg agacgcagcg cctcgtgcgc gaggcgctcc gcgagaccgg
      481 cctgtcggag gcggacgcgg cgaaggccga ctaccagatc ctgttcgaga gcaactcata
      541 caccgacccc gcgatcctgc acctctacac gcggctgccc gaggccgcgc gcgagcaggc
      601 ggtacggctg ctcacgcgcg actcggtcgg cgtgtcgcgg tggatgatcc gcgagggttt
      661 cgagcatctg cccctcgacg tgcgcatccg tgccgtccgc gacctcgacc tgtgggcgac
      721 cgccggggtc cggacaccgg aggagatcgc gcgtgacctc ggcttcaccg gtacgcactg
      781 gcacaccacc gaagggcgcg agtggctgct cgcggagcag agccgactct tcgagcggta
      841 cctcgcggat ccgacgaacg tcgaggtcgt cacgaagatc tgcgagacgc agatgctcct
      901 gaacacgatg gcggagggcg cgtacgtcac gcccggcgcg ggactcgtca cggtgaccat
      961 caaggagggc ctgtacacga aggacatgac gatggtgcgc cgccagatga acgagctgaa
     1021 gcgcgagctg gcggagcgcg acgacgcgac gaaacggaag ttgctcgaga ccctcgagaa
     1081 ggagtacgag cagcagcgga tcgagacgat caagtcgctc acgcccgacg aggcgctcca
     1141 atcggtgctc ggcgacctcg tcttcttctg gcacatggcg gagacggcgt cgcacgacct
     1201 cgaggccttc gacatcgtca tgcggtacga gttctcgaag tacgtcgcgc gcctcgtgga
     1261 ggtcgcgaag gcgttcggca tggaccccgc gtcgatgaca cccggcgatc accggttcac
     1321 gggcatcgcc aagctcctgc ggacggcgac gaagatctac aagggcaaga agccgtggga
     1381 ggcgttcggc gagggcccgg ccttcacgtc gctcacggag atgcggcagt acttcagctc
     1441 ggttcagaag gccgcgagcg acgtcgtgcg ctacctactg gagcacagct cgttccgcga
     1501 ccgcctcgac ctgaaggccg agtccttcgg tccgcccgcg acgcccgaca ggtcgacgag
     1561 cgcgcgcgag gacgcctggc tctcactcgc gaacatgacg atcgcggagc gcgagcttcg
     1621 cctcatcctc ggcggagacg cctcgtacgc cggccgcgag cacgtggccc tcgacttctt
     1681 ctacgaccag tacgaggcga acctcgcgcg tgtgcggaag ggtgcatccg aggagggggc
     1741 gtcgtactgg tcgcacttct cgagcgagat cgatgtgcag tacctgcagc ggcgcctgga
     1801 gttctggcgg acccgcggca cgccgctcga gacccgcgag gcgctgcaca cacgtctcgg
     1861 ggaggacgcc gcgaagaccg cggtgctcga ggtccccgac ttcgagggcg ggaagacggc
     1921 cgtcctcgag atccccgact tcgacggctc gaagaccgcc gtgctcgagg tgccggacct
     1981 gccggcgacc gcgccggacc tcgcgcggct gaagaaggcc gcggcctcgc aggcgtcggc
     2041 cctgaaggtg acgagcgtgc gtacggactc gttcgcggtc gtcggtgacg atctcgggac
     2101 gttcctccgc ggtatgcgga accttcgtcc cgtggccggg tacgtggacg tcttcgtcca
     2161 tggcggcaag gacggcttct acatcctgcg cgacacgtcc tgggtgaagg tgagcgcgaa
     2221 ggagctcgcc gacgcgctgt gggccgcggg ctatgacggc cgacccatcc gtctcgtgtc
     2281 ctgcgagacg ggtgcggtcg acgcgtcgaa cgcgacgctc ggcgccgccg ccgacgccct
     2341 cgcgaagatc ctcaagacgg agatctgggc gccgaacaag acgatctggc tcggccacga
     2401 cgggcggctg ttcatctcct cgcgcgagcc cgcgacgtgg acgccgcaga gcaagccgtc
     2461 cggcgtctgg cgggcgttcg gctcgaagcc gaagggcacg ccgtgacgct gcgcgaggcc
     2521 gggttcgagc cgatcgagcc gggggaccac gcgtggctcg acgaggagcg ggccgcgacg
     2581 tacctcgagt ccgcgccggt cctcgcggtc tcaccccagg tggccggctc cgccgcgct
//
LOCUS       DASTZR010000201         4222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_142134, whole genome shotgun sequence.
ACCESSION   DASTZR010000201 DASTZR010000000
VERSION     DASTZR010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142134"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1628)
                     /locus_tag="VFV20_04450"
     CDS             complement(<1..1628)
                     /locus_tag="VFV20_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU5287631.1"
                     /translation="MTACLVVGIVVTPADSAAPTRNRGYWIAGANAQVFAFGDAQLYA
                     QRVRRQVRGQIADIAAHPSGRGYWLLGRDGAVYAYGEARDLGQDSAREQDAVAIASTP
                     TGNGYFVVTERGEVHGFGDAVARGSRSARERGEERKIVDIATTPSGGGYWLVDANGQV
                     SAFGDAIGFGSLPRTGHDEEDDRSGDDRSDIVAIGATARGDGYWLASASGQVFAFGAA
                     PRFAPLADPARGRHDEEDNERKAKKIVDLTTTWTGRGYWLVDRDGLVFAFGDAALYGE
                     LARRDLRSGQVVAIVSTPFVNHDPVAVNDTAALDEDTTVDINVLANDTDEDGDPLTGA
                     VLTQPAHGTATLNPDGTIRYAPAHDYNGSDAFTYRVTDGFGGSATGTVRLTVRPVNDA
                     PIAFDDAFTIDEDTALSDSVTKNDTDVDGDALTGRLVTAPAHGVATLGADGTFTYTPA
                     ANYNGDDSFTYRAFDGVLESGTAATVYIHINAVNDAPVGAADTATVNEDEVLTVAPPG
                     VLANDTDVESDPLTVSLVSGPAHGTLSLTPNGGYT"
     gene            complement(1731..2474)
                     /gene="tssD"
                     /locus_tag="VFV20_04455"
     CDS             complement(1731..2474)
                     /gene="tssD"
                     /locus_tag="VFV20_04455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HEU5287632.1"
                     /translation="MDNARPDRDALEAELAELRARLKTQGDELTALRAQLAPSNGPTR
                     RDLLRAGGLVGAGLAALTVAGVAGATPQPAAAIEGHNLRLRGGLLINLYMKGQKSGDI
                     KGSVTQKGREGSISVQYLQQKRVSPRDAASGLPTGKIQHEPIVFRKTIDKSTPILLNV
                     MSTNENITNASFKFWKPSATGVETQYFTIELQNASIASYDLYHPDSTDSGASAATAEL
                     EEISLTYQKIIWTWNDGGITAQDDWGGLA"
     gene            2557..4035
                     /locus_tag="VFV20_04460"
     CDS             2557..4035
                     /locus_tag="VFV20_04460"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEU5287633.1"
                     /translation="MPFGELSRLAAITPERAKTIRARVLAARYGEREPLPPETTSTTL
                     MPEELRVPLVRWSLERDTHPAAGLALLFQHLLPVRRAQVERALGADAIGWLVDAGLIA
                     ERDGTLEPRFRLVVADDVFIWCDEANAGPDAVMTPGPTTADLIALIPTPVGGSVLDIG
                     TGPGTLALVAARRGATRVVATDISERAIALTRFNAAFNEIPLEVRLGDLYAPVSTERF
                     DWIMAQPPYVTHPADEPGVTFLHGGAMGDELAMAVLEGVDAHLRKRGVAMVLFDSPVR
                     PDASLEARVRGAVGPNVDVATFSQPGLHPDRQALGYASLADPTFGRRYEETAVRYRAH
                     LHAKRISEVTHSLVVVRAPDRPEHSAWTAALTVARFPEEWSELSGFMRGIDLAATGED
                     ALATARVRPRPGAMIVIEREPGAARDRETRSIRFATANIALDRELTQAGGVIFDLLAA
                     ESSVASAIDRFAAAMERPPSEVRPLVTSFVRDCLVRALLVPD"
BASE COUNT          571 a         1454 c         1496 g          701 t
ORIGIN      
        1 gtgtagccgc cgttcggagt gagcgagagc gtgccgtgcg ccgggccgct cacgagggac
       61 acggtgagcg ggtcgctctc gacatcggtg tcgttcgcga gcacgccggg cggagcgacg
      121 gtcaggacct cgtcctcgtt gaccgtggcg gtgtccgccg cgcccaccgg ggcgtcgttc
      181 accgcgttga tgtggatgta caccgtggcg gcggtgccgg actcgagcac accgtcgaac
      241 gcgcgatagg tgaacgagtc gtcgccgttg tagttggcgg ccggcgtgta ggtgaacgtc
      301 ccgtccgcgc cgagggtcgc cacaccgtgg gccggcgcgg tcaccaggcg gccggtgagg
      361 gcgtcgccgt cgacgtccgt gtcgttcttc gtcacggaat ccgagagcgc ggtgtcctcg
      421 tcgatcgtga acgcgtcgtc gaacgcgatc ggcgcgtcgt tgaccgggcg cacggtgagc
      481 cggaccgtgc cggtggccga gccgccgaat ccgtcggtga cgcgataggt gaacgcgtcc
      541 gagccgttgt agtcgtgcgc gggcgcgtag cggatcgtgc cgtcgggatt gagcgtcgcg
      601 gtgccgtgcg ccggctgcgt gaggaccgcg ccggtcagag gatcgccgtc ctcatcggtg
      661 tcattggcga gcacgttgat atcgacggtc gtgtcctcgt cgagcgcggc cgtgtcgttc
      721 accgccaccg gatcgtggtt gacgaatggc gtcgaaacga tggccaccac ctggccgctg
      781 cgcaggtcgc gccgcgcgag ctctccgtag agggccgcgt cgccgaaggc gaagacgagg
      841 ccatcgcggt cgaccagcca gtacccgcgt ccggtccacg tggtcgtcag atcgacgatc
      901 ttcttggcct tccgctcgtt gtcttcctcg tcgtgccgtc cgcgcgcggg gtcggcaagc
      961 ggcgcgaacc ggggtgcggc tccgaaggcg aacacctggc cgctcgcgct cgcgagccag
     1021 tagccgtcgc cgcgagcggt cgctccgatc gcgacgatgt cgctgcggtc gtcgcccgag
     1081 cggtcgtcct cctcgtcgtg gccggtccgc ggcagcgagc cgaagccgat cgcgtcgccg
     1141 aaggccgaga cctgaccgtt cgcgtcgacc agccagtagc cgccgccgga cggcgtcgtc
     1201 gcgatgtcga cgatcttccg ctcttcgccg cgttcgcgcg cgctgcgcga tccgcgggcg
     1261 accgcgtcac cgaagccgtg gacctcgccg cgctcggtga ccacgaagta gccgttgcct
     1321 gtcggggtcg aggcgattgc gacggcatcc tgctcgcgcg cgctgtcctg gccgaggtcg
     1381 cgtgcctcgc cgtaggcgta gacggcgccg tcacgaccga gcagccagta gcccctcccc
     1441 gagggatggg cggcgatgtc cgcgatctgg ccgcggacct ggcggcggac gcgctgcgcg
     1501 taaagctgcg cgtcgccgaa ggcgaagacc tgcgcgttgg cgccggcgat ccagtaaccg
     1561 cggttccggg tgggcgcggc cgagtcggcc ggcgtcacga cgatgccgac gacgagacag
     1621 gcggtcacga tggccgccat gcccctgacc catgcgcgcg taccgagctt cagttcagtg
     1681 cctcaccctt cccgccgttg cgtcgtagtg gacgtgcgcg aaaggtgcgg ctaggccagc
     1741 ccgccccagt cgtcctgggc cgtgatcccg ccgtcattcc acgtccagat gatcttctgg
     1801 taggtgagcg agatctcttc gagttcggcg gtcgccgcgc tcgcgcccga gtccgtgctg
     1861 tccgggtgat agaggtcata gctcgcgatc gaggcgttct gcagctcgat cgtgaagtac
     1921 tgcgtctcca cgcccgtcgc ggacggcttc cagaacttga acgacgcgtt ggtgatgttc
     1981 tcgttcgtcg acatgacgtt gaggagaatg ggggtgctct tgtcgatggt cttgcggaac
     2041 acgatcggct cgtgctggat cttcccggtc gggagtcccg aagcggcgtc gcgcggactc
     2101 acgcgcttct gctgcaggta ctggactgag atcgaaccct cgcggccctt ctgcgtcacg
     2161 cttcccttga tgtcgcccga cttctgcccc ttcatgtaga ggttgatgag caggccacca
     2221 cgcagccgga ggttgtggcc ttcgatcgcc gcggcgggct gtggtgtcgc gcccgcgacc
     2281 ccggcgaccg tgagtgccgc gaggcccgcg cccacgagcc caccggcgcg cagcaggtcg
     2341 cgtcgtgtcg gtccattcga cggagcgagc tgcgcgcgca gcgcggtgag ctcgtctccc
     2401 tgcgtcttga gcctggctcg cagctcggcg agctcggcct cgagcgcgtc gcgatccgga
     2461 cgtgcgttat ccatgaatcc cctccaccac ttgtccccaa cgccgcggct aaggtagcgc
     2521 atggcggctt ctccgccaag cgagaaagga gcgatcgtgc cattcggtga gctttcgcgc
     2581 ctcgcggcga tcacgcccga gcgcgcgaag acgatcaggg cgcgggtgct cgcggcacgc
     2641 tatggagagc gggagccgct gcctcccgag acgacgtcca cgacgctcat gccggaggag
     2701 ctgcgggtcc cactggtgcg ctggtcactc gaacgggata cccaccccgc cgccggtctc
     2761 gcgctgctat tccaacacct cctgcctgtc cgtcgtgcgc aggtcgagcg cgcgctcggt
     2821 gcggacgcga tcggatggct ggtcgacgcg ggtctcatcg ctgaacgtga tgggaccctg
     2881 gagccacgct tccggctggt ggtcgccgac gacgtcttca tctggtgcga cgaggcgaat
     2941 gcgggaccgg acgcggtcat gacgcccgga ccgacgaccg cggatctgat cgcgctcatc
     3001 ccgacaccgg tcggcggcag cgtcctcgat atcggcaccg gcccgggcac cctcgcgctc
     3061 gtcgccgcga ggcgcggtgc gacccgcgtc gtcgcgaccg acatcagcga gcgcgcgatc
     3121 gcgctcaccc gattcaacgc ggccttcaac gagatccctc tggaggtgcg gctcggcgac
     3181 ctctacgcgc cggtctcgac ggagcgcttc gactggatca tggcgcagcc gccatacgtc
     3241 acacatccgg ccgatgagcc cggggtcacg ttcctccatg gcggtgcgat gggtgacgag
     3301 ctcgcgatgg ccgtcctcga aggcgtcgat gctcatctgc ggaaacgagg ggtcgcgatg
     3361 gtcctgttcg attcgccggt gcgcccggac gcgagcctcg aagcgcgcgt gcgtggcgcg
     3421 gtgggcccga acgtggacgt cgcgacgttc agccagcccg gtctgcaccc ggaccgccaa
     3481 gctctcggct acgcgtcgct cgcggacccg acgttcggac ggcgctacga ggagaccgcc
     3541 gtgcgctacc gcgcgcacct gcacgcgaag cgcatcagtg aggtgaccca ctcgctcgtg
     3601 gtcgtgcgcg cgccggatcg tccggagcac tcggcatgga cggcggccct cacggtggcg
     3661 cgattccccg aggaatggtc cgagctgagc ggtttcatgc gaggcatcga cctcgccgcc
     3721 accggcgagg acgcgctcgc cacggcacgt gtccgtcccc ggcccggcgc gatgatcgtg
     3781 atcgagcgcg agcccggcgc cgcgcgcgac cgggagacgc gctcgatccg gttcgccacg
     3841 gcgaacatcg cgctcgatcg cgagctcaca caggcgggtg gcgtgatctt cgatctgctc
     3901 gcggccgagt cgtcggtggc atccgcgatc gaccgcttcg ccgcggcgat ggagcgaccg
     3961 ccatcggagg tgcggccgct cgtgacctcg ttcgtgcgcg actgtctggt acgcgcgctc
     4021 ctcgttccgg actgagccgc gccggaacag cacgcggacc tgccgccgcg cgcgctcgcc
     4081 tagactccac cgttctccca tggaaaaggt cgtctggatg ttccccggtc agggctcgca
     4141 ggtcgtcggt atgggccgcg agctggccga gcgccacgcg agcgcgctgc acgtcttcga
     4201 cgaggcatcg cacgcgctcg gg
//
LOCUS       DASTZR010000202         3369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1134234, whole genome shotgun sequence.
ACCESSION   DASTZR010000202 DASTZR010000000
VERSION     DASTZR010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134234"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VFV20_04465"
     CDS             complement(<1..297)
                     /locus_tag="VFV20_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287634.1"
                     /translation="MRYAIALLLGSLAGLIAAFQPIADTDMWWHLATGRETLAHGFVR
                     ADVFSWTVRGAPVSTDQWLGQVVMYATWLATGWRGIAVLRIVLVTLLVAFVALGA"
     gene            complement(294..1670)
                     /locus_tag="VFV20_04470"
     CDS             complement(294..1670)
                     /locus_tag="VFV20_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287635.1"
                     /translation="MTRIPAFALLAGAFFGALVSLQPIGDSDLFWHVEAGRRTLAGAL
                     PRADVFSWTVAGAPVFTDQWLGDVILAAARTIGDWRGVLALRVVAVAVLVAVIVATAM
                     AARPGRPIVAVAAALPAIALSRFAWTDRPELLGLVCFAILVPLLRGGDRGLLVSIPLL
                     AIWTQLHGSFAIGLALVLATCAARGLTERRDRWRFVPIASAAAVATLLGPSGVATWTS
                     SGGHFLAPPRYIAEEGPPDTSSLAGVLFFVTLGLLLASALLSRRRATLEALAILLPVA
                     FVSLTAARHMPLLAIAAAPFLAERLPDPLATWRERASRGGGARATVSARSGHAVVAAA
                     SAAALALAILIADGRVDETGYPRAALAALPSGPGLLNRYEWGGWLMYAAPRTPVFIDG
                     RLFPYVPGVLDDYRAILNAHPGWEAVADRRGVRTMLVRPDDPIAGRAPDRGWRVAYRD
                     DVALVLAR"
     gene            complement(1667..3025)
                     /locus_tag="VFV20_04475"
     CDS             complement(1667..3025)
                     /locus_tag="VFV20_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287636.1"
                     /translation="MTGRRAAPLLVALGAFALAVARPSSDPDMFWHLASGRWMVEHRD
                     LLRVDQFSSTVSGARYSVGEWLGEIVLYGAYAAAGWPGLALLRGALVAVAAFFVTRSA
                     VGTGARLLIAVPLATLAMLLSTIVWTDRPHLFTLALFPLLLDLLLRARTGDLRPLIAV
                     PPLLLVWTDLHGGYALGLALVMLFAVEAAVTRRRVVPFTAAAVLAAAATLLDPGSLGI
                     GAAFAHAAAPPRFIVEETPPDVLTAPGFLFAFFVLGTLGVAMRAGGTLIDALVIVPLL
                     WLGLSAQRDMPYFAMAAVPFLARQAAAAWSTLERVPRAHAPRGTLAGLAVGTVAIAVL
                     NLTSLPGAPDERAYPSGALAELRQGTGVLLNEYDWGGYLIWRAPERRVFIDGRLFPYL
                     PTVFADWNAAVRLAPRWREVLESYRVNAVLLRPDRPLVGALRESGWRETAGREWVLLT
                     RP"
     gene            complement(3022..>3369)
                     /locus_tag="VFV20_04480"
     CDS             complement(3022..>3369)
                     /locus_tag="VFV20_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010888163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287637.1"
                     /translation="ALAVAQWDASFNAGTRVREAALDAIPAGSFLAIDAAAWRWISGR
                     TVAVTPADGLARAACVARAIDARSVVLEAAHFSRYERLYSDDERPVWLGAPTVRGATK
                     IYPIRGEPPCDAR"
BASE COUNT          570 a         1199 c         1279 g          321 t
ORIGIN      
        1 cgcgccgagc gcgacgaacg cgacgagcag ggtgaccagc acgatgcgga gcaccgctat
       61 gccgcgccag ccggtggcga gccacgtggc gtacatcacg acctggccga gccactggtc
      121 ggtggagacg ggggcaccgc ggaccgtcca cgagaacacg tcggcgcgca cgaacccgtg
      181 cgcgagcgtc tcgcgccccg tggcgagatg ccaccacatg tccgtgtcgg cgatcggctg
      241 gaaggcggcg atcagacccg cgagcgatcc gaggaggagg gcgatggcgt atctcatcgt
      301 gcgagcacga gcgcgacatc gtcgcgatac gcgacgcgcc agccgcggtc gggtgcgcgg
      361 ccggcgatcg gatcatcggg ccgcacgagc atcgtgcgca cgccgcgccg gtccgcgacg
      421 gcctcccaac ccggatgcgc gttcaggatc gcgcggtagt cgtcgagcac gcccgggacg
      481 tacgggaaga gacggccgtc gatgaagacc ggcgtccggg gtgccgcgta catcagccat
      541 ccgccccatt cgtagcggtt cagcaggccc gggcctgacg gcaacgcggc gagcgccgca
      601 cgcggatagc cggtctcatc gacacggccg tctgcgatca ggatcgcgag cgccagcgcg
      661 gcggcgctcg ccgcggccac aacggcatga ccggaccgtg cgcttaccgt cgcccgagcg
      721 cctccgcctc gcgacgcgcg ctcgcgccac gtcgccagcg gatcgggcag ccgctcggcg
      781 aggaacggcg ccgcggcgat cgcgagcagc ggcatgtgtc gggccgccgt gagcgacacg
      841 aacgcgacgg gcaggaggat cgcgagcgct tccagcgtgg cgcggcggcg cgacagcagc
      901 gcgctcgcga gcagcagccc gagcgtcacg aagaacagca cacccgcgag gctgctcgta
      961 tcgggcggac cctcttcggc gatgtatcgc ggcggggcga ggaagtgtcc gcccgacgat
     1021 gtccacgtcg ccacgcccga cgggccgagc agcgtcgcga ccgcggcggc cgatgcgatc
     1081 gggacgaagc gccagcggtc gcggcgctcg gtgagaccgc gcgccgcgca ggtcgcgagc
     1141 acgagagcga ggccgatcgc gaacgagcca tgcagctggg tccagatggc gaggagcggg
     1201 atcgagacga gcaggccgcg gtcccctccc cggagcagag gcacgaggat cgcgaagcac
     1261 accaggccga gcagctccgg gcgatcggtc cacgcgaagc gtgagagcgc gatcgcgggg
     1321 agcgcggcgg ccacggcgac gatcggccgg cccggccgcg cggccatcgc cgtcgcgacg
     1381 atcacggcga cgagcaccgc gaccgcgacg acgcgcagcg cgagcactcc ccgccaatcg
     1441 ccgatcgtcc gagcagcggc gaggatcacg tcgccgagcc attgatcggt gaagaccggc
     1501 gcgccggcga ccgtccacga gaacacgtcg gcccgcggaa gcgcccccgc gagcgtccgg
     1561 cgcccggcct ccacgtgcca gaacaggtcg ctgtcgccga tcggctggag gctcacgagc
     1621 gcgccgaaga aggcgccggc gaggagcgcg aacgcgggga tgcgcgtcac ggccgcgtca
     1681 gcaggaccca ttcgcggccc gccgtctccc gccaaccgga ctcgcgcagc gcaccgacca
     1741 gcggccggtc cggccggagc agcaccgcat tcacgcggta ggactcgagc acctcgcgcc
     1801 atcgcggagc gagacgcacg gccgcgttcc agtcggcgaa caccgtcggg agatacggga
     1861 acagccggcc gtcgatgaag acgcgccgct ccggggcgcg ccagatgagg tacccacccc
     1921 agtcgtactc gttgagcagg acgccggtcc cctgccgcag ctcagcgagc gcgcccgatg
     1981 gataggcacg ctcgtcgggt gccccgggga gtgatgtcag attcaggacc gcgatggcca
     2041 cggtcccgac cgccaggccg gcgagcgtcc ctcgcggcgc gtgcgcacga gggacacgct
     2101 ccagagtcga ccacgcggcg gcggcttggc gcgcgaggaa cggcaccgcg gccatcgcga
     2161 agtacggcat gtcgcgctgg gcggagaggc cgagccagag cagcggcacg atgacgagcg
     2221 catcgatgag cgttccgccg gcgcgcatcg cgacaccgag cgtgccgagg acgaagaagg
     2281 cgaacaggaa gcccggcgcg gtcaacacgt ccggcggcgt ctcctcgacg atgaagcgcg
     2341 gcggggccgc ggcgtgcgcg aacgcggcac cgatgccgag cgacccgggg tcgagcagcg
     2401 tcgccgcggc ggcgaggacg gccgccgccg tgaatggcac gacgcgccgc cgcgtcacgg
     2461 ccgcctcgac cgcgaacagc atcaccagcg cgaggccgag ggcatagccg ccgtggaggt
     2521 cggtccacac gaggagcagc ggcggtacgg cgatgagcgg acgcagatcg ccggtccgtg
     2581 cgcgcagcag gagatcgagg agcagcggga agagcgcgag cgtgaagagg tgcggccgat
     2641 cggtccacac gatcgtcgac aggagcatcg cgagcgtggc gagcgggacg gcgatcagca
     2701 gccgtgcgcc ggtgccgacc gccgaccgcg tgacgaagaa cgccgcgacg gcgacgagcg
     2761 caccgcgcag cagggcaagg ccgggccagc cggccgccgc gtacgcgccg tagaggacga
     2821 tctcgccgag ccactcgccg acgctgtacc gagcaccgct cacggtgctg gagaactggt
     2881 cgacgcggag gaggtcgcga tgctcgacca tccaccggcc ggacgcgagg tgccagaaca
     2941 tgtccggatc ggaggacgga cgcgcgaccg cgagcgcgaa cgcgccgagg gcgacgagga
     3001 gcggagccgc gcgccgtccg gtcatcgcgc gtcgcacggc ggctcgccgc ggatcgggta
     3061 gatcttcgtc gcgccgcgca ccgtcggagc gcccagccag accgggcgct cgtcgtcgga
     3121 gtacagccgc tcgtagcgac tgaagtgcgc ggcttcgagc acgacggagc gcgcgtcgat
     3181 cgcgcgcgcg acgcaggccg cgcgcgcgag gccgtcggct ggggtgaccg cgacggtccg
     3241 gccgctgatc caccgccacg cggccgcgtc gatcgcgagg aacgatccgg cggggatcgc
     3301 atcgagcgcg gcctcgcgca cgcgcgtccc cgcgttgaag ctcgcatccc actgcgcgac
     3361 cgcgagcgc
//
LOCUS       DASTZR010000203         2900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_11714, whole genome shotgun sequence.
ACCESSION   DASTZR010000203 DASTZR010000000
VERSION     DASTZR010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11714"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1030)
                     /locus_tag="VFV20_04485"
     CDS             complement(131..1030)
                     /locus_tag="VFV20_04485"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU5287638.1"
                     /translation="MNVSAELLEQYILTGLMIGVIYIVMALGITFIYSIMKMTNWAMG
                     EFYMVGSYVQYLVVSRILGADLWFLAVPIAAITVFFLGMAVQRILIRPMFERGMERRN
                     EYATIVTIALALFARGIVQVWAGPFERTPGSNMPSFQLGPLPIAGDRVAACIGGIVAL
                     AVFWFVIRRTWVGLALRAAAQSRAGVQTAGVDILRLDQVAFGIGVALAAVAGALLASV
                     YLVYPLNGMVTTTKGFEVVIIGGLGSIPGAIVGGIAVGLAEALGAALLATNYQNAYGF
                     VLVLIILLLRPRTGLFGETTRVA"
     gene            complement(1106..2329)
                     /locus_tag="VFV20_04490"
     CDS             complement(1106..2329)
                     /locus_tag="VFV20_04490"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5287639.1"
                     /translation="MVVGAFIVGACGGGAATGPLSASQAPSAAATTAASATRGPDVAI
                     GLQTPLSAPGAVTLGQQIQRGAELGVEYVNTVMGGVLGGRKLVMYTEDTQGQPETAVA
                     GYRRLVTEKKVAATFGYVHSSALLAVNEVAKDLGVPTISSQASAADITKKGYPIAFRT
                     HLIDPMRASAWLQLIKQKGWKKIAIIAEDTDYGVGLVNETKTQSQAMGLNLDLQVNVV
                     DRTAVDMTPQLLKMKDFKPDLILEIASAKQKDQIIDQAYQIGLYPAVPMLNSNDEPTA
                     TNFWDNHPKSGVGLMTIATYSPKQPLSDAAQWMADKYKAKYNEAAIYASLNGFGDVII
                     LAQALNNAKSTDSKALISALETGSFKGWTNTPVTFPKAEGVYWHNWSPPVLILQFTAE
                     KQDWREAPILISASK"
     gene            complement(2542..>2900)
                     /locus_tag="VFV20_04495"
     CDS             complement(2542..>2900)
                     /locus_tag="VFV20_04495"
                     /inference="COORDINATES: protein motif:HMM:NF016230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU5287640.1"
                     /translation="ALAFRFLPEVEQGHAIVDALWRDERPTRRVERSYPAPAEPTLLV
                     CRCEEVPLERVAAAFEEGASAIAGLKKRTRACMGRCQGRVCEDVLRRIAVPRGQPDLL
                     RHRPRVPARPIRMEDF"
BASE COUNT          480 a          955 c          968 g          497 t
ORIGIN      
        1 cccggcgccg ttcgggccga tgaggccgcg gatctcgccg cgcgcgatgc gcagcgtgat
       61 cccctggttc gcgcggacac ctccgaacgt gcgcgagatg tcgatcgcct ccaggagcca
      121 gggctgcgtg tcaggcgaca cgcgtcgtct cgccgaagag tcctgtccgc ggtcggagca
      181 ggagaatgat gagcacgagt acgaagccgt acgcgttctg gtagttcgtg gcgaggagag
      241 cggcgccgag cgcctccgcg agtccgaccg cgatgccgcc aacgatcgca ccggggatcg
      301 agccgagacc gccgatgatg accacctcga agcccttcgt cgtcgtcacc atgccgttca
      361 gcgggtagac gaggtagacg gaggcgagga gcgccccggc gaccgcggcg agcgcgaccc
      421 cgatgccgaa cgcgacctgg tcgagccgca ggatgtcgac gccggcggtc tgcacgcccg
      481 cgcggctctg tgcggccgcg cgcagggcga gcccgaccca ggtccggcgg atcacgaacc
      541 agaacaccgc aagcgccacg atcccgccga tgcaggccgc gacccggtcg ccggcgatgg
      601 ggagcggtcc gagctgaaag ctcggcatgt tggatccggg ggtgcgctcg aatggtccag
      661 cccacacctg cacgatgccg cgggcaaaca gcgcgagcgc gatggtcacg atcgtcgcgt
      721 attcgttgcg tcgctccatg ccgcgctcga acatgggacg gatgaggatc cgctgcacgg
      781 ccatgccgag gaagaacacg gtgatcgcgg cgatcggtac cgcgaggaac cagaggtcgg
      841 cgccgaggat ccgcgagacg accagatact ggacgtagct gccgaccatg tagaactcac
      901 ccatcgccca gttcgtcatc ttcatgatcg agtagatgaa ggtgatgccg agcgccatga
      961 cgatgtagat caccccgatc atgagaccgg tcaggatgta ctgctcgagc agctccgctg
     1021 atacgttcat ctctcgctgt tcttacctct agacacacca gccggcgggc acaagcccgc
     1081 cggctggtcg aacgtcatgc gtcgattact tcgaggcgga gatgaggatc ggcgcttcgc
     1141 gccagtcctg cttctcagcc gtgaactgga ggatgagcac gggcggcgac cagttgtgcc
     1201 aatagacccc ttccgccttc gggaacgtga cgggcgtgtt cgtccatccc ttgaacgatc
     1261 cggtctcgag ggcgctgatg agcgcctttg agtcggtcga cttcgcgttg ttcagcgcct
     1321 gcgcgaggat gatcacgtcg ccgaacccgt tcagcgacgc gtagatcgca gcctcgttgt
     1381 acttcgcctt gtacttgtcg gccatccact gggccgcatc ggacagcggt tgcttcggcg
     1441 agtacgtcgc gatcgtcatg agaccgacgc cgctcttcgg gtggttatcc cagaagttcg
     1501 tcgcggtcgg ctcgtcgttg gagttgagca tcggcaccgc cgggtacagt ccgatctggt
     1561 aagcctgatc gatgatctgg tccttctgct tcgccgacgc gatctcgagg atgaggtcag
     1621 gcttgaagtc cttcatcttg agcagctgcg gcgtcatgtc gacggccgtg cggtcaacga
     1681 cattgacctg cagatcgagg ttcaggccca tggcctgcga ctgcgtcttc gtctcgttga
     1741 cgaggccgac gccatagtcg gtgtcctcgg cgatgatggc gatcttcttc cagcccttct
     1801 gcttgatgag ctgaagccac gcgctcgccc gcatcgggtc gatcaggtgc gtgcggaagg
     1861 cgatcgggta gcccttcttg gtgatatcgg ccgccgacgc ctggctcgag atcgtcggga
     1921 cgcccaggtc cttcgcgacc tcgttcaccg cgaggagcgc ggagctgtgg acataaccga
     1981 aggtcgcggc gaccttcttc tcggtgacca gcctgcgata gccggcgacc gcggtctccg
     2041 gctgcccctg ggtgtcctcg gtgtacatga cgagcttccg gccaccgaga acgccaccca
     2101 tgaccgtgtt cacgtactcg acaccgagct cggcgccacg ctggatctgc tgtccgagcg
     2161 tcactgcgcc tggtgcggag agcggcgtct gcagcccgat cgcgacgtca gggccgcgcg
     2221 tcgcgctcgc ggccgtcgtg gccgcggcgc tcggtgcctg gctcgcggag agcggaccgg
     2281 tggccgctcc gcccccgcag gcacccacga tgaatgctcc gacgaccaag gtgatgatcc
     2341 acgaacgagc tctgatcact accccaacct ccccgcgtgc ggccgcggtc ccaattcgcg
     2401 ccgagtcttc aatagagtgc aagtgttgtc aatatattga attccagcga actgctcgct
     2461 cggccgggcg ccgctaccgg cggctgagtg ggcatccgcc gatcgccaca tcgcgtcggc
     2521 gagatggcgg ccgaacacag ttcagaagtc ctccatgcgg ataggtctgg ccggaacgcg
     2581 tggccggtgg cgcagcaggt cgggctgccc gcgaggcacc gcgatgcgcc ggagcacgtc
     2641 ctcgcacacg cgtccctggc agcgtcccat acatgcgcgg gtgcgcttct tcagcccggc
     2701 gatcgcgctc gccccctcct cgaacgcggc agcgacgcgc tcaagcggca cctcctcgca
     2761 gcggcagaca aggagcgtgg gttcggcggg cgcggggtat gagcgctcta cgcggcgggt
     2821 gggccgctcg tcccgccaga gcgcgtcaac gatggcgtgt ccctgctcga cctctgggag
     2881 gaagcggaac gcgagcgcgc
//
LOCUS       DASTZR010000204         2725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1134280, whole genome shotgun sequence.
ACCESSION   DASTZR010000204 DASTZR010000000
VERSION     DASTZR010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134280"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VFV20_04500"
     CDS             <1..249
                     /locus_tag="VFV20_04500"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU5287641.1"
                     /translation="VLIDSQGLLPSDAGAIVSLVDGNIKAVDGPFTEAKELVGGYAII
                     EVRSYEEALELGRRLMQIHKDHWPGFEGSVEIRQIAEF"
     gene            297..1580
                     /locus_tag="VFV20_04505"
     CDS             297..1580
                     /locus_tag="VFV20_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014690375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5287642.1"
                     /translation="MGDETTRAAIEAVWRLESTRLIAALVRIVRDVGLAEDFAQDAIV
                     AALAQWPSSGIPDNPGAWLMTTAKRRTVDAFRQRARNDETAAALARELSEGVEEDPTA
                     GIDHVEDDVLRLMFICCHPALTSEAQTTLTLKLVAGLSTREIARAYLAPEATIAQRVS
                     RAKRTLAEAGAAIEEPSGPERAERLETVLGVVYLLFNEGYSATEGDDWMRPALCDEAV
                     RLGRILTALVPDDPEVHGLAALMELQSSRLAARAGPDGEPILLDAQDRGQWDGARIRR
                     GLDALARADALLAAAGTGAEAGPYTLQAAIAAEHARAASVDDTDWDRVAELYERLGRR
                     TGSPVAELNRAVALGRARGPEAGLALLDQLDDVPALRGYHLVPSVRGDLYERQGRVAE
                     AAAEFERAAAMTANARERQLLQRRADAARAGTPPA"
     gene            complement(1606..2634)
                     /locus_tag="VFV20_04510"
     CDS             complement(1606..2634)
                     /locus_tag="VFV20_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010593498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEU5287643.1"
                     /translation="MDVPLYAWFAVLVAIVAMLAIDLFAHRRAHAIGVREALGWSVVW
                     VLAGITFGIVVWNAWGAEFGQQYFAGYLIEKSLAVDNVFVWAIIFASFAVPREYQHRV
                     LFLGVLGALVLRGLFIAAGAALIENFSWILYVFAAFLIVTGVRMLLTRNEHVHPERSR
                     TLRLFRRFVPMTDAYHGQRFLIRRAGAVVATPLLAVLVLVEVTDVIFAVDSIPAIFAV
                     TSEPFLVFTANAFAILGLRAMYFLLADLIHRFRYLKLGLALVLVWVGVKMALLDVLYI
                     PTPVSLAVVATIIAVSIGASLIATRGEGRHAVEAAPAGPFRIATASELASAEPVFRRS
                     RARARARS"
BASE COUNT          430 a         1000 c          981 g          314 t
ORIGIN      
        1 gtgctgatcg acagccaggg actgctgccg agcgacgccg gggcgatcgt ctcgctcgtc
       61 gacgggaaca tcaaggccgt cgacgggccg ttcaccgagg cgaaggagct cgtcggcggc
      121 tacgccatca tcgaggtccg ctcgtacgag gaggcgctcg agctcgggcg gcgactcatg
      181 cagatccaca aggaccactg gcccggcttc gagggcagtg tggagatccg gcagatcgcg
      241 gagttctgac ccgcccgacg cttgcacccg cgcggcgcgc gtggtctcat gctcgcgtgg
      301 gcgacgagac gacgcgcgcc gcgatcgagg ccgtctggcg gctcgagtcg acgcggctca
      361 tcgccgcgct cgtgcgcatc gtgcgcgacg tgggcctcgc cgaggacttc gcgcaggacg
      421 cgatcgtcgc ggcgctcgcg cagtggccct cctccggcat ccccgacaac ccgggagcct
      481 ggctcatgac cacggcgaag cgccgcaccg tcgatgcgtt ccgacagcgc gcccgcaacg
      541 acgagaccgc ggcggcgctc gcccgcgagc tctcggaggg ggtcgaggag gatccgaccg
      601 ccgggatcga ccacgtcgag gacgacgtgc tgcgcctcat gttcatctgc tgccatcccg
      661 cgctcacgtc ggaggcgcag accacgctca cgctgaagct cgtcgcggga ctctcgacgc
      721 gcgagatcgc ccgggcgtac ctcgcacccg aggcgaccat cgcccagcgc gtctcgcggg
      781 ccaagcgcac gctcgccgag gccggcgcgg cgatcgagga gccgagcggc cccgagcgcg
      841 ccgaacgtct ggagaccgtg ctcggcgtcg tctacctcct cttcaacgag ggctactcgg
      901 ccaccgaggg cgacgactgg atgcgccccg cgctctgcga cgaggccgtg cggctcggcc
      961 gcatcctcac ggcactcgtg cccgacgatc cggaggtcca cggcctggcc gccctcatgg
     1021 agctgcagtc gtcgcggctc gccgcgcgcg caggacccga cggcgagccg atcctgctcg
     1081 acgcccagga ccgcgggcag tgggatggcg cgcgcatccg ccgcgggctc gatgccctcg
     1141 cccgggccga cgcgctcctg gccgccgccg gcaccggcgc cgaggccggc ccgtacacgc
     1201 tgcaggcggc gatcgccgcg gagcacgcgc gcgcggcatc cgtcgacgac accgactggg
     1261 accgggtcgc cgagctctac gagcgcctcg ggcgccgcac gggctcgccc gtggccgagc
     1321 tcaaccgtgc ggtggcgctc ggacgggcgc gcggacccga agccgggctc gcgctgctcg
     1381 accagctcga cgacgtgccc gcgctgcgcg gctaccacct cgtgccgagc gtgcgcggcg
     1441 acctctacga acggcagggc cgcgtcgccg aggcagccgc cgagttcgag cgcgcggcgg
     1501 cgatgaccgc gaacgcgcgc gagcggcagc tgctccagcg ccgggcggac gccgcgcggg
     1561 cgggcacgcc gccggcctga ccgggccgtc gatgcgccgc gcgcgtcagc tccgtgcccg
     1621 tgcccgtgcc cgagatcgtc ggaacaccgg ctccgccgac gcgagctcgg atgccgtcgc
     1681 gatccggaac ggccccgcgg gtgccgcctc gaccgcatgc cgcccctctc cgcgcgtcgc
     1741 gatgagcgat gccccgatcg acaccgcgat gatggtcgcg acgaccgcga gcgacacggg
     1801 cgtcgggatg tacaggacgt cgagcagcgc catcttcacg ccgacccaca cgagcacgag
     1861 cgccagcccg agcttgaggt agcggaagcg gtggatgagg tcggcgagca ggaagtacat
     1921 cgcccggagc ccgaggatcg cgaacgcgtt cgccgtgaac acgaggaacg gctcgctcgt
     1981 gaccgcgaag atcgccggga tggagtcgac cgcgaagatc acgtcggtca cctcgacgag
     2041 caccagcacg gcgagcagcg gcgtcgcgac gaccgcgccc gcccgccgga tcaggaaccg
     2101 ctgcccgtgg taggcgtcgg tcatcggaac gaagcggcgg aacagccgga gcgtgcgcga
     2161 gcgctcggga tgcacgtgct cgttgcgcgt cagcagcatc cggacgcccg tgacgatgag
     2221 gaacgcggcg aacacgtaga ggatccacga gaagttctcg atgagcgcgg cgcccgcggc
     2281 gatgaacagc ccgcggagca cgagcgcgcc gagcacgccg aggaacagca cccggtgctg
     2341 gtactcgcgc ggcaccgcga acgaggcgaa gatgatcgcc cacacgaaca cgttgtcgac
     2401 cgcgagcgac ttctcgatca ggtagcccgc gaagtactgc tgcccgaact cggcgcccca
     2461 ggcgttccag acgacgatgc cgaacgtgat gccggcgagc acccagacga ccgaccagcc
     2521 gagcgcctcg cgcacgccga tggcatgcgc gcgacggtgg gcgaacagat cgatcgcgag
     2581 catcgcgacg atcgcgacga gcaccgcgaa ccaggcgtac aggggtacgt ccaacgtctc
     2641 ctccagttgt caggtcgtac ggcaaccgga ggtctcgtcc gagcgtcgcg ccggcggcgc
     2701 ggcccggatg cgccggggcc cgatg
//
LOCUS       DASTZR010000205         4337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_168084, whole genome shotgun sequence.
ACCESSION   DASTZR010000205 DASTZR010000000
VERSION     DASTZR010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168084"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="VFV20_04515"
     CDS             complement(<1..1077)
                     /locus_tag="VFV20_04515"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5287644.1"
                     /translation="MAIEDVLARIDPKEVTELALALGNIDSPTGSEGPAGQFVYDWLA
                     TNGFAPKKYALIPERFNVAAWIPGSGGGYSLVFNAHLDTTLRPDAVWSARDPGDPLYH
                     SAWVEGDSIYGDGVVNDKGPMAAFLVAAKAIREAGHPLKGDLIVSAVAGEISREPIEE
                     WQGPQYLSKDLGVRFMVTHGVVADYALVAEGTGFGIVGIEPGKAHFKVTVYSDGPRYY
                     TPYLPRPTGLADAPNAIVRAAAVIAKLEEWAYDYQTRNTYRGKMGTIVPKASVNAIRS
                     GYPFNVTSAPQVCSFYVDTRILPGANPMDVRDELRGVLRAAGVSGEVELYLYRPGFEA
                     KGAERLIETIERCHAQTFATPPAIV"
     gene            complement(1067..2317)
                     /locus_tag="VFV20_04520"
     CDS             complement(1067..2317)
                     /locus_tag="VFV20_04520"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5287645.1"
                     /translation="MSAEARATGIPRESFLVAVSVFLVIAATNIMTPLLPDIRNDFGV
                     SISTAGVIVGSYGLARLVTDLPAGFVIDAIGHRRVALAGLLLLVASSILGWNASTVEM
                     LVAARVGSGVAVALVTTVTLASLGASATAANRGKVMSLFHVANNIGIAAYPLIGGVVG
                     AAFGWRATFGVTVVLAILAGLILAPVLLRLRIKRESGAAGGKGDARVLHGRERTIALG
                     AINYGVIANMVNRHGFRNTVLPLYAATALGLGGVSIATAIALMALTGLLVATPGGMIG
                     DRFGRRRVIMTGLVAIAIGDLVFLFTHDLLTFLALSALIGFGDFFSSSQTALLSETVP
                     SQHRTKVLAGYRFSSDLGAFLGPVVLAAVMDLANAQSSIVLAAALLVSASLAAFLGVP
                     ARVDDRPGRATSALAGVGGASDGD"
     gene            complement(2314..3198)
                     /locus_tag="VFV20_04525"
     CDS             complement(2314..3198)
                     /locus_tag="VFV20_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5287646.1"
                     /translation="MPALVVPPGVEGVVSLVELPDPAWESRWTRIFAPAGLKERLLNY
                     TLFSLKHRGRVDAVRLPIHGLVVLAGPPGTGKTTLAGGLADRAAREIGESLLFVEIDA
                     HAFPSQLLGESQRGVARLFERTIPDIAARGKPTIVLLDEVESLAVSRSGASLETNPVD
                     VHRATDAVLEGVDEVARRCPNVTFVATTNHPAGLDVAFLSRADLVEELGLPGADAVRR
                     ILKDTIAEVVSAEKLDELALGALADACVAAKLDARRVRKLVLRAIASSRELALAPEKL
                     VIADVAAALRDEMDGARA"
     gene            complement(3201..4247)
                     /locus_tag="VFV20_04530"
     CDS             complement(3201..4247)
                     /locus_tag="VFV20_04530"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5287647.1"
                     /translation="MKTVTTTKVTHYEIQKKRRESLLDEWKGYHKTVLKRKDVVMHDV
                     PSRRMRRGVFAGWDGDRPTKNLDASIHEVPPHTTTTIHRHAWDAIQFVESGTGWTEID
                     GVRVDWKPWDTIHLPSWAWHRHGNDADRPAVFHTWSVQPMLEQFGVALLEEGGDTPVE
                     QLAPRPRQVQPLTGDDPYARRTQRLAKQAEGTESARLITRFDDVRGLVTKRGARSLFL
                     VDKSIGYHTAGLSAVMHELAPGLYQSRHRHGGEAWLYVVSGRGHSEMDGVSYPWEEGD
                     LIVVDHWAWHQHFNDSKDKTARLIRVHNFDALYDMIRILLDPLNLFEELPELDAPDLT
                     GVVWPDHFEGRPKA"
BASE COUNT          636 a         1533 c         1542 g          626 t
ORIGIN      
        1 cacgatcgcc gggggcgtcg cgaacgtctg cgcgtggcag cgctcgatcg tctcgatgag
       61 ccgctccgcg cccttcgcct cgaaccccgg gcggtacagg tacagctcga cctcgccgct
      121 cacgccggcg gcgcgcagca cgccgcggag ctcgtcgcgc acgtccatcg ggttcgcgcc
      181 cggcaggatg cgcgtgtcga cgtagaagga gcagacctgc ggcgcgctcg tcacgttgaa
      241 cggatagccg ctccggatcg cgttcacgct cgccttcggg acgatcgtgc ccatcttgcc
      301 gcgataggtg ttccgcgtct ggtagtcgta cgcccactcc tcgagcttcg cgatgaccgc
      361 ggccgcgcgc acgatcgcgt tcggcgcgtc cgcgaggccc gtcggccgcg ggaggtacgg
      421 cgtgtagtag cgcggaccat cgctgtacac ggtgaccttg aagtgcgcct tgcccggctc
      481 gatgccgacg attccgaagc cggtgccctc ggcgacgagc gcgtagtcgg cgacgacgcc
      541 gtgcgtgacc atgaagcgca caccgaggtc cttcgacagg tactgcggcc cctgccactc
      601 ctcgatcggc tcgcgcgaga tctctccggc gaccgcgctc acgatgaggt cgcccttcag
      661 cgggtggccg gcctcccgga tcgccttcgc cgcgacgagg aacgcggcca tcgggccctt
      721 gtcgttcacc accccgtcgc cgtagatgct gtcgccctcg acccacgcgg agtggtagag
      781 cgggtcaccg ggatcgcgcg ccgaccagac cgcgtccggc cgcagcgtcg tgtcgaggtg
      841 cgcgttgaag acgaggctgt agccaccgcc cgagcccggg atccacgccg cgacgttgaa
      901 gcgctccggg atgagcgcgt acttcttcgg cgcgaagccg ttcgtggcaa gccagtcgta
      961 cacgaactgg ccggcgggcc cctcgctccc ggtcgggctg tcgatgttgc cgagcgcgag
     1021 cgccagctcg gtgacctcct tcgggtcgat ccgtgccagc acgtcctcaa tcgccatccg
     1081 aagcccctcc cacaccggcg agcgcgctcg tggcgcgccc aggccggtcg tcgacgcgcg
     1141 cgggtacgcc gaggaacgcc gccagcgacg cgctcaccag cagcgccgcg gcgagcacga
     1201 tcgaggactg cgcgttcgcg aggtccatga ccgccgcgag gaccaccggt cccaggaacg
     1261 cgccgaggtc cgacgagaaa cggtagcccg cgagcacctt ggtgcggtgc tgcgagggga
     1321 cggtctcgga cagcagcgcc gtctgcgagc tcgagaagaa gtcgccgaag ccgatgagcg
     1381 cgctgagggc gaggaacgtg aggaggtcgt gggtgaacag gaacaccagg tcgccgatcg
     1441 cgatcgcgac gagcccggtc atgatcacgc gccggcggcc gaagcggtcg ccgatcatgc
     1501 cgcccggcgt cgcgacgagc agaccggtga gcgccatgag cgcgatcgcg gtggcgatgg
     1561 agacgccgcc caggcccagc gcggtcgccg cgtacaacgg caggaccgtg ttgcggaacc
     1621 cgtggcggtt gaccatgttc gcgatcacgc cgtagttgat cgctccgagc gcgatcgtgc
     1681 gctcacgccc gtggagcacg cgcgcgtcgc ccttgccgcc ggcagcgccg ctctcgcgct
     1741 tgatgcgcag gcgcagcagc accggcgcga ggatgagccc cgccaggatc gcgaggacca
     1801 cggtgacccc gaacgtcgcc cgccagccga aggccgcgcc gacgacgccg ccgatgagcg
     1861 gatacgcggc gatgccgatg ttgttggcga cgtggaacag gctcatgacc ttgccgcgat
     1921 tggccgccgt ggcgctggcg cccagcgacg cgagcgtcac cgtcgtgacg agcgcgaccg
     1981 ccacgccgga gccgacacgc gccgcgacga gcatctccac ggtgctcgcg ttccagccaa
     2041 ggatcgacga cgcgacgagc aggaggagcc cggcgagcgc gacccggcgg tgtccgatcg
     2101 cgtcgatcac gaaccccgcc ggcagatcgg tgaccaggcg cgcgaggccg tacgacccca
     2161 cgatgacgcc cgcggtggag atgctcacgc cgaagtcgtt ccggatgtcg gggaggagcg
     2221 gcgtcatgat gttcgtcgcc gcgatcacga ggaagaccga gaccgcgacg aggaacgact
     2281 cgcgggggat gccggtcgcg cgcgcctccg cgctcacgcg cgggcgccgt ccatctcgtc
     2341 gcgcagcgcc gcggccacgt cggcgatcac gagcttctcc ggcgcgagcg cgagctcgcg
     2401 gctggacgcg atggcgcgca gcacgagctt gcgcacgcgg cgggcgtcga gcttcgccgc
     2461 gacgcacgcg tcggcgagcg cgccgagcgc gagctcgtca agcttctcgg ccgagacgac
     2521 ctcggcgatg gtgtccttca ggatgcgccg caccgcgtcc gcgccgggca ggccgagctc
     2581 ctccaccagg tcggcacgcg agaggaacgc cacatcgagg ccggccgggt ggttcgtcgt
     2641 ggcgacgaag gtcacgttcg ggcagcgacg cgcgacctcg tcgacgccct cgagcaccgc
     2701 gtcggtcgcg cggtgcacgt cgacgggatt cgtctccagc gacgccccgc tccggctcac
     2761 cgcgagggac tccacctcgt cgagcagcac gatggtgggc ttgccgcgcg ccgcgatgtc
     2821 ggggatggtc cgctcgaaga gacgtgccac cccgcgctgg ctctcaccga ggagctggct
     2881 cgggaaagcg tgcgcgtcga tctcgacgaa gagcaggctc tcgccgatct cgcgggccgc
     2941 acggtcggcg aggccgcccg cgagggtcgt cttccccgtc ccaggcggcc cggccaggac
     3001 cacgaggccg tggatcggga gccggaccgc gtcgacgcgc ccgcggtgct tcagcgagaa
     3061 gagcgtgtag ttgagcagcc gctccttcag gcccgccggc gcgaagatcc gcgtccagcg
     3121 gctctcccac gccggatccg gcagctcgac gagcgagacc acgccctcga cgcccggcgg
     3181 caccacaagc gccggcatcg ctatgccttc ggtcgtccct cgaagtgatc gggccagacg
     3241 acgcccgtga ggtccggcgc gtcgagctcg gggagctcct cgaagaggtt cagcggatcg
     3301 aggaggatgc ggatcatgtc gtagagggcg tcgaagttgt ggacgcggat gagccgagcg
     3361 gtcttgtcct tcgaatcgtt gaaatgctgg tgccaggccc agtggtcgac gacgatgagg
     3421 tcgccctcct cccacgggta gctgacgccg tccatttcgc tgtgcccgcg gcccgagacg
     3481 acgtacagcc acgcctcgcc gccgtggcgg tgacgtgact ggtacaggcc gggcgcgagc
     3541 tcgtgcatca ccgccgagag gccggcggtg tgatagccga tcgacttgtc gacgaggaac
     3601 aggctgcgtg cgccgcgctt ggtgaccaga ccgcgcacgt cgtcgaagcg cgtgatgagc
     3661 cgagcgctct cggtgccctc cgcctgcttc gcgagacgct gcgtgcggcg cgcgtacggg
     3721 tcgtcaccgg tgagcggctg cacctgccgc ggccgcgggg ccagctgctc gaccggcgtg
     3781 tcgccgccct cttcgagcag cgccacgccg aactgctcga gcatcggctg cacgctccag
     3841 gtgtggaaca ccgccggcct gtcggcgtcg ttgccgtgcc ggtgccacgc ccagctcggc
     3901 aggtggatgg tgtcccacgg cttccagtcc acacggacgc cgtcgatctc ggtccaaccc
     3961 gtgcccgact cgacgaactg gatcgcgtcc caggcgtggc gatggatcgt cgtcgtcgtg
     4021 tgcggcggga cctcgtggat gctcgcgtcg aggttcttcg tggggcggtc gccgtcccag
     4081 ccggcgaaca cgccgcggcg catgcggcgg ctcggcacgt cgtgcatcac gacgtccttc
     4141 cgcttcagga cggtcttgtg atagcccttc cactcgtcca gcagcgactc gcgccgcttc
     4201 ttctggatct cgtagtgcgt gaccttcgtg gtggtgacgg tcttcatcgg gctccggccg
     4261 gcgccgcctg gacgcgcggc aggacctcct tggcgaggat ctcgatctgc ttcagatagt
     4321 ccgggaagcg catgcgg
//
LOCUS       DASTZR010000206         7021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_348944, whole genome shotgun sequence.
ACCESSION   DASTZR010000206 DASTZR010000000
VERSION     DASTZR010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7021
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348944"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="VFV20_04535"
     CDS             <1..1003
                     /locus_tag="VFV20_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287648.1"
                     /translation="VRLEPAAGKTIVHGVDLDGLPMLKVELSGDYRFSSAYGAAPSGF
                     SPNGRWLTLVSRDATESRFAVIDVEKNKLTQTVALGTRFTFDAIHNDATGMYLIEHPS
                     ATSTAYNVRLFDLRTRALSPGIIFDKTQIAQFDPTVGIMDGTFHVSVAPKTGDWSYGL
                     YMRPNGKPFVHALNVPGGYAQCIVDLAGTWTRDSRFAMALADDGRRLYVVDTAGGTVS
                     AIDALTQKVVRTSSFGARTGPGGPGATSAVVSPDGTRLYATAPRGIAMIQTSDLSVRG
                     WVAPDLAARALAISADGARLFALAADGVHAVELTSGRDLGVLAPAQGARALHLVTQR"
     gene            complement(1011..2084)
                     /locus_tag="VFV20_04540"
     CDS             complement(1011..2084)
                     /locus_tag="VFV20_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002623121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5287649.1"
                     /translation="MTVRELRVEGYRSIRSLRLALAPVTVILGPNGSGKTNLYRALRL
                     LTAAADGTLARSLADEGGMPSVLWAGPRKRSATARFTVTVDLEDMAYELACGLVPPPN
                     LPFELDPDVKEETVWLFDGKRKHVTAQRLGGTAFALGADGARATFPFELWNGESMLAQ
                     LSEPHRFPLLSALRGTLARWRFYHQFRTDPLAPARQPQVGVRTPALAHDGSDLAAALM
                     TVQEIGDRAGLHAAIEHAFPGARLELWSDRGRFGYRLAMPGLVRPLEAGELSDGTLRY
                     LYLVAALLSPRTPPFLALNEPETSLHPDLLPALAELIADAASRGQILVTTHAEPLARA
                     LEKRLRTPPIRLAKRDGATEVEE"
     gene            complement(2123..2728)
                     /locus_tag="VFV20_04545"
     CDS             complement(2123..2728)
                     /locus_tag="VFV20_04545"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU5287650.1"
                     /translation="MGTGADGHAVKATASPREEEILAAAAKAFREKGYHATSVRDIAE
                     SVGLLKGSLYHYIRSKEELLARLFEGALGDTIAELEAIAATDARPSERLRTMVRAYVD
                     AVTANLDAVAIYLREWRALPAPELARVRARRRAMRTLFEDVIAEGIRAKEFLPGDAKL
                     SALAILGMCNWIYEWYRPRGRLRPEQLADELAQRALRSVSR"
     gene            complement(2698..4410)
                     /locus_tag="VFV20_04550"
     CDS             complement(2698..4410)
                     /locus_tag="VFV20_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5287651.1"
                     /translation="MNYFLENPDLVTTFDRLVPWDRIVPLTQGKDANVAETVATWREM
                     LAVAGEYIGTEVAGRAKEVDEIGVVRDGGKVETSAPMQQNLKGLADLGFVGLSLPESL
                     GGSGLPFTVNSFGVEMIARACPATMVDFGIGFTEPAAMILRFGTDEQKQRYIPRLVRG
                     ESKGAVAMTEPQAGSDVGNVQTAARQDGGCWRLDGRKQFISAGNGDFVIVLARMVAGS
                     KGLDGLGLFLVDREGDNYLVERAEHKFTIRGSTTCSLVFEGSKAELLGEPGCGWREIT
                     TFMNESRIAIGIQGLGIAEAAYAAASRYASERVQMGRPIREHPMIAEMLLDMETTIAG
                     LRALATEASLLQDEVAFAKDEKAARKLRELTPLVKWYGSEEVIGVCRETMQIYGGYGV
                     VTEYEVERHMRNALILPIYEGTSQIQSLMAVRDLMRGVLRNPASLVRGGPTPTLAKAD
                     VGGDYAVARATLVGALRAMVATMARRGGLELVRGKRQPTEAEMAPFLLHAERITETLS
                     HLHVARVLGEQAKRLPERRPLAERAARRARLVALRNAAAMGAGDGGVFERIGEWERER
                     TATR"
     gene            complement(4407..6518)
                     /locus_tag="VFV20_04555"
     CDS             complement(4407..6518)
                     /locus_tag="VFV20_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEU5287652.1"
                     /translation="MTVDITTIAPAGARYEPAGADGVGRIVIDRANDSVNAIDPPLIA
                     ALLNAVDDARSARPRAIVVASAKPDNFSGGADLKLLSIWPSAAELSEGSRVMQRLCNE
                     LAGLPFTVVAALNGSALAGGFELALACDWRVAADRPDLRVGLPEIQLGLIPAAGGSQR
                     LPRLVGLPRALDMILSSRRVNARRALRSGLVDELVHPSVLDRAARERALKFGKRRPGG
                     GSTAVERAATNLAPARAFAFRTARERVMKETRGHYPAPLLTLDAMATGLAQGLAAGLE
                     AEARAFGELATSAVARSLIALFFLGMRQRQAASAGLPATAAPTDIAVVGAGLMGSGIA
                     QSAAVSGMNVRLRDVDEAALTKGLGAVRQLTEDAGRKGVVDRREAKRVIARVSGTTDW
                     SGFARAGLVIEAVFEELALKQRVLAEIEAVVRPDAVIASNTSALPIAEIATGAKHPER
                     VVGMHFFSPVHRMSLVEVVRHDSADPAAVARVVAAGQALGKTVIVVRDGPGFYTTRVI
                     GTMLGEATRLLKEGARIEDVDRAMTAFGWPEGPFLLMDALGLAVARHAGDTMAAALGV
                     SDEANVLQILVDAGLQGKRGGAGFYVYDGKKHTPNERVYAMIGAHPTQWQEDIAERLT
                     AVFVNAAARCIDDGVLRSPAEGDLGAVLGLGFPPFLGGPFRYADTRGTALRERLADLA
                     SRHGDLYVPAKSLTDGRRFYP"
     gene            complement(6515..>7021)
                     /locus_tag="VFV20_04560"
     CDS             complement(6515..>7021)
                     /locus_tag="VFV20_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEU5287653.1"
                     /translation="VFDRAHGTITAGNSSPLTDGAATVLLLSEERARAEGRTGLVAIR
                     SYAYAAVDPADQLLIAPAYAIPIALRRAGLALSDIGLIEMHEAFAAQVLSTFRVMEQN
                     GVGTVDQDRVNVMGGSIALGHPFGATGARIVTTLANEMRRRGTQFGLASVCAAGGNGA
                     AIVLELLA"
BASE COUNT          962 a         2547 c         2516 g          996 t
ORIGIN      
        1 cgtgcggctc gagcccgccg ctggaaagac gatcgtgcac ggcgtcgatc tcgatggact
       61 gcctatgctc aaggtcgagc tgtcgggcga ctaccgcttc tccagcgcgt acggcgcggc
      121 gcccagcggc ttctcgccga acggcaggtg gctcacgctc gtgtcacgcg atgcgaccga
      181 gagccgcttc gccgtcatcg acgtcgagaa gaacaagctg acgcagacgg tggcgctcgg
      241 cacgcgcttc acgttcgacg cgatccacaa cgacgcgacc ggcatgtacc tcatcgagca
      301 tccgagcgcg acctccaccg cgtacaacgt gcgcctcttc gacctgcgca cccgcgcgct
      361 cagccccggg atcatcttcg acaagacgca gatcgcgcag ttcgatccga ccgtgggcat
      421 catggacggg acgttccatg tatcggtcgc gccgaagacc ggcgactgga gctacggcct
      481 gtacatgcgg ccgaacggaa agccgttcgt gcacgcgctc aacgtccccg gcggttacgc
      541 gcagtgcatc gtcgatctcg ccgggacctg gacgcgcgac tcgcgcttcg cgatggcgct
      601 cgccgacgac ggccgccgtc tctacgtcgt cgacacggcc ggcggcaccg tctccgccat
      661 cgacgcgctg acccagaaag tggtgcgtac gtcatcgttc ggcgcgcgga cgggccccgg
      721 tggtcccggc gcgacgagcg cggtcgtctc acccgatgga acgcgcctct acgcgacggc
      781 cccgcggggc atcgcgatga tccagacgtc ggacctcagc gtgcgcggtt gggtcgcgcc
      841 ggacctcgcc gcgcgcgcgc tcgcgatctc ggcagacggc gcccgtctct tcgcgctcgc
      901 ggccgacggg gtccacgcgg tcgagctcac aagcggccgc gacctgggcg tcctcgcgcc
      961 tgcccagggc gcgcgggcgc tccatctcgt cacccagcgc tgagcgcggc tcactcctcg
     1021 acctcggtcg cgccgtcgcg cttcgcgagg cggatgggcg gcgtgcgcag gcgcttctcg
     1081 agcgctcgcg ccagcggctc ggcgtgcgtc gtgacgagga tctggccgcg cgatgcggcg
     1141 tcggcgatga gctccgcgag cgcgggcagg agatcggggt ggaggctcgt ctcgggctcg
     1201 ttcagcgcga ggaacggcgg cgtgcggggc gagaggagcg ccgccacgag gtagaggtag
     1261 cgcagcgttc cgtcggagag ctcgcccgcc tcgagcggac gcacgagccc gggcatcgcg
     1321 agccggtagc cgaagcgtcc gcgatcgctc caaagctcga ggcgcgctcc agggaacgcg
     1381 tgctcgatcg cggcatggag cccggctcgg tcgccgatct cctgcaccgt catgagcgcg
     1441 gccgcgagat cgctgccgtc gtgggcgagc gcgggcgtgc gcacgcccac ctgcggctgg
     1501 cgggcgggcg ccagcggatc cgtgcggaac tggtgataga agcgccagcg cgcgagcgtg
     1561 ccgcgaagcg cgctgagcag cgggaagcga tgaggctccg agagctgcgc gagcatcgac
     1621 tcgccgttcc agagctcgaa cgggaacgtc gcgcgggcgc cgtcggcccc gagcgcgaac
     1681 gctgtcccgc ccagccgctg cgccgtgacg tgcttccgct ttccgtcgaa cagccagacc
     1741 gtctcctcct tgacatccgg gtcgagctcg aatggcaggt tgggcggtgg cacgagtccg
     1801 cacgcgagct cgtacgccat gtcctcgagg tcgacggtga cggtgaagcg cgcggtcgcg
     1861 ctccgctttc gcgggccggc ccacaacacc gacggcatgc cgccctcgtc cgcgaggctg
     1921 cgcgcgagcg tcccgtcggc cgcggcggtg agcagccgga gcgcgcggta caggttcgtc
     1981 ttccccgagc cgttcggtcc gaggatcacc gtgaccggtg cgagcgcgag ccgcagcgag
     2041 cggatggagc ggtagccctc gacgcgaagc tcgcgcacgg tcatcgcggt gactctacgc
     2101 gaggcgtgac cgcgggcccg ggttagcggc tcacgctccg cagcgcgcgc tgcgcgagct
     2161 cgtcggcgag ctgctctggg cggagccgtc cgcgcgggcg ataccactcg tagatccagt
     2221 tgcacatgcc gaggatcgcg agcgccgaga gtttcgcgtc gccggggagg aactccttcg
     2281 cgcggatgcc ttcggcgatg acgtcctcga acagggtgcg catcgcgcgg cgccgcgcgc
     2341 gaacgcgcgc gagctcgggc gccggcaggg cgcgccattc gcggagatag atggcgacgg
     2401 cgtcgaggtt cgccgtgacc gcgtccacgt aggcgcggac catcgtgcgg agccgctcgg
     2461 acggacgcgc gtcggtcgcg gcgatcgcct cgagctccgc gatggtgtcc ccgagcgctc
     2521 cctcgaagag ccgcgcgagc agctcctcct tggagcggat gtagtgatag aggctgccct
     2581 tgagcagccc gacggactcg gcgatgtcgc gcacgctcgt ggcgtggtag cccttctcgc
     2641 gaaaggcctt cgccgcggcc gcgaggatct cctcctcgcg cgggctcgct gtcgccttca
     2701 ccgcgtggcc gtccgctccc gttcccattc gcctatccgc tcgaacacgc cgccgtcgcc
     2761 ggcgcccatc gccgcggcgt tccggagcgc gacgagccgc gcgcgccgtg ccgcgcgctc
     2821 cgcgagcgga cggcgttccg gcaggcgctt cgcctgttcg ccgaggaccc gggcgacgtg
     2881 gaggtgggag agcgtttcgg tgatgcgctc cgcgtgcagg aggaacggcg ccatctcggc
     2941 ctcggtcggc tgccgcttcc cgcgcaccag ctcgagcccg ccgcggcgcg ccatcgtcgc
     3001 gaccatcgcg cggagcgcgc cgacgagcgt cgcgcgcgcc accgcgtagt cgccgccgac
     3061 gtcggccttc gcgagcgtcg gcgtcggacc gccgcggacg agcgaggctg ggttacgcag
     3121 gacgccccgc atgagatcgc gcaccgccat cagcgactgg atctgcgacg tgccctcgta
     3181 gatcggcagg atgagcgcgt tgcgcatgtg ccgctcgacc tcgtactcgg tcacgacgcc
     3241 gtagccgccg tagatctgca tcgtctcgcg gcagacgccg atgacctcct ccgacccgta
     3301 ccacttcacc aggggcgtca gctcgcgcag cttgcgcgcc gccttctcgt ccttggcgaa
     3361 cgcgacctcg tcctgcagca gcgacgcttc ggtcgcgagc gcgcggagcc cggcgatcgt
     3421 cgtctccatg tccagcagca tctcggcgat catcgggtgc tcgcggatgg gccggcccat
     3481 ctggacccgc tcggacgcgt agcgcgacgc ggccgcgtac gcggcctcgg cgatgccgag
     3541 accctggatg ccgatcgcga tgcgcgactc gttcatgaac gtcgtgatct cgcgccagcc
     3601 gcagcccggc tccccgagca gctccgcctt cgagccctcg aagacgagcg agcaggtcgt
     3661 cgatccgcgg atggtgaact tgtgctccgc gcgctcgacg aggtagttgt cgccctcgcg
     3721 gtcgaccagg aagaggccga gtccgtcgag ccccttggag ccggcgacca tgcgcgcgag
     3781 cacgatgacg aagtccccgt tgcccgcgct gatgaactgc ttgcggccgt ccaggcgcca
     3841 gcaaccgccg tcctggcgcg ccgcggtctg cacgttgccc acatccgacc cggcctgcgg
     3901 ctcggtcatg gcgaccgcgc ccttgctctc cccgcgcacc aggcgcggga tgtagcgctg
     3961 cttctgctcg tcggtgccga agcgaaggat catcgcggcc ggctcggtga agccgatgcc
     4021 gaagtcgacc atcgtcgcgg gacaggcgcg cgcgatcatc tcgaccccga acgagttcac
     4081 cgtgaacggc agtccgctgc cgccgagcga ctcggggagt gagaggccca cgaagccgag
     4141 gtcggcgagg cccttcaggt tctgctgcat cggcgcgctc gtctcgacct tgcccccgtc
     4201 gcgcacgacg ccgatctcgt cgacctcctt ggcgcggccg gcgacctcgg tgccgatgta
     4261 ctcgccggcg acggcgagca tctcgcgcca cgtcgccacg gtctcggcga cgttcgcgtc
     4321 ctttccctgc gtcagcggga cgatgcggtc ccaggggacc agccggtcga acgtggtgac
     4381 gaggtcgggg ttctcgagga agtagttcat gggtagaagc gcctcccgtc ggtgagactc
     4441 ttcgcgggca cgtacagatc gccgtgacgc gatgcgagat ccgcgagccg ctcgcgaagc
     4501 gccgtgccgc gcgtgtcggc gtagcggaag ggaccgccga ggaacggcgg gaacccgaga
     4561 ccgaggacgg cgccgagatc gccctccgcc ggcgagcgca gcacgccatc gtcgatgcac
     4621 cgcgccgcag cgttcacgaa gaccgcggtg agccgctcgg cgatgtcctc ctgccactgc
     4681 gtcggatggg ccccgatcat cgcgtagacg cgctcgttcg gcgtgtgctt cttgccgtcg
     4741 tagacgtaga agcccgcgcc gccgcgcttt ccctgcagtc ccgcatcgac gaggatctgg
     4801 aggacgttcg cctcgtcgct gacacccagc gcggccgcca tcgtgtctcc ggcgtgccgc
     4861 gcgaccgcca gtccgagcgc gtccatgagc aggaaggggc cttcgggcca gccgaacgcg
     4921 gtcatcgcgc ggtcgacgtc ctcgatgcgc gcgccctcct tcagcagtcg ggtcgcctcg
     4981 ccgagcatcg tgccgatgac gcgcgtggtg tagaagccgg ggccgtccct cacgacgatc
     5041 acggtcttgc cgagcgcctg gccggcggcg acgacccgcg cgacggccgc cgggtcggcg
     5101 ctgtcgtggc gcaccacctc gacgaggctc atgcgatgca ccggcgagaa gaagtgcatg
     5161 cccacgacgc gctcgggatg cttcgcgccg gtcgcgatct cggcgatcgg gagcgccgac
     5221 gtgttcgacg cgatgaccgc gtcgggtcgc accacggcct cgatctcggc gagcacgcgc
     5281 tgcttcagcg cgagctcctc gaacaccgcc tcgatgacca ggccggcacg cgcgaacccg
     5341 ctccagtcgg tcgtcccgct cacgcgcgcg atgacgcgct tcgcctctct gcgatcgacg
     5401 acgcccttgc gcccggcgtc ctcggtcagc tggcgcacgg cgccgaggcc cttcgtgagc
     5461 gccgcctcgt cgacgtcgcg caggcgcacg ttcatccccg acaccgcggc cgactgcgcg
     5521 atgcccgatc ccatgaggcc cgcgcccacg accgcgatgt ccgtcggcgc ggccgtcgcg
     5581 ggaaggcccg ccgacgccgc ctgccgctgg cgcattccca ggaagaagag cgcgatgagg
     5641 ctgcgcgcga cggcgctcgt ggccagctcg ccgaaggcgc gcgcctccgc ctcgaggccg
     5701 gcggcgagtc cctgcgcgag accggtcgcc atcgcgtcca gcgtgaggag cggggcgggg
     5761 tagtggccgc gcgtctcttt catcacgcgc tcgcgcgccg tgcggaacgc gaacgcgcgc
     5821 gccggcgcga ggttcgtcgc ggcgcgctcc accgcggtcg acccaccgcc gggccggcgc
     5881 ttcccgaact tcagcgcgcg ctcgcgcgcg gcgcggtcga ggaccgacgg gtgcacgagc
     5941 tcgtcgacga ggccggaccg cagcgcacgg cgcgcgttca cgcggcgtga cgagaggatc
     6001 atgtcgagcg cgcggggcag tccgacgagg cgcggcagcc gctgcgaccc gccggccgcg
     6061 gggatgaggc cgagctggat ctccggtagg ccgacgcgca ggtccgggcg gtccgcggcg
     6121 acgcgccagt cgcaggcgag ggcgagctcg aacccgccgg cgagcgcgct gccgttcagc
     6181 gccgcgacga cggtgaaggg gaggcccgcg agctcgttgc agaggcgctg catgacacgg
     6241 ctcccctccg agagctccgc ggccgatggc cagatggaga gcaacttgag gtcggccccg
     6301 ccgctgaagt tgtcgggctt cgcgctcgcg acgacgatgg cgcgtggcct cgcgctacgc
     6361 gcgtcgtcga ccgcgttcag gagggcggcg atgagcggcg ggtcgatcgc gttgacgctg
     6421 tcgttcgcgc ggtcgatgac gatgcggccc acaccgtccg cgcccgccgg ctcgtaccgc
     6481 gcgcccgcgg gcgcgatcgt cgtgatatcg acggtcacgc gagcagctcg agcacgatcg
     6541 ccgcgccgtt gccgcccgcc gcgcacaccg acgccaggcc gaactgcgtc ccgcgccgtc
     6601 gcatctcatt ggccagcgtc gtgacgatgc gcgcgcccgt cgcgccgaag gggtggccga
     6661 gcgcgatgga accccccatg acgttcacgc ggtcctggtc caccgtccca acgccgttct
     6721 gctccatgac tcggaacgtg gagagcacct gcgccgcgaa cgcctcgtgc atctcgatga
     6781 gcccgatgtc ggatagcgcg agccctgccc gcctcagggc gatgggaatg gcataggcgg
     6841 gcgcgatgag gagctgatcg gccggatcga ccgccgcgta cgcgtaggag cggatcgcca
     6901 cgagcccggt gcgtccctcg gcgcgggcgc gctcctcgga cagcagcagc accgtggcgg
     6961 cgccgtcggt gagggggctc gagttgccgg cggtgatggt gccgtgcgcg cggtcgaaga
     7021 c
//
LOCUS       DASTZR010000207         3334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1056490, whole genome shotgun sequence.
ACCESSION   DASTZR010000207 DASTZR010000000
VERSION     DASTZR010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056490"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VFV20_04565"
     CDS             complement(<1..459)
                     /locus_tag="VFV20_04565"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEU5287654.1"
                     /translation="MGEGRLRTLAAICDTFVPGDGAALPPASALGVPELIRSELAALG
                     RPSLVAEMDLLLRLVENPLANLALVGRPVRFSRLGQERREAYMKTLGASPLPLKRLAF
                     QDLKRLALLYSFSSEGSPYPAAAGYQEPALDPPSPSALRARTPKPGETIDA"
     gene            complement(483..2003)
                     /locus_tag="VFV20_04570"
     CDS             complement(483..2003)
                     /locus_tag="VFV20_04570"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEU5287655.1"
                     /translation="MRVAHPLIASVVVFVASFCTLVLELIAGRILAPWLGVSLYTWTS
                     IIGVCLAGIAAGNYVGGKIADRFPTALTVGVQFVVSGVLALAILPATVWVIDAQLGRE
                     WPLMLRILVDTTIVFFLPTAVLGTISPVVIKLSLADLGRTGSIVGRIYAISTVGAIAG
                     TFATGFFLIDVMGTRAIVWWVAAALIAIGVVVARPWRKPALLTVAALAIGVLALAWYR
                     LPAFAAPCLRESNYYCIQIVDDYGMKALKLDQFVHSYVSTSDPTIIGYGYERTYQELT
                     DFHVRGRASFTTLAIGAGGYTFPRYLIAAYPHAKVDVLEIDPAVIEVARQYLGVPDDP
                     RLTSYSGDARLWFIEERPVGRYDIVYGDAFNDIAIPWHLTTLEFDRMIRRSLAPDGLL
                     MANIVDDFDRGDFLRASVNTLRQVFEYVYVFPREPQSATSHPTTFVLVASAAPLDLDE
                     FQKFDGPHTDLLKFSSAIAADDLLRYLEGGRRIVLTDDHAPADNLIAPVVNIRAAP"
     gene            complement(2077..2149)
                     /locus_tag="VFV20_04575"
     tRNA            complement(2077..2149)
                     /locus_tag="VFV20_04575"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2114..2116),aa:Thr,seq:tgt)
     gene            complement(2170..>3334)
                     /gene="rlmB"
                     /locus_tag="VFV20_04580"
     CDS             complement(2170..>3334)
                     /gene="rlmB"
                     /locus_tag="VFV20_04580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEU5287656.1"
                     /translation="AYTEQIRRVTAGPRSGAAPSHEAGEPIAGPHAVLEAIRAGRQVK
                     RLFISNERGTRTGPVNDLINEAQERRIFVRFVDPMEVQRLSPIENHQGVVAIVEGKAG
                     VELEELLLHLDTLNEPALVLAIDSLQDPQNFGVLLRSGEGAGIDGVIIPKHRAVGLTP
                     AVAKTSAGASEHLMIAEVANLRQAIDALKEKGIWVVGTDETGELLYDEVDYRGATGIV
                     IGGEGEGIRRLVLEGCDQVVRIPMEGSVASLNAAAAGTVILYEALRQRRRNVAPADTR
                     TPPPPRREALTPQEETMAMDGEDFAGDENEAEVAEGEAIPMDDGEALPAEAAVDADEG
                     TEDGADEQLDEVADEAPETEPEAVAEAPKKRAPRAKKATTARKTTTRKKTTDS"
BASE COUNT          535 a         1127 c         1152 g          520 t
ORIGIN      
        1 ggcgtcgatg gtctcgcccg gcttcggcgt gcgcgcgcgc agcgccgacg gggagggcgg
       61 gtcgagggcc ggctcctggt agccggccgc ggcgggatac ggtgatcctt cgctcgagaa
      121 cgagtacagc agcgcgagac gcttcaggtc ctggaacgcg aggcgcttca gcggcagcgg
      181 actcgcgccg agcgtcttca tgtacgcctc gcgccgctcc tggcccagcc gcgagaaacg
      241 cacgggccgt ccgacgagcg cgaggttcgc gagcgggttc tcgacgaggc gcagcagcag
      301 gtccatctcc gccacgaggc tcgggcgccc gagcgcggcg agctccgaac ggatcagctc
      361 gggtacgccg agcgcgctgg ccggtgggag cgccgcgccg tcaccgggaa cgaacgtgtc
      421 gcagatagcc gcgagcgtgc ggagccttcc ctcacccaca tgtcagagcg tacgccgcgg
      481 atctacggcg ctgcgcggat gttgacgaca ggcgcgatca ggttgtccgc gggcgcgtga
      541 tcgtccgtca gcacgatccg ccggccgcct tccaggtagc gcaagaggtc atccgcggcg
      601 atcgctgagg agaacttcag aaggtcggta tgcgggccgt cgaacttctg gaactcgtcg
      661 aggtccaggg gagcggccga ggcgaccagc acgaacgtcg tcggatggct cgtcgcggac
      721 tgcggctcgc gcgggaagac gtagacgtat tcgaacacct ggcggagcgt gttgaccgac
      781 gctcggagga agtcgccgcg gtcgaagtcg tcgacgatgt tcgccatcag cagtccgtcc
      841 ggcgcgagcg accggcggat catgcggtcg aattcgagag tcgtcagatg ccacgggatc
      901 gcgatgtcgt tgaacgcgtc gccgtacacg atgtcgtagc gcccgacggg acgttcctcg
      961 atgaaccaga ggcgcgcgtc gccgctgtag ctcgtgagcc gcggatcgtc cggaacgccg
     1021 aggtactgcc gcgcgacctc gatcaccgca gggtcgatct cgagcacatc caccttcgcg
     1081 tgcggatacg cggcgatgag gtacctcggg aacgtgtagc cgccggctcc gatcgcgagc
     1141 gtagtgaacg aggcgcgtcc acggacatgg aagtccgtaa gctcctggta cgtgcgctcg
     1201 tagccgtacc cgatgatggt cggatccgac gtcgagacgt agctgtggac gaactgatcg
     1261 agcttgagtg ccttcatccc gtagtcgtcc acgatctgga tgcagtagta gttcgactcc
     1321 ctgaggcacg gcgcggcgaa cgccggcaag cggtaccacg ccagggcgag gacgccgatc
     1381 gccagtgccg cgacggtgag cagggcgggc ttccgccacg gacgggctac gaccacgccg
     1441 atggcgatga gggccgcggc cacccaccag acgatggcac gggtgcccat gacatcgatg
     1501 aggaaaaagc ccgtcgcgaa cgtgccggcg atcgcgccga ccgtcgagat cgcgtagatg
     1561 cgtccgacga tcgatccggt gcggccgaga tctgcgaggg atagcttgat gaccactggc
     1621 gagatcgtcc ccaacaccgc ggtcgggagg aagaacacga tggtcgtgtc gacgaggatg
     1681 cgcagcatga gaggccactc ccgccccagc tgcgcgtcga tcacccatac ggtcgccggc
     1741 aggatcgcga gcgccagcac gcccgacacg acgaactgga cgcccacggt cagcgccgtc
     1801 gggaatcgat cggcgatctt gccgcccacg tagttccccg cggcgatgcc cgcgagacag
     1861 acgccgatga tgctcgtcca cgtgtacaga gaaacgccga gccagggagc gaggatccgc
     1921 ccggcgatca actcgagcac caacgtgcag aaactcgcca cgaagacgac cactgatgcg
     1981 atgagcgggt gcgccacgcg catgtcgctg gagcatacgt gccgcctgcc ggactgaaat
     2041 ggccgcggcg agcaccgacc ggccgcgcta cgtcggagcc gtccccgggt ctcgaacccg
     2101 gaaccggtgg tttacaaaac cactgctctg ccaattgagc taggacggcg cgaggtcaat
     2161 tgtcgcgcgc tacgagtcgg tcgtcttctt tcgcgtcgtg gtcttccggg cggtcgtcgc
     2221 cttcttcgcg cgcggcgccc gcttcttcgg cgcctcggcg acggcttcgg gctcggtctc
     2281 cggcgcttcg tcggcgacct cgtcgagctg ctcgtccgcg ccgtcctcgg tgccctcgtc
     2341 cgcatcgacg gccgcctcgg ccggcaacgc ttcaccgtcg tccatcggga tcgcctcgcc
     2401 ctcggccacc tcggcttcgt tctcgtcgcc ggcgaagtcc tcgccgtcca tcgccatcgt
     2461 ctcttcctgc ggtgtgagcg cctcgcgtcg cggcggcggc ggcgtgcgcg tgtccgcggg
     2521 cgcgacgttg cggcgccgct ggcgcagcgc ctcgtacagg atgaccgtcc cagccgcggc
     2581 cgcgttcaag gacgcgacag atccctccat cgggatgcgc acgacctggt cgcagccttc
     2641 caggacgaga cggcgtatgc cctcgccctc gccgccgatg acgatgccgg tcgcgccgcg
     2701 gtagtccacc tcgtcgtaga gcagttcacc ggtctcgtcg gtcccgacga cccagatgcc
     2761 cttctccttg agcgcatcga tcgcctgccg gaggttcgcg acctcggcga tcatcaggtg
     2821 ctcgctcgcg ccggcgctcg tcttcgcgac ggcgggcgtg agcccgaccg cgcggtgctt
     2881 cgggatgatg acgccgtcga tgcccgcccc ttcaccggag cgaaggagca cgccgaagtt
     2941 ctggggatcc tggagcgaat cgatcgcgag cacgagcgcg ggctcgttca gggtatccag
     3001 gtggagcagc agctcctcga gttcgacacc ggccttgccc tccacgatcg cgacgacgcc
     3061 ctggtgattc tcgatcggtg agaggcgttg cacctccatc ggatcaacga aacggacgaa
     3121 gatgcggcgc tcctgcgctt cgttgatgag atcgttcacc ggaccggtgc gcgtgccgcg
     3181 ctcgttcgag atgaacaggc gcttcacctg acgaccggcg cggatcgcct cgagcaccgc
     3241 gtgcggaccg gcgatcggct cgccggcctc gtgcgacggg gccgcgccgg agcgcgggcc
     3301 ggccgtcacg cgacggatct gctcggtgta cgcg
//
LOCUS       DASTZR010000208         5124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_247193, whole genome shotgun sequence.
ACCESSION   DASTZR010000208 DASTZR010000000
VERSION     DASTZR010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247193"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VFV20_04585"
     CDS             complement(<1..336)
                     /locus_tag="VFV20_04585"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU5287657.1"
                     /translation="MSLLERYTQPTEAQIPTVSHHGEIPSGQPTCTITIDGHEVTAVP
                     GEAILRAAQRAGFNIPTLCDDEKLAPAAACRMCLVNVEGYERPLPSCHLAVEPGMKVT
                     ATADGLFKLR"
     gene            complement(428..1642)
                     /locus_tag="VFV20_04590"
     CDS             complement(428..1642)
                     /locus_tag="VFV20_04590"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEU5287658.1"
                     /translation="MPSTSTASLLEARPAAAPHHDGSPDDLISAVERAGLRGRGGAGF
                     PLADKMRAVRAAALRTGREPLVVANAYDADPGSPLSRTLIARNVEGLVSGVAIAAHGV
                     GAREAIVYMHPEAQAERGTVERHIASRADRDGLAGLLVTVALGPGGFMGGEESALMNV
                     LESKRSMARQRPPYPADLGVQQRPTLISSAETLAWLPQVVHGAARADTKLVSVTGAVR
                     DPGVYEIPLGTTLAAILEKAGGVSGRLKGIHVGGPTGGILSAARADTRFDFDALRAAG
                     THMGSAQVRALTTDTCVVAEAAKLWSYLARETCGICVPCRVGTARVTGILESVQSQLG
                     RETDTKWLGELGDHMEKFSLCGFGITAPAILRTTMREFADDYRAHIAERRCPTNTCTP
                     VRSRRYETMAQP"
     gene            complement(1629..2351)
                     /locus_tag="VFV20_04595"
     CDS             complement(1629..2351)
                     /locus_tag="VFV20_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287659.1"
                     /translation="MFGILSGLVRTLEHLVRPKPVTRLYPYEKRELPERSRGLIALLL
                     EPETSIFKCESCLMCEKACPPRAISISYKFRNGFRKRPLLAPRASYYRIRMVQTAPYG
                     DRRPMSTAVVTVPAEEERDLDLAPVDRILREESSGLDRMTRVLYRTNEAYGYLPWTAA
                     SRIAAEFGKTMTDVYTAASLLANFRGAPDGKGTAIARGGRRYTGNVGVAGAWPPASPA
                     PAHDTLHEDEVHHVMEPAYAVD"
     gene            complement(2438..3514)
                     /locus_tag="VFV20_04600"
     CDS             complement(2438..3514)
                     /locus_tag="VFV20_04600"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEU5287660.1"
                     /translation="MGPQHPSTHGVLRLVLKLSGEKVVECIPVMGYLHRGLEKIFEWR
                     TYHQGIRYADQSDYVSNMLNEHAYAGAMEAIAGIDVPRRAEYIRVIVDEMSRCASHLV
                     WFGAYALDLGATTPILWCFRDREEILDMFEELCGSRMNFNYYRPGGVLYDLPQGWLAK
                     LERFLDRFEQDIERDFVRILEGNEIFLERTVGIGTIPAEVAKAYGLAGPMLRGSGINW
                     DLRLDRPYSIYPELKSVKAAVFPEGDAFARYKVRVQEMRNSIAILRECIANLPGGAMR
                     ARLGHVWKCPKGEAYYTVESSKGEVGIYMISDGRSPNPFRAKMRGASFHNLQILPWLL
                     NGRLVADVVAILGSIDIVLGEVDR"
     gene            complement(3621..4112)
                     /locus_tag="VFV20_04605"
     CDS             complement(3621..4112)
                     /locus_tag="VFV20_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEU5287661.1"
                     /translation="MGGEDMPARQAFLANDDNVVFTTVANLVNWARSRSPWPLGYGLA
                     CCAIEMMATGASTHDMSRFGMEVFRSSPRQADVMIVAGTVTHKMAPRLRRLYEQMPEP
                     KWVIAMGNCASSGGEFWDSYATLQGVDTVVPVDVYVPGCPPRPEALLEGLLRLREKIT
                     RGG"
     gene            complement(4237..>5124)
                     /locus_tag="VFV20_04610"
     CDS             complement(4237..>5124)
                     /locus_tag="VFV20_04610"
                     /inference="COORDINATES: protein motif:HMM:NF027305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin domain-containing protein"
                     /protein_id="HEU5287662.1"
                     /translation="QAEAQQEVTRRQTELAGLEAARKEQELVATTVKPAAAEAQAAIQ
                     RAEGEKRARIAAAEAGAEAERLQGSAEAAVVLTKGEAEAKALAMRADAYKLFNDAAII
                     QTVLAALPEIVRAAAEPMQHIDSLTVMSADGASEIVKNTSRVVAESATAIKGLTGMDV
                     PALIGAAMRSGNVGRVTASDGEGGAHPAASTPTVVPPTPATPAPSAQHAPADAAASTT
                     DGAEALEDEAARHFAQQLRGMPNVGQFAGLKLSEVSTIGPRPLRLLWQVAEPQIARRY
                     GSLTVGELMQRFGGQTAKH"
BASE COUNT          706 a         1815 c         1773 g          830 t
ORIGIN      
        1 gcgcagcttg aacagaccgt cggccgtcgc ggtcaccttc atgcccggct cgaccgcgag
       61 gtgacacgac gggagcggcc gctcgtagcc ctcgacgttc accagacaca tgcggcaggc
      121 cgcggccggt gcgagcttct cgtcgtcgca gagcgtcggg atgttgaagc cggcgcgctg
      181 cgcagcgcgc aggatggcct cgcccgggac ggcggtgacc tcgtggccgt cgatggtgat
      241 cgtgcaggtc ggctggcccg atgggatctc gccgtgatgg ctcacggtcg ggatctgcgc
      301 ctcggtcggc tgcgtatagc gttcgagaag gctcacgcct tctctccctt acccaacgcc
      361 gcgttgtcac tcggctgcag gactcgctcg ccgcggcgga gccgcggctc gctcgacttc
      421 atttcaatca cggctgcgcc atcgtctcat agcgtcgcga gcgcacgggc gtgcaggtgt
      481 tcgtcgggca gcgccgctcc gcgatgtgcg cgcggtaatc gtcggcgaac tcgcgcatcg
      541 tcgtgcgcag gatcgcgggc gcggtgatgc cgaacccgca gagcgagaac ttctccatgt
      601 gatcgccgag ctcaccgagc cacttcgtgt ccgtctcgcg gccgagctgg ctctgcacgc
      661 tctcgaggat ccccgtgacg cgcgcggtcc ccacacggca cggcacgcag atcccgcatg
      721 tctcgcgtgc gaggtacgac cacagcttcg ccgcctcagc gacgacgcac gtgtcggtcg
      781 tgagagcgcg gacctgggcc gagcccatgt gcgtgccggc cgcacggagc gcgtcgaagt
      841 cgaaccgggt gtccgcacgg gctgccgaca ggatgccgcc ggtcggcccg ccgacgtgga
      901 tgcccttcag ccgtccgctg acgccgccgg ccttctcgag gatcgcggcg agggtcgtgc
      961 cgagcggtat ctcgtacacg ccgggatcgc gcaccgcgcc cgtgaccgag acgagcttcg
     1021 tgtccgcgcg cgccgcgcca tggacgactt gtggcagcca cgccagggtc tccgcgctgg
     1081 agatgagcgt cggtcgctgc tgcacgccga ggtcggccgg gtacggcggc cgctggcgcg
     1141 ccatggaccg cttcgactcg aggacgttca tcagcgcgct ctcctcgccg cccatgaacc
     1201 cgcccgggcc aagggcgacg gtgacgagca gcccggcgag gccgtcgcgg tcggcgcgcg
     1261 acgcgatgtg ccgctcgacc gtgccccgct ccgcctgcgc ttcgggatgc atgtacacga
     1321 tcgcctcgcg cgcgccgacg ccatgcgccg cgatcgcgac gccggacacc aggccctcga
     1381 cgttgcgcgc gatgagcgtt cgcgagaggg ggctgcccgg atccgcgtcg tatgcgttcg
     1441 cgacgacgag cggctcgcgc cccgtccgga gcgctgccgc ccgcaccgcg cgcatcttgt
     1501 cggcgagcgg gaaacccgca ccaccgcggc cgcgcaggcc ggcacgttcg acggccgaga
     1561 tgaggtcgtc gggcgacccg tcgtgatgcg gcgccgcggc cggacgggcc tccagaaggc
     1621 tcgccgtgct agtcgacggc atacgccggc tccatcacgt ggtggacctc gtcctcgtgc
     1681 agggtgtcgt gcgcgggcgc cggtgacgcc ggcggccacg cgcccgccac gcccacgttg
     1741 ccggtgtagc ggcgtccgcc gcgcgcgatc gccgtgccct tgccgtcggg cgcgccgcgg
     1801 aagttggcga gcagggacgc ggcggtgtac acgtcggtca tcgtcttgcc gaactcggcg
     1861 gcgatccgcg acgccgcggt ccacggcagg tatccgtacg cctcattcgt ccggtacagg
     1921 acgcgcgtca tgcggtcgag accgctggac tcctcacgca ggatgcgatc gaccggggcg
     1981 aggtcgaggt cgcgctcctc ctcggcgggc accgtgacga cggcggtcga catgggacgg
     2041 cggtcgccgt acggcgcggt ctgcaccatg cggatgcggt aatacgacgc gcgcggcgcg
     2101 aggagcgggc gcttgcggaa gccgttgcgg aacttgtacg agatggagat cgcgcgtggc
     2161 gggcacgcct tctcgcacat gaggcacgac tcgcacttga agatgctcgt ctccggctcg
     2221 agcaggagcg cgatgaggcc gcgcgaccgc tccggcagct cccgcttctc gtacgggtag
     2281 aggcgcgtga cgggcttcgg acgcacgaga tgctcgagcg tgcggacgag gccgctcagg
     2341 atcccgaaca cttagcggtc cttctcgcgc ctacggcgcg agacccgctc atggctggtg
     2401 gtactcgctc ctcgcgggtg aaccgctcgc cgctcgctca tcgatcgacc tcccccagca
     2461 cgatgtcgat cgagccgagg atggccacga cgtcggccac gagccgcccg ttcaggagcc
     2521 acggaaggat ctgcaggttg tggaaggacg cgccgcgcat cttcgcgcgg aacgggttgg
     2581 ggctgcggcc gtcgctgatc atgtagatgc cgacctcgcc cttgctgctc tccaccgtgt
     2641 agtacgcctc acccttcggg cacttccaca cgtgtccgag ccgcgcgcgc atggcaccgc
     2701 ccggcaggtt cgcgatgcac tcgcgcagga tggcgatgga gttgcgcatc tcctgcacgc
     2761 gcaccttgta gcgcgcgaag gcgtcgccct ccgggaacac cgcggccttc acgctcttca
     2821 gctcggggta gatcgagtac ggacggtcga ggcgcagatc ccagttgatg cccgatccgc
     2881 gcagcatcgg gccggcgagg ccgtacgcct tcgccacctc ggccgggatc gtcccgatgc
     2941 cgacggtgcg ctcgaggaag atctcgttgc cctcgaggat gcgcacgaag tcccgctcga
     3001 tgtcctgctc gaagcggtcg aggaaacgct cgagcttcgc cagccacccc tgcggcaggt
     3061 cgtagagcac gccgcccggg cggtagtagt tgaagttcat gcgtgagcca cagagctcct
     3121 cgaacatgtc gaggatctcc tcacggtcgc ggaagcacca caggatcggt gtcgtggcgc
     3181 ccaggtccag cgcgtacgcg ccgaaccaca ccaggtgcga cgcgcagcgg ctcatctcgt
     3241 cgacgatcac gcggatgtac tcggctcggc gcggcacgtc gatgccggcg atcgcctcca
     3301 tcgcgcccgc gtaggcgtgc tcgttgagca tgttggagac gtaatccgac tggtccgcgt
     3361 accggatgcc ctggtgatac gtccgccatt cgaagatctt ctcgaggccg cggtgcaggt
     3421 atcccatcac cgggatgcac tcgacgacct tctcgccgga cagcttgagc acgaggcgga
     3481 ggacgccgtg cgttgacggg tgctgcggtc ccatgttgag gatgagctct tcggtctgga
     3541 gcccctcgtc ctcgatgacg ggaagctcga tgcgcgcacc ctcctggatc tggcggagct
     3601 cgctgtcggt ggtggtcagg tcagccgccc ctcgtgatct tttcccgcag acgcagcaga
     3661 ccctcgagga gcgcctcggg acgcggcggg cagccgggca cgtacacgtc gaccggcacc
     3721 accgtgtcga cgccctgcag cgtggcgtag ctgtcccaga actcgccgcc actcgaggcg
     3781 cagttcccca tcgcgatcac ccacttgggt tccggcatct gctcgtacag gcgccgcaga
     3841 cggggcgcca tcttgtgggt cacggtaccc gcgacgatca tcacgtccgc ctgacgcggg
     3901 ctcgagcgga acacctccat cccgaaccgc gacatgtcgt gcgtcgaggc gcccgtcgcc
     3961 atcatctcga tcgcgcagca cgcgaggcca tagccgagcg gccaggggct ccgcgaccgc
     4021 gcccagttca caaggttcgc gacggtcgtg aagacgacgt tgtcgtcgtt ggccaggaac
     4081 gcctggcgag ccggcatgtc ctcgccaccc atgcctgtct gcgtggtggt gggccggcgc
     4141 tggatgacgt cctcttcgaa gtcgatcggg acgcctttgg actgcgcggg gcgctcgtcg
     4201 atcgccatcg aggtgaagtc taaggaactt ccggccctaa tgcttcgcgg tttggcctcc
     4261 gaaccgttgc atcagttcgc cgacggtcag cgagccgtaa cggcgcgcga tctgcggctc
     4321 ggcgacctgc cagagcagcc gcaggggacg cggcccgatc gtgctgacct ccgacagctt
     4381 gaggccggcg aattgaccga cgttcggcat gccgcgcagc tgttgcgcga aatgcctcgc
     4441 cgcctcgtcc tcgagcgcct ccgcaccgtc cgtcgtgctc gccgcggcgt cagcgggcgc
     4501 gtgctgcgcc gacggcgcgg gtgtcgcggg tgtgggtggg acgaccgtag gcgtggaagc
     4561 ggccggatgg gcgcctccct ctccgtcgct cgcggtcacg cgaccgacgt tcccactgcg
     4621 catcgccgcg ccgatgagcg ccgggacgtc catgccggtg agccccttga tggccgtcgc
     4681 cgattcggcg acgacgcgcg aggtgttctt cacgatctcc gaggcgccgt ccgcgctcat
     4741 gacggtcagg gaatcgatgt gctgcatggg ttcggcggcc gcgcgcacga tctccggcag
     4801 ggccgccagc accgtctgga tgatcgcggc gtcgttgaac agcttgtacg cgtccgcgcg
     4861 catcgccagc gccttcgcct ccgcctctcc cttggtgagg accacggccg cctcggccga
     4921 gccctggagc cgctcggcct ccgcgcccgc ctcggcggcg gcgatgcgtg cccgcttctc
     4981 accctcggcg cgctggatcg cggcctgcgc ctccgcggcc gcgggcttca ccgtcgtcgc
     5041 gacgagctcc tgttccttgc gtgccgcttc gagccccgcg agctcggtct gccggcgcgt
     5101 gacctcctgc tgtgcctccg cctg
//
LOCUS       DASTZR010000209         2180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_586779, whole genome shotgun sequence.
ACCESSION   DASTZR010000209 DASTZR010000000
VERSION     DASTZR010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586779"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VFV20_04615"
     CDS             <1..553
                     /locus_tag="VFV20_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5287663.1"
                     /translation="MQVVATANDAPSAIEAARTHRPDVLVLDISMPGGGGLEVARRVR
                     EMELPTKIVVLTMYAEDRYVMEAVRLGAAGYVLKRSADKELIDAIRSVAAGEAYLTPA
                     AVRLLLATQQNEAGRPEPALSPREREVLRFTARGFSNLEIAERLFVSPKTVDTYRSRI
                     MAKLDLHRRSELVDYALRHGLLE"
     gene            550..735
                     /locus_tag="VFV20_04620"
     CDS             550..735
                     /locus_tag="VFV20_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287664.1"
                     /translation="MTKLTHAVTDDGTVFCPRRKQVVDVMACYGCEKLVEIDLDSRRP
                     KVVCAVPAAGELSAAGR"
     gene            complement(716..868)
                     /locus_tag="VFV20_04625"
     CDS             complement(716..868)
                     /locus_tag="VFV20_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287665.1"
                     /translation="MQIDLATILLACAGLGVAVAFAILAGTVSARVFFDATRGEEESA
                     PSAPRR"
     gene            1081..1686
                     /locus_tag="VFV20_04630"
     CDS             1081..1686
                     /locus_tag="VFV20_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HEU5287666.1"
                     /translation="MAATKLGKGTVIQDPPIARFLFSDTRMSLVWLLVRVYVGWAWLD
                     AGWHKVIDTGAKTNYVIDGAGIQAFWNRIAAIPAPPAKPTITYDWYRSYIQFMIDNHW
                     EGFFGKVIAYGEVAVGLGLIFGAFTGVAAVAGAFMNLNFMLAGSSSTNPVLLLLGFLL
                     VLAWKTAGYIGLDRYLLPILGTPWRVPTTERQTVVPVPTSV"
     gene            1780..>2180
                     /locus_tag="VFV20_04635"
     CDS             1780..>2180
                     /locus_tag="VFV20_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287667.1"
                     /translation="MRPIAARQSTYPRRLRIAAMVLPSLLIAGLVGLDYFLVERLFPV
                     GVSHLVTIVIGVAGVLAFSTAVFNQLSALQARDRANTARLRALADALEEKRTQLQAVN
                     AAGLALTSELDRDAVLQRVADQARLVTNAKYA"
BASE COUNT          348 a          760 c          710 g          362 t
ORIGIN      
        1 catgcaggtc gtcgccaccg ccaacgacgc gccgtccgcc atcgaggcgg cccgcaccca
       61 tcgacccgac gtgctcgtcc tggacatctc catgccgggc ggtggcgggc tcgaggtcgc
      121 ccgacgcgtg cgcgagatgg agctgccgac gaagatcgtc gtgctcacga tgtatgcgga
      181 agaccgctac gtcatggagg ccgtgcgcct cggtgcggcg ggatacgtgc tcaagcgttc
      241 ggcggacaag gagctcatcg acgcgatccg ttccgtggcc gccggcgagg cgtacctcac
      301 gccggccgcg gtgcggctcc tgctcgccac gcagcagaac gaggccggcc gccccgaacc
      361 cgctctgtcg ccgcgcgagc gcgaggtgct ccggttcacc gcacgcggat tctcgaacct
      421 ggagatcgcc gagcggctct tcgtgagccc gaagaccgtc gacacgtacc gctcgcggat
      481 catggcgaag ctcgatctgc accgacggtc ggagctcgtc gactacgccc tccgccacgg
      541 cctgctcgag tgacgaagct gacgcatgcc gtcacggacg acggcaccgt gttctgcccg
      601 cgacggaaac aggtcgtcga cgtcatggcg tgctacggat gcgagaagct cgtcgagatc
      661 gatctggatt cgcggcgtcc gaaggtcgtg tgcgcggtcc cggccgcggg agagctcagc
      721 gccgcggggc gctaggggcg ctctcctcct cgccgcgcgt cgcgtcgaag aagacccgcg
      781 cactcaccgt cccggcgagg atcgcgaatg cgaccgcgac cccgagcccg gcacacgcca
      841 ggaggatggt ggcgagatcg atctgcatgc ggcggatgct ccggcctgag ccgggacaca
      901 ggtagggcca aaggtccctg agggcgccgt cgccggtcat gtcagccctg cgacggtgcc
      961 ggtggccact cttggcaaga ccttcctgag agggtccgac ggaccaggcc tttggtccta
     1021 gcggccctgt tgaggggcgg tgcctgaccg caaagtgcct gtcaaaggag gtcacacgaa
     1081 atggccgcga cgaagttggg caagggtacc gtgatccagg acccgcccat cgcacgattc
     1141 ctcttctccg acacgcgcat gtctctcgtc tggcttctcg tccgcgtcta cgtcggatgg
     1201 gcatggctgg acgcgggctg gcacaaggtc atcgacacgg gcgccaagac gaactacgtc
     1261 atcgatggcg ccggcatcca ggcgttctgg aaccggatcg ccgccatccc cgctccgccg
     1321 gccaagccga cgatcaccta cgactggtat cgcagctaca tccagttcat gatcgacaac
     1381 cactgggagg gtttcttcgg caaggtgatc gcgtatggcg aagtggccgt cggcctcgga
     1441 ctgatcttcg gcgccttcac cggcgtcgcg gcggtcgcgg gcgccttcat gaacctgaac
     1501 ttcatgctcg cgggcagctc cagcacgaac cccgtcctgc tcctgctcgg cttcctcctc
     1561 gtgctcgcat ggaagacggc agggtacatt ggactcgaca ggtacctgct gcccattctc
     1621 gggacacctt ggagggttcc gacgaccgaa cgtcaaaccg tcgtcccggt ccccacgtcg
     1681 gtctagggcc ttcagacctc tggcacagga ggagcggagc aatccgctcc tcttgtgttt
     1741 cctgcgcatc ccatttcgcc cgttcctgat aatcgacaca tgagaccgat cgccgcgcgt
     1801 cagagcacgt atccacggcg actccgcatc gcggcgatgg tgctgccgtc gctgctcatc
     1861 gcgggcctcg tcggcctcga ttacttcctc gtcgagcggc tcttcccggt cggcgtctcg
     1921 catctcgtga cgatcgtcat cggcgtcgcg ggcgtgctgg cgttctcgac cgcggtgttc
     1981 aaccagctct ccgcgctcca ggcgcgagac cgcgcgaaca ccgcccgtct gcgagccctg
     2041 gccgacgccc tcgaggaaaa gcgcacgcag ctgcaggccg tgaacgcggc cggcctcgcg
     2101 ctcacgtcgg agctcgaccg ggacgcggtg ctgcagcgcg tcgccgacca ggcgcgcctc
     2161 gtgacgaacg cgaagtacgc
//
LOCUS       DASTZR010000210         2039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1239279, whole genome shotgun sequence.
ACCESSION   DASTZR010000210 DASTZR010000000
VERSION     DASTZR010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239279"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VFV20_04640"
     CDS             <1..316
                     /locus_tag="VFV20_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEU5287668.1"
                     /translation="AFEQVTAIMAEISTASSEQARGISQVNQTVTQLDSVTQQNAQLV
                     QRSVSVAGGLRREAERLGAAVKAFIIDKENRNEPQLPPPPVALAGGARRPGSRVRVRA
                     DA"
     gene            228..1022
                     /locus_tag="VFV20_04645"
     CDS             228..1022
                     /locus_tag="VFV20_04645"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU5287669.1"
                     /translation="MSPNYRRPLWRSLAALAALAAACACAQTLEVVGSSSAKKRLFGP
                     TEAALKAATGIEVKQVGASSGTGLLTVVEGRAAVTATSATLPETIDAASKDAQEKRKS
                     FTPPANLVFHEIAQDRVVAIVHKDNPVPSLTKAQLKDIFSGKVTNWKAVGGADAPIKV
                     ITGTSASGTRVHVTKEVMGGAEWVASAKEMRTSADELKAVSLDRNGIGAIGEEVVTAS
                     AGAVKTVPGYALVRPVGFITLGAPNPTVQKMIDFLKSPEARKLYAR"
     gene            1097..2011
                     /gene="lpxC"
                     /locus_tag="VFV20_04650"
     CDS             1097..2011
                     /gene="lpxC"
                     /locus_tag="VFV20_04650"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEU5287670.1"
                     /translation="MIRQRTLKTSVRATGVGLHTGRKVTMVLRPAPADTGIVFCRSDL
                     PGNPAIPAVAGNVTNTQMATVLEKDGARVSTVEHLMAALFGLGIDNAFVDVSAEEVPI
                     MDGSAGTFVFLLQTAGIEEQAAIKRYLRILKTVELEKDGKKVRLEPFDGFKVGFAIEF
                     RHPVFQQSATTVEVDFGEVSFVREISRARTFGFTQEVEYLRSRGLARGGSLDNAVVID
                     DFRVLNADGLRMDDEFVKHKALDAVGDLYLAGHPIIGAFYGHKSGHELNNLLVRKLLA
                     DRSAWEEATFDDVAQLPRAFANLQLQPA"
BASE COUNT          356 a          692 c          689 g          302 t
ORIGIN      
        1 cgccttcgag caggtcaccg cgatcatggc ggagatctcg accgcgtcga gcgagcaggc
       61 gcgcggcatc tcgcaggtca accagacggt gacgcagctc gacagcgtca cgcagcagaa
      121 cgcgcagctc gtgcagcgat cggtgtcggt cgcgggcggg ctgcgccgcg aggccgagcg
      181 cctgggcgcc gccgtgaagg cattcatcat cgacaaggag aaccgcaatg agccccaatt
      241 accgccgccc cctgtggcgc tcgctggcgg cgctcgccgc cctggcagcc gcgtgcgcgt
      301 gcgcgcagac gcttgaagtc gtcgggtcga gcagcgcgaa gaagcgcctc ttcggcccga
      361 ccgaggccgc gctgaaggcc gcgaccggca tcgaggtgaa gcaggtcggc gcgagctcgg
      421 gtacgggcct cctcacggtc gtcgagggac gcgccgcggt caccgccacg tcggccacgc
      481 ttcccgagac catcgacgcg gcaagcaagg acgcgcagga gaagcgcaag agcttcacgc
      541 cgcccgcgaa cctcgtgttc cacgagatcg cccaggaccg cgtggtggcg atcgtgcaca
      601 aggacaaccc cgtgccgtcg ctcacgaagg cgcagctgaa ggacatcttc tcgggcaagg
      661 tcacgaactg gaaggcggtg ggcggcgccg atgcgccgat caaggtcatc accgggacgt
      721 cggcgagcgg tacgcgcgtg cacgtcacca aggaggtgat gggcggggcc gaatgggtcg
      781 cctccgcgaa ggagatgcgc acgtcggccg acgagctgaa ggccgtgagc ctggaccgca
      841 acggcatcgg cgccatcggc gaagaggtcg tcacggcttc cgccggcgcg gtgaagaccg
      901 ttcccggcta cgcgctggtc cgcccggtgg gcttcatcac gctgggagcc ccgaacccga
      961 ccgtgcagaa gatgatcgat ttcctcaagt cgccggaggc gcgcaagctg tacgcccgct
     1021 aggcattcgc gccggaaaag ctaacgcaat gatagaattg cgtcttttcc ggcattcggc
     1081 ccgcggccga agcgcgatga tccgacagag aaccctgaag acgagcgtcc gtgccacggg
     1141 cgtcggcctt cataccggcc ggaaggtcac gatggtcctg cgtcccgctc ccgcggacac
     1201 gggcatcgtc ttctgccgct cggatctgcc cggcaacccc gcgattcccg ccgtcgcagg
     1261 aaacgtcacc aatacccaga tggccaccgt cctcgagaag gacggcgcgc gcgtctccac
     1321 cgtcgagcac ctcatggccg cgctgttcgg cctcggcatc gacaacgcct tcgtcgacgt
     1381 gtcggccgag gaagtgccga tcatggacgg gagcgccggg accttcgtct tcctcctcca
     1441 gaccgcgggg atcgaggagc aggccgccat caagcgctac ctgcgcatcc tgaagacggt
     1501 ggagctcgag aaggacggca agaaggtccg cctcgagccc ttcgacggct tcaaggtggg
     1561 attcgcgatc gagttccgcc acccggtgtt ccagcagtcg gccacgaccg tggaggtcga
     1621 tttcggcgag gtctcgttcg tgcgcgagat ctctcgcgcg cgcaccttcg gcttcaccca
     1681 ggaagtcgaa tacctgcgct ccagggggct tgcccgcggc ggctccctcg acaacgccgt
     1741 ggtgatcgac gatttccgcg tgctgaatgc cgacggcctg cgcatggacg acgagttcgt
     1801 gaagcacaag gcgctggatg cggtgggcga cctgtacctc gcgggccatc cgatcatcgg
     1861 cgcgttctac gggcacaagt cggggcacga gctgaacaac ctcctggtgc gcaagctcct
     1921 cgccgaccgc tcggcgtggg aagaggctac cttcgacgac gtggcccagc tgccgcgcgc
     1981 cttcgcgaac ctgcagctgc aaccggcctg agtgctggtc gtccgcatca tcgtcctgt
//
LOCUS       DASTZR010000211         7155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1108963, whole genome shotgun sequence.
ACCESSION   DASTZR010000211 DASTZR010000000
VERSION     DASTZR010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7155
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108963"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VFV20_04655"
     CDS             <1..489
                     /locus_tag="VFV20_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287671.1"
                     /translation="ELDLVVAAFHSKLRVREDQTARYLRAIANRHVNVLAHPRGRRYD
                     ARLGLIADWDRVARAAAERDLALEIDSWPDRQDLDVRSLAAVAAAGTRVAIDTDAHRP
                     QELAFSAFGIATAIRAGIAMDRVVNFLPADELLAWARASTQMARRSWAGAGTSRPDAA
                     AG"
     gene            complement(426..1361)
                     /locus_tag="VFV20_04660"
     CDS             complement(426..1361)
                     /locus_tag="VFV20_04660"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU5287672.1"
                     /translation="MGSGVRYQEVTVKSALTRVQGMPFAWSLNPYRGCAHACRYCYAR
                     EYHARMGRDTGAGFDREIEVKINFADVLRLELRRLRLRETVALGTGTDPYQPCEGRYR
                     LTRRALEALVASPLPLVVVTKGTLVVRDIDLLMKLARTVPVRVCVSLATVDPAIARHA
                     EPEAPAPRARLEALRRLRAAGIHAGILAAPVLPDLTDSDASLDAVAAAARAHDASFFS
                     IRPLKLDPGVRPHYFAFLSEQFPVLLPSAAQRFADRVNQDRRYVDDLERRVERIRAAH
                     GFGDERFGVQTAKDAQPAAASGRDVPAPAQLRLAI"
     gene            complement(1385..1891)
                     /locus_tag="VFV20_04665"
     CDS             complement(1385..1891)
                     /locus_tag="VFV20_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287673.1"
                     /translation="MAAEMDVTSLPHATLARTDHRCVIGMLVHRVAAATDPDRPSLVA
                     DALDRMLRSGHFEGCCVPRYLALAPERAPREVQIPIDKSGDVAARVIVWPIGSRDTDH
                     PHTDGWTVFVPVRGELATIERTDDGSRVAALAPRQPIVLRPEEGIRHLVRNAGSSPAL
                     SIHVSGRI"
     gene            2059..2184
                     /locus_tag="VFV20_04670"
     CDS             2059..2184
                     /locus_tag="VFV20_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287674.1"
                     /translation="MRRDASGSFTADQVKTGRSVARDRKTDAKHTAPKGMKDRGD"
     gene            2273..2812
                     /locus_tag="VFV20_04675"
                     /pseudo
     CDS             2273..2812
                     /locus_tag="VFV20_04675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
     gene            2821..3729
                     /gene="ligD"
                     /locus_tag="VFV20_04680"
     CDS             2821..3729
                     /gene="ligD"
                     /locus_tag="VFV20_04680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEU5287675.1"
                     /translation="MLATLSKDVVSGPEWLFEEKYDGIRAIAERTRSGVRLVSRTGQD
                     LTAGFADVAAAVARLDDDDLVLDGELVVFDRAGVSRFQLLQRRGVDPRTRPTYVVFDV
                     LRSRGRDLVRRPLEERRARLLELVPRKSGPLMPSRLLRGDGAAALATARDKGWEGVIA
                     KVRGSAYEPGQRSSSWRKVKVRGEAEFVIGGYTPPQGSRAEFGALLVGMFDGPKLRFV
                     GKVGTGYTAETLRDLGAKLRKLRTAGPPFDPAPRITGATWVRPALVAQLAYQEWTADG
                     KLRQPAFLGLRTDKRPKEITWRAREQ"
     gene            3708..4604
                     /gene="ligD"
                     /locus_tag="VFV20_04685"
     CDS             3708..4604
                     /gene="ligD"
                     /locus_tag="VFV20_04685"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEU5287676.1"
                     /translation="MARARAVKGGAVTIGGVAVTSAAKLWFPDDGITKGDIARFYDAV
                     SARILPWLRDRPLTAERCPDGMAGQCFYQKDFPLDRPPADVPRVTLRASSTGKDVHYL
                     VGGTKRALIAMVNLGCIAMHVMNARTAHLREPDWLAFDLDPTSGEFADAARAGLALRR
                     LLDEARLPSYPKTSGSRGLHVFVPLRAGQPGDRVAAYAVEVGDELARREPRLVTMAHA
                     KSERRGRVYADPFRNAYAQTIVAPYSVRRRPGATVSTPLAWDEVTPRLDPSRFTVRTF
                     EKRAARGDPWMDFKRHAVRLPS"
     gene            4675..5190
                     /locus_tag="VFV20_04690"
     CDS             4675..5190
                     /locus_tag="VFV20_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017910123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HEU5287677.1"
                     /translation="MATSRERSEHRVSSDTKPPQSGIAHTFSRAASWASAAMGSPWAF
                     LTAVGVILVWALTGPIFGFSSDWQLVVNTGTTIVTFLMVFLIQNTQNRDARAMHLKLD
                     ELIRAVPKARNEFMEAEEEDLDEIEREKQIVDQADPAPPEHKREERQEARANGRRANG
                     KNGHARGTTRS"
     gene            complement(5187..5552)
                     /locus_tag="VFV20_04695"
     CDS             complement(5187..5552)
                     /locus_tag="VFV20_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287678.1"
                     /translation="MTLAEVRRAQIAYWVFGAVWPLVSMRTFEMVTGNKRDEWLVRTV
                     ALLMLSTVATLAAMRPGVSDDRAMRVLGATSTAALGSVALIGPLVRRISKVYYVDALV
                     DLGLAALWVIAPTRRERAA"
     gene            5688..5822
                     /locus_tag="VFV20_04700"
     CDS             5688..5822
                     /locus_tag="VFV20_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287679.1"
                     /translation="MNELDARPETADRLQESVRRALALVGPGDSGRERASDEDLPPAA
                     "
     gene            complement(5819..6202)
                     /locus_tag="VFV20_04705"
     CDS             complement(5819..6202)
                     /locus_tag="VFV20_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5287680.1"
                     /translation="MKRDGGGELRKVLVVDDDDDLADVVRQALRDAGYSVATVRHGAA
                     ALELVKHIEPELILLDLSMPIMDGWSFVAQYRRIGKHGARIVLLTGNASASEIARTLG
                     ADGYITKPFDMKDLLAVVGRELTLS"
     gene            6313..>7155
                     /gene="ribA"
                     /locus_tag="VFV20_04710"
     CDS             6313..>7155
                     /gene="ribA"
                     /locus_tag="VFV20_04710"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEU5287681.1"
                     /translation="MAIRHLGGHPDRAEARLPSRFGAFRIAVYRLTGVETEVVALTHG
                     DVRTHLPVLVRLHSECLTGESLGSLRCDCGEQLSAALEQIGRSERGVLLYLRHEGRGI
                     GLFDKIRAYALQDDGLDTVDANVALGLPVDARDYASAAAVLRRLGVRRARLLTNNPAK
                     IEALSRHGVDVVERVPLEAPANAVSLPYLRTKQRRMGHLLTALDAEALRTAARTGLPA
                     VTIHYAQTLDGRIATRTGDARWVSGDASLRLAHELRASHDAVLVGIGTVLKDDPRLTV
                     RLADG"
BASE COUNT         1114 a         2483 c         2594 g          964 t
ORIGIN      
        1 gagctcgacc tcgtcgtggc cgcgttccac tcgaagctcc gcgtgcgtga ggatcagacc
       61 gcgaggtacc tgcgcgcgat cgcgaaccgg cacgtgaacg tcctcgcgca tccgcggggc
      121 cgccgctacg acgcgagact cggcctgatc gctgactggg accgtgtcgc gcgtgccgcc
      181 gcggagcgcg atctcgcgct cgagatcgat tcgtggccgg accgccagga cctcgacgtg
      241 cgctcgctcg ccgccgtcgc ggccgccggc acgcgcgtcg cgatcgacac ggacgcccat
      301 cggccgcagg agctcgcgtt ctccgcgttc ggcatcgcga ccgccatccg cgcgggcatc
      361 gcgatggacc gggtcgtgaa cttcctcccc gcggatgagc tcctggcgtg ggcgcgcgcc
      421 tcgactcaga tggcgaggcg cagctgggcc ggtgccggca cgtcgcgacc ggacgccgcc
      481 gcgggctgag cgtccttcgc ggtctggacc ccgaagcgct cgtcgccgaa gccgtgcgcg
      541 gcgcggatcc gctcgacgcg ccgctccaga tcgtccacgt accgccggtc ctggttgacg
      601 cgatccgcga agcgctgcgc ggccgacggc aacagcaccg ggaactgctc ggagaggaac
      661 gcgaagtaat gcggccgcac gccggggtcg agcttcaggg gccgtatcga gaagaacgag
      721 gcgtcatgcg cgcgggccgc ggccgcgacc gcgtcgagcg aggcgtcgct gtcggtgaga
      781 tcgggcagca cgggggcggc gaggatcccc gcatggatgc ctgccgcacg cagccgtcgc
      841 agcgcctcga gacgcgcgcg tggtgccgga gcctctggct cggcgtgccg cgcgatggcg
      901 ggatcgaccg tcgcgagcga gacgcagacg cgcaccggca cggtccgcgc gagcttcatg
      961 aggagatcga tgtcgcggac gacgagggtg cccttcgtga cgacgacgag gggcagcggt
     1021 gaggcgacga gcgcctcgag cgcgcggcgc gtgagccggt agcggccctc gcacggttga
     1081 tagggatcgg tgccggtacc gagggccacg gtctcgcgca gccgcaggcg ccgcagctcc
     1141 agccggagca cgtccgcgaa gttgatcttc acctcgatct cgcgatcgaa gcccgcgccc
     1201 gtgtcgcggc ccatgcgcgc gtggtactca cgcgcgtagc aataacggca ggcgtgggcg
     1261 cagccgcggt acgggttcag gctccacgcg aagggcatgc cctgcacgcg cgtgagcgcg
     1321 cttttgaccg tgacctcctg gtaccggacg ccgctcccca tccggctatc atagaacaga
     1381 tgttctagat acggccggag acatggatcg agagcgccgg cgatgagccg gcattccgca
     1441 cgaggtggcg gatgccctcc tcgggccgca gcacgatggg ctgccgtggc gcgagcgccg
     1501 cgacgcgcga tccgtcgtcg gtgcgctcga tcgtcgcgag ctctccccgc acggggacga
     1561 agacggtcca gccatcggtg tggggatggt cggtgtcgcg ggagccgatg ggccacacga
     1621 tgacgcgagc cgccacatcg cccgacttat cgatcgggat ctggacctcg cgcggcgcgc
     1681 gctcgggcgc gagggcgaga tatcgcggga cgcagcagcc ctcgaagtga cccgaccgca
     1741 gcatcctgtc gagcgcatcg gccacgagcg aagggcggtc cgggtccgtc gccgcggcga
     1801 cacggtggac gagcatgccg atgacgcagc ggtgatcggt ccgcgccagc gtcgcgtggg
     1861 gcagtgaggt cacgtccatc tcggcggcca tctcgtcctc cttcgaacga ccagcaggaa
     1921 gcacggcagg tgccaaacgc ggagagctgg cgaccctcat gcagtaccgc ggagcacgat
     1981 ggaggtgtac ggatggcccg gaaaggcgcg cccacaggca agcgagagac gatcagtccg
     2041 aacggaccga gtcgatacgt gcgccgcgac gcaagcggca gcttcaccgc ggaccaggtg
     2101 aagaccgggc ggtcggtggc acgtgaccgg aagaccgacg cgaagcacac cgcgccgaag
     2161 gggatgaagg accgcggcga ctaagaaggc gacacgaccg aggcggcagg gcctcgacgt
     2221 ctatcgccgg aagcgcgact tcacgcggac ggccgagccg agaccgaagc gcacggccgg
     2281 cggcgggcct ctgcgcttcg tcgtacagaa gcatcgcgcg acagccctgc actacgactt
     2341 ccgcctcgag gcccgcggca cgatgcgctc ctgggccgtg ccgaaggggc cgtcgttcga
     2401 tccgaagcag cgccgtctcg cgatggaggt ggaggaccac cccctcgagt acgcgaattt
     2461 cgagggcatc atccccgagg gtgagtacgg cggcggcacc gtgatgatct gggacgaggg
     2521 agtctgggcg cccatcgacg acgtcgatcc cgcgaaggcc atcgactccg gtgagatccg
     2581 cttcgtcctc tccggaaaga agctgaaagg tggctggacg ctggtgcgca ccggcgaccg
     2641 caagtggctg ctcatgaagc gccgcgacga atacgtgcgc gacgtcgaca tcgaagagag
     2701 cgagccgcgc tcggtgcgca cgaagcgtct gctcgccgag atcgcaaaag acgagggcgg
     2761 ggacgtgaag aaggccgcga cgggagatcc caagcgacgc ggcgcagcgt gatccggccg
     2821 atgctcgcca cgctctcgaa ggacgtcgtc agcggtccgg agtggctctt cgaggagaag
     2881 tacgacggca tccgcgcgat cgccgagcgc acgcggtccg gggtgcggct cgtgtcgcgg
     2941 accggtcaag acctcaccgc cggattcgcc gacgtggccg cggccgtcgc gcgcctcgac
     3001 gacgacgacc tcgtgctcga cggcgagctc gtcgtcttcg accgcgcggg cgtctcgcga
     3061 ttccagctgc tgcagcgtcg cggggtcgat ccgcgcaccc gccccaccta cgtcgtcttc
     3121 gacgtgctgc gttcccgagg ccgcgacctc gtgcggcggc cgctcgagga gcggcgcgcg
     3181 aggctgctcg agctcgtgcc gcggaagtcc gggccgctca tgccgtcgcg cttgctgcgc
     3241 ggcgacggcg ccgccgcgct cgcgaccgcg cgcgacaagg gctgggaggg cgtcatcgcg
     3301 aaggtgcggg gatcggcgta cgagccggga cagcgatcgt cgtcgtggcg caaggtcaag
     3361 gtgcgcgggg aggcggaatt cgtgatcggc gggtacacgc cgccgcaggg ctcgcgcgcg
     3421 gagttcggcg cgctcctcgt cgggatgttc gacgggccga agctgcgctt cgtcggcaag
     3481 gtgggcacgg gctataccgc cgagacgctg cgcgaccttg gtgcgaagct gcggaagctg
     3541 cgcaccgccg ggccgccgtt cgaccctgcg ccgcggatca cgggcgcgac gtgggtccgt
     3601 cccgcgctcg tcgcgcagct cgcgtatcag gaatggaccg ccgacggcaa gctgcgacag
     3661 cccgcgttcc tcgggctgcg caccgacaag agaccgaagg agatcacgtg gcgcgcgcgc
     3721 gagcagtgaa gggtggcgcc gtgacgatcg gcggcgtcgc ggtgacgagt gccgccaagc
     3781 tctggttccc ggatgacggc atcaccaagg gcgacatcgc gcgcttctac gacgcggtct
     3841 ccgcgcgcat ccttccctgg ctgcgtgacc ggccgctcac cgcggaacgc tgtcccgacg
     3901 gcatggccgg gcagtgcttc tatcagaagg acttccccct ggaccgcccg cccgcggacg
     3961 tcccgcgcgt caccctgcgc gcgtcgagca ccggcaagga cgtgcattac ctcgtcggcg
     4021 ggacgaagcg cgcgctcatc gcgatggtga acctgggatg catcgcgatg cacgtgatga
     4081 acgcgcgcac cgcccatctt cgcgaaccgg actggctcgc gttcgacctc gatccgacga
     4141 gcggcgagtt cgccgatgcc gcgcgtgcgg gcctcgcgct gcgccgcctg ctcgacgagg
     4201 cgcggctccc ctcgtatccg aagacgtcgg gctcacgcgg gctgcacgtc ttcgtccctc
     4261 ttcgtgcggg acagccgggc gatcgcgtcg cggcgtacgc ggtcgaagtg ggggacgagc
     4321 tcgcgcgccg cgagccgcgg ctcgtcacga tggcgcacgc gaagtcggag cggcgcgggc
     4381 gcgtctatgc ggacccattc cggaacgcat acgcgcagac gatcgtcgcg ccgtattcgg
     4441 tgcggcgcag gcccggggcc acggtctcga ccccgctcgc gtgggacgag gtcacgccgc
     4501 ggctcgaccc gtcccgcttc accgtgcgga cgttcgagaa gcgggccgcc cgcggggatc
     4561 cgtggatgga cttcaagaga catgcggtga gactgccctc gtaacgggcc acggcaaggc
     4621 gatcgacgct tgccggcacg gggggctggc atatcgcttg cgagccgtgt ctgcatggcg
     4681 acgtcacggg agcgcagcga gcaccgggtc tcatccgaca cgaagccgcc gcagtcgggc
     4741 atcgcccata ccttcagcag ggcggcgtcc tgggcatcgg ccgcgatggg aagcccgtgg
     4801 gccttcctca ccgccgtcgg cgtgatcctg gtgtgggccc tcaccggtcc gatcttcggc
     4861 ttctcgtcgg attggcagct cgtcgtgaac accggaacga cgatcgtgac gttcctcatg
     4921 gtgttcctga tccagaacac gcagaaccgc gatgcgcgcg ccatgcatct caagctcgac
     4981 gagctgatcc gtgccgtgcc gaaggcgcgg aacgagttca tggaggccga ggaagaggac
     5041 ctcgacgaga tcgagcgcga gaagcagatc gtcgaccagg ccgatccggc gccgccggag
     5101 cacaagcgcg aggaacgcca ggaagcgcgc gcgaacggcc ggagggcgaa cgggaagaac
     5161 ggccacgcgc gcggcacgac gcgaagctag gcggcgcgct ctcgacgagt cggtgcgatg
     5221 acccagagcg ccgcgagccc gaggtccacg agcgcgtcga cgtagtagac cttcgagatg
     5281 cgcctcacga gcggcccgat gagcgcgaca ctgccgagcg ccgccgtgga cgtcgcgccg
     5341 agcacgcgca tcgcccgatc gtcggagacg ccgggacgca tcgccgcgag cgtggcgacc
     5401 gtgctcagca tgaggagcgc gacggtccgc acgagccact cgtcgcgctt gttgccggtc
     5461 accatctcga acgtgcgcat gctgaccagc ggccagacgg ccccgaacac ccagtacgcg
     5521 atctgggcac ggcgcacctc cgcgagcgtc atcacggcct gtcgagcacg atccgttccg
     5581 gcacacagcg gcgagccgtc gctccgccgg ccgagagggc gcgatgcggc ccgctcatac
     5641 ggcccgattc gtgcagccaa acaagtcacc actgaacgga gggaccgatg aacgagcttg
     5701 acgcgcgccc cgagacggcg gatcgactgc aggaatccgt gcgccgcgcg ctggctctgg
     5761 tcggccctgg cgactccggt cgcgagcggg catccgacga ggatctcccg ccggccgcct
     5821 aggagagcgt gagctcccgg cccacgaccg cgagcaggtc cttcatgtcg aacggctttg
     5881 tgatgtagcc gtcggcgccg agcgtccgcg cgatctccga ggccgaggcg ttccccgtca
     5941 agaggacgat gcgtgcgccg tgcttcccga tccgccggta ctgcgcgacg aacgaccagc
     6001 cgtccatgat cggcatcgac aggtcgagaa ggatcagctc cggctcgatg tgcttcacga
     6061 gctcgagggc cgccgcgccg tggcgcaccg tggcgacgga gtagccggcg tcgcgcagcg
     6121 cctggcggac cacgtctgcc aggtcgtcgt cgtcatcgac gaccaggacc ttccgaagct
     6181 caccaccacc atcgcgcttc atcaagggct cattccggcc gcttgcaaga tagatgccga
     6241 cggctcgctt gcccggtgcg cggagggcgt ccgcccgccg cgccgggcgc gcgccttgct
     6301 cggtgggtgc gtatggccat acggcatctg ggcggccacc ccgaccgggc ggaggcgcgg
     6361 cttccgtcgc gcttcggtgc cttccgcatc gcggtgtacc ggctcaccgg cgtcgagacc
     6421 gaggtggtcg ccctcacgca cggcgacgtg cgcacgcacc tgcccgtgct cgtgcgactt
     6481 cactccgagt gcctgaccgg cgagtcgctc ggctcgctgc gctgcgactg cggggagcag
     6541 ctctcagcgg cgctcgagca gatcggccgc agcgagcgcg gtgtgctcct gtacctgcgc
     6601 cacgagggcc gcggcatcgg gctcttcgac aagatccgcg cgtacgcgct ccaggacgac
     6661 gggctcgaca cggtcgacgc caacgtcgcg ctcggcctgc cggtcgacgc gcgtgactac
     6721 gcctccgccg cggccgtgct gcgacggctg ggcgtgcggc gcgcccggct gctcaccaac
     6781 aacccggcga agatcgaagc gctgtcgcgg cacggcgtgg acgtcgtcga gcgggtcccg
     6841 ctcgaggcac ccgcgaacgc ggtgagcctg ccgtacctgc gcacgaagca gcgtcggatg
     6901 gggcacctgc tcacggcgct cgacgccgag gctctgcgga cggccgcgcg cacggggctc
     6961 cccgcggtga cgatccacta cgcccagacg ctcgacgggc gcatcgcgac ccgcaccggt
     7021 gacgcgcggt gggtgagcgg cgacgcgagc ctgcggctcg cgcacgagct gcgcgcgtcg
     7081 cacgatgccg tgctcgtcgg catcgggacg gtcctgaagg acgatccgcg tctcacggtg
     7141 cggctcgccg acggc
//
LOCUS       DASTZR010000212         2778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_716846, whole genome shotgun sequence.
ACCESSION   DASTZR010000212 DASTZR010000000
VERSION     DASTZR010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716846"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1818
                     /gene="aceE"
                     /locus_tag="VFV20_04715"
     CDS             <1..1818
                     /gene="aceE"
                     /locus_tag="VFV20_04715"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEU5287682.1"
                     /translation="FRGSGWNVIKVIWGREWDDLLSRDVDGALVNRMNETTDGESQRM
                     VIADGAYIREHFFGGDARLQQLVAHLSDDDLRKLRRGGHDYRKVYAAYKAATEHVGAP
                     TVILAQTVKGWALGPSVEGRNITHQAKKLSYEELKVFRDRLDLPIPDDRLMEDPPYYH
                     PGADSPEMRYMVERRRALGGFVPRRIAFPANIGQPKDDAFAEFPKGSGKAAASTTTAF
                     TRLLRNLLRDPNIGKRVVPIIPDEARTFGMDPLFKEVGIYSALGQRYEPVDAETLLPY
                     REATDGQVLEEGITEAGSMASLTAAGTSYATHGEAMVPFYIFYSMFGFQRTMDQIWAF
                     GDERGRGFLMGGTAGRTTLTGEGLQHDDGHSQLLATAIPIIRAYDPAFAYETAVIVRD
                     GIRRMFANNEDVFYYVTLYNENYPMPAMPEGVEEGILRGIYLFRRGGEGRRRVTLLGS
                     GPILLQALRAQELLQERFDVAADVYSVTSYQLLRNEALETERWNRLHPSERPRIPYVA
                     QVLGGKTPIVAATDYMKTVADQIARWVPQPYIPLGTDGYGRSDTRAALRRHFEVDAES
                     IAVASLHGLQQCEQYSAADVTKAIAELGIDPAKPEPRTA"
     gene            1896..2309
                     /locus_tag="VFV20_04720"
     CDS             1896..2309
                     /locus_tag="VFV20_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5287683.1"
                     /translation="MPRTCGMAEERAEAQAVPVRSPGRTVLVVDDDEDIRSVVRDVLE
                     EEGYRVVAVEGPDDARPALTAERVSLVLLDATTPRAMSGTQEDGLADPHPPIVMFSAA
                     VDLEARAKRVGAVGWIAKPFEIDELLAVVARYTAA"
     gene            complement(2274..>2778)
                     /locus_tag="VFV20_04725"
     CDS             complement(2274..>2778)
                     /locus_tag="VFV20_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5287684.1"
                     /translation="VLRHELPDLVVLMLTSDESDETMLAAIEAGASGYLLKDEPAGNV
                     VEAVRRAASGATLIPASVVQRLLAVRREQDAARSRAERAIPALTPREKTVLALMERGL
                     TAKAVAEELGISWDTARDHIQNVIEKLDAHSKPEAVFRARQLGLLVAGSAEPPESGGR
                     VPRHDRQ"
BASE COUNT          450 a          972 c          958 g          398 t
ORIGIN      
        1 ttccgcggat cgggctggaa cgtcatcaag gtgatctggg gccgcgagtg ggacgatctg
       61 ctctcgcgcg acgtcgacgg cgcgctcgtg aatcgcatga acgagacgac cgacggcgag
      121 tcgcagcgca tggtcatcgc cgacggcgcg tacatccgcg agcacttctt cggcggcgac
      181 gcgcggctgc agcagctcgt cgcgcatctc tccgacgacg acctgcgcaa gctccgccgc
      241 ggcggccacg actaccgcaa ggtgtacgcg gcgtacaagg ccgcgaccga gcacgtcggt
      301 gcgccgacgg tgatcctggc gcagaccgtg aagggatggg cgctcggccc gtcggtcgag
      361 ggccgcaaca tcacgcacca ggcgaagaag ctctcgtacg aagagctcaa ggtcttccgc
      421 gaccgcctcg acctgccgat ccccgacgac cggctcatgg aggacccgcc gtactaccac
      481 ccgggcgcgg atagccccga gatgcgctac atggtcgagc gccgtcgcgc cctcggcggg
      541 ttcgtaccgc gacgcatcgc gtttcccgcg aacatcggcc agccgaagga cgacgcgttc
      601 gcggagttcc ccaagggatc cggcaaggcc gcggcgtcga cgacgaccgc attcacgcgg
      661 ctcctgcgca acctgctgcg tgaccccaac atcggcaagc gcgtcgtccc gatcatcccg
      721 gacgaagcgc gcacgttcgg catggatccg ctcttcaagg aggtcggcat ctattcggcg
      781 ctcggccagc gctacgagcc ggtcgacgcg gagacgctcc tcccctaccg cgaggcgacg
      841 gacgggcagg tgctcgagga gggcatcacc gaggcgggct ccatggcgag cctcaccgcc
      901 gcgggcacga gctacgcgac gcacggcgag gcgatggtgc ccttctacat cttctattcg
      961 atgttcgggt tccagcgcac gatggaccag atctgggcgt tcggcgacga gcgcggccgc
     1021 ggcttcctca tgggcgggac cgcggggcgg accacgctca ccggcgaggg gctccagcac
     1081 gacgacggcc acagccagct tctcgccacc gcgatcccga tcatccgcgc ctacgatccc
     1141 gcgttcgcgt acgagacggc ggtcatcgtc cgcgacggca tccgccgcat gttcgccaac
     1201 aacgaggacg tcttctacta cgtcaccctc tacaacgaga actaccccat gccggcgatg
     1261 cccgagggcg tcgaggaagg gatcctccgc ggcatctacc tgttccggag gggtggcgag
     1321 ggacgccgcc gcgtcacgct gctcggatcg gggccgatcc tgctccaggc gctgcgtgcg
     1381 caggagctgc tccaggagcg gttcgacgtg gccgccgacg tgtacagcgt cacgagctac
     1441 cagttgctgc gcaacgaagc gctcgagacc gagcgctgga accggctgca cccctcggag
     1501 cgtccgcgca tcccgtacgt ggcacaggtg ctcggcggca agacgccgat cgtcgcggcc
     1561 accgactaca tgaagacggt cgcggaccag atcgcgcgct gggtgccgca gccgtatatc
     1621 ccgctcggca ccgacggcta cggccgcagc gacacgcgcg cggcgctgcg ccggcacttc
     1681 gaggtcgacg ccgagagcat cgccgtcgct tcgctccacg gtctccagca gtgcgagcag
     1741 tacagcgccg ctgatgtcac gaaggcgatc gccgaactcg ggatcgaccc cgcgaagccg
     1801 gagccgcgca cggcgtagcg ctcgcgccag gatcggttca gcctgtccac aggctcatcc
     1861 acatatccac atggaccgca cacgatggtc gtcgtatgcc ccgcacatgc gggatggcgg
     1921 aggagcgggc ggaggcgcag gctgtccccg tgaggagtcc agggaggacc gtcctcgttg
     1981 tcgacgacga cgaggacatc cgttcggtcg tgcgcgatgt cctcgaagag gagggctacc
     2041 gcgtcgtcgc ggtcgaaggg cctgacgacg cgcggcccgc actcaccgcg gagcgcgtct
     2101 cgctggtcct gctcgacgcg acgacgccgc gggccatgag cggcacgcag gaggacgggc
     2161 tggcggatcc gcatccgccc atcgtcatgt tcagcgcggc cgtggacctg gaggctcggg
     2221 ccaagcgggt gggcgcggtg gggtggatcg cgaagccgtt cgagatcgac gagctactgg
     2281 cggtcgtggc gcggtacacg gccgcctgat tccggcggct ccgccgagcc agcgacgagc
     2341 agcccgagct ggcgggctcg gaacaccgct tcgggcttgc tgtgcgcgtc gagcttctcg
     2401 atcacgttct ggatgtggtc gcgcgcggtg tcccagctga tgcccagctc ctccgcgacg
     2461 gccttggccg tcaggccgcg ctccatcagg gcgagcaccg tcttctcacg aggcgtgagc
     2521 gcggggatcg cgcgctccgc acgggaccgc gcggcgtcct gctcacgtcg caccgccagc
     2581 aggcgctgga cgacggacgc gggtatcagc gtcgcacccg acgcggcgcg gcgcaccgct
     2641 tcgaccacgt tgccggcagg ttcgtccttc agcaggtacc cggatgcacc cgcctcgatc
     2701 gcggcgagca tcgtctcgtc ggactcgtcg ctcgtgagca tgagcacgac gagatcagga
     2761 agctcgtggc gaaggacg
//
LOCUS       DASTZR010000213         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_663967, whole genome shotgun sequence.
ACCESSION   DASTZR010000213 DASTZR010000000
VERSION     DASTZR010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663967"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VFV20_04730"
     CDS             complement(<1..736)
                     /locus_tag="VFV20_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287685.1"
                     /translation="MTKAAARSAFQYGLAIFTLTALLGLANATQLFGALDRNTLLTHL
                     HSGTLGWITMGVIGVTIWLFGATGMNTAVRVSAASTAAYVIAFWSGNFQARAVFGVTE
                     LAVILYWWWFAASRGMAEGGLARLDITKLSVIFGLTTLVIGSTLGVIVQVILGTGNAL
                     PQTTDFVGAHASAQTGGYLVLVAAGVIEWLLTGGGRRSVAGLIQVALLFVGGLILSFS
                     LLLTAQLGEQLAQALPGIATLLTLAGI"
     gene            complement(805..1446)
                     /locus_tag="VFV20_04735"
     CDS             complement(805..1446)
                     /locus_tag="VFV20_04735"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5287686.1"
                     /translation="MDMTWLGHACFRMRGREGIVLTDPPDPKSGHAIPRTEAGLVTIS
                     HDHAGHSSLRSVAGEPVVLLGPGEYEVHDVLVTGIGSFHDDEKGEVRGRNTVFAIRLD
                     DMVICHLGDLGHALPAADLDRIGDVDIALVPISGPDTNLSAARAAEIVHQLEPKVVVP
                     MSYDPDDTKKDTPFQRLLHELGVKDLEPLPKLSVTRGTLPENLQVIALDSRAR"
     gene            1530..2090
                     /locus_tag="VFV20_04740"
     CDS             1530..2090
                     /locus_tag="VFV20_04740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5287687.1"
                     /translation="MLNRSDQEVVARCLSGEADAFSTLVERYGGRVYNIALRITNDAD
                     AAKDCAQEAFIRAYRALHQYDPALPFGPWLFRITTNASLNHVQRWHAHETPVEEFPDS
                     PEPDEAGPESSALRREELGEVLAAMAELPPHYRAALALRHMQQLSYQEVADSLGIPLG
                     TVKTHLHRARSALKSRLAARRKEREA"
BASE COUNT          345 a          699 c          759 g          299 t
ORIGIN      
        1 tgatgccggc gagcgtgagc agcgtcgcga tgcccggaag tgcctgggcg agctgctcgc
       61 ccagctgcgc ggtgagcagc aggctgaacg agaggatgag cccgccgacg aagagcagcg
      121 cgacctggat gaggccggcg acgctgcgcc ggccgccgcc ggtgaggagc cactcgatga
      181 cgccggcggc gacgagcacg agatagccgc cggtctgggc ggaggcatgc gcaccgacga
      241 agtcggtcgt ctggggtagc gcgttccccg tcccgaggat gacctggacg atcacgccga
      301 gcgtggagcc gatgacgagc gtggtgagcc cgaagatgac cgagagcttc gtgatgtcga
      361 gcctcgcgag accgccttcg gccatccccc gcgatgcggc gaaccaccac cagtagagga
      421 tgaccgcgag ctcggtgacg ccgaagaccg cgcgcgcctg gaagttgccg gaccagaacg
      481 cgatcacgta cgcggccgtc gatgccgcgc tgacgcgcac cgcggtgttc atgccggtgg
      541 cgccgaacag ccagatggtg accccgatca cgcccatcgt gatccagccg agcgtgccgg
      601 aatgcaggtg cgtgagcagg gtgttccggt cgagcgcgcc gaagagctgg gtcgcgttcg
      661 cgagaccgag gagcgcggtg agcgtgaaga tcgcgagccc gtactggaat gccgaacggg
      721 cagccgcctt tgtcatgtcg tccctcccgg cgccggaggg tacggaatgc gcgggccggc
      781 gtcgagatat aagagccgct gaccctaccg ggcgcgcgaa tcgagggcga tgacctgcag
      841 gttctccggg agcgttccgc gcgtgaccga gagcttcggc agcggctcca ggtccttcac
      901 ccccagctca tgcagcaggc gctggaaggg tgtgtccttc ttggtgtcgt ccgggtcgta
      961 cgacatcggg accaccacct tcggctcgag ctggtggacg atctccgccg cccgcgccgc
     1021 cgacaggttc gtgtccgggc cgctgatcgg gacgagcgcg atgtccacgt caccgatgcg
     1081 gtccaggtcc gcggcaggca gggcgtgtcc gagatcgccc aggtggcaga tgaccatgtc
     1141 gtccaggcgg atggcgaaga ccgtgttgcg cccccggacc tcgcccttct cgtcgtcgtg
     1201 gaagctcccg atgccggtca cgagcacgtc gtggacctcg tactctccgg gtccgagcag
     1261 gacgacgggc tctcctgcga cggagcggag gctggagtga ccggcgtggt cgtgggaaat
     1321 ggtcacgagg ccggcctcgg tgcgagggat cgcgtgtccg gacttcggat cgggtggatc
     1381 ggtgaggacg atgccctccc ggccgcgcat acggaagcaa gcgtggccga gccaggtcat
     1441 gtccatcggg agtccaacga tacgcgaaac cgcgcgcagc ggccgcgggt agtgctgccg
     1501 gagggggcgc gtcggcgcgg ggtcggcccg tgctgaatcg cagcgaccag gaggtcgtcg
     1561 cgaggtgcct ctccggcgaa gcggacgcct tttcgacgct ggtcgagcgg tacggaggac
     1621 gcgtgtacaa catcgcgctg cggatcacga atgacgccga tgcggcgaag gactgcgccc
     1681 aggaagcgtt catccgcgcc taccgcgccc tccaccaata cgaccccgct ctcccattcg
     1741 gaccgtggct cttccgcatc accaccaacg ccagcctcaa ccatgtccag cgctggcacg
     1801 cgcacgagac gccggtcgag gagttccccg actcgcccga gcccgacgag gccggccccg
     1861 agtcgtcggc gctgcggcgc gaggagctcg gcgaggtcct tgccgcgatg gccgagctgc
     1921 ccccgcacta ccgcgcggcg ctcgccctcc gtcacatgca gcagctctcg tatcaggagg
     1981 tggccgactc gctcggcatc cccttgggca ccgtgaagac gcacctgcat cgcgcgcgat
     2041 cggcgctgaa gagccggctc gcggcacgcc gaaaggagcg cgaggcatga gcgaccgctt
     2101 gg
//
LOCUS       DASTZR010000214         4667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_142762, whole genome shotgun sequence.
ACCESSION   DASTZR010000214 DASTZR010000000
VERSION     DASTZR010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142762"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..514
                     /gene="erpA"
                     /locus_tag="VFV20_04745"
     CDS             110..514
                     /gene="erpA"
                     /locus_tag="VFV20_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016328669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEU5287688.1"
                     /translation="MAIEQQQTSTEADPRVVSMTSRAADKLKEIIAKQGRDDLALRVY
                     VTPGGCSGFSYGMTFAEGREEDDTLIEQDGVRIVVDPMSAMYLKGSEIDFVDALMGGG
                     FALRNPNAVSSCGCGQSFRTAEQAGTAKACAH"
     gene            626..1162
                     /locus_tag="VFV20_04750"
     CDS             626..1162
                     /locus_tag="VFV20_04750"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEU5287689.1"
                     /translation="MSRDQRERRALLVVDMIHDFADPSGALYCGPSMERIIPVVASEI
                     ARARSSREPVVYLTDSHLTDDAEFKMFPPHAIAGTKGAEIIPQLAAAPGDSVIPKRRY
                     SGFFGTDLDITLRERGVTTLRMVGDCTNICVLYTAADARNLGYDVEVVREGVTSFDEE
                     AHRDALRELEKTLGATLV"
     gene            complement(1159..1512)
                     /locus_tag="VFV20_04755"
     CDS             complement(1159..1512)
                     /locus_tag="VFV20_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEU5287690.1"
                     /translation="MDITGAGLLVRIYIGEQDQWHGRPLYQAIVEFLREKRIAGATVL
                     RGIEGFGAHAHLHTTRLLRLSEDLPILIEIVDREDRLRSVLPELDAMVGGGLVTLERV
                     EVLTYRPPKDAAPPS"
     gene            complement(1512..1883)
                     /gene="crcB"
                     /locus_tag="VFV20_04760"
     CDS             complement(1512..1883)
                     /gene="crcB"
                     /locus_tag="VFV20_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU5287691.1"
                     /translation="MPYLLVGLGGFLGAIARYAVDGWITRWLGASFPFGTLVINASGS
                     LVIGILFAASVERATLPPDIRAPVMIGFIGAYTTFSTFMLESWRLFEDGAWLLGIANI
                     AGSAAIGLIAVGIGLAIGRAV"
     gene            complement(1893..2327)
                     /locus_tag="VFV20_04765"
     CDS             complement(1893..2327)
                     /locus_tag="VFV20_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287692.1"
                     /translation="MAGDPVLRRRAAVAEICGVAARTVIERVVDEAVAASSRFGLTRE
                     RANSYATGIETMLPAVLDTMRLPDSPERSAGIDRIAREVRAVSEQHRVPRIVERGLTA
                     IAIRIARELVRRRAADHGFGPDELEAEFLAFGDQLEARLFSG"
     gene            complement(2356..3099)
                     /locus_tag="VFV20_04770"
     CDS             complement(2356..3099)
                     /locus_tag="VFV20_04770"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU5287693.1"
                     /translation="MPTIRLPDPSEHGDMVKKIDTMIREISKAAAMTPSTRALGLRPA
                     ILMGNHQELAHVMATRTLLNLQKRMIALAVAATKSAPYELYAHTRGLQREYGLDDGQI
                     VEIVAVIAHVTSINTFERAIAAFNDVAPMRAQDPSAPILVEVRAKLGSVPRYFTYMAG
                     DPLYAKIVLEREISTIHEGEVSRLNKELIAYATSVVNDGKLSIAYRAESLRNLGMTNE
                     QLFEATTVISVFAKNCAFSTGLQLEPTAS"
     gene            complement(3392..>4667)
                     /locus_tag="VFV20_04775"
     CDS             complement(3392..>4667)
                     /locus_tag="VFV20_04775"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5287694.1"
                     /translation="DRALMEQARAKVRIAEGARTRILVVDDHVAVREGLRSLIGEYDD
                     LEIVGEAAGAEEGLRLARRHRPDVIVLDNEMPGRSGIDVVRELRDDVPLARIVMFSLD
                     GAARERAIEAGAHTFIVKDAPMDEILAALRPERTVAPASQRPGPVPRPAGQHRLRHAA
                     VVIAVAAGVYAAVFLIVESVFGAAAGAFAVIPVVMVAALLGSGAGAFAGALIIALNAF
                     LWTVTGHGVGESILTVGDPGFGMVILLLVGFGVGAIRDLRLLIDPRRARVETVAEAAR
                     AFAGIERGEVVGVYLEAVLRVVPADLAVLYAATGGEPRLVASSHALDAGMSERVAATV
                     RGAMRSPAARVIDEVPEADRPLARTRSALLAPVALPDHDLQGVLVLFSSHPFAAADLA
                     RARLFAQHLSVAMSNADLAPGRAAAHARERLS"
BASE COUNT          757 a         1606 c         1649 g          655 t
ORIGIN      
        1 cctcgccgtg gcggcaaggg aactgggcat gatggctgga aatggccacg gatcgagtgt
       61 aggtgcgcta gactcttcag cagagccttc agaaataggg gtgacgcacg tggcgatcga
      121 gcagcagcag accagcaccg aggcagatcc gcgggtcgtc tcgatgacct cgcgcgccgc
      181 cgacaagctc aaggagatca tcgcgaagca gggccgcgac gacctcgccc tgcgcgtcta
      241 cgtcacgccg gggggctgct cgggcttctc gtacggcatg accttcgcgg agggccgcga
      301 ggaggacgac accctcatcg agcaggacgg cgtccgcatc gtcgtcgacc cgatgagcgc
      361 gatgtacctc aagggatccg agatcgactt cgtcgacgcc ctcatgggcg gcggcttcgc
      421 gctccgcaac ccgaacgccg tgtcgagctg cggctgcggc cagtcgttcc gtacggcaga
      481 acaggccggc accgcgaagg cctgcgctca ctaatacggc cgcgagggag ggctttgccc
      541 gaccgagccg tctccggccc caggggcagg ggcccctgtt cagcgagcgc agcgagcgtg
      601 gaatgaagca gccgaacgcg gcgaaatgag cagggatcag agggaacgac gagctctgct
      661 cgtcgtggac atgatccacg acttcgcgga tccatccggt gcgctgtact gcgggccgtc
      721 catggagcgg atcatcccgg tcgtcgcgtc cgagatcgcg cgcgcgcgtt cgtcgcgcga
      781 gccggtcgtc tatcttaccg acagccacct gaccgatgac gccgagttca agatgttccc
      841 gccgcacgcc atcgccggga cgaaaggtgc ggagatcatc ccgcagctcg cggcggcgcc
      901 cggggacagc gtcatcccga agcgccgcta ctccggattc ttcggcacgg acctcgacat
      961 cacgctccgc gagcgcggcg tgaccacgct gcgcatggtc ggcgactgca ccaacatctg
     1021 cgtcctctac acggccgccg atgcgcgcaa cctgggctac gacgtggagg tcgtgcgcga
     1081 aggcgtcacg tcgttcgacg aggaggcgca ccgcgacgcg ctgcgggagc tggagaagac
     1141 gctcggcgcg acgctggtct aggagggcgg cgcggcgtcc ttgggcgggc ggtaggtcag
     1201 cacctccacg cgctcgagcg tgacgagacc gccgccgacc atcgcgtcga gctcgggcag
     1261 gacgctgcga agtcggtcct cgcggtccac gatctcgatg aggatgggca ggtcctcgga
     1321 cagccggagg agccgcgtgg tgtgcagatg ggcatgcgcg ccgaagccct cgatgccgcg
     1381 cagcaccgtg gctccggcga tgcgcttctc gcggaggaac tcgacgatgg cctggtacag
     1441 cggacgtcca tgccattgat cctgctcgcc gatgtagatg cgcacgagga gcccggcgcc
     1501 tgtgatgtcc atcagaccgc cctcccaatg gcgagaccga tgccgaccgc gatcagtccg
     1561 atcgcggccg acccggcgat gttcgcgatc ccgaggagcc acgcgccgtc ctcgaagagg
     1621 cgccacgact cgagcatgaa cgtcgagaag gtcgtgtagg ccccgatgaa gccgatcatg
     1681 accggcgcgc ggatgtcggg cggcagcgtc gcgcgctcga cgctcgccgc gaacaggatg
     1741 ccgatcacga gcgacccgct cgcgttgatg acgagcgtgc cgaacgggaa tgacgccccc
     1801 agccaccgcg tgatccagcc gtccacggcg tagcgcgcga tcgcgccgag gaagccgccc
     1861 agacccacga gcagataggg cacgccagca ggctacccgg agaacaacct cgcctcgagc
     1921 tggtcgccga acgcgaggaa ttcggcctcg agctcgtctg ggccgaagcc gtgatcggcg
     1981 gcacgtctgc gcaccagctc acgggcgatg cgtatcgcga tcgcggtgag ccctcgctcg
     2041 acgatgcgcg ggacgcggtg ctgctcggac acggcccgca cctcgcgcgc gatgcgatcg
     2101 atccccgcgg accgctcggg agagtccgga agacgcatcg tgtccaggac cgccggcagc
     2161 atcgtctcga tgcccgtcgc gtacgaattc gcacgctccc gggtgaggcc gaaacgactg
     2221 gaggccgcga cggcctcgtc caccacgcgc tcgatgaccg ttcgcgccgc cacgccacag
     2281 atctccgcca cggcagcccg gcgccgcagg acggggtcgc ccgccatgtc ggtcagatcg
     2341 gcaccaggcg gtacgtcacg acgcggtcgg ctcgagctgg agaccggtgg agaacgcgca
     2401 gttcttcgcg aacacgctga tcaccgtggt ggcctcgaac agctgctcgt tcgtcatgcc
     2461 gaggttgcgc aggctctccg cgcgatacgc gatcgagagc ttgccgtcgt tcacgacgct
     2521 cgtcgcatac gcgatgagct ccttgttcag ccgtgacacc tcaccctcgt ggatggtcga
     2581 gatctcgcgc tcgagcacga tcttggcgta gagcgggtcg ccggccatat aggtgaaata
     2641 ccgtgggacg ctgccgagct tcgcgcggac ctcgaccagg atcggcgcgg acggatcctg
     2701 cgcccgcatc ggagcgacgt cgttgaaggc cgcgatcgct cgttcgaacg tgttgatgct
     2761 ggtcacgtgc gcgatgaccg cgacgatctc gacgatctgg ccgtcgtcga ggccgtactc
     2821 gcgctggagg ccgcgggtgt gcgcgtagag ctcgtacggc gcggacttcg tcgccgcgac
     2881 cgcgagcgcg atcatgcgct tctggagatt gaggagcgtt cgggtggcca tgacgtgcgc
     2941 gagctcctga tggttgccca tgaggatggc cgggcgcagg cccagcgcgc gcgtcgaggg
     3001 cgtcatcgcg gcggccttgg agatctcgcg gatcatcgtg tcgatcttct tgaccatgtc
     3061 gccgtgctct gaagggtcgg gtagtcggat cgtcggcatc gttctccctc tctcttcccg
     3121 cacgcgtgct ggacgcgagt ctaggcgcgc gcccctcgcc cgagcggggt aggaccaacg
     3181 ttcatcaggg gttccctcac acgaggggcg acccgaccag gtgcgcgcgc gatgctcccc
     3241 accgtggagc catggctgcg agcggacgtc acgcacgagg agtgggtggc cgcgcggtta
     3301 caggcggcgg ccctgctgcg tgcggtggag aacggcgacg cggaggcggt gagattcatc
     3361 ctgcggcgag gcgccccgac ggacgacggc gctagctgag acgctcccga gcatgcgccg
     3421 ccgcgcgccc cggcgcgagg tccgcgttgc tcatcgcgac cgagaggtgc tgcgcgaaga
     3481 gacgagcgcg cgcgaggtcc gccgcggcga acgggtggct cgagaagagg acgagtactc
     3541 cctggagatc gtgatccggt agcgcgaccg gcgcgagcag tgccgaacgc gtgcgcgcga
     3601 gcggacgatc cgcctcgggc acctcatcga tcacgcgagc ggccggggat cgcatcgcgc
     3661 cacgcaccgt cgccgcgacc cgctcggaca tgcccgcatc aagggcatgg gacgatgcga
     3721 cgagccgggg ctccccgccc gtggccgcgt acagcacggc gaggtccgcc gggacgacgc
     3781 gcagcacggc ttcgaggtac acaccgacga cctcgccccg ctcgatgccg gcgaacgccc
     3841 gcgccgcttc cgcgaccgtc tcgacccgag ctcggcgcgg atcgatcagg aggcgcaggt
     3901 cgcgtatcgc tccgacgccg aagccgacga gcaggaggat gaccatcccg aagcccgggt
     3961 cgccgacggt gaggatcgat tcgccgacgc catggcccgt gaccgtccag aggaatgcgt
     4021 tgagggcgat gatgagcgcg ccggcgaagg cgccggcccc ggacccgagc agcgcggcga
     4081 ccatgacgac ggggatcacc gcgaacgcgc cggcggccgc gccgaacacg ctctcgacga
     4141 tcaggaagac ggccgcgtag acccccgcgg ccacggcgat gacgaccgcg gcatgccgca
     4201 gccggtgctg acccgcgggt cgcgggaccg gcccggggcg ctgcgacgcc ggcgccaccg
     4261 tccgctccgg gcggagcgcg gcgaggatct cgtccatcgg ggcgtccttc acgatgaagg
     4321 tgtgcgcgcc ggcctcgatc gcgcgctccc gcgccgcgcc gtcgaggctg aacatcacga
     4381 tccgcgcgag cggaacgtcg tcgcgcagct cgcggacgac gtcgatgccg ctgcggccgg
     4441 gcatctcgtt gtcgaggacg atgacgtcgg gcctgtggcg ccgcgcgagg cggagtccct
     4501 cctccgcgcc ggcggcctcc cccacgatct cgaggtcgtc atattcgccg atgagcgacc
     4561 gcaggccttc gcgcacggcg acgtgatcgt cgacgaccag gatccgcgtg cgcgcgccct
     4621 ccgcgatccg caccttcgcg cgcgcctgct ccatgagcgc tcggtcc
//
LOCUS       DASTZR010000215         6729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_586971, whole genome shotgun sequence.
ACCESSION   DASTZR010000215 DASTZR010000000
VERSION     DASTZR010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6729
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586971"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VFV20_04780"
     CDS             complement(<1..569)
                     /locus_tag="VFV20_04780"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5287695.1"
                     /translation="MTRAFAEVRRILAERARTRRNPFAHTEPETAVAILERLSSTEHA
                     PWVAAFMAEAEACAARRDWRGAYGYSRVARYPSLTSEEKRAAYVRSRDWYLRSVAERD
                     PATLRIEIPFAARAGEGLCVPAYLRVPRASAPRPVVVTWGGIDAFKEERRTDAFVAEG
                     WATLAIDMPGTGEAPISGAGDAERMWDAV"
     gene            complement(566..850)
                     /locus_tag="VFV20_04785"
     CDS             complement(566..850)
                     /locus_tag="VFV20_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287696.1"
                     /translation="MLTQLTRSASVMAVELREARMDVRASFPTAEKYGVGTGGSAMET
                     LTYVIDLATDAPRERVLAAVRSAEAACHAANSLRAPITASLRINGEEVAV"
     gene            complement(851..1102)
                     /locus_tag="VFV20_04790"
     CDS             complement(851..1102)
                     /locus_tag="VFV20_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287697.1"
                     /translation="MNDESEPLVDAARVRHEHEERARELAGHPERARITKRAVVRVVR
                     DYLKEARVGQFTFQSDEHAPAGGGAAPTPLDYFVAAVGL"
     gene            complement(1099..2238)
                     /locus_tag="VFV20_04795"
     CDS             complement(1099..2238)
                     /locus_tag="VFV20_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEU5287698.1"
                     /translation="MGQELFPEFSDAEIARRRGLVAELMREQDVECVVAYGAGKNAEA
                     YWLTNWPGTRESYLVWPRTGEPVLLVQLFNHVPNARRMARGAEVRWGGSTSATTLADL
                     LRERGMTRRVGVSGALPWRVADELRQKVAGLELVDLSAKVRALHLVKSAEELERLRIA
                     CRLTDRAMEALEREARPGMRESELEAIVECAYGREGRHGIHFMATTPMRSPEIGVPSQ
                     IQSWRRIAAGDVLITEISAEYWGYSGQIHRAYAIGADPTPEYQRIHDIAVETFERVCG
                     ALRDGATSDDVLDAAEVVHERGLTIYDDLLHGTNQLPPILKTRRTTHTPVKGFVFRED
                     MVVVVQPNVVREDARAGMQVGETVRITRDGVERLHSYPMRFVRCA"
     gene            2259..3275
                     /locus_tag="VFV20_04800"
     CDS             2259..3275
                     /locus_tag="VFV20_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU5287699.1"
                     /translation="MATETAPLALLRTMLRIRRLEERVIHFGRDHKLIRAHYHVYIGQ
                     EAAGTGACAALRRDDLVFTTHRNHGHVLAKGGEPAPIMAEILGRADGYQGGRAGTFHV
                     AAPDIGILHTSAIVGGCLPLAAGTAFARKRLGGDRVTVVFFGDGTMEEGVFYETLNIA
                     QLWRLPVLFYMENNSVSPQERSGRGSPTSEHSAKALSDVPRSFGVDTLVIDGTKVHTV
                     NETVSYLVGRMRRGEGPFFIESRTTRWPGNYGSLPRLPGGDTDIAWAWDTGRAPEIIR
                     AWHAQSDPVLIEARELLAAKAVDQDGLRALDLEVRDEIARAAERAIASPEPSAKDVYR
                     NVYA"
     gene            3268..4269
                     /locus_tag="VFV20_04805"
     CDS             3268..4269
                     /locus_tag="VFV20_04805"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEU5287700.1"
                     /translation="MPERTLKFAAAIAEALDGILAEDPSVLLIGARFVGITPSAAVME
                     PVTTKYADRIVWPPVAELAYCGIAIGAAMVGMRPIVELSTSTFSYEAIPQIVNEAPII
                     HANSGGRVNVPVTFHMLYGIRGAGAAQHSGSPQAWYYSAPGLQVVLPGSPADVKGLLR
                     WSALRSRNPTVFLDHQGLMEIEGPVPEGPYEIPFGQADVKRAGEDVTVVATSIQVPRS
                     LEAAELLAAEGISVEVVDPRTIEPLDRATILASVRKTKRAVVTDESPDRGGVVAGIAA
                     IIAEECHGDLVAPVRRVAVPTVPVPYAESLERELVPSVERIADAVRSVVGRRTAASR"
     gene            complement(4353..5069)
                     /locus_tag="VFV20_04810"
     CDS             complement(4353..5069)
                     /locus_tag="VFV20_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287701.1"
                     /translation="MKHLDKALLGAGAALLLSGGVAAAALGPTNVGGAVLSSLPGGIT
                     LNVAEHGPFGGPNVFATAATYIGISEADLRTELASGKSLADVAVAHGKTRDGLIAALV
                     QASTTQITTLVDTKDPFPAPPAGAPGGRGMHLMGGDDMAAAATYLGVTEADLRTKLQA
                     GQTLAQIAGATAGKSRDGLIQALVAAETAEIDQAVKDGKITADQATQVKANLTTRITQ
                     FVDNARPMGPGFGPGPRGHR"
     gene            5211..5891
                     /locus_tag="VFV20_04815"
     CDS             5211..5891
                     /locus_tag="VFV20_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5287702.1"
                     /translation="MHVLLVEDDPSVRGAVERALRGAGHEVELVTAGDTALDRATANP
                     YDAIVLDRGLPGLDGLEVCRRIRAGGSSVPILMLTARAAVSERVEGLDAGADDYLVKP
                     FALDELLARLRAFERRAPPAGQSRGALRFDDLEVDRDAMTARRGDREIPLSRTEYQLL
                     ELLAANPRRVLSRDVIFEKVWGYDFGPESNSLDVYIGYLRRKLEAGGEKRLIHTVRGV
                     GYVMREAA"
     gene            5888..>6729
                     /locus_tag="VFV20_04820"
     CDS             5888..>6729
                     /locus_tag="VFV20_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEU5287703.1"
                     /translation="MTFSVRVALLTALAVAVAAVGAAILMYFVVQNQFVAQIDQSLSE
                     AARSARQSGPRPGPRPFGDDPGRMAGRADVFAQSIDASGNILRADLNQPAKALVTAQA
                     MAIAASQHGDAWFDVIAADGEHVRVYVVPLGGNAALEVARSVDEIDEALAETRVRLAL
                     VALGGVLLAGALGAVVARTALRPVRRLTNIVEDVARTRDLSQRVATSSNDELGRLAAS
                     FNVMLGELETSLRQQRQLVADASHELRTPLTSLRTNLELLERGQPVDPAERQQVLADL
                     VGQM"
BASE COUNT          920 a         2429 c         2394 g          986 t
ORIGIN      
        1 aagacggcgt cccacatccg ctccgcgtcg cccgcgccgc tgatgggtgc ctcgccggtc
       61 ccgggcatgt cgatcgcgag cgtcgcccac ccctccgcga cgaaggcatc ggtgcggcgc
      121 tcctccttga acgcgtcgat cccgccccag gtgacgacga ccgggcgcgg cgcgctcgct
      181 cgcggcaccc gcaggtacgc gggcacgcac aggccctcgc cggcgcgcgc ggcgaagggg
      241 atctcgatgc gcagggtcgc cgggtcacgc tccgcgacgg agcgcaggta ccagtcgcgc
      301 gagcgaacgt acgccgcccg tttctcctcc gacgtcaggg acgggtaccg cgcaacgcgc
      361 gagtagccat acgcaccgcg ccagtcgcgg cgggccgcgc aggcctccgc ctccgccatg
      421 aacgcggcga cccacggcgc atgctcggtc gacgagagcc gttccaggat cgcgacggcg
      481 gtctcgggct cggtgtgcgc gaacggattg cggcgggtgc gggcgcgctc ggcgaggatg
      541 cggcggacct cggcgaacgc gcgcgtcaaa cagcgacctc ttccccattg atgcgcaggc
      601 tggcggtgat cggcgcccgc aggctgttcg cggcatgaca cgccgcctcg gcgctgcgga
      661 cggccgcgag cactcgctca cggggtgcgt ccgtcgcgag atcgatgacg tacgtgagcg
      721 tctccatcgc cgagccgccg gtcccgacgc cgtacttctc cgccgtcggg aacgacgcgc
      781 gcacgtccat gcgcgcctcg cggagctcga ccgccatgac cgacgccgac cgcgtcagct
      841 gggtgagcat tcagaggccg accgcggcca cgaaatagtc gagcggcgtc ggcgccgctc
      901 cgccgccggc gggcgcgtgc tcgtcgctct ggaacgtgaa ctgcccgacg cgcgcttcct
      961 tcaggtagtc gcgaacgacg cgcacgacgg cgcgcttggt gatccgcgcg cgctccggat
     1021 gaccggcgag ctcgcgtgcg cgctcttcgt gctcgtgccg caccctcgcg gcgtccacga
     1081 ggggttcgct ctcgtcgttc acgcgcagcg cacgaagcgc atcggatacg agtgcaggcg
     1141 ttcgacgccg tcgcgggtga tgcgcaccgt ctcgcccacc tgcatgccgg cgcgcgcgtc
     1201 ctcgcggacg acgttcggct gcacgacgac gaccatgtcc tcgcggaaga cgaagccctt
     1261 gaccggtgtg tgcgtggtgc gcctggtctt gaggatgggc gggagctgat tcgtcccatg
     1321 gaggaggtcg tcgtagatcg tgagcccgcg ctcgtggacg acctcggcgg cgtcgagcac
     1381 gtcgtcgctc gtcgcgccgt cgcggagcgc cccgcatacg cgctcgaacg tctcgaccgc
     1441 gatgtcgtgg atgcgctggt actccggcgt cggatcggcg ccgatcgcgt acgcgcggtg
     1501 gatctgtccg ctgtagcccc agtactcggc gctgatctcg gtgatgagga cgtcgcccgc
     1561 ggcgatgcgg cgccacgact ggatctgcga gggcacgccg atctccggcg agcgcatcgg
     1621 cgtcgtcgcc atgaagtgga tgccgtggcg gccctcgcgg ccgtacgcgc attcgacgat
     1681 ggcctcgagc tccgactcgc gcatgcctgg ccgcgcctcg cgttcgagcg cctccatggc
     1741 gcgatcggtg agccggcagg cgatgcgcag tcgttcgagc tcctccgcgc tcttcaccag
     1801 atgcagcgca cgcaccttcg cggacaggtc cacgagctcg aggcccgcga ccttctgccg
     1861 cagctcgtcg gccacgcgcc acggcagggc gccggacacg cccacgcgcc gcgtcatgcc
     1921 gcgctcgcgc agcaggtcgg ccagcgtcgt ggcgctggtc gagccgcccc agcgcacctc
     1981 ggcgccgcgc gccatgcggc gcgcgttggg gacgtggttg aagagctgca ccagcagcac
     2041 cggctcgccg gtgcgcggcc agacgaggta ggactcgcgc gtgcctggcc agttggtgag
     2101 ccagtacgcc tcggcgttct tcccggcgcc gtaggcgacg acgcattcga cgtcctgctc
     2161 gcgcatgagc tcggcgacca ggccgcggcg gcgggcgatc tccgcatcgc tgaattccgg
     2221 aaacagttcc tggcccactc gccgctagag tacccgcggt ggcgaccgag accgctcccc
     2281 tcgcattgct gcggacgatg ctgcgcatcc gccgcctcga ggagcgggtc atccacttcg
     2341 gccgagacca caagctgatc cgcgcgcact accacgtgta catcgggcag gaggccgcgg
     2401 gcaccggcgc gtgcgcggcc ctccggcgcg acgatctcgt cttcacgacg caccgcaacc
     2461 acggccacgt ccttgcgaag ggcggcgagc ccgcgccgat catggccgag atcctcggcc
     2521 gcgccgacgg ctatcagggc gggcgggccg gcacgttcca cgtcgccgca cccgacatcg
     2581 gcatcctgca cacgtccgcg atcgtcggcg gctgcctccc cctcgccgcg ggcaccgcgt
     2641 tcgcacggaa gcgccttggc ggcgaccgcg tcaccgtcgt gttcttcggc gacggcacca
     2701 tggaggaggg tgtcttctac gagacgctga acatcgcgca gctctggcgg ctgccggtcc
     2761 tcttctacat ggagaacaac agcgtcagcc cgcaggaacg gagcggtcgc ggctccccca
     2821 cctccgaaca cagcgcgaag gcgctgtcgg acgtgccgcg ctcgttcggc gtcgacacgc
     2881 tcgtcatcga cgggacgaag gtccacaccg tgaacgagac ggtgtcgtac ctcgtcgggc
     2941 ggatgcgccg cggcgaggga ccgttcttca tcgagtcgcg gacgacgcgc tggccgggca
     3001 attacggctc gctgccgcgc ctgccgggcg gagacaccga catcgcctgg gcctgggaca
     3061 cgggccgcgc acccgagatc atccgcgcgt ggcacgcgca gagcgacccc gtgctcatcg
     3121 aggcgcgcga gctcctcgcc gcgaaggctg tcgatcagga tgggctgcgt gcgctcgatc
     3181 tcgaggtgcg cgatgagatc gcccgcgcgg ccgagcgggc catcgcgagt cccgagccgt
     3241 ccgcgaagga cgtctaccgg aacgtgtatg cctgagcgca cgctgaagtt cgccgccgcg
     3301 atagcggagg cgctcgacgg gatcctcgcc gaggacccga gcgtcctgct catcggcgcg
     3361 cgcttcgtcg gcatcacgcc gagcgcggcg gtgatggagc ccgtgacgac gaagtacgcg
     3421 gaccgcatcg tgtggccacc ggtcgccgag ctcgcgtact gcggcatcgc catcggcgcg
     3481 gcgatggtgg gcatgcgccc gatcgtcgag ctgtccacgt cgacgttctc ctacgaggcc
     3541 atcccgcaga tcgtgaacga ggcaccgatc atccacgcga actccggcgg ccgcgtgaac
     3601 gtccccgtga cgttccacat gctctacggc atccgcggag ccggcgccgc gcagcactcg
     3661 ggcagcccgc aggcctggta ctacagcgcg ccgggactcc aggtcgtgtt gccgggcagc
     3721 cccgccgacg tgaagggtct cttgcgctgg tcggcgctgc gcagccgcaa tcccacggtg
     3781 ttcctcgacc accagggcct catggagatc gagggacccg tccccgaggg tccgtacgag
     3841 atcccgttcg gacaggccga cgtgaagcgc gccggcgagg acgtgacggt cgtcgcgacc
     3901 tcgatccagg tcccgcgctc cctcgaggcg gccgagcttc tcgcggcgga gggcatctca
     3961 gtggaggtcg tggatccgcg gacgatcgag ccgctcgacc gcgcgacgat cctcgcgtcg
     4021 gtgcgcaaga cgaagcgggc cgtcgtcacc gacgagagcc ccgaccgcgg cggcgtcgtc
     4081 gccggtatcg cggcgatcat cgcggaggag tgccacggcg acctcgtggc gccggtgcgc
     4141 cgcgtcgcgg tgcccaccgt gcctgtcccg tacgccgaat cgctcgagcg cgagctcgtg
     4201 ccgagcgttg agcggatcgc ggatgccgtc cgctccgtcg tgggtcgccg gacggcggcc
     4261 tcccgctaaa cacgtcgaac gggccgcgct cctcggtggg gttgaggagc gcggcccgtg
     4321 acgcaggagg gggtaagaga gggaggctct ggtcagcggt gtccgcgcgg acccggaccg
     4381 aagcccggac ccatcggacg cgcgttgtct acgaactgcg tgatccgcgt ggtgaggttc
     4441 gccttgacct gggtcgcctg gtccgcggtg atcttgccgt ccttgacggc ctggtcgatc
     4501 tccgccgtct cggccgccac gagggcctgg atgaggccgt cacggctctt gcccgccgtc
     4561 gcgccggcga tctgcgcgag ggtctggcct gcctgcagct tggtgcggag atccgcctcg
     4621 gtcacgccga gataggtcgc cgcggcggcc atgtcgtcgc cgcccatgag gtgcatgccg
     4681 cgtccgccgg gggcacccgc gggcggtgcc gggaacgggt ccttggtgtc gacgagcgtc
     4741 gtgatctgcg tcgtggacgc ctggaccagc gcggcgatga ggccgtcgcg ggtcttgccg
     4801 tgcgccacgg cgacgtccgc gaggctcttg ccgctcgcga gctcggtgcg caggtccgcc
     4861 tccgagatgc cgatgtacgt ggcggccgtc gcgaacacgt tcggtccgcc gaagggaccg
     4921 tgctccgcca cgttgagggt gatgccgccc gggagcgagc tgagcacggc gccgccgacg
     4981 ttcgtcggac cgagcgccgc cgcggccacg ccgccggaga gcaggagcgc cgcgcccgct
     5041 ccgaggaggg ccttgtcgag atgcttcatc tgcgtgattc ctttcgtgtg gatcgctacg
     5101 atcgaaggac accacgcccg gtccgtcgaa gcatgaaacg gccctaagaa ccggcggtcg
     5161 tcgtgcgcgc tcttagtcct ctcttaaccc gccgtccgta gactcgggac gtgcacgtcc
     5221 tccttgtgga ggacgatcca tcggtacggg gcgcggtcga gcgtgcgctg cgcggcgcgg
     5281 gccacgaggt ggagctggtg accgcgggcg acacggcgct cgaccgggcg accgcgaacc
     5341 cctacgacgc gatcgtcctc gaccgcggcc ttcccggcct cgacggcctc gaggtgtgcc
     5401 ggcgcatccg cgccggcggc agctccgtgc cgatcctcat gctcaccgcg cgcgccgccg
     5461 tctccgagcg cgtcgagggt ctcgatgccg gcgccgacga ctacctggtg aagccgttcg
     5521 cgctggacga gctgctcgca cggctgcgcg ccttcgagcg ccgcgcaccg ccggccggcc
     5581 agtcccgggg ggcgctgcgc ttcgacgacc tcgaggtcga ccgcgacgcc atgacggcgc
     5641 ggcgcggcga tcgcgagatc ccgctctcgc gcaccgagta ccagctgctc gagctgctcg
     5701 ccgccaatcc gcgtcgtgtc ctctcgcgcg acgtgatctt cgagaaggtg tggggctacg
     5761 acttcggccc cgagtcgaac tcgctcgacg tgtacatcgg ctacctgcga cgcaagctcg
     5821 aggccggcgg tgagaagcgg ctcatccaca ccgtgcgcgg cgtcggctac gtgatgcgcg
     5881 aggccgcatg acgttctccg tccgcgtcgc actgctcacc gcgctggcag tcgcggtcgc
     5941 agccgtcggc gcggcgatcc tcatgtactt cgtcgtgcag aaccagttcg tggcgcagat
     6001 cgaccagagc ctgagcgagg cagctcgcag cgcccgccag agcgggccgc gtccggggcc
     6061 gcgtccattc ggcgacgacc cgggccggat ggccggacgc gccgatgtgt tcgcgcagag
     6121 catcgatgcg agcggcaaca tcctccgcgc ggacctgaat cagccggcga aggcgctcgt
     6181 gacggcccag gcgatggcga tcgcggcatc gcagcacggg gacgcgtggt tcgacgtgat
     6241 cgctgcggat ggcgagcacg tgcgcgtcta cgtcgtgccg ctcggcggca acgccgcgct
     6301 cgaggtggcg cgctccgtgg acgagatcga cgaagccctc gccgagacac gcgtccgcct
     6361 tgcgctcgtc gcgctcggcg gcgtgctgct cgccggagcg ctcggtgctg tcgtcgcgcg
     6421 cacggcgctg cgaccggtcc ggcggctcac gaacatcgtc gaggatgtcg cgcgcacccg
     6481 cgacctgtcg cagcgggtcg cgaccagctc gaacgacgag ctcgggcggc tggccgcgtc
     6541 cttcaacgtg atgctcggcg agctcgagac gtcgctgcgg cagcagcgcc agctcgtcgc
     6601 cgacgcctcg cacgagctgc ggaccccgct caccagcctg cgcacgaacc tcgagctcct
     6661 cgagcgcggt cagccggtcg acccggcgga acggcaacag gtgctcgccg acctcgtggg
     6721 ccagatgga
//
LOCUS       DASTZR010000216         2876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_299542, whole genome shotgun sequence.
ACCESSION   DASTZR010000216 DASTZR010000000
VERSION     DASTZR010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299542"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1359)
                     /gene="sufB"
                     /locus_tag="VFV20_04825"
     CDS             complement(<1..1359)
                     /gene="sufB"
                     /locus_tag="VFV20_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEU5287704.1"
                     /translation="MATDVRKDIPDNYKYGWYDADAKYENVKKRGLDEAVIREISQTY
                     KKEPQWMFERRLKAFRHYLKRPMPDWGADLSKIDFDEIYYYLRPSGKTEKSWSDVPDY
                     VKRTFDKLGIPEAEQKYLAGVGAQYDSESVYHNIRKDLEAKGVLFMDTDTALREHEDI
                     MKQYFGTIIPANDNKLAALNTAVWSGGSFVWVPPGVNVEIPLQAYFRINSESAGQFER
                     TLIIAEEGSQVHYVEGCTAPVFSTATLHSAVVEIVVKKGARVRYTTIQNWSHNVYNLV
                     TKRAVVEADGVMEWIDCNLGSAVTMKYPSVYLVGERAHGEVLSVAFAADGQHQDAGAK
                     MVHAAPGTTSLITSKSISKGTGRTSYRGLVKIYPGMKRSKSTVRCDALILDPSARSDT
                     YPYMEIDEEDVSIGHEATVSKVGEEQLFYLMSRGLSEAEATAMVVNGFIEPIVKTLPM
                     DYA"
     gene            complement(1363..1761)
                     /locus_tag="VFV20_04830"
     CDS             complement(1363..1761)
                     /locus_tag="VFV20_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEU5287705.1"
                     /translation="MYRDYILDHYKNPRNSGELPGATNTYHDSNPLCGDEITMALLID
                     DDVVRDVRFQGKGCAISQASASILTEEIKGKTLDEVKAIDRQHILENLGIPISPARVK
                     CALLGLKTLKGAAWGLQEWPDEATAGKRTE"
     gene            complement(1775..>2876)
                     /locus_tag="VFV20_04835"
     CDS             complement(1775..>2876)
                     /locus_tag="VFV20_04835"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU5287706.1"
                     /translation="MREVFEEHNANIHRGVYEFSERTTAAFEGAREKVARFIGGEARE
                     IIFVRNATEGVNLVAYTWGREHIKAGGRIVSTVLEHHSNFVPWQQLAKDVGAEVLVID
                     IDDEGRLRRDQFTAALAKKPALVAITYTSNGLGTVLPIKELVAEAKSAGATVLVDAAQ
                     AVPHQRVDVRDLGCDFLVFSGHKMLAPPGSGAVWGRAALLEQMRPFLFGGDMIASVTV
                     ERTTWNELPWKFEAGTSSYVDAIGLGAAVDYLESVGMEKIHQHELSLVGYLLPRLEEI
                     PGVTVYGPKTLDDRVGVVSFNIEGIHPHDAASIFDREGVCVRAGHHCNQLLMARLSVP
                     ATTRASFYLYNTPQECDTLIGAIHSAKKIFGV"
BASE COUNT          407 a          960 c          954 g          555 t
ORIGIN      
        1 cgcgtagtcc atcggcagcg tcttcacgat cggctcgatg aaaccgttca cgaccatcgc
       61 cgtcgcctcg gcctcgctga ggccccggct catgaggtag aagagctgct cctcgccgac
      121 cttcgagacg gtggcctcgt ggccgatcga gacgtcctct tcgtcgatct ccatgtacgg
      181 gtaggtgtcg ctgcgcgcgg acgggtcgag gatgagcgcg tcgcagcgca cggtgctctt
      241 cgagcgcttc atgcccgggt agatcttcac caggccgcgg tacgacgtgc ggccggtgcc
      301 cttgctgatg gacttgctcg tgatgaggct cgtggtgccg ggagcggcgt ggaccatctt
      361 cgcgccggcg tcctggtgct ggccgtcggc cgcgaacgcg accgagagca cctcaccgtg
      421 ggcgcgctcg ccgacgaggt acacgctcgg gtacttcatc gtgaccgccg agccgaggtt
      481 gcagtcgatc cactccatga cgccgtctgc ctcgacgacc gcgcgcttcg tcacgaggtt
      541 gtagacgttg tggctccagt tctggatcgt cgtgtagcgc acgcgcgcgc ccttcttcac
      601 gacgatctcg acgaccgcgc tgtgcagggt cgcggtactg aagaccggcg ccgtgcatcc
      661 ctcgacgtag tgcacctgcg agccttcctc cgcgatgatc agcgtgcgct cgaactggcc
      721 cgcgctctcg ctgttgatgc ggaagtacgc ctggagcggg atctcgacgt tcactcccgg
      781 cgggacccac acgaacgagc cgcccgacca caccgcggtg ttcagcgcgg cgagcttgtt
      841 gtcgttcgcg gggatgatcg tgccgaagta ctgcttcatg atgtcctcgt gctcgcggag
      901 ggccgtgtcg gtgtccatga agaggacgcc cttcgcctcg aggtccttgc ggatgttgtg
      961 gtacacggac tcgctgtcgt actgcgcgcc gacgccggcg aggtacttct gctcggcctc
     1021 ggggatcccg agcttgtcga acgtgcgctt cacgtagtcc ggcacgtcgg accagctctt
     1081 ctcggtcttc ccggacgggc gcaggtagta gtagatctcg tcgaagtcga tctttgacag
     1141 gtcggcgccc cagtcgggca tcggccgctt caggtagtgg cggaacgcct tgagccggcg
     1201 ttcgaacatc cactgcggct ccttcttgta ggtctggctg atctcgcgga tgacggcctc
     1261 gtcgaggccg cgcttcttca cgttctcgta cttcgcgtcg gcgtcgtacc agccgtactt
     1321 gtagttgtcg gggatgtcct tgcggacgtc tgtcgccatc gcctactccg ttctcttgcc
     1381 cgcggtggcc tcgtccggcc actcctgcag gccccatgcg gcgcccttga gcgtcttgag
     1441 tcccagcagc gcgcacttca cgcgcgccgg gctgatcggg atgccgagat tctcgaggat
     1501 gtgctggcgg tcgatcgcct tcacctcgtc gagcgtcttg cccttgatct cctcagtgag
     1561 gatggatgcg gatgcctgcg agatcgcgca gcccttgccc tggaagcgca cgtccctcac
     1621 cacgtcgtcg tcgatcagga gcgccatggt gatctcgtcg ccgcagagcg gattgctgtc
     1681 gtggtacgtg ttcgtcgcgc cgggcagctc acccgagttc cgcgggttct tgtagtggtc
     1741 gaggatgtag tcgcggtaca tgtcgtccat ggtcctagac gccgaagatc ttcttcgccg
     1801 aatggatcgc gccgatgagc gtgtcgcact cctgcggcgt gttgtacagg tagaaggacg
     1861 cgcgcgtcgt ggcgggcacg gaaagccgcg ccatgagcag ctggttgcag tggtgcccgg
     1921 cgcggacgca cacgccctcg cggtcgaaga tcgacgccgc gtcgtgcggg tggatgccct
     1981 cgatgttgaa cgacacgacg cccacgcggt cgtcgagcgt cttcggcccg tagaccgtga
     2041 cacccgggat ctcctcgagg cgcggcagca gatagccgac gagcgagagc tcgtgctggt
     2101 ggatcttctc catcccgacg ctctcgaggt agtcgaccgc cgcgccgagc ccgatggcat
     2161 cgacgtacga ggacgtgccg gcctcgaact tccacggcag ctcgttccac gtcgtccgct
     2221 ccacggtcac ggacgcgatc atgtcgccgc cgaagaggaa cgggcgcatc tgttcgagca
     2281 gcgccgcgcg gccccagacc gcacccgagc cggggggcgc gagcatcttg tgcccgctga
     2341 agacgaggaa gtcgcagccc aggtcgcgca cgtccacgcg ctgatgcggc acggcctgcg
     2401 ccgcgtccac cagcacggtg gcgccggcgg acttcgcctc cgcgacgagc tccttgatcg
     2461 ggagcaccgt gccgagtccg ttggacgtgt aggtgatcgc cacgagcgcg ggcttcttcg
     2521 cgagcgcggc ggtgaactgg tcgcgccgca gccggccctc gtcgtcgatg tcgatcacga
     2581 gcacctcggc gcccacgtcc ttcgcgagct gctgccacgg cacgaagttc gaatggtgct
     2641 cgaggaccgt gctcacgatc cggccgccgg ccttgatgtg ctcgcggccc caggtgtagg
     2701 cgacgaggtt cacgccctcg gtcgcgttgc ggacgaagat gatctcgcgc gcctcgccgc
     2761 cgatgaaccg cgcgaccttc tcgcgcgcgc cctcgaaggc cgcggtggtg cgctcgctga
     2821 actcgtagac gccgcgatgg atgttcgcgt tgtgctcctc gaacacctca cgcatc
//
LOCUS       DASTZR010000217         2390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_401485, whole genome shotgun sequence.
ACCESSION   DASTZR010000217 DASTZR010000000
VERSION     DASTZR010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401485"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1353
                     /locus_tag="VFV20_04840"
     CDS             <1..1353
                     /locus_tag="VFV20_04840"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU5287707.1"
                     /translation="QLDVRDAATIDQALGLIAAHARDLPAGAWILGGRFDKNVWGRWP
                     TAQELDHASAGHPAALRSRDGHSRWLNSDALRRVGIGPDTKAPEGGTILRDASGAPMG
                     ILQENANNLVDAVVPPPTDDEMLEAARRGQREAWKRGLTGFENLDAFRDRFTLATFER
                     LRDAGELAMRAHIGIPRVAVDDAIARGLRTGQGDDWLRVGHLKIFTDGALGSQTAALE
                     EPYLGSDDRGVLTIEPAALTRLVGRAAEAGIAVAIHAIGDRAVHTALDAIEPTRRTAP
                     ALRPKVEHVQLVRADDLGRFASLGIVPSMQPIHATSDRDLADRYWGPERVARAYPWRT
                     LLASGARLAFGSDAPVEPIDPLLGIHAAVARRRPGDADAWTPAQRLTLDEALAGYAAG
                     AAYALGRESDLGTLAEGMWCDATVVDRDLARASEDELLAARVRATVTGGVVRYAEGLG
                     "
     gene            1350..>2390
                     /locus_tag="VFV20_04845"
     CDS             1350..>2390
                     /locus_tag="VFV20_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU5287708.1"
                     /translation="MIQLVLHGELRQWSPGGRKQLSIPAAGRRAGDVVRELGIPAGET
                     AAYVVNGEQREADVVLADGDTLEVLPAISGGASADGALAGVRVLDLTRALAGPYCTLM
                     LGDHGADVIKVEMPGVGDETRSWMPPAMNGVSAYYLSINRNKRSVTIDLKNAEGAKVV
                     ERLMERCDVVVENFSPGTLARLGFPDDRIRAINRRAIVCHVSGFGQDGPGRAWPAYDL
                     IVQGMGGIMSLTGEVGNDPVMVGVPQADIVAGMFAAFAITAALHARERTGEGQVIDAT
                     MIGGQVALLSRQAARYFADGTVPRPEGNVHASIVPYQTFRASDGHVNVGVANNALWER
                     FCRALDLEELLED"
BASE COUNT          343 a          842 c          890 g          315 t
ORIGIN      
        1 cagctcgatg tgcgtgacgc ggcgacgatc gaccaggcgc tcgggctcat cgctgcgcac
       61 gcgcgggacc ttcccgcggg tgcatggatc ctgggaggcc ggttcgacaa gaacgtctgg
      121 ggacgctggc cgacggcgca ggagctcgac cacgcgagcg cggggcatcc ggcggcgctg
      181 cgcagccgcg acggtcactc ccgctggctc aacagcgacg cgctacgacg cgtcggcatc
      241 ggccccgaca cgaaggcgcc ggagggcggc acgatcctgc gtgacgcatc gggagcgccg
      301 atgggcatcc tccaggagaa cgcgaacaac ctcgtcgacg ccgtggtgcc cccgccgacc
      361 gacgacgaga tgctcgaggc cgcccgccgc gggcagcgcg aggcgtggaa gcgggggctg
      421 accgggttcg agaacctcga cgcatttcgc gatcgattca cgctcgcgac cttcgagcgc
      481 ctgcgcgacg cgggcgagct cgcgatgcgc gcccacatcg gcatcccacg cgtcgcggtc
      541 gatgacgcga tcgcgcgcgg gctgcgcacg ggccagggcg acgactggct gcgcgtcggg
      601 cacctcaaga tcttcaccga cggcgcgctc ggttcgcaga ccgccgcgct cgaggagccg
      661 tacctcggga gcgatgaccg gggcgtcctc accatcgagc ccgccgcgct cacgcggctg
      721 gtcggtcggg ccgcggaggc cggcatcgcg gtggcgatcc acgcgatcgg cgaccgcgcc
      781 gtgcacaccg cgctcgatgc gatcgagccg acgcgccgga cggcgcccgc gctccgtccg
      841 aaggtcgagc acgtccagct ggtgcgcgcg gacgatctcg gccgtttcgc cagcctcggg
      901 atcgtcccgt cgatgcagcc gatccacgcg acgagcgatc gcgacctcgc cgatcgctac
      961 tggggacccg agcgcgtcgc gcgcgcgtac ccctggcgca cgctcctcgc aagcggtgca
     1021 cggctcgcgt tcggctccga cgcgcccgta gagccgatcg atccgctcct cggcatccac
     1081 gcggcggtcg cgcgccgcag gcccggcgac gccgatgcgt ggaccccagc tcagcgcctg
     1141 acgctcgacg aggcgctcgc gggctacgcc gcgggtgccg cgtacgcgct cggtcgggag
     1201 agcgatctcg ggacgctcgc ggagggcatg tggtgcgacg cgaccgtcgt ggatcgcgac
     1261 ctggcccgcg cgtcggagga tgagctcctc gcggcgcgcg tccgcgcgac cgtgaccggg
     1321 ggcgtcgtgc ggtacgccga gggcctcggg tgatccagct cgtgctccac ggcgagcttc
     1381 gccagtggag tcccggtgga cggaagcagc tgtcgatccc ggcggcggga cgccgcgccg
     1441 gggacgtggt ccgcgagctc gggatcccag cgggcgagac cgcggcttat gtggtgaacg
     1501 gtgagcagcg cgaggcagac gtcgtcctcg cggacggcga cacgctcgag gtgctgccgg
     1561 cgatcagcgg cggcgcgtcg gctgacggcg cgctcgcggg cgtccgcgtg ctcgacctga
     1621 cgcgcgcgct cgcgggcccc tactgcacgc tcatgctcgg cgaccacggc gccgacgtca
     1681 tcaaggtcga gatgccgggc gtcggggacg agacgcgctc gtggatgccg ccggcgatga
     1741 acggggtctc cgcgtactac ctctcgatca accgcaacaa gcgcagcgtc accatcgacc
     1801 tgaagaacgc cgaaggcgcg aaggtggtcg agcgactcat ggagcgctgt gacgtggtgg
     1861 tcgagaactt cagcccggga acgctcgcgc ggctcggctt ccccgacgat cgcatccgcg
     1921 ccatcaaccg ccgcgcgatc gtgtgccacg tgagcggctt cgggcaggac ggccctggac
     1981 gcgcatggcc tgcgtacgac ctcatcgtcc agggcatggg cgggatcatg agcctcacgg
     2041 gcgaggtcgg gaacgatccg gtgatggtgg gcgtgccgca ggccgacatc gtcgcgggga
     2101 tgttcgcggc gttcgccatc accgccgcgc tgcacgcgcg ggagcggacc ggcgaggggc
     2161 aggtgatcga cgcgacgatg atcggcggcc aggtcgcgct gctctcgcgt caggccgcac
     2221 ggtacttcgc cgacggcacg gtcccgcgac ccgaggggaa cgtgcacgcc tcgatcgtcc
     2281 cgtatcagac gttccgtgca tcggacgggc acgtgaacgt cggcgtggcg aacaacgcgc
     2341 tgtgggagcg cttctgccgt gcgctcgacc ttgaggagct cctcgaagac
//
LOCUS       DASTZR010000218         6619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_456299, whole genome shotgun sequence.
ACCESSION   DASTZR010000218 DASTZR010000000
VERSION     DASTZR010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6619
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456299"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VFV20_04850"
     CDS             complement(<1..211)
                     /locus_tag="VFV20_04850"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5287709.1"
                     /translation="MRFTYRDEQLQVRDTVREFARARIAPNADAWSDAGEFPMALLPE
                     LAALGLTGLAVPTELGGAGLDTLTIA"
     gene            complement(211..612)
                     /locus_tag="VFV20_04855"
     CDS             complement(211..612)
                     /locus_tag="VFV20_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287710.1"
                     /translation="MAIFGLGAKKDTRPVDVGLASLLGKSDAEAVEFWKGRLIQISQI
                     PSDVARVGALTPQLRELSRVEDAGERRRLTHARILAFAQLTPDQQRLLAESRRKAFSV
                     DAGVMDSDQKFVDEILPTVDESVRRVYPRRP"
     gene            complement(725..1144)
                     /locus_tag="VFV20_04860"
     CDS             complement(725..1144)
                     /locus_tag="VFV20_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013288515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEU5287711.1"
                     /translation="MKRPIRVVVAKAGLDGHDRGAKVVARALRDAGMEVIYTGVHLTP
                     EQVVETALQEDADAVGISLLSGAHNYIFPRISELMKEKGMSDVLLFGGGIIPEADIPG
                     LAKVGVERVFPPGASLDEIVRYVNEAVGKRRGEPVRG"
     gene            complement(1141..2871)
                     /locus_tag="VFV20_04865"
     CDS             complement(1141..2871)
                     /locus_tag="VFV20_04865"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEU5287712.1"
                     /translation="MSTETLGTSSDLLARVEHVYVSTRSVIDALPAERFDERLPSGMT
                     LREVVAHLAAWEETVPPRVEHALATGSDLGRHEDIDVFNANVFAETRDATVDDLRARL
                     ARSHAAVVEIVRSFEHREVPKLALDIVEWNTTGHYPDHFGDFGAAIKDANDLVAVVQQ
                     GWTTFRLAVMSLGVSGLERTSPAGWTYKAMVAHCAGWERLTVTRLRTLREAGTFELSG
                     VQTDEFNAKMAEDAASRDVRDVLEDLQTAHAELVAEIGKLSPQQIHANDDWAIAVVAG
                     NSYGHYGEHHAELFAAVPTRPAELLELMREGWRSFRGTLSRVGLLPLSHTTSAGWTAK
                     ALLAHLAYWLESVGASLPERLAGRRGRVPDIEAENARERAGADARTAHEIVGRLDRAY
                     REVVRLVEALPPDEDIHFMAVRLIAGETYGHFAGHLRELAPFIPTTTAGVLRRFDESW
                     SALRARVREVGRAGLGDTTPAGWTYRDCLAHLANWMQNAASELESGASPAWNAETIQA
                     ENDRAVAAHRLVGPEAMLDELDSSHRRVRDVIAGLSDERIRDRRVFDVVAFYTYLAWE
                     EHFEELGITV"
     gene            complement(2843..4525)
                     /locus_tag="VFV20_04870"
     CDS             complement(2843..4525)
                     /locus_tag="VFV20_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015237114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEU5287713.1"
                     /translation="MTLQKDRSGKAEWRERVYDPAVKKRAERKQSFTTISGDPVEPLY
                     TADDLEGFDPARDLGAPGEFPYTRGIHPTMYRSRLWTMRQFAGFGSAEDTNKRFHYLL
                     KHGVTGLSTAFDMPTLMGRDADDPLSRGEVGREGVAVSTMRDMEILFDKIPVDEVTTS
                     MTINGPASIVWAMYLGAAEKRGYPLAALGGTIQNDCLKEYIAQREWIVPERAAMGLVV
                     DTFRFGAREVPKWNTISVSGYHIREAGATALQELAFTLIDGLEYVKWGVEAGLDVNDF
                     VPRISFFFDVHNDFFEEIAKFRAARRIWAREIEKRYHPTNPRALQLRTHAQTAGVSLT
                     AQQPYNNVVRVALQALAAVLGGTQSLHTNSLDETYALPTEEAVTLALRTQQVIAHESG
                     VADTVDPLAGSYYLEHLTGRMEDGFARYLREIESMGGMVDAVERGYPQREITEASFRF
                     QEQVEQKEKLIVGINAYVTEQEPPIPVLQIDPEVERRQILRLADTRRTRDAGRWREAM
                     ARLREAATAAKDDKRAELMPAFLDCARAYASVGEQVQVLKEVFGVYRDPGYF"
     gene            complement(4522..5697)
                     /locus_tag="VFV20_04875"
     CDS             complement(4522..5697)
                     /locus_tag="VFV20_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5287714.1"
                     /translation="MQLVPSATDRELALAVRGWATGLAGKIADAERQGALPPGVIREL
                     GELGVLGMTTPERDGGLGASTLAFTLVLEELAAAWPSLAVGVSVNSGIVAGSIVRYGT
                     AEQRARWLPLLRDGGGLGAFALTEPTSGSDAASLRATAVRDGDGWRISGRKQFITNAR
                     YAPLFIVLARVGAAEEGVASRPGAPSPAHAGISAFVVPRDVPGVSVGPAESKTGLLAS
                     DTSALVLEDARVAGDAQLGEVGKGFGIAMAGLDGGRIGIAAQSVGIARAAVERAVGYG
                     RDRSQFGRSILSFEMVRFSLARSRMDLDAARLLAQRAAWLRDAGRPFTREASMAKLFA
                     SEAAQRVTYDAVQVFGGYGFMREYEVERYARDARATTIYEGTSEVQRMVIARSLLAA"
     gene            complement(5704..6582)
                     /locus_tag="VFV20_04880"
     CDS             complement(5704..6582)
                     /locus_tag="VFV20_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEU5287715.1"
                     /translation="MKVLVVAERKDGQVRRVSSEIAAKAKSFGEVEVVGVKGDRYSPM
                     AWTAAVAEKAKGADLVLVGATLNGRDLGARLAAKLGWAYAADCTDVTPKDGGIEAKRP
                     MYAGKIRAVVKVPTPAVISVRAGAWTLPADGAAPSITEIDAGPADERLAFGRFEATAS
                     GGGRVTLAEARAVVSAGRGLKGPENWKLVEGLADALGAAIGASRAVTDAGWRPNEEQV
                     GQTGKTVTPDLYVALGISGAIQHLAGMTSSKVIVAVNKDPDADIFKVADYGVVGDLFE
                     FVPAFTEAVKKVKASG"
BASE COUNT          948 a         2396 c         2271 g         1004 t
ORIGIN      
        1 tcgcgatggt gagcgtgtcg agcccggcgc caccgagctc cgtaggcacc gcgagaccgg
       61 tgaggccgag cgcggcgagc tccggcagga gcgccatcgg gaactcgccg gcatcgctcc
      121 aggcgtcggc gttcggagcg atgcgtgccc gcgcgaattc gcggacagtg tcgcggacct
      181 gcagctgttc gtcgcggtac gtgaaccgca ctatgggcgt ctcggataga cgcggcggac
      241 gctctcgtcc accgtcggca ggatctcgtc gacgaacttc tggtcggagt ccatcacgcc
      301 ggcatcgacc gagaatgcct tccgtcgcga ctctgcgagg agccgctgct ggtcgggcgt
      361 gagctgcgcg aacgcgagga tgcgcgcgtg tgtgaggcgg cgccgctcgc ccgcgtcctc
      421 gacgcgcgac agctcgcgca gctgtggcgt gagcgcgccg acgcgcgcca cgtcgctggg
      481 gatctgcgag atctggatga gccggccctt ccagaactcg accgcctccg cgtcgctctt
      541 gccgagcagg gatgcgaggc cgacgtccac cggccgggtg tccttcttcg cgccaagacc
      601 aaagatcgcc atgcgggcct ccttttcttg cgagcgccgg tgatcgttcc ggcgctcgtc
      661 atcccgcacc ttccctgcct caccgccgac cggcgaccgt gccaccggac cgccgtcgaa
      721 accgctagcc acggaccggc tcgccgcgtc gcttgccgac cgcctcattc acgtaccgca
      781 cgatctcgtc caacgacgcg ccgggcggga aaacgcgctc aacgccgacc ttcgcgagcc
      841 cggggatgtc cgcctcggga atgatgccgc cgccgaagag gagcacgtcg gacatgccct
      901 tctccttcat gagctccgag atcctcggga agatgtagtt gtgcgcgccg gagaggaggc
      961 tgatgccgac ggcgtcggcg tcctcctgca gcgccgtctc cacgacctgc tcgggcgtga
     1021 ggtgcacgcc cgtatagatc acctccatgc cggcatcgcg cagcgctcgc gccacgacct
     1081 tcgcgccccg gtcgtgccca tcgaggccgg ccttcgccac gacgacccgg atcggccgtt
     1141 tcataccgtg atccccaatt cttcgaaatg ctcttcccac gcgagatacg tgtagaaagc
     1201 cacgacgtcg aacacgcgcc ggtcgcggat gcgctcgtcc gagagcccgg cgatcacgtc
     1261 gcgaacgcgc cggtgcgagc tgtcgagctc gtccagcatg gcctcggggc cgacgagccg
     1321 gtgcgcggcg accgcgcggt cgttctcggc ctggatcgtc tccgcgttcc acgcggggga
     1381 ggcgccgctc tcgagctcgc tcgcggcgtt ctgcatccag ttcgcgagat gcgcgaggca
     1441 atcgcggtag gtccagcccg ccggcgtcgt gtcgcccagc ccggcgcgcc cgacctcgcg
     1501 gacgcgtgcg cgcaacgctg accacgactc gtcgaagcgg cgcagcacgc ccgcggtggt
     1561 cgtcgggatg aacggcgcga gctcgcggag gtgacccgcg aagtgcccat acgtctcgcc
     1621 ggcgatcagg cgcaccgcca tgaagtggat gtcctcgtcc ggcgggagtg cctccacgag
     1681 gcgcacgacc tcgcgatagg cgcggtcgag tcgcccgacg atctcgtgcg ccgtgcgcgc
     1741 gtcggcgccg gcacgctcgc gcgcgttctc ggcctcgatg tccggcaccc ggccgcggcg
     1801 ccccgcaagg cgttcgggca gtgacgcccc gacgctctcg agccagtacg cgaggtgcgc
     1861 gaggagcgcc ttcgccgtcc agcccgcgga cgtggtgtgc gacagcggaa gcagtcccac
     1921 acggctgagc gtgccgcgga acgagcgcca gccctcgcgc atgagctcca gcagctcggc
     1981 cggccgcgtg ggcaccgccg cgaagagctc ggcgtggtgt tcgccgtaat ggccgtagct
     2041 gttgcccgcc acgacggcga tggcccagtc gtcattcgcg tggatctgct ggggtgacag
     2101 cttcccgatc tcggcgacga gctcggcgtg cgccgtctga aggtcctcga gcacgtcgcg
     2161 cacgtcccgc gacgccgcgt cctccgccat cttcgcgttg aactcgtcgg tctggacacc
     2221 cgagagctcg aacgtgccgg cctcgcgcag cgtccgcagc cgcgtcaccg tgaggcgttc
     2281 ccacccggcg cagtgcgcca ccatcgcctt gtaggtccac cccgccggcg acgtgcgttc
     2341 gagacccgag acgccgagcg acatcaccgc cagccggaac gtggtccacc cctgctgcac
     2401 gaccgcgacg aggtcgttcg cgtccttgat ggcggcacca aagtcgccga agtggtcggg
     2461 atagtgcccg gtcgtgttcc attcgacgat gtcgagcgcg agcttcggca cctcgcggtg
     2521 ctcgaacgac cgcacgatct cgacgaccgc cgcgtgcgac cgcgcgaggc gcgcgcgcag
     2581 atcgtccacg gtcgcgtcac gcgtctcggc gaacacgttg gcgttgaaca cgtcgatgtc
     2641 ctcgtggcgt ccgaggtcgc tgccggtggc gagcgcgtgc tcgacgcgcg gcggcacggt
     2701 ctcctcccac gcggcgagat gggcgacgac ctcgcgcagc gtcatgccgg atgggagccg
     2761 ctcgtcgaag cgctccgcgg ggagcgcgtc gatcaccgag cgcgtcgaca cgtacacgtg
     2821 ctcgacgcgc gcgagcaggt cgctagaagt acccagggtc tcggtagaca ccgaagacct
     2881 ccttcaggac ctgtacctgc tcgcccacgc tcgcgtaggc gcgcgcgcaa tcgaggaagg
     2941 ccggcatgag ctcggcacgc ttatcgtcct tcgccgccgt cgcggcttcg cgcagccgcg
     3001 ccatggcctc gcgccagcgt cccgcgtcgc gggtgcggcg ggtgtcggca aggcgcagga
     3061 tctggcgccg ttcgacctcg gggtcgatct gcagcacagg gatgggaggc tcctgctcgg
     3121 tcacgtacgc gttgatcccg acgatgagct tctccttctg ctcgacctgc tcctggaagc
     3181 ggaaggacgc ttcggtgatc tcgcgctggg ggtacccgcg ctcgaccgca tccaccatcc
     3241 cgcccatcga ctcgatctcg cgcaggtagc gggcgaagcc gtcctccatg cgaccggtga
     3301 ggtgctcgag gtagtaggag cccgcgagcg ggtcgacggt gtcggcgaca ccggattcgt
     3361 gcgcgatgac ctgctgcgtg cggagcgcga gcgttaccgc ctcttccgtc ggcagcgcgt
     3421 atgtctcgtc gagcgaattg gtgtgcagcg actgcgtgcc gccgaggacc gccgccagcg
     3481 cctgcagcgc cacacggacg acgttgttgt acggctgctg cgcggtgagc gagacgccgg
     3541 cggtctgcgc atgcgtgcgc aactgcaggg cccgcgggtt cgtcgggtgg tagcgcttct
     3601 cgatctcgcg cgcccagatg cggcgggccg cgcggaactt cgcgatctcc tcgaagaagt
     3661 cgttgtggac gtcgaagaag aacgagatgc gcggcacgaa gtcgttcacg tcgagaccgg
     3721 cctcgacgcc ccacttcacg tactccaggc cgtcgatgag cgtgaaggcg agctcctgga
     3781 gcgcggtcgc cccggcctcg cggatgtggt agccggagac cgagatggtg ttccacttcg
     3841 ggacctcgcg cgcgccgaat cggaacgtgt ccacgacgag ccccatggcc gcgcgctcgg
     3901 gcacgatcca ctcgcgctgc gcgatgtact ccttgaggca gtcgttctgg atggtcccgc
     3961 cgagcgcggc gagcggatag ccgcgcttct ccgcggcgcc caggtacatg gcccacacga
     4021 tcgacgccgg accgttgatg gtcatcgacg tcgtcacctc gtcgacgggg atcttgtcga
     4081 agaggatctc catgtcgcgc atcgtcgaca cggcgacgcc ctcgcgaccg acctcgccgc
     4141 gcgagagcgg gtcgtccgcg tcgcggccca tgagcgtcgg catgtcgaac gccgtcgaga
     4201 gcccggtcac gccgtgcttc agcaggtagt ggaagcgctt gttggtgtcc tccgcggatc
     4261 cgaagcccgc gaactggcgc atcgtccaga gacggctccg gtacatcgtg gggtggatgc
     4321 cgcgcgtgta ggggaactcg cccggtgcgc cgagatcgcg cgcggggtcg aagccctcga
     4381 ggtcatccgc cgtgtagagc ggctcgaccg ggtcgcccga gatcgtcgtg aaggactgct
     4441 tccgctcggc gcgcttcttc acggcggggt cgtacacgcg ctcgcgccat tccgccttgc
     4501 cggaccggtc cttctggagg gtcatgcggc cagcagcgag cgcgcgatga ccatgcgctg
     4561 cacctcgctc gtcccttcgt agatcgtcgt cgcgcgcgca tcgcgcgcgt agcgctcgac
     4621 ctcgtactcg cgcatgaagc cgtagccccc gaacacctgc acggcgtcgt acgtcacgcg
     4681 ctgcgcggcc tcgctggcga acagcttggc catcgacgcc tcgcgcgtga acggccggcc
     4741 cgcatcgcgc agccatgccg cgcgctgcgc gagcaggcgc gccgcatcga ggtccatgcg
     4801 tgagcgtgcc agcgagaatc ggaccatctc gaacgagagg atggaccgtc cgaactggct
     4861 gcggtcgcga ccgtagccga ccgcacgctc cacggcggcc cgggcaatgc cgacggactg
     4921 cgcggcgatc ccgatgcgcc cgccgtcgag gcccgccatc gcgatcccga agcccttccc
     4981 cacctcgccg agctgggcgt cgccggcgac gcgagcgtcc tccagcacga gcgcgctcgt
     5041 gtcggacgcg aggaggccgg tcttcgattc ggccgggccg acgctcacgc ccggaacgtc
     5101 gcgcgggacg acgaacgcgc tgatgccggc gtgtgcaggg ctaggggccc ctgggcgcga
     5161 agcgaccccc tcttccgcgg cgccgacccg agcgagcacg atgaagagcg gtgcgtagcg
     5221 cgcgttggtg atgaactgct tacgtccgga gatgcgccag ccgtcgccgt cgcggacggc
     5281 cgtcgcgcgc agcgacgcgg cgtcgcttcc cgaggtgggc tcggtgagcg cgaacgcgcc
     5341 gagcccgccg ccatcgcgca gcagcggaag ccagcgcgcg cgctgctccg ccgtgccgta
     5401 gcggacgatc gagccggcga cgatgccgct gttcacggac acgccgaccg cgagcgacgg
     5461 ccatgccgcc gcgagctcct cgagcacgag cgtgaacgcg agggtggacg cgcccaggcc
     5521 cccatcgcgc tcgggcgtcg tcatgccgag cacgccgagc tcgccgagct cgcggatgac
     5581 gccaggcggc agcgcgccct gacgctcggc gtcggcgatc ttgcctgcga gaccggtggc
     5641 ccagccgcgc accgcgagcg cgagctcccg gtcggtggcc gacgggacga gctgcatcag
     5701 cgcctaccca ctcgccttga ccttcttgac ggcctccgtg aaggcgggga cgaactcgaa
     5761 gagatcgccc accaccccgt agtccgcgac cttgaagatg tcggcatcgg ggtccttgtt
     5821 gaccgcgacg atcaccttgg atgaggtcat gcccgcgagg tgctggatcg cgccggagat
     5881 gccgagcgcg acatacagat caggcgtgac ggtcttgccc gtctgaccca cctgctcctc
     5941 gttgggacgc cagccggcgt cggtcaccgc ccgcgatgcg ccgatggcgg cgcccagcgc
     6001 gtcggcgagc ccctcgacga gcttccagtt ctccggaccc ttgaggccgc ggcctgcgga
     6061 cacgaccgcg cgcgcctcgg cgagtgtcac gcgaccgccg ccgctggcgg tggcctcgaa
     6121 gcggccgaac gcaaggcgct cgtcggccgg accggcgtcg atctccgtga tgctcggcgc
     6181 cgcgccgtcg gcgggaagcg tccacgcacc ggcgcgcacc gagatgaccg ccggcgtcgg
     6241 gaccttcacc accgcgcgga tcttgccggc gtacatcggg cgcttcgcct cgatgccgcc
     6301 gtccttcggc gtcacgtcgg tgcagtccgc cgcgtacgcc cagccgagct tcgccgcgag
     6361 acgcgcgccg aggtcacggc cattcagcgt cgcgccgacc agcacgagat cggcgccctt
     6421 tgctttctcg gcgaccgccg ccgtccacgc catgggcgaa tagcgatcgc ccttgacgcc
     6481 gacgacctcg acctcgccga agctcttcgc cttcgccgcg atctcgctcg agacgcggcg
     6541 gacctgaccg tccttccgct cagcgacgac aaggactttc ataggacctt cgcctcctcg
     6601 tgcagcgcct tcgccacct
//
LOCUS       DASTZR010000219         2083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_192409, whole genome shotgun sequence.
ACCESSION   DASTZR010000219 DASTZR010000000
VERSION     DASTZR010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192409"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VFV20_04885"
     CDS             complement(<1..188)
                     /locus_tag="VFV20_04885"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU5287716.1"
                     /translation="MRVVVALLAAGVLTASCAAGQPAPTASGGARVIEITMKEFGFTP
                     ATIQLRAGEKVTLRFKNVG"
     gene            480..>2083
                     /locus_tag="VFV20_04890"
     CDS             480..>2083
                     /locus_tag="VFV20_04890"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEU5287717.1"
                     /translation="MADVALLIDWENVYYTLRQHTTGPLPSSLAILQAILKKAAEFGP
                     VRVKLAIFGQEVASQDETLLMALEFTGIEPLAVAQRMSGRLLKGRSDAVLITRAMKLL
                     YKERPDIDVWFIVSGDRDLNALCKALKDEDKTVYLVAGDLSLANELRDSPYLRDGVFL
                     LEDLIPEARWTRGGLRADDTQVGKEPRRDEAPARAPALGGRTRRGGRGRGAAAAVTAK
                     PPVVPAVVPTGTPESEKEQRRLAVLLLDQLVAIRADAMPRSDFVQSVVPLSEKEASQV
                     LEQRIDAGVEQQHVVARSQGRFRAKAKQLIAPNVTSALVAETLFHLVRLIRRIDTVTK
                     DTKRVPVVEAVLDPLSRADQPGGLARGRSQRRVLLETLYNIAEERGALSSESVQRDGK
                     EITMCWLVDQHPLVQYARHPSAGIVHLFNFVGTTKGHGEKVDWVNQALFAELLSRVEG
                     DALESNLRAAVDRALMRPEAHGSKPGYVVDRAHDEVRMLLGEFHLLDGEGVAARPAPD
                     VSGVTEDAETDAPEPDEAAEPGGAIPA"
BASE COUNT          329 a          727 c          737 g          290 t
ORIGIN      
        1 ccgacgttct tgaagcgcag cgtgaccttt tcgccggctc ggagctggat cgtcgcgggg
       61 gtgaatccga actccttcat cgtgatctcg atcacgcgcg cgccaccgct cgctgtcggc
      121 gcgggctgac ccgcggcgca cgaggcggtg aggacgccgg cggcgagcag ggcgacgacg
      181 acacgcaccg cggcagcgta tggggcggcg cggaacgcgg acgcggaccg attcgcccct
      241 ctcgcggggt cgaaggtccc gttccgtcgc gttcggccca gcgcctggcc gagcgtccga
      301 tacactgacg acagcgaaaa acggcgcgcg cgacgacagg atgcgttcgt gatgacgcgc
      361 tcgcggccga cgtatcgggt gaccggtgcg aagcggacga gggtgagcat ctagcgcgtg
      421 actccgaaag gggtccgcga acgggtcagg cggcgtgcga gcccatatgg agaaccctat
      481 tggctgacgt cgctctcctc atcgactggg agaacgtcta ctacacgctg cgacagcaca
      541 ccaccggacc actgccgtcg tcgctcgcga tcctgcaggc catcctcaag aaggccgcgg
      601 aattcggacc ggtacgcgtc aagctcgcca tcttcggaca ggaggtcgcg tcacaggacg
      661 agacgctcct gatggcgctc gagttcaccg gcatcgagcc gctcgccgtc gcgcagcgca
      721 tgagcggccg gctgctcaag ggcagatccg acgccgtcct catcacccgc gcgatgaagc
      781 tcctgtacaa ggagcggccg gacatcgacg tctggttcat cgtctcgggc gaccgcgatc
      841 tgaacgcgct ctgcaaggcg ctgaaggacg aggacaagac cgtatatctc gtggcgggcg
      901 acctctcgct cgcgaacgag ctccgcgact cgccctacct gcgcgacggc gtgttcctcc
      961 tcgaggacct catccccgag gcgcgttgga cgcgcggcgg gcttcgtgcc gacgacacgc
     1021 aggtcggcaa agagccgaga cgcgacgaag cacccgcgcg cgcgcccgcc ctcggtgggc
     1081 gcacgcggcg cggcggccgg ggccgcggcg ctgccgcggc ggtgacggcg aagccgccgg
     1141 tcgtccccgc ggtcgtgccg accggcacgc cggagtccga gaaggagcag cggcggctcg
     1201 ccgtcctgct gctggaccag ctcgtggcca tccgcgccga cgcgatgcct cgctccgatt
     1261 tcgtgcagtc cgtggtcccg ctctccgaga aggaggcctc gcaggtcctc gagcagcgga
     1321 tcgacgccgg ggtcgagcag cagcacgtcg tcgcgcgctc gcagggccgc ttccgcgcca
     1381 aggcgaagca gctcatcgct ccgaacgtga cgtccgcgct cgtcgcggag acgctcttcc
     1441 atctcgtgcg cctgatccgc cgcatcgaca cggtgaccaa ggacacgaag cgcgtccccg
     1501 tcgtggaagc ggtcctcgac cccctgtcgc gtgcggacca gccgggcggg ctggcccgcg
     1561 ggcgctccca gcggcgcgtg ctgctggaga cgctctacaa catcgcggag gagcgcgggg
     1621 cgctctcgag cgaatccgtc cagcgtgacg gcaaggagat cacgatgtgc tggctcgtgg
     1681 accagcaccc gcttgtgcaa tacgcgcggc acccttcggc gggcatcgtc cacctcttca
     1741 acttcgtcgg gacgacgaag gggcatggcg agaaggtcga ctgggtgaac caggcgctct
     1801 tcgccgagct cctgtcgcgg gtcgagggtg acgcgctcga gtcgaacctg agggccgccg
     1861 tcgaccgcgc gctcatgcgt cccgaggcgc acggcagcaa gcccggctac gtcgtggacc
     1921 gcgcgcacga cgaggtgcgc atgctgctgg gcgagttcca tctgctcgac ggcgaaggcg
     1981 tcgctgcgcg ccccgcgccg gacgtgagtg gggtcaccga ggatgcggag accgacgcac
     2041 cggagccgga cgaggccgcc gagcccggcg gcgcgatccc cgc
//
LOCUS       DASTZR010000220         2603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1004621, whole genome shotgun sequence.
ACCESSION   DASTZR010000220 DASTZR010000000
VERSION     DASTZR010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004621"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VFV20_04895"
     CDS             <1..301
                     /locus_tag="VFV20_04895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEU5287718.1"
                     /translation="RRGTAPPIGTLRTWEELFEVLPRPIVVAWEGEHDLRLRDGLPTD
                     VPALSLVIGPEGGLTDEEIALAREHDAIVVSLGQRNLRAETASIAAVAIAMDLLD"
     gene            complement(454..1119)
                     /locus_tag="VFV20_04900"
     CDS             complement(454..1119)
                     /locus_tag="VFV20_04900"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU5287719.1"
                     /translation="MTLGAIAVSYVASSLSFPWLIARLYGVDLRRVGARKLGGSNLGK
                     TVGLAQGIVGGVLDGAKGFAAVLGARALDLPLETQLLCGIAAVAGQMWPVFHGFDGGR
                     ANATGWGFSIAADPVAALIMGSPIYAALAANAFVRPRPTRLLPLAGLLSFAIFPAVIW
                     EQEGMTPTALAGLAVLALVVLRRVTAGVRDDVATGAPLARIIANRALFDRSELQQRGE
                     VAI"
     gene            1138..1473
                     /locus_tag="VFV20_04905"
     CDS             1138..1473
                     /locus_tag="VFV20_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEU5287720.1"
                     /translation="MADCIMCRIVAGEIPARLVHQDERAIAFHDINPAAPFHVLVVPR
                     VHVARLADLEDAALAAHLIDVARRVAKEGGHAENFRLVVNNGDRAGQSVWHLHLHVLG
                     GRQFSWPPG"
     gene            1599..1766
                     /gene="rpsU"
                     /locus_tag="VFV20_04910"
     CDS             1599..1766
                     /gene="rpsU"
                     /locus_tag="VFV20_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEU5287721.1"
                     /translation="MSEVIVGEGESFEAALRRFNKKIQQAGILAEARRREFYERPAAR
                     RKRKEAKRRKR"
     gene            1849..2343
                     /locus_tag="VFV20_04915"
     CDS             1849..2343
                     /locus_tag="VFV20_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEU5287722.1"
                     /translation="MRAKDTLRRDTLRFLLAAVQREGVDRLGATVERLIAEGKDETAR
                     AAYLKEHDPSTLDDAAVSEVIQKQAKMRRDSIDAFRKGGRAELAAKEESELGILTEYL
                     PAQLTEAEVRAIVKRVVDETGAAGPRDMGKVMPKVLAETKDRADGKQVASIVQATLKE
                     KAGT"
     gene            2340..>2603
                     /locus_tag="VFV20_04920"
     CDS             2340..>2603
                     /locus_tag="VFV20_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287723.1"
                     /translation="MSSPAQITILIPDPDEMMFVTGTNDTNIDRIEREFAVKIVSRGA
                     ELRIMGEPANVARVGELVSAMRTLSDRDENLRKPALERLIGDAK"
BASE COUNT          437 a          883 c          945 g          338 t
ORIGIN      
        1 tcggcgcggg accgcgccgc ccatcggcac gctgcgcacc tgggaggagc tgttcgaggt
       61 cctgccacgt ccgatcgtcg tggcgtggga gggcgagcac gacctgcgac tgcgcgacgg
      121 cctgccgacc gatgtgccgg cgctgtccct cgtgatcgga ccggagggcg gcctcaccga
      181 tgaggagatc gcgctcgcgc gcgaacacga cgcgatcgtc gtgagcctcg gccagcggaa
      241 ccttcgcgcg gagaccgcgt cgatcgcggc ggtcgcgatc gcgatggacc tgctcgacta
      301 gctcctcacc acagccggtg cgctcgcccg gccgcgaaga agccgacgca gctgccggag
      361 aggaacgcca gcaggctgat cgccgggaag tacaggtcca cggactcccg caagagcacg
      421 cccgataccc gttaccggac cgttacgtgg tcctcagatg gccacctcgc ctcgttgctg
      481 gagctcgctg cggtcgaaga gcgcgcggtt cgcgatgatg cgggccagtg gcgcgcccgt
      541 ggcgacgtcg tcgcgaacgc cggcggtgac gcgtcgcagg acgacgaggg cgagcacggc
      601 gaggccggcg agggcggtgg gcgtcatgcc ctcctgctcc cagatcaccg ccgggaagat
      661 cgcgaaggag agcaggccgg cgagcggcag gaggcgcgtc gggcgcggac ggacgaacgc
      721 gttcgcggcg agcgccgcgt agatcgggct gcccatgatg agcgcggcga ccggatccgc
      781 cgcgatcgag aagccccagc cggtcgcgtt ggcgcggccg ccgtcgaacc cgtggaagac
      841 gggccacatc tgtccggcca ccgcggcgat gccgcagagg agctgcgtct cgagcgggag
      901 atccagcgcg cgtgccccga ggaccgcggc gaaccccttc gccccatcga gcacgccgcc
      961 gacgatgccc tgcgccaggc cgacggtctt gccgagattg ctcccgccga gcttgcgcgc
     1021 gcccacccgg cgcaggtcga cgccgtagag cctggcgatc agccacggga acgagaggct
     1081 gctggcgacg tagctgaccg cgatggcgcc gagggtcacg tcgctattct cacgaccatg
     1141 gcggattgca tcatgtgccg gatcgtcgcg ggcgagatcc cagcgcgtct ggtccaccag
     1201 gatgagaggg cgatcgcctt ccacgacatc aaccccgcgg cgccgttcca cgtgctcgtc
     1261 gtgccacgcg tgcacgtggc caggctcgcg gacctcgagg acgccgccct cgccgcgcat
     1321 ctcatcgacg tggcgcgccg cgtcgcgaag gagggcgggc acgctgagaa cttccggctc
     1381 gtcgtgaaca acggcgaccg cgccgggcag tccgtctggc acctgcacct gcacgtgctg
     1441 ggcggacggc agttctcctg gccgccgggc tgatccggct ggtaatatgc gtatctttca
     1501 cgtgcgccgc acggtcgccg gcggctatga ttcggacgtc ggccaccctc gggggccgac
     1561 gtgtcttgtg accagaaagg gcgggtgata ccaggtagtt gtctgaagtg atcgtcggcg
     1621 agggcgagtc gttcgaggcc gcgcttcgcc gcttcaacaa gaagatccag caggcaggga
     1681 tcctcgcgga ggcccgccgc cgcgagttct acgagcgccc cgccgcgcgc cgcaagcgca
     1741 aggaagccaa gcggcgcaag cgctagtaga cagcgtgagc gaccgagtag gagcttaggc
     1801 acgccctcag ctctcaagac gcggctcgag gaagacctga gggccgcgat gcgcgcgaag
     1861 gacacgctgc gtcgtgacac gctgcgcttc ctcctggccg ccgtgcagcg cgagggcgtg
     1921 gaccgccttg gggcgacggt ggagcggctc atcgccgagg gcaaggacga gacggcacgc
     1981 gccgcgtatc tcaaggagca cgatccgagc acgctcgacg acgccgcggt gtccgaggtc
     2041 atccagaagc aggcgaagat gcggcgcgac tccatcgacg ccttccggaa gggcgggcga
     2101 gcggagctcg cggcgaagga agagtcggag ctcggcatcc tcaccgagta cctcccggcg
     2161 cagctcaccg aggccgaggt ccgcgcgatc gtgaagcgcg tcgtcgacga gaccggcgcg
     2221 gccgggccgc gcgacatggg caaggtgatg ccgaaggtgc tcgccgagac gaaggaccgg
     2281 gccgacggga agcaggtcgc ctccatcgtc caggcgacgc tgaaggagaa ggccggcacg
     2341 tgagctcgcc cgcgcagatc acgatcctca tccccgatcc cgacgagatg atgttcgtca
     2401 ccgggaccaa cgacacgaac atcgaccgca tcgagcgcga gttcgcggtg aagatcgtct
     2461 cgcgcggcgc ggagctgcgg atcatgggcg agcccgcgaa tgtcgcgcgc gtcggggagc
     2521 tcgtcagcgc gatgcgcacg ctttccgatc gcgacgagaa cctccgcaag cccgcgctcg
     2581 agcggctcat cggtgacgcg aag
//
LOCUS       DASTZR010000221         3219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1161408, whole genome shotgun sequence.
ACCESSION   DASTZR010000221 DASTZR010000000
VERSION     DASTZR010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161408"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VFV20_04925"
     CDS             complement(<1..368)
                     /locus_tag="VFV20_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEU5287724.1"
                     /translation="MATVYYDKDADPRALKGKTIAVIGYGSQGHAHAQNLRDSGYDVI
                     VGLQEGSKSWAKAERDGFTVRETRDAACNADVIAILAPDQHHRAIYEKSVKPCLRKGT
                     TLVVAHAFSVHFKEIEPTPD"
     gene            complement(380..2164)
                     /locus_tag="VFV20_04930"
     CDS             complement(380..2164)
                     /locus_tag="VFV20_04930"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU5287725.1"
                     /translation="MAATFERATRERPAALPQAESGNAAVVDILRSWGITYFAGVNGG
                     GVVHVTKQMEPFGDLSQAKDGVSRMLTMSEYVAGFAPLGHWLASGRIGGCVVTTGAAT
                     KLGASGMSDAKLHNIPAVYLVALNSTMSIGNAPLQDVSEHGMNIVPQLEAELGEGCIV
                     IDNINRLEDGMKKAQHVLKRRKPVAIAFHPDVLARPTEAAVPWTSNPRTFSVRDVADF
                     LEEFPQVARGKRVIVYASAEAALVPGIQELTSQLSQLLRAPTVWSVNGASAVSPANPW
                     GHGYISFGGNDAAMKLWRGITSDDVVITLGFDPGEYSLNLGRIPAGYVYHFTDLRDPY
                     GHKQGEFRHRAANEYRIVRGDIGMVLREVLPQLARSIGKRPDTQPAPASLNDREISRS
                     VRPGTVDAVEFYGRLHQMWRPGSIGFDDVCTAYKDRQYVAQRPSEAIRFYTMHDGSAM
                     GGGFGLGVGAKCAEPDLHTFVFTGDGCWRLFGGALADAANLGMNVFVVNNGVYGIVDK
                     GLEVIIPDVDKRKYHAKLPQIDFVKAAEAHGWEAARVAPDLSNLRDIVDACYARDGRS
                     FLVEVPIDADQLIGLNPRLYNLTTKTYL"
     gene            complement(2412..3038)
                     /locus_tag="VFV20_04935"
     CDS             complement(2412..3038)
                     /locus_tag="VFV20_04935"
                     /inference="COORDINATES: protein motif:HMM:NF024945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5287726.1"
                     /translation="MLAGSARSAALRDAVAAAVDRDARVLRTTAGMTAVCEVAEGADP
                     RAEAERVRLAVADALNDDQMTAGVAGPKNGASGAHFALVQAEHALVVGRALHGPGRTT
                     HFDELGPYCFVLNQPAHEVHAFAERILGELITDDAHAELVDTLEAYLRTHGNLNAVAR
                     QLFLHRNTVRHRLRRIAKLTGADLDDPDARLGLQLAILGQRALSRVAS"
BASE COUNT          467 a         1062 c         1139 g          551 t
ORIGIN      
        1 atgtccggcg tcggctcgat ctccttgaag tgaacggaga aggcgtgcgc gaccacgagc
       61 gtcgttccct tgcgcaggca cggcttcacg ctcttctcgt agatcgcgcg gtggtgctgg
      121 tcgggggcga ggatcgcgat gacgtcggcg ttgcacgcgg cgtccctcgt ctcgcgcacg
      181 gtgaagccgt cgcgctcggc cttcgcccag gacttcgagc cctcctggag cccgacgatc
      241 acgtcgtagc cggagtcacg caggttctgc gcgtgggcgt gcccctggct gccgtatccg
      301 atgacggcga tggtcttgcc cttcagggcc ctggggtcgg cgtccttgtc gtaatacacg
      361 gtggccatcg tcgcctcctt tagagatagg tcttcgtggt caggttgtag agacgcgggt
      421 tgagaccgat gagctggtcg gcgtcgatcg ggacctcgac caggaacgac cggccgtcgc
      481 gggcgtagca cgcgtcgacg atgtcgcgca ggttcgagag gtccggcgcg acgcgcgcgg
      541 cctcccagcc gtgcgcctcc gccgctttca cgaagtcgat ctgcgggagc ttcgcgtggt
      601 acttgcgctt gtcgacgtcg gggatgatca cctcgaggcc cttgtcgacg atcccgtaca
      661 cgccgttgtt cacgacgaag acgttcatgc cgaggttcgc ggcgtccgcg agcgcgccgc
      721 cgaagaggcg ccagcagccg tcgccggtga agacgaacgt gtgcaggtcc ggctcggcgc
      781 acttcgcacc gacgccgagg ccgaagccgc cgcccatcgc ggagccgtcg tgcatggtgt
      841 agaagcggat ggcctcggag ggccgctgcg cgacgtactg gcggtccttg tacgcggtgc
      901 agacgtcgtc gaatccgatc gagccgggcc gccacatctg gtgcagccga ccgtagaact
      961 cgaccgcgtc gaccgtgccg ggccgcacgc tccgcgagat ctcgcggtca ttcaggctcg
     1021 ccggcgcggg ctgcgtgtcg gggcgcttgc cgatgctgcg ggcgagctgc ggcagcactt
     1081 cgcgcaggac catgccgatg tcgccgcgca cgatgcggta ctcgttcgcc gcacggtggc
     1141 ggaactcgcc ctgcttgtgc ccgtacgggt cgcgcaggtc ggtgaagtgg tagacgtagc
     1201 cggccgggat ccggccgagg ttcagcgagt actcgcccgg atcgaagccg agcgtgatca
     1261 cgacgtcgtc cgaggtgatc ccgcgccaca gcttcatcgc ggcgtcgttg ccgccgaacg
     1321 agatgtagcc atgcccccac gggttggccg gtgagacggc ggaagctccg ttcaccgacc
     1381 acaccgtcgg ggcacgcagg agttgagaga gttgggatgt cagttcctgg atgccgggga
     1441 ccagcgcggc ctcggcggac gcgtacacga tcacgcgctt gccgcgcgcg acctgcggga
     1501 actcctcgag gaagtcggcg acgtcgcgca ccgagaaggt gcgcgggttc gaggtccacg
     1561 gcaccgcggc ctcggtggga cgcgcgagga cgtcgggatg gaaggcgatc gcgaccggct
     1621 tgcggcgctt cagcacgtgc tgcgccttct tcatgccgtc ctcgagccgg ttgatgttgt
     1681 cgatgacgat gcagccctcg ccgagctcgg cctcgagctg cgggacgatg ttcatgccgt
     1741 gctcggagac gtcctgcagc ggcgcgttgc cgatcgacat cgtcgagttc agcgcgacga
     1801 ggtagacggc ggggatgttg tgcagcttcg cgtcggacat gccgctcgcg ccgagcttcg
     1861 tcgcggcgcc ggtcgtgacg acgcagccgc cgatgcggcc cgacgcgagc cagtgcccga
     1921 gcggcgcgaa gccggcgacg tactcgctca tcgtgagcat gcgcgagacg ccgtccttcg
     1981 cctgcgagag atcgccaaag ggctccatct gtttggtgac gtgcacgacg ccaccgccgt
     2041 tcacgccggc gaagtacgtg atgccccacg agcgcaggat gtcaacgacg gcggcgttcc
     2101 cgctctcggc ctgtggtagt gcggccggac gctcgcgtgt cgctcgttcg aatgtcgccg
     2161 ccatcgccta ctccttttcc cgttctgtct tgcgttcgct ggaactgaaa aggcctccct
     2221 gttcgggagg ccttcggtgc ttcgcttgat gcgctgtgtc tgccgctatc ctcccgcccg
     2281 gacatgcgcc ctctcgacga ggacgatgcc aataaggacg aggacggcgc gcagagctgc
     2341 catgtgcgcg tcagtgaacc cgccgcacca tcggagggtc aaggacgtgc gggctgtttg
     2401 tgcagcgtgc atcaggacgc cactcgcgag agggcgcgct gtccgaggat cgcgagctgc
     2461 aatccgagac gcgcgtcggg atcgtcgaga tcggcgccgg tgagcttcgc gatgcggcgc
     2521 agacgatgac gcaccgtgtt gcgatgcagg aagagctgac gagcgacggc gttgaggttg
     2581 ccgtgcgtgc gcaggtacgc ctcgagcgtg tccaccagct ccgcgtgcgc atcgtcggtg
     2641 atgagctcgc cgaggatgcg ctccgcgaat gcgtgcacct cgtgcgccgg ctgattgagg
     2701 acgaagcagt aggggccgag ctcgtcgaag tgcgtcgtgc gccccgggcc gtggagcgcg
     2761 cggcccacga cgagcgcgtg ctcggcctgc acgagcgcga agtgcgcgcc ggacgctccg
     2821 ttcttcggac cggcgacgcc cgcggtcatc tggtcgtcgt tcagcgcgtc cgcgaccgcg
     2881 agacggacgc gctcggcttc ggcgcgcgga tcggcgccct cggccacctc gcagaccgcg
     2941 gtcatgcccg ccgtcgtgcg cagcacgcgt gcgtcacgat cgacggcggc cgcgaccgca
     3001 tcgcgcagcg ccgcggaacg ggcgctgccc gcgagcacga tcgcgatgta cctgctgccg
     3061 ctcgtctcgt gacctcctca taacgaagaa ggcctccctt gtcgggaggc cttcggttcg
     3121 atcttggttc gcttcgcggg tcctagtcgc cgcctcccaa cgcctcttgc cccgtcacaa
     3181 ggacgaggat cgggataacg acgacgatga gccgctgca
//
LOCUS       DASTZR010000222         2300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1109558, whole genome shotgun sequence.
ACCESSION   DASTZR010000222 DASTZR010000000
VERSION     DASTZR010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109558"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VFV20_04940"
     CDS             <1..277
                     /locus_tag="VFV20_04940"
                     /inference="COORDINATES: protein motif:HMM:NF014908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU5287727.1"
                     /translation="EELARRVRGCAPDPGAFTTYRGQRLVVARASVTGGAPKEHGVVE
                     VRAGVPHVASGAGWLRLDEVRPAGKRTMSGAEWARGVRELDGARLPS"
     gene            312..1400
                     /gene="rlmN"
                     /locus_tag="VFV20_04945"
     CDS             312..1400
                     /gene="rlmN"
                     /locus_tag="VFV20_04945"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEU5287728.1"
                     /translation="MKAIGGTETLPAITDVGEQALVAWLAARGEPAYRAKQVRRHIAK
                     TPATGWSELTDLPKALREALAGSFRWMSVELVHEIESADGETRKALLRLHDGHHIESV
                     LMPHHGARNSVCFSTQAGCPMACAFCATGEMGLIRHLSTGEIVDQIRHWQRELVARGE
                     RVSHVVAMGMGEPLANLDAVAAAVRWLVDPELFGISPRRVTISTVGLVPQMDELAAMD
                     LPMNLAVSLHAPNDRIRHAIVPANKRWSVDDVLQASKRYVERTKRRVTFEYVLLSGVN
                     DAERDAVELAGRIAKLGHKGDYHVNLIPVNPGPGGFARPPVERMERFAEVLHERGIAA
                     TLRISKGQDIAAGCGQLKVPEGRAKVAT"
     gene            complement(1412..1621)
                     /gene="rpmB"
                     /locus_tag="VFV20_04950"
     CDS             complement(1412..1621)
                     /gene="rpmB"
                     /locus_tag="VFV20_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEU5287729.1"
                     /translation="MPRACAICGKTAAFGSNVSHSKVHTRRRFDANLHSAMIGGQKLL
                     VCSRCRRTQTKSARMAKKQEKAKAR"
     gene            1751..>2300
                     /locus_tag="VFV20_04955"
     CDS             1751..>2300
                     /locus_tag="VFV20_04955"
                     /inference="COORDINATES: protein motif:HMM:NF014754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEU5287730.1"
                     /translation="MNALNVYPVPDGDTGTNMLHTVRSALKYARAAESTLAAVSAAAA
                     HGALMGARGNSGVILSQIIRGLKEALASAETLGAAEVHRALALARTYAHEAVTVPAPG
                     TILTVTAAAADEEHSAGASGVRDVLAGVVRAADRAVARTKDENPTNKAAGVVDAGAKG
                     LFYLLDGALAAVEGRRVAATTVA"
BASE COUNT          339 a          787 c          861 g          313 t
ORIGIN      
        1 cgaggagctc gcgcggcgcg tgcgcggctg cgctccggac ccgggcgcat tcacgacata
       61 tcgcgggcaa cgcctcgtcg tcgcacgtgc gtcggtcacc ggtggcgcgc cgaaggagca
      121 cggcgtcgtc gaggtccgcg ccggcgtgcc gcacgtcgcg tcgggtgcgg gctggctgcg
      181 cctcgacgag gtccgacccg cggggaagcg cacgatgagc ggcgcggagt gggcgcgcgg
      241 cgtccgggag ctggacggtg cgcgcctgcc gagctgaccg cgtggaatcg ctcgcgacgt
      301 accctcgata catgaaggcg atcggtggca cggagaccct gcccgccatc acggacgtcg
      361 gcgaacaggc gctcgtcgcg tggctcgccg cgcgcgggga gccggcgtat cgagcgaagc
      421 aggtccgccg gcacatcgcg aagacgccgg cgacgggctg gagcgagctc acggatctgc
      481 cgaaggcgct gcgcgaagcg ctcgcgggct cgttccggtg gatgtcggtc gagctggtgc
      541 atgagatcga gagcgccgac ggcgagacgc gcaaggcgct gctgcgcctg cacgacgggc
      601 atcacatcga gtcggtcctc atgccgcatc acggcgcccg caactcggtg tgcttctcca
      661 cgcaggcggg ctgcccgatg gcctgcgcgt tctgcgcgac gggcgagatg ggactgatcc
      721 gccatctcag cacgggcgag atcgtcgacc agatccggca ctggcagcgg gagctcgtcg
      781 cacgcgggga gcgcgtctcg catgtcgtcg ccatggggat gggggagccg ctcgcgaacc
      841 tcgacgcggt cgccgccgcg gtgcgctggc tcgtcgatcc ggagctcttc ggcatcagtc
      901 cgcgccgcgt gaccatctcg acggtcgggc tcgtgccgca gatggacgag ctcgcggcga
      961 tggacctgcc gatgaacctc gcggtgtcgc tccacgcgcc gaacgaccgg atccgccacg
     1021 cgatcgtccc cgccaacaag cgctggagcg tggacgacgt gctgcaggcc tcgaagcgct
     1081 acgtggagag gacgaagcgg cgcgtgacct tcgagtacgt gctgctatca ggcgtgaacg
     1141 acgcggagcg cgacgcggtc gagctcgcgg gccggatcgc gaagctcgga cataagggcg
     1201 attaccacgt gaatctcatc ccggtgaatc ccggcccggg cggattcgcg cgcccgcccg
     1261 tcgaacgcat ggagcgcttc gcggaggtgc tgcacgagcg cggcatcgcg gcgacgctgc
     1321 gcatcagcaa ggggcaggac atcgccgcgg gttgtggtca gctgaaggtg ccggagggac
     1381 gggcgaaggt cgcgacgtaa aggcgagtgc cctagcgggc cttggccttc tcctgcttct
     1441 tcgccatgcg tgccgacttg gtctgggtgc gccggcagcg cgagcagacg agcagcttct
     1501 ggccgccgat catcgccgag tgcaggttcg cgtcgaagcg acggcgcgtg tgcaccttcg
     1561 agtggctcac gttggatccg aatgcggcgg tcttcccgca gatcgcgcag gcgcgtggca
     1621 tcggcggcac tcctcagggg aactgaagcc caatggtaca gtcgcgcaca tctcgacgca
     1681 atgcgacggc gccaccttac tccgcgtcct cgaggccgcc gccgaacgcc tggagcgcgc
     1741 ggtcgacgag gtgaacgcgc tcaacgtcta tccggtgccc gacggggaca ccggcacgaa
     1801 catgctccac accgtgcgct cggcgctgaa gtacgcgcgc gccgccgagt ccacgctcgc
     1861 ggcggtgagc gccgccgccg cgcacggcgc cctcatgggc gcgcgcggca acagcggcgt
     1921 gatcctctcg cagatcatcc gcggcctgaa ggaggcgctc gcctccgccg agacgctcgg
     1981 cgccgcggag gtgcaccggg cgctggcgct cgcgcgcacc tacgcgcatg aggcggtgac
     2041 cgtgcccgcg ccgggcacga tcctcacggt caccgccgcc gctgccgacg aggagcattc
     2101 ggccggtgcc tccggcgtgc gcgacgtgct cgcgggtgtg gtgcgcgccg cggaccgcgc
     2161 cgtggcgcgc accaaggacg agaacccgac gaacaaggcc gccggggtcg tcgacgcggg
     2221 tgcgaagggc ctcttctacc tgctcgacgg tgcgctcgcg gcggtcgagg ggcgcagggt
     2281 cgcagcgaca acggtcgccg
//
LOCUS       DASTZR010000223         2597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_613486, whole genome shotgun sequence.
ACCESSION   DASTZR010000223 DASTZR010000000
VERSION     DASTZR010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613486"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VFV20_04960"
     CDS             complement(<1..456)
                     /locus_tag="VFV20_04960"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5287731.1"
                     /translation="MIELSLFRTGAVDIARGAFAPWLDGPAREERTVAPVWSALLRTP
                     DGNILFDTGLHPVHVARPEATFGPQSGLRVVMEDGDAIVARLASLGVRPDDISIVVNS
                     HLHFDHAGNNGAFPNATFIVQADHLAFAKGRPNFPGVYWDIAELSYDPVS"
     gene            493..1158
                     /locus_tag="VFV20_04965"
     CDS             493..1158
                     /locus_tag="VFV20_04965"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF013398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HEU5287732.1"
                     /translation="MTELSHLHLPADDEEIRAPELVRHRELTDEQWDRFSAHMAEIFG
                     AFGLRLDTPGTRDTPRRFVRALFDATEGYEGDPKLLTAFPTECKGGAACHVDQIVEGP
                     IPFFALCEHHALPFYGNAYVGYVAHEQIIGISKLTRLVRVWAKRFTVQERMGDEIADT
                     LQSLIAAHAVAVHIEAHHTCTQMRGVRELHPKTRTAVWRGEYAKDAHLREEFFQLTRT
                     RAD"
     gene            1158..1979
                     /locus_tag="VFV20_04970"
     CDS             1158..1979
                     /locus_tag="VFV20_04970"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEU5287733.1"
                     /translation="MHDTGVLATLLDRQHGDELPLGAGLTKRYGGLLRFPARRPYVFA
                     NFVSTIDGVVSYDVPGIESAAEVSRGNDADRFVLALLRAVADAVVVGAGTLRKEASHV
                     WTPEHVYPHAKADFAALRASLGLRPRPLTVIVTASGELDLSLPVFRSEVVIATTAEAA
                     RGMSAPPGVRVVAVAERAPLPPRDIVALAAREAAGGRVLTEGGPSLLGSFLEHGVVDE
                     LFLTVAPHLAGRSDNVRRLALVEGVAFTPAAAPRGRLISVKSADDYLFTRFALTP"
     gene            2053..2559
                     /locus_tag="VFV20_04975"
     CDS             2053..2559
                     /locus_tag="VFV20_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287734.1"
                     /translation="MRMGRTASAVLLVVATSCGSATGTLGGATATPAPSPAATTRSAE
                     ITKPPQPSQVLPPPTYARWAIDQSADPSKRLFVLFYDAPALGFRLLDAAGEVIVRVPI
                     AGSGIFGPDTCVASAHRANEITTWAGVDEPTYRRILANIATYRLEADAVGGPTVTIPL
                     SDSGCRKA"
BASE COUNT          379 a          940 c          889 g          389 t
ORIGIN      
        1 gctcaccgga tcgtagctga gctccgcgat gtcccagtag acgcccggaa agttcggcct
       61 tcccttcgcg aacgcgaggt ggtcggcctg cacgatgaag gtcgcgttcg ggaaggcgcc
      121 gttgttcccg gcatggtcga agtgcagatg gctgttcacg acgatgctga tgtcgtcggg
      181 gcgcacaccc agcgatgcca ggcgtgccac gatcgcgtcg ccgtcctcca tgacgacgcg
      241 cagaccgctc tgcggtccga acgtcgcctc gggccgtgcc acgtgcaccg gatggagtcc
      301 ggtgtcgaac aggatgttgc cgtccggtgt ccgcaggaga gcggaccaaa ctggagcgac
      361 ggtacgctcc tcacgtgcgg gtccgtcgag ccacggtgcg aaggcaccgc gcgcgatgtc
      421 gacggcaccg gtacggaaca gactcagctc gatcacgtgt tctcgacatg atgaccgtta
      481 actggaggcc ccatgactga gctctcgcac ctgcacctcc cggcggacga cgaggagatc
      541 cgcgcgcccg agctcgtccg gcaccgagag ctcaccgacg agcagtggga ccgcttctcg
      601 gcgcacatgg ccgagatctt cggcgcgttc gggctccgcc tcgacacgcc cggcacccgc
      661 gacacgccca ggcgcttcgt ccgcgcgctc ttcgacgcca ccgaggggta tgagggcgat
      721 cccaagctgc tgaccgcctt ccccacggag tgcaagggcg gcgccgcgtg ccacgtcgac
      781 cagatcgtcg agggcccgat cccgttcttc gcgctctgcg agcaccacgc gctcccgttc
      841 tacgggaacg cctacgtcgg ctacgtcgcg cacgagcaga tcatcggcat cagcaagctg
      901 acgaggctcg tccgcgtctg ggccaagcgc ttcaccgtgc aggagcgcat gggcgacgag
      961 atcgccgaca cgcttcaatc gctcatcgcc gcgcacgccg tcgccgtgca catcgaggcg
     1021 catcacacct gcacgcagat gcgcggtgtc cgcgagctgc atccgaagac gcggaccgcg
     1081 gtctggcgcg gcgagtacgc gaaggacgcg cacctgcgcg aagagttctt ccagctgacc
     1141 cgtacccgcg cggactagtg cacgacaccg gcgtgctcgc gacgctgctg gaccgtcagc
     1201 atggcgacga gctcccgctc ggcgccgggc tgacgaagcg ctacggcggg ctgctgcgat
     1261 tcccggcgcg acggccgtac gtgttcgcga acttcgtgag caccatcgac ggcgtggtct
     1321 cgtacgacgt gcccgggatc gaaagcgcgg cggaggtgag ccgcgggaac gacgccgatc
     1381 gattcgtgct cgccctgctg cgtgcggtcg cggacgcggt ggtcgtcggc gcgggcaccc
     1441 tgcgcaagga ggcctcgcac gtctggacgc cggagcacgt gtacccgcac gcgaaggccg
     1501 actttgccgc gctccgcgca tcgctcgggc tgcggccgcg cccgctcacc gtgatcgtga
     1561 ccgcgtccgg cgagctcgat ctctcgctgc ccgtgttccg ctcggaggtg gtgatcgcga
     1621 ccaccgccga ggccgcgcgc ggcatgtcgg cgccaccggg cgttcgtgtc gtcgccgtgg
     1681 ccgagcgtgc gccgctcccg ccgcgcgaca tcgtcgccct cgccgcgcgc gaggccgccg
     1741 gaggacgcgt gctcaccgag ggcgggccga gcctcctggg aagcttcctc gagcacggtg
     1801 tcgtcgacga gctcttcctc accgtggcac cacatctcgc gggtcgcagt gacaacgtgc
     1861 gccggctcgc gctcgtcgag ggcgtcgcat tcacgcccgc cgcggcgccg cgtggccggc
     1921 tgatctcggt caagtcggcg gacgattacc tgttcacgcg cttcgcgctc acgccctagt
     1981 catcccgcga cgtacgagtt aacgcgcata cctcggacgc gggcgcgatc cataggtgcg
     2041 acgctcgtgc cgatgcggat gggtcgcacg gcctccgccg tcctgctcgt cgtcgcgacg
     2101 tcgtgtggaa gcgcgaccgg aacgctcggg ggcgcgaccg ccacgccggc gccgtcgccg
     2161 gccgccacga cgcgcagcgc cgagatcacg aagccgccgc agccgtccca ggtgctgccg
     2221 ccgccgacgt acgcgcgctg ggcgatcgac cagagcgccg acccatcgaa gcgcctcttc
     2281 gtcctcttct acgacgctcc ggccctgggc ttccgtctcc tcgacgcggc cggcgaggtg
     2341 atcgtgcgcg tgccgatcgc cggatcgggc atcttcggcc ccgatacatg cgtcgcgagc
     2401 gcccaccgcg cgaacgagat cacgacgtgg gcgggcgtgg acgagccgac gtaccggcgc
     2461 atcctcgcga atatcgcgac ctaccggctc gaggccgacg cggtgggcgg accgaccgtg
     2521 acgatcccgc tcagcgacag cgggtgccgg aaggcgtagc tgcgctctcc gagtcgcgtc
     2581 gtcgcgtccg cgtggcg
//
LOCUS       DASTZR010000224         4805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_349691, whole genome shotgun sequence.
ACCESSION   DASTZR010000224 DASTZR010000000
VERSION     DASTZR010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349691"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VFV20_04980"
     CDS             <1..696
                     /locus_tag="VFV20_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU5287735.1"
                     /translation="LGTSAVVVMPRHVSPTIVGVCRAYGAEVILEGDVFDDAEVLAHR
                     IEREQGRTFIHPYADRLIVAGQGTIGLELLEELPDLDQVVVAVGGGGLSAGIALAVKS
                     ERPRVRVIGVEPAGSDEVGRSVRAGAPVVLERPASVADKLVARTTMPMAVELGRRYMD
                     DYVTVSEEALEAATYELLDRLSLLIEPSGAAPLAAVREGKLALRGLRTVLIASGGNIA
                     PAVLARILRERTT"
     gene            693..1100
                     /locus_tag="VFV20_04985"
     CDS             693..1100
                     /locus_tag="VFV20_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEU5287736.1"
                     /translation="MTVHHEELTVASGKKLSTTDITARVRAAVTRSKVRDGICVIATM
                     HTTAGIFVNENADPDVQRDLIAHLGKLVPADDEFRHAEGNADAHIKAVLTGNDVALPI
                     RDGELVLGTWQGIYFADYDGPRERHATVTVIGD"
     gene            1165..2565
                     /locus_tag="VFV20_04990"
     CDS             1165..2565
                     /locus_tag="VFV20_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEU5287737.1"
                     /translation="MTRSPAVSARDAERALRTALSRGGDFAELFWERHETLVLTLDDG
                     RIEDAITGVDQGAGIRVTSGERTVYANGNVTEADDVLALAGRAAASVADGELHGAAHL
                     LPDELPRTHPVAIDPRSVPVERKVALLRTADRVARAFDERVIQVTASYSESVQEVLVA
                     NSDGTSRTDTRVRLNLALNVVARRGDVLESGFEALRGTRGFEMLDDDVVADTATIAAR
                     RAVLNVGADPAPAGTYVVVLSSEAGGTLIHESVGHGLEADLNQKGLSVYSGRIGQKVA
                     SELITVVDDGRDPGQRGTAAMDDEGTPTQRTVLIEKGVLKTYLSDRKHAEKLGIARSG
                     NARRESFRHLPICRMTNTMIERGDSDPDAILASVKDGIFVKKMGGGQVDVVSGNFAFE
                     ISECYRIRDGKLAEPLRGATLVGQGPKLMSEIDMVGWDLGYSTGTCGKDGQGAPVADA
                     QPTLRIPSVVVGGKLK"
     gene            2588..3940
                     /locus_tag="VFV20_04995"
     CDS             2588..3940
                     /locus_tag="VFV20_04995"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEU5287738.1"
                     /translation="MTQRLADDEVVERALSALRAARVATAEVFLRDAQSGSVETKDGA
                     IDTVITRGERGLGVRVLEEERMGFAHSSDLSVPGIEACVDQARRMAKITEPDADLAIA
                     SAPIASVDLGIAHPGLEEHALAERGRVALEVERAARGSDARITHFRKTSYSDAAVSTI
                     IATTTGLRASYSETYCSVATSPVATQDGERQIGYHGEAARRREDIDPHRVGAQAARRA
                     TQKLGARPFPTQKLPVVFDPWMSMSLLGAIAPLFSADNVLKGRSLFAGKTGQRVASEH
                     VTIVDDGRRAGGLRSAPFDGEGIATTTRTLVERGALRGYLTNMKTARKLGGSSKGNAR
                     RGSYASPSRIGPSNFYVEAGVTDPVALLRGLDRALAITSLLNLHTIDPISGEFSLGAT
                     GTYLERGAPVQAVQGVTIAGNLTHLLTSISAVGNDLTFGPGGVGSPTLVVAELSIGGT
                     "
     gene            3940..>4805
                     /locus_tag="VFV20_05000"
     CDS             3940..>4805
                     /locus_tag="VFV20_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287739.1"
                     /translation="MAWHALLHGDPVPWLLERSDPAVRAMTLRQLLGRGPRDAELREA
                     QQRAMSSPPISTLLKRQHADGGWNGANMYGPKYQSTHWSTLLLIEYGADPSDARIRRA
                     ARHVLEDLQDPEAHGVGWVFKDDHGISCFVGNVVRYVATAGYGADARVEPLVERLVRD
                     SKKYDAACAINGDDPCAWGYARLIWGLAALPEAARTREVERTLRRGVEFLLSYKLERG
                     RYPTATEPSYLWRQLSFPLFYQADVLFVLRAIDAAGEIDDPRAQPAIAWLLARQDPRG
                     RWAGRAPYADRMP"
BASE COUNT          739 a         1670 c         1720 g          676 t
ORIGIN      
        1 ctgggcacgt ccgcggtcgt cgtcatgccg cgccacgtct cgccgaccat cgtcggcgtc
       61 tgccgcgcct atggcgccga ggtgatcctc gagggcgacg tcttcgacga cgccgaggtc
      121 ctggcgcacc gcatcgaacg cgagcagggt cggacgttca tccatccgta cgcggatcgg
      181 ctcatcgtgg ccggccaggg cacgatcggc ctcgaattgc tcgaggaatt gcccgacctc
      241 gaccaggtcg tcgtggccgt cggcggcggc ggtctgtccg cggggatcgc gctcgcggtg
      301 aagagcgagc ggccgcgtgt gcgtgtgatc ggcgtcgaac ccgcgggctc ggacgaggtc
      361 ggtcgctcgg tccgtgcggg cgcgccggtc gtcctcgagc ggccggcgag cgtcgccgac
      421 aagctcgttg cccgcacgac catgccgatg gccgtggaac tgggccgccg ctacatggac
      481 gactacgtga ccgtgagcga ggaggcgctc gaggccgcca cctacgagct actggaccgc
      541 ctgtcgctcc tcatcgagcc ctccggcgcc gcgccgctgg cggccgtgcg cgagggcaag
      601 ctcgcccttc gcggtctccg gaccgtgctc atcgcgagcg gcggcaatat cgccccggcc
      661 gtcctcgcgc ggatcctgcg ggagcggacc acgtgaccgt tcaccacgaa gagctcacgg
      721 tcgcgagcgg gaagaagctc tcgacgacgg acatcaccgc gcgcgtgcgc gcggccgtca
      781 cacggtcgaa ggtgcgggac gggatctgcg tgatcgcgac gatgcacacg accgccggca
      841 tcttcgtcaa cgagaacgcc gatcccgacg tgcagcggga cctcatcgcg cacctcggga
      901 agctcgttcc cgccgacgac gagttccgtc acgcggaggg gaacgcggac gcgcacatca
      961 aagcggtgct cacgggcaac gacgtcgcgc tgcccatacg cgatggtgag ctcgtactgg
     1021 gtacgtggca ggggatttac ttcgcggatt acgacggacc gcgcgaacgt cacgccaccg
     1081 ttactgtcat cggcgactag cagtcgtacc atttgcccgc cgccaccggt ggcaagggga
     1141 gagggcatca gcagcacgct cgcgatgacg cgttcacctg ccgtgtccgc gcgcgacgca
     1201 gagcgcgcgc tgcggacggc gctttcgcgt ggtggcgact tcgccgagct gttctgggag
     1261 cgacacgaga cgctggtgct cacgctggac gacggccgca tcgaggacgc gatcaccggt
     1321 gtcgaccagg gtgcgggcat ccgcgtcacc agtggcgagc gcaccgtcta cgcgaacggc
     1381 aacgtcaccg aggccgacga tgtcctcgcg ctcgcgggac gcgcggctgc aagcgtggcg
     1441 gacggcgagc tgcacggcgc tgcgcatctg cttccggacg agcttccgcg cacccatccg
     1501 gtcgcgatcg atccgcggag cgtgccggtg gagcggaagg tggcgctcct gcgcaccgcg
     1561 gaccgggtcg cgcgcgcgtt cgacgagcgg gtcatccagg tcacggcctc ctactcggag
     1621 agcgtccagg aggtcctggt ggccaacagc gacgggacct cgcgcacgga cacgcgggtc
     1681 cggctcaacc tcgccctgaa cgtggtggcc aggcggggag acgtcctcga gtcgggcttc
     1741 gaggcccttc gcggcacgcg cggcttcgag atgctcgacg acgacgtcgt cgccgacacc
     1801 gctaccatcg ccgcgcgccg cgccgtcctg aacgtcggtg ctgatcccgc gccggcgggg
     1861 acgtacgtcg tcgtgctctc gagcgaggcc ggtgggacgc tcatccacga gtcggtcgga
     1921 cacgggctcg aggccgacct caaccagaag ggcctctccg tgtactccgg gcggatcggt
     1981 cagaaggtcg ccagcgagct catcaccgtc gtggacgatg gccgtgatcc cggacagcgc
     2041 ggtacggccg cgatggacga cgaggggacg ccgacgcagc ggacggtgct gatcgagaag
     2101 ggcgtgctga agacatacct ctcggaccgg aagcacgccg agaagctggg catcgcgcgc
     2161 agcggcaacg cgcggcgcga gtccttccgg cacctgccga tctgccggat gacgaacacc
     2221 atgatcgagc gcggcgactc cgatcccgac gcgatcctcg cgtcggtgaa ggacggcatc
     2281 ttcgtgaaga agatgggtgg cggccaggtc gacgtcgtct cgggcaactt cgcgttcgag
     2341 atcagcgagt gctaccgcat ccgcgacggc aagctcgccg agccgctgcg cggcgcgacc
     2401 ctcgtcggcc agggtccgaa gctgatgagc gagatcgaca tggtcggctg ggacctcggg
     2461 tactccaccg ggacgtgcgg caaggacggc cagggcgcgc cggtcgccga cgcgcaaccc
     2521 acgctccgca tcccctcggt cgtcgtgggc gggaagctga agtgagtgac gacgagggct
     2581 tgggcacgtg actcagcggc tcgcagatga cgaggtcgtc gaacgggcgc tctcggcgct
     2641 gcgcgcggcg cgcgtcgcca cggccgaggt gttcctgcgc gacgcgcagt cgggctcggt
     2701 cgagacgaag gacggggcga tcgataccgt catcacgcgt ggcgagcgcg gccttggtgt
     2761 gcgcgtcctc gaggaagagc gcatgggctt cgcgcactcc tccgacctga gcgttccggg
     2821 tatcgaggcc tgcgtcgacc aggcacgccg catggcgaag atcacggagc cagacgcgga
     2881 cctcgcgatc gcgagcgcac cgatcgcgtc tgtggacctc ggtatcgcgc atcccggtct
     2941 cgaggaacac gcgctcgccg agcggggcag ggtcgcgctc gaggtcgagc gggcggcgcg
     3001 cgggtcggac gcgcgcatca cgcacttccg caagacctcg tacagcgacg ccgcggtcag
     3061 cacgatcatc gcgacgacca cgggccttcg cgcctcatac agcgagacct attgcagcgt
     3121 cgcgaccagt cccgtcgcga cgcaggacgg cgagcggcag atcggctacc acggcgaggc
     3181 ggcccgcagg cgcgaggaca tcgatccgca tcgggtcggc gcgcaggctg cgcggcgcgc
     3241 cacccagaag ctcggcgcgc gaccgttccc cacgcagaag ctccccgtcg tcttcgaccc
     3301 ctggatgagc atgagcctgc tcggcgcgat cgcgccgctc ttctccgcgg acaacgtgct
     3361 gaagggacgc agcctcttcg cgggcaagac cggccagcgc gtcgcgagcg agcacgtcac
     3421 gatcgtggac gacgggcgcc gcgcgggcgg gttgcgcagc gcgccgttcg acggggaggg
     3481 catcgccacg accacccgca cgctcgtcga gcgcggggcg ctgcgcggct atctcacgaa
     3541 catgaagacc gcccgcaagc ttggcggatc gtcgaagggg aacgcgcgcc gcggatcgta
     3601 tgcgtccccg agccgcatcg gtccgtccaa cttctatgtt gaagccggcg tgacggaccc
     3661 cgtggcgctc ctgcgcggcc tcgatcgagc gctcgcgatc acgagcctcc tcaacctgca
     3721 cacgatcgat ccgatctcgg gcgagttctc gctcggcgcg actgggacgt acctggagcg
     3781 gggcgcgccc gtccaggcgg tccagggtgt gacgatcgcg gggaatctga cgcatctgct
     3841 gacgtccatc tccgccgtgg ggaacgacct caccttcgga cccggtggcg tcggcagccc
     3901 gacgctcgtc gtcgccgagc tgtcgatcgg aggcacgtga tggcctggca cgcgctcctc
     3961 cacggcgatc cggtgccgtg gctcctcgag cgatcggatc ccgccgttcg cgccatgacg
     4021 ctgcggcagc tgctcggtcg cggtccgcgc gatgccgagc tgcgcgaggc gcagcagcgc
     4081 gcgatgagct cgccgccgat ctcgacactg ctgaagcgac agcacgccga cggcggctgg
     4141 aacggcgcga acatgtacgg cccgaagtac cagagcacgc actggtcgac gctcctgctc
     4201 atcgagtacg gagcggaccc gagcgacgcg cgcatccgcc gggccgcccg acacgtcctc
     4261 gaagacctgc aggatcccga ggcgcacggc gtgggatggg tgttcaagga cgaccacggc
     4321 atctcgtgct tcgtcggcaa cgtcgtccgc tacgtcgcga cggccggata cggcgcggac
     4381 gcccgcgtcg agccgctcgt ggagcgtctc gtacgcgatt cgaagaagta cgacgcggcg
     4441 tgcgcgatca acggtgacga cccgtgcgcg tggggctacg cccggctcat ctggggactc
     4501 gccgcgctcc ccgaggcggc gcgcacccgc gaggtcgagc ggacgctgcg acgcggcgtc
     4561 gagttcctgc tgtcgtacaa gctcgagcgg ggccgctatc ccaccgcgac ggagccgagc
     4621 tacctgtggc ggcagctgtc gttcccgctc ttctaccagg ccgacgtgct cttcgtgctc
     4681 cgcgcgatcg acgcggcggg cgagatcgac gacccgcgcg cgcagccggc catcgcgtgg
     4741 ctcctcgcgc ggcaggaccc gcgcgggcgg tgggcgggtc gcgcacccta tgcggaccgc
     4801 atgcc
//
LOCUS       DASTZR010000225         3377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_975254, whole genome shotgun sequence.
ACCESSION   DASTZR010000225 DASTZR010000000
VERSION     DASTZR010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975254"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(318..1355)
                     /locus_tag="VFV20_05005"
     CDS             complement(318..1355)
                     /locus_tag="VFV20_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006076636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HEU5287740.1"
                     /translation="MSFEHGKGRSWDPPRDWRRVSVIDSHAAGEPLRVVLDGIDRVPG
                     ATIVAKRAWAREHLDAVRRGLMFEPRGHADMYGAIVTEPVTSDGDLGVLFMHNEGWST
                     MCGHGIIALTTVALETGMLSPRDVVRIDAPAGRIVARPKRQGGRVASVVFENVPSFVL
                     ARDETVDVAGLGRIRYDIAFGGAFYAYVDVSQVGLEMTARDNRGLIDAGWRIKKAVMA
                     QRPIPHPFEPELSFLYGTIFTGPALDPGGDSRNVCVFADGEVDRSPTGTGVSGRVALE
                     HARGRLPAGRPFVVESIIGTRFTGRVARETTFGPHAAVIPEVEGSAWLSGRGELWYDP
                     RDPLSEGFILR"
     gene            complement(1397..1627)
                     /locus_tag="VFV20_05010"
     CDS             complement(1397..1627)
                     /locus_tag="VFV20_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287741.1"
                     /translation="MPQDAWSPKRERQYKHIKQNEKERGRSEGTAERIAAATVTRTGT
                     AKGETKTPQPKTKRRSAQRAAKAASSRNAKKR"
     gene            1685..2893
                     /locus_tag="VFV20_05015"
     CDS             1685..2893
                     /locus_tag="VFV20_05015"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5287742.1"
                     /translation="MDVAERPSYRALFAISGFGRLVASMMLARVAQQMVALVLVLLAL
                     QRYGSAEVAGIVTFAAVAPGLVAAPIAGALLDRHGRTRLVVLDYALNALALGLIAFLA
                     SADALPVPLLVVIVTVSSITSPMSNTGVRTLFPLIVPMPLWERANAVDSNGYVVASIF
                     GPAMAGALVAAVGPASALGIIASIFVAAAIAATGLRDPGARTAGTRLLSDAWQGLVYV
                     VRHPTLRALALSVSTSNVSQGLFFIALPVLVLQRFHAGAEFVGLLFALMGISGFVSVL
                     LVGRVSSRGRERLFLAGSMLGMAAAFAVVLLGAASPLVVAIGMLGVGIANGPFDVALF
                     TVRQRRTDPAWMGRAFAVSMALNFAGFPLGSALGGAIVPASIEAALVIAVIVNVVAAA
                     MTVLWLPRED"
     gene            complement(2904..>3377)
                     /locus_tag="VFV20_05020"
     CDS             complement(2904..>3377)
                     /locus_tag="VFV20_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287743.1"
                     /translation="GRLEAVLATGASRARVVVERLAALLVVGGVVAAVSSAVVYYGAR
                     ALDIAVPGDRMVIATADTLPVMFAFGGIGAALVGWRPRAAVVLLGAVAVTSYFLQQFY
                     AIFEWPEWVGHLSIYQLYGTPLTSDDWPGIIALVAIGVAGMAIAIVSMQKRDVGT"
BASE COUNT          425 a         1242 c         1188 g          522 t
ORIGIN      
        1 gaccgccgcg ccatgacgcc cttcttcgtc tagctccgcg cgccgccgct acggccggcg
       61 gcggacacac cgcgccgcgc gagctagctg aagtctcctt acacgtcggt cacgtccgcg
      121 tggcggcggg tggcacgcgc agggcgttgt tctcaccgaa gcgaagtccg gacccggacg
      181 cccggacgcg agcggtccat cacgcgcacc ggccgacggg cgggccgggg agccagactt
      241 cgcgagggga gaacgacgcc cggagcggga caggacccgc cgccacacgg atgcgactgg
      301 agtctgggag accgcagcta gcgaagaatg aaaccttcgg agagcggatc gcgcgggtcg
      361 taccagagct cgccgcggcc cgacagccac gcgctccctt cgacctccgg gatcacggcg
      421 gcgtgcgggc cgaaggtggt ctcgcgcgcg acgcgaccgg tgaagcgcgt gccgatgatg
      481 ctctcgacga cgaacggccg gcccgcgggg agccgcccgc gtgcgtgctc gagcgcgacg
      541 cggccggaca cgccggtacc ggtcggcgaa cgatccacct cgccgtccgc gaagacgcag
      601 acgttgcgcg agtcgccgcc gggatcgagc gcgggtccgg tgaagatcgt tccatagagg
      661 aacgacagct cgggctcgaa gggatggggg atcggccgct gtgccatgac cgccttcttg
      721 atccgccagc cggcgtcgat gagcccgcga ttgtccctgg ccgtcatctc gaggccgacc
      781 tgcgacacgt ccacgtacgc gtagaaggcg ccgccgaacg cgatgtcata gcggatccgc
      841 ccgagtccgg ccacgtccac cgtctcgtcc cgcgcgagca cgaatgacgg gacgttctcg
      901 aagacgacgc tcgcgacgcg gccgccctgg cgcttcggtc gcgcgacgat gcgtcccgcg
      961 ggagcgtcga tgcgcaccac gtcgcgcggc gacaacatgc ccgtttccag cgcgaccgtg
     1021 gtgagcgcga tgatcccgtg cccgcacatc gtgctccagc cctcgttgtg catgaacagc
     1081 accccgaggt cgccgtcgga agtcaccggt tcggtgacga tcgcgccgta catgtcggcg
     1141 tggccgcggg gctcgaacat gaggccgcgg cgcacggcat cgaggtgctc tcgcgcccac
     1201 gcccgcttcg cgacgatggt cgcaccgggg accctgtcga tcccgtcgag cacgacgcgc
     1261 agcggctcgc cggcggcgtg cgaatcgatg acgctgacgc gacgccagtc gcgcggcgga
     1321 tcccagctcc ggcccttgcc gtgctcgaag gacatcggcg cgagcctacc gcgctacgcc
     1381 gagcgtcgcc tcggcgtcag cgcttcttcg cgttgcggct gctcgccgcc ttcgccgcgc
     1441 gctgcgcgct gcgccgcttc gtcttcggct gcggcgtctt cgtctcgccc ttcgcggtgc
     1501 ccgtgcgagt caccgtcgcg gccgcgatgc gctccgccgt tccctccgac cggccgcgct
     1561 ccttctcgtt ctgcttgatg tgcttgtact ggcgctcgcg cttcgggctc cacgcgtcct
     1621 gtggcatgcg gcacctccgc tcgccgccat gcatgtcccg tgcgcgctag ccttgtgccc
     1681 tcccatggac gtcgccgaac ggccgagcta ccgcgcgctc ttcgccatct ccggcttcgg
     1741 gcggctcgtc gccagcatga tgctggcgcg cgtcgcgcag cagatggtcg cgctcgttct
     1801 cgtgctgctc gcgctgcagc ggtacgggtc tgcggaggtc gcgggcatcg tcacgttcgc
     1861 cgccgtggcg ccgggcctcg tcgccgcgcc gatcgccggc gcgctgctcg accggcacgg
     1921 tcgcacgcgc ctcgtcgtcc tcgactacgc cctgaacgcg ctcgcgctcg ggctcatcgc
     1981 gttcctcgcg agcgcggacg cgctgcccgt cccgctcctc gtcgtcatcg tcacggtgtc
     2041 atcgatcacg agcccgatgt cgaacaccgg cgtgcgcacg ctcttcccgc tcatcgtccc
     2101 gatgcccctc tgggagcgcg cgaacgccgt cgacagcaac gggtacgtcg tcgccagcat
     2161 cttcggtccg gcgatggcgg gcgcgctcgt tgccgcggtc ggcccggcct ccgcgctcgg
     2221 gatcatcgcg tcgatcttcg tcgccgcggc catagccgcg acgggtctgc gcgaccccgg
     2281 ggcgcggacc gcggggacgc gcctgctcag cgacgcgtgg cagggcctgg tctacgtcgt
     2341 gcggcacccg acgctgcgcg cgctcgcgct gtcggtctcg acgagcaacg tgtcgcaggg
     2401 tctgttcttc atcgcgctgc cggtgctcgt gctgcagcgc ttccacgcgg gtgccgagtt
     2461 cgtcggcctc ttgttcgcgc tcatgggcat ctcgggattc gtgtcggtgc tgcttgttgg
     2521 gcgcgtgtcg tcgcgcgggc gcgagcggct cttcctcgcc ggctccatgc tcggcatggc
     2581 cgccgccttc gcggtcgtgc tgctcggcgc cgcgagcccg ctggtcgtcg cgatcgggat
     2641 gctcggcgtc gggatcgcga acggcccgtt cgacgtggcc ctgttcaccg tccggcagcg
     2701 gcgcaccgat ccggcgtgga tgggacgagc gttcgccgtc tcgatggcgc tgaacttcgc
     2761 cgggttcccg ctcggatcgg cgctcggcgg tgcgatcgtc cccgcgtcca tcgaggccgc
     2821 gctcgtcatc gcggtcatcg tgaacgtcgt cgccgcggcg atgaccgtcc tctggctgcc
     2881 gcgcgaagac tagcctcgcg cccttaggtg ccgacgtcgc gcttctgcat cgacacgatc
     2941 gcgatggcca tgcccgccac gccgatcgcg acgagcgcga tgatgcccgg ccagtcgtcg
     3001 gacgtgagcg gcgtgccgta gagctgatag atcgacaggt ggccgaccca ctcgggccac
     3061 tcgaagatcg cgtagaactg ctggaggaag tacgaggtga ccgcgaccgc gccgagcagg
     3121 acgaccgcgg cgcgcgggcg ccagccgacg agcgcggcgc cgatgccgcc gaaggcgaac
     3181 atgacgggga gcgtgtcggc cgtcgcgatg accatgcgat cccccggcac ggcgatgtcg
     3241 agcgcgcgcg cgccgtagta cacgacggcg gagctcacgg cggcgacgac gccgcccacg
     3301 acgaggagcg cggcgaggcg ctcgacgacg acgcgcgcgc gcgacgcgcc ggtggccagc
     3361 acggcctcga ggcggcc
//
LOCUS       DASTZR010000226        13139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1031353, whole genome shotgun sequence.
ACCESSION   DASTZR010000226 DASTZR010000000
VERSION     DASTZR010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 13139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13139
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031353"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1380
                     /locus_tag="VFV20_05025"
     CDS             172..1380
                     /locus_tag="VFV20_05025"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5287744.1"
                     /translation="MWRDLEFTKLWSGSLLSAAGTAITGLAMPLIAVGLGATPAQMGV
                     LGASGTAAFIVFGLAAGVIVDRAPRRSVLMLTSAGSAVVVATVPLAHLLGLLRIEQLY
                     AVEFVAGSLALVDQTAFQAILPRLVGRERLLESITLVRSSDSVTAVAGPSIAGVLVQV
                     LGAPVAILVDAASYLLQTVLTFLVRVTEPAAPERAPGRRVWHDVVEGLRYVLAEPSLR
                     FLALGGATHNVCSNGAIVALYVLYMSQRLSLSPVEIGLVFAAGGPSALLGSVLAGRWG
                     RRFGMRGALSQAQVLTGIARGFVPLASFVPSPLLALVAGELVLGIARSVFNVNQLSMR
                     MTLTPDHLQGRMTASIRFLMWSVVPVGALAGGFAAERFGLVPTLVVAALGTGLSALWF
                     LFIPPEERAV"
     gene            complement(1450..2769)
                     /locus_tag="VFV20_05030"
     CDS             complement(1450..2769)
                     /locus_tag="VFV20_05030"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5287745.1"
                     /translation="MRKLSVLAAIAMLIAACGGGSPTSPTPAATTEADLEITGPVEVT
                     LWHALTSDPQKGALEAAVKKFNDTNGKGITVKAIVQGNYTVLGQKVLGAIQAGALPEM
                     AHAYESNVADYTKADVVIDLDPFVNSKKNGLDQASKDDIYKPYFDTNRFAQYGNKLLS
                     FPFTKSLAVMYANDDALKAAGVATVPKTWDEWEQAVMKTTKKDASGKTTQYGYAGTSD
                     ASYFNAMVLSRGGKLMSDDGKTVAWDGKEGLATLQMLKRLYDGKYAYTPTGFDWQNDF
                     AQGKLAFTFSSTSSRPFIAAAMKTPVNWSVSVPPGGKTVMFGANIAVFKSTPQKQLAS
                     WLFIKWFSEQEQTADWATKSFYMPVRKAAANNEVLKKSWTTTDPQGKQAYDQIQNSIP
                     EPNVRGQQEIRNVIQDMYVSVVTGKATPEQAIKDAGVKANQILKDSQ"
     gene            complement(2847..3731)
                     /locus_tag="VFV20_05035"
     CDS             complement(2847..3731)
                     /locus_tag="VFV20_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU5287746.1"
                     /translation="MNVPTVIPRMSVPAVRRPQVPAVASSLTKHLVLIVAGGLVAYPF
                     YFMVSSSLKPFFEATLVPPTLWPHEVRLENYQEVWNKAPWARYFANTLLVAGSNTLGE
                     LVTSVLAAYAFAWMNFKGRAALFALLLATYMMPGEATLIPNFVIVSKRYLNLYDTYWV
                     QILPFLATAFSIFLLRQQFLSVPRELYDAAIIDGAGHLRFLWSVVLPVSVPALVTVSI
                     IAFYGSWNAFQWPLIVTSTPDIRPIQVGLNAFRAEAGSSYHLLMAAASFVVAPIVLLY
                     LVGQRYLVQGVARTGIRG"
     gene            complement(3728..4720)
                     /locus_tag="VFV20_05040"
     CDS             complement(3728..4720)
                     /locus_tag="VFV20_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU5287747.1"
                     /translation="MSGGAIGTGQRAASRRRRFIVETLQAYLFLLPAFAVLAVFKIWP
                     AFFAIYISFFKWDIIQGAFRGIDNYTDILFGSRAESFWRSVSTTFTYAAITIPLEIIF
                     ALVIAYVLFQRIRGRAIYRTIFYLPYITATVAAAAVFEWLYHPQYGAFNQVLGLVGIG
                     PLRFLQEPDGIFEMIGSTVGLTVPQWAAGPSLALVSVALFSIWHFVGFQTVVFLAGLG
                     NIPEQYYEAARLDGASQRQLFLKITLPLLTPQIFFVFTIASIGVLRAFNEIYVLTNGG
                     PLDTTRTVTMNVFRTFFQQGQIGLGSAMGVLLTLIILAFTLIQFRVLERRVQYG"
     gene            4741..5091
                     /locus_tag="VFV20_05045"
     CDS             4741..5091
                     /locus_tag="VFV20_05045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEU5287748.1"
                     /translation="MPIYDYRCEHCGHAFSALQSYNDDALATCPSCGKQPRRLIAPPA
                     IVFKGSGWYKTDSRAKPAAETASPAAKDGAKDGAKDGAKSDTKSDAKPDTKSDTKSEP
                     KPSAGSSGRSGAAS"
     gene            5118..5909
                     /locus_tag="VFV20_05050"
     CDS             5118..5909
                     /locus_tag="VFV20_05050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU5287749.1"
                     /translation="MRAAGASFLGDESAFLAGGVAYQVFFALIPMLALVVGVLAFVYG
                     PDRGEREFAQMLRQVYPSATAQEVRIARELVDGRAISFGFGLIGTVLSAGAVFGSIDT
                     ALALILGAGRRRGLVRGRLDGMAFIGAIALLAVLSFALSYGAQAAQGAFAAAGLARTG
                     RLLIALGSSLLGLLAGWLFFYVIYRYVPRVPVAPRVARTAALVSAVLWEVAKVAFGFF
                     TGGLGAFSAYGPIAFAAGLLTWIYLTAVIILFGAQFMKVRMRGAG"
     gene            5909..6655
                     /locus_tag="VFV20_05055"
     CDS             5909..6655
                     /locus_tag="VFV20_05055"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HEU5287750.1"
                     /translation="MERVEAFVSRVIEGWSARLFGAKLQPVQIAKRLIRAMEAHQTIS
                     LSKTFVPNSYVVSLGPTDFAQFDQYRRSLERDLAETLLGAARERNYTLLAYPTVEIER
                     DDDMTPGDIRVSCALVDASGEEVEADAKTVGAVESGHTMVLDREALLRERPRAPRASL
                     ERRDGPAVPLGPDPVLIGRDPQNDVVLDDRRVSRKHAEIRLRLGRYTLYDLQSTNGTY
                     VNGRRVAEKVLEDGDHVSVGGLEIVFHSAD"
     gene            6747..7175
                     /locus_tag="VFV20_05060"
     CDS             6747..7175
                     /locus_tag="VFV20_05060"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEU5287751.1"
                     /translation="MELTFGALLWAVRIAFLLLIYIFLIRAFGVLQRALAAERVEGGA
                     VGTLVVERSPARTPRVGERLPLRASNAIGRDAGNDIALPDEAASARHATLALEDGDWW
                     IEDLGSTNGTLVNGTRITKRERLRPGDEIAIGRVALRLER"
     gene            7186..8478
                     /locus_tag="VFV20_05065"
     CDS             7186..8478
                     /locus_tag="VFV20_05065"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU5287752.1"
                     /translation="MTALLFRRSSELGLLVWVAAIAAAGSVAVAAAETSAFDRAQLAI
                     PVVFVALAVALHVFLAARGIRGDQLLLPLAAMLSALGLVLVQRLASNLLAQQLTWTII
                     AAGAFMVTILIPRDLSVLARFKWTWALVGVALLVSPLLPVIGREVNGARIWIGVGPAN
                     FEPWEAVKILLVVFFAAYLEEYREVISRPRRIGPIALPPAPYLLPILAMWAIAMVVVV
                     FEKDIGAALLFLGIFLAMLYLATGEAFYTLIGLLLLLAGGFAVYQSFGHVQVRVDTWL
                     RPFAEDMRFDAGYQIVQGMFALGNGGLLGTGLGNGMPDRIPVVWSDFVFDAFAEETGF
                     VGALALLAAFLLLVYRGMLIAVRAPTPFLQLLAGGLSFIVAFQTLVIVGGNARLIPLT
                     GITLPFVAYGGSSLVTKWMLVALLIRVSDITARVRGTP"
     gene            8513..9949
                     /locus_tag="VFV20_05070"
     CDS             8513..9949
                     /locus_tag="VFV20_05070"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEU5287753.1"
                     /translation="MTRTIGANLRSLTLTIALAFLVTSVGVGYWTLVAADDLSSDPFN
                     PRLVAAVRDRPRGQIVDASGRVLAESVRTPSGYVRRYADRSLAQVVGFASFKYGTAGI
                     EAAYSDSLVGQDPADPLAAWRARYLGEREAPGQVVLGIDPKVQKAAVDALAGRRGAIV
                     ALDPRTGTILASVSFPNYDANQITDPATEKVAWDQVNGDAGKPLIDRARQGLYAPGST
                     FKLVTGAAAIESGFDPEQKVRVDDPWQADKSWGNYFVRSSSKAHGDYTMADGYRLSEN
                     IYFAKVGLQIGGQRLAEYAQRFGVGAAPRCDVTVAKGQLSATGALDRPTLIADTSYGQ
                     GELLTSPLQMALVAAAIGRGGVMPTPHYATEVRDSAGNTLRTIAPAPAGQVIRPDTAA
                     KVAAMLVGAVSGPGAFAFGAKISGVNVAGKTGTAENPSGVPHGWFVGFAPAENPVVAV
                     AVLIENSPQGGLDAAPLGGKVMQAALGR"
     gene            10049..11011
                     /locus_tag="VFV20_05075"
     CDS             10049..11011
                     /locus_tag="VFV20_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU5287754.1"
                     /translation="MDRAGLATRISSVAANVEKVIFGKHREVELALVALTCRGHILVE
                     DVPGTGKTVLAKAIARSLGCSFRRIQFTPDLLPSDVTGVSIFNQRTSTFEFRPGPIMS
                     QIVLADEINRATPKTQSALLEAMEEHQVTVDGTTYQLPEPFLVMATQNPIEYEGTFPL
                     PEAQLDRFFIRLQLGYPKDREEIAILDAQRVIHPLETIEQVLSAEELLEAQAAAKDIH
                     IADQVKAYVVAVVNATRSHPDVYLGASPRGSLALARASQAFAFLQGRDYVLPDDVKQL
                     AAPTLSHRLILQPQARLKDLAATTVIAEVLASVPVELSPTAARA"
     gene            11039..12250
                     /locus_tag="VFV20_05080"
     CDS             11039..12250
                     /locus_tag="VFV20_05080"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEU5287755.1"
                     /translation="MKTWHFAVLWLALFIIAASTGVELLSYLAYLLLFVGVAMWIQAR
                     LTLDGLSITRTISQTYAHLGDTFELVYELKNTSSFGKLWLEISEESNWPEPLPGRVLS
                     IGGGGKVRKWKVLAKAIRRGRYKLGPIVLRSGDPFGLFPREARVATEALLLVYPRVVP
                     LPHWQLPGSVLEGGVLTGQRSLQSTSMVMGIREYRSGDAMNRIHWPSSVRHRTLHVKE
                     FELDKTADLWIYLDLERHWHRGEGEDSTEERAVTVAASIVAKALREHRNVGLITSGPR
                     AEVFQPDRGTKQFGKVMQYLAEVRVAGSRTLAETLVETLPRLRRGSACILVTPSLDRA
                     WVRPASTLREAQVATQAVIVGSPAVGDGRDRARRDQLFGELAIAGVGATYLTPGASIS
                     DLFRDAVGAVA"
     gene            12243..>13139
                     /locus_tag="VFV20_05085"
     CDS             12243..>13139
                     /locus_tag="VFV20_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287756.1"
                     /translation="MRDVIGAWLARTVRALFPPISARRLRFRGGLFALPIYVLLVLIY
                     PLSLQQAEWVRTSEHFTWLAVIGILAGMLVGNGRMGARRAVLLAGILGAIAIVISTAI
                     ASEGSLLREKLVALAINVNNWLTQVLAGEAATDPTVFVLFLGATTWASAFVGTFALMR
                     TGRPWDALVFVGFCLVVNVSMALTNLLADLVVFTLGSLVLLVRLHIVALQDRWQRYNI
                     VPSGEMDWRLLRGGLTWSLVLVIMAFFTPRVGAAEVLGRAYNVFESPYHSVESEWQRF
                     FAGVSGPSRLRGVSFTDAIRLGQ"
BASE COUNT         1893 a         4508 c         4520 g         2218 t
ORIGIN      
        1 cgaggcgaag gacctcctcg tacggtcgcg cgagctcctc gctcggccgg ggttcgtcga
       61 gaaggccccg tccgcggtcg tcgcgaatga gaaggccaag ctcgccgagc gcgaggagcg
      121 tgttcgccta ctcgaggagg agctccgtcg tctgacctag gtcgcgctcc agtgtggcgc
      181 gacctcgagt tcaccaagct ctggagcggc tcgctgctct cggcggccgg cacggcgatc
      241 acggggctcg cgatgccgct gatcgccgtc gggcttggtg cgacgccggc gcagatgggc
      301 gtgctcggcg cgtccggcac ggcggcgttc atcgtcttcg gccttgccgc gggtgtcatc
      361 gtggaccgcg cgccgcgccg gtcggtcctc atgctcacga gcgcggggtc ggcggtcgtc
      421 gtcgcgaccg tgccgctcgc acatctcctg ggcctgctgc ggatcgagca gctctacgcc
      481 gtcgagttcg tggccggcag cctggcgctc gtggaccaga ccgcctttca ggccatcctt
      541 ccgcgcctgg tcgggcgcga acggctcctc gagagcatca cgctggtgcg ctccagcgac
      601 agcgttaccg ccgtcgccgg gccgagcatc gcgggcgtgt tggtgcaggt gctcggcgcg
      661 cccgtcgcga tcctcgtcga cgccgcctca tacctgctgc agacggtgct gacgttcctg
      721 gtgcgggtca cggaacccgc cgcgccggag cgcgcgccgg gcaggcgcgt gtggcacgac
      781 gtcgtcgaag ggctccgata cgtgctcgcc gagccgtcgc tccgcttcct cgcgctcggc
      841 ggcgcgacgc acaacgtgtg cagcaacggc gcgatcgtcg cgctgtacgt gctctacatg
      901 agccagcggc tttcgctctc gccggtcgag atcggcctcg tcttcgcggc cggcggaccg
      961 agcgcgctcc tcggctcggt gctggccggc cggtggggac gacgcttcgg catgcgcggg
     1021 gcgctctcgc aggcgcaggt gctcacgggc atcgcgcgcg gcttcgtgcc gctcgcatcc
     1081 ttcgtacctt cgccactctt ggccctcgtc gccggcgagc tcgtgctcgg catcgctcgc
     1141 tcggtgttca acgtgaacca gctctcgatg cgcatgacgc tgacgccgga ccatctgcag
     1201 gggcgcatga cggcgagcat ccgtttcctg atgtggagcg tcgttccggt cggcgcgctc
     1261 gccggcggat tcgcggcgga gcgattcggc ctcgtcccca cgctcgtcgt cgcggcgctc
     1321 ggcacggggc tgtcggcgct gtggttcctg ttcatccccc ctgaggagcg cgcggtctag
     1381 cccgactcgc gggaatcaaa agggcgccgg atcgctccgg cgccctttcg cgtgatcgag
     1441 gtcagcggtc tactgcgagt ccttcaggat ctggttcgcc ttgacgccgg cgtccttgat
     1501 cgcctgctcg ggcgtggcct tgccggtcac gaccgagaca tacatgtctt ggatcacgtt
     1561 gcggatctcc tgctgcccgc ggacgttcgg ctcggggatc gaattctgga tctggtcgta
     1621 ggcctgcttg ccctgcgggt ccgtcgtggt ccaggacttc ttcaggacct cgttgttcgc
     1681 ggctgccttt cgaacgggca tgtagaagga cttcgttgcc caatcggccg tctgctcctg
     1741 ctcactgaac cacttgatga acagccacga ggcgagctgc ttctgcggcg tgctcttgaa
     1801 taccgcgatg ttcgctccga acatcacggt cttgccgccc gggggaacgc tcacggacca
     1861 gttcaccggc gtcttcatcg ccgcggcgat gaacggacgg ctcgaggtcg atgagaaggt
     1921 gaatgcgagc ttgccctggg cgaagtcgtt ctgccaatcg aagcccgtgg gcgtgtacgc
     1981 gtacttgccg tcgtacagac gcttgagcat ctgaagcgtc gccaggcctt ccttgccgtc
     2041 ccatgcgacg gtcttcccgt cgtcggacat gagcttgccg ccacgcgaca gcaccatcgc
     2101 gttgaagtac gacgcgtcag acgtccccgc gtatccgtac tgggtggtct tgccgctcgc
     2161 gtccttcttc gtcgtcttca tgacggcctg ctcccactcg tcccaggtct tcggcacggt
     2221 ggcgacgccg gcggccttca gcgcgtcatc gttggcgtac atcaccgcga gcgacttggt
     2281 gaacgggaac gagaggagct tgttgccgta ctgcgcgaag cggttggtgt cgaagtacgg
     2341 cttgtagatg tcgtccttgc tcgcctggtc gaggccgttc ttcttgctgt tgacgaaggg
     2401 gtcgaggtcg atgacgacgt cggccttcgt gtagtccgcg acgttgctct catacgcatg
     2461 cgccatctcg ggcagcgcgc cggcctggat cgcgccgagg accttctggc cgagcacggt
     2521 gtagttgccc tgcacgatgg ccttcacggt gatgcccttg ccgttcgtgt cattgaactt
     2581 cttcaccgcg gcctcgagcg cgcccttctg cgggtcgctc gtgagcgcgt gccagagcgt
     2641 gacttccacc gggccggtga tctcgaggtc ggcttccgtg gtcgctgccg gtgtcggtga
     2701 ggtcggtgac ccgccgccgc aggccgcgat gagcatcgcg atggccgcga gaacgctgag
     2761 tttgcgcatt tgcccctgtc ctcctcaaac ctctccccac gactgtgcgt tgggcggtga
     2821 accgctgtat gcgatacgcg tacgcgttac ccgcggatgc ccgtcctcgc gaccccctgc
     2881 accagatagc gctgcccgac gagatacagc aggacgatcg gtgccacgac gaacgacgcc
     2941 gccgccatga gcagatggta cgacgagccc gcttccgctc ggaaggcatt gaggccgacc
     3001 tggatcggcc ggatgtctgg tgtcgatgtg acgatgagcg gccactggaa cgcgttccag
     3061 cttccgtaga aggcgatgat cgaaacggtg acgagtgccg gtaccgaaac gggtagcacc
     3121 accgaccaga ggaagcgcag atgtccggcg ccatcgatga tcgcggcgtc atacagctct
     3181 ctcggcacgg agaggaactg ctgccgcagc aggaagatcg agaaggccgt cgcgaggaac
     3241 ggcaggatct gcacccagta ggtgtcgtac agattgaggt accgcttgct cacgatcacg
     3301 aagttgggga tgagcgtcgc ctcgccgggc atcatgtagg tcgcgagaag gagcgcgaag
     3361 agcgcggccc tgcccttgaa gttcatccac gcgaacgcgt acgccgcgag gacgctcgtg
     3421 acgagctcgc cgagggtgtt cgagccggcc acgagcagcg tgttcgcgaa atagcgcgcc
     3481 cagggagcct tgttccagac ctcctggtag ttctcgagac gtacctcgtg gggccagagc
     3541 gtggggggca ccagtgtcgc ctcgaagaac ggcttcagcg acgagctgac catgaaatag
     3601 aaggggtacg ccacgagtcc gcctgcgacg atgagcacca ggtgcttggt gaggctcgag
     3661 gcgaccgccg gcacctgggg ccgccgcacc gccggtaccg acatgcgcgg gatcacggtg
     3721 gggacgttca tccgtactgc acccggcgct cgagcacacg gaactggatc agcgtgaatg
     3781 ccaggatgat gagcgtcagg agcacgccca tcgccgagcc gaggccgatc tggccctgct
     3841 ggaagaacgt ccggaagacg ttcatcgtca ccgtgcgcgt ggtgtcgagc ggaccaccgt
     3901 tggtgagcac atagatctcg ttgaatgcgc gaaggacgcc gatgctcgcg atggtgaaga
     3961 cgaagaagat ctgcggcgtg agcagcggga gcgtgatctt caggaacagc tgccgctgcg
     4021 acgcgccgtc cagcctggcc gcctcgtagt actgctcggg gatgttgccc aggccggcga
     4081 ggaagacgac cgtctggaat ccgacgaagt gccagatgga gaagagcgcg accgagacga
     4141 gggcgaggct cgggccggcg gcccactgcg gcaccgtcag gccgaccgtg ctgccgatca
     4201 tctcgaagat cccgtcgggc tcctgcagga accgcagcgg accgatcccc acgaggccga
     4261 gcacctggtt gaaggcgccg tactgggggt ggtacagcca ctcgaagacc gcggcggcgg
     4321 cgacggtggc ggtgatgtag gggagataga agatcgtccg gtagatcgcg cgaccacgga
     4381 tccgctgaaa gagcacgtac gcgatgacca gcgcgaagat gatctctaac gggatcgtga
     4441 tcgccgcgta cgtgaaggtt gtcgagaccg atcgccagaa ggactccgca cgtgagccga
     4501 acaggatgtc cgtgtagttg tcgatgccgc ggaaggcgcc ctggatgatg tcccacttga
     4561 agaagctgat gtagatcgcg aagaacgccg gccagatctt gaacaccgcg aggacggcga
     4621 atgccggcag caggaagaga taggcctgaa gggtctcgac gatgaagcgt cggcggcgcg
     4681 aggccgcgcg ctgcccggtc ccgatagccc ctcccgacac cttcggcgat gatatccagc
     4741 gtgcccatct acgactatcg ctgcgaacac tgcggccacg ccttctcggc gctgcagtcg
     4801 tacaacgacg acgcgctcgc gacgtgcccg agctgcggga agcagccacg caggctgatc
     4861 gcgccgccgg cgatcgtgtt caagggctcg ggctggtaca agaccgactc ccgggcgaag
     4921 cccgccgccg agacggcctc gcccgcggcg aaggacggag cgaaggacgg cgcgaaggac
     4981 ggagccaagt cggataccaa gtcggacgcg aagccggaca cgaagtcgga cacaaagtct
     5041 gaacccaagc cgtcggccgg ctcgtcggga aggtcgggcg ccgcatccta ggcgaccttc
     5101 gtcgcgcgag cggcgagatc cgcgccgctg gtgcgtcatt cctcggtgac gagtccgcgt
     5161 tcctcgcggg cggcgtggcc taccaggtgt tcttcgcgct catcccgatg ctcgcgctcg
     5221 tcgtcggcgt gctcgccttc gtctacgggc cggaccgggg ggagcgcgag ttcgcgcaga
     5281 tgctgcggca ggtgtatcca tccgcgacgg cgcaggaggt gcgcatcgcc cgcgagctcg
     5341 tggacggccg cgccatctcg ttcggattcg ggctcatcgg cacggtcctg tcggccggcg
     5401 cggtcttcgg ctcgatcgac accgcgctcg cgctcatcct gggtgcggga cggaggcgcg
     5461 gcctggtgcg cggccgcctc gacggcatgg ccttcatcgg cgccatcgcg ctcctcgcgg
     5521 tgctctcgtt cgcgctctcg tacggcgccc aggcggcgca gggcgcgttc gcggcggcgg
     5581 gcctggcgcg gaccggccgc ctgctgatcg cgctcggatc gtcgctcctc ggcctgctgg
     5641 cggggtggct cttcttctac gtgatctacc ggtacgtccc gcgggtgccc gtcgccccgc
     5701 gggtcgcccg gacggccgcc ctggtatcag ccgtgctgtg ggaggtcgcg aaagtggcgt
     5761 tcggcttctt caccggcggg ttgggggcct tcagcgccta cgggccgatc gcgttcgccg
     5821 cgggtcttct cacgtggatc tacctcacag cggtcatcat cctgttcgga gcgcagttca
     5881 tgaaggtgcg gatgcgagga gctggctaat ggaacgcgtc gaggctttcg tcagccgcgt
     5941 gatcgaggga tggtcggcgc gcctcttcgg cgccaagctg cagccggtcc agatcgcgaa
     6001 gcggctcatc cgcgcgatgg aggcgcacca gaccatcagc ctctcgaaga cattcgtccc
     6061 caactcgtac gtcgtgtccc tggggccgac ggacttcgcg cagttcgacc agtaccgccg
     6121 cagcctcgag cgggatctcg ccgagaccct gctgggggcg gcgcgcgaac ggaactacac
     6181 gctccttgcc tatcccaccg tcgagatcga acgcgacgac gacatgaccc cgggcgacat
     6241 ccgcgtctcg tgcgcgctcg tcgacgcttc cggcgaggag gtcgaggccg acgcgaagac
     6301 cgtgggcgcg gtcgagtcgg ggcacacgat ggtcctcgac cgggaggcgc tgctgcggga
     6361 gcgtccgcgc gcgccgcgcg cgtcgctgga gcgccgcgac gggccggccg tgccgctcgg
     6421 ccccgacccg gtcctcatcg gacgcgatcc ccagaacgac gtcgtcctcg acgatcgccg
     6481 cgtctcacgc aagcacgccg agatccgcct gcggctcggc cggtacacgc tgtacgacct
     6541 gcagagcacg aacggcacgt acgtgaacgg ccgccgcgtc gccgagaagg tcctcgagga
     6601 cggcgaccac gtcagcgtgg gcgggctcga gatcgtcttc cactcggccg attagcgatg
     6661 aagccgagcg aggagcgggt cccccgcgac gagcgagacc gacgcgcgag tgacaacgag
     6721 cggttgagca ggagcgagcg cgatagatgg agctgacctt cggcgcgctg ctctgggccg
     6781 tgcgcatcgc gttcctgctg ctcatctaca tcttcctcat ccgcgcgttc ggcgtcctcc
     6841 agcgcgcgct cgccgcggag cgcgtcgagg gaggcgccgt cgggacgctc gtggtggaac
     6901 gcagcccggc gcgcaccccg cgcgtcggcg agcgtcttcc gctgcgcgcg tcgaacgcca
     6961 tcggccggga cgcgggcaac gacatcgcgc tccccgacga ggcggcgtcc gcgcggcacg
     7021 cgacgctggc gctcgaggac ggggactggt ggatcgagga tctcggcagc acgaacggca
     7081 ccctcgtgaa cgggacgcgc atcaccaagc gcgagcggct gcggcccgga gacgagatcg
     7141 ccatcggccg cgtggcgctg cgcctcgagc gatgagggac cgcccgtgac cgctctgctc
     7201 ttccgccgct cgagcgagct cggtctgctc gtgtgggtgg cggccatcgc ggcggccggc
     7261 tcggtcgccg tcgcggcggc ggagacgtcc gccttcgacc gggcgcagct cgccataccc
     7321 gtcgtgttcg tcgcccttgc cgtcgcgctc cacgtcttcc tcgcggcgcg cggcatacgc
     7381 ggcgatcagc tgctcctgcc actcgccgcg atgctctcgg cgctcggcct cgtgctggtc
     7441 cagcgcctcg cgtcgaacct gctcgcgcag cagctcacgt ggacgatcat cgccgcgggc
     7501 gcgttcatgg tgacgatcct gatcccgcgc gacctcagcg tgctcgcgcg gttcaagtgg
     7561 acgtgggccc tcgtgggcgt cgcgctcctc gtttcgccgc tgctccccgt gatcggccgt
     7621 gaggtgaacg gtgcgcgcat ctggatcggc gtggggcccg cgaacttcga gccgtgggag
     7681 gccgtgaaga tcctgctggt ggtgttcttc gccgcctatc tcgaggaata ccgcgaggtc
     7741 atctcgcgac cgcgtcgcat cggacccatc gcgcttccgc ccgcgccgta cctgctgccg
     7801 atcctcgcga tgtgggccat cgcgatggtc gtggtggtgt tcgagaagga catcggtgcc
     7861 gcgctgctct tcctcgggat cttcctcgcg atgttgtacc tcgcgaccgg cgaggccttc
     7921 tacacgctca tcggcctcct gctccttctt gccggcgggt tcgcggtgta ccagtcgttc
     7981 ggtcacgtgc aggtgcgggt cgacacgtgg ctccggccct tcgccgagga catgcgcttc
     8041 gacgccggct atcagatcgt gcagggcatg ttcgccctcg ggaacggcgg cctcctcggg
     8101 accgggctcg gcaacggcat gcccgaccgc atcccggtgg tgtggagcga cttcgtcttc
     8161 gacgcgttcg ccgaggagac cggcttcgtc ggcgcgctcg cgctcctggc ggcgttcctc
     8221 ctgctcgtgt accgcgggat gctcatcgcc gtccgcgcgc cgacgccctt cctccagctg
     8281 ctcgccggcg gactctcgtt catcgtcgcg ttccagacgc tcgtgatcgt cggcgggaac
     8341 gcgcgcctca tcccgctcac cggcatcacg ctgccgttcg tcgcgtacgg cggatcgagc
     8401 ctcgtcacga agtggatgct cgtggcgctc ctgatacgcg tgtcggacat caccgcccgc
     8461 gtgcgaggca ccccgtgact ccgatccgtc ggcgcggcat cgaggcgctc ggatgacgcg
     8521 cacgatcggc gcgaaccttc gctcgctcac gctgaccatc gcgctcgcgt tcctcgtcac
     8581 gtcggtcggc gtcggctact ggacgctcgt cgcggccgac gacctctcga gcgatccatt
     8641 caatccgcgc ctcgtcgcgg cggtgcgcga ccggccgcgc ggacagatcg tcgacgccag
     8701 cggtcgcgtg ctggccgaga gcgtgcggac cccgagcggc tacgtgcgcc gctacgcgga
     8761 tcgctcgctc gcgcaggtcg tcggcttcgc gtcgttcaag tacggcaccg ccggcatcga
     8821 ggccgcgtat tccgattcgc tcgtgggaca ggacccggcg gatccgctcg ccgcctggcg
     8881 cgcgcgctac ctcggcgagc gcgaggcgcc cgggcaggtg gtcctcggca tcgatccgaa
     8941 ggtccagaag gccgcggtgg acgcgctcgc gggacggcgc ggcgcgatcg tcgcgctcga
     9001 tccgcgcacc ggcacgatcc tcgcgtcggt cagcttcccg aactatgacg cgaaccagat
     9061 caccgatccg gcgaccgaga aggtggcctg ggaccaggtg aacggcgatg ccggcaagcc
     9121 gctcatcgac cgcgcgcggc agggcctcta cgcgccggga tcgacgttca agctcgtgac
     9181 gggtgccgct gccatcgaga gcgggttcga tcccgaacag aaggtccgcg tcgacgatcc
     9241 ctggcaggcg gacaaatcgt ggggcaacta cttcgtgcgc tcgtcgtcca aagcgcacgg
     9301 cgactacacg atggccgacg ggtaccgcct ctccgagaac atctacttcg ccaaggtggg
     9361 cctgcagatc ggggggcagc gcctcgccga gtacgcgcag cgttttggcg tcggtgcggc
     9421 gcctcgctgc gacgtcacgg tcgcgaaggg acagctgtcc gcgacgggag cgctcgatcg
     9481 cccgaccctc atcgccgaca cctcctacgg acagggcgag ttgctcacat cgcccctgca
     9541 gatggcgctc gtcgcggcgg ccatcggccg gggcggtgtc atgcccacgc cgcactacgc
     9601 gaccgaggtt cgcgacagcg cggggaacac cctgcggacc atcgcgcccg cgccggcggg
     9661 ccaggtcatc cgcccggaca cggccgcgaa ggtcgcggcg atgctcgtcg gcgccgtctc
     9721 gggacccggg gccttcgcct ttggggcgaa gatctccggc gtgaacgtcg cgggcaagac
     9781 cggcacggcc gagaacccga gcggcgtgcc gcacggctgg ttcgtgggtt tcgccccggc
     9841 cgagaaccca gtcgtggccg tcgccgtatt gatcgagaac tcgcctcagg gagggctcga
     9901 cgcggcgcct ctgggcggga aagtgatgca ggccgccctc gggcgttgaa ccaagcggcg
     9961 acttcgcgcg ttcattccga taacaggtgt ttcgccttta gcatgggcgg gaagggagcc
    10021 cccaggggtt ctcatggaga ttcagctgat ggatagggcc ggcctcgcga cgcgcatctc
    10081 gagcgtcgcc gcgaacgtcg aaaaggtcat cttcggcaag caccgcgagg tcgagctggc
    10141 gctcgtcgcc ctcacgtgcc gcggccacat cctcgtggag gacgtccccg gcaccggcaa
    10201 gaccgtgctg gcgaaggcca tcgcgcggtc gctgggctgc tcgttccggc gcatccagtt
    10261 cacccccgac ctgctgccgt cggacgtgac cggcgtgtcg atcttcaacc agcggacgag
    10321 caccttcgag ttccggccgg ggccgatcat gagccagatc gtcctcgccg acgagatcaa
    10381 ccgggcgacg ccgaagacgc agtccgccct cctcgaggcg atggaggagc atcaggtgac
    10441 cgtcgacggt acgacctatc agctccccga gccgttcctc gtcatggcga cgcagaaccc
    10501 catcgaatac gaaggcacgt tcccgctgcc cgaggcacag ctcgaccggt tcttcatccg
    10561 gctccagctc ggatacccga aggaccggga ggagatcgcg atcctcgacg cgcagcgcgt
    10621 catccatccg ctcgagacga tcgagcaggt gctctccgcc gaggagctcc tcgaggcgca
    10681 ggccgccgcc aaggacatcc acatcgccga ccaggtgaag gcgtacgtcg tcgccgtcgt
    10741 gaacgcgacg cgctcgcatc cggacgtgta tctcggcgcc tccccgcgcg gcagccttgc
    10801 gctcgcgcgc gcctcgcagg cgttcgcgtt cctccaggga cgcgactacg tgttgcccga
    10861 cgacgtgaag cagctggccg cgcccacgct ctcgcaccgc ctcatccttc agccgcaggc
    10921 gcgcctgaag gacctcgcgg cgacgaccgt catcgccgaa gtgctggcga gcgtgccggt
    10981 cgagctctcg ccgaccgccg cgcgggcata gcggcgcgca tgcgccggcc gagctgacgt
    11041 gaagacctgg catttcgcgg tcctctggct cgcgctcttc atcatcgccg cgtccacggg
    11101 tgtggagctg ctcagctacc tggcgtacct gctcctgttc gtgggcgtcg cgatgtggat
    11161 ccaggcgcgc ctcacgctcg acggcctgtc gatcacgcgc acgatctcgc agacgtatgc
    11221 gcacctcggc gacacgttcg agctcgtgta cgagctgaag aacacctcgt cgttcggcaa
    11281 gctctggctg gagatcagcg aggagtcgaa ctggcccgag ccgctgcccg gacgcgtgct
    11341 gtcgatcggt ggcggcggga aggtgcgcaa gtggaaggtc ctggcgaagg ccatccgccg
    11401 cggccgctac aagctcgggc cgatcgtgct gcgctcgggc gatccgttcg gcctcttccc
    11461 gcgcgaagcg cgggtcgcca ccgaggcgct gctcctcgtg tatccgcgcg tcgtcccgct
    11521 cccgcactgg cagctgccgg gatcggtgct cgagggcggc gtcctcaccg ggcagcgcag
    11581 cctgcagtcg acctcgatgg tgatgggcat ccgcgagtac cgctcaggcg atgcgatgaa
    11641 ccggatccac tggccgtcgt cggtccggca tcgcacgctc catgtgaagg aattcgagct
    11701 cgacaagacc gccgacctct ggatctacct cgacctcgag cgccactggc accgcggcga
    11761 gggtgaggac tccaccgagg agcgtgcggt gaccgtggcc gcgtcgatcg tcgccaaggc
    11821 gctgcgcgag caccgcaacg tcgggctcat cacgagcggg ccgcgcgccg aggtcttcca
    11881 gcccgaccgc ggcacgaagc agttcggcaa ggtcatgcag tacctcgccg aggtccgcgt
    11941 cgccggctcg cgcaccctgg ccgagacgct cgtcgagacc ctgcctcggc tgcggcgggg
    12001 gtcggcatgc atcctcgtga caccatccct cgatcgcgca tgggtccgcc ccgcgagcac
    12061 gctgcgcgag gcacaggtcg cgacgcaggc ggtgatcgtc ggctcgcccg cggtcggcga
    12121 cggccgcgac cgcgcgcggc gcgaccagct cttcggcgag ctcgcgatcg ccggtgtcgg
    12181 cgcgacctac ctcacgccgg gcgcgtcgat cagcgatctc ttccgcgacg cggtgggcgc
    12241 cgttgcgtga cgtgatcggc gcatggctcg cgcgcacggt gcgcgccctt ttcccgccga
    12301 tctcggcgcg gcgcctgcgc ttccgcggcg ggctcttcgc gctgccgatc tacgtgctcc
    12361 tcgtgctcat ctacccgctc tcgctgcagc aggccgagtg ggtgaggacg tccgagcact
    12421 tcacctggct cgcggtcatc gggatcctcg ccggcatgct cgtgggcaac ggccgcatgg
    12481 gcgcgcggcg cgccgtgctt ctcgccggga tcctcggcgc gatcgcgatc gtcatctcga
    12541 cggcgatcgc gagcgaagga tcgctcctgc gcgagaagct cgtcgcgctc gccatcaacg
    12601 tcaacaactg gctcacgcag gtgctcgcgg gcgaggccgc gaccgatccc acggtgttcg
    12661 tgctcttcct cggcgcgacg acgtgggcct cggcgttcgt cggcacgttc gccctgatgc
    12721 ggacgggccg tccgtgggac gcgctggtct tcgtggggtt ctgcctggtg gtgaacgtgt
    12781 cgatggcgct cacgaacctg ctcgcggacc tcgtggtgtt cacgctcggc tcgctcgtgc
    12841 tcctcgtgcg cctgcacatc gtcgccctgc aggaccgctg gcagcgctac aacatcgtgc
    12901 ccagcggcga gatggactgg cgcctgctgc gcggcggcct cacctggtcg ctcgtgctcg
    12961 tgatcatggc gttcttcacg ccgcgcgtcg gcgcggccga ggtgctcggc cgcgcgtaca
    13021 acgtgttcga gtcgccgtat cacagcgtcg agtccgaatg gcagcgcttc ttcgccggcg
    13081 tctccggccc gagccgcctg cgtggtgtgt cgttcaccga cgcgatccgc ctcggtcag
//
LOCUS       DASTZR010000227         3763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1135601, whole genome shotgun sequence.
ACCESSION   DASTZR010000227 DASTZR010000000
VERSION     DASTZR010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135601"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="VFV20_05090"
     CDS             complement(<1..1128)
                     /locus_tag="VFV20_05090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEU5287757.1"
                     /translation="MSDPKALRKRALDAIDDRRDSLIELSLAIHANPELGYQERDAAL
                     RLTQYLEGDGFKVTHRYKGVETAYRADAKGKTDGPRVAILAEYDALADLGHGCGHNLI
                     AMIGTAAAVAVREVIEELPGSVAAIGTPAEEGGGGKVALLRAGGFDDVDAAMMIHPTT
                     GRNLAGRHSLASNRVVVEYFGRAAHAASQPDQGVNALDAMIQLFNGVNAMRQQLRPDA
                     RVHGIIAKGGSAANVIPEHTLGRFSVRALDRRYQQEVLKRFIACAEGAATATGTTVKV
                     TVDENAGYENMVFSTPMAERWAAHMRSLGAQVFDTRDDERVGSTDMGNVMQVLPGIHP
                     YVSISNDAIPGHSTAFRDHARTPEALERALVSSKALALTALD"
     gene            complement(1125..1778)
                     /locus_tag="VFV20_05095"
     CDS             complement(1125..1778)
                     /locus_tag="VFV20_05095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5287758.1"
                     /translation="MYGIPTSTTPTKREDWLTLGEASRMLGVDPDTLRRWADNGKIEV
                     FTTPGGHRRFLRASIEALLPRARPARRPSLSAMGEAPDRVASEFRKRVRTELSDQDWR
                     ARFDERSLRWFRERGMRMSDLLLGSLDTSRRAGREQFLTQAEALGREYGAAARSAGLS
                     LGEATQAFLFFRARFMAEIAQVARRRSLETAQAATLFAEADGALDRIILALIAGHQA"
     gene            complement(1789..3045)
                     /locus_tag="VFV20_05100"
     CDS             complement(1789..3045)
                     /locus_tag="VFV20_05100"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5287759.1"
                     /translation="MFRGPLRHRWFAAFIAAHGLGSVADEVARLALPLLILDLTHSIA
                     AAAALRIVQSIPYILFGAPAGALIDRTDKRRLLIACDIASGVLTVTIPLSAILGFFSV
                     EFLYVIGFLLGTIEVLWGVTTDFSVVPALVEADELTEANAIFFGADRFARVLGPTAGG
                     LAIAAVGSVSAMWIAALAFVPTLALFWRMPPLLDTERGARAPLTVVNVMKEIGDGFRY
                     VWRDPVLRSLLVVMSCANLGGVGIRTLMLYVLREEQHLDEVTIGIALSASGLLLVASS
                     VFVPRFTRDRPMGQSMIASAAIAAVAAMLTALTHDWRFIMVGFTAREIAWQAFIIYAF
                     IPRQRVPAQVRGRANGAFRTIVLVSNSASPAVLSAIVVVASSAAAFAAAGVLMLAGAA
                     IGAVTPLRSYAARESAEPHATLDSPA"
     gene            3135..3317
                     /locus_tag="VFV20_05105"
     CDS             3135..3317
                     /locus_tag="VFV20_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287760.1"
                     /translation="MGQVNVNPGGTSGDGGFGAGMIIGIILLILIVLVLIFYAGPQIF
                     SPGTTPRSFIDVLSIA"
     gene            3379..>3763
                     /locus_tag="VFV20_05110"
     CDS             3379..>3763
                     /locus_tag="VFV20_05110"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEU5287761.1"
                     /translation="MKPKRVLFLCSHNSARSQMAEAFLRAIAPDRFAVASAGTKATRV
                     HPLAIAAMREVGIDISGQRSKSVDEVGEGWDVVVTVCDAHCPVPPRSGMRLSWRFPDP
                     SAATGTDEEKLAAFRHVRDGIHSRVV"
BASE COUNT          576 a         1298 c         1344 g          545 t
ORIGIN      
        1 gtcgagcgcc gtgagcgcga gcgccttgct cgacacgagc gcgcgctcca gggcctcggg
       61 ggtccgggcg tgatcgcgga acgcggtgga gtgtcctggg atcgcgtcgt tggagatcga
      121 gacgtagggg tggatgcccg gcagcacctg catcacgttg cccatgtccg tcgagccgac
      181 gcgctcgtcg tcacgcgtgt cgaacacctg tgccccgagc gagcgcatgt gggcggccca
      241 gcgctccgcc atcggcgtcg agaagaccat gttctcgtag cccgcgttct cgtccacggt
      301 caccttcacg gtggtgcctg tcgccgtcgc ggcgccctcg gcgcacgcga tgaaccgctt
      361 cagcacctcc tgctggtagc ggcggtcgag tgcacggacg ctgaagcggc ccagcgtgtg
      421 ctcggggatg acgttggccg cgctgccgcc cttcgcgatg atgccgtgca cgcgcgcgtc
      481 cggccgcagc tgctggcgca tcgcgttgac gccgttgaag agctggatca tcgcgtcgag
      541 cgcgttgacg ccctgatccg gctgggacgc ggcgtgcgcg gcccggccga agtactcgac
      601 gacgacgcga ttcgacgcga gcgagtgccg gccggcgagg ttccggccgg tggtcggatg
      661 gatcatcatc gccgcgtcca cgtcgtcgaa cccgcccgca cgcagcagcg cgaccttgcc
      721 gccgccgccc tcttccgcgg gcgtgccgat cgccgcgacg ctcccgggca gctcctcgat
      781 gacctcgcgc accgccaccg cggccgcggt gccgatcatc gcgatcaggt tgtgcccgca
      841 gccgtggccg aggtccgcca gcgcgtcgta ctcggcgagg atcgcgacgc gcgggccgtc
      901 cgtcttgccc ttcgcgtcgg cgcgataggc ggtctcgacg cccttgtagc ggtgcgtcac
      961 cttgaagccg tctccctcga ggtactgcgt gaggcggagc gcggcgtccc gctcctggta
     1021 gcccagctcg gggttggcat ggatcgcgag cgacagctcg atgagcgagt cgcgacggtc
     1081 gtcgatcgcg tcgagcgcgc gcttgcgcag cgcctttgga tcgctcatgc ctgatggcca
     1141 gcgatgagcg ccaggatgat gcggtccaac gcgccgtccg cctcggcgaa gagcgtcgcc
     1201 gcctgcgcgg tctcgagcga gcggcggcgg gcgacctgcg cgatctccgc catgaaccgt
     1261 gcgcggaaga agaggaatgc ctgcgtcgcc tcgccgagcg acaggcccgc ggatctcgcg
     1321 gccgctccat attcgcgtcc gagagcctct gcctgcgtga ggaactgttc gcgccccgcg
     1381 cgccgcgagg tgtcgagcga cccgaggagg agatcgctca tgcgcatgcc gcgctcgcgg
     1441 aaccagcgca gcgaccgctc gtcgaatcgc gcccgccagt cctgatcgct gagctcggtg
     1501 cgcacgcgct tccggaattc cgacgcgaca cgatcgggtg cctcgcccat cgcggagagc
     1561 gacggccgcc gcgccgggcg cgcgcggggc agcagcgcct cgatcgaggc gcgcaggaag
     1621 cggcggtgcc cgcccggggt ggtgaagacc tcgatcttcc cgttgtcggc ccagcggcgc
     1681 agcgtgtcgg ggtccacgcc gagcatccgg ctcgcctcgc cgagggtgag ccagtcctca
     1741 cgcttcgtgg gagtggtcga tgtggggatg ccgtaaatct aggcgaacct acgcggggct
     1801 gtccaacgtt gcgtgaggct cggcgctctc gcgcgcggcg taggagcgca gcggcgtgac
     1861 cgcaccgatc gccgcgcccg ccagcatcag cacgccggcc gccgcgaacg ccgccgcgct
     1921 cgaggccacg accacgatcg ccgacagcac ggcgggtgac gcgctgttgg acacgagcac
     1981 gatcgtccgg aaggccccat tcgcgcggcc gcgcacctgc gccgggaccc gctggcgcgg
     2041 gatgaacgcg tagatgatga aggcctgcca cgcgatctcg cgcgcggtga acccgaccat
     2101 gatgaagcgc cagtcgtgcg tcagcgccgt cagcatcgcc gcgaccgcgg cgatggccgc
     2161 gctggcgatc atcgactggc ccatcggccg gtcccgcgtg aagcgcggga cgaagaccga
     2221 gctggcgacg agcagcaggc ccgacgcgga gagcgcgatg ccgatggtga cctcatcgag
     2281 gtgctgctcc tcacgaagca cgtacagcat cagcgtgcgg atgccgacgc ctcccaggtt
     2341 cgcgcacgac atcacgacga gcagcgaccg cagcacgggg tcgcgccaga cgtagcgaaa
     2401 gccgtccccg atctctttca tgacgttgac gaccgtcagc ggggcgcgcg cgccgcgttc
     2461 ggtatcgagc agcggcggca tgcgccagaa cagcgcgagc gtgggcacga aggcgagcgc
     2521 ggcgatccac atcgcgctca cgctgccgac ggccgcgatg gcgagcccgc ccgcggtcgg
     2581 tccgaggaca cgtgcgaagc ggtcggcgcc gaagaagatc gcgttcgcct cggtgagctc
     2641 gtccgcctcg acgagggccg gtacgacgct gaaatcggtg gtcacgcccc agaggacctc
     2701 gatcgtcccg aggaggaatc cgatcacgta gaggaactcg accgagaaga agcccaggat
     2761 cgccgagagc gggatcgtca ccgtgaggac gccactcgcg atgtcgcagg cgatgaggag
     2821 ccgtcgcttg tccgtccggt cgatgagcgc gcctgccggc gcgccgaaga ggatgtaggg
     2881 aatcgactgg acgatccgca gcgcggccgc ggccgcgatg ctgtgtgtca ggtcgaggat
     2941 gaggagcggc aaggcaaggc gcgcgacctc atccgcgacc gagccgaggc cgtgcgcggc
     3001 gatgaacgcg gcgaaccagc ggtggcgcag cggccctcgg aacacgggag agcgagcata
     3061 agagcaagcc cgtgcccgcg gcatggcgct tgcgagagag caggacgact gaccgagagg
     3121 aggtcgtacc gcatatgggc caagtgaacg tgaatcccgg cggcacaagc ggcgacggtg
     3181 ggttcggcgc gggcatgatc atcggcatca tcctgctcat cctcatcgtc ctcgtgctca
     3241 tcttctacgc cggcccgcag atcttcagcc ccggcaccac gccgcgttcc ttcatcgacg
     3301 ttctttccat cgcctagccg acgtgggctc gcgaggcggc gccgggcatg accggcgccg
     3361 cttcgccatg cttagcgcgt gaagccaaag cgcgtcctct tcctctgctc gcacaactcc
     3421 gcgcgctctc agatggccga agcattcctc cgagcgatcg cgcccgatcg tttcgcggtg
     3481 gcgagcgcgg gaacgaaggc gacgcgcgtg catccgctcg cgatcgcggc gatgcgtgag
     3541 gtcgggatcg acatcagcgg ccagcgatcg aagagcgtgg acgaagttgg cgagggatgg
     3601 gatgtggtgg tcaccgtctg cgacgcgcac tgtccggtcc cgccgcgatc cggtatgcgg
     3661 ctctcctggc gctttcccga cccgtcggcc gccacgggca cggacgagga gaagctcgcc
     3721 gcgttccgcc acgtgcgcga tggcatccac tcgcgcgtcg tgc
//
LOCUS       DASTZR010000228         6825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1211247, whole genome shotgun sequence.
ACCESSION   DASTZR010000228 DASTZR010000000
VERSION     DASTZR010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6825
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211247"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2115)
                     /locus_tag="VFV20_05115"
     CDS             complement(<1..2115)
                     /locus_tag="VFV20_05115"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5287762.1"
                     /translation="MRTSVRFPARTSLEQILDGLARDAAFQEMVTRWERIPARPARYM
                     PFPEWLDGRIGAALRRRGITELYSHQVAALESTRAGRHTVIVTPTASGKTLCYNLPVL
                     DAIAKDPTARALYIFPTKALSQDQLAEIERESTDMDIELKTYTYDGDTSPAVRAAIRS
                     AGHVVITNPDMLHTGILPHHTKWVKLFENLRYVVLDELHIYRGVFGSHVANVLRRLRR
                     VCEFYGSHPVFICTSATIANPEELARKHIEDDVVVIDESGAPRGEKVLVFVNPPVVNR
                     NLGVRKSALITGRDIAATLLASGVQTIAFARSRVQTELLLTYLRSRFPQPQWPSDLIR
                     GYRGGYLPSERRAIERGLRDGSVRGVVSTNALELGIDIGALQAAVLLGYPGTVASTWQ
                     QMGRAGRREDLSAAFLVATSSPTDQYVVQHPEYVLDRSPEAGLVNPDNLHVLVQHLKC
                     AAFEIPFEARERFGTEDTPGVLAYLDEQGVLHEAGGKYHWSDQAFPAEAMSLRTATND
                     NVVIVDTTGPAPRTIGSEYHDGKARIIGEIDRFAAQVLLHEQAIYLHESRQLQVERLD
                     WENAKAYVREVKVDYYTQAELAVRIRVIDEFDRRSASRGFDLAHGEVLVSTLTSLYKK
                     LTMYTHENVGWGKIHLPEQELQTTSFWCSLPERAVAGWPKERVEIALAGLGNLLHGLA
                     PLLLMCDPHDLGLAVEVRSPHTE"
     gene            complement(2256..5426)
                     /locus_tag="VFV20_05120"
     CDS             complement(2256..5426)
                     /locus_tag="VFV20_05120"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU5287763.1"
                     /translation="MTTEQRKLVSVVFADVVGSTAFGSDNDPEAVRSVMGSYFERMKA
                     IAEMHGGTVEKFIGDAVMIVFGVPRLHDDDAERAVRAALAMRDAMADLNRELGVTLAA
                     RIGVNSGEAVAGSGVERQFLVTGDAVNVAARLQQGAEPGEVVVGALTESLTRSAIEYA
                     PRDAVAAKGKSEPLGAFRAVRPRSAVPEQARGLPQMRAQLVGRQRELRLLLDTFERVR
                     TERRAHLFTLVGNAGIGKSRLVGEVLARVASGGGARVLRGRCLPYGAGITYWPLMEIV
                     REDAGIASDDDRETAIAKLDRRTAEVAEATAASLRNRLAAMLGLRTVEEAIPEVRAPD
                     VSGEIAWAVREYLVAAAREPLVVVVDDLQWAEPAVFDIVEGVAERAEDAPLLLLCVAR
                     PTLLETHPGWAAGRANAATITLDALSADETTTLIARLLDVDDLPDDLRARVVERSEGN
                     PLFCEEFLRMLIDEGRVVRVEDRWRAAPGAADVRVPESIHALIAARIDGLGEDERRVI
                     QIASIVGERFAVSELRALAPDLDVAPALTGLRRAGIVLEDRETREPGHYRFKHLLMRD
                     VAYAGMSKAARADLHEAFARELERSVGDRREEFAEILAYHAERAFTLSVEVRAARAVI
                     EPRARVVLDNALQLGERARRRRDLGLIRPHSAAATAALAALGAAASAEDRLSVQLLQA
                     NEQLLNTSYREAVASFTDATRVADELGRKDLSAQARLGAAGAYVWSAGSAAEIEALVR
                     NTEEAAQQFRELGDTGSEIECGLIALDADWASGDLPRLLEKGRILRERAREIGDGARE
                     LLLCARLAPAAGQEDRWDLFGEYIARANELVARLGARAPSWLRVTPCGKLRSDGDLNA
                     ALACLEQVARDGRVEHDPTLEMPLLRGGAEIEAIERRRYTEAAAYADKGLELSVRTGE
                     RWNRAELTGTRAICTAASGDVAGAERQLADAYALADRGDVFAIAHITHCKARVLELAG
                     RDADAEAAFGDAERHYAQSGYTRGMWHAQVLLDLAHFLADRGRAAEAQERLDQAVALF
                     TPQVGERAERIAKLRERIAAARAQPA"
     gene            complement(5457..>6825)
                     /locus_tag="VFV20_05125"
     CDS             complement(5457..>6825)
                     /locus_tag="VFV20_05125"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287764.1"
                     /translation="LGARLGDAAPDEIVGYHFEQAAQYRRELGAPDAALGARAADRLW
                     AAARAARAREDVAAAANLLGRTAALLAESDPRLASALVEHGEALRQLGELPLAGDVFA
                     RSIALAHAVNESAVEWRARIGVAAVRIWREPEGAAQLALDEGNAAIAALPDDEHDVLA
                     RAWILLGDAALLRSQMAERMRTSEQALRHARAAGDRALEVEIIKNLAPAMLFGDVSVE
                     DGMRYVDDVLARLGDVPGVRSFGLHVMGHLRARRGEFAEARQAIEEWRGHMKELGRIA
                     QHANTAGCAYDVLSLAGDWSAAEREVREASQTLERMGDRASRSTTVAYLGVACFEQGK
                     IDEADRCATLSGELGASDDAMNEAIWRSLRARVLAARGEREEAVAQARKAVEVADRTD
                     YIDLQGDARRDLAIVLGAGPDGVRALDEAIARYERKGNRVSADGARALRLRMSGGPER
                     AAQRQ"
BASE COUNT          934 a         2430 c         2449 g         1012 t
ORIGIN      
        1 ctcggtgtgc ggcgagcgca cctccaccgc cagtcccagg tcgtgcgggt cgcacatgag
       61 gaggagcggc gcgagcccgt gcagcaggtt gccaagaccg gcgagggcga tctcgacgcg
      121 ctccttcggc catcccgcga cggcgcgctc gggcagcgag caccagaacg aggtggtctg
      181 cagctcctgc tccgggagat ggatcttccc ccagccgacg ttctcgtgcg tatacatcgt
      241 cagcttcttg tagagagagg tgagcgtcga cacgaggacc tcgccgtgcg cgaggtcgaa
      301 cccgcgcgac gccgagcggc ggtcgaactc gtcgatgaca cggatgcgca cggcgagctc
      361 cgcctgggtg tagtagtcga ccttcacctc gcgcacgtag gccttggcgt tctcccagtc
      421 gaggcgctcg acctggagct gtctggactc gtggaggtag atggcctgct cgtgcagcag
      481 gacctgcgcg gcgaagcggt cgatctcgcc gatgatgcgc gccttgccgt cgtgatattc
      541 ggagccgatg gtgcgcggcg ccggaccggt cgtgtcgacg atgacgacgt tgtcgttcgt
      601 ggccgtgcgg agcgacatcg cctcggcggg gaatgcctga tccgaccagt ggtacttgcc
      661 gcccgcctcg tggagcacgc cctgctcgtc gaggtaggcg agcacgccgg gcgtgtcctc
      721 ggtgccgaag cgctcgcgcg cctcgaaggg gatctcgaac gccgcgcact tcaggtgctg
      781 cacgaggacg tggaggttgt ccgggttcac gaggcccgcc tcgggcgagc ggtcgagaac
      841 gtactcgggg tgctgcacca cgtactggtc cgtcggcgac gaggtggcga cgaggaatgc
      901 cgcggagaga tcctcgcggc gtccggcgcg gcccatctgc tgccacgtgg aggcgaccgt
      961 tcccggatag ccgaggagca ccgcagcctg cagcgcgccg atgtcgatgc cgagctcgag
     1021 cgcgttcgtc gagacgacgc cgcgcacgga cccgtcgcgc aggccgcgct cgatggcgcg
     1081 gcgctccgag ggcaggtagc cgccgcgata gccccggatg aggtcggacg gccactgcgg
     1141 ctgggggaag cgagagcgaa ggtaggtgag caggagctcg gtctgcacgc gcgagcgcgc
     1201 gaacgcgatg gtctgcacgc ccgaggcgag gagcgtcgcc gcgatgtcgc ggccggtgat
     1261 gagcgcgctc ttgcgcacgc cgaggttccg gttcacgacc ggcgggttga cgaacacgag
     1321 gaccttctcg ccgcgcggcg cgcccgactc gtcgatgacg acgacgtcgt cctcgatgtg
     1381 cttgcgcgcg agctcctccg ggttggcgat cgtcgcggac gtgcagatga agaccggatg
     1441 cgatccgtag aactcgcaga cgcggcgcag ccgacgcagt acgttggcga cgtggctgcc
     1501 gaacacgccg cggtagatgt gcagctcgtc gaggacgacg tagcggaggt tctcgaagag
     1561 cttcacccac ttggtgtggt ggggaaggat cccggtgtgg agcatgtccg ggttcgtgat
     1621 gaccacgtgc cccgcgctgc ggatcgccgc gcgcaccgcc ggcgaggtgt cgccgtcgta
     1681 cgtgtacgtc ttgagctcga tgtccatgtc ggtggactcg cgctcgatct cggcgagctg
     1741 gtcctgcgag agcgccttgg tggggaagat gtagagcgcg cgtgccgtcg ggtccttcgc
     1801 gatcgcgtcg aggaccggca ggttgtagca gagcgtcttg cccgacgcgg tcggcgtgac
     1861 gatgaccgtg tggcggccgg cacgcgtcga ctcgagcgcc gcgacctggt gcgagtacag
     1921 ctcggtgatg ccgcggcgcc gcagcgcggc gccgatgcgt ccgtcgagcc actccgggaa
     1981 gggcatgtac cgcgcgggcc gcgccgggat gcgctcccag cgcgtgacca tctcctggaa
     2041 cgccgcgtcg cgcgcgaggc cgtcgaggat ctgctcgagg ctcgtgcggg cggggaaccg
     2101 aacggacgtt ctcatggagg catcgcacga tatggaacgt gcgttcgaca gtcaagagac
     2161 cctggagttc gcgtctgaga gatcccgcca atacggggca gcgcagccgg gctcgtgtac
     2221 cgagaaatcg gtatacgagc gagcgagcta gggctctacg cgggctgcgc ccgtgcggcg
     2281 gcgatgcgct cgcggagctt cgcgatacgt tcggcgcgct cgccgacctg tggcgtgaac
     2341 agggctaccg cctggtcgag ccgctcctgt gcctccgccg cgcggccgcg gtcggctagg
     2401 aagtgcgcga ggtcgagcag cacctgcgcg tgccacatcc cgcgcgtgta gccggactgc
     2461 gcgtagtgcc tctcggcgtc gccgaaggcc gcctcggcgt cggcgtcgcg gcccgcgagc
     2521 tcgagcacgc gcgccttgca gtgcgtgatg tgcgcgatgg cgaagacgtc gccgcgatcg
     2581 gcgagcgcgt aggcgtcggc gagctgccgc tccgcacccg cgacgtcgcc gctcgctgcc
     2641 gtgcagatcg cgcgcgtgcc cgtgagctcg gcgcggttcc agcgctcgcc ggttcgcacg
     2701 ctcagctcga ggcctttgtc ggcgtacgcc gccgcctcgg tgtagcgccg ccgttcgatc
     2761 gcctcgatct cggcaccgcc acggagcagg ggcatctcga gcgtggggtc gtgctcgacg
     2821 cgcccgtcgc gcgcgacctg ttcgaggcag gcgagcgcag cgttgaggtc gccatcgctg
     2881 cgcagctttc cgcagggcgt cacgcgcagc catgacggcg cgcgcgcgcc cagccgggcg
     2941 acgagctcgt tggcgcgagc gatgtattcc ccgaagaggt cccatctgtc ttcctggccc
     3001 gcggccggcg cgaggcgtgc gcagagcagc agctcgcgcg cgccgtcgcc gatctccctc
     3061 gcccgctcgc gtaggatgcg ccccttctca aggagtcgcg gcagatcgcc gctcgcccag
     3121 tccgcgtcga gcgcgatgag gccgcactcg atctcgctcc cggtgtcgcc gagctcgcgg
     3181 aactgctgcg ctgcctcctc cgtattgcgg acgagcgcct cgatctctgc ggcgctcccg
     3241 gcgctccaga cgtacgcgcc cgccgcgccg agacgcgcct gcgcgcttag atcctttcgg
     3301 ccaagctcat ccgcgacgcg cgtcgcgtcc gtgaagctcg ccacagcctc gcgataggac
     3361 gtgttcagca gctgctcgtt cgcctggagc agctgtaccg agaggcgatc ctcggcagac
     3421 gccgcggcgc cgagcgccgc gagcgccgct gtcgcggcgg cgctgtgcgg gcggatgagc
     3481 ccgagatcac gacggcgtcg cgcacgttcg ccgagctgta gcgcgttgtc gaggacgacg
     3541 cgcgcccgcg gttcgatcac tgcacgcgcc gcgcgcactt cgactgagag cgtgaacgcg
     3601 cgctccgcgt ggtacgcgag gatctcggcg aactcctcgc gtcggtcgcc gacgctccgc
     3661 tcgagctcgc gcgcgaacgc ctcgtgcaga tccgcgcggg cggccttcga catcccggcg
     3721 tacgcgacgt cgcgcatgag caggtgcttg aagcggtagt gcccgggctc gcgcgtctcg
     3781 cgatcctcga gcacgatgcc ggcgcggcgc agccccgtga gcgccggagc gacatcgagg
     3841 tcgggcgcga gcgcacgaag ctccgacacc gcgaagcgct cgccgacgat cgacgcgatc
     3901 tggatgacgc gccgctcatc ctccccgagc ccgtcgatgc gcgccgcgat gagcgcatgg
     3961 atgctctccg gcacgcgcac gtccgcggcg ccgggcgccg cgcgccagcg atcctcgacg
     4021 cggaccacgc gtccctcgtc gatgagcatg cgcaggaact cctcgcagaa gagcgggttg
     4081 ccctcggacc gctcgacgac gcgcgcgcgg aggtcgtcgg gcaggtcgtc gacgtcgagg
     4141 aggcgcgcga tgagcgtcgt cgtctcgtcg gccgaaaggg catcgagcgt gatcgtcgcg
     4201 gcgttcgcgc gtccggcggc ccacccggga tgcgtctcga ggagcgtcgg ccgtgcgacg
     4261 caaagcagca ggagcggcgc gtcctccgcg cgctccgcga cgccttcgac gatgtcgaac
     4321 acggcgggtt cggcccactg caggtcgtcg acgaccacga cgaggggctc gcgcgcggcg
     4381 gcgacgagat actcgcggac ggcccacgcg atctcgccag agacgtcggg tgcacggacc
     4441 tctgggatcg cctcctcgac ggtgcgcagg ccgagcattg ccgcgagacg attccgcagg
     4501 gacgcggccg tagcctcggc gacctccgcc gtgcggcgat cgagcttcgc gatggccgtc
     4561 tcgcggtcgt cgtccgaggc gatcccggcg tcctcgcgca cgatctccat gagcggccag
     4621 taggtgatgc ccgcgccgta cggcaggcag cggccgcgaa gcacgcgggc gccgccgccg
     4681 ctggccacac gcgcgagcac ctcgcctacc aggcgcgact tcccgatgcc ggcgttgccg
     4741 accaaggtga agaggtgggc gcgccgctcg gtgcgcacgc gctcgaaggt atctaggagc
     4801 aggcgcagct cgcgctggcg tccgaccagc tgcgcacgca tctgcggaag cccgcgcgcc
     4861 tgctcgggca ccgcgctgcg cgggcggacc gcgcggaacg ccccaagcgg ctcgctcttg
     4921 cccttcgcgg cgacggcatc gcgcggcgcg tactcgatcg cggagcgggt gagcgactcg
     4981 gtgagcgcgc cgacgacgac ctcgcccggc tcggcgccct gctgcaggcg cgccgcgacg
     5041 ttcaccgcgt cgccggtgac gaggaactgg cgctccacgc ccgagcccgc gacggcctcg
     5101 cccgagttca ccccgatgcg cgccgcgagg gtcacgccga gctcgcggtt cagatcggcc
     5161 atcgcgtcgc gcatggcgag tgccgcacgg acggcgcgct cggcgtcgtc gtcgtgcagg
     5221 cgcggcacgc cgaagacgat catcaccgcg tcgccgatga acttctcgac ggtgccgccg
     5281 tgcatctcgg cgatcgcctt catgcgctcg aagtacgagc ccatgacgct gcgcaccgcc
     5341 tcggggtcgt tgtccgaccc gaacgcggtc gagccgacga cgtccgcgaa cacgacggag
     5401 acgagcttgc gttgctcggt ggtcatgctg tggtcctgtg cgctcggatc acgccgctat
     5461 tgtcgctgcg cagcgcgttc gggcccgccg ctcatccgca gccgcagcgc ccgcgcgccg
     5521 tctgcgctca cccgattccc cttgcgctcg tagcgtgcga tggcctcgtc gagtgcgcgt
     5581 acgccgtcgg gaccggcgcc gagcacgatg gcgagatcgc gccgggcatc gccctgcagg
     5641 tcgatgtagt cggtgcggtc ggcgacctcg actgccttac gcgcctgcgc cactgcctct
     5701 tcgcgctcgc cgcgggccgc gagcacgcgc gcgcggagcg agcgccagat agcctcgttc
     5761 atcgcgtcgt cgctcgcgcc gagctcgccg ctgagcgtcg cgcaacggtc cgcttcgtcg
     5821 atcttcccct gttcgaagca ggcgacgccg aggtaggcga ccgtcgtcga tcgcgatgcc
     5881 cggtctccca tgcgctcgag cgtctggctc gcctcacgca cctcgcgctc ggcggccgac
     5941 cagtcgcccg cgagcgacag cacgtcgtac gcgcaccccg cggtgttcgc gtgttgcgcg
     6001 atgcggccga gctccttcat gtgcccgcgc cactcctcga tcgcctggcg cgcctccgcg
     6061 aattcgcccc gtcgcgcgcg cagatgaccc atcacgtgaa gcccgaacga ccgcacgccg
     6121 ggcacgtctc cgaggcgcgc gagcacatcg tcgacgtagc gcatgccgtc ctcgaccgat
     6181 acatcgccga agagcatggc gggcgccagg ttcttgatga tctccacctc cagggcgcgg
     6241 tcgcccgcgg cgcgagcgtg gcgcagcgcc tgttccgacg tgcgcatgcg ctcggccatc
     6301 tggctgcgca gcaacgccgc gtcaccgagc aggatccacg cgcgcgcgag cacgtcgtgc
     6361 tcgtcgtccg ggagcgcggc gatcgcggcg ttcccctcgt ccaaggctag ctgtgcggca
     6421 ccctcgggct cgcgccagat acgcaccgcc gcgacgccga tccgagcgcg ccactcgacg
     6481 gcgctctcat tgacggcgtg cgcgagcgcg atcgatcggg cgaagacgtc gcccgccagc
     6541 ggaagctcgc cgagctggcg cagcgcctcg ccgtgctcga cgagcgcgct cgcgaggcgt
     6601 gggtcgctct ccgcgagcag cgccgccgta cgtccaagaa gattcgccgc tgctgcgaca
     6661 tcctcgcgcg ctcgcgcggc tcgcgctgcc gcccagagcc tgtccgccgc acgggcaccg
     6721 agcgcagcgt ccggcgcccc gagctctcgc cggtactgcg cggcctgctc gaagtgatac
     6781 ccgacgatct catccggtgc ggcgtcaccg agacgagctc cgagc
//
LOCUS       DASTZR010000229         2632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1057502, whole genome shotgun sequence.
ACCESSION   DASTZR010000229 DASTZR010000000
VERSION     DASTZR010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057502"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="VFV20_05130"
     CDS             complement(<1..1176)
                     /locus_tag="VFV20_05130"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HEU5287765.1"
                     /translation="MGKTVAEKILARKSGRESVTPGEIVWCGVDLARVHDSSGPRRLG
                     PMLERLGAQIWDPSKLVIATDHFVPANDQTEVEIARVTREFAHDRGIAKYHEAEGICH
                     IVPLERGYVRPGMLFVGADSHSTTAGVMGAFAVPVGVTEMLGVLVTGEIWLKVPVTTR
                     IEWTGRLAEGCMAKDMVLKVLGEIGDDGATYHALEYGGEAVYALPFDERIVLTNMAIE
                     HGAKTGIIPPDELVFDLVGGAGREDAAYPDPDATYARVVRNRADELVPLVAYPGSPDN
                     VAPATDARGEKIVQAYIGACTGAKYTDLAAVARVVKGRHVADGIRFLIAPASKDVTRR
                     AAESGVLATILEAGGTLLPTGCGACAGLGHGILGKGERCISSTNRNYPGRMGHVEAEV
                     "
     gene            complement(1179..>2632)
                     /locus_tag="VFV20_05135"
     CDS             complement(1179..>2632)
                     /locus_tag="VFV20_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HEU5287766.1"
                     /translation="EAVTERYDLLVRGGTAVLPDVGPVRADVAARDGVIVAIGDDLPV
                     TAAERVIDARGKLVLPGAIDSHFHIGIYRPLAEDARSETASALVGGVTTVLSYFRTGS
                     HYLNKTGPYREIYPEVLRMTAGNAHTDYGYHLAIMTDEQVKEIPALVREHGVGSFKFY
                     MFYIGLNLAADSTAASAYTMADHYDLGHLYALMRGVAAAAREQTAGRVSLSIHCETAD
                     IIRACVEEQKASGANDLAAWSKARPPFSEAMAIAQATLLASRAGCAVNVLHISSAEAL
                     EAARLAGQRYPDLDLRLETTLHHLALTYDTAGDTHGKVNPPIRGADDVEAMWQAVRDG
                     RVGTVASDHAACPDEMKKDDLWSSQPGFGGTALLYPVLISEGFHKRKLPLARIADLAA
                     GEPARRFGLAPRKGAIAPGADADLAIVDPERTVTVAPELLQSAQPYTPFSGLKVRGWV
                     THTVLRGRVALEDGRVAGAPAGEYVRRPVPRAS"
BASE COUNT          337 a          926 c          984 g          385 t
ORIGIN      
        1 gacctccgcc tcgacgtggc ccatgcggcc ggggtagttg cggttcgtgc tggagatgca
       61 gcgctcgccc ttgccgagga tgccgtggcc cagcccggcg cacgcgccgc agccggtggg
      121 caggagcgtg ccccccgcct cgaggatggt cgcgagcacg cccgattccg ccgcgcgccg
      181 cgtgacgtcc ttcgacgcgg gcgcgatgag gaaccggatg ccgtccgcga cgtgccgccc
      241 cttcaccacg cgcgcgacgg cggcgaggtc ggtgtacttc gcgccggtgc aggcgccgat
      301 gtaggcctgg acgatcttct cgccgcgcgc gtcggtcgcg ggcgcgacgt tgtcgggcga
      361 tcccgggtac gcgacgagcg gcacgagctc gtcggcgcgg ttgcgcacca cgcgcgcgta
      421 ggtcgcgtcg gggtcgggat acgcggcgtc ctcgcggccc gcgccgccca cgaggtcgaa
      481 cacgagctcg tccggcggga tgatgccggt cttcgcgccg tgctcgatgg ccatgttggt
      541 gaggacgatc cgctcgtcga agggcagcgc gtagacggcc tcgccgccgt actcgagcgc
      601 gtggtacgtg gcgccgtcgt cgccgatctc gccgagcacc ttcagcacca tgtccttggc
      661 catgcacccc tcggcgagcc ggccggtcca ctcgatgcgc gtcgtcacgg ggaccttgag
      721 ccagatctcg cccgtcacga ggacgccgag catctcggtg acgcccaccg ggacggcgaa
      781 cgcgcccatc acgccggcgg tggtgctgtg cgagtcggcg ccgacgaaca gcatccccgg
      841 ccgcacgtag ccgcgctcga gcgggacgat gtggcagatg ccctcggcct cgtggtactt
      901 cgcgatgccg cggtcgtgcg cgaactcgcg cgtgacgcgc gcgatctcca cctcggtctg
      961 gtcgttcgcg ggcacgaagt ggtccgtcgc gatgacgagc ttgctcggat cccagatctg
     1021 cgcgccgagg cgctccagca tcggcccaag gcgccgcggt cccgacgagt cgtggaccct
     1081 cgcgaggtcg accccgcacc acacgatctc gccgggggtg acgctctcgc gcccgctctt
     1141 gcgcgcgagg atcttctcgg cgacggtctt tcccacggct acgacgcccg cggcacggga
     1201 cggcgcacgt actcgccggc tggcgcgccc gcgacgcgac cgtcctcgag cgcgacgcgg
     1261 ccgcgcagca cggtgtgcgt gacccagccg cgcaccttga gcccgctgaa gggtgtgtac
     1321 ggctgcgcgg actggagcag ctcgggcgcg acggtgaccg tgcgctcggg atcgacgatc
     1381 gcgaggtccg cgtccgcgcc cggcgcgatc gcgcccttgc gcggcgcgag gccgaagcgg
     1441 cgcgcgggct cgccggcggc gaggtcggcg atgcgcgcga gcggcagctt ccgcttgtgg
     1501 aagccctcgg agatgagcac cggatagagg agcgcggtac cgccgaagcc cggctgcgac
     1561 gaccagaggt cgtccttctt catctcgtcg ggacacgcgg cgtggtcgct cgcgacggtg
     1621 ccgacgcgcc cgtcgcgcac ggcctgccac atcgcctcca cgtcgtcggc gccgcggatc
     1681 ggcggattca ccttgccgtg cgtgtcgccg gcggtgtcgt acgtgagcgc gaggtggtgg
     1741 agcgtggtct ccagtcgaag atcgaggtcc gggtagcgct ggccagcgag ccgcgcggcc
     1801 tcgagcgcct ccgcgctcga gatgtggagg acgttcacgg cgcagccggc gcgcgacgcg
     1861 agcagcgtgg cctgcgcgat ggccatcgcc tcggagaacg gcggccgcgc cttcgaccac
     1921 gccgcgaggt cgttcgcgcc gctcgccttc tgctcctcga cgcaggcgcg gatgatgtcg
     1981 gcggtctcgc agtggatcga gagcgacacg cggccggcgg tctgctcgcg cgcggccgcg
     2041 gcgacgcccc gcatgagcgc gtagagatgg ccgaggtcgt agtggtcggc catcgtgtac
     2101 gccgacgcgg ccgtggagtc ggccgcgaga ttgaggccga tgtagaacat gtagaacttg
     2161 aaggagccga cgccgtgctc gcgcacgagc gcggggatct ccttcacctg ctcgtcggtc
     2221 atgatcgcga ggtggtagcc gtagtcggtg tgcgcgttcc ccgcggtcat gcgcaggacc
     2281 tcgggataga tctcgcggta cgggccggtc ttgttcaggt agtggctgcc cgtccggaag
     2341 taggagagca ccgtggtgac gccgcccacc agcgccgacg ccgtctcgga gcgggcgtcc
     2401 tcggcgagcg ggcggtagat gccgatgtgg aagtgcgagt cgatcgcgcc ggggagcacg
     2461 agcttcccgc gcgcgtcgat gacgcgctcg gcggccgtga ccggcaggtc gtcgccgatc
     2521 gcgacgatca cgccgtcgcg cgcggccacg tcggcgcgca ccgggccgac gtccggcagc
     2581 accgcggtgc cgccccgaac caggaggtcg tagcgctcgg tcacggcttc tc
//
LOCUS       DASTZR010000230         2799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_35820, whole genome shotgun sequence.
ACCESSION   DASTZR010000230 DASTZR010000000
VERSION     DASTZR010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35820"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /gene="leuC"
                     /locus_tag="VFV20_05140"
     CDS             complement(<1..1220)
                     /gene="leuC"
                     /locus_tag="VFV20_05140"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEU5287767.1"
                     /translation="MTARTLSEKVWDRHVVRRALGEPDLLYIDLHLVHEVTSPQAFDG
                     LRLAKRGVRRPELTLATMDHNVPTSAATKADDTSRAQMDAMTRNAREFGIRLYPIGAP
                     EQGIVHVISPQLGMTLPGMTIVCGDSHTSTHGAFGAIAFGIGTSEVEHVLATQTLWQQ
                     RPGTMAITVDGTLPPGVWAKDVILGIIARIGTSGGVGAIVEYRGSAIRALSMEGRMTV
                     CNMSIEAGARAGMVAPDDATFAYVEGRPHAPKGADLERALDEWRALTTDDGARFDREV
                     KIDASELRPYVTWGTNPAQSVTIDERVPDPASLPEAQREAASRALRYMDLRAGTAIRD
                     VAVDAVFLGSCTNARIEDLRVASEVVRGKRVKGGVRALVVPGSTAVKAQAEREGLDRI
                     FRDAGFEWRDAGCS"
     gene            complement(1217..2452)
                     /gene="ilvA"
                     /locus_tag="VFV20_05145"
     CDS             complement(1217..2452)
                     /gene="ilvA"
                     /locus_tag="VFV20_05145"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009487344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEU5287768.1"
                     /translation="MTLTARPLDTRAVTDAAERIAPHVRHTPTEQSPALSALAGRDVF
                     LKLENLQHTGAFKIRGALNAILQLDERARAKGVVTASAGNHGQGVAFAARLVGVPACV
                     VLPSGVPLAKLPATQRAGAETVLADGGYDGAHESAIALARERGMTYVHAFDDDDVIAG
                     QGTLALELMADADVDTLVVPVGGGGLIAGIALAVEARTPRPRIIGVQSENAPAFAQSF
                     GLGHAVERPAATIADGIAVRRPADRTLAVVRRLVDDVITVSDEAIARAIVVLLEHHKL
                     LAEGAGATALAAVLEANGRVGGKRVAAVISGGNIDPNLLGKVLQQGLASAGRYLAFRT
                     WLDDRPGMLHELTGILAAERINILHVGIHRLGPYAALGRVGLDVIVDTRDRAHADAIL
                     ALIRARGFSAEELLDTMPS"
     gene            complement(2493..>2799)
                     /locus_tag="VFV20_05150"
     CDS             complement(2493..>2799)
                     /locus_tag="VFV20_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5287769.1"
                     /translation="LYREKQERARAALAREFAGTGVSWNDPEGGFYFWVKLPHGMDAR
                     ALLDVALEEGVAFVPGQAFTVARDHRSALRFSISAPTPDRIDEGVRRLRRAFDRMQA"
BASE COUNT          369 a         1031 c         1011 g          388 t
ORIGIN      
        1 atcgagcagc ccgcgtcgcg ccactcgaac cccgcgtcgc ggaagatgcg gtcgagcccc
       61 tcgcgctcgg cctgcgcctt cacggccgtc gacccgggca cgacgagcgc gcgcacgccg
      121 cccttcacgc gcttcccgcg cacgacctcg ctcgcgacgc gtagatcctc gatgcgcgcg
      181 ttcgtgcacg agccgaggaa caccgcgtcg accgcgacgt cacggatcgc cgtgcccgcg
      241 cgcagatcca tgtaacggag cgcccgcgag gcggcctcgc gctgcgcctc cggcagcgac
      301 gcgggatccg ggacgcgctc gtcgatggtg acgctctgcg cggggttcgt gccccaggtg
      361 acgtacggcc gcagctcgct cgcgtcgatc ttcacctcgc ggtcgaaccg cgcgccgtcg
      421 tcggtggtga gcgcgcgcca ctcgtcgagc gcgcgctcga ggtccgcgcc cttcggcgcg
      481 tgcgggcgcc cctcgacgta cgcgaaggtc gcgtcgtccg gcgcgaccat gcccgcgcgc
      541 gcgcccgcct cgatcgacat gttgcagacg gtcatgcgcc cttccatcga gagcgcgcgg
      601 atcgccgacc cgcggtactc gacgatcgcg cccacgccgc cgctcgtccc gatgcgcgcg
      661 atgatcccga ggatcacgtc cttcgcccac acaccgggcg gcagcgtccc gtcgaccgtg
      721 atcgccatcg tccccggccg ctgctgccac agcgtctgcg tcgcgaggac gtgctcgacc
      781 tcgctcgtcc cgatgccgaa cgcgatcgcg ccgaacgcgc cgtgcgtgga ggtgtggctg
      841 tcaccgcaca cgatcgtcat ccccggaagg gtcatgccga gctgcggcga gatgacgtgc
      901 acgatcccct gctccggcgc gccgatcggg tagaggcgga taccgaactc gcgcgcgttc
      961 cgtgtcatcg catccatctg cgcgcgcgag gtgtcgtcgg ccttcgtcgc cgcgctcgtc
     1021 ggcacgttgt ggtccatcgt cgcgagggtg agctccggac ggcgcacgcc ccgcttcgcg
     1081 aggcgcagtc cgtcgaacgc ctgcggtgac gtgacctcgt gcaccaggtg caggtcgatg
     1141 tagagcaggt ccggctcgcc cagagcgcga cgcacgacgt ggcggtccca gaccttctcg
     1201 gagagcgtgc gtgcagtcat gacggcatgg tgtcgaggag ctcctcggcc gagaagccgc
     1261 gtgcgcggat gagcgcgagg atcgcgtccg cgtgcgcgcg gtctcgcgtg tcgacgatga
     1321 cgtcgagacc gacgcggccg agggcggcgt acgggccgag gcggtggatg ccgacgtgca
     1381 ggatgttgat gcgctccgcc gcgaggatgc cggtgagctc gtgcagcatc cccggccggt
     1441 cgtcgagcca ggtgcggaac gcgaggtagc gtccggccga cgcgaggccc tgctgcagca
     1501 ccttgccgag caggttcggg tcgatgttgc cgccgctgat gaccgcggcg acgcgcttcc
     1561 cgccgacgcg gccgttcgcc tccaggaccg cggcgagcgc ggtcgcgccg gcgccctcgg
     1621 cgagcagctt gtgatgctcg agcagcacga cgatcgcgcg cgcgatcgcc tcgtcggaga
     1681 cggtgatgac gtcgtccacg aggcggcgca cgaccgcgag cgtgcggtcc gcgggacggc
     1741 ggaccgcgat gccgtccgcg atcgtcgcgg ccggccgctc caccgcgtgg ccgagcccga
     1801 acgactgcgc gaacgccggc gcgttctcgg actgcacgcc gatgatgcgc ggccgcggcg
     1861 tgcgcgcctc gaccgcgagg gcgatgcccg cgatgagccc gccgccaccg accgggacca
     1921 cgagcgtgtc gacgtcggcg tccgccatga gctcgagcgc gagggtgccc tgtcccgcga
     1981 tgacgtcgtc gtcgtcgaac gcgtgcacgt acgtcatgcc tcgctcgcgc gcgagcgcga
     2041 tcgccgactc gtgcgcgccg tcgtacccgc cgtccgcgag cacggtctcc gcgccggcgc
     2101 gctgtgtcgc ggggagcttg gcgagcggca cgccggaagg gagcaccacg cacgcgggca
     2161 cgccgaccag acgcgcggcg aacgcgacgc cctgcccgtg gttgccggcc gacgccgtga
     2221 cgacgccctt cgcccgcgcc cgttcgtcga gctgcaggat cgcgttcagc gcaccgcgga
     2281 tcttgaacgc gccggtgtgc tgcaggttct cgagcttgag gaagacatcg cggcccgcga
     2341 gcgcggagag cgcgggcgac tgctcggtgg gcgtgtgacg cacgtggggc gcgatgcgct
     2401 cggccgcgtc cgtgaccgcg cgcgtgtcga gcggacgcgc ggtcagcgtc acggtcgacg
     2461 cccggtccag ctgttgtcgc gacgggcgta cgtcacgcct gcatccgatc gaacgcgcgg
     2521 cgcaagcgtc gcacgccctc gtcgatgcgg tcgggcgtcg gcgcgctgat ggagaaacga
     2581 agcgcgctgc ggtgatcgcg cgcgaccgtg aaggcctggc ccgggacgaa cgcgacgccc
     2641 tcctcgagcg cgacgtcgag cagcgcacgc gcgtccatgc cgtgcggcag cttcacccag
     2701 aagtagaagc cgccctcggg atcgttccag ctcacgcctg tacccgcgaa ctcgcgcgcg
     2761 agcgcggcgc gcgcccgctc ctgcttctcg cgatagaga
//
LOCUS       DASTZR010000231         3605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_953068, whole genome shotgun sequence.
ACCESSION   DASTZR010000231 DASTZR010000000
VERSION     DASTZR010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953068"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..1379
                     /locus_tag="VFV20_05155"
     CDS             69..1379
                     /locus_tag="VFV20_05155"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287770.1"
                     /translation="MPSDTQTTSRTPRAGRTPTPSQTPTTSRTGHPAPRHAIVPIVLI
                     ALAATPALLTRIGATTPSVELGTFYFGISVIAAAFAMSWASEAAEHDVPRALSLLVVA
                     LIAVLPEYAVDIVFAWKAGQDPSFAPYAAANMTGSNRLLLGVGWSTVALLAFIAERRR
                     IITLDRGGGVAVVFLAIATLYSFTLPFRGAITLLDTLLLAILFFTYAAWAARSGMEEP
                     ELVGPAAAIGALSTRGRRAVVLALFAFAGVVIGMAAEPFADGLVRTGRSLGIDEFLLV
                     QWLAPLASESPEFIVAALLTLRGKAVVAFAILLSAKINQWTLLVGSLALSFSIGAGTP
                     GALPLDARQSAEVLLTASQSFFGLAVLASLTFGLGEAALLAGLFFAQFIAGGILRAVL
                     RSSGAAEIELYAFSVLYLVLAAGALYRARHALPRPWRARRRAPG"
     gene            complement(1411..1587)
                     /locus_tag="VFV20_05160"
     CDS             complement(1411..1587)
                     /locus_tag="VFV20_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287771.1"
                     /translation="MNNDDSPAIDTGETETETAVRTWLAEQADLLTLEEGFDRAQLAG
                     PYICLGPRERGEAA"
     gene            complement(1596..2720)
                     /locus_tag="VFV20_05165"
     CDS             complement(1596..2720)
                     /locus_tag="VFV20_05165"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEU5287772.1"
                     /translation="MNLAPTPLLPDGYQVPASRWEDPATRMRRLLDTEPFVFGPGVYD
                     PHGAELCMYYGFKAVYFSGYSFAIGHLGTTDMDLYTGPEIADGARRTIAALRKFQLTM
                     ALGEPDKGVAPRHLEIPPLLVDMDGGYGNLFNVQRTAELYVNAGVAAAHIEDQVLPKR
                     CGHIAGKALISADEMIGKLRMMRAVADDLGRPDFVVVARTDGVSAVDAPEKTRGIELA
                     IDRALRYLDSGVPDLVWCEFPTSDRAPTERFAQEVSRRFPNARLAYNWSSSFKWFNDP
                     SPISFAELGELGYKFLFITLAAQHAYGYGFAKLLQGLRDEEQQAYVELQKKEWQVGDD
                     IPTRSHHLFTGVPYHQTMGSVMGASRLGTEFDEQLGDKAV"
     gene            complement(2717..2866)
                     /locus_tag="VFV20_05170"
     CDS             complement(2717..2866)
                     /locus_tag="VFV20_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287773.1"
                     /translation="MDTQKATTEEVDMTEIEERIEDQRDQARLLQRQLDCECPDAECP
                     VEHES"
     gene            2949..>3605
                     /locus_tag="VFV20_05175"
     CDS             2949..>3605
                     /locus_tag="VFV20_05175"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU5287774.1"
                     /translation="MPQDPMLFAHLESFVEVARQGNVSRAADSLFLTQPAITARLKSL
                     EADLGVELFVRTHRGMKLTDAGRAFLPYAERTLATVDEGRQLMTSLREGTTGALVIAA
                     APAVSTYVLPGVLRAFRTHHPDVRLGVRTGHTEEVVELVLRGEADLGVGRPVQNPEVE
                     LIPVFEDEMLLVVNARHHFASRGKVSLSELAGERLVLFDRTSSYHELTSSLFRQAGVV
                     P"
BASE COUNT          471 a         1288 c         1245 g          601 t
ORIGIN      
        1 atggatccgc acgaccccgc cggagcagcg gaacagcctc gtcgccgtca tcgaggacct
       61 cggcctggat gccgagcgat acgcagacga cctcgaggac gccgagggct gggaggacgc
      121 cgacgccgag ccagaccccg acgacgagta ggaccggcca ccccgcaccg cgccacgcca
      181 tcgtccccat cgtcctcatc gccctcgcgg cgacgcccgc gctgctcacg cgcatcggcg
      241 cgaccacccc ctcggtcgag ctcggcacgt tctacttcgg gatcagcgtc atcgcggccg
      301 cattcgcgat gtcgtgggcg tcggaggccg cggagcacga cgtaccgcgt gcactctcgc
      361 tcctcgtcgt cgcgctcatc gcggtgctgc cggagtacgc ggtggacatc gtgttcgcgt
      421 ggaaggccgg gcaggatccg tccttcgcgc cctacgccgc ggcgaacatg acgggatcga
      481 accggctgct cctcggcgtg ggctggtcga cggtggcgct gctcgccttc atcgccgagc
      541 gtcgccggat catcaccctc gatcggggcg gcggtgtggc ggtggtcttc ctcgcgatcg
      601 ccacgctcta ctcgttcacg ctccccttcc gtggcgcgat cacgctcctc gacacgctcc
      661 tgctcgcgat cctcttcttc acctacgccg cgtgggccgc gcgcagcggc atggaggagc
      721 ccgagctcgt cgggccggcc gccgcgatcg gagcgctctc gacgcgcggg cggcgggcgg
      781 tggtgctcgc gctcttcgcg ttcgccggcg tcgtcatcgg catggccgcg gagcccttcg
      841 cggacggcct cgtccggacg gggcgctcac tgggcatcga cgagttcctg ctcgtgcagt
      901 ggctcgcgcc gctcgcgagc gaatcgccgg aattcatcgt cgcggcgctg ctcacgctcc
      961 gcggcaaggc ggtggtcgcg ttcgcgatcc tgctctccgc gaagatcaac cagtggacgc
     1021 tcctggtcgg cagcctcgcg ctgtcgttct cgatcggcgc gggcacgccg ggcgcgctgc
     1081 cgctcgacgc gcgccaatcg gcggaggtgc tgctcaccgc gtcgcagagc ttcttcggtc
     1141 tcgccgtgct cgcgagcctc acgttcggcc tcggcgaggc cgcgctgctg gcgggcctct
     1201 tcttcgcgca gttcatcgcg ggcggcatcc tgcgggcggt actgcgctcg agcggcgcag
     1261 cggagatcga gctctacgcc ttcagcgtcc tgtatctggt gctcgcggcg ggggccctgt
     1321 accgcgcccg ccacgcgctg ccgcgcccgt ggcgcgcacg acgacgcgcg ccggggtgag
     1381 cggtggcgcg ccgcggacgg acagcgccgc ttaggcggct tcgccccgtt cgcgcggccc
     1441 gaggcagatg tagggacccg cgagctgcgc gcggtcgaat ccctcttcga gcgtgagcag
     1501 gtccgcctgc tcggccagcc acgttcggac cgcggtctcc gtctcggtct cgcccgtgtc
     1561 gatcgccggg ctgtcgtcgt tgttcatgga tcctcctaga cggccttgtc gccgagctgc
     1621 tcgtcgaact ccgtgccgag gcgcgaagcg cccatgacgg agcccatcgt ctggtggtac
     1681 gggacgccgg tgaacaggtg gtggctgcgc gtggggatgt cgtcgcccac ctgccattcc
     1741 ttcttctgca gctcgacgta ggcctgctgc tcctcgtcgc gcagaccctg gagcagcttc
     1801 gcgaagccgt agccgtacgc gtgctgcgcc gcgagggtga tgaagaggaa cttgtagccg
     1861 agctcgccga gctccgcgaa gctgatcggg ctcgggtcgt tgaaccactt gaacgacgac
     1921 gaccagttgt acgcgaggcg cgcattcggg aagcggcggc tcacctcctg cgcgaagcgc
     1981 tccgtcggag cgcggtcgga cgtcgggaac tcgcaccaga ccaggtccgg cacgccggag
     2041 tcgaggtacc ggagggcccg gtcgatcgcg agctcgatgc cgcgggtctt ttcgggggcg
     2101 tccaccgcgg acacgccgtc ggtgcgcgcg acgacgacga agtcgggacg gccgaggtca
     2161 tccgcgaccg cacgcatcat gcgcagcttc ccgatcatct cgtcggcgct gatgagcgcc
     2221 ttgccggcga tgtgcccgca gcgcttcggg aggacctggt cctcgatgtg cgccgcggcg
     2281 acgcccgcgt tcacgtagag ctccgcggtc cgctggacgt tgaacaggtt gccgtagccg
     2341 ccgtccatgt cgacgaggag cggcgggatc tcgaggtgcc tcggcgcgac gcccttgtcg
     2401 ggctcgccga gcgccatcgt gagctggaac ttgcggagcg ccgcgatcgt gcggcgggcg
     2461 ccgtcggcga tctcgggccc ggtgtacaga tccatgtcgg tcgtgccgag gtgcccgatc
     2521 gcgaacgaat agccgctgaa gtacacggcc ttgaagccgt agtacatgca gagctcggcg
     2581 ccatgcgggt cgtacacgcc gggcccgaac acgaacggct cggtgtcgag cagcctgcgc
     2641 atgcgcgtgg ccggatcctc ccagcgcgat gccgggacct gatagccgtc cggcaggagc
     2701 ggtgtgggcg cgagattcac gattcgtgct ccaccgggca ctccgcgtcc gggcactcgc
     2761 agtcgagctg tcgctggagc aagcgcgcct ggtcccgctg gtcctcgatg cgctcctcga
     2821 tctcggtcat gtcgacctcc tcggtggtcg ccttctgggt atccatgcgc gcgctcagcg
     2881 cactctccgt gccacagggc ctatcagtcc aattgcttgt tctgatgcgt gctataaagc
     2941 agattgatat gccccaggac cccatgctct tcgcccatct ggagtcgttc gtcgaggtgg
     3001 ctcggcaggg caacgtgagc cgcgccgcgg actcgttgtt cctcacgcag ccggccatca
     3061 ccgcccggct caaatcactg gaggccgacc tgggcgtcga gctgttcgtc cgcacgcacc
     3121 gcgggatgaa gctcaccgac gcgggccgcg cgttcctccc ctacgccgag cgcaccctcg
     3181 ccaccgtcga cgagggccgg cagctcatga cgagcctgcg cgagggcacg accggcgcgc
     3241 tggtgatcgc ggcggcaccg gcggtgagca cctacgtgct cccgggcgtg ctacgcgcgt
     3301 tccgcacgca ccatcccgac gtccgtctgg gggtccgcac ggggcacacc gaggaggtcg
     3361 tcgagctggt gctgcgcggc gaggccgacc ttggcgtcgg ccggcccgtc cagaaccccg
     3421 aggtcgagct catcccggtg ttcgaggacg agatgctgct cgtcgtgaac gcgcggcacc
     3481 acttcgcgtc gcgtggcaag gtcagcctgt cggagctcgc aggcgagcgc ctcgtgctct
     3541 tcgaccgcac gtccagctac cacgagctca cgtcgagcct cttccgccag gccggcgtcg
     3601 tcccc
//
LOCUS       DASTZR010000232         2479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1211461, whole genome shotgun sequence.
ACCESSION   DASTZR010000232 DASTZR010000000
VERSION     DASTZR010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211461"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VFV20_05180"
     CDS             complement(<1..688)
                     /locus_tag="VFV20_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEU5287775.1"
                     /translation="MDVIGQLANGFSIAITPQNLGLAFAGVFIGTVIGILPGIGPGAG
                     VALLLPVTFGLPPVSGLIMLAGIYYGAMYGGTLTSVLINTPGESSSVMTTIDGYQLAR
                     QGRAGAALAIAAIGSFIAGTLGVVALMLAAPPIADQAVKFGPPEYFMLAVLGLLTLAA
                     LGGTSTLKSLLMAVTGLAIATVGLDPMIGEQRFTFGILELSDGVEFLPVAVGLFGIAE
                     VIANIETRLRL"
     gene            complement(689..1132)
                     /locus_tag="VFV20_05185"
     CDS             complement(689..1132)
                     /locus_tag="VFV20_05185"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEU5287776.1"
                     /translation="MTRADTVIGAGLAVVFLAFLFAATAHTYQDEFSPGPGFAPFWFG
                     AVGAALSLFVAVRGIRAAPAPTFTRAGVLRAAITVAGLIVALALAEAVGFIVTMTVYL
                     LVVMLAVERMRPVPALATAFGTMLLVYVVFARFLAVPLPKGPLGF"
     gene            complement(1173..2213)
                     /locus_tag="VFV20_05190"
     CDS             complement(1173..2213)
                     /locus_tag="VFV20_05190"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU5287777.1"
                     /translation="MRITRTVVTLAVAAGLVAGACGGGGATASPTPAPKTSAPVAATS
                     TPAPTWNPTKDVELVVQAAAGGGSDIFARKIADVLTKEKLSAKPINVVNKPGGSGAVA
                     YAYIKQKQADPHFIATVTLSYLTTPLSNTSAGYTYSELSNIAILAVDDFVAVVKDDAS
                     YKTLKDIVDAAKSKGPKQIKVGGTQVGSSDSIIPALIEQATGVQFNYITFQSGGEVNA
                     ALLGGTVDFAIANPAEALSLVQGKKLRAVASFSPEPLKGWTATTAKSQGIDVIWEQFR
                     GITAPGGLKEIEQKYWADALEKMTKTADWAKYLDDNTLRPVFKKGADAKKYLDDQNTM
                     LKTVLTKLGVIK"
     gene            complement(2210..>2479)
                     /locus_tag="VFV20_05195"
     CDS             complement(2210..>2479)
                     /locus_tag="VFV20_05195"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEU5287778.1"
                     /translation="DAARSPQLVRLIDSLGTRLRRARVASLSLADRPARALDEHRALA
                     AAVIARDPAKARVLAAEHAERGRRAATNWYLEHHRVTGTNGGGRS"
BASE COUNT          394 a          822 c          879 g          384 t
ORIGIN      
        1 ccaggcgcag ccgcgtctcg atgttcgcga tcacctcggc gatgccgaag agtccgaccg
       61 cgacgggcag gaactcgacg ccgtccgaga gctcgaggat cccgaacgtg aaccgctgct
      121 cgccgatcat gggatcgagg cccacggtcg cgatggcgag accggtgacg gccatgagca
      181 gtgacttcag cgtcgaggtg ccgccaaggg cggcgagggt gaggaggccc agcaccgcga
      241 gcatgaagta ctccggcgga ccgaacttca ctgcctgatc ggcgatcggc ggggccgcaa
      301 gcatgagcgc gacgacgccg agcgtgccgg cgatgaagga tccgatcgcg gcgatcgcga
      361 gcgcggcgcc cgcgcgtccc tgtcgcgcga gctggtagcc gtcgatcgtc gtcatcacgc
      421 tcgacgactc gccgggagtg ttgatgagga cggaggtgag cgtgccgccg tacatcgccc
      481 cgtagtagat gccggcgagc atgatcagcc cggagacggg cggcagcccg aacgtcaccg
      541 gcagcagcag cgcgacgccg gcccccgggc cgatgcccgg caggatgccg atgacggtgc
      601 cgatgaagac gccggcgaac gcgaggccga ggttctgtgg cgtgatcgcg atggagaacc
      661 cgttcgcgag ctgcccgatg acgtccatct agaagcccag cggacccttc gggagcggga
      721 ccgcgaggaa gcgcgcgaac acgacgtaca cgagcaacat cgtcccgaac gcggtcgcca
      781 gcgcggggac gggacgcatc cgctccacgg cgagcatcac gaccaggaga tacacggtca
      841 tcgtcacgat gaagccgacc gcctcggcga gagcgagtgc gacgatcagg ccggcgacgg
      901 tgatggcggc ccgcagcacg ccggcacgcg tgaacgtcgg agcgggcgcg gcgcggatgc
      961 cgcggacggc gacgaacagg gacagcgccg ctccgacggc gccgaaccag aagggcgcga
     1021 atccgggtcc gggcgagaac tcgtcctgat aggtgtgtgc ggtcgcggcg aagaggaacg
     1081 cgaggaatac gacggccagc ccggcgccga tgacggtatc ggcgcgcgtc atgcgcgcgt
     1141 caccgcggcg ccgggctctg ccgtcgcgaa cgtcacttga tgacgcccag cttggtgagg
     1201 accgtcttca gcatcgtgtt ctggtcgtcg agatacttct tggcatccgc gcccttcttg
     1261 aacaccgggc gcagcgtgtt gtcgtcgagg tacttcgccc agtcggcggt cttcgtcatc
     1321 ttctcgagcg catcagccca gtacttctgc tcgatctcct tgaggccgcc gggagcggtg
     1381 atgccacgga actgctccca gatgacgtcg atgccctgag acttcgcggt ggtcgccgtc
     1441 cagcccttga gcggctcggg tgagaagctc gcgacggcgc ggagcttctt gccctggacg
     1501 agcgagagcg cctcggcggg gttcgcgatc gcgaagtcga ccgtgccgcc gagcagcgcg
     1561 gcgttgacct cgccgccgct ctggaaggtg atgtagttga actgcactcc ggtggcctgc
     1621 tcgatgaggg cggggatgat cgagtcggac gaaccgacct gcgtcccgcc gaccttgatc
     1681 tgcttcggcc ccttcgactt cgcggcgtcc acgatgtcct tcagcgtctt gtatgacgcg
     1741 tcgtccttga ccacggccac gaagtcatcg accgccagga tggcgatgtt cgagagctcc
     1801 gagtaggtgt atcccgcgct ggtgttcgag agcggggtcg tcaggtagct gagcgtcacc
     1861 gtcgcgatga agtgcgggtc ggcctgcttc tgcttgatgt acgcatacgc gaccgcgccg
     1921 ctgccgcccg gcttgttcac gacgttgatg ggcttcgccg acagcttctc cttcgtcagc
     1981 acgtcggcga tcttgcgcgc gaagatgtcg ctgccaccgc cggccgccgc ctgaacgacg
     2041 agctcgacgt ccttcgtcgg gttccaggtg ggcgccggcg tgctcgttgc cgccaccggg
     2101 gcactggtct tgggtgcggg tgtcgggctc gccgtcgctc cgcccccgcc gcacgcacct
     2161 gcgacgagac cggcggcgac cgccagggtc acgacggttc gggtgatcct catgaacgtc
     2221 ctcctccatt tgtgcccgtg acgcggtgat gctccagata ccaattcgtt gccgcacgcc
     2281 gtccgcgctc cgcatgttcg gccgccagga cgcgggcctt cgccgggtcg cgcgcgatga
     2341 ccgcggcagc gagcgcgcgg tgctcgtcga gcgcccgcgc cggacggtcc gcgagcgaca
     2401 ggctcgcgac gcgagcgcgc cgaagacgcg tacccagact gtcgatgagg cgcaccagct
     2461 gcgggctgcg cgcggcgtc
//
LOCUS       DASTZR010000233         2078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_744597, whole genome shotgun sequence.
ACCESSION   DASTZR010000233 DASTZR010000000
VERSION     DASTZR010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744597"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..819
                     /locus_tag="VFV20_05200"
     CDS             238..819
                     /locus_tag="VFV20_05200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5287779.1"
                     /translation="MTIINIKVDALPLEQIPVPDVIERARLGDRGAFAELYDTHCDAV
                     YRYVLYRVREPSDAEDLTSEVFTRAFANIHRYRWQGKSFLAWLYTIARNAVTDRRRRE
                     RPTVDLDNAYGIAEDGPTAHDRAVRGEQVDALRGAVKHLTTEQQEVLLLRFVENMSSR
                     QVAKVLGKNEGAIRALQFRALGRLRKLLTAEVA"
     gene            871..>2078
                     /locus_tag="VFV20_05205"
     CDS             871..>2078
                     /locus_tag="VFV20_05205"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEU5287780.1"
                     /translation="MLGLRRLPFIMALAAVAVACSILPAELQRELLPSQTQYKTAEAA
                     FQVLIDKHVDRPTSTKLIPGALDGVTNYLKNQNIDANPVVDRPALTGSEWSDFAKLSA
                     SMDATLARYPAAKKDLLERAAVDGMARSMNECHTYYLDPDRARTFNRPPAPVSGIGVT
                     INQPDPNSPIEIIDVIPGTPAERAGVRKGDRIMKVNGEDVAHLTTSEVADRVRGPAGS
                     SVTIVFDRNGSPVQLTITRAQFTTPLTESSVQANDVAYVRIKQLISNVADDAAAALRR
                     MSSARGVILDLRDDPGGELSVAVDVGSLFVKTGPLVFQTGRDGQKTPIDVNPRRYLGT
                     SVPVVVLVNKNSASGSEIIAAGVRSSGAGTVMGTQTAGCVGSGQPRDLPDGGLLLVTL
                     TKMQDAKTGA"
BASE COUNT          349 a          716 c          697 g          316 t
ORIGIN      
        1 tctaggtcgc tcgccgcgcg cagcgcgcac gtgttgtgcg ctgacccatc catgcgacgg
       61 cacgcttgct cgcgctgaag gtgcgacggt cgtactagga ccctcggctg cacgtacggt
      121 ccgcgcgcgt ttcgtgacga catgtgacgg ttttggtacc gaagatgggt tggcgtgagg
      181 cgcatgcgcc gatatggtcc gtctcaacac gaggtcggga tagggagcac tcacgtcgtg
      241 acgatcatca acatcaaggt agacgcgctg ccactcgagc agatccccgt accggacgtc
      301 atcgagcgtg cgcgtctggg ggaccgcggc gcattcgccg agctgtacga cacgcactgc
      361 gatgccgtgt accggtacgt cctgtaccgc gtgcgcgagc cctcggacgc ggaggacctc
      421 accagcgagg tcttcacccg ggcgttcgcg aacattcacc gctaccgctg gcagggcaag
      481 tcattccttg cctggctgta caccatcgcc cgcaacgccg tcaccgatcg tcgccgccgc
      541 gagcggccga ccgtcgacct cgacaatgcc tatggcatcg ccgaggacgg cccgaccgcc
      601 catgaccgcg cggtgcgcgg cgagcaggtg gacgcgctgc gcggcgcggt gaagcacctg
      661 acgaccgagc agcaagaggt cctgctgctg cgcttcgtcg agaacatgtc gagccggcag
      721 gtcgcgaagg tgctgggcaa gaacgagggc gcgatccgcg cgctccagtt ccgcgccctc
      781 gggcgactgc gcaagctgct caccgcggaa gtcgcctagc tcacagcgcg gtccattccg
      841 cgttctcgcc ggcccgtaga ctggtcagta gtgctcgggc tgcggcggtt gccgttcatc
      901 atggccctcg ccgcggtcgc cgtggcgtgt tccatcctgc ccgcggagct gcagcgcgag
      961 ctgctcccct cgcagacgca gtacaagacg gccgaggcgg cgttccaggt cctcatcgac
     1021 aagcacgtcg accggcccac ctcgacgaag ctcatcccgg gtgcgctcga cggcgtcacc
     1081 aattacctga agaaccagaa catcgacgcg aacccggtcg tcgatcgacc cgcgctcacg
     1141 ggcagcgagt ggtcggactt cgccaagctc tccgcctcga tggatgcgac gctcgcgcgc
     1201 tatcccgcgg cgaagaagga cctgctcgag cgcgcggccg tcgacggcat ggcgcgatcg
     1261 atgaacgagt gtcacaccta ctacctcgat cctgaccgcg cgcgcacgtt caaccggccc
     1321 cccgcgccgg tctcgggcat cggggtcacg atcaaccagc cggatccgaa ctcaccgatc
     1381 gagatcatcg acgtcattcc cgggacgccc gccgagaggg ccggcgtccg caagggcgac
     1441 aggatcatga aggtgaacgg cgaggacgtg gcgcacctca cgacgtccga ggtggccgat
     1501 cgcgtgcgcg gcccggcggg ctcgtcggtg acgatcgtct tcgaccggaa tggcagcccg
     1561 gtgcagctca ccatcacgcg ggcccagttc acgacgccac tgaccgagtc gagcgtgcag
     1621 gcgaacgacg ttgcctacgt gcggatcaag cagctcatct cgaacgtcgc ggacgacgcc
     1681 gcggccgcgt tgcgcaggat gtcgtcggcc cgcggtgtca tcctcgacct gcgtgacgac
     1741 cccggcggcg aactgtccgt ggcggtggac gtgggcagcc tgttcgtgaa gacgggcccg
     1801 ctggtcttcc agaccggccg tgacggccag aagacgccga tcgacgtgaa cccgcgccgc
     1861 tacctcggca cgagcgtccc ggtcgtcgtg ctcgtgaaca agaattccgc gtcgggctcg
     1921 gagatcatcg ccgcgggcgt gcgctcgagc ggcgccggca cggtcatggg aacgcagacc
     1981 gccggatgcg tgggtagcgg acagccacgc gacctcccgg atggcggcct gctgctggtg
     2041 acgctcacga agatgcagga cgccaagacc ggcgcgga
//
LOCUS       DASTZR010000234         2100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1162075, whole genome shotgun sequence.
ACCESSION   DASTZR010000234 DASTZR010000000
VERSION     DASTZR010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162075"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VFV20_05210"
     CDS             complement(<1..233)
                     /locus_tag="VFV20_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEU5287781.1"
                     /translation="MTRRVVVTGLGMVTPLGLDVPSTWDALVHGRSGITRITRFDPAP
                     YETQIAGEVHGYEPTQYMDRKEIRRTDRFTHFAV"
     gene            complement(230..1252)
                     /locus_tag="VFV20_05215"
     CDS             complement(230..1252)
                     /locus_tag="VFV20_05215"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU5287782.1"
                     /translation="MSAFAFVFPGQGSQLVGMGRSVYESSPAARAVFDEADRVLGYPL
                     TKICFEGPAEQLNDTTVAQPAVLTTSIALFEAMVEAVSHRLGRRATDGSEPRGVMALP
                     EPMRPAFVAGHSLGEFTALVIAGVLPFSDALRLVATRGQLAATRGAQGSMAAIIGMSA
                     QDVEDVIASTGLAAGRVVVANDNGPNQVTIAGDAEGVTKVTEALASGGAKKVVPLRIS
                     APFHFPAMGKIGEELRAYMQTLRFREPVVPIVANVSGLPHPNVSSIPDALVRHLSNRV
                     EWLKSVRYMVERGAGTFVEFGGGPVVNGLVKRIADVSLVNITDRASVAKAIESLRAKA
                     SAVTQR"
     gene            1387..1977
                     /locus_tag="VFV20_05220"
     CDS             1387..1977
                     /locus_tag="VFV20_05220"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5287783.1"
                     /translation="MRRYVQRERAKRTARTATAIENAALREVARRGYPDLRIADVARR
                     AHVSSRTVYLHSASKERLVEQALRRRGAALISRVERWRPRGDTPQQIVDEMVALHERE
                     YRGESALLLETLVDGALPRGVAALLRDLDAVRLAVITRTMTDLGRRGALRIRTQDAIA
                     LAHALLSYPTWRTALTGPARRRAPRLVASALRSALL"
BASE COUNT          315 a          742 c          729 g          314 t
ORIGIN      
        1 acggcgaaat gcgtgaagcg atcggtccgg cggatctcct tgcggtccat gtactgcgtc
       61 ggctcgtatc cgtgcacctc gcccgcgatc tgcgtctcgt atggcgcggg atcgaagcgg
      121 gtgatgcgcg tgatgccgct acgtccgtgc acgagggcgt cccaggtgct ggggacgtcg
      181 aggccgagcg gggtgaccat gccgaggccg gtcacgacga cgcggcgcgt caccgctggg
      241 tcaccgcgct cgctttcgcg cgcagcgatt cgatcgcctt cgcgaccgac gccctgtcgg
      301 tgatgttcac caggctcacg tcggcgatgc gcttcacgag gccgttcacg accggaccgc
      361 caccgaactc gacgaacgtc ccggcgccgc gctcgaccat gtagcgcacg gacttcagcc
      421 actccacgcg gttcgagagg tgacgcacga gggcgtccgg gatcgacgac acgttcggat
      481 gcggcagacc gctcacgttc gcgacgatcg ggacgaccgg ctctctgaac cggagggtct
      541 gcatgtacgc gcggagctcc tcgccgatct tccccatcgc cgggaaatgg aacggcgccg
      601 agatgcgcag cggcacgacc ttcttcgcac cgcccgacgc gagagcctcg gtgaccttgg
      661 tgacgccctc cgcgtcgccg gcgatcgtga cctgattcgg cccgttgtcg ttcgccacga
      721 cgacgcgccc ggcggcgagc ccggtcgagg cgatcacgtc ctccacgtcc tgcgcgctca
      781 tgccgatgat cgcggccatc gatccctgtg cgccgcgcgt ggcggccagc tgcccgcggg
      841 tcgcgacgag gcggagcgcg tcggagaacg ggaggacgcc ggcgatgacc agcgcggtga
      901 actcgccgag cgagtggccg gccacgaatg ccggacgcat cggctcggga agcgccatca
      961 cgccccgcgg ctcgctgcca tcggtcgcac ggcggccgag gcgatgcgac acggcttcca
     1021 ccatcgcctc gaagagcgcg atgcttgtcg tgagcaccgc cggctgtgcg acggtcgtgt
     1081 cgttcagctg ctccgcgggg ccctcgaaac agatcttcgt gagcggatag ccgaggacgc
     1141 gatcggcctc gtcgaagaca gcacgagccg ccggtgagga ttcgtagacc gaacgaccca
     1201 tccctaccag ctgcgagccc tgtccgggaa acacgaaagc gaaggcgctc attgcggacc
     1261 atgagcgtag cagtccaggg ccgtcgtggt cgaggaccaa accgcattcg attacagtgc
     1321 tattgcaatg aatacacgac gtgcgcacca gccgagtggg cgcacgtcgc aggaggtgcg
     1381 atagcgatgc gcagatacgt gcagcgcgaa cgcgcgaagc gcaccgcccg gacggcgacg
     1441 gcgatcgaga atgccgcgct gcgtgaggtc gcgcgtcgtg gctatccgga tctgcgcatc
     1501 gccgacgtcg cgcgccgcgc gcacgtctcg tcgcgcaccg tgtacctgca ctcggcatcg
     1561 aaggagcggc tcgtcgagca ggcgctccgg cgccgcggtg ccgcgctcat ctcgcgggtc
     1621 gagcgttggc ggccccgcgg cgacacgccg cagcagatcg tcgacgagat ggtcgcgctc
     1681 catgagcgcg agtaccgcgg cgagtcggcc ctgctcctgg aaacgctcgt cgacggcgcg
     1741 ctcccacgcg gcgtcgccgc gctgctccgc gacctcgacg ccgtgcgcct cgcggtgatc
     1801 acgcgcacga tgaccgacct cggtcgcagg ggcgcgctcc gcatccgcac gcaggacgcg
     1861 atcgcgctcg cgcacgcgct gctctcctac ccgacgtggc gcaccgcgct caccggcccg
     1921 gcgcgtcgtc gcgccccgcg cctcgtggcg agcgccctgc ggagcgcgct gctgtgacat
     1981 tcgactcttt cccgctgccg cgcgcgggat gacagtctgt gcgtgctcgc gctgtggcgg
     2041 aactggatcg tcgcgaacgt cgtcggcgag atcgtcggct tcggcctcgc cggcgcggtg
//
LOCUS       DASTZR010000235         4405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1083547, whole genome shotgun sequence.
ACCESSION   DASTZR010000235 DASTZR010000000
VERSION     DASTZR010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083547"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VFV20_05225"
     CDS             <1..370
                     /locus_tag="VFV20_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287784.1"
                     /translation="ATGATGAGCPTGATGATGATGATGATGATGATGATGATGEATGK
                     PDQQGRVTVAQRTTLEANIQSIVDLAIKDMDDLVKSATTEAAAVLPAQHGNSGDNGKS
                     GDNPGNKPENAGKPTGSPKR"
     gene            496..1044
                     /locus_tag="VFV20_05230"
     CDS             496..1044
                     /locus_tag="VFV20_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287785.1"
                     /translation="MRILIATLALMLALGTTALAGGWAVTTLDTLPPDISAGQTYTIG
                     WTIRQHGVTPVDVERLGGTTELIATSPDGSRTVSFAGRRDGDTGHYAAKVTFPSEGTW
                     TWKVTQAPFQPQPLGTVVVSPALAGSDAARGVNAANTAASGAPDPISNPWLVLALLLA
                     SGGAAVLFGTRVAALTVRPRSA"
     gene            1050..1475
                     /locus_tag="VFV20_05235"
     CDS             1050..1475
                     /locus_tag="VFV20_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU5287786.1"
                     /translation="MRREVADVVGSGLLLAALVTATIGVMTDRTASASGSSPSTTVSL
                     TDASLVARGRSLFSAKGCVACHTKEQQAIVQVGPALTGLRDRAAERRPGLDAVAYVRE
                     SIRTPAAFIVPGYGNAAMPDLALRDEDIDAIAAYLLGTP"
     gene            complement(1478..1792)
                     /locus_tag="VFV20_05240"
     CDS             complement(1478..1792)
                     /locus_tag="VFV20_05240"
                     /inference="COORDINATES: protein motif:HMM:NF036395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase family protein"
                     /protein_id="HEU5287787.1"
                     /translation="MLAQFMKRGARMRGVTVRPSRFKGPRHTDALWYGTREVAHCHDG
                     QVDVRLTRQVVRELRDELRSDARIDLRGPGFDWILVRVRRAADVERALELLRHAVRVA
                     VR"
     gene            complement(1815..2336)
                     /locus_tag="VFV20_05245"
     CDS             complement(1815..2336)
                     /locus_tag="VFV20_05245"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEU5287788.1"
                     /translation="MTLVDVALLFLRVGAVSFGGGASVLADLDHELVDQRAAMTQQQF
                     LNAYAIGQATPGPGILFLVPLGFYAAGAPGAVVALVAFLVPPLLLQVVVASQWERLSR
                     SPWIRALDRTLVPLSVGLIGASLYSLGTPLLPLPTHVVGLVVAMLASTVLRLSPFVIV
                     VGAGLLGLLGVFS"
     gene            complement(2333..2854)
                     /locus_tag="VFV20_05250"
     CDS             complement(2333..2854)
                     /locus_tag="VFV20_05250"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEU5287789.1"
                     /translation="MRFGGVLGKFLWVGLTAFGAARWVNLENAFVRTGAIREEDFMRD
                     LAIAQTLPGPGFVNLTALCGMRLGGPRTAFLAIVLVLLPGLVAVTAVLAFLSMSEPWV
                     ARLFQGILVGAVGVLAASFVRMTRRLQRSFDALLTAATLILIVAGTPMVAAVLIVGGI
                     GVLRYRWSRLTLP"
     gene            2877..3164
                     /locus_tag="VFV20_05255"
     CDS             2877..3164
                     /locus_tag="VFV20_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI-like protein"
                     /protein_id="HEU5287790.1"
                     /translation="MYFVLLYDLVDDYLERRAPLREQHLKLAREAEARGELVLGGALA
                     DPADRSIIVFKGDDASVAERFAKADPYVHNGLVKKWTVRKWSLVVGSYLPK"
     gene            3294..3701
                     /locus_tag="VFV20_05260"
     CDS             3294..3701
                     /locus_tag="VFV20_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287791.1"
                     /translation="MSFWLLGSVALANPSADADCATTVTDPFLGTGTRADLYGCKSIG
                     NSGDEQGIYVINADAADLGYPADYGTCSVLVKWSGNYGLTPYLDYGTVYNQTKCSSGY
                     VEIWGDRFDATDGYGATPYVYSIGGQGTQLHPH"
     gene            3760..>4405
                     /locus_tag="VFV20_05265"
     CDS             3760..>4405
                     /locus_tag="VFV20_05265"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEU5287792.1"
                     /translation="MEFPQRLTPAPPRGFRDILPTEARELRAIEHALTTTFTAYGYMP
                     LEPPMVEHTAGESILAGDRLIQFLDRDGSLVALRPDITTAVARLVAQRYADTSGALRL
                     SYFAPVFREEPAMLGAEREFSQAGVELVGPSGPLADAEALALLAESLARAGLRDETIE
                     IGHVGVVRALFSELAEDARELVMRELRDGDHVGAFRRAREGGMNDAALMRARSAL"
BASE COUNT          708 a         1559 c         1555 g          583 t
ORIGIN      
        1 cgcgaccggt gcgaccggcg cgggatgccc gacgggcgcg acgggtgcga ccggtgcgac
       61 cggcgcgacg ggtgcgaccg gcgcgacggg tgccaccggc gcgacgggtg ccacgggcga
      121 ggcgacaggc aagcccgacc agcagggtcg cgtgacggtc gcccagcgca ccacgctcga
      181 ggcgaacatc cagtcgatcg tcgacctcgc catcaaggac atggacgacc tcgtgaagtc
      241 ggcgaccacc gaggcggccg ccgtcctccc agcgcagcac ggcaattcgg gcgataacgg
      301 caagtccggc gacaaccccg gcaacaagcc cgagaacgcg ggcaagccga ccggttcgcc
      361 aaagcgctaa agcaccttct cccatctcct gacgcgaaac gcccgccgct cggcgggcgt
      421 tttgcgtagc gcccggcgag gaaccgcggg gcgcgtcacg ttgtaaagac ctccagaaca
      481 ggcgggaggt ctcgcatgcg catcctcatc gcgacgctcg ctctcatgct cgccctcggg
      541 acgaccgccc tggccggcgg atgggcggtg acgacgctcg acacgctgcc accggacatc
      601 agcgcgggcc agacgtacac catcggctgg acgatccgtc agcacggcgt cacacccgtc
      661 gacgtcgaac gcctcggtgg gacgacggag ctcatcgcga catcgcccga cgggtcgcgc
      721 acggtgtcct tcgcgggacg acgcgacggc gacaccggtc actacgcggc gaaggtcacg
      781 ttcccgagcg agggcacgtg gacctggaag gtgactcagg ccccgttcca gccgcagccc
      841 ctcggcaccg tcgtcgtttc cccagcgctc gccggcagtg acgccgcgcg gggcgtgaac
      901 gccgcgaaca cggccgcatc gggcgcaccg gacccgatct cgaatccgtg gctcgtcctc
      961 gcgctgctcc tcgcctcggg cggcgcggca gttctcttcg gcacacgcgt ggccgcgctg
     1021 acggtgcgac cccggagcgc gtgacgagca tgcgccgcga ggttgcagac gtcgttggtt
     1081 ccgggctgct cctcgcggcg ctcgtcacgg cgacgatcgg cgtcatgacc gatcgcaccg
     1141 catcggcgag cggctcgtcg ccatccacca ccgtatcgct caccgacgcg agcctcgtcg
     1201 cgcgcggcag gtcgctcttc agcgcgaagg gctgcgtcgc gtgccacacc aaggagcagc
     1261 aggccatcgt ccaggtcgga cccgcgctca ccggcctgcg cgaccgcgcc gccgaacgga
     1321 ggcccggtct ggatgccgtc gcgtacgtgc gcgaatcgat ccgcacgccg gcggcgttca
     1381 tcgtgcccgg ctacggcaac gcggccatgc ccgacctcgc gctccgtgac gaagatatcg
     1441 acgcgatcgc ggcgtacctc ctcggcacgc cctgaggtca gcgtacggcg acgcggaccg
     1501 cgtggcgcag cagctcgagc gcccgctcca catcggcggc cctgcgcacg cgcaccagga
     1561 tccaatcgaa ccccgggccg cgcaggtcga tgcgcgcgtc gcttcgtagc tcgtcgcgca
     1621 gctcgcgcac gacctggcgg gtaagccgca cgtcgacctg gccatcgtgg caatgcgcca
     1681 cctcgcgcgt gccgtaccac agcgcgtcgg tgtgtcgcgg tcccttgaag cgcgacggcc
     1741 gcacggtcac gccgcgcatg cgcgcgccgc gcttcatgaa ctgggcgagc atcgcgcgat
     1801 ggtaggtgag cgactcagga gaagacgccg agcagaccga gcagaccggc gccgacgacg
     1861 atcacgaacg ggctgagacg cagtacggtc gacgcgagca tcgcgacgac gagtccgacg
     1921 acgtgcgtcg ggagcggcag cagcggtgtg ccgagcgagt acaggctcgc gccgatgagg
     1981 ccgacgctga gcgggacgag cgtccggtcg agcgcgcgga tccacggcga gcgcgagaga
     2041 cgttcccact gcgacgcgac gacgacctgg agcaggagcg gcggcacgag gaaggcgacg
     2101 agcgcgacga ccgccccggg tgcgccggcc gcgtagaagc cgagcgggac cagaaagagg
     2161 atgcccgggc cgggggtggc ctggccgatc gcgtaggcgt tgaggaactg ctgctgggtc
     2221 atcgccgcgc gctgatcgac cagctcgtgg tcgaggtccg cgaggaccga cgcgccgccg
     2281 ccgaagctca ccgcaccgac gcgaaggaag agcagcgcga cgtcgacgag cgtcacggca
     2341 gcgtcagccg ggaccagcgg tagcgcagga cgccgatccc gcccacgatg aggaccgcgg
     2401 ccaccatcgg cgtgcccgcg acgatgagga tcagcgtcgc ggcggtcagc agcgcgtcga
     2461 aggaccgctg gagccgtcgt gtcatgcgca cgaacgacgc ggcgaggacg ccgaccgcgc
     2521 cgacgaggat gccctggaag aggcgcgcca cccacggctc gctcatcgac aggaacgcga
     2581 ggacggcggt cacggcgacg aggccgggga gcaggacgag cacgatcgcg aggaacgcgg
     2641 tgcgcggccc gccgaggcgc atgccgcaga gcgcggtgag gttcacgaag cccggacccg
     2701 gaagcgtctg cgcgatcgcg aggtcgcgca tgaagtcctc ttcgcggatc gcacccgtcc
     2761 gcacgaaggc gttctcgagg ttcacccagc gcgccgcgcc gaaggcggtg aggccgaccc
     2821 agaggaactt gccgagcacg ccgccgaagc gcatgcgctc atactgtcgt acgccgatgt
     2881 acttcgtcct gctgtacgac ctcgtcgacg actacctcga gcgccgcgcg ccgctgcgcg
     2941 agcagcatct gaagctcgcg cgcgaggcgg aggcccgcgg tgagctcgtc ctcggcggcg
     3001 cgctcgccga tcccgccgac cgctcgatca tcgtgttcaa gggcgacgac gcgtccgtcg
     3061 ccgagcgctt cgccaaggcc gatccctacg tgcacaacgg gctggtgaag aagtggaccg
     3121 tgcggaagtg gtccctcgtc gtgggctcct acctgccgaa gtaggcagcg tgcgacgtcg
     3181 caccgacgtg ttgtgtccaa gtcatttcga tgtgcgcgcg tatgctgcgc gccaacacgc
     3241 actgtgggga ggtgggtatg cggcgcttcg ttctcatcgc gacttcgtgc ttggtgtcct
     3301 tctggcttct cggtagtgtt gctctcgcga atccgagcgc ggacgcggac tgtgcgacga
     3361 ccgtcaccga tccgttcctc ggaaccggaa ctcgtgcgga tctgtacggg tgcaagtcga
     3421 tcggcaactc cggcgacgaa cagggcatct acgtgatcaa cgccgacgcc gcggatctcg
     3481 gctacccggc cgactacggg acgtgcagcg tgctggtgaa gtggtcgggc aattacggcc
     3541 tcacgcccta cctggactac ggcacggtct acaaccagac caagtgctcg agcggatacg
     3601 tcgagatctg gggcgatcgt ttcgacgcga cagatggcta cggcgccacc ccctacgtgt
     3661 attcgatagg cggtcagggc acccagctcc atccgcacta gccggtaggc gcgacgggtg
     3721 cgccggtcgg cgtgcccgtc gcgcgctacg ctcccgtttg tggagttccc acagcgcctg
     3781 acgccggcgc cgccgcgcgg cttccgcgac atcctcccca ccgaggcgcg cgagctgcgt
     3841 gcgatcgagc acgcgctgac gacgacgttc acggcgtacg gctacatgcc gctcgagcca
     3901 cccatggtcg agcacaccgc gggcgagtcg atcctcgccg gcgaccgcct catccagttc
     3961 ctcgaccgcg acggcagcct ggtcgcgctg cgtcccgaca tcacgacggc cgtcgcgcgg
     4021 ctggtcgcgc agcgctatgc ggacacgagt ggtgccctgc gcctctccta cttcgcgccg
     4081 gtcttccgcg aggagcccgc gatgctcggc gccgagcgcg agttcagcca ggccggtgtg
     4141 gagctggtcg ggccgtcggg acccttggcc gatgccgagg cgctcgcgct cctcgcggag
     4201 tcgctcgcgc gcgcggggct gcgcgacgag accatcgaga tcggacacgt gggcgtcgtg
     4261 cgcgcgctct tcagcgagct cgccgaggac gcgcgagagc tcgtcatgcg cgagctgcgc
     4321 gacggcgatc acgtcggcgc gttccggcgg gcgcgcgagg gcggcatgaa cgacgcggca
     4381 ctgatgcgcg cgcgctccgc gctcg
//
LOCUS       DASTZR010000236         2832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_431111, whole genome shotgun sequence.
ACCESSION   DASTZR010000236 DASTZR010000000
VERSION     DASTZR010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431111"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1612
                     /gene="gltX"
                     /locus_tag="VFV20_05270"
     CDS             167..1612
                     /gene="gltX"
                     /locus_tag="VFV20_05270"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU5287793.1"
                     /translation="MTVRVRFAPSPTGSLHIGSVRTTLYNYLFARQNKGTLVLRIEDT
                     DQDRLVPGAIDSIYDGLEWMGITWDEGPREGGDDAPYVQSERLPMYQKAAQELVTKGA
                     AYFCFCSKERLAALRAQQAARKEITRYDGLCRTIAPAEAAERAKNEPHVVRLRVPDEG
                     VIALDDLVYGRIEWPIATIEDQVLLKSDGYPTYHLAVVVDDHVMGITHIFRGEDWLPS
                     TPKHLLIFRAFGWDVPPIAHFPNVLGADNKKLAKRHGATKVEQFRADGYLPEAMINYL
                     ALIGWAPGTEDEIFSLDDLVARWRIDQVQKAGGKWDRARLDWFNGVWIRKLTLEALME
                     RLAPYVPAEWDRGVLTRALPLIHERMATLVEARDQLAFLFTDDLAYDPALLVQENDRA
                     RSVRSLTRTASALRGLASFDAAAIKAAIEGVAAELGWKVKPVTLVIRAAVTGRRVGPP
                     LYESLELLGRDRALARIARAEELLGEGPRDA"
     gene            1677..>2832
                     /locus_tag="VFV20_05275"
     CDS             1677..>2832
                     /locus_tag="VFV20_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5287794.1"
                     /translation="MRRARAAGLESVWFHDSYFERDAVTYATAVASQVDGIKVGLGAL
                     NPFTRHPVLIAMTISALDEIAPERVLLGLGSALPLRLGQMGVPYAPDEAATRVATAID
                     TLHTLWSGERMPSGKPGLPPLVPQFPPVHRVPIYIAGYRSPMMVVAGQKADGYLARPA
                     ESIPGLRKLLRVMDRSAREAGRDPDDIDVAGYLLTLIADTRRDAINRAKREPFVIYMM
                     SILSDVTLKRAGFETALRDQIAAAWRKEDFTTAGSLIPDELLDAFILCGTRREVADRA
                     WEYHEAGMDLPLLQPVVQDEEQTLAVIDAAVAYGSAETGAVAARAALGAQRKSAFASA
                     RDRLAAYYEIIRPFSFTASSAPVAVAGALAAFEGIFSWPLFLTSLAASVLL"
BASE COUNT          416 a         1047 c          946 g          423 t
ORIGIN      
        1 agatgacgac gcccagcgcg atccagacct gaccgatgcc tcgcgagccg ccgccgccac
       61 caccggtggc gcgccgctgc tgctggcgtc gctgttgctg gcgccgttca cggcgcgtcg
      121 ttaccgtcat cccgtccgaa cagcataccc acctaaactg agattcgtga ccgtccgcgt
      181 gcgcttcgcc ccatcaccca ccggctccct gcacatcggc agcgtgcgca cgaccttgta
      241 caactacctc ttcgcccgcc agaacaaggg gacgctcgtc ctccggatcg aggacaccga
      301 tcaggatcgc ctcgtcccgg gcgccatcga ctccatctat gacggcctcg agtggatggg
      361 catcacctgg gacgagggac cgcgcgaggg cggcgacgat gccccgtacg tccagtcgga
      421 gcgcctgccc atgtaccaaa aggcggcgca ggagctcgtg accaagggcg ccgcgtactt
      481 ctgcttctgc tcgaaggagc ggctggcggc gcttcgcgcg caacaggcgg cccgcaagga
      541 gatcacgcgc tacgacggcc tctgccgcac catcgcgccg gccgaggcgg ccgagcgcgc
      601 gaagaacgag ccgcacgtcg tgcgcctccg ggtgccggat gagggcgtga tcgcgctgga
      661 cgacctcgtc tacggacgca tcgagtggcc gatcgccacg atcgaggacc aggtgctgct
      721 caagtccgac ggctacccga cgtatcacct cgccgtcgtc gtcgacgacc acgtcatggg
      781 gatcacgcac atcttccgcg gcgaggactg gctcccgtcc acgcccaagc atctcctgat
      841 cttccgcgcg ttcggctggg acgtcccgcc catcgcgcac ttccccaacg tgctcggcgc
      901 cgacaacaag aagctcgcga agcggcacgg cgcgaccaag gtcgagcagt tccgcgccga
      961 cggctacctg cccgaggcga tgatcaacta cctcgcgctg atcgggtggg cgccgggcac
     1021 cgaggacgag atcttctcgc tggacgatct cgtcgcgcgc tggcgcatcg atcaggtcca
     1081 gaaggccggc ggcaagtggg accgcgcgcg cctcgattgg ttcaacggcg tgtggatccg
     1141 gaagctgacg ctcgaggcgc tgatggagcg gctcgcgccg tacgtcccgg ccgagtggga
     1201 ccgcggcgtg ctcaccaggg cgctgccgct gatccacgag cgcatggcca cgctggtgga
     1261 ggcgcgcgac cagctcgcgt tcctcttcac cgacgacctc gcctacgatc ccgcgctgct
     1321 cgtccaggag aacgatcgcg cgcggtccgt ccggtcgctc acgcgcacag cgagcgcgct
     1381 gcggggtctt gcgtcattcg acgccgcggc gatcaaggcg gcgatcgaag gggtcgcggc
     1441 ggagctcggc tggaaggtga agcccgtcac gctggtcatc cgcgccgcgg tcaccggccg
     1501 cagggtcggt ccgccgctgt acgaatcgct ggagctgctc ggtagggacc gcgccctcgc
     1561 gcgcatcgct cgcgcggagg agctgctcgg ggagggacca cgagatgcct aacctcgctc
     1621 gtcgcgtcgg actcggcctc gcagcgcgcg gatccgtctc ggacacagtg ggctgggtgc
     1681 gccgcgcccg cgcagccggc ctcgagtcgg tctggttcca cgattcgtac ttcgagcgtg
     1741 acgccgtgac ctacgcgacg gccgtcgcgt cgcaggtgga cggcatcaag gtgggactcg
     1801 gtgcgctcaa ccccttcacc cgccatcccg tcctcatcgc gatgaccatc agcgcgctcg
     1861 acgagatcgc gcccgagcgc gtcctcctcg gcctcgggtc ggcgctgccg ctgcggctcg
     1921 gccagatggg cgtgccctac gcgccggacg aggccgcgac gcgcgtcgcg acggcgatcg
     1981 acacgctgca cacgctctgg tccggagagc gcatgccctc ggggaagccc ggcctgccgc
     2041 cgctcgtgcc gcagttcccg ccggtgcacc gcgtccccat ctacatcgcc ggctatcgct
     2101 cgccgatgat ggtcgtcgcg ggccagaagg cggacggcta cctcgcgcgg cccgccgagt
     2161 ccattcccgg cctccgcaag ctgctgcgcg tcatggaccg ctccgcgcgc gaggccggcc
     2221 gcgatcccga cgacatcgac gtcgcgggct acctcttgac gctgatcgcc gacacgcgcc
     2281 gcgacgcgat caaccgcgcg aagcgcgagc cgttcgtcat ctacatgatg tcgatcctct
     2341 ccgacgtcac gctgaagcgc gccggattcg agaccgcgct gcgtgaccag atcgcggcgg
     2401 catggcgcaa ggaggacttc accaccgccg gttcgctcat ccccgacgag ctgctcgacg
     2461 cgttcatcct ctgcggcacg cgccgtgaag ttgccgaccg cgcgtgggag taccacgagg
     2521 ccgggatgga cctgccgctg ctgcagcccg tggtgcagga cgaggagcag acgctcgccg
     2581 tgatcgacgc cgccgtcgcg tacggcagcg cggagaccgg cgccgtcgcg gcgcgcgcgg
     2641 cgctcggcgc acagcgcaag tccgcgttcg cgagcgcgcg cgatcgcctc gcggcctact
     2701 acgagatcat ccggccgttc tcattcaccg cgtcgtccgc gccggtcgcc gtcgcgggcg
     2761 cgctcgccgc gttcgagggg atcttcagct ggccgctctt cctcacctca ctcgccgcga
     2821 gcgtgctgct gc
//
LOCUS       DASTZR010000237         3679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_431119, whole genome shotgun sequence.
ACCESSION   DASTZR010000237 DASTZR010000000
VERSION     DASTZR010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431119"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VFV20_05280"
     CDS             <1..802
                     /locus_tag="VFV20_05280"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU5287795.1"
                     /translation="MKYGAKGPNMAHVSACASSAHAIGEAAEMIRRGQADVMLAGGAE
                     AGLIKIAIAAFNQARALSTRNDAPDKASRPFDRDRDGFVFSEAAGCLVLEDLEHARAR
                     GARILGEVIGYGATADAYHVTAPAEGGEGAVRAMRMAVADAGIAPADVGYINAHGTST
                     QANDGSESTAIKTVFGKHAYELAVSSTKSMIGHTLGAAGAIEAIFCLLAMRDGVLPPT
                     INLENPDPVCDLDYVPNAARTQQVEISMSNSMGFGGHNASLLFRRAAV"
     gene            802..1278
                     /locus_tag="VFV20_05285"
     CDS             802..1278
                     /locus_tag="VFV20_05285"
                     /inference="COORDINATES: protein motif:HMM:NF005457.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HEU5287796.1"
                     /translation="MADPKKLRADANALAVELLHRLSSDEVQELAVRRGAVRVRVSKA
                     AVAAVADPRAGAPAADVRAGAGAAPIVATPDAPAQPDRPTVNAPLTGIFYRSPSPQAP
                     PFIQVGSQVTQGEVIGLIEAMKLFNEIRATTGGRVRRILIENGQLVRAKQPILELE"
     gene            1314..1469
                     /locus_tag="VFV20_05290"
     CDS             1314..1469
                     /locus_tag="VFV20_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287797.1"
                     /translation="MVCKQPPGEGALRLRYEGRYYDFDRERCKRIFQENPDRWLDASG
                     DVLEQPR"
     gene            complement(1475..2698)
                     /locus_tag="VFV20_05295"
     CDS             complement(1475..2698)
                     /locus_tag="VFV20_05295"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEU5287798.1"
                     /translation="MRRLRFYLLALGPGLVTAFADNDAGGITTYSIAGAQFGFGLMWV
                     LLVTAVALTVTQEMGARLALATGQGLAGLIRERFGVRWTAFAVLTMLIGNLGTTIAEF
                     AGMAAALGLFGVPPQLSAVLSAALVVLILARGNFTRIQYAFVAIGAGVSIAYAASAIL
                     SNPDWAHATGALVRPEIAWSNAYFLMLVGVVGTTITPWGQALIQSYIADKGLGPRDLG
                     PVRLDVTLGSGILTNLIAAFIVVACAATLFPRGETINDAADAARALGPLVGPSATTVF
                     AVGLFAAGLLGLGTVPLSSAYFACEAFGFERGLSFKWREAGVFYGLLTIFIAGAALFV
                     VIPGLPLIQVMLLAQVVNGLLLPIILIFVMRLSREPNLGALRSGPILYPLGWLITALA
                     SLMSLAFVASLFLGR"
     gene            complement(2695..>3679)
                     /locus_tag="VFV20_05300"
     CDS             complement(2695..>3679)
                     /locus_tag="VFV20_05300"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5287799.1"
                     /translation="KQIVDTHGAKVVRVNDLQLSEVDGQLRLVGADVGLRGLLRRVGA
                     EAFAERFAGMAGRKLPRGIIPWHLVEPLEAPDAAVRVTVPRTKLALLHPADIADIVEE
                     MAADERRQVFEQLDVETAAEALSEVEPEMQASIVSDLDEERAADILEEMAPDEAADLL
                     QDLPEERREELVDLMQKEEAADVEELLTYDEESAGGIMTTDFVALAADLTAAQAIDKL
                     RELQPDPELTYYLYVVDAQGRLDGVISLRDLVVAKPDQKLAAIMDPHVLKVEATTTKE
                     DVASLIAKYDLLALPVVDARQKLLGAVTVDDVVEIMLPRGWKKRSSRTLAR"
BASE COUNT          594 a         1253 c         1342 g          490 t
ORIGIN      
        1 gatgaagtac ggcgcgaagg gtccgaacat ggcgcacgtg tccgcgtgcg cctcgagcgc
       61 ccacgcgatc ggcgaggcgg ccgagatgat ccgccgcgga caggcagacg tcatgctcgc
      121 cggcggcgca gaggccggcc tcatcaagat cgcgatcgcg gcgttcaacc aggcgcgcgc
      181 gctctcgacg cgcaacgacg cgccggacaa ggcctcgcgg ccgttcgacc gtgaccgcga
      241 cggtttcgtg ttcagcgagg cggcgggctg cctcgtgctg gaggacctcg agcatgcccg
      301 cgcgcgcggc gcgcggatcc tcggggaggt gatcggctac ggtgcgaccg ccgacgccta
      361 ccacgtcacc gcgccggccg agggcgggga gggtgccgtg cgcgcgatgc gcatggccgt
      421 cgccgacgcg gggatcgcgc cggcggacgt cggctacatc aacgcgcacg ggacgtcgac
      481 gcaggcgaac gacggatcgg agtcgacggc gatcaagacg gtgttcggga agcacgccta
      541 cgagctcgcc gtgtcgtcga caaagtccat gatcgggcac acgctcggcg ccgcgggtgc
      601 gatcgaggcg atcttctgtc tgctcgcgat gcgggatggc gtgctcccgc cgacgatcaa
      661 cctggagaac cccgaccccg tgtgcgacct cgactacgtg ccgaacgcgg cacgcacgca
      721 gcaggtcgag atctcgatgt ccaactcgat gggcttcggc gggcacaacg catcgctgct
      781 cttccgccgg gccgcggtct gatggccgac ccgaagaagc tgcgcgccga cgcgaacgcg
      841 ctcgcggtcg agctgctcca tcgcctgtcg tccgacgagg tccaggagct cgcggtgagg
      901 cgcggcgcgg tgcgcgtccg tgtgagcaag gccgccgtcg cagcggtcgc ggacccgcga
      961 gccggtgcgc cggccgcgga tgtgcgtgcc ggggcggggg ccgcgccgat cgtcgccaca
     1021 ccggatgcgc cggcgcagcc ggatcgtccc accgtgaacg cgccgctcac cggcatcttc
     1081 taccgctcgc cctcgccgca ggcgccgccg ttcatccagg tgggcagcca ggtcacgcag
     1141 ggcgaggtga tcggcctcat cgaggcgatg aagctgttca acgagatccg cgcgacgacg
     1201 ggcggacgcg tgcgccggat cctcatcgag aacggtcagc tcgtccgcgc gaagcagccc
     1261 atcctcgagc tcgagtgatg gacgacgccg ggcgaggagc gggtcggcgc tgcgtggtct
     1321 gcaagcagcc acccggagag ggcgcgctgc gcctccgcta cgagggccgg tactacgact
     1381 tcgatcgcga gcgctgcaag cggatctttc aggagaaccc cgaccgctgg ctcgacgcga
     1441 gcggggacgt gctcgaacag ccgcgctagc gaacctagcg accgaggaac agtgacgcca
     1501 cgaacgcgag cgacatgagg ctcgcgaggg ccgtgatgag ccagcccagc ggatagagga
     1561 tcgggccgct ccgcagcgcc ccgaggttgg gctcgcggga cagccgcatg acgaagatga
     1621 ggatgatcgg cagcagcagc ccgttcacga cctgcgcgag gagcatgacc tggatgagcg
     1681 gcaggccggg gatgacgacg aagagcgcgg cgcccgcgat gaagatggtg agcaggccgt
     1741 agaagacgcc ggcctcgcgc catttgaacg agagtccgcg ctcgaacccg aatgcctcgc
     1801 acgcgaagta cgcgctcgag agcggcaccg tcccgaggcc gagcaggccc gcggcgaaga
     1861 ggccgaccgc gaacacggtg gtcgccgatg ggccgaccag cggtccgagc gcgcgcgccg
     1921 cgtcggccgc gtcgttgatc gtctccccgc gcgggaacag cgtcgcggcg cacgccacga
     1981 cgatgaacgc ggcgatcagg ttcgtgagga tgccggaacc gagcgtgacg tcgagacgta
     2041 ccggcccgag gtcgcgcggc cccagcccct tgtccgcgat gtacgactgg atgagcgcct
     2101 gaccccacgg agtgatcgtc gtgcccacga caccgacgag catgaggaag tacgcgttgc
     2161 tccacgcgat ctcggggcgc acgagggcgc cggtcgcgtg cgcccagtcg gggttcgaga
     2221 ggatcgcgga cgccgcgtat gcgatggaga cgcccgcacc gatggcgacg aacgcgtact
     2281 ggatcctcgt gaagttgccg cgggcgagga tgagcaccac gagggcggcc gagaggaccg
     2341 ccgagagctg tggcggcacg ccgaagaggc cgagcgccgc ggccatgccg gcgaattcgg
     2401 cgatggtcgt gccgagattc ccgatgagca tcgtgaggac cgcgaaggcg gtccagcgca
     2461 cgccgaagcg ttcgcgaatg agtcccgcga gcccctggcc cgtcgcgagc gcgagacgcg
     2521 cacccatctc ctgcgtcacg gtgagcgcga ccgccgtcac gagcagcacc cacatgaggc
     2581 cgaagccgaa ctgcgccccc gcgatcgagt aggtggtgat gccgccggcg tcgttgtccg
     2641 cgaacgccgt gacgagaccg ggaccgagcg cgaggagata gaagcggagc cgcctcaacg
     2701 cgcgagagtc cggctcgacc tcttcttcca gccgcgcggg agcatgatct ccacgacgtc
     2761 gtccacggtg accgcaccga gcagcttctg acgcgcgtcg acgacgggca gcgcgagcag
     2821 gtcgtacttc gcgatgagcg acgcgacgtc ctccttggtg gtcgtggcct cgaccttgag
     2881 gacgtgcgga tccatgatcg cggcgagctt ctgatcgggc ttcgcgacga cgaggtcgcg
     2941 caggctgatc acgccgtcga ggcggccctg cgcatccacc acgtagaggt agtacgtgag
     3001 ctccggatcg ggctgcagct cgcggagctt gtcgatggcc tgcgccgcgg tgaggtcggc
     3061 ggcgagcgcg acgaaatcgg tcgtcatgat cccgcccgcc gactcctcgt catacgtgag
     3121 gagctcctcg acgtccgcgg cctcttcctt ctgcatgagg tcgacgagct cttcgcggcg
     3181 ctcctcggga aggtcctgga gcaggtcggc ggcctcgtcc ggcgccatct cctcgaggat
     3241 gtcggccgcg cgctcctcgt cgaggtcgct gacgatggac gcctgcatct cgggctcgac
     3301 ctccgacagc gcctcggccg cggtctcgac gtcgagctgc tcgaacacct ggcgccgctc
     3361 gtccgcggcc atctcctcga cgatgtcggc gatgtccgcg gggtggagga gggccagctt
     3421 cgtgcgcggg accgtgacgc gcaccgccgc gtcgggtgcc tcgagcggct ccacgaggtg
     3481 ccacgggatg atgccccgcg ggagcttgcg gccggccatc ccggcgaagc gctcggcgaa
     3541 agcctcggcg ccgacccggc gcagcagccc gcgcaggccc acgtcggcgc ccaccagacg
     3601 gagttggccg tccacctccg agagctggag gtcgttgacc cggacgacct tggcgccgtg
     3661 ggtgtcgacg atctgcttg
//
LOCUS       DASTZR010000238         9092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_639839, whole genome shotgun sequence.
ACCESSION   DASTZR010000238 DASTZR010000000
VERSION     DASTZR010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9092
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639839"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..1637
                     /locus_tag="VFV20_05305"
     CDS             138..1637
                     /locus_tag="VFV20_05305"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU5287800.1"
                     /translation="MKLAALFGASGIAVPEGAGDIEVRTLAYDSRKAAPGAVFVAIRG
                     FHRDGHAFVADALARGASAVVAEEPVPSTAPLAVVADTRAALADLAAELFDHPTRKLK
                     IVGVTGTDGKTTTVHLVSDVLEARGERTGFATTVDFKLAGSAWANETRQSTQEAVEIQ
                     EFFAELLVSGGTWGVLEATSHALALRKVRGCEIDIAVFTNLTPEHLDFHGTLQSYLDA
                     KALLFEMLTTSVDKGVPKAAVLNADDPHWRYLADRAAPSRVLTYGIDAHADLQGTVLS
                     ADANGSLVRVAYHDDTIELALPLVGRFNVHNALAAMAAGIAAGVPLATAKTALEQAQP
                     VRGRMERVVCGQPFTVIVDYAHTPESLDKVLALLRPLTAGRLIAVFGSAGERDRSKRP
                     RLAEVASRYADLFVITQEDPRLEDPGAILSEIEAGAKSAGKRPGSDYLVIDDRAAAVR
                     EAIGRAGPGDTVLLAGKGHEGSIIVGEEKRPWDEAGVARDALRDLGYAA"
     gene            1637..2302
                     /locus_tag="VFV20_05310"
     CDS             1637..2302
                     /locus_tag="VFV20_05310"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5287801.1"
                     /translation="MARSLKRSHTVRLSGAATRAQTRTLSAHRRSLTQQLREEIGVPA
                     EPAGTAALVMLMGFPGVGKSHCARLLAERLRAAHVATDHLRSRLFIAASYADAENDAV
                     FGIAEALVDELLVERHVVVLDATHLVARNRAPAERVARDRGARLFHVLITADDAATRE
                     RLAARSRERASGDHSDADERVYERMRARAVELPAGGYLEIHNGPNVADEVARVAAMIE
                     APR"
     gene            2299..3354
                     /locus_tag="VFV20_05315"
     CDS             2299..3354
                     /locus_tag="VFV20_05315"
                     /inference="COORDINATES: protein motif:HMM:NF022113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HEU5287802.1"
                     /translation="MSARAALFGAIAVVLLVFYGIVGSRLSPVLVPGSTITPPPPKPS
                     SAVPAPRVPGTIAFTLRGDIYVLRDGKYVNVTSEGRNLTPALSVDGRTLLFSRTETID
                     GRRSVDGQVVPALLHYTDIIRKDSTGGTETILVTGLKTKSAAGFHVVAYHDEPALAPD
                     GSKFAIVTDFGDGNGSNLEVYDAKSTGSTPRRLALLSQGSELADPSWSPDGKTIVVTS
                     YTLGAPRLLVVPVDGRAAVPQKVGAEGEAYRASYSSDGRWLIYTLRRPTGGNDVHAVE
                     ISSGRDIALTSDGKSWNGVFSPDGSQVAFLRETGGVIDVYAMELGGALTGGTPKAPVK
                     LTNGEGIDGGSRPSWGG"
     gene            complement(3356..4207)
                     /gene="phnE"
                     /locus_tag="VFV20_05320"
     CDS             complement(3356..4207)
                     /gene="phnE"
                     /locus_tag="VFV20_05320"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEU5287803.1"
                     /translation="MAEYFRARNMPLPPELERTILTDWRRYLVLLVTAAALWVGLQIT
                     AIDTSRANPGYVAQIIGQLLAPDWNVFFPQNEFETFWDAGIGLMVITIFMAIIATAFA
                     VALAVPLSFLGARNVMTGNPITSAVYAIVRVFFTVVRSIDILILAVVAVVLFSTGNAA
                     GVFAIAFHNVGVMGKLYSEAVEGIDPGPIEAITATGANRFQVIWTAVLPQIVNPYISL
                     TIYRLDTNVRLAPVLGLMGAGGIGTLLFQNMNLLRYHASGALIFLIVITVVVMDFFSA
                     QVRKRLA"
     gene            complement(4230..5072)
                     /gene="phnC"
                     /locus_tag="VFV20_05325"
     CDS             complement(4230..5072)
                     /gene="phnC"
                     /locus_tag="VFV20_05325"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011246101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HEU5287804.1"
                     /translation="MPSVLTITDLTKIYPGGTRALDGISLDIERGEFVVLIGLSGSGK
                     STLLRCLNRLVEPTSGRVVFDGIDVTAARGAQMRHVRRRMGMIFQQFNLVKRASVFHN
                     VLAGRLGYRSTWRTIASRPGHDDIDLAFTNLARVGILEKAYARADALSGGQQQRVGIA
                     RALMQRPDLMLADEPVASLDPATSHSVMKYLEEINRKDGITVICALHFLSLARRYGTR
                     VIALKGGKVAFDGKPGDIDERRFKEIYGEDAVEVEIGPGKTRDEILSEAEALVKAPDL
                     TPDR"
     gene            complement(5155..6180)
                     /locus_tag="VFV20_05330"
     CDS             complement(5155..6180)
                     /locus_tag="VFV20_05330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU5287805.1"
                     /translation="MRLSLRGPLGWALAAGLVIAACGGTPTSTTPTASAPAASAAPSG
                     PRLGSFDRPIVLSFTPSQDTQRVTASGNAITASLERATSLKWKVSVPTSYAATIEGMC
                     AGSVDVAFLAPLQMALALDRGCATPFLGALRNDDTGKPSTTYKSQILVRSDSGINDIN
                     GLKGKKFAFVDTLSASGYLFPSLKIKEKTSQDPKTFFAANGIIFAGGHDKAALQVYQG
                     AVDGAATFIDVRTSAGMPADIMQKTKVIDTAGPIPNDGAAVRKGFPADLMTQIQKAMA
                     DYSQTDAGKKAFKDLYSWDGMQVVDSKFYDPVREAAKLAGIDLAGEAAKTARPVATPS
                     PSPTKAP"
     gene            6228..7052
                     /gene="pyrF"
                     /locus_tag="VFV20_05335"
     CDS             6228..7052
                     /gene="pyrF"
                     /locus_tag="VFV20_05335"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEU5287806.1"
                     /translation="MSFLDKLATATRARDSIVCVGLDPEPALIPEALGRGPQAALRFL
                     RRIVRATAEYVCCYKPNLAFYERYGGAAYDILGLTLQAIPDDIPVILDAKRGDVPNTS
                     EAYADALFDAFHADAATVAPYLGMDSIAPFARDDRFALVLARTSNPGARDLQDLRIDD
                     RPLYEHVVDACVRAFPRDRAGFVVGATYPEEARRLRELAPDRLFLMPGVGAQGGDVAT
                     ALRAALDASGGGVLPAASRSVIFASRGADYERAAADAARALRDAANAGRALAMERR"
     gene            7052..7357
                     /locus_tag="VFV20_05340"
     CDS             7052..7357
                     /locus_tag="VFV20_05340"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEU5287807.1"
                     /translation="MFGIGPEELMIVALIALLVLGPERMPKLMRDMGKVLGDLRKTSD
                     ELRDDFMNADKFLEAKAAAPAKPEPAPAESVPSGPPGESAFDREMRQAKERLAKPEA"
     gene            7427..8779
                     /gene="secD"
                     /locus_tag="VFV20_05345"
     CDS             7427..8779
                     /gene="secD"
                     /locus_tag="VFV20_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009634444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEU5287808.1"
                     /translation="MGRLGPWLIAIIAVLAIFIDIPKHQFSFPFNCPGICLKIGGFQV
                     NQEIKTHLGLDLQGGTQLILRMQLEKLPPGVTTPQSELQTQAIRVIDRRINSLGVSEP
                     VIQGLGDDKVLVQLPGVDDLQQANDIVTRQAFLEIKVPDKDKPGSFKSLEPPFTGANL
                     KPTFVTFQNGTKPVVNFEFGSPDDQRWVQLSKDYFDQPVQITLDTKEISAPTIRAIFT
                     DGRGIIEGNFTTQSAKELSLLLNSGALPVPLEVIQSSRVEPTLGQESVQRSLVAGAIG
                     LLLVAFFMIAYYRLPGAIAVIALLYYTALTYALFRLVPVTLTLAGVAGFILSIGMAVD
                     ANVLTFERLKEELRSGKSLRAAVEAGRVRAFPSILYSNLATILTAGILFWFGTGTVKG
                     FALTLAIGVAVSFFTAVIVTQMLLHAAIEIPSLRSRRLYGVEERQSEGREGRVAPVVS
                     "
     gene            8779..>9092
                     /locus_tag="VFV20_05350"
     CDS             8779..>9092
                     /locus_tag="VFV20_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287809.1"
                     /translation="MLHFVERKWMYFLLSGLLIVPGVVFLLLGGLRPGIEFKGGTLLD
                     ATFPGATPAVSDVREVMSTLGRGEAVVQAAEGGRIEIRTFEMSPEDVTKATKALQDKF
                     GAG"
BASE COUNT         1356 a         3120 c         3207 g         1409 t
ORIGIN      
        1 aagcgtggct tcggcggcgt cgtccggcac tgggtcggcg cgcacgacac cgtaccctcg
       61 ccgctgctct atcgcgcgta tctgggtgcc gagcccgcgt acacggcggt gctgcgcctc
      121 gctgggcgcg tgcgccggtg aagctcgccg ccctgttcgg cgcctccggt atcgccgtgc
      181 ccgagggcgc gggcgacatc gaggtgcgca cgctcgccta cgactcgcgg aaggccgcgc
      241 cgggagccgt cttcgtcgcg atccgcgggt tccatcgcga cggccacgcg ttcgtggccg
      301 acgccctcgc acggggcgcg tccgcggtgg tcgccgagga gccggtcccg tcgacggcgc
      361 cgctcgcggt ggtcgcggac acgcgcgccg cgctggcgga cctcgcggcg gagctgttcg
      421 atcatccgac gcgcaagctg aagatcgtcg gcgtgaccgg gacggacggg aagaccacga
      481 ccgtgcatct ggtgtccgac gtgctcgagg cgcgcggcga gcggaccgga ttcgcgacga
      541 ccgtcgactt caagctcgcg ggttcggcgt gggcgaacga gacccggcag agcacgcagg
      601 aggcggtgga gatccaggag ttcttcgcgg agctgctcgt gtcgggcggg acgtggggcg
      661 tgctcgaggc gacctcgcac gcgctcgcgc tgcgcaaggt gcgcggctgt gagatcgaca
      721 tcgccgtgtt cacgaacctc acaccggagc acctcgattt ccacggcacg ctgcagtcgt
      781 acctcgacgc gaaggcgctg ctcttcgaga tgctcacgac ctccgtggac aagggcgtgc
      841 cgaaggccgc ggtcctcaat gccgacgatc cgcactggcg gtacctcgcg gatcgggccg
      901 cgccgtcgcg cgtgctgacg tacgggatcg atgcccatgc cgacctgcag gggaccgtgc
      961 tgtcggccga cgccaacggc tcgctcgtgc gcgtcgcgta ccacgacgac acgatcgagc
     1021 tcgcgctgcc gctcgtgggg cgcttcaacg tccacaacgc gctcgcggcc atggccgccg
     1081 gcatcgccgc gggcgtaccg ctcgcgaccg cgaagacggc gctcgagcag gctcagccgg
     1141 tgcgcgggcg gatggagcgc gtcgtctgcg gccagccgtt cacggtcatc gtcgattacg
     1201 cgcacacgcc tgagtcgctc gacaaggtgc tcgcgctgct gcggccgctc accgcgggcc
     1261 ggctcatcgc ggtcttcggg agcgcgggcg agcgggaccg cagcaagcgg ccgcggctcg
     1321 ccgaggtggc gtcgcggtac gccgacctct tcgtgatcac gcaggaggat ccacgcctcg
     1381 aggatccggg ggcgatcctg tcggagatcg aggccggcgc gaagagcgcg gggaagcgcc
     1441 cgggcagcga ctatctggtc atcgacgacc gcgccgccgc ggtgcgcgag gcgatcgggc
     1501 gcgcggggcc cggcgacacc gtgctgctcg cgggcaaggg ccacgagggc tcgatcatcg
     1561 tcggcgagga gaagcgtccc tgggacgagg cgggcgtcgc gcgggacgcg ctgcgcgacc
     1621 tcggctacgc cgcgtagtgg cgagatcgct gaagcgctcg cacaccgtgc gtctctccgg
     1681 tgcggcgacg cgcgcgcaga cgcggacgct gtccgcgcat cgccgctcgc tgacccagca
     1741 gctgcgtgag gagatcggtg tgccggccga acccgcgggc accgccgcgc tcgtcatgct
     1801 catgggcttt cccggagtgg ggaagtcgca ctgcgcgcgg ctcctcgccg aacgcctccg
     1861 cgccgctcac gtcgccacgg accacctgcg cagccggctc ttcatcgccg cgtcgtacgc
     1921 cgatgcggag aacgacgccg tgttcggcat cgccgaggcg ctcgtcgacg agctgctggt
     1981 cgagcgtcac gtcgtcgtgc tcgacgcgac gcatctggtg gcacggaacc gcgcgcccgc
     2041 cgagcgcgtc gcgcgagatc gcggcgcccg gctgttccac gtcctgatca ccgcggatga
     2101 cgcggccacg cgtgagcgcc tggccgcgcg gtcgcgggag cgtgccagcg gtgaccactc
     2161 cgacgcggac gagcgcgtct acgagcggat gcgcgctcgg gcggtcgagc tccctgcggg
     2221 cggctacctc gagatccaca acgggccgaa cgtcgcggac gaggtcgcgc gcgtcgccgc
     2281 gatgatcgag gctccgaggt gagcgcgcgc gcggcgctgt tcggcgcgat cgccgtcgtg
     2341 cttctcgtct tctacggcat cgtgggcagc cggctctcgc ctgtgctggt gccgggcagc
     2401 acgatcacgc caccgccgcc gaagccgagc tccgccgtcc cggcgcctcg ggtgccgggc
     2461 acgatcgcct ttacgctgcg cggtgacatc tacgtgctcc gcgatgggaa gtacgtgaac
     2521 gtcacgtcgg aggggcgcaa tctcacgccg gcgctctcgg tcgacggccg aacgctcctc
     2581 ttctcgcgca ccgagacgat cgacggcagg cggagcgtgg acgggcaggt cgtgcccgcg
     2641 ctgctgcact acaccgacat catcaggaag gactccaccg gcgggacgga gacgatcctc
     2701 gtcaccggcc tgaagacgaa gtccgccgcc ggcttccacg tcgtcgccta tcacgatgag
     2761 ccggccctcg ccccggacgg gtcgaaattc gcgatcgtca cggacttcgg cgacggcaac
     2821 gggtcgaacc tcgaggtcta cgacgcgaag tcgaccggca gcacgccgcg gcgcctcgcg
     2881 ctgctgtcac agggatccga gctggcggat ccgtcgtggt cgccggacgg caagaccatc
     2941 gtggtgacct cgtacaccct gggcgcgccg cggctcctcg tcgttcccgt cgacgggcgc
     3001 gccgcggtcc cgcagaaggt cggcgcggag ggcgaggcgt accgcgcgtc ctactcgtcc
     3061 gacggcaggt ggctcatcta tacgctccgc cgcccgacgg gtgggaacga cgtccacgcc
     3121 gtcgagatct ccagcgggcg cgacatcgcg ctcacgagcg acggcaagag ctggaacggc
     3181 gtcttctcgc cggacgggtc gcaggtcgcg ttcctgcgcg agaccggcgg cgtcatcgac
     3241 gtctacgcga tggagctggg cggtgcgctc accggcggga cgccgaaggc accggtgaag
     3301 ctgacgaacg gcgaggggat cgacggcggg tcgcgcccgt cgtggggcgg gtgagctaag
     3361 ccaggcgctt gcggacctgc gccgagaaga agtccatcac cacgacggtg atcacgatca
     3421 ggaagatgag cgcgcccgac gcgtggtagc gcagcaggtt catgttctgg aagagcagcg
     3481 tgccgatccc gcccgcgccc atcagaccga ggaccggcgc gagacgcacg ttcgtgtcga
     3541 ggcggtagat cgtgagcgag atgtatgggt tcacgatctg tgggagcacg gccgtccaga
     3601 tcacctggaa gcggttggcg ccggtcgcgg tgatggcctc gatcggaccg ggatcgatgc
     3661 cctccacggc ctccgagtac agcttgccca tgacaccgac gttatggaac gcgatcgcga
     3721 agaccccggc ggcgttcccg gtgctgaaca gcaccaccgc gacgacggcc aggatgagga
     3781 tgtcgatcga gcgcacgacc gtgaagaaga cgcggacgat cgcgtatacc gccgacgtga
     3841 tcgggttgcc ggtcatgacg ttgcgggcgc cgaggaacga cagcggcacc gcgagcgcga
     3901 cggcgaacgc cgtcgcgatg atcgccatga agatcgtgat gaccatgagc ccgatgccgg
     3961 cgtcccagaa cgtctcgaac tcgttctgcg ggaagaagac gttccagtcc ggcgcgagca
     4021 gctgtccgat gatctgcgcc acgtagccgg gattcgcgcg gctggtgtcg atggccgtga
     4081 tctgcaggcc gacccagagc gccgctgccg tgaccaggag cacgaggtag cggcgccagt
     4141 ccgtcaggat cgtccgctcg agctccggcg gcagcggcat gttgcgtgcg cggaagtact
     4201 cggccactag cggccccgtc cgcgatcgat catcgatcgg gtgtgaggtc gggcgccttc
     4261 accagcgcct cggcctccga gaggatctcg tcgcgcgtct tgccgggtcc gatctcgacc
     4321 tccaccgcgt cctcgccgta gatctccttg aagcggcgct cgtcgatgtc gcccggcttc
     4381 ccgtcgaacg cgaccttgcc gcccttcagc gcgatgacgc gggtgccgta gcggcgcgcg
     4441 agcgacagga agtggagcgc gcagatcacg gtgatgccgt ccttgcggtt gatctcctcg
     4501 aggtacttca tcaccgagtg gctcgtcgcg ggatcgaggc tcgcgaccgg ctcgtcggcg
     4561 agcatgaggt cgggccgctg catgagcgcg cgcgcgatcc cgacgcgctg ctgctgtccg
     4621 cccgacagcg cgtccgcgcg cgcgtacgcc ttctccagga tcccgacgcg cgcgaggttg
     4681 gtgaacgcga ggtcgatgtc gtcgtggccg gggcggctcg cgatggtgcg ccacgtcgag
     4741 cggtagccga gccgacccgc gagcacgttg tggaacacgg aggcgcgctt cacgagattg
     4801 aactgctgga agatcatccc catgcggcga cggacgtggc gcatctgcgc cccgcgcgcg
     4861 gccgttacgt cgatgccgtc gaagaccacg cggccgctcg tcggctcgac gagccgattc
     4921 aggcaccgca gcagcgtgga cttgccggac ccggacaggc cgatgagcac gacgaactcg
     4981 ccgcgctcga tgtcgagcga gatcccgtcg agcgcacggg tgccgcccgg atagatcttc
     5041 gtgaggtcgg tgatggtgag gacggaaggc atctcgccgc aggatatcca tgaatgacga
     5101 aggccgccgg gatcaccggc ggccttcatc aggatcgcgt gtgtatgacg gccgctacgg
     5161 agccttcgtc ggcgacggcg acggggtcgc gaccggcctc gcggtcttcg cggcctcgcc
     5221 tgcgaggtcg atgcccgcga gcttggctgc ctcacggacc gggtcgtaga acttcgagtc
     5281 gacgacctgc atgccgtccc agctgtagag gtccttgaag gctttcttgc ccgcatcagt
     5341 ctgcgagtag tcggccatcg ccttctggat ctgcgtcatg aggtcggccg ggaatccctt
     5401 gcggacggcc gcgccgtcgt tcgggatcgg gcccgccgtg tcgatcacct tcgtcttctg
     5461 catgatgtcc gccggcatgc cggctgacgt gcggacgtcg atgaacgtgg cggcgccgtc
     5521 gaccgcgccc tggtagacct gcagcgcggc cttgtcgtgg ccgcccgcga agatgatgcc
     5581 gttcgcggcg aagaaggtct tcgggtcctg gctcgtcttc tccttgatct tcagcgaggg
     5641 gaagagatac ccggacgccg acagggtgtc gacgaacgcg aacttcttgc ccttgagccc
     5701 gttgatgtcg ttgatgccgc tgtccgagcg gacgaggatc tgcgacttgt acgtcgtgga
     5761 cggcttaccg gtgtcgtcgt tgcggagcgc gccgaggaac ggcgtcgcgc agccacgatc
     5821 gagcgcgaga gccatctgca gcggtgcgag gaaggccacg tcgacggagc ccgcgcacat
     5881 gccctcgatc gtcgcggcgt agctcgtcgg caccgagacc ttccacttga gcgatgtggc
     5941 ccgctcgagc gacgcggtga tcgcgttgcc gctcgccgtc acgcgctggg tgtcctgcga
     6001 gggagtgaat gaaaggacga tgggccgatc gaacgaccca aggcgcggcc cgctcggggc
     6061 cgcggatgcg gcgggtgcgg acgccgtcgg tgtggtgctc gtgggtgtgc caccgcatgc
     6121 cgcgatgacc agtcccgcgg ccaacgccca cccgagtggg ccgcgcaggc tgagacgcat
     6181 gggtaccctc cttcgaactc tggaagcgcc cgtatcatag gactctgatg tcgttcctcg
     6241 acaagctcgc gaccgcgacg cgagcgcggg acagcatcgt gtgcgtcggc ctcgaccccg
     6301 aacccgccct catccctgaa gcgctcggcc gtgggccgca ggccgcgctc cgcttcctgc
     6361 gacgcatcgt gcgcgcgacc gcggagtacg tgtgctgtta caaaccgaac ctcgcgttct
     6421 acgagcgcta cggcggcgcg gcgtacgaca tcctcggcct gaccctacag gccatccccg
     6481 acgacatccc cgtcatcctc gacgcgaagc gcggcgacgt accgaacacg tcggaggcgt
     6541 acgcggatgc gctgttcgac gccttccacg ccgatgcggc gacggtcgcg ccctacctcg
     6601 gcatggattc gatcgcgccg ttcgcccgcg acgaccgctt cgcgctcgtc ctcgcgcgca
     6661 ccagcaatcc cggggcgcgc gacctccagg acttgcggat cgacgaccgt ccgctgtacg
     6721 agcatgtggt ggatgcctgc gtgcgtgcct tcccgcgcga ccgtgccggc ttcgtcgtcg
     6781 gcgcgacgta tccggaggag gcgcgccgcc ttcgcgagct cgcccccgat cgcctgttcc
     6841 tcatgcccgg cgtcggcgcg cagggcggcg acgtggccac ggcgttgcgc gcggcgctgg
     6901 acgcgagcgg cggcggcgtg cttcccgcgg catcgcgctc ggtgatcttc gcgtcgcgcg
     6961 gtgcggacta cgagcgggcg gccgcggacg ccgcgcgcgc cctccgggac gccgcgaacg
     7021 cgggacgggc gctcgcgatg gaacgtcgct gatgttcggc atcggtcccg aggagctcat
     7081 gatcgtcgca ctcatcgccc tgctcgtgct cggcccggag cgcatgccga agctcatgcg
     7141 cgacatgggg aaggtcctgg gagacctccg gaagacatcc gacgagctgc gcgacgactt
     7201 catgaacgcc gacaagttcc tcgaggcgaa ggccgcggcg cccgcgaagc ccgagccggc
     7261 gccggccgag agcgtgccga gcggtccacc gggcgagtcg gccttcgacc gcgagatgcg
     7321 ccaggcgaag gagcggctcg cgaaaccgga agcctgacgc acctatactg ctgacatcga
     7381 gcgcccacgg ggcgctcgtt ttcacgctcg gagggggagc tcgccgttgg gtcgcctggg
     7441 tccatggctc atcgccatca tcgcggtgct cgcgatcttc atcgacatcc cgaagcacca
     7501 gttctcgttc cccttcaact gcccgggcat ctgcctgaag atcggcggct tccaggtcaa
     7561 ccaggagatc aagacgcacc tgggcctcga cctccagggc gggacgcagc tgatcctgcg
     7621 catgcagctg gagaagctgc cgccgggcgt gaccacgccg cagagcgagc tccagacgca
     7681 ggccatccgt gtcatcgacc gccgcatcaa cagcctcggc gtgtcggagc cggtcatcca
     7741 gggcctcggg gacgacaagg tcctcgtgca gctccccggc gtagacgacc tgcagcaggc
     7801 gaacgacatc gtgacccggc aggcgttcct cgagatcaaa gtgcccgaca aggacaagcc
     7861 ggggtcgttc aagtcgctcg agccgccgtt caccggcgcg aacctcaagc cgacgttcgt
     7921 cacgttccag aacgggacga agccggtcgt gaacttcgaa ttcggctcgc ccgacgacca
     7981 gcgctgggtg cagctgtcga aggactactt cgaccagccc gtccagatca cgctcgacac
     8041 caaggagatc tccgccccga cgatcagagc gatcttcacg gacgggcggg ggatcatcga
     8101 gggcaacttc accacgcagt cggccaagga gctctcgctc ctgctcaact ccggcgcgct
     8161 gccggtgccg ctcgaggtca tccagtcctc gcgcgtggaa ccgacgctcg gtcaggagtc
     8221 cgtgcagcgc tcgctcgtcg cgggcgcgat cgggctgctg ctcgtcgcgt tcttcatgat
     8281 cgcgtactac cgcctgcccg gcgcgatcgc ggtgatcgcg cttctctact acacggcgct
     8341 cacgtatgcg ctcttccgcc tggtcccggt caccctgacg ctcgccggcg tcgcgggctt
     8401 catcctctcg atcggcatgg ccgtagacgc gaacgtgctc acgttcgagc gtctcaagga
     8461 ggagctgcgc tccggcaagt cgctgcgcgc cgcggtcgag gccgggcgcg ttcgcgcgtt
     8521 cccatcgatc ctctactcga acctcgcgac gatcctgacc gccggcatcc tgttctggtt
     8581 cggcaccgga acggtgaagg gcttcgcgct cacgctggcc atcggtgtcg cggtgtcgtt
     8641 cttcaccgcg gtgatcgtca cgcagatgct gctgcacgcg gcgatcgaga tcccgtcatt
     8701 gcgctcgcgc cggctgtacg gcgtcgagga gcggcagagc gaaggccgcg agggccgcgt
     8761 ggccccggtg gtgagctgat gctgcacttc gtcgaacgca agtggatgta cttcctcctc
     8821 tcgggcctgc tcatcgtccc gggtgtcgtg ttcctgctgc tcggcgggct gaggcccggc
     8881 atcgagttca agggcggcac gctcctcgac gccacattcc cgggcgcgac gcccgccgtg
     8941 agcgacgtac gcgaggtgat gtccacgctc gggcgtggcg aggctgtggt ccaggccgcg
     9001 gagggcgggc gcatcgagat ccgcaccttc gagatgtcgc ccgaggacgt gacgaaggcg
     9061 acgaaggcgc tgcaggacaa gttcggagcg gg
//
LOCUS       DASTZR010000239         2637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1136052, whole genome shotgun sequence.
ACCESSION   DASTZR010000239 DASTZR010000000
VERSION     DASTZR010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136052"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="VFV20_05355"
     CDS             <1..986
                     /locus_tag="VFV20_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEU5287810.1"
                     /translation="FDHLRHTLTVLAHDDAAHPGDAARAIERVFTALDAPLPQLAAPS
                     HQKADVRANGTPRDYEQSVRRAVDLIRGGDCIQIVLAQRFDVRPAPDALALYRALRHV
                     NPSPYMFLLETAAATLVGASPEPFVRVEGGKVVMHPIAGTRPRGADEGEDAANEIDLR
                     RSEKERAEHVMLVDLARNDIGRVSRPGTVRVKELMRVDRFSHVMHLTSVVEGDLRADL
                     DALDAFRACFPAGTVSGAPKIRAMERIAELETDQRGPYAGAVGYLGFDGALDTCITIR
                     TATIAKGVCRIEAGAGIVADSDPAAEEAETRAKARALLRAVELATDGSLLR"
     gene            983..1558
                     /locus_tag="VFV20_05360"
     CDS             983..1558
                     /locus_tag="VFV20_05360"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEU5287811.1"
                     /translation="MSKILLVDNYDSFTFNLAQALSSLGHTVEVLRADDPALDAALDA
                     KPDRVVISPGPGRPEEAGRSPEIVREAPKRGIPTLGVCLGLQCIAIAFGARVVRAPEP
                     RHGKTSLIQHDGRGVFAGVPVPTEATRYHSLMVDEATLPDELVVTARAEDGVVMGLRH
                     RTLPLEAVQFHPESVLSADGLRMLENFSGRS"
     gene            1545..2579
                     /gene="trpD"
                     /locus_tag="VFV20_05365"
     CDS             1545..2579
                     /gene="trpD"
                     /locus_tag="VFV20_05365"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU5287812.1"
                     /translation="MDVREALGVVTSGRALTRAEAEAAMASVMAGEATPAQLGALLAA
                     LNIRGETADEIAGFASAMRAHAVRVDVGDGAIDVVGTGGDRSNSINISTLSGLVAAGA
                     GALVAKHGGRAASSACGSADVLEALGVKIDLGPDGVAACVKDAGIGFMFAPRYHPAMR
                     HAAPVRRELGIRTVFNLLGPISNPAGVRRYLLGVPSAALGETMARALAELDVAHALVV
                     HGSDGLDEISPSAETAAWEVRGREVRALTIVPEDAGVGRVRREEIVGGDPGTNARIAH
                     EVLNGAKGGARTAVVLNAAAACYVAGLAGSIRDGAAVATRSIDSGAALQALERFVATS
                     QQLGAAEVQA"
BASE COUNT          360 a          949 c          994 g          334 t
ORIGIN      
        1 cgttcgacca tctgcggcac acgctcaccg tcctcgccca cgatgacgcg gcgcacccgg
       61 gtgacgcggc ccgcgcgatc gagcgcgtct tcacggcgct ggacgcgccg ctgccgcagc
      121 tcgcggcgcc gtcgcaccag aaggcggacg tgcgtgcgaa tggcacgcca agggactacg
      181 agcagagcgt gcggcgggcg gtcgacctca tacgtggcgg cgactgcatc cagatcgtcc
      241 tggcccagcg cttcgacgtg cggcccgcac ccgatgcgct cgcgctgtac cgcgcgctcc
      301 gccacgtgaa cccgtcgccg tacatgttcc tgctcgagac cgccgccgcg accctagtgg
      361 gggcgtcgcc cgagccgttc gtccgcgtcg agggcggcaa ggtggtgatg cacccgatcg
      421 ccgggacgcg cccgcgcggc gcggacgagg gcgaggacgc ggcgaacgag atcgacttgc
      481 gccgctcgga gaaggagcgc gccgagcacg tcatgctcgt ggacctcgca cgcaacgaca
      541 tcggccgcgt gtcgcgtccg gggacggtgc gcgtcaagga gctcatgcgc gtcgaccgct
      601 tctcgcacgt gatgcacctc acctccgtgg tcgagggcga cctgcgcgcg gacctcgacg
      661 ccctcgacgc gttccgcgcc tgcttcccgg ccggcacggt gtccggcgcg cccaagatcc
      721 gcgcgatgga gcgcatcgcg gagctcgaga ccgatcagcg cggaccgtac gccggcgcgg
      781 tcgggtacct cggcttcgac ggcgcgctcg acacctgcat caccatccgc accgcgacga
      841 tcgcgaaagg tgtgtgccgc atcgaggcgg gcgcgggcat cgtggccgac tccgatcccg
      901 ccgccgagga ggccgagacg cgcgcgaagg cgcgcgcgct ccttcgcgcc gtcgagctcg
      961 cgaccgacgg gagcctgctg cgatgagcaa gatcctcctc gtcgacaact acgactcgtt
     1021 caccttcaac ctggcgcagg cgctctcgtc gctcggccac accgtggagg tgctgcgtgc
     1081 cgacgacccc gcgctcgacg cggcgctcga cgccaagccc gaccgggtcg tgatctcgcc
     1141 cggcccgggg cggccggaag aggctggccg ctcgcccgag atcgtgcgcg aggcgccgaa
     1201 gcgcgggatc ccgacgctcg gcgtctgcct cggcctgcag tgcatcgcga tcgcattcgg
     1261 cgcccgggtg gtccgggcgc cagagccgcg ccacgggaag acgagcctga tccagcacga
     1321 cggcaggggc gtgttcgcgg gcgtgcccgt accgaccgag gcgacgcgct accactcgct
     1381 gatggtcgac gaggccacgc tgcccgacga gctggtggtg acggcgcgcg ccgaggatgg
     1441 cgtggtgatg ggcctccgcc accgcacgct tccgctggag gctgtgcagt tccacccgga
     1501 gagcgtgctg agcgcggacg gactgcgcat gctggagaac ttcagtggac gttcgtgagg
     1561 ccctcggcgt cgtgaccagc ggacgcgcgc tcacgcgcgc ggaggccgag gcggccatgg
     1621 cgtcggtgat ggcgggcgag gccacgccgg cgcagctcgg cgcgctcctc gcggcgctca
     1681 acatccgcgg cgagaccgcc gacgagatcg ccggcttcgc gtcggcgatg cgcgcccacg
     1741 cggtgcgcgt ggacgtcggc gacggcgcga tcgacgtcgt cggcaccggc ggcgaccgct
     1801 cgaacagcat caacatctcg acgctctcgg ggctcgtggc cgccggcgcg ggggcgctcg
     1861 tcgccaagca tggcggacgc gccgcctcga gcgcgtgcgg cagcgcggac gtgctcgagg
     1921 cgctcggcgt gaagatcgac ctcggaccgg acggcgtggc cgcgtgcgtg aaggacgccg
     1981 ggatcggctt catgttcgcg ccgcgctacc acccggcgat gcgacacgcc gcgccggtcc
     2041 ggcgcgagct cggcatccgg acggtcttca acctgctcgg accgatctcg aatcccgcgg
     2101 gcgtgcggcg gtacctgctc ggcgtaccaa gcgccgcgct cggcgagacg atggcgcggg
     2161 cgctcgccga gctcgacgtc gcgcacgcgc tcgtcgtcca cgggagcgac gggctcgacg
     2221 agatcagccc gtcggcggag acggcggcgt gggaggtgcg tggccgcgag gtccgcgcgc
     2281 tcacgatcgt tcccgaggac gcgggggtgg ggcgcgtgcg acgtgaggag atcgtcggcg
     2341 gcgatcccgg gacgaacgcc cggatcgcgc acgaggtcct gaacggcgcg aagggcggcg
     2401 cgcggacggc cgtggtgctc aacgccgcgg ccgcgtgcta cgtcgcgggc cttgccggga
     2461 gcatccgcga cggcgccgcc gtcgcgacgc ggtcgatcga ctcgggcgcg gcgctgcagg
     2521 cgctcgagcg attcgtcgcg acgagccagc agctcggcgc ggcggaggta caggcgtgac
     2581 ggacttcctc gacgacgtcg tgcgacaccg gcgggccgac gtcatggccg cgagcga
//
LOCUS       DASTZR010000240         3552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_744680, whole genome shotgun sequence.
ACCESSION   DASTZR010000240 DASTZR010000000
VERSION     DASTZR010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744680"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VFV20_05370"
     CDS             <1..924
                     /locus_tag="VFV20_05370"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEU5287813.1"
                     /translation="VLPAEAAARKVDDAVRRDFPPSRTSPIYLAVDGASTNEIGAYAQ
                     RLARVRGVAAVAPPQRVSPGAWRVDVYPSADFLAAEKGGLVGRLRATPVDARVLAGGL
                     APWFHDQKASMRAHLPAALVVVAVATLLILFLMTGSVILPVKALLMNLLTVSVAFGAV
                     VWIFQDGRLESVLDFESRGGLDFSQPILLFAVVFGLSTDYGVFLLMRIREEYLRTGDN
                     EESVARGLERTGRIVTAAALLLCVAVGTFATGRLVFVKELGVGIAVGVLVDATIVRAL
                     LVPSLMKLLGDWNWWAPEPLRRIHRRLGLDH"
     gene            complement(921..1313)
                     /locus_tag="VFV20_05375"
     CDS             complement(921..1313)
                     /locus_tag="VFV20_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEU5287814.1"
                     /translation="MRELSIRTERRTQLLDITREVEGAVDGEGSAVLVYVPHTTAGVT
                     INEHADPDVARDFEAALERMVGDDWGWRHIEEGEENAPSHIRAALMGPQVLIPLRDGK
                     LALGTWQGIFFCELDGPRTRSVYVTVLR"
     gene            1334..2830
                     /locus_tag="VFV20_05380"
     CDS             1334..2830
                     /locus_tag="VFV20_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287815.1"
                     /translation="MPPAAPSDRELDAYRDQADRFIAELDEEFYLHYAGLKDDFDIVS
                     IYERHANLTTLDQANSIALAVNGGSRVRELWRFACEGYLGNLTRDQAQRIARTEAELS
                     TTVDGEPLPFRMLRPTIANEADRPKRLELERRRQELTAEHLNPLHLQAAREQIDAVHA
                     LGSPTYRSLYERFGYDLDGLADQCRQLLASTERLWETWGDRLFRERTGVGLDEAGPAD
                     VPRVFRAEAWDTAFPGERMIPALEATLGDLGVDLRSQENVILDVEQRPKKDPRAFCAP
                     IEVPDKVMLVIQPQGGADDWRALFHEAGHAEHFGHTSRDLAMEEKRLGDNAVTEGWAM
                     LLEHLTSDPAWLTRRLDFPRPDEYAREGAVNLLYFVRRYSAKLLYELEFHATPADEIE
                     SMRPRYVEILGDALKIDIASDNYLSDIDSGYYVTSYLRSWAFEAQLRGYLREKFGNAW
                     FTSRDAGGLIRELWSEGQRLNADEMLKDVTGATLTMEAVGERISEVLS"
     gene            2857..>3552
                     /locus_tag="VFV20_05385"
     CDS             2857..>3552
                     /locus_tag="VFV20_05385"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5287816.1"
                     /translation="MDIPETRYARSGDVNVAYQVVGDGPFDLVYVPPFISNVELVWDV
                     PWSGPGLRALSSLCRLIMFDKRGTGLSDRVSAAPSLEERMDDVRAVMDAAAVERAAVV
                     GVSEGGPMCALFAATHPDRTRALVTMGSYARRNWAPDYPIGRKPEQDTWLRPTAEQWG
                     EYAARRFLAERAPSVADDPEAIDWYTSYLVRCASPSAVAAITDMNEEIDVRPVLPTVR
                     VPALVLHRQEEYLR"
BASE COUNT          485 a         1287 c         1272 g          508 t
ORIGIN      
        1 gtcctgcccg cggaggccgc ggcgcggaag gtcgacgacg cggtccgccg cgacttcccg
       61 cccagccgca cgagcccgat ctacctcgcc gtcgacggcg cgtcgacgaa cgagatcggg
      121 gcgtacgcgc agcggctcgc acgcgtgcgc ggcgtcgccg cagtcgcgcc gccgcagcgc
      181 gtctcgcccg gcgcatggcg tgtggacgtc tacccgagcg ccgacttcct cgccgccgag
      241 aagggcgggc tcgtcggccg cctgcgcgcg acgccggtgg acgcgcgcgt tctggccggc
      301 gggctcgcgc cgtggttcca cgaccagaag gcgtcgatgc gcgcgcacct gcccgcggcg
      361 ctagtcgtgg tcgcggtcgc gacgctgctc atcctcttcc tgatgaccgg ctcggtgatc
      421 ctgccggtga aggcgctgct gatgaacctg ctgacggtga gcgtggcgtt cggcgccgtc
      481 gtgtggatct tccaggacgg caggctggag tccgtgctcg acttcgagag ccgcggcggg
      541 ctcgacttct cgcagccgat cctcctcttc gccgtcgtgt tcggcctctc gaccgactac
      601 ggcgtgttcc tgctgatgcg catccgggag gagtacctgc gcacgggcga caacgaggag
      661 tcggtcgcgc gcgggctcga gcgcaccggg cggatcgtga ccgccgccgc gctcctcctc
      721 tgcgtcgccg tcggcacgtt cgccaccggg cggctcgtct tcgtgaagga gctcggcgtc
      781 gggatcgcgg tcggcgtcct cgtcgacgcg acgatcgtgc gcgcgctgct cgtgccgtcg
      841 ctgatgaagc tcctcggcga ctggaactgg tgggcgcccg agccgctacg ccggatccac
      901 cgccgcctcg gcctcgacca ctagcggagc accgtgacgt acaccgaccg cgtgcgcggg
      961 ccgtcgagct cgcagaagaa gatgccctgc cacgtgccga gcgcgagctt cccgtccctg
     1021 agcgggatca gcacctgcgg ccccatcagc gccgcgcgga tgtgcgaggg ggcgttctcc
     1081 tcgccctcct cgatgtgccg ccagccccag tcgtccccga ccatgcgctc gagcgccgcc
     1141 tcgaagtcgc gcgcgacgtc cgggtcggcg tgctcgttga tcgtcacgcc ggcggtcgtg
     1201 tgcggcacgt agacgaggac cgccgacccc tcgccgtcga ccgctccctc gacctcgcgc
     1261 gtgatgtcga gcagctgcgt ccggcgctcg gtcctgatcg agagctcgcg cacgggtctg
     1321 taggctaacc cgcgtgccgc ccgccgcgcc ttccgaccgt gagctggacg cgtaccgcga
     1381 ccaggccgac cggttcatcg ccgagctcga cgaggagttc tacctccact acgcgggcct
     1441 gaaggacgac ttcgacatcg tctcgatcta cgagcggcac gcgaatctga cgacgctcga
     1501 ccaggccaac tcgatcgccc tggcggtgaa cgggggctcc cgcgtccgcg agctgtggcg
     1561 cttcgcgtgc gagggctacc tcgggaacct gacccgcgac caggcgcagc ggatcgcgcg
     1621 cacggaggcg gagctctcga ccacggtcga cggggagccg ctcccgttcc ggatgctgcg
     1681 gccgacgatc gcgaacgagg ccgaccggcc gaagcggctc gagctcgagc gccgccggca
     1741 ggagctgacg gcggagcacc tgaacccgct gcacctccag gccgcgcgcg agcagatcga
     1801 cgccgtgcac gcgctcggca gccccaccta ccgctcgctc tacgagcgct tcggctacga
     1861 cctcgacggc ctcgccgacc agtgccggca gctgctcgcg tccacggagc gcctgtggga
     1921 gacctggggc gaccgcctct tccgcgagcg cacgggcgtc ggcctcgacg aggcgggccc
     1981 ggcggacgtg ccgcgcgtct tccgcgccga ggcgtgggac accgcgttcc ccggcgagcg
     2041 gatgatcccc gcgctcgagg cgacgctggg cgacctcggc gtcgacctgc gctcgcagga
     2101 gaacgtcatc ctcgacgtcg agcagcgccc gaagaaggac ccgcgcgcgt tctgcgcgcc
     2161 gatcgaggtg cccgacaagg tgatgctcgt catccagccg cagggcggcg cggacgactg
     2221 gcgggcgctc ttccacgagg ccggccacgc cgagcacttc ggccacacga gccgcgacct
     2281 cgcgatggag gagaagcgcc tcggcgacaa cgccgtcacc gagggctggg cgatgctgct
     2341 cgagcacctg acgagcgacc cggcgtggct cacgcgccgg ctcgacttcc cgcgccccga
     2401 cgagtacgcg cgcgagggcg ccgtcaacct cctctacttc gtccgccgct acagcgcgaa
     2461 gctcctgtac gagctggagt tccacgcgac ccccgcggac gagatcgagt cgatgcgccc
     2521 gcgctacgtc gagattctcg gcgacgcgct gaagatcgac atcgcgagcg acaactacct
     2581 ctccgacatc gactcgggct actacgtgac gtcgtacctg cgctcgtggg cgttcgaggc
     2641 gcagctgcgc ggctacctgc gcgagaagtt cggcaacgcg tggttcacgt cgcgcgacgc
     2701 gggcgggctg atccgcgagc tgtggagcga gggacagcgc ctgaacgcgg acgagatgct
     2761 gaaggacgtc accggcgcca cgctcacgat ggaagccgtc ggggagcgca tctccgaagt
     2821 cttgtcgtag ccccgccgcg ggcgcagact agcgacgtgg acatccccga gacgcgctac
     2881 gcccggagcg gcgacgtcaa cgtcgcgtac caggtggtcg gcgacggccc gttcgacctc
     2941 gtctacgtgc cgccgttcat ctcgaacgtc gagctcgtct gggacgtgcc ctggagcggc
     3001 cccggcctgc gcgccctctc ctcgctctgc cggctgatca tgttcgacaa gcgcggcacg
     3061 ggcctctccg accgcgtctc ggcagccccg tcgctcgagg agcggatgga cgacgtgcgc
     3121 gcggtgatgg acgcggcggc ggtcgagcgg gccgcggtgg tcggggtgtc cgagggcggg
     3181 ccgatgtgcg cgctgttcgc cgcgacgcat ccggaccgca cgcgggcgct ggtgacgatg
     3241 gggtcctacg cgcggcgcaa ctgggcgccg gactatccga tcgggcgcaa gccggagcag
     3301 gacacgtggc tgcggccgac cgcggagcag tggggcgagt acgcggcgcg gcggttcctg
     3361 gccgagcggg cgccgtcggt cgcggacgat ccggaggcga tcgactggta cacgtcatat
     3421 ctggtccgct gcgcgagccc gtcggccgtg gcggcgatca ccgacatgaa cgaggagatc
     3481 gacgtgcggc cggtgctgcc gaccgtgcgg gtgccggcgc tcgtcctgca ccggcaggag
     3541 gagtatctgc gc
//
LOCUS       DASTZR010000241         4179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_639975, whole genome shotgun sequence.
ACCESSION   DASTZR010000241 DASTZR010000000
VERSION     DASTZR010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639975"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VFV20_05390"
     CDS             complement(<1..908)
                     /locus_tag="VFV20_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEU5287817.1"
                     /translation="MRQDDLVARVRANVAVGLDPGRMLEAYADPARRRTIHERVRYAA
                     GPDVADEEVAELSAALFGFGVLQSLLDDPKVTDVLVNAPDEIYVERDGVLERAPMRFS
                     SAAEVAELAHRIAAGVGRELTLERPYVDARMRDGSRANAVIAPVGGPTISVRKFSRLS
                     IPLRGPSPSWLAEGALTAEVADLLAAAVRGRANLLIVGATGAGKSTLLRSLAAEIPPT
                     ERLIVVEDTNELVLPHPHVVHLEAMSGRESGVGIADLVANSLRMRPDRIIVGEVRTPR
                     EASALLDALTTGHDGSLTTAHAGSARA"
     gene            complement(905..1687)
                     /locus_tag="VFV20_05395"
     CDS             complement(905..1687)
                     /locus_tag="VFV20_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287818.1"
                     /translation="MGAAVSRVRAWLDATPLASIGLALMREATGRSEPLEAIIPVRPA
                     RLEADVRVGLWSLCGGVGTSTIAALVAQRSSAGGHAPLLMDLDRWAPSLALRARIEAA
                     TIVDVLVQPDRERELVSRWADVPFLPGSPRLHGDFDGERIAALARRLAHGRALVLDLG
                     CGPEALDASVLACLTHLCVVSGGRASQLQATFCARPLLRAVTCRVGLVAVGVEHDDAA
                     LIAGRVSLPLLAGVPHDEFLARDEFAARAPTMRAIDALIRSL"
     gene            complement(1672..2226)
                     /locus_tag="VFV20_05400"
     CDS             complement(1672..2226)
                     /locus_tag="VFV20_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287819.1"
                     /translation="MVARRVQVRSVGDVDRHVARLPWVRFTRRHDGLFLECDRDEVGR
                     VTVLLARAGLALEPAETLPQPPSLVAAVVTHLAPFGRDDIVDVVTISAASLGDSTAWA
                     RRRRRPSLLGRNGAWRRSTRALLHGDDVAFHWCRVVFAGPGLLRERALAGARPVVFDR
                     DATIRRPQELALAGNGALAAWVRP"
     gene            complement(2232..2873)
                     /locus_tag="VFV20_05405"
     CDS             complement(2232..2873)
                     /locus_tag="VFV20_05405"
                     /inference="COORDINATES: protein motif:HMM:NF028286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcpC/CpaB family pilus assembly protein"
                     /protein_id="HEU5287820.1"
                     /translation="MIPRRWLVALAVASGLVSGVVYEVSMQREDVVVVVRDVDVPRPL
                     VESDVEVRSVSVGLVPDDAVRRREDVVGLIPRAPLLRGQIVVARAVSDELATFSSGLT
                     VPSGLHAVAIPVSAVNAVGGAIAPGVHVDVLAIPILGRAPAGRTSELLASAVIVLDVR
                     GESGAAFVRRDASVTVMSDRIASVVIAVAPADQIRVADRIATSTFVLALARGR"
     gene            complement(2870..3784)
                     /locus_tag="VFV20_05410"
     CDS             complement(2870..3784)
                     /locus_tag="VFV20_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287821.1"
                     /translation="MFIATLVVVLVALPAAPAHAWDPLGDEFDAGPGDPDPMPFVVPE
                     GLYAVTDVYAGDVVTTSGGTTTYTTETIHDTPGTFARVVDVVDSGAGSRFDGASFNGR
                     AALPDGRPVAGIYYEDFVLTASGFVSVNIVFFQDDSAFRAPAPTAQPPAATLPPSTTP
                     SPVAPVAAVAVPRASATPAPTARPTPEPEPARPIVATAGVALGPDAPVLTSIEILRGR
                     TVRLWPRAFVGGAPVATRSWRLVSGAADVMTRTAGGPGDACDATWLAPTLSAGAAVLR
                     FEITTDAAPGRVLTATIAVAVRSPALMQ"
     gene            complement(3790..>4179)
                     /locus_tag="VFV20_05415"
     CDS             complement(3790..>4179)
                     /locus_tag="VFV20_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287822.1"
                     /translation="GALRVLAARVASAADLATLAAVDDQDEMVLVRDGTLRLASDATE
                     ITRRFFALELEQIAAHLERSPGAVAAEADVAAFATGLAFDPITGMRYDRPTIRLDAAV
                     GVRTPAFGALLLPVITTVHVRSVSSPR"
BASE COUNT          658 a         1488 c         1554 g          479 t
ORIGIN      
        1 gcgcgcgccg agccggcgtg ggccgtggtc agcgagccgt catggccggt cgtgagcgcg
       61 tcgagcaatg ccgacgcttc gcgcggcgtg cggacctccc cgacgatgat ccggtcgggg
      121 cgcatccgaa gcgaattcgc gaccagatcg gcgatgccca caccggactc gcggccgctc
      181 atcgcttcga ggtggacgac atgcggatgc ggcaacacga gctcattggt gtcctcgacc
      241 acgatgaggc gctcggtcgg agggatctcc gcggcgagcg atcgcaacag cgtgctcttc
      301 ccggcgccgg tcgcgccgac gatgaggagg ttcgcgcggc ctcgtaccgc ggccgcgagc
      361 agatcggcga cctcggcggt gagcgcgccc tccgcgagcc acgaggggct cggaccgcgc
      421 agcgggatcg acaggcgcga gaacttgcgg accgagatcg tcggtccgcc gaccggtgcg
      481 atgacggcgt tcgcgcggct gccgtcgcgc atgcgtgcgt cgacgtaggg ccgctcgagc
      541 gtgagctcgc gcccgacgcc ggccgcgatg cgatgggcga gctcggcgac ctcggcggcc
      601 gaggagaagc gcatcggcgc ccgctcgagc acgccgtcgc gctcgacgta gatctcgtcg
      661 ggcgcgttca ccagcacgtc ggtgaccttg ggatcgtcca gaagggattg gagcacgccg
      721 aatccgaaca gcgccgccga gagctcggcg acctcctcgt cggcgacgtc cggtccggcg
      781 gcgtagcgga cgcgctcgtg gatcgtccga cggcgcgcgg gatccgcgta cgcctcgagc
      841 atgcgcccgg ggtccaggcc gacggcgacg ttcgcacgaa cgcgcgcgac gagatcgtcc
      901 tgcctcacag ggagcggatg agcgcgtcga tcgcccgcat cgtcggcgcg cgcgccgcga
      961 actcgtcccg tgcgaggaat tcgtcgtggg gcacaccggc gagcagtggg agtgagacgc
     1021 gccccgcgat cagcgcggcg tcgtcgtgct ccacgccgac ggccacgaga ccgacgcgac
     1081 acgtgaccgc ccgcagcaac gggcgcgcgc agaacgtcgc ctggagctgt gaggcgcgtc
     1141 cgccgctgac cacacagaga tgcgtgagac acgcgaggac cgacgcgtcg agcgcctccg
     1201 gaccgcatcc gaggtcgagc acgagcgcgc gaccgtgcgc caggcgccgc gccagtgccg
     1261 cgatgcgctc gccgtcgaag tcgccgtgca ggcgtggaga gcccggaagg aacgggacgt
     1321 ccgcccagcg tgacaccagc tcgcgctcgc ggtcgggctg cacgagaacg tccacgatcg
     1381 tcgccgcctc gatgcgtgcg cgcagcgcga gggacggtgc ccagcggtcg agatccatca
     1441 ggagcggagc gtggccgccg gcactcgagc gctgcgcgac gagcgccgcg atcgtgctcg
     1501 ttccgacgcc gccacacagc gaccagagcc cgacgcgcac gtccgcctcg agccgcgccg
     1561 gccgcaccgg gatgatcgcc tcgagcggct cggatcgtcc cgtcgcttcc cgcatgagcg
     1621 cgagcccgat cgatgcgagc ggcgtggcgt caagccaggc gcgcacgcgg ctcacggccg
     1681 cacccatgcg gcgagggcac cgttgccggc gagagcgagc tcctgcggcc gacggatggt
     1741 ggcatcccga tcgaagacga cgggccgcgc gccagcgagc gcgcgctcgc gcaggagtcc
     1801 gggaccggcg aagaccacac ggcaccagtg gaacgcgacg tcgtcgccat gcaggagcgc
     1861 ccgcgtggac ctgcgccacg cgccgttccg gccgaggaga gaggggcgcc gccggcgccg
     1921 cgcccacgcg gtcgaatcgc cgagcgacgc cgcgctgatg gtgacgacgt cgacgatgtc
     1981 gtcccggccg aatggcgcga gatgcgtgac caccgccgcc acgagggagg gaggctgcgg
     2041 cagcgtctcc gcgggctcga gcgcgagacc cgcgcgcgcc aggagcacgg tgacccggcc
     2101 gacctcgtcg cggtcgcact cgaggaagag accgtcatga cggcgtgtga accgcaccca
     2161 cggcaggcgg gcgacgtgcc ggtccacatc gccgacggag cgcacctgca cgcgtcgtgc
     2221 gaccaccgca ctcaccggcc gcgcgccagc gcgagcacga acgtgctcgt cgcgatgcgg
     2281 tcggcgacgc ggatctggtc cgcgggcgcg acggcgatga cgacgctcgc gatgcgatcg
     2341 ctcatcaccg tcactgaagc gtcgcgtcgc acgaacgcgg cgccgctctc accgcgcacg
     2401 tcgagcacga tcaccgccga cgcgagcagc tcggacgtcc ttcctgccgg cgcgcgaccc
     2461 agtatcggaa tggccaggac gtcaacgtgc acgcctggcg cgatggcgcc gcccacggcg
     2521 ttcaccgctg agaccgggat ggccacggcg tggaggccgg acgggacggt gaggcccgag
     2581 ctgaacgtgg cgagctcgtc ggagaccgcg cgcgcgacga cgatctgacc gcgcagcaat
     2641 ggggctcgcg gtatgaggcc gacgacgtcc tcgcgccgac ggaccgcatc gtcggggacg
     2701 aggccgaccg atacgcttcg gacttcgacg tcagactcca cgagcggccg tggaacgtcg
     2761 acatcacgca cgacgacgac gacatcctcg cgttgcatgg agacctcgta gacgacgccg
     2821 gagacaagac ccgacgccac cgcgagcgcg acgagccagc ggcgtgggat cactgcatca
     2881 gggcgggcga ccgcacggcg acggcgatcg tcgccgtcag gacgcgcccg ggcgcggcgt
     2941 cggtcgtgat ctcgaagcgc aggacggccg ccccggccga cagtgtcggt gcgagccacg
     3001 tggcgtcaca ggcatcgccc ggcccgcccg cggtgcgcgt catcacgtcg gccgcgcccg
     3061 agacgagacg ccacgaccgc gtcgcgacgg gcgcgccgcc gacgaaggcg cgcggccaca
     3121 ggcgcaccgt gcgtccacga aggatctcga tcgacgtgag gaccggtgca tccggaccca
     3181 gcgcgacacc cgcggtcgcc acgatcgggc gcgccggctc gggctctggt gtgggacgag
     3241 ccgtcggagc gggtgtggct gacgcgcgcg gcacggcgac ggccgctacc ggtgcgaccg
     3301 gcgacggcgt cgtcgacggg ggcagcgtcg cggccggcgg ctgtgcggtc ggcgcaggcg
     3361 cgcggaacgc gctgtcgtcc tggaagaaca cgatgttgac cgacacgaag ccgctcgcgg
     3421 tgagcacgaa gtcctcgtag tagatgccgg cgaccggccg cccgtccgga agggccgccc
     3481 gtccgttgaa cgatgcgccg tcgaaccggc ttcccgcgcc ggagtccacg acgtcgacga
     3541 cccgcgcgaa ggtcccaggg gtgtcatgga tcgtctcggt cgtgtacgtc gtcgtgccgc
     3601 cggatgtcgt cacgacgtcg ccggcataca cgtccgtgac ggcgtaaagg ccctccggga
     3661 cgacgaacgg catcgggtcg ggatcgcccg ggccggcgtc gaactcatca ccgagtgggt
     3721 cccaggcatg ggccggcgcc gcgggcaggg ccacgagaac gacgacgagg gtggcgatga
     3781 acatcggtct catcgcggtg agctcacaga tcgcacatgc accgtcgtga tcaccgggag
     3841 cagcagcgcg ccgaacgcgg gcgtgcgaac gcccaccgcg gcgtcgagcc ggatcgtcgg
     3901 ccggtcatac ctcatcccgg tgatggggtc gaacgccagg cccgtcgcga atgccgcgac
     3961 atcggcctcg gcggcgacgg cacccgggct gcgctcgaga tgcgcagcga tctgctcgag
     4021 ctccagcgcg aagaagcgac gcgtgatctc cgtcgcgtcg gacgcgaggc gcaacgtgcc
     4081 gtcgcgcacg agcaccatct cgtcctgatc gtcgacggcg gcgagcgtcg cgaggtcggc
     4141 cgcgctcgcg acgcgcgcgg cgagaacgcg cagcgcgcc
//
LOCUS       DASTZR010000242         2592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_431431, whole genome shotgun sequence.
ACCESSION   DASTZR010000242 DASTZR010000000
VERSION     DASTZR010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431431"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1321
                     /locus_tag="VFV20_05420"
     CDS             161..1321
                     /locus_tag="VFV20_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287823.1"
                     /translation="MKRLLCAVLTAGILAAACTTGTPGTTGPEPASSGSGAPAAKPAI
                     NEQTRGAIQALLQRQHDTMAARDQAAFATTIDPDRLALKRCANESYDIATRQGVGAVA
                     QVGKIEPYLDTYVRAYVQEGSSGQRRLFFRQTADGKWVQTEPKDAELGGEKKTTVGDI
                     QIDYWGVDQDVVEQLGKGAAEAQKIVLANMLSTERRDAFAIRFYPTRGVSGIQGCTVV
                     GFHLTNVATDPYVRFFRYWFNADGSISAPTISFMTHEWLHWAQDQFSPGITARLPWWL
                     VEGWPDYVGKSRGADTIKFVVCQTQTPTLKQLEDGADPYGPPELAPQYYAYANSMVEY
                     LYATYGGADAYRKLALTFKDDANPKTTFPKALSITSDQFYDGWKAAAKKKYC"
     gene            1348..2436
                     /locus_tag="VFV20_05425"
     CDS             1348..2436
                     /locus_tag="VFV20_05425"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEU5287824.1"
                     /translation="MALALVVVACVPTISTGGSPTPAATADIKRSKLDIAYTAFIDLD
                     VHHATSKAALTAMTNALNEQARSSGGKADLPLLEYQDVDEPQLADFKKFADAVAAWAA
                     RNPQLGPDRIADTAIAAMIRVSPDCHTSYLSRTRQVFRSRPDVSSGGSGARVPATGTQ
                     LFGPDSSALQAKLLADGIAYVTFGEWTMHGTYNIVDQLRAALDKAIAAGARAWIFDLR
                     GNPGGNGAETVASWFLNGEAILKTTVKTGNAGTQSANKTLRLPPAYQLPIVVIVNGRS
                     ASASEVFALSLKENGRATIVGATTIGCLGAETPTAMSDGSELDVTVQEYVGAQTGAVY
                     NNKGIPPDVAADDASAVDRAIAILKTKL"
BASE COUNT          447 a          912 c          875 g          358 t
ORIGIN      
        1 tcccgagtgc ccggtctgcg gcgacaaccc gacgctcacc gagtacatcg actacgaggg
       61 cttctgcgcc tcgcccgtgg agtggcaggc ggagcacgcc accaagaacg gccagaaggt
      121 cggcgccgcc gactagcgct cccgtatcct cggtctgcac atgaagcgcc tgctctgcgc
      181 cgtcctcacc gcgggcatcc tcgcggccgc gtgcacgacc ggcacgcccg gcacgaccgg
      241 gcctgagccg gcgtcgagcg ggtccggtgc gcccgcggcg aagccggcga tcaacgagca
      301 gacgcggggc gcgatccagg cgctcctcca gcggcagcac gacaccatgg ccgcgcgcga
      361 ccaggcggcg ttcgcgacga ccatcgaccc ggatcgcctc gcgctgaagc gctgcgcgaa
      421 cgagtcctac gacatcgcga cgcggcaggg cgtgggcgcc gtggcgcagg tgggcaagat
      481 cgagccctac ctcgacacct acgtgcgggc atacgtccag gagggctcga gcgggcagcg
      541 gcgactcttc ttccgccaga ccgcggacgg caagtgggtc cagaccgagc cgaaggacgc
      601 ggagctgggc ggcgagaaga agacgaccgt cggtgacatc cagatcgact actggggcgt
      661 ggaccaggac gtcgtcgagc agctcgggaa gggcgccgcc gaggcgcaga agatcgtgct
      721 cgccaacatg ctctcgaccg aacgccgcga cgcgttcgcg atccgcttct acccgacgcg
      781 gggcgtctcg gggatccagg gttgcacggt cgtgggcttc cacctcacga acgtcgcgac
      841 cgacccctac gtgcgcttct tccgctactg gttcaacgcg gacggctcga tctcggcgcc
      901 gaccatcagc ttcatgaccc acgagtggct gcactgggcg caggatcagt tcagccccgg
      961 catcaccgcg cggctgccgt ggtggctggt cgagggctgg cccgactacg tcggcaagtc
     1021 gcgcggcgcc gacacgatca agttcgtcgt gtgccagacg cagacgccga cgctcaagca
     1081 gctcgaggac ggcgccgatc cgtacggacc gccggagctc gctccccagt actacgcgta
     1141 cgccaactcg atggtcgaat acctctacgc gacgtacggc ggcgccgacg cataccgcaa
     1201 gctcgcgctc acgttcaagg acgacgcgaa tccgaagacg acgttcccca aggcgctgtc
     1261 gatcacgtcc gaccagttct acgacggctg gaaggctgcc gcgaagaaga agtactgctg
     1321 acgacggccc ggctgctccg cgccgcgatg gccctcgcgc tcgtcgtcgt cgcatgcgtc
     1381 cccaccatca gcaccggcgg ctctcccact cccgccgcga ccgcggacat caagcgctcg
     1441 aagctcgata tcgcgtacac ggccttcatc gacctcgacg tccatcacgc cacgagcaag
     1501 gccgccctca cggccatgac gaacgccctc aacgagcagg cgcggtcgag cggcggcaag
     1561 gcggacctgc cgctcctcga gtatcaggac gtcgacgagc cacagctcgc ggacttcaag
     1621 aagttcgccg acgcggtcgc cgcgtgggcg gcgcgcaacc cgcagctcgg accggaccgc
     1681 atcgccgaca cggcgatcgc ggcgatgatc cgcgtctccc ccgactgcca cacctcgtac
     1741 ctcagccgga cgcgacaggt cttccgttcg cggcccgacg tctcgagcgg cgggagtggc
     1801 gcgcgggtgc cggcgaccgg cacgcagctc ttcggtccgg actcgagcgc gctccaggcg
     1861 aagctgctcg ccgacgggat cgcgtacgtc accttcggag agtggacgat gcacgggacg
     1921 tacaacatcg tcgatcagtt gcgcgctgcg ctcgacaagg cgatcgcggc gggtgcgagg
     1981 gcgtggatct tcgacctccg cggcaatccg ggcggcaacg gcgccgagac ggtcgcgagc
     2041 tggttcctga acggcgaggc gatcctgaag accacggtca agacgggcaa cgcgggcacc
     2101 cagtccgcga acaagacgct tcggctgccg ccggcctatc agctgccgat cgtcgtcatc
     2161 gtgaacgggc ggagcgcgtc ggcgagcgag gtgttcgcgc tgtcgctcaa ggagaacggt
     2221 cgcgccacga tcgtgggcgc gacgacgatc ggatgcctcg gcgcggagac gccgacggcg
     2281 atgagtgacg ggtcggagct cgacgtgacc gtgcaggagt acgtgggtgc gcagaccggc
     2341 gccgtctaca acaacaaggg catcccgccc gacgtggccg cggacgacgc gagcgcggtc
     2401 gaccgagcga tcgcgatcct caagacaaag ctttaggtcg ctcgccgcgc gcaacgcgcc
     2461 cgatgtggtg tgcgcggccc catccatgcg acggcacgct tgctcgcgct gcgcatgcga
     2521 cttcggtact aggacccttg cctgcacgta cggtctgcgc gcgttctccg atgacatgtg
     2581 acggttttgg ta
//
LOCUS       DASTZR010000243         3057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_795230, whole genome shotgun sequence.
ACCESSION   DASTZR010000243 DASTZR010000000
VERSION     DASTZR010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795230"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="VFV20_05430"
     CDS             <1..967
                     /locus_tag="VFV20_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU5287825.1"
                     /translation="DNMLASVTAEALGVLPEDVHVVSGDTSLAPVDLGSYSSRVTMMA
                     GNATKNAGLKLRALLFDVASEKLGVPTERLNAAFRRVYDLQDPEKGLSFVEAANLAEA
                     KYGTLVAAGSYRPPAGIGGSYKGAGVGPTPAYSYQAAVAEVTVDLETGQLRVDKITTA
                     HDCGRALNPTNAEGQVEGSAYMGYGEIAVEEQVFRGGLHKKPSLLDYKLPTSLDTPAL
                     EAILVETIDSEGPFGAKEAGEGPLNPVIPAIANAVYDAIGVRFDETPITAEKILDALG
                     KRDNRGVPKDRIGPKGLDGGTGDPKRAMRRLAASTESRERKRSTT"
     gene            967..1971
                     /locus_tag="VFV20_05435"
     CDS             967..1971
                     /locus_tag="VFV20_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEU5287826.1"
                     /translation="MLRLPRFRYVSPPTLEEAVRLRTEHLESSMYVAGGTDVYPKMKR
                     RQFQPELVVSLGRVLPGGISNGTLTSFADQGPRSLGMRIGAGATLTAVSEHPKVREGY
                     TALAEAASVVSTPQLRNMGTIGGNICLDTRCNWYDQSLFWRTAEGFCMKTNAEVVCRV
                     ATSSPRCLAVASADTVPALIALGASVHVTGPSGERVVDLADCYREDGIRYLTLASGEV
                     VTEVRLPPSAGWRSTYLKLRDRNSFDFPIAGLAAAVRYDGTTVAEARVAITALASRPV
                     AVDASALVGTKLDDDAIATVADAVHKAARPMDNTSGTISQRKRASRVFAERALRSLRA
                     "
     gene            1988..2590
                     /locus_tag="VFV20_05440"
     CDS             1988..2590
                     /locus_tag="VFV20_05440"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU5287827.1"
                     /translation="MSEAFRAVAVVTAAGSAERFGGRKLLASVDGEPLLDRTIRALLD
                     GGVSEVIVVVGTDGRAELERDVNAMNDARVRPVENHDPSRGMFSSIQDGVATASGDAL
                     LIMPGDMPFVRPETVRAVIAAYRERPAIVSPRYRGKRGHPVAMPLALREEIRATPSSA
                     TLHEVIKKHADARVDLDVDDPGVIRDVDTPADLERGTGTN"
     gene            2867..>3057
                     /locus_tag="VFV20_05445"
     CDS             2867..>3057
                     /locus_tag="VFV20_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287828.1"
                     /translation="MAPEAQEVSRRSLLKAGVPIVAFIALEAIGGIGATSAAPAPRQV
                     KNASGVIFPDPTLCIGCLT"
BASE COUNT          485 a         1089 c         1067 g          416 t
ORIGIN      
        1 cgacaacatg ctcgcgtccg tcaccgccga ggcgctcggc gtgctcccgg aggacgtgca
       61 cgtggtgtcg ggcgacacga gcctcgcacc ggtggacctc ggctcctact cgagccgtgt
      121 gacgatgatg gccggcaacg cgacgaagaa tgccggcctc aagctgcgcg ccctgctctt
      181 cgacgtcgcg tccgagaagc tcggcgtccc cacggagcgc ctcaacgccg cgttccgccg
      241 cgtctacgac ctgcaggatc ccgaaaaggg cctgtcgttc gtcgaggccg cgaatctcgc
      301 cgaggcgaag tacgggaccc tcgtcgccgc cggctcgtac cggccgcctg ccgggatcgg
      361 cggcagctac aagggcgcgg gcgtcgggcc gacgccggcg tactcgtacc aggccgcggt
      421 ggccgaggtc acggtggacc tcgagaccgg tcagctgcgg gtcgacaaga tcacgacggc
      481 gcacgattgc ggccgtgcgc tgaatccgac caatgccgag ggccaggtcg agggatcggc
      541 ctacatgggc tacggcgaga tcgcggtgga ggagcaggtc ttccgcggcg ggctccacaa
      601 gaagccgagc ctgctcgact acaagctgcc gacgagcctc gacacgcccg cgctcgaggc
      661 catcctcgtc gagaccatcg acagcgaagg cccgttcggc gcgaaggagg cgggcgaggg
      721 tccgctgaac ccggtcatcc cggcgatcgc gaacgcggtg tacgacgcga tcggcgtgcg
      781 cttcgacgag accccgatca ccgccgagaa gatcctcgac gcgctcggca agcgcgacaa
      841 ccgcggcgtg ccgaaggacc gcatcggtcc gaaagggctc gacggcggca cgggcgatcc
      901 gaagcgcgcc atgcgccgcc tcgccgcgtc cacggagtcg cgcgagcgca agcggagcac
      961 gacctgatgc tgcgcctgcc gcgcttccgc tacgtgtcgc cgcccacgct cgaggaggcg
     1021 gtccggctgc gcaccgagca tctggagtcc tcgatgtacg tcgccggcgg caccgacgtg
     1081 tacccgaaga tgaagcggcg acagttccag ccggagctcg tggtgtcgtt gggccgcgtg
     1141 ctgccgggcg gcatcagcaa cgggacgctc acttcgttcg ctgaccaggg gccccgttcc
     1201 ctgggcatgc gcatcggcgc cggcgcgacg ctcaccgcgg tgagcgagca ccccaaagtg
     1261 cgcgagggct acacggcgct cgccgaggcc gcgtcggtcg tttcgacgcc gcagctgcgc
     1321 aacatgggca ccatcggcgg gaacatctgc ctcgacacgc gctgcaactg gtacgaccag
     1381 tcgctcttct ggcgcaccgc cgaggggttc tgcatgaaga cgaacgccga ggtcgtctgc
     1441 cgcgtcgcga cctcgagccc gcgctgcctc gccgtggcga gcgcggacac cgtgccggcg
     1501 ctcatcgcgc tcggcgcctc cgtccacgtc acgggcccga gcggcgagcg cgtcgtcgac
     1561 ctcgccgact gctaccgcga ggacggcatc cgctatctca cgctcgcgtc cggcgaggtc
     1621 gtgacggagg tgcggctgcc accgagcgcc ggctggcgca gcacctatct gaagctgcgc
     1681 gaccgcaact ccttcgactt ccccatcgcc ggcctcgccg ccgcggtccg ctatgacggg
     1741 acgaccgtgg cggaagcgcg cgtcgcgatc acggcactcg cgtcacggcc cgtggccgtc
     1801 gacgcgagcg cgctcgtcgg gacgaagctc gacgacgacg cgatcgcgac cgtcgccgac
     1861 gccgtgcaca aggccgcgcg tccgatggac aacacgtcgg ggacgatcag ccagcgcaag
     1921 cgcgcctcac gcgtcttcgc cgagcgcgcg ctgcgttccc tgcgcgcgtg agcagagcgc
     1981 gaggaacgtg agcgaagctt ttcgcgccgt cgccgtcgtc acagccgcgg gttccgcgga
     2041 gcgcttcggc ggccgcaagc tgctcgcgag cgtcgatggc gagccgctgc tcgaccgcac
     2101 gatccgcgcg ctcctcgacg gcggcgtgag cgaggtgatc gtcgtcgtcg gcacggacgg
     2161 gcgcgccgag ctcgagcgcg acgtcaacgc gatgaacgac gcgcgcgtgc gtccggtgga
     2221 gaaccacgac ccgtcacgcg gcatgttctc ctcgatccag gacggcgtcg cgaccgcgag
     2281 cggggacgcg ctgctgatca tgcccggcga catgcccttc gtacgaccgg agacggtgcg
     2341 cgcggtcatc gcggcatacc gggagcgccc cgccatcgtc tcgccgcggt atcgcggaaa
     2401 gcgcggccat cccgtcgcca tgccactcgc gctccgagag gagatccggg cgacgccgag
     2461 cagcgccacg ttgcacgagg tgatcaagaa gcacgctgat gcgcgcgtgg acctcgacgt
     2521 ggacgatccc ggtgtgatcc gcgacgtgga cacgcccgcg gatctcgagc gcggcaccgg
     2581 cacgaactag gagggggcgg cgacttgcgt cgccaccccc ttcagacctc tggcaggcct
     2641 caaagactgt gtcggtcttg tgtctgtccc ggggtcagcc tagcagaggg gagcccgccg
     2701 gagcctgaga atcgcgagcc gaaatcgggc cattgtccgt gctgtggaac gcggggccga
     2761 ccgcgttgac gagggcaaaa cggcaggcta acttcgcccg aacgccgcac ccaaacgcta
     2821 cgaaggtggg acgacaccgg tgagcgacgg cagaggtcag caggacttgg cacccgaagc
     2881 tcaggaggtc tcccgtcgga gccttctgaa ggccggcgtc cccatcgtcg cgttcatcgc
     2941 cctggaggcc atcggtggga tcggcgcgac gtccgcggcg ccggcgccgc gtcaggtcaa
     3001 gaacgcctcg ggcgtcatct tccccgatcc gaccctctgc atcggttgcc tcacctg
//
LOCUS       DASTZR010000244         4042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_901431, whole genome shotgun sequence.
ACCESSION   DASTZR010000244 DASTZR010000000
VERSION     DASTZR010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901431"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            333..773
                     /locus_tag="VFV20_05450"
     CDS             333..773
                     /locus_tag="VFV20_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5287829.1"
                     /translation="MTALGERSESILEMALTADRPNVTTELVRRYLDDPVTSPKMRLA
                     AVDALARVGTTHAREILSRLSQRRDEVGKAATSAQHRPGVGLSEREIEVLSLAADGLT
                     NKQIADKLFLSPHTIARHLANARGKLGASNRAEAAVRLHRAGID"
     gene            complement(1031..1360)
                     /locus_tag="VFV20_05455"
     CDS             complement(1031..1360)
                     /locus_tag="VFV20_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEU5287830.1"
                     /translation="MKLVIAIIHREDAGALVDALTDKEYRVTRLQSQGGFLKQSNASI
                     LVGVEEAQVEDVLTTIRETCHARTQHISPMPPIMEPGEFYMPYPVEVTFGGATVFVLD
                     VARYERI"
     gene            complement(1380..2018)
                     /gene="tmk"
                     /locus_tag="VFV20_05460"
     CDS             complement(1380..2018)
                     /gene="tmk"
                     /locus_tag="VFV20_05460"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013557528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU5287831.1"
                     /translation="MKRGLFLSFEGGEGSGKSLQTRNLARYLEERGREVVLTREPGGT
                     PAGERIRDILLHAKEAPLAPEAQVLLYSAARAQLVRDVIRPALEAGSIVIADRYYDST
                     LAYQGHGHGVSLDLIRDVTALAVAGLVPDRTFLLDVPVQVGLDRSGWRAAAKWDRFEV
                     LEADFHERVREGYRRLAAAEPGRWVLVNGDRDEHAISTDIRREVDSLLGARA"
     gene            complement(2015..3538)
                     /locus_tag="VFV20_05465"
     CDS             complement(2015..3538)
                     /locus_tag="VFV20_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HEU5287832.1"
                     /translation="MQQQSDELEALLRGLPADLGARVRALDGLDGLLEIVMDLGRLPE
                     ARFAGREETLSRREVSAEDIAYVISHIGQFGGDNRAGIERTLHRISALRNRAGKVVGL
                     TLRVGRAVYGTVEILRDIVESGKSTLFLGRPGVGKTTMLREVARVLADDLAKRVVVVD
                     TSNEIAGDGDIPHAGIGRARRMQVPTPSLQHAVMIEAVENHMPEVVVIDEIGTEQEAA
                     AARTIAERGVQLIATAHGNTLENLMLNPTLTDLVGGIQTVTLSDEEARRRGTQKSVLE
                     RKAPPTFQVLVEIQAYQRVAIYHDVAQTVDAVLLGMPVSPEIRARGVDGEIERTTQEP
                     TRAPAEAVERRSTPRIPIGAEGDRQTIKVYPFGVSWNRVEDAARGLGMPIAVVREPED
                     ADVVITLKNYYRRKTPRLRNAESSGIPIYIARSNSSSQIAAALASVAELRRGPEDRAL
                     EEAQEAIDAVASGKTDEVELAPQNAYVRRLQHELAERASLSSRSTGHEPVRRVQIYR"
     gene            3616..>4042
                     /locus_tag="VFV20_05470"
     CDS             3616..>4042
                     /locus_tag="VFV20_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287833.1"
                     /translation="MSQGERGRSVKLDASDHEGIAQLGAALRTAGYTPDRMKVALRAE
                     GDNLTPRSEDVAVVLRLLPERDRLADVIRLFLLGVALDETRVADALAPLTVDHGLRLG
                     ILERSGADVRAAVRILPSGDLLFACDRELESSPDLPPDHV"
BASE COUNT          589 a         1433 c         1390 g          630 t
ORIGIN      
        1 gtgtggcggc cgaaatcgaa gacggaacac cgagcaacaa gaccgagctt gcagggctat
       61 tggcccgtgc tcgacgagct gcaccgggtc taaaagcgct tccagccctc ctgcagggcc
      121 gcgccgagaa gcggtacgag gcattcgctg atgcggagcg actcgctcga cgatcaggga
      181 atacgtggct tcagatcagt gcgctgacct ggatggcgtc gctagacctt agtccggtca
      241 tagcgcaccg actccgtgtg ctgctcgacg tcacagggtg gcggcgtcca gtcctcgtgc
      301 ctacggaagt ggcggctgac gcggctctcg gcatgacggc gctgggtgag cggtcggagt
      361 cgatcctcga gatggcgctc acggcggacc gtccgaacgt caccacggag ctcgtgcgcc
      421 ggtacctcga cgaccctgta acgtcgccga agatgcggct tgccgcggtc gatgctctcg
      481 cgcgcgtcgg caccacgcac gcgcgcgaga tcctgtcgcg gctctcgcag cgacgcgacg
      541 aggtcgggaa ggcggcgacg agcgcgcaac atcgacccgg cgtcggcctc agcgaacgcg
      601 agatcgaggt gctcagcctc gcggccgacg gcctcacgaa caagcagatc gccgacaaac
      661 tgttcctcag ccctcatacg atcgcgcggc atctcgcgaa cgcacgagga aagctcggtg
      721 cgtcgaaccg ggcggaggcc gcggttcgac tacaccgagc cgggatcgat taggcggcgc
      781 gcggcccctc gtcgtgcggc ttgcggccgc ccgcgatcaa ccgaagccac tcccccgtcg
      841 acgtcagacg tacgcgaagg tgacgctgtt tcgacgggaa cggaatgatc ttcgccgtac
      901 gggtcgtcat cgccatctcc ccctgtgacc ggggaaggtc ccggtcacgc ggcgacgatg
      961 ccgccagcgc cgggcctcgc catcgcgcag attgaccacc cgcgcctagc caaatcggga
     1021 tgacgcctgg ctagatgcgc tcgtacctcg cgacgtcgag cacgaagacg gtcgcgccgc
     1081 cgaacgtcac ctcgaccgga tagggcatgt agaactcgcc cggctccatg atcggcggca
     1141 tcgggctgat gtgctgcgtt cgcgcatggc aggtctcgcg gatcgtcgtg agcacgtcct
     1201 ccacctgcgc ctcctcgacg ccgacgagga tcgaggcgtt gctctgcttc aggaatccac
     1261 cctgcgattg cagccgggtg acgcggtact ccttgtcggt gagcgcgtcg acgagggcgc
     1321 cggcgtcctc acggtggatg atcgcgatca cgagcttcac ggccgcgact ctagcgcgtc
     1381 tacgcgcgcg cgccgagcag actgtcgacc tcgcgacgta tatccgtgct gatcgcgtgc
     1441 tcgtctcgat caccattgac gagcacccat cgcccgggct ccgcggccgc cagccggcgg
     1501 tagccctcgc gcacgcgctc gtggaaatcg gcctcgagca cctcgaaccg atcccacttg
     1561 gccgccgcgc gccagccgga gcgatcgagg cccacctgga ccggcacgtc gaggaggaac
     1621 gtgcggtcgg gcacgagacc ggcgaccgcg agcgccgtga cgtcgcgtat gagatcgagc
     1681 gagacgccgt gcccgtgacc ctggtacgcg agcgtcgagt cgtagtagcg gtcggcgatc
     1741 acgatgctgc cggcctcgag cgcggggcgg atgacgtcgc gcaccagctg cgctcgtgcc
     1801 gcgctgtaga gaaggacctg cgcctcgggc gccagcggcg cctccttcgc gtgcagcagg
     1861 atgtcgcgga tgcgctcgcc cgcgggcgtg ccgccgggct cacgcgtgag caccacctcg
     1921 cggccgcgct cctcgaggta gcgagcgagg ttccgcgtct ggagcgactt gccggagcct
     1981 tcaccgccct cgaacgacag gaagagaccg cgtttcaccg atatatctgg acgcgtcgta
     2041 ccggctcgtg cccggtcgat cgtgacgaga ggctggcgcg ctccgcgagc tcgtgctgca
     2101 ggcggcgtac gtacgcgttc tgcggagcga gctcgacctc gtcggtcttg cccgacgcga
     2161 ccgcgtcgat cgcctcctgc gcctcctcga gggcgcggtc ctctggcccg cggcgcagct
     2221 ccgcgaccga cgcgagcgcc gcggcgatct gactcgacga gttgctgcgc gcgatgtaga
     2281 tcgggatgcc ggacgactcc gcgttgcgca ggcgcggtgt cttgcggcgg tagtagttct
     2341 tcagcgtgat gacgacgtcc gcgtcttccg gctcgcgtac gacggcgatc ggcattccga
     2401 gcccgcgcgc ggcgtcctcc acgcggttcc acgacacgcc gaacgggtag accttgatcg
     2461 tctgacggtc gccctcggcg ccgatgggga tccgcggcgt gcttcgccgc tccaccgcct
     2521 ccgccggcgc ccgggtcggc tcctgcgtgg tgcgctcgat ctcgccgtcg acgccgcgcg
     2581 cgcggatctc gggcgacacc ggcatgccga gcagcaccgc gtcgacggtc tgcgccacgt
     2641 cgtgatagat cgcgacgcgc tgatacgcct ggatctcgac gagcacctgg aacgtcggcg
     2701 gcgccttgcg ctcgagcacc gacttctgcg tgccgcggcg gcgtgcctcc tcatcggaga
     2761 gcgtgacggt ctggatgccg ccgacgagat cggtgagcgt gggattcagc atgaggttct
     2821 cgagcgtgtt cccgtgcgcc gtcgcgatga gctgcacgcc acgctcggcg atcgtgcgcg
     2881 ccgcggcggc ctcctgctcg gtgccgatct cgtcgatgac gacgacctcg ggcatatggt
     2941 tctcgaccgc ctcgatcatc acggcgtgct gcagcgacgg cgtcggcacc tgcatgcgcc
     3001 gcgcgcggcc gatgccggcg tgcgggatgt cgccgtcgcc ggcgatctcg ttcgacgtat
     3061 ccacgaccac cacgcgcttc gcgaggtcgt ccgcgaggac ccgcgccacc tcgcgcagca
     3121 tcgtcgtctt gcccacgccc gggcggccga ggaagagcgt gctctttccg gactcgacga
     3181 tgtcgcgaag gatctcgacg gtcccgtaca cggcgcggcc gacgcgcagc gtgagcccca
     3241 cgaccttgcc cgcgcggttg cgcagcgcgc tgatgcgatg gagcgtgcgc tcgatgcccg
     3301 cgcggttgtc gccgccgaac tgtccgatgt gcgagatcac gtacgcgatg tcctcggccg
     3361 agacctcccg gcgcgagagc gtctcctcgc gtccggcgaa gcgcgcctcg ggcaggcggc
     3421 cgaggtccat cacgatctcg agcagtccgt cgaggccgtc gagcgcgcgg acgcgcgcgc
     3481 cgaggtcagc ggggagtccg cggaggagag cctcgagctc gtcgctctgc tgttgcatcg
     3541 ctatctcacc gaccggctcg cggtattcca ccgcattccg gtagagcata tcgaggaccg
     3601 ccgtagcatc cggcggtgag ccaaggggag agggggcggt cggtcaagct cgacgcatcc
     3661 gaccacgagg ggatcgcgca gctcggcgcc gcgctgcgca ccgccggcta cacgccggat
     3721 cgcatgaagg tcgcgctccg cgcggagggc gacaacctca cgccgcggtc cgaggacgtc
     3781 gccgtcgtcc tgcggctcct tcccgagcgt gaccgtctcg ccgacgtcat ccggctcttc
     3841 ctgctcggcg tcgcgctcga cgagacgcgc gtggccgacg cgctcgcgcc gctgaccgtg
     3901 gaccacgggc tccgcctcgg catcctcgag cgctccggcg cggacgtgcg cgccgcggtg
     3961 cgcatcctcc cgtcgggcga cctcctcttc gcgtgcgacc gcgagctcga gtcgagcccg
     4021 gaccttccgc cggaccacgt ca
//
LOCUS       DASTZR010000245         7190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_62653, whole genome shotgun sequence.
ACCESSION   DASTZR010000245 DASTZR010000000
VERSION     DASTZR010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7190
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62653"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFV20_05475"
     CDS             complement(<1..183)
                     /locus_tag="VFV20_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008735588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HEU5287834.1"
                     /translation="MPARLLVKEENTVAEHPATELSELRGGHACWLDIADPDSDAMRL
                     AAEELALHPLAVEDAQH"
     gene            complement(204..569)
                     /locus_tag="VFV20_05480"
     CDS             complement(204..569)
                     /locus_tag="VFV20_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5287835.1"
                     /translation="MRYHEARPVLVVDDDDAIRAAEHEVLEDHGFRVIEARDGAEAMR
                     AIQQDPPSLVVLDVQMPGVDGPAFARALRVALRRVPLVILTAAGDPRREADRCNAEAY
                     LAKPFDAHELVRVVRRFSS"
     gene            660..1562
                     /locus_tag="VFV20_05485"
     CDS             660..1562
                     /locus_tag="VFV20_05485"
                     /inference="COORDINATES: protein motif:HMM:NF024795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HEU5287836.1"
                     /translation="MGRLLALAAGLLAASCGVGVPAASPTPAAPLPVAELKYRVMEAG
                     GRIEFCDPDFYPVARADETQRARERIGEIQKDPDTYAAIVRRVGTDELAVYREWKALS
                     ALALGPTSFGGPSVAQSHSFAYRAAGTLGAATPAPKRATGVEIEGNVDPFGAVKITKR
                     TDVGPLNCPICLARGTRIATPSGEIAVEDLRVGDAVWTQDAGGVRVAAPIVAIGSAPV
                     AAAHEVVRLVLADGRTLRASLGHPAADGRNLGELRVGDVLDGTRIVSVEREPYAGRAT
                     FDILPAGATGIYWAEGVALGSTLR"
     gene            complement(1591..3441)
                     /locus_tag="VFV20_05490"
     CDS             complement(1591..3441)
                     /locus_tag="VFV20_05490"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU5287837.1"
                     /translation="MTLGSFSMPRPLSDFDPRERVAIVPHTTRRFACRASIAIAPDGH
                     AVAVVTDRGAGTYEAWTVSASGGTPRRAVTIEDHAVRSLCWSASGELLAAADKGGTEL
                     HQIYIRHPDGPQLPVSVDPAGKVQRLLSWNATSPDGTHVAFSSNARASADMDVVLGDL
                     ETRTERPLLTDAAWHVVGGWSPDGAQLLVMRVHDNTTQDLLVVDPHTGAAREITRHSE
                     DTSHVPAGWLADGRVVEITDIDDEHLWLAALDPKTGMREAIDRGAWDVELAASSGDGR
                     VQAWSVNEDGYSKLRWRVDGGPVRERDLRGVCENVVVSHDGSRIAYLRIAATEAWQVW
                     TLDTHSGEARVALASSYAVPSADLVEPELIRIAGPEGGIPCFIYRPKGARAPYPAVLY
                     PHGGPEGQSRPAFTPHLTHLAALVDRGIALVVPNIHGSTGYGRAWQAAIHKDWGGIDL
                     RDLRAVTAWMQQQPDFDQSRLAVYGGSYGGFATLLCVTQLPEVWRCAVDVFGVANLVT
                     MLENAQPNWRRFLARWIGELPGDREKLVERSPITHIENVRCPMLILQGSNDPRVPQEE
                     SDQVVAKLRAMGRRVDYVVYPDEGHGFTKKKNADDAYGRIVEFLTKELLA"
     gene            complement(3438..4367)
                     /locus_tag="VFV20_05495"
     CDS             complement(3438..4367)
                     /locus_tag="VFV20_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEU5287838.1"
                     /translation="MATRKKLEKDSKVLIGTCSWTDPTLLESGWYPPEVAKKPDERLR
                     YYAERFALVENDASYYAIPDPKQAKLWAERTPEEFTMNFKAFGAITTHPIDPKRLPKE
                     LRESLPKDVAAKRRVYPKDLPKDVYDEIHARFWVGLEPLRKAGKLGAILLQYPDWFPI
                     GSKNKSEILRARELLPDDRLAVEFRNRTWMEERNQKETLGFLRDNGLAYVSVDEPQGF
                     PSSIPPVAEATAPLAIVRFHGRNAENWKKPGLTAAERFDYSYRLPELKEWLPKIKRLE
                     EEAEEVHLLMNNCYGDKAVRNAADLAGMLAKKS"
     gene            complement(4593..5687)
                     /locus_tag="VFV20_05500"
     CDS             complement(4593..5687)
                     /locus_tag="VFV20_05500"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEU5287839.1"
                     /translation="MTTVLYVGSAVLAFGLAAAITPLVARLAERLGMLDVPGGRRLHP
                     RPIARPGGLAIAIAFGLTILAFWLVDRYAGRPFLIPEEVRSPRFTLTALAAILGMTVG
                     FVDDLFELRARWQFLGQIIVAAIIIAAGIRIEFIDDPFGGGLIQLGMPIAVAFTFFWI
                     VGMNNALNFIDGLDGLAAGVSAIAAATLGAIALLPQVSEPFVAWMAFSLAGAAIGFLL
                     FNFHPARIFLGSTGVVFVGTMLAVLSIFGTAKVAAALLVLGVPIIDTFYVLLRRTLQG
                     RPPFAPDRGHFHHRLLDVGLSHSQAVLLIYALTLGFGTLAFLTSGRTQVLTFVGAVLL
                     FGVAVVVLAQRAPKAEELDPSLYAEEADRF"
     gene            5827..6498
                     /locus_tag="VFV20_05505"
     CDS             5827..6498
                     /locus_tag="VFV20_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU5287840.1"
                     /translation="MKQASGSAAVVRRAKRTHAKRGTIIPLPTTARGQRTRQKLLDSA
                     ESVFGERGFHNAGIIEITQRADVALGTFYLYFPDKKSIFDDLVHTLNTRLRKTISDRV
                     RDVDDRLEQEVLGFETFFEFVRRHRNLYRVILQAETVDEDLYRWHYRTLADGYVRGIR
                     KAQTAGQVRSDLDAETMAYALMGMAEALGSRYVLWDGTLPPRSTKRTIRAFLEGGMRR
                     PKLKR"
     gene            6495..>7190
                     /locus_tag="VFV20_05510"
     CDS             6495..>7190
                     /locus_tag="VFV20_05510"
                     /inference="COORDINATES: protein motif:HMM:NF020134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HEU5287841.1"
                     /translation="MTTVGIVDVCSYSPTAVQTADQIAALSRIPANVIREKFGLDGKH
                     IASADEHASVLAAKAAAPILERHGGRVDALLYMGSPHRDFPVWSAAPKVQQLLGERAQ
                     GSLAFDIQGVSAGAPMAMQVAKGLLCANEWMQQIVLAGGSREHDLIDYENERSRFMFN
                     FGAGGAALLLRRGHPENLVLESAGFTDGSFADDVMVPAGGSREPASERTVRERRHALD
                     VRDPAKMKRRLDPV"
BASE COUNT         1114 a         2484 c         2565 g         1027 t
ORIGIN      
        1 atgctgggcg tcctcgacgg cgagcggatg gagcgccagc tcctccgccg cgagccgcat
       61 cgcgtcgctg tccgggtccg cgatgtcgag ccaacaggcg tgcccgccgc gcagctcgga
      121 gagctccgtc gcggggtgtt cggcgacggt gttctcttcc ttgacgagca ggcgtgcagg
      181 catcgggcgg aggatattcg gcgtcaggac gaaaagcgac gcaccacccg gacgagctcg
      241 tgcgcgtcga agggcttggc gagatacgcc tcggcgttgc agcgatccgc ctcacgtcgc
      301 ggatcgcccg ccgcggtgag gatgacgagc ggcacgcggc gcagcgccac acgcagcgcg
      361 cgggcgaagg cagggccgtc cacgcccggc atctggacgt cgagcacgac gagggatggc
      421 ggatcctgct ggatcgcgcg catcgcctcg gcgccatccc gcgcctcgat cacgcggaag
      481 ccgtggtcct cgaggacctc gtgctcggcc gcacgtatcg cgtcgtcatc gtcgacgacg
      541 aggaccggcc gggcttcgtg gtatcgcata cctcgggtac atagcacttg acgtaccggg
      601 ttatgggacg cctgctcgtc tgccggaacc gggggctccg ggccggcgtt ctcacgacag
      661 tgggaaggct tctggcgctc gccgcggggc tcctcgccgc gtcgtgcggc gtgggcgtcc
      721 cggccgccag cccaacgccg gcggcgccgc tgcccgtggc ggagctcaag taccgggtca
      781 tggaggcggg tggccggatc gagttctgcg atccggactt ctatcccgtc gcgcgcgccg
      841 acgagaccca gcgcgcgcgg gagcgcatcg gcgagatcca aaaggacccc gacacgtacg
      901 cggccatcgt gaggcgcgtc gggaccgacg agctcgcggt gtaccgcgag tggaaggccc
      961 tctccgcgct cgccctcggg ccgacgtctt tcggcggacc gagcgtcgcg cagtcgcact
     1021 ccttcgcgta ccgcgcggcg ggcacgctcg gagcggccac acccgcgccc aagcgagcca
     1081 ccggcgtcga gatcgagggg aacgtggatc cgttcggcgc cgtcaagatc acgaagcgca
     1141 ccgacgtcgg cccgctcaac tgcccgatct gcctcgcgcg cgggactcgt atcgccacgc
     1201 catcgggcga gatcgccgtc gaggacctcc gcgtcggcga cgccgtgtgg acccaggacg
     1261 cgggcggcgt gcgcgtcgcc gcgccgatcg tcgccatcgg cagcgcaccg gtcgcggcgg
     1321 cgcacgaggt cgtgcgcctc gtgctcgcgg acggccgaac gctccgggca tcgctcgggc
     1381 atcccgccgc ggacggtcgc aacctgggcg agctgcgcgt cggcgacgtg ctggatggga
     1441 cgcggatcgt gtcggtggag cgcgagccgt acgcgggtcg cgcgacgttc gacatcctgc
     1501 ccgcgggcgc gaccgggatc tactgggcgg agggcgtcgc gctgggcagc acgctgcgct
     1561 gaccgcgcgg catcgaggcg cgctgccgcg tcacgcgaga agctccttcg tgaggaactc
     1621 gacgatccgc ccgtaggcat cgtcggcgtt cttcttcttc gtgaagccgt gcccctcgtc
     1681 gggatagacg acgtagtcca cgcgccggcc catggcccgc agcttcgcga cgacctggtc
     1741 cgattcctcc tgggggacgc gcgggtcgtt ggagccctgc aggatgagca tcgggcagcg
     1801 cacgttctcg atgtgcgtga tcggcgagcg ctccaccagc ttctcgcgat cgccgggcag
     1861 ctcgccgatc cagcgcgcga ggaaccggcg ccagttcggc tgcgcgttct cgagcatggt
     1921 cacgaggttc gcgacgccga agacgtcgac ggcgcagcgc cacacctcgg gcagctgcgt
     1981 gacgcacagg agcgtggcga aacctccgta cgagccgcca tacacggcga gacggctctg
     2041 gtcgaagtcc ggctgctgct gcatccacgc ggtgacggcg cgcaggtcgc ggaggtcgat
     2101 gccaccccag tccttgtgga tcgccgcctg ccacgccctg ccgtagccgg tcgagccgtg
     2161 gatgttgggc accacgagcg cgatgccgcg gtcgacgagc gcggcgaggt gcgtgagatg
     2221 cggcgtgaag gccggccgcg actggccctc gggtccgccg tgcggataca ggaccgccgg
     2281 atacggcgca cgcgcaccct tcggccgata gatgaagcac gggatcccgc cctccgggcc
     2341 cgcgatgcgg atgagctccg gctcgacgag gtccgcgctc ggcaccgcgt aggagctggc
     2401 gagcgccacg cgcgcctcgc cgctgtgcgt atcgagcgtc cacacctgcc acgcctcggt
     2461 cgcggcgatg cggaggtacg cgatgcgcga gccgtcgtgg ctcacgacga cgttctcgca
     2521 cacgcctcgc aggtcacgct cgcggaccgg gccgccgtcc actcgccagc gcagcttcga
     2581 gtagccatcc tcgttcaccg accacgcctg gacgcgccca tctcccgacg atgcggcgag
     2641 ctcgacgtcc caggcgccgc ggtcgatggc ctcccgcatc ccggtcttcg gatcgagcgc
     2701 cgccagccag agatgctcgt cgtcgatgtc ggtgatctcc acgacgcgcc cgtcggcaag
     2761 ccacccggcc ggcacgtgcg aggtgtcctc gctgtggcgc gtgatctcgc gcgccgcacc
     2821 cgtgtgcggg tcgacgacga gcaggtcctg cgtcgtgttg tcgtgcacgc gcatgacgag
     2881 cagctgcgcg ccgtcgggcg accacccgcc gacgacgtgc cacgccgcat cggtgagcag
     2941 tggccgttcc gttctcgtct cgaggtcgcc gaggacgacg tccatgtccg ccgacgcgcg
     3001 cgcgttgctc gagaacgcga cgtgcgtgcc gtcgggggag gtcgcgttcc acgagagcag
     3061 acgctgcacc tttccggcgg gatcgaccga gaccggcagc tgcggaccgt cgggatggcg
     3121 gatgtagatc tgatgcagct cggtgccgcc cttgtccgcc gccgcgagca gctcgccgga
     3181 cgcgctccag cagaggctcc gcaccgcgtg gtcctcgatg gtcaccgcgc gccgcggcgt
     3241 gccgccgctc gcgctcaccg tccaggcctc gtacgtgccc gcgcctcggt cggtcaccac
     3301 ggcgacggcg tgaccgtccg gcgcgatcgc gatgctcgcc cggcacgcga accgccgcgt
     3361 cgtgtgcggc acgatggcga cgcgctcgcg ggggtcgaag tcggataggg gccgcggcat
     3421 ggagaacgag cctagcgtca tgacttcttc gccagcatgc ccgccaggtc ggcggcatta
     3481 cggacggcct tgtccccgta gcagttgttc atgaggagat gcacctcttc ggcctcctcc
     3541 tcgagccgct tgatcttcgg cagccactcc ttgagctcgg gcagcctgta ggagtagtcg
     3601 aaccgctccg cggcggtgag cccaggcttc ttccagttct cggcgttgcg cccgtggaag
     3661 cgcacgatcg cgagcggcgc ggtggcctcg gcgacgggcg ggatcgacga cgggaagccc
     3721 tggggctcgt cgacggacac gtacgcgaga ccgttgtcgc gcaggaagcc gagggtctcc
     3781 ttttgattgc gctcctccat ccaggtccgg ttgcggaact cgacggcaag tctgtcgtcg
     3841 ggcagcagct cgcgcgcgcg gaggatctcg ctcttgttct tggagccgat cgggaaccag
     3901 tcgggatact gcagcaggat cgcccccagc ttgccggcct tgcgcagcgg ctcgagcccg
     3961 acccagaagc gggcgtggat ctcgtcgtac acgtccttcg gaaggtcctt cggatacacg
     4021 cgccgcttcg cggcgacgtc tttcggaagc gactcgcgca gctccttcgg caggcgcttc
     4081 ggatcgatcg ggtgcgtcgt gatcgcgccg aacgccttga agttcatcgt gaactcctcc
     4141 ggcgtgcgct cggcccagag cttcgcctgc ttggggtcgg ggatcgcgta gtacgaggcg
     4201 tcgttctcca cgagcgcgaa tcgctccgcg tagtagcgga gccgctcgtc cggcttcttc
     4261 gcgacctccg gcggatacca accggactcg agcagcgtgg gatccgtcca cgagcacgta
     4321 ccgatgagga ccttcgaatc cttctcgagc ttcttgcgcg tcgccacgca gagagtgtgc
     4381 gccgcgcgca tgggcagcag aacgaagagg acgcgcgggc gtgccaggcg acgcgcggag
     4441 cggcgctcct cgacgagcgt ctgcgcccag ccggcccctg cgcggcgcgc gcggtgaacg
     4501 gcgggccggc gttgccagca gcgaggagag gagtgccacc ccgcgcgtcg ccgccgagac
     4561 gatccgcatg acgcgagcgc gaggcgagcg cgctagaagc ggtccgcctc ctcggcgtac
     4621 aggctcggat ccagctcctc ggcctttggc gcgcgctggg cgagcacaac gacggcgacg
     4681 ccgaagagga ggacggctcc gacgaacgtc agcacctgcg tgcgtccgct cgtgaggaag
     4741 gcgagcgtgc cgaagcccag cgtgagcgcg tagatcagca ggacggcctg cgagtgcgac
     4801 aggccgacgt cgagcagccg gtggtggaag tggccgcgat ccggagcgaa cggcggccgt
     4861 ccctgcagcg tgcgccgcag cagcacatag aaggtgtcga tgatcggcac gccgaggacg
     4921 agcagggcgg ccgcgacctt ggcggtgccg aagatcgaca acaccgcgag catcgtcccg
     4981 acgaagacga ccccggtcga gcccaggaag atgcgcgccg ggtggaagtt gaacaggagg
     5041 aagccgatcg ccgcgccggc gagcgagaac gccatccacg cgacgaacgg ctcggagacc
     5101 tgcggcagca gtgcgatggc tccgagggtc gcggcggcga tcgcggacac gccggccgcg
     5161 aggccgtcga ggccgtcgat gaagttgagc gcgttgttca tgccgacgat ccagaagaac
     5221 gtgaacgcga cggcgatcgg catgccgagc tgtatgagcc cgccgccgaa aggatcgtcg
     5281 atgaactcga tgcggatgcc cgcggcgatg atgatcgcgg cgacgatgat ctggcccagg
     5341 aactgccacc gcgcgcgcag ctcgaagagg tcgtcgacga agccgaccgt catccccagg
     5401 atcgcggcga gcgccgtcag cgtgaagcgc ggcgagcgca cctcttcagg gatgaggaac
     5461 ggccgtcccg cgtagcggtc gacgagccag aacgcgagga tcgtgaggcc gaacgcgatg
     5521 gcgatcgcga gcccgcccgg gcgcgcgatg ggccgtggat gtagtcgcct gccgccgggg
     5581 acgtcgagca tcccgaggcg ttcggcgagg cgggcgacga gcggcgtgat cgcggccgcg
     5641 agtccgaagg cgagcacggc cgatccgacg tagagaacgg tcgtcacagg tataggcacg
     5701 atagcgcgct tgccacgcgc ggtcggtccc gcggccgccg aggaccgccc gacccgcatc
     5761 ggcgagcagc ccacaggcaa gcgttgcgcc gcgaaggcgc ggggcgtatc gtcccaaacc
     5821 tgatacatga aacaggcttc aggttcggct gcggtcgtcc ggcgagcgaa gaggacgcac
     5881 gcgaagcgcg gaacgatcat cccgctcccc acgaccgccc gcggtcagcg cacgcgacag
     5941 aagctgctcg attccgccga gtcggtcttc ggggagcgcg gcttccacaa cgccggcatc
     6001 atcgagatca cgcagcgcgc cgatgtcgcg ctcgggacct tctacctgta cttcccggac
     6061 aagaagtcga tcttcgacga cctcgtccac acgctgaaca ctcgactgcg caagaccatc
     6121 agcgaccgcg tccgcgacgt cgatgaccgg ctcgagcagg aggtcctcgg cttcgagaca
     6181 ttcttcgagt tcgtacggag gcaccgcaac ctgtaccgcg tgatcctcca ggccgagacg
     6241 gtcgacgagg atctgtaccg ctggcattac cgcacgctcg cggacggcta cgtccgcggt
     6301 atccggaagg cgcagaccgc cggacaggtc cgatcggacc tcgatgccga gaccatggcg
     6361 tacgcgctca tggggatggc agaggcactc ggctcgcgct acgtgctgtg ggacggcacg
     6421 ctgccgccgc ggtcgacgaa gcgcaccatc cgcgcgttcc tcgagggcgg catgcgacgg
     6481 ccgaagctca agcgatgacg acggtcggga tcgtcgacgt ctgctcgtac tcacccaccg
     6541 cggtccagac cgcggaccag atcgcggcgc tctcgcgcat accggcgaac gtcatccgcg
     6601 agaagttcgg tctcgacggc aagcacatcg caagcgccga cgaacacgcc tcggtcctcg
     6661 cggcgaaagc cgcggcaccc atcctcgaga ggcacggtgg ccgcgtcgac gcgctgctgt
     6721 acatgggcag ccctcaccgc gactttcccg tctggagcgc cgcgccgaag gtgcagcagc
     6781 tgctcggcga acgggcgcag ggctcgctcg cgttcgacat ccagggcgtc tccgccgggg
     6841 cgcccatggc gatgcaggtg gcgaaggggc tgctgtgcgc gaacgagtgg atgcagcaga
     6901 tcgtcctggc gggcggatcc cgcgagcacg acctcatcga ctacgagaac gagcgctcgc
     6961 gcttcatgtt caacttcggc gcggggggcg cggcgctgtt gctgcggcgc ggccaccccg
     7021 agaacctcgt gctcgagagc gccggcttca ccgatggatc gttcgcggac gacgtcatgg
     7081 tcccggcggg cggttcgcgt gagccggcca gcgagcgcac cgtccgcgag cggcggcacg
     7141 cgctggacgt gcgcgacccc gccaagatga agcgccgcct cgacccagtg
//
LOCUS       DASTZR010000246         2694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_431588, whole genome shotgun sequence.
ACCESSION   DASTZR010000246 DASTZR010000000
VERSION     DASTZR010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431588"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..1367
                     /locus_tag="VFV20_05515"
     CDS             90..1367
                     /locus_tag="VFV20_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU5287842.1"
                     /translation="MTAESERMVVREVLDNGLRLITESMPHVRSVTIGVWLMRGSRHE
                     SEERGGIAHFVEHMLFKGTDTRSAEDIAQAIDSIGGQLDAFTAKEYASYYIKVLDEHL
                     PLAVDLLADIVLRPAFPIDEIEREKKVILEEIKMVEDTPDDLVHELFTQHFWEGHPLG
                     RPILGSKETVEQFALDSLRQYFRDAYVARNMIISAAGNLEHAVVRDLVERAFGELASD
                     GEPIDGIAPRVFPQVITRTKELEQSHLCLGTNGYPQNHEDRYVTYILNTVLGGSMSSR
                     LFQNIREKRGLAYSVFSGLSAYRDAGNITIYAGCATDAVDEVVDLCVEELRGMKRTPV
                     AEPELRRAKDHLKGSLMLSLENTASRMSHLARQEIYFDRHFGLDETLAGVERVTADDV
                     QRVAHDLFANGSLAATVLGPSVPTLTRERLDLT"
     gene            1364..2674
                     /gene="purB"
                     /locus_tag="VFV20_05520"
     CDS             1364..2674
                     /gene="purB"
                     /locus_tag="VFV20_05520"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU5287843.1"
                     /translation="MIRRYTNPEMGAIWSDQRRYETWLEVELAAADAMAAAGIVPAEA
                     ARELRAKASFDLERIEEIEQTTQHDVIAFTTAVAERVGPSARWLHFGLTSSDVVDTAQ
                     ALQMRQACDLIVKGLAGLMDAVRTRAEEHRRTPMIGRTHGVHAEPMTFGLKLALWYAE
                     LQRDVDRVLRARDTIAVGKISGAVGTFAHLDPSIEAAVCERLGLQAAPVSSQVIQRDR
                     HAELVAALAITAASLEKFALEIRGLQKTEIGEVEEPFGKGQKGSSAMPHKRNPIGCEQ
                     ITGLARLIRGNAVAALENVALWHERDISHSSVERVILPDSFIALDHMLRRFTRIVRGM
                     VVNPQRMRENLERSRGVVFSGTVLLELARRGISREQAYEWVQRNAMRAFHEQRDFKSL
                     LLADPDVMRVLTADEIEKAFDLDDQFRNVDAIFERVFRTVPAAV"
BASE COUNT          465 a          904 c          919 g          406 t
ORIGIN      
        1 gaacttcttg taatcccttt tcttgcaatc acttgcggct gtctgccgag aacgccggcg
       61 ggccccgccc ggcacggagg ccccgagcag tgacagcaga atctgagcgc atggtcgtcc
      121 gcgaggtgct cgacaacggc ctacgactca tcaccgagtc catgccgcac gtgcggtcgg
      181 tgaccatcgg cgtgtggctc atgcgcgggt cgcgtcacga gagcgaggag cgcggcggca
      241 tcgcgcactt cgtcgagcac atgctcttca agggcaccga cacgcgcagc gccgaggaca
      301 tcgcgcaggc gatcgactcg atcggcgggc agctcgacgc gttcacggca aaggaatacg
      361 ccagctacta catcaaggtg ctcgacgagc acctgccgct tgccgtcgat ctgctggcgg
      421 atatcgtgct ccgcccggcg tttccgatcg acgagatcga gcgcgagaag aaggtcatcc
      481 tcgaagagat caagatggtc gaggacacgc ccgacgacct cgtccacgag ctcttcacgc
      541 agcatttctg ggagggccac ccgctcggcc gcccgatcct cggatcgaag gagacggtcg
      601 agcagttcgc gctcgactcg ctgcgccagt acttccgcga cgcctacgtc gcgcgcaaca
      661 tgatcatctc ggcggccggc aacctcgagc acgccgtcgt ccgcgatctc gtggagcgcg
      721 cgttcgggga gctcgcgagc gacggggagc cgatcgacgg catcgcgccg cgcgtcttcc
      781 cgcaggtcat cacgcgcacg aaggagctgg agcagagtca tctctgcctc ggcaccaacg
      841 ggtacccgca gaaccacgag gatcgttacg tcacgtacat tctgaacacg gtgctcggcg
      901 ggtcgatgag ctcgcgcctg ttccagaaca tccgcgagaa gcgcgggctg gcgtactcgg
      961 tgttcagcgg cctcagcgcg tatcgcgacg ccgggaacat cacgatttac gcgggctgcg
     1021 cgaccgacgc ggtggacgaa gtggtggatc tgtgcgtcga ggagctccgc ggcatgaagc
     1081 ggacgccggt ggcggaaccg gagctgcgcc gcgccaaaga ccacctgaaa ggcagcctca
     1141 tgctgagcct cgagaacacc gcgagccgca tgtcgcacct cgcacggcag gagatttact
     1201 tcgaccggca tttcggcctg gacgaaacgc tggccggcgt ggagcgcgtc accgccgacg
     1261 acgtccagcg cgtggcgcac gacctgttcg ccaacggctc gctggccgcg accgtgctcg
     1321 ggccgtccgt cccgacgctc acgcgcgagc gcctggatct gacatgattc gccgctacac
     1381 caaccccgag atgggcgcga tctggagcga tcagcgccgc tacgaaacct ggctcgaggt
     1441 cgagctcgcc gccgccgacg ccatggctgc cgcgggcatc gtccctgccg aggccgcgcg
     1501 ggagttgcgc gcgaaggcgt ccttcgacct cgaacgcatc gaggagatcg agcagacgac
     1561 gcagcacgac gtgattgcgt tcacgaccgc ggtggccgaa cgcgtcgggc cgtcggcgcg
     1621 gtggctgcac ttcgggctca cgtcatccga cgtggtggac accgcccagg cgctgcagat
     1681 gcgccaggcg tgcgacctga tcgtcaaggg cctggcgggc ctgatggacg cggtgcgcac
     1741 gcgcgccgag gaacaccggc ggacgccgat gatcggccgc acgcacggcg tgcacgccga
     1801 gccgatgacg ttcggcctca agctggccct gtggtacgcg gagctgcagc gggacgtcga
     1861 tcgcgtcctg cgtgcgcgcg acacgatcgc cgtcggcaag atctcgggcg cggtcggcac
     1921 cttcgcgcac ctggacccgt cgattgaggc agccgtgtgc gagcggctcg ggctgcaggc
     1981 ggcgccggtg tcctcgcagg tgatccagcg cgaccgccac gccgagctcg tcgcggcgct
     2041 ggcgatcacg gcggcgtcgc tcgagaagtt cgcgctcgag atccgcgggc tgcagaagac
     2101 ggaaatcggc gaggtcgagg aaccgttcgg caaagggcag aagggttcgt cggcgatgcc
     2161 gcacaagcgc aacccgatcg gctgcgagca gatcaccggc ctcgcgcggc tgatccgcgg
     2221 gaacgccgtg gcggcgctcg aaaacgtcgc gctctggcac gagcgcgaca tctcgcactc
     2281 gtcggtggag cgcgtcatcc tgcctgacag cttcatcgcg ctcgaccaca tgctgcggcg
     2341 cttcacccgc atcgtccgcg gcatggtcgt caacccgcag cggatgcgcg agaacctgga
     2401 gcgatcgcgc ggcgtcgtct tctcgggcac cgtcctgctg gagctcgcac gccgcggcat
     2461 ctcgcgcgag caggcgtacg agtgggtgca gcgcaacgcg atgcgggcgt ttcacgagca
     2521 gcgcgacttc aagtcgctcc tcctcgccga tcccgacgtg atgcgcgtgc tcacggcgga
     2581 cgagatcgag aaggcgttcg acctggacga ccagttccgg aacgtggatg ccattttcga
     2641 aagggtcttc cggaccgtcc cggccgcggt ataaagaggt gcgatgaaag ccag
//
LOCUS       DASTZR010000247         4328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_431630, whole genome shotgun sequence.
ACCESSION   DASTZR010000247 DASTZR010000000
VERSION     DASTZR010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431630"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2283
                     /locus_tag="VFV20_05525"
     CDS             <1..2283
                     /locus_tag="VFV20_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEU5287844.1"
                     /translation="ALLAERSSFVPGRELAESIVPTSDLRDAERLQDETAAARALLRA
                     QPSAGIGGARDIRDALRRARLGGALDPEQLLMVADTVRAAIKLFEDVHPYPPLAALAR
                     FARPPRPLAEAIEHAVSGTGEVLDRASPRLGGLRAELRAAQARLQQRLDGLLKSSELA
                     RALQEPIVTQRGGRYVVPVKSEMRGAVKGIIHDQSASGATVFIEPLEILEANNALREA
                     ELAERAEVQRILDELSRRVEQDATELDAVTGALARLDLTMAKALLADVLECERPVLDA
                     DGRLDLVAARHPLLVAQGTAVVPIDVRLGTDFRALVVTGPNTGGKTVTLKTIGLLMLM
                     AACGLHIPAQRGSRVPVVKRIFADIGDEQSIAQSLSTFSSHMRNVVATLAEADRGDLA
                     LLDELGAGTDPDEGAALAMAVLETLLARGVLVAATTHYPELKAFALSTPGVSNASVEF
                     DPETLRPTYRLHVGLPGASNAFAIASRLGLDGSVLQRAESHLSELHRSLERTLREAER
                     QRTELAGALDQARIAVEDARRATADAERESAKLRDDAERALRRARAEADELILQARRA
                     LRQAEQARDRAAKRNLVDEAREARESLDAAERAREAVSEPTPVPRPLVPIAVGSPVLV
                     DGVSEPGVLVAVDEKGMADVAAGALRLRVSVASLREAPAPVEAVRSTRPAVSGSAPSV
                     PLQLDLRGARAEEALAVLDRYLNDAAVAGIDRLRIVHGKGTGALRTAVREMLSSHPLV
                     REVTNASPNEGGEGATIVRL"
     gene            complement(2285..2707)
                     /locus_tag="VFV20_05530"
     CDS             complement(2285..2707)
                     /locus_tag="VFV20_05530"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU5287845.1"
                     /translation="MDPSGRVLLVRQRGGPFSGAWLLPGGGVDDGESVEDALRREMRE
                     ETGCELADVRPVATYEVDERTQDFKALVHLYRASALGEPRPEDGSAVRWASPNTNGYH
                     PALRRELADAGLRSEDEDALATAMRATNVAMRRLERKA"
     gene            complement(2771..>4328)
                     /locus_tag="VFV20_05535"
     CDS             complement(2771..>4328)
                     /locus_tag="VFV20_05535"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEU5287846.1"
                     /translation="KQILGGNESAVTRGGFKVITTLDMTKQKAAEQNVKGHVEELHNK
                     NVWNAAMVSIDPRNGEVLAYVGSVDYYNREDPRVQGQFDVAGIGQRQPGSSFKMFNYL
                     TALKKGATPATVVVDARTDFTGRADPSNMNRSTCGYCPENADLQYHGPVTMRQALRES
                     RNVPAVKFLAQYSGIEDTIQTARDMGITTPIDPSQVGLSLTLGAQVVKLLDMTSAYGV
                     LANNGVRVAPTFIQRVEDPRGKVVWAHKDYEQKQAVDKSIAWLMTDILKDMTDPAKDF
                     IFGSWTNIGRPAALKTGTTDNLRDVYSVGYVPQLVTGIWMGNSNNLEMSSVDFSSAMG
                     PGVLWRDYMKEVLADTPKEDWPRPADIVTMNVVVAPGAYGGYGSGLLPGPLSPFSSTE
                     NFIKGKTPARQDDWFVQGCPAPDGTRKVALQLKESAPAMWQRYTDKWAADANAGRHTY
                     SRYSWNVMGSQPCPSPSPSPSPTASPSPSPSPTGSRSPAPTLPPGLTPIPTPTLPPLP
                     TKTPPPGQRP"
BASE COUNT          574 a         1538 c         1577 g          639 t
ORIGIN      
        1 gcgctcctgg cggagcggtc ctcgttcgtt ccgggacgcg agctcgccga gtcgatcgtg
       61 cccacgagcg acctgcgcga cgccgaacgc ctgcaggacg agaccgccgc ggcgcgcgcg
      121 ctgctccgag cgcagccctc ggccggcatc ggcggggcgc gcgacatccg cgacgccctt
      181 cgtcgcgcgc gtctgggtgg ggcgctcgat cccgagcagc tgctgatggt ggccgacacc
      241 gtccgcgcgg cgatcaagct cttcgaggac gtgcacccgt atccaccgct cgccgcgctc
      301 gcgcggttcg cgcgcccgcc gcggccgctc gcggaggcca tcgagcatgc cgtctcggga
      361 acgggcgagg tgctcgaccg cgcgagcccc aggctcggcg ggctgcgcgc ggagctgcgg
      421 gccgcccagg cgcgcctgca gcagcgcctc gacgggctcc tcaagtcgag cgagctcgcg
      481 cgagcgctcc aggagcccat cgtcacgcag cgcggcggcc gctacgtcgt gccggtgaag
      541 tcggagatgc gcggggcggt caagggcatc atccacgacc agtccgcgag cggcgcgacg
      601 gtgttcatcg agccgctcga gatcctcgag gcgaacaacg ccctgcgcga ggccgagctc
      661 gcggagcggg ccgaggtgca gcggatcctc gacgagcttt cgcgacgcgt cgagcaggac
      721 gcgaccgagc tcgacgccgt gaccggcgcg ctcgcgcgcc tcgatctcac gatggcgaag
      781 gcgctcctcg cggacgtcct ggaatgcgag cgcccggtgc tcgacgccga cggacggctc
      841 gacctcgtcg cggcgcggca tccgctgctc gtggcgcagg ggaccgccgt ggtccccatc
      901 gacgtgcggc tcggcacgga cttccgcgcg ctcgtcgtga cgggcccgaa cacgggcggc
      961 aagaccgtga cgctgaagac gatcggcttg ctcatgctca tggctgcctg cggcctgcac
     1021 atcccggcgc agcgcgggag ccgtgtgccc gtcgtgaagc ggatcttcgc cgacatcggc
     1081 gacgagcagt ccatcgccca gtcgctctcg acgttcagct cacacatgcg caacgtcgtc
     1141 gccacgctgg ccgaggccga ccgcggcgat cttgccctcc tcgacgagct cggcgccggc
     1201 accgaccccg acgagggcgc ggcgctcgcg atggccgtcc tcgagacgct gctcgcgcgg
     1261 ggcgtcctgg tcgccgcgac gacgcactat ccggagctca aggcgttcgc cctcagcacg
     1321 ccgggcgtct cgaacgcgag cgtcgagttc gatccagaga cgctgcgccc cacgtaccgc
     1381 ctccacgtcg ggctgcccgg cgcgtcgaac gcgttcgcca tcgcctcgcg cctcggtctc
     1441 gatggaagcg tgctgcagcg cgcggagtcg cacctctcgg agctgcatcg ctccctggag
     1501 cggacgctgc gcgaggccga gcggcagcgc accgagctcg cgggcgcgct ggaccaggcg
     1561 cgcatcgcgg tcgaggacgc gcggcgggcg accgcggacg cggagcgcga gagcgcgaag
     1621 ctgcgcgacg acgccgagcg cgcgctccgc cgcgcccgtg ccgaggccga cgagctgatc
     1681 ctgcaggcga ggcgcgcgct caggcaggcc gagcaggcgc gtgaccgcgc ggcgaagcgc
     1741 aacctcgtgg acgaggctcg cgaggcgcga gagtcgctcg acgccgccga gcgcgcgcgc
     1801 gaggccgtca gcgagccgac gcccgtgccg cgtccactcg tcccgatcgc cgtcggatcg
     1861 cccgtcctcg tggacggagt gagcgagccc ggcgtgctcg tcgccgtgga cgagaagggc
     1921 atggccgacg tcgcggcggg ggcgctccgg ctgcgcgttt ccgtggccag cctgcgcgag
     1981 gcgcccgcgc ccgtcgaggc cgtgcgctcg acccgccccg cggtgagcgg cagcgcgccg
     2041 agcgtgccgc tgcagctgga tctgcgcggc gcccgcgcgg aggaggcgct cgccgtgctc
     2101 gaccgctacc tcaacgacgc cgcggtcgcg gggatcgacc ggctgcgcat cgttcacggc
     2161 aagggcaccg gtgcgctccg caccgccgtg cgcgagatgc tgtcgtcgca tccgctggtg
     2221 cgcgaggtca cgaacgcgtc accgaacgag ggcggggaag gggcgacgat cgtccggctc
     2281 taggtcatgc ctttcgctcg agccggcgca tcgcgacgtt ggtcgcgcgc atcgcggtcg
     2341 cgagcgcgtc ctcgtcctcg gaccgcaggc ctgcgtcggc gagctcacga cggagggcgg
     2401 ggtggtagcc attcgtattc ggtgacgccc agcgcacggc cgacccgtcc tcagggcgcg
     2461 gctcgccgag cgccgacgcg cgatataggt gcaccagcgc cttgaagtcc tgcgtgcgct
     2521 cgtcgacctc gtacgtcgcg accggccgca cgtcggcgag ctcgcacccc gtctcctcgc
     2581 gcatctcccg tcgcagcgcg tcctcgaccg actcgccgtc gtccacacca ccgccgggga
     2641 gcagccatgc gccgctgaac ggaccgccgc gctggcgcac gaggaggacg cggcccgacg
     2701 ggtccacgca cgcgacgatg gcggtgacgc gcctctcctc gttgacgggg aaagggtaag
     2761 ggcgctcggc ttagggacgc tgaccgggcg gcggtgtctt cgtcggcagg ggcggaagcg
     2821 tcggcgtcgg gatgggcgtg agcccgggcg gcagcgtcgg ggccggggat cgcgagccgg
     2881 tcggcgacgg cgacggcgac ggagatgccg tcggggaggg ggagggtgac gggctggggc
     2941 agggctgcga gcccatgacg ttccacgagt accggctgta cgtatggcga ccggcgttcg
     3001 cgtcggcggc ccacttgtcg gtgtagcgct gccacatcgc cggcgcgctc tccttgagct
     3061 gcagcgcgac cttgcgcgta ccgtcgggcg ccgggcaccc ctgcacgaac cagtcatcct
     3121 gccgcgccgg cgtcttgccc ttgatgaagt tctcggtgga cgagaagggg gagagcgggc
     3181 caggcagcag cccggatccg tacccgccgt aggcgcccgg tgcgacgacg acgttcatcg
     3241 tcacgatgtc ggccggccgc ggccagtcct ccttcggcgt gtccgcgagc acttccttca
     3301 tgtagtcgcg ccacagcacg ccgggaccca tcgcgctcga gaagtcgacg gacgacatct
     3361 cgaggttgtt gctgttgccc atccagatgc ccgtgacgag ctggggcacg tagccgacgc
     3421 tgtacacgtc gcggaggttg tcggtggtcc cggtcttcag ggccgcgggc cgcccgatgt
     3481 tggtccagct gccgaagatg aagtccttcg ccgggtcggt catgtccttg aggatgtcgg
     3541 tcatgagcca cgcgatggac ttgtccacgg cctgcttctg ctcgtagtcc ttgtgcgccc
     3601 acaccacctt gccgcgcggg tcctcgaccc gctggatgaa ggtcggcgcc acgcggacgc
     3661 cgttgttcgc cagcacgccg taggcgctcg tcatgtcgag gagcttcacg acctgcgcgc
     3721 cgagcgtcag cgacagaccg acctgcgacg gatcgatcgg cgtcgtgatg cccatgtcgc
     3781 gggcggtctg gatggtgtcc tcgatgccgc tgtactgcgc gaggaacttc acggcgggca
     3841 cgttgcgcga ctcgcgcagt gcctgccgca tggtgaccgg tccgtggtac tgcaggtccg
     3901 cgttctccgg acagtagcca caggtgctgc ggttcatgtt gctcgggtca gccctgccgg
     3961 tgaagtccgt ccgcgcgtcc acgaccaccg tcgcgggcgt ggcgcccttc ttgagcgcgg
     4021 tgaggtagtt gaacatcttg aaggacgagc ccggctgccg ctggccgatg ccggcgacgt
     4081 cgaactgccc ctgcacacga gggtcctcgc ggttgtagta gtcgaccgaa ccgacgtacg
     4141 cgagcacctc gccgttgcgc ggatcgatgc tgaccatggc ggcgttccac acgttcttgt
     4201 tgtggagctc ctcgacgtgg cccttcacgt tctgctcggc ggccttctgc ttggtcatgt
     4261 cgagcgtggt gatcaccttg aagccgccgc gggtcacggc cgattcgtta ccgccgagga
     4321 tctgcttc
//
LOCUS       DASTZR010000248         2011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1267180, whole genome shotgun sequence.
ACCESSION   DASTZR010000248 DASTZR010000000
VERSION     DASTZR010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267180"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VFV20_05540"
     CDS             <1..658
                     /locus_tag="VFV20_05540"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEU5287847.1"
                     /translation="AAALAPYTAVCTAAAAAASPLLRNVGTVGGNLCQHTRCWYYRGA
                     EWHCWLGGGETCYAQIGDHRKHGLEEGDCISAHPSDLAPALAATGATVELRSRDGARE
                     LPLLDLYRRPTADNRALVVLEPGEVVVSVRLPAPPDASAYERLGERAAFSFPLVSVAA
                     ARRGDSVRVVAAGVANIPLGLDPSDPLAELPGNPQSAWKRRALATLVERAVTAVAVES
                     "
     gene            complement(659..1813)
                     /locus_tag="VFV20_05545"
     CDS             complement(659..1813)
                     /locus_tag="VFV20_05545"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5287848.1"
                     /translation="MDPIARANGLLEQYARRTLDELPRDTPIFDAHIHLGTDIDGMVG
                     DRDELLGIQRAYGVGRSFVFCLDEPDRHPSFSAANDRTLAHARAAPDELLPFVRLDLD
                     ERPIEEATRCLGLGACGIKLHPRAQRFLPDDPRLEPVFALAAARRVPILIHGGRGLPP
                     IADSLGRLLDRHAPPALIVAHAGIVDLAAMARNFAGRPGVFFDTSVWSPLDLLDLYRL
                     VPPEQVVYASDYPYGRQPNSLLMAVRTARASGFDDRQLRALLHDTAAGIADGAEPVRP
                     SAPQGAAELSHPVTFLRIHQYLTMASAMLWMRHPADTYGVIGLALNACDERSNGYRAQ
                     TEQIRELLVVTRDLWELASQAEDETGRRRAQRGAFRLVHLATVLAVTNAG"
BASE COUNT          284 a          714 c          749 g          264 t
ORIGIN      
        1 ggcagccgcg ctcgcgccgt acacggccgt ctgcaccgcc gctgccgccg cggcgtcgcc
       61 gctgcttcgc aacgtcggca ccgtgggtgg gaacctctgc cagcacacgc gctgctggta
      121 ctaccggggc gcggagtggc actgctggct cggcggcggc gagacctgct acgcgcagat
      181 cggcgaccac cgcaagcacg ggctcgagga gggagactgc atctccgccc atccgtccga
      241 cctcgcgcct gcgctcgcag ccaccggcgc aacggtagag ctccgctcgc gagacggcgc
      301 ccgcgagctg ccgctgctcg acctgtaccg gcggcccacg gcggacaacc gcgcgctcgt
      361 cgtgctcgag ccgggcgagg tcgtcgtctc ggtccggctt cccgcgccgc ccgacgcatc
      421 cgcctacgag cggttgggcg agcgcgccgc cttctcgttc ccgctggtct cggtcgccgc
      481 tgcgcgccgg ggggacagcg tgcgggtcgt tgcggcaggc gtcgccaaca tcccactcgg
      541 gctcgacccg tccgatccgc tcgcggagct gccggggaac ccgcagtccg cgtggaagcg
      601 acgcgcactg gcgacgctcg tcgagcgcgc ggtcaccgcg gtcgcggtcg agagctgatc
      661 agccggcgtt cgtgaccgcg agcacggtcg cgaggtggac gaggcggaac gcaccgcgct
      721 gggcacgccg ccgtcccgtc tcgtcctccg cctgagacgc gagctcccac aggtcgcgcg
      781 tgacgacgag gagctcgcgg atctgctccg tctgcgcgcg gtacccgttc gaccgctcgt
      841 cgcaggcgtt gagcgcgagc ccgatcacgc cgtacgtgtc ggccggatgg cgcatccaca
      901 gcatcgccga ggccatcgtc agatactggt ggatgcgcag gaaggtgacg ggatgcgaga
      961 gctccgccgc accctgcggc gcactcggcc gcacgggctc ggcgccgtct gcgatccccg
     1021 ccgcggtgtc gtggagcagc gcccgaagct ggcgatcgtc gaagcccgac gcgcgcgccg
     1081 tgcgcaccgc catgagcagc gagttcggct ggcggccgta cgggtagtcg gatgcgtaga
     1141 cgacctgctc cggcgggacg aggcggtaga ggtcgagcag gtcgagcggg ctccagacgg
     1201 atgtgtcgaa gaacacaccc ggccggcccg cgaagttccg ggccatcgcg gcgaggtcga
     1261 cgatccccgc gtgcgcgacg atcagcgcgg gcggcgcgtg tcggtcgagc agccgtccga
     1321 gagagtctgc gatcggcggc aggccgcgtc cgccgtggat caggatcggg acgcggcgcg
     1381 cggccgcgag cgcgaacacc ggctcgagcc gaggatcgtc cggaaggaag cgctgagccc
     1441 gagggtgaag cttgattccg catgcgccga ggccgagaca ccgcgtcgcc tcctcgatgg
     1501 gacgctcgtc gagatcgagc cgcacgaacg gcagcagctc gtcgggcgct gcgcgggcgt
     1561 gggcaagcgt ccggtcgttc gcggcgctga aggacggatg acggtcgggc tcgtcgaggc
     1621 agaacacgaa cgaacggccg actccgtacg cgcgctggat cccgagcagc tcgtcgcggt
     1681 cgccgaccat cccgtcgatg tcggtgccga ggtggatgtg cgcatcgaag atcggcgtgt
     1741 cacgcggcag ctcgtcgagc gtccgccgcg cgtactgctc gagcaggccg tttgcgcgcg
     1801 cgatggggtc cacgcggtga gtgtacggct gcctcagtcc gtcgcggccg cgagcacgcg
     1861 gtcgacgaac gccggctccg gcaccgaacc ggcccacgcg tagcgatcgt cgacgacgat
     1921 cgccggcacg gcatgaactc cgtggcggtc ggcctcgacc gcgaactccg aggcctcgat
     1981 cgcacgcgcg cgaatgcgcg gggaggcgag c
//
LOCUS       DASTZR010000249         2334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_927942, whole genome shotgun sequence.
ACCESSION   DASTZR010000249 DASTZR010000000
VERSION     DASTZR010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927942"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1042)
                     /locus_tag="VFV20_05550"
     CDS             complement(89..1042)
                     /locus_tag="VFV20_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU5287849.1"
                     /translation="MRNATLRQLRVFAAAARHQSFGKAALELHLTPPAVSMQIRELEE
                     QVGLPLFDRSGRQVALTVTGEYLLVYVRKVLATLKDAEDAIARFRGLETGRVSIGMVS
                     TAKYYVPRMLAQFRQEHPAIDVKLAVGGNREQLVALLQANEVDLAIMGRPPRELATRA
                     EPFAVHPHAIVTAPDHPLTRIGHAPASLLANYEFIIRESGSGTRAAMEKYFADHRLQP
                     RVAMEMASNETIKQAVMAGMGVSFLSLHTIGLERRAGLLAVPEIEGLPVLKRWYVVNN
                     LSKTLSPAAEAFRYFILEEGERFLAKEYGAPAPAADAREPA"
     gene            1147..>2334
                     /locus_tag="VFV20_05555"
     CDS             1147..>2334
                     /locus_tag="VFV20_05555"
                     /EC_number="4.1.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-bisphosphate carboxylase large subunit"
                     /protein_id="HEU5287850.1"
                     /translation="MNKPVEGPIRDTKERYKGGVLKYRQMGYWRPDYEPSDGDVICVF
                     RITPQDGVEPEEAAAAVAGESSTATWTVVWTDLLTAHESYQAKAYRVDPVPGTGAGTG
                     REAQYFAYIAYDLILFEEGSIANMTASLIGNVFSFKPLKAARLEDIRIPPAYVKTFKG
                     PPTGIVVERERLDKYGRPLLGATMKPKLGLSGKNYGRVVYEALTGGLDFTKDDENINS
                     QPFMHWRDRFLNCMEAVNKAQAGSGEIKGHYLNVTAATMEEMYERAEFAKELGSNILM
                     IDLVIGWTAIQSMSNWCRRNDMILHMHRAGHGTYTRQKNHGVSFRVIAKWLRLAGVDH
                     LHAGTAVGKLEGDPMTVQGYYNVCRDSYTRQDLPRGLFFDQDWADMKKVMPVASGGIH
                     AGQM"
BASE COUNT          424 a          769 c          789 g          352 t
ORIGIN      
        1 gagctacgaa ctcgacaaga agaagatgta cttcccgcgc tagcgggaac gccgacggag
       61 agcgattcct tcgggcggca ccgggccctc acgccggctc gcgcgcatcg gccgcgggcg
      121 caggcgcgcc gtactccttc gccaggaagc gctcgccttc ctcgaggatg aagtagcgga
      181 acgcctcggc ggccggggac agcgtcttcg acaggttgtt gacgacgtac cagcgtttca
      241 gtaccggcag gccttcgatc tccggcacgg cgagcagtcc cgcgcggcgc tcgaggccga
      301 tcgtgtgcag cgacaggaag ctcacgccca tgcctgccat cacggcctgc ttgatcgtct
      361 cgttgctcgc catctccatc gccacgcgtg gctgcaggcg gtgatcggcg aagtactttt
      421 ccatcgcggc gcgggtcccg gagccggact cgcggatgat gaattcgtag ttggcgagca
      481 ggcttgccgg cgcgtggccg atgcgcgtca gcgggtgatc gggcgccgtg acgatcgcgt
      541 gcgggtgcac ggcgaacggc tcggcacgcg tggccagttc gcgcggcggc cggcccatga
      601 tcgcgaggtc gacctcgttg gcctgcagga gcgcaaccag ctgttcccgg ttgccgccga
      661 cggcgagctt cacgtcgatc gccgggtgct cctggcggaa ctgcgccagc atccgcggca
      721 cgtagtactt ggcggtgctg accatcccga tcgagacccg ccccgtttcc agtccgcgga
      781 accgggcaat ggcgtcctcg gcatccttca aggtcgcgag caccttgcgg acgtacacga
      841 gcaggtactc gcccgtgacg gtgagcgcga cctgccgccc ggagcggtcg aacagcggca
      901 gccccacctg ctcctccagc tcccggatct gcatcgacac cgccggcggc gtcaggtgca
      961 gctccagcgc cgccttgccg aagctttggt gccgggccgc ggcggcgaag acgcgcagct
     1021 gccgcaatgt cgcgtttcgc ataagcaaaa cttaactgaa agatcagaag agttgactgt
     1081 accttaaatg tcaccctccc tagagtccgg cttcacgcgc gacgaggcgc tgccacagga
     1141 ggcgggatga acaagccggt cgaaggaccg atccgggaca cgaaggagcg ctacaagggt
     1201 ggcgtcctga agtaccggca gatgggctac tggcgcccgg actacgagcc cagcgacggc
     1261 gacgtgatct gcgtgttccg gatcacgccg caggacggcg tggaaccgga ggaggcggcc
     1321 gcggccgtgg cgggcgagtc gtcgacggcg acctggaccg tcgtctggac cgacctgctg
     1381 acggctcacg agtcgtacca ggcgaaggcg tatcgcgtcg accccgtccc gggaaccggt
     1441 gccggcaccg gccgcgaggc gcagtacttc gcctacatcg cgtacgacct gatcctgttc
     1501 gaagagggct cgatcgccaa catgacggcg agcctgatcg gcaacgtctt cagcttcaag
     1561 ccgctgaagg ccgcgcggct cgaggacatc cgcattccgc ccgcgtacgt caagaccttc
     1621 aagggcccgc cgaccgggat cgtggtcgag cgcgagcgcc tcgacaagta cggccgtcca
     1681 ctgctcggcg cgacgatgaa gcccaagctc gggttgtccg gaaagaacta cgggcgcgtc
     1741 gtctacgagg cgctgaccgg cggactcgac ttcacgaagg acgacgagaa catcaactcg
     1801 cagcccttca tgcactggcg cgaccgcttc ctcaactgca tggaagcggt caacaaggcg
     1861 caggccggga gcggtgagat caagggccac tacctcaacg tcaccgcggc gacgatggag
     1921 gagatgtacg agcgcgccga gttcgcgaag gaactgggct cgaacatcct gatgatcgac
     1981 ctcgtgatcg gctggaccgc gatccagtcg atgtccaact ggtgcaggcg gaacgacatg
     2041 atcctgcaca tgcaccgcgc ggggcacggg acgtatacgc ggcagaagaa ccacggtgtc
     2101 agcttccgcg tgatcgccaa gtggctgcgg ctggcgggcg tggaccacct gcacgcgggc
     2161 acggccgtcg gcaagctgga aggcgacccg atgacggtgc agggctacta caacgtctgc
     2221 cgcgacagct acaccaggca ggacctgccg cgcggcctgt tcttcgacca ggactgggcc
     2281 gacatgaaga aggtcatgcc ggtcgcctcc ggcggcatcc acgccggcca gatg
//
LOCUS       DASTZR010000250         3340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_118203, whole genome shotgun sequence.
ACCESSION   DASTZR010000250 DASTZR010000000
VERSION     DASTZR010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118203"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VFV20_05560"
     CDS             complement(<1..556)
                     /locus_tag="VFV20_05560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018513667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5287851.1"
                     /translation="MAPAQGGEPLLDVVIPVYNEERDLEPSVRRLHEHLTRSMPFPSV
                     ITIADNASRDGTLAVAERLAADLPGVRVLHLDAKGRGRALRAAWLKSDAPIVAYMDVD
                     LSTDLKALLPLVAPLVSGHSDIAIGSRLARGARTHRGPKRELISRAYMLILRLALGAG
                     FTDAQCGFKAVRTSVARDLVSRIED"
     gene            complement(625..1125)
                     /locus_tag="VFV20_05565"
     CDS             complement(625..1125)
                     /locus_tag="VFV20_05565"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEU5287852.1"
                     /translation="MLVPCTVESVRVHVLTGQHVVLLQAKDGNRLLPIWIGPDQAHSI
                     ATRIAGIASERPLTHDLFMDVVGKLGAEITRIVVKELVSDDSGGGIFHGSLFLQLGEK
                     EVEIDCRPSDAIALAVRCDARILVADTVLDRSAILAESEEDQNLTVFKEFIESLPDDP
                     QAPPTN"
     gene            complement(1135..1836)
                     /locus_tag="VFV20_05570"
     CDS             complement(1135..1836)
                     /locus_tag="VFV20_05570"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU5287853.1"
                     /translation="MIPLHDSSPPRRVPVVTRLLLFANVALWLYVVYLSRDPGAMNAF
                     FDRYSFDWSAFTAALTSGRWTLDTFIPVLAHMFLHGGWLHVLGNMLYLWIFGDNVEDA
                     MGSGPFLVFYVAAGIIAAIGQGLVAPAPMVGASGAIAGVLGAYLVMYPTNRISTLVFL
                     GIFISVFEIPAIIVIGMFIVLQVIEGVAELRMSLHPATQGVAYFAHVFGFLAGILLLP
                     VFRGRGSRHARIGWR"
     gene            complement(1856..>3340)
                     /locus_tag="VFV20_05575"
     CDS             complement(1856..>3340)
                     /locus_tag="VFV20_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287854.1"
                     /translation="LRSAAVTLHPRAAAEHVHNRAVADGLVGSDGAIFVEITQSSGAA
                     ARLAPEPAPVRQPEEVVIAESIRSRIDAVWRRRPRIGGIARAAAAPVASAATKGVAVG
                     LELMPKRGVGLPRHPDTARERSRRQRRAVTTLAILLLLAATGVGALAYRDYESTRAQR
                     EYQLAVLSAEDSLASAHRLAERKPPDPDGARTRIAQMRVKITEALRYPAADKEHLAAL
                     GTDADVLTDRLDGVLLDLARIAPGAKPTQIVGNANGLYVADPGSGRLWRIFGDPLQNG
                     IVMQRGARGVGAPRQVTWQDEVLYAFDDARKLWRAEGDQVRDVTPADKEVWKDVTAVA
                     VFTSNLYVLDAASGQLWKHESSDGVTFGKGAGYLAAVVPANTARSLAIDGDVWIVTST
                     SDILRFRRNPLVTTASRVDFTPRWQGDAPHPAAIQAVSGQTNIYVLDPVGRTVVQLAR
                     DGRELLRIALPPTLPEASAFYVSEASRVAYTVHGSKIVATNLDR"
BASE COUNT          516 a         1123 c         1217 g          484 t
ORIGIN      
        1 ggtcctcgat ccgcgagacg agatcccgcg ccaccgacgt ccgcacggcc ttgaagccgc
       61 actgcgcgtc agtgaagccc gcacccagcg cgagacgcag gatgagcatg tacgcgcgcg
      121 agatgagctc ccgcttcgga ccgcgatgcg tgcgcgcgcc acgcgcgagg cggctgccga
      181 tcgcgatgtc gctgtggccg gagacgagcg gcgcgaccag cggcaggagc gccttgaggt
      241 cggtggacag atcgacgtcc atgtacgcca cgatgggcgc gtcgctcttg agccacgccg
      301 cacggagcgc acggccgcgc cccttcgcgt ccagatggag cacccgcacg cccgggagat
      361 cggcggccag acgctccgcg acggcgaggg tgccgtcgcg gctcgcgttg tcggcgatcg
      421 tgatgaccga tgggaacggc atcgaccgcg tcaggtgctc gtgaaggcgg cggacgctcg
      481 gctcgaggtc gcgttcctcg ttgtagacgg ggatgacgac gtccagcagc ggctcgccgc
      541 cctgcgcagg ggccatgggt atcacggaca cggccacatc ctgcgtcatc gcgccgctgt
      601 catgcacggg agcgaggcgg gccgctagtt ggtcggcggc gcctgggggt catccgggag
      661 cgattcgatg aactccttga acaccgtcag gttctggtcc tcctcgctct cggcgaggat
      721 cgcggagcgg tcgagcaccg tgtcggccac gaggatccgt gcgtcgcagc ggaccgcgag
      781 cgcgatcgcg tccgaggggc gacagtcgat ctcgacctct ttctcgccga gttggaggaa
      841 gaggctgccg tggaagatgc caccgccgct gtcatccgag acgagctcct tgacgacgat
      901 gcgcgtgatc tcggcgccga gcttgccgac cacgtccatg aagagatcgt gcgtgagcgg
      961 ccgttcgctg gcgatgccgg cgatgcgggt ggcgatgctg tgcgcctgat cgggcccgat
     1021 ccagatcggg aggaggcggt tgccgtcctt cgcctgcagc agcacgacgt gctgacccgt
     1081 gagcacgtgc acgcggaccg actcgaccgt gcagggcacg agcatcgtct aaggctacct
     1141 ccagccgatg cgcgcgtggc gtgacccgcg gccgcggaat accggcagta agaggatgcc
     1201 tgccaggaag ccgaacacgt gggcgaagta cgcgacgccc tgcgtcgcgg gatggaggct
     1261 catgcgcagc tcggcgacac cctcgatgac ctgcagcacg atgaacatgc cgatgacgat
     1321 gatcgcgggg atctcgaaca cgctgatgaa gatgccgagg aacaccagcg tcgaaatgcg
     1381 attcgtcgga tacatcacga ggtacgcgcc gagcacgccg gcgatcgcgc cggatgcgcc
     1441 gaccatgggc gcgggggcca cgaggccctg accgatcgcg gcgatgatgc ccgcggcgac
     1501 gtagaagacc aggaagggac cgctacccat cgcgtcttcc acgttgtcgc cgaagatcca
     1561 gagatacagc atgttgccga gcacgtggag ccagccgccg tgcaggaaca tgtgcgcgag
     1621 gaccgggatg aacgtgtcga gcgtccaccg accgctcgtg agcgccgcgg tgaacgcgct
     1681 ccaatcgaac gagtagcggt cgaagaacgc gttcatcgcg cccgggtctc gcgagaggta
     1741 gacgacgtag agccacagcg ccacgttcgc gaacagcagc agccgcgtga cgacgggcac
     1801 gcggcgcgga ggactcgagt cgtggagcgg gatcacgatg cgctcgggtc ggcgatcatc
     1861 ggtcgaggtt cgtcgccacg atcttcgagc cgtgcaccgt gtacgccacg cgcgacgcct
     1921 cggagacgta gaaggcggat gcctcgggca gggtcggcgg gagcgcgatc cgcagcagct
     1981 cgcgcccgtc ccgcgcgagc tgcaccaccg tgcgcccgac cgggtcgagg acgtagatgt
     2041 tcgtctggcc gctcacggcc tggatcgccg ccggatgcgg cgcgtcgccc tgccaccgag
     2101 gcgtgaagtc cacacgcgag gccgtcgtca cgagcgggtt ccggcggaag cgcaggatgt
     2161 ccgacgtgct cgtgacgatc cacacgtcgc cgtcgatggc gagggagcgc gccgtgttcg
     2221 ccgggacgac cgccgcgagg tagccggcgc ccttcccgaa ggtgacgccg tcgctcgatt
     2281 cgtgcttcca cagctgtccc gaggccgcgt cgaggacgta gagattggag gtgaagacgg
     2341 cgaccgcggt cacgtccttc cacacctcct tgtcggccgg cgtgacgtcc cgcacctggt
     2401 cgccctcggc gcgccagagc tttcgcgcat cgtcgaaggc gtagagcacc tcgtcctgcc
     2461 acgtcacctg acgcggcgca ccgaccccgc gcgctccgcg ctgcatgacg atgccgttct
     2521 gcagcgggtc gccgaagatc cgccagagcc ggcccgagcc cggatcggcg acgtagagtc
     2581 cattggcgtt gccgacgatc tgcgtgggct tcgccccggg cgcgatgcgc gcgaggtcga
     2641 gcagtacgcc gtcgaggcgg tccgtgagca cgtccgcgtc cgttcccagc gccgcgaggt
     2701 gttccttgtc ggcggcggga tagcggagcg cctcggtgat cttcacgcgc atctgcgcga
     2761 tcctggtgcg cgcgccgtcc ggatccggtg gcttgcgttc ggccagccgg tgcgcggacg
     2821 ccaggctgtc ctcggcggag agcaccgcga gctggtactc gcgctgcgct cgtgtcgatt
     2881 cgtagtcgcg gtaggcgagc gcgccgacgc cggtcgccgc gagcaggagg aggatcgcca
     2941 gcgtggtgac ggcgcgccgc tgccggcgcg atcgctcgcg cgcggtatcg ggatgccgtg
     3001 ggagtccgac gccacgcttc ggcatgagct cgagccccac cgcgacgccc ttcgtggccg
     3061 ccgacgccac gggtgccgcg gcggccctcg cgatcccgcc gatgcgcggc cgccgtcgcc
     3121 agacggcgtc gatgcgtgag cggatcgatt cggcgatgac gacctcttcc ggctggcgca
     3181 ccggggccgg ttccggcgcg agccgcgcgg cggcgccgga cgattgcgtg atctcgacga
     3241 agatggctcc gtcgctgccg acgaggccgt cagcgaccgc gcggttgtgc acgtgttccg
     3301 cggcggcgcg cgggtggagc gtcaccgccg cgctgcgcag
//
LOCUS       DASTZR010000251         2488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_402709, whole genome shotgun sequence.
ACCESSION   DASTZR010000251 DASTZR010000000
VERSION     DASTZR010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402709"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1692
                     /gene="acs"
                     /locus_tag="VFV20_05580"
     CDS             <1..1692
                     /gene="acs"
                     /locus_tag="VFV20_05580"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEU5287855.1"
                     /translation="SGDKVAYHWIGEDDTRRDVTYAELLEMTQRFANVLKKLGVEKGD
                     VVGIYMPMIPETPAAMLACARIGATHNVVFGGFSVEAVRERMEVSDAKVLVTCNTSLR
                     RGKPMPMKSNLDEVVDGLPKLEHVVVVKRTDDDCEMKDGRDIWWHEACEEVDAECPAE
                     PLDAEHPLFILYTSGSTAKPKGILHTTGGYLTGVYATTQLVFDLKPESDVYWCAADIG
                     WVTGHSYIVYGPLANGITSVMYEGAPDYPDKDRWWSIVEEQKVTIMYTAPTAIRACIK
                     WGREYPEKHDLSSLRLLGSVGEPINPKAWIWYHKVIGGERCPIVDTWWQTETGHILIT
                     PLPGITETKPGSATKAFPGIEAAVVNKDGEVVDEGGGFLSLRRPWPGMLRTLFKEDER
                     YVETYWSKWGKDVYVVGDAARIDEDGYFWIVGRTDDVVNVSGHRMSTMEVESAIVSHD
                     RVAEAAVIGQADEDTGQAIVAFVTLEGGAKEGEDNESLDEALREHVAQKIGKLARPKR
                     FVYADDLPKTRSGKIMRRLLRDIAEGRELGDVTTLRDPAVTEMIQDRMSSGDGED"
     gene            1728..>2488
                     /locus_tag="VFV20_05585"
     CDS             1728..>2488
                     /locus_tag="VFV20_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287856.1"
                     /translation="MRGRLPLLILVASLALPAPAFAGRLIVTGHDADRRCALLDQQCG
                     FLKATIKYVRDTAPNAKRPVLVLDRGPKQLAVAMQKAWSTTGSYTGPKLRVVDPRSPT
                     FSNLTIDVKHFSTIAIASDSSCGGCDLEWQDAQAIWKRHAVIQKFLNQGGGIFAGAGG
                     TNAAAYYRFVPVPGAGPASEGPFQVTAYGKTIGLKDADLSGAVANTFAPPDVGRMDVA
                     AMNANKVGDTLIADGRVRKGKLIADTTIPPDIGQS"
BASE COUNT          465 a          827 c          843 g          353 t
ORIGIN      
        1 tcgggcgaca aggtcgccta ccactggatc ggcgaggacg acacacgccg tgacgtcacc
       61 tacgcggagc tgctcgagat gacgcagcgc ttcgcgaacg tgctgaagaa gctcggggtc
      121 gagaagggcg acgtggtcgg catctacatg ccgatgattc cggagacccc ggcggcgatg
      181 ctcgcgtgcg cgcggatcgg cgcgacccac aacgtcgtct tcggcggctt ctcggtcgag
      241 gctgtgcgcg agcgcatgga ggtctcagac gcgaaggtgc tcgtcacctg caacacctcg
      301 ctgcgccgcg gcaagccgat gccgatgaag tcgaacctcg acgaggtcgt cgacgggctg
      361 cccaagctcg agcacgtcgt ggtcgtcaag cggacggacg acgactgcga gatgaaggac
      421 ggccgcgaca tctggtggca cgaggcctgc gaggaggtcg acgccgagtg cccagccgag
      481 ccactcgatg ccgagcaccc gctcttcatc ctctacacgt cgggatcgac cgcgaagccg
      541 aagggcatcc tgcacacgac cggcggctac ctgaccggcg tctacgcgac gacccagctc
      601 gtcttcgacc tcaagccgga atctgacgtc tactggtgcg cggccgacat cggctgggtc
      661 accggccact cctacatcgt ctacggcccc cttgcgaacg ggatcacgag cgtgatgtac
      721 gagggcgcgc ccgactaccc ggacaaggac cgctggtggt cgatcgtcga ggagcagaag
      781 gtgacgatca tgtacaccgc tccgaccgcg atccgcgcat gcatcaagtg gggtcgcgaa
      841 taccccgaga agcacgacct gagctcgctg cgcctgctcg gctcggtcgg cgagccgatc
      901 aacccgaagg cgtggatctg gtaccacaag gtgatcggcg gcgagcgctg cccgatcgtc
      961 gacacgtggt ggcagaccga gaccggtcac atcctgatca cgccgctgcc ggggatcacc
     1021 gagaccaagc cgggctcggc gacgaaggca tttccgggta tcgaagccgc agtcgtcaac
     1081 aaggatggtg aggtcgtcga cgagggcggc ggattcctca gcctgcgccg gccgtggcca
     1141 ggcatgctgc ggacgctgtt caaggaggac gagcgctacg tcgagaccta ctggtcgaaa
     1201 tggggcaagg acgtctacgt ggtcggcgac gctgcgcgca tcgacgagga cggctacttc
     1261 tggatcgtcg ggcgcaccga cgacgtggtg aacgtctccg ggcaccgcat gtcgacgatg
     1321 gaggtcgagt cggcgatcgt gtcccacgac cgtgtcgccg aggcggccgt gatcggtcag
     1381 gccgacgaag acacgggtca agcgatcgtc gcgttcgtca cgctcgaagg cggcgcgaag
     1441 gagggcgagg acaacgagtc gctcgacgaa gcgctgcgtg agcacgttgc ccagaagatc
     1501 ggcaagctcg cgcgcccgaa gcggttcgtc tacgccgacg acctgccgaa gacgcggtcg
     1561 ggcaagatca tgcgccggct cttgcgcgac atcgcggagg gccgtgagct gggcgacgtg
     1621 acgaccctgc gcgaccctgc cgtcaccgag atgatccagg accgaatgag ctcgggcgac
     1681 ggcgaggatt aagcccagat tcaggaataa ggacgcatac tcggagggtg cgaggacggc
     1741 tccccctcct catcctcgtc gcgtcgctgg cactgcccgc gcccgccttc gcgggccggc
     1801 tgatcgtgac cggacacgac gccgaccgcc gctgtgccct gctcgaccag cagtgcggct
     1861 tcctcaaggc aacgatcaag tacgtacgcg acaccgcgcc gaacgcgaag aggccggtgc
     1921 tggtcctcga ccgcggtccc aagcagctcg cggtggcgat gcagaaggcg tggagcacga
     1981 ccggctccta caccggcccg aagctgcgcg tggtcgatcc gcgctcgccg acgttcagca
     2041 acctgacgat cgacgtcaag cacttcagca cgatcgcgat cgcgtccgac tcgagctgcg
     2101 gcggctgcga tctggaatgg caggacgcgc aggcaatctg gaagcgccac gcggtcatcc
     2161 agaagttcct caaccagggc ggcgggatct tcgccggcgc cggcggcacg aacgccgcgg
     2221 cttactaccg cttcgtgccc gtccccggcg cgggacccgc aagcgagggg ccgttccagg
     2281 tgaccgccta cggcaagacc atcggcctca aggacgccga cctgtccggt gccgtcgcaa
     2341 acacgttcgc gccgcctgac gtcggccgca tggacgtggc cgcgatgaac gcgaacaagg
     2401 tcggcgacac gctgatcgcc gacggccgcg tccgcaaggg caagctgatc gccgacacca
     2461 ccatcccgcc cgacatcggc cagagcat
//
LOCUS       DASTZR010000252         2132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1162722, whole genome shotgun sequence.
ACCESSION   DASTZR010000252 DASTZR010000000
VERSION     DASTZR010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162722"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VFV20_05590"
     CDS             complement(<1..721)
                     /locus_tag="VFV20_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEU5287857.1"
                     /translation="MRRAVIAGGWRTPFVKAGTELADADVLELATAATAETLARTETA
                     PKTVDEIVYGNVSRPVAYHNLAREIVLALDLPTSIYAASVGMACASACVAITDAGDHI
                     ALGTADAVLAGGAESLSNVPITYSPKLARALVRASGAKTLPQRVTSFAGISLKDLSPV
                     APGIKETSTGLTMGESAEKMAAINGIAREDQDRWALRSHQNAAAGWDDGRLAAEVVPV
                     RRRGTAVARDTHIRRDTTLERL"
     gene            complement(776..1384)
                     /locus_tag="VFV20_05595"
     CDS             complement(776..1384)
                     /locus_tag="VFV20_05595"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEU5287858.1"
                     /translation="MLDALLLGALAGYAIAIPVGPIAVLILRTGMRDGLRAAMAAGAG
                     AATADLVYSSLAMLAGPALVAAIAPILVPARLVAAALLLAVAALGLRRTPPADETRRA
                     TPTHRTYATVLALTLLNPATVVYFASLAIGLPSLSSELAARVAFAGAAATASLSWQWA
                     LAGAGSALHGRMPARLGYATNVLSAVIIGGLALKIAADALVG"
     gene            complement(1401..>2132)
                     /locus_tag="VFV20_05600"
     CDS             complement(1401..>2132)
                     /locus_tag="VFV20_05600"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU5287859.1"
                     /translation="VSGLLGIGGAVVLGPLLLYLPGVMGAAAIPVKIMTGLTVVQAIS
                     GSIIGSWRHRAYGNVSTRTVVLMGPPAAVASLAGALVSRTSSDALLLVIFAAFSFLTA
                     LFVLLPPRTIQARAEHVRVNVPLALALAIGIGFFGGMVGIGAIATILAVLIYLLRVPP
                     RVAIGTSLGVGMFSAIAALIGKAATAQIDPPLALAVVVSALVASPIGARISIRTRTTV
                     LMRILAVVVILSGLRILWTALFETV"
BASE COUNT          333 a          729 c          811 g          259 t
ORIGIN      
        1 cgagccgctc gagcgtcgtg tcgcggcgga tgtgcgtgtc gcgggcgacc gccgtcccgc
       61 ggcgacgcac ggggacgacc tcggcggcga gccggccgtc atcccagccg gcggccgcgt
      121 tctggtggct gcgcagcgcc cagcggtcct ggtcctcgcg cgcgatgccg ttgatggcgg
      181 ccatcttctc agcggactcg cccatcgtga gcccggtgga agtctccttg atgcccggcg
      241 cgacggggga gaggtctttg agggagatgc cggcgaagct cgtcacgcgc tgcggcagcg
      301 tcttcgcccc tgacgcgcgc acgagcgcgc gcgcgagctt cgggctgtac gtgatgggga
      361 cgttcgaaag gctctcggcg ccgcccgcca ggacggcgtc cgccgtgccg agcgcgatgt
      421 ggtcaccggc atcggtgatc gcgacgcatg ccgacgcgca ggccatgccg acgctcgcgg
      481 cgtagatgct ggtggggaga tcgagggcga gcacgatctc gcgagcgagg ttgtgatacg
      541 ccacgggccg cgagacgttg ccgtacacga tctcgtcgac cgtcttcggt gccgtctcgg
      601 tccgggcgag cgtctcggcg gtcgcggccg tcgccagctc cagcacgtcc gcgtcggcga
      661 gctcggtccc tgccttcacg aatggggtgc gccagccgcc cgcgatcacc gcccgtcgca
      721 tcgacccatc gtaccaaacg accgtttggt caaggaacgc cgggacggcg ctcgctcagc
      781 cgaccagtgc gtccgccgcg atcttcagcg ccagcccgcc gatgatcacc gcgctcagga
      841 cgttcgtcgc gtagccgagc cgcgcgggca tccgcccgtg cagggccgat ccggcgccgg
      901 cgagcgccca ttgccacgac agcgacgcgg tggcggcggc cccggcgaag gcgacgcgtg
      961 ccgcgagctc gctcgagagc gaaggcagcc cgatggccag cgaggcgaag tacacgaccg
     1021 tggcggggtt gagcagcgtc agcgcgagga ccgtcgcgta cgtgcggtgc gtgggggtcg
     1081 cgcgtctcgt ctcgtcggcc ggcggcgtgc ggcgcagacc cagcgccgcg accgcgagca
     1141 gcagcgccgc ggcgaccaga cgcgcgggga cgaggatcgg cgcgatggcg gcgacgagcg
     1201 ccggcccggc gagcatcgcg aggctggaat agacgaggtc cgcggtcgcg gcgcccgcgc
     1261 ccgcggccat cgcggctcgc agaccgtcgc gcatgccggt gcgcaggatg agcaccgcga
     1321 tcggtccgac ggggatcgcg atcgcgtagc cggcgagggc gccgagcagg agagcgtcga
     1381 gcacgtcgga acgatagtgc tcagaccgtc tcgaagagcg cggtccagag gatgcgcagg
     1441 cccgacagga tgacgacgac ggcgaggatg cgcatgagca cggtcgtgcg cgtgcggatg
     1501 gagatgcgcg cgccgatggg tgacgcgacg agcgccgata cgaccaccgc cagcgcgagg
     1561 ggtggatcga tctgagccgt cgccgccttc ccgatgagcg ccgcgatcgc gctgaacatg
     1621 ccgacgccca gcgacgtccc gatcgcgacg cgcggcggta cccggaggag atagatgagg
     1681 accgcgagga tggtcgcgat cgcgccgatg ccgaccatgc cgccgaagaa gccgatgccg
     1741 atggcgagcg cgagggcgag cgggacgttc acgcgtacgt gttcggcccg cgcctggatc
     1801 gtacgcgggg gcaggagcac gaaaagcgcg gtgaggaacg agaacgccgc gaagatgacg
     1861 agcaggagcg cgtccgacga cgtcctcgag acgagcgcac ccgccagcga cgcgaccgcc
     1921 gccggcgggc ccatgagcac gacggtgcgc gtggagacgt tgccgtacgc gcggtggcgc
     1981 cacgacccga tgatgcttcc ggagatcgcc tgcacgaccg tgaggccggt catgatcttc
     2041 accgggatcg ccgccgcgcc catgacaccc ggcagataga ggagcagcgg tccgagcacc
     2101 accgcgcccc cgatgccgag cagtccggac ac
//
LOCUS       DASTZR010000253         2272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_62899, whole genome shotgun sequence.
ACCESSION   DASTZR010000253 DASTZR010000000
VERSION     DASTZR010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62899"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="VFV20_05605"
     CDS             complement(<1..892)
                     /locus_tag="VFV20_05605"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEU5287860.1"
                     /translation="MGSLTAGERVTGGPRLHYLDWLRVVAIASVFVFHTLRPFGDEWH
                     VNNAERSQALEDALFFFWTFGLALLFLVAGAGVRFALRRRDSSTFVRERFSRLLVPFA
                     VGTLLLAPPQAFIEAVHKGADTGRPLDFIGRWGAGVIEAFGRGISPTVFGVGFHLWFL
                     GFLFAISIVALPVCRLLLSGRGLAATTAWAERSGWRGASLVFAVPIAIVIGIAAPFSS
                     GEHDWFEVGWYLVYFVNGFVLFSDERFIAAVRRDAGVALAIALASTVALARIDMGAWA
                     EALPERGIDWSYPAMGGLFAV"
     gene            1074..1514
                     /locus_tag="VFV20_05610"
     CDS             1074..1514
                     /locus_tag="VFV20_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287861.1"
                     /translation="MSQVAYDRYVVELPPADADWRPLADPETLAETAAWLWEFAPTPL
                     VAVVGADGAAPSWLASWSPREMHWAPEGTTSGHAVVITTQADLERFLAEGAPHEHTVL
                     LWPRVSPAKTFEALGAGGAAWLGAVDAHAKISRHGEVFEISQNG"
     gene            complement(1498..1674)
                     /locus_tag="VFV20_05615"
     CDS             complement(1498..1674)
                     /locus_tag="VFV20_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287862.1"
                     /translation="MSALALTLAAPRAAIAVALAIGLPATVLNGSVPALLFSSVGAGL
                     GYVVRRSAAASRSD"
     gene            complement(1776..>2272)
                     /locus_tag="VFV20_05620"
     CDS             complement(1776..>2272)
                     /locus_tag="VFV20_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HEU5287863.1"
                     /translation="RAMPNTPAQIGMGVTAWTATSEVDREQRDRAKAILGALGEEIEV
                     DEESQVDMATALSGTGPSYVFLLMEALVDAGVHLGFSRRVAEELVLRTVEGSAAFARQ
                     SDRHLAELRNMVTSPGGTSAAALYELEKGTLRTVLSRAVYAAYLRTRELGLEAEARSK
                     KERS"
BASE COUNT          372 a          829 c          785 g          286 t
ORIGIN      
        1 cgaccgcgaa gaggccaccc atggccggat aggaccaatc gatcccgcgt tcgggtagcg
       61 cctccgccca cgcgcccatg tcgatgcgcg cgagcgcgac cgtgctcgcg agagcgatcg
      121 cgagcgcgac accggcgtcg cggcgcacgg cggcgatgaa gcgttcgtcg gagaacagca
      181 cgaatccgtt gacgaagtac acgaggtacc agccgacttc gaaccagtcg tgctcgccgc
      241 tgctgaacgg cgcggcgatc ccgatcacga tcgcgatcgg taccgcgaag accagcgacg
      301 caccgcgcca gccgcttcgt tcggcccacg cggtggtcgc cgcgagaccg cgtccgctca
      361 ggagcagcct gcaaacgggc agcgccacga tcgagatcgc gaacaggaat ccgaggaacc
      421 agagatggaa gcccacgccg aagacggtcg gtgagatgcc gcgtccaaat gcctcgatca
      481 cgccggcccc ccagcgaccg atgaagtcga ggggccgccc cgtgtcggcg cccttgtgca
      541 ccgcctcgat gaaggcctgc ggcggtgcga gcagcagcgt gccgactgcg aacggaacga
      601 ggaggcgcga gaagcgctcg cgcacgaagg tcgacgagtc gcgcctgcgg agcgcgaagc
      661 ggacgcccgc ccccgccacg aggaacagca gcgcgagtcc gaaggtccag aagaagaaca
      721 gggcgtcctc gagcgcctga ctgcgctcgg cattgttgac atgccactcg tcgccgaacg
      781 gccgcagcgt gtggaacacg aagaccgagg cgatcgcgac cacgcggagc cagtcgaggt
      841 agtgcagccg cgggccgccg gtcacgcgct ccccggcggt caacgatccc accacgtacc
      901 ctccgcgccc cttctagcac cggcgaaccg tcgtcgctgt agggcgagcc ccgacccgcg
      961 ggcgctcgta cggctgcggc cgacgcggtc cggtacgcaa gtactaggga ccccgccccc
     1021 gacgggggct gccctgggct cacgcgcggt ggtactccgg acgtgacatg caggtgagcc
     1081 aggtcgcata cgaccgctac gtcgtggagc tcccgccggc cgacgccgat tggcgtccgc
     1141 tcgccgaccc ggagaccctc gccgagacgg ccgcgtggct ctgggagttc gctccgacac
     1201 cgctcgtcgc ggtcgtcggt gccgacggcg ccgcaccgag ctggctcgcc tcgtggtcac
     1261 cgcgcgagat gcactgggca cccgagggaa cgacctcggg tcacgccgtg gtcatcacga
     1321 cgcaggcgga cctcgaacgc ttcctggccg agggcgcgcc gcatgagcac accgtgctcc
     1381 tgtggccgcg cgtctccccg gcgaagacgt tcgaggccct cggggcgggc ggcgctgcct
     1441 ggctgggcgc cgtcgacgcg cacgcgaaga tcagccgtca cggcgaggtc ttcgagatca
     1501 gtcagaacgg ctagctgccg cactccttct gacgacgtag ccgagcccag cgcccaccga
     1561 agagaacagc agcgccggca cgctcccgtt cagcacggtc gccggtagcc cgatcgcgag
     1621 cgcgacggcg atcgccgccc gtggcgcagc gagcgtcagc gcgagcgcgc tcaccgtgaa
     1681 cagcaggacc tcgatggcgg tgtcatccgt tcgccggtcg acggtgagca gccagtagcc
     1741 gacgatcagc gcggcggcga tgacggcccc gcgtctcagg accgctcctt cttcgagcgc
     1801 gcctccgcct cgagaccgag ctcgcgcgtc cgcagatagg ccgcgtacac ggcccggctg
     1861 agcaccgtgc gcagcgtgcc cttctcgagc tcgtagaggg cggccgcgct cgtcccgccg
     1921 ggcgacgtga ccatgttgcg cagctccgcg aggtgccggt cgctctggcg agcgaaggcc
     1981 gccgagcctt cgacggtgcg caggacgagc tcctccgcga cgcgccgcga gaagccgaga
     2041 tgcacgccgg cgtcgacgag ggcctccatg agcaggaaga cgtagctcgg gccggtgccg
     2101 gagagcgccg tggccatgtc gacctggctc tcctcgtcga cctcgatctc ctcgccgagc
     2161 gcgccgagga tagccttcgc ccggtcgcgc tgctcgcggt ccacctcgct cgtcgccgtc
     2221 cacgccgtga cgcccatgcc gatctgcgcc ggagtgttgg gcatcgcacg ca
//
LOCUS       DASTZR010000254         2742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_820207, whole genome shotgun sequence.
ACCESSION   DASTZR010000254 DASTZR010000000
VERSION     DASTZR010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820207"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1116)
                     /gene="ligD"
                     /locus_tag="VFV20_05625"
     CDS             complement(91..1116)
                     /gene="ligD"
                     /locus_tag="VFV20_05625"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEU5287864.1"
                     /translation="MLVGGREVNVTNPEKVFFPAVGVTKGDLVQYYADLAGPTLNHVA
                     RRPMQMKRHPNGVDGDFFYQKRVPNPHPDWLETVRIEYPSGNVADFPVVTDAAGLAWI
                     ANLGCIEMHTWHSRVPAFTKPDYMLIDLDPSEGNPWEHVREIALVVKQVLDELSLPSY
                     PKTSGVSGIHILTPIVPELPFPEVRRLAKAIAVEIAKRVPKIATTTWVVKDRTGVFVD
                     YGQNAFDRTIASAYSIRPMADARASAPLRWDEVADVDPARFTLLTMRERVAAVGDLTA
                     DIWSHAVSLVPVFKTLHLKEPVLDDRVLVRPSRVREWSEEEQKAWRERVRATGWNRGK
                     SRRPPKV"
     gene            complement(1133..2587)
                     /locus_tag="VFV20_05630"
     CDS             complement(1133..2587)
                     /locus_tag="VFV20_05630"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HEU5287865.1"
                     /translation="MPPRRAAILVLAAAAFGLVFQYLFVRERTGANVVAATALFLALT
                     WAFRDRGRHFAWRDAWLPLGALLFAALCAIRTDALLVAFDALASVALALGTVVASRGV
                     PVSDLPLGRLIATGLAACGALLSRMVMACAVAIPALGAFAPGRASRMVGYGGGVVLAV
                     PFLVVFAVLFSSADAVFQRTLADALDFVRWRELLADAPWRATLATVAMWLAGGALAWL
                     AEPPSTDRDTRPAGLVGADPVTAMLVAIDALFAFFVVVQIAYLFGGRDTVAAAGIAYS
                     AYARRGFFELVAVATLVGGLLFSVDLVVRERGRAYRTAAILLVALTAVVLASAALRMA
                     LYQQAYGWTELRLYACAATLYLALALAVLAWAVARSRMEVALQRLVLAGIVVAIATNA
                     VGPSDLIARANIERATSPALPADVSRDLDVCYLVSLGDGAVPAIMRALPSLPDGERTR
                     TLELLRADATRRPGPGGWQSWNLDRRRAEEVLPR"
BASE COUNT          438 a          973 c          991 g          340 t
ORIGIN      
        1 tgccgcggtc ttcgtcctgg acgtcgccgc gacgctctac ctcgcgatcg cgcgctggaa
       61 ctggaagggc gaggtccgcc tctcggacat ctagaccttc ggcgggcgcc ggctctttcc
      121 gcggttccac cccgtcgcgc gaacgcgctc acgccatgcc ttctgctcct cttcgctcca
      181 ctcacgaacg cggctcggcc gcacgagcac gcgatcgtcg agcaccggct ccttgagatg
      241 gagcgtcttg aacacgggga cgaggctcac cgcgtgtgac cagatgtccg ccgtgaggtc
      301 gcccaccgcg gcgacgcgct cgcgcatggt gagcagcgtg aagcgcgccg gatcgacgtc
      361 cgcgacctcg tcccagcgca gcggggcgga ggcgcgcgcg tcggccatcg ggcggatcga
      421 gtacgcggac gcgatggtgc ggtcgaaggc gttctgcccg tagtcgacga agaccccggt
      481 gcggtccttc accacccagg tggtcgtcgc gatcttcggc acacgcttcg cgatctcgac
      541 cgcgatcgcc ttcgccaggc ggcgcacctc cgggaagggc agctccggga cgatcggcgt
      601 gaggatgtgg atcccggaga cgcccgatgt cttcggatag gacggcagtg acagctcgtc
      661 gagcacctgc ttcaccacga gcgcgatctc tcgcacgtgc tcccacggat tcccctcgct
      721 cgggtcgagg tcgatgagca tgtaatcggg cttggtgaac gcggggacgc gcgagtgcca
      781 agtgtgcatc tcgatgcagc cgaggttggc gatccacgcg agtcccgccg cgtcggtgac
      841 caccggaaag tcggcaacgt tcccgctcgg gtactcgatg cgcacggtct cgagccagtc
      901 gggatgcggg ttcggcacgc gcttctggta gaagaagtcg ccgtcgacgc cattcggatg
      961 ccgcttcatc tgcatcgggc ggcgcgcgac gtgattcagc gtgggtccgg cgaggtccgc
     1021 gtagtactgg acgaggtcac ccttcgtgac gcccacggcc gggaagaaga ccttctccgg
     1081 gttcgtgacg ttgacctcgc ggccgccgac gagcatccct ggattgtggg ggctatcgcg
     1141 ggagcacttc ctcggctctg cggcgatcga ggttccagct ctgccagccg cccggccctg
     1201 gtcgccgcgt cgcgtcagct cgcaggagct ccagcgtcct ggtgcgctcg ccatcgggga
     1261 gcgagggcag cgcccgcatg atcgccggca ccgcgccgtc acccaggctc acgaggtagc
     1321 acacgtcgag gtcccgcgag acgtcggcgg gaagcgccgg cgacgtggcc cgctcgatgt
     1381 tcgcgcgcgc gatgaggtcg gatggtccga ccgcgttcgt ggcgatcgcc acgacgatgc
     1441 ccgcgagcac gagccgctgg agcgcgacct ccatgcgcga gcgagcgacc gcccacgcga
     1501 ggaccgcgag cgcgagcgcg aggtacaggg tcgcggcaca cgcgtagagc cgcagctccg
     1561 tccacccgta cgcctgctgg tacagcgcca tgcgcagcgc cgccgaggcg agcacgacgg
     1621 cggtgagcgc gacgagcagg atcgccgcgg tccggtacgc gcgaccgcgc tcgcgcacga
     1681 ccagatccac cgagaagagc agcccgccca ccagcgtcgc gactgcgacg agctcgaaga
     1741 agccgcggcg cgcgtacgcg ctgtacgcga tacccgccgc ggcgacggtg tcgcgcccgc
     1801 cgaagaggta agcgatctgg accacgacga agaacgcgaa cagcgcgtcg atggcgacga
     1861 gcatcgcggt gacggggtcc gcgccgacga gcccggcggg ccgcgtgtcg cggtccgtcg
     1921 acggcggctc cgcgagccac gcgagcgcgc cgccggccag ccacatcgcc acggtcgcca
     1981 gcgtcgcgcg ccacggcgcg tcggccagca gctcgcgcca tcgcacgaaa tccagcgcat
     2041 cggcgagcgt gcgctggaag accgcgtcgg cggaggagaa gaggacggcg aacacgacga
     2101 ggaacggcac ggccagaacg acaccgccgc cgtagcccac catccgtgac gcgcgccccg
     2161 gcgcgaacgc gccgagcgcc gggatcgcga cggcgcacgc catgaccatg cgggacagca
     2221 gcgcgccgca cgcggccagg ccggtcgcga tgagccgtcc gagcggcagg tcggacaccg
     2281 gcacgccgcg cgacgcgacg accgtgccga gcgccagcgc cactgacgcc agcgcgtcga
     2341 aggcgacgag gagcgcgtcg gtacgtatcg cgcagagcgc cgcgaagagg agcgcaccga
     2401 gcggcagcca ggcgtcgcgc cacgcgaagt ggcgcccgcg gtcgcggaac gcccacgtga
     2461 gcgcgaggaa gagcgcggtc gcggcgacga cgttcgcacc ggtccgctcc cgcacgaaga
     2521 ggtactggaa gacgagaccg aacgccgccg cggcgagcac gaggatggcg gcgcgtcggg
     2581 gcggcaccgg gccagcgtcg gacctcgtgc gcccgcggtc atcgcacgcc cgagatgtcc
     2641 cacgcgtgag acgcgccccg ggacgcggtg ggcgtagcct ctaggccgtg cggatacgcg
     2701 ataccgagcg gctctcacgc gcgatcggga ccctcggcgg cg
//
LOCUS       DASTZR010000255         5423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1189114, whole genome shotgun sequence.
ACCESSION   DASTZR010000255 DASTZR010000000
VERSION     DASTZR010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5423
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189114"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VFV20_05635"
     CDS             complement(<1..387)
                     /locus_tag="VFV20_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5287866.1"
                     /translation="MTTALRVAIVDDERLFAELLRVALSNAADVEVVGVLHDVATTVA
                     QVPTLRPAVVLADYHLPDGTGAELARSLRRIAPETSVVVLTGDPSSGTLADVARSGAV
                     GHLTKASGLEEVVRAVRAAAAGEILFA"
     gene            complement(384..1688)
                     /locus_tag="VFV20_05640"
     CDS             complement(384..1688)
                     /locus_tag="VFV20_05640"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEU5287867.1"
                     /translation="MTVTNDLKPATRVVVVEDDPASADVLQRRLQANGMTVAVGRDGG
                     EGLALVKQIVPDLVLLDVMLPDTDGYDVCLRIKSDGNTAHIPVIFLSARGEVVDKVRG
                     LSCGAADYVTKPWNAAELLARIDAVLQQARSARPPRLERPSTRPPRARPVAAIALSDP
                     ARRHRAKTLLASAFDIADEGGQGPDLVLVDAGGMVPEDLADDATIVLEIVGDGVAPDP
                     EAARLDDELPRVASLLVRERRIEHDLDAAADALITLATALESHDRSTMGYSERVAARA
                     VAMAQVLGLDDHAIRTIRLGALLRDIGNAKLPPALVHKQGELTASEREVVERHPILGE
                     EVLRGIAPLRAILPIVRSHHERLDGGGYPDHLRADALPLEVRVVTVADRFEALLVDRP
                     DRAKVSEPEAVRILQGEVTKGELDPAVVAALAAQVRSMEGVR"
     gene            complement(1685..2443)
                     /locus_tag="VFV20_05645"
     CDS             complement(1685..2443)
                     /locus_tag="VFV20_05645"
                     /inference="COORDINATES: protein motif:HMM:NF020916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HEU5287868.1"
                     /translation="MTTLSTTRVITTNEPRKEHRFLVNEAVARLALRTAGTYLPLDRE
                     DRPYQWSTTTYCDTYDFSVLRAEKAGHAVQLRLREYHRTRPNDAFSSEEIFLEMKDDS
                     PGASTKERYRVPTKEVPAYLRGERKIDAQSGPLATKIAELVTAGVRPVVVTQYNRIAY
                     SAPDGTVRITADHNLMYLALPWEANSDDAVPCKLGPVIGREPGVVIEVKWFDELPHWA
                     DELYGYIHEHMVNERPSKYVIAMRHLLGEQELTA"
     gene            complement(2443..3951)
                     /locus_tag="VFV20_05650"
     CDS             complement(2443..3951)
                     /locus_tag="VFV20_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287869.1"
                     /translation="MNERLRRILADVMPLRRGEWRLALFLYGVLTLMVAADWVGKLGA
                     DALFVKRFGVNEIPKMYVVTPIVMLAVSALIFYFVDRVRRRTMLLWYVAAVTVSSLLI
                     QWVISSGATGVIQPISYIFAHGVKETIYILFWVYAGNLYDVEQSKRLFPFFAGSVLVG
                     KILGGAVGAAIAPVVHAENFIGAQAVGFAVCFVAILLYRNLPEGEGRHARVAHRPTGL
                     RDSMRETVDGYRTVGADRLLRVFGVGVFFWYFLMQFANFLYLVGLDQSSASAPSRMGQ
                     EDVFSILYASVYTSASLVALGIQSFMTTPLVRRFGVARVLFALPVWYIASYGVASYAY
                     GGDIGWLVLPAVIALQLGERIVIPAIHRPATELVYSQISAAIRPRARAFLSGGVNAVG
                     NMAAAIALIVGIANFSTTALLAAGAGASLLYAVNAWQTRRAFGQRIAENLSSQDPAVR
                     RNAVEMLHAEGAAVPTELLRAVVEEGSDEVAHGARLALTRRGALAVAVDSAE"
     gene            complement(3948..>5423)
                     /locus_tag="VFV20_05655"
     CDS             complement(3948..>5423)
                     /locus_tag="VFV20_05655"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5287870.1"
                     /translation="RIIRAAGHEPLAATDGASGLATARAEHPDAILVDLLLPRIDGWT
                     VTKTLREDEWAKKIPIIAVSALAMQQDRARAIEAGCDDFVSKPYAPAELRAVLSRFLD
                     ERGAEPEAAARARAAHQPIERLGRVLVVDDEPANVELLVRRLESLGADTLRAANGEDA
                     LVIADRELPDLILLDIMMPGIDGYDTCRRFKVNDRTAPIPVIFVTAKDRADDLSKGFE
                     VGGVDYVTKPIEPIELAARVRSAIFTKRLQDELRARNAQLHSLERSRQELIGMLGHDI
                     RNLANSLVAFLQLVRLGQLDASRPEFGELLGLSESNVTELLRMVNALLDVYKMEEGRL
                     QADPQLIRLDALAKKSLDQLAAEARARGVELSVEAGNVSVYVDETLMVRVLTNLLANS
                     VKHTPSGGTARVTAMAGADPNWITVRVCDTGPGIPVVDAANVFDRFYQTAGGRSRGGT
                     GLGLAFCKLAVELHGGAIKVANPGEPGAVIELTMPAAVARA"
BASE COUNT          812 a         1905 c         1937 g          769 t
ORIGIN      
        1 tgcgaacagg atctcgcccg ccgcggccgc gcgcacggcc cggacgacct cctccaatcc
       61 gctggccttg gtgaggtggc cgacggcccc cgaccgcgcg acgtccgcga gggtgccgct
      121 cgacggatcg cccgtcagca ccacgaccga ggtctccggt gcgatgcgcc gcagcgaccg
      181 cgcgagctcc gcgccggtcc cgtcggggag gtggtagtcg gcgagcacga ccgccggacg
      241 caacgtgggg acctgggcga ccgtcgtggc gacgtcgtgc agcacgccca cgacctcgac
      301 gtccgccgcg ttcgagagcg cgacgcgcag gagctcggcg aagagacgct cgtcgtcgac
      361 gatggcgacg cggagcgcgg tcgtcatcgc acgccctcca tcgagcgcac ctgcgcggcc
      421 agcgcggcga cgacggcggg atcgagctcg cccttcgtga cctctccctg gaggatccgc
      481 accgcctccg gctcggagac cttcgcgcgg tccggccggt cgacgagcag cgcctcgaag
      541 cggtcggcga cggtcaccac ccgcacctcg agcggaagcg cgtcggccct caggtgatcg
      601 ggatagccac caccgtcgag gcgctcgtga tgcgagcgca cgatcggcag gatcgcgcga
      661 agcggcgcga tgccgcgcag gacctcctcg ccaaggatgg ggtggcgctc cacgacctcc
      721 cgctcgctgg cggtgagctc gccctgcttg tgcaccaggg ccggcggcag cttcgcgttg
      781 ccgatgtcgc gcagcagcgc gccgagacgg atcgtccgga tcgcgtggtc gtcgagcccg
      841 aggacctgcg ccatcgcgac ggcgcgagcg gctacgcgct ccgagtagcc catcgtcgaa
      901 cggtcgtggc tctcgagcgc ggtcgcgagg gtgatgaggg cgtcggccgc agcgtcgagg
      961 tcgtgctcga tgcgccgctc gcgcacaagg aggctcgcca cgcgcggcag ctcgtcgtcg
     1021 agccgggccg cctccgggtc cggggccacg ccgtcgccga cgatctcgag gacgatggtc
     1081 gcgtcgtccg cgagatcctc cggcaccatc ccacccgcgt ccacgagcac caggtccggc
     1141 ccctgcccac cctcgtccgc gatgtcgaac gccgacgcga gcagcgtctt cgcgcgatgc
     1201 cggcgcgccg gatccgacag cgcgatcgcg gccacggggc gcgcgcgggg gggccgcgtg
     1261 gacggacgct cgaggcgcgg cggccgcgcg ctgcgcgcct gctggagcac cgcatcgatg
     1321 cgcgccagga gctcggcggc gttccacggc ttggtcacgt agtccgcggc gccgcacgag
     1381 agaccgcgca ccttgtccac cacctcgccg cgcgccgaga ggaagatcac cgggatgtgc
     1441 gcggtgttgc cgtcgctctt gatgcgcagg cacacgtcgt agccgtcggt gtcgggcagc
     1501 atgacgtcca ggagcacgag gtccggcacg atctgcttga ccagcgcgag gccctcgccg
     1561 ccatcacggc cgaccgccac ggtcatgccg ttcgcctgca ggcggcgctg gagcacgtcg
     1621 gccgacgccg ggtcgtcctc caccacgacg accctcgtgg cgggcttgag atcgttcgtg
     1681 accgtcatgc cgtgagctcc tgctcgccga ggaggtggcg catggcgatg acgtacttcg
     1741 acggacgctc gttgaccatg tgctcgtgga tgtagccgta cagctcgtcg gcccaatgcg
     1801 ggagctcgtc gaaccacttc acctcgatga cgacacccgg ctcgcgcccg atgaccgggc
     1861 cgagcttgca cgggacggcg tcgtcggagt tcgcctccca cggaagcgcc aggtacatga
     1921 ggttgtggtc cgcggtgatc cgcaccgtgc cgtcgggcgc cgaatacgcg atgcggttgt
     1981 actgcgtcac cacgaccggc cgcacgcccg ccgtcaccag ctcggcgatc ttcgtcgcga
     2041 gcggtcccga ctgcgcgtcg atcttccgct cgccgcgcag gtaggccggg acctccttcg
     2101 tgggcacgcg gtagcgctcc ttcgtcgagg cgcccggcga gtcgtccttc atctcgagga
     2161 agatctcctc gctgctgaac gcgtcgttcg gccgcgtccg gtggtattcg cgaaggcgca
     2221 gctgcacggc gtgcccggcc ttctcggcgc gcagcacgct gaagtcgtag gtgtcgcagt
     2281 aggtcgtggt cgaccactga tagggacggt cctcgcggtc gagcggcagg taggtcccgg
     2341 cggtgcggag cgcgaggcgc gcgacggcct cgttcacgag gaagcgatgc tccttgcgag
     2401 gctcgttcgt ggtgatgaca cgggtggtcg agagcgtcgt catcattcgg ccgagtccac
     2461 ggcgacggcg agcgcgccgc ggcgtgtcag cgcgaggcgc gccccgtgcg ccacctcgtc
     2521 gctgccctct tcgacgacgg cccggagcag ctcggtcggc accgccgcgc cctccgcgtg
     2581 cagcatctcc accgcgttcc ggcgcaccgc cggatcctgc gaggagaggt tctcggcgat
     2641 gcgctggccg aacgcgcggc gggtctgcca ggcgttcacg gcgtacagga gcgaggcgcc
     2701 cgcgccggcg gcgaggagcg ccgtggtgct gaagttggcg atgccgacga tgagcgcgat
     2761 cgccgcggcc atgttgccga ccgcgttcac gccgcccgac aggaacgcac gggcccgcgg
     2821 acggatcgcc gccgagatct ggctgtagac cagctcggtc gccggccgat ggatggccgg
     2881 gatcacgatg cgctccccga gctgtagcgc gatgaccgcg gggaggacga gccagccgat
     2941 gtcaccgccg taggcgtacg aggcgacgcc atacgacgcg atgtaccaga ccggcagcgc
     3001 gaagagcacg cgcgccacgc cgaagcggcg gaccagcggc gtcgtcatga acgactggat
     3061 gccgagcgcg accagggacg cgctcgtgta gaccgaggcg tagaggatgc tgaagacgtc
     3121 ctcctgcccc atccgcgacg gcgccgacgc cgaggactgg tccaggccga cgagatacag
     3181 gaagttcgcg aactgcatga ggaagtacca gaagaagacg cccacgccga agacgcggag
     3241 cagccggtcg gcgccgacgg tgcgatagcc gtctaccgtc tcgcgcatgc tgtcgcgcag
     3301 tccggtcgga cggtgcgcga cccgggcgtg acggccctcg ccctccggca ggttccggta
     3361 cagcaggatg gcgacgaagc acacggcgaa cccgaccgcc tgcgcaccga tgaagttctc
     3421 cgcgtgcacg accggcgcga tggccgctcc gacggcgccg cccaggatct tgccgacgag
     3481 cacggacccg gcgaagaacg ggaagagccg cttggactgc tcgacgtcat agaggttccc
     3541 cgcgtacacc cagaacagga tgtagatggt ctccttcacg ccgtgggcga agatgtacga
     3601 gatcggttgg atcacgccgg tcgctccgct cgagatgacc cactggatga gcagcgacga
     3661 gacggtcacc gccgcgacgt accagagcag catcgtccgc cggcgcacgc gatccacgaa
     3721 gtagaagatg agcgccgaca ccgcgagcat gacgatgggt gtgaccacgt acatcttcgg
     3781 gatctcgttc acgccgaacc gcttgacgaa gagcgcatcg gcgccgagct tgcccaccca
     3841 gtcggcggcg accatgagcg tcagcacgcc gtagaggaac agcgcgagtc tccactcgcc
     3901 ccggcggagc ggcatcacgt cggcgaggat ccgccgcagt cgctcgttca cgcgcgcgcc
     3961 accgccgcgg gcatcgtcag ctcgatgacg gctccgggct cgccggggtt cgcgaccttg
     4021 atggcgccgc cgtgcagctc gaccgcgagc ttgcagaagg cgagaccgag acctgtgccg
     4081 ccgcgactgc ggccgccggc ggtctggtag aagcgatcga agacgttcgc ggcgtccacc
     4141 accgggatgc ccggtccggt gtcgcagacg cggaccgtga tccagttcgg gtcggcgccg
     4201 gccatcgccg tgacgcgggc ggtgccgccg ctcggggtgt gcttcaccga gttcgccaga
     4261 aggttcgtga gcacacgcac catcagcgtt tcgtccacgt acacggacac gttgcccgct
     4321 tcgacggaca gctccacgcc gcgcgcgcgg gcctccgccg cgagctggtc gaggctcttc
     4381 ttggcgagcg cgtcgaggcg gatgagctgc gggtcggcct ggaggcgtcc ctcctccatc
     4441 ttgtagacgt cgagcagggc gttcaccatc cgcaggagct cggtgacgtt cgactcggag
     4501 aggccgagca gctcgccgaa ctccggccgc gacgcgtcga gctgcccgag acggacgagc
     4561 tgcaggaacg cgacgaggct gttcgcgagg ttgcggatgt cgtggccgag catcccgatg
     4621 agctcctggc gtgaacgctc cagggagtgc agctgcgcgt tgcgtgcgcg cagctcatcc
     4681 tgcaggcgct tggtgaagat cgcgctgcgc acgcgcgcgg cgagctcgat cggctcgatc
     4741 ggcttggtca catagtccac gccgccgacc tcgaaaccct tggagagatc gtcggcacgg
     4801 tccttcgcgg tcacgaagat caccgggatc ggcgcggtgc ggtcgttcac cttgaaacgg
     4861 cggcacgtgt cgtacccgtc gatgccgggc atcatgatgt cgagcaggat caggtcgggg
     4921 agctcgcggt ccgcgatgac cagggcgtcc tcgccgttcg ccgctcgcag cgtgtcggca
     4981 ccgagcgact cgaggcggcg cacgaggagc tcgacgttcg ccggctcgtc gtccacgacg
     5041 aggacgcgtc cgagccgctc gatcggctga tgcgccgcgc gggcacgcgc ggccgcctcc
     5101 ggctccgcgc cccgctcgtc gaggaagcgc gacagcaccg cgcggagctc cgccggcgcg
     5161 tatggcttcg agacgaagtc gtcgcagccc gcttcgatcg cgcgcgcgcg atcctgctgc
     5221 atcgcgagcg ccgacaccgc gatgatcggg atcttcttcg cccactcgtc ctcgcggagc
     5281 gtcttggtca cggtccagcc gtcgatgcgc ggcagcagca ggtccaccag gatcgcgtcc
     5341 ggatgctcgg cgcgcgccgt ggcgaggccc gacgccccgt cggtggcggc gagcggctcg
     5401 tggcctgcgg cgcggatgat gcg
//
LOCUS       DASTZR010000256         6235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_902008, whole genome shotgun sequence.
ACCESSION   DASTZR010000256 DASTZR010000000
VERSION     DASTZR010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6235
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902008"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..1588
                     /locus_tag="VFV20_05660"
     CDS             86..1588
                     /locus_tag="VFV20_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5287871.1"
                     /translation="MATTHLRNYIDGRWVDSVSGQAFEDLDPATGEVIATATKSSTAD
                     VDKAVDAARRAAGAWRLYPAPKRGEILYRAGEIMLRRKDDLAREMTREMGKVLAEARG
                     DVQEGIDMTYYMAGEGRRQFGDVVPAELPNKWAMSMRHPHGVVAAITPWNFPFAIPTW
                     KLMPALILGNTVVFKPASYTPMLAVRLVEVLEEAGVPKGVVNLVLGAGGEIGDHLVTH
                     PGVDLVSFTGSSDAGAHLSEIAGRLLKRVSCELGGKNAIVVLDDADVELSIEGIVWSA
                     FGTSGQRCTAASRVIAHKSVANDVIDKLVGRAKKMKMGHGLDAATDLGPVVSRQAQEK
                     VASYMPIAEKEGATIAAGGRVPTEGALAKGFFHEPTVLVDVKPNMRVAQEEIFGPVTA
                     VIEVGSIDEAIKVLNSTKYGLSCSVYTRNVNNAFRFMRDAETGLVYVNAGTIGAEIQL
                     PFGGMKSTGNGHREAGRAALDVYGDWKSLYVDFSGKLQRAQMDAETNPAP"
     gene            complement(1585..2691)
                     /locus_tag="VFV20_05665"
     CDS             complement(1585..2691)
                     /locus_tag="VFV20_05665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5287872.1"
                     /translation="MGQPPWGRGPWTGGARPPWWPEGEPFPPAEWRQRRHRMRPRWAL
                     FMFLAFLVFALFLAFLLYTLVQGITMVFGSPPPAIVMVVGALFLFFTLTAGMRSARRL
                     AGPIGDLIDAVERIGRGDYSVRVRVRGPRQVRAFANAFNTMSARLERSEAERRRLLAD
                     VTHELRTPLTVVQGNIEALIDGVRPADQAHLEALLEETRVLSRLIDDLRTVSLAEAGA
                     LALHPEPTDVGALVRDAVSSFDAAAATKGIRLGVSQDDAGSASIDPLRVREILTNLIA
                     NALRYTPPEGSIDVRATGDAGTVTVTVTDTGPGIAADVLPHVFDRFTRSPESPGAGLG
                     LAIAKSLVTAHGGTIEAQSPPGQGTVIRFTLPRG"
     gene            complement(2691..3374)
                     /locus_tag="VFV20_05670"
     CDS             complement(2691..3374)
                     /locus_tag="VFV20_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5287873.1"
                     /translation="MKTVLVVDDEPKIATLARDYLEHAGFRVTSASDGKEALAAFRTA
                     PVDLIVLDLGLPLVDGLDVARSVRKTSNVPIVMLTGRGDESDRVAGLELGADDYVTKP
                     FSPKELVARVRAVLRRAELASAPKDVITAGGVTLDIPRRSVLVGDRPIDLTPTEFELL
                     ATLARAPGRVFTRAQLLDAVHGVAFESYERAIDAHVKNIRRKLERDPRAPRYLLTVHG
                     VGYRFAEER"
     gene            complement(3385..3735)
                     /locus_tag="VFV20_05675"
     CDS             complement(3385..3735)
                     /locus_tag="VFV20_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287874.1"
                     /translation="MNTARTFLALFAAIAIGLIGYQIGVTQNVAAQIPAGAAAPAYYW
                     YGPHMFGFGFLGLLVPLFFLFLFFGLMRAAFGGGRGWSGHGYGHEARRARFEELHREL
                     HGEKPTSGGPSTTT"
     gene            complement(3808..4353)
                     /locus_tag="VFV20_05680"
     CDS             complement(3808..4353)
                     /locus_tag="VFV20_05680"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEU5287875.1"
                     /translation="MTRAAVLAPGYGGGATQPILRSLAKALAAYDIDALPIAFRTRGK
                     RPSREYATELEDLRVARDELRAAHRLVALVGRSFGGRMCALLAASEPPDALAVVGHPI
                     APPDRPRPRDEQALLDMRCRTLVVQGERDELGPVAVLERIAAQNPLIDLVVIPGAGHD
                     LGAQERVAIEHVARWLDAAMR"
     gene            complement(4350..>6235)
                     /gene="ligD"
                     /locus_tag="VFV20_05685"
     CDS             complement(4350..>6235)
                     /gene="ligD"
                     /locus_tag="VFV20_05685"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEU5287876.1"
                     /translation="FAFEAKWDGVRTLAFVDGGVVRLQTRNLLDCTAQYPEAQQAAEA
                     LTGAYQAILDGEIVALDERGVPSFQRLQPRMHVRDEASVKKLRRTTPVIYEVFDVLWL
                     DGRDVTKRPLRERQKLLDEVLTPMGSIRRSEQFVGSGVALFDAVKEQGLEGIVAKRLD
                     SPYATARSAAWVKIKAFRSMECVVGGWTAGQGGRASTLGSLLLGVYRDGKLMPVGHVG
                     TGFDERTLKELLAMLRERESPRCPFEVEPRVNQPARWCLPELVCEVEYAELTREGTLR
                     HPTYRGLRADVSPQECTGEERREPTKVALRAAALAASASDGAPLAKQGARVPATLTID
                     GHTIRLTNLEKVLFPEDGYTKADLIRYYTEVSPFLVPMIRDHPLTLKLFPDGIAAAHF
                     YQKDKPDFTPRWIHTWIDRQAEREGGIDYVLGNDLATLVWLANYTAIEIHPWLARVDD
                     PDHPDFAIIDLDPSKGATWDDVKETAALVHEVLDDLGIVGFPKTTGSRGIHILVPIAR
                     RYTFDESRGFVFDVGRLARERKPKLVTLETVKDRRRGVYIDYLQNVRGKTTAGPYSVR
                     PIRRAPVSAPLQWDEIGALGRPDAFTIANMAERLAATGDLLAPSLQLAQTLPRPSKAS
                     AKR"
BASE COUNT          941 a         2171 c         2223 g          900 t
ORIGIN      
        1 catcttcctc ggcatcgtct tcgtcggggg tctgacgatc tggctcttct atggcgcgca
       61 gttctagcgc gtagggggac acaccatggc caccacacac cttcgcaact acatcgacgg
      121 ccgctgggtc gattcggtct cgggacaggc cttcgaggat ctcgatccgg cgacgggcga
      181 ggtcatcgcc accgcgacga agtcgagcac cgcggacgtc gacaaggcag tggatgccgc
      241 gcgccgcgcc gccggcgcgt ggcgcctgta tccggcaccg aagcgtggcg agatcctcta
      301 ccgcgccggc gagatcatgc tccggcgcaa ggacgatctc gcgcgcgaga tgacacgcga
      361 gatgggcaag gtcctcgccg aggcgcgcgg cgacgtccaa gagggcatcg acatgaccta
      421 ctacatggcc ggcgagggac gccgccagtt cggtgacgtc gtcccggccg agctgccgaa
      481 caagtgggcc atgagcatgc ggcacccgca cggcgtcgtc gccgcgatca cgccgtggaa
      541 cttcccgttc gcgatcccga cgtggaagct catgcccgcg ctcatcctcg gcaacaccgt
      601 ggtgttcaag ccggcgtcgt acacgccgat gctcgcggtg cgcctggtgg aggtcctcga
      661 ggaggccggc gtcccgaagg gcgtggtcaa tctcgtcctc ggcgcgggcg gcgagatcgg
      721 cgaccacctc gtcacgcatc ccggcgtcga cctcgtctcc ttcaccggct cgagcgacgc
      781 gggcgcgcac ctcagcgaga tcgcgggccg cctgctcaag cgggtctcgt gcgagctcgg
      841 cgggaagaac gccatcgtcg tgctcgacga cgccgacgtc gagctgtcga tcgagggcat
      901 cgtgtggtcg gcattcggca cgtccggcca gcggtgcacc gcggcgtcgc gcgtcatcgc
      961 gcacaagagc gtcgcgaacg acgtcatcga caagctcgtc ggccgcgcga agaagatgaa
     1021 gatgggccac ggcctcgacg ccgccaccga cctcggtccg gtcgtctcgc gtcaggcgca
     1081 ggagaaggtc gcgagctaca tgcccatcgc cgagaaggag ggcgcgacga tcgccgcggg
     1141 cggccgcgtg cccaccgagg gtgccctggc gaagggcttc ttccacgagc ccaccgtcct
     1201 cgtagacgtg aagccgaaca tgcgcgtcgc ccaggaggag atcttcggtc cggtcacggc
     1261 ggtcatcgag gtcggctcca tcgacgaggc gatcaaggtg ctgaactcga cgaagtacgg
     1321 cctctcgtgc tccgtgtaca cgcggaacgt gaacaacgcg ttccggttca tgcgcgatgc
     1381 cgagaccggc ctcgtgtacg tgaatgccgg gacgatcggt gcggagatcc agctgccctt
     1441 cggcggcatg aagtcgacgg gcaacggcca tcgcgaggcg ggacgcgccg cgctcgacgt
     1501 gtacggcgac tggaagtcgc tctacgtcga cttctccggc aagctgcagc gcgcgcagat
     1561 ggacgcggag acgaacccgg cgccctaacc gcgcggcagc gtgaaccgga tcacggtgcc
     1621 ctgacccggc gggctctgcg cctcgatcgt gccgccgtgc gcggtgacga ggctcttggc
     1681 gatcgcgagg ccgagcccgg cgccgggcga ttcgggcgag cgcgtgaagc ggtcgaagac
     1741 gtgcggcagc acgtcggcgg cgatgcccgg gcccgtatcg gtcaccgtca ccgtcacggt
     1801 gccggcgtcg cccgtggcgc gcacgtcgat gctgccctcc ggcggcgtgt agcgcagcgc
     1861 gttggcgatc aggttcgtga ggatctcgcg cacccggagc ggatcgatcg acgccgagcc
     1921 ggcatcgtcc tgggacacgc cgagacggat gcccttcgtc gccgccgcgg cgtcgaacga
     1981 ggagacggcg tcgcgcacga gcgcacccac gtcggtcggc tcgggatgca gggcgagcgc
     2041 gcccgcctcc gccagtgaga cggtccgcag atcgtcgatg agacgggaca gcacccgcgt
     2101 ctcctccagc agcgcctcca gatgcgcctg gtcggcggga cggacgccgt cgatgagcgc
     2161 ctcgatgttg ccctgcacca cggtgagcgg cgtgcgcagc tcatgcgtga cgtccgcgag
     2221 caggcgtcgc cgctcggcct cgctgcgctc gagccgggcg ctcatcgtgt tgaacgcgtt
     2281 cgcgaacgcc cgcacctgac gtgggccgcg cacccgcacg cgcacgctgt agtcgccccg
     2341 cccgatccgc tcgaccgcgt cgatcaggtc gccgatcggg cccgcgaggc gccgcgcgct
     2401 ccgcatcccg gcggtgagcg tgaagaacag gaacagcgca ccgacgacca tgacgatggc
     2461 cggcggcgga ctgccgaaga ccatggtgat cccctgcacg agcgtgtaga ggaggaaggc
     2521 gaggaagagc gcgaacacca ggaacgcgag gaacatgaac agcgcccacc gcggtcgcat
     2581 gcggtgacgc cgctgacgcc actcggccgg cgggaacggc tcgccctcgg gccaccacgg
     2641 cggacgcgcg ccgccggtcc acggtccgcg tccccaggga ggctgaccca tcagcgttcc
     2701 tcggcgaagc ggtagcccac gccgtggacc gtgaggaggt agcgcggcgc gcgcggatcg
     2761 cgctcgagct tgcgccggat gttcttcacg tgcgcgtcga tcgcgcgctc gtaggactcg
     2821 aacgcgacgc cgtgcacggc atccaggagc tgcgcgcgtg tgaacaccct gcccggcgcc
     2881 cgcgcgagcg tcgcaaggag ctcgaactcc gtcggcgtga ggtcgatggg ccggtcgccc
     2941 acgaggaccg accggcgcgg gatgtcgagc gtcacgccgc cggccgtgat cacgtccttc
     3001 ggcgctgagg cgagctcggc gcgccgcagg accgcccgca cgcgcgcgac cagctccttc
     3061 ggactgaacg gcttggtcac gtagtcgtcc gccccgagct ccaggccggc gacgcggtcg
     3121 gattcgtcgc cgcggccggt gagcatgacg atcgggacgt tcgatgtctt ccggacgctg
     3181 cgcgccacgt cgagcccgtc gacgagcggc aacccgaggt cgagcacgat gaggtcgacc
     3241 ggggcggtcc ggaacgcggc gagcgcctcc tttccgtcgg aggcgctcgt cacgcggaag
     3301 ccggcgtgct ccagatagtc ccgtgcgagc gtggcgatct tcggctcgtc gtctaccacg
     3361 agcacggtct tcatgggccg gatgctaggt cgtcgtcgac ggtccgcccg acgtgggctt
     3421 ctcgccgtgc agctcgcggt gcagctcctc gaatcgcgcg cgccgcgcct cgtggccgta
     3481 cccgtgtccg gaccagcccc ggccgcctcc gaatgcggca cgcattaggc cgaagaagag
     3541 gaagaggaag aacagcggga cgagaaggcc gaggaagccg aagccgaaca tgtgcgggcc
     3601 gtaccagtaa taggcgggcg cggccgcgcc ggccgggatc tgcgccgcga cattctgggt
     3661 cacgccgatc tgatagccga tgaggccgat ggcgatcgcg gcgaagagcg cgaggaacgt
     3721 tcgtgcggtg ttcatgtcac atctcctttc gatgccgcac cagcgtcgcg cgcggctgtg
     3781 aaggaaatgt gaaggcgctg gaacggatca gcgcatcgcg gcgtcgagcc agcgcgcgac
     3841 gtgctcgatc gcgacgcgtt cctgcgcgcc gaggtcgtga ccggcacccg ggatcaccac
     3901 caggtcgatg agcgggttct gcgcggcgat acgttcgagc acggcgaccg gcccgagctc
     3961 gtcccgctcg ccctgcacca cgagcgtgcg gcagcgcatg tcgaggagcg cctgctcgtc
     4021 tcgcggccgc ggccggtccg gcggtgcgat cggatgcccg acgaccgcga gcgcgtccgg
     4081 cggctcgctc gccgcgagga gcgcgcacat cctgccgccg aagctgcggc cgacgagcgc
     4141 gacgaggcga tgcgcggcgc gcagctcgtc acgcgccacg cggaggtcct cgagctcggt
     4201 cgcgtactcg cggctcggcc gcttcccgcg cgtgcggaac gcgatgggca gcgcgtcgat
     4261 gtcgtacgcg gccagcgcct tcgcgagcga gcgcaggatc ggttgcgtcg cgccgccacc
     4321 gtaccccggg gccaggacgg cggcgcgcgt catcgcttcg cgctcgcctt cgacggcctc
     4381 ggcagcgtct gggcaagctg gagcgacggc gcgaggaggt cccccgtcgc ggcgagacgc
     4441 tcggccatgt tcgcgatggt gaacgcgtcg ggtcggccga gcgcgccgat ctcgtcccac
     4501 tggagcggtg ccgacacggg cgcgcggcgg atgggacgca cgctgtacgg accggcggtg
     4561 gtcttgccgc gcacgttctg caggtagtcg atgtacacgc cgcgccggcg gtccttcacc
     4621 gtctcgagcg tcacgagctt cggcttccgc tcgcgcgcga gcctcccgac gtcgaagacg
     4681 aagccgcggg actcgtcgaa cgtgtacctc cgcgcgatgg gcacgaggat gtggatgccg
     4741 cgcgaccccg tcgtctttgg gaagccgacg atgccgagat cgtcgagcac ctcgtggacc
     4801 agggccgcgg tctccttcac gtcgtcccag gtcgcgccct tcgagggatc gagatcgatg
     4861 atcgcgaagt cggggtgatc cgggtcgtcc acacgcgcga gccagggatg gatctcgatg
     4921 gcggtgtagt tggcgagcca caccagcgtc gcgaggtcgt tccccaggac atagtcgatg
     4981 ccgccctcgc gctccgcctg gcggtcgatc caggtgtgga tccagcgcgg cgtgaagtcg
     5041 ggcttgtcct tctgatagaa gtgcgccgca gcgatgccgt cggggaacag cttcagggtg
     5101 agcggatggt cgcggatcat gggaacgagg aacggcgaga cctcggtgta gtaacggatg
     5161 aggtccgcct tcgtgtaccc gtcctcggga aagaggacct tctccaggtt ggtgagacgg
     5221 atggtgtgcc cgtcgatcgt cagggtcgcg gggacgcgcg cgccctgctt cgcgagcggt
     5281 gcgccgtcgc tcgcactcgc cgcgagcgcg gcggcgcgca gtgcgacctt ggtcggctcg
     5341 cgccgctcct cgccggtgca ctcctgcggt gagacgtcgg cgcgcagacc gcgatacgtc
     5401 ggatgtcgca gcgtcccctc gcgcgtgagc tccgcgtact ccacctcgca cacgagctcg
     5461 ggaaggcacc agcgcgcggg ctggttcacc cgcggctcga cctcgaatgg gcaccgcggc
     5521 gactcgcgct cgcgcagcat cgcgagcagc tccttgaggg tgcgctcgtc gaagcccgtg
     5581 ccgacgtggc cgacgggcat gagcttcccg tcgcggtaca cgccgagcag cagtgacccg
     5641 agggtggacg cacggccgcc ctgtcccgcc gtccacccgc cgacgacgca ctccatcgag
     5701 cggaacgcct tgatcttcac ccacgcggcg gagcgcgccg tcgcgtacgg cgagtccagg
     5761 cgcttcgcga cgatcccctc gagcccctgc tccttcaccg cgtcgaagag cgccacgccg
     5821 ctgccgacga actgctccga cctgcgtatg gagcccatcg gcgtgaggac ctcgtcgagc
     5881 agcttctggc gttcgcgcag cggccgcttc gtgacatcgc gcccgtcgag ccacaggacg
     5941 tcgaacacct cgtagatcac cggcgtcgtg cggcgcagct tcttcacgct ggcctcgtcg
     6001 cgcacgtgca tgcgcggctg caggcgctgg aacgacggca cgcccctctc gtcgagcgcg
     6061 acgatctcgc cgtcgaggat cgcctggtac gcgccggtca gcgcctcggc ggcctgctgc
     6121 gcctccggat actgcgcggt gcagtcgagc aggttgcgcg tctgcagccg cacgacgccg
     6181 ccgtcgacga acgcgagcgt gcggacgccg tcccacttcg cctcgaacgc gaact
//
LOCUS       DASTZR010000257         2058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_484348, whole genome shotgun sequence.
ACCESSION   DASTZR010000257 DASTZR010000000
VERSION     DASTZR010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484348"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VFV20_05690"
     CDS             <1..319
                     /locus_tag="VFV20_05690"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU5287877.1"
                     /translation="AMVPVLAVTAVGAGDRLLWVFIAMLVLQQIILNVVGPRIMSRSI
                     GIHPLFVFFALLLGARVAGFWGVVLAMPVAGILNTFAHYFWDVTIGRPGAPDAAEPLV
                     EAS"
     gene            324..1124
                     /gene="surE"
                     /locus_tag="VFV20_05695"
     CDS             324..1124
                     /gene="surE"
                     /locus_tag="VFV20_05695"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEU5287878.1"
                     /translation="MSGHPHILVTNDDGIDADALPPLAEALGAIGDVDVIVPERNWSG
                     ASHSITLFRPLRVRPTKLRSGHPAFMTDGSPTDCVRLAALGFLRHRPDLVVSGINRGA
                     NLGDDITYSGTVAAAMEGLLSNIPSIAISIAAFGGDTDYGPAASFAALLAANIIGKGF
                     TPDSLLNVNVPALPRDQIAGVEVTRLGKRNYRDQLIERLDPYGNPYYWVGGPAVTDDV
                     VDGTDVAAVRAGKISVTPITLDLTHHALIEELTTWDWGWTAPLEVSAD"
     gene            complement(1121..>2058)
                     /locus_tag="VFV20_05700"
     CDS             complement(1121..>2058)
                     /locus_tag="VFV20_05700"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5287879.1"
                     /translation="LTTLFTWRGMFALQVPLALITIVLARRFLTEQPRRTRALDLPGL
                     AAGTVMLLGINVALLEAHSWGWMSLPIVASWIVAAAAFILFIVRERRAPAPAVDLRVF
                     RSRSFVAHSLAGAAMWWALVSLSIQLALYLQNGRGLDPTQAAVVLTPWPLLGFFVVPR
                     VGLVVSRIGHRATLMTGLVAATSSAVALVSLPPDVPLALIAVLELPLGVAMSFVLVTS
                     ASGAVAEFPPEDAGVAAAIFNSVRQVGTSLGVAIPAAVYDVVTGGSLQGEIVIDGTRW
                     AMLVTAIAFAATTVVVGVLVRRSVPSVSTAGVSVS"
BASE COUNT          345 a          725 c          716 g          272 t
ORIGIN      
        1 cgcgatggtc cccgtgctcg cggtcaccgc ggtcggcgcg ggcgacagac tgctctgggt
       61 cttcatcgcg atgctcgtgc tgcaacagat catcctcaac gtcgtcggcc cgcggatcat
      121 gtcgcggagc atcggcatcc acccgctctt cgtgttcttc gcgctcctcc tcggcgcgcg
      181 ggtcgcgggg ttctggggcg tggtcctcgc gatgcccgtg gcggggatcc tgaacacgtt
      241 cgcgcactac ttctgggacg tcaccatcgg gcgtcccggg gcgccggacg cggcggagcc
      301 gctcgttgaa gcaagctagg gccgtgagcg gccatcccca catcctcgtc acgaacgacg
      361 acggcatcga cgccgacgcg ctgccgccgc tcgcggaggc gctcggcgcc atcggcgacg
      421 tcgacgtgat cgtgccggag cgcaactgga gcggcgcgag ccacagcatc acgctcttcc
      481 gaccgctgcg cgtacgcccg acgaagctgc ggagcgggca tccggccttc atgacggacg
      541 gctcgcccac ggactgcgtc cgcctcgccg cgctcggctt cctgcgccac cggcccgatc
      601 tcgtcgtgag cggcatcaac cgcggcgcga acctgggcga cgacatcacg tactccggca
      661 cggtcgccgc ggccatggag ggcctgctct ccaacatccc gtcgatcgcg atctcgatcg
      721 cggcgttcgg cggcgacacc gactacggac ccgcggcgtc tttcgcggcc ctgctcgccg
      781 cgaacatcat cgggaagggc ttcacgccgg attccctgct caacgtgaac gtgccggcgc
      841 tcccgcgcga ccagatcgcc ggcgtggagg tcacgcggct cggcaagcgc aactaccgcg
      901 accagctcat cgagcgcctg gatccgtacg ggaacccgta ttactgggtc ggcggacccg
      961 ccgtgaccga cgacgtggtc gacggcaccg acgtcgccgc cgtgcgtgcc ggcaagatca
     1021 gcgtcacgcc gatcaccctc gacctgacgc accacgcgct catcgaggag ctcaccacgt
     1081 gggactgggg ctggacggcg ccgctcgagg tgagcgcgga ctaggagacc gagacccctg
     1141 ccgtcgagac cgacgggacg ctgcgtcgca cgagtactcc gacgaccacg gtcgtggccg
     1201 cgaacgcgat cgcggtgacg agcatcgccc agcgtgtgcc gtcgatcacg atctctcctt
     1261 ggagtgatcc gccggtgacg acgtcgtaaa cggccgccgg gatcgcgaca ccgagagacg
     1321 taccgacctg acgcacgctg ttgaagatcg cggccgccac gccggcgtcc tccggcggga
     1381 actcggcgac ggccccggac gcgctcgtca cgagcacgaa gctcatcgcg acaccgagcg
     1441 gcagctcgag caccgcgatc agagcgagcg gaacgtccgg gggaaggctc accagcgcga
     1501 ccgcgctgga cgtggcagcg acgagcccgg tcatgagcgt cgcgcggtga ccgatgcgcg
     1561 agacgacgag accgacccgc ggaacgacga agaatcccag tagcggccag ggcgtaagga
     1621 cgaccgcggc ctgcgtcgga tccaagccgc gtccgttctg gaggtagagc gcgagctgga
     1681 tgctgagcga gacgagcgcc caccacatgg ccgcgccggc caggctgtgc gcgacgaacg
     1741 agcggctccg gaacacgcgc agatcgaccg ccggtgccgg cgctcgccgc tcccggacga
     1801 tgaacagaat gaacgcggcg gcggccacga tccacgatgc gacgatcggg agagacatcc
     1861 agccccacga gtgcgcctcg aggagcgcga cgttgatgcc gaggagcatg accgtgcctg
     1921 cggcaaggcc ggggagatcg agggcacggg tgcgtcgcgg ctgctccgtg aggaagcggc
     1981 gtgcgagcac gatcgtgatg agcgcgagcg gcacctgcag cgcgaacatg ccgcgccagg
     2041 tgaagagcgt cgtgagca
//
LOCUS       DASTZR010000258         2391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1137054, whole genome shotgun sequence.
ACCESSION   DASTZR010000258 DASTZR010000000
VERSION     DASTZR010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137054"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VFV20_05705"
     CDS             complement(<1..268)
                     /locus_tag="VFV20_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287880.1"
                     /translation="MRGALTKYLEPLPVPGAGIVVATSSRLRAGRTNYAFTQIPISRR
                     LHPELPPTPLWAYDDGSGLAGQAGSFGMAVVARTGAPIQATFTNA"
     gene            complement(636..872)
                     /locus_tag="VFV20_05710"
     CDS             complement(636..872)
                     /locus_tag="VFV20_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287881.1"
                     /translation="MPFKSKAQRRKFAHLLVEGKIKDETFEEWNRSTGRKDLPERVRP
                     KKAATKSKRKPAKTKTTTKKKTKPKGTAKTSRRR"
     gene            complement(874..1320)
                     /locus_tag="VFV20_05715"
     CDS             complement(874..1320)
                     /locus_tag="VFV20_05715"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5287882.1"
                     /translation="MTTARGYVAGNDRERARLVALVARCTDADLAHPMPGGWTVAGVL
                     AHAAFWDQRILVLVEQWEKGIAPREELPADVTWINDSVKPLLLALPPRKAAEIAVAIA
                     EAVDARVAALSADIIAKNEATPLLNWSRADHRGEHLDEIEHALGLR"
     gene            1429..1920
                     /locus_tag="VFV20_05720"
     CDS             1429..1920
                     /locus_tag="VFV20_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287883.1"
                     /translation="MAHAAAVQPRRDVLLHRDALTVAVAATLFAGGVMFAALWGTAQL
                     LDTSDIGLGSALSEAAFVIERDANALGADRAADATTLRTLAARLREDARFLGEHPEWN
                     TNASAALLVSKVDQLDADANALAARGAALGDASLVAAADHIRQAARMVSGAAERARGM
                     MRR"
     gene            complement(1917..2153)
                     /locus_tag="VFV20_05725"
     CDS             complement(1917..2153)
                     /locus_tag="VFV20_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5287884.1"
                     /translation="MTDEMPSARKRQVLALVAAGWPNKQIAARLGVTSWTIEKHLRQL
                     YRWYRVPNRAALVRVALRSGHLTERGIAAVEFDR"
     gene            2219..>2391
                     /locus_tag="VFV20_05730"
     CDS             2219..>2391
                     /locus_tag="VFV20_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007747794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU5287885.1"
                     /translation="MRHHHGVKRLRTVTLPGGDHVPVLGQGTWRMGERADQRKREIQA
                     LRTGIELGMTLIDT"
BASE COUNT          341 a          852 c          831 g          367 t
ORIGIN      
        1 gggcgttcgt gaaggtggcc tggatcggcg caccggtgcg cgccacgacc gccatcccga
       61 acgagccggc ctgccccgcc aggccggatc catcgtcgta cgcccagagc ggggtcggcg
      121 ggagctcggg atggagacgc cggctgatcg ggatctgcgt gaacgcgtag ttcgtccggc
      181 cggctcggag gcggctcgaa gtcgcgacga cgatgccggc gccggggacc gggagcggct
      241 cgaggtactt ggtgagcgcg ccgcgcatcg cggcgtcggc gacgtgtgcg ctcggtagcc
      301 acggagccac tgcggtcgtc gcccctgcgg ccgcggcgaa tttgaggagc gttcgacgtc
      361 tgagtgtgtg catcgcttac cccttcccca ggctggctct ctaccgaccg cgcaggtcca
      421 cgacgctaga cggccgggac cgaacgagac atcgagcaag cggggcgagt gctgtcatcc
      481 agcgcctgac acgcgtggtc accggatggt caccggcgat cacggcgaac tcatctacgc
      541 cagcagcaga tcctcagcgc tcctctgtga cgagggccgc tcccggagtt accgcctgtg
      601 ccgtccgccc gcgcgggccg ggcttggcga ggcggtcatc ggcggcggga cgtcttcgcg
      661 gtccccttcg gcttcgtctt cttcttcgtc gtcgtcttcg tcttcgccgg cttcctcttg
      721 gacttcgtcg cggctttctt cggacggacg cgctccggaa gatccttccg gcccgtcgag
      781 cgattccatt cctcgaacgt ctcgtccttg atcttgccct cgacgagcag atgggcgaac
      841 ttgcgccgct gcgccttgct cttgaacggc atgtcagcgc aaccctagcg cgtgctcgat
      901 ctcatcgaga tgctcgccgc gatgatcggc gcgcgaccag ttcaggagcg gcgtcgcctc
      961 gttcttcgcg atgatgtcgg ccgacagcgc ggcgacgcgt gcgtcgaccg cctcggcgat
     1021 ggcgaccgcg atctcggcgg ccttccgtgg cggcagcgcg agcaggagcg gcttcacgga
     1081 atcgttgatc cacgtgacat cagccgggag ctcttcgcgt ggggcaatgc ccttctccca
     1141 ctgctcgacg agcacgagga tgcgctggtc ccagaacgcc gcgtgcgcga gcacgcccgc
     1201 gacggtccag ccgccgggca tcggatgcgc gaggtccgca tcggtacatc tggcgaccag
     1261 cgcgacgaga cgcgcgcgct cgcggtcgtt ccccgcgacg tagcctctcg ccgtcgtcac
     1321 gcgaacgatt ctgcgccgct tgggcgtgcg tcgtgcggct ggaccctaca ggcatcggcc
     1381 ggaacgccgc tcgccgcctg acagcagagg actaggctgc gcgcgaccgt ggcgcatgcg
     1441 gcagccgtac agccccgccg cgacgtcctc ctccatcggg acgccctcac cgtcgctgtc
     1501 gccgccacgc tcttcgccgg cggggtcatg ttcgccgcgc tgtggggaac agcgcagctc
     1561 ctcgacacga gcgacatcgg gctcggatcg gcgctgagcg aggccgcgtt cgtcatcgag
     1621 cgcgacgcca acgcgctcgg cgccgaccgc gccgccgacg cgacgacgct gcggacgctc
     1681 gcggcgcggc tgcgcgagga cgcgcgcttc ctcggggagc atcccgagtg gaacaccaac
     1741 gcgagcgccg cactgctcgt cagcaaggtc gaccagctcg atgcggacgc gaacgcgctc
     1801 gcggcgcgcg gagccgcgct cggcgacgcg tccctcgtcg cggcggccga tcacatccgc
     1861 caggccgcac gcatggtgag cggtgccgcc gaacgcgcgc gcggaatgat gcggcgctag
     1921 cgatcgaact ccaccgccgc gatcccgcgc tccgtcagat gaccactccg tagggcgacg
     1981 cgcacgagcg ccgcgcgatt gggcacgcgg taccagcggt acagctgacg cagatgcttt
     2041 tcgatcgtcc aggacgtcac gccgagccgc gcggcgatct gcttgttcgg ccagcccgcg
     2101 gcgacgaggg ccaggacctg ccgcttgcgt gcgctgggca tctcgtccgt cattgcgcga
     2161 tggtcacgac gccacgacgg cacggcatgc cgcgaactga ccatcgacgg cccgccatat
     2221 gcggcaccat cacggcgtga agcggctgcg gaccgtgaca ctgccgggtg gcgaccacgt
     2281 cccggtgctc ggccagggga cgtggcggat gggcgagcgc gccgatcagc ggaagcgcga
     2341 gatccaggcg ctgcgcacgg gcatcgagct cggcatgacg ctcatcgaca c
//
LOCUS       DASTZR010000259         4138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1241577, whole genome shotgun sequence.
ACCESSION   DASTZR010000259 DASTZR010000000
VERSION     DASTZR010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241577"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VFV20_05735"
     CDS             complement(<1..261)
                     /locus_tag="VFV20_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287886.1"
                     /translation="MDPALAIVLSAGILGAAAVVVALLRRPPPATPATDAAPVIDLLR
                     SSVDELRGSTAAAIGEMRTELQRTLGATEQQLTTQTTSTQRAL"
     gene            342..1589
                     /locus_tag="VFV20_05740"
     CDS             342..1589
                     /locus_tag="VFV20_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5287887.1"
                     /translation="MEVRGPQRPLTFTESVVFGLGNFGANVVYNFLNGAAGLYLERYP
                     QVPTWAVGLLSQERSLAGAFVQPIVGAMSDRTRTPIGRRRPYFIVGVALTAASLLYLS
                     GFPPLIPMLMVLSINAFFLNVAVDPYTALLADLVPQDQRGRVGAVLAVFNMLGQVVAT
                     LAGFLLWNRSPELVFVIVAAVLVVSFGITTIFIHEPEAPPRPTEKVRIDIAAYARDLW
                     GRRELVKYIGAASLYWCGTGGVLPYLTRFGTHELGMSEGDAFLLLLPALGGTILGAVP
                     AGYVADRRGKKPVLVAGLFAYAVLAFIASQVQTVPQALVAMGFIGLANGIWTALAIPL
                     LVDLVPPERAAEMTGLGSAVWSLAQPIGAVIAGILIGSGDYRISFVGAAVFILASGVM
                     VSLVRAPRVSAQRQIGATAAPSE"
     gene            complement(2087..>4138)
                     /locus_tag="VFV20_05745"
     CDS             complement(2087..>4138)
                     /locus_tag="VFV20_05745"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEU5287888.1"
                     /translation="DAFREAYPDAAIVTLVENRRSSQGILDAAYRLIQHNPDRLEARL
                     NVEKHLKGRPASDLTEVDHHQYVSGADEAEEVANLIAREALVRQRRYGDFAILVRNNG
                     DATAFLNALARRQIPAHFSGGGQLYERPEIRLLISFLSAVALPSDSRHVYHLAVSALY
                     AFPAVELARATEASGRRQKALREVFEEIAVSEGAFTEEAVGSAKRLVEDLAYYAARAA
                     ELTTSELLYEFLDRSKLLAQYLEPESALAEEQGQNVAKFFRLVHSAARTLPTDRASFF
                     VPYLELLREAGDDPVAADFEASAERVNVLSIHKAKGLEFGVVFLVHATDERIPGSVRG
                     DELPLPPALAKSPAPTRDTHIAEERRLAYVAMTRAKDSFFFTSAVDYGGTRAHRPSRF
                     VAEALGRQPERLSARLAAYDELQRFQVAPAEADAPLPALGGDDILTVSFSDIDDYKRC
                     PLLYRFKHVLQIPVLPTPQMTYGLALHEAVRDYLRRKREGEKPTLRDLQATFRAAWLA
                     EGFISPEHETERFQAGLEALRRFHEQEKSQPAPELVEQRFSFMLGKDRVVGRWDRVDG
                     GPQGATVIDYKSTAIEEGSDKPQRTAQESFQLRLYALAHERMYGQRPASVVLHYLETG
                     ERGAIAPNDESVGAVRAWITSTAARIRAREFAASPAKGVRTCRECPYHQICPSSLTVR
                     D"
BASE COUNT          571 a         1438 c         1460 g          669 t
ORIGIN      
        1 gagcgcgcgc tgcgtcgacg tggtctgggt cgttagctgc tgctccgtcg cgcccagcgt
       61 gcgctgcagc tccgtgcgca tctcgccgat ggcggccgcg gtgctgccgc gcagctcatc
      121 cacgctcgaa cgcagcagat cgatcaccgg agcggcatcc gtcgctggcg tggccggcgg
      181 gggacggcgc aggagcgcca cgacgacggc agcggcgccc aggatgccgg cggagagcac
      241 gatcgcgagc gccggatcca tgacccctcc tcgctgaaca tgcgttcgat gtcgttcgag
      301 tctagtcgtg gcctccgcgt tcggctacaa cgccgcgcga catggaggtg cgtggaccgc
      361 agcgtcctct gacgttcacc gagagcgtcg tcttcgggct gggcaacttc ggggcgaacg
      421 tcgtctacaa ctttctcaac ggcgcggcgg gcctctacct cgagcgctac ccgcaggtcc
      481 ccacctgggc cgtcggcctc ctgagccagg agcgctcgct ggccggggcg ttcgtgcaac
      541 caattgtggg cgcgatgtcc gaccggacgc gaacgccgat cgggcggcgg cgaccgtatt
      601 tcatcgttgg cgtcgcgctg accgcggcct cgctcctcta cctctccggc ttccccccgc
      661 tcatcccgat gctgatggtg ctctcgatca acgcgttctt cctgaacgtc gcggtcgacc
      721 cctacaccgc gctgctcgcg gacctcgtgc cgcaggacca acgtggccgc gtcggcgcgg
      781 tcctcgcggt cttcaacatg ctcggccagg tcgtcgcgac cctcgccggc ttcctcctct
      841 ggaatcgctc gcccgagctg gtgttcgtca tcgtcgccgc cgtactggtc gtctccttcg
      901 gcatcacgac gatcttcatc cacgagccgg aggccccacc ccgtccgacc gagaaggtgc
      961 gcatcgacat cgccgcctac gcgcgggatc tctgggggcg ccgcgagctc gtgaagtaca
     1021 tcggtgccgc gtcgctctac tggtgtggca ccggtggcgt gctgccctac ctcacccgct
     1081 tcggcacgca cgagctcggg atgagcgaag gcgacgcgtt cctgctcctg ctcccggcgc
     1141 tcggcggcac catcctcggc gcggtgcccg cgggatacgt cgcggaccgg cgcgggaaga
     1201 agcccgtcct cgtcgcggga ctcttcgcct acgcggtgct cgcgttcatc gcgtcccagg
     1261 tgcagaccgt gccgcaggcc ctcgtggcga tgggcttcat cggcctcgcg aacggcatct
     1321 ggaccgcgct cgccatcccg ctgctcgtcg acctcgtccc gcccgagcgc gcggccgaga
     1381 tgaccggcct cggatcggcg gtgtggtcgc tcgcgcagcc gatcggcgcg gtcatcgccg
     1441 gcatcctcat cggcagcggc gactaccgga tctcgttcgt cggcgccgcg gtcttcatcc
     1501 tcgcgagcgg cgtgatggtg tcgctcgtcc gcgcaccccg cgtatctgca caaaggcaga
     1561 tcggagcgac ggccgcaccg agcgagtagc tcaagcgcgg cgtaccgatt tccgtccgag
     1621 gccttcttgg gcacgaccgc gacgccctcg tcacgctact cgacgacttc aacagaggcg
     1681 tggaccgccg cgaccgcctg ccgcgggagg gggacgttca agtccgacgc ggccttcgtc
     1741 ggacggagca tcgccgcgtc catgcgcggc ctcccccgtc gcgcctgcga ccgcgccttc
     1801 tgatgtaagc gcggtcgtcg tcaaggtcca ggggaacgcc ggacgcgcgt gagtacgagg
     1861 cgagagtcag gacctggcgc gtgttcgcgg gggcatccgc ggtcgcgcac ctggtgacgc
     1921 atcgtgacgc tccggctgtc gccacgccgc acgcgccaag gccggatcgt cggcgacggc
     1981 gagctcaccg gcgcggtcgt ccaacgcggt cgcgatcatc gcgtagtgga agaattggga
     2041 agcggacgat accgatattg gtatctgtct ttacgcaggg acgacgctag tcgcggacag
     2101 tgagtgagct gggacagatc tggtgatagg gacactcgcg acaggtgcgg acgcccttcg
     2161 ccggtgacgc ggcgaactcg cgcgcgcgga tgcgcgccgc cgtcgaggtg atccatgcgc
     2221 gtactgcgcc gacgctctcg tcgttcgggg cgatcgcacc gcgctcgccc gtttcgaggt
     2281 agtggagcac gacgctcgcg ggccgctgcc cgtacatgcg ctcatgcgcg agcgcataga
     2341 gccgaagctg gaagctctcc tgcgcggtgc gctgcggctt gtcgctgccc tcttcgatcg
     2401 cggtggactt gtagtcgatg acggtcgcgc cctgcgggcc gccgtccaca cggtcccacc
     2461 ggcccacgac ccgatccttg ccgagcatga aggagaagcg ctgttcgacg agctcgggtg
     2521 cgggctgtga cttctcctgc tcgtggaatc gacgcagcgc ctcgagcccc gcctggaaac
     2581 gctcggtctc gtgctccggg ctgatgaacc cctccgcgag ccacgccgcg cggaaggtcg
     2641 cctggagatc gcggagcgtc ggcttctccc cctcgcgctt gcggcgcagg tagtcccgca
     2701 ccgcctcgtg cagcgcgagc ccgtaggtca tctgcggtgt gggcagcacg gggatctgca
     2761 gcacgtgctt gaagcggtag aggagcgggc agcgcttgta gtcgtcgatg tcgctgaacg
     2821 acaccgtgag gatgtcgtcg ccgcccagcg cgggcagcgg cgcgtcggcc tccgcgggtg
     2881 cgacctggaa gcgctgcagt tcgtcgtacg ccgcgagccg ggccgagagg cgctccggct
     2941 gacggcccag cgcctccgcc acgaagcggc tggggcggtg cgcgcgggtg cctccgtagt
     3001 cgacggcgct ggtgaagaag aacgagtcct tcgcacgcgt catcgccacg taggcgagtc
     3061 gccgctcctc ggcgatgtgc gtgtcgcgcg tcggagcggg cgacttcgcg agcgcgggcg
     3121 gcagcggcag ctcgtcgccg cggaccgagc cggggatccg ctcgtcggtc gcgtgcacca
     3181 ggaacacgac gccgaactcc aggcccttcg ccttgtggat cgagagcacg ttcacgcgct
     3241 cggcggacgc ctcgaagtcg gcggcgaccg ggtcgtcccc cgcctcgcgc agcagctcga
     3301 ggtacgggac gaagaacgag gcgcggtcgg tcggcagcgt ccgcgccgcc gaatggacga
     3361 ggcggaagaa cttcgcgacg ttctggccct gctcctcggc gagcgcggac tcgggctcga
     3421 gatactgcgc gaggagcttc gagcggtcga ggaactcgta caggagctcg gaggtggtga
     3481 gctccgcggc acgcgcggcg taatacgcga ggtcctcgac caggcgcttc gccgacccga
     3541 cggcctcctc cgtgaacgcg ccctcggaca ccgcgatctc ctcgaagacc tcgcgcagcg
     3601 ccttttgccg gcgcccgctc gcctcggtcg cgcgcgcgag ctcgaccgcc gggaacgcgt
     3661 agagcgccga caccgccagg tggtagacgt gccgcgaatc cgagggcagt gcgaccgcgg
     3721 agaggaatga gatgaggagg cggatctcgg ggcgctcgta cagctgtccc ccgcccgaga
     3781 agtgcgcggg gatctgccgg cgggcgaggg cgttgagaaa ggcggtggcg tcgccgttgt
     3841 tccggaccag gatcgcgaag tcgccgtagc ggcgctggcg gacgagggcc tcgcgggcga
     3901 tgaggttcgc gacctcctcg gcctcgtcgg cgccgctcac gtactggtgg tgatcgacct
     3961 cggtcaggtc gctcgcgggc cgtcccttga ggtgcttctc gacgttgagc cgcgcctcga
     4021 gtcgatcggg gttgtgctgg atcaagcggt aggccgcatc gaggatgccc tgcgacgagc
     4081 gccggttctc gacgagcgtc acgatcgcgg cgtcgggata cgcctcgcgg aacgcgtc
//
LOCUS       DASTZR010000260         7350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_510501, whole genome shotgun sequence.
ACCESSION   DASTZR010000260 DASTZR010000000
VERSION     DASTZR010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7350
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510501"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1222
                     /locus_tag="VFV20_05750"
     CDS             <1..1222
                     /locus_tag="VFV20_05750"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5287889.1"
                     /translation="MPMPMRAVTLTAPTQAGSAPTATWFIPSGGVPVAVGDANDSVLS
                     RFDARAAPDGDSSVTVAHARVRAYAGPVRGAAGGRLVVAASMSAFDASLGGLAAVLGI
                     MGVAGIAIAVAVGWAISAGALRPIAKMSETARAIAVSRGFARRIEVRDEGRDELSELG
                     RTFNQMLASLDDAYRRQQRFVADVSHELRTPLTSIQGDLDLLTRGQLPPDEAAATLAE
                     ARRETRRLARLIDDLLVLARADAGPQPFAARPVQLDEVVMEVFREVRGQAGPRLGVTD
                     LDAALVQGERDRLKQLVLILVDNALSYTPPPGEVRISLRREAAETVLIVEDEGIGITV
                     ETAARAFERFYRGDEAERLDPGGTGLGLSIARWIVERHGGSISLEPKEGRGSRATVRL
                     PATQEHSLAAPAAA"
     gene            1288..1686
                     /locus_tag="VFV20_05755"
     CDS             1288..1686
                     /locus_tag="VFV20_05755"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU5287890.1"
                     /translation="MSPELVVPLAVVVIAVIGAAALWSCMRDGAVTEKARRLGDGTQE
                     ARVVVDQGYTPSRIELEAGVPTTLRFERRDDDACTEMLVSELWPSAHRLVGNGETAVR
                     FTPQRAGRYVFTCGMGMYSGELLVHEGRST"
     gene            1686..2165
                     /locus_tag="VFV20_05760"
     CDS             1686..2165
                     /locus_tag="VFV20_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287891.1"
                     /translation="MESAVRPAGVAAVALLGIALVSCAAPAVNTPAISAPAASTTARP
                     TVDPVAPLPEEQRHAAGAVTVIASWVPGAAAARVALDTHSVELDGFDLTALARVRLDG
                     ASWVSPTLWDAPKGGHHRSGTLTFGSLEPAQIAGARLIELEVRDVGASHLLRWQRAP"
     gene            2189..2638
                     /locus_tag="VFV20_05765"
     CDS             2189..2638
                     /locus_tag="VFV20_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287892.1"
                     /translation="MAGALGAAALVGLYLGIVTVAQGSAHAFELLWGDRVFVGLISAG
                     FGTQVGLFTYLRLLQRALARGSVALTGAGTTASSISMVACCAHHLADVLPIVGLSGLA
                     LFLVDLRTPLMLLGLAMNAIGILVMLGQLRRVTSGYRVVAHGEAATA"
     gene            2740..3315
                     /locus_tag="VFV20_05770"
     CDS             2740..3315
                     /locus_tag="VFV20_05770"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU5287893.1"
                     /translation="MRGPGAALLASLILMTACAAPAAPVTGSGPRTITIDMSDFGYSP
                     REISVRAGETLTLRFRNAGRIVHEFMAGRQPMAGIGYKEDWLAMAGGAHAGSHDTDHQ
                     GAGVRVEPSGSATLTITVPADAGEFEFACFVAGHYESGMKGRLVVTPSSAADTRPPAP
                     AASGAPARPAATPTATRMPDHTTGDMGDEAH"
     gene            complement(3339..3485)
                     /locus_tag="VFV20_05775"
     CDS             complement(3339..3485)
                     /locus_tag="VFV20_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEU5287894.1"
                     /translation="MAKDPVCGMDVDTKPGAKQSTYQGKTYYFCSDGCKQSFERDPGR
                     YAKA"
     gene            3593..3763
                     /locus_tag="VFV20_05780"
     CDS             3593..3763
                     /locus_tag="VFV20_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287895.1"
                     /translation="MTEVHIDGGHRAWYQACRCCLSDAHPEIVEALDRERAAAEQPPS
                     GTGICEAAEGEA"
     gene            complement(3787..5001)
                     /locus_tag="VFV20_05785"
     CDS             complement(3787..5001)
                     /locus_tag="VFV20_05785"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEU5287896.1"
                     /translation="MPGTTEAEVVRVSAAEARRLAITAQRLAGPRPRATRAGVLRLIE
                     HIGYLQLDPTNVVARNPYLVLWSRLGPYDPGLLDDLLARQRALFETPSLILPMSHLDI
                     HAATMREFRRVTAGRGWGPSGRGKWSGIATKWVPANAALRRSILTRLRREGPLPLTAF
                     EERAIVSWTSDGWDSPRNVALMLSILQRRGEVVVSGRRRGQKLWAIADGWLPKVKPLP
                     PTARAREAVLRSLHANGIATMRHLKRYYAFNRHITPAALASLERDGSVVRVRVEGVPG
                     TFFATNDIARRLRDVRAGWDGRTTLLSPFDNLIMDRDNAEALFGFFYRIEIYTPVRLR
                     KRGYWAMPVLHGDRIVGSVDPRMDRETGALVVNRVVLERDAPRSAMPAIHRAVGELAE
                     FTGARTVRWPRR"
     gene            5097..5303
                     /locus_tag="VFV20_05790"
     CDS             5097..5303
                     /locus_tag="VFV20_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEU5287897.1"
                     /translation="MATGTIKKLVGDRGFGFIQQADGSDLFFHRTAVVGTSFDALGEG
                     QAVTYEKTMDPRRNKESAEKVTPA"
     gene            5563..5967
                     /locus_tag="VFV20_05795"
     CDS             5563..5967
                     /locus_tag="VFV20_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287898.1"
                     /translation="MAVTVGRDAEVTRMLTSFQRDARAMLEQLLPLLHRPGLYPDAIV
                     DGWGDARGGRSTATTVVLMAPKGVRPTDRTKQLGRIRLEADATGTWTATVSIVAQPDE
                     SSPWPPASEPQWWAAASERFKTEVVRFRQTKA"
     gene            6036..6653
                     /locus_tag="VFV20_05800"
     CDS             6036..6653
                     /locus_tag="VFV20_05800"
                     /inference="COORDINATES: protein motif:HMM:NF025380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HEU5287899.1"
                     /translation="MFARIRMVQIAVGLTLAALAVLAMVLLLWRPFDRDTTLRYTPSV
                     VVAVRDLARLESVEYHVERVIDLRDQQSLLFGLIRTQDAILFVAVGQVIAGVDLGELS
                     DGDVVVDQSRSTARITLPAARVLSTRLDNDHSWVYARTTDALAQRHEDLETRARREAE
                     RTLEASAVSGGILARARENAERTVAAFVRSLGYDTVIVRSRDALP"
     gene            6719..>7350
                     /gene="yqeB"
                     /locus_tag="VFV20_05805"
     CDS             6719..>7350
                     /gene="yqeB"
                     /locus_tag="VFV20_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HEU5287900.1"
                     /translation="MLTLGEQRVIVKGAGDLATGVAYRLHQSGFTVIMTEIAQPTVVR
                     RSVAFAQAVFDGTSVVEGVQARLVTTSDEALNVARTHEIAVVVDPGAQIVRRLRPAVL
                     VDAIIAKRNTGTRMTDAPIVVGLGPGFRAGVDVHAVVETNRGHRLGRVILDGEAEPDT
                     GVPAPVDGHGADRVLRAPADGVFVGTRPIGDRVVAGSIVGYVGDDPVRST"
BASE COUNT         1047 a         2622 c         2629 g         1052 t
ORIGIN      
        1 gatgccgatg ccgatgcgcg ccgtcacgct gaccgctccc actcaggcgg gcagcgcgcc
       61 gaccgcgacg tggttcatcc catcgggcgg cgtgccggtc gccgtcggcg acgcgaacga
      121 cagcgtgctg tcgcgcttcg atgcgagagc ggcaccggac ggggacagca gcgtgacggt
      181 cgcgcacgcc cgcgtgcgcg cctacgccgg cccggtccgc ggcgcggcgg gcggacgtct
      241 ggtggttgcc gcgtcgatgt ccgcgttcga tgcgtcgctc ggcgggctcg ccgccgtgct
      301 cgggatcatg ggcgtggccg gcatcgccat cgcggtcgcc gtcggctggg cgatctccgc
      361 gggcgcactg cggcccatcg cgaagatgtc ggagaccgcg cgggccatcg ccgtgtcacg
      421 cggattcgca cgcaggatcg aggtgcgcga cgagggccgg gacgagctca gcgagctcgg
      481 ccgcacgttc aatcagatgc tcgcgagcct cgacgacgcg taccgtcgac agcagcgctt
      541 cgtcgccgac gtgtcgcacg agctgcgcac gccgctcacg tcgatccagg gcgatctcga
      601 cctgctcacg cgcggacagc tgccgcccga tgaggccgcg gccacgctgg cggaggcgcg
      661 gcgagagacc cgccgcctcg cccggctgat cgacgatctc ctcgtgctcg cacgggccga
      721 tgccggcccg cagccattcg cggcgcgacc ggtccagctc gacgaggtgg tgatggaggt
      781 cttccgcgag gtccgcggtc aggccgggcc gcgcctgggc gtgaccgacc tcgacgcggc
      841 gctggtgcag ggcgagcgcg atcgcctcaa gcagctcgtc ctcatcctcg tggacaacgc
      901 gctcagctac acgccgccgc cgggtgaggt gcgcatctca ctgcggcgcg aggcggcgga
      961 gacggtgctg atcgtcgagg acgaagggat cggcatcacc gtggagacgg cggcccgcgc
     1021 gttcgagcgc ttctaccgcg gcgacgaggc cgagcgcctc gaccccggcg gcacgggcct
     1081 cgggctgtcg atcgcgcggt ggatcgtcga gcggcacggt ggatcgatct cgctcgagcc
     1141 aaaggagggg cgcgggtcgc gtgccaccgt ccgcctcccc gcgacgcagg agcacagcct
     1201 cgcggcgccc gccgccgcgt agcttcctca gcgccacctc agcgcgctcc cagagacgcg
     1261 taagggcggc tcgggaccat gggcgccgtg agcccggagc tcgtcgtccc gctcgcggtg
     1321 gtcgtcatcg ccgtgatcgg cgcggccgct ctgtggtcct gcatgcgcga cggcgcggtg
     1381 accgagaagg cgcgccgtct cggcgacggg acgcaggaag cacgcgtcgt ggtggatcag
     1441 ggctatacgc cgtcgcgcat cgagctcgag gcaggcgtcc cgacgacgct ccgcttcgag
     1501 cgccgcgacg acgacgcgtg taccgagatg ctcgtctccg agctgtggcc gagcgcgcac
     1561 cggctcgtcg gcaacggtga gaccgccgtg cgcttcacgc cgcagcgcgc cggccgctat
     1621 gtcttcacgt gcgggatggg gatgtactcc ggcgagctgc tcgtacacga agggagatcg
     1681 acctgatgga atccgcggtg aggcccgcgg gcgtcgccgc ggtcgcgctc ctcggcatcg
     1741 ctctcgtctc gtgcgccgca ccggccgtga acacgccggc catcagcgca ccagccgcga
     1801 gcacgaccgc gcggccgacc gtagaccccg tcgcgccgtt gcccgaggag cagcggcatg
     1861 cggcgggcgc cgtcaccgtg atcgcctcat gggtgcccgg ggcggcggcc gcacgcgtcg
     1921 cgctcgacac gcactccgtc gagctcgacg gcttcgatct gacggcgctc gcgcgcgtcc
     1981 gcctcgacgg cgcttcgtgg gtgagcccca cgctctggga cgcgccgaag ggcgggcacc
     2041 accggtcggg aacgctgacc ttcggatcgc tcgagcccgc gcagatcgca ggcgcgcggc
     2101 tcatcgagct cgaggtccgc gacgtgggcg cgtcgcatct ccttcgctgg cagcgcgcgc
     2161 cgtgagccgg cgcatcgctg tcgccacctt ggccggagcg ctcggtgccg ccgcactcgt
     2221 cgggctctat ctcggcatcg tcacggtggc gcagggaagt gcgcacgcgt tcgagctgct
     2281 ctggggagac cgcgtgttcg ttggactcat cagcgccggc ttcggcacgc aggtcggcct
     2341 cttcacgtac ctgcgactcc tgcagcgcgc cctcgcgcgc ggatccgtcg cgctcacggg
     2401 tgcgggcacg acggcgtcga gcatctcgat ggtcgcgtgc tgcgcgcacc acctcgccga
     2461 cgtgctgccg atcgtcggac tgtccgggct ggccctcttc ctcgtcgatc tccgaacacc
     2521 gctgatgctt ctgggtctcg cgatgaacgc gatcgggatc ctggtgatgc tggggcagct
     2581 gcgccgcgtc acgagcggct accgggtcgt cgcgcacggc gaggccgcga cggcctgagg
     2641 ggcacgcgat ccgtgcgcgt tcgccggcgt cccgcacttc cccgatgaca gcgcggcgac
     2701 gcaccggcat gatgaggccc gcgacggagg tctgcgtcca tgcggggacc gggagcggca
     2761 ctgctcgcga gcctcatcct gatgacggcc tgcgcggcgc ccgccgcgcc ggtcaccggc
     2821 agcggcccgc gcaccatcac gatcgatatg agcgacttcg gctactcgcc gagggagatc
     2881 tcggtcaggg cgggcgagac cctgaccctg cgcttccgga acgccggccg gatcgtgcac
     2941 gaattcatgg cgggccggca gccgatggcc ggcatcggtt acaaggagga ctggctcgcc
     3001 atggccggcg gcgcgcacgc tggtagccat gacaccgacc accagggcgc cggggtccgc
     3061 gtcgagccga gcggatccgc gaccctgacg atcacggtgc ccgcggatgc cggcgagttc
     3121 gagttcgctt gcttcgtcgc gggacactac gagagcggca tgaagggcag gctcgtcgtc
     3181 acgccgtcga gcgcggccga cacgcggcca ccagcgccgg cggcgagtgg cgcgccggcg
     3241 cgtcctgcgg caacgccgac ggccaccagg atgcctgatc acacgacggg cgacatgggg
     3301 gacgaggcgc actagccgcc cgcgtgaatg ctcgccggtc aggccttcgc gtaccgcccc
     3361 gggtcacgtt cgaaggactg cttgcagcca tcgctgcaga agtagtaggt cttgccctga
     3421 taggtcgact gcttcgcgcc gggcttcgtg tcgacatcca ttccgcagac gggatccttc
     3481 gccatgctgg tcacctccga gctcgtcgcc gcgggtcgta ggatggccct cgactcgtgg
     3541 ctgcaggata cgttccgaac atgtcggctt cgcgcgtgaa ggagagagca agatgacgga
     3601 ggtccacatc gacggcgggc accgcgcctg gtaccaggca tgccgctgct gcctttccga
     3661 cgcgcacccc gagatcgtcg aggcgctcga ccgcgagcgc gcggccgccg agcagccgcc
     3721 gtccggcacc ggcatctgcg aggccgcgga aggagaggcg tgacggctcg ccgctcccgc
     3781 tgacgcttag cggcgcggcc agcgcaccgt ccgcgccccg gtgaactcgg cgagctcgcc
     3841 gaccgcgcga tggatcgcgg gcatggcgct ccgcggcgcg tcgcgctcga gcacgacgcg
     3901 gttcacgacc agcgcgcccg tctcgcggtc catccgcgga tcgacgctcc cgacgatccg
     3961 gtcgccgtgg aggaccggca tcgcccagta gccgcgcttc cgcagacgca cgggtgtgta
     4021 gatctcgatg cgatagaaga agccgaagag cgcttcggcg ttgtcccgat ccatgatcag
     4081 gttgtcgaag ggcgagagca gcgtggtgcg tccgtcccag cccgcccgca cgtcgcgtag
     4141 ccgccgcgcg atgtcgttcg tcgcgaagaa ggtgccgggc acaccctcga cccgcacgcg
     4201 gacgacgctc ccatctcgct cgagtgacgc gagcgcggcg ggcgtgatgt ggcggttgaa
     4261 ggcgtagtac cgcttgagat gccgcatcgt cgcgatccca ttcgcgtgga gcgagcgcag
     4321 caccgcctct ctcgcgcgcg ccgtcggcgg aagcggcttc accttcggga gccagccatc
     4381 ggcgatcgcc catagcttct gaccgcggcg ccgaccgctg acgacgacct cgcctcggcg
     4441 ctgcaggatc gacagcatca gcgcgacgtt gcgtggactg tcccagccat ccgaggtcca
     4501 gctcacgatg gcccgctcct cgaacgcggt gagcggcagg ggcccctcgc gccggagacg
     4561 cgtcaggatg ctgcgacgga gcgcggcgtt cgccggcacc cacttcgtgg cgatgccgct
     4621 ccactttccg cggcccgacg ggccccaccc gcgtcccgcc gtcacgcgcc tgaactcccg
     4681 catcgtcgcg gcgtggatgt cgagatggct catcggaagg atgagcgacg gcgtctcgaa
     4741 gagcgcgcgc tgccgtgcga gcaggtcgtc gaggagcccg ggatcgtagg gaccgagacg
     4801 gctccacagg acgaggtacg gattccgcgc cacgacgttc gtcgggtcga gctggaggta
     4861 cccgatgtgc tcgatcagac ggagcacgcc cgcgcgcgtc gcgcgtggcc gggggcctgc
     4921 gaggcgctgc gcggtgatcg ccaggcggcg cgcttcggcg gcgctcacac gaacgacctc
     4981 tgcctctgtc gtccccggca cgcgcctgag cgtacggaaa gaggatgacg aggggctaca
     5041 gtggagtttc caggcatcgc acgtcgcggc gtacagccgt gacgaggaga aacagagtgg
     5101 ctacgggaac tatcaagaag ctggtcggcg accgcgggtt cggattcatc cagcaggccg
     5161 acggctcgga cctgttcttt caccgcacgg cggtggtggg cacgagcttc gacgccctcg
     5221 gcgagggcca ggccgtgacc tacgagaaga cgatggatcc gcgtcggaac aaggaaagcg
     5281 ccgagaaggt cacgccggct tagcgccggc ggccttgagc aaggacacct tcgcctggat
     5341 cacggcgcgc gacggtagcg tccacgcgac ccgaccgccg atcaacgccg atggtcagac
     5401 gatgctctgc ggtcagctct acgaccggcg cgatctccgc atcaggtccc tcgggacgga
     5461 cgccaacgct ccgcccgacg catgtccgac ctgccgcgac cgcgccgccg cgggctcgac
     5521 cgcagagccg ggtgccccgc gtcccgagcg caaggcgccg atgtggccgt gacggtcggc
     5581 cgggacgccg aggtcacccg gatgctgacc tctttccagc gcgacgcgcg cgccatgctc
     5641 gaacagctgc ttccactcct tcaccggccg ggcctctacc ccgacgccat cgtcgacgga
     5701 tggggcgacg cgcgcggcgg tcggtcgacg gcaacgacgg tcgtgctcat ggcgcccaaa
     5761 ggcgtgcgtc cgaccgatcg caccaagcag ctcggacgga tccgtctcga ggcggacgcg
     5821 accggaacgt ggacggcgac ggtctcgatc gtcgcgcaac cggacgagag ctccccgtgg
     5881 ccgccggcgt cggagcccca gtggtgggcc gccgcgagcg agcgcttcaa gaccgaggtc
     5941 gtgcgcttcc gccagacgaa ggcctgaacc ccgccggaga cgcgcgggcg tgcggtcggg
     6001 tgggttggca gcggtcatgc ggtcgccagg cgtttgtgtt cgcacgcatc cgcatggtcc
     6061 agatcgccgt gggcctcacg ctcgcggcgc tcgccgtgct cgcgatggtg ctgctgctct
     6121 ggcgtccctt cgaccgcgac accacgctgc ggtacacacc cagcgtcgtc gtggcggtcc
     6181 gggacctcgc gcggctcgag agcgtcgagt accacgtcga gcgcgtcatc gatctgcgcg
     6241 accagcagtc gctcctcttc ggcctcatca ggacgcagga cgccattctc ttcgtcgcgg
     6301 tcgggcaggt catcgcgggc gtggacctcg gcgagctgtc cgacggtgac gtcgtggtcg
     6361 atcagtcacg gagcaccgcc cggatcacgc tgccggccgc gcgcgtgctc tcgacacgcc
     6421 tcgacaacga tcacagctgg gtctacgcgc gcaccacgga cgcgctcgcg cagcgccacg
     6481 aggacctcga gacgcgcgcc cgccgcgagg cggagcgcac gctcgaggcg agcgcggtct
     6541 cgggtgggat cctcgcgcga gcgcgcgaga acgcggagcg gaccgtcgcc gcgttcgtgc
     6601 gttcgctcgg ctacgacacg gtgatcgtgc gatcccgcga cgcgctgccc tgacccgatc
     6661 cccggccgac ctgaggactg agatacgact gacacagtgc gttcggatgc tcgtcgtgat
     6721 gctcacgctg ggcgaacagc gcgtgatcgt gaagggggcg ggcgacctcg ccaccggcgt
     6781 ggcctaccgt ctccatcagt cgggcttcac cgtgatcatg accgagatcg cacaaccgac
     6841 cgtcgtccgg cgcagcgtcg ccttcgcgca ggcggtgttc gatggaacga gcgtcgtcga
     6901 gggagtccag gcgcggctcg tgaccacctc cgacgaggcg ctcaacgtcg cacgcacgca
     6961 cgagatcgcc gtcgtcgtgg atcccggcgc gcagatcgtc cgccgcctgc ggcccgccgt
     7021 cctcgtcgac gcgatcatcg ccaaacggaa caccggcaca cggatgacgg acgcacccat
     7081 cgtcgtcggc ctcggccctg ggttccgggc cggcgtggac gtgcacgccg tcgtcgagac
     7141 caatcgcggt caccggctcg gtcgcgtgat cctcgacggc gaggcggagc cggacaccgg
     7201 cgtccccgcg ccggtcgacg gccacggtgc cgatcgcgtg ctgcgcgcgc ccgccgacgg
     7261 cgtgttcgtc ggtacgcgtc cgatcggtga ccgagtcgtg gcgggttcga tcgtcggcta
     7321 cgtcggcgac gaccccgtgc gcagcacgtt
//
LOCUS       DASTZR010000261         3041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_350876, whole genome shotgun sequence.
ACCESSION   DASTZR010000261 DASTZR010000000
VERSION     DASTZR010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350876"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VFV20_05810"
     CDS             complement(<1..782)
                     /locus_tag="VFV20_05810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HEU5287901.1"
                     /translation="MITSVTLNPAVDEAIAVEEIALGEINRCDLDAVDPGGKGLNASR
                     VIARLGRRTRAFGFVAGVTGELIRARLDAEGVPHAFDDVPGLTRINVMVLERRHARRT
                     RLYLPGPHVGAADVARLRTRLETCASGETVIIGGSVPPGLDAGVYGDLVRWLRGRGVR
                     TVVDASGAALESALAAQPDLIKPNVEEAVALLGRPLASDDEVCDAARSLRALGAARVV
                     ISQGAHGAIGVDEAGAWKVRAPAVLVRTTVGAGDSMVAGLAIA"
     gene            complement(779..1369)
                     /locus_tag="VFV20_05815"
     CDS             complement(779..1369)
                     /locus_tag="VFV20_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5287902.1"
                     /translation="MHARRRIVLTGGPGAGKTAVLELVRQTFCEHIRVLPESAGIVYG
                     GGFPRGVDLELRRASQRAIFHVQRELEAAGDIGAPPVVLCDRGTVDGGAYWPGPGELW
                     PAVGTTRADELARYDCVIHLRVPRGTVDRGNPLRVESPLEAQLIDDRIAALWDGHPRR
                     FTVAAESDFLDKAARAVALLRHEVPECCRPRALVLA"
     gene            1519..>3041
                     /locus_tag="VFV20_05820"
     CDS             1519..>3041
                     /locus_tag="VFV20_05820"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HEU5287903.1"
                     /translation="MTEAFALVLHSHLPYARGAGRWPHGEEWVHEAILGTYLPLLGLL
                     HDLRDEGVPYAITVGLTPTLLEQLADADIDHRFVEYADDQIRRADQDLHRFGGDGETE
                     RGALALFYASLFRAQREAYVRRFGRDIVGAFADLARTGHVEILTSGATHGYLPLLDPA
                     SVHAQLEVGTRTTRRRTGVEPRGIWLPECAYAPGLEEILESFGITHFFTDAALLGGRR
                     LVEQGHRFAARRSGDAIAAPLATAADIDVLRPYLVRDSHVAAVARHDTVSGQVWSAMM
                     GYPGDPAYREFHRKDERSGMRYWRVTDVHVSLSGKSTYSPGHASERVRVHAEHFAGLV
                     RETLAARRHPDRPALLTVTFDAELFGHWWFEGVEWLGRVLRELAARGPRPVSVRDHLR
                     ADPARERVALQEGSWGKDNDHSTWANDRTMWMWTDLAKMAGEMQDLRAVPASGQMRER
                     AARQAARELLLAQSSDWPFLVTTGQAADYAVERFRSHAMRFRRAAELARRATAGDEV"
BASE COUNT          413 a         1132 c         1079 g          417 t
ORIGIN      
        1 gcgatcgcga ggcccgcgac catcgagtca cccgcgccca ccgtcgtgcg tacgaggacc
       61 gccggcgccc gcaccttcca cgcgccggct tcgtcgacgc cgatggcacc gtgtgcgccc
      121 tgcgagatca cgacacgtgc cgcgccgagc gcgcgcagcg aacgcgcggc gtcgcacacc
      181 tcgtcgtcgc tcgcgagcgg ccgtcccagg agcgcgaccg cctcctcgac gttcggcttg
      241 atcaggtcgg gctgcgccgc gagcgcgctc tcgagcgccg cgcccgacgc gtcgaccacg
      301 gtgcgcacgc cgcggccgcg gagccatcgc acgagatcgc cgtacacgcc ggcgtcgaga
      361 ccgggcggca cgctgccgcc gatgatcacc gtctcgcccg acgcgcatgt ctcgagccgt
      421 gtgcggagcc gcgcgacgtc ggcggcaccg acgtgcgggc cggggagata gaggcgcgtg
      481 cgccgcgcat gcctccgctc gagcaccatg acgttgatcc gtgtgaggcc cggcacgtcg
      541 tcgaacgcgt ggggcacgcc ctcggcatcg aggcgcgcac ggatgagctc gccggtgacg
      601 ccggcgacga agccgaaggc ccgcgtccgc cgcccgagcc gcgcgatgac gcggctcgcg
      661 ttcagcccct tgccacccgg atcgaccgcg tcgagatcgc agcggttgat ctcgccgagc
      721 gcgatctcct cgacggcgat cgcctcgtcc accgccgggt tcagcgtgac gctggtgatc
      781 acgcgagcac gagcgcgcgt ggacggcagc actctggtac ctcgtgacga aggagcgcga
      841 ccgctcgcgc cgccttgtcg aggaagtcgg actcggccgc gacggtgaag cggcggggat
      901 gaccgtccca cagcgccgcg atgcgatcgt cgatgagctg tgcttccagc ggcgactcga
      961 cacggagcgg attgcctcga tccaccgtgc ctctgggcac gcgcaggtgg atcacgcagt
     1021 cgtaccgcgc gagctcgtcg gcgcgcgtcg tcccgaccgc gggccacagc tcaccgggtc
     1081 ccggccagta cgcgccgccg tccacggtcc cgcgatcgca cagcacgacc ggcggcgcgc
     1141 cgatgtcgcc cgcggcctcg agctcgcgct gcacgtggaa gatcgcgcgc tgggacgcgc
     1201 gccgcagctc gaggtccacg ccgcgcggaa agccgccgcc gtagacgatg ccggccgact
     1261 ccggcagcac gcgtatgtgc tcgcagaagg tctggcgcac gagctccagc accgcggtct
     1321 tgccggcgcc gggtccgccg gtgaggacga tccgacggcg cgcgtgcacg agatcgcacg
     1381 ggcatggcct catctgtctt ctcccggcga cacgctagga gcgcgacctt ccgcccgcgt
     1441 gtgccgaacg gtcgcccgcg gacatgccga aggtccgccg ggacgcttgg agcgggctgt
     1501 gcgggtacag tcgagacggt gaccgaggcc ttcgcgctcg tgctgcattc tcatctgccc
     1561 tacgcgcgcg gcgccgggcg ctggccgcac ggtgaggagt gggtgcacga ggccatcctc
     1621 ggcacgtacc tgcctctcct cggcctgctc cacgacctgc gcgacgaggg cgtgccgtac
     1681 gcgatcacgg tcggcctcac cccgacgctc ctcgagcagc tcgccgacgc ggacatcgat
     1741 catcggttcg tcgagtacgc cgacgatcag atcaggcgcg ccgaccagga cctgcatcgc
     1801 ttcggcggcg atggcgagac cgagcggggc gcgctcgcgc tgttctacgc ctcgctcttc
     1861 cgcgcgcagc gtgaggcgta cgtccggcgt ttcgggcgcg acatcgtcgg cgcgttcgcg
     1921 gacctcgcgc gtaccggtca tgtggagatc ctcacctccg gtgcgacgca cggctacctt
     1981 ccgctgctcg atcccgcgtc ggtgcacgcg cagctcgagg tcggcacgcg cacgacgcgc
     2041 cgccgcaccg gcgtcgagcc acgcggcatc tggctccccg agtgcgcgta cgcgcccggg
     2101 ctcgaggaga tcctcgagtc gttcggcatc acgcacttct tcaccgacgc cgcgctcctc
     2161 ggcggacgcc ggctcgtcga gcagggccat cgcttcgcgg cgcggcgctc cggcgacgcg
     2221 atcgcggcgc cgctcgccac cgcggccgac atcgacgtgc tccgtccgta tctcgtgcgc
     2281 gactcgcacg tcgctgccgt cgcgcgccac gacacggtct cagggcaggt gtggtccgcg
     2341 atgatgggct atccgggcga tcccgcgtat cgcgaattcc atcgcaagga cgagcggagc
     2401 gggatgcgct actggcgcgt gaccgacgtg cacgtgagcc tctccgggaa atcgacgtac
     2461 tcgcccggcc acgcctcgga gcgcgtgcgc gtgcacgccg agcacttcgc gggcctcgtg
     2521 cgcgagacgc tcgccgctcg gcggcatccg gaccggccgg cgctgctgac ggtcaccttc
     2581 gacgcggagc tctttggcca ctggtggttc gagggcgtcg aatggctcgg ccgcgtgctg
     2641 cgcgagcttg ccgcgcgcgg tccacgaccc gtgagcgtgc gcgaccatct gcgcgccgat
     2701 cccgcgcgcg agcgcgtcgc gctgcaggaa gggtcatggg gcaaggacaa cgaccactcg
     2761 acctgggcca acgaccgcac gatgtggatg tggaccgacc tcgcgaagat ggccggtgag
     2821 atgcaggatc tgcgtgccgt cccggcgagc ggccagatgc gcgagcgcgc cgcccgccag
     2881 gccgcgcgcg agctgctgct cgcgcagtcg agcgactggc cgttcctcgt gacgacagga
     2941 caggccgccg actacgcggt cgagcggttc cggagccacg cgatgcgttt ccgccgcgcc
     3001 gcggagctcg cgcgccgcgc gaccgcgggc gacgaggtgg a
//
LOCUS       DASTZR010000262         3861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_846998, whole genome shotgun sequence.
ACCESSION   DASTZR010000262 DASTZR010000000
VERSION     DASTZR010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846998"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2183
                     /locus_tag="VFV20_05825"
     CDS             <1..2183
                     /locus_tag="VFV20_05825"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU5287904.1"
                     /translation="SVPGVVSAEVNLANERATVRLDPATAQLPAIVQAVARAGYGALV
                     IPQGDAARAAAVEDERGLRLRYVESLRRRLVVASVLAGGAVLLSMVLPFIFPALEAAA
                     WRPYVLFALATPVQLWAGSIFYRNAWNAAKHLTSNMNTLIAVGTTAAYGISVVATFFP
                     DLFSNNALDPNASMYYETSSAIIALVLAGRFMEARARAHTGDAIRALLALGAKTARVR
                     RPGGLEEDVPVEQLRIGDVVLVRPGEIVAMDGLVLAGASAVDESMLTGESIPVEKGAG
                     DEVTGGTLNTTGSLRFRVTRIGQDTTLAQIVRLVEDAQASKAPIQRLVDQISAIFVPI
                     VFVVATAALVAWLLFGPDPKMSYALNAFVAVLIIACPCALGLATPTAIMVGTGRGAMR
                     GILIKSAIALEAAHRVDTVAFDKTGTLTIGRPQVTDILACADYGEDESLRFVASAEMR
                     SEHPLAGAVLEAAREKGLTPSEPETFESFPGQGIHARVDGHEVYVGNRAFAVAHGFAE
                     LGDGLLAHHESQGKTPLIATIDGKPLAILAVADVPRAAAADAVAELKKMGLRTLLVSG
                     DTRRTAEAVARTLGIDEVRAEVRPDAKASIVSELQRAGRKVAMVGDGVNDAPALTQAD
                     LGIAIGSGTDVAIASAGFVLVGGDPRGVPRALRLARKTVGTIRTNLFWAFFYNVALIP
                     LAAGLFYPFLGVLLSPILAAGAMAISSVTVVTNSLRLRGATIDK"
     gene            2201..2545
                     /locus_tag="VFV20_05830"
     CDS             2201..2545
                     /locus_tag="VFV20_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEU5287905.1"
                     /translation="MTTPVKMLDPICDMVVEVAGARDQGLTIERDDREYAFCSHGCLV
                     KFSKAPQQYVPKVEAWLAAGAHEAAHEHREELPMIDNGMREWYKSCRCCLSEAYPKVV
                     ETLDAEKAAGGV"
     gene            complement(2556..2795)
                     /locus_tag="VFV20_05835"
     CDS             complement(2556..2795)
                     /locus_tag="VFV20_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287906.1"
                     /translation="MPDLVWSALLIAGIVVLGALLGGGRTREDMRTRRRWDAAFDHES
                     RRVSRTDATGMLRCRSCGASASERAGRCPSCGALL"
     gene            complement(2795..3547)
                     /locus_tag="VFV20_05840"
     CDS             complement(2795..3547)
                     /locus_tag="VFV20_05840"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEU5287907.1"
                     /translation="MAVQLGRLEVETSDHVVLRYDLAGGGNRGFAAIVDFVIATLVLA
                     GALWGFTTLAAATRGAIGQFFGVLVLVAFAIAWSYFVLLEWLWQGQTVGKRMYGLRVI
                     RDDGAPAGFIAVLIRNLLRIVDFLPALYGLGLLMVIVTPRSQRLGDMAAGTYVVRAPR
                     PQLDYFSLRTMTPVAEGASVETRALPGEAQRLVREFVAREARLATADRTRLARQIAER
                     VRPYAREAATIEDDVEFLRAVARSLRASAGGR"
     gene            3571..>3861
                     /locus_tag="VFV20_05845"
     CDS             3571..>3861
                     /locus_tag="VFV20_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEU5287908.1"
                     /translation="MTIDRFVEDRRARWARLGQLISVARGRVDRLSADDVLELGHLYR
                     ATTSDLAIARRDFPRDVATERLNDLVAAAHAIVYSEAPTSGRRLRGFLLRELP"
BASE COUNT          576 a         1409 c         1355 g          521 t
ORIGIN      
        1 agagcgtccc cggcgtcgtg tccgcggagg tcaacctcgc gaacgagcgg gcgaccgtcc
       61 gcctcgaccc cgccacggcg cagctcccgg cgatcgtgca ggcagtcgca cgcgccggct
      121 acggcgcgct ggtcatccca cagggtgacg ccgcgcgtgc ggccgcggtc gaggacgagc
      181 gcgggttgcg tcttcgctac gtcgagtcgc tgcgccggcg tctggtcgtc gcgagcgtgc
      241 tcgccggcgg cgcggtcctc ctgtcgatgg tcctgccgtt catcttcccg gccctcgagg
      301 ccgcggcatg gcgtccctac gtgctcttcg ctctcgcgac gccggtacag ctctgggccg
      361 gctcgatctt ctaccgcaac gcgtggaacg cggcgaagca cctcaccagc aacatgaaca
      421 cgctcatcgc cgtcggcacg accgccgcgt acggcatctc cgtcgtggcg acgttcttcc
      481 ccgacctctt ctcgaacaat gcgctcgacc cgaacgcgag catgtactac gagacctcga
      541 gcgcgatcat cgcgctcgtc ctcgccggcc ggttcatgga ggcgcgcgca cgcgcccaca
      601 ccggcgacgc gatccgcgca ctactcgccc tcggcgccaa gaccgcgcgc gtgcggcgtc
      661 cgggcggcct cgaggaggac gtcccggtcg agcagctccg gatcggcgac gtcgtgctcg
      721 tccgccccgg cgagatcgtc gcgatggacg gcctcgtcct cgccggcgcg tccgccgtcg
      781 acgagtcgat gctgaccggc gagtcgatcc cggtggagaa gggcgccggt gacgaggtca
      841 ccggcgggac gctgaacacc accggcagcc tgcggttccg cgtcacgcgc atcggccagg
      901 acaccacgct cgcgcagatc gtccgcctgg tggaggacgc gcaggcgtcg aaggcgccga
      961 tccagcgcct cgtcgaccag atctccgcga tcttcgtccc gatcgtcttc gtcgtcgcga
     1021 ccgccgccct cgtcgcatgg ctgctgttcg ggccagaccc gaagatgagc tacgcgctca
     1081 acgcgttcgt tgcggtgctc atcatcgcgt gcccgtgcgc gctcggcctc gcgacaccca
     1141 cggcgatcat ggtgggcacc ggccgcggcg cgatgcgcgg catcctcatc aagagcgcga
     1201 tcgcgctcga ggcggcccac cgcgtggaca ccgtcgcgtt cgacaagacg ggaacgctca
     1261 ccatcggccg gccgcaggtc accgacatcc tcgcgtgcgc ggactacggc gaggacgagt
     1321 cgctccgctt cgtcgcgagc gcggaaatgc gctccgagca tcccctcgcg ggcgcggtcc
     1381 tcgaggccgc acgcgagaaa ggcctcacgc cgagcgagcc cgagacgttc gagtcgttcc
     1441 ccggccaggg catccacgcg cgggtggacg gacacgaggt gtacgtcggc aaccgcgcgt
     1501 tcgcggtcgc gcatggcttc gccgagctcg gcgacggcct cctcgcgcat cacgagtcgc
     1561 aggggaagac accgctcatc gcgaccatcg acggcaaacc gctcgcgatc ctcgcggtcg
     1621 ccgacgtgcc gcgcgcggcg gcggcggacg cggtcgcgga gctgaagaag atgggccttc
     1681 gcacgctcct cgtgtccggc gacacgcggc ggacggccga ggccgtcgca cggacgctcg
     1741 gcatcgacga ggtccgcgcc gaggtgcgcc cggacgcgaa ggcgagcatc gtgtccgagc
     1801 tccagcgagc cggccggaag gtcgcgatgg tgggcgatgg cgtgaacgac gcgccggccc
     1861 tcacgcaggc cgacctcggc atcgcgatcg gcagcggcac cgacgtcgcg atcgcgtcgg
     1921 cgggcttcgt gctcgtcggc ggcgacccgc gcggcgtgcc gcgcgccctg cgcctcgcgc
     1981 gcaagaccgt gggcacgatc cgcacgaacc tgttctgggc gttcttctac aacgtcgcgc
     2041 tcatcccgct ggcggccggc ctgttctacc cgttcctcgg cgtgctgctg tcgccgatcc
     2101 tcgcggcggg cgcgatggcg atctcctcgg tcaccgtggt cacgaattca ctgcgcctgc
     2161 gcggcgccac gatagacaaa tagttgaagg gaagacgacg atgacgactc cggtgaagat
     2221 gctggatccg atctgcgaca tggtggtgga ggtcgcgggc gcgcgcgacc aggggctcac
     2281 gatcgagcgc gacgaccgcg aatacgcctt ctgcagccac ggctgcctcg tgaagttctc
     2341 gaaggcaccg cagcagtacg tcccgaaggt ggaggcgtgg ctcgccgccg gcgcccacga
     2401 ggccgcgcac gagcaccgcg aggagctgcc gatgatcgat aacggcatgc gcgagtggta
     2461 caagtcgtgc cgctgctgcc tcagcgaggc gtacccgaag gtcgtcgaga cgctcgacgc
     2521 ggagaaggcc gccgggggcg tttagcccga aggcatcaca gcagcgcgcc gcacgacgga
     2581 cagcggccgg cccgctccga agcgctggcg ccgcacgagc ggcagcgcag catgccggtc
     2641 gcatcggtgc gcgagacgcg ccggctctcg tgatcgaagg ccgcgtccca gcgccgccgc
     2701 gtgcgcatgt cctcacgcgt ccgaccgcct ccgaggagcg cgccgaggac gacgatgccg
     2761 gcgatgagca gcgccgacca gacgaggtcc ggcatcagcg accacccgcc gaggcgcgaa
     2821 gcgaacgcgc gaccgcgcgc aggaactcga cgtcgtcctc gatcgtcgcg gcctcccgcg
     2881 cgtacggccg cacgcgctcc gcgatctgcc gggcgagacg ggtgcgatcg gccgtcgcga
     2941 gcctcgcttc gcgtgcgacg aactcgcgga cgagccgctg cgcctcgccg gggagcgcgc
     3001 gcgtctcgac ggacgcgccc tcggccaccg gcgtcatcgt gcggagagag aagtagtcga
     3061 gctgcggtcg cggcgcgcgg acgacgtacg tgcccgcggc catgtccccg aggcgctgtg
     3121 agcgcggcgt cacgatcacc atcaggagtc ccagcccgta gagggcgggc aggaagtcga
     3181 cgatccgcag gaggttgcgg atgaggacgg cgatgaatcc ggcgggcgcg ccgtcgtcgc
     3241 ggatcacgcg gagcccgtac atccgcttcc ccaccgtctg gccctgccag agccactcga
     3301 gcagcacgaa gtacgaccac gcgatcgcga acgcgaccag gacgagcact ccgaagaatt
     3361 ggccgatcgc gccgcgcgtc gccgccgcga gggtcgtgaa gccccagagc gcgccggcga
     3421 gcacgagcgt ggcgatgacg aaatcgacga tcgcggcgaa tccgcgattg ccgccgcccg
     3481 ccaggtcgta gcggagcacg acgtggtcgc tggtctcgac ctccagccgg ccgagctgga
     3541 ccgccatacg ggcatggtac ggtcgcggtc gtgacgatcg accggttcgt ggaggatcgg
     3601 cgcgcacgct gggcacgcct cggccagctg atctcggtgg cgcgcggccg ggtcgatcgc
     3661 ctctccgctg acgacgtgct cgagctcggc cacctctatc gcgccacgac ctcggacttg
     3721 gcgatcgcgc gccgcgactt cccgcgcgac gtggccaccg agcggctcaa cgacctcgtc
     3781 gccgcggcac acgccatcgt gtacagcgag gcgccgacga gcgggcgccg cctccgcgga
     3841 ttcctgctgc gcgagctgcc c
//
LOCUS       DASTZR010000263         5257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_745572, whole genome shotgun sequence.
ACCESSION   DASTZR010000263 DASTZR010000000
VERSION     DASTZR010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745572"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VFV20_05850"
     CDS             <1..317
                     /locus_tag="VFV20_05850"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEU5287909.1"
                     /translation="EDYARAGVPMLPVVRGDEATTWGIFTYALSLIPLSLLLFVAGGL
                     GLIYLVAAIVLGVLFVLFAVRLLRAAAARRRAMARGIYMYSLLYLALLFVAIMVDSSL
                     RL"
     gene            340..1149
                     /locus_tag="VFV20_05855"
     CDS             340..1149
                     /locus_tag="VFV20_05855"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEU5287910.1"
                     /translation="MESVAIFLAVLTGAVYARGVRLAPRRWRWWRTLCFFLGLAAVVL
                     ALASGIQVLAGDLFSVHMLQHMLLTTVAAPLLMVGAPVRPLLRGLPVAVRARIVRPLA
                     GAPAIRTLFHVLRHPLVAVLIYVGGLYLWHWPALYDAAVEDEALHVLEHAHFFIGALL
                     FWSVVIDPEPFKGTLPYAARIVYLLLAGAAQNTILGGLLSFSTRVLYAHYERAAPLRG
                     IDALTDQRVGGAIMWVPGDTIFLIAASVAFFMWLEQEEREQRRREQGNLRA"
     gene            1146..1565
                     /locus_tag="VFV20_05860"
     CDS             1146..1565
                     /locus_tag="VFV20_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287911.1"
                     /translation="MTPRLAALFCALALLVASCGSSAPATLPADVQVTIDMKDYSVTV
                     TPSTFKAGTIKFGVRNLGAMAHQFDLIKTDLAADKLPVDNASAKAKEDGLVKQLLNIP
                     PGKVATISGDVQPGHYVIICNVAGHYQLGMRAEFKAE"
     gene            complement(1610..2563)
                     /gene="coxB"
                     /locus_tag="VFV20_05865"
     CDS             complement(1610..2563)
                     /gene="coxB"
                     /locus_tag="VFV20_05865"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEU5287912.1"
                     /translation="MGPWPNDPLGPQARASADVYWIMFVCAIIVLAIVDGGLIYAGLR
                     FRDRPGHIAKQFHGHNFLELVWTVVPTLMVISFSVLSFQKLNFMNDTSNAGMTVEVQG
                     QQWTWLMNYPKEDQFKLNDGSYLQGATELHIPVDTKVHILLSSKDVIHSFWVPNIGGK
                     KDAVPGRKTDLWIQADRPGTYKGQCFEFCGTGHADMLITLVVHTPEDYAAWAKSAVAE
                     AQRGSDPATKKGRETFLALPCVGCHTVQGTTAQGKVGPDLTHVASKKNIAGGAVSPVN
                     EDTLTRWIKNPPALKPNTQMPNLGLSDEQVHDIVQWLLTLK"
     gene            2624..2857
                     /locus_tag="VFV20_05870"
     CDS             2624..2857
                     /locus_tag="VFV20_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287913.1"
                     /translation="MAGHHGTSGPTAEHEIHLPGPSISPALLGLGVTILAFGILFGLP
                     LIILGLAIFLIGLVTWLIDDARAYAHAPDEGHH"
     gene            2857..3543
                     /locus_tag="VFV20_05875"
     CDS             2857..3543
                     /locus_tag="VFV20_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEU5287914.1"
                     /translation="MRTDVQERPVVRGRRERPRDRLPVNDTGYDSQPARFTSGMWAVI
                     LFVSSEAMFFGALFTSYFYLRGRLPEWEPVFQRCADICEKPTFAVDPIFGIGLPTLNT
                     IELIISSITMQLAVNAIKKGDRVALRNWLIPTLALGVLFLIGQGYEYTQLGFLPDNGV
                     FAAVFFTLTGFHGAHVTGGVLMNAYVFLRTQRGHFTANRHLAVEAASIYWHFVDVVWI
                     GLFTTIYIIG"
     gene            complement(3626..4192)
                     /locus_tag="VFV20_05880"
     CDS             complement(3626..4192)
                     /locus_tag="VFV20_05880"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEU5287915.1"
                     /translation="MRLLCGALAIALIACSAPARLSGTALDGGAAPDFTLTDGISGSA
                     VTLSSLRGSVVVIAFLYTHCPDVCPITASKFRAAQTALGPDAARVRFVAVSVDPEGDT
                     PNDVRAFSAAHDLSTNWVYLIGPRAQLEPVWKSYGVGAFASPMAGHGVDHNDAIYVLD
                     ANGRERELVHSDIAVNELVNDLRALLRG"
     gene            4226..5140
                     /locus_tag="VFV20_05885"
     CDS             4226..5140
                     /locus_tag="VFV20_05885"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU5287916.1"
                     /translation="MPPVGVVVRYVVLVLLWGSTWAAIRIGVTAVPPFVFAFERALAV
                     SVLLTAASVALGLRFPRDGRTLLAAAIAGVVNTGSSWAIIFWSEQYVPSGLVSVFGAA
                     GPIWTAILAHFLVHGDRLSRMKIAGLVLGLAGIGVLVGAPDPGSRPEALVATVLLALM
                     PITWAIGAIVQVRVLREGSAVPLVAVGTWTGSLVLAPLALVQANTAASWTIEVIAAFA
                     YLVVLGSCVGLVLQLWLIRRLRPTTMSLSQLPISAMALTVGAVVLGESITWRMLVGAG
                     LVGVALVLNALSGGGRTEDVATGTAAAD"
BASE COUNT          706 a         1850 c         1758 g          943 t
ORIGIN      
        1 gcgaggatta cgcgcgcgcc ggcgtgccga tgctccccgt ggtgcgcggc gacgaggcga
       61 ccacctgggg gatcttcacc tacgcgctct cgctgatccc gctctcgctg ctgctcttcg
      121 tggccggggg tctggggctc atctacctcg tcgcggcgat cgtgctggga gtgctgttcg
      181 tcctgttcgc ggtccggttg ctgcgcgccg cggccgcgcg gcggcgggcg atggcgcgcg
      241 ggatctacat gtactcgctc ctgtacctcg cgctgctctt cgtcgcgatc atggtggact
      301 cgtcactcag gctgtagacc ggcctcgagg cgtggctcga tggaatccgt agcgatcttc
      361 ctcgcggtcc tcaccggtgc cgtctacgcg cgcggcgtgc gtctggcgcc gcgccgctgg
      421 cgctggtggc ggacgctgtg cttcttcctc ggcctcgccg cggtcgtcct ggccctcgcg
      481 tcggggatcc aggtcctcgc cggcgacctc ttcagcgtcc acatgctcca gcacatgctg
      541 ctcaccaccg tcgccgcccc gctgctcatg gtcggcgcgc cggttcgccc gctgctgcgc
      601 ggactgcccg tcgccgtacg cgcgcggatc gtgcgcccgc tcgcgggcgc gccggcgata
      661 cggacgctct tccacgtgct ccgccacccg ctcgtcgccg tgctgatcta cgtcggcggt
      721 ctgtacctct ggcactggcc ggcgctgtac gacgcggccg tcgaggacga ggcgcttcac
      781 gtgctcgagc acgcgcactt cttcatcggc gcgctgctct tctggagcgt ggtcatcgac
      841 cccgagccgt tcaagggcac cctgccgtac gccgcgcgca tcgtgtacct gctgctcgcc
      901 ggtgcggcgc agaacacgat cctcggcgga ctcctctcgt tctcgacgcg cgtgctgtac
      961 gcgcactacg agcgagcggc accactgcgt ggcatcgacg ctctcaccga ccagcgcgtg
     1021 gggggcgcga tcatgtgggt ccccggcgac accatctttc tcatcgcggc ctcagtcgcg
     1081 ttcttcatgt ggctggagca ggaggagcgc gagcagcgcc ggcgcgagca gggtaatctc
     1141 agggcatgac ccctcgcctt gccgcgctgt tctgcgcgct tgccctgctc gtcgcgtcat
     1201 gcgggtccag tgcgcccgcg acgctccccg cggacgtcca ggtcacgatc gacatgaagg
     1261 actattcggt gacggtcacg ccgtccacct tcaaggcagg cacgatcaag ttcggggtgc
     1321 ggaacctcgg cgcgatggcc caccaattcg atctcatcaa gaccgacctc gcggcggaca
     1381 agctgcccgt cgacaacgcg agcgcgaagg cgaaagagga tggtctcgtg aagcagctgc
     1441 tgaacatccc gcccggaaag gtcgcgacga tcagcggcga tgtccagccc ggacactacg
     1501 tgatcatctg caacgtcgcg ggccactatc agctcgggat gcgcgcggag ttcaaggccg
     1561 aatagcgcga cgagcctcga ccgcgtcagc ggtcgggatg ccgccccctc tacttgagcg
     1621 tgagcagcca ctggacgatg tcgtgcacct gctcgtccga caggccaagg ttgggcatct
     1681 gggtgttcgg cttcagcgcc ggcgggttct tgatccaccg cgtcagcgtg tcctcgttca
     1741 ccggcgagac cgcgccgccc gcgatgttct tcttgctcgc gacgtgcgtg aggtccggcc
     1801 cgaccttgcc ctgcgccgtc gtgccctgca cggtgtggca gccgacgcac ggcagcgcga
     1861 ggaacgtctc gcgtcccttc ttcgtcgccg ggtcgctccc gcgctgcgcc tcggccaccg
     1921 cgctcttcgc ccacgccgcg tagtcctcgg gtgtgtgcac gaccagcgtg atgagcatgt
     1981 cggcgtggcc cgtgccgcag aactcgaagc actggccctt gtacgtcccg ggcctgtccg
     2041 cctggatcca gaggtcggtc tttcgaccgg ggaccgcgtc cttcttgcca ccgatgttcg
     2101 gcacccagaa ggagtggatc acgtccttcg aggacaggag gatgtgcacc ttcgtgtcca
     2161 cggggatgtg cagctcggtg gcgccctgca ggtacgagcc gtcgttgagc ttgaactggt
     2221 cctctttggg gtagttcatg agccaggtcc actgctggcc ctgcacctcg acggtcatgc
     2281 cggcgttgct cgtgtcgttc atgaagttca gcttctggaa ggacaggacc gagaagctaa
     2341 tgaccatgag cgtcggcacg acggtccaga ccagctcgag gaagttgtgt ccgtggaact
     2401 gtttcgcgat gtggccgggc cggtcgcgga agcgcagacc ggcgtagatc agcccgccat
     2461 cgacgatggc gagcacgatg atcgcgcaga cgaacatgat ccagtagacg tctgcggagg
     2521 cccgcgcctg cgggcccaag gggtcgttcg gccacggtcc catgcgtcgc caagactagg
     2581 gccagatttc gacacatgcc aatcgcgccc atacactgcc gccgtggcgg gacaccacgg
     2641 gacgagcggc ccgacggccg aacacgagat ccatctgccc ggaccgagca tctctccggc
     2701 gctcctcggg ctgggcgtca ccatcctcgc gttcggcatc ctcttcggcc tgccgctcat
     2761 catcctgggc ctcgcgatct tcctgatcgg cctcgtcacc tggctcatcg acgacgcgcg
     2821 cgcctacgcg cacgcgccgg acgagggcca ccactgatgc ggaccgacgt ccaggagcgc
     2881 ccggtcgtcc gcggccgccg cgagcggccc cgcgatcgcc tccccgtcaa cgacacgggc
     2941 tacgactcgc agcccgcgcg gttcacgtcg gggatgtggg cggtcatcct cttcgtgtcg
     3001 tcggaggcga tgttcttcgg cgcgctgttc acctcgtact tctacctccg cgggcggctc
     3061 ccggaatggg agccggtgtt ccagcgctgc gccgacatct gcgagaagcc gacgttcgcg
     3121 gtcgacccga tcttcggcat cggactcccg acgctcaaca ccatcgagct gatcatctcg
     3181 agcatcacca tgcagctggc ggtgaacgcc atcaagaagg gggaccgcgt cgcgctgcgc
     3241 aactggctca tccccacgct cgcgctcggc gtcctgttcc tcatcggcca gggctacgag
     3301 tacacgcagc tcgggttcct ccccgacaac ggggtcttcg ccgcggtctt cttcacgctc
     3361 accggattcc acggcgcgca cgtgaccggc ggcgtgctca tgaacgcgta cgtgttcctg
     3421 cgcacccagc gcggccattt caccgcgaac cggcatctcg cggtcgaggc ggcgtccatc
     3481 tactggcact tcgtggacgt cgtctggatc ggcctcttca cgacgatcta catcatcggc
     3541 tgaccctcag ccgatgaacg tatggacgcg gcggaggccg cgtccatcca gcatcgggtg
     3601 atcttcaccc ggaccgcctg gaccgttagc cccgtagtag cgcgcgcagg tcgttcacga
     3661 gctcgttcac cgcgatatcg gagtgcacca gctcgcgctc gcggccgttc gcatccagca
     3721 cgtagatggc gtcgttgtgg tccacgccgt gaccggccat cggggacgcg aacgctccga
     3781 ccccgtagct cttccacacc ggctccagct gagcgcgcgg cccgatgagg tagacccagt
     3841 tcgtcgagag gtcgtgcgcc gcggagaagg cgcgcacgtc gttcggggtg tcgccctccg
     3901 gatcgacgga gacggcgacg aagcgcacgc gcgccgcgtc cgggccgagc gcggtctgcg
     3961 cggcgcggaa cttcgacgcg gtgatggggc agacgtccgg gcagtgcgtg tagaggaacg
     4021 cgatcacgac gacgctcccg cggagcgacg acagcgtgac cgcgctcccg ctgatgccgt
     4081 cggtgagcgt gaagtccggc gcggcgccgc cgtccagcgc cgttcccgag agccgcgccg
     4141 gcgctgagca cgcgatgagc gcgatcgcga gcgcgccgca cagaagacgc atcacgctcc
     4201 gaggatatgc gcgagtatcg acggcatgcc ccccgtcggt gtcgtcgtcc gctacgtcgt
     4261 gctcgtcctc ctgtggggat cgacgtgggc ggccatccgc atcggtgtca cggccgtgcc
     4321 gccattcgtc ttcgcgttcg agcgggccct cgcggtctcg gtgctgctca ccgccgcgtc
     4381 ggtcgcgctg gggctgcgct ttccgcgcga cggccgcacg ctcctcgccg cggcgatcgc
     4441 cggtgtggtg aacaccggct cgagctgggc gatcatcttc tggtccgagc agtacgtgcc
     4501 gagcgggctc gtttcggtct tcggcgccgc gggtccgatc tggacggcga tcctcgcgca
     4561 cttcctcgtg cacggcgatc ggctgtcgcg gatgaagatc gccggactcg tgctcggcct
     4621 cgccgggatc ggtgtgctcg tcggcgctcc cgatcccggc tcgagaccgg aggcgctcgt
     4681 cgcgaccgtg ctgctcgcgc tcatgcccat cacctgggcg ataggcgcga tcgtccaggt
     4741 gcgcgtgttg cgcgaaggct ccgcggtccc gctcgtggcg gtcgggacgt ggaccggcag
     4801 cctcgtcctc gcgccgctcg cgctcgtgca ggcgaacacg gcggcgtcct ggaccatcga
     4861 ggtcatcgcg gccttcgcct atctcgtcgt gctcggatcg tgcgtcgggc tggtgctcca
     4921 gctgtggctc atccgccggc tgcggcccac gacgatgtcg ctctcgcagc tcccgatctc
     4981 ggcgatggcc ctcaccgtgg gcgcggtggt gctgggcgag tcgatcacgt ggcggatgct
     5041 ggtgggggcg ggtctggtcg gcgtcgcgct cgtgctgaac gcgctctccg gcggagggcg
     5101 gaccgaggac gtcgcgaccg gcacggccgc ggccgattga tcattcgttc gacctcgaga
     5161 tcaccgctcg cttcccggcc cgctcgctga ggatccgggc gcccatgcgg gcgatgcgag
     5221 cgggccatgg agtctcctac gacagcgtcg tagtatt
//
LOCUS       DASTZR010000264         6200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_666171, whole genome shotgun sequence.
ACCESSION   DASTZR010000264 DASTZR010000000
VERSION     DASTZR010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666171"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VFV20_05890"
     CDS             complement(<1..185)
                     /locus_tag="VFV20_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5287917.1"
                     /translation="MRRLRLDPILVACWFCAFTPILDTNIVNLAIPRIGMAFGTTASE
                     LAWVVTGYVLPFAVSILA"
     gene            complement(198..1004)
                     /locus_tag="VFV20_05895"
     CDS             complement(198..1004)
                     /locus_tag="VFV20_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014752981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5287918.1"
                     /translation="MTLLEARGIVAGYGGREVLRGCDLVIDAGEIVAVVGPNGAGKST
                     LLKVCAGLLRPAGGAVRIAGDPIAALSRAEIARRVAVVPQAIETLFPFTVRELVALGR
                     TARLDALGRGSHDDEAAIARALGTLDLDGLAERRVDAISGGERQRVVLAMALAQETPV
                     LLLDEPTVHLDPAHQLATIDLLRRLASERGLAVLAVVHELNLAALADRVVVLAAGRVA
                     RSGAPPEVIEQELVRAVFGGGWSVTTHGGRPFVLPEAATRDGMDARTRLG"
     gene            complement(1001..1951)
                     /locus_tag="VFV20_05900"
     CDS             complement(1001..1951)
                     /locus_tag="VFV20_05900"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU5287919.1"
                     /translation="MSAVAGVLFGTVPLPFGSTFDALLGRGDATTGTIVRDLRLPRVL
                     AAGLVGGALAVSGALLQGLLRNPLADPFVTGASAGGALGAIAAVALAASAPGAIVPVA
                     SFAGSLIAVALVWRMARIGGPTTVLTVLLAGVVLTSFAGAVITLVVVASDRLSLRLRA
                     LLDVLIGGVSVRDGAELLVAAIVIGVAVAAALALAPRVDAYAFGEETAATLGVDTERV
                     TAGVLVVTALLAGAAVALSGLVAFVGLVVPHAVRGVLGARHRPLVAASFLAGAAVLIC
                     ADLGARIALAPAELPVGVITGAVGGPFFLLLLVRSRRVVA"
     gene            complement(1996..2943)
                     /locus_tag="VFV20_05905"
     CDS             complement(1996..2943)
                     /locus_tag="VFV20_05905"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEU5287920.1"
                     /translation="MTRPVLAILAVLALLLAACGPSATATVSASPSIAPTTAATPSAF
                     PVTVADFQGRSVSVASRPSRVVSVGPSITEFLFALGAGDRVVGVDDFSDQPAAAAKLE
                     KVGGVKVNIEKVVALRPDLVLSVKFSDGTIERLAGAGIPVLVVDPQTIGDVTRTAILV
                     GRAVGADGERLALQIDQRLQQVRAAVAGATTKPRVFHEVDASDPAKPFTVGPGSYIHD
                     LIAIAGGVNVAANAGSAYPQISLEEIVRADPEVIVLGDADYGTTVDQVGARPGWSGMA
                     AVRNKKVFPISDSLVSRPGPRVGDAAEAYARLLHPEVFR"
     regulatory      complement(2944..3086)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01482"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="The adenosylcobalamin (AdoCbl) riboswitch is found
                     in the 5'-untranslated regions of vitamin B12 (Cobalamin)
                     related genes in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF01482"
     gene            complement(3101..3367)
                     /locus_tag="VFV20_05910"
     CDS             complement(3101..3367)
                     /locus_tag="VFV20_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287921.1"
                     /translation="MYSSLISKIEKAKRYAEEPERITFETFDVRFRGDNGSHRVSLRG
                     DDWTCECDHFHTTGLCAHVMTMQRVFASHLSEAARYTTEHVVPA"
     gene            complement(3579..4046)
                     /locus_tag="VFV20_05915"
     CDS             complement(3579..4046)
                     /locus_tag="VFV20_05915"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEU5287922.1"
                     /translation="MPTRIDGRDRPTCPVCGFADFMHVQIGANTIVEREDGVLLVRLN
                     YGPRDGRWALPGGLVEADETPVEAAIRETEEETGFSVELDGLLATWMRPGFPIIVVIY
                     RAHITAGELRVAPDEASEARFFPREHLPPIEELAWPSTAHGLDAWRAYEPPRP"
     gene            complement(4161..5237)
                     /locus_tag="VFV20_05920"
     CDS             complement(4161..5237)
                     /locus_tag="VFV20_05920"
                     /inference="COORDINATES: protein motif:HMM:NF006426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HEU5287923.1"
                     /translation="MASTAPDTSIIASVERIRAADPGNMLDRIKDLAKQVRDAWRIAN
                     AAALPPAYADVRNITVAGMGGSAIGGDLAAALLADELKIPMSVHRDYGAPGYVGRDSL
                     VIASSYSGNTEETLSAFDAVRQRGAKILVLTTGGRISELAKAGGYPTVTFAYKAQPRA
                     ALGYSLGLVLGCLCRLGFVRDLAADIEAALADLAKLEERVHEGASTNDAKKLAIDLYG
                     RIPFAYGAGVLGVMARRVKGQFNENAKNWSAFDVMSELNHNAVVGFAHPPAAREALTV
                     LLLRSDRDNPRHKIRLDVTAELLDRANIRHTTLRFTGQNMLSEVLQMTMFTDYVTFYL
                     ALLNGADPSEVRSIDYLKDRLAKS"
     gene            complement(5230..>6200)
                     /locus_tag="VFV20_05925"
     CDS             complement(5230..>6200)
                     /locus_tag="VFV20_05925"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HEU5287924.1"
                     /translation="FDPAPDYLARVGELFDLEAFRAAGYTIVCEPLFGSASGYFPRLL
                     GGGRTQIVELHGERNPFFGGVNPEPIPPNIDEFLARIPAEHANVGLAVAGDADRAGLG
                     DEKGNFVTTLTVYALLMWYLLEVRKLRSPVVKTVNMTSMCDRLGEKYGVPVHEVPVGF
                     KYIGPKMQETGAMMGGEESGGFGFAMHLPERDGIVADLFLLDFMHQTKKTPSQLIGEL
                     MAEFGPSFYLRRDLHQEGATYAKKKIEIMDRLSAAQPRELAGHAVKKVVPLDTKDGVK
                     FFVDDGSWLLVRLSGTEPLVRVYTETRAQQEVKPMLDAGERMVTGG"
BASE COUNT          949 a         2177 c         2228 g          846 t
ORIGIN      
        1 gcgaggatcg agacggcgaa cggcagcacg taaccggtca cgacccaggc gagctccgat
       61 gccgtcgtcc cgaaggccat gccgatccgc gggatggcga ggttgacgat gttcgtgtcg
      121 aggatcggcg tgaaagcgca gaaccagcag gcgacgagga tcgggtcgag gcgcaggcgg
      181 cgcatgaacg tgcgtttcta ccccaatcgg gttcgcgcgt ccatcccgtc gcgcgtcgct
      241 gcctccggaa ggacgaaggg ccggccacca tgcgtcgtaa cggaccaacc gccgccgaag
      301 acggcgcgca ccagctcctg ttcgatgacc tccggcggcg cgcccgaacg cgcgacgcgg
      361 cccgcggcga gcacgacgac gcggtccgcg agcgcggcga ggttcagctc gtgcacgacc
      421 gcgaggaccg cgaggccgcg ctcggacgcg agccggcgaa ggaggtcgat cgtcgcgagc
      481 tgatgcgctg gatcgaggtg caccgtgggc tcgtcgagca gcagcaccgg cgtctcctgc
      541 gcgagcgcca tcgcgagcac gacgcgctgg cgctcgccgc cgctgatcgc gtcgacgcgc
      601 cgctcggcga ggccgtcgag gtcgagtgtg cccagcgcgc gcgcgatggc agcctcgtcg
      661 tcatgggacc cccgtccgag ggcgtcgagc cgcgccgtcc ggcccagcgc gacgagctcg
      721 cggacggtga acgggaacag cgtctcgatg gcctgcggca cgacggcgac gcgccgcgcg
      781 atctcggcgc gggacagcgc ggcgatcggg tcaccggcga tgcgcaccgc tccgccggcc
      841 ggacgcagca ggccggcgca gaccttgagc agcgtcgact tgcccgcgcc gttcggcccg
      901 acgaccgcga cgatctcgcc cgcatcgatc acgaggtcgc acccacgcaa cacctcgcgt
      961 ccgccgtagc cggccacgat gcctcgcgcc tcgagcagcg tcacgcgacg acccgccgcg
     1021 agcgcaccag gagcaggagg aagaagggac cgcccaccgc gccggtgatg acccccaccg
     1081 gcagctccgc gggcgcgagc gcgatgcggg cgccgaggtc cgcgcagatg agcaccgcgg
     1141 ccccggcgag gaacgacgcc gcgaccagcg ggcggtgacg cgcgccgagc acgccgcgca
     1201 ccgcgtgcgg cacgacgagg cccacgaacg cgacgagccc cgacagcgcg accgccgcgc
     1261 ccgcgaggag cgcggtcacg acgagcacgc ccgcggtcac gcgctccgtg tcgacgccca
     1321 gcgtcgcggc ggtctcctcg ccgaacgcgt acgcatcgac gcgcggcgcc agtgccagcg
     1381 cggccgcgac ggcgacccca atgacgatgg cggcgacgag cagctcggcg ccgtcgcgca
     1441 ccgacacgcc gccgatgagc acgtcgagga gggcgcgcag ccggagcgag agacggtcgc
     1501 tcgccacgac gacgagcgtg atcaccgcac cggcgaacga cgtcagcacc actccggcga
     1561 ggagcaccgt gagcacggtc gtcggcccgc cgatgcgcgc catccgccac accagcgcca
     1621 ccgcgatgag cgagccggcg aacgacgcga ccggcacgat cgctcccggc gccgacgccg
     1681 cgagcgcgac cgccgcgatc gccccgaggg cgccaccggc ggacgcaccc gtgacgaacg
     1741 ggtccgcgag gggattgcgc agcagtccct ggaggagcgc gccggacacg gccagcgcgc
     1801 cgccgacgag gccggccgcg agcacgcgcg ggaggcgcag gtcgcgcacg atcgttccgg
     1861 tcgtcgcgtc gccgcgtccg agcagcgcgt cgaacgtcga tccgaatggg agcgggacgg
     1921 tgccgaagag gacccccgcc acggcgctca cgacgagcgc cgcggcgagg atcgcgataa
     1981 cggcggtcgt gcgcgtcagc ggaagacctc cggatggagc agcctcgcgt acgcctccgc
     2041 cgcatcgccg acgcgcggcc cgggtcgcga cacgaggctg tcgctgatcg ggaacacctt
     2101 cttgttcctc accgccgcca tgccggacca gcccggccgc gcgccgacct ggtcgaccgt
     2161 cgtcccgtag tcggcgtcac ccaggacgat gacctcggga tcggcgcgga cgatctcctc
     2221 gaggctgatc tgcggatagg ccgagcccgc gttcgcggcg acgttcacgc cgccggcgat
     2281 cgcgatgagg tcgtggatgt atgagcccgg tcccacggtg aacggcttcg ccgggtcgct
     2341 cgcgtcgacc tcgtggaaca cgcgcggctt ggtggtggcc ccggcgacgg cggcccgcac
     2401 ctgctggagg cgctggtcga tctggagcgc gagccgctcg ccgtcggccc cgaccgcccg
     2461 gccgacgagg atggcggtgc gcgtgacgtc gccgatggtc tgcggatcaa cgacgagcac
     2521 cgggatgccc gcgccggcga ggcgctcgat cgtgccgtcg gagaacttca cgctgagcac
     2581 gagatcgggc cggagcgcaa cgaccttctc gatgttcacc ttgacgccgc cgaccttctc
     2641 cagcttcgcg gcggcggccg gctggtcgga gaagtcgtcg acgccgacga cgcggtcgcc
     2701 ggcacccagt gcgaagagga attcggtgat cgacggtccg acggagacga cgcggctcgg
     2761 ccgggacgcg acgctgaccg agcgcccctg gaagtcggcg accgtgacgg ggaacgcgga
     2821 gggcgtagcg gcggtggtgg gagcgatcga gggagatgcg gagacggtgg cggtcgcgga
     2881 tggtccgcac gcggcgagga ggagcgcgag gacggcgagg atcgcgagga ccgggcgggt
     2941 catcaggcgc acccctttca cgcgaaggtg tgtgggccgc gagcgggatg cccaggcgac
     3001 ccgagttcgg gcgtgagccc tttcacggtg gcgcgaccct gccggaatct caccggcttc
     3061 gctgatgcac ccgcgtattc agttgtgggt ccgcgaacgg ctaggccggg acgacgtgct
     3121 ccgtcgtgta ccgggcagcc tcgctcaggt ggctcgcgaa gacacgctgc atggtcatga
     3181 catgtgcgca caggccggtc gtgtggaagt gatcacattc acacgtccag tcatccccgc
     3241 gaagagacac gcgatgcgag ccgttgtcgc cacggaatcg gacgtcaaag gtctcgaagg
     3301 tgatccgctc gggctcttcg gcataacgct tcgccttctc gatcttggag atgaggctcg
     3361 agtacatgcg actcctccac cactccgtct ttgccggctg gcgccggtct gccgattcta
     3421 ggcgcgcccc atttctgacg cgcgggtgag gcggcgcgcc ctgcgagcgt tactgaaagc
     3481 ctctgtacgc gggcctctcc cgtcgctgca cgcgaccgcg tcgccggccc ctattcgcgg
     3541 cttggtgcgc gctgagtaag ggcgcgagcg ggcggcggct acgggcgggg cggctcgtag
     3601 gcgcgccagg cgtccagccc gtgggcggtg gagggccacg cgagctcctc gatcggcggg
     3661 agatgctccc gcgggaagaa gcgggcctcg gaggcctcgt ccggggcgac acgcagctcc
     3721 ccggcggtga tgtgcgcgcg gtagatcacg acgatgatcg gaaagcccgg ccgcatccac
     3781 gtggcgagga gcccgtcgag ctcgacgctg aagccggtct cctcctcggt ctcacggatc
     3841 gccgcctcca cgggcgtctc gtcggcctcg acgagcccgc cgggcagcgc ccagcgtccg
     3901 tcgcgcggcc cgtagttgag gcgcacgagg agcacgccgt cctcgcgctc gacgatggtg
     3961 ttcgcgccga tctgcacgtg catgaagtcg gcgaagccgc agaccggaca ggtcgggcgg
     4021 tcgcgcccgt cgatgcgcgt gggcacgagc cgcgttccgt cggtcgggca aaagctaaac
     4081 gctttgccct ccctccctgc tcaaacgctc gttgtcactc gcgtgcaggt gccgctcgtc
     4141 gcgggagacc cgctcctcgc tcaactcttc gccaagcgat ccttgaggta gtcgatcgag
     4201 cggacctcgg acggatcggc gccgttcagg agcgcgagat agaaggtgac gtagtccgtg
     4261 aacatcgtca tctggagcac ttccgagagc atgttctgcc cggtgaagcg gagcgtcgtg
     4321 tggcggatgt tcgcgcgatc gagcagctcc gcggtgacgt cgagccggat cttgtgccgc
     4381 gggttgtccc ggtcgctgcg caagaggagc acggtgaggg cctcgcgcgc ggccggcggg
     4441 tgcgcgaaac cgacgacggc gttgtggttc agctcgctca tgacgtcgaa cgcggaccag
     4501 ttcttcgcgt tctcgttgaa ctgccccttc acccgccgcg ccatgacgcc gaggaccccg
     4561 gcgccgtacg cgaacgggat gcggccgtag aggtcgatcg cgagcttctt cgcgtcgttc
     4621 gtgctcgcgc cctcgtgcac gcgctcctcg agcttcgcga ggtccgcgag cgccgcctcg
     4681 atgtcggccg ccagatcgcg cacgaaaccg agccggcaga ggcagcccag cacgagcccg
     4741 agcgagtagc cgagggccgc acgcggctgc gccttgtacg cgaaggtcac cgtcgggtac
     4801 ccgccggcct tcgcgagctc cgagatgcgg ccaccggtcg tcagcacgag gatcttcgcc
     4861 ccgcgctgcc tcacggcgtc gaacgcggag agggtctcct cggtgttgcc cgaatacgag
     4921 gaggcgatca ccagcgagtc gcgaccgacg tatccgggcg cgccgtagtc gcggtgcacg
     4981 ctcatcggga tcttcagctc gtccgcgagc agcgcggccg cgaggtcgcc gccgatggcc
     5041 gagccaccca ttcccgccac ggtgatgttg cgcacgtcgg cgtaggcggg cggcagcgcg
     5101 gccgcgttcg cgatcctcca ggcgtcgcgc acctgcttcg cgaggtcctt gatgcggtcg
     5161 agcatgttgc cggggtcggc ggcgcggatc cgctccaccg atgcgatgat cgacgtgtcg
     5221 ggcgccgtgc tagccaccgg tcaccatcct ctcgcccgcg tcgagcatgg gcttcacttc
     5281 ctgttgcgcc cgcgtctcgg tgtacacgcg cacgagcggc tcggtccccg agagacgcac
     5341 caggagccag gagccgtcgt ccacgaagaa cttcacaccg tccttggtgt cgagcggcac
     5401 gaccttcttg accgcatggc ccgcgagctc gcgcggctgc gcggcgctca gccgatccat
     5461 gatctcgatc ttcttcttcg cgtaggtcgc gccctcctgg tgcaggtcgc ggcggaggta
     5521 gaacgacggg ccgaactcgg ccatcagctc gccgatgagc tgcgacggcg tcttcttcgt
     5581 ctggtgcatg aaatcgagca ggaacagatc ggcgacgatc ccgtcgcgct ccgggaggtg
     5641 catcgcgaat ccgaagccgc cggactcctc gccgcccatc atcgcgccgg tctcctgcat
     5701 cttgggaccg atgtacttga agccgacggg gacctcatgc acgggcacgc cgtacttctc
     5761 gcccaggcga tcgcacatcg aggtcatgtt cacggtcttc acgaccgggc tccgcagctt
     5821 ccggacctcg agcaggtacc acatgagcag cgcgtacacg gtgagcgtgg tgacgaagtt
     5881 gcccttctcg tcgccgaggc ccgcgcggtc ggcgtcgccg gcgacggcga ggccgacgtt
     5941 cgcgtgctcg gccgggatgc gcgcgaggaa ctcgtcgatg ttcggcggga tgggctccgg
     6001 attcacgccg ccgaagaacg ggttgcgctc gccgtgcagc tcgacgatct gggtgcgccc
     6061 gccgcccagc agacgcggga agtagccgct cgcactcccg aacagcggct cgcacacgat
     6121 cgtgtagccg gccgccctga acgcctcgag gtcgaagagc tcgccgacac gcgcgaggta
     6181 gtcgggtgcg gggtcgaaga
//
LOCUS       DASTZR010000265         3311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_640951, whole genome shotgun sequence.
ACCESSION   DASTZR010000265 DASTZR010000000
VERSION     DASTZR010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640951"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VFV20_05930"
     CDS             <1..386
                     /locus_tag="VFV20_05930"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU5287925.1"
                     /translation="YISHVAGANRPVYVISIAASLAGCHPRTLRIYEEEGLLEPVRTQ
                     TNIRLYSDDDLQRVRVIRYLTQRRGVNLAGVKMLLQFHDIADLLEELARQIEDASAAD
                     EDVDGSMDAEPRRRDGTDRYRKNGE"
     gene            398..844
                     /locus_tag="VFV20_05935"
     CDS             398..844
                     /locus_tag="VFV20_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEU5287926.1"
                     /translation="MTITRFSPMTDFVSLREAMDRLFEDSFIRPTTWTGLAAGQIAVP
                     VDLWETNEAYHLRADLPGLAPDAIDINATADTIAISGETKPVTDVATEGWLRQERRTG
                     KFQRAFTLPVQIDPDKVQASFQHGVLELVLPKADQVKPRTIKVSAK"
     gene            953..>3311
                     /gene="lon"
                     /locus_tag="VFV20_05940"
     CDS             953..>3311
                     /gene="lon"
                     /locus_tag="VFV20_05940"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEU5287927.1"
                     /translation="MKETKATEDRQYPNEVTLVPLREMVVFPKLVVPLGVGREKSVAA
                     VTAAMNEDRRLVILAAQRDAEIDDVQPDQIHNIGTIAEIVRLLRIPDGSAQIIVQGVQ
                     RVRITGYSPETRYFKVTFEPITEDLGDAVEREAQLRNVKALFDRYVDNGGAILPELAM
                     TAKNTDDPSHFADLVASSPDLSLEQRQQLLEQRSVIERLRFLAVFLAKQNEILELKQK
                     IQSEVQQTLDKTQREYILREQMKAIQKELGDDDQTSELKEIKDKIEQAGMPDPVKEKA
                     LKEVGRLEKIPQASPETGVIRTYVDWLISLPWNKSASDEWDIPNAAKILDEDHYGLPK
                     VKDRIIEYMAVRKLSQNLRAPILCFVGPPGVGKTSLGKSIARAMGRKFIRMSLGGIRD
                     EAEIRGHRRTYVGALPGRILQNMKTAGQVNPVFMLDEIDKVGADFRGDPSSALLEVLD
                     PEQNNTFTDHYLEVPYDLSKVIFITTANVEDTIIAPLRDRMEIIRLPGYTEDEKMHIA
                     KGYLVPRQLKQHGLMPADRPKEAADGETAETAVEPRPNAEEVTGETKLVIGDDVVREL
                     VRRYTREAGVRNLEREIGTICRKVARTIAEGKAEKVTVTPENLSEYLGPQRFDYGLAE
                     EEDQVGAVTGVSVSEHGGDTLTVEATVIDGKDEDFLLTGQIGKVMEESARAALSYVRS
                     HQAEFGIPKGFFEDHAMHIHVPAGAIPKDGPSAGVTMATAITSALTGRRVRRDVAMTG
                     EITLRGRVLPIGGVKEKLLAAHRAGIKTFILPEKNRKDLVDVPKEV"
BASE COUNT          658 a         1111 c         1099 g          443 t
ORIGIN      
        1 catatataag tcatgtggca ggtgcgaacc gacccgtcta cgtgatctcc atcgcggcga
       61 gcctcgccgg ctgccacccg cgcacgctgc ggatctacga ggaggagggg ctgctcgagc
      121 ccgtacggac gcagaccaac atccgcctct actccgacga cgacctccag cgtgtccggg
      181 tcatccgcta tctcacgcag cgccgagggg tcaacctcgc gggcgtgaag atgctcctgc
      241 agttccacga catcgcggat ctgctcgagg agctggcgcg gcagatcgag gatgcgagcg
      301 cggcggacga ggacgtggac gggagcatgg acgccgagcc acgccgacgc gacggcacag
      361 atcgctatcg aaagaacggg gaataggagt gagatctatg accatcacac gcttctcgcc
      421 gatgaccgac ttcgtatcgc ttcgcgaggc catggatcgt ctcttcgagg acagcttcat
      481 ccggccgacg acgtggacgg gtctcgccgc tgggcagatc gcggtcccgg tcgacctgtg
      541 ggagacgaac gaggcctatc acctgcgcgc ggacctgccc ggtctcgcgc cggatgccat
      601 cgacatcaac gcgaccgcgg acactatcgc gatctccggc gagacgaagc cggtgaccga
      661 cgtcgcgacc gagggctggc tgcggcagga acggcgcacc gggaagttcc agcgcgcgtt
      721 cacgctgccg gtccagatcg accccgacaa ggtccaggcg agcttccagc acggcgtgct
      781 cgagctcgtc ctgccgaagg ccgatcaggt gaagccccgc accatcaagg tgagcgcgaa
      841 gtagacaact cattggcaga cacgagcgcg gccggggaat gagttcctcg gccgcgccgt
      901 tcatctcacg cgacccacgt acagggaaag gacgaacaga atgagcgaag acatgaagga
      961 aacgaaggcg acggaggacc gtcagtatcc gaacgaggtc acgctcgttc cgctgcggga
     1021 gatggtcgtc ttccccaagc tggtcgtccc gctcggtgtg ggccgtgaga agtccgtcgc
     1081 cgccgtgacg gcggcgatga acgaggaccg ccgcctggtg atcctcgcgg cgcagcgcga
     1141 cgccgagatc gacgacgtgc agcccgacca gatccacaac atcggcacga tcgccgagat
     1201 cgtgcggctg ctgcgcatcc cggacggaag cgcgcagatc atcgtccagg gcgtgcagcg
     1261 cgtccgcatc accggctact cgccggagac ccgctacttc aaggtgacct tcgagccgat
     1321 caccgaggat ctcggagacg cggtcgagcg cgaggcgcag ctgcgcaacg tgaaggcgct
     1381 cttcgaccgc tacgtcgaca acggcggcgc gatcctgccc gagctcgcga tgaccgcgaa
     1441 gaacaccgac gacccgtcgc acttcgccga cctcgtcgct tcgagcccgg acctctcgct
     1501 ggagcagcgc cagcagctcc tcgagcagcg gtccgtcatc gagcggctgc ggttcctcgc
     1561 cgtgttcctc gcgaagcaga acgagatcct cgagctgaag cagaagatcc agagcgaggt
     1621 ccagcagacg ctcgacaaga cgcagcgcga gtacatcctg cgcgagcaga tgaaggcgat
     1681 ccagaaggag ctcggcgacg acgaccagac gtcggagctg aaggagatca aggacaagat
     1741 cgagcaggcc ggcatgccgg atccggtgaa ggagaaggcg ctcaaggagg tcggccgcct
     1801 cgagaagatc ccgcaggcgt cgcccgagac gggcgtcatc cgcacctacg tggactggct
     1861 catctcgctg ccgtggaaca agtcggccag cgacgagtgg gacatcccga acgcggcgaa
     1921 gatcctcgac gaggaccatt acggcctgcc caaggtcaag gaccggatca tcgagtacat
     1981 ggccgtgcgc aagctgagcc agaacctgcg cgcgccgatc ctgtgcttcg tcggaccgcc
     2041 cggcgtcggc aagacctcgc tgggcaagtc catcgcgcgc gcgatgggcc gcaagttcat
     2101 ccgcatgtcg ctcggaggca tccgcgacga ggccgagatc cgcggccacc ggcgcaccta
     2161 cgtcggcgcg ctgcccggcc gcatcctgca gaacatgaag accgcgggcc aggtgaaccc
     2221 ggtcttcatg ctggacgaga tcgacaaggt cggcgcggac ttccgcggcg acccgagctc
     2281 ggcgctgctc gaggtcctgg acccggagca gaacaacacg ttcaccgacc actacctcga
     2341 ggtgccgtac gacctgtcga aggtcatctt catcacgacg gcgaacgtcg aggacacgat
     2401 catcgccccg ctccgggacc gtatggagat catccggctg cccggctaca ccgaggacga
     2461 gaagatgcac atcgccaagg gctatctcgt gccgcgccag ctgaagcagc acgggctcat
     2521 gccggcggac aggccgaagg aagccgctga cggcgagacg gccgagaccg ccgtggagcc
     2581 gcgcccgaac gccgaagaag tcacgggcga gacgaagctc gtcatcggcg acgacgtcgt
     2641 ccgcgagctc gtgcgccgct acacgcgcga agccggcgtc cggaacctcg agcgcgagat
     2701 cggcaccatc tgccgcaagg tcgcccgcac catcgcggag ggcaaggccg agaaagtcac
     2761 cgtcacgccc gagaacctca gcgagtacct cggcccgcag cggttcgact acggcctcgc
     2821 cgaggaggag gaccaggtcg gtgcggtcac cggtgtctcg gtgtccgagc acggcggcga
     2881 cacgctcacc gtcgaggcca cggtcatcga cggcaaggac gaggacttcc tgctcaccgg
     2941 ccagatcggc aaggtcatgg aggagtctgc gcgcgccgcg ctcagctacg tgcgatcgca
     3001 ccaggccgag ttcggcatcc cgaagggctt cttcgaggac cacgcgatgc acatccacgt
     3061 tccggccggc gcgatcccga aggacggccc gtcggcgggc gtgaccatgg cgacggccat
     3121 cacctccgcg ctcaccggcc gccgggtccg ccgtgacgtc gcgatgaccg gcgagatcac
     3181 gctgcgcggc cgcgtgctcc cgatcggggg cgtgaaggag aagctcctcg ccgcccaccg
     3241 ggcgggcatc aagaccttca tcctgcccga gaagaacagg aaggatctcg tcgacgtgcc
     3301 gaaggaggtc a
//
LOCUS       DASTZR010000266         2149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_820586, whole genome shotgun sequence.
ACCESSION   DASTZR010000266 DASTZR010000000
VERSION     DASTZR010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820586"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VFV20_05945"
     CDS             <1..645
                     /locus_tag="VFV20_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEU5287928.1"
                     /translation="GAAVSTVQKDLKKLVAYSSVAHMGFVTLGIFVLNAQGGVGAVLQ
                     MLNHGFVTGALFLFVGIQYERTHRRVIADLGGLANTWPLYTAFFGVFVLASLGLPGLN
                     GFVGEFLVLVGTFQFGGAPLGGLFWGPLAAIGVILAAVYLLWMFGRVMYGPARDAYRA
                     LPDLSPIEVACAVPLLILTVALGVFPQPAIAIIEPSLDRIVKLALDPSIQVAFR"
     gene            642..2132
                     /locus_tag="VFV20_05950"
     CDS             642..2132
                     /locus_tag="VFV20_05950"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEU5287929.1"
                     /translation="MTGTDLVAIVPEILVALTAFAVVVADAALPRHASRRMLPLLVVA
                     GLVVALLAPNMWSPYPERVIFIVGAQNGAGQFFGGFVNVDEFTNFFRAVFIVLAIFAT
                     VVAPSYLERKRIPSGEYYATVLFSTLGAMTIALSTDLITLFVGLELMTIPIYVLAGIQ
                     RRDRYANEAALKYFLLGAFSSALLVYGFAWLFGVTGSTRFGDIAVALKTTGIGNGPTI
                     VALALVTVGLGFKAAVVPFHQWTPDAYDGAPTPATAFMSVGPKAAAFAAILRVLVGGM
                     GPLVVDWSTVFAVLAAVTMTVGNVVALVQTNTKRMLAYSSIAHTGYILAAVAATAAGP
                     AAGAAVLFYVFAYGLMNLGAFACLLYVDLEGTRGATLSELNGFARRHGLGALVFAVFL
                     VSLTGIPPTVGFVAKFLVIQPVLDAGYAWLAVILALNAVLAAFYYLRVVVHMYMYDPE
                     SGAPALVSPRLLSISLGVSAVAVVVLGILPNSLYQWALNAAVPIVP"
BASE COUNT          245 a          784 c          707 g          413 t
ORIGIN      
        1 ggcgcggcgg tgtcgaccgt ccagaaggat ctgaagaagc tcgtcgccta ctcgtcggtc
       61 gcgcacatgg gattcgtcac gctcgggatc ttcgtgctga acgcccaggg cggcgtgggt
      121 gcggtgctcc agatgctgaa ccacgggttc gtcaccgggg cgctgttcct cttcgtcggc
      181 atccagtacg agcgcacgca ccggcgcgtg atcgcggatc tcgggggact cgcgaacacc
      241 tggccgctct acacggcctt cttcggcgtc ttcgtgctcg cgtcgctcgg gctccccgga
      301 ctgaacgggt tcgtcggcga gttcctcgtg ctcgtcggca cgttccagtt cggcggcgcg
      361 ccgctcggcg gactcttctg gggcccgctc gcggccatcg gcgtgatcct cgcggccgtg
      421 tacctgctct ggatgttcgg ccgtgtgatg tacgggccgg cgcgtgacgc gtatcgcgcg
      481 ctgccggacc tgtccccgat cgaggtggcc tgcgcggtgc cgctcctcat cctcaccgtc
      541 gcgctcggcg tcttcccgca gcccgcgatc gccatcatcg agccgagcct cgaccgcatc
      601 gtgaagctgg cgctcgatcc cagcatccag gtggcgttcc ggtgaccggg accgacctcg
      661 tcgccatcgt gccggagatc ctggtcgcgc tcaccgcgtt cgccgtggtc gtcgccgacg
      721 ccgcgctgcc gcggcacgcg tcgcggcgga tgctgccgct cctcgtcgtc gcgggtctgg
      781 tcgtcgcgct cctcgcgccc aacatgtggt cgccgtatcc cgagcgcgtg atcttcatcg
      841 tcggcgctca gaacggcgct ggccagttct tcggcgggtt cgtgaacgtc gacgagttca
      901 cgaacttctt ccgcgccgtg ttcatcgtcc tcgccatctt cgcgaccgtg gtcgcgccgt
      961 cgtatctcga gcgcaagcgg atcccctcgg gcgagtacta cgcgacggtg ctcttctcga
     1021 cgctcggggc gatgaccatc gcgctctcca ccgacctcat cacgctcttc gtcggcctcg
     1081 agctcatgac catcccgatc tacgtgctcg ccggcatcca acgccgcgac cgctacgcga
     1141 acgaggcggc cctgaagtac ttcctgctcg gcgccttcag ctcggcgctg ctggtctacg
     1201 gcttcgcctg gctcttcggc gtcaccggct cgacgcgctt cggggacatc gccgtcgcgc
     1261 tcaagacgac cggtatcggc aacggtccga cgatcgtcgc gctcgcgctc gtcacggtgg
     1321 ggctcggctt caaggcggcg gtcgttccgt tccaccagtg gacgcccgac gcgtacgacg
     1381 gagcgccgac gccggcgacg gcgttcatgt ccgtcggccc gaaggcggcg gccttcgccg
     1441 cgatcctgcg cgtgctcgtc ggcggcatgg ggccgctcgt cgtcgactgg tcgaccgtct
     1501 tcgcggtcct cgccgcggtc accatgaccg tggggaacgt cgtcgcgctc gtgcagacga
     1561 atacgaagcg gatgctcgcg tactcgagca tcgcgcacac cggctacatc ctcgcggccg
     1621 tcgcggcgac ggcggccgga ccggcggcgg gcgccgccgt cctgttctac gtgttcgcgt
     1681 acgggctcat gaacctcggc gcgttcgcgt gcctcctcta cgtcgacctc gagggcacgc
     1741 gcggcgcgac gctgagcgag ctgaacggct tcgcgcggcg gcacgggctt ggggcgctcg
     1801 tctttgcggt gttcctcgtg tcgctcaccg gcatcccgcc gacggtcggc ttcgtcgcga
     1861 agttcctggt gatccagccg gtgctcgacg ccggctatgc gtggctcgcg gtcatcctcg
     1921 cgctcaacgc ggtcctcgcg gcgttctact acctgcgcgt cgtcgtccac atgtacatgt
     1981 acgaccccga gagcggcgcg cccgcgctcg tctcaccgcg cctgctatcg atctcgctgg
     2041 gcgtttcggc cgtcgccgtc gtcgtgctgg gcatcctgcc gaactcgctg taccagtggg
     2101 cgctgaacgc cgccgtgccg atcgttccct agcccgcgcc ggaagaccc
//
LOCUS       DASTZR010000267         3134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_954639, whole genome shotgun sequence.
ACCESSION   DASTZR010000267 DASTZR010000000
VERSION     DASTZR010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954639"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="VFV20_05955"
     CDS             complement(<1..748)
                     /locus_tag="VFV20_05955"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HEU5287930.1"
                     /translation="MKDVKEIRYQGKVHELQKDLMNAYYAELTDIAKGRSPKKNAHLL
                     IAGNPVELVRAFDMVPVFPEVNVLQIAARKASLPLIQKAEELGYAVDNCAYVKADIGL
                     WQSGYEAPYGTVPKADLLLCNYVGCNVYLNWFEHLAELTGAPAVNIDIPFIRSADGEP
                     STQDVAYVVKQLEELIAQLEQITGKSLDWKKLVRTVELSAHVGEMWSEIKSLTKLRPA
                     PYDAYFDSVTMMAVLYCLRGTPEAETFFEMA"
     gene            complement(745..1911)
                     /locus_tag="VFV20_05960"
     CDS             complement(745..1911)
                     /locus_tag="VFV20_05960"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HEU5287931.1"
                     /translation="MTLTAFAPLPVVEQYKEIVEDREFPTVREWRRRTGRAVAGSFPV
                     YSPFELAYAARMLPVGLFGGGNQIEIAHADSRFQSFICSIVKSTLELGFIDKLAPFDA
                     LLFHSICDPARNLASVMQRNFPERRSIYVHLAQNMTSAGAADYLAYEYRRVAKELGEV
                     SGHAPADDDVRASIEAFNGMRDQLRELYALRASNPEKLSTAELYTLARMATFTDPAES
                     TKLLAESLEAAMGREARPKDRIRVVVVGSFCEQPPLELIAGIEESGCYILDDDFLLGW
                     RLFKKDVPSTGDPYLALARSYLNDSLHASTKHDLRNPRAAGLIASARAHKADAVIFLG
                     AKFCEPGLFDYALYRRALEQEGIPHLFLEFEEKMWMYDKIRTEVETFVESMLFV"
     gene            complement(1908..2339)
                     /locus_tag="VFV20_05965"
     CDS             complement(1908..2339)
                     /locus_tag="VFV20_05965"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5287932.1"
                     /translation="MQTTTKGTQIRRLTKADLSRLAALSVRLGAETAEHWHERFHSNQ
                     FVVLGAEVDGNLVGYAAGEVRRSFGRTAAAGWVDAFGIDLTRRGQGVGRDLASAFLAE
                     LRSRGADHVFTLVPVHDRTLGPFFRDLGFRDESLVCLGREL"
     gene            complement(2377..>3134)
                     /locus_tag="VFV20_05970"
     CDS             complement(2377..>3134)
                     /locus_tag="VFV20_05970"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU5287933.1"
                     /translation="DSELATRIVALAGRSRRTDNGTPVFRHRGLVMDVAAHTVRVHGR
                     AIVLTAQQFAILRSLFEANGATLSRDRLLARIESLDEEPPSDRAIDLHVTRLRRRLGD
                     DARRPRWVEAVYGVGYRLATGDGATSDFAGDAEHVLSALPDPLLVIDGEHRVRFANDA
                     ATRFLALAQSEIVGQRCGEILHCRDCNGLLLEGSRCFARAVASGDAALRDVPADIAVG
                     DDRVPVRFTYARVHSDGLMTLEIRPRNEDAPLR"
BASE COUNT          486 a         1019 c         1129 g          500 t
ORIGIN      
        1 gcgccatctc gaagaaggtc tcggcctccg gcgtgccgcg caggcagtac aggacggcca
       61 tcatcgtgac cgagtcgaag tacgcgtcgt acggcgccgg gcggagcttc gtcagcgact
      121 tgatctctga ccacatctcg ccgacgtgcg ccgagagctc gacggtgcgc acgagcttct
      181 tccagtcgag gctcttgccg gtgatctgct ccagctgcgc gatgagctcc tcgagctgct
      241 tcacgacgta ggccacgtcc tgcgtggagg gctctccgtc ggccgagcgg atgaacggga
      301 tgtcgatgtt caccgccggc gctccggtga gctccgcgag gtgctcgaac caattgaggt
      361 acacgttgca gccgacgtag ttgcagagca ggaggtccgc cttcggcacc gtgccgtacg
      421 gcgcctcgta ccccgactgc cagaggccga tgtcggcctt cacgtacgcg cagttgtcga
      481 ccgcgtagcc gagctcctcg gccttctgga tgagcggcag cgacgccttg cgcgccgcga
      541 tctggaggac gttcacctcc gggaagaccg ggaccatgtc gaacgcgcgc acgagctcga
      601 ccggattgcc ggcgatgagg aggtgcgcgt tcttcttcgg cgagcgcccc ttcgcgatgt
      661 cggtgagctc cgcgtagtac gcgttcatga ggtccttctg gagctcgtgg accttgccct
      721 ggtagcggat ctcttttacg tctttcatac gaacaacatc gactccacga aggtctcgac
      781 ctctgtgcgg atcttgtcgt acatccacat cttctcttcg aactcgagga agagatgcgg
      841 gatgccttcc tgctcgagcg cccgtcggta gagcgcgtag tcgaacagcc ccggctcgca
      901 gaacttcgcg ccgaggaaga tcaccgcgtc ggccttgtgt gcgcgcgcgc tggcgatgag
      961 tcccgccgcg cgcgggttgc gcaggtcgtg tttcgtgctg gcgtgcaggc tgtcgttcag
     1021 gtacgagcgc gcgagcgcga ggtacgggtc gccggtgctc ggtacgtcct tcttgaagag
     1081 ccgccagccc agcaggaagt cgtcgtccag gatgtagcag ccggactcct cgatgcccgc
     1141 gatgagctcg agcggcggct gctcgcagaa cgagccgacc acgacgacgc ggatgcggtc
     1201 cttcgggcgc gcctcgcgac ccatcgccgc ctcgagggac tcggcgagga gcttcgtcga
     1261 ctcggccgga tccgtgaacg tcgccatgcg cgcgagcgtg tagagctcgg cggtcgagag
     1321 cttctctggg ttcgaggcgc ggagggcgta cagctcccga agctgatcgc gcatgccgtt
     1381 gaacgcctcg atcgacgcgc gcacgtcgtc gtccgccggc gcgtggccgg agacctcgcc
     1441 gagctccttc gccacacggc ggtactcgta cgcgaggtag tccgccgcgc ccgcggaggt
     1501 catgttctgc gcgaggtgga cgtagatcga tcgccgctcg gggaagttcc gctgcatcac
     1561 cgacgcgagg ttgcgcgccg ggtcgcagat tgagtggaac aggagggcgt cgaacggcgc
     1621 gagcttgtcg atgaatccga gctcgagcgt gctcttcacg atcgagcaga tgaacgactg
     1681 gaagcgcgag tccgcgtgcg cgatctcgat ctggttgccg ccgccgaaga ggccgacggg
     1741 cagcatgcgc gccgcgtacg cgagctcgaa cggcgagtac accgggaagc tgccggcgac
     1801 ggcgcggccg gtgcggcggc gccactcgcg gacggtcggg aactcgcgat cctcgacgat
     1861 ctccttgtac tgctccacca cgggcagcgg ggcgaatgcg gtcagcgtca cagttccctc
     1921 ccgagacaga cgagcgattc gtcacggaag ccgaggtcgc ggaagaaggg gcccagcgtg
     1981 cggtcgtgca cgggaacgag cgtgaagacg tggtccgcgc cgcgcgaacg gagctcggcg
     2041 aggaacgccg aggcgaggtc tcgcccgacg ccctggccgc gacgcgtcag atcgatgccg
     2101 aacgcgtcga cccatcccgc ggccgcggta cgcccgaacg accgccgcac ctcccctgcg
     2161 gcatagccga cgaggttccc gtcgacctcg gcgccgagga cgacgaactg gttggagtgg
     2221 aatcgctcat gccagtgctc cgcggtctcc gcgccgagcc gcacggagag cgcggcgaga
     2281 cgcgagaggt cggccttggt gaggcgccgg atctgtgtgc ccttggtggt ggtctgcatg
     2341 gccgcgacgc tagggacggt ctatgtccgt cctgtgtcag cggagcgggg cgtcctcgtt
     2401 gcggggacgg atctcgaggg tcatgagacc gtcgctgtgg acgcgcgcgt aggtgaagcg
     2461 caccggcacg cgatcgtccc cgaccgcgat gtcggcgggg acgtcgcgca gcgcggcgtc
     2521 gccgctcgcg acggcgcgcg cgaagcagcg ggagccttcg agcagcaagc cgttgcagtc
     2581 gcggcaatgc aggatctcgc cgcagcgctg gccgacgatc tccgattgcg cgagcgcgag
     2641 gaatcgcgtc gccgcgtcgt tggcgaagcg cacgcgatgc tcgccgtcga tgacgagcag
     2701 tggatcggga agcgcgctga gcacgtgctc ggcatcgccc gcgaagtcgg acgtcgcgcc
     2761 gtcgcccgtc gcaaggcgat acccgacgcc gtacacggcc tcgacccaac ggggccggcg
     2821 ggcgtcgtcg ccgagacggc ggcgcagccg tgtcacgtgc agatcgatgg cgcgatccga
     2881 cggcggctcc tcgtcgagcg attcgatacg cgcgaggagg cggtcgcgcg agagggtggc
     2941 gccgttcgcc tcgaacaggg agcgcaggat cgcgaactgc tgcgcggtca ggacgatcgc
     3001 gcggccgtgc acgcgcacgg tgtgcgcggc cacgtccatc acgaggccgc ggtgacggaa
     3061 gaccggtgtg ccgttgtcgg tgcggcggct gcggccggcg agcgccacga tgcgcgtggc
     3121 gagctcgctg tcat
//
LOCUS       DASTZR010000268         2137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1137525, whole genome shotgun sequence.
ACCESSION   DASTZR010000268 DASTZR010000000
VERSION     DASTZR010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137525"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VFV20_05975"
     CDS             <1..487
                     /locus_tag="VFV20_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287934.1"
                     /translation="LALLWPTYTLFPLAAVGVLVFGASGGDPVMVAIGLGLLAVNALV
                     VVNQAYFTTVAIEGDELVFRTNFGMKEERVAIGSVQRIDAKRYPGAHSGVSAPFFVVR
                     GRTSTLKVNTKPYRLASFAPLIALLRQANTRIALDAFWDRVAQGQDVSKEIALTPRSR
                     W"
     gene            complement(538..957)
                     /locus_tag="VFV20_05980"
     CDS             complement(538..957)
                     /locus_tag="VFV20_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU5287935.1"
                     /translation="MNESGLIEELLSSARVIAVVGYSKDPTRPSNSVSRYLRSMGYRI
                     IPVNPLLHGDLIDGERAFDRLADIPPDIHVDLVDVFRRGLYLDAVVDDAIAAGITAIW
                     FQLSLGNVAAAERAEAAGMRVVWDRCTAIERRRMGHV"
     gene            1025..1522
                     /locus_tag="VFV20_05985"
     CDS             1025..1522
                     /locus_tag="VFV20_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287936.1"
                     /translation="MPPRAPVIWATTGVGAERFRRRLDERHRELSVQAKLRGRTYRRS
                     RVDARSEEARRLRGEFLAALGRLSSFETASLRIMRCRYQVQLDERADDITRDYFQLWQ
                     LIARHSGEEWPLDERGAERFDFFATQLGRLEGIADALILAGRNVRLFPAPPVPWVTVD
                     AAASY"
     gene            1527..1603
                     /locus_tag="VFV20_05990"
     tRNA            1527..1603
                     /locus_tag="VFV20_05990"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1561..1563,aa:Val,seq:tac)
     gene            1654..1950
                     /locus_tag="VFV20_05995"
     CDS             1654..1950
                     /locus_tag="VFV20_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287937.1"
                     /translation="MPDEKVEVRTPNVPGYSHKVDAAKYRTMRAELLRAIPRTGDGVT
                     AADMIAAVRPRLPRGMFGHEAVQWWVKCVQLDLEARGVLLRDRTAKPLRWRRTR"
BASE COUNT          318 a          760 c          741 g          318 t
ORIGIN      
        1 tctggcgctg ctctggccga catacacgct gttcccgctc gcggcggtcg gcgtgctcgt
       61 gttcggcgcg tccgggggcg atcccgtcat ggtcgcgatc gggctcgggc tgctcgcggt
      121 gaacgccctc gtggtggtga accaggcgta cttcacgacc gtcgcgatcg aaggcgacga
      181 gctggtcttc cgcacgaact tcggcatgaa ggaggagcgc gtcgcgatcg gctcggtgca
      241 gcgcatcgac gcgaagcgct acccgggcgc gcactcgggc gtgtccgcgc ccttcttcgt
      301 cgtgcgtggc cgcacctcca cgctgaaggt gaacaccaag ccgtaccgcc tcgcctcgtt
      361 cgctccgctc atcgcgctcc tgcgtcaggc gaacacgcgg atcgcgctcg acgcgttctg
      421 ggatcgcgtc gcgcaggggc aggacgtgtc gaaggagatc gccctcacgc cgcgcagccg
      481 ctggtagccg ttcggcgcac gccacgccgg cgtcgctgcg cgcgccctga ctcgtcgcta
      541 cacgtgcccc atgcgtcggc gctcgatcgc ggtgcagcga tcccacacga cgcgcatccc
      601 cgcggcctcg gcgcgctccg ccgcggcgac gttgccgagc gacagctgga accagatcgc
      661 cgtgatgccc gcggcgatcg cgtcgtcgac gaccgcgtcg aggtagaggc cgcggcggaa
      721 gacgtcgacg aggtccacgt ggatgtcagg cgggatgtcg gcaaggcgat cgaacgcgcg
      781 ctcgccgtcg atgaggtcac cgtggaggag cgggttcacc gggatgatgc ggtaacccat
      841 cgagcgcagg tagcgcgaga cggagttgct gggtcgtgtg gggtccttgg agtagccgac
      901 gaccgcgatg acgcgggcgg atgagaggag ctcctcgatg agcccggact cattcatctt
      961 ctcacgtctc ggccgtgacg aacagccttg ttccacatca ggcgggcagg ataatcgcga
     1021 ggacatgcca ccacgcgcgc ccgtcatctg ggccaccacc ggagtcggcg ccgagcgctt
     1081 ccgcaggcgg ctcgacgaac gtcatcgcga gctgtccgtc caggcgaagc tccgcggccg
     1141 cacctaccgc cggtcgcggg tggacgcgcg cagcgaggag gcgcggcgcc ttcgcggcga
     1201 gttcctggca gcgctcggcc gcctctcctc gttcgagacg gcgagcctgc ggatcatgcg
     1261 ctgccgctac caggtgcagc tggacgagcg ggcggacgac atcacccgcg actacttcca
     1321 gctgtggcag ctcatcgcgc ggcacagcgg cgaggagtgg ccgctcgacg agcgcggcgc
     1381 ggagcgcttc gacttcttcg cgacgcagct cggccgcctc gaggggatcg cggacgccct
     1441 gatcctcgcg ggccgcaacg tgcgcctctt ccccgcgccg ccggtcccgt gggtgaccgt
     1501 cgacgccgca gcgtcatact gaatacgggc gactagctca gctggtcaga gcgtctcctt
     1561 tacacggaga aggtcctcgg ttcgagcccg aggtcgccca ccagccggcc tgctgctctc
     1621 gatacaccac gccctctcgc tatcctgcgc gacgtgcctg acgaaaaggt cgaggtccgc
     1681 acaccgaacg tccccggcta ctcccacaag gtcgacgccg cgaagtaccg cacgatgcgc
     1741 gctgagctcc tgcgtgccat cccgcgcacc ggcgatggcg tcaccgcggc ggacatgatc
     1801 gcggccgtgc gtccgcggct gccgcgcggc atgttcggcc acgaagccgt gcagtggtgg
     1861 gtgaagtgcg tgcagctcga cctcgaagcg cgcggcgtgc tcctgcgcga taggaccgcg
     1921 aagccactgc ggtggcgccg cacccgctga cgtcctgccc cggagcgcgt cggccgtgga
     1981 acacagcgca cgcgccgcgt accagggagg gtcatgccac ggggacccga ccggcccccc
     2041 tcgccagacg aaggtcctgt tccgcacaca gacctgccac aacgcggctg cctgctcgtt
     2101 aggaccaacg cggaggcctg ggcgagggcc gcccgag
//
LOCUS       DASTZR010000269         3638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1189667, whole genome shotgun sequence.
ACCESSION   DASTZR010000269 DASTZR010000000
VERSION     DASTZR010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189667"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VFV20_06000"
     CDS             <1..543
                     /locus_tag="VFV20_06000"
                     /inference="COORDINATES: protein motif:HMM:NF016439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEU5287938.1"
                     /translation="NPVYRELVGQLQQARGGNGAAPAMNDQERQHIREYVTRAKFFID
                     NINQRRQTIARITEVIVECQREFLESGIQSLRPLTRAEVGERIGMHESTVSRATAGKF
                     VLIPSGQVVAFSVFFTASLGVKDVIKQIIENEDRTRPYSDQEIVEKLRTDNGIILARR
                     TVAKYREELQILPARLRKHP"
     gene            complement(551..817)
                     /locus_tag="VFV20_06005"
     CDS             complement(551..817)
                     /locus_tag="VFV20_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287939.1"
                     /translation="MPPQKASKSQPKADASEKAKSKDREWLTTAELATKIGWREHALR
                     DAMRGKDLPGVRRTAFGYHIESSSVDEIKATLGEPPAKPNAARR"
     gene            938..3094
                     /locus_tag="VFV20_06010"
     CDS             938..3094
                     /locus_tag="VFV20_06010"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEU5287940.1"
                     /translation="MEGFLGASPLLWVVPLSGLLAVVVAAYFAWDVLRSDTGTPAMQK
                     VAGAIYTAAVAFIKRQYRTIALLALGGMVVIGLVIYFFESAPGVSATELAVRTSIAFI
                     VGAACSMASGIVGMFVAVKSNLRTAAAAQHSVADALRISLRGGAVSGILVVGLSLIGV
                     FAMFIAYGGFDHPEQAPFTIVGFGFGASFVALFAQLGGGIYTKAADLGADLVGKVEAG
                     IPEDDPRNAAVIADLVGDNVGDCAGRGADLFESTAAENIGAMILGVAIYAVTQNVAWI
                     LFPLVVRAFGLIASVIGIFTVRGSDTKSPMSSLNLGYYITVALSIAGMFLVTNQMLNE
                     KGGGIWFFYAGVVGILTSLAFVYITQYYTAGNWRPVKEIAEAAKTGPATTIISGIAVG
                     FETTAASAITIAIALFLSHFFGERWGEATGLPHGGIYGTAVATMGMLMSAAFILAEDT
                     FGPITDNAGGVASMARASEETRERTDRLDAVGNTTKALTKGYAVGSAALAAFLLFSAY
                     LDKVTDLRKTAGINPPDFHVVDLAKVDVFLGGFLGAMLVYLFSSLAIRAVGRAGGAII
                     EEVRTQFREHPGIMAGTEDPDYGRVVDITTASALRNMIAPGLLAVGAPIVVGVLLKAE
                     AMAAMLMVGTMAGVILATVLNNGGGAWDNAKKYIESGELKDENGKSIGKGTPAHAAAV
                     VGDTVGDPFKDTAGPSLHVLIKLLATITLVLAPLFV"
     gene            3290..>3638
                     /locus_tag="VFV20_06015"
     CDS             3290..>3638
                     /locus_tag="VFV20_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015386884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HEU5287941.1"
                     /translation="MKRSIDLNSDLGEGAGHDAEIMPLITSANIACGGHAGDERTMRE
                     TVELALRHRVAIGAHPGYPDRANFGRLPMAMNAMGIIESVSHQVRALAEVAAACAAAV
                     VHVKAHGAMYNQAE"
BASE COUNT          534 a         1291 c         1185 g          628 t
ORIGIN      
        1 aaccccgtct accgcgagct cgtgggccag ctgcagcagg cgcgcggcgg gaacggcgcg
       61 gcgccggcga tgaacgacca ggagcggcag cacatccgcg agtacgtcac gcgggcgaag
      121 ttcttcatcg acaacatcaa ccagcgccgg cagaccatcg cgcgcatcac cgaggtcatc
      181 gtcgaatgcc agcgcgagtt cctcgaaagc gggatccagt cgctgcgtcc gctgacgcgg
      241 gccgaggtgg gtgagcgcat cgggatgcac gagtcgaccg tgagccgcgc gaccgcgggc
      301 aagttcgtgc tcatccccag cgggcaggtc gtcgcgttct cggtcttctt caccgcctcc
      361 ctgggcgtga aggacgtcat caagcagatc atcgagaacg aggaccgcac gcggccgtac
      421 tcggatcagg agatcgtgga gaagctgcgg accgacaacg ggatcatcct cgcgcgaagg
      481 acggtcgcga agtaccgcga ggagctacag atcctgcccg cgcgtttgcg caagcacccc
      541 tagcgccggc tcagcgccgg gcggcgttcg gtttcgcggg tggctcaccg agggtggcct
      601 tgatctcgtc gaccgacgac gactcgatgt gatagccgaa cgccgtgcga cgcacgccgg
      661 gaaggtcctt cccgcgcatc gcgtcgcgga gggcgtgctc gcgccacccg atcttggtgg
      721 cgagctcggc ggtcgtcagc cattcgcggt ccttgctctt ggccttctcg gacgcgtcgg
      781 ccttgggctg gctcttcgat gctttctgtg gcggcatgca tcgaactcta gcgggtttgc
      841 cgcctcccgc gccgtcgcct aaactcggac aagagcgctg cggccccctc cgtcggggcg
      901 ggcgcttgct tcttgtcctt tcatttatcc gttttcggtg gagggcttct tgggcgcgtc
      961 accactcctt tgggtcgttc cgctttccgg actgctcgcc gtcgtcgtcg ccgcctactt
     1021 cgcctgggac gtgctccggt ctgacaccgg cacgccggcg atgcagaagg tggccggcgc
     1081 catctacacg gcggcggtcg cgttcatcaa acgccagtac cgcaccatcg cgctcctcgc
     1141 gctcggcggc atggtcgtca tcgggctcgt catctacttc ttcgagtccg caccgggcgt
     1201 ctcggcgacc gagctcgcgg tcaggacgtc gatcgcgttc atcgtcggcg ccgcgtgctc
     1261 gatggcctcg ggcatcgtcg gcatgttcgt cgcggtgaag tcgaacctgc gcaccgcggc
     1321 ggcggcgcag cacagcgtcg cggacgcgct gcgcatctcg ctccgcggtg gcgccgtctc
     1381 gggcatcctc gtcgtgggcc tctcgctgat cggcgtcttc gccatgttca tcgcctacgg
     1441 cggcttcgac catcccgagc aggcgccctt caccatcgtg ggcttcggct tcggcgcgtc
     1501 gttcgtcgcg ctgttcgcgc agctcggcgg tggtatctac accaaggccg ccgatctggg
     1561 cgccgacctc gtcggcaagg tcgaggcggg catccccgag gacgacccgc gcaacgccgc
     1621 ggtcatcgcc gacctcgtcg gcgacaacgt cggtgactgc gccggccgtg gcgccgacct
     1681 cttcgaatcc acggccgccg agaacatcgg cgcgatgatc ctcggcgtcg ccatttacgc
     1741 ggtgacccag aacgtcgcgt ggatcctgtt cccgctcgtc gtccgtgcct tcgggctcat
     1801 cgcgagcgtc atcggcatct tcacggtccg cgggagcgac acgaagagcc cgatgtcctc
     1861 gctcaacctc ggctactaca tcaccgtcgc cctctcgatc gccggcatgt tcctcgtcac
     1921 gaatcagatg ctgaacgaga agggtggcgg catctggttc ttctacgccg gcgtcgtcgg
     1981 catcctcacc agcctcgcct tcgtgtacat cacgcagtac tacacggccg gcaactggcg
     2041 cccggtcaag gagatcgcgg aggcggccaa gaccggtccg gcgaccacga tcatctcggg
     2101 catcgcggtg ggcttcgaga ccaccgccgc gtcggcgatc accatcgcca tcgcgctctt
     2161 cctctcgcac ttcttcggcg agcggtgggg tgaggcgacc ggccttccgc acggcggcat
     2221 ctacggcacc gcggtcgcga cgatgggcat gctcatgagc gccgccttca tcctcgcgga
     2281 ggacacgttc ggcccgatca ccgacaacgc cggcggcgtc gcctcgatgg cacgtgcgtc
     2341 ggaggagacg cgcgagcgca ccgaccgcct cgacgcggtc ggcaacacca cgaaggcgct
     2401 caccaaggga tacgcggtcg gctccgccgc gctcgctgcg ttcctgctct tctccgcata
     2461 tctcgacaag gtgaccgacc tgcgcaagac cgccggcatc aacccgccgg acttccatgt
     2521 cgtcgacctc gcgaaggtag acgtgttcct cggcggattc ctcggcgcga tgctcgtgta
     2581 cctcttctcg tcgctggcga tccgcgccgt cggtcgcgcc ggtggcgcga tcatcgagga
     2641 ggtccgcacc cagttccgcg agcaccccgg catcatggcc ggcaccgagg atcccgatta
     2701 cggccgcgtc gtcgacatca cgacggcctc ggcgctccgc aacatgatcg ctcccggtct
     2761 tctcgcggtc ggcgctccga tcgtggtggg cgtcctcctc aaggccgagg cgatggccgc
     2821 gatgctcatg gtcggcacca tggccggcgt gatcctcgcc accgtcctca acaacggcgg
     2881 cggcgcgtgg gacaacgcca agaagtacat cgagtctggc gagctcaagg acgagaacgg
     2941 caagagcatc ggcaagggca cgccggcgca cgccgcggcc gtcgtcggag acaccgtcgg
     3001 cgatcccttc aaggacaccg ccggcccgag cctgcacgtc ctcatcaagc tcctcgcgac
     3061 gatcacgctg gtgctcgcac cgctgttcgt ctgatcacgg attcccacga gagtcggagg
     3121 gcccgggagc gatcccgggc ccttctcttt cttttcgcat tcgcttcggc ccgcccgttc
     3181 gggcggcctg cggctcatcg cctcgccgcg gctcgcgtac ggagtcgctc ctcggggcgg
     3241 agcccctcgt cgctccctta ttgaagtcgc tcccctatag agtcggggcg tgaagcggtc
     3301 gatcgacctc aactcggacc tcggcgaggg cgcgggacac gacgcggaga tcatgccgct
     3361 catcacgtcc gccaacatcg cctgcggcgg gcacgccggc gacgagcgca cgatgcgcga
     3421 gacggtcgag ctcgcgctgc gccacagggt cgcgatcggg gcgcatcccg gctatcccga
     3481 ccgtgcgaac ttcggccggc tcccgatggc gatgaacgcg atggggatca tcgagtcggt
     3541 gtcgcatcag gtgcgcgcgc tggccgaggt cgccgccgcc tgcgcggcgg ccgtcgttca
     3601 cgtgaaggca cacggcgcga tgtacaacca ggccgagc
//
LOCUS       DASTZR010000270         2292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_377003, whole genome shotgun sequence.
ACCESSION   DASTZR010000270 DASTZR010000000
VERSION     DASTZR010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377003"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VFV20_06020"
     CDS             <1..584
                     /locus_tag="VFV20_06020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5287942.1"
                     /translation="RTNLELLERGQPVDPAERRQVLGDLVGQMERLSTLVSDLIDLAR
                     DEKTQLLVEELRLDDVVADAITEMRIRYPQIRFDLTSAPSTVRGVRSRILRAVTNLLD
                     NAGKWSPAGGVVEVAVRGPEVTVRDHGPGIAAEDEAHLFDRFWRAPSARSLPGSGLGL
                     AIVKDVAETHGGTVAFERPAGGGASFRLRFASS"
     gene            594..941
                     /locus_tag="VFV20_06025"
     CDS             594..941
                     /locus_tag="VFV20_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287943.1"
                     /translation="MRDHAARGHLVRMTTPIAPTTPSRRPLIASSFALRALATGVALS
                     TFTGMSAFAATHLLNGAAPLQPSAAAATLLTGGDDDDDARPAPTIAPARRTTIGSGIT
                     TTTTPARTRTRTS"
     gene            941..1492
                     /locus_tag="VFV20_06030"
     CDS             941..1492
                     /locus_tag="VFV20_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287944.1"
                     /translation="MEALAVSGQTVWIIARATGVASLVALAIAMLTGMAMRPKSLTWL
                     STNRAVSELHEYATTLWLPLGLAHVVALLFDPYARVGLIDLVVPFAMPYGAFAIGLGT
                     VSLQLVTVVAIAAWSRGALSRGQWLAYHRLSYLAFATAFLHGILSGTDLAYPWLAGMA
                     WFGATVLALVGTRRVLHAIPVRA"
     gene            1678..2106
                     /locus_tag="VFV20_06035"
     CDS             1678..2106
                     /locus_tag="VFV20_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287945.1"
                     /translation="MAITEVPSGAVVATLLGFLAAAIVLATVLGVPLPLVNSERRALV
                     GVVAVGFAMCVAGGWATERVVPAGPTAIVASLAGILSIVVLLAVVNGWTTVLDPVASL
                     FYGTNAIGIADRVGVLAVGVLIAVAWIASTLRQVGLVSAS"
     gene            complement(2110..>2292)
                     /locus_tag="VFV20_06040"
     CDS             complement(2110..>2292)
                     /locus_tag="VFV20_06040"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020494624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEU5287946.1"
                     /translation="AIRDSGPAYTPVPYVDGLENPGEAMRNAVRWLVKHGYTDADIAK
                     LAGGNALRVLRETWAR"
BASE COUNT          289 a          849 c          792 g          362 t
ORIGIN      
        1 tgcgcacgaa cctcgagctc ctggagcgcg gtcagccggt cgatccggcg gagcggcggc
       61 aggtgctcgg cgacctcgtg ggccagatgg agcggctctc gacgctcgtg tcagatctca
      121 tcgacctcgc ccgcgacgag aagacgcagc tcctggtcga ggagctgcgc ctggacgacg
      181 tcgtcgccga cgcgatcacc gagatgcgca tccggtatcc ccagatccgc ttcgacctca
      241 cgagcgcgcc gagcaccgtg cgcggggtcc gatcgcgcat cctgcgcgcg gtgacgaacc
      301 tgctcgacaa cgccgggaag tggagccccg ccggcggcgt cgtcgaggtc gccgtgcgcg
      361 gacccgaggt cacggtgcgc gatcacggac ccgggatcgc cgccgaggac gaggcgcatc
      421 tcttcgaccg cttctggcgc gcgccatccg cgcgctccct tcccggctcg ggtcttggcc
      481 tcgcgatcgt gaaggacgtc gccgagaccc acggcggcac cgtggcgttc gagcgtccgg
      541 ccggcggggg cgcgtcgttc aggctgcgtt tcgcgagctc ttagccgcct ctcatgcgtg
      601 accacgccgc gcgtggtcat ctggtgcgca tgacgacacc catcgcaccc acgacgccat
      661 cgcgccggcc gctcatcgcg tcatcgttcg ccctgcgagc gctcgccacc ggcgtcgcgc
      721 tgtccacgtt caccgggatg tccgccttcg cggcgacgca cctgctgaac ggcgcggcac
      781 cgctgcagcc ctcggcggcg gcggcgacgc tgctgaccgg cggcgacgac gatgacgacg
      841 cgcgtccggc gccgacgatc gctccagcgc gccgcacgac catcggctcc ggcatcacca
      901 cgacgaccac accggcccgc acgcgcacgc gtacctcctg atggaggcgc tcgccgtcag
      961 cggtcagacg gtctggatca tcgcgcgcgc gacgggcgtc gcgtcactcg ttgcgctcgc
     1021 gatcgcgatg ctgacgggca tggcgatgcg gccgaagagc ctcacctggc tctcgacgaa
     1081 ccgcgcggtc agcgagctcc atgagtacgc gaccacgctc tggctgccgc tcgggctcgc
     1141 ccacgtggtc gcgctcctgt tcgatccgta cgcacgggtc gggctcatcg acctggtcgt
     1201 gccattcgcg atgccgtacg gtgccttcgc gatcgggctc ggcaccgtga gcctgcagct
     1261 cgtgaccgtg gtcgccatcg ccgcgtggtc gcgcggcgcg ctctcgcgcg gccagtggct
     1321 cgcgtatcac cggctgtcgt atctcgcgtt cgccacggcc ttcctgcacg ggatcctgtc
     1381 ggggaccgac ctggcgtatc cctggcttgc cggtatggcc tggttcggcg cgacggtcct
     1441 cgcgctcgtg gggacgcgcc gcgtcctgca cgccataccc gtgcgtgcgt agacaccgag
     1501 cgcacgtggg gcagcgggtg taatcaggat gccgaggctc catccggcat gcctcgcgct
     1561 gtcacaccgc cacccgcgat agtgccggag ggaccaccgc gaaagcgccc gaccggcact
     1621 cgtcccgcca tcctccggga tggacgatgc gacgagagaa acggccggag ggcgtcgatg
     1681 gcgatcacgg aagtcccgag cggggcggtc gtggcgacgc tcctgggctt cctcgcggcc
     1741 gcgatcgtgc tcgccaccgt gctcggcgtg cctctgccgc tcgtcaacag cgaacgccgg
     1801 gcgctggtcg gggtcgtcgc cgtcggcttc gcgatgtgcg tcgccggcgg ctgggcgacc
     1861 gagcgcgtcg tgcccgcggg gccgacggcg atcgtcgcct cgcttgcggg catcctgtcg
     1921 atcgtcgtgc tgctcgcggt cgtgaacgga tggacgaccg tgctcgatcc ggtcgcgagc
     1981 ctcttctatg gcacgaacgc gatcggcatc gccgaccgcg tcggcgtgct cgccgtcggc
     2041 gtcctcatcg cggtcgcctg gatcgcgtcc acgctgcggc aggtcggcct cgtcagcgcg
     2101 agctgaggct cagcgggccc aggtctccct cagcacgcgc agcgcgttcc cgccggcgag
     2161 cttcgcgatg tccgcatcgg tgtacccgtg cttcaccagc caccgcaccg cgttgcgcat
     2221 cgcctcgccg ggattctcca gaccgtccac gtaaggcacc ggcgtgtacg ccgggccgct
     2281 gtcgcggatg gc
//
LOCUS       DASTZR010000271         2039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_796583, whole genome shotgun sequence.
ACCESSION   DASTZR010000271 DASTZR010000000
VERSION     DASTZR010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796583"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VFV20_06045"
     CDS             complement(<1..294)
                     /locus_tag="VFV20_06045"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287947.1"
                     /translation="MSAEFRVAGTAAKRADGVAKVTGAARFTVDLSVPGMAHAVMLRS
                     TRAHARIVRIDRDAAERAPGVLRVVTGDDCVAAGFTPYYGHVVLDHPVLAIGKV"
     gene            complement(291..767)
                     /locus_tag="VFV20_06050"
     CDS             complement(291..767)
                     /locus_tag="VFV20_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU5287948.1"
                     /translation="MPKRPIRTRLNGVERHVEIEDNVLLLEMVRGLGLVGAKLSCDMQ
                     VCGACTMLLDGRPVSACTTLAIEADGRSVETIEGLANGTALHPLQQAFVDNFAFQCGF
                     CTPGMIVAAKSLLDERPHPGAEDVRRWMEGNICRCTGYQTIVDAVLDAAQRVRSAT"
     gene            complement(769..1623)
                     /locus_tag="VFV20_06055"
     CDS             complement(769..1623)
                     /locus_tag="VFV20_06055"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEU5287949.1"
                     /translation="MLRRFEVHRVTTVDEAVALRVRFGDDAAVYAGGTELLLAMKMGV
                     ARWPHLIDVKPIPELHHISTADGILRIGATVTHWELERDSAVAQAVPAFAELERNVAN
                     IRVRAAGTLAGNIAFAEPHADPPALLMALGARVAVQGAKGTRSIPVGEFVTGMYQTGL
                     GADEIIVAVEVPIPKPGVRASYVKFQILERPSVGIAIVGDVRGGRFTGAPSVVVGAVD
                     EMPRRVDTATLAGANAREAATADALAEAARGAVDPVDDLAGSGEYKRHLVGVFARRAL
                     DALVAEAA"
     gene            complement(1635..>2039)
                     /locus_tag="VFV20_06060"
     CDS             complement(1635..>2039)
                     /locus_tag="VFV20_06060"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEU5287950.1"
                     /translation="EGLQPVADPAAERQLFELVNEERTNAGLAPLTWDERLVPIARAH
                     SAEMFKLRYFSHQSPVAGSPFDRLKAGGVTYSRAGENLAYAQSVTVAHRGLMSSEGHR
                     ENILSPQFTRIGIGVMSAGAYGRMFTQMFLTP"
BASE COUNT          293 a          738 c          716 g          292 t
ORIGIN      
        1 caccttgccg atcgcgagca ccggatggtc gaggacgacg tgtccgtagt acggagtgaa
       61 tccggccgcg acgcaatcgt cgccggtgac gaccctgagc acgcccgggg cgcgctccgc
      121 ggcgtcgcgg tcgatccgca cgatccgcgc atgggcgcgc gtggagcgca gcatcaccgc
      181 gtgcgccatg cccggcacgg agagatcgac cgtgaagcgc gccgcgccgg tcaccttcgc
      241 gacgccgtcg gcgcgcttcg ccgcggtgcc ggcgaccctg aactcggcgc tcacgtcgcg
      301 ctccgcacgc gctgggcggc atcgaggacc gcgtcgacga tggtctggta gccggtgcag
      361 cggcagatgt tgccctccat ccaccgtcgc acgtcctcgg cgcccgggtg cgggcgctcg
      421 tcgaggaggg acttcgccgc gacgatcatc cccggcgtgc agaagccgca ctggaacgcg
      481 aagttgtcga cgaacgcctg ctgcagcgga tgcaatgcgg tgccgttcgc gagcccctcg
      541 atggtctcga cgctccgtcc gtccgcctcg atcgcgaggg tggtgcacgc gctgacgggt
      601 cggccgtcga gcagcatcgt gcacgcgccg cacacctgca tgtcgcacga gagcttcgcg
      661 ccgaccaggc cgagcccgcg gaccatctcg aggagcagca cgttgtcttc gatctcgacg
      721 tgacgctcga cgccgttcag ccgcgtgcgg atcgggcgct tcggcacgct acgcggcctc
      781 cgcgacgagc gcgtcgagcg cacggcgggc gaacacgccg acgaggtggc gcttgtactc
      841 gccgcttccg gcgaggtcgt cgacggggtc cacggcgccg cgcgcggcct cggcgagcgc
      901 gtccgccgtc gccgcctcac gtgcgttcgc ccccgcgagc gtcgcggtgt cgacgcgccg
      961 cggcatctca tcgacggcgc cgaccaccac ggacggcgcg ccggtgaagc ggccaccgcg
     1021 cacatcaccg acgatggcga tgccgacgct cgggcgctcg aggatctgga acttcacata
     1081 ggacgcgcgg acccccggct tcgggatcgg cacctccacg gcgacgatga tctcgtccgc
     1141 accgaggccc gtctggtaca tgccggtcac gaattcgccg accgggatgc tgcgcgtccc
     1201 cttggcgccc tggaccgcga cgcgcgcgcc gagcgccatg agcagcgcgg gcggatcggc
     1261 atgcggctcc gcgaacgcga tgttcccggc cagcgttccg gccgcgcgga cgcggatgtt
     1321 cgcgacgttg cgctcgagct ccgcgaacgc cgggaccgcc tgcgcgactg cgctgtcgcg
     1381 ctcgagctcc cagtgcgtca cggtggctcc gatgcgcagt atgccgtcag cggtcgaaat
     1441 gtggtgcagc tccgggatcg gcttgacgtc gatcaggtgc ggccagcggg cgactcccat
     1501 cttcatcgcc agcagcagct cggtaccgcc ggcgtagacg gcggcatcgt cgccgaaccg
     1561 cacgcgcagc gcgacggcct catcgaccgt ggtgacgcgg tggacctcga agcgccgcag
     1621 catctggtct gagattaggg cgtcaggaac atctgcgtga acatgcgccc gtacgcgccc
     1681 gccgacatca cgccgatgcc gatgcgcgtg aactgcggcg acaggatgtt ctcgcggtgg
     1741 ccctcgctcg acatgagacc gcgatgcgcg accgtgacgg actgcgcgta cgcgaggttc
     1801 tcgcccgcgc gcgagtatgt cacgccgccg gccttcagcc gatcgaacgg cgaccccgcg
     1861 accggcgact gatgcgagaa atagcgcagc ttgaacatct cggcggagtg cgcgcgcgcg
     1921 atcgggacga gccgttcgtc ccacgtcagc ggcgccagtc cggcattcgt gcgctcctcg
     1981 ttcacgagct cgaacagctg gcgctcggcc gcggggtcgg cgacgggctg caggccctc
//
LOCUS       DASTZR010000272         3267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_171603, whole genome shotgun sequence.
ACCESSION   DASTZR010000272 DASTZR010000000
VERSION     DASTZR010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171603"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..1381
                     /locus_tag="VFV20_06065"
     CDS             29..1381
                     /locus_tag="VFV20_06065"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5287951.1"
                     /translation="MNRASLKLIGSIALGNGLVPLNSTMIVVALPFIARDEGIDLASA
                     SWLITLYLIVMAALHPVGGRIGDRFGRRRLMLGALLYFGLASIGAALSQGFAMLVFFR
                     LQQAIAAALVAPNGMGILKRAAGDRAGTFFGIISAVSGAAASVGPLIGGLLVGLDWRW
                     IFAVNVPICALAFALAIVNLPEEAARPNTRFDLAGVVALGLILSATAWTLTTLAHGVD
                     ATTLVLAVAVVAAGIVFIRYESGHEDAALPPALFRIRAFAAANVTIGAANMSLYATLL
                     AIPALLANDPATAVLSGVALFALGAVQIFLAPLTGMLVDRFGAKWPPAFGGLCILAGS
                     LATGALVVGGNFALLLVALMVTGSGIAFTFPASRVAAVDAVPQRYVSLASGVVQTSRY
                     LAGMIGAILVGAVLTVLPQPVRVPTLFTILAAGGAVTSLAALGMPGMTRRAPVVEEVA
                     "
     gene            complement(1378..2259)
                     /locus_tag="VFV20_06070"
     CDS             complement(1378..2259)
                     /locus_tag="VFV20_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HEU5287952.1"
                     /translation="MTATLRELLARPRILVMPGAYDALTARLVENAGFDALYLSGAGV
                     SYTHLARPDIGLVTLDEMLRRAESICETVRIPVLADGDTGYGNAINVGRTVRAFERAG
                     LSGIQLEDQSFPKRCGHLDGKEVVPAEEMAGKIRAAVDARASDDFIVVARTDAIATDG
                     FDEALRRLRLYAECGADVLFADGIATQEHLAALPGALGRPTLANMVEGGKTPLRTAAE
                     LERMGYRVVIFPNSLVRLFARQGAALLAALHESGTTNGARADMLTFDELNALIGTKAI
                     LEAGRRYAEEAPGNARD"
     gene            complement(2256..2753)
                     /locus_tag="VFV20_06075"
     CDS             complement(2256..2753)
                     /locus_tag="VFV20_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013798292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEU5287953.1"
                     /translation="MILHGRALVYGDDVSTDAIVPGKYLKLTDQEAAAHAMEGIDPTF
                     VTRVRRGDLIVGGKNFGTGSSREYAASALRIAGVSAIVAPFFARIFFRNAINQGLPVL
                     ECAEAGTIREGDELEVDVVEGIVRDVTQGTTLAATKLPPHILELIDAGGMIPWLERKL
                     AAAKR"
     gene            complement(2750..>3267)
                     /locus_tag="VFV20_06080"
     CDS             complement(2750..>3267)
                     /locus_tag="VFV20_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEU5287954.1"
                     /translation="YPDPDATYARVVRNRADELVPLVAFPGSPDNVAPATDARGERIV
                     QAYIGACTGAKYTDLAAVARVVKGRHVADGIRFLIAPASKDVTRRAAESGVLATILEA
                     GATLLPTGCGACAGLGHGILGKGERCISSTNRNYPGRMGHVEAEVFLGSPATVAASAI
                     AGTVADPRDFA"
BASE COUNT          382 a         1200 c         1166 g          519 t
ORIGIN      
        1 atgcgataga gcactagagt ttcgcttcgt gaaccgcgcc tcgctcaagc tcatcggctc
       61 gatcgcgctc ggaaacggcc tcgttccgct caattccacg atgatcgtgg tcgcgctgcc
      121 gttcatcgcg cgggacgagg gcatcgacct ggcctcggcc tcgtggctca tcacgctcta
      181 cctcatcgtg atggccgcgc tgcatccggt cggcgggcgc atcggcgacc ggttcggacg
      241 gcggcggctc atgctcggcg cgctcctgta cttcgggctc gcgtcgatcg gcgcggcgct
      301 ctcgcagggc ttcgcgatgc tcgtgttctt ccgcctgcag caggccatcg cggcggcgct
      361 cgtcgcgccc aacggcatgg gcatcctcaa gcgcgccgcg ggcgaccgcg ccggcacctt
      421 ttttggaatc atcagcgcgg tctccggcgc ggccgcgtcg gtgggcccgc tcatcggcgg
      481 cctgctcgtt ggcctcgact ggcgctggat cttcgccgtc aacgtgccca tctgcgctct
      541 cgcgttcgcg ctcgcgatcg tgaacctgcc cgaggaggcg gcgcgcccga acacgcgctt
      601 cgacctcgcg ggcgtggtgg ccctcgggct catcctctcc gccacggcgt ggacgctcac
      661 cacgctcgcg cacggcgtcg atgcgacgac gctcgtgctc gcggtcgccg tcgtcgcggc
      721 gggcatcgtc ttcatccggt acgagtccgg ccacgaggac gcggcgctgc cgccggcgct
      781 ctttcgcatc agggccttcg ccgcggcgaa cgtcacgatc ggcgccgcga acatgtcgct
      841 gtacgccacg ctgctcgcga tcccggcgct gctcgcgaac gatccggcga ccgcggtgct
      901 ctccggcgtc gcgctcttcg cgctgggcgc cgtgcagatc ttcctcgcgc cgctcaccgg
      961 gatgctcgtc gaccgcttcg gcgcgaagtg gcctccggcg ttcggcggcc tgtgcatcct
     1021 cgccggctcg ctcgcgacgg gcgcgctcgt cgtcggtggg aacttcgcgc tcctgctcgt
     1081 cgcgctcatg gtcaccgggt cgggcatcgc cttcacgttc ccggcctcgc gcgtcgccgc
     1141 ggtggacgcg gtgccgcagc gctacgtgtc gctcgcgtcg ggcgtcgtgc agacgagccg
     1201 ctacctcgcg gggatgatcg gcgcgatcct cgtcggcgcg gtgctcacgg tgctgccgca
     1261 gccggtgcgt gtcccgacgc tcttcacgat cctcgcggcg ggcggcgcgg tgacctcgct
     1321 cgcggcgctc gggatgccgg gcatgacgcg gcgcgcgccg gtcgtggaag aggtcgccta
     1381 gtcccgagcg ttccccggag cttcctcggc gtagcggcgc cccgcttcga ggatcgcctt
     1441 cgtgccgatg agcgcgttga gctcgtcgaa cgtgagcatg tcggcgcggg cgccgttcgt
     1501 cgtcccgctc tcgtgcagcg cggcgagcag cgcggcgccc tgccgcgcga agaggcgcac
     1561 caggctgttg gggaagatga cgacgcgata gcccattcgc tcgagctcgg cggccgtccg
     1621 cagcggcgtc ttcccgcctt cgaccatgtt cgcgagcgtc ggccgcccga gcgcgcccgg
     1681 gagcgctgcg aggtgctcct gcgtcgcgat gccgtcggcg aacagcacgt ccgcgccgca
     1741 ctcggcgtac aggcgcaggc gccgcagcgc ctcgtcgaag ccgtcggtgg cgatggcgtc
     1801 ggtacgcgcg accacgatga agtcgtcgga cgcgcgcgcg tccacggccg cgcggatctt
     1861 gccggccatc tcctcggccg gcacgacctc cttgccgtcg agatggccgc agcgcttcgg
     1921 gaacgactgg tcctcgagct ggatgccgct caggcccgcg cgctcgaacg cgcgcaccgt
     1981 gcggccaacg ttgatcgcgt tgccgtagcc ggtgtcgccg tcggcgagca ccgggatgcg
     2041 caccgtctcg cagatggact ccgcgcggcg cagcatctcg tccagggtca cgagcccgat
     2101 gtcgggccgc gcgagatgcg tgtacgacac gcccgcgccc gagaggtaca gcgcgtcgaa
     2161 gccggcgttc tcgacgagcc gcgcggtgag cgcgtcgtac gcgccgggca tcacgaggat
     2221 ccgcggccgc gcaagcagct cgcggagcgt cgcggtcatc gcttcgccgc ggccagcttg
     2281 cgctcgagcc acgggatcat cccgcccgcg tcgatgagct cgaggatgtg cggcgggagc
     2341 ttggtcgcgg cgagcgtcgt cccctgcgtg acgtcacgga cgatcccctc gaccacgtcc
     2401 acctcgagct cgtcgccctc gcggatcgtg cccgcctcgg cgcactcgag gaccggcagc
     2461 ccctgattga tcgcgttgcg gaagaagatg cgcgcgaaga acggcgcgac gatcgcgctc
     2521 acgccggcga tgcgcagcgc gctcgcggcg tactcgcggc tcgaccccgt cccgaagttc
     2581 ttcccgccga cgatgagatc gccccgccgc acgcgcgtca cgaaggtcgg gtcgatgccc
     2641 tccatcgcgt gcgccgcggc ctcctggtcg gtgagcttca ggtacttgcc cggcacgatc
     2701 gcgtcggtgg acacgtcgtc gccgtagacg agcgcgcggc cgtgcaggat cacgcgaagt
     2761 cccgcgggtc cgcgaccgtg cccgcgatcg cgctcgccgc gaccgtggcg ggggagccga
     2821 ggaagacctc cgcctcgacg tggcccatgc ggcccgggta gttgcggttc gtgctggaga
     2881 tgcagcgctc gcccttgccg aggatgccgt ggcccagccc ggcgcacgcg ccgcagccgg
     2941 tgggcaggag cgtggccccc gcctcgagga tggtggcgag cacgcccgat tccgccgcgc
     3001 gccgcgtgac gtccttcgac gcaggcgcga tgaggaaccg gatgccgtcc gcgacgtgcc
     3061 gccccttcac cacgcgcgcg accgcggcga ggtcggtgta cttcgcgccg gtgcacgcgc
     3121 cgatgtaggc ctggacgatc cgctcgccgc gcgcgtcggt ggcgggcgcg acgttgtcgg
     3181 gcgagcccgg gaaggcgacc agcgggacca gctcgtcggc gcggttgcgc acgacgcgcg
     3241 cgtatgtcgc atcgggatcg gggtacg
//
LOCUS       DASTZR010000273         2296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_63922, whole genome shotgun sequence.
ACCESSION   DASTZR010000273 DASTZR010000000
VERSION     DASTZR010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63922"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1456
                     /locus_tag="VFV20_06085"
     CDS             <1..1456
                     /locus_tag="VFV20_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEU5287955.1"
                     /translation="PKAGEVIDADACVIGSGAGGGVAAAVLAAAGKRVVVLEKAALVM
                     EAEFDGTELSGIRRLFVDRGLAATSDRAISVRAGSAVGGGTVVNWSTSLRAPDEVREE
                     WRRSGLDDLDPHYDAVESALDVDRDESPRNGPNAALERGLAALGLRSETIPRNTKGCG
                     DCGPCAVGCRRGAKQSTMRTYLADACRDGAEILDRTDAQRITARDGRVTGVVARVPGG
                     EITVRAPLVALAGGAILSPAVLLRSGIARETAGRSLFIHPVVAMGGIYDERLEPWRGV
                     PQSVMSDAFANLDGAYGFRIDCAPAHPGLIATGFPWWGSVSHRARIPEAAHTAPFIGI
                     VRDGAPGRVDLDRDGAARMRYFPAAAERRLLVRAMVELSRIHHAAGARRVVSLYTPPL
                     TLDDPGGLDAFTADIERRGIAPNRVVLFSAHQMGTCRVGTSPRDSVADPNGQVWGVRG
                     LFVTDASAFPSASGVNPMLSIMAIARRTAQRMLV"
     gene            1514..>2296
                     /locus_tag="VFV20_06090"
     CDS             1514..>2296
                     /locus_tag="VFV20_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019067439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEU5287956.1"
                     /translation="MTFRRDAPLDPSQVEDRRGAGGLLVGGGGIGIVILIASLLLGVD
                     PTAMLEAIPTDTAVQNGPTATECKTGSQANERDDCRIVGFVDSVQKYWSDQFARTGKQ
                     YAPAKTVLFSGYAQSACGTAQTAMGPFYCPEDGKVYMDTTFFQDLRTKLGAQAGPFAQ
                     GYVVAHEYGHHVQHLIGGLNNSTRTGAESQSVRTELQADCYAGVWAKNASETGYLETP
                     TQAEIADALDAAAAVGDDRIQQKTQGRVDPESFTHGTSAQRVE"
BASE COUNT          326 a          823 c          832 g          315 t
ORIGIN      
        1 gccgaaggcg ggtgaggtca tcgacgcgga cgcatgcgtg atcggttccg gagcgggcgg
       61 cggcgtggcg gccgccgtcc tcgcggccgc cgggaagcgc gtcgtcgtcc tcgagaaggc
      121 cgcgctcgtg atggaggcgg agttcgacgg caccgagctg tccggcatcc ggcggctctt
      181 cgtcgatcgc ggactcgccg cgacgtcgga tcgcgcgatc tcggtccgcg cgggcagtgc
      241 cgtgggcggc gggacggtcg tgaactggag cacctcgctt cgcgcccccg acgaggtgcg
      301 cgaggagtgg cgccggtccg ggctcgacga tctcgacccg cactacgacg cggtcgagtc
      361 cgcgctcgac gtcgatcgtg acgagagccc gcggaatggc ccgaacgcgg ccctcgagcg
      421 cggactcgcg gcgctcgggc tgcgctccga gacgatcccg cgcaacacca agggctgcgg
      481 cgattgcggc ccctgcgcgg tgggctgccg ccgcggcgcg aagcagtcga cgatgcggac
      541 ctacctcgcc gacgcctgtc gcgacggtgc cgagatcctc gaccgcacgg acgcgcagcg
      601 catcaccgcc agggatgggc gcgtcaccgg cgtcgtggcc cgcgtgcccg gtggcgagat
      661 caccgtgcgt gccccgctcg tcgcgctggc cggcggcgcg atcctctcgc cggcggtgct
      721 gctgcggtcg ggcatcgccc gtgagaccgc gggacgctcg ctcttcatcc accccgtcgt
      781 ggcgatgggc ggcatctacg acgagcgcct cgagccgtgg cgcggcgtgc cgcagtcggt
      841 gatgagcgac gcgttcgcga acctcgacgg cgcgtacggc ttccgcatcg attgcgcacc
      901 ggcgcacccg ggtctcatcg cgacgggctt tccctggtgg gggagcgtct cgcaccgtgc
      961 gcgcatcccc gaggccgcgc acacggcgcc gttcatcggg atcgtgcgcg acggggcgcc
     1021 cggacgcgtc gacctggacc gcgacggcgc ggcgcgcatg cgctacttcc cggccgccgc
     1081 ggagcggcgc ctcctcgtgc gcgcgatggt cgagctgtcg cgcatccatc acgcggccgg
     1141 cgcgcgccgc gtcgtcagcc tctacacgcc gccgctcacc ctcgacgatc caggcggact
     1201 cgacgcgttc accgcggaca tcgagcgccg cgggatcgct ccgaatcgcg tcgtgctctt
     1261 ctcggcgcac cagatgggca cgtgccgcgt cggcacgagc ccgcgtgact cggtggccga
     1321 tccgaacggc caggtctggg gcgtgcgcgg cctcttcgtg accgacgcga gcgcgttccc
     1381 gagcgcgtcc ggcgtgaacc ccatgctctc gatcatggcc atcgcgcgcc gcaccgcgca
     1441 gcgcatgctc gtgtgatcgc tcggttcggg cccgcacagc gccgctggcc ccggtcttgc
     1501 gtggacgacg accgtgacat tcagaagaga cgcgcccctc gatccctcgc aggtcgaaga
     1561 ccggcgcggt gccggcgggc tcctcgtggg cggcggtggc atcggcatcg tgatcctcat
     1621 cgcgtcactg ctgctgggcg tcgatcccac cgcgatgctc gaggcgatcc ccaccgacac
     1681 ggccgtccag aacgggccga ccgcgacgga gtgcaagacc ggatctcagg ccaacgagcg
     1741 agatgactgt cggatcgtcg gcttcgtcga cagcgtgcag aagtactgga gcgaccagtt
     1801 cgcgcgcacc ggcaagcagt acgcgcccgc gaagaccgtg ctcttcagcg gatacgcgca
     1861 gtccgcgtgc ggaacggcgc agaccgcgat gggccccttc tactgtccgg aggacggcaa
     1921 ggtgtacatg gacacgacct tcttccaaga tctgcgcacg aagctcggcg cgcaggcggg
     1981 tccgttcgcg cagggctacg tcgtggcgca cgagtacgga catcacgtgc agcacctcat
     2041 cggcgggctg aacaactcga cgcgcaccgg cgcggagagc caatcggtgc gcaccgagct
     2101 gcaggcggac tgctacgcgg gtgtctgggc gaagaacgcg agcgagacgg gttacctgga
     2161 gacgccgacg caggcggaga tcgccgacgc cctcgacgcc gcggccgcgg tcggcgacga
     2221 ccgcatccag cagaagacgc agggacgcgt cgaccccgag tcgttcacgc acggcacctc
     2281 ggcgcaacgc gtggag
//
LOCUS       DASTZR010000274         6841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_955412, whole genome shotgun sequence.
ACCESSION   DASTZR010000274 DASTZR010000000
VERSION     DASTZR010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6841
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955412"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(89..192)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            347..973
                     /locus_tag="VFV20_06095"
     CDS             347..973
                     /locus_tag="VFV20_06095"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HEU5287957.1"
                     /translation="MTERLVDFTLRAFSDQLASDAPVPGGGSAAAYAGALGAGLAAMV
                     ARLALKKSDDEALHDYAGEMDHLRNDFLRLVDDDSAAYGRVAEAMKLPRGTDDEKRAR
                     TERLQSALLAASRVPLEIAKTSRRLLDASERGMAKAPAAAISDIGVGALLAEAALRGA
                     AMNVMINLSSVKDPGQVKALSEDLDRALDGADAQRKRITDFVESRIAR"
     gene            973..1812
                     /locus_tag="VFV20_06100"
     CDS             973..1812
                     /locus_tag="VFV20_06100"
                     /inference="COORDINATES: protein motif:HMM:NF014881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEU5287958.1"
                     /translation="MRLLHGRPVAERVNARTKERSDALHARGVAPRLDIISVGTDPAA
                     PTYQERLEKAGRTLGIVVRRLALARTTAEPDVVERLRSSRSDPATHGVLLLTPLPAPL
                     EETRLVEELDPAKDVEGVHPYNAGLLALGRPRFVPSTAEAILELLRFHEIPIDGQHAV
                     IIGRSPVVGRPAATLLLHAHATVTLAHSRTAELAAHTRIARIVVVAVGRAGFLRGDML
                     GADTTVVDAGINVTPSGLVGDADLASVSAVAAALSPVPGGVGAVTTALLLRNVVTAAE
                     EQS"
     gene            complement(1839..2822)
                     /gene="glpX"
                     /locus_tag="VFV20_06105"
     CDS             complement(1839..2822)
                     /gene="glpX"
                     /locus_tag="VFV20_06105"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEU5287959.1"
                     /translation="MATSLDGRIPSLDLVRITEAAAIAAAHWVGHGDNRLADQAAVEA
                     MRKAMDEVAFNGTVVIGEGERDEAPMLFIGEKVGRGDGPDIDIAVDPLEGTNLVAKGR
                     ANAIAVMAVSEAGGLLHAPDTYMDKLIVGPPAKGKVDMDRPVAENLKIVADSLGRDVN
                     DLTVVILDRPRHEQLINQVRDAGARIHLIEDGDVIAALSVAVGGTGLHVVMGSGGAPE
                     GVLAAAALKCIGGEILGRLMFRNDDERARAKRMGVTDEKKVYRTDELASGKDIRFVAS
                     GVTDGELLQGVRFLARGVRTHSVILDGHVGKMRMIDTHHFEDPAHPPVVRL"
     gene            complement(2826..3983)
                     /locus_tag="VFV20_06110"
     CDS             complement(2826..3983)
                     /locus_tag="VFV20_06110"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEU5287960.1"
                     /translation="MPERTAPRVVIAPQSFKGSADAVAVAAAIARGVRRAWPSAETVE
                     LPLADGGEGTVRALVHATRGEIRKTRVHDPLLRAIDAEWGVLGDRTTAVVEMAAANGL
                     PLLRENERDPRVTSTRGTGELILAAAASGAHRIIVGIGGSATNDGGAGMARAFGYRFY
                     GRAGDELPEGGAALAQLARIDGQTDPRLVRPAIDVACDVRNPLLGPEGASAVFGPQKG
                     ATPEIVAQLDAALARYADVLERLVGRRVRDVPGAGAAGGLGAGLLAFLDARLLSGAQL
                     VLDAVGFERRLVGASLVITGEGRIDGQSAYGKLTQAVTVSARRQGIRVSAVAGMLGAG
                     HERMRDAGITHIETLATDDAGRAAAMRDPLPRIEAAAERLALQARPEMDSV"
     gene            4086..5066
                     /locus_tag="VFV20_06115"
     CDS             4086..5066
                     /locus_tag="VFV20_06115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5287961.1"
                     /translation="MTLSTSPDAGCTARVPSEIAPAWSAIAERRSPSSIFLTPEWTAV
                     ARAHSDDEAVTLAAGDPIYGIAALARAADGTIRFAGGELTDEQDVIAAPEDARAAAAA
                     IARWVAGTHPPRVLLEYVPRDTPTLDAFDAELATAGYAVRRERLVTSPRITLPGDFEA
                     YVQGLGKKERHELRRKLRRFEAAPGAGFRWADPRERPAVLDRFFALHRVSKGEKAAFM
                     TAEAESFFRDIADALAPSGRLRLGVLSAHGEDAAVLFAFAYGDTLALYNAAYDPALGS
                     LSLGIASHAFAIRDAIAQRFAVYDLLRGDEPYKYDLGASDRWLWRLEATS"
     gene            5063..6322
                     /locus_tag="VFV20_06120"
     CDS             5063..6322
                     /locus_tag="VFV20_06120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5287962.1"
                     /translation="MSRIAMLSVHACPLAKLGGRDSGGMNVYVREVARELGRRGHEVD
                     VFTRWREKDDPRIQPLGDNARVVHIQSGPFGYWPKMDVYEHLDEFAAKLEAHVAAEGK
                     RYDLIHSHYWLSAAVARDLAPRWGIPRIQMFHTLGLVKREVMDEDIDGESDVRVSIEK
                     QAVAESAATVAASQIEVGELLDLYDADRAKLHIIPCGVDPSVFRPIRQADARDMLGRD
                     QCERIILFVGRIEQIKGIDVLLRALGLLLFRRPELRNDICLLVVGGALDPEDDAPETE
                     KILELRRLVHQHRMEANVSFVGSLDQATLAKYYAAADVCAVPSLTESFGLVALEAMAC
                     GTPVVGTRVGGLQTLIEHGESGLLVPAGDYQALAESIAQVLTDHRLRTHLSHGARDRA
                     EHYTWSAVGERIEALYGKVLRDARVEV"
     gene            6325..>6841
                     /locus_tag="VFV20_06125"
     CDS             6325..>6841
                     /locus_tag="VFV20_06125"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU5287963.1"
                     /translation="MPDATFPLPKRAMAIFAHPDDVDFGCSGTLASWIERGTHVTYCV
                     ITSGQKGTHDPKMTPARMAAIREREQRAAGTAIGVEGFVFLRHQDGELERSMRLRAEV
                     CRAIRVHKPDVVFTNDPWGHYQMHPDHRVAGWSGLDGVIAARDHLFFPEQLRGGKLKH
                     HRVSRVLLFGTR"
BASE COUNT          984 a         2502 c         2386 g          969 t
ORIGIN      
        1 gctcgcgctc ttggggggat tcagctccgc cgtgacgacg aattcgccgc gccggatcag
       61 ctcgcgaaat gtactcagga cgtatgacct ctcatctttc cctcaagtca gggccggagt
      121 tggcacctct gcgggactcc gccggttgcc gaggcttcat cgggccgttc cctcagcctc
      181 tctggatgaa agggaccgcg ctggcgagag cgtaacacgt acactcgctc gcgctcatgc
      241 ccaaccgctc gttgtcactc gcgggcaggt ctcactcctc gcgcacgctc gcttcgctcg
      301 ctgaacaggg gccccttccc ctgagccgct cgtcgttcgt ctagtcatga ccgaacggct
      361 cgtcgacttc acgctgcgcg cgttcagcga tcagctcgca tcggacgccc ccgtgccggg
      421 cggcggaagc gccgcagcgt acgcgggcgc gctcggcgcc ggcctcgccg ccatggtcgc
      481 gcgcctcgcc ctgaagaaga gcgacgacga ggcgctgcac gactacgccg gcgagatgga
      541 tcacctgcgg aatgacttcc tccgcctggt cgacgacgac agcgccgcgt acggccgcgt
      601 cgccgaggcg atgaagctcc cgcgcggcac cgacgacgag aagcgcgcgc gcaccgagcg
      661 gctccaatcc gcgctgctcg ccgcgtcgcg cgtgccgctg gagatcgcga agaccagccg
      721 ccggctgctc gacgcgagcg agcgcggcat ggcgaaggcg ccggccgccg cgatctccga
      781 catcggcgtg ggcgccctcc tggccgaggc ggcgctccgc ggtgcggcga tgaacgtgat
      841 gatcaacctc tcgtccgtga aggacccggg ccaggtgaag gcgctctccg aggacctcga
      901 ccgggcgctc gacggagccg acgcgcagcg caagcggatc acggacttcg tcgaatcgcg
      961 catcgcacgc tgatgcgcct gctccacggg cggcccgtgg ccgagcgcgt caacgcgcgc
     1021 accaaggagc ggtccgatgc cctgcatgcg cgcggcgtcg cgccgcggct cgacatcatc
     1081 tcggtcggca ccgacccggc ggcgccgacg taccaggaac ggctcgagaa ggccgggcgc
     1141 acgctcggca tcgtggtgcg gaggctcgcc ctcgcgagaa cgacggccga gccggacgtc
     1201 gtcgagcgcc tgcgctcgtc gcgatccgat ccggccacgc acggggtgct gctcctcaca
     1261 ccactccccg cgccgctcga agagacgcgc ctggtcgagg agctcgaccc ggcgaaggac
     1321 gtggagggcg tacaccccta caacgcgggc ctgctcgcac tgggccggcc gcgattcgtg
     1381 ccgtcgaccg ccgaggcgat cctcgagctg ctccgcttcc atgagatacc catcgacggg
     1441 cagcacgcgg tgatcatcgg gcgcagcccg gtggtgggac ggcccgcggc gacgctgctc
     1501 cttcacgcgc acgcgacggt gacgctcgcg cacagccgta ccgcggagct cgcggcccac
     1561 acgcgcatcg cgaggatcgt cgtcgtcgcg gtcggcagag cagggttcct ccgcggcgac
     1621 atgctcggcg cggacacgac ggtggtcgac gccgggatca acgtgacgcc gtccggtctg
     1681 gtcggtgacg ccgatctggc gagcgtctcc gccgtcgcgg ccgccctcag tccggtaccg
     1741 ggcggcgtcg gcgcggtcac gaccgccctg ctgctgcgca acgtcgtcac ggccgcggag
     1801 gaacagtcct agccggcgtg cacccgcgcc gggcgcgcct agagccggac gaccggcggg
     1861 tgcgcgggat cctcgaagtg gtgcgtgtcg atcatgcgca tcttgccgac gtgcccgtcg
     1921 aggatgaccg agtgggtgcg gacgccgcgc gcgaggaagc gcacgccctg gagcagctcg
     1981 ccgtcagtga cgccggaggc gacgaagcgg atgtccttgc cgctcgcgag ctcgtccgtg
     2041 cgatacacct tcttctcgtc ggtgacgccc atgcgcttgg cccgggcgcg ctcgtcgtcg
     2101 ttgcggaaca tgagccggcc gaggatctcg ccgccgatgc acttgagcgc cgcggccgcg
     2161 agcacgccct ccggcgcccc gccgcttccc atgaccacat ggaggccggt gccgccgacg
     2221 gcgaccgaca gggcggcgat gacgtcgccg tcctcgataa ggtggatgcg cgctccggca
     2281 tcacggacct gattgatcag ctgctcgtga cgcgggcggt cgaggatgac caccgtcaga
     2341 tcgttcacgt cacgtccgag cgagtccgcc acgatcttta ggttctcggc gacgggccgg
     2401 tccatgtcga ccttgccctt cgcgggcggt ccgacgatga gcttgtccat gtacgtgtcc
     2461 ggcgcgtgga gcagtccgcc cgcctccgag acggccatga cggcgatcgc gttcgcgcgg
     2521 cccttggcga cgagattggt gccctcgagc gggtccacgg cgatgtcgat gtccgggccg
     2581 tcgccgcggc cgaccttctc cccgatgaac agcatcggtg cttcatcccg ctcgccctcg
     2641 ccgatgacga cggtgccgtt gaaggccacc tcgtccatcg ccttccgcat cgcctcgacc
     2701 gcggcctggt ccgcgagacg gttgtcgccg tggccgaccc agtgcgccgc ggcgatcgcg
     2761 gcggcttcgg tgatgcggac gagatcgagg ctggggatgc gaccgtcgag ggacgttgcc
     2821 atcgcttaca cgctatccat ttcgggtcgc gcctgcagcg cgaggcgttc ggccgccgcc
     2881 tcgatgcgcg gcagcggatc acgcatcgcc gccgcgcggc cggcgtcgtc ggtggcgagc
     2941 gtctcgatgt gggtgatacc ggcatcgcgc atgcgctcgt ggcccgcgcc gagcatgccc
     3001 gccacagcgg agacccggat gccctgacgc cgtgctgaca cggtgaccgc ctgtgtgagc
     3061 ttgccgtatg cggattgccc atcgatgcgg ccctcgccgg tgatgacgag cgacgcaccc
     3121 accagacgcc gctcgaaccc gaccgcatcg aggacgagct gggcgccgga caggagacgc
     3181 gcgtccagga acgccagcag tcccgcaccg agccccccgg ccgcgcccgc accgggcacg
     3241 tcacgcacgc gccggcccac cagacgctcg aggacgtccg cgtaccgcgc gagggcggcg
     3301 tcaagctgcg cgacgatctc gggcgtggcg cccttctgcg gaccgaacac cgcgctcgcg
     3361 ccctccggcc ccagcagcgg attgcggacg tcgcaggcga cgtcgatcgc gggacgcacc
     3421 agacgcggat cggtctgccc gtcgatgcgg gcgagctgcg cgagcgccgc gcccccctcg
     3481 gggagctcgt cgcccgcacg gccgtagaag cgatatccga acgcgcgcgc catgcccgcg
     3541 ccaccgtcgt tcgtcgcgct gccgccgatc ccgacgatga tgcgatgcgc gccgctcgcg
     3601 gccgcggcga ggatgagctc gccggtgcca cgcgttgagg tcacgcgtgg atcgcgctcg
     3661 ttttcgcgca gcaatggaag gccgttcgcg gcggccatct cgaccaccgc cgtcgtgcga
     3721 tcgccgagca caccccattc ggcgtcgatg gcccgcagca gcggatcgtg cacgcgcgtc
     3781 ttgcggatct cgccgcgcgt cgcgtgcacc aatgcgcgca cggtgccctc cccgccgtcg
     3841 gccagcggca gctcgaccgt ctcagccgac ggccatgcgc ggcgcacgcc gcgcgcgatc
     3901 gccgcggcca ccgcgaccgc gtcggcgctg cctttgaacg actgaggggc gatgacgacc
     3961 cggggcgcgg tgcgctcggg cacgacgaga acgatagcga gcgaggagtg gccctgcctc
     4021 aaacgccggg aatgtcggga cgcgacggaa cgttcagcga tggtgcccct aaactcggag
     4081 gacaggtgac cctctccacg agccccgacg cgggctgtac cgcgcgcgtc cccagcgaga
     4141 tcgctccggc gtggtccgcc atcgcggaac ggcggtcccc cagcagcatc ttcctcacgc
     4201 cggagtggac ggcggtggcg cgcgcgcatt ccgacgacga agcggtgacc ctcgcggccg
     4261 gcgatcccat ctacggcatc gcggcgctcg cgcgcgcggc cgacgggacc atccgcttcg
     4321 cggggggcga gctcaccgac gagcaggacg tcatcgccgc gccggaggac gcgcgggccg
     4381 cggcggccgc gatcgcgcgc tgggtcgccg gaacgcaccc tccgcgtgtc ctgctcgaat
     4441 acgtcccccg cgacacgccc acgctcgacg ccttcgacgc cgagctcgcc accgccggct
     4501 acgccgtgcg ccgcgagcgc ctcgtcacca gcccacgcat cacgctgccc ggcgatttcg
     4561 aggcgtacgt gcagggtctc ggcaagaagg agcgccacga gctgcgacgg aagctccggc
     4621 gcttcgaggc ggcacccgga gccggctttc gctgggcgga cccgcgggag cgccccgcgg
     4681 tgctcgaccg cttcttcgcg ctccatcggg tctcgaaggg tgagaaggcc gcgttcatga
     4741 cggccgaggc ggagtcgttc ttccgcgaca tcgcggatgc gctcgcgccg agcggccggc
     4801 tgcgactcgg cgtcctgtcg gcacacggcg aggacgccgc ggtgctgttc gcgttcgcgt
     4861 acggcgacac gctcgcgctg tacaacgcgg cgtacgatcc cgcgctcggc tccctctcgc
     4921 tcgggatcgc cagccacgcc ttcgccatcc gcgacgcgat cgcgcagagg ttcgccgtgt
     4981 acgacctcct ccgcggcgac gagccgtaca agtacgacct cggcgcgagc gatcgctggc
     5041 tctggcgcct cgaggcgacc tcgtgagccg catcgcgatg ctgtcggtgc acgcgtgccc
     5101 gctggcgaag ctcggcggac gcgactcggg cgggatgaac gtctacgtcc gcgaggtcgc
     5161 gcgcgagctc ggccggcgcg gccatgaggt cgacgtcttc acacgttggc gggagaagga
     5221 cgatccgcgc atccagccgc tcggcgacaa cgcgcgcgtg gtgcacatcc agtcgggccc
     5281 gttcggctac tggccgaaga tggacgtcta cgagcacctc gacgagttcg ccgcgaagct
     5341 cgaggcgcac gtcgccgcgg aaggcaagcg ctacgacctc atccactcgc actactggct
     5401 gtcggccgcg gtcgcgcgcg atctcgcgcc gcgctggggc atcccgcgca tccagatgtt
     5461 ccacacgctc ggcctggtga agcgcgaggt gatggacgag gacatcgacg gcgagagcga
     5521 cgtgcgcgtg tccatcgaga aacaggcggt cgcggagtcc gcggccacgg tcgccgccag
     5581 tcagatcgag gtcggcgagc tgctcgacct gtacgatgcc gaccgcgcga agctgcacat
     5641 catcccgtgc ggcgtcgacc cgagcgtctt ccggcccatc cgccaggcgg acgcgcgcga
     5701 catgctcggc cgcgaccaat gcgagcgcat catcctgttc gtcggacgca tcgagcagat
     5761 caagggcatc gacgtcctgc tgcgggcgct cggcctgctg ctcttccgcc gaccggagct
     5821 gcggaacgac atctgcctcc tcgtggtcgg cggcgccctc gacccggagg acgacgcgcc
     5881 ggaaacggag aagatcctcg agctgcggcg cctcgtgcat cagcaccgca tggaggcgaa
     5941 cgtgagcttc gtcggatcgc tcgatcaggc cacgctcgcg aagtactacg cggccgccga
     6001 cgtgtgcgcg gtgccgtcgc tgacggagtc gttcgggctc gtcgccctcg aagcgatggc
     6061 ctgcggcacg ccggtggtcg gcacgcgggt gggcggcctg cagacgctca tcgagcacgg
     6121 cgagtccggg ctgctggtgc cggccggcga ctatcaggcc ctcgccgagt ccatcgcgca
     6181 ggtactgacg gaccatcggc tccgcaccca tctgtcgcac ggcgcgcgcg accgcgccga
     6241 gcactacacc tggagcgcgg tgggcgagcg tatcgaggcc ctgtacggca aggtcctgcg
     6301 cgacgctcgc gtggaggtct agcggtgccc gacgccacgt tcccgctccc gaagcgcgcg
     6361 atggcgatct tcgcgcaccc ggacgacgtg gacttcgggt gctcggggac gctcgcctcg
     6421 tggatcgagc gcggcacgca tgtgacgtac tgcgtcatca cgagcggcca gaagggcacg
     6481 cacgacccga agatgacccc ggcgcggatg gctgcgatcc gcgaacgcga gcagcgtgcc
     6541 gccggcacgg ccatcggcgt cgagggcttc gtcttcctgc ggcaccagga cggcgagctc
     6601 gaacgttcga tgcggctccg cgccgaggtc tgccgcgcga tccgcgtgca caagccggac
     6661 gtcgtgttca cgaacgaccc gtggggccac taccagatgc atccggacca ccgcgtggcc
     6721 ggatggagcg gcctcgacgg cgtcatcgcc gcccgcgacc atctcttctt ccccgagcag
     6781 ctgcgcggcg gcaagctgaa gcatcaccgc gtgtcgcgcg tgctgctctt cggcacgcgc
     6841 g
//
LOCUS       DASTZR010000275         3217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1213334, whole genome shotgun sequence.
ACCESSION   DASTZR010000275 DASTZR010000000
VERSION     DASTZR010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213334"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..256
                     /locus_tag="VFV20_06130"
     CDS             98..256
                     /locus_tag="VFV20_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287964.1"
                     /translation="MRTAFAVPPRRTGRAEKVLAVQENVWQTPRARGAVSGDIKTAIF
                     EQVEAHDL"
     gene            256..999
                     /locus_tag="VFV20_06135"
     CDS             256..999
                     /locus_tag="VFV20_06135"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU5287965.1"
                     /translation="MGIVELVTLVVGLGVAGLAKGMSGMGLPLIATPILAGAFGPKMA
                     VTIVTIPIFVSNSLLLTQGFRRRGLLTGVVPFIVASMIGTAIGTLLLANLDAKMFAIL
                     IAAMVVVFLARGDRLLGDDPQALRARILGPLVCFVGGVLQGTTSIASPLVGSYFHAQR
                     LAPADFVFVLAAIFELNSVVQFVGYSLQGLYTAEVIAIGLIAVVPTLLALAAGIYLRG
                     KLDPMWFRRLIIVLLVVSVANLLWRTFLA"
     gene            1065..1706
                     /locus_tag="VFV20_06140"
     CDS             1065..1706
                     /locus_tag="VFV20_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5287966.1"
                     /translation="MHAIRLIGANAYLIVEDELTLVDAGHRGSLRILAPYLRRIGRDI
                     RDITRIVCTHGHPDHIGGVHEIAKASGANVHMHPADMERLRIRLREVLTGRPAPGAII
                     AFLTRAPADAVAVADGDVLPALGGLRVIHTPGHTPGSICLYSPSRRLVIVGDILQRLK
                     GQVTLPNYIFTDDMGEARRSIARLADLDIDTILFAHYPALREGARDVLRRLAS"
     gene            1706..>3217
                     /locus_tag="VFV20_06145"
     CDS             1706..>3217
                     /locus_tag="VFV20_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU5287967.1"
                     /translation="METLNDILDVSAAKFGTKDALIIKPGFRTRAWTYRDLAEVVPRV
                     ARVLAEAGIRKGDRVLLFGVNRPEYGIAILAALRAGAILVPLDVNSTAEFVAKIAERT
                     RASAALVTAQTLDRARTLGLPVHEMELLPDLARGKEPLPKPDVAADDLAEVMFTSGTT
                     GEPKGTMLTHRNLLSNAIAATEIFPIGPKQRLLSFIPLSHIFEQGPGFFCVLIAGASV
                     VYPTSRQPAVIQRTFKERRVSMILITPAAVRSLLIAIERRAQQEGKAALFAKLRRVAR
                     RLPMPLRRVLFISVHKKFGGRFRYIVSGGAALDPALGESWRELGVEVLQGYGLSETSP
                     ALTFNRLDRNRFGSVGVAVPGVEVKLAEDGEVLAKGPNVFKGYWENEEATRAALDGEW
                     FHTGDLGRFDDDGFLWLHGRKKDMIALPDGLKVYPEDVENIIAADPRIQELATPLRPT
                     LAIVVGLERAGEGLHVHAVFVEPKDPAIVAQIVRDANGRLSGSQQIRGWTIWRD"
BASE COUNT          461 a         1137 c         1107 g          512 t
ORIGIN      
        1 gacgctggcg acgggtgtgg cgccgggagc gccgccgagc acgcgccgag cgcgagcgtg
       61 accatcagga gcgcggggcg gagcacaccc tcacgacgtg cggacggcct tcgcggttcc
      121 accgcgacgg acggggcgcg ctgaaaaggt gctcgcggta caggagaatg tctggcagac
      181 gccgcgcgcc agaggcgcgg tgagcggtga catcaagacc gcgatcttcg aacaggtgga
      241 ggcgcacgac ttgtagtggg aatcgtcgag ctcgtcacgc tcgtcgtcgg tctcggcgtg
      301 gccgggctcg cgaaggggat gtcgggcatg ggtctgcccc tcatcgccac gcccatcctc
      361 gccggcgcgt tcggcccgaa gatggcggtc accatcgtga ccatcccgat cttcgtctcg
      421 aactcgctgc tgctcacgca gggcttccga aggcgcggcc tgctcaccgg cgtcgtgccg
      481 ttcatcgtcg cctcgatgat cggcacggcg atcgggacgc tgctcctcgc gaacctcgac
      541 gcgaagatgt tcgcgatcct catcgccgcg atggtcgtcg tattcctcgc gcgcggcgac
      601 cgtctgctcg gcgacgatcc gcaggcgctc cgcgcgcgga tcctcggccc gctggtgtgt
      661 ttcgtcggcg gggtgctcca gggcaccacg tccatcgcga gcccgctggt gggctcgtat
      721 ttccacgcac agcgccttgc gccggccgac ttcgtgttcg tgctcgcggc gatcttcgag
      781 ctgaacagcg tggtgcagtt cgtcggatac tcgctgcagg gtctgtacac ggcggaggtg
      841 atcgcgatcg ggctcatcgc cgtcgtcccg acgctcctcg cgctcgccgc cggcatctac
      901 cttcgcggca agctcgatcc gatgtggttc cggcgtctga tcatcgtgct gctggtcgtg
      961 tcggtcgcga acctcctctg gcgcactttc ctcgcctaga ctccccggca aggggagccg
     1021 agccgagcgc gacaaaggga agcgttgtgg aagtagctcc gggcgtgcat gcgatccgcc
     1081 tcatcggtgc gaacgcgtac ctcatcgtcg aggacgagct caccctcgtc gatgccggcc
     1141 accgcggctc gctccgcatc ctcgctccct acctccggcg gatcggccgt gacatccgcg
     1201 acatcacgcg catcgtgtgc acccacggcc atcccgacca catcggcggc gtgcacgaga
     1261 tcgcgaaggc cagcggcgcg aacgtgcaca tgcatcccgc ggacatggag cggctgcgca
     1321 tccgtctgcg cgaggtcctc acggggcggc ccgcaccggg cgcgatcatc gcgttcctca
     1381 cgcgggcacc ggccgacgcg gtcgccgtgg ccgacggtga cgttctgccc gcgctcggag
     1441 gtcttcgcgt catccacacg ccgggccaca cgcccggcag catctgcctg tactcgccct
     1501 cgcggcgcct cgtgatcgtc ggggacatcc tgcagcggct caaggggcag gtcacgctcc
     1561 cgaactacat cttcaccgat gacatgggcg aggcacgacg ctcgatcgcg cgccttgcgg
     1621 acctggacat cgacaccatc ctcttcgccc actaccccgc cctgcgcgag ggcgcgcgtg
     1681 acgtgctgcg taggcttgct tcgtgatgga gacgctgaac gacatcctgg acgtgtccgc
     1741 ggcgaagttc ggcacgaagg acgcgctcat catcaagccg ggcttccgca cgcgcgcgtg
     1801 gacctatcgc gatctcgcgg aggtcgtccc gcgcgtggcc cgcgtgctcg ccgaggccgg
     1861 catccgcaag ggagaccggg tgctgctctt cggggtgaac cggcccgagt acgggatcgc
     1921 gatcctcgcg gcgctgcgcg ccggcgcgat cctcgtgccg ctcgacgtga actcgaccgc
     1981 cgagttcgtc gcgaagatcg cggagcgcac ccgcgcgtcg gcggcgctcg tcacggcaca
     2041 gacgctcgac cgggcgcgga cgctcgggct cccggtgcac gagatggagc tgctgccgga
     2101 cctcgcgcgc ggcaaggagc cgctgcccaa gcccgacgtc gcggccgacg acctcgccga
     2161 ggtcatgttc acctcgggca cgaccggcga gccgaagggg acgatgctca cgcaccgcaa
     2221 cctgctgtcg aacgcgatcg cggcgacgga gatcttcccc atcgggccga agcagcgcct
     2281 gctctccttc atcccgctct cgcacatctt cgagcagggc ccggggttct tctgcgtcct
     2341 catcgcgggc gcgtccgtcg tgtatccgac gagccggcag cccgccgtga tccagcgcac
     2401 gttcaaagag cgccgcgtct cgatgatcct catcaccccc gccgcggtgc ggtcgctgct
     2461 gatcgcgatc gagcggcgcg cgcagcagga gggcaaggcg gccctcttcg cgaagctgcg
     2521 ccgcgtcgcg aggcggctgc cgatgccgct gcggcgcgtg ctcttcatct cggtgcacaa
     2581 gaagttcggc ggtcggttcc ggtacatcgt ctcgggcggc gccgcgctcg atcccgcgct
     2641 cggcgagtcg tggcgcgagc tcggcgtcga ggtgctccag ggctacgggc tgtccgagac
     2701 gtcgcccgcg ctcaccttca accggctgga ccgcaaccgc ttcggctcgg tcggcgtcgc
     2761 ggtccccggc gtcgaggtga agctcgccga ggacggcgag gtcctcgcga agggaccgaa
     2821 cgtcttcaag ggctattggg agaacgagga ggcgacgcgc gcggcgctcg acggcgagtg
     2881 gttccacacc ggcgacctcg gccgcttcga cgacgacggg ttcctctggc tccacggccg
     2941 gaagaaggac atgatcgccc tgcccgacgg gctcaaggtc tatcccgagg acgtcgagaa
     3001 catcatcgcc gcggatccgc ggatccagga gctggcgacg cccctccggc cgacgctggc
     3061 gatcgtcgtg ggtctggaac gggccggcga agggctccac gtgcacgcgg tgttcgtcga
     3121 gccgaaggat ccggcgatcg tcgcgcagat cgtgcgcgac gcgaacgggc ggctgtccgg
     3181 gagccagcag atccgcggct ggaccatctg gcgggac
//
LOCUS       DASTZR010000276         3744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1060028, whole genome shotgun sequence.
ACCESSION   DASTZR010000276 DASTZR010000000
VERSION     DASTZR010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060028"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VFV20_06150"
     CDS             complement(<1..491)
                     /locus_tag="VFV20_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287968.1"
                     /translation="MGKSISRQRVIQLLDDPDFHLLAAEYPDMYADEIDAMNDYVRSV
                     TPAIPDYVLANAPTALKFVGTRVKRIEDPARITGQALYASDIKLPDMLQVAVVRSPHA
                     HALIESIDTSAAEALPGVRAVLTYKNAPKTLLGTNPDKLVVNQEAHFAGEEVAAVAAD
                     DLHT"
     gene            complement(504..1133)
                     /locus_tag="VFV20_06155"
     CDS             complement(504..1133)
                     /locus_tag="VFV20_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU5287969.1"
                     /translation="MAKQVQLTRRTLLKGAGVTGAAAAIPVTAAVRQNAPFARPAAVH
                     IGPPAGAAATTVMQVTVNTVLFEVALDPRSTLAEFLRDQLNLTGTKLGCNRGECGACT
                     VLVDGKAVVSCSMLAVDAAGKKITTVESLAGNGKLNPLQQAFWEQGATQCGYCSPGML
                     MSATALLNTKPKPTEDDVRRALSGNLCRCTGYKKIVEAVLVASGQPPKA"
     gene            complement(1464..1901)
                     /locus_tag="VFV20_06160"
     CDS             complement(1464..1901)
                     /locus_tag="VFV20_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287970.1"
                     /translation="MELDPAAAGRALDFLGVVANSLVTLHADFLLGFIVGVAGLRRYR
                     RGGWADVGYASFLGGLTAVLLLFIVFVGLRLDVPAWLLFLTGLLAAGLAWLASGALAL
                     RVIARRVRAAAARILAGAALRARPPQIPDRLPQELRSLDAHRR"
     gene            complement(1998..2666)
                     /locus_tag="VFV20_06165"
     CDS             complement(1998..2666)
                     /locus_tag="VFV20_06165"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEU5287971.1"
                     /translation="MEAQNEICPDLLRAINACAEPVFTVDETWTITHWNKSAETAFGQ
                     NATDVQGRRCYEVIAGIDDGGREVCRIHCEKWALARRGARIQNFDVRAMPTHDKWANV
                     SILPISDASGRTFALAHLLRNVGRTKRLEHFIQEIATNAEDVLAPHASNGVAHGPAPI
                     HLTNRELEVLMLLSHGAGTNTIAEKLGVSRHTVHNHIAVVLNKLGVHSRAEAVAYAFE
                     HHLA"
     gene            2746..3510
                     /gene="yqeC"
                     /locus_tag="VFV20_06170"
     CDS             2746..3510
                     /gene="yqeC"
                     /locus_tag="VFV20_06170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HEU5287972.1"
                     /translation="MTTLSEALGIGDREIVAFVGAGGKTVAMFHLGRELRAAGARPVL
                     TTTTKILVPAESPDQDVVVESDPAALIAAVERALARGRIPVAAVATTADGKLAGVRPE
                     RVDDLAAAAGATHVLVEADGAARKPFKAPNDDEPVIPRATTLVVAVVGIDALGRPVSE
                     AAHRPERVTALTGLGPADVLDARSIARVMLAERGVMKGAPAGARTVVLVNKADDAARL
                     RDARSLARELMAQGAQRVVVAALERGDAIIEVSRRS"
     gene            complement(3507..>3744)
                     /locus_tag="VFV20_06175"
     CDS             complement(3507..>3744)
                     /locus_tag="VFV20_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdenum hydroxylase"
                     /protein_id="HEU5287973.1"
                     /translation="VGYVGDDPVRSTFAGYLRGIIQSGVRVRAGMKIGDVDPRAAREH
                     CFTISDKALAIGGGVLEAVLYLFERHSLRTLAAI"
BASE COUNT          548 a         1245 c         1363 g          588 t
ORIGIN      
        1 gtgtgcaggt cgtcggccgc caccgccgcg acctcctcgc ccgcgaagtg cgcctcctgg
       61 ttcaccacga gcttgtcggg gttggtgccg aggagggtct ttggcgcgtt cttgtacgtg
      121 agcaccgcgc gcacgccggg aagggcctcg gcggccgacg tgtcgatgct ctcgatgagc
      181 gcgtgcgcat gcggtgagcg aacgacggcg acctggagca tgtcgggcag cttgatgtcg
      241 gacgcgtaga gggcctggcc ggtgatgcgc gccgggtcct cgatccgctt cacgcgcgtg
      301 ccgacgaact tcagcgcggt cggcgcattg gcgagcacgt agtcggggat cgccggcgtg
      361 accgagcgca cgtagtcgtt catcgcgtcg atctcgtccg cgtacatgtc cgggtattcc
      421 gccgcgagca ggtggaagtc ggggtcgtcg aggagctgaa tgacgcgctg gcgagagatg
      481 ctcttaccca tgtgatcccc tccctatgcc ttcggtggct gaccggaggc gacgaggacc
      541 gcctcgacga tcttcttgta gcccgtgcag cgacagagat tcccggagag cgcgcggcgc
      601 acgtcatcct cggtcggctt tggcttcgtg ttgagaaggg cggtggcgct catgagcatg
      661 ccggggctgc agtagccgca ctgcgtcgcg ccctgctccc agaacgcttg ctgcagcggg
      721 ttgagcttgc cgttgccggc gaggctctcg accgtcgtga tcttcttccc cgcagcgtct
      781 accgcgagca tcgagcagga cacgaccgcc ttgccgtcga cgaggacggt gcaggcgccg
      841 cattcgccgc ggttgcagcc gagcttcgtc ccggtgaggt tgagctgatc cctcaggaac
      901 tcggcgagcg tggagcgtgg gtcgagagcg acctcgaaga gcacggtgtt gaccgtcacc
      961 tgcatcaccg tggtcgcggc cgcgcccgcg ggcgggccga tgtggacggc cgccggtctc
     1021 gcgaacggag cgttctgccg gacggcggcg gtcaccggta tcgcggcggc ggcaccggtc
     1081 acgccggcac ctttcaacag ggtcctgcgc gtgagttgga cttgctttgc cacgctgccc
     1141 ctccccgccc gatggcccgt ggggcctcgg acgtcatgcg aatccggtgg tcatcgtggc
     1201 ccagcgcacc aggcagcaca tggcacgcgc gtactgatta cctgatacgg actcaccggg
     1261 cgcttggccg cgctctctcg tcggtctcag cggcgtttcg tgctcgtgat gagggtctcg
     1321 gcatcgctcg cgagacctgt cgaggtcatg cgcgacgcat cgactagata gatcgtgtca
     1381 ggcgcgaccc agcccgagcg tgcccggtgc gggatacccg tggaaaagcc gaccaacgcg
     1441 cgcgcgaggc gccgtcgttc gtgtcagcgg cggtgggcgt cgagcgagcg gagctcctgc
     1501 gggagacgat cggggatctg cggcggtcgc gcgcgcaggg ccgcaccggc gaggatgcgc
     1561 gccgcggccg cgcggacgcg ccgcgcgatg acgcgtagag cgagcgcgcc cgacgcgagc
     1621 cacgcgagac cggccgcgag caggccggtg agaaagagca gccatgccgg cacgtccaga
     1681 cgcagcccga cgaagacgat gaaaaggagg aggacggcgg tcaggcctcc gaggaagctc
     1741 gcgtacccga cgtcggccca tccgccacgc cggtagcgcc gaaggcccgc gacgccgacg
     1801 atgaagccca gcaggaagtc ggcgtggagc gtgacgaggc tgttcgccac gacgccgagg
     1861 aagtcgagcg cgcgtccggc ggccgccggg tccagctcca cgatcacgca tcttcggacg
     1921 tcccgcgccc gcgcacacgg cacgtccgtc cctgattggt gatacgcgcg ggtcagcgca
     1981 aggcgcggat cgggcggcta ggcgaggtgg tgttcgaagg cgtacgcgac ggcttcggcg
     2041 cggctgtgca cgccgagctt gttgaggacg acggcgatgt ggttgtgtac cgtgtggcgg
     2101 ctcacgccga gcttctccgc gatcgtgttc gtgcccgccc catgagacag gagcatgagc
     2161 acctcgagct ctcgattcgt gagatggatg ggcgccgggc cgtgcgcgac gccgttgctc
     2221 gcgtgcggag cgagcacatc ctcggcgttc gtggcgatct cctggatgaa gtgctcgagt
     2281 cgcttggtcc ggccgacgtt gcgcagcagg tgcgccaggg cgaacgtgcg tcccgatgcg
     2341 tccgagatcg gaaggatcga cacgttggcc cacttgtcgt gcgtcggcat cgcgcgcacg
     2401 tcgaagttct ggatgcgcgc accgcgtcgc gcgagcgccc acttctcgca gtggatacgg
     2461 cagacctcgc ggccgccgtc atcgatgccc gcgatgacct cgtagcaccg gcgaccctgt
     2521 acgtccgtcg cgttctgtcc gaatgccgtc tccgcgctct tgttccagtg cgtgatggtc
     2581 caggtctcgt ccacggtgaa gacgggctcc gcgcaggcgt tgatcgcacg cagcagatcc
     2641 gggcagatct cgttctgcgc ttccatgggc atattcacct acaccgctgg gtagggggcc
     2701 gtagggcaaa cgcctacgtc cttgtgggac ttacgctcag tccccgtgac gacgctcagc
     2761 gaggcgctcg gcataggcga tcgcgagatc gtcgccttcg tgggagcggg cggcaagacc
     2821 gtcgcgatgt tccatttggg ccgcgagctg cgtgccgcgg gcgcccggcc ggtcctcacc
     2881 acgacgacga agatcctcgt gccggcggag agcccggacc aggacgtcgt cgtcgagagc
     2941 gaccccgcgg cgttgatcgc cgccgttgag cgcgcgctgg cgcgtggcag gatccccgtc
     3001 gccgcggtcg cgacgacggc ggacggcaag ctggcgggcg tacggcccga gcgggtggat
     3061 gatctcgccg ctgcggccgg ggcgacacat gtgctcgtcg aagccgacgg cgccgcgcgg
     3121 aagccgttca aagcgccgaa cgacgacgag ccggtgatcc cgcgagccac gacgctcgtc
     3181 gtcgccgtcg tcgggatcga cgcgctcggc cgacccgtgt cggaggccgc gcatcggccc
     3241 gaacgcgtca cggcactcac cgggctcgga cccgcggacg tgctcgacgc gcgctcgatc
     3301 gcccgcgtga tgctggccga gcgcggcgtg atgaaaggag cgcccgcggg tgcccgcacc
     3361 gtcgtcctcg tgaacaaggc cgacgacgcg gcgcgcctgc gcgatgcgcg ttcgctcgcg
     3421 cgcgagctca tggcgcaggg tgcccagcgc gtcgtcgtcg cggcgctcga gcgcggcgac
     3481 gccatcatcg aggtgagccg gcgctcctag atcgcggcga gcgtgcggag cgagtgtctc
     3541 tcgaagaggt agaggaccgc ctcgagcacg ccgccgccga tcgccagcgc cttgtccgag
     3601 atggtgaagc agtgctcgcg cgccgcacgc ggatcgacgt cgccgatctt catgccggcg
     3661 cgcacgcgga cgccgctctg gatgatcccg cggagatagc cggcgaacgt gctgcgcacg
     3721 ggatcgtcgc cgacgtaacc gacg
//
LOCUS       DASTZR010000277         2014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1085953, whole genome shotgun sequence.
ACCESSION   DASTZR010000277 DASTZR010000000
VERSION     DASTZR010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085953"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1681)
                     /locus_tag="VFV20_06180"
     CDS             complement(<1..1681)
                     /locus_tag="VFV20_06180"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU5287974.1"
                     /translation="MTDDQGREEPPGLEERVTRPGTRPGDRRVRIVRPSAAEFRRRGG
                     HYVATTDALSSRNRGGRLYESVRRFLLGERLTSEQEAGERLSKKTGLAIMASDNISSS
                     AYATEEAMRVLALAGAGALVLTMPIALAVCVVLAVVILSESRVIRAYPNGGGSYVVAK
                     DNHGVIPGLIAASALLIDYVLTVAVSSAAGVAAISSFIHPVADHRVLWGLALIALLTL
                     GNLRGIREAGVLFAAPTYVYVVSLFGLIVVGLVRVLGGDTPAPVTPHESFPVEGMEAL
                     TALVVLRAFASGSVGLTGSEAVANGVPNFKPPEPRNAATTLVIMGSLFAILFLGLTYL
                     ATTIGIRPDRSEIETVNSMLTRSIVGDGTPFYYVVQIATAVILLLAANTGFTGFPRLA
                     SVLADDRFMPRQFAFRGERLAFSFGIIALALMSALVLVAFGGSVTNLVPLYTIGVFVA
                     FTLSQSGLVRRWRRLRNPGWRTSMVINSFGAVVTFVVLIVVAYTKFTYGAWMVLVVMP
                     ILVALLYGINRHYTSVADALTLASPDEPLPVVKPPVVIVPVARLDRATLQAV"
     gene            complement(1835..>2014)
                     /locus_tag="VFV20_06185"
     CDS             complement(1835..>2014)
                     /locus_tag="VFV20_06185"
                     /inference="COORDINATES: protein motif:HMM:NF025701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HEU5287975.1"
                     /translation="GDRLVIRATFTVPAPGGIVTQVLTSPAHVVAIASGDMAGVRTVV
                     LLDQSGAERARTTLS"
BASE COUNT          352 a          676 c          720 g          266 t
ORIGIN      
        1 ccaccgcctg cagcgtcgcg cgatcgagcc gggccaccgg gacgatcacg accggcggct
       61 tcacgacggg cagtggctcg tcgggcgagg ccagcgtcag cgcgtcggcc accgaggtgt
      121 aatgccggtt gatgccgtac agcagcgcga cgaggatcgg catgacgacg aggaccatcc
      181 aggccccgta cgtgaatttc gtgtacgcga cgacgatgag gacgacgaac gtcacgaccg
      241 cgccgaagga gttgatcacc atcgacgtcc gccaccccgg gttccgcagg cggcgccagc
      301 ggcgcacgag tccgctctgc gacagcgtga acgcgacgaa gacgccgatg gtgtagagcg
      361 gcacgaggtt cgtgacgctg ccgccaaagg cgacgagcac gagcgccgac atcagcgcga
      421 gcgcgatgat cccgaacgag aaggccaggc gctcgccgcg gaacgcgaac tgacgaggca
      481 tgaagcggtc gtccgcgagc accgacgcga gccgtgggaa cccggtgaac ccggtgttcg
      541 cggccagcag caggatgacc gcggtcgcga tctgcacgac gtagtagaag ggcgtcccgt
      601 cgccgacgat cgagcgcgtg agcatgctgt tcaccgtctc gatctcgctt ctgtctggcc
      661 tgatgccgat cgtcgtggcg agataggtca ggccgaggaa gaggatggcg aacagcgacc
      721 ccatgatcac gagcgtggtc gcggcgttgc gcggctccgg cggtttgaag ttcggcacgc
      781 cgttcgcgac cgcttcgctc cccgtcagcc ccaccgaccc cgacgcgaac gccctcagca
      841 cgacgagcgc cgtcagggcc tccattccct cgaccgggaa ggactcgtgt ggtgtgacgg
      901 gtgcgggcgt gtcaccaccg agcacgcgga cgaggccgac gacgatgagg ccgaacagcg
      961 acacgacgta cacgtaggtc ggcgcggcga agaggacgcc ggcctcgcgg atgccgcgga
     1021 gattcccgag ggtgagcagc gcgatgagcg cgaggcccca gagcacgcga tggtcggcga
     1081 cggggtggat gaacgacgag atcgccgcga cgcccgccgc gctcgagacg gcgaccgtga
     1141 ggacgtagtc gatgagcagt gccgatgccg cgatgagacc gggaatgacg ccgtggttgt
     1201 ccttcgccac gacgtacgaa ccgccgccat tcggatacgc gcgaatgacg cgcgactcgg
     1261 acaggatgac cacggcgagg acgacgcaca ccgcgagcgc gatgggcatc gtgagcacga
     1321 gcgcgccggc accggcaagc gcgagcacgc gcatcgcctc ctcggtcgcg tacgccgagg
     1381 acgagatgtt gtccgacgcc atgatcgcga gtcccgtctt cttcgagagg cgctcgcccg
     1441 cctcctgctc cgacgtgaga cgttcgccga gaaggaaccg ccgaacgctc tcatagagcc
     1501 gcccgccgcg gttacgcgac gagagcgcgt cggtcgtggc cacgtagtgg ccgccgcggc
     1561 gacggaactc cgcggcggac ggccggacga tgcgcacacg acgatcaccg ggccgcgtgc
     1621 ccggacgcgt gacgcgctcc tcgagtcccg gcggctcctc cctgccctgg tcgtccgtca
     1681 tcgtcggact cggcgtgcgc gaagtggcac gggacgcacg aggttgccca tcaccacgat
     1741 gatgggcctt ccgggctcgc ggcggtcacg agcgtcgtta cggtgccgcg tctggccgtc
     1801 cagggcggcg tgtgcggcga gattccgatc tcgttcagga cagcgtcgtc cgcgctcgct
     1861 cggcaccact ttggtcgagc aggacgacgg tgcgcacgcc tgccatgtca ccgctcgcga
     1921 tcgcgacgac gtgcgccggt gaggtgagca cctgcgtcac gatgccgccg ggcgccggga
     1981 cggtgaacgt cgcgcggatc acgagccggt cgcc
//
LOCUS       DASTZR010000278         3915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_37943, whole genome shotgun sequence.
ACCESSION   DASTZR010000278 DASTZR010000000
VERSION     DASTZR010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37943"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..927)
                     /locus_tag="VFV20_06190"
     CDS             complement(70..927)
                     /locus_tag="VFV20_06190"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HEU5287976.1"
                     /translation="MPKRGASSVWISIDMEGIGGVATYSHVMMQGVEYERARRWMTDE
                     ANACIDGAANAGARRFCVNDSHGAMHNIFADDLDPRAELLVGTAKPWSMGQGIDGDYD
                     TCFFVGYHARAGHPKGVLDHTYASRAIYELRLDGRPVGETTLNAYLAGYFGSTVSLVA
                     GDDATCREARQLLGREVVAVRTKDAAGRFAAKMIHPSKVCEALRDGAARAVSGAASLK
                     PLVPRGKSEVEIAFLTSAMADMAELIPGTRRAGARTVAYAARDYLELYKAMLAMVRIA
                     PSAVPPEQS"
     gene            987..1069
                     /locus_tag="VFV20_06195"
     tRNA            987..1069
                     /locus_tag="VFV20_06195"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1020..1022,aa:Leu,seq:cag)
     gene            complement(1244..2881)
                     /locus_tag="VFV20_06200"
     CDS             complement(1244..2881)
                     /locus_tag="VFV20_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5287977.1"
                     /translation="MTARDKFIVVALVALMAVISVGAVFLDQAERTGVVPAYGGTHIE
                     GVVGQPQYLQPILAATDVDQDVSRLVFNGLTQLGRDGSVVADLATFTTEGDGKIWTFT
                     IRSDAKWQDGQLVTAADVVYTVSLVQDKAYVGPYSDAFRGVKTEAVSERVVRFTLPEA
                     FGSFAANTTLPLLPAHRLSGVTYNDLPRQQFNLRPIGSGPFRVVEVDARQVVLSRNDD
                     YYKVHPDRVRPYLDRIILRSYPDASAALTALSRGEIDGAGGLSTSDAERARTYKNVNL
                     YSFPTSDYTALFFNVRPEKAVFRDRVVRQAIATAIDKGRVLDVAADGRGRVADELVPP
                     TSWAYVKDVTRYAHSIETARQMLDAADWKDHDNDGVRDKGGVKLAFGISTSDEPARVA
                     AALQIVDDLRQIGIVGELRAQPFSSLVESTVPRRSYDALLIGITGTGDPDPYPLFHSS
                     EIADPGHNFSGYFTLPLDRALENSRRTSDQAKRLELIAPVFQAVSAEVPVVFLYFSDY
                     LYAQSKQVQGLRIMPVTSPSDRLWNVQDWYVKTEVQR"
     gene            complement(2882..3109)
                     /gene="secG"
                     /locus_tag="VFV20_06205"
     CDS             complement(2882..3109)
                     /gene="secG"
                     /locus_tag="VFV20_06205"
                     /inference="COORDINATES: protein motif:HMM:NF015779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU5287978.1"
                     /translation="MDLLQVAQIVIAVAVSASILLQARGTGLSSTFGGESTAYRSRRG
                     LERTLFRLTVVLIVVYVAISLFSARGGGPTA"
     gene            complement(3132..>3915)
                     /locus_tag="VFV20_06210"
     CDS             complement(3132..>3915)
                     /locus_tag="VFV20_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287979.1"
                     /translation="AALTSAPDKLVRVHVVDWNALPWRAELAAPLVLLGLIALAVPIA
                     TIIVTRPSTTIVTAYAVGAVGVMLLGGREGATINYLLDLSAAVALAVAGRAPLLACGR
                     AYPAAALVQALLVVLLFDPLGVVPGRIVQTGAWGDPSRIDAVREIPGTLLVEDSGVLL
                     ATGREPVVDDVFLWSRNRAREVAREMSFLEGERLLGTVRAGTFDAVVSEVDLARVAQI
                     GGFEAQRWHPDLVAAVLERYTLARHAMGLGRYGDGLYVYVRR"
BASE COUNT          608 a         1311 c         1400 g          596 t
ORIGIN      
        1 cccgatccgc ctccgctcac tgatggagcg gcgaaagccg ctccagagag caagagccaa
       61 cggcgagggt catgactgct cgggcggcac cgccgacggc gcgatccgca ccatcgccag
      121 catcgccttg tagagctcga gatagtcgcg cgcggcgtac gccacggtgc gcgcgcccgc
      181 gcgccgcgtg cccgggatga gctcggccat gtccgccatg gcggaggtga ggaacgcgat
      241 ctccacctcg ctcttgcccc tcggcacgag cggcttgagc gacgcggcgc ccgagaccgc
      301 gcgcgccgcg ccatcgcgca gcgcctcgca gaccttggag ggatggatca tcttcgccgc
      361 gaagcgaccc gccgcgtcct ttgtccgcac ggcgacgacc tcgcgaccga ggagctgccg
      421 cgcctcgcgg cacgtcgcgt cgtcgccggc gaccagcgac acggtcgatc cgaaataccc
      481 ggcgagatac gcgttcagcg tcgtctcgcc gaccggtctt ccgtcgaggc gcagctcgta
      541 gatcgcgcgg ctcgcgtagg tgtggtcgag cacgcccttc gggtggccgg cgcgcgcgtg
      601 atacccgacg aagaagcagg tgtcgtagtc gccgtcgatg ccctgcccca tgctccacgg
      661 cttcgccgtg ccgacgagca gctccgcgcg cggatcgagg tcatcggcga agatgttgtg
      721 catcgcgccg tgcgagtcat tcacgcagaa gcggcgagcg ccggcgttcg cggctccgtc
      781 gatgcaggcg ttcgcttcgt cggtcatcca tcggcgggcg cgctcgtatt cgacgccctg
      841 catcatcacg tggctgtagg tggcgactcc gccgatgcct tccatgtcga tcgagatcca
      901 tacgctggag gcaccgcgct tcggcacgtg gccgatggta agaggtcgca tcggccgacg
      961 cgtttcggat agaatggtga tgtccagcgg gagtggcgga acggcagacg cgataggttc
     1021 agggcctatt gctcgaaagg gcgtgagggt tcaaatccct ccttccgcac tgcgtcggat
     1081 cttcgtacgt cacctaccca gatgtcccgc gttggtccgt ggcggcggtg aggcccttcg
     1141 cccagtgcgt gtcgatccgt gcctctcgct caagctcgcg gagcgttgtg tggccgaatt
     1201 gcgtgacatc acacgccgcg agatgtctca tcctcgtcga tgcctagcgt tggacctcgg
     1261 tcttcacgta ccagtcctgg acgttccaga gccggtcgct cggtgaggtg accggcatga
     1321 tccgcaggcc ttgcacctgt ttgctctgcg cgtagaggta gtcggagaag tacaggaaca
     1381 ccaccgggac ctcggcagag accgcctgga acaccggcgc gatgagctcg aggcgcttcg
     1441 cctgatcgga cgtgcggcgc gagttttcga gcgcgcggtc gagcggcagc gtgaagtagc
     1501 cggagaagtt gtggcccggg tcggcgatct cgctcgagtg gaacagcggg tacggatccg
     1561 ggtcgccggt cccggtgatg ccgatgagca gcgcgtcgta cgagcgccgc ggcacggtgc
     1621 tctcgacgag gctgctgaag ggctgcgcgc gcagctcgcc gacgatgccg atctgccgca
     1681 ggtcatccac gatctgcagc gccgcggcga cgcgggcggg ctcgtccgag gtgctgatgc
     1741 cgaacgcgag cttcacgccg cccttgtcac gcacgccgtc gttgtcgtgg tccttccagt
     1801 cggccgcgtc cagcatctgc cgcgccgtct cgatggagtg cgcgtagcgc gtcacgtcct
     1861 tcacgtaggc ccaggacgtc ggcggcacga gctcgtcggc gacgcggcca cggccgtccg
     1921 ctgcgacgtc gaggacccgc cccttgtcga tggcggtggc gatcgcctgt cgcacgaccc
     1981 ggtcgcggaa gacggccttc tcagggcgaa cgttgaagaa gagcgccgtg tagtcggacg
     2041 tcgggaagct gtacaggttc acgttcttgt aggtgcgcgc gcgctcggcg tcgctcgtcg
     2101 agagcccgcc ggcaccatcg atctcgccgc gactcagcgc ggtgagcgcg gccgacgcat
     2161 cgggatacga gcgcaggatg atgcggtcca ggtagggccg cacccggtcc ggatgcacct
     2221 tgtagtagtc gtcgttccgc gacagcacga cctggcgcgc gtcgacctcg acgacgcgga
     2281 acggtccgct gccgatcggg cggaggttga actgctggcg cggcaggtcg ttgtaggtga
     2341 cgccgctgag ccggtgtgcc ggaagcagcg gcagggtcgt gttcgcggcg aacgagccga
     2401 acgcctcggg cagtgtgaag cggacgaccc gctcggacac cgcctccgtc ttcacgccgc
     2461 ggaaggcgtc gctgtacgga ccgacgtagg ccttgtcctg gacgagcgag accgtgtaga
     2521 cgacgtcggc cgccgtgacg agttggccgt cctgccactt cgcatcgctg cggatcgtga
     2581 aggtccagat cttcccgtcg ccttcggtgg tgaacgtcgc gaggtcggcg acgaccgagc
     2641 cgtcgcggcc gagctgcgtg agcccgttga acacgagccg tgacacgtcc tgatcgacat
     2701 cggtcgcggc gaggatcggc tggaggtact ggggctgccc gacgacgcct tcgatgtggg
     2761 tgccgccgta cgcgggcacg acgccggtgc gttcggcctg atcgaggaac acggcgccga
     2821 cgctgatcac cgccatgagc gccacgagtg cgacgacgat gaatttgtcg cgcgccgtca
     2881 cctaggcggt cgggcccccg cctcgcgcac tgaagagcga gatcgcgacg tacacgacga
     2941 tgaggacgac cgtcaggcga aagagcgtgc gctccagccc gcgccggctg cggtacgcgg
     3001 tcgactcgcc accgaaggtg gacgagaggc cggtcccgcg cgcctgcagc aggatcgagg
     3061 cgctgacggc cacggcgatg acgatctggg cgacctgaag gagatccatg cggtcattcg
     3121 attgtacggg gtcagcggcg gacgtacacg tacagcccgt cgccgtagcg gccgaggccc
     3181 atcgcgtgcc gcgcgagcgt gtagcgctcg aggaccgcgg ccacgaggtc ggggtgccag
     3241 cgctgcgcct cgaagccgcc gatctgcgcg acgcgggcga gatcgacctc gctcacgacg
     3301 gcgtcgaagg tgccggcacg cacggtgccg aggaggcgct cgccctcgag gaacgacatc
     3361 tcgcgtgcga cctcgcgcgc gcggttgcgc gaccagagga acacgtcatc gaccacgggt
     3421 tcgcgtccgg tcgcgaggag cacgccgctg tcctcgacga gcagggtgcc ggggatctca
     3481 cgcaccgcgt cgatgcgtga gggatcaccc cacgcgccgg tctggacgat gcgtcccggg
     3541 acgaccccga gcggatcgaa cagcagcacc acgaggagcg cctggaccag cgcggccgcc
     3601 ggataggcgc gcccgcacgc aaggaggggc gcgcgaccgg cgaccgccag cgcgacggcg
     3661 gccgacaggt cgaggaggta gttgatcgtc gcgccctcgc gcccgccgag gagcatgacg
     3721 ccgaccgcgc cgacggcgta cgcggtcacg atcgtcgtgc tcgggcgcgt gacgatgatg
     3781 gtagcgatcg gcacggcgag cgcgatcagc ccgagcagca ccagcggcgc ggcgagctcg
     3841 gctcgccacg gcagcgcgtt ccagtcgacc acgtgcacgc gcacgagctt gtccggcgcg
     3901 ctcgtgagcg ccgcg
//
LOCUS       DASTZR010000279         5031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_977635, whole genome shotgun sequence.
ACCESSION   DASTZR010000279 DASTZR010000000
VERSION     DASTZR010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977635"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VFV20_06215"
     CDS             <1..431
                     /locus_tag="VFV20_06215"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5287980.1"
                     /translation="LVSTLSVFASWETFGRTTTIDRFTTDESRARIAKRKEGAYAAIA
                     AAKRAGVRIAAGSDFGGGSVRAGHLAWEVELLVAAGLEPFEALAAATRNGGALYGVDH
                     AGRIEVGMPADLVLVHGDPLSDPRAMWRVWAVYQAGVRVA"
     gene            454..1335
                     /locus_tag="VFV20_06220"
     CDS             454..1335
                     /locus_tag="VFV20_06220"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU5287981.1"
                     /translation="MYVAVALCWSGSWTFGKLGVALVPPIELSAIRFGLAGALMLAIA
                     LAVRAPLGLERWPLLVLSGGFGIFAYNALVFVGLTMAPASDGALIVPTINPVLTVLFA
                     AFIGERVTANKIAGLAIATVGAVLVIAAATGLTFTGQRLAGDLLMLGGAASWSAYATL
                     GAITTRRGSPLGVTAVASLAGAAMLFPLGFLEKGYADVPSWPASAWLDIAYLVVFATT
                     VGFVLFYWAVRRFGAGMASMVSYLVPIFALVQAVLILGEQPAPLEIVGGAIILAGVRV
                     ATLRFRPASPIEEAEPA"
     gene            1376..3337
                     /locus_tag="VFV20_06225"
     CDS             1376..3337
                     /locus_tag="VFV20_06225"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEU5287982.1"
                     /translation="MDSTVERTIERDFMDEAVRPCDDFFQYANGSWLAKTKIPDDQPA
                     WGGFAELHERNQNTLHEILEEAARSSSDDPNVRQVGDLYASGMDEDAIASAGLAGVAG
                     LLDRIDRVRDARELAPVIAELHTLRVGAPFAMGIWPDAFDSSRQLIHFGQGGLGLPDR
                     ENYLRTDPKSVELQEAYRAHVAATLTLLGAAADRADADARAIYDLEHALAEASMPRAE
                     QRDPYKISNPFTRAKLDAHAPSIDWSAFFAGIGADVADLNVRQPAFFTALDALVRERP
                     LELWRVYLRWHLARGLAPYLPRPFERSSFEFYSRTLQGQPEEKPRWKRVLAVVDQQLP
                     HPLGRLYVDRAFPPHAKQRILDLVADLRAVLGERIATLEWMGEDTRRAARVKLDAFGV
                     KMGYPDTWRDFGALRIDRGAYVDNVMRAVRFNFAYDIGKLGRPVDKGEWLMSPPTVNA
                     YYNPPRNEIVFPAGILQPPFFFAEGDAAVNYGAIGAVIGHEMTHGFDDAGSQYDAEGN
                     LRNWWQPADRQAYDSRTDLVVRQYEEFEPLTGMRINGKLCLGENIADLGGLKIAYAAF
                     ERYLAKHGEPAPIDGFTARQRFFLGYAQAWRSLMRDELLRVRLTVDPHSPPRFRVLGP
                     LANLPEFFAAFGCDGGAMARAPELRPTIW"
     gene            complement(3365..3757)
                     /locus_tag="VFV20_06230"
     CDS             complement(3365..3757)
                     /locus_tag="VFV20_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012948266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5287983.1"
                     /translation="MAQAKVIKVKAEAKQNETFRTVLFTAEKSQLVMMSLRPGEDIGE
                     EVHADVDQLLYAVKGEGVAVADGKELAFDKGTVFCVPAGVKHNVKNTGDEPMKLFTVY
                     APPQHAPGTVHETKADAAKAEKAETPVA"
     gene            3916..>5031
                     /locus_tag="VFV20_06235"
     CDS             3916..>5031
                     /locus_tag="VFV20_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEU5287984.1"
                     /translation="MNAAAGTPLLFYADGYKFPDVYHLTRFLSPDPIIALEDGDEIVV
                     VANALEEGRVRKQSRATSVFNIDAFGAKELSAKGVGREELDANVIARFLASRGAKRVT
                     VAAYFPLGMAERLRGLGIELVVANELGERRRAKRDDEVAALEQTQRATEDAWARGVEA
                     LRRATVRTDRTLELDGEVFTAERLRAIVEGRLLELGCVSEGAIIAPGPQAADPHMIGS
                     GPLHASEPIVMDIFPQHKTTRYFADMTRTVSKGRPPDEILKMYEVTRHAQDEGIKALR
                     PGVTGRAVHELVEDIIFAAGYDTLRPGQQHTPQDGGLPRGFIHGTGHGVGLEIHEAPS
                     VGRGGTKPLAVGDVVTVEPGVYDPEIGGVRLEDMLVIT"
BASE COUNT          695 a         1806 c         1752 g          778 t
ORIGIN      
        1 cgctggtctc cacgctctcg gtcttcgcgt cgtgggagac ctttgggcgc acgacgacga
       61 tcgaccggtt cactaccgac gaatcgcgcg cccgcatcgc gaagcgcaag gagggcgcat
      121 acgccgcgat cgccgcggcg aagcgcgccg gcgtgcgcat cgccgctggg tccgatttcg
      181 gcggcggctc ggtccgcgcc ggccacctcg cgtgggaggt ggagctgctc gtcgccgcgg
      241 gcctcgagcc gttcgaggcg ctcgccgcgg cgacgcggaa cggcggggcg ctctacggcg
      301 tcgaccacgc cggccgcatc gaggtcggga tgcccgcgga tctggtgctc gtgcacggcg
      361 acccgctgtc ggatccgcgc gcgatgtggc gcgtgtgggc ggtgtatcag gcgggggtgc
      421 gcgtcgccta gatctcgtct gctcgtcgtc gccgtgtacg tcgccgtcgc cctctgctgg
      481 agcggcagct ggacgttcgg caagctcggt gtcgcgctcg tgccgccgat cgagctgtcg
      541 gcgatccgct tcggcctcgc gggcgcgctc atgctcgcga tcgcgctcgc cgtgcgcgcg
      601 ccgctcgggc tcgagcgctg gccgctcctc gtgctctcgg gcggattcgg gatcttcgcg
      661 tacaacgcgc tcgtcttcgt cgggctcacc atggcgcccg cgtcggacgg cgcgctcatc
      721 gtgccgacca tcaaccccgt gctcacggtc ctcttcgcgg cgttcatcgg cgagcgcgtg
      781 accgcgaaca agatcgccgg cctcgccatc gcgacggtcg gcgccgtgct ggtcatcgcc
      841 gcggctaccg ggctcacgtt caccggccag cgcctcgcgg gcgatctgct catgctcggc
      901 ggggccgcgt cgtggagcgc gtacgcgacg ctcggcgcga tcacgacgcg gcgcggctcg
      961 ccgctcggcg tcaccgccgt ggcgtcgctc gcgggcgccg cgatgctctt cccgctcggc
     1021 ttcctggaga agggctacgc ggacgtgccg tcctggcccg cgagcgcatg gctcgacatc
     1081 gcctacctcg tggtgttcgc gacgacggtc gggttcgtac tcttctactg ggcggtgcgg
     1141 cgcttcgggg ccggcatggc ctcgatggtc agctacctcg tgccgatctt cgcgctcgtg
     1201 caggcggtgc tcatccttgg cgagcaacct gcaccgctcg agatcgtggg cggggccatc
     1261 atcctcgcgg gcgtgcgggt ggcgacgctc cggttccggc ccgcgtcgcc gatcgaggaa
     1321 gccgagccgg cctagcccga gcgtggtatc catctcacac acaggggagg cgcaggtgga
     1381 cagcacagtg gagcggacga tcgagcgcga cttcatggac gaggccgtgc ggccgtgcga
     1441 cgacttcttc cagtacgcga acggctcctg gctcgcgaag acgaagatcc cggacgacca
     1501 gccggcctgg ggcgggttcg ccgagctgca cgagcggaac cagaacacgc tgcacgagat
     1561 cctcgaggag gcggcgcggt cgtcgtcgga cgacccgaac gtgcggcagg tcggcgatct
     1621 ctacgcgagc gggatggacg aggacgccat cgcgtccgcg ggcctcgccg gcgtcgcggg
     1681 cctccttgat cgcatcgatc gcgtccgtga cgcgcgcgag ctcgcgccgg tcatcgccga
     1741 actgcacacg ctgcgcgtgg gcgcgccgtt cgcgatgggg atctggccgg acgcgttcga
     1801 cagctcgcgg cagctcatcc actttggaca gggcgggctc ggcctccccg accgcgagaa
     1861 ctacctgcgc accgacccga agtccgtcga gctccaggag gcctaccgcg cgcacgtcgc
     1921 ggcgacgctg accctgctcg gcgcggccgc cgatcgcgct gacgcggacg cgcgcgccat
     1981 ttacgacctc gagcacgcgc tcgccgaggc gtcgatgccg cgcgccgagc agcgcgatcc
     2041 gtacaagatc agcaatccgt tcacgcgcgc gaagctcgac gcccacgcgc cgtcgatcga
     2101 ctggtccgcc ttcttcgccg gcatcggggc cgacgtcgcc gacctcaacg tgcgccagcc
     2161 cgccttcttc accgccctcg acgcgctggt ccgcgagcgt ccactcgagc tgtggcgcgt
     2221 gtaccttcgc tggcatctcg cgcgcggcct cgcgccctat ctaccgcgcc cgttcgaacg
     2281 ctcgtcattc gagttctaca gccggacgct gcaggggcag cccgaggaga agccgcgctg
     2341 gaagcgcgtc ctcgcggtgg tcgaccagca gctgccgcac ccgctcggcc ggctctacgt
     2401 ggaccgcgcg ttcccgccgc acgcgaagca gcgcatcctc gacctcgtcg ccgacctgcg
     2461 cgccgtgctc ggcgagcgca tcgccacgct cgagtggatg ggggaggaca cgcgccgtgc
     2521 cgcgcgcgtg aagctcgacg cgttcggcgt gaagatgggc taccccgaca cgtggcgcga
     2581 cttcggcgcg ctccggatcg accgcggcgc gtacgtcgac aacgtcatgc gcgctgtccg
     2641 cttcaacttc gcgtacgaca tcggcaagct cggcaggccg gtcgacaagg gcgagtggct
     2701 catgagcccg cccacggtga acgcgtacta caacccgccg cgcaacgaga tcgtgttccc
     2761 ggccgggatc ctccagccgc cgttcttctt cgccgaaggc gacgccgcgg tgaactacgg
     2821 cgcgatcggc gcggtcatcg gacacgagat gacgcacggc ttcgacgacg ccggcagcca
     2881 gtacgacgcc gagggcaacc tgcgcaactg gtggcagccc gcggaccgcc aggcctacga
     2941 ctcgcgcacg gacctcgtcg tccgccagta cgaggagttc gagcccctca ccggcatgcg
     3001 catcaacggc aagctctgtc tcggcgagaa catcgccgac ctcggcggcc tcaagatcgc
     3061 gtatgccgcg ttcgagcggt atctcgcgaa gcacggcgag cccgcgccca tcgacgggtt
     3121 caccgcccgg cagcggttct tcctcggata cgcccaggcc tggcgcagcc tgatgcgcga
     3181 cgagctgctc cgcgtgcgcc tcaccgtcga cccgcactcg ccgccgcgct tccgcgtcct
     3241 cggcccgctc gcgaacctgc cggagttctt cgcggcgttc gggtgcgacg gcggcgcgat
     3301 ggcgcgcgcg ccggagctgc gaccgacgat ctggtagcgg gcttcgtcgc tcgcgcgcgg
     3361 tgttctaggc gacgggggtc tcggccttct cggctttggc cgcgtcggcc ttcgtctcgt
     3421 ggacggtgcc gggcgcgtgc tgcggcgggg catacacggt gaagagcttc atcggctcgt
     3481 cgccggtgtt cttcacgttg tgctttacgc cggccggcac gcagaagacc gtgcccttgt
     3541 cgaatgccag ctctttcccg tccgccaccg ccacgccctc gcccttgacc gcgtagagca
     3601 gctggtcgac gtccgcgtgg acctcttcgc cgatgtcctc gccggggcgc agcgacatca
     3661 tcacgagctg gctcttctcg gccgtgaaga ggaccgttcg gaacgtctcg ttctgcttcg
     3721 cctcggcctt gaccttgatg accttcgcct gtgccatcgc acacctccgc ccgtgtcaga
     3781 ttcggaccac tcagcctcgg tgaccgcagc gtctccggat gtcaggcagc ggcgaacagc
     3841 gacgcggggc gtccggagcg ggccatttcg caccatcgcg aaagaatccc tagatttggc
     3901 ggactgggaa gacctgtgaa cgccgccgcg gggacaccac tcctcttcta cgccgatggg
     3961 tacaaattcc cggacgtcta ccacctcacg cggttcctct cgcccgatcc gatcatcgcg
     4021 ctcgaggacg gcgatgagat cgtcgtcgtc gcgaacgcgc tcgaagaggg gcgcgtgcgc
     4081 aagcagtcgc gcgcgacgag cgtcttcaac atcgacgcat tcggcgcgaa ggagctatcg
     4141 gcgaaggggg tcggccgcga agagctcgac gcgaacgtga tcgcgcgctt cctcgcctcg
     4201 cgcggggcga agcgcgtgac ggtcgccgcg tacttcccgc tcggcatggc cgagcgcctg
     4261 cgcggtctcg gcatcgagct cgtggtggcg aacgagctcg gcgagcggcg gcgcgcgaag
     4321 cgcgacgacg aggtggccgc gctcgagcag acgcagcggg cgacggagga tgcctgggcg
     4381 cgcggcgtcg aggcgttgcg gcgcgcgacg gtccgcaccg accgcacgct cgagctcgac
     4441 ggcgaggtgt tcaccgccga gcggctgcgg gcgatcgtcg agggccggct cctcgagctc
     4501 ggctgcgtgt cggaaggcgc gatcatcgcg ccgggcccgc aggccgcgga cccgcacatg
     4561 atcggcagtg gtccgctgca cgcgagcgag ccgatcgtca tggacatctt cccgcagcac
     4621 aagacgacgc ggtacttcgc ggacatgaca cgcacggtct ccaagggccg gccgccggac
     4681 gagatcctca agatgtacga ggtcaccagg cacgcacagg atgagggcat caaggcgctt
     4741 cgtcccggcg tcacggggcg cgcggtgcac gagctggtcg aggacatcat cttcgccgcc
     4801 ggctacgaca cgctgcgccc cggccagcag cacacaccgc aggatggcgg cctgccgcgc
     4861 ggcttcatcc acggcaccgg ccacggggtg ggtctcgaga tccacgaggc gccgagcgtg
     4921 ggccgcggcg gtacgaagcc gctcgcggtg ggcgacgtcg tcaccgtcga gcccggtgtg
     4981 tacgacccgg agatcggcgg ggtgcgcctc gaagacatgc tcgtcatcac c
//
LOCUS       DASTZR010000280         3177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_485848, whole genome shotgun sequence.
ACCESSION   DASTZR010000280 DASTZR010000000
VERSION     DASTZR010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485848"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VFV20_06240"
     CDS             <1..306
                     /locus_tag="VFV20_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287985.1"
                     /translation="ASPVETAPASIPVPSPQRPTELVIRFREALSYERSVEIFKRISE
                     VLADFSGQAAVVLELPRPTGKPTRVSTPFHANASEDVAKAVEREVGADVVEVVLPPR"
     gene            357..1934
                     /locus_tag="VFV20_06245"
     CDS             357..1934
                     /locus_tag="VFV20_06245"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5287986.1"
                     /translation="MPERARRLHRASAELLTSLDLEQVEREVVSALRDILAADAAAVM
                     LLDGDDKVSIRAYEGLSAEYVHGQRLPIDRMRAAYHTPDEHLIVDLRHAPLGDPALIA
                     SEGLARALVIPLTKDGQIIGILNAYMKDPKARFAEDSIDLAHVLAAEASVAIANAQLY
                     KEALDGREFERALVESLGTPVFVARTPAMVAGLNRAAADLTGIDPVPGADAQTLLSSL
                     GARLVETGEALTQSPLDIGLAGREWRGEVAFSHLVTGEERVADVIVHPVRDPEGKVIA
                     AVATLHDLTELRRVEEEKEQFLSIVSHELRTPLTPLKALAQLLIARMRRSRERGTPLD
                     LDSFERNLASIERQVDRMNGLVSDLLSVSRAGRGQLEMDRRPFDLATSVRDIVERYVE
                     VTREEGRHRFAVDAPPTLVVTADQARVEQMLMNLVGNAVKYSPRGGDVRVSLEQRDGV
                     AQITITDEGIGISSEDLPRLGTAAFTRGTGKAATFAGMGIGLHVAKLLADAHGGSLEL
                     ESEGEDRGTTVRVKLPA"
     gene            complement(1938..2022)
                     /locus_tag="VFV20_06250"
     tRNA            complement(1938..2022)
                     /locus_tag="VFV20_06250"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1985..1987),aa:Leu,seq:caa)
     gene            complement(2035..2832)
                     /locus_tag="VFV20_06255"
     CDS             complement(2035..2832)
                     /locus_tag="VFV20_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEU5287987.1"
                     /translation="MSVLSGADARRYARLARLAARTATQVGDAIVTSDIGIVTRKRGR
                     ANFATAADHAAEGAIIQRLAEHDPAIPVLAEESAKRSVRSAERLWVVDPIDGTLNFSR
                     GLPFYCVVIAYVEDGRTRAAAVHAPRTRETFVASEGAGAALNGERIAISRVARLADAF
                     AVASLRYGETKRPESRFVALNATCARLRVFGSAALEIAYVAMGRFDLFAHEALSPWDI
                     AAPALIAREAGAAVLSLKTGKDAAWDEGRVVIANKTLARAAFEMLRR"
     gene            complement(2820..>3177)
                     /locus_tag="VFV20_06260"
     CDS             complement(2820..>3177)
                     /locus_tag="VFV20_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5287988.1"
                     /translation="VVIGEDDEPIARLLRDAINDEPAYQAVIVHDGALVLETVRQVHA
                     DLLILDVMMPGLNGLEVWDRVRADPDVRNMPVLFVTATSKDMGHEFKRRKINDVIAKP
                     FDLNDLLAKVRALCPS"
BASE COUNT          496 a         1109 c         1099 g          473 t
ORIGIN      
        1 gcctcgccgg tcgagaccgc accggcatcg ataccggtgc cctcaccaca gcgtccgacc
       61 gagctggtca tccgcttccg tgaggcgctc agctacgaac ggtccgtgga gatcttcaag
      121 cgcatctcgg aggtgctcgc ggacttctcc ggtcaggcgg ccgtcgtcct cgagctgccg
      181 cgccccaccg gcaagccgac gcgggtgtcg acgccgttcc acgcgaacgc gagcgaggac
      241 gtcgccaagg ccgtcgagcg cgaggtcggt gcggacgtgg tcgaggtcgt gctcccgccg
      301 cgctaacacg ctcggtcgct cgccgcactc ccggtcggcg cgctcatact cgggacatgc
      361 ccgagcgcgc gcgacgtctc catcgagcga gtgccgagct cctcaccagc ctggatctcg
      421 agcaggtaga gcgcgaggtc gtatcggcgc tgcgcgacat cctcgccgcc gacgcggcgg
      481 ccgtgatgct gctcgacggg gacgacaagg tgtcgatccg cgcgtacgaa ggcctctccg
      541 cggagtacgt gcacgggcag cgcctcccca tcgatcgcat gcgtgcggcc tatcacacac
      601 ccgacgaaca tctcatcgtc gatctgcgtc acgccccgct cggcgatccc gcgctcatcg
      661 cctccgaagg ccttgcgagg gcgctcgtga tcccgctcac gaaggacggc cagatcatcg
      721 ggatcctcaa cgcctacatg aaggacccga aggcgcgctt cgccgaggac agcatcgatc
      781 tcgcccacgt cctcgcggcc gaagccagcg tcgcgatcgc gaatgcccag ctctacaagg
      841 aggccctcga cggacgcgag ttcgagcgcg cgctcgtgga atcgctcggc acgccggtgt
      901 tcgtcgcgcg tactcccgcg atggtcgcgg gcctgaaccg ggcggcggcc gacctgaccg
      961 gcatcgaccc ggtgcccgga gcggacgcgc agacgctgct gtcatcgctc ggcgcgcggc
     1021 tcgtggagac gggcgaggcc ctgacgcagt cgccgctcga catcggcctc gcgggccgcg
     1081 aatggcgtgg ggaggtcgcg ttctcccatc tcgtgacggg cgaggagcgc gtggcggacg
     1141 tcatcgtgca tccggtgcgc gaccccgagg ggaaggtgat cgccgcggtc gcgacgctgc
     1201 acgatctcac ggagctgcgc cgcgtcgagg aggagaagga gcagttcctc tcgatcgtgt
     1261 cccacgagct ccgcacaccg ctcacgccgc tgaaggccct cgcgcaactg ctcatcgcgc
     1321 gcatgcggcg ctcacgggag cgcggtaccc cgctcgatct ggactccttc gagcggaatc
     1381 tggcgtcgat cgaacggcag gtcgaccgca tgaacggact ggtgagcgac ctgctctcgg
     1441 tctcacgagc ggggcgcggc cagctggaga tggatcgccg gcccttcgat ctcgcgacga
     1501 gcgtgcgcga catcgtggag cgctacgtcg aagtgacgcg ggaagagggg cgccatcgtt
     1561 tcgcggtcga cgcgccgccg acgctggtcg tgacggccga ccaggcccgc gtcgagcaga
     1621 tgctgatgaa cctcgtcggg aacgccgtga agtactcgcc gcggggtggc gacgtgcgtg
     1681 tgtccctcga gcagcgcgac ggggtggccc agatcaccat caccgacgag ggcatcggca
     1741 tcagcagcga ggacctccct cgactcggga ccgccgcgtt cacccgcggc accggcaagg
     1801 ccgcgacgtt cgcgggtatg ggcatcggcc tgcacgtcgc caagctgttg gcggacgcgc
     1861 acggcggatc gctcgagctc gagagcgagg gtgaggatcg cgggacgacg gtgcgggtga
     1921 agctacccgc gtagcggtgc cggaggaggg acttgaaccc tcacgggctt gcgcccaaga
     1981 gattttgagt ctctcgcgta ctgccaattt cgccactccg gcgagaactc aattctaccg
     2041 gcgcagcatc tcgaacgccg cacgcgcgag agtcttgttc gcgatcacga cgcggccctc
     2101 gtcccacgcc gcatccttgc ccgtcttcag cgacagcacc gccgcgccgg cctcgcgtgc
     2161 gatgagcgcc ggcgccgcga tgtcccaggg cgacagcgcc tcgtgcgcga agagatcgaa
     2221 gcggcccatc gcgacgtacg cgatctcgag cgcggccgat ccgaagaccc gcaggcgggc
     2281 gcaggtggcg ttgagcgcga cgaagcgcga ctccggcctc ttcgtctcgc catagcgcag
     2341 gctcgcgacc gcgaacgcgt cggcaagacg cgcgacccga cttatcgcga tgcgctcgcc
     2401 attcagcgcg gcaccggcgc cctccgaggc gacgaatgtc tcgcgcgtgc gcggcgcatg
     2461 gaccgccgcc gcacgtgtgc ggccgtcctc gacgtacgcg atcaccacgc agtagaaggg
     2521 aaggccgcgg ctgaagttca gcgtgccgtc gatcggatcg acgacccaga gccgctccgc
     2581 gctccgaacg gaccgcttcg cgctctcctc ggcgagcacg gggatggcgg gatcgtgctc
     2641 cgcgaggcgc tggatgatcg cgccctcggc cgcgtggtcc gcggccgtgg cgaagttcgc
     2701 ccggccgcgc ttgcgcgtca cgatgccgat gtcactggtg acgatcgcgt cgccgacctg
     2761 ggtcgcggtg cgggccgcga gacgcgcgag gcgcgcgtag cggcgcgcgt ccgctccgct
     2821 caggacggac agagcgcgcg gaccttcgcg agcaggtcgt tcaggtcgaa cggcttcgcg
     2881 atgacgtcgt tgatcttgcg gcgcttgaac tcgtggccca tgtccttcga cgtcgcggtg
     2941 acgaacagca ccggcatgtt gcgcacgtcc ggatccgcgc ggacgcggtc ccacacctcg
     3001 aggccattca ggccaggcat catcacgtcg aggatgagaa ggtcggcatg gacctggcgg
     3061 accgtctcca ggacgagggc gccgtcatgg acgatgaccg cttgataggc tggctcgtca
     3121 ttgatggcat ctcgtaacag ccgggcgatc ggctcgtcgt cctcgccgat cacgacc
//
LOCUS       DASTZR010000281         9026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_955952, whole genome shotgun sequence.
ACCESSION   DASTZR010000281 DASTZR010000000
VERSION     DASTZR010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9026
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955952"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VFV20_06265"
     CDS             <1..302
                     /locus_tag="VFV20_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5287989.1"
                     /translation="SGRSRVARGVEVVPTPGHAPGHQSLVVDLAETGRVVLTGDAAFT
                     RANLEHGDIPAFDQAAARESLALIRSLVRDDLSRVFTSHDADAWETWRHAPESYR"
     gene            272..883
                     /locus_tag="VFV20_06270"
     CDS             272..883
                     /locus_tag="VFV20_06270"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEU5287990.1"
                     /translation="MASRAGVVPLTGATLILLAGGRSTRMGRDKASLRVGEGTLVEWI
                     VRRLGPSFSETLVCGGSAPGARGVPDRRPDAGPLAGIEAGLLAMRTEAAFVLACDMPR
                     VSERLATLLLERLADHDIAMPRVGEMDQPACAAYARRVTTRLTEYLGGGGRRVSAFIA
                     TLDVLRVDERALADAGVSLEELVDLDTPAAYDAFVTSLRGPRD"
     gene            893..1741
                     /gene="fdhD"
                     /locus_tag="VFV20_06275"
     CDS             893..1741
                     /gene="fdhD"
                     /locus_tag="VFV20_06275"
                     /inference="COORDINATES: protein motif:HMM:NF001943.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEU5287991.1"
                     /translation="MARASSRRIGIARYDGGAWDATGDDIAAEEPLQLLLDGEPLSVI
                     MRTPGNDIELALGLLHAERVITSLDDVASLRISAEADETEGRVRVSADLVESNAVDVH
                     LAKTAARRPRRSFLSSSACGVCGATTMEDLAHDHAPLAAGPVVDASALPPLAERLRER
                     QRLFDLTGGLHAAGLFRADGELLTLREDVGRHNAVDKVVGRAVLDRSVPLAGTMLAVS
                     GRGGYELVQKAVAAGIPILVAVGAPSSLAVATALRFGLTLVGFLRGERFNVYSRPERI
                     RGAPPG"
     gene            1754..2365
                     /locus_tag="VFV20_06280"
     CDS             1754..2365
                     /locus_tag="VFV20_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287992.1"
                     /translation="MSTVFRCVLCRRADRPMTREHVFARWLIARVHGAKLVSRAAIPD
                     PRISRVIAPVCADCNAGWMSGLEVGFRRTVFARPRSGVVQPPERTLLSRWFTKSAVLV
                     ADAHGLSLGSLEHRPAVVTGMPADIEVFVGRLRRPRQAIDFVLVPGDADDARAVAISV
                     RDLVGHVAVRGTLTSRHGTKLWPLRTHALRWETLPVIPGLADA"
     gene            complement(2403..2885)
                     /locus_tag="VFV20_06285"
     CDS             complement(2403..2885)
                     /locus_tag="VFV20_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287993.1"
                     /translation="MSSRLAEPSVGRVPDIGVRLALAGDPGELFADARALEAAGAESL
                     WIDASDGDPYVALAAMSVVTSRVRLVARGAPEGSGRETCAALCRGRLRVAEELERGGE
                     RWTLAAFPADRKAWRALREAAAAAGTSGIVIPNDPRLIDLLRNPDQEDDRSDLNIAVG
                     "
     gene            complement(2866..3156)
                     /locus_tag="VFV20_06290"
     CDS             complement(2866..3156)
                     /locus_tag="VFV20_06290"
                     /inference="COORDINATES: protein motif:HMM:NF040429.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6457 domain-containing protein"
                     /protein_id="HEU5287994.1"
                     /translation="MNDWFSSLAARWVKAGSGRGAKISPPDLDPAVAEELLQLARVVA
                     HQKERSYAPLASFTAGVAVERLRSANALGEPAEVAAYIREVREALEREQPAG"
     gene            3224..6367
                     /gene="fdnG"
                     /locus_tag="VFV20_06295"
     CDS             3224..6367
                     /gene="fdnG"
                     /locus_tag="VFV20_06295"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:3812..3814,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HEU5287995.1"
                     /translation="MSELTRRQLLKAGAGSAGLALVPLANPLGFDLREAKRIQVEFRL
                     SGAKEVHSICPYCAVGCSLIAYTKEVNGKTQLLQIEGDPDSPVNEGRLCPKGATAMQL
                     ATSSRRVEKPLYRAPGSGKWEEKSWDFMLDKLAKNIKSSRDNTFVAKDANGNTVNRTE
                     GVAFAGGAAFSSEEGYFATKLMRSLGVVHIEQQARVUHGPTVVSLAATFGRGAMTNHW
                     RDIKHADVILINGANPAEAHPVGFQWFVKAKLDTTKGPGNGGGAKIIHADPRYTRTSA
                     LADRYLRIRTGTDVAYFGGLINYVLQNKLYHEEYVKNYTNASFIVKKEFSFNDGLFSG
                     YNKNTRSYDTSSWAYEGTTPPDATQSKGGQEGGGQGGTGADQFAKRDMTLEDPQTVFQ
                     LLKKHYSRYTPEMVESITGIPKDQFLEVAKIVGEMGRPDKVMTIVYAVGLTHHTTGGQ
                     LIRSGALLQLLLGNMGRPGGGMNAERGHANIQGNTDHAISWEILPGYLRIPSPGQKNL
                     DEYVKASAPKKSDPNSWNFFGTNYLNFMVSLLKGWYGNAATASNEYAFEFIPKPAVNS
                     SWMSIYDQALKGKMEGVILSGMTATSIGPDSNQVQDALAKLKWLVVMDPLPTTSSEFW
                     RAPGKDPAAIQTEVFMLPTTHWIEKDGSFVNSGRWMQWKDAVLHAEGEARHDHWILAE
                     VYDRVKTLYKQEGGKFPDPINALVLDYKDPKKPTLEEIAKEINGKDLTTGKQMASFAN
                     LKSDGTTTAGDWIYTGSWTEDGNLANRRNGIQDPAKNDPTGMGFYPQWAWSWPLNRRV
                     MYNRASADVNGKPWDPKRPAIQWDGTRWQGDVPDYPATMDPKDPKAWLPFIMNGEGVG
                     RLFSNSMNDGPFPEHYEPVESPIPNPLHPNQSEDPAAFLYDQAAGRPNRFGKPDQFPY
                     VATSYRLTEHEHYVTQHVPLLVGLQPHAFVEVPEELANEKGIKNGDRVRVSSMRGKLE
                     VAALVTRRLGPLTVGGKKTYTIGIPIHWGFVGVSAELYPDHSKNWLANALTPFVGDAN
                     ARTPEFKAFLVNIEKIT"
     gene            6364..7152
                     /locus_tag="VFV20_06300"
     CDS             6364..7152
                     /locus_tag="VFV20_06300"
                     /inference="COORDINATES: protein motif:HMM:NF016131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HEU5287996.1"
                     /translation="MSAVAAASTVFGQRAQRAADLAERYSYAAEPLALYRAVLGAQER
                     AYLRARDEHVDDLVAYVVRTSLPSVMDVTMSTGTETLREAVLLRFHEGDLDRIVRAWL
                     AGEDLDETDRYLARAAAAPVLEAHPELAASLRRDGADERHCPTCGGLPQLAVFADTGE
                     ALVTGQRRLVCSRCATEWTYARLVCVSCRETNGAKMPILADEARLPHIRADACDTCHA
                     YLITVDVRRDPRAVPLVDEIAALPLDLLASERGYTKIARNLMGF"
     gene            7153..8010
                     /locus_tag="VFV20_06305"
     CDS             7153..8010
                     /locus_tag="VFV20_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU5287997.1"
                     /translation="MATIEARPVGFFTDTSICIGCKACEVACKEWNQLEGNTPSFLAD
                     SFDNTGQLDAQNWRHVQFTETIPTTNGAENGGSHGAVVNTPTTAGGQAWLMMSDVCKH
                     CQQASCLEVCPTNAIVRTEFDTVLIQQSVCNGCRNCISACPYAVIGFNEKTGTAHKCT
                     LCYDRLQNGMQPACAKACPTESIKFGYLDDLHAAADKRLAALQTAGFKQAQLYGRDAS
                     VYGGLNAFFLLMDKPETYKLPNKENATLPSRNNVPGYLGAAVTAVAGLFAGVLAFRSN
                     RMSTMEKRR"
     gene            8027..8917
                     /gene="nrfD"
                     /locus_tag="VFV20_06310"
     CDS             8027..8917
                     /gene="nrfD"
                     /locus_tag="VFV20_06310"
                     /inference="COORDINATES: protein motif:HMM:NF025940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEU5287998.1"
                     /translation="MTGAEHFAASPNWEWWILLYFFFGGIAGGSYAIGTLIRLVGTAA
                     DQRIARVAFIVSFLALIPCPIFLIVDLGAPLRFLNMIFDASEGGLAFKPWSPMSVGSW
                     ALLGFGLFSFVSFLGALGESMMPALEPVTRLLRGTVGAVWSVIGTGLGFFVAGYTGVL
                     LAVSNQPVWSDSGWVLGGLFLASGLAGAAALLLLLAGSRRDVDAGATTRLEIADRNFV
                     TLEAILLVLFVVSVAVAGTLGKLLGLWGLLWIVIVLGLAAPFVARSAAMRTWPPVAAP
                     IVALLGVLALRALVIFGAQS"
BASE COUNT         1425 a         3160 c         3091 g         1350 t
ORIGIN      
        1 tgagcgggcg ctcgcgtgtg gcgcgcggcg tggaggtcgt gccgacgccc ggtcacgcac
       61 ccggccacca gtcgctcgtg gtggacctcg ccgagaccgg acgtgtcgtg ctgaccggcg
      121 atgccgcgtt cacgcgcgcg aatctcgaac atggcgacat ccccgccttc gaccaggccg
      181 cggcacggga gtcgctggcg ctgatccgct cgctcgtacg cgacgacctg tctcgcgtct
      241 tcacgtcgca cgatgccgac gcatgggaga cgtggcgtca cgcgccggag tcgtaccgct
      301 gacgggcgcg accctcatcc tgctcgccgg cggccggtcg acgcggatgg gccgcgacaa
      361 ggcgtcgctg cgcgtcggcg agggcaccct cgtcgaatgg atcgtgcgcc gtctgggccc
      421 gtcgttctcc gagacgctgg tgtgcggcgg gtcggcgccg ggcgcgcgcg gtgtccccga
      481 ccggcgccct gatgcgggcc cgctggccgg catcgaggcc gggctcctcg cgatgcgtac
      541 cgaggcggcg ttcgtgctcg cgtgcgacat gccccgcgtt tccgagcggc tcgcgaccct
      601 cctcctcgaa cggctcgcgg accacgacat cgcgatgccc cgcgtcggcg agatggatca
      661 gcccgcatgc gccgcctatg cacgccgggt gacgacgcgg ctcaccgagt acctcggcgg
      721 cgggggccgg cgggtcagcg cgttcatcgc gacgctcgac gtcctccggg tcgatgagcg
      781 cgcactcgcg gatgccggcg tctccctcga ggagctcgtg gatctcgaca cgcccgcggc
      841 gtacgacgcg ttcgtgacat cgctgcgcgg gccgcgcgac taacctcttc ggatggcgcg
      901 cgcatcgtca cgacgcatcg ggatcgctcg gtacgacggc ggcgcgtggg acgcgacggg
      961 agacgacatc gcggccgagg agccgctgca gctgctgctc gacggcgagc cgctcagcgt
     1021 gatcatgcgc acgccgggca acgacatcga gctcgcgctc ggcctgctcc acgcggagcg
     1081 cgtcatcacc tcgctcgacg acgtcgcgtc ccttcggatc tcggccgagg cggacgagac
     1141 cgaaggccgc gtgcgcgtga gcgcggatct cgtggagtcg aacgcggtcg acgtgcatct
     1201 cgcgaagacc gccgcgcgcc ggccgcgccg gtcgttcctg tcgagctccg cgtgcggcgt
     1261 ttgcggcgcg acgaccatgg aggacctcgc gcacgaccat gcgccgctcg ccgccggtcc
     1321 ggtcgtcgat gcgtccgcgc tgccgccgct cgccgagcgt ctgcgcgagc ggcagcgcct
     1381 cttcgacctc accggcggtc tgcacgcggc cggtctcttt cgcgcggacg gcgagctcct
     1441 cacgctgcgt gaggacgtcg gccggcacaa cgccgtggac aaggtcgtcg gacgcgccgt
     1501 gctcgaccgg agcgtgccgc tcgccgggac gatgctcgcc gtgagcgggc gcggcggcta
     1561 cgagctcgtg cagaaggcgg tcgccgcggg catcccgata ctcgtcgcgg tgggcgcgcc
     1621 gtcatcgctc gccgtcgcga ccgcgctgcg gttcggactc accctggtcg gcttcctgcg
     1681 gggagagcga ttcaacgtct acagccggcc cgagcggata cgcggcgcgc cgcctggcta
     1741 gccttcgccg ccgatgagca cggtcttccg atgcgtcctg tgccggcggg ccgaccggcc
     1801 gatgacgcgg gagcacgtct tcgcccggtg gctgatcgcg cgcgtccacg gcgcgaagct
     1861 cgtctcgcgc gcggccatcc ctgatccacg catctcgcgc gtcatcgcac cggtgtgcgc
     1921 cgactgcaac gcgggttgga tgagcggtct cgaggtgggc ttcaggcgga ccgtcttcgc
     1981 tcgccctcgg tccggcgtgg tccagcctcc ggagcgcacg ctcctcagtc gctggttcac
     2041 caagagcgcc gtgctcgtcg ccgacgcgca cgggctctcg ctcggcagcc tcgagcatcg
     2101 cccggccgtc gtcactggca tgcccgccga catcgaggtc ttcgtcggac ggttgcggcg
     2161 gccgcgacag gcgatcgact tcgtgctcgt acccggcgat gccgacgacg cccgcgccgt
     2221 cgccatctcg gtgcgcgacc tcgtgggaca cgtggccgtc cgcgggacgc tcacgagccg
     2281 tcacgggacg aagctctggc cgctgcgcac gcacgcactg cgctgggaga cgctgccggt
     2341 gatccccggc ctcgctgacg cctagtgtcg cgtctcggaa ttcatgcgca gctgccgcga
     2401 cactagccga cggcgatgtt gaggtcggag cggtcgtcct cttgatcggg attccgcagc
     2461 aggtcgatga gacgtggatc gttcgggatg acgatgccgg aggtgcccgc cgcggccgcc
     2521 gcctcgcgaa gcgcgcgcca cgccttccga tccgcgggga aagccgcgag cgtccagcgc
     2581 tcgccgccgc gctcgagctc ctccgcgacc ctgagacggc cgcggcacag cgcggcgcag
     2641 gtctcgcgtc ccgagccctc cggggcgccg cgcgcgacca gacgcacgcg cgaggtgacc
     2701 accgacatcg cggcgagcgc gacgtacgga tcgccgtcgc ttgcgtcgat ccagagtgac
     2761 tcggcaccgg cggcctcgag cgcccgagcg tcggcgaaca gctcgccggg gtcgcctgcc
     2821 agtgcgagcc gcacaccgat gtcgggcacg cggccgaccg acggctcagc cagccggctg
     2881 ctcacgctcg agcgcctcgc gcacttcgcg gatgtaggcg gcgacctccg cgggctcccc
     2941 gagagcgttc gccgaccgca gccgctcgac cgcgacaccg gcggtgaagc ttgcgagagg
     3001 cgcgtagctg cgctcttttt gatgggccac gacgcgcgcg agctgcagca gctcctcggc
     3061 gaccgcgggg tccaggtccg gcggcgagat cttcgcgccg cgaccggaac cggccttcac
     3121 ccaccgcgct gcgagagatg aaaaccagtc attcaccggt cgccaaagta gcgcgttggc
     3181 tcacttgacg cgctccgcgt ggggagtaca acgttcctca ggcatgtcgg aactgacgcg
     3241 acgtcagctt ctgaaggcag gtgcgggaag cgccggcttg gccctcgtcc cgctcgccaa
     3301 cccgctcggc ttcgatcttc gtgaggcaaa gcggatccag gtcgagttcc gcctttcggg
     3361 cgcgaaggaa gtccattcca tctgtcccta ttgcgcggtc ggctgctcgc tcatcgccta
     3421 cacgaaagag gtcaacggca agacgcagct gctgcagatc gaaggtgatc ccgacagccc
     3481 ggtgaacgag ggtcgcctct gcccgaaggg cgccaccgcg atgcagctcg cgacgagctc
     3541 gcgccgcgtc gagaagccgc tctatcgcgc gcccggcagc ggcaagtggg aagagaagtc
     3601 ctgggacttc atgctcgaca agctcgcgaa gaacatcaag tcgtcacggg acaacacgtt
     3661 cgttgccaag gatgccaatg ggaacaccgt gaaccggacc gaaggcgtgg ccttcgccgg
     3721 cggcgcggcg ttctcgtccg aggaggggta cttcgccacc aagctcatgc ggagcttggg
     3781 cgtcgtacac atcgagcagc aagctcgggt ctgacacggt cccacggtgg tcagtttggc
     3841 cgccacattc ggacgaggag cgatgacgaa ccattggcgt gacatcaagc acgccgacgt
     3901 catcctcatc aacggcgcga acccggccga ggcgcatccc gtcggcttcc agtggttcgt
     3961 gaaagcgaag ctcgacacca cgaagggccc ggggaacggc ggcggcgcga agatcatcca
     4021 cgccgacccg cgctacacgc gcacctcggc gctcgccgac cgctacctgc gcatccgcac
     4081 cggcaccgac gtggcgtact tcggcgggct catcaactac gtgctgcaga acaagctcta
     4141 ccacgaggag tacgtcaaga actacacgaa cgcgtccttc atcgtgaaga aggagttctc
     4201 attcaacgac ggtctcttca gcggctacaa caagaacacc cgcagctacg acacctcgtc
     4261 gtgggcgtac gagggcacca cgccgcccga cgcgacgcag tccaagggcg gtcaggaggg
     4321 cggcggccag ggcgggaccg gcgccgatca gttcgccaag cgcgacatga ccctcgagga
     4381 cccgcagacg gtcttccagc tcctgaagaa gcactacagc cgctacacgc cggagatggt
     4441 cgagagcatc accggcatcc cgaaggacca gttcctcgag gtcgcgaaga tcgtcggcga
     4501 gatggggcgt cccgacaagg tgatgaccat cgtgtacgcc gtcggcctca cgcatcacac
     4561 gacgggcggc cagctcatcc ggtcgggtgc attgctgcag ctgctcctcg gcaacatggg
     4621 ccgcccgggc ggcggcatga acgccgagcg cggtcacgcg aacatccagg gcaacaccga
     4681 tcacgcgatc tcgtgggaga tcctgcccgg ctatctccgc atcccttcgc ctgggcagaa
     4741 gaacctcgac gagtacgtga aggcgagcgc gcccaagaag agcgacccga actcctggaa
     4801 cttcttcggg acgaactacc tgaacttcat ggtgagcctg ctgaagggct ggtacggcaa
     4861 tgccgcgacc gcgtcgaacg agtacgcgtt cgagttcatc ccaaagccgg cggtgaactc
     4921 gtcgtggatg tccatctacg accaggcgct caaggggaag atggagggcg tcatcctctc
     4981 ggggatgacc gcgacgagca tcgggccgga ctcaaaccag gtgcaggacg cgctcgcgaa
     5041 gctgaagtgg ctggtcgtga tggacccgct gccgacgacg agctcggagt tctggcgcgc
     5101 gccgggtaag gacccggcgg cgatccagac cgaggtcttc atgctgccga cgacgcactg
     5161 gatcgagaag gacggctcct tcgtcaacag cggacgctgg atgcagtgga aggacgcggt
     5221 cctccacgcg gagggcgagg cgcggcacga ccactggatc ctcgccgagg tctacgaccg
     5281 cgtgaagacg ctctacaagc aggagggcgg caagttcccc gacccgatca acgcgctggt
     5341 cctcgactac aaggacccga agaagccgac gctcgaggag atcgcgaagg agatcaacgg
     5401 caaggacctc acgacgggga agcagatggc gtcgttcgcg aacctgaagt cggacggcac
     5461 gacgaccgcc ggcgactgga tctacacggg cagctggacc gaggacggca acctcgccaa
     5521 ccggcgcaac ggtatccagg atccggcgaa gaacgacccg acgggcatgg gcttctatcc
     5581 gcagtgggcc tggagctggc cgctcaaccg gcgtgtcatg tacaaccgcg cgtccgccga
     5641 cgtgaacggg aagccgtggg accccaagcg tcccgcgatc cagtgggacg gcacgcgctg
     5701 gcagggcgac gtgcccgact acccggcgac gatggatccg aaggatccca aggcgtggct
     5761 gccgttcatc atgaacggcg agggcgtcgg ccgcctcttc tcgaactcga tgaacgacgg
     5821 cccgttcccc gagcactacg agcccgtcga gtcgccgatc ccgaacccgc tgcacccgaa
     5881 tcaatcggag gatccggccg cgttcctcta cgaccaggcc gcgggccgtc cgaaccgctt
     5941 cggcaagccc gaccagttcc cgtacgtcgc cacgagctac cggctcaccg agcacgagca
     6001 ctacgtgacg cagcacgtgc cactgctcgt cggactccag ccgcacgcgt tcgtcgaggt
     6061 gcccgaggag ctggccaacg agaagggcat caagaacggt gaccgcgtgc gcgtgtcgag
     6121 catgcgcggc aagctcgagg tcgccgcgct cgtcacgagg cgcctcggcc cgctcaccgt
     6181 cggcgggaag aagacgtaca cgatcggtat cccgatccac tggggcttcg tcggcgtttc
     6241 ggccgagctg tatccggacc actcgaagaa ctggctcgcg aacgcgctga ccccgttcgt
     6301 cggtgacgcg aacgcgagga cgccggagtt caaggcgttc ctcgtgaaca tcgagaagat
     6361 cacatgagcg cggtcgcggc ggcgagcacc gtcttcgggc agcgcgccca acgcgccgcg
     6421 gatctggccg agcgctactc gtacgcggcc gagccgctcg cgctgtaccg ggccgtgctc
     6481 ggcgcgcagg aacgggccta cctgcgggca cgggacgagc acgtcgatga cctcgtcgcg
     6541 tacgtcgtcc ggacgtcgct gccatcggtg atggacgtca cgatgtccac gggtacggag
     6601 acgctccgtg aggccgtgct gctccgcttc cacgagggcg acctcgaccg catcgtgcgc
     6661 gcgtggctcg cgggggagga cctcgacgag accgaccgct acctggcgcg cgccgcggcc
     6721 gcaccggtgc tcgaggcgca tccggagctc gcggcgtcgc tccgtcgcga cggcgccgac
     6781 gagcggcact gcccgacctg cggcgggctc ccgcagctcg cggtcttcgc ggacacgggc
     6841 gaggcgctcg tcaccgggca gcgtcgcctc gtctgctcgc gctgcgcgac ggaatggacg
     6901 tacgcgcgcc tggtgtgcgt cagctgccgc gagacgaatg gcgcgaagat gccgatcctc
     6961 gcggacgagg cacgcttgcc gcacatccgg gcggacgcgt gcgatacctg ccacgcctac
     7021 ctgatcaccg tcgacgtccg cagggatcca cgagccgtac cgctcgtgga cgagatcgcg
     7081 gcgctcccgc tcgatctgct cgcgtcggaa cgcgggtaca cgaagatcgc gcggaacctg
     7141 atggggttct aggtggcaac gatcgaagcg cgccccgtcg gcttcttcac tgacacgtcg
     7201 atctgcatcg gctgcaaggc ctgcgaggtc gcgtgcaagg agtggaacca gctcgagggc
     7261 aacacgccgt cgttcctggc cgacagcttc gacaacaccg gccagctcga cgcccagaac
     7321 tggcgtcacg tgcagttcac ggagacgatc ccgacgacga acggcgcgga gaacggcggg
     7381 agccatggcg ccgtcgtcaa cacaccgacg accgcgggcg gccaagcgtg gctgatgatg
     7441 tccgacgtct gcaagcactg ccagcaggcg tcgtgtctcg aggtctgtcc gacgaacgcc
     7501 atcgtccgca cggaattcga cacggttctc atccagcagt cggtctgcaa cggctgccgc
     7561 aactgcatct cggcgtgtcc ctacgccgtc atcggcttca acgagaagac cgggaccgcg
     7621 cacaagtgca cgctctgcta cgaccggctg cagaacggga tgcagccggc gtgcgcgaag
     7681 gcctgcccga ccgagtccat caagttcggc tacctcgacg acctgcatgc ggcggcggac
     7741 aagcggctcg ccgcgctgca gaccgccggc ttcaagcagg cgcagctcta cggacgcgac
     7801 gcgtcggtgt acggcggcct caacgcgttc ttcctgctga tggacaagcc cgagacgtac
     7861 aagctgccga acaaggagaa cgcgacgctt ccgagccgca acaacgtccc cggatacctc
     7921 ggcgccgccg tgacggcggt cgcggggctc ttcgcgggcg tactcgcgtt ccggagcaat
     7981 cggatgagca cgatggagaa gcggcgatga aggccggtga tcgaccatga ccggtgccga
     8041 acactttgcg gcatcaccga actgggagtg gtggatcctc ctgtacttct tcttcggtgg
     8101 gatcgccggc ggaagctacg cgatcggaac gctcatcagg ctcgtcggca cggccgccga
     8161 ccagcgcatc gcgagggtcg cgttcatcgt cagcttcctc gcgctcatcc cgtgcccgat
     8221 cttcctcatc gtcgacctgg gcgccccgct ccgcttcctc aacatgatct tcgacgcgag
     8281 cgaaggcggg ctcgcgttca agccgtggtc gccgatgtcg gtcggctcgt gggcgctgct
     8341 cgggttcggg ctcttctcgt tcgtctcgtt cctcggagcg ctcggcgaga gcatgatgcc
     8401 cgcgctggag ccggtgacgc ggctgctgcg cggcacggtc ggcgcggtct ggagcgtcat
     8461 cggcaccggg ctcggcttct tcgtcgcggg ctacacgggc gtgctcctcg cggtctcgaa
     8521 ccagcccgtc tggagcgact ccggctgggt gctcggcggc ctcttcctcg cgtccggcct
     8581 cgccggcgcc gcggcgctcc tgctgctgct cgccggctcg cggcgcgacg tcgacgccgg
     8641 cgccaccacg cggctcgaga tcgccgaccg caacttcgtg acgctcgagg cgatcctgct
     8701 cgtgctcttc gtcgtgtcgg tggcggtcgc gggcacgctc ggaaagctcc tcggtctgtg
     8761 gggcctgctg tggatcgtca tcgtgctcgg cctcgccgcg ccgttcgtcg cgcgctccgc
     8821 cgcgatgcgc acctggccgc cggtcgccgc gccgatcgtc gccctgctcg gcgtgctcgc
     8881 gctccgcgcg ctcgtgatct tcggcgcgca gagctagctg cggcctccgc ggcggcgagt
     8941 cgcgtccgcg tggcggcggg tggcacgcgc agggcgtcgt tctcaccgcg aagtccgtgg
     9001 ggccccaggg gcaggggccc tgtgta
//
LOCUS       DASTZR010000282         2154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_302986, whole genome shotgun sequence.
ACCESSION   DASTZR010000282 DASTZR010000000
VERSION     DASTZR010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302986"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VFV20_06315"
     CDS             complement(<1..579)
                     /locus_tag="VFV20_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5287999.1"
                     /translation="MLALGTRAQADDPAPRAIPAPRAYERAFSSYEALVTYATTVADA
                     QTALDAQLAETRASLDEIVARIGAIAAARGRGAHLDLEAAAVWRRLRTEETVLGARAT
                     TLREAQQRLLAAGALTGAVTPWRMPTEGEITQEFGPTDLWVEPARDFGGVAYTHFHEG
                     VDIAGAWSAPVVAPARGRVVFVGAMSDGAELVV"
     gene            complement(725..1885)
                     /gene="fni"
                     /locus_tag="VFV20_06320"
     CDS             complement(725..1885)
                     /gene="fni"
                     /locus_tag="VFV20_06320"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEU5288000.1"
                     /translation="MRRAGGRGGVQVTGGAEDIARRKGEHLQLAAEGDVETRASPGWE
                     DVHLIHDALPSIDAAAVDLSATFLGHKLRLPLVISGMTGGHGRAIAVNELLARVAERA
                     GIAIGVGSQRAALRDPSLIPTYAVVREAAPHAFVIANVGISQLVKQGSEPALAAKDVR
                     TIVQMVGADALALHLNYLEESVQPEGQTRATGSEAAVRALVKASPVPVIAKETGAGIS
                     RAVALRLRRLGIAAIDVGGVGGTSFAAIEALRAASAGDALRMSLGERFRDWGLPTAVA
                     VVGASGARLPVIATGGVRSGLDAAKALALGATVVGVGRPLLQAALQGEAACLAWLAAF
                     ELELRTAVFLTGIKRASNLRNTPVVLTGRSRAWIDQLAYRRANIGSSRARSS"
     gene            complement(1849..>2154)
                     /locus_tag="VFV20_06325"
     CDS             complement(1849..>2154)
                     /locus_tag="VFV20_06325"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU5288001.1"
                     /translation="QPRERAVARLRSHGLRAAPVESIGELFHDPQLAERRAWRTATHP
                     AIGEVRLMAPPFLLSDAEQGPTRPGPLLGQHTEEVFRGIVGLDAAEYAALEAAGVFR"
BASE COUNT          311 a          781 c          771 g          291 t
ORIGIN      
        1 gacgacgagc tcggcgccgt cggacatcgc tccgacgaag acgacgcgac cgcgtgccgg
       61 cgcgacgacc ggtgcggacc acgcgccggc gatgtcgaca ccctcgtgga agtgcgtata
      121 tgcgacgccg ccgaaatcgc gagccggttc gacccacagg tcggtcggcc cgaactcctg
      181 cgtgatctcg ccttcggtgg gcatgcgcca gggcgtcacc gcgcccgtca gtgcgcccgc
      241 ggcgaggagc cgctgctgcg cctctcgaag cgtcgtcgcg cgtgcgccca gcaccgtctc
      301 ttcggtgcgc agccggcgcc acaccgcggc cgcctcgagg tcgaggtgcg ctccgcgtcc
      361 gcgcgcagcg gcgatggcgc cgatcctcgc gacgatctcg tcgagcgacg cgcgggtctc
      421 cgcgagctgg gcgtcgagcg cggtctgtgc gtctgcgacc gtcgtcgcat aggtgaccag
      481 cgcttcgtat gagctgaagg cccgttcgta agcgcgcggg gcgggaatgg cgcgcggcgc
      541 cgggtcgtcc gcctgcgcgc gcgtacccag cgccagcaag cagctgaccg ctacgcagag
      601 ggagaggagg gcgcgcgcaa gcgcgttaca agatcgcaat gcggtgcaga catcgtccag
      661 gagaagggtc ggtcagccgt cacttgcaga ggatccgatc gcgtgagaaa tggctcgctc
      721 gagctcagga cgagcgagcc cgcgacgagc caatgtttgc gcggcgatac gcgagttgat
      781 cgatccacgc gcgcgatcga cccgtgagga ccaccggagt gttgcgcaga ttcgatgcac
      841 gcttgatacc ggtcaagaag acggctgttc gcagctcgag ctcgaacgcc gccagccatg
      901 ccaggcacgc cgcctccccc tgcagcgcgg cctgcagcag cgggcgaccg acgccgacga
      961 cggtcgcccc gagtgcgagc gccttcgcgg cgtcgaggcc gctccggacg ccgcccgtcg
     1021 cgatcaccgg aagacgtgcg cccgacgcac ccaccacggc gacggcggtc gggagacccc
     1081 agtcgcggaa ccgctcgccg aggctcatgc gcagggcatc gccagccgac gcggcgcgca
     1141 gcgcctcgat cgccgcgaag gacgttccgc ccacgccgcc gacgtcgatc gcggcgatgc
     1201 ccagacgtcg gaggcgcagc gcgacggccc gtgagatgcc ggcgcccgtc tctttggcga
     1261 tgaccggcac gggcgacgcc ttcacgagcg cgcggacggc ggcctccgat ccggtcgcgc
     1321 gtgtctgtcc ctccggctgc accgattcct cgaggtagtt gagatggagc gcgagcgcgt
     1381 cggcgcccac catctgcacg atcgtcctga cgtccttggc cgcgagggcc ggttcactcc
     1441 cctgcttcac cagctgcgag atgccgacgt tcgcgatgac gaaggcgtgc ggcgcggcct
     1501 cgcggacgac ggcgtacgtc gggatcaggc tcggatcgcg aagggccgcg cgctggctgc
     1561 ccaccccgat cgcgatgccg gcccgctcgg ccacgcgcgc gaggagctcg ttcaccgcga
     1621 tcgcgcggcc gtggccgccg gtcatcccgg agatcaccag tgggagacgc agtttgtggc
     1681 cgaggaacgt cgcgctcaga tccaccgccg ccgcgtcgat gctgggcagc gcgtcgtgga
     1741 tgaggtgcac gtcctcccag ccgggggacg cgcgcgtctc cacgtcgccc tcggcggcga
     1801 gctgcaggtg ctcccctttt cggcgagcga tgtcttccgc gccgcctgtt acctgaacac
     1861 ccccgcggcc tccagcgcgg cgtattccgc cgcgtcgaga ccgacgatgc cacggaacac
     1921 ctcttcggtg tgctggccga gcagcggacc cggccgggtg ggtccctgct cggcgtcgga
     1981 tagcaggaag ggcggcgcca tcaggcgcac ctcaccgatc gccgggtgcg tcgccgtgcg
     2041 ccaggcgcgg cgctccgcga gctgcgggtc gtggaagagc tcgccgatcg actcgaccgg
     2101 cgccgctcga aggccgtgcg accgaagccg cgcgaccgcg cgctcgcgcg gctg
//
LOCUS       DASTZR010000283         4025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_903815, whole genome shotgun sequence.
ACCESSION   DASTZR010000283 DASTZR010000000
VERSION     DASTZR010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903815"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VFV20_06330"
     CDS             <1..819
                     /locus_tag="VFV20_06330"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5288002.1"
                     /translation="LLITIASRLGPGLGIDPIALAWLLVPAVLLPGMALVFLIHPDPR
                     DIAKDLGAYYGPDVVLTQAAAAHGPSGNGVRDWLAHYPLRVAFVASFAAQGVMSLMMA
                     MTSLALDHHGHGLQAISISVSIHVVGMYALSIPLGHLSDRFGRRNVMLAGTVLSALGS
                     ILVGVATEYAPITIGTFLVGVGWSCTNVSTSALIADVVAPEDRGRAIGTCDTFSGAGS
                     VALPLLGGPLVEIVGFPILAVVGTLLLTLPFAMLLRLREGTRREHAAVVAEAAD"
     gene            complement(891..1019)
                     /locus_tag="VFV20_06335"
     CDS             complement(891..1019)
                     /locus_tag="VFV20_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288003.1"
                     /translation="MAFSIGLFAGWLALVVWLVWPDPHCGCCAKRAADRTARDQRF"
     gene            complement(1079..1816)
                     /gene="hisA"
                     /locus_tag="VFV20_06340"
     CDS             complement(1079..1816)
                     /gene="hisA"
                     /locus_tag="VFV20_06340"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEU5288004.1"
                     /translation="MIVYPAIDIYEGRVVRMTRGDFDTLEQVAPSPLEAARRLVNDGA
                     EWVHVVDLDGARTGTPANLDAIRQIANRFTVKIQAGGGIRDFDTAERFAEAGASRIVV
                     GTAAVEEPELIGRLVDRHADGMAVAVDARGGMVTTAGWTETTTTKAVDLMHRLAVEGV
                     PIVIYTNVSVDGTLQGVDLASLEAVARAFGGDVIYSGGVGSLDDIRALARLRHRGIRG
                     VIIGRALYAGKFTLAQAIAAVGSVPAS"
     gene            complement(1839..2213)
                     /locus_tag="VFV20_06345"
     CDS             complement(1839..2213)
                     /locus_tag="VFV20_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288005.1"
                     /translation="MTRDVDMPERDEEVREDQVRDEQVREERREPQDESGGGLFASER
                     ADELRARWTDVQAQFVDDPKAAVKAADGLVEEVLRDLAALFTDERDRLGQQLGAKQRL
                     DTEQLRVAIRRYRAFFEKLLSV"
     gene            complement(2210..2788)
                     /locus_tag="VFV20_06350"
     CDS             complement(2210..2788)
                     /locus_tag="VFV20_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288006.1"
                     /translation="MDQQQLIVAAILVLAFVAIAVIWYAMRQRRREALRERFGPEYDH
                     VVRQEGDADRAERVLNDRQQRVEQLHVRPLRAQDAAAFRERWRAVQARFVDDPRGAVS
                     DADDLIGQAMEARGYPVGDFEQRAADVSVEHPQVVSDYREAHAIARRPAQKTGTEELR
                     RAMVHYRALFAELVGAGTRPGERVEEREEQLR"
     gene            complement(2850..2978)
                     /locus_tag="VFV20_06355"
     CDS             complement(2850..2978)
                     /locus_tag="VFV20_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288007.1"
                     /translation="MEVISLIVGIGIGTALATLIIAFTAIGQYQRGYDAGRKAFRL"
     gene            complement(3073..3903)
                     /locus_tag="VFV20_06360"
     CDS             complement(3073..3903)
                     /locus_tag="VFV20_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012381590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HEU5288008.1"
                     /translation="MPPVHAPDGTLVNAIVGFLNFVARLLTDRRPARCVVAFEFGEDW
                     RPQFRVTALPEYKSHRVAEEGAPPDEVGPQIVVIEEVLRAIGIATANAGECEAEDVIA
                     TLATRYDGPVEVVSGDRDLYSLVRDPRITVLYTLKGVSELAHVDQAWVHAKYGIPGDR
                     YLDYAILRGDPSDGLPGVRGIGEKTAAALLTKYGSLEGILAATDLSATIKAKLNAGRD
                     YIVAARRVVSPVLDCHVPEISGALPSGVPRADIDALAKRYGIEGSTRRVLDALRGLAT
                     "
BASE COUNT          584 a         1459 c         1380 g          602 t
ORIGIN      
        1 ctcctcatca cgatcgcgag ccggctcggt cccggtctcg gcatcgaccc gatcgcgctc
       61 gcgtggctgc tcgtccccgc ggtgctgctg cccggcatgg cgctcgtgtt cctcatccat
      121 cccgacccgc gcgacatcgc caaggatctc ggcgcgtact acggcccgga cgtcgtgctc
      181 acgcaggccg ccgccgcgca cggaccgtcc gggaacggcg tgcgcgactg gctcgcgcac
      241 tacccactgc gtgtcgcctt cgtcgcgagc ttcgccgcgc agggcgtgat gtcgctcatg
      301 atggcgatga cctcgctcgc cctcgaccac cacggccacg ggctgcaggc gatctcgatc
      361 tcggtctcca tccacgtcgt cggcatgtac gcgctctcga tcccgctcgg gcatctcagc
      421 gatcgcttcg ggcgacgcaa cgtcatgctg gcgggaacgg tgctctcggc gctcggatcg
      481 atcctcgtcg gcgtggcgac cgaatacgcg ccgatcacga tcggcacgtt cctcgtcggt
      541 gtggggtggt cgtgcacgaa cgtgtcgacg tcagccctca tcgcggacgt cgtcgctccc
      601 gaggatcgtg gccgcgccat cggcacgtgc gacacgttca gcggcgcggg atcggtggcc
      661 ctgccgctcc tcggcggccc gctcgtggag atcgtcggct tcccgatcct cgcggtggtg
      721 gggacgctgc tcctcacgct cccgttcgcg atgctgctgc gtctccgcga gggcacgagg
      781 cgagagcacg ccgccgtggt cgcggaagcc gcggactgat ctggacctcc ctcctcgccc
      841 cgacggaggg ggtcgaggag gagtccagca ggaacgccgc gcggaccgcg ctagaagcgc
      901 tggtcgcgcg cggtgcgatc agcggcgcgc ttcgcgcagc acccgcagtg cgggtccggc
      961 cagacgagcc agaccacgag cgcgagccag ccggcgaaga ggccgatgct gaaggccata
     1021 tcagaagaga tcggcggcat aggccaccac ccgtggcaag ccgcgggcca acgccgccct
     1081 acgaggccgg gacgctcccg acggccgcga tggcctgggc caaggtgaac ttccccgcgt
     1141 agagcgcgcg cccgatgatc acgccgcgga tgccgcggtg gcgcaggcgc gcgagcgcgc
     1201 ggatgtcatc gagcgacccg acgccacctg agtagatgac gtcgccgccg aacgcccgtg
     1261 cgacggcctc cagcgacgcg agatcgacgc cctggagcgt cccgtcgacc gagacgttcg
     1321 tgtagatcac gatcggcacg ccctccacgg cgagccggtg catcagatcg acggccttcg
     1381 tcgtcgtcgt ctcggtccag ccggccgtcg tcaccatgcc gccccgcgcg tcgaccgcga
     1441 cagccatccc gtccgcatgg cggtcgacaa ggcgaccgat gagctcgggc tcctcgacgg
     1501 cggccgtgcc cacgacgatc cgagacgcgc cggcctccgc gaaccgctcc gccgtgtcga
     1561 aatcgcggat gccgcctccg gcctggatct tcaccgtgaa gcggttggcg atctgacgga
     1621 tggcgtcgag attcgcgggc gtccccgtgc gcgcgccgtc gaggtcgacg acgtgcaccc
     1681 actcggcgcc gtcgttcacc aggcggcgcg ccgcctcgag cggcgagggc gcgacctgct
     1741 cgagggtgtc gaagtcgcca cgcgtcatgc ggacgacgcg gccctcgtag atatcgatgg
     1801 ccgggtagac gatcacgcgg ccatggtacg cgcgggactt acacggacag cagcttctcg
     1861 aagaacgcgc ggtaccggcg gatcgcgacg cgcagctgct cggtgtcgag gcgctgcttg
     1921 gcgccgagct gctgccccag ccgatcgcgc tcgtcggtga agagcgcggc gagatcccga
     1981 aggacctcct cgacgagccc atcggccgcc ttcaccgccg ccttcgggtc gtcgacgaac
     2041 tgcgcctgca cgtcggtcca acgcgcgcgc agctcgtccg cgcgttcgga tgcgaacagt
     2101 ccgccaccgc tctcgtcctg cggctcccgt cgctcttcgc ggacctgctc gtcgcggacc
     2161 tggtcctcgc ggacctcttc gtcacgctcg ggcatatcga catcgcgtgt catcgcagct
     2221 gctcctctct ctcttcgacg cgctcaccgg ggcgggtccc agcaccgacc agctccgcga
     2281 acagcgcgcg gtagtgcacc atcgcgcggc ggagctcctc cgtcccggtc ttctgcgcgg
     2341 gccggcgcgc gatcgcgtgc gcctcgcggt agtcggacac gacctgcggg tgctcgacgg
     2401 aaacgtcggc ggcccgctgc tcgaagtcgc ccacggggta cccgcgggcc tccatcgcct
     2461 ggccaatgag gtcatcggcg tcggacacgg cgccacgcgg gtcgtcgacg aaccgcgcct
     2521 gcaccgcgcg ccaccgctca cggaacgccg ccgcgtcctg cgcgcgcagc ggccgaacat
     2581 gcagctgctc cacgcgctgt tgacgatcgt tcaagacacg ctccgcgcgg tcggcgtcgc
     2641 cctcctgccg cacgacgtgg tcgtactcgg gaccgaagcg ctcgcgaagc gcctcgcggc
     2701 gccgctgacg catcgcgtac cagatgaccg cgatcgcgac gaacgcgagg acgaggatcg
     2761 cggcgacgat cagctgctgc tggtccatca cgcacctccg ctaatggagg gtgacggcga
     2821 gcagatgccg tgccgttaca cgcgatccgt tacaggcgga aggcctttcg gccggcgtcg
     2881 tagccgcgtt ggtattgacc tatcgcggta aaggcgatga tcaatgtggc gagcgcggtg
     2941 ccgatgccga tccccacgat cagcgagatg acttccatgc gggacagagt ccttcacgcg
     3001 cgctcccgcc gacatcgggt ctgagacacc gcgctcgtac ggagccaccg cacgcgcggc
     3061 gggacgttcg acctaggtgg cgaggccgcg cagagcgtcg agcacgcggc gggtgctccc
     3121 ctcgatgccg tagcgcttgg caagcgcgtc gatgtcggcg cgcgggacac ccgagggaag
     3181 cgcgccactg atctcgggca cgtggcagtc gagcaccggc gagaccacgc gacgagcggc
     3241 gacgatgtag tcacggccgg cgttcagctt cgctttgatc gtcgccgaga gatccgtcgc
     3301 cgcgaggatg ccctcgagcg agccgtactt cgtgagcagc gccgcggcgg tcttctctcc
     3361 gatgccgcgc acgccgggca acccgtcgga cgggtcgccg cgcaggatcg cgtagtcgag
     3421 gtagcgatcg ccgggtatcc cgtacttggc gtgcacccac gcctggtcga catgcgcgag
     3481 ctcgctcacg cccttcaggg tgtagagcac cgtgatccgc gggtcgcgga cgagcgagta
     3541 gaggtcacga tcgccgctca cgacttcgac cgggccgtcg taccgcgtcg ccagcgtggc
     3601 gatgacgtcc tcggcctcgc actcacccgc gttcgccgtg gcgatgccga tggcccgcag
     3661 cacctcctcg atcacgacga tctgcggccc gacctcgtcc ggtggcgcgc cctcctccgc
     3721 gacccgatgg ctcttgtact cgggcagcgc ggtgacgcgg aactgcgggc gccagtcctc
     3781 tccgaactcg aacgccacga cacagcgcgc gggacggcga tccgtcagga ggcgcgcgac
     3841 gaagttgagg aagccgacga tcgcgttcac gagcgtgccg tccggcgcgt gcaccggcgg
     3901 catcgcgtgg tacgcgcgat acgccaggct cgacgcgtcg atgaggaggg tcgggcgatc
     3961 cgtggtcagg cgcgtaccgg cgccgggaag gcgtgcgcga gcgccgcgat gaagcggtcg
     4021 ttctc
//
LOCUS       DASTZR010000284         4003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_720566, whole genome shotgun sequence.
ACCESSION   DASTZR010000284 DASTZR010000000
VERSION     DASTZR010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720566"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..1341
                     /locus_tag="VFV20_06365"
     CDS             253..1341
                     /locus_tag="VFV20_06365"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU5288009.1"
                     /translation="MGRQRLLTLLSILGLVAVACGGTPTTASPTPTKAAATSAAATSA
                     APSAKPGELDDATIAKAKTEGEVVLYTSLSSSDAKKVGDAFAASAKSYGIKITVNRKS
                     SESLVTQFMTEVKAGKVLADVLETGGLDLFQAIKAGYIEPWKAPAAADFPADLKDANG
                     MWHAARLGVETISYNTTAVKEADAPKTFEDAADPKWKGKCLIEATDIEVLLGIGERKY
                     KGDTAKAQDLATKLIANCTPSNGHTETLDKLIAGEGAIFWGGHAHSTEQKRQDKAPVD
                     YMQAEGVVTIDGPGLVKGAAHPNAGRVFINWYLSDGQKVLADLFRVPAKPGAANPRLL
                     PKTIYYTGPTLAEKFATYQEIWTKTVKK"
     gene            1395..3053
                     /locus_tag="VFV20_06370"
     CDS             1395..3053
                     /locus_tag="VFV20_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU5288010.1"
                     /translation="MVIPALAALLLIMLTALPLGMLAIGSLQVEGTGGLTLANYLGVY
                     GDPRTYRLLQNSFVYAFGTSFVAIVLGTALAYIVERTNVPMRRAFFVVTLVPLIVPGI
                     VSTIAWIYLASGKIGVLNKMAMALFGLQSPPFDVYGMMGMIWTEGLHLSPLVFLVMVG
                     AMRAMDPSLEEAAVTSGARPLRTLRMITLPILVPALAASTLIMFVRGLESFEVPALLG
                     IPARIPVFTSEIYLALRQYPQDYGVAGALGMGLLAVSAVGVWTYGRMTRRAERFATIT
                     GKGYRPRRVDLGRWRFVALAFMAAYLLVVVVLPFLVLLFVSLLPFYIPSFDMLQRLTL
                     KNYATVLEHPDTLLAARNSLILALASATITMAITAVIAWIVIRTKLPGRRALDMLAFL
                     PIGIPGLVLAVAIVFELINFPIPIYGTLAILVIAYVTRYLPYGMRTNSAAMLQLHREL
                     EEAAAAAGATWSTTFRRIVVPLLWPSLVAGWIYVFVVSVRELSSSVLLVSPQSVVLSF
                     VIFDLQQSGQSTAVAALAVLLVVALVLIVGLTQGLSRRVGVREG"
     gene            3062..>4003
                     /locus_tag="VFV20_06375"
     CDS             3062..>4003
                     /locus_tag="VFV20_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5288011.1"
                     /translation="MLTVESLRKTYTTDRGGVRAVEDVSFTVEDGRFYTLLGPSGCGK
                     TTTLRSIAGLERPEGGTIRLDGDVLSGPGRFVPPYERDLGMVFQSYAIWPHMSVFDNV
                     AFPLRVGKHRPGSAEVKRRVEETLALVGLEGLEDRPAPQLSGGQQQRLALARALVRSP
                     KLLLLDEPLSNLDAKLRERMRIELRELQRRLRITTVYVTHDQGEALFLSHRIAVMHAG
                     RIVQEAGPRDIYAQPSSPFVADFVGLGTFLEGEAAPGGVRTLGSLVRCALPPDLVPGA
                     RALIVVRPESVRVHPSAPDLPNVFAGQLRVAAVLGDHL"
BASE COUNT          585 a         1413 c         1359 g          646 t
ORIGIN      
        1 gacgaagctg tccgagcggc gtctgtccgt gcccttgccg tcggcgagat cgcgataggc
       61 ggcctcgagg acggcgatgc agtcgggcat ggtcacgagg gcggcgacgt cgtcgttcga
      121 gaggaggagc gtcaccaggt cgtcccgcgc atcgcgcctt cctccttgac cggcccgccg
      181 cgcgtcacta ggctcgcgct caggtcgaaa ggtataacaa ttcgacatct ggacgggagc
      241 agggaggcac cgatgggcag gcagcgactt ctcacgttgc tcagcatcct cggcctggtc
      301 gccgtcgcgt gcggtggcac gccgacgaca gcaagtccga caccgacgaa ggctgcggcg
      361 acgagcgccg cggcgacgag cgcggcaccc agcgcgaagc ccggcgagct cgacgacgcc
      421 acgatcgcca aggcgaagac ggagggcgag gtcgtcctgt acacgtcgct ctcgtcgagc
      481 gacgcgaaga aggtcgggga cgccttcgcg gcgagcgcca agagctacgg catcaagatc
      541 accgtgaacc gcaagtccag cgagtcgctg gtgacgcagt tcatgaccga ggtgaaggcg
      601 gggaaggtgc tcgccgacgt cctcgagacc ggcggactcg atctgttcca ggcgatcaag
      661 gccggctaca tcgagccgtg gaaggcgccg gccgcggccg acttcccggc cgacctcaag
      721 gacgcgaacg gcatgtggca cgccgcgcgg ctcggcgtgg agacgatctc gtacaacacc
      781 acggcggtga aggaggccga cgccccgaag acgttcgagg acgccgccga cccgaagtgg
      841 aagggcaagt gcctcatcga ggcgaccgac atcgaggtgc tcctcggcat cggcgagcgc
      901 aagtacaagg gcgacaccgc caaggcccag gacctcgcga cgaagctcat cgcgaactgc
      961 acgccctcga acggccacac cgagacgctc gacaagctca tcgccggtga gggcgcgatc
     1021 ttctggggcg ggcacgccca cagcaccgag cagaagcgcc aggacaaggc gcctgtggac
     1081 tacatgcagg ccgagggcgt cgtgacgatc gacgggcccg gcctggtgaa gggcgccgcg
     1141 catccgaacg cgggccgcgt cttcatcaac tggtatctct ccgacgggca gaaggtcctc
     1201 gccgacctct tccgcgtccc ggcgaagccc ggtgccgcga acccgcggct ccttccgaag
     1261 accatctact acaccggccc gacgctggcc gagaaattcg cgacgtacca ggagatttgg
     1321 accaagaccg tcaagaagta gcgctcgcgg cgcggggggc gcgtttcgcg ctccccgcgc
     1381 tgcccagttc cggcgtggtg atacccgcgc tggcggcgct cctcttgatc atgctcacgg
     1441 cgctgccgct gggcatgctc gccatcggca gccttcaggt ggaaggcacg ggcggtctga
     1501 cgctcgcgaa ttacctgggc gtctacgggg acccgcgcac ctaccggctc ctgcagaact
     1561 ccttcgtcta cgcgttcggg acgtcgttcg tggcgatcgt gctcgggacc gcgctcgcct
     1621 acatcgtcga gcgcacgaac gtgccgatgc ggcgcgcgtt cttcgtggtc acgctcgtcc
     1681 cgctcatcgt ccccggcatc gtcagcacga tcgcctggat ctatctcgcg agcggcaaga
     1741 tcggcgtgct gaacaagatg gcgatggcgc tcttcggcct ccagagcccg cccttcgacg
     1801 tctacgggat gatgggcatg atctggacgg agggattgca tctgtcgccg ctcgtcttcc
     1861 tcgtgatggt cggcgcgatg cgcgcgatgg atccgtcgct cgaggaggcc gccgtcacga
     1921 gcggggctcg cccgctgcga acgctccgca tgatcacgct cccgatcctc gtgccggcgc
     1981 tcgccgcgtc gacgctcatc atgttcgtgc gaggcctcga gtcgttcgag gtgccggcgc
     2041 tcctcgggat cccggcgcgc atcccggtgt tcaccagcga gatctacctc gcgctgcgcc
     2101 agtacccgca ggactacggc gtcgcgggcg cgctcggcat gggcctgctg gcggtgagcg
     2161 cggtcggcgt ctggacgtac ggacggatga cgcggcgggc ggagcgcttc gcgacgatca
     2221 ccggaaaggg ctaccggccg cgccgcgtcg acctcggacg ctggcgcttc gtcgcgctcg
     2281 ccttcatggc cgcctatctc ctcgtcgtgg tcgtgctgcc gttcctggtc ctgctgttcg
     2341 tgtcgctcct gcccttctac atcccgagct tcgacatgct gcagcgcctc acgctgaaga
     2401 actacgccac cgtcctggag catcccgaca cgctgctcgc ggcgcgcaac agcctcatcc
     2461 tcgcgctcgc gagcgcgacc atcaccatgg ccatcaccgc cgtgatcgcg tggatcgtga
     2521 tccggacgaa gctccccggc cgccgcgcgc tcgacatgct cgcgttcctg ccgatcggca
     2581 ttccggggct cgtgctcgcc gtcgcgatcg tgttcgagct catcaacttc ccgatcccga
     2641 tctacgggac gctggcgatc ctggtcatcg cctacgtcac gcgctacctg ccatacggga
     2701 tgcgcacgaa ctccgcggcg atgctgcagc tgcaccgcga gctcgaggag gccgcggcgg
     2761 cggcgggcgc cacgtggtcc acgacgttcc ggcggatcgt cgtgccgctg ctgtggccgt
     2821 cgctcgtggc cggatggatc tacgtcttcg tcgtttcggt gcgcgagctg tcgtcgtcgg
     2881 tcctgctcgt ctcgccgcag agcgtcgtgc tctccttcgt catcttcgac ctgcagcagt
     2941 ccggccagtc gaccgcggtc gcggcgctcg cggtgctgct ggtcgtggcg ctggtcctca
     3001 tcgtcggtct cacccagggt ctgagccgtc gcgtcggtgt ccgggaggga tagggatgtc
     3061 cgtgctcacc gtcgaatcgc tgcgcaagac gtacacgacc gatcgcggcg gcgtgcgcgc
     3121 cgtcgaggac gtgtccttca ccgtcgagga cggccgcttc tacacactgc tcgggccctc
     3181 cggctgcggc aagaccacga cccttcgctc catcgcgggg ctcgagcggc ccgaaggggg
     3241 cacgatccgg ctggacggcg acgtgctctc cggtccggga cggttcgtac caccgtacga
     3301 gcgcgacctg ggcatggtct tccagtcgta cgccatctgg ccgcacatga gcgtcttcga
     3361 caacgtggcc ttcccgctgc gggtgggaaa gcaccgtccg gggtccgccg aggtgaagcg
     3421 ccgggtcgag gagacgctcg cgctcgtcgg cctcgagggg ctcgaggacc ggcccgcgcc
     3481 gcagctctcg ggcggccagc agcagcggct cgcgctcgcg cgcgcgctgg tgcgcagccc
     3541 gaagctgctg ctgctcgacg agccgctctc gaacctcgac gcgaagctgc gcgagcggat
     3601 gcgcatcgag ctccgcgagc tccagcgccg cctgcgcatc acgaccgtgt acgtgacgca
     3661 cgaccagggc gaggcgctgt tcctctcgca ccgcatcgcg gtcatgcacg cgggccgcat
     3721 cgtgcaggag gccggtccgc gcgacatcta cgcgcagccg tcctcgccgt tcgtcgcgga
     3781 cttcgtcggc ctcggcacgt tcctcgaggg ggaggccgcc cccggcggcg tgcggacgct
     3841 cggctctctg gtccgctgcg cgctgccacc ggatctcgtg cccggcgcgc gcgccctcat
     3901 cgtcgtgagg ccggagagcg tgcgcgtgca tccgtccgcg ccggacctgc cgaacgtctt
     3961 cgccggtcag ctccgtgtgg ccgcggtcct cggcgaccac ctc
//
LOCUS       DASTZR010000285         3944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_747353, whole genome shotgun sequence.
ACCESSION   DASTZR010000285 DASTZR010000000
VERSION     DASTZR010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747353"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VFV20_06380"
     CDS             complement(<1..888)
                     /locus_tag="VFV20_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018742048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU5288012.1"
                     /translation="MNVAPDVLALVGNTPLVRLSRIARGLRPTILAKMEHLNPGGSVK
                     DRIGLRMIEDAERRGLLRPGGTIVEPTSGNTGVGLALAAAIRGYRLVCTMADKQSQEK
                     RDLLRAFGAEVVVCPTAVPPESPESYYKVAERIARETNGFMPNQYYNAMNPESHYRTT
                     GPELWEQTDGRITHFVASVGTGGTISGAARVLKAKNPAIQVIGADPEGSVLSGDTARP
                     YLTEGVGEDFFPGTYDAAMVDRWVRVSDRDAFAMARRITREEGILSGGSCGTAMVAAR
                     EIVRELTAADGGSEAVVVVL"
     gene            complement(925..1362)
                     /locus_tag="VFV20_06385"
     CDS             complement(925..1362)
                     /locus_tag="VFV20_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288013.1"
                     /translation="MLIAVGAPFLLQAVGVPNASAYLFLALGLAFGAAWLMGARQYVY
                     LVPAASLITFGLGLVLPSWLSLNSDLAAPIFLGALAIGFFLVFVVEPHRPAPLIPAVL
                     LALVALLTLYGRGDLLPAWFQPMFVPLLFIALGAYLLVERRTD"
     gene            complement(1439..2653)
                     /locus_tag="VFV20_06390"
     CDS             complement(1439..2653)
                     /locus_tag="VFV20_06390"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014195868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU5288014.1"
                     /translation="MTSFATHLECARCHESYQLDVIQQTCPREGGPLLVRYDLRRVHG
                     AVEAEELRIRPATLWRYRELLPIDRDASIVTLGENNTPLLRTPALGAALGTLDLEIKD
                     EGLLPTGTFKARGAAVGVTRAKELGVSVIALPTAGNAGAAWAAYGARAGIRVVVVMPE
                     TTPQVIQRETAAYGADVWLVSGSIADAGAVVKKTCADRGWYDASTLKEPYRIEGKKTM
                     GFELAEQLGWRLPDVVIYPTGGGVGLIGMWKAFAELREIGWLPADATTRFVAAQATGC
                     APIVRAFEEGAAESQAWPDPKTFAAGIRVPKALGDFLVLRALRESGGTAVAVSEEEIA
                     AGMRAAGEHEGMLVCPEGGAALAAAARLHRDGWIRADERVVVFNTGSGLKYAESLQGR
                     SPRALAPGQLPT"
     gene            complement(2650..3927)
                     /gene="murA"
                     /locus_tag="VFV20_06395"
     CDS             complement(2650..3927)
                     /gene="murA"
                     /locus_tag="VFV20_06395"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU5288015.1"
                     /translation="MARFIVEGGVPLRGEITPSGNKNEALPLIASAMLTDEPTTLRNM
                     PRIRDVRGMLEIAAALGAKIEELDPRTVRISGGIRSTEVPRALAGEIRTSLLFAGPLL
                     ARAKKVTLGLPGGDVIGRRRNDTHFLALQALGAELDLSRDGYSLRTAGLRGAEVTLDE
                     ASVTATENALLAAVLASGRTVLNNAASEPHVQQLSHALVRMGAQIQGIGTNMLTIDGV
                     ERLSGVEHTILSDHMEVGSLIGAIAMTRGEVTIKNAVPRYLRMTRMVFKRLGVETDVA
                     GEDLVVKARDRYVIEEDLGGAIPTIKAQPWPGFPSDLSSIAIALATQAEGTVLVHEWM
                     FPGRMYWVGSLESMGARLVLCDPHRVVIVGPSQLYGSDLRSPDIRAGMALLNATLCAK
                     GKSVINNVEQIDRGYEALDERLRKLGAKIERAA"
BASE COUNT          587 a         1411 c         1381 g          565 t
ORIGIN      
        1 caacacgacg acgaccgcct cgctgccccc gtccgccgcg gtgagctcgc gaacgatctc
       61 gcgcgccgcg accatcgcag tcccgcacga accgcccgac aggatcccct cctcgcgcgt
      121 gatccggcgg gccatcgcga acgcgtcccg atcagagacg cgaacccagc gatcgaccat
      181 cgccgcgtcg taggtgccgg ggaagaagtc ctcgccgacg ccctcggtca ggtaggggcg
      241 ggcggtatcg ccgctcagca ccgacccctc gggatcggcg ccgatgacct ggatggccgg
      301 gttcttcgcc ttgagcacgc gggcagcccc ggagatcgtg ccacccgtcc cgacgctggc
      361 cacgaagtgg gtgatccggc cgtcggtctg ctcccacagc tccggaccgg tggtgcggta
      421 atgcgactcg gggttcatcg cgttgtagta ctggttcggc atgaagccgt tcgtctcgcg
      481 cgcgatccgc tccgcgacct tgtagtacga ctccggcgat tcgggcggga ccgcggtggg
      541 gcacacgacg acctcggcgc cgaacgcccg gagcaggtcg cgcttctcct ggctctgctt
      601 gtcggccatc gtgcagacga gccggtagcc ccggatcgcc gccgcgagcg cgaggccgac
      661 accggtgttg ccgctggtgg gttcgacgat cgtgccaccc ggacgcagca gcccgcggcg
      721 ttccgcgtcc tcgatcatcc ggagcccgat gcggtccttc accgacccgc cggggttgag
      781 gtgctccatc ttcgcgagga tcgtcggccg caggccgcga gcgatccgcg agaggcggac
      841 gagcggcgtg ttgccgacga gggccaggac atcaggcgcg acgttcacga cggtacagac
      901 gctactacgc gggggctggc agccctagtc ggtccggcgc tccaccagca gataggcacc
      961 gagcgcgatg aagagcagcg gcacgaacat cggctggaac cacgcgggca gcaggtcgcc
     1021 gcgaccgtag agcgtgagga gcgccaccag cgcgagcagg accgcgggga tgagcggcgc
     1081 cggacggtgc ggctcgacga cgaacacgag gaagaatccg atcgcgagcg cgccgaggaa
     1141 gatcggcgcc gcgagatcgc tgttcaggct gagccacgac gggagcacca ggccgaggcc
     1201 gaatgtgatg agggaggcgg ccggtacgag atacacgtac tggcgcgcgc ccatgagcca
     1261 cgcggcgccg aacgcgagac cgagcgccag gaacaggtat gcgctggcgt tcggcacgcc
     1321 gacggcctgc agcaggaacg gcgctccgac ggcgatgagc accgacccac cgaggattcc
     1381 tcgacgtccg tgtccacgtg cttgcatggt cgagcggcag tctagcgggc cgaccgcgct
     1441 aggtcggtag ctgtccgggc gcgagcgcgc gcggtgagcg gccctggagc gactccgcgt
     1501 acttcagacc gctgcccgtg ttgaacacga cgacccgctc atctgcgcgg atccagccgt
     1561 cccgatgcag ccgcgcggcg gccgcgagcg cggccccacc ctcgggacac acgagcatgc
     1621 cctcgtgctc gccggcggcg cgcatgcccg cggcgatctc ctcctcggac accgccacgg
     1681 ccgtgccgcc actctcgcgg agcgcgcgca gcacgaggaa gtcgccgagc gccttgggga
     1741 cgcggatgcc cgcggcgaac gtcttcgggt cgggccaggc ctgcgactcg gctgcgccct
     1801 cctcgaaggc gcgcacgatc ggcgcgcatc cggtggcctg cgccgcgacg aagcgcgtgg
     1861 tggcatccgc gggcagccag ccgatctcgc gcagctcggc gaaggccttc cacatgccga
     1921 tgaggccgac gccgcccccg gtcggataga tcacgacgtc cggcagccgc catccgagct
     1981 gctcggcgag ctcgaacccc atcgtcttct tgccctcgat gcggtacggc tccttcagcg
     2041 tggatgcgtc gtaccagccg cggtcggcgc acgtcttctt caccacggca cccgcgtcgg
     2101 cgatggagcc ggacacgagc cacacgtcgg cgccgtacgc cgcggtttcg cgctggatga
     2161 cctgcggtgt cgtctcgggc atcacgacga cgacccggat gccggcgcgc gcgccgtacg
     2221 cggcccaggc cgcgcccgcg ttccccgcgg tcggaagcgc gatcacgctc acgccgagct
     2281 ccttcgcgcg cgtgacgccg accgcggcgc cacgcgcctt gaacgtgccg gtcgggagca
     2341 ggccctcgtc cttgatctcg aggtcaaggg tccccagcgc cgcgccgagc gccggcgtcc
     2401 ggaggagcgg cgtgttgttc tcgccgagcg tcacgatgct cgcgtcgcga tcgatcggca
     2461 gcagctcgcg gtagcgccac agcgtggctg gccggatgcg cagctcctcg gcctcgacgg
     2521 ctccgtgcac gcggcggagg tcgtagcgga cgagcagcgg gcccccctcg cgcgggcagg
     2581 tctgctggat cacgtcgagc tggtacgact cgtgacagcg ggcgcactcc agatgggtcg
     2641 cgaacgacgt cacgccgcgc gttcgatctt cgcgccgagc ttccgcaggc gctcgtcgag
     2701 cgcctcgtac ccgcggtcga tctgctcgac gttgttgatg acgctctttc ccttggcaca
     2761 cagcgtcgcg ttgaggagcg ccatgcccgc acggatgtcg gggctgcgca gatcggagcc
     2821 gtacagctgc gacggcccga cgatcaccac gcgatgcgga tcgcagagca cgagccgcgc
     2881 acccatgctc tcgagcgaac ccacccagta catgcggccc gggaacatcc actcgtgcac
     2941 gagcaccgtg ccctccgcct gcgtcgcgag cgcgatcgcg atgctcgaga gatccgatgg
     3001 gaagccgggc cacggctgcg ccttgatggt ggggatggcg ccgccgagat cctcctcgat
     3061 gacgtagcga tccctcgcct tcacgacgag gtcctcgccg gcgacatccg tttcgacgcc
     3121 gagccgcttg aacaccatgc gcgtcatgcg caggtaccgc gggacggcgt tcttgatcgt
     3181 cacctcgccg cgcgtcatcg cgatggcgcc gatgagcgag cctacctcca tgtggtcact
     3241 gaggatcgtg tgctcgacgc cgctcagccg ctcgacgccg tcgatcgtca gcatgttcgt
     3301 gccgatgccc tggatctgcg cgcccatgcg cacgagcgcg tggctgagct gctgcacgtg
     3361 cggctcgctc gcggcgttgt tgagcaccgt ccgtccgctc gcgagcacgg ccgcgaggag
     3421 cgcgttctcg gtcgccgtca ccgacgcctc gtcgagcgtc acctcggcac cacgcagacc
     3481 ggcggtccgc agcgagtacc cgtcgcggct gaggtcgagc tccgccccga gcgcttggag
     3541 ggcgaggaag tgcgtgtcgt tgcggcggcg gccgatgacg tcgccacccg gcagtccgag
     3601 cgtgaccttc ttcgcgcgcg cgaggagcgg acccgcgaac agcagtgagg tgcggatctc
     3661 gccggcaaga gcgcgcggca cctcggtcga gcggatgccg ccgctgatgc gcacggtgcg
     3721 cggatcgagc tcctcgatct tcgcgccgag cgcggcggcg atctcgagca tgccgcggac
     3781 gtcgcggatg cgcggcatgt tgcggagcgt cgtcggctca tcggtgagca tcgcggacgc
     3841 gatgagcgga agcgcctcgt tcttgttccc ggacggcgtg atctcgccac gcagtgggac
     3901 gccgccctcg acgatgaaac gcgccatcgg cggcctagcc tagc
//
LOCUS       DASTZR010000286         7439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_821826, whole genome shotgun sequence.
ACCESSION   DASTZR010000286 DASTZR010000000
VERSION     DASTZR010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7439
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821826"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..522
                     /locus_tag="VFV20_06400"
     CDS             46..522
                     /locus_tag="VFV20_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288016.1"
                     /translation="MIKERAQGLLSMLRANAARAKDLDLDDLKGTLRDLDWKSFRDVQ
                     LRGAKDAVEGIRHMRRDPAAQRRNGVMLAAVGVAIGAALMYLFDPRNGSSRRSSVRER
                     VKGWYLSGRGRLDRDRREARAGGHHVDPVENARTDGQPLDTPADASRLEATALSTS"
     gene            679..1842
                     /locus_tag="VFV20_06405"
     CDS             679..1842
                     /locus_tag="VFV20_06405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5288017.1"
                     /translation="MDVRQTVLLVEDDLDLSKLVSGLIAEAGYQPITIADHAQISSAV
                     ERWQPRCVILDGEVRSTGEGRSWDDAVALRRTHPTLPVLMFTADTAALAEAESGTSAR
                     SRAAGFTGSVSKPFLVEEFLATLRAAMPESARPDSPSGSRSDPGRAEAISVFPDLTGR
                     YATEAERGDLVASAAHELRAPLTVMRGQMQLARRRLAHDPVRSGMALDVAIAQVDRMA
                     RTIDEVIEHSRLAANALALNVVPFDLLDLVAQAIERHQHSDSMRIRLERTDATSAPCR
                     GDPGRIAEILDNLIDNALKYSAADAPVELTVTAANGEAQVRVQDHGVGVPDDEVVGLF
                     APYYRTTRTRHVAGTGLGLHISRQLAERNRGRLWLESSSSAGSSFVLALPLAR"
     gene            complement(1920..2621)
                     /locus_tag="VFV20_06410"
     CDS             complement(1920..2621)
                     /locus_tag="VFV20_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5288018.1"
                     /translation="MQLIRVLSVSADQALARSIDLTLSRRRYVHRSEGTAQAAKAAIS
                     GWRPHLVLLDLDIEAGAGIQLIDEARSCVRIGVIALTRRGDLHQQLDVYARGIDDYVR
                     VPFVREDLAARIGAVLRRTHEDAGVPRVLFGDLEIDVLNRRVLTGERELHLTSLEQAL
                     LYLLAANAGDVLSRDQILDALWGADCAVESNSVERHVRALRAKLHDDWREPRYIETVR
                     GVGYRLVPAPDAVPC"
     gene            complement(2871..3269)
                     /locus_tag="VFV20_06415"
     CDS             complement(2871..3269)
                     /locus_tag="VFV20_06415"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEU5288019.1"
                     /translation="MTTTTTPIEPTSKPAATNVNVSTNTSPSANTSLWTASRVIALFF
                     SVLEVLLLVRFALKLFGANAEQALVSAIYGVTEPLVAPFRGIFAQPAGTPVVEIATLL
                     SIVFFVLVAALAVAAVRAATGRRNESNPST"
     gene            complement(3302..3484)
                     /locus_tag="VFV20_06420"
     CDS             complement(3302..3484)
                     /locus_tag="VFV20_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288020.1"
                     /translation="MPDLWFFIGLAMGIALGGFASVGSYARGADSVRNAAWRRDLPLR
                     QLVARASRGSRHADAA"
     gene            3589..4152
                     /locus_tag="VFV20_06425"
     CDS             3589..4152
                     /locus_tag="VFV20_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288021.1"
                     /translation="MTDDRVTTLMLQEFRLSAVDTDGAVARIVSELPAGLEPAVPLLT
                     SIDDPHDVAMIRVLRDGESDGLDVIERAGVDPFISSWQPTRHYDARIVESSQSPPTHY
                     RLAVTESGINDEARAPSSARPRGGALNEASASVSLLWIGRPHGSHGGLLILLGDDGDP
                     EAARSDPHEWPLPMSRGLGVRIYDSAA"
     gene            4162..4905
                     /locus_tag="VFV20_06430"
     CDS             4162..4905
                     /locus_tag="VFV20_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5288022.1"
                     /translation="MPHTAASRQPDRSIRVLIAVRDQALAKTIDLTLRHGSYERRSEG
                     TVKDARATLVQWKPHILLLDIDLEQGAGIQLIDEARAGGPVGIIALTRRSDLRGKLDA
                     FERGADDYIGIPFVPDDLVARTRAVIRRTNGNPGQLVPSLRIGDLEIDVLNRKVLAGD
                     HELHLTSLEQALLYLLAANAGSVLTREQILDALWGTDFVIESNVVDRHVRALRAKLQN
                     DWHKPRYIETVPGAGYRFVAAEEEPDRIV"
     gene            5042..6136
                     /locus_tag="VFV20_06435"
     CDS             5042..6136
                     /locus_tag="VFV20_06435"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5288023.1"
                     /translation="MSDAQKVLIVEDDLTVSELVADVLIDAGYRPLAIADHALIAETV
                     ERFRPRCVLLDGELTRAGESRSWHDAAAIRRAHPTLPVVLFTGDATVAAEARAARSYR
                     SRAAGFAGIIGKPFVIEDLLATVRAAVDTSSDTDTQVFSVIVHELRQPLAVIRGQVEL
                     ALRQHGADPEGERLALDRSLTQVRRMDVLIDQLLDHARVAADGFSLEVTLVDLASAVA
                     DVIATRQYGAMPRISFVGPSGAVPVYGDPARIVQIVENLLSNALKYSAAGTPIEVSLT
                     TDGVEAEVRVADHGVGVPEEERALLFAPFYRTSRARDVHGTGLGLHISRRLAEHHGGR
                     LWLDQSSSAGSVFAFRIPVARGDPMPARSS"
     gene            complement(6133..6438)
                     /locus_tag="VFV20_06440"
     CDS             complement(6133..6438)
                     /locus_tag="VFV20_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003386422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5288024.1"
                     /translation="MSELARALDIVGHKWTLLIVQDLLGGPRRFTEIQRSLAGANPKM
                     VTARLRELEAARLISRTSYAEVPPRVEYALTERGRALRPAVDALRRWGAGAPPRRPR"
     gene            6515..6961
                     /locus_tag="VFV20_06445"
     CDS             6515..6961
                     /locus_tag="VFV20_06445"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEU5288025.1"
                     /translation="MTLVQDKPTVATNIATIKQWVAAMAKGDLDHAPYAEDAEMSDPT
                     GKYKGKAQIVQSFKVWNTAFPQGTAEVINQIAQGEQVASEVVYKATHTGPLVMSAVTI
                     PPTSKPIELKVMLIQSFRDGLIQRERAYFDKAGLMQQLGVTVPPKP"
     gene            complement(7016..7171)
                     /locus_tag="VFV20_06450"
     CDS             complement(7016..7171)
                     /locus_tag="VFV20_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288026.1"
                     /translation="MRKPIIGAVVAIAAIAAVRRFGPDLKERAKAKCHAMMSAPAAAP
                     CCAAAAA"
     gene            complement(7168..7425)
                     /locus_tag="VFV20_06455"
     CDS             complement(7168..7425)
                     /locus_tag="VFV20_06455"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEU5288027.1"
                     /translation="MIKKNVGRVDREIRQSVGAVLAILGLVVLGGLQGQAAGLAVSVL
                     ALLGLLTGDTGRCPLYQLLGISTLGASERDGAEATREEAAA"
BASE COUNT         1230 a         2582 c         2495 g         1132 t
ORIGIN      
        1 cgcggtccgc gggatggcgg cgcgacgcgt tctcggaggt gactcgtgat caaggaacgc
       61 gcacaggggc tgctctccat gctcagagcg aacgcggcac gggcgaagga tctggacctc
      121 gacgacctca aggggacgct cagggatctc gactggaaat ccttccgcga cgttcagctt
      181 cgcggcgcca aggacgctgt cgagggcatc aggcacatgc gccgcgatcc cgcagcacag
      241 cgtcgcaacg gcgtgatgct cgcggcagtc ggcgtggcga tcggtgccgc cctgatgtat
      301 ctcttcgatc cacggaatgg cagttcgcgc cgctcgtcgg tgcgcgaacg cgtcaagggc
      361 tggtacctga gcggtcgcgg ccggctggac cgcgaccggc gcgaagcgcg cgccggcggt
      421 caccacgtcg atcccgtcga gaacgcgcgg acggacgggc aacccctcga cacaccagcc
      481 gatgcgtcgc gcctggaagc gaccgcgctc agcacgtcct agcgaccccg cgcttccctc
      541 ccacgctgtt gggaatcggg acagtggtct gtggccctga cggaaccgga gtgacggttc
      601 ggtcatgtca caaacccacg tcagcgccac gatgccagga gcaggggagg gtcgctgtgc
      661 cgatcgcggg aggacctcat ggatgttcga cagacggtgc tgctggtcga ggatgacctc
      721 gacctgagca agctcgtcag cggcctcatc gccgaggccg gatatcagcc gatcacgatc
      781 gccgaccatg cgcagatctc gtccgcggtc gagcgatggc agccgcgctg cgtgatcctc
      841 gacggcgagg tgaggtcgac cggcgagggc cgctcgtggg acgacgcggt cgccctgcgc
      901 cgcacccatc cgacgcttcc cgtcctcatg ttcaccgccg acaccgcggc gctcgccgag
      961 gcagagtcgg gcacgtcggc gcgcagccgc gccgccggct tcaccggaag cgtgagcaag
     1021 ccgttcctcg tcgaggaatt cctcgccacg ctccgcgcgg cgatgccgga gtcggcccgg
     1081 ccggactcgc catcgggcag ccgctccgat cccggccgcg cggaggccat cagcgtgttc
     1141 ccggatctca ccgggcggta tgccaccgag gcggagcgtg gcgacctggt cgcctcggcg
     1201 gcgcatgagc tgcgcgcgcc gctcacggtc atgcgcggtc agatgcagct cgcccgccga
     1261 cgcctcgcac acgacccggt gcgcagtggc atggccctcg atgtcgcgat cgcgcaggtc
     1321 gatcggatgg cgcgcaccat cgacgaggtc atcgagcact ctcggctggc cgcgaacgcg
     1381 ttggccctca acgtcgttcc tttcgatctt ctcgacctcg tcgcacaggc gatcgagcgt
     1441 caccagcact cggactcgat gcgcattcgc ctcgagcgca cggacgcgac gagcgcgccg
     1501 tgccgcggag accccggtcg catcgcggag atcctcgaca acctcatcga caacgctctg
     1561 aagtacagcg cggcggacgc gccggtcgag ctgacggtga cggccgcgaa cggtgaggca
     1621 caggtgcgtg tgcaggacca tggtgtcggc gtgccggacg acgaggtcgt cggcttgttc
     1681 gccccgtact accgcaccac ccggacgcgt cacgttgcgg ggaccggtct cgggctgcac
     1741 atcagccggc agctcgctga acgcaaccgt ggacggctgt ggctcgaatc gagctcgagc
     1801 gcgggaagct cgttcgtgct ggccttgccg ctggcgcgct gaacggactc acgctcgccc
     1861 gttcgcccgt gatcctcgtc ggcgggttcg ctctcctcta acgctcttcc ctgcggcggt
     1921 cagcacggaa ccgcgtcagg agcagggacg agtcgatagc cgaccccgcg caccgtctcg
     1981 atgtagcgag gctcgcgcca atcatcgtgg agcttcgcgc gaagagctcg cacgtgccgc
     2041 tcgacgctgt tgctttcgac ggcgcagtcc gcgccccaga gcgcatcgag gatctgatcg
     2101 cggctgagca cgtccccggc gttcgcggca aggaggtaca gcagcgcctg ctcgagcgag
     2161 gtgaggtgca gctctcgctc tccggtgagc accctgcggt tgaggacgtc gatctcgagg
     2221 tccccgaaga ggacacgcgg gacccccgca tcctcgtgcg tccgccgcag caccgcgccg
     2281 atgcgggcgg caaggtcctc gcgcacgaat gggacccgta cgtagtcgtc gatgccgcgc
     2341 gcgtagacgt cgagctgctg gtgcaggtcg ccgcgccgag tcagcgcgat gacaccgatc
     2401 cgcacgcacg atcgtgcctc gtcgatgagc tggatcccgg cgccggcctc gatgtcgagg
     2461 tcgagcagga cgagatgcgg ccgccacccc gagatcgccg ccttcgcagc ttgcgccgtc
     2521 ccctcggatc ggtggacata ccgcctgcgt gagagcgtca ggtcgatgct tcttgccagc
     2581 gcctggtccg ccgacacgga cagcactcgg atgagttgca tgtcgtcgcg ctccctcggt
     2641 ctcccttcgg cgagcatgac gcgcgagcaa gcgagccttg agagtcgaag gtcacgtatc
     2701 gcggggactg gcgggcatcg gccccgtgcg gttatcgcga catttccagt cggacacaca
     2761 ccgcgagcgc acccaattga gaatgggctc agggagccag ccgcctcgtg acgcgcgatg
     2821 cgccccgatc cttcgatcgc ggcgcatccg caagactcga ccgggtatgt ctaggtggag
     2881 gggttcgact cgttgcgcct gcccgtggcc gcgcgtaccg cggcaacggc gagagccgcg
     2941 acgagcacga agaagacgat cgagaggagc gtcgcgatct cgacgaccgg cgtgccggcg
     3001 ggttgcgcga agatgccgcg gaacggtgcg acgagcggtt cggtcacgcc gtagatcgct
     3061 gagacgagcg cctgctccgc gttcgctccg aagagcttca gcgcaaagcg gaccaggagg
     3121 agcacctcga gcacgctgaa gaagagcgcg atcacccggc tcgccgtcca gagcgatgtg
     3181 ttcgccgatg gcgatgtgtt cgtggatacg ttcacgttgg tcgccgcagg cttggatgtc
     3241 ggttcgatcg gtgtggtggt cgttgtcatg tgaatgcctc catgcatgtg gtgcgaaggc
     3301 gtcacgccgc gtcggcgtgg cgcgagccgc gagatgcgcg cgctaccagc tgacgcagcg
     3361 gaaggtcgcg acgccaggcc gcgttccgca cactgtcggc gccgcgcgcg tacgagccga
     3421 ccgaagcgaa tcccccgagc gcgatgccca tggcgagacc gatgaagaac cacagatctg
     3481 gcatggatcg agcctaggcg cgaggcgggc gcttggcatg acgcaaccgt cacctcctcg
     3541 tgacggttag gtcatgcgtc gcgtacccgg acgcgcgact ctgaacgcat gaccgacgac
     3601 cgcgtcacga cactcatgct ccaagagttc cggctctcag ccgtggacac cgacggcgcc
     3661 gtcgcgcgca tcgtgagcga gctgccggca ggcctcgagc ccgccgtgcc gctcctgacc
     3721 agcatcgacg acccgcacga cgtggcgatg atcagagtgc tacgtgacgg tgagtccgac
     3781 gggctggacg tgatcgagcg cgccggtgtc gacccgttca tctcgagctg gcaaccgacc
     3841 aggcattacg acgcacggat cgtcgagagc tctcaaagcc cgccgacgca ctaccgactc
     3901 gcggtgacgg aatcgggcat caacgacgag gcacgcgccc cgtcttcggc acggccacgc
     3961 ggaggggctc tcaacgaggc gtccgcatcc gtcagtctcc tgtggatcgg ccgaccacac
     4021 gggtcgcatg gcggtctgct catcctcctc ggcgacgacg gcgatcccga ggcggccaga
     4081 tccgatccac acgagtggcc ccttcccatg agccgcggcc tgggcgtccg catctacgac
     4141 agcgcggcct agacttcctt cgtgcctcac accgccgcgt cgcggcaacc cgaccgcagc
     4201 atccgagtgc tcatcgccgt gcgagaccag gcgctcgcga agaccatcga cctcacgctg
     4261 cgccacggca gctatgagcg ccgatccgag gggacggtga aggatgcgcg ggccacgctc
     4321 gtccagtgga agcctcatat cctgctcctc gacatcgatc tcgaacaggg cgcggggatc
     4381 cagctcatcg acgaggcgcg cgcgggaggg ccggtcggga tcatcgcgct cacgcggcgg
     4441 agcgacctgc gtgggaagct cgacgcgttc gagcgcggtg cggacgacta catcgggatc
     4501 cccttcgtcc cggacgacct cgtcgcgcgt acgcgtgcgg tgatccgccg gaccaatggg
     4561 aaccccggac aactcgtacc cagcctgcgc atcggcgatc tcgagatcga cgtgctgaac
     4621 cgcaaggtgc tcgccgggga ccacgagctg catctcacct ccctcgagca ggcgctgctg
     4681 taccttctcg ccgcgaatgc cggcagcgtc ctcacgcgcg agcagatcct cgatgccctc
     4741 tggggcacgg atttcgtcat cgagagcaac gtcgtggacc ggcacgtccg cgcacttcgc
     4801 gcgaagctgc agaacgactg gcacaagccg aggtacatcg agaccgtgcc cggggccgga
     4861 taccggttcg tcgccgccga agaggagccc gaccgcatcg tctgatccgc gcgttttcgc
     4921 ccgatttccg gcctcgcgtg ggggagttac caaaccgtca tgcggcgccc gaggcgttag
     4981 cggacgatcg gtggcgctgg ccggcgtgtg cgctcgccgt cggccggccc ggggagggcc
     5041 agtgtcggac gcgcagaagg tactcatcgt cgaggatgac ctgacggtca gtgagctcgt
     5101 cgcggacgtc ctcatcgatg ccggctaccg cccgctcgcc atcgccgatc acgcactcat
     5161 cgcggagacg gtcgagcgct tccgcccgcg ctgcgtactc ctcgacggcg agctgacgcg
     5221 agcaggcgag agccgctcat ggcacgacgc ggccgcgatc cgccgggcgc atccaacgct
     5281 gccggtggtc ctgttcaccg gtgatgccac cgtcgccgcc gaggcacggg ctgcgcgttc
     5341 gtaccgcagc cgtgccgcag gctttgccgg gatcatcggg aagcccttcg tgatcgagga
     5401 ccttctcgcc accgtaagag ctgccgtcga cacgtcctcc gacaccgata cccaggtgtt
     5461 cagcgtgatc gtccacgaac tgcggcagcc cctcgccgtc atccgcggcc aggtagagct
     5521 cgccctccgc cagcacggag cggaccccga gggagagcgt ctggcgctcg accgcagcct
     5581 cacgcaggtg cgccggatgg acgtcctgat cgaccagctg ctggaccacg cgcgtgttgc
     5641 cgccgacggc ttcagcctcg aggtgaccct ggtcgacctg gccagtgcgg tggcggatgt
     5701 gatcgccacg cgccaatacg gtgcgatgcc gcgcatcagc ttcgtggggc cgagcggtgc
     5761 cgtaccggtc tacggcgacc cggcgcgcat cgtgcagatc gtggagaacc tgctcagcaa
     5821 cgcgctcaag tacagcgccg ccgggacgcc gatcgaggtg tcactcacga cggacggcgt
     5881 cgaggcggag gtccgcgtcg ccgatcacgg ggtcggcgtt cccgaagagg aacgcgccct
     5941 tctcttcgcg cccttctacc gcacgtcgcg ggcgcgcgat gttcacggca cgggactcgg
     6001 cctgcacatc agcaggaggc tcgccgagca ccacggaggc cggctctggc tggaccaaag
     6061 ctccagcgcg ggcagcgtct tcgcgttcag gatccccgtc gcccgcggcg atcccatgcc
     6121 ggcgcgaagc agctagcgcg gccgtcgggg cggcgcaccg gcgccccatc tccgcagcgc
     6181 atcgacggcc ggacgcagcg cgcggccgcg ctcggtgagc gcgtattcca cgcgtggcgg
     6241 tacctcggcg tagctcgtcc gcgagatgag ccgtgcggcc tcgagctccc gcaagcgtgc
     6301 ggtgaccatc ttcgggttgg cgccggcgag cgagcgctgg atctcggtga acctgcgtgg
     6361 accgcccagc agatcctgga cgatgagcag cgtccacttg tgcccgacga tgtccaaggc
     6421 acgcgccaat tcgctcacaa tacttacgtt atgggatgta acttgcttat tgcaagtatc
     6481 acgagtacgg tggacggaga aagaagggca atctatgaca ttggtacaag acaaaccgac
     6541 agtcgcgacc aacatcgcga cgatcaagca gtgggtcgcc gccatggcca agggcgacct
     6601 cgatcacgct ccgtacgccg aggacgccga gatgtccgac ccgacgggca agtacaaggg
     6661 caaggcgcag atcgtccagt ccttcaaggt ctggaacacc gccttcccgc agggcaccgc
     6721 cgaagtgatc aaccagatcg cccagggcga gcaggtcgcg tcggaggtcg tctacaaggc
     6781 gacgcacacc ggcccgctcg tcatgtcggc cgtgaccatc ccgccgacga gcaagccgat
     6841 cgagctcaag gtcatgctca tccagtcgtt ccgcgatggt ctgatccagc gcgagcgcgc
     6901 ctacttcgac aaggccgggc tcatgcagca gctcggcgtg accgtcccgc cgaagccgta
     6961 aggcttctcc gcggacactt cgaaggagca cccgagaggg tgctccttcg tcgtcctacg
     7021 cagccgctgc cgcgcagcag ggcgcggcgg ccggggcgga catcatcgcg tggcacttcg
     7081 ccttcgcgcg ttccttcaga tccggtccga agcggcggac cgctgcgatc gcggcgatcg
     7141 ccacgaccgc accgatgatc ggcttcctca tgccgctgcc tcctctctcg tcgcttcagc
     7201 gccgtcgcgc tcgctcgcac cgagcgtgct gatgccgagc agctggtaca gcgggcaacg
     7261 gcccgtgtcg ccggtcagca gaccgaggag cgcgagcacg gacacggcca gacctgcggc
     7321 ctgcccctgc agccctccga gcacgacgag gccgaggatc gccagcaccg cgccgaccga
     7381 ctgacggatc tcgcggtcga cgcggcccac gttcttcttg atcatcgaag atcacctcc
//
LOCUS       DASTZR010000287         3363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1191157, whole genome shotgun sequence.
ACCESSION   DASTZR010000287 DASTZR010000000
VERSION     DASTZR010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191157"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1628)
                     /locus_tag="VFV20_06460"
     CDS             complement(<1..1628)
                     /locus_tag="VFV20_06460"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU5288028.1"
                     /translation="MREVADRPRVTAKDARETLEPMLDDLARGLGYDRALVLAHDEHT
                     ASLRGLFGMNIRDEQAQLLVVPLSNGRDPMVTALRTGAPQRVDDAKVDERLDEQERAL
                     LTSLRLGSFVAAALPGMGDERPSAVVVLARDRGIGEEDLDRLLPFARQAGALMTREHD
                     AELLRRASESHAIEKEWLWWMLNSVADPVLVADARNDIIQLNRRAEMLFRASDEDSAG
                     KRRAVWMNNFLFTAALSTWSLDQPSRSTNREVTLVDPIDGSELIFEVITRSALNHRTG
                     ERGTVSVLKDVTDIRHMTVELTRSVQRIQTADEEIRAERDRLDLVLRSVPNPIIVVDN
                     DNQIISMNAAAQRLFGHQGKVQHALANDAKFTSFLAQLRLDPAPERRSELALIDPTTE
                     ERLEMEITAAEVRDTHGAVVAIVSAMQEIGRLRELERRRLEHVLFETEKLAATGRLAA
                     SIAHEINNPLEAVQNSLYLLATALPADAPERRYMEIAQRETQRRSRILRQMLGFYKPT
                     RDMAPIDVNALIEEVSVLIAKRLREGKVNVERVLA"
     gene            complement(1725..1919)
                     /gene="gvpJ"
                     /locus_tag="VFV20_06465"
                     /pseudo
     CDS             complement(1725..1919)
                     /gene="gvpJ"
                     /locus_tag="VFV20_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175973.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0012506 - vesicle membrane [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpJ"
     gene            complement(1923..2231)
                     /locus_tag="VFV20_06470"
     CDS             complement(1923..2231)
                     /locus_tag="VFV20_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011429713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein"
                     /protein_id="HEU5288029.1"
                     /translation="MTIERRLRDTESSASLVDLLDRVLDKGLVVAGDISISLANVELL
                     TIRVRLLVCSIDKAEQIGLNWWRYESRLSRRDGDGDDEVKRLEARIAELERQLKQKGG
                     "
     gene            complement(2228..2518)
                     /locus_tag="VFV20_06475"
     CDS             complement(2228..2518)
                     /locus_tag="VFV20_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein K"
                     /protein_id="HEU5288030.1"
                     /translation="MRRELARVSAVLPERIDLDPDDVERGLAGLVLGLVELLRQVLEK
                     QAVRRMEGGTLTDEEIERVGLALMRLERKVRELADHFGLSEEDIQLRLGAAS"
     gene            complement(2551..3192)
                     /locus_tag="VFV20_06480"
     CDS             complement(2551..3192)
                     /locus_tag="VFV20_06480"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031411 - gas vesicle [Evidence IEA];
                     GO_process: GO:0031412 - gas vesicle organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HEU5288031.1"
                     /translation="MTLLVRSFVRASDADRVGIPGLVVVRAGGVAAIATETDAPPTAT
                     EDALRAHVAMTGAIHDAAPSLPARFGSIFAGRDELAQALASREAALLGALDAVGDRIE
                     MSVTLSWRTARAGGTADASSGRAYMEALGTRERERERAEQITARLVGELGYERAFTRE
                     SICPRGGVAATVALLIPRDEVMRVRRHVASFRDRTSEVMVAAYGPLAPYSFAS"
     gene            complement(3189..3356)
                     /gene="gvpJ"
                     /locus_tag="VFV20_06485"
     CDS             complement(3189..3356)
                     /gene="gvpJ"
                     /locus_tag="VFV20_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0012506 - vesicle membrane [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpJ"
                     /protein_id="HEU5288032.1"
                     /translation="MPLVDIIDRLLGRGVVLAGGAVISVAGVDLIELRLSVVLASVDA
                     FDRLREPAETT"
BASE COUNT          502 a         1174 c         1150 g          537 t
ORIGIN      
        1 gcgagcaccc gctcgacgtt caccttgccc tcgcgcagcc gcttcgcgat cagcacgctg
       61 acctcctcga tcagcgcgtt cacgtcgatc ggcgccatat ccctcgtcgg cttgtagaag
      121 ccgagcatct ggcggaggat gcgtgacctc cgctgcgtct cgcgttgcgc gatctccatg
      181 tagcggcgct ccggggcgtc ggcgggcagg gccgtcgcca gcaggtagag cgagttctgg
      241 acggcctcga gggggttgtt gatctcgtgc gcgatcgatg cggcgagccg gcccgtcgcc
      301 gcgagcttct cggtctcgaa gagcacgtgc tccagccggc ggcgctcgag ctcgcgcagc
      361 cgtccgatct cctgcatcgc cgacacgatg gccacgaccg cgccgtgcgt gtcgcgcacc
      421 tcggccgcgg tgatctccat ctcgagccgc tcctcggtgg tcggatcgat gagcgcgagc
      481 tcggagcggc gctcgggtgc gggatcgagg cgcagctgcg cgaggaacga cgtgaacttc
      541 gcgtcgttcg cgagcgcgtg ctgcaccttg ccctggtggc cgaagagccg ctgcgccgcg
      601 gcgttcatgc tgatgatctg gttgtcgttg tccacgacga tgatcgggtt gggcacgctg
      661 cggaggacga gatcgagccg gtcgcgctcg gcgcggatct cctcgtcggc ggtctggatg
      721 cgctgcacgc tgcgcgtgag ctcgacggtc atgtggcgga tgtcggtgac gtccttgagg
      781 acggacacgg tgccgcgctc accggtgcgg tgattcagcg cgctgcgcgt gatgacctcg
      841 aagatgagct cgctgccatc gatcggatcg accagcgtca cctcgcggtt cgtcgagcgg
      901 ctcggctggt caaggctcca tgtcgagagg gccgcggtga agaggaagtt gttcatccac
      961 acggcgcgcc gcttgccggc gctgtcctca tccgaggcgc ggaagagcat ctcggcgcgc
     1021 cggttgagct gaatgatgtc gttgcgggcg tccgcgacga ggacgggatc ggcgaccgaa
     1081 ttcagcatcc accagagcca ttccttctcg atcgcgtgcg actccgatgc gcgacgcagc
     1141 agctccgcgt cgtgctcccg cgtcatcagc gcgccggcct ggcgtgcgaa gggcaggagc
     1201 cggtcgaggt cctcctcgcc gatgccccgg tcgcgtgcga ggacgacgac ggcgctcggc
     1261 cgctcgtcgc ccatgccggg cagcgccgcg gcgacgaacg acccgagcct cagcgaggtg
     1321 aggagcgcgc gttcctgttc atcgaggcgc tcgtcgacct tcgcgtcatc gacgcgctgc
     1381 ggcgcgccgg tacgcagggc cgtgaccatc ggatcgcgcc cgttcgacag gggcacgacg
     1441 agcagctgcg cctgctcgtc ccggatgttc ataccgaaga gcccgcgcag cgatgcggtg
     1501 tgctcgtcgt gcgccagcac gagcgcgcgg tcgtacccga gcccacgcgc gagatcgtcg
     1561 agcatcggct cgagcgtctc tcgcgcgtcc ttcgccgtca ccctggggcg gtcggcgacc
     1621 tcccgcatgc ggggccgcga gcgctcttgg gcctgtcgcg accgggtcgt cgccgtatcg
     1681 atgccgccgg cacccgtacc gagggtgccc tgccgcgcgc ccggcgtgag gccgagcgcg
     1741 tccgcgtagc gcagatacgt gtcgatggag gccacgacca cgcgtgcctc gacggtgatg
     1801 aggtcgatgc cgacgagcga gatgcggacc caggcatcga tgatgatgcc cttgtcgagg
     1861 acgcgatcga ggacatcgat gaggctgctg ccaccagggc tccgttcaac gaccgccatg
     1921 actcatcccc ctttttgctt cagttgtcgc tcgagttcgg cgatccgcgc ctcgaggcgc
     1981 ttcacctcat cgtcgccatc gccatcgcgc cgcgagagcc gcgattcgta acgccaccag
     2041 ttcaggccga tctgctcggc cttgtcgatc gagcagacca ggagccgcac gcgaatggtc
     2101 agcagctcga cgttcgcgag cgagatcgag atatcgcccg cgacgacgag acccttgtcg
     2161 agcacgcggt ccaacagatc gacgagcgat gcggagctct ccgtatcgcg aagacgccgc
     2221 tcgatcgtca tgacgccgct ccgagccgca actgtatgtc ctcttcgctc agtccgaagt
     2281 ggtccgcgag ctcgcgcacc ttccgttcga gcctcatgag cgcgagaccc acgcgttcga
     2341 tctcctcatc ggtcaatgtg ccaccctcca tccgccgtac cgcctgcttt tcgaggacct
     2401 gccgcaggag ctcgacgagg ccgagcacca ggccggcgag cccacgctcc acgtcgtcgg
     2461 ggtcgaggtc gatgcgttcg ggcagcaccg cgctgacccg cgccagctcg cggcgcacct
     2521 catcgagctc gagttgaaga tcggtgttcg tcacgatgcg aacgagtacg gcgccagggg
     2581 gccgtaggcg gcgaccatga cctcgctcgt ccggtcgcgg aacgacgcga cgtgtcggcg
     2641 cactctcatc acctcatccc gcgggatcaa cagcgcgact gtggcggcaa caccgccgcg
     2701 cgggcaaata gattcacggg tgaacgctcg ctcgtatcca agctcaccga ccagacgagc
     2761 ggtgatctgc tcagctcgtt cccgctcgcg ctctcttgtg ccgagcgcct ccatgtacgc
     2821 acgtccggac gacgcgtcgg ccgtgccgcc ggcccgagcc gtccgccacg acagcgtgac
     2881 cgacatctcg atccggtcgc ccaccgcgtc cagcgcgccg agcagcgccg cctctcgcga
     2941 cgcgagcgcc tgggcgagct catcgcggcc cgcgaagatc gagccgaagc gcgccggcag
     3001 ggatggcgcg gcgtcgtgga tggccccggt catggcgacg tgcgcgcgga gggcgtcctc
     3061 ggtggccgtc ggcggcgcat cggtctcggt cgcgatcgcg gccacgccgc cggcgcggac
     3121 gaccacgagc ccgggtatgc ccacccggtc ggcgtcggac gcgcggacga aggaacgaac
     3181 gagcagcgtc acgtcgtctc cgccggctcg cgcaggcggt cgaacgcgtc caccgacgcg
     3241 agcacgacgc tcagacgaag ctcgatgagg tccacgccgg cgacggagat gaccgcgcca
     3301 ccggcgagca ccacgccacg cccgagaagg cggtcgatga tgtctacgag cgggatctcg
     3361 gtc
//
LOCUS       DASTZR010000288         2078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1113134, whole genome shotgun sequence.
ACCESSION   DASTZR010000288 DASTZR010000000
VERSION     DASTZR010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113134"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="VFV20_06490"
     CDS             <1..942
                     /locus_tag="VFV20_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288033.1"
                     /translation="GDRWVQRAVEAGRLVRIRAGIHADAREWDAAGHDHRALVRTAAA
                     VGTRRQDAVLTHESAALVLGIPIIGARPDAVHLASSTGTAPRSERGVVWHRERLRDAD
                     LVEYGGFVLTSPERTAFDLARTRGFASAVTGIDAVLAGRFEPAPVRFDGDRLVAWEPD
                     VFAAVDRTALAGRIAESAGARGIRSARRVVEFSDPRSASPGESLSRAQIHLLGFPPPE
                     LQVAFERRDGGADVTDFLWPDFAIAGEFDGSVKYHREEYLNGMPPEEVVWLEKERERR
                     LRRDHGLEVSRWVWDVARTPHLLRSELVAAGLPVVRR"
     gene            1225..1617
                     /gene="rpsF"
                     /locus_tag="VFV20_06495"
     CDS             1225..1617
                     /gene="rpsF"
                     /locus_tag="VFV20_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011187104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU5288034.1"
                     /translation="MHQYELMVILDPSIDERTVAPSLDKFLNVIRNDGGSIDNIDIWG
                     KRRLAYEIDKKSEGIYAVVNFTAESATTNELDRQLNLSEAVMRTKVLRSEEAVARAEK
                     IQAADAKRAAKKATATANKAAAAAKKDV"
     gene            1623..>2078
                     /locus_tag="VFV20_06500"
     CDS             1623..>2078
                     /locus_tag="VFV20_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU5288035.1"
                     /translation="MAGETIITVVGNLTADPELRYTQNGLAVANFTIASTPRTFDRQA
                     NEWKDGEALFLRASCWREFAEHVAGSLTKGTRVIASGRLKQRSYETKEGEKRTTIELE
                     VDEIGPSLRYATASVTRAQSNRGVGGGGSFGGGGGQSDDAWAPSAPAQSS"
BASE COUNT          321 a          720 c          728 g          309 t
ORIGIN      
        1 ggtgaccgtt gggtgcagcg ggcggtggag gcgggtcggc tcgtgcgcat ccgggcgggc
       61 attcatgccg acgctcgcga gtgggacgcg gccggtcacg accatcgagc actcgtgcgc
      121 accgccgcgg ccgtcggaac ccgccgtcag gatgccgtgc tcacgcacga gtccgccgcg
      181 ctggtcctcg gcatcccgat catcggggct cgtcctgacg ccgtgcacct cgcctcgtcg
      241 acgggtactg cgccccggag cgagcgcggc gtggtgtggc atcgcgagcg actccgcgac
      301 gcggatctgg tcgagtacgg cggtttcgtg ctcacgagtc ccgagcggac cgccttcgac
      361 ctcgcccgca cacgcgggtt cgcctcggcg gtcaccggga tcgatgccgt gctggccggg
      421 cgcttcgagc cggcgccggt gcgcttcgac ggtgaccgac tcgtcgcctg ggagccggat
      481 gtcttcgctg cggtggaccg gaccgcgctc gccgggcgga tcgccgagtc ggcgggtgcg
      541 cgcggcatcc gctcggcgcg acgtgtcgtc gagttctccg atccacgatc cgcatcgccg
      601 ggtgagtcgc tcagtcgcgc gcagatccac ctgctcggct tcccgccgcc cgaactgcag
      661 gtcgcgttcg agcgtcgaga cggcggggcg gatgtcaccg acttcctctg gccggacttc
      721 gcgatcgccg gcgagttcga cggatccgtg aagtatcacc gcgaggagta cctgaacggg
      781 atgcctccgg aggaagtggt gtggctcgag aaggagcgcg aacgccgact tcgccgcgac
      841 cacgggctcg aggtgagtcg gtgggtgtgg gatgtcgcgc gaaccccgca tctgctgagg
      901 tccgagttgg tcgcggccgg gctcccggtc gtgcgccgct gacgcactac cgcgcggttc
      961 cgacgccgag cgcgtgcaga actgacgaac gcaattgcat ccgcgtgcgc actcgtcgga
     1021 agtgcacgcg ctcggcgaac ctcagggcgg ggcggggatg ggtgcgtgga acccgctcgc
     1081 gccgatgtgc gcgcgggagc ggcatccgtt atgatctgaa ggttgcccgt gcgccgcagt
     1141 cgcggcctcc tcggagcgac gcatgtacac cctcctgtca cagaccgtct gtgaccgcct
     1201 gagtccgaag gaggtgggtc tgtcatgcac cagtacgagc tgatggtcat cctcgatccg
     1261 tcgatcgacg agcgcaccgt tgctcccagc ctcgacaagt tcctcaacgt catccgcaac
     1321 gatggcggtt cgatcgacaa catcgacatc tggggcaagc gtcgcctggc ctacgagatc
     1381 gacaagaagt ccgagggcat ctacgccgtc gtgaacttca ccgccgagtc ggcgacgacc
     1441 aacgagctgg accgccagct gaacctcagc gaggccgtca tgcgcacgaa ggtcctccgc
     1501 tccgaggagg ccgtcgcccg cgccgagaag atccaggccg ccgacgccaa gcgcgccgcc
     1561 aagaaggcga ccgccaccgc gaacaaggcc gccgccgctg ccaagaagga cgtctagtcc
     1621 tcatggccgg cgagaccatc atcaccgtgg tgggcaacct caccgccgat cccgagctgc
     1681 gctacacgca gaacgggctc gcggtcgcga acttcacgat cgcgtccacg ccccgcacgt
     1741 tcgaccgcca ggcgaacgag tggaaggacg gcgaggccct cttcctgcgc gcgagctgct
     1801 ggcgcgagtt cgccgagcac gtcgcgggat cgctcaccaa gggaacccgg gtcatcgctt
     1861 ccgggcgtct caagcagcgt tcctacgaga cgaaggaagg cgagaagcgc accaccatcg
     1921 agctcgaggt cgacgagatc ggcccgagcc tgcgctacgc caccgcgtcc gtgacgcgcg
     1981 cccagtccaa ccgcggcgtc ggcggcggcg gctccttcgg gggcggcggc ggccagtcgg
     2041 atgacgcgtg ggcgccgagc gctcccgcgc agtcgtcg
//
LOCUS       DASTZR010000289         3274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_434199, whole genome shotgun sequence.
ACCESSION   DASTZR010000289 DASTZR010000000
VERSION     DASTZR010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434199"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="VFV20_06505"
     CDS             complement(<1..852)
                     /locus_tag="VFV20_06505"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5288036.1"
                     /translation="MTRIVAVGCGWVTLHRHIPAIRGTVGLELAGVVDRDAARARDSG
                     TRLRVPHATSLDDPALGAFDAVSIGTAPMAHHGVAMRALALGKHVITEKPFAMTVAEA
                     DEMIAEADKRGRILAVVHNFQFARSVAAARRIAESGKYGRLRGVIGLQLSNERRRLPV
                     WYRTLPGGLFYDEGPHLIYLVRAFLGDVEVAHASAIRSGDPDDATPRLVEASFETGAG
                     GPFGALQMSFAAALSEWQLVVLLERATLVCDIFRDILVRFPDDGLHVPGQILRTSFAG
                     SLGHWRGV"
     gene            879..1742
                     /locus_tag="VFV20_06510"
     CDS             879..1742
                     /locus_tag="VFV20_06510"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5288037.1"
                     /translation="MRHPFSRDDILRLLAPSNVYQPATMLWRVFEIEAIQRHATLAGR
                     GVDIGCGDGELAKVVFAASEPRPTLVGVEPDERDCASARASGTYAAVHCVGGDAVPEP
                     DASFDFAFSNSTLEHIPVIEPVLREAARLIRSGGTFLFTVPSDEFHRCLHGNRFLERR
                     ARGRGMAYTALIDQRLAHHRYLGPDGWTALLRDAGFSDALYERYFPRAAVRTWEWLSN
                     LTGGLLFEVFGARRATRQIQHGLGLSRRSAPALARVVETVTRRAARAALAAEVRPGEP
                     SGGLLVIATRG"
     gene            complement(1748..2875)
                     /locus_tag="VFV20_06515"
     CDS             complement(1748..2875)
                     /locus_tag="VFV20_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002787625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5288038.1"
                     /translation="MPCLNEAKTVGTCVEKAKRALASLGIAGEVIVADNGSTDGSREL
                     AAGAGARVVPVSARGYGSALLGGIAAARGRYVLMGDADDTYDFGAIAPFVDKLRAGFD
                     LVMGNRFTGGIEPAAMPALHRYVGNPVLSFLARLFFGGSVGDFHSGMRAFRTDAIRAL
                     DLRTTGMEFASEMVVKASLHGLSITEVPVRLYPDRRGRPPHLRTWRDGWRHLRFLLLY
                     SPRWLFLTPGIVLMVIGASVGLWLLGGPRTAGPIGFDVHTLIYASGAMIIGFQLVLFA
                     LFTKIFAISEGLLPEDQRLNRLFRYITLETGLVVGTALAVVGLVGSLYAVGIWQSRSF
                     GAIEETSRTLRIVITSVTALAVGMQVIFASFFLSILGMRRG"
     gene            complement(2902..>3274)
                     /locus_tag="VFV20_06520"
     CDS             complement(2902..>3274)
                     /locus_tag="VFV20_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288039.1"
                     /translation="YPSRADAPSTGSARGCERGGNLYEEIRPSRLEFVVECANDAALA
                     IKVTYHPNWSVRVDGQLAETFMLSPSYLGVQLAAGRHSVVAEYRSTPGKAPLLAAGLL
                     GLVGACVGGTLLDRRRGWWSR"
BASE COUNT          526 a         1145 c         1138 g          465 t
ORIGIN      
        1 gacgccgcgc caatgaccga gcgatcccgc gaaggacgtg cgcaggatct gacccggcac
       61 gtgcagcccg tcgtcaggga accgaacgag gatgtcgcgg aagatgtcgc acacgagcgt
      121 ggcacgctcc aggagcacga cgagctgcca ctccgagagc gcggcggcga agctcatctg
      181 gagggcaccg aagggaccgc ccgcgcccgt ctcgaagctg gcctcgacga gccgcggcgt
      241 cgcgtcgtcc ggatcgccgc tgcggatcgc gctcgcgtgc gcgacctcga catcgcccaa
      301 gaaagcgcgc accaggtaga tgaggtgagg tccttcgtcg tagaacaggc cgcccggcag
      361 cgtgcggtac cagacgggaa gacgccggcg ctcattcgac agctggaggc cgatgacccc
      421 gcgcagccgt ccgtacttcc cgctttccgc gatccggcgc gccgcggcca cgcttcgtgc
      481 gaattggaag ttgtggacga cggcaaggat ccgtccgcgc ttgtcggcct ccgcgatcat
      541 ctcgtccgcc tcagcgaccg tcatcgcgaa cggcttctcg gtgatcacgt gcttgcccag
      601 cgcgagcgcg cgcatcgcca cgccgtgatg cgccatggga gcggtcccga tgctcaccgc
      661 gtcgaacgcg ccgagggcgg gatcgtcgag actcgtcgcg tgtggtaccc gcaggcgcgt
      721 gccggaatct cgcgctcgcg cggcatcgcg atcgacgacg cccgcgagct cgagtcccac
      781 cgtccctcgg atcgcgggga tatgccggtg gagcgtgacc cagccacatc cgacggcgac
      841 gatgcgcgtc acgggcggcc tctatgctgc cgactggcat gcgtcacccg ttttcgcgcg
      901 acgacatcct ccgtctgctc gcgccatcca acgtctacca gccggcgacg atgctctggc
      961 gcgtgttcga gatcgaggcc atccagcggc acgccacgct cgcggggcgc ggtgtcgaca
     1021 tcggttgcgg cgatggcgag ctcgcgaagg tcgtcttcgc cgcgtccgag cctcgaccca
     1081 cgctcgtcgg tgtggagccg gacgagcgcg actgcgcttc ggcccgggcg tctggcacat
     1141 acgccgcggt ccattgcgtc ggcggcgatg cggtgccgga accagacgcg tcgttcgact
     1201 tcgcgttctc caactcgacg ctcgagcaca ttccggtgat cgaacccgtg ctgcgggagg
     1261 ccgcgcgcct gatccgctcc ggcgggacgt tcctgttcac cgtgccgagc gacgaattcc
     1321 atcgctgcct gcacggcaac cgctttctcg agcgacgcgc gcgcggccgt ggcatggcgt
     1381 acacggccct catcgatcag aggctcgcgc accaccgcta tctcggaccc gacggttgga
     1441 cggcgctcct tcgcgacgcg ggcttcagtg atgcgctgta cgagcgctac ttcccgcgcg
     1501 cggcggtgcg gacctgggag tggctctcga atctcacggg gggactgctc ttcgaggtct
     1561 tcggcgcgcg ccgcgcgacg cgccagatcc agcacggtct cggtctgtcg cggcgctccg
     1621 cgccggccct cgcccgcgtc gtcgagacgg tcacgcgtcg cgccgcacgt gcggcgctcg
     1681 ccgccgaggt gcggccggga gagccctcgg gcgggttgct cgtcatcgcg acacgcgggt
     1741 aggaacgtca tccgcgtcgc atgcccagga tgctgaggaa gaagctcgcg aagatgacct
     1801 gcatgccgac ggcgagcgcg gtgacggagg tgatgacgat ccgtagcgtg cgcgacgttt
     1861 cctcgatcgc gccaaacgac cgcgattgcc agatcccgac ggcatagagc gagcccacga
     1921 gcccgacgac cgccagggcg gtgcccacga cgagcccggt ctcgagggtg atgtagcgga
     1981 acagacgatt gagacgctgg tcctctggca ggaggccctc gctgatggcg aagatcttcg
     2041 tgaacagcgc gaagagcacc agctggaagc cgatgatcat cgcgcccgac gcgtagatca
     2101 gcgtgtggac gtcgaatccg atgggacccg ccgtgcgtgg accgccgagc agccatagcc
     2161 ccaccgaggc gccgatgacc atgaggacga tgcccggcgt gaggaagagc caacgcgggc
     2221 tgtagagaag gaggaagcgc agatgacgcc agccgtcgcg ccaggtgcgc aggtgcggcg
     2281 ggcgaccacg acggtccgga tagaggcgca ccgggacctc ggtgatcgag agcccgtgga
     2341 ggctcgcctt cacgaccatc tcgctcgcga actccatacc ggtggtgcgc aggtcgagcg
     2401 cgcggatcgc gtcggtgcgg aaggcgcgca tcccggaatg gaagtcgccg acgctcccgc
     2461 cgaagaagag gcgcgccagg aacgagagca ccgggttgcc gacgtagcgg tgcagcgccg
     2521 gcatcgccgc cggctcgata cccccggtga agcgattgcc catgacgagg tcgaacccgg
     2581 cgcgcagctt gtcgacgaag ggtgcgatcg cgccgaagtc gtaggtgtca tccgcgtcgc
     2641 ccatcagcac atagcggccg cgcgcggcgg cgataccacc caagagcgcg ctgccgtatc
     2701 cgcgtgccga gaccggcacc acccgcgcgc ccgcgccggc ggcgagctcg cgcgagccgt
     2761 cggtgctgcc gttgtccgcg acgatgacct cgcccgcgat cccgagcgac gcgagtgcgc
     2821 gcttggcctt ctcgacgcag gtgcccacgg tcttcgcctc gttgaggcac ggcatcacga
     2881 ccgagacctc cagctccgcg gtcaccgcga ccaccatccc cgccggcgat cgagcaaggt
     2941 gccgcccacg caggcgccga cgagtccgag gagccccgcg gccaagagcg gcgccttccc
     3001 gggcgtcgag cggtactccg cgacgaccga gtggcggccg gcggcgagct gcacgcccag
     3061 atagctggga ctcagcatga acgtctcagc gagctggccg tcgacacgta cggaccagtt
     3121 tgggtgatac gtcaccttga tcgcgagcgc tgcgtcgttc gcgcactcga cgacgaactc
     3181 gagacggctc ggcctgatct cctcgtagag attcccgccg cgctcgcagc cgcgcgccga
     3241 ccccgtcgag ggcgcgtcgg cgcgcgaggg gtag
//
LOCUS       DASTZR010000290         3234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1243652, whole genome shotgun sequence.
ACCESSION   DASTZR010000290 DASTZR010000000
VERSION     DASTZR010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243652"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..683)
                     /locus_tag="VFV20_06525"
     CDS             complement(15..683)
                     /locus_tag="VFV20_06525"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEU5288040.1"
                     /translation="MSAARRAMLVARRELDAERHQPDGVLSALTFAGLLVLLESLAFG
                     PGRARDADVASAVFWIAMLFAAVLVVARSFDRELEDDAIDAIIALDGGRDALYAGKVL
                     ALAAIVAVIAVAAGAFSLFLLDLDVALAAHLVLVTLLGVVALPPVVVLVSLIALRVRA
                     RVVIVPILAVPLLVPQLLAATAGASAALSGDAGGALGWSGLLASFALVYGVLGLTIVP
                     AAIE"
     gene            complement(680..1213)
                     /locus_tag="VFV20_06530"
     CDS             complement(680..1213)
                     /locus_tag="VFV20_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5288041.1"
                     /translation="MSLEIVPGLTVIRGPNGAGKTTLLRAIAGLVPLARGSRTVSAST
                     LFIGHRPMLLRGLSARQNLTFMTEFRGHAAVDVPSALARWGVAPVMDRPVERISAGQR
                     RRASLARIETEPEPIVLLDEPFADLDHAGVELLRSAIAAAVGGGRAIVLVTHLHDELD
                     REAARRVVIADGEVRAA"
     gene            1264..1635
                     /locus_tag="VFV20_06535"
     CDS             1264..1635
                     /locus_tag="VFV20_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU5288042.1"
                     /translation="MRKGALAVILAVAALASSCAPEPRATEPVARGRQVYGELGCGSC
                     HEAGLMNLWRPVGPPLDHVGTVAAGRRQGVPAGEYLRQSIVEPGSYVVPGYPDSMPRG
                     LGDRISPEDLADLVQYLSTLR"
     gene            1638..2231
                     /locus_tag="VFV20_06540"
     CDS             1638..2231
                     /locus_tag="VFV20_06540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5288043.1"
                     /translation="MAPNDGAQAPVTPVGQDEDKALAIRASGGDVSAFGLLYDRHVDA
                     IYRYVYYRVRDDAEAEDLTSDVFMRALKAMPRYEPRQAFLAWLYRIARNAVIDRARRG
                     NRQVSFEDALEHPTADQIVEPDVELLAHADNDTLRKALAKLTPLQQEVVVLRFLEGYS
                     THEIARLVGKREGTIRGIQFRAIGALRQLIPTREALG"
     gene            2228..3028
                     /locus_tag="VFV20_06545"
     CDS             2228..3028
                     /locus_tag="VFV20_06545"
                     /inference="COORDINATES: protein motif:HMM:NF039823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HEU5288044.1"
                     /translation="MSRLPEPRMRDEFRAELRNKLILEAQTALAPRPRGTAWTALLRH
                     SLTRPALAAMVAAIVLLAGTGSAAAGSLPGDPGFGLKRAIEDLQVSLTFDDVQRVQLL
                     AQLTDRRLAELQQVADHADRAPTASEEYAEAVARFRAAVDALQQAAPREKADRAQDVA
                     DAAREKHDAIIEDLAPRVNEKAKDALERAQEEEHRDNPSRTKDPRRTDRPERSRSPEP
                     SGSPRPSETFRSSETPRATERADSTRTPRPSGTATPRPSARETDGERD"
     gene            complement(3045..>3234)
                     /locus_tag="VFV20_06550"
     CDS             complement(3045..>3234)
                     /locus_tag="VFV20_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288045.1"
                     /translation="PMDAVHRLMRRVPTAIAGVVRWSLATVDDDTPRNVIPMPGPAHG
                     PVPSITLRVEEQRRRKAS"
BASE COUNT          519 a         1158 c         1170 g          387 t
ORIGIN      
        1 cctgctgcca gatttcattc gatcgcggca ggaacgatgg taaggccgag cactccgtac
       61 acaagcgcga aggaagcgag cagccccgac catcccagcg cgccgccggc gtcgcccgac
      121 agcgccgcgg acgcaccggc cgtcgccgcg aggagctgcg gcacgaggag cgggaccgcg
      181 aggatcggga cgatcacgac gcgtgctcgc acgcgcagcg cgatgaggct cacgagcacg
      241 acgaccggcg gcagcgcgac gacgccgagc agcgtgacga gcacgaggtg cgccgcgagc
      301 gcgacgtcga ggtcgagcag gaacagcgag aaggcgccgg ccgccaccgc gatcaccgcg
      361 acgatcgcgg cgagcgcgag cacttttccc gcatagagcg cgtcgcgtcc accgtcgagc
      421 gcgatgatcg cgtcgatcgc gtcgtcctcg agctcccggt cgaacgagcg ggcgacgacg
      481 aggacggcgg cgaagagcat cgcgatccag aacacggcgc tcgcgacgtc ggcgtctcgt
      541 gcacggccgg gaccgaaggc gaggctctcg aggaggacga ggaggccggc gaaggtcagc
      601 gcggacagga cgccgtccgg ctggtgacgc tcggcgtcga gctctcggcg tgccacgagc
      661 atggcgcggc gtgcggcgct catgcggcgc gcacctcgcc gtcggcgatg acgacgcggc
      721 gcgcggcctc gcgatcgagc tcgtcgtgca ggtgcgtcac gagcacgatc gcgcggccgc
      781 cgccgacggc ggcggcgatt gcgctccgca ggagctcgac accggcgtga tcgagatccg
      841 cgaacggctc gtcgagcagc acgatcggct cgggctccgt ctcgatgcgc gccagcgacg
      901 cgcggcgccg ctgtcccgcc gagatgcgct cgaccggccg gtccatcacg ggagccacgc
      961 cccaccgcgc gagcgccgac ggcacgtcga cggcggcgtg gccgcgaaac tcggtcatga
     1021 aggtgaggtt ctggcgcgcg gagagcccgc gaagcagcat gggcctgtgc ccgatgaaca
     1081 acgtgctcgc actgaccgtc cgcgagcccc gcgcgagcgg gacgaggccc gcgatcgcgc
     1141 gtaacagcgt cgtcttgccc gcgccattgg gaccgcggat taccgttagg cccgggacga
     1201 tctccaggga caccccgcgt aacaccgcgc ggtcgtcgag gacgaccgtg gcatcgtgaa
     1261 gggatgcgga agggagcact ggccgtcatt ctcgccgtcg cggcgcttgc cagctcctgc
     1321 gcccccgagc cacgcgccac cgagccggtc gcccgcgggc ggcaggtcta cggggagctg
     1381 ggctgcggct cctgccacga ggccggcctg atgaacctct ggcggccggt cgggcccccg
     1441 ctcgaccatg tcggaacggt cgcagccggc aggcggcagg gggtcccggc cggcgaatac
     1501 ctgcgccagt cgatcgtcga accgggctcg tacgtagtac cggggtaccc agactcgatg
     1561 ccgcgggggc tgggcgaccg gatttcgccc gaagacctgg ccgatctggt ccagtacctc
     1621 tcgacgttac ggtgaagatg gctcctaacg acggcgcgca ggcacccgtt acccccgtag
     1681 ggcaggacga ggacaaggca ctcgccatcc gggcctccgg gggcgatgtc tcggccttcg
     1741 gcctcctcta tgaccggcac gtcgacgcga tctaccggta cgtctactac cgagttcgcg
     1801 acgacgccga agcggaggat ctgacgagcg acgtgttcat gcgagcgctg aaggcgatgc
     1861 cgcgatacga gccgcgtcag gcattcctcg cctggctcta tcggatcgcg cgcaacgcgg
     1921 tgatcgatcg ggcgcgccgt ggcaaccgcc aggtgtcgtt cgaggacgcc ctcgagcacc
     1981 cgacggccga tcagatcgtc gagcccgacg tcgagctgct cgcgcacgcg gacaacgaca
     2041 cgctgcgcaa ggcgctcgcg aagctgacgc ccctgcagca ggaggtcgtc gtcctccgat
     2101 tcctcgaggg atacagcacg cacgagatcg cccgcctcgt cggcaagcgc gaaggcacca
     2161 ttcgtgggat ccagttccgc gccatcggcg cgctgcgtca gctcatcccg acgcgggagg
     2221 cgctcggatg agccgtcttc ccgagccgcg catgcgcgac gagttccgtg cggagctgcg
     2281 gaacaagctg atcctggagg cgcagaccgc gctcgcgccg cggccgcgcg gcacggcgtg
     2341 gacggcgctc ctgcgtcact cgctcacgcg ccccgcgctc gccgccatgg tcgccgcgat
     2401 cgtcctgctc gccggtacgg gctcggcggc cgcgggcagc ctgccgggag acccgggctt
     2461 cggcctgaag cgcgccatcg aggatctcca ggtcagcctc acgttcgacg acgtccagcg
     2521 cgtccagctg ctcgcgcagc tcaccgaccg tcgtctcgcg gagctccagc aggtcgcgga
     2581 tcacgccgac agggcgccga ccgcgtcgga ggagtacgcc gaggcggtcg cgcggttccg
     2641 tgccgcggtc gatgcgctcc agcaggcggc gccgcgcgag aaggcggaca gggcgcagga
     2701 cgtcgccgac gccgcgcgcg agaagcacga cgcgatcatc gaggacctcg cgccgcgcgt
     2761 gaacgagaag gcgaaggacg cgctcgagcg cgcgcaggaa gaagagcacc gcgacaatcc
     2821 gagccggacc aaggatccga ggcgcaccga tcgtccggag cgctcgcgtt cgcccgaacc
     2881 gagcggctcg ccgcggccgt cggagacctt ccgctcatcc gagacgccgc gcgcgaccga
     2941 gcgggccgac agcacgagga cgcctcgccc gagcgggacg gccacgccgc gcccgtccgc
     3001 gcgcgagacc gacggggagc gcgactagcc ccgacccgcg ctcctcacga cgccttgcgg
     3061 cggcgctgct cctcgacgcg gagtgtgatc gacggcacgg gtccgtgtgc cgggcccggc
     3121 atcgggatca cgttgcgcgg ggtgtcatcg tcgaccgtgg cgaggctcca gcgcaccacg
     3181 cccgcgatcg ccgtcgggac gcggcgcatg agccggtgaa cagcatccat cgga
//
LOCUS       DASTZR010000291         2653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_956607, whole genome shotgun sequence.
ACCESSION   DASTZR010000291 DASTZR010000000
VERSION     DASTZR010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956607"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..583
                     /locus_tag="VFV20_06555"
     CDS             50..583
                     /locus_tag="VFV20_06555"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018040026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEU5288046.1"
                     /translation="MATATKQLNGLDLEAMGTLVQTVTKDPSAADPLNDWSARVRWES
                     GFRSKALVRNHTFTVDEPATLIGEDLAPNAAEYVLAAYGACLTTGMALNATKNGVKLR
                     NVEVVVEGSLDNILTFFGMSDRGHPGFREISVKAYIDADTDDATLNRIWRETVASSPV
                     GNTCVRNVVLRDELVRV"
     gene            576..1757
                     /locus_tag="VFV20_06560"
     CDS             576..1757
                     /locus_tag="VFV20_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018193362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEU5288047.1"
                     /translation="MSRELPLFPVTTVGSWPRPASLIRALKDKNAGAIDQAEFEAAAD
                     AAVLEILRVQEDAGVDVVTDGEQRRDNFYSFLADKLDGVRLMTMAELIDYAEDKAFFH
                     RTLRSLDAPSFVIKSPTVVGRLSAREPLAADATRFLRAHTERAIKVTLPGPYLLARSI
                     WVKGVSDGAYPSREALADDIVAILRDEIARLVSAGADFVQIDEPVLSNVVFGKPVAVR
                     SFMCAPLAPPSSDVAGELRWAVELINAVVDGSPIRTGVHICRGNWTRDESALLAGDYD
                     ALRRTLDAMDVDQWVLEFATPRAGDLHMFLDGPDREIGLGVVNPRIDTIESPEAIASR
                     VRQAMFSFDASRIWLNPDCGFGTFAERPVADLAIATAKVRAMAAAAGALRREAHAAVP
                     A"
     gene            1812..2165
                     /locus_tag="VFV20_06565"
     CDS             1812..2165
                     /locus_tag="VFV20_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017617456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEU5288048.1"
                     /translation="MDRRVLGLAGEHLAEHELVRRGMRVVARNVRTRIGEIDLVCRDR
                     DGYAFVEVKTRRAGSFVAALEAVDRRKAERLAVLAESWLAQRGERHAPWRILIAALTV
                     SEGGTLVELLPLETS"
     gene            complement(2162..>2653)
                     /locus_tag="VFV20_06570"
     CDS             complement(2162..>2653)
                     /locus_tag="VFV20_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288049.1"
                     /translation="ALPWAVAAVVLANAAIFLVSDTPLSARDVVRKDRGTIERLEYLQ
                     TAPDLDRATLIAAYDALVLVRYSGPEAFPRSFGVHNVVGYDPASAPIEFVFGTRECAP
                     GVGTRDGCAHSPVLAVWDDLIRVHGAGWETATMPHGAKLRLVRNATGARVRIDGLDVT
                     VTR"
BASE COUNT          402 a          935 c          933 g          383 t
ORIGIN      
        1 tacacaggac gcgatgccgc cggcgttctg agcggcgcgg gagggatgca tggccaccgc
       61 caccaagcag ctcaacggcc ttgacctcga ggcgatgggg acgctcgtcc agacggtcac
      121 gaaggatccg tccgcggccg accccctcaa cgactggagc gcccgcgtcc gctgggagtc
      181 gggcttccgc tcgaaggcac tcgtccggaa ccacacgttc accgtcgacg agccggcgac
      241 gctcatcggc gaggatctcg cgccgaacgc ggccgagtac gtgctcgcgg catacggcgc
      301 ctgcctgacc accggcatgg ccctcaacgc gaccaagaac ggcgtgaagc tccgcaacgt
      361 cgaggtcgtg gtcgagggca gcctggacaa catcctgacc ttcttcggga tgtcggaccg
      421 tggccacccc ggattcaggg agatctcggt caaggcgtac atcgacgccg acaccgacga
      481 cgccacgctg aacaggatct ggcgcgagac cgtcgcgagc tcgccggtcg gcaacacgtg
      541 cgtgcggaac gtcgtgctgc gcgacgagtt ggtgcgtgtc tagggagctc ccgctcttcc
      601 cggtcacgac ggtcgggagc tggccacgtc ccgcatctct gatccgggcg ctcaaggaca
      661 agaacgccgg ggcgatcgac caggcggagt tcgaggccgc cgcggacgcg gcggtgctgg
      721 agatcctccg cgtgcaggag gacgccggcg tggacgtcgt gaccgacggc gagcagcggc
      781 gcgacaactt ctactcgttc ctcgcggaca agctcgacgg cgtacggctg atgaccatgg
      841 ccgagctcat cgactacgcc gaggacaaag ccttcttcca ccgcacgctg cggtcgctcg
      901 acgcgccatc gttcgtcatc aagagcccca cggtcgtcgg caggctgagc gcccgcgagc
      961 ccctcgccgc cgacgcgacg cggttcctcc gcgcgcacac cgagcgtgcg atcaaggtga
     1021 cgctgccggg accctatctg ctcgcacgat ccatctgggt caagggcgtg tcggacggcg
     1081 cgtacccgtc gcgcgaggcg ctcgccgacg acatcgtggc gatcctccgc gatgagatcg
     1141 cgcggctcgt cagcgcgggc gcggacttcg tgcagatcga cgagccggtg ctgtcgaacg
     1201 tcgtgttcgg caaaccggtc gcggtgcgca gcttcatgtg cgcaccgctc gcgccgccct
     1261 cgagcgacgt ggcgggcgag ctccgatggg cggtcgagct catcaacgcg gtggtcgacg
     1321 gatcgccgat ccgtacgggg gtgcacatct gtcgcggcaa ctggacgcgc gacgagagcg
     1381 cgctgctggc gggcgactac gacgcgctcc gccgcacgct ggacgccatg gacgtggacc
     1441 aatgggtgct cgagttcgcc acgccgcgcg ctggggacct ccatatgttc ctcgacggac
     1501 ccgaccgtga gatcgggctc ggtgtggtga atccgcgcat cgacaccatc gagtcacccg
     1561 aggcgatcgc gtcacgggtc cgccaggcga tgttctcctt cgacgcctcg cgcatctggc
     1621 tcaacccgga ctgcggcttc ggcaccttcg ccgagcgccc ggtcgcggac ctcgcgatcg
     1681 ccacggcgaa ggtccgcgcg atggccgccg cggcgggcgc gctgcgtcgg gaagcgcacg
     1741 ccgcggtgcc cgcctagacc gctcgcgacc cacacttccg atcgccgcaa gggccggtcg
     1801 ctatcgcgtg cgtggatcgt cgcgtcctcg ggctcgccgg cgaacacctt gccgagcacg
     1861 agctcgtgcg caggggcatg cgcgtcgtcg cgcgcaacgt ccggacccgc atcggcgaga
     1921 tcgatctcgt ctgccgtgac cgcgacggct acgcgttcgt cgaggtgaag actcgccgcg
     1981 cgggatcgtt cgtcgcggcg ctcgaggcgg tggaccggcg caaggccgag cggctcgcgg
     2041 tgctcgcgga gtcctggctc gcgcagcgcg gcgagcgaca tgctccgtgg cggatcctca
     2101 tcgccgcgct caccgtgagc gagggcggga cgttggtcga gctgctgccg ctggagacca
     2161 gctagcgcgt gaccgtcacg tcgagcccgt cgatgcggac gcgcgcgccc gtcgcgttgc
     2221 ggacgagccg cagcttcgcc ccgtgcggca tcgtggccgt ctcccagccg gcaccgtgca
     2281 cgcggatgag gtcgtcccac accgcgagca cgggcgaatg cgcgcagcca tccctcgtgc
     2341 cgacccccgg cgcgcactcc ctcgtgccga acacgaactc gatcggcgcg ctcgccgggt
     2401 cgtacccgac cacgttgtgg acgccgaacg agcgcgggaa tgcctcgggc ccgctgtagc
     2461 gaacgaggac gagcgcgtcg tacgccgcga tgagcgtcgc ccgatcgagg tcgggtgcgg
     2521 tctgcaggta ctcgagccgt tcgatcgtgc cgcggtcctt gcggacgacg tcacgcgcgg
     2581 agagcggcgt gtcgctcacg aggaagatcg ctgcgttcgc gaggacgacc gccgcgacgg
     2641 cccatgggag cgc
//
LOCUS       DASTZR010000292         5866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_173033, whole genome shotgun sequence.
ACCESSION   DASTZR010000292 DASTZR010000000
VERSION     DASTZR010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5866
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173033"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..3648)
                     /locus_tag="VFV20_06575"
     CDS             complement(67..3648)
                     /locus_tag="VFV20_06575"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEU5288050.1"
                     /translation="MDDTARADRRARLAILGVIALGVALRLLVLRAQGFPSDVGTFQA
                     WAERLVEVGPARFYEPGYFSDYPPGYLYVLWLLGALFDGEFLRLAVKAMSIPADVAIA
                     IIAAALAWRAAGRSSAILAAGLWMLSPGPIFAGPYWGQVDAVGTVPLMGSLLLAARRR
                     WALAGLLAGVALMVKPQFGIALLVVAVAALVEWLRKADWRPPVRAGLASAGTILALGL
                     PFRAGPAELIELVRSASATYPYTSLYAFNIWSVVADFWKPDDDYVVIGGVLLVTGLLA
                     ACAPLWRRRDTATLLAAGALASFAFYFLPTRAHERYLFPAFALLLPLAAVRARLLLPY
                     VLLAISFAASLFFAFTRYPQNDLRSPALLESTLFTRNGQIALALLMLALAAYLAWRLW
                     RHDATLEAEREPAPPPIGAVVAHPDREPWRPPALLALGRVPTRRDITVALLVALVVLV
                     TRGYRLDQPREMYFDEVYHARTAFELLAQRDPYEWTHPHLAKEVMALGILAFADDRVV
                     GTEPPMPLAVRFAVANDGTRAFAEPGQLIIRGRDGRERARVKIAAEDGEPKALAFTGG
                     DVVLATDRRITRYAADGRTVASVPRPDVGAMSSLVADGTRVYIGGDKGIALYQTLDAP
                     PQLIARPVVALTAKPDGSELYALEANGVVRRIDPVTGQDRETLNGRGPGSAIAYAQGA
                     NRLFIARSDTPALDVFDFDEHSTDTVPLASDRTGQLAGPASSLALVPRTQFLYALAPG
                     KVVVIEPQGASPYAAIAVNASDRFLAVDGEDDKLLAAGSDSVERIETGRHALAWRLPG
                     VLFAAILAFFLVLLARRLFASPAVAYLVGALVVLDGSMYAQARIGMNDIYVTTFIVAA
                     WYFIVAAHRPRRRAWMDLVIAGVLVGLGAASKWAAVYTLAGIFVACVAVTIWAYERGR
                     PGTGGPLDLFAGRGRGAALLFGSFAVIPAVIYLGAYQRWFGGATAPYGWNLWELTQQM
                     YWYHSSLTSPHCAGAPWWSWPLDLKPTYWYFGASVAGWNGYIYDAGNPILFWGALPAA
                     AIVGGYAVRARSATLGLVTLAMLAQFVAWIPISRVLFFYHFFTVLPFYLLCLAIVLAL
                     IGARRPLAVFAYLVVAAFAFLAFYPYVSGVPIPGELGSAYQILPTWQYDPAFWPTESC
                     PTSVLQSPATALTVGVAWLYEMACLGLGIGVAVGARPFRKRLAALGL"
     gene            complement(3651..4832)
                     /locus_tag="VFV20_06580"
     CDS             complement(3651..4832)
                     /locus_tag="VFV20_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288051.1"
                     /translation="MSDRAVAVTLGAIACVVYLAAGFGLLTDYDYYGRLGQALLSGRW
                     WLTEAPPWLNELVECGDGRFCVAYPPLPAILAAPLAALLPTALAQVLVSRLAGGASAG
                     VLYLALRALGVPRATAIWGTVLSAVGTTMFFSTVDGRAWFDAHAVAMPFLAAAVLFAA
                     RGNAGWLVGAAIGLAALSRLPVAAAAPALAMLLARRAGTPFPRALGGVVLGGLPFAAV
                     YVAYNVLRWGTPFDEGYTRLAEGDFFFTHGVFSPFYIPRQLYAIFIEPPEFVDGSPWF
                     LRPSFRGMSLFLTTPAFLWLFAGLRHVRRDPVIALTAGAALLALLPDLLHATVGFAQF
                     GYRFSLDAQPFLITLALAGDARGADGWRSRPSWLFVVAILLSIAINLYGTIAITRFGY
                     W"
     gene            complement(4870..5577)
                     /locus_tag="VFV20_06585"
                     /pseudo
     CDS             complement(4870..5577)
                     /locus_tag="VFV20_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013139441.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
     gene            complement(5650..>5866)
                     /locus_tag="VFV20_06590"
     CDS             complement(5650..>5866)
                     /locus_tag="VFV20_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional nuclease domain-containing protein"
                     /protein_id="HEU5288052.1"
                     /translation="FLQLGEKEVEIDCRPSDAIALAVRCDARILVADTVLDRSAILAE
                     SEEDQNLTVFKEFIESLPDDPQAPPTS"
BASE COUNT         1016 a         2016 c         2169 g          665 t
ORIGIN      
        1 ccctggtgct cgcgctggcc gccgcgctgg tcgcgctggc ctcactcgcc ggcctcgcgg
       61 gctgacctac agtccgagcg ccgcgagacg cttgcggaac ggccgcgccc ccaccgccac
      121 cccgatgccc agccccaggc aggccatctc gtacagccat gcgacgccga cggtgagggc
      181 cgtcgccggg ctctgcagca ccgatgtcgg gcatgactcg gtcggccaga atgccgggtc
      241 gtactgccag gtcggcagga tctggtacgc ggatccgagc tcgccaggga tcgggacgcc
      301 ggagacgtac gggtagaacg ccaggaacgc gaacgcggcg accacgaggt acgcgaagac
      361 ggcgaggggc cggcgggcgc cgatgagcgc cagcacgatc gcgaggcaga gcaggtagaa
      421 cggcagcacc gtgaagaagt ggtagaagaa cagcacgcgt gagatcggga tccacgcgac
      481 gaactgcgcg agcatcgcga gcgtgacgag gcccagcgtc gccgagcgcg cccgcaccgc
      541 gtagccgccg acgatcgcgg cggcggggag ggcaccccag aagaggatgg ggttgccggc
      601 gtcgtagatg tacccgttcc aaccggccac gctcgcgccg aagtaccaat acgtcggctt
      661 gaggtcgagc ggccagctcc accagggcgc acccgcgcag tgcggcgacg tcagcgacga
      721 gtgataccag tacatctgct gcgtgagttc ccagaggttc cagccatagg gggcggtggc
      781 accaccgaac cagcgctgat acgcgccgag gtagatcacc gcggggatca ccgcgaacga
      841 tccgaagagc agcgccgcgc cgcgcccacg gcccgcgaac aggtcgagcg gcccgccggt
      901 acccgggcgg ccacgctcgt acgcccaaat cgtcacggcc acgcacgcga cgaagatccc
      961 ggcgagcgtg tacacggcgg cccacttcga cgccgcgccg aggccgacga gcactccggc
     1021 gatgacgagg tccatccacg cccggcggcg ggggcgatgc gcggcgacga tgaagtacca
     1081 ggccgcgacg atgaaggtcg tcacgtagat gtcgttcatg ccgatccgcg cctgcgcgta
     1141 catcgatccg tcgaggacga cgagcgcgcc gacgagatac gcgacggccg gcgacgcgaa
     1201 gagacgccgc gcgagcagca cgaggaagaa cgcgaggatc gcggcgaaca ggacgccggg
     1261 caggcgccag gcgagcgcgt gacggccggt ctcgatccgc tcgacggagt cgcttcccgc
     1321 ggcgagcagc ttgtcgtcct cgccatcgac ggcgaggaag cggtcggagg cgttcaccgc
     1381 gatcgccgcg tagggcgacg cgccctgggg ctcgatgacg acgaccttgc cgggagcgag
     1441 cgcgtagagg aactgcgtcc ggggcacgag cgcgagcgac gacgccggtc cggcgagctg
     1501 cccggtccgg tcgctcgcga gcggcaccgt gtcggtcgaa tgctcgtcga agtcgaagac
     1561 gtcgagcgcg ggtgtatcgg agcgcgcgat gaagagacgg ttcgcaccct gcgcgtacgc
     1621 gatcgcggac ccgggaccgc gcccgttcag cgtctcgcga tcctgtcccg tcacggggtc
     1681 gatgcgccga acgaccccgt tcgcctcgag cgcgtacagc tcgctgccgt ccggcttcgc
     1741 cgtgagggcg acgacgggcc gcgcgatcag ctgcggcggc gcgtcaagcg tctggtagag
     1801 cgcgatgccc ttgtcgccgc cgatgtagac gcgcgtcccg tccgccacga gcgagctcat
     1861 cgcgcccacg tccggccgcg ggacgctcgc gacggtccgc ccgtcggcgg cgtaccgcgt
     1921 gatgcgacgg tcggtcgcga ggacgacgtc cccgccggtg aaggccaggg ccttcggctc
     1981 gccatcctcg gccgcgatct tcacccgagc gcgctcgcgt ccgtcgcggc cccggatgat
     2041 gagctggccg ggctcggcaa aggcgcgcgt cccgtcgttc gcgaccgcga agcggacggc
     2101 cagcggcatg ggcggttccg ttccgacgac gcgatcgtcc gcgaacgcga ggatgccgag
     2161 cgccatcacc tccttcgcga gatgtggatg ggtccactcg tacggatcgc gctgcgcgag
     2221 aagctcgaac gcggtccgcg cgtggtagac ctcgtcgaag tacatctcgc gcggctggtc
     2281 gagacggtat ccacgggtga cgagcacgac gagggcgacg aggagcgcca ccgtgatgtc
     2341 gcggcgcgtc gggacacgtc cgagcgcgag cagcgcggga ggccgccacg gctcgcgatc
     2401 cggatgcgcg accaccgcgc cgatcggcgg cggcgcgggc tcacgctcgg cctcgagcgt
     2461 cgcgtcgtga cgccagagac gccacgcgag gtaggccgcg agcgcgagca tcagcagcgc
     2521 cagcgcgatc tgcccgttgc gcgtgaacag cgtgctctcg agaagggccg gcgagcgcag
     2581 atcgttctgc gggtaccgcg tgaacgcgaa gaagaggctc gccgcgaagg agatcgcgag
     2641 aaggacgtac ggcaggagga gccgggcgcg caccgccgcg agcggcagga gcagcgcgaa
     2701 ggccgggaac aggtagcgct cgtgcgcgcg cgtcggcagg aagtagaacg cgaaggacgc
     2761 gagcgccccg gcggcgagca gcgttgccgt gtcgcgccgg cgccacagcg gggcacacgc
     2821 ggcgaggagt cccgtgacca gcaggacgcc gccgatgacc acgtagtcgt cgtccggctt
     2881 ccagaagtcg gccacgacgg accagatgtt gaacgcgtac aggctcgtgt acgggtacgt
     2941 cgccgacgcc gaccgcacga gctcgatgag ctccgccgga cccgcgcgga acgggagacc
     3001 gagcgccagg atcgtgccgg ccgacgcgag accggcgcgc accggcggac gccagtcggc
     3061 cttccggagc cactccacca gcgccgccac ggccacgacg agcagggcga tgccgaactg
     3121 cggcttcacc atgagcgcga cgcccgccag cagtcccgcg agcgcccagc gtctccgtgc
     3181 ggccagcagc agcgacccca tgagcggcac ggtgccgacc gcgtcgacct ggccccagta
     3241 cggcccggcg aagatcgggc ccggcgacag catccagagc cccgccgcga ggatcgcgct
     3301 cgaccggccc gctgcgcgcc acgcgagcgc cgccgcaatg atcgcgatcg cgacgtcggc
     3361 cgggatggac atggccttca ccgcgagacg gaggaactcg ccgtcgaaga gcgcgccgag
     3421 cagccacagc acgtagaggt acccgggcgg gtagtcgctg aagtaccccg gctcgtagaa
     3481 gcgcgccggg ccgacctcca ccaggcgctc cgcccacgcc tggaacgtcc cgacatcgct
     3541 cgggaagccc tgcgcgcgca ggacgagcag ccgcagcgcg acgccgagcg cgatgacgcc
     3601 caggatcgcg agacgcgcgc gacgatcggc gcgcgcggtg tcgtccatca ctaccagtat
     3661 ccgaatcgcg tgatcgcgat ggtgccgtag aggttgatcg cgatcgacag caggatcgcc
     3721 acgacgaaga gccacgacgg tcgactccgc catccatccg cgccgcgcgc gtcgccggcg
     3781 agggcgaggg tgatgaggaa gggctgcgcg tcgaggctga agcggtagcc gaactgcgcg
     3841 aacccgacgg tggcgtgcag gaggtccggg aggagcgcga gcagcgccgc gccggcggtg
     3901 agcgcgatca cgggatcgcg gcggacgtga cgcagtccgg cgaagagcca caggaacgcg
     3961 ggcgtcgtga ggaagagcga catgcccctg aagctcgggc gcaggaacca cggcgagccg
     4021 tccacgaact ccggcggctc gatgaagatc gcgtagagct ggcgcgggat gtagaagggc
     4081 gagaacacgc cgtgcgtgaa gaagaagtcg ccctccgcaa ggcgcgtgta gccctcgtcg
     4141 aagggcgtgc cccagcgcag gacgttgtag gcgacgtaga ccgcggcgaa gggcaggccg
     4201 ccgaggacga cgccgccgag cgcacgtggg aagggcgtgc cggcgcgacg cgccagcagc
     4261 atggcgagcg cgggcgccgc cgcggccacg ggcaggcggg acagcgcggc gagaccgatc
     4321 gcggcgccca cgagccagcc ggcgttcccc cgcgccgcga agagcaccgc ggcggcgagg
     4381 aatggcatcg cgaccgcgtg cgcgtcgaac cacgcgcggc catcgacggt cgagaagaac
     4441 atcgtggtgc cgaccgccga gagcaccgtg ccccagatcg cggtcgcacg gggcaccccg
     4501 agcgcgcgca gcgcgagata gaggacgccc gcggacgcgc cgcctgccag ccgcgacacc
     4561 agcacctggg caagcgccgt cggtagcaac gcggcgagcg gggcagcgag gatcgccggc
     4621 agcggggggt aggcgacgca gaagcgtccg tcgccgcact ccacgagctc gttgagccac
     4681 ggcggcgcct cggtgagcca ccaccggccc gagaggagcg cctgccccag gcggccgtag
     4741 tagtcgtagt cggtcagcag gccgaagccg gccgcgagat agaccacgca cgcgatcgcc
     4801 ccgagcgtga cggcgacggc gcggtcggac accggttcag ggcttccgcg cgcgcccgct
     4861 gccgaatcga agacgcatga cgccgcggag gtcgtccatc gcggtgcgga tgatgtcgac
     4921 gcgcgaatcg ggatcgtccg tccagtccac cggtacctcg tgcacgcgca gcccggcgcg
     4981 ctgcgcgagg atgagcagct cggtgtcgaa gaaccaggtc tgatcctcga tgcggggaac
     5041 gagatccttt gccaccgacg cccgcgcggc cttgaagccg cattgcgcgt cgctgaagcc
     5101 cgcgcccagc gcaagacgca ggatcagcat gtacgcgcga gagatgagct cgcgcttcgg
     5161 accgcggcgc gtgcgtgcgc cgcgcgcgag gcggctgccg atggcgatgt cgctgtgtcc
     5221 ggacacgagc ggcgcgacca gcggcaggag cgccttcaga tcggtcgata ggtcgacgtc
     5281 catgtacgcc acgacgagcg cgtcgctctt cagccacgct gcccggagcg cacggccacg
     5341 gcccttcgcg tccagatgca ggacccggac gcccggaaga tcggcggcca gacgctctgc
     5401 gaccgcgagg gtgccgtccc ggctcgcgtt gtcggcgatc gtgatgaccg atgggaacgg
     5461 catcgaccgt gcgaggaacc cgtggaggcg acgaacgctc ggctcgaggt cgcgcacctc
     5521 gttgtagaca gggatgacga cgtcgagcag cggctcgccg gcctccgatc tggccatggg
     5581 tatcacggac acggcaccat gctgcgtcat cgcgccgctg tcatgcacag gaacgagaca
     5641 ggtccgcttc tagctggtcg gcggtgcctg cggatcatct gggagcgact cgatgaactc
     5701 cttgaacacc gtcaggttct ggtcctcttc gctctcggcg aggatcgcgg agcggtcgag
     5761 caccgtgtcg gccacgagga tccgcgcgtc gcagcggacc gcgagggcga tggcgtcgga
     5821 ggggcgacag tcgatctcga cctccttctc accgagttgc aggaag
//
LOCUS       DASTZR010000293         4143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_325909, whole genome shotgun sequence.
ACCESSION   DASTZR010000293 DASTZR010000000
VERSION     DASTZR010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325909"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /gene="eno"
                     /locus_tag="VFV20_06595"
     CDS             complement(<1..206)
                     /gene="eno"
                     /locus_tag="VFV20_06595"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997616.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU5288053.1"
                     /translation="MPDTTIELIRAREILDSRGDPTVAVDVLLSDGVRGTAMVPSGAS
                     TGAHEAVELRDGDRRRYRGKGVRRA"
     gene            complement(199..1422)
                     /locus_tag="VFV20_06600"
     CDS             complement(199..1422)
                     /locus_tag="VFV20_06600"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU5288054.1"
                     /translation="MGGTTSGPSARTIRDVDPSGKKVLVRVDFNVPLEADGRGGMAVA
                     DDTRIRAALPTITSLLERGAAIALCSHLGRPKGPDPKQSLAPVAVRLAELLGKPVRLL
                     RDSAGADVREAVAKLRGGDIVVLENVRFHPEEEKNDPAFAKELAAGFDLYVNDAFGAA
                     HRAHASTAGVAAILPAYAGLLMEKELTALGGLLADPKRPFVAIIGGAKVSSKIDVLRS
                     LVPKIDTLAVGGGMANTFLLATGKKVGTSLVEADRVDEASRIIEEIEDAKKRILLPVD
                     VLCAPSVDADGAQRAATYDVDKVPEGTAIVDIGPRTVAAYRDAIREAKTVFWNGPVGV
                     FEIDAFANGTRAVARFMANNPTATTVVGGGESVQAVTEAGLADKMTHVSTGGGASLEL
                     IEGKPLPGVEAIPDA"
     gene            complement(1419..2450)
                     /gene="gap"
                     /locus_tag="VFV20_06605"
     CDS             complement(1419..2450)
                     /gene="gap"
                     /locus_tag="VFV20_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU5288055.1"
                     /translation="MGARVAINGFGRIGRQFLKAVLERHPDLEVVAINDLADPKMNAL
                     LFKRDSNYGTFKGEVSATADAIVVDGRSIKVIQERDPAKLPWKDLNIDIVIESTGFFT
                     DATKAKAHRDAGAKKVIISAPAKNEDKTIVLGVNEKEYDAEQHNIISNASCTTNGLAP
                     VAKVLNDTFGIEKGFLTTVHSYTNSQKVLDLVASDPRDARAAAMNIVPAATGAAKAVA
                     LVIPELQGKFTGMAFRVPTPTVSVVDFVALLKRDVTKDEVNGAFKKASEGELKGIMDY
                     TEEPLVSMDLKGDEHSTIVSGMDTIVLGNMVKVISWYDNEWGYACRLADITAFVASKL
                     RVPAGAGSR"
     gene            complement(2487..3455)
                     /locus_tag="VFV20_06610"
     CDS             complement(2487..3455)
                     /locus_tag="VFV20_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU5288056.1"
                     /translation="MSAQPFLGIDLGGTHIRSVVAMAGDGIPNIVSRDTRPTPREGAD
                     AIVGAIVASAADVMGRANVSAREVVAAGCSSPGPLDHVSGVVIETPNLVDFRNVALAD
                     RLSSALGITVYVDRDTCMAAIAEGLVGAARGAKDYVYVTVSTGLGGAIVSGGRMMRGA
                     TNTAGEIGHFPVAFQLDTSRGAGDDVPRCGCGSYGCAEAFAAGAKIAEAYGASDAGEV
                     FAAAERGDARARTIVVRAERALANLAVGLVNVMNPTIIVVGGSVAEHQTAHVLDPMRE
                     AIRTRAFAAPAVAARLVPAALGGDVGMLGAVLEARERVAGRGDWFL"
     gene            complement(3452..>4143)
                     /locus_tag="VFV20_06615"
     CDS             complement(3452..>4143)
                     /locus_tag="VFV20_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288057.1"
                     /translation="SPPAADTWTAPATEWTPPQREQWTPAPEPTAPPADDPRLGALTM
                     PPAPVAEPEAERPAPSAANEWAPPAPAEAPPQRKGLFGRFRKDDGVAPTAAPMMERVA
                     GTRAGKLAALSNTLLGEFNSGTYGKARIDDRIGSLLMRVDEQADPIDRPLPIVDDRLD
                     VQALERVNLPEPQIVPYLALLVSTIYADAEKAFGRDKAKKGYRAAQQQVFAGDTSALA
                     GPDLAGKLPKV"
BASE COUNT          584 a         1458 c         1437 g          664 t
ORIGIN      
        1 gcgcgacgga cgcccttacc gcggtagcgc cgacggtcgc cgtcccggag ctcgaccgcc
       61 tcgtgcgcac ccgtggaggc gcccgacggg accatcgcgg tgccccgcac gccgtcgctc
      121 agcagcacgt ccaccgcgac cgtgggatcg ccgcggctgt cgaggatctc acgcgcgcgg
      181 atcagctcga tcgtggtatc aggcatccgg gatggcctcc acaccgggca gcggcttccc
      241 ttcgatgagc tcgagcgacg cgccgccgcc ggtggagaca tgcgtcatct tgtcggccag
      301 gccggcctcc gtgacggcct gcacggactc gccgccgccg accaccgtgg tcgcggtggg
      361 attgttcgcc atgaagcgcg cgaccgcgcg ggtcccgttg gcgaacgcgt cgatctcgaa
      421 gacgcccacc gggccgttcc agaacaccgt cttcgcctcg cggatcgcgt cgcgataggc
      481 cgcgaccgtg cgcgggccga tgtcgacgat cgcggtgccc tccggcacct tgtcgacgtc
      541 gtacgtggcc gcgcgctggg caccatcggc gtcgacgctc ggcgcgcaga gcacgtcgac
      601 cgggagcagg atgcgcttct tcgcgtcctc gatctcctcg atgatccgcg acgcctcgtc
      661 gacgcgatcc gcttcgacga gcgaggtccc gaccttcttg cccgtcgcga ggaggaacgt
      721 gttggccatc ccgccgccga ccgcgagggt gtcgatcttg gggacgaggg accggagcac
      781 gtcgatcttc gacgacacct tcgcgccgcc gatgatcgcg acgaacgggc gcttcggatc
      841 ggcgaggagt cccccgagcg ccgtgagctc cttctccatc agcagcccgg cgtacgcggg
      901 aaggatggcc gcgaccccgg cggtggaggc gtgcgcgcgg tgggccgcgc cgaaggcgtc
      961 gttgacgtag agatcgaacc cggcggcgag ctccttcgcg aacgcgggat cgttcttctc
     1021 ctcctcggga tggaagcgca cgttctccag gacgacgatg tcgccaccgc ggagcttcgc
     1081 gaccgcctca cgcacgtccg cgccggcgga gtcgcgcagc agccggaccg gcttcccgag
     1141 cagctcggcg agccgcaccg ccaccggagc gaggctctgc ttcggatcgg gacccttcgg
     1201 acggccgagg tgggagcaca gcgcgatcgc ggcaccgcgc tcgagcagcg aagtgatggt
     1261 cggcagcgcg gcacggatgc gggtgtcgtc cgcgacggcc atgccgcctc tgccgtccgc
     1321 ctccagcggg acgttgaagt cgacgcgaac gaggaccttc ttcccggacg ggtcgacgtc
     1381 ccggatggtc ctcgccgatg gaccgctcgt cgttccgcct atcgagagcc ggcccccgcc
     1441 ggaacgcgaa gcttggacgc gacgaacgcg gtgatgtcgg cgagacggca cgcgtagccc
     1501 cactcgttgt cgtaccagga gatgaccttg accatgttgc ccaggacgat cgtgtccatg
     1561 ccgctcacga tggtggagtg ctcgtcgccc ttcaggtcca tcgagacgag cggctcctcg
     1621 gtgtaatcca tgatgccctt cagctcgccc tcggacgcct tcttgaacgc gccgttcacc
     1681 tcgtccttcg tcacgtcacg cttcagcaac gcgacgaagt cgacgacgga gaccgtcggc
     1741 gtggggacgc ggaacgccat tccggtgaac ttcccctgca gctccgggat gacgagggcg
     1801 acggccttcg ccgcgcccgt cgcagcgggg acgatgttca tggccgccgc gcgcgcgtcg
     1861 cgcggatcgc tcgcgacgag gtcgaggacc ttctgcgagt tcgtgtacga gtggacggtg
     1921 gtgaggaagc ccttctcgat gccgaacgtg tcgttgagga ccttcgcgac cggcgcgagg
     1981 ccgttcgtgg tgcacgacgc gttgctgatg atgttgtgct gctccgcgtc gtattccttt
     2041 tcgttcacgc cgaggacgat ggtcttgtcc tcgttcttcg ccggcgccga gatgatgacc
     2101 ttcttcgcgc ccgcgtcccg gtgcgccttc gccttcgtcg cgtcggtgaa gaagcccgtc
     2161 gactcgatga cgatgtcgat gttcaggtcc ttccacggga gcttcgccgg atcgcgctcc
     2221 tggatgacct tgatgctgcg gccgtccacg acgatggcgt cggccgtggc cgagacctcg
     2281 cccttgaacg tgccgtagtt ggagtcccgc ttgaacagca gtgcgttcat cttcggatcg
     2341 gcgagatcgt tgatggcaac gacctcgaga tcggggtgcc gctcgaggac cgccttgagg
     2401 aactggcgac cgatgcgacc gaagccgttg atcgcgaccc gcgctcccat gtgaaccccc
     2461 cttgtgtgtg gcacttgaaa ctctaactaa aggaaccaat cgccgcgtcc cgcgacgcgt
     2521 tctcgtgcct ccagcacggc accgagcatg ccgacgtcgc cgcccagggc ggcgggcacg
     2581 agccgcgcgg cgaccgcggg cgccgcgaag gcgcgcgtgc ggatcgcctc gcgcatcgga
     2641 tccaggacgt gcgccgtctg gtgttcggcg accgatccgc cgacgacgat gatcgtcggg
     2701 ttcatgacat tgacgagacc gacggcgaga ttcgcgagcg cgcgctccgc acgaacgacg
     2761 atcgtgcgcg cccgcgcgtc accacgttcg gccgcggcga agacctcgcc cgcatccgac
     2821 gcgccatatg cctccgcgat cttcgccccg gcggcgaacg cttctgcgca gccgtaggag
     2881 ccacagccgc accgcggtac atcgtcaccg gcgccacgtg aggtgtcgag ttggaacgcg
     2941 actgggaaat ggccgatctc cccggccgtg ttcgtcgcgc cgcgcatcat gcggccgccg
     3001 ctcacgatgg cgccgcccag gccggtcgag acggtcacgt acacgtagtc cttggcgccg
     3061 cgcgccgcac cgaccagacc ctcggcgatc gcggccatgc aggtgtcgcg gtccacgtac
     3121 accgtgatgc cgagcgccga cgagagccgg tccgcgagcg cgacgttccg gaagtccacg
     3181 aggttcggtg tctcgatcac gacgccgctc acgtggtcga gcggccccgg cgacgaacat
     3241 cccgccgcga ccacctcgcg cgcggagaca ttcgcgcgcc ccatcacgtc ggcggcactc
     3301 gcgacgatcg cgccgacgat cgcgtcggca ccctctcgtg gcgtcggcct cgtgtcgcgc
     3361 gacacgatgt tcgggatgcc gtcaccggcc atcgcgacga cgctgcggat atgagtgccg
     3421 ccgaggtcga tgccgaggaa gggctgcgcg ctcagacctt cggcagcttc cccgcgagat
     3481 ccggcccggc gagcgccgac gtgtcaccgg cgaatacctg ctgctgtgcg gcccgatagc
     3541 ccttcttcgc cttgtcgcgc ccgaaagcct tctccgcgtc cgcgtagatg gtggagacga
     3601 ggagcgcgag atagggaacg atctgcggct cggggaggtt gacgcgctcg agcgcctgca
     3661 cgtcgagtcg atcgtcgacg atgggcagcg gccggtcgat cgggtcggcc tgctcgtcga
     3721 cgcgcatgag caggctgccg atgcggtcgt cgatcctcgc cttcccgtag gtgccgctgt
     3781 tgaactcacc gaggagcgtg ttcgagagcg cggcgagctt gcccgcgcgc gtgcccgcga
     3841 cgcgctccat catcggcgcg gcggtgggcg cgacgccgtc gtcctttcgg aaacggccga
     3901 agaggccctt ccgctgcggc ggcgcctccg cgggcgcggg cggcgcccac tcgttcgcag
     3961 ccgacggcgc ggggcgctcg gcctccggtt cggcgacggg agcgggcggc atggtgagcg
     4021 cgccgagacg tgggtcgtcc gccggcggcg ccgtgggctc cggcgccggc gtccactgct
     4081 cgcgctgggg cggcgtccac tcggtcgcgg gtgcggtcca cgtgtccgcg gcgggtggcg
     4141 acg
//
LOCUS       DASTZR010000294         7141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_147276, whole genome shotgun sequence.
ACCESSION   DASTZR010000294 DASTZR010000000
VERSION     DASTZR010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7141
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147276"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1778)
                     /locus_tag="VFV20_06620"
     CDS             complement(<1..1778)
                     /locus_tag="VFV20_06620"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEU5288058.1"
                     /translation="MLERWGRLVYRRRWILLLVSAVLLAASAVVALQGGDLQDPESLN
                     ASESGRASSLLNAQLPRATSAAVPVGTTFLLVFESDDRSVDEPRYREAVLDAVAPLRS
                     DPRVQSMRTYYDAPAQSASLVSRDGRRTVVSVTLRDFRAVADHYYDDLRGEIHSDTLR
                     IYATSNLPINHDLNRILDADLRRAEVVALPIALVLLLLVFGTLVGALLPLGVGLLAIV
                     AGIAGVFLLSRVMDVSPYSLNIVTLIGLGTAIDYSLFVVARFREELRHGDVEDALART
                     MGTAGRAIIFSGLTVAIGLSGMLFFAGTFVSSLGVAGSIVVAFAVLYAVTFLPALLAI
                     LGRRVNALRIPVRRSASVGRGFWHAVATTVMRRPLLVLLPTVAVVLLAGSPFLEFRLA
                     TADTRALPPQAESRRGYDLLVANFPGQEQSTVTVVVHWPDGDPRSADATAYVTRVSDV
                     YRRLPNVIRVNPPTFGGHIAVLGVVSSVPASTDEARELVRTIRGRSTTVPTMPGPPAG
                     GELLVTGATAFDLDTIDFVAERAVWAVAFIVVLTYLALFGLLGSVVLPLKAVLMNLLS
                     IAASFGALVWIFEQGHFAEQLNFTPQS"
     gene            1873..2910
                     /locus_tag="VFV20_06625"
     CDS             1873..2910
                     /locus_tag="VFV20_06625"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5288059.1"
                     /translation="MSASDVLSALAERLPRGYRARPFEDADREPLVAAGNSEAHPMEQ
                     ETAAEWRRWESMFADDTRVRVVVVSDAGTVVGSGNVQAGWTPRPDGAQHLNVGVHPEH
                     RRRGIGGALLDALEAEGHRRGVPRLLSGVSSARPFAVAFATKHGYREIGRRIMSYVEL
                     DAFEPDRWRESVDRVEREGIRLRPIAEVLAGLDEAGAERFWRAVWEAEAPMWEDIPFS
                     SPTPHWAFERFYKLAAKSGQLLADVSVVAYAGERIVGFTETGDRQGKDGWTWMTGVAR
                     DHRGKGIAMGLKVAALTLAKKKRLRAMCTVNDEPNKAMRGVNIKLGYQPVPDHIELEK
                     RLSAGATAPLR"
     gene            complement(2828..4021)
                     /locus_tag="VFV20_06630"
     CDS             complement(2828..4021)
                     /locus_tag="VFV20_06630"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5288060.1"
                     /translation="MLGRIRTLGPALPVLAAISFVSSLGISIMLPLLPLYGLSLGATP
                     LQLGLLTSAFAVANSVGQFGGGLFVDRFGSRRFIIGGTALYAAANALIATATSAVALI
                     AYRGLAGLGGGSNIIATRLYITQTADPKRLAFFNSILSAATSAGNVFGPAVGGLIAVA
                     ADLRVPFMIVAATSTIALLGATLLPRPERPATAPRPAGEPTWTRAVLVLFAANMLLLV
                     GFGGWITSYAPFATANLGWSTFDVGIIFTIFGLGDIALGPWLGHLADRTGRRRMAIAA
                     SVPVCFFGLALVFGLPRPVVYAISLITGASLTAFNASWFALLTTAVAPERRGRVFGTV
                     NAIANGGVVIGAMGASLLWQAFDVGYGLLLSSFAVLLSGAVLLLLPRDVSPARYGPAQ
                     ADSPA"
     gene            4083..4982
                     /locus_tag="VFV20_06635"
     CDS             4083..4982
                     /locus_tag="VFV20_06635"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5288061.1"
                     /translation="MPDAAIAFEGASKRYRSGTLALHEATWSIPVGSRSCLLGPNGSG
                     KTTSIRLLEGALTPTSGYVSLLGTTVNGDGYALARGRCGIVPQQAGMYSDLTTWEYLE
                     LARRLYGRGSVGDALERFGLTEHGDKRLAQLSGGFQRRVVVAAALLSEPDVLLLDEPT
                     VGLDPVAAREIHDTLRAVMAGRTTLLCTHNLAEAEALCDDVIILRGGEVLLHEPLAEL
                     RQRQHARTRFAARQPAETLAARVRAHGAIATIEDGAVIVPIDARRDAPALLRALLHDG
                     MDVYECAPLEATLEELFLEAVRR"
     gene            4985..5725
                     /locus_tag="VFV20_06640"
     CDS             4985..5725
                     /locus_tag="VFV20_06640"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5288062.1"
                     /translation="MSAIRSLLWKETRQLGRNRTAMLTAALLPFLILFLAPIQMIVGV
                     KLGGDRGMDQLREAPLPGFSDVSDPGQLLVQFFYPMLLVIGGLLLPSLTTTYAIVTER
                     ERRSLELLVSLPVTVGEIVAAKLLSVLVVTAMIGIPYVAVVITLLIVLGLAGPATVPA
                     LLAPFVAAVACSTAIALLLTLLARDFRTANNLNGAFFVPALLLSIGTLAGVGGPARTY
                     VLAGVLLALAAIALFAALRWVSFERYLD"
     gene            complement(5832..>7141)
                     /locus_tag="VFV20_06645"
     CDS             complement(5832..>7141)
                     /locus_tag="VFV20_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5288063.1"
                     /translation="FGAVFFGALGDIIGRKYTFLVTITAMGLSTVIIGFLPTYKDIGV
                     TAAFILVALRLIQGLALGGEYGGAAIYVAEHAPDNKRGLYTSWIQTTATVGFFLALLI
                     ILFFRTNMSAADFTAQGWRYPFWISAVLVAIALYIRIRLRETPLFARLKEANEHVRST
                     GHWARESFSGRKVGTIALVLLGLTAGQGVVWYQGQFQANFFMTTYLKLSYTQSYPIML
                     WAVALGTPFFLVFGWLSDKVGRKPIILLGCLIAAVTYLPIYHAMTDAANVTRDATGLI
                     TKADPNVPLLVFLVWIQVIYVTMVYGPIAAFLVEYFRAKVRYTSLSIPYHFGNGWFGG
                     LLPLLYTGLVGATFPEGSKSYVPFLTNFMGLPIKTDISLRQFNTTGDPNGNIYLGLAY
                     VMIVALMTVVVGTLFLREPRGVKIWHEVGGEQPAAVTEATPAE"
BASE COUNT         1179 a         2428 c         2565 g          969 t
ORIGIN      
        1 gactgcggcg tgaagttgag ctgctccgcg aagtgcccct gttcgaagat ccagacgagc
       61 gcaccgaacg atgcggcgat cgacagcagg ttcatgagca ccgctttgag cggcagcacg
      121 accgaaccga ggagcccgaa gagcgcgagg tacgtgagga cgacgatgaa cgcgacggcc
      181 cagaccgcgc gctccgccac gaaatcgatg gtgtcgaggt cgaaggcggt ggcgcccgtg
      241 acgaggagct cgccgccggc gggcgggccg ggcatcgtcg gcaccgtcgt cgaccgacca
      301 cggatcgtgc gcacgagctc gcgggcctcg tcggtggacg cggggacgct gctcacgact
      361 ccgagcaccg cgatgtgacc gccgaacgtc ggcggattca cgcgaatgac gttcggaagg
      421 cgacggtaga cgtcgctgac gcgcgtcacg taggcggtcg cgtcggcgga gcgtggatcc
      481 ccgtcgggcc aatggactac caccgtgacc gtcgactgct cctgtcccgg gaagttcgcg
      541 acgagcaggt cgtagccgcg gcgcgactcc gcctgcggcg gaagcgcacg cgtatcggcg
      601 gtcgcgaggc ggaactccag gaacggcgag cccgcgagca gcacgacggc gaccgtcggc
      661 agcaggacga ggagcgggcg ccgcatcacc gtcgtggcca ccgcgtgcca gaatccgcga
      721 ccgaccgacg ccgatcgccg caccgggatg cggagcgcgt tcacgcgccg cccgaggatg
      781 gcgaggagcg ccggcaggaa cgtcacggcg tagagcacgg cgaacgcgac gacgatggac
      841 ccggcgaccc cgagcgagga cacgaaggtc cccgcgaaga acagcatccc ggagaggccg
      901 atcgcgacgg tgaggcccga gaagatgatc gcgcggccgg cggtccccat cgtccgcgcg
      961 agcgcgtctt cgacgtcacc atggcggagc tcttcccgga agcgcgccac gacgaagagc
     1021 gagtagtcga tggccgttcc caggccgatg agcgtgacga tgttgagcga atagggcgac
     1081 acgtccatga ctcgcgagag aaggaatacg ccggcgatcc cggcgacgat cgcgaggaga
     1141 cccacgccca gcggcaggag cgcgccgacg agcgtgccga acaccagcag cagcaggacg
     1201 agcgcgatgg ggagcgcgac gacctcggcg cgccgaaggt cggcgtcgag gatccggttc
     1261 agatcgtgat tgatcggcag attgctcgtg gcgtagatgc gcagcgtgtc cgagtggatc
     1321 tcgccgcgca ggtcgtcgta gtaatgatcc gcgaccgcgc ggaagtcacg gagcgtgacc
     1381 gacaccaccg tacgccggcc gtcgcgagag acgaggctcg cgctctgcgc gggcgcgtcg
     1441 taataggtgc gcatgctctg gacacgcgga tcggaccgga gcggcgcgac cgcatcgagg
     1501 acggcctcgc gatagcgggg ttcgtcgacg ctccggtcgt cgctctcgaa caccagcagg
     1561 aacgtcgtgc cgaccggcac ggcggcgctg gtcgcgcgcg gcagctgcgc gttgagcagg
     1621 ctcgaggcgc gtccggattc gcttgcgttc agcgattccg ggtcctgcag gtcgccaccc
     1681 tgcagcgcga cgacggcgct cgcggccagg agcacggcgg agacgagcag caggatccag
     1741 cgccgccggt agacgagcct tccccaccgc tccagcatcc cgtcctccgt acgctccgcg
     1801 gcacccgcgc gttccgtgtg cgtcaccgtt gacgttgacc gggccacgtc cgcccgtcat
     1861 cgtgaccgcc tggtgtcagc atcggacgtc ctgagcgcgc tcgccgagcg gctcccgcgc
     1921 ggctatcgcg cgcggccctt cgaggacgcg gaccgcgagc cgctcgtcgc cgcgggcaac
     1981 tccgaggcgc atcccatgga gcaggagacg gctgccgagt ggcgccgctg ggaatcgatg
     2041 ttcgcggacg acacgcgcgt acgggtcgtc gtggtctccg acgctggcac cgtcgtgggg
     2101 agcggcaacg tccaggccgg ctggacgcca cggcccgacg gcgcgcaaca cctgaacgtc
     2161 ggcgtacacc ccgagcaccg ccggcgcggc atcggcggcg cgctcctcga cgcgctcgag
     2221 gccgaaggcc accgccgcgg ggtgccgcgg ctcctgtccg gcgtctcgtc ggcgcgcccg
     2281 ttcgccgtcg cgttcgcgac gaagcacggc tatcgcgaga tcggccggcg gatcatgtcg
     2341 tacgtcgagc tcgacgcgtt cgagcccgac cgctggcgcg agtcggtcga tcgcgtggaa
     2401 cgcgagggca tccggctgcg gccgatcgcc gaagtgctcg ccggcctcga tgaagccggc
     2461 gccgaacgct tctggcgcgc cgtgtgggag gcggaggcgc cgatgtggga ggacatcccc
     2521 ttctcgtccc cgacgccgca ctgggcgttc gagcgcttct acaagttggc cgcgaagagc
     2581 gggcagctgc tcgccgacgt gagcgtcgtg gcgtacgccg gcgagcgcat cgtcggcttc
     2641 acggagaccg gcgaccgtca gggcaaggac ggctggacct ggatgaccgg cgtcgcgcgc
     2701 gaccaccgcg gaaagggcat cgcgatgggc ctcaaggtcg ccgcgctcac gctggcgaag
     2761 aagaagcgcc tgcgcgcgat gtgcaccgtc aacgacgagc cgaacaaggc gatgcgcggc
     2821 gtgaacatca agctgggcta tcagcctgtg ccggaccata tcgagctgga gaaacgtctc
     2881 tcggcaggag caacagcacc gctccgctga ggagcaccgc gaacgacgac agcagcagtc
     2941 catagcccac gtcgaacgcc tgccacagca gcgaagcgcc catcgcgccg atgacgacgc
     3001 cgccattcgc gatcgcgttg accgtcccga acacgcggcc gcgccgctcc ggcgcgacgg
     3061 cggtcgtgag cagggcgaac caggacgcgt tgaatgccgt gagcgacgcg ccggtgatga
     3121 ggctgatcgc gtacacgacg gggcgcggga ggccgaagac gagcgcgagc ccgaagaagc
     3181 acaccgggac cgacgccgcg atggccatgc gccggcggcc ggtgcggtcg gccaggtggc
     3241 cgagccacgg tccgagggcg atgtccccca gcccaaagat cgtgaagatg atcccgacgt
     3301 cgaaggtgct ccacccgagg ttcgccgtcg cgaagggtgc gtagctggtg atccatccgc
     3361 cgaagccgac gaggagcagc atgttcgccg cgaacaggac gagcacggcg cgcgtccagg
     3421 tgggctcgcc ggccggacga ggcgccgttg ccggccgctc tggccgcggc aggagggtgg
     3481 cgccgaggag ggcgatcgtg ctcgtcgccg caacgatcat gaacggcaca cgcaggtccg
     3541 ccgcgacggc gatgagcccg ccgaccgcgg ggccgaacac gttgcccgcc gaggtcgcag
     3601 cggacaggat gctgttgaag aacgcgagac gcttcggatc ggccgtctgc gtgatgtaga
     3661 gccgcgtcgc gatgatgttc gacccgccgc ccagaccggc gaggccgcgg tacgcgatga
     3721 gcgcgaccgc cgacgtcgcc gtggcgatga gcgcgttggc cgcggcgtac agcgcggtgc
     3781 cgccgatgat gaagcgcctc gatccgaaac ggtcgacgaa gaggcccccg ccgaactgcc
     3841 cgacggagtt cgcgaccgcg aacgcggagg tcagcagccc gagctgcagc ggcgtcgcgc
     3901 cgagcgacag cccgtacagg ggcagcagcg gcagcatgat cgagatgccc aggctcgaca
     3961 cgaacgagat cgcggccagc acgggcagcg cggggcccag cgtacggatg cgtccgagca
     4021 ccgcccgagc gtagggccac cgctgacatc agactgcgtc cggcgcgaca taccctgtca
     4081 ccgtgcccga tgcggcgatc gcgttcgaag gtgccagtaa gcgctaccgc agcggcacgc
     4141 tcgccctcca cgaggcgacc tggtcgatcc ccgtcggttc gcgctcgtgc ctgctcggcc
     4201 cgaacggctc ggggaagacg acctcgatcc gcctgctcga gggagcgctc acgccgacga
     4261 gcggatacgt ctcgctcctc gggaccacgg tcaacggcga cggctacgcc ctcgcccgcg
     4321 gccgctgcgg catcgtcccg caacaggccg gcatgtattc ggacctcacc acgtgggagt
     4381 acctcgagct cgcccgccga ctctacggtc gcggcagcgt cggcgatgcc ctggagcgtt
     4441 tcgggctgac cgagcatggc gacaagcggc tcgcgcagct ctcgggtggg ttccagcgca
     4501 gggtggtcgt ggcggccgcg ctcctgtccg agcccgacgt gctcctgctc gacgagccca
     4561 cggtcgggct cgatcccgtc gcggcgcgcg agatccacga cacgctgcgt gcggtgatgg
     4621 ccggtcggac gacgctcctc tgcacgcaca acctggccga ggcggaggcg ctctgcgacg
     4681 acgtgatcat cctccgcggc ggcgaggtcc tgctgcacga gccgctcgcg gagctgcgcc
     4741 agcgccagca tgcgcgaacg cggttcgcgg cgcggcagcc tgcggagacc ctcgcggcac
     4801 gggtgcgcgc gcacggcgcg atcgccacga tcgaggacgg ggcggtcatc gtcccgatcg
     4861 atgcgcgccg cgacgcaccg gcgctgctgc gcgcgctgct gcacgacggc atggacgtct
     4921 atgagtgcgc cccgctggag gcgaccctgg aggagctgtt cctcgaggcg gtgcggcgat
     4981 gaggatgagc gcgatccgtt cgctcctctg gaaggagacg cggcagctgg ggcgcaaccg
     5041 cacggcgatg ctgactgcgg cgctgctccc gttcctgatc ctcttcctcg cgccgatcca
     5101 gatgatcgtc ggcgtcaagc tcggcggcga ccgcgggatg gatcagctcc gcgaagcgcc
     5161 gcttcccggc ttcagcgacg tgtccgaccc cggccagctg ctcgtccagt tcttctaccc
     5221 gatgctgctc gtcatcggcg ggctcctcct gccctcgctc acgacgacct acgcgatcgt
     5281 gaccgagcgg gagcgccgct cgctcgagct gctcgtctcc ctcccggtga ccgtcgggga
     5341 gatcgtcgcc gcgaagctgc tctcggtcct cgtggtcacg gcgatgatcg ggatccccta
     5401 cgtcgccgtc gtgatcacgc tgctgatcgt cctcggcctg gccggcccgg caacggtccc
     5461 ggcgctgctc gcgccgttcg tcgcggcggt cgcgtgctcc acggcgatcg cgctgctgct
     5521 caccctgctc gcgcgcgact tccgcaccgc gaacaacctc aacggggcgt tcttcgtgcc
     5581 ggcgctcctg ctgagcatcg gcacgctcgc cggcgtgggc gggcccgcac gaacgtacgt
     5641 gctcgccggc gtcctgctcg cgctcgcggc gatcgcgctc ttcgcggccc tgcgctgggt
     5701 gtccttcgag cgctacctcg attagccaac ctcgcggagc gcgcgcaccg actggctgag
     5761 gaacgggtgc cgcgagagac gcgagaggga ggcccgcagg cctccctctc gtcggtgacc
     5821 ggccacgaga cctactcggc cggagtggcc tccgtgaccg cggccggctg ttcgccgccg
     5881 acctcgtgcc agatcttgac gccgcgcggc tcgcgcagga agagcgtgcc gaccacgacg
     5941 gtcatgagcg cgacgatcat gacgtaggcg agccccaggt agatgttgcc gttgggatcg
     6001 ccggtcgtgt tgaactgccg caggctgatg tccgtcttga tcggcagtcc catgaagttc
     6061 gtcaggaacg ggacgtagga cttgctgccc tccgggaacg tcgcgccgac gagacccgtg
     6121 tacaggagcg gcagaaggcc gccgaaccat ccgttcccga agtggtacgg gatcgagagc
     6181 gacgtgtagc ggaccttggc gcggaagtac tccacgagga atgccgcgat cgggccgtac
     6241 accatcgtca cgtagatgac ctggatccac accaggaaga cgaggagcgg gacgttggga
     6301 tcggccttcg tgatgaggcc ggtcgcgtca cgcgtcacgt tcgccgcgtc cgtcatggcg
     6361 tgatagatgg gcaggtacgt gaccgcggcg atgaggcagc ccagcaggat gatcggcttg
     6421 cggccgacct tgtcggagag ccatccgaac accaggaaga acggcgtgcc cagggcgacg
     6481 gcccagagca tgatcggata cgactgcgtg tagctgagct tcaggtacgt ggtcatgaag
     6541 aagttcgcct ggaactgtcc ctggtaccag accacgccct ggccggccgt gaggcccagc
     6601 agcacgaggg cgatcgtccc gaccttgcgg ccgctgaagg actcacgcgc ccagtgcccg
     6661 gtcgagcgga cgtgctcgtt cgcctccttg aggcgggcga acagcggcgt ctcgcggagc
     6721 cggatgcgga tgtacagcgc gatcgcgacg aggacggcgg agatccagaa gggatagcgc
     6781 cagccctgtg cggtgaagtc cgcagcgctc atgttcgtgc ggaagaacag gatgatcagg
     6841 agcgcgagga agaagcccac cgtcgcggtc gtctggatcc acgacgtgta cagaccccgc
     6901 ttgttgtcgg gtgcgtgctc ggccacgtag atggccgcgc cgccgtactc accaccgagc
     6961 gcgaggccct ggatgaggcg cagcgccacc aggatgaacg cggccgtgac gccgatgtcc
     7021 ttgtacgtgg gcaggaagcc gatgatcacc gtcgagagac ccatcgcggt gatcgtgacg
     7081 aggaacgtgt acttgcggcc gatgatgtcg ccgagcgcgc cgaagaacac ggcgccgaac
     7141 g
//
LOCUS       DASTZR010000295         2953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_65020, whole genome shotgun sequence.
ACCESSION   DASTZR010000295 DASTZR010000000
VERSION     DASTZR010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65020"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1569)
                     /locus_tag="VFV20_06650"
     CDS             complement(<1..1569)
                     /locus_tag="VFV20_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5288064.1"
                     /translation="MGLSAPVERSKDFKGTLRKLLRRLRPEWTTIAIVAVIGAASVAF
                     TVIGPKIVGNAMNVIFDGVIGSSLPAGLTKDQAVAALRARGQGQLADVVSGSGAVPGQ
                     GIDFTALEQILLIAAVVYVLGSLLAWLQGYLMAGVAQRTVYGLRREVEEKLARLPLRY
                     FDSHAHGDLLSRVTNDVDNIATTLQQGLSQLMTSILTVVGVLAMMLWISPLLALISIL
                     TIPLSFFVTIVIAGRSQKEFVAQWAETGALNGHVEQMHSGHALVQVFGRRRSALEQFM
                     TQNARLYDAAFRAQFLSGIIQPAMFFLSNLNYVGIAVIGGYRVASGAMTLGDVQAFIQ
                     YSRQFTQPLAQIAAQMNLLQSGLASAERVFEFLGAEELTPERVPGAVLPAVRGHVELD
                     NVSFRYLPERPLIEDFSLDVRPGETVAIVGPTGAGKTTIVNLLMRFYEIDGGAIRLDG
                     VDTRDLPRDELRRAFAMVLQDSWLFVGTIRDNIAYGREGASLEEIVAAATAAHVDAFV
                     RTLPDGYDTVLEEEA"
     gene            complement(1632..>2953)
                     /locus_tag="VFV20_06655"
     CDS             complement(1632..>2953)
                     /locus_tag="VFV20_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5288065.1"
                     /translation="SAPILAIGGVIMAVREDATLSWLLVVVVPLMALVIGSLLARAVP
                     LFRAVQAKIDTITRVMREQITGVRVVRAFIRVGSERRRFEVANADLTDTSLRVNRIFV
                     LAFPSLLGIMNLSTVAILWFGAQLIDSGDMPIGNLAAFMQYIFQILFAVMAAVFMVIL
                     IPRAEASSQRIIEVLDTLPSIAEPEHPVTPDRPSGEVAFRDVTFAYPHSERPVLHDIS
                     FVARPGATTAIIGGTGSGKTTVVNLVTRAFDATGGSVAVDGVDVREQALERLWGQMAL
                     VPQTAYLFKGTVAENLRFGGPEATDEDLWRALEIAQARDFVAAMKGQLEASIDQGGTN
                     VSGGQRQRLAIARAIVKRPAIYLFDDCFSALDAATDARLRAALRRQTADAAVIIVAQR
                     VSTILHADHIVVLDEGRVVGSGTHADLMRSSAQYREIVESQLGASAA"
BASE COUNT          482 a          990 c         1065 g          416 t
ORIGIN      
        1 cgcctcctcc tcgagcaccg tgtcgtagcc gtccgggagc gtgcggacga acgcgtcgac
       61 gtgcgcggcc gtcgcggcgg cgacgatctc ctccagtgag gcgccctccc tgccgtacgc
      121 gatgttgtcg cggatcgtgc ccacgaagag ccacgagtcc tgcagcacca tggcgaacgc
      181 gcggcgcagc tcgtcgcgcg ggaggtcgcg tgtgtcgacc ccgtcgaggc ggatcgcgcc
      241 gccatcgatc tcgtagaagc gcatgagcag gttcacgatg gtcgtcttgc ccgcgccggt
      301 cggaccgacg atggccacgg tctcgcccgg gcggacgtcg agcgagaagt cctcgatgag
      361 cggcctctcg ggcaggtagc ggaacgacac gttgtcgagc tccacgtgtc cgcgcaccgc
      421 gggcaggacg gccccgggca cgcgctccgg cgtgagctcc tcggccccaa ggaactcgaa
      481 gacgcgctcc gccgacgcga ggcccgactg gagcaggttc atctgcgcgg cgatctgcgc
      541 gaggggctgg gtgaactgcc gcgagtactg gatgaacgcc tgcacgtccc cgagggtcat
      601 cgcgcccgag gcgacgcggt acccgccgat gaccgcgatg cccacgtagt tcaggttgga
      661 gaggaagaac atcgcgggct ggatgatccc ggagaggaac tgcgcgcgga aggccgcgtc
      721 gtacaggcgc gcgttctgcg tcatgaactg ctcgagggcg ctgcggcggc gcccgaagac
      781 ctgcaccagc gcgtgcccgc tgtgcatctg ctcgacatga ccgttgagcg cgccggtctc
      841 ggcccactgg gcgacgaact ccttctggga ccggccggcg atgacgatcg tcacgaagaa
      901 cgagagaggg atggtgagga tcgagatgag cgcgaggaga ggactgatcc acagcatcat
      961 cgcgagcacg ccgaccacgg tgaggatcga ggtcatgagc tggctgaggc cctgctggag
     1021 cgtcgtcgcg atgttgtcca cgtcgttggt gacgcgggag aggaggtcgc cgtgcgcgtg
     1081 gctgtcgaag tagcggagcg gcaggcgcgc gagcttctcc tcgacctcgc gacgcaggcc
     1141 gtagaccgtg cgctgcgcga cgccggccat gaggtagccc tggagccacg cgaggagcga
     1201 gccgaggaca tagacgacgg ccgcgatcag gaggatctgc tccagagcgg tgaagtcgat
     1261 gccctgcccc gggaccgcgc ccgatcccga cacgacgtcg gcgagctggc cctgcccacg
     1321 agcgcgcagc gcggcgacgg cctggtcctt cgtgaggccg gcagggagcg agctgccgat
     1381 gacgccgtcg aagatgacgt tcatcgcgtt gcccacgatc ttcggaccga tgacggtgaa
     1441 cgcgacgctc gccgcgccga tcaccgcgac gatcgcgatg gtggtccact ccggacggag
     1501 acggcggagc agcttgcgca gcgtgccctt gaaatccttg gagcgctcga ccggtgccga
     1561 gagtcccatg aaaccacccg ccgcgggtcc gcctggacgc ccgccgggac cgggacgccg
     1621 gcccatgacc atcatgccgc cgacgctccc agctgcgact cgacgatctc gcggtactgc
     1681 gccgatgacc gcatgaggtc ggcgtgcgtg ccggagccga cgacgcggcc ttcgtcgagc
     1741 acgacgatgt ggtccgcgtg caggatggtg gagacgcgct gagcgacgat gatgacggcc
     1801 gcgtcggcgg tctggcgccg gagcgcagcc cgcagccgcg catcggtggc cgcgtcgagc
     1861 gccgagaagc agtcgtcgaa caggtagatc gccggccgct tcacgatcgc gcgcgcgatc
     1921 gcgaggcgct ggcgttggcc cccggacacg ttcgtcccac cctggtcgat ggacgcctcg
     1981 agctggccct tcatcgcggc gacgaaatcg cgcgcctgtg cgatctccag tgcgcgccag
     2041 aggtcctcgt ccgtcgcctc cgggccgccg aagcgcagat tctccgcgac ggtccccttg
     2101 aacaggtacg cggtctgcgg gacgagcgcc atctggcccc agagccgctc gagcgcctgc
     2161 tcacgcacgt cgacgccgtc caccgcgacg ctgccgcccg tcgcgtcgaa ggcgcgcgtg
     2221 acgaggttca ccacggtggt cttccccgat ccggtgccgc cgatgatcgc cgtggtcgcg
     2281 ccgggccgcg cgacgaacga gatgtcgtgg aggaccggtc gctcgctgtg cggataggcg
     2341 aaggtgacgt cgcggaaggc gacctcgccg ctcggacggt cgggcgtcac gggatgctcc
     2401 ggctcggcga tggacggcag cgtgtcgagc acctcgatga tgcgctgcga gctcgcctcg
     2461 gcgcgcggga tcaggatgac catgaacacc gcggccatga ccgcgaacag gatctggaag
     2521 atgtactgca tgaacgcggc gaggttcccg atgggcatgt cgccgctgtc gatgagctgc
     2581 gcgccgaacc acaggatcgc gaccgtcgac aggttcatga tcccgagcag gctcgggaac
     2641 gccaggacga agatgcggtt cacgcgcagg ctggtgtcgg tgaggtcggc gttcgcgacc
     2701 tcgaagcgcc gccgctccga tccgacgcga atgaacgcac gcacgacgcg gaccccggtg
     2761 atctgctcgc gcatgacgcg cgtgatggtg tcgatcttcg cctgtacggc gcggaacagc
     2821 ggtaccgcgc gcgcgaggag gctcccgatg acgagcgcca tgagcggcac gacgacgacc
     2881 aggagccacg agagcgtggc gtcctcccgc acggccatga tcacgccgcc gatcgccagg
     2941 atcggcgcgc tga
//
LOCUS       DASTZR010000296         3359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_748076, whole genome shotgun sequence.
ACCESSION   DASTZR010000296 DASTZR010000000
VERSION     DASTZR010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748076"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VFV20_06660"
     CDS             complement(<1..596)
                     /locus_tag="VFV20_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU5288066.1"
                     /translation="MIDNPRSPRVRGVAKLAKRDARSETGLFLLEGPQAVSEALAYRP
                     ELLIELFVTPTALDRYSELGRAAREAGLDVEFVTEQVIDAMADTVTPQGVIAVCRQFP
                     TSLKDILGDGPRLIAILEEVRDPGNAGTIIRAADSAGADAVILTGRSVDLYNPKVVRA
                     TTGSLFHVPVAVMPDLDAVLQRVKDAGLQVLAADIKGE"
     gene            complement(606..992)
                     /gene="rplT"
                     /locus_tag="VFV20_06665"
     CDS             complement(606..992)
                     /gene="rplT"
                     /locus_tag="VFV20_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU5288067.1"
                     /translation="MARVKRAVNAHKKRRVILERAKGYRGQRSRLYRKAKEQVTHSLV
                     YAYRDRRARKGDFRRLWIQRINAAARANGLTYNRFIQGLNLAGIEVDRRILADLAITE
                     PATFAALVASAKAALPANTSAPKVTA"
     gene            complement(1020..1214)
                     /gene="rpmI"
                     /locus_tag="VFV20_06670"
     CDS             complement(1020..1214)
                     /gene="rpmI"
                     /locus_tag="VFV20_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU5288068.1"
                     /translation="MPKQKTHSGAKKRFKVTGTGKIKKQTAGMRHNLEVKSGKKKRDL
                     NQDHVIAAADAKGIKKLLGK"
     gene            complement(1537..2121)
                     /gene="infC"
                     /locus_tag="VFV20_06675"
                     /pseudo
     CDS             complement(1537..2121)
                     /gene="infC"
                     /locus_tag="VFV20_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202430.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
     gene            2338..2685
                     /locus_tag="VFV20_06680"
     CDS             2338..2685
                     /locus_tag="VFV20_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HEU5288069.1"
                     /translation="MSDYSYVDDDTRDISEVPAVEVIQTVSVHLMTAAAVKLGLADDP
                     NAADQVDLDEARTLIEALAGLVTASAPKIGDHHALALRDGLRTLQLAFREESSHPDAP
                     GKGPGEKFTGPVN"
     gene            complement(2696..>3359)
                     /locus_tag="VFV20_06685"
     CDS             complement(2696..>3359)
                     /locus_tag="VFV20_06685"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU5288070.1"
                     /translation="GLTELAAVSDVVVAYPNGTAANAVAGELAWNAGGCCGLASSGNV
                     DDVGFVLAMIADLQQTYPIDASRIYLAGYSNGGMMSYRLACEHAELFAGIAVVSGALN
                     YSPCEPSRPLSVLIVHGTGDLTVPYDGGETNARTAARFGQWFNTPVEFATAFWTENDD
                     CDAQAQRTGSPPITTDVYPDCADGTRVEVVTIDRGTHTWPRTDSLGVDGAELVLDFFG
                     LL"
BASE COUNT          474 a         1090 c         1184 g          611 t
ORIGIN      
        1 tcctccccct tgatgtcggc ggcgagcacc tgcaggccgg cgtccttgac gcgctgcagc
       61 acggcatcca ggtctggcat gacggcgacc ggcacgtgga agagcgaccc cgtcgtcgcc
      121 cgcacgacct tggggttgta gaggtcgacg ctgcgcccgg tgaggatgac ggcgtcggcg
      181 cccgcggagt ccgcggcccg gatgatcgtg cccgcgttgc cggggtcgcg cacctcctcg
      241 aggatcgcga tcaatcgcgg gccgtcaccg aggatgtcct tgagcgaggt cggaaactgc
      301 cggcagaccg cgatgacccc ctgcggcgtc accgtgtcgg ccatcgcatc gatgacctgc
      361 tcggtgacga actccacgtc gagccccgct tcgcgcgccg cccggccgag ctcgctgtag
      421 cggtcgaggg cggtcggcgt gacgaagagc tcgatgagca actcggggcg ataggccagg
      481 gcctcggata ccgcctgcgg gccctccaaa agaaagagcc cggtctcaga ccgggcgtct
      541 ctcttggcaa gctttgccac gccgcgcact cggggagaac gcgggttatc aatcacagtg
      601 ctactttacg cggtcacctt cggtgcgctg gtgttcgcgg gcagcgcggc cttggccgat
      661 gcgacgaggg ccgcgaaggt ggcgggctcg gtgatcgcga ggtcggcgag gatgcggcgg
      721 tcgacctcga tgccggccag gttgaggccc tggatgaagc ggttgtaggt gaggccgttg
      781 gcacgggcag ccgcgttgat gcgctggatc cacaggcgac ggaagtcgcc cttgcgggcg
      841 cggcggtcgc ggtaggcgta gacgagcgag tgggtgacct gctccttggc cttgcggtag
      901 aggcgcgaac gctggccgcg gtatcccttg gcgcgctcga ggatgacgcg acgcttcttg
      961 tgagcgttga ctgctctctt tactcttgcc atttcacttg ttccttagta gtcgttcggc
     1021 tacttgccga gaagcttctt gatgcccttg gcgtcggccg cggcaataac gtggtcctgg
     1081 ttcagatcgc gcttcttctt gcccgacttg acttcgaggt tgtgacgcat accagcggtc
     1141 tgcttcttga tctttccggt gccggtgacc ttgaagcgct tcttggcgcc ggagtgggtc
     1201 ttctgcttgg gcatgtctcg ttccttactt tttggtctcg cccgtgggcg cagccggctt
     1261 ggcagcaggc ttcgcgggtg cgggggtctt gggggccgcc ttggccgcgg caggcttcgc
     1321 tgccgcgggc ttcgacggag tagcgggggc gaccggcttc tcggccaccg gggcggccgg
     1381 cttgccgtcg acggcggggg ccttcggcgc gacggccttc ggcgcgggag cgtcggcctt
     1441 gacgacggcc ggggcagccg cgggtgcggc ttcggccttc ggtgcggcgg gggcctcggc
     1501 cgcgggcttc gcgaggggcg tgcgggcagg cttcgcggcg gcgcgcttgg cgtcggcctc
     1561 ggccttcgcc gtcgccttgc tcttgtgcgg gccgatgacc atgaccatgt tgcggccgtc
     1621 gatggtcggc gtcgactcga cggagccgaa ctcggcgaca tcctcggcga agcgctgcag
     1681 caggcggacg ccctgctcgg ggcgcgactg ctcgcggccg cggaacagga tcatggcctt
     1741 caccttgtcg ccggccgcga ggaatccctc ggcgtgcttg cgcttggtct cgtagtcgtg
     1801 cgtatcgatc ttcaaacgga aacgcacctc cttgagaatg gtgttcgcct gattgcggcg
     1861 tgcctctttg gccttctggg cctcttcgta cttgaacttg ccgtagtcca tgatcttggc
     1921 gacgggcggc ttggaattgg gagcaacctc aaccaaatcc aggtcggcct cctgcgccaa
     1981 gcggagggcg tcctcgatgc ggacgactcc tacctgctcg ccgttggggc caacaagacg
     2041 gacctccgga acgcggattc ggtcattggt acggggatcg ctgatgcgag tcttctttcg
     2101 ttgagcttcg tgagcctgca tgccgacgga tgccgcgcag cagcgaaagg aaaagatttc
     2161 aatgcaccct ttgtgtctac cggtcacgcg aatgcacgcg atctggcggg gtgtacttac
     2221 ccggtaacct ttatgagtcg gttgccaggt gggagaatct ccactttcgt accggggaca
     2281 cgccccggag cctgcataag agtagcagat accgccacat tcacgaaggg ctcccccatg
     2341 tcggactaca gctatgtcga cgacgacacc cgcgacatct ccgaggtgcc cgcggtcgag
     2401 gtcatccaga cggtgtccgt gcacctcatg accgccgccg ccgtcaagct cggcctcgcc
     2461 gacgacccca acgccgcgga ccaggtggac ctcgacgagg cccgcaccct catcgaggct
     2521 ctcgccggcc tcgtcaccgc gagcgccccc aagatcggcg accaccacgc cctcgccctg
     2581 cgcgacggcc tgcgcacgct gcagctggcc ttccgcgagg agtcgtcgca ccctgacgcc
     2641 ccgggcaagg gtccgggcga gaagttcacg ggaccggtca actaacgacc ccgggctaca
     2701 agagcccgaa gaagtcgagc acgagctctg ccccgtcgac cccgagcgag tcggtgcgcg
     2761 gccaggtgtg ggtgccgcgg tcgatcgtca cgacctcgac gcgggtgccg tctgcgcagt
     2821 cggggtagac gtcggtcgtg atcggcgggc tgccggtgcg ctgcgcctgg gcgtcgcagt
     2881 cgtcgttctc cgtccagaag gccgtcgcga actcgaccgg tgtgttgaac cactggccga
     2941 agcgcgccgc ggtgcgcgcg ttcgtctctc ccccgtcgta gggcacagtg aggtcgccgg
     3001 tgccgtgcac gatgagcacc gacaggggcc ggctcggctc gcagggcgag tagttgaggg
     3061 ccccgctgac gacggcgatg ccggcgaaga gctccgcgtg ctcgcaggcg aggcggtagc
     3121 tcatcatgcc gccgttgctg tagccggcga ggtagatgcg gctggcatcg atggggtagg
     3181 tctgctgcag gtcggcgatc atcgcgagca cgaagccgac gtcgtcgaca ttgccgctgc
     3241 tcgcgaggcc gcagcagccg ccggcgttcc aggcgagctc gcccgcgacg gcgttcgccg
     3301 cagtgccatt cgggtaggcg accaccacgt cgctgacggc cgccagctcg gtgaggccg
//
LOCUS       DASTZR010000297         2218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_304191, whole genome shotgun sequence.
ACCESSION   DASTZR010000297 DASTZR010000000
VERSION     DASTZR010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304191"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="VFV20_06690"
     CDS             <1..978
                     /locus_tag="VFV20_06690"
                     /inference="COORDINATES: protein motif:HMM:NF025877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HEU5288071.1"
                     /translation="LRIVSVIITPTSGRVVAGGRVASLLELGAGFHPDFTGRENVYLN
                     ASLLGLSRAQTERVLPEIIAFAELGDFFDAAVKTYSSGMYMRLAFSVAAFVDPDVLLI
                     DEILAVGDEYFQRKCYAKLNEFRSRKKTICFVSHDLAAVERLCRRGLLLQNGVVRAEG
                     DIQRVLEAYHELVDAQEEGRLAGEGPRGDRWGSGEVTIEEVTLHSGAEATHVVRSGAP
                     FEIRLAYRAAEERDAVFGVLIYRDDGLSVYGTNTKNDAVPIRAKREGHVSFCVDALPL
                     LPGKYELDVAASSPDLHAYDYHSKRYTFRVVGSAGEMGTARLDHHWEAS"
     gene            1035..1325
                     /locus_tag="VFV20_06695"
     CDS             1035..1325
                     /locus_tag="VFV20_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEU5288072.1"
                     /translation="MARVNVRELARNTSKVIDDVTHRKQATLVTRDGLPVAALVPIDS
                     EALEDWILANAPEFVEGMRLADEEARRGDTVSFDDLLAKPKRKTTRRARARR"
     gene            1624..>2218
                     /locus_tag="VFV20_06700"
     CDS             1624..>2218
                     /locus_tag="VFV20_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288073.1"
                     /translation="MTDTVDLDAILTEVRERVRRKRDRGIYGSDVEALLRTELPGGRR
                     LLSDDMKDPVGALAEALDEDAVYDPRSHKRVVGPLITFARRTLIGLLRWWMAAVIDRQ
                     ERINRLLAATYDVEGRLAPRFGERLARLEREWQQWREHEIAANLHSVYFQARFGGDES
                     VIRTQSEQFVDLFAGRSRVLDLGCGRGIFLGLLRDRGV"
BASE COUNT          349 a          749 c          762 g          358 t
ORIGIN      
        1 ctgcggatcg tctccgtgat catcacgccg acgtccggtc gcgtcgtagc gggcggtcgc
       61 gtcgcgtcgc tgctcgagct cggagccggc ttccatcccg acttcaccgg gcgcgagaac
      121 gtctacctca acgcgtcgct cctcggtctc tctcgcgcgc agaccgagcg cgtgctcccg
      181 gagatcatcg cgttcgccga gctcggcgac ttcttcgacg ccgcggtgaa gacgtactcg
      241 tccggcatgt acatgcgcct ggcgttctcg gtcgccgcgt tcgtcgatcc ggacgtgcta
      301 ctgatcgacg agatcctcgc cgtgggagac gagtacttcc agcgcaagtg ctacgcgaag
      361 ctcaacgagt tccgctcccg gaagaagacg atctgcttcg tgtcacacga cctcgccgcg
      421 gtcgagcggc tctgccgccg cggtctcttg ctgcagaacg gcgtcgtgcg cgccgagggc
      481 gacatccagc gcgtgctcga ggcctatcac gagctcgtcg acgcgcagga ggagggccgc
      541 ctcgccggcg agggtccgag gggcgatcgc tggggcagcg gcgaggtcac gatcgaagag
      601 gtgacgctcc actcgggcgc cgaggcgacg cacgtggtgc gctcgggggc cccgttcgag
      661 atccgcctcg cgtaccgggc agcggaggag cgcgacgcgg tgttcggcgt cctcatctac
      721 cgcgacgacg ggctgtcggt ctacgggacg aacaccaaga acgatgccgt gccgatacga
      781 gcgaagcgcg agggccacgt ttccttctgc gtggacgcgc tcccgctgct gccggggaag
      841 tacgagctgg acgtcgcggc gtcgtcgccc gacctccatg cctatgacta ccattccaag
      901 cgctacacgt tccgcgtcgt cggatccgcg ggcgagatgg gaacggcgcg cctagatcac
      961 cactgggagg cgtcgtgagg accactcccg tcggctacaa tgtctgacga acgcatgaca
     1021 gcaaggagtc caggatggcg agagtgaacg tacgcgagct tgcgcggaac acgagcaagg
     1081 tcatcgatga cgtcacgcat cggaagcagg ccacgctcgt gactcgcgac ggcctgccgg
     1141 tcgccgccct cgtgccgatc gactccgaag cgctcgagga ttggatcctc gccaacgcac
     1201 ccgagttcgt cgaaggcatg cggctggcag acgaagaagc gcgtcgtggc gataccgtgt
     1261 cgttcgatga tctcctcgcg aagccgaagc ggaagacgac ccgtcgtgct cgcgcgcgtc
     1321 gttgagagcg gcgctccgcc ttacgaagcg tgccgagcat gattggcaga agctcgatgc
     1381 cgctggccgg gaacgcgtgg agcttgcact ggaagcgttg cgggcgatac cgcgcccggc
     1441 gaatctggat gagaagcccc tcgagggtgc tccaccttgg cgaaggcttc gcgtgggcga
     1501 gcggcgcatc atctttcgtc ccttcacgaa ggaggagagc cgggtgtacg ccgccgacag
     1561 tggcttcatc gtggagcgtg tcgtcgacag gcgggacctc gatcgcgccg tgaagtccct
     1621 ccgatgaccg ataccgtcga cctcgacgcg atcctcaccg aggtacgcga gcgcgtccgc
     1681 cgcaagcgcg accgcggcat ctacggctcc gacgtcgagg cgctccttcg caccgagctt
     1741 cccggtggcc ggcgtctcct ctccgacgac atgaaggatc cggtcggcgc gctcgcggag
     1801 gcgctcgacg aggacgccgt ctacgacccg cggagccaca agcgcgtcgt cggcccgctc
     1861 atcacgttcg cgcgccgcac gctcatcggg ctcctgcgct ggtggatggc ggcggtcatc
     1921 gaccggcagg agcgcatcaa ccggcttctt gcggcgacgt acgacgtcga gggacgcctc
     1981 gcgccgcgct tcggcgagcg cctcgcgcgc ctcgagcgcg aatggcagca gtggcgcgag
     2041 cacgagatcg cggcgaacct gcactcggtc tacttccagg cgcgcttcgg tggcgacgag
     2101 tcggtcatcc gcacgcagtc ggagcagttc gtggacctct tcgcgggccg gagccgcgtg
     2161 ctcgacctcg gctgcggccg cggcatcttc ctcgggctgc tccgcgaccg cggggtcg
//
LOCUS       DASTZR010000298         4194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_695537, whole genome shotgun sequence.
ACCESSION   DASTZR010000298 DASTZR010000000
VERSION     DASTZR010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695537"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1239
                     /locus_tag="VFV20_06705"
     CDS             <1..1239
                     /locus_tag="VFV20_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5288074.1"
                     /translation="GLTVGFLADLALKTLYLRGQMTMGEIASALGLAITGVTDRVMEF
                     LKGERLVEIRGGTGISSASYQFVIVDRGSEKAQEALARSQYVGKAPVPLATYIAAVQR
                     QSIANIHVTPEDLSKAFSHMVIPRETLAQLGPAVNSGKSIFLFGPPGNGKTTIAEVLV
                     SLLKGEVVLPYAVEVDQQVIKIFDQVYHRPALDPAAAERLRFDHRWVISKRPIVMTGG
                     ELTLETLDLIYDETSKFYEAPFQMKANGGIFMVDDFGRQRVSPKDLLNRWIVPLEKRV
                     DYLTLHTGKKVEIPFDMLIIFSTNLDPADLVDEAFLRRIRYKIGIEAPTVEQYEEIFK
                     RMCARKNIEYRAEAMSQILAIYRRKNLGLRSCHPRDILEQLIDTSRFLGQQPALTPQL
                     IDMACDSYFVSLDPSGAPKE"
     gene            1265..2368
                     /locus_tag="VFV20_06710"
     CDS             1265..2368
                     /locus_tag="VFV20_06710"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HEU5288075.1"
                     /translation="MGLGVLSDGLAPTPPMGWNSWNRFGPFISERLVLETADALVATG
                     MREAGYRYVVIDDAWHESLRDDDGDLVENRWAFPHGIAWLADEIHRRGLRFGLYTCAG
                     SRTCQGYPASRGCEARDAKRFARWGVDFMKVDWCHTAGLDAPSAYRTWCDAIRAAGRP
                     MVLSICEWGRHRPWEWAPSVGHLWRTSPDIADTWASLVATLDRQAELHPFAGPDHWND
                     PDMLEVGNGGMSDAEYRTHVSLWAMLAAPLMAGNDVREMRETTLAILTASEVLAVDQD
                     PLGRQARRVRVEGSTELWARELADGSRAALLLNRGEAAQRVWLTWDEVGLDPLAAMTV
                     RDLWGRSDVGELDEGYDATLASHAALLLKVSPS"
     gene            complement(2355..3167)
                     /locus_tag="VFV20_06715"
     CDS             complement(2355..3167)
                     /locus_tag="VFV20_06715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEU5288076.1"
                     /translation="MTDTGILARGSVARRLNRESFLLLGGTAALLLQVAHPLVAAGVA
                     QHSGFRREPFGRLLRTLDTTLAVVFGTTAEARAALRRIDRRHVAVHGTAADGRPYAAQ
                     DPDLLVWVQVTLVMTSLRLYELVMGPLADAERESYWQEARFFASELGATAGSLPATFA
                     DAARYEQDMLSRTVIPDATAREVARDVLHPFRWLPAWASWPLDAVTAGLLPEAPRTAF
                     GLRWRRRERLFFRGVIVALRGSVPLLPERLRFVPQARRYAARAAEAGGVRMD"
     gene            3186..3530
                     /locus_tag="VFV20_06720"
     CDS             3186..3530
                     /locus_tag="VFV20_06720"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEU5288077.1"
                     /translation="MRSFAYALSGLGYLLATERNFRIEVMAAALAVIAGTWLGLERLE
                     WLALALTIGLVLILEALNTAIEDAVSLASPGFDERARAAKDVSAAAVLVASLLAIVVG
                     LLLFGPRIIRVG"
     gene            3567..3902
                     /locus_tag="VFV20_06725"
     CDS             3567..3902
                     /locus_tag="VFV20_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006766110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5288078.1"
                     /translation="MAATGIHHVYLETHSFGKTAKFWQALGFVLDEDRGTSGLLRPPA
                     GGPYVYVAEVPPSQTPRTELYLNADDEAVPGRPVEVTAPWADTHWGTREMAIRDPDGR
                     TLKLESRGT"
     gene            3944..>4194
                     /gene="gatC"
                     /locus_tag="VFV20_06730"
     CDS             3944..>4194
                     /gene="gatC"
                     /locus_tag="VFV20_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU5288079.1"
                     /translation="MPIDRQTIVHVADLARIQLSDEETLRFTGQLSVVLDAVARLAEV
                     DTSAVLPSASVLPLRDVQREDESRPGLTNEQALANAPKGG"
BASE COUNT          648 a         1485 c         1451 g          610 t
ORIGIN      
        1 gggctcaccg tcggcttcct cgccgatctc gcgctgaaga cgctgtacct ccgcggtcag
       61 atgaccatgg gcgagatcgc ctccgcgctc ggcctcgcga tcacgggcgt gaccgaccgc
      121 gtcatggagt tcctcaaggg cgagcgcctg gtggagatac gcggcggcac gggcatctcg
      181 tccgcgtcct atcagttcgt catcgtcgat cgcgggtcgg agaaggcgca ggaggcgctc
      241 gcgcgcagcc agtacgtcgg caaggcgccc gtcccgctcg ccacgtacat cgccgcggtg
      301 cagcggcagt cgatcgcgaa catccacgtc acgccggagg acctcagcaa ggcgttctcg
      361 cacatggtca tcccgcgcga gaccctcgcg cagctcggtc ccgcggtgaa ctcgggcaag
      421 tcgatcttcc tcttcggacc gcccggcaac ggcaagacca cgatcgccga ggtcctcgtc
      481 tcactgctga aaggcgaggt cgtcctgccc tacgccgtgg aggtggacca gcaggtcatc
      541 aagatcttcg accaggtcta tcaccggccc gcgctcgatc cggcggccgc ggagcgcctc
      601 cgcttcgatc accgctgggt catcagcaag cggcccatcg tcatgaccgg cggtgagctc
      661 acgctcgaga cgctcgacct gatctacgac gagacgtcaa agttctacga agcgccgttc
      721 cagatgaagg cgaacggcgg catcttcatg gtcgacgact tcggccgcca gcgcgtgtca
      781 ccgaaggacc tcctcaaccg gtggatcgtg ccgctcgaga agcgcgtcga ttacctcacg
      841 ctgcacaccg ggaagaaggt cgagatcccc tttgacatgc tcatcatctt ctcgaccaac
      901 ctcgatccgg cggacctggt ggacgaggcg ttcctgcgcc gcatccgcta caagatcggc
      961 atcgaggccc ccacggtcga gcagtacgag gagatcttca agcgcatgtg cgcgcggaag
     1021 aacatcgagt accgcgccga ggcgatgagc cagatcctcg cgatctaccg gcggaagaac
     1081 ctcggcctgc gctcgtgcca cccgcgcgac atcttggagc agctcatcga cacgtcgcgc
     1141 ttcctcggcc agcagccggc gctcacgccg cagctcatcg acatggcctg cgactcctac
     1201 ttcgtttcgc tcgacccatc gggcgcgccg aaggagtagc gcggggccat ttaccatccg
     1261 cgcgatgggc cttggcgtcc tcagcgacgg cctcgcgccg accccgccga tgggctggaa
     1321 ctcctggaat cgcttcggtc ccttcatctc cgaacgtctc gtcctcgaga cggccgacgc
     1381 gctcgtcgcg accgggatgc gtgaggcggg ctaccgctac gtcgtgatcg acgatgcctg
     1441 gcacgagagc ctgcgtgacg acgacggtga tttggtcgag aaccgctggg cgttccccca
     1501 cggcatcgcc tggctggccg acgagatcca ccgccgcggg ctccggttcg gcctgtacac
     1561 ctgcgccggt tcgcgaacct gccagggcta tccggcgagc cgcggctgcg aggcgcgcga
     1621 cgcgaagcgc ttcgcccgat ggggcgtcga tttcatgaag gtcgattggt gccacaccgc
     1681 cggcctcgat gcgccgagcg cgtaccgcac gtggtgcgat gcgatccgcg ccgccgggcg
     1741 gccgatggtg ctctcgatct gcgaatgggg gaggcatcgg ccctgggagt gggcaccgag
     1801 cgttgggcat ctctggcgca cgagtccgga catcgccgac acgtgggcca gcctcgtggc
     1861 gaccctcgac cgtcaggccg agctgcatcc gttcgccggc ccggaccact ggaacgaccc
     1921 cgacatgctc gaggtgggga acggcggcat gagcgacgcg gagtaccgca cgcacgtctc
     1981 gctctgggcg atgctcgccg cgccgctcat ggccgggaac gacgtgcgcg agatgcgcga
     2041 gacgacgctc gcgatcctca ccgcctccga ggtgctcgcc gtcgaccagg atccgctggg
     2101 ccggcaggcg cggcgcgtgc gtgtggaagg ttcgaccgag ctctgggcgc gcgagctcgc
     2161 cgacggatcg cgcgcggcgc tcctgttgaa tcgcggcgag gcggcgcagc gcgtgtggct
     2221 gacctgggac gaggtcgggc tcgatccgct cgcggcgatg accgtccgcg acctgtgggg
     2281 ccgctccgac gtcggcgagc tcgacgaggg ctacgacgcg acgctcgcgt cgcacgcggc
     2341 gctgctcctg aaggtcagtc catcctgacg ccacccgctt ccgcggcgcg cgcggcgtaa
     2401 cgccgcgcct gcggcacgaa gcgaaggcgc tccggcagga ggggaacgct ccctcggagg
     2461 gcgacgatga ccccgcggaa gaagagccgc tcgcgccggc gccagcgcag gccgaacgcc
     2521 gtccgcggtg cctcgggcag cagacccgcg gtgaccgcat cgagtggcca gctcgcccag
     2581 gccggtagcc accggaacgg gtggagcacg tcgcgcgcga cctcccgcgc ggtggcgtcg
     2641 gggatcaccg tacgcgagag catgtcctgc tcgtaccgcg cggcgtcggc gaacgtggcg
     2701 ggaagcgagc cggccgtcgc gcccagctcc gaggcgaaga agcgcgcctc ctgccagtac
     2761 gactcgcgct ccgcgtccgc gagaggtccc atcacgagct cgtagaggcg cagcgaggtc
     2821 atcaccagcg tgacctgcac ccagaccagg agatccgggt cctgcgcggc atacggccga
     2881 ccgtcggcgg cggtcccgtg cacggcgacg tgacggcggt cgatgcgtcg cagcgcggca
     2941 cgcgcctcgg ccgtcgtgcc gaagacgacc gcgagcgtgg tgtcgagcgt ccggagcagc
     3001 cgcccgaacg gctcgcggcg gaagcccgaa tgctgggcga ccccggccgc caccagcgga
     3061 tgcgccacct gcagcagcag cgcggcggtg ccacccagca gcaggaacga ctcgcggttc
     3121 aggcgccgcg cgacggagcc ccgcgcgagg atgcccgtgt cggtcacact gccagtgtgt
     3181 ccctgatgcg atcgttcgcg tacgcgctct cagggcttgg gtatctcctc gcgacggagc
     3241 gcaacttccg catcgaggtg atggccgcgg cgctcgccgt gatcgccggg acgtggctcg
     3301 gcctcgagcg gctcgaatgg ctcgccctcg cgctcaccat cggtctcgtc ctcatcctcg
     3361 aggcgctgaa taccgcgatc gaggacgccg tgtcgctcgc gagccccggc ttcgacgagc
     3421 gggcacgcgc cgcgaaggat gtctccgccg ccgcggtgct ggtcgcgtcg ctcctggcga
     3481 tcgttgtcgg cctgctgctc ttcggtcccc gcataatccg cgtcggctga ggcatggccg
     3541 ttcgatcaaa ccggggagag agggcgatgg cggcgaccgg catccaccac gtgtacctgg
     3601 agacgcatag cttcgggaag accgcgaagt tctggcaggc cctggggttc gtgctcgacg
     3661 aagatcgcgg cacctcgggg ctcctccggc cacccgcggg cgggccgtac gtgtacgtcg
     3721 ccgaggtgcc cccgtcgcag accccgcgga cggagctcta cctcaacgcg gacgatgagg
     3781 cggtgcccgg ccgtcccgtc gaggtgaccg cgccctgggc cgacacgcac tgggggacgc
     3841 gcgagatggc gatacgcgac cccgacgggc ggacactgaa gctggagtcg cgcggcacgt
     3901 agccgcgacg gaaacgcgcc ggtccgcgac ctagactcgt cccatgccca tcgaccgcca
     3961 gacgatcgtt cacgtcgccg atctcgcgcg gatccagctc tccgacgagg agaccctgcg
     4021 gttcacggga cagctctcgg tcgtgctgga cgccgtcgcc cggctcgcag aggtcgacac
     4081 gagcgcggtc ctgccgagcg cgtcggtgct cccgctgcgg gacgtgcagc gcgaggatga
     4141 gtcgcggccg ggcctcacga acgagcaggc gctcgcgaac gctccgaagg gcgg
//
LOCUS       DASTZR010000299         3276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_617825, whole genome shotgun sequence.
ACCESSION   DASTZR010000299 DASTZR010000000
VERSION     DASTZR010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617825"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VFV20_06735"
     CDS             <1..674
                     /locus_tag="VFV20_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HEU5288080.1"
                     /translation="LNDLTRFVLVNAIYLKAKWQIPFEKSMTAAAPFHRLDGTDARVQ
                     LMRLPTPPSLPYARAAGYQAVSLPYIGGLSMVVLVPDAGSFASVEASLRDGGRLGQAV
                     DLPSRKAVKLALPRFTFRTAAMLKEPLEQLGMPIAFTDRADFSGIAPKERLLLQAVVH
                     EAFIAVDEEGTEAAAATAVIGGLTSAPSEIIELTVDRPFLFFIRDDETGVVLFMGRVV
                     DPSAS"
     gene            complement(717..1283)
                     /locus_tag="VFV20_06740"
     CDS             complement(717..1283)
                     /locus_tag="VFV20_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU5288081.1"
                     /translation="MRIFVAGASGVLGVRIVPLLVAGGHAVAAMTRTPAKAASLADLG
                     AQSVVCDVYDATALREAMARFHPDLVLDELTDLADDPRRIDAAANARIRVEGTRNVLA
                     AAGGARVISQSVAWPLRGEGQVAVEEHERLVRESGGVVIRYGQLYGPGTYHETAPESG
                     PRIHIDEAAHRTLEALGLAPGSVLTLVE"
     gene            1346..1873
                     /locus_tag="VFV20_06745"
     CDS             1346..1873
                     /locus_tag="VFV20_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013921029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEU5288082.1"
                     /translation="MKVACVLANGFEDSEFKQPYDALKEAGHEVTIVGLQAGQQVNGD
                     KGRETATVEKAFADVRPDQFDALLIPGGFSPDRLRAHPVAVEFVKAFFASRKPIMAIC
                     HGPQIFIPAGAYKDHRMTAWKTIQSDLKLLGANVVDEEVVVDRNLVTSRQPQDIPAFV
                     REGLKLLEKAPAAAR"
     gene            1902..2807
                     /locus_tag="VFV20_06750"
     CDS             1902..2807
                     /locus_tag="VFV20_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU5288083.1"
                     /translation="MRYRSLGRTDLRVSALGLGANQLGRIVDAAGAKAIIDRAQELGV
                     NFIDTAESYGDGASETLIGQALAGRRDRFVVATKTGAGSDPGRLTRERIAERLDASLR
                     RLATDHVDVYYLHMPDAATPLEESLRALEDARRAGKIRYPAISNHPAWQVAEAIALAE
                     RNAWTPVVASQMEYSLVERAVEREMVPACLHFGVSLVPYFPLAGGFLTGKYPRDVAPP
                     PNTRFGRAPQRSRFLSEANFDRLARWTAFAEERGRTVGELAVAWLLAHPVVPSVIAGA
                     TSAEQLGANAIAADWELSADEVSAL"
     gene            2891..>3276
                     /locus_tag="VFV20_06755"
     CDS             2891..>3276
                     /locus_tag="VFV20_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU5288084.1"
                     /translation="MKLRWATVVLPGVAIGLLDLWTDHDWDEVISEPVNTLIVPVLLL
                     SVAVTLAAAAFRRVDRLADDVREREAELARTAAVQEERERIAREMHDGLAQVLGYVNT
                     KAQAVEELLATGHTQEARTHLAELSSA"
BASE COUNT          475 a         1182 c         1152 g          467 t
ORIGIN      
        1 tgctcaacga cctgacgcgg ttcgtgctcg tcaacgccat ctacctcaag gcgaagtggc
       61 agatcccctt cgagaagtcg atgaccgcgg ccgcgccctt ccaccggctc gacggcaccg
      121 acgcgcgagt gcagctcatg cggcttccca cgccaccctc gctgccgtac gcgcgcgcgg
      181 ccgggtatca ggccgtgagc cttccctaca tcggcggact gtcgatggtc gtcctcgtgc
      241 ccgacgcggg atccttcgcc tcggtggagg cgagcctgcg cgacggcggc cgcctcggtc
      301 aggccgtcga cctgccttcg cgcaaggccg tgaagctggc gctgccgcgc ttcaccttcc
      361 gcaccgcggc gatgctcaag gagccgctcg agcagctcgg catgccgatc gcgttcaccg
      421 accgggcgga tttctctggg atcgcgccga aggagcggct cctgctccag gccgtggtcc
      481 acgaggcgtt catcgcggtc gacgaggaag ggacggaggc tgccgccgcc accgcggtga
      541 tcggcggtct cacgagcgcg ccgagcgaga tcatcgagct caccgtcgat cggcccttcc
      601 tgttcttcat ccgcgacgac gagaccggcg tggtcctctt catgggccgc gtcgtcgatc
      661 cgagcgcctc ctagccacgg agcgacaggg cccgagccag cgcatcactc gcgttttcac
      721 tcgaccagcg tgagcacgct ccccggcgcg aggccgagcg cctcgagggt gcggtgcgcg
      781 gcctcgtcga tgtggatccg cggtccggac tcgggcgcgg tctcgtgata ggtgcccggt
      841 ccgtagagct ggccgtagcg gatgacgacg ccgccgctct cgcggaccag acgctcgtgc
      901 tcctccaccg cgacctgccc ctcgccgcgc agcggccagg cgacgctctg cgagatcacg
      961 cgcgcgccgc cggccgcggc gaggacgttc cgcgtcccct cgacgcggat gcgcgcgttc
     1021 gccgcggcgt cgatgcgccg cgggtcgtcc gcgaggtcgg tgagctcgtc gagcacgagg
     1081 tccggatgga agcgcgccat cgcctcgcgc aaggcggtcg cgtcgtagac gtcgcagacg
     1141 acggactgcg cgcccaggtc cgccagcgaa gccgccttcg cgggcgtgcg cgtcatcgcc
     1201 gcgacggcgt gcccgcccgc cacgagcagc ggcacgatcc gcacgcccag cacaccgctc
     1261 gcaccggcga cgaagatccg catcgttcgc gctacaggct acgagcgcac aatcgagagc
     1321 gcacgacgac aggggaggtc aagacatgaa ggtcgcttgc gtgctcgcca acgggttcga
     1381 ggactccgag ttcaagcagc cgtacgacgc gctcaaggag gccggccacg aggtgacgat
     1441 cgtcggcctg caggccggtc agcaggtgaa cggcgacaag ggccgtgaga cggcgacggt
     1501 cgagaaggcg ttcgccgacg tgcgccccga ccagttcgac gcgctcctca tccccggcgg
     1561 gttctcgccg gatcgcctgc gcgcccatcc tgtcgccgtc gagttcgtca aggcgttctt
     1621 cgcgtcgcgg aagccgatca tggcgatctg ccacggcccg cagatcttca tccccgcggg
     1681 tgcgtacaag gaccaccgca tgaccgcgtg gaagacgatc caatcggacc tgaagctgct
     1741 cggcgcgaac gtcgtcgacg aggaggtcgt ggtcgaccgc aatctcgtga cgtcgcggca
     1801 gccgcaggac atcccggcgt tcgtccgcga agggctgaag ctgctcgaga aggcgcccgc
     1861 cgccgcgcgc taaaggcccc gcgccgccgt actctcgcgg gatgcgctat cgctcgctgg
     1921 gccgcaccga cctgcgcgtc tccgcgctgg gcctcggcgc gaatcagctc ggtcgcatcg
     1981 tcgacgccgc cggcgcgaag gcgatcatcg atcgcgcgca ggagctcggc gtgaacttca
     2041 tcgatacggc ggagagctat ggcgacggcg cgtcggagac cctgatcggt caggcactcg
     2101 ccggccgccg cgatcggttc gtcgtcgcga cgaagacggg cgccgggagc gacccaggcc
     2161 ggctgacgcg cgagcggatc gcggagcgtc tcgacgcgag cctgcggcgc ctcgcgaccg
     2221 atcacgtcga cgtgtattac ctccacatgc ccgacgccgc gacgccgctc gaggagtcgc
     2281 tgcgcgcgct ggaggacgcg cggcgggcgg gaaagatccg ctaccccgcg atctccaatc
     2341 acccggcgtg gcaggtggcc gaggcgatcg cgctggccga gcgcaacgcc tggacgccgg
     2401 tcgtcgcgtc gcagatggag tacagcctcg tcgagcgcgc ggtggagcgc gagatggtcc
     2461 cggcctgcct gcacttcggc gtctcgctcg tgccgtactt cccgctcgcg ggcgggttcc
     2521 tcaccggcaa gtacccgcgg gatgtcgcgc cgccgcccaa cacccggttc ggccgcgcac
     2581 cgcagcgctc gcgcttcctc agcgaagcga acttcgaccg gctcgcgcgc tggacggcgt
     2641 tcgccgagga gcgtggccga acggtggggg agctcgccgt cgcctggctc ctcgcgcatc
     2701 cggtcgtgcc gtccgtgatc gccggcgcca cgtcggcgga gcagctcggc gccaacgcga
     2761 tcgcggcgga ctgggagctg tcggccgacg aggtttccgc cctctagggc ccatcgaccc
     2821 ctgccggcgg gccattcggc cggatgagag ccggctgaga gcgcgtacgg tgcggcccga
     2881 ggctcgtcga atgaagctgc gctgggcaac ggtcgttctg ccgggagtcg cgatcggtct
     2941 gctcgacctg tggaccgatc acgactggga cgaggtcatc tccgaaccgg tcaacacgct
     3001 catcgtgccg gtcctgctgc tcagcgtggc ggtgacgctc gccgctgccg ccttccgccg
     3061 cgtcgaccgg ctcgccgacg acgtccgcga acgcgaggcg gagctcgcgc gcaccgcggc
     3121 ggtgcaggag gagcgcgagc gcatcgcgcg cgagatgcac gacgggctcg cgcaggtgct
     3181 cggctacgtg aacacgaagg cgcaggccgt cgaggagctg ctcgcgaccg gacacacgca
     3241 ggaggcgcgc acgcacctcg cggagctgtc gtccgc
//
LOCUS       DASTZR010000300         2470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1087543, whole genome shotgun sequence.
ACCESSION   DASTZR010000300 DASTZR010000000
VERSION     DASTZR010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087543"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..865
                     /locus_tag="VFV20_06760"
     CDS             164..865
                     /locus_tag="VFV20_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5288085.1"
                     /translation="MRPVTTDSRARILVVDDDEGVTSVVKRALERDGYDVATASSADE
                     ALRLVPDYRPDALVLDILMPGTDGVTLAKRLRAERPDTGILMVTAKDAAADQVTGLDA
                     GADDYLVKPFSLQVLEARLRAVLRRRQPSPEVIVVGDLELDMASRRARRGNREIDLTA
                     TEYRLLVQLMREAGHAIPKHELTQRVWGYDFEGNDNVLEVYIGYLRNKLEAQGEPRMI
                     HTLRGVGYEIRPVAA"
     gene            880..2268
                     /locus_tag="VFV20_06765"
     CDS             880..2268
                     /locus_tag="VFV20_06765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5288086.1"
                     /translation="MALAYGALLALVLVPALTIAYVLHAEAHDSDVDTALLDLTTTAQ
                     AQIDEQLRSGVPLRTVDISGLHQGIDEPYAVWFFVDDKAIASGGFVSDAAFQDVGPST
                     LTAGWHTNYTVNGRVRSYTVEIGVGRIVTAANLVAIDRANSDLRFAYVILGIFGIAFG
                     TAFASTAAGYLLRPVATLTETAAGIAESRDFKRRVPVKKEPEDELDVLALTFDEMLTN
                     LDDAYQQQQRFLGYVSHELRTPLTTIRGNAELLASSCDPSQSEQQEALERIARESARL
                     ARLVNELLMLARAEAAETFAPRAVDLDEVVMETFEQMRPMSDGRLRVRWIESARVNGE
                     RDRLKQLVLALVDNALRYTPPPGHVDVSLSDDGKDAVLRVEDEGIGIDDADLPHVFDR
                     FYRGAAARRSNATGSGLGLAIVRWIVQRHTGSILLDKREGGGTIVTVRIPLATPSLKQ
                     PQPAAHAEPAPA"
     gene            2333..>2470
                     /locus_tag="VFV20_06770"
     CDS             2333..>2470
                     /locus_tag="VFV20_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011374773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEU5288087.1"
                     /translation="MSQRVAIYPGSFDPPTNGHIDIVERSSRLFDRVVVAIGRNLAKK
                     TV"
BASE COUNT          400 a          870 c          823 g          377 t
ORIGIN      
        1 gaccctgcca tacgccttcc ttccgtttgt tgccttcctg tgtcccctca gcggagcctc
       61 aggtgatgcg actttcttcc tgagcacacc ctgagggaag gacctaagga ccctctcatc
      121 tggtccgact ggcagattcg cctgaggggg aacggcaccg accatgcgac cagtgacgac
      181 tgactctcgg gcccggatcc tggtcgtcga cgacgacgaa ggcgtgacgt cggtggtgaa
      241 gcgcgcgctc gagcgcgacg ggtatgacgt ggcgaccgcg agcagcgccg acgaggcgct
      301 gcgcctggtc cccgattacc ggcccgacgc gctggtcctc gacatcctca tgcccggcac
      361 cgacggtgtg acgctggcca agcgccttcg cgcggagcgc ccggacaccg ggatcctcat
      421 ggtcaccgcg aaggacgccg ccgcggacca ggtcaccggc ctcgacgcgg gggcggacga
      481 ctacctggtg aagcccttca gcctgcaggt gctggaggcg cgtctgcgcg cggtgctgcg
      541 gcggcgccaa ccctctcctg aagtgatcgt cgtaggcgat ctcgagctgg acatggcgag
      601 ccgccgggcg cgccgcggga atcgtgagat cgacctcacc gcgaccgagt accggctgct
      661 cgtgcagctg atgcgcgagg ccggccacgc gatcccgaag cacgagctga cgcagcgggt
      721 ctggggctac gacttcgaag gcaacgacaa cgtcctcgag gtctacatcg gctacctgcg
      781 caacaagctc gaggcccagg gcgagccgcg catgatccac acccttcgcg gtgtggggta
      841 cgagatccga ccggtcgcgg catgaccctc cggcgccggt tggcgctggc ctacggagcc
      901 ctcctcgcgc tcgtcctcgt tcctgccctc accatcgcgt acgtcctcca tgccgaggca
      961 cacgactccg acgtcgatac cgcgctgctc gacctcacga cgacggcgca ggcgcagatc
     1021 gacgagcagc tgcggtccgg agtgccgctg cgcactgtcg acatcagcgg gctccatcag
     1081 ggcatcgacg agccgtacgc ggtgtggttc ttcgtcgacg acaaggcgat cgcctccggc
     1141 ggcttcgtct ccgacgccgc gttccaggac gtcggaccat cgaccctcac cgccggctgg
     1201 cacaccaact acacggtgaa cggccgggtg cgcagctaca ccgtcgagat cggcgtcggc
     1261 cgcatcgtca cggcggcgaa tctggtcgcg atcgatcgag cgaacagcga cctgcgcttc
     1321 gcctacgtga tcctcggtat cttcggcatc gcgttcggca cggcgttcgc gtccaccgcg
     1381 gcgggatacc tgctgcgccc ggtcgcgacg ctcaccgaga cggcggccgg catcgccgag
     1441 tcgcgcgact tcaagcggcg cgtgccggtc aagaaggagc cggaggacga gctcgacgtg
     1501 ctcgccctca cgttcgacga gatgctcacc aatctcgacg acgcgtacca gcagcagcag
     1561 cgtttcctcg ggtacgtgtc acacgagctg cgcacgccgc tcacgacgat ccgcgggaat
     1621 gccgagctcc ttgcttcgag ctgcgatccg agccagtccg agcagcagga ggcgctcgag
     1681 cgcatcgcac gcgaatcggc acggctggcg cggctcgtca acgagctgct catgctcgcg
     1741 cgcgcggagg cggccgagac gttcgcgcca cgcgcggtcg acctcgacga ggtcgtcatg
     1801 gagacattcg agcagatgcg gcccatgtcc gacggccggc tgcgcgtgcg ctggatcgag
     1861 agcgcgcgcg tgaacggcga gcgcgatcgg ctgaagcagc tcgtgctcgc gctcgtcgac
     1921 aacgcgcttc ggtacacgcc accgccgggc cacgtcgacg tgtcgctgtc ggacgacggg
     1981 aaggacgcgg tgctgcgggt cgaggacgag ggcatcggca tcgacgatgc cgacctgccg
     2041 cacgtgttcg accgcttcta ccgcggtgcg gccgcgcgtc gttcgaacgc gacgggaagc
     2101 ggcctcggac tggccatcgt gcgctggatc gtgcagcgcc acaccgggtc gatccttctc
     2161 gacaagcgcg agggcggcgg caccatcgtg acggttcgca tcccgctcgc gaccccttct
     2221 ctgaagcagc cgcagcccgc cgcgcacgcg gagcctgcgc ccgcctaggc tcgtcgcgtc
     2281 gcggcattag gattcagcca ggcgtgcact accagaagga ggcgcgaccc gagtgagcca
     2341 gcgcgtcgcc atctaccccg gatccttcga tccaccgacg aacggacaca tcgacatcgt
     2401 cgaacgctcg tcgcgcctct tcgaccgcgt cgtggtggcg atcggccgca atctcgcgaa
     2461 gaagacggtg
//
LOCUS       DASTZR010000301         4052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_461270, whole genome shotgun sequence.
ACCESSION   DASTZR010000301 DASTZR010000000
VERSION     DASTZR010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461270"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..172)
                     /locus_tag="VFV20_06775"
                     /pseudo
     CDS             complement(1..172)
                     /locus_tag="VFV20_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006567599.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
     gene            162..605
                     /locus_tag="VFV20_06780"
                     /pseudo
     CDS             162..605
                     /locus_tag="VFV20_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874021.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
     gene            605..1021
                     /locus_tag="VFV20_06785"
     CDS             605..1021
                     /locus_tag="VFV20_06785"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEU5288088.1"
                     /translation="MPLHLEVITPERRVYEDDVDMVIAPGADGYLGILPHHAPLLTSL
                     GPGEFRVKKGGTEEVLAVFGGFMDVRADHVVVLTDAAEPVEDIDLQRAQEARERAQKD
                     LAAGLPSAADEQRARAALQRALIRIRVSERRRRRNL"
     gene            1062..2246
                     /locus_tag="VFV20_06790"
     CDS             1062..2246
                     /locus_tag="VFV20_06790"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HEU5288089.1"
                     /translation="MDGIRSERAPEKADWWDRVKRFAILMTAAVVLASCGSARVAPPA
                     PTTSPEAPTAAPTAAPTTPTASPEPTATPDPRQYAKLRVFVASENTDQVWVMEGAPGG
                     EYALLTKIPVGRLPHQMAVAPNGKFVAVNNRMSNTTSVIDPFTMKEIQRIPVGKQPHG
                     ITFSVDSKTLYVAHERDPYILAIDTATWKTTAFMVGVPQHVLTISAAHPNEIWFTVTN
                     SSETDVLRVIDVTTKEITKVKVGDVHDAYFTPDGSEIWSSSSGFLDKPSDRMVIYDPA
                     TRKVKEEIRLTGHYPFHTLKPFQDGMYALREPGLIVLSDHTFPGLMWVDWRERRVVGE
                     TKLGQQPFHSTYDPEGNRLLVTTNVDGMVNVIDLKTRQVVQKVPVSKPHGIVSVGIQA
                     GQ"
     gene            2372..2839
                     /locus_tag="VFV20_06795"
     CDS             2372..2839
                     /locus_tag="VFV20_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEU5288090.1"
                     /translation="MRALALVFNAFPVRWQRRFMTAAHQRFLVGVVGLGVDAEGRVLL
                     ARHRFGAPEWRFLGGFLAAKERPEDALAREIREETGLAIEVGPVLEATTGFRWQKVEL
                     VFPYRVVGGTQVLSDEVAELRAFPPGDLPEVRADQRGLVERHSAAAADWARSR"
     gene            2842..3216
                     /locus_tag="VFV20_06800"
     CDS             2842..3216
                     /locus_tag="VFV20_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010225017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5288091.1"
                     /translation="MRIDHVVLTVRDIDRTVDFYTRALGMRAIAFGDGRRGLAFGDQK
                     LNLHLAGREFEPKARVPTPGSVDLCLVTDAPLDDVRERLAALGIAIEVGPVAKVGARA
                     PLRSIYVRDPDGNLIEIANETD"
     gene            complement(3235..3897)
                     /locus_tag="VFV20_06805"
     CDS             complement(3235..3897)
                     /locus_tag="VFV20_06805"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEU5288092.1"
                     /translation="MTEPVIYVWGQGEYWLADVPAFPGCIASGKTRDAAIANARRAFR
                     AYRELLDARGVSVDHWKDLDPDSFAVRDMPESGLAPEDERPLEEHEIRDFLHLYEAQR
                     AALIALVDRLPQSEMERRPEPASWSVRNTLEHIMTGTLTILSRLERWPDDGFASLQAT
                     HRIAFQRITVMEPHDTAGTKTVMGRPQTVRRVMRRLLEHDYEHYRQIEEIIAALGRRP
                     DA"
BASE COUNT          626 a         1377 c         1431 g          618 t
ORIGIN      
        1 cgtcgaggcc gaggatggcg atgatgtcct gcaggtcctt gtagcgctgc agcacctgct
       61 tgacgcggcg ggcgaccgag tagtgctcgt cgccgatgat gcgcgggtcg aggatgcgcg
      121 aggtcgaggc gagcggatcg acggccgggt agatcccctt ctcgacgatc gcgctggagc
      181 gctcgatcgc cgagcagggc ctctacccgg cggtggaccc gctgtcctcg acgtcgcgga
      241 tgctcgaccc gcgcgtcgtc ggcgacgagc actacgcggt cgcccgcgag gtccagcgcg
      301 tgctgcagcg ctacaaggac ctgcaggaca tcatcgccat cctcggcgtc gacgagctgt
      361 ccgacgacga caaggtcctc gtggcacgcg cgcgcaagat ccagcgcttc ctcgcgcagc
      421 cgctgaacgt ggccaagcag ttcaccggcc tcgagggcgt ctacgtgaag gtcgccgaca
      481 ccgtccgcag cttccaggag gtcctcgagg gcaagcacga cgatctgccc gagcaggcct
      541 tcttcaatgt cggcacgatc gagcaggctc gcgagaaggg cgagcggctc gcgaagggcg
      601 cctgatgccg ctccatctcg aggtcatcac gcctgaacgt cgtgtgtacg aggacgacgt
      661 ggacatggtc atcgcgccgg gcgccgacgg ctacctgggg atcctgccgc accacgcgcc
      721 gctcctgacg tccctcggac ccggcgaatt tcgcgtgaag aaaggcggca ccgaggaggt
      781 gctcgcggtc ttcggcggat tcatggacgt tcgggccgat cacgtcgtcg tcctgacgga
      841 cgcggccgag ccggtggagg acatcgacct ccagcgggcc caggaagcgc gggaacgcgc
      901 gcagaaggac cttgcggcgg ggctgccgtc cgccgccgac gagcagcggg ctcgcgcggc
      961 actccagcgt gctctcatcc gtatccgtgt cagtgagcgg agacgccgcc ggaatctttg
     1021 accggcccgt agcgttcgcg cccgcacacc gataggctga cttggacggc atccgttcag
     1081 agcgggcgcc ggagaaagcg gactggtggg accgggtgaa gcgcttcgcg atcctcatga
     1141 cggccgccgt cgtccttgcg tcctgcggga gcgcacgcgt cgcgccgccc gcgccgacga
     1201 cgtcgcccga agcccctacg gccgcgccga ccgccgcgcc taccacgccg accgcgtcac
     1261 cggaaccgac agcgacgccc gatccacgtc agtacgcgaa gctccgcgtg ttcgtcgcat
     1321 cggagaacac ggaccaggtc tgggtgatgg agggcgcgcc gggcggcgag tacgccctgc
     1381 tcacgaagat cccggtgggc cgcctgccgc atcagatggc cgtcgcgccg aacggcaagt
     1441 tcgtcgcggt gaacaaccgc atgtccaaca cgacctccgt gatcgacccg ttcacgatga
     1501 aggagatcca gcgcatcccg gtggggaagc agccgcacgg gatcacgttc tccgtggaca
     1561 gcaagacgct ctacgtcgcg cacgagcgcg atccgtacat cctcgcgatc gacacggcga
     1621 cgtggaagac caccgccttc atggtgggcg tcccacagca cgtgctcacg atcagcgcgg
     1681 cccatccgaa cgagatctgg ttcaccgtga cgaactccag tgagacggat gtgctccgcg
     1741 tgatcgacgt gaccacgaag gagatcacca aggtcaaggt cggcgacgtg cacgacgcct
     1801 acttcacgcc ggacggcagc gagatctggt cgagcagcag tggcttcctc gacaagccgt
     1861 ccgatcgcat ggtcatctac gatcccgcga cgcgaaaggt gaaggaggag atccgcctca
     1921 ccggccacta cccgttccac acgctgaagc cgttccagga cggcatgtac gccctgcgcg
     1981 agccggggct catcgtgttg tccgatcaca ccttccccgg tctcatgtgg gtcgactggc
     2041 gcgagcggcg cgtcgtgggc gagacgaagc tcggccagca gcctttccac tcgacgtacg
     2101 accccgaggg gaaccgcctg ctggtcacca cgaacgtcga cggcatggtg aacgtgatcg
     2161 acctgaagac gcgccaggtc gtgcagaagg tcccggtgtc gaagccgcac ggcatcgtca
     2221 gcgtcggcat ccaggcgggg caatgatcgc ggtgcttcgc cgctgactcg ggcccgcacc
     2281 cgcggggagc gcggcgcgct gccgcggagt actgccccta ggaccggcat cgccgcgatg
     2341 ccgtgcgctc tctagagttg cggctgtgct catgcgcgcg ctggcgctcg tcttcaacgc
     2401 gttcccggtg agatggcagc gacggttcat gacggcggcg catcagcgtt tcctcgtggg
     2461 ggtcgtcggg ctcggggtcg acgcggaggg acgcgtgctc ctcgcgcgac atcgtttcgg
     2521 cgcgccggag tggcgcttcc tcggcggatt cctcgccgcg aaggaacgtc ccgaggacgc
     2581 gctcgcgcgc gagatccgcg aggagacggg cctcgccatc gaggtcggtc cggtgctcga
     2641 ggcgaccacc ggcttccgct ggcagaaggt cgagctcgtc tttccctacc gggtcgtcgg
     2701 cggtacgcag gtgctgtcgg acgaggtcgc cgagctgcgg gcgttcccgc cgggtgatct
     2761 cccggaggtg cgcgcggacc agcgcgggct cgtcgagcgc catagcgcgg cggcggcgga
     2821 ctgggcgcgc tcgcggtgag cctgcgcatc gaccacgtcg tgctcaccgt gcgcgacatc
     2881 gatcgcaccg tcgacttcta cacgcgggcc ctcggcatgc gcgcgatcgc gttcggcgac
     2941 ggcaggcgcg gcctcgcgtt cggtgaccag aagctgaacc tccatctggc cggtcgcgag
     3001 ttcgagccga aggcgcgcgt gcccacaccc gggtcggtcg atctgtgtct cgtcaccgac
     3061 gcgccgctcg acgacgtgcg cgagcgcctg gccgcgctcg gcatcgcgat cgaggtgggg
     3121 ccggtggcga aggtcggcgc gcgcgcgccg ctccgctcga tctacgtccg cgacccggac
     3181 ggcaacctca tcgagatcgc gaacgagacg gactgatccg gggcggggcg cggctcaggc
     3241 gtcgggtcgt cggccgaggg cggcgatgat ctcctcgatc tggcggtagt gctcgtagtc
     3301 gtgctcgagc agccgtcgca tgacgcggcg gaccgtctgc gggcggccca tcacggtctt
     3361 cgtccccgcc gtgtcgtgcg gctccatgac ggtgatccgc tggaaggcga tgcggtgggt
     3421 cgcctgcagg gacgcgaagc catcgtcggg ccagcgttcg agcctggaga ggatcgtcag
     3481 cgtcccggtc atgatgtgct cgagcgtgtt ccgcacgctc cacgacgcgg gctcgggacg
     3541 gcgctccatc tccgattgcg gaaggcggtc gacgagggcg atgagcgccg cgcgctgcgc
     3601 ctcgtagaga tggaggaagt cgcggatctc gtgctcctcg agcggtcgct cgtcctccgg
     3661 tgcgaggccg ctttcgggca tgtcgcggac ggcgaagctg tcgggatcga gatccttcca
     3721 gtgatcgaca ctgacgccgc gggcgtccag cagctcgcga tacgcgcgga acgcgcgccg
     3781 ggcgttcgcg atcgcggcgt cgcgtgtctt gcccgacgcg atgcaccctg ggaacgccgg
     3841 gacgtcggcg agccagtact cgccctgtcc ccacacgtag atgaccggct cggtcatgcg
     3901 cgcaccagca ccttgcgccc gcagtcgcgg cggacggacc gctccgtgcc gtcgataccg
     3961 aggcggagcg tgtcgtcggc gatcgcggcg agccgcacga cgacgcccgg cttcacgccg
     4021 acgctgtcga ggtactggag gaagccgggc tc
//
LOCUS       DASTZR010000302         2268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_561344, whole genome shotgun sequence.
ACCESSION   DASTZR010000302 DASTZR010000000
VERSION     DASTZR010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561344"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1258
                     /locus_tag="VFV20_06810"
     CDS             <1..1258
                     /locus_tag="VFV20_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5288093.1"
                     /translation="RILIVDDIADTRENLAKLIGFEPDMEVAGAAAGGQEAVNLAKKE
                     RPHVILMDINMPDMDGITATEIISNTVPESPIIMMSVQGEQDYLRRSMLAGAREFLVK
                     PFSADELINSIRHVHELEKVRRARYAPVQQQGGGGQGTAAPVPRETGKIITFFSPKGG
                     VGRTTIATNLAVALHQMTGKPVVLVDGSLPFGDIAVILNMSPKAKTLADLIGSFEQAD
                     ADVVESILVQHSTGIKVMLAPPTPESTELITGANMKHILEILRERYAYVVVDTWPSFQ
                     EQVITMLDVADVILTLMTLEITSLKNVRVFMEIAEKLGYDENKVQLVANRNDSSGGIK
                     ASDVEASLGRKIPHTIVSDGRTLVLAVNRGVPFVTSNRDSQVSKDIFALAAKLSAGAN
                     EAAAAPAGTGKQAKQTSGGLKLFGAR"
     gene            1411..>2268
                     /locus_tag="VFV20_06815"
     CDS             1411..>2268
                     /locus_tag="VFV20_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEU5288094.1"
                     /translation="MSLLRRIEGQRPTQQPAAPAAPGAPTPPPGAPPKAPAEGPLSQT
                     VAPARDSNRDLRFRLQTRIINNLDPRLDLSDAKAVRANIDEMFNKFLDEEGTVITRVE
                     RQKMLEAILDEILGFGPIQPLLNDDTITEVMVNGPFRVYVERKGKLTLTDVTFQNDEH
                     VMRIIERIIAPIGRRVDESKPYEDARLPDGSRVNIIIPPLALNGPVITIRKFPKYRIT
                     VEDYIKFGTATPEMMEFLRACVEARLNVFISGGTGSGKTTLLNILSSFIPNNERIVTI
                     EDAAELQLRQ"
BASE COUNT          439 a          806 c          696 g          327 t
ORIGIN      
        1 ccggatcctc atcgtcgacg acatcgccga cacgcgcgag aacctcgcca agctcatcgg
       61 gttcgagccc gacatggagg tcgccggcgc ggccgccggc gggcaggagg ccgtgaacct
      121 cgcgaagaag gaacggcctc acgtgatcct catggacatc aacatgcccg acatggacgg
      181 gatcacggcg accgagatca tctccaacac cgtccccgag tcgccgatca tcatgatgtc
      241 ggtccagggc gagcaggact atctgcgccg ctcgatgctg gccggagccc gcgagttcct
      301 ggtgaagccc ttctccgcgg acgagctcat caactcgatc cggcacgtcc acgagctcga
      361 gaaggtgcgc cgtgcgcgct acgcccccgt gcagcagcag ggcggcggcg gtcagggtac
      421 ggccgctccg gtgccgcgcg agaccgggaa gatcatcacg ttcttctcac caaagggcgg
      481 cgtcggccgc acgaccatcg cgaccaacct cgccgtcgcg ctgcaccaga tgaccggcaa
      541 gccggtcgtg ctcgtggacg gcagcctgcc gttcggcgac atcgccgtga tcctcaacat
      601 gagtcccaag gccaaaacgc tcgccgatct catcggctcg ttcgagcagg cggacgcgga
      661 cgtcgtcgag tcgatcctgg tgcagcactc gaccggcatc aaggtcatgc tcgcgccgcc
      721 gacgccggaa tccaccgagc tcatcaccgg cgccaacatg aagcacatcc tcgagatcct
      781 gcgcgagcgc tacgcatacg tcgtcgtcga tacgtggccg tcgttccagg agcaggtcat
      841 caccatgctc gacgtcgccg acgtgatcct cacgctgatg acgctcgaga tcacgagcct
      901 caagaacgtg cgcgtcttca tggagatcgc cgagaagctc gggtacgacg agaacaaggt
      961 ccagctcgtc gcgaaccgta acgactcctc cggcggcatc aaggcgagcg acgtcgaggc
     1021 gagcctcggg cgcaagatcc cgcacaccat cgtctcggat ggccgcacct tggtgctggc
     1081 cgtgaaccgc ggcgtgccgt tcgtcacgag caaccgtgac agccaggtgt ccaaggacat
     1141 cttcgccctc gccgccaagc tctccgccgg ggcgaacgaa gccgcggcgg cgccggccgg
     1201 cacgggcaag caggccaagc agacgagcgg cggcctcaag ctcttcggcg cgcgctagaa
     1261 cccccgacag cgccgtccgg acaggacgat tagcgacgcg gttgacccct tcggggggcg
     1321 ccaagtgtcg cattctcgga cgtacactcc tgcggcgagg gagcagacgg ccaataacgg
     1381 ccgcctcaga tcgaggttgg taaggagcac atgagtctcc tcagacgcat cgaaggccag
     1441 cgtccgacgc agcagcctgc ggcacccgcc gcgcccggcg ccccgacacc gccccccggc
     1501 gcgccaccca aggcgcccgc cgaggggccg ctcagccaga cggtcgcgcc cgcgcgcgac
     1561 tccaaccgcg acctccgctt ccgcctgcag acgcggatca tcaacaacct cgacccgcgc
     1621 ctcgacctgt cggacgcgaa ggccgtgcgg gcgaacatcg acgagatgtt caacaagttc
     1681 ctcgacgaag agggcacggt catcacgcgc gtcgagcgcc agaagatgct cgaggcgatc
     1741 ctcgacgaga tcctcggatt cggtccgatc cagccgctcc tcaacgacga caccatcacc
     1801 gaggtcatgg tgaacggtcc gttccgggtc tacgtggagc gcaagggcaa gctgacgctg
     1861 acggacgtga ccttccagaa cgacgagcac gtcatgcgca tcatcgagcg gatcatcgcc
     1921 ccgatcggcc ggcgcgtcga cgaatccaag ccgtacgagg acgcgcgcct tccggacggc
     1981 tcgcgcgtga acatcatcat cccgccgctc gccctgaacg gcccggtcat cacgatccgg
     2041 aagttcccga agtaccgcat caccgtcgag gactacatca agttcggcac cgcgacgccc
     2101 gagatgatgg agttccttcg cgcgtgcgtc gaagcccggc tcaacgtgtt catctccggt
     2161 ggcaccggct ccggcaagac gacgctgctg aacattttgt cgtccttcat cccgaacaac
     2221 gagcgcatcg ttaccatcga ggatgccgcc gagctgcagc ttcgtcag
//
LOCUS       DASTZR010000303         2364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_513634, whole genome shotgun sequence.
ACCESSION   DASTZR010000303 DASTZR010000000
VERSION     DASTZR010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513634"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /gene="mltG"
                     /locus_tag="VFV20_06820"
     CDS             <1..1040
                     /gene="mltG"
                     /locus_tag="VFV20_06820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU5288095.1"
                     /translation="VRPLAQDAFVAYVADHPTLLKQQVVRSLVATRVSGEVDLAKDIR
                     GEARPFVIERGETAGEIARRLQSEGLVRDRLAFEFVLYENGKEDALQSGTYQVSPALT
                     PRDIARLFEKAPGEQVVLRVIEGWRLTEIAAAVAKAFPKITREAFIAAAVVGDRKSPA
                     LAGMDPKAPLEGFLYPDTYFMRPETTATQIVDTLLSVFEDRAGATLRAAATQRKMSVY
                     DLVKLASLVEREARDRSESATIAGVYANRLAIGMKLDADPTIQYALAEWRELSLDDLK
                     LDSPYNTYLKAGLPPTPICSPGQSALEGAAKPAQHDYLYFVAKGDGTGQHAFAKTLEE
                     QEANRVKYGNR"
     gene            1040..1831
                     /gene="aroE"
                     /locus_tag="VFV20_06825"
     CDS             1040..1831
                     /gene="aroE"
                     /locus_tag="VFV20_06825"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEU5288096.1"
                     /translation="MSRVVLLGHPVAHSLSPLMHNAAFAALGLRHRYEAVDVPPDALS
                     RTVDGLRREDALGANVTIPHKEAALRLVDETSDDVRAIGALNTIVRRGSLLIGHNTDA
                     YGFEEAIAGIGAGDALVLGAGGAARACVLVLLRQRRRVLVANRSKTRAEELARTLSVD
                     GRHAEVTTWPYGRELAAPIVVNATPLGMHGEDPLRGTPLPRCVVDIVPMTEETPLVRR
                     AREAQHVVVVDGITMLLHQAARSFELWTGLAAPLEVMRAALPRPA"
     gene            1828..>2364
                     /locus_tag="VFV20_06830"
     CDS             1828..>2364
                     /locus_tag="VFV20_06830"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU5288097.1"
                     /translation="MRLLTAGESHGPGLVTLIDGVPAALPLGPADIDPDLHRRQLGYG
                     RGARQRIERDTVRITAGVRQGRTTGAPIVLQIANRDAVNWERVMSVEPVPDAPPAVTR
                     LRPGHADLAGALKFAHGDVRDVIERSSARETAARVAGGALAKAFLRAVGVEVRSHVTQ
                     IGSVLAPERDEPLALEDFE"
BASE COUNT          348 a          827 c          863 g          326 t
ORIGIN      
        1 tggtccgccc gctggcgcag gacgcgttcg tggcctacgt ggcggatcac cccacgctgc
       61 tcaagcagca ggtggtgcgg tcgctcgtcg cgacgcgcgt gagcggcgag gtggatctcg
      121 cgaaggacat ccgcggcgag gcgcgaccgt tcgtcatcga acgtggcgag acggcgggcg
      181 agatcgcgcg ccggctgcag agcgagggcc tggtccgcga tcgtctggcg ttcgagttcg
      241 tgctgtacga gaacggcaag gaggacgccc tgcagtcggg cacgtaccag gtgtcgcccg
      301 cgctcacgcc gcgcgacatc gcgcgcctct tcgagaaagc acccggcgag caggtggtcc
      361 tccgcgtcat cgaaggctgg cgtctcaccg agatcgccgc ggccgtggca aaggcattcc
      421 ccaagatcac cagggaggcg ttcatcgcgg cggccgtggt cggcgaccgc aagagcccgg
      481 cgctcgcggg gatggacccg aaggcgccgc ttgagggctt cctgtacccc gacacgtatt
      541 tcatgcggcc cgagaccacg gcgacccaga tcgtcgacac gctgctttcc gtattcgagg
      601 accgcgcggg cgcgacgctg cgcgcggcgg ccacgcagcg gaagatgtcc gtctacgacc
      661 tggtgaagct cgcgtcgctc gtcgaacgcg aggcgcgcga tcgctcggag agcgccacca
      721 tcgccggggt ctacgcgaac cgcctcgcga tcgggatgaa gctcgatgcc gatcccacga
      781 tccagtacgc actggccgag tggcgcgagc tgagcctcga cgacctcaag ctggattcgc
      841 cgtacaacac gtacctcaag gccggcctgc cgccgacgcc gatctgcagt cccggacagt
      901 ccgcgctcga gggcgcggcg aagcccgcgc agcacgacta cctctacttc gtcgcgaagg
      961 gcgatggcac gggccagcac gcgttcgcga agacgctcga ggaacaggaa gcgaaccggg
     1021 tcaagtacgg gaatcggtag tgagtcgcgt cgtcctgctc gggcatccgg tcgcgcactc
     1081 gctctcgccg ctcatgcaca acgcggcctt cgcggcgctc ggcctccggc accgctatga
     1141 ggcggtcgat gtgccgcccg acgcgctgtc gcggacggtg gacgggctcc gtcgcgagga
     1201 cgcgctcggc gcgaacgtga cgatcccgca caaggaggcc gcgctgcgcc tcgtcgacga
     1261 gacgagcgac gacgtgcggg cgatcggcgc gctcaacacg atcgtgcggc gcgggtcgct
     1321 gctcatcggc cacaacaccg acgcgtatgg cttcgaggag gcgatcgcgg gtatcggagc
     1381 cggcgacgcg ctcgtgctcg gcgcgggcgg ggccgcgcgt gcgtgcgtgc tcgtgctgct
     1441 tcgccagcgg cggcgggtgc tcgtggccaa ccgatccaag acgcgggccg aggagctcgc
     1501 acggacgctg tcggtcgacg gtcgccacgc cgaggtcacg acctggccgt acggacgcga
     1561 gctcgccgcg cccatcgtgg tgaacgcgac gccgctcggg atgcacggcg aggatccgct
     1621 gcggggcaca cccctaccac gctgcgtcgt ggacatcgtg cccatgacgg aggagacgcc
     1681 gctcgtcaga cgcgcgcgtg aggctcaaca tgtcgttgtc gtggatggca tcaccatgct
     1741 cctgcatcag gcggcgcgct ccttcgagct gtggacgggc cttgcggcac cactcgaggt
     1801 gatgcgggcg gcgctgccac gtccggcatg aggctcctca ccgcgggcga gtcgcacggt
     1861 ccgggcctgg tgacgctcat cgacggcgtg ccggcggcgc tgccgctcgg tcccgcggac
     1921 atcgaccccg acctgcaccg ccgccagctc ggctacggcc gtggcgcgcg gcagcgcatc
     1981 gagcgcgaca ccgtgcgcat caccgccggc gtgcgccagg ggcggaccac cggtgcgccg
     2041 atcgtgctgc agatcgccaa tcgcgatgcg gtgaactggg agcgggtgat gagcgtcgag
     2101 cccgtgcccg acgcgccgcc ggcggtcacg cgcctgcggc ccgggcatgc cgacctcgcc
     2161 ggcgccctga agttcgcgca cggcgacgtg cgtgacgtca tcgagcgctc gagcgcgcgc
     2221 gagaccgccg cgcgcgtcgc cggcggtgcg ctggcgaagg cgttcctgcg cgcggtcggc
     2281 gtcgaggtcc gctcgcacgt cacgcagatc ggctccgtgc tcgccccgga gcgcgacgag
     2341 ccgctggcgc tggaggactt cgag
//
LOCUS       DASTZR010000304         3643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_695956, whole genome shotgun sequence.
ACCESSION   DASTZR010000304 DASTZR010000000
VERSION     DASTZR010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695956"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..1378
                     /locus_tag="VFV20_06835"
     CDS             254..1378
                     /locus_tag="VFV20_06835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEU5288098.1"
                     /translation="MRAQDGIRRELIALSREIHGDPELAYKEERGVARIAALLRAHGH
                     TVEMGIGGLATAFRARVGPSGKSVAMLAEYDALAGVGHGCGHNLIAMTNVGAFLLAAA
                     DPERLEVPVELIGTPAEEDGGGKIDLLRAGVFETVGYALSTHPAAQAAWVVSATGLGI
                     VGKRVIYRGRSSHAGSSPERGRNALSGVIRLFTGIDGWRQQLPTDSRVHGIIDFGGDA
                     VNVVPSRAEARFGLRARDLDTLREMEVAFTNVAQGAALETSTTVEISDYLPLYAPVAA
                     NATLGDAVAAELRARGVEPERGTLVTASTDLGNVSQRVPTDWIRFPVSEHAIPGHSDE
                     MRDACVTDRAHANAIVTMEALGAAARRVATDGRLRASIAA"
     gene            1375..2490
                     /locus_tag="VFV20_06840"
     CDS             1375..2490
                     /locus_tag="VFV20_06840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU5288099.1"
                     /translation="MSASLRDDLIVLSRAIHGTPELAYQERQAVANITALLRRHGHDV
                     EAKLGGVETAFRAHLGPKGPSVALLAEYDALPEIGHACGHNLIAMSNVGAFLLAAQRE
                     RDLQVGIELIGTPAEESGGGKIDLIDAGVFRESVAVLSSHPGGNEWELGGSSLGIVGK
                     LVRFHGVASHAAYSPHKGRNALTAAIRLFLAVDGWRQHLPADTRVHGIITKGGAASNI
                     IPDLAECDFGLRAASLDYLEEMVATFRQMAEGAAMQTGTTVEVDDKMRLYAPVRPNAK
                     IGDLLVAELAKRRLEVVRGRLTLASTDFGNVSQVAPADYIGFPVSTEPVPGHSHQMRE
                     SSASDYAHDAAMTTAEVLATVAVRIATDPAVRASLRA"
     gene            2487..3443
                     /locus_tag="VFV20_06845"
     CDS             2487..3443
                     /locus_tag="VFV20_06845"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEU5288100.1"
                     /translation="MNSNDTVHPVPLTDIAAARWLDVDFPGLEFGIAEYAEGPTGCTV
                     FHFPELASMHIDVRGGSPGINGEHLTLTNAICFAGGSLYGLEASTGVAAELFARRGYS
                     TKWMDIALVSGAIIFDYGRRENTVYPDKELGRAAVRAFRPGRFPLGPHGAGISATAGH
                     GRTAELSGQGGAWRRVGATRVAVFTVVNAYGAIIDRTGSVLRGNRDPHTGTRSRAERM
                     IETTGAYDPPPGRIRGVSQNTNLTLVVTDQKLDPLQLRSVARQTHSSMARAIQPFHTR
                     WDGDILFAVSTQRVANAALDEVSLAVIASDLAWDAVLASFDP"
BASE COUNT          530 a         1309 c         1264 g          540 t
ORIGIN      
        1 cgcgagtcct gctggctctg cctcgtctcc ggcgagccct gcttcgcctc tgcggtgctc
       61 tgcttcgtgt ctggcgagcc ctgtttcgtg tctgacgcgc tctgttctgg gttttccaac
      121 ctggtggtcg ctccttgggg tgatatgccg cgatagggat gctgtgggaa acaccggggg
      181 cgctcgatgg agaatgcgcc ggcacccggc gagggaagat ggggaaagtc tctacacggc
      241 agtatacccc ctggtgaggg cccaggacgg catccgacgc gagctcatcg cgctctcgcg
      301 tgagatccac ggcgaccccg agctcgcgta caaggaggaa cgtggggtcg cccgcatcgc
      361 cgcgctcctg cgcgcgcatg gccacacggt cgagatgggg atcgggggcc tggcgaccgc
      421 gttccgcgcg cgcgtggggc cgagtggcaa gagcgtcgca atgctcgcgg agtacgacgc
      481 gctcgccggc gtcgggcacg gctgcgggca caacctcatc gccatgacga acgtcggcgc
      541 cttcctgctt gctgccgccg accccgagcg gctcgaggtg ccggtggagc tcatcggcac
      601 acccgcggag gaggacggcg gcgggaagat cgacctgctg cgcgcggggg tgttcgagac
      661 cgtcggctac gcgctctcga cgcatccggc ggcgcaggcc gcgtgggtcg tcagcgcgac
      721 gggcctcggc atcgtcggca agcgcgtcat ctaccgcggc cgctcatcgc acgcgggttc
      781 gtcgccggag cgggggcgca acgcgctgag cggcgtcatc cgcctcttca ccggcatcga
      841 cggctggcgt cagcagctgc cgactgattc gcgagtccac ggcatcatcg acttcggtgg
      901 cgacgcggtg aacgtggtcc cctcgcgcgc cgaggcgcgc ttcggactcc gggcgcgaga
      961 cctcgacacg ctccgcgaga tggaggtcgc cttcaccaat gtcgcgcagg gcgcggcgct
     1021 cgagaccagc acgaccgtgg agatcagcga ctacctcccg ctctacgcgc cggtcgcggc
     1081 gaacgcgacg cttggcgacg ccgtcgccgc cgagctgcgc gctcgcggtg tcgagcccga
     1141 gcgcgggacg ctggtgaccg cgtcgaccga tctcggaaac gtgtcgcagc gggtgccgac
     1201 ggactggatc cgcttcccgg tctccgagca cgcgatcccc ggccactccg acgagatgcg
     1261 cgatgcctgt gtcacggacc gtgcgcatgc gaacgcgatc gtcacgatgg aggcactcgg
     1321 cgccgcggct cggcgcgtcg cgacggacgg gcggcttcgt gcgagcatcg cggcatgagc
     1381 gccagtctgc gcgacgacct catcgtcctg tcgcgcgcga tccacggcac gcccgagctc
     1441 gcgtaccagg agcgccaggc cgtcgcgaac atcaccgcgc tactgcgccg tcacggtcac
     1501 gacgtcgaag cgaagctcgg cggggtcgag accgccttcc gcgcgcacct cggtccgaag
     1561 ggaccgtcgg tcgcgctcct cgccgagtac gacgcgctgc ccgagatcgg acatgcctgc
     1621 ggccacaacc tcatcgcgat gtcgaacgtc ggcgcgttcc tcctggccgc ccagcgtgag
     1681 agggacctcc aggtgggcat cgagctgatc ggcacgcccg cggaggagtc cggcggcggc
     1741 aagatcgacc tcatcgacgc cggcgtcttc cgggagtcgg tcgccgtgct ctcgtcgcat
     1801 cccggtggca atgaatggga gctcggcgga tcgagcctcg gcatcgtcgg caagctggtg
     1861 cgcttccacg gcgtcgcatc gcacgccgcg tattcgccgc acaaggggcg caacgcgctc
     1921 accgccgcga tccgcctctt cctcgcggtc gacggctggc ggcagcacct cccggcggac
     1981 acccgcgtgc acgggatcat caccaagggc ggcgcggcgt cgaacatcat cccggatctc
     2041 gccgagtgcg acttcggcct ccgcgccgcg tcgctcgact acctcgagga gatggtcgcg
     2101 acgttccggc agatggccga gggcgcggcg atgcagaccg ggaccaccgt cgaggtcgac
     2161 gacaagatgc gcctgtacgc gccggtcagg cccaacgcga agatcggcga cctgctcgtc
     2221 gcggagctcg cgaagcggag gctcgaggtg gtgcgcggcc ggctcacgct ggcgtccacg
     2281 gacttcggca acgtctcgca agtcgcgccc gcggactaca tcggcttccc ggtgagcacc
     2341 gagcccgtgc ccggccactc gcaccagatg cgcgagtcgt cggcgagcga ctacgcgcac
     2401 gacgcggcga tgaccacagc cgaggtcctc gcgacggtgg cggtccgcat cgcgaccgat
     2461 cccgcggtcc gcgcgtcgct gcgagcgtga acagcaacga cacggtccat cccgtcccgt
     2521 tgacggacat cgcggccgca cgctggctcg acgtcgactt ccccgggctc gagttcggca
     2581 tcgccgagta cgcggagggt ccgaccggct gcaccgtgtt ccatttcccg gagctcgcgt
     2641 cgatgcacat cgacgtgcgc ggcggctcgc cgggtatcaa cggcgagcac ctcacgctga
     2701 cgaacgcgat ctgcttcgcc ggcggctcgc tgtacgggct cgaggcatcc accggcgtcg
     2761 cggccgagct cttcgcgagg cgcggctact ccacgaagtg gatggacatc gccctggtct
     2821 cgggcgcgat catcttcgac tacggccggc gcgagaacac tgtctacccg gacaaggagc
     2881 tggggcgtgc cgcggtgcgc gcgttccggc ccggccggtt cccactcggt ccgcatggcg
     2941 cgggcatctc cgcgaccgcc ggccacggcc ggaccgcgga gctgtcgggg cagggaggcg
     3001 cctggcgtcg ggtcggtgcg acgcgcgttg ccgtgttcac cgtcgtcaac gcgtacggcg
     3061 cgatcatcga ccgcaccggc agcgtcctgc gtggcaaccg cgatccgcac acggggacgc
     3121 gctcacgcgc cgagcgcatg atcgagacga ccggcgctta cgaccctccg ccggggcgca
     3181 tccgcggcgt cagccagaac accaacctga cgctcgtcgt cacggaccag aagctggacc
     3241 cgcttcagct gcgctccgtc gcgcggcaga cacactcctc gatggcccgc gcgatccagc
     3301 cgttccacac ccgctgggac ggcgacatcc tcttcgccgt ctcgacgcag cgcgtggcca
     3361 acgccgcgct cgacgaggtc agcctcgccg tgatcgcatc ggacctcgcc tgggacgcgg
     3421 tcctcgcttc gttcgatccg tagcgggaca tggccgtaac gaccgtgcgc tctcgccagc
     3481 gcgcacgagc ccgcgcacat ccacatcaag gggaaaggtg gccgtgcgaa gtgctggcta
     3541 cggcccgttc gactcgcgtc atcgacctcc actcgaccgg tgacgcgtga catcgagctg
     3601 ctcgtccggc ggcacgaagc ggtgctctgc gacctctggc ggc
//
LOCUS       DASTZR010000305         4568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1009671, whole genome shotgun sequence.
ACCESSION   DASTZR010000305 DASTZR010000000
VERSION     DASTZR010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009671"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="VFV20_06850"
     CDS             <1..933
                     /locus_tag="VFV20_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU5288101.1"
                     /translation="AVTTISRAVRRARAGLKDPRRPIGVFFFVGPTGTGKTHLARQLA
                     EFMIGSQENVVKIDMSDYMERHNVSRLVGSPPGYVGYEEGGQLTETVRRKSYCVILLD
                     EIEKAHPEVFNILLQIFDEGRLADAKGRTVDFRNTIIIMTSNLAQKMLKSESALGFRT
                     HGAGEDADVQAQYERMKERVQEEMKRFFRPEFLNRIDATVIFQPLNKDEIRKIVELEL
                     AGVRKQLLSQEITLDVTTEAKDHIASVGYDSNFGARPLARSIQNEIADPLAEMLLQGK
                     FHPGDTVRVHPVDGKLTIERTEAVEAPKPEPVAP"
     gene            934..1803
                     /locus_tag="VFV20_06855"
     CDS             934..1803
                     /locus_tag="VFV20_06855"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5288102.1"
                     /translation="MPGAPALRLVRSDDPAAFERSAGSFLALREAENNLPLGLISGLK
                     AGRTFGADPPYFAVVHAGSAVVGAAMRTPPFNLILAAGTAPDALRLLVADALSAMPDT
                     PGLVGPKELVARGASIWCDRTGARAEVTMAQRIYRLAHVIPPRDTPGRARVASDADRD
                     TVVPWFQAFVAEAMPGHDASRERAEATADHFLRTRGLWLWEDGAPVAMAGVSGRTPSG
                     ARVGAVYTPPDRRRRGYASALVAALSQAQLDGGARFCFLYTDLANPTSNKIYQDIGYE
                     PVCDVDEYRFSTA"
     gene            1864..3228
                     /gene="radA"
                     /locus_tag="VFV20_06860"
     CDS             1864..3228
                     /gene="radA"
                     /locus_tag="VFV20_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017419468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEU5288103.1"
                     /translation="MPAAPKTAFVCQSCGNQFPKWIGRCPSCHGWNTLVEERVVAPPK
                     GRGTPRAPREAIPLDAVPADAEARISTGIGEFDRVLGGGVVRGSLVLLGGDPGIGKSS
                     LLMQASASLATRGTVLYVSGEESAAQIKMRARRFGIESPNILVLAETDLGTVMEAIRR
                     IKPIFTVIDSIQTMSSEAIASAAGGVSQLRECAARLLEVAKGDDVPIFLVGHVTKEGA
                     VAGPRVLEHIVDAVLYLEGERFHAFRILRGTKNRFGSTDEVGVFEMGESGLREVANPS
                     EVFLEERSKGVAGSVIVPTVEGTRPLLVEVQALVTPTSFGLPRRTVNGADPQRLAVLL
                     AVLAKRAGIGLGQHDVYVNLVGGLRVREPAADLAIAVALASALRDKPADPRTVFVGEL
                     GLGGELRRVQRVAARLKEAQRLGFTRAVVPQASLADLNGTPLEVVGAPTLREALTRSG
                     IADT"
     gene            3362..4438
                     /locus_tag="VFV20_06865"
     CDS             3362..4438
                     /locus_tag="VFV20_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HEU5288104.1"
                     /translation="MSFIRLFIALIGWFFGFFLGASLLEISQVTEYSNRLLVVILLAA
                     ACAILGWLGAPYITIVPARAITRRIHDASAGDLVGGAFGTGIGLMLALFLAFPLSFLP
                     DNLGRFAPLIGAVVLGLGGGAAGTIKRAELAAIVRDIRHGRQDRLGQERALLDTSVII
                     DGRVADIVKSGFIRGTLVVPRFILGELQHFADSSDGTKRERGRRGLEILQKMQKEATV
                     RIELTDADPPGAGADGKLVILARQMSVPIMTNDYGLNRVAELQGVTVLNVNDLAKAVR
                     PVVLPSDDLTVRVIQEGKEPGQGVAYLEDGTMVVVENGARFQGGPEIGVTVMRVLQTV
                     GGRMIFAQPKGEVIEIRRPRPQSH"
BASE COUNT          675 a         1626 c         1584 g          683 t
ORIGIN      
        1 gcggtcacga cgatctcacg cgccgtccgc cgcgcgcgcg ccggcctgaa ggacccgcgc
       61 cgtccgatcg gcgtgttctt cttcgtcggc cccaccggta ctggcaagac ccacctcgct
      121 cgccagctcg ccgagttcat gatcggcagc caggagaacg tggtgaagat cgacatgtcc
      181 gattacatgg agcggcacaa cgtgtcgcgc ctggtcggat cgccgcccgg ctacgtcggg
      241 tacgaagagg gcggccagct caccgagacc gtgcgccgca agtcgtactg cgtgatcctg
      301 ctcgacgaga tcgagaaggc gcaccccgag gtcttcaaca tcctgctgca gatcttcgac
      361 gagggccgtc tcgccgacgc gaagggccgc accgtcgatt tccgcaacac gatcatcatc
      421 atgaccagca acctcgcgca gaagatgctc aagagcgagt cggcactcgg cttccgcacg
      481 cacggcgcgg gcgaggacgc cgacgtgcag gcgcagtacg agcgcatgaa ggagcgcgtg
      541 caggaagaga tgaagcgctt cttccggccc gagttcctga accgcatcga cgcgaccgtg
      601 atcttccagc cgctgaataa ggacgagatc cgcaagatcg tcgaactcga gctcgcgggc
      661 gtgcgcaagc agctcctgtc gcaggagatc acgctcgacg tgaccaccga ggcgaaggac
      721 cacatcgcgt cggtcgggta cgactcgaac ttcggcgcgc ggccgctcgc ccgctcgatc
      781 cagaacgaga tcgccgaccc gctcgcggag atgctgctcc agggcaagtt ccacccgggc
      841 gacaccgtcc gcgtgcatcc ggtcgacggg aagctcacga tcgagcggac cgaggccgtc
      901 gaggcgccga agccggagcc ggtcgcgccc tagatgcccg gcgctccggc gctgaggctc
      961 gtgcgatccg acgaccccgc cgcgttcgag cgatccgccg gctcattcct cgcgctgcgc
     1021 gaggccgaga acaacctgcc gctgggtctg atctcgggtc tcaaagccgg ccgcaccttc
     1081 ggcgcggatc cgccgtactt cgcggtcgtg catgcgggaa gcgcggtggt gggcgcggcg
     1141 atgcggacgc ccccgttcaa cctcatcctc gccgccggta ccgcgcccga cgcgctccgg
     1201 ctgctggtcg ccgacgcgct ctcggcgatg ccggacacgc ctggcctcgt cggtccgaag
     1261 gagctcgtcg cgcgtggcgc ctcgatctgg tgcgaccgca ccggagcgcg tgccgaggtc
     1321 acgatggcgc agcgcatcta ccgcctcgcg cacgtcatcc cgccccgcga cacaccgggc
     1381 cgcgcccgtg tggcgagcga tgcggaccgc gacaccgtcg tgccctggtt ccaggccttc
     1441 gttgccgagg cgatgcccgg ccatgacgcc tcacgcgagc gagcggaggc gaccgcggat
     1501 cacttccttc gcacgcgtgg cctgtggctg tgggaggacg gcgcgccggt cgcgatggcc
     1561 ggcgtcagcg gacggacgcc gagcggtgcg cgtgtgggcg ccgtgtacac gccgcccgat
     1621 cggcggcggc gcggctacgc gagcgcgctc gtcgcggcgc tctcgcaggc gcagctcgac
     1681 ggcggcgcgc ggttctgctt cctgtacacc gacctggcga acccgacctc gaacaagatc
     1741 taccaggaca tcggctacga gccggtctgc gacgtggacg agtaccgctt ctccaccgcg
     1801 taggcgagcg gttatcactt cccggccctc gccgctgtca acctctggcc gatacttggg
     1861 cggatgcccg ccgcgccgaa gaccgcgttc gtctgccaga gctgtggcaa ccagttcccg
     1921 aagtggatcg ggcgctgccc gtcgtgccat ggctggaaca cgctcgtcga ggagcgcgtc
     1981 gtcgcgccgc cgaaaggacg tgggacaccg cgcgcgccgc gtgaggcgat cccgctcgac
     2041 gcggtgcccg cggatgccga ggcgcggatc tccacgggca tcggcgagtt cgaccgcgtc
     2101 ctggggggcg gtgtcgtccg cggatccctc gtgctcctcg gcggcgatcc gggcatcggg
     2161 aagtcgtcgc tgctgatgca ggcgtccgcg tccctcgcga cccgcgggac cgtcctgtac
     2221 gtgtccggcg aggagtccgc ggcgcagatc aagatgcgtg cgcgccgctt cggcatcgag
     2281 agcccgaaca tcctcgtgct cgcggagacc gacctcggca cggtcatgga ggcgatccgc
     2341 cgcatcaagc cgatcttcac ggtgatcgac tcgatccaga cgatgtcgag cgaggccatc
     2401 gcttccgccg cgggcggcgt gtcgcagctc cgcgagtgcg cggcgcggct gctggaggtc
     2461 gcgaagggcg acgacgtgcc catcttcctc gtcggccacg tgaccaagga aggcgcggtc
     2521 gccggaccgc gcgtgctcga gcacatcgtc gacgcggtgc tgtatctcga aggcgagcgt
     2581 ttccacgcct tccgcatcct ccgcgggacg aagaaccgct tcggctccac cgacgaggtc
     2641 ggcgtgttcg agatggggga gagcgggctg cgcgaggtcg ccaatccgtc ggaggtcttc
     2701 ctcgaggagc gcagcaaggg cgtcgcgggc agcgtcatcg tcccgacggt cgagggcacg
     2761 cgcccgctcc tcgtcgaggt gcaggcgctc gtcacgccga cgtccttcgg gctcccgcgg
     2821 cgcacggtca acggcgcgga cccgcagcgg ctcgcggtcc tgctcgcggt gctcgcgaaa
     2881 cgggcgggca tcggcctcgg ccagcacgac gtgtacgtca acctcgtggg cgggctgcgg
     2941 gtccgcgagc ccgccgcgga cctcgcgatc gccgtggcgc tcgcatcggc gctgcgcgac
     3001 aagcccgcgg acccgcgtac cgtcttcgtc ggggagctcg gcctgggcgg cgagctgcgc
     3061 cgcgtgcagc gcgtcgccgc gcgcctgaag gaggcgcagc ggctcggctt cacccgcgcg
     3121 gtcgtgccgc aggcgagcct cgccgacctc aacggaacgc ccctcgaggt ggtcggcgcg
     3181 ccgaccctgc gcgaggcact cacccgttcg ggtatcgcgg acacctaggc acgcgcccct
     3241 agactcgtcg ggtgatccac ctcgtgtcat cccgccgcac cgccgacgtc ggcgaccaca
     3301 tcttcatcgg tcggacgctg ggcgggccgc gcctcgagaa cgagagaaag gagtggacag
     3361 cgtgtcgttc atccgcctct tcatcgcact catcggctgg ttcttcggct tcttcctcgg
     3421 cgcctcgctg ctcgagatct cgcaggtcac cgaatactcc aatcgactgc tcgtcgtcat
     3481 cctgctcgcc gcggcctgcg cgatcctcgg ctggctcggc gcgccgtaca tcacgatcgt
     3541 ccccgcgcgt gcgatcactc gtcgcatcca cgacgcatcc gcgggcgatc tggtcggcgg
     3601 cgcgttcggt accggcatcg gtctcatgct cgcgctcttc ctcgcgttcc cgctctcgtt
     3661 ccttccggac aacctaggga ggttcgcgcc gctcatcggc gcggtcgtcc tcggtctggg
     3721 cggcggagcc gcgggcacga tcaagcgcgc cgagctcgcg gccatcgtgc gggacatccg
     3781 ccacgggcgg caggaccgtc tgggtcagga gcgcgcgctc ctcgacacgt cggtgatcat
     3841 cgacggacgc gtcgccgaca tcgtgaagag cggcttcatc cgaggcacgc tcgtcgtgcc
     3901 gcggttcatc ctcggagagc tgcagcactt cgccgactcc tccgacggca cgaagcgcga
     3961 acgcgggcgc cgcggcctcg agatcctgca gaagatgcag aaggaggcga cggtgcgcat
     4021 cgagctcacc gacgcggacc cgccgggcgc cggcgccgac ggcaagctcg tcatcctcgc
     4081 gcggcagatg agcgtgccca tcatgacgaa cgactacgga ctgaaccgcg tcgccgagct
     4141 gcagggcgtg accgtgctca acgtgaacga cctcgccaag gccgtgcgac cggtcgtgct
     4201 ccccagcgac gacctcaccg tgcgcgtcat ccaggagggc aaagagcccg gccagggcgt
     4261 cgcgtatctc gaggacggga ccatggtggt cgtcgagaac ggggcgcgtt tccagggcgg
     4321 cccggagatc ggggtgacgg tgatgcgcgt gctgcagacc gtcggcggac gcatgatctt
     4381 cgcccagcca aagggcgagg tgatcgagat ccgcagaccg agaccgcaga gccactagtg
     4441 cgccgcgtcc tcgcgatcct cgctgcgggc agcgcgacgc gggcgggcac cgacaaggtg
     4501 ctcgcggagc tcggtggcga gcccgtgctg gcgtggtcgc tcgcggccgg tgtcgccgcg
     4561 ggttgctt
//
LOCUS       DASTZR010000306         2013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_196347, whole genome shotgun sequence.
ACCESSION   DASTZR010000306 DASTZR010000000
VERSION     DASTZR010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196347"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="VFV20_06870"
     CDS             <1..834
                     /locus_tag="VFV20_06870"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5288105.1"
                     /translation="VAKPIEMSVLAAKIDVLLKRVRATAGELVKRRGRVLVFAHGKGG
                     AGTTTLAVNASVALAETKLYRVALLDLNLEFANAHVHLDLKPGKTLVDLAGLDPSQVD
                     DGTFAPLMSQDRSGVQLVMGANAPEDAELVTVPLVQQSLDRLRAAMDYVMVDTPPTFA
                     QHVLVAVDASDGIIVVAEPHLAALEAAKDWLDVLEKLSYPRERCLLVLNRTTQSGLET
                     DQVARFFNRRPEVVVPFTPMFDEAADRGRPLVVLRPENAAAKVMRDLAAQLTVLAPAG
                     R"
     gene            953..>2013
                     /locus_tag="VFV20_06875"
     CDS             953..>2013
                     /locus_tag="VFV20_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5288106.1"
                     /translation="MLDCGTGARKLGLSLATKGPITAHLLLSHTHGDHIQGLPFFLPA
                     FTPGSHLTIYGPAGMDRSLSQTVGGSMDYAYFPVPLSSLPARFDFVQLGETEFTIGRA
                     RVRTQFVNHTSPTIGYRITAGAATLVYATDHEAHANPPWRADRPIGLFDPRYLAHTGD
                     QRHVAFVARADVLVHDTQYAGRDLPAKAGWGHSTVEYAVDVALAGRVKTLVLFHHDPD
                     RDDAGIDEMIRMAEARVAAAGLPLRVLAATEGEELAIDEGATAPVIELEPAAPVLPAR
                     ARILVADDDVTLVRILETVLTGDGYDVDPAYDGTDAVAKAMARRYDLVLMDIAMPQLD
                     GLSACRQLRAAEGYRSTPF"
BASE COUNT          292 a          734 c          687 g          300 t
ORIGIN      
        1 gtggccaagc cgatcgagat gagcgtgctg gcggcgaaga tcgacgtgct gctgaagcgc
       61 gtgcgggcga ccgcgggtga gctcgtgaag cgccgcggcc gcgtcctcgt cttcgcccac
      121 ggcaagggcg gcgcggggac gacgacgctc gcggtgaatg cctcggtcgc gctcgccgag
      181 accaagctgt atcgcgtcgc gctgctcgac ctcaacctcg aattcgccaa cgcgcacgtc
      241 catctggatc tgaagccggg caagacgctc gtcgacctcg cggggctcga cccgtcgcag
      301 gtcgacgacg gcacgttcgc gccgctcatg tcgcaggacc gcagcggcgt ccagctggtg
      361 atgggcgcga atgcgccgga agacgccgag ctcgtgacgg tcccgctggt gcagcagagc
      421 ctcgatcgcc tgcgcgcggc gatggactac gtgatggtcg acacgccacc gaccttcgcg
      481 cagcacgtcc tggtggcggt cgacgcctcg gacggcatca tcgtcgtcgc ggagccccac
      541 ctcgccgcgc tcgaggcggc gaaggactgg ctggacgtgc tcgagaagct ctcgtacccg
      601 cgcgagcgct gcctcctggt gctgaaccgc acgacgcagt cggggctcga gacggatcag
      661 gtcgcccgat tcttcaaccg ccgtcccgag gtggtcgtac ccttcacgcc gatgttcgac
      721 gaggccgccg accgtgggcg gccgctggtg gtgctgcggc ccgagaacgc ggcggcgaag
      781 gtcatgcgcg atctcgccgc acagctgacc gtgctcgcac cggcgggtcg ctgacatccg
      841 cgtccgtttc tggggcaccc ggggctccat cgcggcggcc gggccggaga ccctccgctt
      901 cggcggcaac acgccgtgcg tcgaggtcgt caccgacgac ggagcgcagc tcatcctcga
      961 ctgcggtacc ggcgcgcgaa agctcgggct ctcgctcgcc acgaagggcc cgatcacggc
     1021 gcacctcctg ctgtcccaca cgcacggcga tcacatccag ggcctgccct tcttcctgcc
     1081 ggcgttcacc cccggcagcc atctgacgat ctacgggccc gcgggcatgg accgttcgct
     1141 ctcgcagacc gtcgggggat cgatggacta cgcgtacttc cccgtgccgc tctcctcgct
     1201 gccggcgcgc ttcgacttcg tccagctcgg cgagacggag ttcaccatcg gacgcgcgcg
     1261 cgtgcgcacg cagttcgtga accacacatc gcccaccatc ggctaccgca tcacggccgg
     1321 ggccgcgacc ctcgtgtacg cgaccgacca tgaggcgcac gcgaaccccc cgtggcgagc
     1381 ggaccgtccc atcggtctct tcgatccgcg gtacctcgcg cacaccggcg atcagcggca
     1441 cgtcgccttc gtcgcgcgcg ccgacgtgct ggtgcacgac acccagtacg ccggtcgcga
     1501 tctgccggcc aaggcgggct ggggccacag caccgtcgaa tacgccgtcg atgtcgcgct
     1561 cgcgggacgg gtgaagaccc tggtgctctt ccatcacgat ccggatcgcg acgacgcggg
     1621 catcgacgag atgatccgga tggcggaggc gcgcgtcgcc gcggcgggcc tgccgctgcg
     1681 agtgctggcg gccaccgagg gcgaggagct cgcgatcgac gagggcgcga ccgcgccggt
     1741 catcgagctc gagccggccg cgcccgtgct cccggcccgc gcgcggatcc tcgtcgcgga
     1801 cgacgacgtc acccttgtcc gcatcctcga gaccgtgctg accggcgatg gctacgacgt
     1861 cgatccggca tacgacggca cggacgctgt tgcgaaggcc atggcccggc ggtacgacct
     1921 cgtgctcatg gacatcgcca tgccgcagct cgacgggctt tcagcatgcc ggcagcttcg
     1981 cgcggcggag ggctaccgtt cgacgccttt cat
//
LOCUS       DASTZR010000307         2224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_461442, whole genome shotgun sequence.
ACCESSION   DASTZR010000307 DASTZR010000000
VERSION     DASTZR010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461442"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VFV20_06880"
     CDS             complement(<1..331)
                     /locus_tag="VFV20_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5288107.1"
                     /translation="MIAELNGQLGLAYAFVEREIALSKRYWAWEVVWLIYGIVTSLSV
                     TYIGLAAPAITGGTVDEAAVSRFVLYLLVGTIAWRFLGIIFDNISEVIAWERWEGTIE
                     YTFMSPVP"
     gene            complement(328..1161)
                     /locus_tag="VFV20_06885"
     CDS             complement(328..1161)
                     /locus_tag="VFV20_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5288108.1"
                     /translation="MAIETATRPRSPDLRPPSRDALVAEGVTKTFRTRGESRRAVDDV
                     SLRVERGEIYGLLGANGSGKSTLIRMFSTLLVPDAGEVTVFGLSVGRDENAVKRLINR
                     VSVEASFMKKLSAMENLVYVGRLYGVPPEESRSKSLAILRRLGIAEERVNEPLEKLSR
                     GMQQKVAIARAFLTAPMLLLLDEPTTGLDIRSKREVQTFVRDLRLEHDATILLTTHDL
                     DEAERLADRIGILHDGRLVAEGTADELRARYGASAIEDVFMTATGRRLDEADATEGAR
                     R"
     gene            complement(1435..1587)
                     /locus_tag="VFV20_06890"
     CDS             complement(1435..1587)
                     /locus_tag="VFV20_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEU5288109.1"
                     /translation="MLWTIVLVLLILWLLGVVALPSVGALIHLLLVLALIVLVVQLLS
                     GRRVAL"
     gene            complement(1793..>2224)
                     /locus_tag="VFV20_06895"
     CDS             complement(1793..>2224)
                     /locus_tag="VFV20_06895"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEU5288110.1"
                     /translation="VFTADLKSSNEVPAIADAEASCAGKGTFTLNTTKDSAGLITAAT
                     AQFDLTITGCPAGTEITLFHIHKQVAGQNGGVVIDSGQKAAEPIVLATGATSGTVTKS
                     SITVKPEDAKAIIDGPSGFYFNVHSKLHGGGVVRDQLKASS"
BASE COUNT          380 a          747 c          760 g          337 t
ORIGIN      
        1 gaggcacggg cgacatgaac gtgtactcga tcgtgccctc ccagcgttcc cacgcgatga
       61 cctcgctgat gttgtcgaag atgatcccga ggaaccgcca cgcgatcgtg ccgacgagca
      121 gatagagcac gaagcgcgag accgccgcct catccaccgt gccgcccgtg atcgcgggcg
      181 cggcgagacc gatgtacgtg acggagaggg acgtgacgat cccgtagatg agccagacga
      241 cctcccaggc ccagtaccgc ttcgagagcg cgatctcgcg ctccacgaac gcgtatgcca
      301 ggcccagttg gccgttcagc tcggcgatca tcgccgtgcc ccctccgtcg cgtcggcctc
      361 gtcgaggcgc ctacccgtcg cggtcatgaa cacgtcctcg atcgcggacg cgccatagcg
      421 cgcccggagc tcatcggccg tgccctcggc gacgagacgc ccgtcgtgca ggatgccgat
      481 ccggtcggcg aggcgctcgg cctcgtcgag gtcatgcgtc gtcagcagga tcgtcgcgtc
      541 gtgctcgagc cgcaggtcgc gcacgaaggt ctgcacctcg cgcttgctgc ggatgtccag
      601 gccggtcgtc ggctcgtcga ggagcagcag catcggcgcg gtcaggaacg cgcgagcgat
      661 cgccaccttc tgctgcatgc ctcgcgagag cttctcgagc ggctcgttca cccgctcctc
      721 ggcgataccg aggcggcgca ggatcgcgag cgacttcgac cgcgactcct ccggcgggac
      781 gccgtacaac cgtccgacgt agacgaggtt ctccatggcg gacagcttct tcatgaagga
      841 cgcctcgacg gagacgcggt tgatgagccg cttcaccgcg ttctcgtccc gaccgacgct
      901 gaggccgaag acggtcacct cgccggcgtc ggggacgagg agcgtcgaga acatgcggat
      961 gagcgtcgac ttcccggacc cgttcgcgcc gagcagaccg tagatctcgc cgcgctcgac
     1021 gcgcagcgac acgtcgtcga cggcacgacg ggactcgccg cgcgtgcgga aggtcttcgt
     1081 gactccctcg gcgacgagcg cgtcgcgcga aggtggccgc aggtcgggac ttctgggtct
     1141 ggtcgcggtc tcgatggcca ctgctcactc cttcaccgga aataaaaaga ccgtgggttc
     1201 tgcccacggc cggcggtcag gagcgggctc gggccctaga ccatccgagg tgtgggcagg
     1261 ctacgccgcg ggaaacccgc gtcgttgggc gcgatccgga acatcacgag cactgcgcca
     1321 cttcctttca acgagtcacg cgaagcgtgc gaggacgaac gataccaagc gagcgagggc
     1381 ggggtcaact accccgccct cgtcctgtga ctgcgactgc gactcagacc gcgctcagag
     1441 cgcgacgcgt cttccgctca gaagctggac gacgagcacg atcagcgcga gcacgagcag
     1501 caggtggatc agcgcaccca ccgagggcaa cgccaccacg ccgagcagcc agaggatgag
     1561 aaggaccagg acgatggtcc acagcatggc gagcacctcc tttaccaaac cagcggacgc
     1621 aaggtgcgtg ccaccagtgg agcgcccagc ggtcaggcga gccgctcgcc aagatcccca
     1681 tggctggcac aggcaagctc acggcccggg gcgcggatgt ccgtccgcag gaacggcgac
     1741 gcccccgggc ccgaggagag gccggggggc gtcaatgcgg aggacgagcg gtctaggagc
     1801 ttgccttcag ctggtcgcgg accacgccgc caccgtgcag cttcgagtgc acgttgaagt
     1861 agaagcctga gggtccgtcg atgatggcct tcgcgtcttc aggcttcacg gtgatgctcg
     1921 acttcgtcac ggtcccagag gtcgcgcccg tcgcgaggac gatcggctcc gcggccttct
     1981 ggccggagtc gatcacgacg ccgccgttct gaccggcgac ctgcttgtgg atgtggaaca
     2041 gcgtgatctc ggttccggcc gggcagccgg tgatcgtgag gtcgaactgc gccgtcgcgg
     2101 cggtgatgag gccggccgag tccttcgtcg tgttcagggt gaacgtgccc ttgccggcgc
     2161 acgacgcctc ggcgtcggcg atggccggga cctcgttcga ggacttgagg tcggccgtga
     2221 agac
//
LOCUS       DASTZR010000308         3462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_379101, whole genome shotgun sequence.
ACCESSION   DASTZR010000308 DASTZR010000000
VERSION     DASTZR010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379101"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..2465
                     /gene="purL"
                     /locus_tag="VFV20_06900"
     CDS             171..2465
                     /gene="purL"
                     /locus_tag="VFV20_06900"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF002290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEU5288111.1"
                     /translation="MNPSPTVTGTTTAKPKLDISREVGLTPFEAERVRSLTAREPNAL
                     EWAMFGAMWSEHCAYKHSKPLLRTLPTKGPRVAVGPGENAGAVDLGDGLLCAFKVESH
                     NHPSAVEPYQGAATGVGGILRDVFAMGARPIALLNALCFGTPDQPRARRLVGGVVGGI
                     GFYGNCVGIPDVAGHVSFEPCYLDNPLVNAMCVGLVERDRLRFANSARPGSAVYLVGS
                     DTGRDGIAGASLLASFELGSGDDSKRPSVQVGNPFLEKLLMEATLELIECDAVEAVQD
                     LGAAGLTCATSEVAARSGVGMRIDVDVVPRRAERMTPYEVMLSESQERMLIVARPGRE
                     ADIERVFERWELHGVRVGEVVEEPVLTIVSEGAAVATLDPRWLAEDAPEYDVREWAKE
                     PSRAASGSPASGESDSLEPGQRGQAAEPLGRAATRKIGLELLDVLASPNVASRRVLYR
                     TYDQMVGTDTVIAPGGDAGVMRIKGRKDGIAVAIDAQPRVAAIDPFVGAASAVAEALR
                     NLVCAGATPLAITDCLNVGNPERPTGAWQLERTVAGIKAACDALGVPVVSGNVSLYNA
                     TKGADIWPTAVIGAVGHLEDVTRTIPVTSGRAGDAVLLAGSANVGIGTSAYGALAGVH
                     EGPVAIDLALEARLQRFVLAAHSRGLVRAAHDRAEGGLAAALAELVLRDGLGMKVTLP
                     AVRGIDKRVALFGEGPSGIVLVADPKHADALRALAAEHDVPVWTLGTMGGDLLEVAPV
                     LGIPVASLAQAHGHGLARALGREA"
     gene            2468..>3462
                     /locus_tag="VFV20_06905"
     CDS             2468..>3462
                     /locus_tag="VFV20_06905"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU5288112.1"
                     /translation="MCGVCGIWSPVEAARRDPAEAARLMFFSLYALQHRGQESAGIAA
                     SDGRDLRVVRRMGLVGQVFSEPDLSVLGGRTAMGHTRYSTTGSSKIQNAQPFVIHDDE
                     LGELAIGHNGNCINARDIRDQLAAQGVRFVTSSDTEVVAQAIANAAGASWDERIVNAL
                     PRLQGAWSLVMLTPGALYAVRDPLGVRPLCLGRKGASWLVASETSALDTIGAEFIREV
                     APGEVMRIDDRGPVPIADLASADRRGLCVFEHVYLASASSRLGGVSVYATRERMGEIL
                     ADEHPAKADAVIPVPDSAIPAAVGYARRSGIPFHEGLIKNRYIGRTFIEPTQEIR"
BASE COUNT          472 a         1235 c         1275 g          480 t
ORIGIN      
        1 cggccgctgg cgtatcgcct ggagcacctg gcgaacctgc ggaacctgct cgcctacgac
       61 cgcgagcaga ccgacatcga cgacatctac gcgcagatcc agacccgcct caccgacttc
      121 gcgtcgttcg ccgatcaggt cgagcgcttc gtaggagcgg gcgaggacgc atgaacccgt
      181 ccccgacggt gacggggacg acgacggcga agcctaagtt ggacatctct cgcgaggtcg
      241 gcctcacacc cttcgaggcg gagcgcgtgc gttccctcac ggctcgcgaa ccgaatgcgc
      301 tcgaatgggc gatgttcggc gcgatgtgga gcgagcactg cgcgtacaag cactcgaagc
      361 cgctgctgcg cacgctgccg acgaagggcc cgcgcgtcgc ggtcggtccg ggcgagaacg
      421 ccggcgcggt cgacctcggc gacggcctgc tctgcgcgtt caaggtcgag agccacaacc
      481 atccctcggc ggtcgagccg taccagggcg cggccaccgg tgtcggcggc atcctgcgcg
      541 acgtcttcgc gatgggcgcg cggccgatcg cgctgctgaa cgccctctgc ttcggcacgc
      601 ccgaccagcc ccgcgcgcgg cgcctggtcg gcggggtcgt cggcgggatc ggcttctacg
      661 gcaactgcgt cggcatcccc gacgtcgcgg gccacgtctc gttcgagccc tgctacctcg
      721 acaacccgct ggtgaacgcg atgtgcgtcg gcctcgtcga gcgcgaccgt ctgcgcttcg
      781 ccaactccgc gcgcccgggc agcgcggtgt acctcgtcgg ctcggacacg ggacgcgacg
      841 gcatcgccgg cgcctcgctg ctcgcgagct tcgagctcgg ctcgggcgac gactcgaagc
      901 ggccgtcggt gcaggtcggc aacccgttcc tcgagaagct cctcatggaa gcgacgctcg
      961 agctcatcga gtgcgatgcg gtggaggcgg tgcaggacct cggcgcggcc ggcctcacgt
     1021 gcgcgaccag cgaggtcgcc gcgcggagcg gcgtcggcat gcggatcgac gtcgacgtcg
     1081 tgccgcgccg cgcggagcgc atgacgccgt acgaggtgat gctgtccgag tcgcaggagc
     1141 ggatgctgat cgtcgcgcgc cccggccgcg aggccgacat cgagcgcgtc ttcgagcgct
     1201 gggagctgca cggcgtgcgc gtcggcgagg tcgtggaaga gccggtgctg accatcgtga
     1261 gcgagggcgc ggcggtcgcg acgctcgatc cacgctggct cgcggaggac gcacccgagt
     1321 acgacgtgcg tgagtgggcg aaggaaccga gccgagccgc gagcgggtcc cccgcgagcg
     1381 gcgagtccga cagccttgag cccggccagc gaggtcaagc ggcggagccg cttggacgag
     1441 ctgcgacgag gaagatcggg ctggagctgc tcgacgtgct cgcgtcgccg aacgtcgcgt
     1501 cgcgacgcgt gctctaccgg acgtacgacc agatggtcgg caccgacacg gtgatcgcgc
     1561 cgggtggcga tgccggcgtc atgcgcatca agggacggaa ggacggcatc gcggtcgcga
     1621 tcgacgcgca gccccgcgtc gcggcgatcg atccgttcgt cggcgcggcg agcgccgtcg
     1681 ccgaggcgct gcgcaatctc gtctgcgccg gcgcgacgcc gctcgcgatc accgactgcc
     1741 tcaacgtggg caaccccgag cggccgaccg gagcgtggca gctcgagcgc accgtcgccg
     1801 gcatcaaggc ggcgtgcgac gcgctcggcg tgccggtcgt ctcggggaac gtctcgctct
     1861 acaacgcgac gaagggcgcc gacatctggc cgaccgcggt catcggcgcc gtcgggcatc
     1921 tcgaggacgt gacccgcacg atccccgtga cgtcgggtcg cgccggcgat gccgtcctgc
     1981 tcgcgggcag cgcgaacgtc ggtatcggca cgtcggcgta cggcgcgctc gccggcgtgc
     2041 acgagggacc ggtcgccatc gatctcgcgc tcgaggcgcg gctccagcgc ttcgtgctcg
     2101 cggcgcactc gcgcggtctc gtgcgtgccg cgcacgatcg cgccgagggc ggcctcgcgg
     2161 ccgcgctcgc cgagctcgtg ctgcgtgacg ggctcggcat gaaggtgacg ctgccggcgg
     2221 tccgcggcat cgacaagcgc gttgcgctct tcggcgaggg gccctccggc atcgtgctcg
     2281 tcgccgatcc gaagcacgcc gacgcgctgc gggcgctcgc cgcggagcac gacgttccgg
     2341 tctggacgct cggcacgatg ggcggcgatc tgctcgaggt cgcgcccgtg ctcggcatcc
     2401 ccgtcgcgtc gctcgcgcag gcgcacggcc acggcctcgc acgcgccctc gggcgggagg
     2461 cctaggtatg tgcggggtat gcggcatctg gagtcccgtc gaggcggcgc gccgcgatcc
     2521 cgctgaggcc gcgcgcctca tgttcttctc gctctacgcg ctgcagcacc gcgggcagga
     2581 gtccgcgggc atcgccgcgt ccgacggccg cgatctgcgc gtggtccggc gcatggggct
     2641 cgtgggccag gtcttcagtg agcccgacct ttcggtgctc ggcggccgga ccgcgatggg
     2701 gcacacgcgc tactccacca ccggctcgtc gaagatccag aacgcgcagc cgttcgtcat
     2761 ccacgacgac gagctgggcg agctcgcgat cgggcacaac ggcaactgca tcaacgcgcg
     2821 cgacatacgg gaccagctcg ccgcgcaggg cgtgcgcttc gtgacgtcgt cggacaccga
     2881 ggtcgtcgcg caggcgatcg cgaacgcggc gggcgcgtcg tgggacgagc gcatcgtgaa
     2941 cgcgctgccg cgcctgcagg gcgcgtggtc gctcgtgatg ctcacgcccg gcgccctgta
     3001 cgccgtgcgc gacccgctcg gcgtgcggcc gctctgtctc gggcgcaagg gcgcgtcctg
     3061 gctcgtcgcg agcgagacca gcgcgctcga cacgatcggc gccgagttca tacgcgaggt
     3121 cgcgcccggc gaggtcatgc gcatcgatga ccgcgggccg gtccccatcg cggatctcgc
     3181 gtcggccgac cggcgcggcc tctgcgtctt cgagcacgtc tacctcgcgt cggcgtcgag
     3241 ccggctcggc ggcgtgtcgg tgtacgcgac gcgcgagcgg atgggggaga tcctcgccga
     3301 cgagcatccc gcgaaggccg acgcggtgat cccggtcccg gacagcgcca tccccgccgc
     3361 ggtgggctac gcgcgccgga gcggcatccc gttccacgag ggcctcatca agaaccgcta
     3421 catcgggcgc acgttcatcg agccgacgca ggagatccgc ga
//
LOCUS       DASTZR010000309         4483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1192626, whole genome shotgun sequence.
ACCESSION   DASTZR010000309 DASTZR010000000
VERSION     DASTZR010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192626"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VFV20_06910"
     CDS             complement(<1..586)
                     /locus_tag="VFV20_06910"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU5288113.1"
                     /translation="MIGARRGMGGPGGMDPMLLIGLLILVYAFITGSLFAPIRDPIQF
                     AAFAIAIVLGITVHEFMHAYVAHRLGDDTARLLGRLSLNPMAHLDPFGTLLLVLAGFG
                     YGKPVPFNESRLRSALGVTFVALAGPLANLALAAVAAIPLRFGTAEVLGGVYAGILQY
                     VVFMNCVLAIFNLIPIPPLDGSNVVYGLLSPRQQY"
     gene            complement(612..1922)
                     /locus_tag="VFV20_06915"
     CDS             complement(612..1922)
                     /locus_tag="VFV20_06915"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEU5288114.1"
                     /translation="MTRAVELIERKRDGGALTAEEIDTLVQGYVKGEIPDYQMAAFCM
                     AVVWRGMTAKETAALTASMVASGERLDLSRFGRVVDKHSTGGVGDKTTLVVAPLVAAC
                     GLPVAKMSGRGLGFSGGTLDKLESIAGYRVDLTTAEFLAQLERIGIVVTGQTKELAPA
                     DGLIYALRDATGTVPAIPLIASSIMSKKIAAGAHAVVLDVKVGSGAFMKDRRTAAALA
                     RTMVAIGVAHGLGVTCELTDMGQPLGRAVGNALEVAEAIATLRGEGPADLTELALVAG
                     AEMLVRGKRARDVKAARARVERALADGSGLAKLRELISGQSGDARVVNEPSRLPRAPR
                     VETLRAPRTAYVAAIEADRVGLASVHLGAGREKKGEPIDHRTGILLHAKVGDRVERGA
                     PFAEVHVAGKPGDGAAVEEVRSAFRWSARRVSRRKLFLGRIASR"
     gene            complement(1919..2725)
                     /locus_tag="VFV20_06920"
     CDS             complement(1919..2725)
                     /locus_tag="VFV20_06920"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU5288115.1"
                     /translation="MSVDTTAAVAAIGGLTRQKPTVAIVLGSGLGALADEVQDADRIA
                     YERIPGWKRSTVAGHKGQLVIGTLSGKTVAVMQGRLHFYEGYDMHEITFPVRVFRALG
                     IETIILTNACGGLNPSFKNGDLMVVSDHINFMAHNPLRGANDERLGPRFPDMVGVYTE
                     ELRRIAHEVDGDLREGVYVAVAGPNFETPAELRMLRRWGADAVGMSTVPEVIVARHAG
                     MRVLAISTVTDMATGLPGLVAHVSHEEVLAIADSAGTRLGNVVKGVVARL"
     gene            complement(2722..3546)
                     /locus_tag="VFV20_06925"
     CDS             complement(2722..3546)
                     /locus_tag="VFV20_06925"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEU5288116.1"
                     /translation="MKVAVCFRPNFTDAANAAREAATRIRRGGHDVAEVTLDAVRAEQ
                     LAGVELACVFGGDGTMLHAARALAPRGIPLLGVNLGHLGFLTMATIGEFDAAFADVVA
                     GRYEVEERAMLDARLVRGGADVLVAPALNDVAVARGSLVRSIHVAVEVDGDPLIVYWA
                     DGVIVATATGSTAYAFSVGGPLLLPSARSLVLAPIAPHLSFSNSFVFEPDQQIALDVQ
                     DEPARLSIDGQVEHDLRAGDRIAVRRSSAVAKLVRTARARPFLSLLREKILKEPGI"
     gene            complement(3543..4325)
                     /locus_tag="VFV20_06930"
     CDS             complement(3543..4325)
                     /locus_tag="VFV20_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEU5288117.1"
                     /translation="MPRRVRLDQLLVRRSLAPSRERAQALILAGVVRVDGDPVRRSAD
                     TVPDDASVTLDAGPRFVSRGGEKLDAALDELGVDVRGKTALDVGSSTGGFTDCLLQRG
                     ATRVYAVDVGKGQLEWRLRNDARVVVMEGVNAREGVSLPEPVDLIVADVSFISLRLAL
                     PPSFAHLRDGGDVVALVKPQFEAGREAVGRGGIVRDPAARADAVVAVAQHFAREGVPV
                     VAIVPSRVPGREGNREIFVRARKGAAGLTEEATRAAADEASR"
BASE COUNT          683 a         1635 c         1537 g          628 t
ORIGIN      
        1 cgtattgctg gcggggcgac aggagcccgt acacgacgtt cgagccgtcc agcggcggga
       61 tcgggatgag gttgaagatc gcgaggacgc agttcatgaa gaccacgtac tgcaggatgc
      121 ccgcgtagac gccgccgagg acctccgccg tgccaaagcg cagcgggatc gcggcgacgg
      181 cagcgagcgc gagattcgcg agcggccccg cgagcgcgac gaacgtcaca ccaagggccg
      241 atcgaaggcg cgattcgttg aatgggaccg gcttgccata cccgaatccg gccaggacga
      301 gcagcagtgt gccgaacgga tcgaggtgcg ccatcgggtt gaggctgagg cggcccagca
      361 gtcgtgcggt gtcgtcgcca aggcgatgcg cgacgtaggc gtgcatgaac tcatggacgg
      421 tgatgcccaa cacgatggcg atggcaaagg ccgcgaactg aatgggatcg cggatcgggg
      481 cgaagaggga tccggtgatg aacgcgtaga caaggatcag caggccgatg agcagcatcg
      541 gatccatccc acccgggccg cccatgccgc gccgtgcgcc gatcacttgc ccagtctagg
      601 taagaacgac gtcatcgact cgcgatgcgt ccgaggaaga gcttgcggcg cgagacgcgt
      661 cgcgcgctcc agcggaacgc ggagcgcacc tcctcgaccg cggcgccgtc gcccggcttg
      721 ccggcgacgt gcacctccgc gaacggagcg cctcgctcga cacggtcccc caccttcgcg
      781 tgcagcagga tgccggtccg gtggtcgatc ggctcaccct tcttctcgcg accggccccg
      841 agatggacgg atgccaggcc gacgcggtcg gcttcgatcg ccgcgacata cgccgtgcgg
      901 ggcgcgcgca gcgtctcgac cctcggagcg cgcggaagcc gcgacggctc gttcacgacc
      961 cgcgcgtcgc cgctctggcc gctgatgagc tcgcgcagct tggccagccc ggacccgtcg
     1021 gcgagcgccc tctccacgcg cgctcgcgcc gccttcacgt cgcgcgcgcg cttgccgcgc
     1081 accagcatct ccgcccccgc cacgagcgcg agctcggtga ggtccgcggg accctctccg
     1141 cggagcgtcg cgatcgcttc ggcgacctcg agcgcgttgc cgaccgcgcg gccgaggggc
     1201 tgccccatgt ccgtgagctc gcaggtgacg ccgaggccgt gcgccacgcc gatcgcgacc
     1261 atcgtccgcg cgagcgccgc ggcggtacgg cgatccttca tgaacgcgcc gctgccgacc
     1321 ttcacgtcga gcaccacggc atgtgcgccg gcggcgatct tcttcgacat gatgctcgac
     1381 gcgatcagcg ggatggcggg caccgtgccc gtcgcgtcgc ggagcgcgta gatgagcccg
     1441 tccgccggcg cgagctcctt ggtctgtccg gtcacgacga tgccgatccg ctccagctgc
     1501 gccaggaatt cggcggtcgt gaggtcgacg cggtagccgg cgatcgactc gagcttgtcg
     1561 agcgtaccgc cggagaaccc gagcccccgt ccgctcatct tcgcgaccgg gagaccgcag
     1621 gccgccacga gcggcgccac gacgagcgtg gtcttgtccc cgacgccgcc ggtggagtgc
     1681 ttgtccacga ctcggccgaa acgcgagagg tcgagtcgct cccccgacgc gaccatgctc
     1741 gcggtgagcg cggccgtctc cttcgcggtc ataccgcgcc acaccacagc catgcagaac
     1801 gccgccatct gatagtcggg gatctcccct ttgacgtatc cctggacgag cgtgtcgatc
     1861 tcctcggcgg tgagcgctcc cccatccctc ttccgctcga tcagctcgac ggcgcgcgtc
     1921 acagccgcgc gacgacgccc ttcacgacgt tgccgagccg cgtgccggcg ctgtcggcga
     1981 tcgcgaggac ctcttcgtgc gacacgtgcg cgacgagacc ggggaggccg gtcgccatgt
     2041 ccgtgaccgt cgagatcgcg agcacccgca tgccggcgtg acgcgcgacg atcacctcgg
     2101 gcacggtgct catgccgacc gcgtccgcgc cccagcgtcg cagcatccgc agctccgcgg
     2161 gcgtctcgaa gttcggcccg gcgacggcca cgtacacgcc ctcgcgcaga tctccgtcga
     2221 cctcgtgcgc gatccgtcgc agctcctccg tgtacacgcc gaccatgtcc gggaagcgcg
     2281 ggccgaggcg ctcgtcgttg gcgccgcgca aagggttgtg cgccatgaag ttgatgtggt
     2341 cgctcaccac catgaggtcg ccgttcttga agctcggatt caggccgcca catgcgttcg
     2401 tcaggatgat cgtctcgata ccgagtgccc ggaagacgcg caccgggaac gtgatctcat
     2461 gcatgtcgta gccctcgtag aagtgaaggc gcccctgcat gacggcgacg gtcttcccgc
     2521 tgagcgtgcc gatgacgagc tggcccttgt ggcccgcgac cgtgctgcgc ttccagcccg
     2581 ggatgcgttc gtacgcgatg cgatcggcgt cctggacctc gtccgcgagc gccccgaggc
     2641 cgctgccgag cacgatcgcg acggtgggct tctgtctcgt gagcccaccg atcgcggcga
     2701 ccgcggcggt cgtgtccacg ctcatatccc aggctccttc aggatcttct cgcgcaagag
     2761 cgacaggaac ggccgcgcgc gggcggtgcg cacgagcttc gcgaccgcgc tcgagcggcg
     2821 cacggcgatg cggtcaccgg cgcggaggtc gtgctcgacc tgtccgtcga tcgacagccg
     2881 cgcgggctca tcctgcacgt cgagcgcgat ctgctggtcg ggctcgaaca cgaaggagtt
     2941 cgagaaggac aggtgcggcg cgatcggggc gagcaccagc gatcgcgcgc tcggcagcag
     3001 cagcggaccg cccaccgaga aggcgtaggc ggtgctgccg gtcgcggtgg cgacgatcac
     3061 accgtcggcc cagtagacga tgagcgggtc gccgtcgacc tcgacggcca cgtggatcga
     3121 gcgcacgagc gatccacgcg cgaccgcgac gtcgttcagc gcaggcgcca cgagcacgtc
     3181 cgccccaccg cgtaccagtc gagcgtccag catcgcgcgc tcctcgacct cgtaccggcc
     3241 ggcgacgacg tcggcgaagg cggcatcgaa ctcgccgatg gtcgccatcg tgaggaagcc
     3301 caggtgaccc aggttcacgc cgaggagcgg gatgccgcgc ggtgcgagcg cgcgtgccgc
     3361 gtgcagcatc gtcccatcgc caccgaacac gcacgccagc tcgacaccgg caagctgttc
     3421 ggcgcgcacc gcgtcgagcg tgacctccgc cacgtcatgg cccccgcgcc ggatgcgggt
     3481 cgcggcctcg cgcgccgcgt tcgccgcgtc ggtgaagttc ggccggaagc acaccgcgac
     3541 cttcatcgcg acgcctcgtc ggcggcggca cgggtcgcct cctcggtcag cccggccgcg
     3601 cccttccgcg cacgtacgaa gatctcgcga ttgccctcgc gccccggcac acgtgacggg
     3661 acgatcgcga cgaccggtac gccctctcgc gcgaagtgct gcgcgaccgc cacgaccgca
     3721 tccgcacgcg ccgccgggtc gcgcacgatc ccgccgcggc cgaccgcttc gcgcccggcc
     3781 tcgaactgcg gcttcaccag cgcgacgacg tcgccgccgt cgcgcaggtg cgcgaacgac
     3841 ggcgggagcg cgagacgcag cgagatgaac gacacgtcgg cgacgatgag gtccacgggc
     3901 tccgggagcg acaccccttc gcgggcattc acgccctcca tcacgacgac gcgcgcgtcg
     3961 ttgcggagcc gccactccaa ttgccccttg ccgacgtcga cggcgtagac gcgcgtcgct
     4021 ccgcgttgca gcaggcagtc ggtgaagccg cccgttgacg acccgacatc gagcgcggtc
     4081 tttcctcgca cgtcgacacc gagctcgtcg agcgccgcgt cgagcttctc gcctccgcgg
     4141 gagacgaagc gcggacccgc gtcgagcgtg acgctcgcgt cgtccgggac cgtgtccgcg
     4201 ctgcgccgga ccggatcgcc gtcgacgcgc accacgccgg cgaggatgag cgcctgcgcg
     4261 cgctcgcggc tcggcgcgag cgagcgccgc acgaggagct gatcgagacg tacgcggcgg
     4321 ggcacctgtg gccgattctc gcgcatttcg catactgcga cgcgatggcc aagcgtcttc
     4381 cgcgcagccg gaccctgaag ggggacccga gcctctcgta cgtcgacagc ggtcccggca
     4441 cgccgggaga gccggcgatg ctcctcatgc acggctcgct ctc
//
LOCUS       DASTZR010000310         2255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_957877, whole genome shotgun sequence.
ACCESSION   DASTZR010000310 DASTZR010000000
VERSION     DASTZR010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957877"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2255
                     /locus_tag="VFV20_06935"
     CDS             <1..>2255
                     /locus_tag="VFV20_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEU5288118.1"
                     /translation="SLDDPDALPNVIEALRKGIEKVISTLGMHELRGYGRQLSAIGLA
                     PDVARLLGIRTFYGAHDRGLTWERLAQDGLERAMALRARSTARVEPPFRVYPRIWKTA
                     LQVARGEAPYGAYAEKLGEIEREHPVSLRHLVDLARPVDGEPERRAVTKAGEHAAPMY
                     ISSMSFGSQGETAYRAYAEAMARMDMLCINGEGGELPDLIGKYPRNRGQQIASGRFGV
                     SALLANSSDYLEIKIGQGAKPGEGGHLPARKVSVKVALARNAKPGVDLISPSNNHDIY
                     SIEDLAQVVHELKTVNPRARVAVKVPVVPDIGIIAVGIAKSGADIVTLSGYDGGTGAA
                     RQHALRRAGLPAEIGVSEAHRALVAAGLRDEVEVWCDGGMKSALDVVKMLCLGADRVG
                     FATLAMVSIGCTICRGCQLDTCHVGIATQIESALEATERGLKRFEPQEFERAVEQLCR
                     FFAQMKDELARIAGHLGVARTEELVGRTDLLVQARGLERVDLSALLAPAGPMRHGGRE
                     LRVLAAPGPGARAVSEAGAPALHVRLGSEHRFVATSTAGQLARQAIAGSVVTSFEGAY
                     GDGSVAGNGLGAYSTDGVSLVIQGGAQDGALKTALGGSVAVLKARNAGGAWVDGSVGK
                     CFAYGAQRGRFFVQGGADARAGIRLSGADVVFGGDLRGFAFEYMTGGRAVVLGDPGRW
                     ICSGMSGGVVFLRHDPARGMDERALRDRFAKGAKVSLRVPDEGDAAALGELLGAYATA
                     LRDSGQDNASR"
BASE COUNT          300 a          806 c          835 g          314 t
ORIGIN      
        1 gctcgctcga cgaccccgac gcgctcccga acgttatcga ggcgctgcgc aagggcatcg
       61 agaaggtgat ctcgacgctg ggcatgcacg agctgcgcgg ctacggccgc cagctctccg
      121 ccatcggcct cgcgcccgac gtcgcgcggc tgctcggcat ccgcaccttc tacggcgcgc
      181 acgaccgcgg cctcacgtgg gagcggctcg cgcaggacgg gctggagcgc gccatggcgc
      241 tccgtgctcg cagcaccgcg cgcgtcgagc cgccgttccg cgtgtacccg cgcatctgga
      301 agaccgcgct gcaggtcgcg cgcggagagg cgccgtacgg cgcgtacgcg gagaagctcg
      361 gcgagatcga gcgcgagcat ccggtgtcgc tccgccatct cgtcgacctc gcgcgtcccg
      421 tcgatggcga gccggagcgt cgcgccgtga cgaaggcggg cgaacacgcc gcgccgatgt
      481 acatctcctc gatgtcgttc ggttcgcagg gcgagacggc ctaccgcgcg tacgccgagg
      541 cgatggcgcg catggacatg ctctgcatca acggcgaggg cggcgagctg cccgacctca
      601 tcggcaagta cccgcgcaat cgcgggcagc agatcgcgtc cggccggttc ggcgtgtccg
      661 cgctcctcgc caactcgtcc gattacctcg agatcaagat cggccagggc gcgaagcccg
      721 gtgagggcgg ccaccttccg gcgcgcaagg tctcggtgaa ggtcgcgctc gcgcgcaacg
      781 cgaagccggg cgtcgacctc atctcgccgt cgaacaacca cgacatctac tcgatcgagg
      841 acctcgcgca ggtcgtccac gagctcaaga ccgtgaatcc ccgcgcgcgc gtcgcggtga
      901 aggtgccggt cgtgccggac atcgggatca tcgcggtcgg gatcgcgaag tctggcgccg
      961 acatcgtcac gctgtcgggc tacgacggcg gcaccggcgc ggcgcgacaa cacgcgctgc
     1021 gtcgcgccgg gctccccgcg gagatcggtg tgtccgaggc gcaccgcgcg ctggtcgcgg
     1081 cgggtctgcg cgacgaggtc gaggtctggt gcgacggcgg catgaagagc gcgctcgacg
     1141 tcgtgaagat gctgtgtctg ggtgccgatc gtgtgggctt cgcgacgctc gccatggtct
     1201 cgatcggctg cacgatctgc cgcggctgcc agctcgacac ctgccatgtc ggcatcgcga
     1261 cgcagatcga atccgccctc gaggcgacgg agcgcggcct gaagcggttc gagccgcagg
     1321 agttcgagcg ggccgtcgag cagctctgcc gcttcttcgc gcagatgaag gacgagctcg
     1381 cgcgcatcgc cggacatctc ggcgtcgcgc gcacggagga gctggtcggc cgtaccgatc
     1441 tcctcgtgca ggcgcgcggc ctcgagcgcg tcgacctgtc cgcgctcctc gcgccggcgg
     1501 gcccgatgcg gcacggcggt cgtgagctgc gcgtgctggc cgcgcccgga cccggcgcgc
     1561 gtgccgtcag cgaagcgggg gcgccagccc tgcacgtgcg actcggcagc gaacaccgct
     1621 tcgtcgcgac ctcgaccgct ggccagctcg cgcgccaggc gatcgccggc agcgtcgtga
     1681 cgagcttcga gggcgcgtac ggcgacggct cggtggccgg caacgggctc ggtgcgtact
     1741 cgaccgacgg cgtctcgctg gtcatccagg gcggcgcgca ggacggcgcg ctgaagaccg
     1801 cgctcggcgg ctccgtcgcg gtgctgaagg cgcggaacgc cggcggcgcg tgggtcgacg
     1861 gctccgtcgg caagtgcttc gcgtacggcg cgcagcgcgg ccgcttcttc gtgcagggtg
     1921 gcgccgacgc gcgcgccggc atccgcctct ccggcgccga cgtcgtcttc ggcggcgacc
     1981 tgcgcgggtt cgcgttcgag tacatgaccg gcggacgcgc cgtcgtgctc ggcgaccccg
     2041 gacggtggat ctgctcgggc atgagcggcg gcgtcgtgtt cctgcggcac gatccggcgc
     2101 gcggcatgga cgagcgcgcg ctccgcgatc gcttcgccaa gggcgccaag gtctcgctgc
     2161 gcgtgcccga cgagggcgac gccgccgcgc tcggcgagct cctcggggcg tacgcgaccg
     2221 cgctgcgcga cagcggacag gacaacgcct cgcgc
//
LOCUS       DASTZR010000311         4205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1244895, whole genome shotgun sequence.
ACCESSION   DASTZR010000311 DASTZR010000000
VERSION     DASTZR010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244895"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VFV20_06940"
     CDS             <1..803
                     /locus_tag="VFV20_06940"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903443.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEU5288119.1"
                     /translation="PTGAMFDWDTTSTYVQEPPFFKDLGPAHEIADITAARVLAKLGD
                     SVTTDHISPAGSFPPASPAGEYLMSLGVKPVDFNQYGTRRGNHEVLIRGTFANIRLRN
                     QLVQREGWWTKHIPSGEEMTIFDASVRYQKERTPLIIIAGKEYGTGSSRDWAAKGPLL
                     LGVRAVLAETFERIHRSNLVGMGILPLQFQAGESAATLGLTGEESFTVRGLASLAPKA
                     TLDVEAKRPNGETVRFKVLARVDDPVDVDYLRHGGVLPLVFRELLAKS"
     gene            complement(823..1545)
                     /locus_tag="VFV20_06945"
     CDS             complement(823..1545)
                     /locus_tag="VFV20_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288120.1"
                     /translation="MIDVMSGTIGFVRLLPWRLFGRFATAFVEVALAAVVVSGSVVAG
                     HQLREWRAWYWDPATTHPWAPTPRPTETAGAAAHVSQATARAVPTPMRAAAVATPAPA
                     CRTSSGDEQLAFTNPLPRGADCVWLALRSDGTHLIVGTYGLGPSDLGIVAMPRSLPDL
                     HVYAPNVWQAPVIATVGRVGAATEDVLVFTWQGRQLVELLRTTGTQVDLAADATGWPR
                     VSVTRHDGARAYAWDGRAYTSR"
     gene            complement(1606..2478)
                     /locus_tag="VFV20_06950"
     CDS             complement(1606..2478)
                     /locus_tag="VFV20_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288121.1"
                     /translation="MRDEFPALRTAGARLPTIASEPILWAFVAGVGAGPIVSGTVRVL
                     LLALAPVLFPATEPRPEWLTPGILATAAAGLASGVVLVRAGGAAALGAFVGYELLRAL
                     AALPGRVDFCARRFPSSGDFGAPRGCDYTALVIERWPTWLALALGAVVGVWLGRGAPG
                     VNRLLRAAGVFALVLAFFSTAVTLGSPNVTTDRQIPLLALFTFGNLVAGIADGITLDE
                     DRLASAVLLAVLIMAPGVALALPLALRNAQGPEPIETTLLRWSGVVIPLAAAAVVLGA
                     RAYMREREVPGTFS"
     gene            complement(2488..3189)
                     /locus_tag="VFV20_06955"
     CDS             complement(2488..3189)
                     /locus_tag="VFV20_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288122.1"
                     /translation="MDDALQFLAERKVLTLTQDCSLPSLFAATPEEAFDETKKGFASW
                     PKTKWSWGGEIAQRDDVFETKLHRGKTLFLAPDAARAVDPLCRAALDEADAAADDRAR
                     LVRHLKSAGPSSVEDLKRELGLDATVLRKVREGLEKSGAIIARGITTQDTKGGLRHSS
                     VLSRWDQVWRKPWKATEDTALDEIIALGVAAAVVTHEDEVRTWFTWPVERPTINALVA
                     SGKLARPASGWLAAP"
     gene            complement(3252..>4205)
                     /locus_tag="VFV20_06960"
     CDS             complement(3252..>4205)
                     /locus_tag="VFV20_06960"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEU5288123.1"
                     /translation="ELAILAELTPEFHTLLDLQQLLDRILQILQRNIPGASFTILLRE
                     EKTDDLVVRAAAGMWTDIASPNRVHEGRGISGWVLEHREAQNIEDVRLDPRYVGDPSV
                     RSEMIVPLVSGGNAIGVLVLSHRSVAAFSQRELMLMQTVGAQIAAAIDVAELHERLKR
                     AANTDALTGLHNYRYFYDRLEEEIARAERRQAPLAVAFFDLDKLKTVNDTYGHLAGNE
                     VLRLLGQTISTHVRTEDVPARYGGDEFAIVMPDTPRDEAEKVVLRLMEILEHTTVTLP
                     SRSIPMPELSWGVATYPLDGRTARELVDNADTRAYARKRSR"
BASE COUNT          630 a         1482 c         1518 g          575 t
ORIGIN      
        1 cgccgacagg ggcgatgttc gactgggaca cgacgtccac gtacgtgcag gaaccgccgt
       61 tcttcaagga cctggggccc gcgcacgaga tcgccgacat caccgccgcg cgcgtgctcg
      121 cgaagctcgg ggactccgtg acgacggacc acatctcgcc cgccgggtcc ttcccgccgg
      181 cgagcccggc cggcgagtac ctgatgtcgc tcggcgtgaa gccggtcgac ttcaaccagt
      241 acggcacgcg tcgcgggaac cacgaggtcc tcatccgcgg caccttcgcg aacatccgtc
      301 tgcgcaatca gctcgtgcag cgcgagggct ggtggaccaa gcacatcccg tccggcgagg
      361 agatgacgat cttcgacgcc tccgtccgct accagaagga gcgcacgccg ctcatcatca
      421 tcgcgggcaa ggagtacggg acgggcagct cgcgcgactg ggcggcgaag gggccgctgc
      481 tgctcggcgt gcgcgcggtg ctcgccgaga ccttcgagcg catccaccgc tccaacctcg
      541 tcggcatggg catcctcccg ctccagttcc aggcgggcga gagcgccgcg acgctgggac
      601 tcaccggcga ggagtccttc accgtccgcg ggctcgccag tctcgcgccg aaggccacgc
      661 tcgacgtcga ggcgaagcgc ccgaacggcg agacggtgcg gttcaaggtg ctcgcgcgcg
      721 tggacgaccc ggtggacgtc gactacctcc gccacggcgg cgtgctgccg ctcgtgttcc
      781 gggagctgct cgcgaagagc tgaccgagag ctcgcgcgcg cttcagcggc tggtgtaggc
      841 gcggccgtcc caggcgtacg ccctggcgcc atcgtggcgc gtgacgctga cgcgcggcca
      901 tcccgtcgcg tccgcggcga ggtccacctg cgtcccggtc gtgcgcagca gctcgacgag
      961 ttgccggcct tgccaggtga agacgaggac gtcctcggtg gcggccccga cccgcccgac
     1021 cgtggcgatg accggcgcct gccacacgtt cggtgcgtag acgtgcagat ccggcagtga
     1081 ccggggcatc gcaacgatcc cgagatcgct cggcccgagc ccgtacgtgc cgacgatgag
     1141 gtgggtaccg tccgaccgca gcgcgagcca cacgcaatcg gcgccgcgcg gcagcggatt
     1201 cgtgaacgcg agctgctcgt cgccgctcga cgtccggcac gcgggcgccg gcgtcgcgac
     1261 ggcggcggct cgcatcggcg tggggaccgc tcgcgcggtc gcctgggaca catgcgcggc
     1321 ggcgcccgcc gtctcggtcg ggcgaggcgt cggcgcccac gggtgcgtcg tggccggatc
     1381 ccagtaccac gcccgccact cgcgcagctg gtggccggcg acgacggatc cggagaccac
     1441 gaccgcggcg agcgcgacct cgacgaacgc cgtcgcaaaa cggccgaaca gcctccacgg
     1501 cagcaggcgc acgaagccga tcgtgccgga catgacgtcg atcactctcc gctcgtgatg
     1561 aagtgcgcgc gtccgcgctg gcggcgcgca actactacgt ccgcgctacg agaaggttcc
     1621 cggcacctcg cgctcacgca tgtacgcgcg cgccccgagg acgaccgccg cggcggcgag
     1681 cggaatgacg acgcccgacc agcggaggag ggtcgtctcg atcggctccg gtccctgggc
     1741 gttgcgcagg gcgagcggga gggcgagcgc gactccgggc gccatgatga gcaccgcgag
     1801 cagcaccgcc gacgcgagcc gatcctcgtc gagcgtgatc ccgtccgcga tgccggcgac
     1861 gaggttgccg aaggtgaaga gggcgagcag cgggatctgg cggtccgtcg tgacgttcgg
     1921 ggatcccagc gtgacggcgg tgctgaagaa cgccagcacc agcgcgaaga cgccggcggc
     1981 gcggagcagg cggttcacgc cgggcgcgcc acggcccaac cacacgccca ccacggcacc
     2041 gagcgccagc gcgagccatg tcggccaccg ctcgatcacg agcgccgtgt aatcgcagcc
     2101 acggggcgcg ccgaagtcgc cgctcgatgg gaaccggcgc gcgcagaagt ccacgcgccc
     2161 gggaagcgcc gcgagcgcgc ggaggagctc gtatccgacg aacgcgccga gcgcggccgc
     2221 gccacccgcg cgcaccagca cgaccccgct cgcgaggccg gccgcggcgg tcgcgaggat
     2281 gccgggcgtg agccattccg gacgcggctc ggtcgcgggg aacagcaccg gcgcgagcgc
     2341 cagcaagagc acgcgcaccg tgccgctcac gatcgggccg gcaccgacgc cggcgacgaa
     2401 cgcccagagg atcggctccg acgcgatcgt cggcagacgt gcgccggccg tacgcaaagc
     2461 ggggaactcg tcgcgcatca gcgaaggcta cggcgcggcg agccaccccg acgccggccg
     2521 cgccagcttc ccggacgcga cgagggcgtt gatggtcggc cgctcgaccg gccaggtgaa
     2581 ccaggtgcgg acctcgtcct cgtgcgtgac gacggcggcg gcgacgccga gcgcgatgat
     2641 ctcgtcgagc gcggtgtcct cggtcgcctt ccacggcttg cgccagacct gatcccagcg
     2701 cgacaggacg ctcgaatggc gaagcccgcc tttggtgtcc tgggtggtga tgccgcgggc
     2761 gatgatcgcg ccgctcttct ccagcccctc gcgcaccttg cgcagcacgg tggcgtcgag
     2821 gccgagctcg cgtttgaggt cttccacgct gctcgggccg gccgacttca ggtgccgcac
     2881 gagacgcgcg cgatcgtcgg ccgcggcatc cgcctcgtcg agcgccgcgc ggcacagcgg
     2941 atcgaccgcg cgggccgcgt cgggtgcgag gaacagcgtc ttgccgcggt gcagcttggt
     3001 ctcgaagacg tcgtcgcgct gcgcgatctc gccgccccag ctccacttcg tcttcggcca
     3061 cgaggcgaag cccttcttcg tctcgtcgaa cgcctcctca ggggtggcgg cgaagaggct
     3121 gggaaggctg cagtcctgcg tgagggtcag caccttgcgc tcggcgagga actggagggc
     3181 atcgtccaat gtccgcagct tccggtcggg agtgagcctg cagtcgtgga gccgccgagc
     3241 gcggagctcc gctagcggga tcgcttcctc gcgtaggccc gggtgtcggc gttgtcgacg
     3301 agctcccgcg cggtgcgccc gtccagtgga tacgtcgcga cgccccacga aagctcgggc
     3361 atcggaatgc tgcggctcgg gagcgtcacc gtcgtgtgct cgaggatctc catcagtcgc
     3421 agcacgacct tctccgcctc gtcgcgcggc gtatcgggca tgacgatggc gaattcgtcg
     3481 ccgccgtagc gcgccgggac gtcctcggtg cggacatggg tcgagatggt ctgaccgagc
     3541 aggcgcaaga cctcgttccc ggcgaggtgc ccgtatgtgt cgttcaccgt cttcagctta
     3601 tcgaggtcga agaatgccac agccagcggc gcctggcggc gctcggcccg ggcgatctcc
     3661 tcctccaggc ggtcgtagaa gtagcggtag ttgtggagcc cggtcagcgc gtcggtgttg
     3721 gcggcgcgct tcaggcgctc gtgcagctcg gcgacgtcga tggcggcggc gatctgcgcg
     3781 ccgacggtct gcatgagcat cagctcgcgc tgtgagaacg cggccacgct gcggtgcgag
     3841 agcacgagga cgccaatagc gttcccgccg gaaacgagcg gcacgatcat ctccgaccgg
     3901 acgctcgggt cgccgacgta gcgcggatcg aggcgcacgt cctcgatgtt ctgcgcttcg
     3961 cggtgctcca gcacccatcc ggagatccca cgaccttcgt gcacccggtt cggcgacgcg
     4021 atgtcggtcc acatgccggc agccgcacgc acgaccagat cgtcggtctt ctcctcgcgc
     4081 aggaggatgg tgaagctcgc gccggggatg ttgcgctgca ggatctgcag gatgcggtcg
     4141 agcagctgct gcaggtcgag gagcgtgtgg aactcgggcg tgagctccgc gaggatggcg
     4201 agctc
//
LOCUS       DASTZR010000312         2757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_174288, whole genome shotgun sequence.
ACCESSION   DASTZR010000312 DASTZR010000000
VERSION     DASTZR010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174288"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..804
                     /locus_tag="VFV20_06965"
     CDS             115..804
                     /locus_tag="VFV20_06965"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HEU5288124.1"
                     /translation="MNIAIAFGAGILSFVSPCVLPLVPAFLVNMAGEAAISGTQRMRT
                     LAHALAFVLGFSVVFTLLWVLVAIVGALAGELVYWLQRLGGVLLIVLGMHMLGLITIP
                     LLARELDLRLERNTTGLPRSFLIGSAFGIGMTPCVGPYLAVILNLLLNDDLASGAFLL
                     LAYSLGLGVPFLVTAAWVGGARRVTDALRRWIRVLNVIGGIFVILMGLLLLSGQFARL
                     PQLFNFLPLLG"
     gene            804..1397
                     /locus_tag="VFV20_06970"
     CDS             804..1397
                     /locus_tag="VFV20_06970"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU5288125.1"
                     /translation="MDTAGDLRPKMPRAIAALVIGAIALASLAALVWPQLPVANLFTG
                     PTEVGVVRRVIADPNAAPRISGVAPDFEWNAPSGKTQKLSDLRGRVVVLTFWATWCEP
                     CRREFPAMQRVAASTDAVFIAVDLLEDGSKVRSFMDALALDRIDPLLDTDGALTRAYA
                     AIELPQTFFIDARGVIQHIERGELDEAGVRRGIDKAR"
     gene            1398..1850
                     /locus_tag="VFV20_06975"
     CDS             1398..1850
                     /locus_tag="VFV20_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288126.1"
                     /translation="MPPRQPDAEVMRVLRDTKVLVAEGQIALAIDLFEETWADLVSRG
                     QHFSASHVAHMAGAVDPVPERKLQWNERAIAAADAEPDRPGIEWFYPSLYNNLAYSLV
                     LLGRRDEALRAERTAWSRIETLEPGPYADQVRSVIQRRLAELIGEAKD"
     gene            1884..2261
                     /locus_tag="VFV20_06980"
     CDS             1884..2261
                     /locus_tag="VFV20_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288127.1"
                     /translation="MATEFIVKLADRPGQLAALGSALGDANVQLRGIAAQKGVVGLLV
                     DDRDRAKARRALKKARYRTTERSAIEMRLQDKPGSLGKAAGRFGKNKVNISSMYVLAP
                     GRGNVRVAFGVKDARGAKRALGR"
     gene            complement(2265..2723)
                     /locus_tag="VFV20_06985"
     CDS             complement(2265..2723)
                     /locus_tag="VFV20_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288128.1"
                     /translation="MDSAPPEPEPDPRRASRRRAALVSILQQRADAVAERVVADALRA
                     SDFMGLGQSVATRFLGTAQRSMPEALNALGADDPDRSRLLESFARHVHELLTLGVPKF
                     VQRALVAAGFAIAQGIARSGARAHGFEPNELEDELRTFQRAMEERLFFGA"
BASE COUNT          373 a          970 c          985 g          429 t
ORIGIN      
        1 gtacggcagc atgcgaccag tgtcgagcca tgcgtcgccc accgcgtcgg gcgccatccg
       61 ctccgcgcgt gcggtggggc catacgcgcc ggacgacgac ctaccctgta agcggtgaac
      121 atcgccatcg cgttcggcgc cgggatcctc tccttcgtct cgccgtgcgt gctcccgctc
      181 gtccccgcct tccttgtgaa catggcgggc gaggccgcga tctcggggac gcagcgcatg
      241 cggacgctcg cgcacgcgct cgcgttcgtg ctcggcttca gcgtggtgtt cacgctgctc
      301 tgggtgctcg tggccatcgt cggtgcgctc gcgggagagc tcgtgtactg gctgcagcgc
      361 ctcggcggcg tgctgctcat cgtgctcggc atgcacatgc tcggtctcat cacgatcccg
      421 ctgcttgcgc gcgagctgga cctgcgtctc gagcgcaaca ccaccggcct cccgcgctcg
      481 ttcctcatcg gatccgcatt cggcatcggc atgacgccgt gcgtcggccc gtatctcgcg
      541 gtcatcctca acctgctcct gaacgacgac ctcgcctcgg gcgcgttcct gctgctggcg
      601 tactcgctcg gcctgggagt gccgttcctc gtgacggcgg cgtgggtcgg cggcgcgcgg
      661 cgcgtgaccg atgcgctgcg gcgctggatc cgggtgctga acgtgatcgg cggtatcttc
      721 gtcatcctga tgggactgct ccttctgtcc gggcagttcg cccggctgcc gcagctcttc
      781 aacttcctgc cgctcctcgg ttgatggaca cggccggcga cctccgaccg aagatgccgc
      841 gggccatcgc ggcgctggtc atcggcgcga tcgcgctcgc ctcgctcgcc gccctcgtct
      901 ggccgcagct cccggtcgcg aatctcttca ccgggccgac cgaggtcggc gtcgtgcggc
      961 gcgtgatcgc ggacccgaac gccgcgccgc gcatcagcgg cgtcgcgccg gacttcgagt
     1021 ggaacgcccc ctccgggaag acgcagaagc tgtcggatct tcgcgggcgc gtggtcgtcc
     1081 tcacgttctg ggccacctgg tgcgagccgt gccgccgcga gttcccggcg atgcagcgcg
     1141 tcgccgcgtc gaccgacgcg gtgttcatcg cggtcgatct cctcgaggac ggttcgaagg
     1201 tccgctcgtt catggacgcg ctcgcgctcg accgcatcga cccgctgctc gacacggatg
     1261 gagcgctcac gcgcgcgtac gccgcgatcg agctcccgca gacgttcttc atcgacgcgc
     1321 gcggcgtcat ccagcacatc gagcgcggcg agctcgacga ggccggcgtg cgccgcggga
     1381 tcgacaaggc gcgctaggtg ccgccgcggc agccggacgc cgaggtgatg cgcgtgctgc
     1441 gcgacaccaa ggtgctcgtc gccgaggggc agatcgcgct cgcgatcgac ctcttcgagg
     1501 agacctgggc ggacctcgtc tcgcgggggc agcacttcag cgcgagccac gtggcgcaca
     1561 tggccggcgc ggtcgacccg gtccccgagc ggaagctcca gtggaacgag cgagcgatcg
     1621 ccgccgccga cgccgagccg gaccgcccgg gtatcgagtg gttctacccc tcgctgtaca
     1681 acaacctggc gtactcgctc gtcctcctcg gacgccggga cgaggcgctt cgcgcggaac
     1741 gcacggcctg gtcgcgcatc gagacgctgg aaccgggccc gtacgccgat caggtgcgga
     1801 gcgtgatcca gcggcgactc gccgagctca tcggcgaggc gaaggactag catcgcgcgc
     1861 atctacgaac ggggaggcgt gacatggcga ccgagttcat cgtcaagctc gcggatcggc
     1921 cgggccagct cgcggcgctc ggatcggcgc tcggtgacgc gaatgtgcag ctgcgcggca
     1981 tcgcggcgca aaagggcgtc gtcggcctgc tcgtcgacga tcgtgatcgc gcgaaggcgc
     2041 ggcgcgcgct gaagaaagcc cgctaccgca cgaccgagcg cagcgcgatc gagatgcgcc
     2101 tgcaggacaa gccggggtcg ctcggcaagg ccgccggccg cttcggcaag aacaaggtga
     2161 acatctcgtc gatgtacgtc ctcgcgccgg gccgcggcaa cgtgcgcgtg gcgttcgggg
     2221 tgaaggacgc ccgcggggcg aagcgcgcgc tcgggcggta agccctaggc gccgaagaag
     2281 agccgctcct ccatcgcgcg ttggaaggtg cgcagctcgt cctcgagctc gttcggctcg
     2341 aagccgtgtg cgcgcgcgcc ggaccgcgcg atgccctgcg cgatggcgaa gccggctgcg
     2401 acgagcgcgc gctgcacgaa cttcgggacc cccagggtga gcagctcgtg cacgtggcgg
     2461 gcgaagctct cgaggagacg cgaccggtcc gggtcgtccg cgccgagcgc attcagggcc
     2521 tcgggcatcg agcgctgcgc ggtgccaaga aagcgcgtgg ccacggattg cccgagaccc
     2581 atgaagtcgc tcgcgcgtag cgcgtcggcg acgacgcgct cggccactgc atcggcgcgc
     2641 tgctggagga tcgagacgag cgccgcgcgg cgccggctgg cgcggcgcgg atcgggttcg
     2701 ggctccggtg gcgccgagtc catctagctc gcgactgtaa gacgcgggac tggatcg
//
LOCUS       DASTZR010000313         2655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_696343, whole genome shotgun sequence.
ACCESSION   DASTZR010000313 DASTZR010000000
VERSION     DASTZR010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696343"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VFV20_06990"
     CDS             complement(<1..734)
                     /locus_tag="VFV20_06990"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU5288129.1"
                     /translation="MGVAATHLPSGRHLAVREDELFPLASLVKVPILITLYREVHAGR
                     IDLRERVAYQSATRVPGSGVLQDLDDGLSVTVRDLATLMVTVSDNTAADVLLARLGKD
                     RVEQAMHDLGAVSVRVPFSIRELFEEITDAPGADYDTLSERLRLSSGSGGRAVIAEQT
                     VRASPRDICSLFERLERRTVVDAPSCDAMLDILKRTKTDTRIPALLPKGTVVAHKTGT
                     IRGVRCDAGVIYAPGGPYALAILSKGV"
     gene            complement(788..2569)
                     /gene="aspS"
                     /locus_tag="VFV20_06995"
     CDS             complement(788..2569)
                     /gene="aspS"
                     /locus_tag="VFV20_06995"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEU5288130.1"
                     /translation="MLRTKTCGELRATDAGTEVTLAGWVHTRRDHGDVTFIDLRDRYG
                     LTQIVAHGKESAAAHAALKDARSEWVLQVKGAVRARPEGTRNPKLATGDVEVLASEIT
                     VLNESKTPPFYVNEETPVDENLRWKYRYVDLRRARSRDLIGLRHEVVAYIRRFMTERG
                     FWEVETTDLIRSDPTGARDLIVPSRYYPGRFWALPQSPQQLKQILMVGGIDRYFQIAR
                     CFRDEDPRADRTLEHSQLDIEMSFVEPKDVMALGEELYTSIFQKFATKPLEQVPWPRI
                     TFAEAMRRFGSDRPDTRFGMELIDLTDAFRATSFNAFRGAIDGGGAVRAMVVPGKADA
                     TRKEVDGWVQVAKRKGAKGLAQFAFKGSEIASQVAKFLTPAELDAIRRATGAKDGDLV
                     LAVADSYDVASESLGVVREHLGASLRLADTSTHHALWVHEFPLVQRTPDGGWTFAHNP
                     FCGPLTMADLELLDTEPEKARSKQYDLVVDGRELGGGSVRVHQRWVQERIFKQMGISK
                     EEAQTRFGALLDALEYGAPPHGGIAPGIDRLVMTLAGTENIREVQAFPKTQTGYDPLL
                     DAPAPVDAKLLEELGIRVVAPSKEARD"
BASE COUNT          369 a          945 c          910 g          431 t
ORIGIN      
        1 acgcccttcg agaggatcgc gagcgcatac gggcccccgg gcgcgtagat gaccccggcg
       61 tcgcaccgca cgccgcggat cgtgccggtc ttgtgcgcca ccaccgttcc tttcggcagc
      121 agcgcgggaa tccgcgtgtc cgtcttcgtg cgcttcagga tgtcgagcat ggcgtcgcac
      181 gagggcgcat ccacgaccgt gcgccgctcg aggcgctcga agagcgagca gatgtcgcgc
      241 ggactcgcgc gcacggtctg ctccgcgatg acggcccggc cgccgctgcc ggacgagagc
      301 cggagccgct cgctcagcgt gtcgtagtcc gcaccaggag cgtcggtgat ctcctcgaag
      361 agctcgcgga tcgagaacgg cacgcggacg ctcaccgcgc cgaggtcgtg catcgcctgc
      421 tcgacgcggt cctttcccag gcgcgcgagg agcacgtccg ccgcggtgtt gtccgagacc
      481 gtgaccatga gggtcgcgag gtcgcgcacc gtcacgctga gcccgtcatc gaggtcctgc
      541 agcacacccg agcccggtac gcgtgtcgcg ctctggtagg ccacgcgttc ccgcaggtcg
      601 atgcgacctg cgtggacctc gcgatacagc gtgatgagga tcggcacctt cacgagcgac
      661 gcgagcggga agagctcatc ctcgcgcacc gccaggtgac gaccgctcgg gaggtgcgtg
      721 gccgcgacgc ccacggtgcc gccggtcttc gcgatgagcg gctcgatctt ccggacgagc
      781 gcgtcggtca gtcgcgcgcc tccttcgacg gtgcgacgac gcgtatcccc agctcctcga
      841 ggagcttcgc gtcgacgggg gctggcgcgt cgagcagggg gtcgtaaccg gtctgcgtct
      901 tcgggaaggc ctgcacctcc cggatgttct cggtgccggc gagcgtcatg acgaggcggt
      961 cgatgcccgg cgcgatgccg ccgtgcggcg gcgcgccgta ctcgagcgcg tcgagcagcg
     1021 caccgaaccg ggtctgcgct tcctccttcg agatccccat ctgcttgaag atgcgctcct
     1081 gcacccagcg ctggtgcacg cgcacgctgc cgccgcccag ctcgcggccg tcgacgacga
     1141 ggtcgtactg cttgctccgc gccttctccg gctcggtgtc gagcagctcg aggtccgcca
     1201 tcgtgagcgg accgcagaac gggttgtgcg cgaacgtcca gccgccatcg ggcgtgcgct
     1261 gcaccagcgg gaactcgtgg acccagaggg cgtggtgcgt ggacgtatcc gccagccgca
     1321 gtgaagcgcc caggtgctcg cgcacgaccc cgagcgactc gctcgcgacg tcgtagctat
     1381 cggccaccgc gaggacgaga tccccgtcct tcgcgccggt cgcccgacgg atcgcatcga
     1441 gctcggccgg cgtgaggaac ttggcgacct gggacgcgat ctcgctgccc ttgaaggcga
     1501 actgggcgag ccccttcgcg cccttgcgct tcgcgacctg gacccagccg tccacctcct
     1561 tgcgcgtcgc gtccgccttg ccgggcacga ccatcgcgcg caccgcgccg ccgccgtcga
     1621 tcgcgccacg gaacgcgttg aacgacgtcg cgcggaacgc gtcggtgagg tcgatgagct
     1681 ccatcccgaa acgcgtgtcg ggccggtcgc tgccgaagcg ccgcatggcc tccgcgaacg
     1741 tgatgcgcgg ccacggcacc tgctccagcg gcttcgtcgc gaacttctgg aagatgctcg
     1801 tgtacagctc ctcgccgagc gccatgacgt ccttcggctc gacgaagctc atctcgatgt
     1861 ccagctggct gtgttccagc gtccggtcgg cccgcgggtc ctcatcgcgg aagcagcgtg
     1921 cgatctggaa gtacctatcg atcccgccca ccatcaggat ctgcttcagc tgctggggcg
     1981 actgcggcag cgcccagaac ctgcccgggt agtagcgcga cgggacgatg aggtcgcgcg
     2041 cgccggtcgg gtccgagcgg atgagatcgg tggtctcgac ctcccagaat ccgcgctcgg
     2101 tcatgaagcg gcggatgtac gcgacgacct cgtgccggag gccgatgagg tcacgagagc
     2161 gcgcgcgccg cagatcgacg tagcgatact tccagcgcag gttctcatcg acgggcgtct
     2221 cctcgttgac gtagaacggc ggcgtcttcg attcgttcag cacggtgatc tcgctcgcga
     2281 ggacctcgac gtcgccggtg gcgagcttcg ggttgcgcgt gccctcgggg cgggcgcgca
     2341 ccgctccctt cacctggagc acccactcgc tgcgcgcgtc cttcagcgcg gcgtgcgcgg
     2401 cggcgctctc cttgccgtgc gcgacgatct gcgtgagccc gtagcggtcg cgcaggtcga
     2461 tgaacgtcac gtcgccgtgg tcgcggcgcg tgtgcaccca gccggcgagg gtgacctcag
     2521 tccccgcgtc ggttgcgcgc aactctccac aggtcttcgt acggagcatc tagtcccctt
     2581 ccacgccggg catgtcagtc cctcgggtcc gtgaggtacg gtgcctcacc cgcggcaccg
     2641 ggctcctggt caccg
//
LOCUS       DASTZR010000314         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_226451, whole genome shotgun sequence.
ACCESSION   DASTZR010000314 DASTZR010000000
VERSION     DASTZR010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226451"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VFV20_07000"
     CDS             complement(<1..321)
                     /locus_tag="VFV20_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288131.1"
                     /translation="MESGIYVTFFDAGEPPERELPPVGPLEHVVLRHRELVAERRTVG
                     QAPDTGVEIARWLEAELELQRATGEEPGGPRRAEMRILARDGVYVRFAVFGDVRERDS
                     VAELG"
     gene            complement(378..1157)
                     /locus_tag="VFV20_07005"
     CDS             complement(378..1157)
                     /locus_tag="VFV20_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU5288132.1"
                     /translation="MTVTSPPTVHVRTEDGVATVLLDRPPLNVLTIAMLNELESALRD
                     LGAVPDLRLIVIRGAGRVFSAGVDVGEHVGDALEPMLDAFARVSRRLLETPVPTLAVI
                     HGAALGGACEIAAVCDLAVTTDDAKLGTPEIALGVVPPVGAAMFPLLVGPQRARALVL
                     LGEPVRGTEAAAIGLVWKSVAPDALDGEVTRIVERFRSLSGASLRLAKRTFRVAELAR
                     TPVEAVMAADAEQRRSLPRMHDANEGLRAFLEKRAPRWLHR"
     gene            complement(1154..>2030)
                     /locus_tag="VFV20_07010"
     CDS             complement(1154..>2030)
                     /locus_tag="VFV20_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEU5288133.1"
                     /translation="PTFKTPPLVLGHEISGTVVRVGAGVDAARIGTSVLLAPVTSCGE
                     CAMCRTGRENVCTRQKMLGNTIDGGFAEFVVAPSRDAFEIPPDVPAEEACVIADALTT
                     AFHAVTRRARLETGENVVVVGCGGLGLNVVQVASLVGARVIAVDIDPRKLALAKELGA
                     SLTIDAREGDAAKRVRAETSGGADCAIEAIGNPKTQEQALASLRTGGRLVLLGFAAEN
                     MSLPGGRVTYREITVIGTLGCRPIDFPIVLDLVRRGRLALGPLVTHRHPLDRINEGFD
                     ALRRGEGVRHIAVIS"
BASE COUNT          289 a          733 c          748 g          260 t
ORIGIN      
        1 gcccagctcg gcgacgctgt cccgctcgcg cacgtcaccg aagaccgcga agcgcacgta
       61 cacgccatcg cgtgcgagga tccgcatctc cgcgcgcctc gggccgcccg gctcttcgcc
      121 ggtggcgcgc tgcagctcga gttcggcctc gagccaccga gcgatctcga cgccggtatc
      181 gggcgcctgg ccgacggtgc ggcgctcggc cacgagctcg cggtggcgca ggacgacgtg
      241 ctcgagcgga ccgacgggcg gcagctcgcg ctcgggcggc tcgcctgcgt cgaagaacgt
      301 cacgtagatg ccggattcca ctgcggcgga agcctaacgt cgcggcccgt cgctgagtac
      361 ccgcggcgga gggaggtcta gcggtgcagc cagcggggcg cgcgcttctc gaggaaggcg
      421 cgaaggccct cgttcgcgtc gtgcatccgc gggagcgagc gccgctgctc cgcgtccgcc
      481 gccatcacgg cctcgacggg agtgcgtgcc agctcggcca cgcggaacgt gcgcttcgcg
      541 aggcgcaggc tcgcgcccga aagcgaccgg aagcgctcga cgatcctggt gacctcgccg
      601 tcgagcgcat cgggcgcgac gctcttccac accaggccga tcgccgccgc ctcggtgccg
      661 cggaccggtt cgccgaggag cacgagcgcg cgggcgcgct gcggtccgac gagcagcggg
      721 aacatcgccg caccgacggg cggcacgacg ccgagcgcga tctccggagt gccgagcttc
      781 gcgtcgtcgg tcgtgacggc gaggtcgcag acggccgcga tctcgcacgc tccgccgagc
      841 gccgcgccgt ggatcaccgc gagcgtcggc accggggtct cgaggagtcg ccgcgacacg
      901 cgcgcgaagg cgtcgagcat cggctcgagc gcgtcgccga cgtgctcgcc gacgtcgacc
      961 ccggcggaga agacgcgacc cgcgccgcgg atcacgatga gccggaggtc cggcaccgct
     1021 ccgagatcgc gcagcgcgct ctcgagctcg ttcagcatcg cgatcgtgag cacgttgagc
     1081 ggggggcggt cgagcaggac cgtcgcgaca ccgtcctcgg tccgtacgtg aacggtcgga
     1141 gggctggtga ccgtcacgag atcaccgcga tgtggcgcac gccctcgccg cgccgcaggg
     1201 cgtcgaagcc ctcgttgatc cggtccagtg ggtgacggtg cgtcacgagc ggtcccagcg
     1261 cgagcctgcc gcggcgcacg agatcgagga cgatcgggaa gtcgatgggc cggcaaccca
     1321 gcgtgccgat gaccgtgatc tcgcggtacg tcacgcgccc gccggggagt gacatgttct
     1381 cagcggcgaa gccgagcagg acgagacggc cgccggtccg cagcgacgcg agcgcctgct
     1441 cctgcgtctt ggggttgccg atcgcctcga tggcgcagtc cgcgccaccg gaggtctccg
     1501 cacgcacgcg cttggccgcg tcaccctcgc gcgcgtcgat cgtgagcgat gcgccgagct
     1561 ccttcgcgag cgcgagcttt cggggatcga tgtcgacggc gatcacgcgg gccccgacga
     1621 gcgaggcgac ctgcacgaca ttgagcccga ggccgccgca gccgaccacg accacgttct
     1681 cgcctgtctc cagcctcgcg cgccgggtca cggcgtggaa cgccgtggtg agggcatcgg
     1741 cgatgacgca ggcctcctcc gcgggcacgt cgggcgggat ctcgaacgcg tcgcgcgacg
     1801 gcgcgacgac gaactcggcg aagccaccgt cgatggtgtt gccgagcatc ttctggcgcg
     1861 tgcagacgtt ctcacggccg gtccggcaca tggcgcactc gccgcacgag gtcaccggcg
     1921 ccagcagcac gctcgtgccg atgcgcgcgg cgtcgacgcc ggcgcccacg cgtaccacgg
     1981 tgccggagat ctcgtggccg aggacgagcg gcggcgtctt gaacgtcggc
//
LOCUS       DASTZR010000315         3358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_174535, whole genome shotgun sequence.
ACCESSION   DASTZR010000315 DASTZR010000000
VERSION     DASTZR010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174535"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VFV20_07015"
     CDS             <1..219
                     /locus_tag="VFV20_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288134.1"
                     /translation="EGMTAFAEKRKADRLGVRARARDGAPSETLWGPYAKTCPSCGTK
                     GIPEGFAYCGVCGKALAAAPEPVGARRG"
     gene            212..970
                     /gene="fabG"
                     /locus_tag="VFV20_07020"
     CDS             212..970
                     /gene="fabG"
                     /locus_tag="VFV20_07020"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEU5288135.1"
                     /translation="MAKEGRDLEGAVALVTGGSRGIGRAISVDLARHGCDVAFTYRSD
                     NDGAKKTAHDIEATGKCALGLKADGRDPAAPGKAVMDVVGRFGRLDILVNNAGLNRDG
                     VVWKMTDDAWNEVIDADLGAAFRFTRAVIPIFREAGVGRIVNISSINGLRGKFGQSNY
                     AAAKAGMIGFTKSIARELGSFNITANVVAPGLIATDMVKGMPDAAKEKSLAEIVLGKL
                     GTPEDVAAAVTFLAGPGGRHITGVVLQVDGGQYL"
     gene            complement(967..1422)
                     /locus_tag="VFV20_07025"
     CDS             complement(967..1422)
                     /locus_tag="VFV20_07025"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEU5288136.1"
                     /translation="MFSIHILLLIGHILFASVTVGATVSYAFWIALAELEPRHLAFTI
                     RAVRHSDRLVAIPAYLLTFVTGIWLVYESGVPLDRLWIVVSLVVYLAVLVVGFAVFGP
                     TVRRELVALERGGVNDAEYRRLRGQAQLLSLGTIAALAMILALMVVKPY"
     gene            1506..2621
                     /locus_tag="VFV20_07030"
     CDS             1506..2621
                     /locus_tag="VFV20_07030"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288137.1"
                     /translation="MRVGALLLVLIVAAACAAPPKTTTAESAAPPTSAAPVTTTAPAV
                     TGVATASPTPAATPYRSLRVFVASDMGETVTVLEGGATFAVVGQIAVGKMPHNLAVSP
                     DARWIATGNRMANTVSIIDPYAMKEVARVGVGRQPHDLTWSLDSKTLYVGHERDTIIA
                     RIEAGTWKPLTPLVVGVPQHDLALSATRPNEIFFTVTNSMESDHLRVYDLVSNQITKF
                     KVQDVHDVFFTPDSSEVWTTSSGYVGVPSDRLVASDPATRTMKAEFILKGRYPFHTMK
                     PGRDGMFYPPAGTPMLLTDHNGPSLLWMDAKTRTIVGETKVGPEPYHSSYDPIGDRVL
                     VTSNKDGNVWVIDRKTHAVLQTVHVHGAHGMVAVGLP"
     gene            2693..3127
                     /locus_tag="VFV20_07035"
     CDS             2693..3127
                     /locus_tag="VFV20_07035"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEU5288138.1"
                     /translation="MRITDRFSSEHDVFLAQIEVLASLASSAASVEAVSSAMRTLAKP
                     LLAHAEAEERALFPGLEASMGGEGGPLAILTDEHVTIHGQVDELTGTPTRAQLDRTFA
                     AFRSLLQAHIDKEEQVLFPAAQQILGDDRLERMDRDRAGLAV"
     gene            3127..>3358
                     /locus_tag="VFV20_07040"
     CDS             3127..>3358
                     /locus_tag="VFV20_07040"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU5288139.1"
                     /translation="MSGRAPVWDPALDELATTMRAAIFTGPNAPLQLGTVARPEPAAG
                     DVLVKVTACGLCHSDLHYMDHGVPTFKTPPLVL"
BASE COUNT          570 a         1180 c         1146 g          462 t
ORIGIN      
        1 gagggcatga ccgcgttcgc ggagaagcga aaggcggacc gcctcggcgt gcgcgcgcgt
       61 gcgcgcgacg gcgcaccgag cgagacgctc tgggggccgt acgcgaagac gtgcccgtcg
      121 tgcggcacga agggcatccc agagggcttc gcgtactgcg gcgtgtgcgg gaaggcgctc
      181 gcggcggcac cggagccggt gggcgcacga cgtggctaag gagggccgcg acctcgaggg
      241 cgcggtcgcg ctggtcaccg gcgggtcccg cggcatcggt cgcgccatct ccgtcgacct
      301 cgcgcgccac gggtgcgacg tcgcgttcac ctatcgcagc gacaacgacg gcgcgaagaa
      361 gaccgcgcac gatatcgagg cgaccgggaa gtgcgcgctc ggcctgaagg ccgacgggcg
      421 cgatcccgcc gctcccggca aggccgtgat ggacgtcgtg ggccgcttcg gccggctcga
      481 catcctcgtg aacaacgcgg gcctcaaccg cgacggcgtc gtctggaaga tgaccgacga
      541 cgcgtggaac gaggtcatcg acgcggacct gggcgccgcg ttccgcttca cccgtgcggt
      601 gatcccgatc ttccgcgagg ccggcgtggg ccgcatcgtg aacatctcgt cgatcaacgg
      661 gctccgcggg aagttcggtc agtcgaacta cgcggcagcg aaggccggca tgatcggatt
      721 caccaagtcc atcgcacgcg agctgggctc gttcaacatc acggcgaacg tggtcgcacc
      781 cgggctgatc gcgaccgaca tggtgaaggg catgcccgac gccgcgaagg agaagagcct
      841 cgccgagatc gtgctcggca agctcggcac gcccgaagac gtcgccgcgg cggtcacctt
      901 cctcgcgggt cccggcggac ggcacatcac cggagtcgtc ctccaggtcg acgggggcca
      961 gtacctctag tagggcttca ccaccatgag cgcgaggatc atggcgagcg cggcgatcgt
     1021 accgagggag agcagctgcg cctgaccgcg gagccgcctg tactccgcgt cgttgacacc
     1081 gccacgctcg agcgcgacca gctcacggcg cacggtggga ccgaacacgg cgaatccgac
     1141 caccaggacc gcgaggtaca cgaccagcga cacgacgatc cagagccggt cgagcgggac
     1201 gccgctctcg tacaccagcc agatccccgt cacgaacgtc aggaggtacg cggggatcgc
     1261 gacgaggcga tcgctgtgac gaaccgcgcg gatggtgaac gccaggtgac gcggctcgag
     1321 ctcggcaagg gcgatccaga acgcgtacga gaccgtcgcg ccgacggtca ccgaggcgaa
     1381 caggatgtga ccgatgagca acaggatgtg tatcgagaac accggtcgca tcgtatcgcc
     1441 gcacgtcggt atccgtagcg tgccgtgaca cggaccggac acgatgcgcc cgtagcgtcg
     1501 cgtccgtgcg ggtgggtgcg ctcctcctcg tcctgatcgt cgccgcagcg tgtgcggcgc
     1561 cgcccaagac gacgacggcc gagagcgctg cgccgccgac gagcgccgca cccgtcacga
     1621 cgaccgcacc agcggtgacc ggggtggcga ccgcgtctcc cacgccggcc gccaccccct
     1681 accgatcgct gcgcgtgttc gtggcgtcgg acatgggcga gaccgtcacc gtgctcgagg
     1741 gcggcgcgac gttcgccgtc gtcggccaga tcgccgtcgg caagatgccg cacaacctcg
     1801 cggtctcgcc ggacgcccgc tggatcgcga cgggcaaccg catggcgaac acggtctcga
     1861 tcatcgaccc gtatgcgatg aaggaggtcg cgcgcgtcgg cgtcggacgc cagccgcacg
     1921 acctcacgtg gtcgctcgac tcgaagaccc tctacgtggg tcacgagcgt gacacgatca
     1981 tcgcgcgcat cgaagcgggc acctggaagc cgctcacgcc gctcgtcgtc ggcgtcccgc
     2041 agcacgacct ggcgctctcc gcgacgcggc ccaacgagat cttcttcacg gtcacgaact
     2101 cgatggaatc ggaccacctg cgcgtctacg acctcgtgtc caaccagatc acgaagttca
     2161 aggtccagga cgtccacgac gtcttcttca ccccggactc gagcgaggtc tggaccacgt
     2221 cgtcgggcta cgtcggcgta ccgtccgatc ggctcgtcgc atccgatccg gcgacgcgga
     2281 cgatgaaggc cgagttcatc ctgaagggcc gttatccgtt ccacaccatg aagccgggtc
     2341 gggacggcat gttctatccg ccggcgggca cgccgatgct gctcaccgat cacaacgggc
     2401 cgagcctcct ttggatggac gcgaagacgc gcacgatcgt cggcgagacg aaggtcggtc
     2461 ccgagccgta ccactcgagc tacgacccga tcggcgaccg cgtgctcgtg accagcaaca
     2521 aggacggcaa cgtctgggtc atcgaccgga agacccacgc cgtcctgcag acggtgcacg
     2581 tccacggcgc ccacggcatg gtggcggtcg ggctgccctg acccgggctg tcaccgcctg
     2641 acacaggacg gacatggtcg gccgatacgt tcgccgcaag gaggtggccc ccatgcggat
     2701 caccgatcgc ttctcgagcg agcacgatgt cttcctcgct cagatcgagg tcctggcgtc
     2761 cctggccagc tcggccgcga gcgtggaggc cgtcagctcc gcgatgcgca cccttgcgaa
     2821 gccgctcctg gcgcacgcgg aggccgagga gcgggcactc ttcccgggac tcgaggcgtc
     2881 catgggcggc gagggcggtc cgctcgcgat cctcaccgat gagcacgtga ccatccacgg
     2941 ccaagtcgat gagctcacgg gtacaccgac gcgcgcccag ctcgaccgca cgttcgccgc
     3001 gttccgctcg ctcctgcaag cgcacatcga caaggaggag caggtgctct tccccgccgc
     3061 gcagcagatc ctcggtgacg accggctcga gcgcatggac cgcgaccgcg ccggcctcgc
     3121 ggtctgatga gcggtcgcgc cccggtctgg gatcccgcgc tcgacgagct cgcgacgacc
     3181 atgcgtgcgg cgatcttcac cggtccgaac gcgccgctgc agctcgggac cgtcgcgcgt
     3241 ccggagcctg ccgccggcga cgtgctggtg aaggtgaccg cctgcggcct gtgccacagc
     3301 gatctccact acatggacca cggcgtgccg acgttcaaga cgccgccgct cgtgctcg
//
LOCUS       DASTZR010000316         4661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_279604, whole genome shotgun sequence.
ACCESSION   DASTZR010000316 DASTZR010000000
VERSION     DASTZR010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279604"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..1363
                     /locus_tag="VFV20_07045"
     CDS             68..1363
                     /locus_tag="VFV20_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288140.1"
                     /translation="MRTLSVTVAALLLAGCVARGEGPLTSMSPSPATVQATASLPPAA
                     ATATAAPSAATAVPLVTPTPRPTPAPVPSCPVAPRPVGDIALVALCPEGAELRIAASL
                     SADDRAAIAAQVTADLVAVQSEFAWRLRTPAVVYVLADRDGYVAALQRVFGYGAATAE
                     FLADNSVAFFEPSLRIIAVDWEEVRDRRPIAAIRHELTHVVTLEACAPRCDLVPAWLN
                     EGQARLAEALMPGSEWRLMRVRYEAASMRATGTLLPLTALWTQAQWNALADWIGYYKY
                     QEAARATELLREDIGDGAIARLYARIRAGQDVPTAYAALAGQSFASFTATLDERIGRG
                     ADAERGIVAITPGAEGGGASFLVYGFPAEATITVHVRAHGIDERSEVKVSPQGAWFGG
                     IDATRPRGAYTITAASGDAVAAVTISKSDGRPLREAPFD"
     gene            complement(1360..2409)
                     /gene="glnII"
                     /locus_tag="VFV20_07050"
     CDS             complement(1360..2409)
                     /gene="glnII"
                     /locus_tag="VFV20_07050"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase GlnII"
                     /protein_id="HEU5288141.1"
                     /translation="MGLDVYRAEYIWIDGTQPTQKLRSKTKIVPKGEKPPIWGFDGSS
                     TQQATGDKSDCVLEPVFVVPDPIRGGDDKLVLCEVMLVSGQPHPTNTRRANVEMAKKY
                     GSFDSWFGIEQEYTFFEGARPLGFPEKGFPAAQGGYYCGVGADEVFGRPVVERHLDMC
                     LKAGLRISGINAEVMPGQWEFQVGPLNAPEIGDQLWIARWLLYRVGEDFGVSATLYPK
                     PVRGDWNGAGAHTNFSTNEMRENYDACIAAADALGERAELHIANYGFGIEERLTGLHE
                     TCSYKEFRYGVSDRGASIRIPWQVAKDRKGYIEDRRPNANADPYVTTRLIIETVCGAA
                     TRKRSAGTAKQAVPA"
     gene            complement(2540..2761)
                     /locus_tag="VFV20_07055"
     CDS             complement(2540..2761)
                     /locus_tag="VFV20_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288142.1"
                     /translation="MRSGGFPCRLAGCDRAFQVLDQNSLDALKTASDARTAHEVTDHG
                     YHHVRLDEERGYAPRIRSSDKAAGKVAGR"
     gene            2883..3071
                     /locus_tag="VFV20_07060"
     CDS             2883..3071
                     /locus_tag="VFV20_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288143.1"
                     /translation="MSRSILGVLSLILFAVGAFYAFNAYIQQRERGFVEVFDVPIAVL
                     GILSGAVLALLASRSRAL"
     gene            complement(3068..3718)
                     /locus_tag="VFV20_07065"
     CDS             complement(3068..3718)
                     /locus_tag="VFV20_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5288144.1"
                     /translation="MRVILADDSVLLRTGLARLLVEAGFEVAGEAGDADGLLALAERE
                     RPDAAIVDIRMPPTHTDEGLRAARELHRRYPAMGVLVLSQHVHPTYAAELLQGGATGM
                     GYLLKDRVSDVRDLTESLQRVASGGCVLDPEVVAQLVGRPRRGDDPLTQLSDREREVL
                     GLMAEGRSNRAIGERLFITEHTVEKHIQAIFAKLALPATADDHRRVLAVLTFLNSG"
     gene            complement(3709..>4661)
                     /locus_tag="VFV20_07070"
     CDS             complement(3709..>4661)
                     /locus_tag="VFV20_07070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU5288145.1"
                     /translation="SAVADLMIELSGTLAPGALQAALSRALHDPSLVIAYWLPEERRF
                     VDATGASAALPDGEDRTVTMVERDGRRIAAFIHDPAVDDEQLMRSACAAAALALENER
                     LQAQLRAQLAEVRSSRARIVEATEEERRRIERDLHDGTQQRLVSIAMTLGLAEARAGN
                     DSAARELIAESRTAMSAALRELRELSQGIHPGILNDRGLAPALDDLVTRLRLPVALEV
                     ALTERLPARVETTAYYVVSEGLTNIAKYAHAASAQVRVRRENGAALIEIADDGAGGAD
                     PSKGSGLRGLRDRVEALGGRFAVTSPPGHGTTIRAELPCV"
BASE COUNT          621 a         1703 c         1635 g          702 t
ORIGIN      
        1 ctcccgctcg cggcgctccg ccgctagacc gtcgcgcggc gggcctcccg gcgttcagca
       61 tgtgcgcatg cgcacgctct cggtgacggt cgccgccctc ctgctcgccg gctgcgtcgc
      121 gcgcggcgag ggaccgctga cctcgatgtc cccgagcccc gccacggtcc aggcgaccgc
      181 gagcctcccg ccggccgcgg ccaccgcgac cgccgcgccg tccgccgcca cggccgtccc
      241 cctggtcacg cccaccccgc ggccgacgcc ggcccccgtg ccgtcgtgcc cggtggcccc
      301 gcgaccggtc ggcgacatcg cgctcgtcgc gctctgtccc gaaggcgccg agcttcgcat
      361 cgccgcatcg ctcagcgcgg acgatcgcgc agccatcgcc gcgcaggtca cggccgacct
      421 cgtcgccgtc cagagcgagt tcgcgtggcg cctgcgcacg ccggccgtcg tgtacgtgct
      481 cgccgatcgt gacggctacg tcgcggcgct ccagcgcgtc ttcggctacg gcgcggccac
      541 cgcggagttc ctcgcggaca actcggtcgc gttcttcgag ccgtcgctgc ggatcatcgc
      601 cgtcgactgg gaggaggtcc gcgaccggcg gccgatcgcc gcgatccgcc acgagctcac
      661 gcacgtcgtg acgctcgagg cgtgcgctcc gcggtgcgac ctcgtccccg cctggctcaa
      721 cgagggccag gcgcggctcg cggaggcgct catgcccggg tccgagtggc gcctcatgcg
      781 cgtgcgctac gaggccgcgt cgatgcgcgc caccggcacg ctgctgccgc tcaccgcgct
      841 gtggacgcag gcgcagtgga acgccctcgc cgactggatc ggctactaca agtaccagga
      901 ggccgcccgc gcgaccgagc tcctgcgcga ggacatcggc gacggcgcga tcgcgcgact
      961 gtacgcgcgg atccgtgccg gacaggacgt ccccaccgcc tacgccgcgc tcgccggcca
     1021 atcgttcgcg agcttcaccg ccacgctcga cgagcgcatc ggccgcggcg cggatgcgga
     1081 gcgcggcatc gtcgcgatca cgccgggcgc cgagggcggc ggcgcgagct tcctcgtgta
     1141 cggcttcccc gcggaggcga cgatcacggt ccacgttcgc gcgcacggta tcgacgagcg
     1201 cagcgaggtg aaggtgtcgc cgcagggcgc gtggttcggc gggatcgacg cgacgcgacc
     1261 gcgaggcgcg tacacgatca cggccgcgag cggcgatgcc gtcgcggccg tgacgatctc
     1321 caagagtgac gggcgccccc tgcgcgaggc gccgttcgac taggccggaa ctgcctgctt
     1381 cgccgtgccg gcggagcgct tgcgcgtcgc cgcgccacac accgtctcga tgatgagacg
     1441 ggtggtgacg tacgggtccg cgttcgcgtt cggccgacga tcctcgatgt agcccttgcg
     1501 gtccttcgcg acctgccacg ggatgcggat cgacgcgcca cggtcggaca cgccgtaccg
     1561 gaactccttg taggagcagg tctcgtgcag gccggtgagg cgctcctcga tgccgaagcc
     1621 atagttggcg atatgcagct ccgcgcgctc cccgagcgcg tcggcggcgg cgatgcaggc
     1681 gtcgtagttc tcgcgcatct cgttcgtcga gaagttcgtg tgggcgccgg cgccgttcca
     1741 gtcgccgcgc acgggcttcg ggtacagcgt cgcgctcacg ccgaagtcct cacccacgcg
     1801 atacaggagc cagcgcgcga tccagagctg gtccccgatc tcgggcgcgt tcagcggccc
     1861 gacctggaac tcccactggc cgggcatgac ctccgcgttg atgccggaga tacggaggcc
     1921 cgccttgagg cacatgtcga gatgccgctc gacgaccggc cggccgaaca cctcgtcggc
     1981 gccgacgccg cagtaatagc ctccctgcgc ggccgggaaa cccttctcgg ggaacccgag
     2041 cgggcgcgcg ccctcgaaga acgtgtactc ctgctcgatg ccgaaccacg agtcgaacga
     2101 gccgtacttc ttcgccatct cgacgttcgc gcgccgagtg ttcgtcgggt gcggctggcc
     2161 gcttacgagc atgacctcgc agagcacgag cttgtcgtcg ccgccgcgga tcgggtccgg
     2221 aacgacgaac accggctcga ggacgcagtc ggacttgtcg cccgtcgcct gctgcgtgct
     2281 cgatccgtcg aagccccaga tcggaggctt ctcgcccttg gggacgatct tcgtcttcga
     2341 ccgcagcttc tgcgtcggct gtgttccgtc gatccagatg tattcggcgc ggtagacgtc
     2401 aagtcccatg cgaggctcct ccaacccaag ttctcagcga acgcgcgaag gcgcggtctc
     2461 gcttcgagtg tcgtgcggga ggcggagagc gtcagtgacg gcggcggtgt ggaagggttg
     2521 cggaagggtg ccgggcggct tagcggccgg ccaccttgcc cgcggccttg tccgacgagc
     2581 ggatgcgggg cgcgtaaccc cgttcctcgt cgaggcggac gtggtggtag ccgtgatcgg
     2641 tcacctcgtg ggccgtgcgg gcatcgctcg cggtcttcag cgcgtcgagc gaattctgat
     2701 cgaggacctg gaacgcacgg tcacagccgg cgagccggca cgggaatcct cctgagcgca
     2761 tgccacatcc ccttccggcg tacagcctac gtgccatagt gccgcatcgc gttgaaacac
     2821 gcgagtggcg gaacggcatc gacgtccggc cgtcgcatac gctgaagcga aggagcgagc
     2881 acatgtcgcg atcgatcctc ggggtgctct cgctgatcct cttcgccgtc ggcgcgttct
     2941 acgcgttcaa cgcctacatc cagcagcggg agcgcggctt cgtcgaagtc ttcgacgttc
     3001 cgatcgcggt cctcgggatc ctctcgggtg ccgtgctggc cctgctggcg tccagatcgc
     3061 gcgcgctcta gccggagttc aggaacgtga gcacggcgag cacgcgacga tggtcgtcgg
     3121 ccgtcgcggg aagcgcgagc ttcgcgaaga tggcctggat gtgcttctcg acggtgtgct
     3181 ccgtgatgaa gagccgctcg ccgatggccc ggttcgagcg gccttcggcc atgagtccga
     3241 gcacctcgcg ttcgcggtcg ctcagctgcg tgagcgggtc gtcgccgcgc cgcggacgcc
     3301 cgacgagctg cgcgaccacc tccgggtcga gtacgcagcc gccgctcgcg acgcgctgca
     3361 ggctctcggt gaggtcccgc acgtccgaga cgcggtcctt cagcaggtag cccatccccg
     3421 tcgcaccccc ctggagcagc tcggccgcgt aggtcggatg gacgtgctgc gagaggacca
     3481 gcacgcccat cgcgggatag cggcggtgca gctcgcgcgc cgcgcgcagt ccctcgtccg
     3541 tgtgcgtcgg cggcatccgg atgtccacga tcgccgcgtc ggggcgctcg cgctcggcga
     3601 gcgccaggag cccgtcggcg tcgccggcct cgcccgcgac ctcgaagcca gcctcgacga
     3661 gcagccgcgc gaggccggta cggaggagca cggaatcgtc ggcgaggatc acacgcatgg
     3721 cagctccgcg cggatggtcg tgccgtggcc cggcggactc gtcaccgcga accgcccgcc
     3781 gagcgcttcg acgcggtcgc gcaggccacg caggccggat cccttcgagg gatccgcgcc
     3841 gcccgcgccg tcgtccgcga tctcgatcag cgcggctccg ttctcacgcc gcacgcggac
     3901 ctgcgcgctg gccgcgtgcg cgtacttcgc gatgttggtg aggccttctg agacgacgta
     3961 gtacgcggtg gtctcgacgc gtgccggcag acgttcggtc agcgcgacct cgagcgcgac
     4021 cggcagacgc aggcgcgtga ccaggtcgtc gagggccggc gcgaggccgc gatcgttgag
     4081 gatcccggga tggatgccct ggctcagctc gcggagctca cgcagggccg cggacatcgc
     4141 ggtgcgcgac tcggcgatca gctcgcgcgc ggcgctgtcg ttccccgctc tggcctcggc
     4201 caggcccagc gtcatcgcga tcgagacgag acgctgctgc gtgccgtcgt gcagatcgcg
     4261 ctcgatccgg cgccgctcct cctccgtcgc ttccacgatg cgcgcgcgcg acgagcgcac
     4321 ctcggcgagc tgcgcgcgca gctgcgcctg caggcgctcg ttctccagcg cgagcgcggc
     4381 cgcggcacac gccgagcgca tgagctgctc gtcatcgacc gcgggatcgt ggatgaacgc
     4441 ggcgatcctg cggccgtccc gctcgaccat cgtcacggtg cggtcctcgc cgtcgggcag
     4501 cgcggcgctc gcgccggtgg cgtccacgaa ccgcctttcc tcgggcagcc agtacgcgat
     4561 cacgagcgat ggatcgtgga gcgcccgcga cagtgccgcc tgcaacgcgc ccggcgcgag
     4621 cgtgccggac agctcgatca tcaggtccgc gaccgcggaa c
//
LOCUS       DASTZR010000317         3748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1271669, whole genome shotgun sequence.
ACCESSION   DASTZR010000317 DASTZR010000000
VERSION     DASTZR010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271669"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VFV20_07075"
     CDS             <1..199
                     /locus_tag="VFV20_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU5288146.1"
                     /translation="GWEETLDGGGITVIEWPDRAGDSLPPDRLDIRLEHIADTKRRVL
                     MEATGPRAEQLLGEFHDSLGA"
     gene            189..917
                     /gene="tsaB"
                     /locus_tag="VFV20_07080"
     CDS             189..917
                     /gene="tsaB"
                     /locus_tag="VFV20_07080"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU5288147.1"
                     /translation="MALDTSTSWASVALYDGRSVLAEETWHAQRRHGDELFPTIERML
                     AHARTTLAEIDRVAVATGPGSFTGLRVAIAAAQGIARGACAALVGVSTLDVLAYPHAP
                     SKQRTCPLLPAGRTEWYAAFYQERNRKWMRRSPYIVATLPDLCRQIGTHTLFVGELDL
                     EAERLLRDLLGPKALFAPPASQMRRAGYLAELGWRALESEPQARLGEIEPVYVKQPAI
                     RVSFDQPFAPAPQDDVALGLPKRT"
     gene            931..1443
                     /gene="rimI"
                     /locus_tag="VFV20_07085"
     CDS             931..1443
                     /gene="rimI"
                     /locus_tag="VFV20_07085"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEU5288148.1"
                     /translation="MVALFPIVIEDMTLDDIDAVQEVERASFPVPWPANAFRHELTQN
                     KNAHYIIAKEGDHVVGYAGLWLSLDEAHITTFAVLPAYRRRRIGERMLLAIFERAGKL
                     GAEWLTLEVRASNLPAQRLYEKYGFRPAGIRRRYYSDNNEDAIIMWTDRLRDRAVRDR
                     IAKLRAALEE"
     gene            1478..2476
                     /gene="tsaD"
                     /locus_tag="VFV20_07090"
     CDS             1478..2476
                     /gene="tsaD"
                     /locus_tag="VFV20_07090"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU5288149.1"
                     /translation="MRILSIETSCDETGVAVVDDGRHIVSNVVASQLAHQETGGIVPE
                     VAAREHLRAVDAITQTALRDAGIELRGVDAIAATVGPGLVGCLLVGANYARGLALASG
                     LPFIGVNHLEGHVYANWLYEGDTAPPEPEIPAIVLVVSGAHSDLVLMEGHRRFRPLGR
                     TIDDAAGEAFDKVARLLGLGFPGGPLIEKAARQGDPNAFPLPRASVADRYDFSFSGLK
                     TAVQRLVRALEAKGEVPVADVAASFQKAITEMLAEKTARAAAEHAVDTVLLGGGVAAN
                     GPLREEIAKRIGRPLVVPPPRLCTDNGAMIGAAAFFVLRHRGTEIPQTVRSDLRLA"
     gene            2473..2904
                     /locus_tag="VFV20_07095"
     CDS             2473..2904
                     /locus_tag="VFV20_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288150.1"
                     /translation="MTRLEEAIEAARVAASSPPNPVSARDAVLAILTAAAEDERVLAN
                     LRDVSAGRALLRFTDTDEAIEFQAGDGKLHAEIADVKGKGPKVDATGATWLGLVSGTL
                     RPWLAFTRGMVVARAGLTELRWLQQVAERLQKAYTEGARRT"
     gene            2916..3692
                     /locus_tag="VFV20_07100"
     CDS             2916..3692
                     /locus_tag="VFV20_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU5288151.1"
                     /translation="MPEAAPAPRRDDTSHPDEQKKVHRAEPKTKTPAEAKRQGPTLDM
                     VKADVRVKTYIRSANAQTGAIGYTEHGERHANTAADGARFILKSLGHEARRCEVAAVA
                     AYVHDIGNVVTREKHGQTGALIAKDILESLNFDVEEIATIMGAIANHEEAEGGLPVSA
                     VSAAVIIADKSDVHRSRVRNPKTTAFDIHDRVNYAATASEIKVSRKEKLITLELTVDT
                     EVAPIMEYFEIFLPRMMLSRRAAEFLHCSFALVINNTRLL"
BASE COUNT          592 a         1316 c         1319 g          521 t
ORIGIN      
        1 cggctgggag gagacgctcg acggcggcgg catcacggtg atcgaatggc ccgatcgcgc
       61 cggcgactcg ctgccacccg atcggctcga catccgcctg gagcacatcg ccgacaccaa
      121 gcgtcgcgtg ctgatggagg ccacggggcc gcgcgcggag cagctgctcg gggagttcca
      181 tgactctctt ggcgcttgac acctcgacgt cgtgggcgtc ggtcgcgctc tacgacggca
      241 ggtccgtgct cgcggaggag acgtggcacg cgcagcgccg gcacggggac gagctcttcc
      301 cgacgatcga gcgcatgctc gcgcacgcac gcacgacgct cgccgagatc gatcgcgtcg
      361 ccgtcgcgac gggacccggc tcgttcacgg gattgcgcgt cgcgatcgcc gcggcgcagg
      421 gcatcgcgcg cggtgcgtgc gcggcgctgg tcggcgtctc gacgctcgac gtgctcgcgt
      481 acccgcacgc gccgagcaag cagcgcacct gcccgctcct cccggcgggc cgcaccgagt
      541 ggtatgccgc gttctatcag gagcgcaatc gcaagtggat gcgccgctcg ccgtacatcg
      601 tcgcgacgct gcccgatctc tgccggcaga tcggcacgca cacgctcttc gtcggcgagc
      661 tcgacctgga ggccgagcgg ctgctgcgcg atctcctcgg accgaaggcg ctcttcgcgc
      721 cgccggcctc gcagatgcgc cgggccggct atctcgcgga gctcggctgg cgtgccctgg
      781 agagcgaacc gcaggcccgc ttgggggaga tcgagccggt ctatgtgaag cagcccgcga
      841 tccgcgtgtc gttcgaccag cccttcgcgc cggcgccgca ggacgacgtg gcactggggc
      901 tgccgaagcg gacatgagaa ggtatgtcgc gtggtagcgc tctttccgat cgtcatcgag
      961 gacatgacgc tcgacgacat cgacgcggtg caggaggtcg aacgcgcctc cttccccgtg
     1021 ccgtggccgg cgaacgcgtt caggcacgag ctcacgcaga acaagaacgc gcactacatc
     1081 atcgccaagg aaggcgacca tgtcgtcggc tacgcgggac tctggctgtc gctggacgag
     1141 gcgcacatca cgaccttcgc ggtcctgccc gcgtatcgcc gccggaggat cggggagcgc
     1201 atgctgctcg cgatcttcga gcgcgcgggc aagctcggcg cggagtggct cacgctcgag
     1261 gtgcgcgcgt ccaacctgcc cgcgcagcgc ctgtacgaga agtacggctt ccggccggcc
     1321 ggcatccgtc gtcgctatta ctcggacaac aacgaggacg cgatcatcat gtggaccgac
     1381 cggctccgcg atcgtgccgt gcgcgaccgg atcgcgaagc tcagggcggc gctcgaggag
     1441 tgaccgagcg cctctaccgc accccgagga gcgggtgatg cggatcctct cgatcgagac
     1501 ctcgtgcgat gagacaggcg tcgccgtcgt cgacgacggc cgccacatcg tgtcgaacgt
     1561 ggtcgcatcg cagctcgcgc atcaggagac cggcgggatc gtgcccgagg tcgcggcgcg
     1621 cgagcatctg cgcgccgtgg acgccatcac gcagaccgcg ctgcgcgacg ctggtatcga
     1681 gctgcgcggc gtcgacgcga tcgcggcgac ggtcgggccc ggccttgtcg ggtgcctcct
     1741 cgtcggcgcg aactacgcgc gcgggctcgc gctcgcgtcg gggctgccgt tcatcggggt
     1801 gaaccacctc gaggggcacg tgtacgcgaa ctggctctac gagggcgaca ccgccccgcc
     1861 cgagcccgag atccccgcga tcgtgctcgt cgtgagcggc gcgcacagcg acctcgtcct
     1921 catggagggc catcgccggt tccgtccgct cggccgcacc atcgacgacg cggccggcga
     1981 ggcgttcgac aaggtcgcgc gtctcctcgg cctcggcttc cccggcggtc cgctcatcga
     2041 gaaggccgcg cgacagggcg acccgaacgc cttcccgctg ccgcgcgcga gcgtcgccga
     2101 tcgctacgac ttctcgttct cgggcctcaa gaccgccgtc cagcggctgg tccgcgcgct
     2161 ggaggcaaaa ggcgaggtgc cggtcgcgga cgtggcggcc tcgttccaga aggcgatcac
     2221 cgagatgctg gcggagaaga ccgcgcgcgc cgcggccgag catgcggtcg acacggtcct
     2281 cctcggcggt ggggtcgccg cgaacggccc gctgcgcgag gagatcgcga agcgcatcgg
     2341 ccggccgctg gtcgtgccgc cgccgcgcct ctgcacggac aacggcgcga tgatcggcgc
     2401 cgcggccttc ttcgtgctgc gtcatcgcgg tacggagatc ccacagaccg tgcgcagcga
     2461 cctgaggctg gcatgacgcg gctcgaggag gccatcgagg ccgcgcgcgt cgccgcgtcc
     2521 tcgccgccga acccggtgag cgcgcgcgat gcggtgctcg cgatcctcac cgcggccgcc
     2581 gaggacgagc gcgtgctggc gaatctgcgc gacgtgagcg ccggccgcgc gctgctgcgc
     2641 ttcaccgaca cggacgaggc catcgagttc caggccgggg acgggaagct gcacgccgag
     2701 atagccgacg tgaagggcaa ggggccgaag gtcgacgcga ccggcgcgac gtggctcggg
     2761 ctcgtctccg ggacgctgcg gccatggctc gcgttcactc gcggcatggt cgtcgcgcgt
     2821 gccgggctca ccgagctccg gtggctgcag caggtcgccg agcgtctgca gaaggcgtac
     2881 acggagggcg cgcgccggac gtagcatcgc gagcgatgcc tgaagccgct cccgcgccac
     2941 gccgcgacga cacgtcgcac ccggacgaac agaagaaggt ccaccgcgcc gagccgaaga
     3001 cgaaaacgcc cgctgaagcg aagcgccagg gtccgacgct cgacatggtg aaggccgacg
     3061 tccgcgtgaa gacgtacatc cgctccgcca acgcgcagac cggcgccatc ggctacaccg
     3121 aacacggcga gcgccatgcg aataccgccg cagacggcgc gcgcttcatc ctgaagtcgc
     3181 tcggtcacga ggcgcgccgc tgcgaggtcg cggcggtcgc cgcgtacgtc cacgacatcg
     3241 ggaacgtggt cacgcgcgag aagcacggcc agaccggagc gctcatcgcg aaggacatcc
     3301 tcgagtcgct gaacttcgac gtcgaggaga tcgcgacgat catgggcgcg atcgcgaatc
     3361 acgaggaggc agagggcgga ttgccggtga gcgcggtgtc ggcggcggtc atcatcgccg
     3421 acaagagcga cgtgcatcgc agccgcgtgc gcaacccgaa gaccacggcg ttcgacatcc
     3481 acgaccgcgt caactacgcc gcgaccgcgt ccgagatcaa ggtgagccgc aaggagaagc
     3541 tgatcaccct cgagctcacg gtcgacaccg aggtcgcgcc gatcatggag tacttcgaga
     3601 tcttcctgcc acgcatgatg ctgtcgcgcc gcgccgccga gttcctgcac tgctcgttcg
     3661 ccctcgtgat caacaacacg cgccttctct aggacgtcgt atggcgcgag ccggtgatcg
     3721 cggcgcatgt ctctcgctcg aagacact
//
LOCUS       DASTZR010000318         2278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1193196, whole genome shotgun sequence.
ACCESSION   DASTZR010000318 DASTZR010000000
VERSION     DASTZR010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193196"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VFV20_07105"
     CDS             complement(<1..606)
                     /locus_tag="VFV20_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEU5288152.1"
                     /translation="MPTYREYFNELKKRVKEATPQQVADLLRRGDIQLADVREKDEWS
                     AGHIPGAVHVPKSFLEQWAEDRIPDKSKTTVLYCAGGVRSAMAAVTLEQLGYTDIISM
                     SGGFNRWKDTGLAWVTPESFTPEQAQRYSRHLLIPEVGESGQHQLLKSKVLLIGAGGL
                     GSPAAYYLAAAGVGTLGIVDSDVVDMTNLQRQILHSSDRVGE"
     gene            704..1186
                     /locus_tag="VFV20_07110"
     CDS             704..1186
                     /locus_tag="VFV20_07110"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEU5288153.1"
                     /translation="MDTLYFDVVRSLYHLALAILVGGGLVLGAAAAPAIFATVRSRGE
                     AGSIFGAVLARWDGLAIFCVILLVITSALMAGIEITGAPEARLIVRWVALGVLGAAVL
                     YASGYASPIARTIRAQTPAWDDMPESAPARREFAKMHRASSSAMRLGIIAGLVAMFLS
                     "
     gene            1189..2010
                     /locus_tag="VFV20_07115"
     CDS             1189..2010
                     /locus_tag="VFV20_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEU5288154.1"
                     /translation="MARPTAIDDALAAHRAKVERAHRVRNATQLVAMVDAVGFCFAFT
                     GEKAYPVPACFDHLSTSDDGKKWEWMWPWKDELAEAKKLYYGKLLVKKPTFVSMKMLP
                     VFYATFGRAGETDDHLDDVRAGRLSDLARRVVEYLAHRGETQKKRMRADLGIESKEGR
                     NDLERAIEDLQRLMYVARVKAVGERSDDYNYTYDLFVRRYPETVKAAERHNSADADAL
                     LLRRLLELAGGVTPKQIGRLFDWDDERVARTTLRLQSSRAVSRADGLLVLADLVR"
     gene            2007..2255
                     /locus_tag="VFV20_07120"
     CDS             2007..2255
                     /locus_tag="VFV20_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288155.1"
                     /translation="MSVTGRAGTPLEWTPLTRADGVVIEQARARNNSWYLVVPAADGS
                     YELRYASQSANTHILSTGQVEEAKRAAETHADRYDTRA"
BASE COUNT          347 a          772 c          798 g          361 t
ORIGIN      
        1 ctcgcccacc cgatcgctgg agtgcaggat ctgccgctgc aggttcgtca tatcgacgac
       61 gtcggagtcg acgatgccga gcgtcccgac acccgccgcc gcgaggtaat aggcggccgg
      121 ggagccgagg ccgccggcgc cgatgagcag caccttgctc ttgaggagct ggtgctggcc
      181 cgactcgccg acctcgggga tgagcaggtg acggctgtac cgctgcgcct gctccggcgt
      241 gaacgactcc ggcgtcaccc acgcgagacc ggtgtccttc cagcggttga acccgcccga
      301 catcgagatg atgtccgtgt agccgagctg ctcgagcgtg acggcggcca tggcggatcg
      361 cacgccgccc gcgcagtaga ggacggtggt cttgctcttg tccgggatgc gatcctccgc
      421 ccactgctcg aggaagctct tgggcacgtg caccgcgccg gggatgtggc cggcgctcca
      481 ctcgtccttc tcacgcacgt cggcgagctg gatgtcgccg cggcgcagca ggtcggcgac
      541 ctgctggggc gtcgcttcct tgacccgctt cttgagttcg ttgaagtact cgcgataggt
      601 gggcatcggc acgctccctt accgcgattc taaaccgacc atgtccgtcg gaaattccga
      661 gcagtgcagc gtgcggtgtc gccgggcgat atggtcgaag cgcgtggaca ccctctattt
      721 cgacgtcgtc cgctccctct atcacctcgc gctcgcgatc ctcgtcggcg gcggtctcgt
      781 cctcggggcc gccgccgccc cggcgatctt cgccacggtg cgctcgcgcg gcgaggcggg
      841 ctccatcttc ggcgcggtgc tcgcccggtg ggacggcctc gccatcttct gcgtgatcct
      901 gctcgtgatc acgagcgcgc tcatggccgg gatcgagatc accggcgcac ccgaggcgcg
      961 tctcatcgtc cggtgggtcg cgctcggcgt gctcggcgcc gcggtgctct acgcgagcgg
     1021 gtacgcgagc cccatcgctc ggacgatccg cgcgcagacg ccggcctggg acgacatgcc
     1081 cgagagcgcg ccggcgcggc gggagttcgc gaagatgcat cgcgcgtcgt cgagcgcgat
     1141 gcggctcggc atcatcgcgg gcctcgtcgc gatgttcctg tcctgacgat ggcgcggccg
     1201 acggccatcg acgacgcgct cgcggcccac cgcgccaagg tcgaacgcgc gcaccgcgtg
     1261 cggaacgcca cgcagctcgt cgccatggtc gacgccgtgg gattctgctt cgccttcacc
     1321 ggcgagaagg cgtatccggt gcccgcgtgc ttcgaccacc tctccacgag cgacgacggc
     1381 aagaagtggg aatggatgtg gccgtggaag gacgagctcg cggaagcgaa gaagctctac
     1441 tacggcaagc tgctggtgaa gaagccgacg ttcgtctcga tgaagatgct gccggtgttc
     1501 tacgcgacct tcggccgggc aggcgagacc gacgaccatc tcgacgacgt gcgcgcgggc
     1561 cggctgtcgg atctcgcgcg ccgcgtcgtg gagtacctcg cgcatcgcgg cgagacgcag
     1621 aagaagcgaa tgcgtgcgga tctgggcatc gaatcgaagg aagggcgcaa cgatctcgag
     1681 cgcgcgatcg aggacctgca gcgcctcatg tacgtcgcgc gcgtgaaggc ggtcggcgag
     1741 cgcagcgacg actacaacta cacgtacgac ctcttcgtgc gacgctatcc cgagacggtg
     1801 aaggcggcgg aacggcacaa ctcggcggac gcggacgcgc tgctcctgcg gcggctcctc
     1861 gagctcgccg gcggcgtgac gccgaagcag atcggccggc tcttcgattg ggacgacgag
     1921 cgcgtcgcgc gcaccactct gcggttgcag agctcgcgcg ccgtgtcgcg cgccgacgga
     1981 ctgctggtgc tcgcggatct ggtccgatga gcgtgacggg gcgcgcgggc acgccgctcg
     2041 agtggacgcc gctcacgcgt gccgacggcg tcgtcatcga gcaggcgcga gcacggaaca
     2101 actcctggta tctcgtcgtg ccggccgccg atggctcata cgagctgcgc tacgcgagcc
     2161 aatcggccaa cacgcacatc ctctcgaccg gtcaggtcga ggaggccaag cgcgcggcgg
     2221 agacccacgc cgaccggtac gatacccgcg cgtgatgggt atatcggggc gagcgata
//
LOCUS       DASTZR010000319         2489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_196992, whole genome shotgun sequence.
ACCESSION   DASTZR010000319 DASTZR010000000
VERSION     DASTZR010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196992"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..>2489)
                     /locus_tag="VFV20_07125"
     CDS             complement(177..>2489)
                     /locus_tag="VFV20_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEU5288156.1"
                     /translation="QDFLDKARAIQHYGAAVVVMAFDETGQADTVERKVAICQRAYKL
                     LIERAGFEPSDIIFDPNILAIATGLEEHNDYAVNYIEATRIIKATCPGVKVSGGVSNL
                     SFSFRGNDAVREAMHSAFLYHAIAAGMDMGIVNAGQLVVYEDIPKDLLEHVEDVIFNR
                     RPDATERLVQFADSVKGAGKKREVDVAWRGGTVQARLSFALVHGVVDFIEADVEEARQ
                     QYARPLEVIEGPLMDGMKIVGDLFGAGKMFLPQVVKSARAMKKAVAVLLPYMEEEKLR
                     TGASQAQGKILMATVKGDVHDIGKNIVGVVLGCNNYEVIDLGVMVPAAKILDTAIEQK
                     VDIIGLSGLITPSLDEMVFVAREMERRGFTIPLLIGGATTSPQHTAVKIAHEYSGPVV
                     HVLDASRAVDVVSSLIGEKREEFDKANRVAQENTRQKYAGRKEKPLLSIADARANRLK
                     TNWDEHVIATPWFLGRRFLPDVSLEEIARYIDWTFFFTAWELKGRFPAILDHPQYGKA
                     ARDLYENAQALLKRIMSEKLLHASGVYGFWPANALGDDIVVFTNDDRRDELVRLPMLR
                     QQEVIADGRPNRSLADFIAPLESGVPDYIGLFAVTAGLGAEALVKQFERDNDDYNAIM
                     VKALADRLAEAFAEYLHAQARKDWGYGADERLTNEDLVAEKYRGIRPAYGYPACPDHS
                     EKFKLFDVLEARKAGLELTESAAMTPAASVSGLYFSHPEAKYFIVGRIGRDQLEDYAR
                     RKQAPLEEVERWLAPNLAYDPSATPVGQPG"
BASE COUNT          386 a          877 c          773 g          453 t
ORIGIN      
        1 gctccggtta tcagacggcc tgctcctgcg attggttggc ggaatccgcg cgatttcgcc
       61 tgaaaattac aggcttagac accgcatcgt cgcattcggc gctccggacc cggcgctgac
      121 acgaatacgc ccattggaga cgtctcgtta ccggcggcga cgggagcaga aggacgtcag
      181 ccgggctggc cgaccggcgt cgccgacgga tcgtacgcga ggttcggcgc gagccatcgc
      241 tcgacctcct cgagcggcgc ctgcttgcgg cgcgcgtaat cctcgagctg atcccgcccg
      301 atgcgtccca cgatgaagta cttcgcctcc gggtgcgaga agtagaggcc gctgaccgac
      361 gcggcgggcg tcatcgccgc gctctcggtc agctccaggc ccgccttgcg cgcctcgagg
      421 acgtcgaaca gcttgaactt ctcgctgtgg tccggacacg cgggataccc gtacgccgga
      481 cggatgccgc ggtacttctc cgcgacgaga tcctcgttcg tcagccgctc gtccgcaccg
      541 tagccccagt ccttccgcgc ctgcgcgtgg aggtattccg cgaacgcctc ggcgaggcgg
      601 tcggccagcg ccttcaccat gatcgcgttg tagtcgtcgt tgtcccgctc gaactgcttc
      661 acgagcgcct ctgcgcccag gccggccgtg acggcgaaca ggccgatgta gtcggggacg
      721 ccggattcca gcggcgcgat gaagtcggca agcgagcggt tcggacggcc gtccgcgatg
      781 acctcctgct gccgcagcat cggcaggcgc acgagctcgt cgcgccgatc gtcgttcgtg
      841 aagaccacga tgtcgtcgcc gagcgcattc gccggccaga agccgtagac gccgctcgcg
      901 tgcagcagct tctcgctcat gatgcgcttc aggagcgcct gcgcattctc ataaaggtcg
      961 cgcgccgcct tcccgtactg cgggtggtcg aggatcgcgg ggaaacgccc tttgagctcc
     1021 cacgccgtga agaagaacgt ccagtcgatg tagcgcgcga tctcctcgag ggagacgtcc
     1081 gggaggaacc ggcggccgag gaaccagggc gtcgcgataa cgtgttcgtc ccagttcgtc
     1141 ttcagccggt tcgcacgcgc gtccgcgatc gacagcagcg gcttctcctt ccgcccggcg
     1201 tacttctgac gcgtgttctc ctgcgcgaca cggttcgcct tgtcgaactc ctcgcgcttc
     1261 tcgccgatca ggctggacac cacgtccacc gcccgcgacg cgtcgagcac gtgcacgacc
     1321 ggcccgctgt actcgtgcgc gatcttcacc gccgtatgct gcgggctcgt cgtcgcgccg
     1381 ccgatgagga gcgggatcgt gaagccgcgc cgctccatct cgcgcgcgac gaacaccatc
     1441 tcgtcgagcg acggcgtgat cagccccgac agcccgatga tgtccacctt ctgctcgatg
     1501 gcggtatcga ggatcttcgc cgccggcacc atgacaccga gatcgatgac ttcatagttg
     1561 ttgcagccga ggacgacccc gacgatgttc ttcccgatat cgtggacgtc gcccttcacc
     1621 gtggccatga ggatcttccc ctgcgcctgc gaggcgccgg tccgaagctt ctcctcctcc
     1681 atgtacggca gcagcaccgc gaccgccttc ttcatcgcgc gggcgctctt gaccacctgc
     1741 ggcaggaaca tcttcccggc gccgaacagg tcgccgacga tcttcatgcc gtccatcaac
     1801 ggcccctcga tcacctccag cggacgcgcg tactgctgac gcgcctcctc cacgtccgcc
     1861 tcgatgaagt ccacgacgcc gtggaccagc gcgaacgaca gccgcgcttg aacggtgccg
     1921 ccccgccacg cgacgtccac ttcgcgcttc ttccccgccc ccttcacgct gtcggcgaat
     1981 tgcaccagcc gctcggtcgc atcgggccgg cgattgaaga tgacgtcctc cacgtgctcg
     2041 agcaggtcct tcgggatgtc ctcgtagacg accagctgcc cggcattgac gatgcccatg
     2101 tccatgccgg cggcaatcgc gtggtagaga aacgccgagt gcatcgcttc gcgtaccgcg
     2161 tcgtttccgc ggaacgagaa cgacaggttg ctcacgccgc cgctcacctt cacgcccggg
     2221 caggtcgcct tgatgatccg cgtggcctcg atgtaattga ccgcgtagtc gttgtgctcc
     2281 tccagcccgg tcgcgatcgc gaggatgttc gggtcgaaga tgatgtcgga cggctcgaac
     2341 ccggcgcgct cgatgagcag cttgtaggcg cgctggcaga tcgccacctt tcgttcgacg
     2401 gtgtcggcct gaccggtttc gtcgaacgcc atgacgacga ccgcggcgcc gtagtgttgg
     2461 atggcccgcg ccttgtcgag gaagtcctg
//
LOCUS       DASTZR010000320         5512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1062932, whole genome shotgun sequence.
ACCESSION   DASTZR010000320 DASTZR010000000
VERSION     DASTZR010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5512
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062932"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VFV20_07130"
     CDS             complement(<1..579)
                     /locus_tag="VFV20_07130"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HEU5288157.1"
                     /translation="MSPLRGEEFTGRTVEEAIERGLRALGRKRTEVDIEVLEKGKPAN
                     VLGMGGEDARVLLSYQEEERAEEDEPAIDEAEVPRRPDVVREQRETHDEEEASPAHAE
                     ELALGAEVLRGILTEMGIEAEVTHGEAAQGDGVEVVGSDLGALIGRGGENLVALQQLV
                     SAITSKRVGRSVHVGIDIEGYRRRREEQLKEMT"
     gene            complement(576..1550)
                     /locus_tag="VFV20_07135"
     CDS             complement(576..1550)
                     /locus_tag="VFV20_07135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HEU5288158.1"
                     /translation="MNFLAGPWDTFLVHPLMALAILAYSIAHDFGLAIIIVTVVIRLL
                     VYPLYVTQIRSQRAMQELGPAMNELKKKYGKDRQRFAEEQMKLYKERGVNPAAGCLPL
                     LLQMPLLFALYSALAQLGCGLGNSIGAACASTLTHDQAQQVMLSFFPNPIPEGGTLDT
                     TANWLPWISNGLHERDPLYILPVLAGVAQLVASVMAMPAKRVQTDDPNQKMFQSMAYT
                     FPIMTVVLGASFPAGLTLYWIATTIFQIVQQYFVSGWGQLPKYLPFLRNIPSPADRAM
                     RRETQEIIEEGQHDLEGAPALAPSGGAADDANAARGQGRRRGRRRGKR"
     gene            complement(1547..1768)
                     /gene="yidD"
                     /locus_tag="VFV20_07140"
     CDS             complement(1547..1768)
                     /gene="yidD"
                     /locus_tag="VFV20_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007086500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEU5288159.1"
                     /translation="MKGLVLGLLRFYKARISPQLPPACRYTPTCSEYAMEAVERHGVV
                     VGGFLAMKRVLSCNPFSRGGYDPVPERAR"
     gene            complement(1765..2100)
                     /gene="rnpA"
                     /locus_tag="VFV20_07145"
     CDS             complement(1765..2100)
                     /gene="rnpA"
                     /locus_tag="VFV20_07145"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEU5288160.1"
                     /translation="MNRAARLRRSSDIALVRAEGRAVRRAGFVARVRRHEGPELRLAV
                     VAPGSVGGSVTRNRVRRRVREAFRRAIREGTRTSGADLLVTARREAAAADFKALEADA
                     ASLLREAIR"
     gene            complement(2111..2251)
                     /gene="rpmH"
                     /locus_tag="VFV20_07150"
     CDS             complement(2111..2251)
                     /gene="rpmH"
                     /locus_tag="VFV20_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006886182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU5288161.1"
                     /translation="MVKRTYQPKTRRRARVHGFRARMKTTGGRKVLAARRARGRKRIT
                     VV"
     gene            2506..3876
                     /gene="dnaA"
                     /locus_tag="VFV20_07155"
     CDS             2506..3876
                     /gene="dnaA"
                     /locus_tag="VFV20_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU5288162.1"
                     /translation="MNLKQLWSAALGEIQLSLSKANYETWFKDTAIVSEEDDVFMIGV
                     PNAFAREWLENKYRTQVRETLQRLLSRTVDVRFVNMTASSARSDRPVASVPAAATAGS
                     GQAAPERPRDNPAGALLNPRYTFSTFVVGSNNRLAHAAALSVAERPGHSYNPLFIYGG
                     SGLGKTHLMHAIGHAVTGRHPKKRVAYATSEKFTNEFINSIRAQKGEEFRERYRRIDV
                     LLIDDIQFLTGKEGTQEEFFHTFNAIHEEGKQIVLSSDRPPKAIERLEDRLRSRFEWG
                     LIADISSPDLETRIAILRAKAEAQSVPVPPPVIDFLAQRIVSNIRELEGALTRIVAYA
                     TLNAVPVTTQLAQEILQNILYNPRQKTLSPEKIVETVARYYGVPSEQLRGKARDKQIV
                     LPRQIAMYLMREETEAPLLRIGEALGGRDHSTVLHGCEKIEREMAENDDFRRDVGALR
                     EMLYSD"
     gene            4256..5383
                     /gene="dnaN"
                     /locus_tag="VFV20_07160"
     CDS             4256..5383
                     /gene="dnaN"
                     /locus_tag="VFV20_07160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU5288163.1"
                     /translation="MKVTCLQENLARGLQIAGRAVSTRGSLPILGNVLLRTENGRMKL
                     TATNLEVGINVWVPAKVDDEGAITVPAKLFTDFVNSLPPGPAELSLNVRTKTVHLRSG
                     PYEANFKGMDAEEFPIIPVAPDKPTTRLAKSTLRRMIGEVAFVATTDDSRPVLTGVLT
                     TFDADRITMAAADPYRLSVRTGQLVSKVESKLEVIIPARSLQEVQRIIDDTDDPVDIF
                     VTPNGSQVIFHTEEADLVSRVIEGQFPNFRQVIPTGKAATRIIVQREELLQATRLASL
                     FARDSANMLRFQVDPSAQPPLIISANAAEVGDNTAKVDATVEGQNTTIAFNSRFIADA
                     LGSLSAPEVVLELGGPLAPGVVKIVGDDSYLHVVMPLRIPA"
     gene            5385..>5512
                     /locus_tag="VFV20_07165"
     CDS             5385..>5512
                     /locus_tag="VFV20_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003967888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5288164.1"
                     /translation="MRVTDLALEDFRSYAEVQLAFGRGVTAFLGPNGAGKTNLLEA"
BASE COUNT          933 a         1891 c         1798 g          890 t
ORIGIN      
        1 ggtcatctcc ttgagctgct cctcgcggcg acgacggtac ccctcgatgt cgatgccgac
       61 gtgcacggag cgccccaccc gcttcgacgt gatcgcgctc acgagctgct gcagcgcgac
      121 gaggttctct ccaccgcgtc cgatgagcgc gccgaggtcg cttcccacga cctccacgcc
      181 gtcgccctgc gcggcctccc cgtgtgtcac ctccgcctcg atgcccatct ccgtcaggat
      241 cccgcggagc acctcggcgc cgagcgcgag ttcctccgcg tgggccggcg acgcctcttc
      301 ctcgtcgtgg gtctcgcgct gctcacgcac cacgtcggga cggcgcggga cctcggcctc
      361 gtcgatcgcg ggctcgtctt cttccgcgcg ctcctcttcc tggtaggaga gcagcacgcg
      421 agcatcctcg ccgcccatgc ccagcacgtt cgcgggcttg cccttctcga gcacctcgat
      481 gtccacctcg gtccgtttgc gccccagcgc ccgcaggccg cgttcgatcg cctcctcgac
      541 ggtccgcccg gtgaactcct cgccgcgtag tggactcatc gcttccccct ccgccgtcca
      601 cggcgtcgcc cctgtcctcg tgctgcgttc gcgtcatcgg cggccccgcc gctcggggcg
      661 agcgcgggcg cgccctcgag gtcgtgctgc ccttcctcga tgatctcctg cgtctcgcgg
      721 cgcatggcgc ggtccgcggg cgacgggatg ttacgcagga atggcaggta cttgggcagc
      781 tgcccccagc cgctcacgaa gtactgctgc acgatctgga agatcgtcgt cgcgatccag
      841 tacagcgtga ggccggcggg gaacgacgcg ccgagcacca cggtcatgat cgggaacgtg
      901 tacgccatcg actggaacat cttctggttc gggtcgtcgg tctggacgcg cttggccggc
      961 atcgccatca ccgacgcgac gagctgcgcg acgcccgcga ggaccggaag gatgtagagc
     1021 gggtcgcgct cgtgcagtcc gttggagatc cacggcagcc agttggcggt cgtgtcgagg
     1081 gtgccgccct ccgggatcgg attggggaag aacgacagca tgacctgctg cgcctggtcg
     1141 tgcgtcagtg tcgacgcgca cgcggcgccg atcgaattcc cgagcccgca gccgagctgc
     1201 gcgagcgcgg agtacagcgc gaagaggagc ggcatctgca gcaggagcgg caggcagccg
     1261 gcagcggggt tcacgccgcg ctccttgtag agcttcatct gctcctcggc gaagcgctgg
     1321 cggtccttgc cgtacttctt cttgagctcg ttcatcgccg gtccgagctc ctgcatcgcg
     1381 cgctgcgagc ggatctgtgt cacatacagc ggatacacga ggagccggat gacgacggtc
     1441 acgatgatga tcgcgagccc gaagtcgtgg gcgatgctgt acgcgaggat cgcgagcgcc
     1501 atgagcggat ggacgaggaa cgtgtcccag gggcccgcga ggaagttcat cgggcgcgct
     1561 ccggcaccgg gtcatacccc ccgcggctga aggggttgca cgagagcaca cgcttcatcg
     1621 cgaggaatcc gccgacgacc acgccgtgcc gctcgaccgc ctccatcgcg tactccgagc
     1681 atgtcggggt gtagcggcag gcgggcggca gctgggggct gatacgggcc ttgtagaagc
     1741 gcagaagccc cagcaccagg cccttcaacg gatggcctca cggagcaggc tcgccgcgtc
     1801 ggcctcaagc gccttgaagt ccgcggccgc ggcctcgcgc cgggccgtca cgagaaggtc
     1861 cgcacccgac gttcgcgtcc cctcgcgtat ggcgcgccgg aacgcctcac gtacgcgacg
     1921 gcggacacga ttgcgcgtga ccgaaccgcc caccgagccg ggcgcgacga cggcgagcct
     1981 gagctcaggc ccctcatgac gacggacgcg tgcgacgaag ccggctcgcc gcacggcgcg
     2041 accttccgct cgcaccaggg cgatgtcgct ggaacgccgc aatcgcgcgg cgcggttcat
     2101 tcgactgtga tcagacgacg gtgatgcgct tgcggccacg cgcgcgacga gcggcgagga
     2161 ccttgcggcc accggtcgtc ttcattcgcg cgcggaatcc atgcactcgc gcacgacgac
     2221 gcgtcttcgg ctgatatgtg cgcttcacga tcgagtccct tctccccgga ggaacagcgg
     2281 atcaccgctg aaacgaaata gaacagggtg tgtgtgtccc agtcaggata catggcgcga
     2341 cgtgtgctcg ctcgtcgcac ggttgcccgc caattcgacg cggtgctact gttcggcggc
     2401 ttccagcggg cggatctggg tggatctctc ttcggtgtgg caactcgcga gcgcaacgtg
     2461 tgtggggagc atcgtctttt gttcgagcgg agagaagggg aacctctgaa cctcaaacag
     2521 ctgtggtccg ccgccctcgg tgagatccag ttgtcgctct caaaagcgaa ctacgagacc
     2581 tggttcaagg acaccgcgat cgtctccgaa gaggacgatg tcttcatgat cggcgtccca
     2641 aacgccttcg cccgcgaatg gctcgagaac aagtaccgca cccaggtgcg cgaaacgctc
     2701 cagcggctcc tgtcgcggac cgtcgacgtt cgtttcgtga acatgaccgc gtcgtcggcg
     2761 cgctccgacc gtccggtcgc gtccgtgccg gccgccgcga cggccggatc cggccaggcc
     2821 gctccggagc ggccccgtga caacccggcg ggggccttgc tgaatcctcg atacacgttc
     2881 tccacgttcg tcgtcggctc gaacaacagg ctggcgcacg ccgcggcgct ttccgtggcc
     2941 gagcgtcccg gccattccta caaccccctc ttcatctacg ggggttctgg cctcggaaag
     3001 acccatctga tgcacgcgat cggtcacgcc gtcaccggcc gccatccgaa gaagcgggtc
     3061 gcgtacgcga cgagcgagaa gttcaccaac gagttcatca actcgatccg cgcgcagaag
     3121 ggcgaggagt tccgggagcg ctaccggcgg atcgacgtgc tcctcatcga cgacatccag
     3181 ttcctgaccg gcaaggaggg cacgcaggag gagttcttcc acaccttcaa tgccattcac
     3241 gaggagggca agcagatcgt gctctcgagc gatcggccgc ccaaggcgat cgagcgcctc
     3301 gaggaccggc tgcgctcgcg gttcgagtgg gggctcatcg cggacatcag ctcgccggac
     3361 ctggagacgc gcatcgcgat cctccgcgcg aaggccgagg cgcagagcgt cccggtaccg
     3421 ccgccggtca tcgacttcct cgcgcagcgc atcgtctcga acatccgcga gctcgagggt
     3481 gcgctcacgc gcatcgtcgc ctacgccacg ctcaacgccg tgcccgtcac gacgcagctc
     3541 gctcaggaga tcctccagaa catcctctac aacccgcgcc agaagacgct gtctcccgag
     3601 aagatcgtcg agaccgttgc gcgctactac ggcgtgccgt ccgagcagct gcgcggcaag
     3661 gcgcgggaca agcagatcgt cctgccgcgg cagatcgcga tgtatctcat gcgcgaggag
     3721 acggaggcgc cgctgctgcg catcggtgaa gcgctcggcg gccgcgacca ctccacggtg
     3781 ctgcacggct gcgagaagat cgagcgtgag atggccgaga acgacgactt ccggcgtgac
     3841 gtcggagcgc tgcgggagat gttgtactcg gactagccgc tggcgagcga ggcgaggatg
     3901 ccggcgaccg tcgaaaaacc cgtgaaaatc gcgccgattc tcgtgctccg gcacgtgtgg
     3961 ataaggccac cgaatgtgtg gataagcgca cccatttgtg gacaggtacc cctcaagtgg
     4021 gtgccgagga ccgtcatgtg tcgcgagttg ggacatcagc cttacagtgt ccacacccgt
     4081 cccccgcgca tccccgcgac gtccaccgcc cgcgaggagc aggcagcgag gcgtcagggg
     4141 ccatgccggg cagggcggga cgcgacatca gcatctcgga agtcgcatag tccacaccac
     4201 tactgctagt gactactgcg tatatctaag acgaacggaa ggacggggag ggcgcgtgaa
     4261 ggtcacttgt ctacaggaga acctcgcccg aggactccag atcgccgggc gcgcggtatc
     4321 gacgcgtgga agcctgccca tcctcggcaa cgtgcttctc cggaccgaga acggacggat
     4381 gaagctgacc gcgacgaacc tcgaggtcgg gatcaacgtc tgggtcccgg ccaaggtcga
     4441 cgacgagggc gcgatcaccg tccccgccaa gctgttcacc gacttcgtga actcattgcc
     4501 gcccggaccg gccgagctgt ccctgaacgt ccgcaccaag accgtgcatc tgcgctcagg
     4561 accgtatgag gcgaacttca agggtatgga cgccgaggag ttcccgatca tcccggtcgc
     4621 acccgacaag ccgacgaccc ggctcgcgaa gtcgacgctg cgccggatga tcggcgaggt
     4681 cgcgttcgtc gccaccacgg acgacagccg gccggtgctc acgggcgtgc tcacgacgtt
     4741 cgatgccgat cgcatcacga tggcggccgc ggatccgtat cgactctccg tgcgcacagg
     4801 tcaactcgtg tcgaaggtcg agagcaagct cgaggtcatc atccccgcgc gcagcctgca
     4861 ggaggtccag cggatcatcg atgataccga tgatccagtg gatatcttcg tgacgccgaa
     4921 tggcagccag gtcatcttcc acaccgagga ggccgacctc gtctcccgtg tcatcgaggg
     4981 gcagttcccc aacttccggc aggtgatccc gaccggcaag gccgccaccc ggatcatcgt
     5041 gcagcgtgaa gagctgctcc aggcgacacg gctcgcgtcg ctgttcgcac gcgactcggc
     5101 caacatgctg cggttccagg tcgatccgag cgcgcagccg ccgctgatca tcagtgcgaa
     5161 cgccgctgag gtcggggaca acacggcgaa ggtcgacgcg acggtggaag gccagaacac
     5221 gacgatcgcc ttcaactcgc ggttcatcgc cgatgcgctc gggagcctct ccgctccgga
     5281 ggtcgtgctc gagctcggcg gaccgctcgc accgggtgtc gtgaagatcg tcggcgacga
     5341 cagctacctc cacgtggtga tgccgctccg catccccgcg taaggtgcgc gtcaccgacc
     5401 tcgcgctcga ggacttccgc tcgtacgcgg aggtacagct cgccttcggc cgcggcgtca
     5461 ccgcgttcct cggtccgaac ggggcgggga agacgaacct cctcgaggcg at
//
LOCUS       DASTZR010000321         5414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_123060, whole genome shotgun sequence.
ACCESSION   DASTZR010000321 DASTZR010000000
VERSION     DASTZR010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123060"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2211)
                     /locus_tag="VFV20_07170"
     CDS             complement(<1..2211)
                     /locus_tag="VFV20_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEU5288165.1"
                     /translation="MTEALGLTAVEARRRLDVFGPNLLVPEHEATGLWATLRHIVTDP
                     MAILLVVAAVTYFALGETRDGVIALVALAPIVGVGLVLGLRAEHALESLRRLTAPLAR
                     VIRDGREELVPALDLVPGDAIVLREGDVIPADARLVSGAQLVVDESALTGESQALTKT
                     VLNEEEAALWAGTTVLAGRATAVIAVTGAKTRYGTIGELVARIAPSPTPLQQVIRKLF
                     VQLSVVAAAFCVGVIVIELARGHSAAEALIAGVSLAMAAIPEEFPMVFTLYLGLGAWR
                     LARDHALVRRLVGVETLGATSVICADKTGTLTLGTIEVADQWAVAGVTERDLLTLALL
                     ASEPEPFDPLEQAIVRAAATHGIDVSRVHAAELVRDHEFDPVTRTLTHVWRRDGVLGA
                     YAKGALEGILSHGVTAADVRDGATKANDDLAARGMRVIAVATSDQAVENGDRSSDEAR
                     LRLAGLLAFVDPLRPGVDASIAECREAGVRVVMITGDHPITARAVAEGLGMPQARVAT
                     GTDIDAASDEELRSLVASTDVFARARPEQKYRLVRAFRDGGEVVAMTGDGTNDAPALR
                     EADIGIAMGRRGTEVARSAATLVLLDDDFSTIVAAVRDGRRIFDNLRRAFSYLVSFHA
                     PLLLSAIVVPLTGAPLLLLPVHLVFLELVLHPTVALVFEGDAADPDLMRRPPRGRATG
                     LLHGSQVAGVVARGVALTVGVLAVYLIQLPVLGEESARGIAFATMVIGQLLLVIV"
     gene            complement(2214..3656)
                     /locus_tag="VFV20_07175"
     CDS             complement(2214..3656)
                     /locus_tag="VFV20_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEU5288166.1"
                     /translation="MAILTEVQPHAVLTPAYRLVFRLEIANRPGMFAHVALTIGARGC
                     SLGAIDLVEATSAVHVRDVTVDCPDEQTGQKLLHDLQALDGVRVRSVSDRAFLLHLGG
                     KIEIKGRVQVRTRDDLSLVYTPGVARICRAIAEDQQSVWKLTIRQNTVAVVTDGTAVL
                     GLGDIGPEAAMPVMEGKALIFSEFAGITAFPLCLATKDPDEIVRTVQHVAPVFGGINL
                     EDIAAPSCFIVERKLRETLDIPVFHDDQHGTAVVVLAALRNAAAVVQREIRDLRVVIA
                     GAGAAGIATARLLHLAGVREIVLCDRHGIVNAARSDQNAEKVAIARELGTQRAGTLED
                     AVAGANVFIGLSGPRVLTIDALRTMARPRIVFALANPDPEIDPLVAASEVDVLATGRS
                     DYPNQVNNSLVFPGVFRGLLDAHARGVSPDIELAAAAALAATVPADLRSAEYILPSMF
                     DREVVPQVAHAVAAATGASGLSRRTPALEP"
     gene            3717..3959
                     /locus_tag="VFV20_07180"
     CDS             3717..3959
                     /locus_tag="VFV20_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288167.1"
                     /translation="MPFGTWTPVRRAQITGMVSMDRQPVSPKTRLVQCPLRDRQVAIE
                     KCLQCGRLIDKDSEVPPRYVVCDARVVTGWLGLDDL"
     gene            4030..5334
                     /locus_tag="VFV20_07185"
     CDS             4030..5334
                     /locus_tag="VFV20_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288168.1"
                     /translation="MRSALILILSLIGIAPAAPGTAAPATATATCTSSVGPGIPPPAR
                     VPGGIPGFHAAWYGQSGYMRLCPGERSTATVAYYNSGSRGWVSGRMGEVAYLGSWDSE
                     PGQDQPSVLGGDGQLGSPNTAWPRFNRAALQPAPYVGPGQVAWFQFSVQAPARPGTYR
                     LGIRPLVEGAQWMEDYGVYWEITVLNPDGTTPLATPPSPAGFYYATGAGVGAMDIADV
                     HEGVEWISSYLAANAGGDRRRIATARIATGDGTEQYCCITQGPTFDIVTSNRAWSSPP
                     AAAADTWTPDTERIELAAHEYVHVWQYDTGGRACMVGGPRWISEGMAESLAYRSLVAA
                     GRIPAANMDTFTKRQLRSAQYVTLRSLESTWPSAANPFAVAYLAVDRLLAQPSPLALR
                     TYCARVGAGELWQTAFAAAFGTDADTFYTRFEAYRTQYLATP"
BASE COUNT          806 a         1927 c         1954 g          727 t
ORIGIN      
        1 cacgatgacg agcaggagct gtccgatgac catggtcgcg aaggcaatgc cgcgcgcgct
       61 ctcctcgccc agcacgggga gctggatgag gtagaccgcc agcacgccga cggtgagcgc
      121 gaccccacgc gcgacgacac ccgccacctg ggacccgtgg agcaagcccg tcgcgcgccc
      181 gcgcggcggc cggcgcatga gatcgggatc cgcggcgtcg ccctcgaaca cgagcgcgac
      241 cgtcggatgc agcacgagct cgaggaacac gagatggacc gggagcagga ggagcggggc
      301 gccggtcagc ggcacgacga tcgccgacag cagcaggggc gcgtggaacg acaccaggta
      361 gctgaacgcc cggcgcaggt tgtcgaagat gcgccgcccg tcgcgcaccg cggcgacgat
      421 cgtggagaag tcgtcgtcga ggaggacgag cgtggcggca ctccgggcca cctcggtccc
      481 acggcggccc atggcgatcc cgatgtccgc ctcacgcagc gcgggcgcgt cattcgtgcc
      541 gtccccggtc atcgcgacga cctcgccgcc gtcgcggaac gcgcgcacca ggcggtactt
      601 ctgctccggc cgggcacggg cgaacacgtc ggtggacgcg acgagcgaac gcagctcctc
      661 gtcggaggcc gcgtcgatgt cggtgcccgt cgcgacgcgt gcctgcggca tgccgaggcc
      721 ctcggcgacc gcgcgtgcgg tgatcgggtg gtcgccggtg atcatgacca cacgcacgcc
      781 cgcttcgcgg cactcggcga tcgacgcgtc gacaccggga cgcagcggat cgacgaacgc
      841 gagcaggcct gccagacgca gccgggcttc gtcgctcgag cgatcgccgt tctcgacggc
      901 ctggtcactc gtcgccaccg cgatcacgcg catgccgcga gccgcgagat cgtcgttggc
      961 cttcgtcgcg ccgtcgcgga cgtcggccgc cgtcactccg tgcgagagga tcccctcgag
     1021 cgctcccttg gcgtaggcgc cgaggacccc atcgcgacgc cagacgtgcg tcagggtgcg
     1081 cgtgacggga tcgaactcat ggtcgcggac gagctcggcc gcgtggacgc gcgagacatc
     1141 gatcccatgc gtcgccgcgg cgcgcacgat ggcctgctcg agcggatcga agggctccgg
     1201 ttccgaggcg agcagcgcga gcgtgagcag gtcgcgctcg gtgacgcccg cgaccgccca
     1261 ctggtcggcg acctcgatcg tcccaagcgt gagcgtgccc gtcttgtccg cgcagatcac
     1321 gctcgtcgcg ccgagcgtct cgacacccac gagcctgcgc acgagggcgt ggtcccgcgc
     1381 cagccgccac gcgccgagtc ccagatagag cgtgaagacc atcgggaact cctcggggat
     1441 ggccgccatc gcgagcgaga cgcccgcgat gagcgcctcg gcggcgctgt gaccgcgcgc
     1501 gagctcgatg acgatgacgc cgacgcagaa cgccgccgcg acgacggaca gctgcacgaa
     1561 cagcttgcgg atcacctgct gcagcggcgt cggcgagggc gcgatccgcg cgacgagctc
     1621 gccgatcgtg ccgtagcggg tcttcgcgcc ggtgaccgcg atcaccgcgg tcgcgcgtcc
     1681 ggcgagcacg gtcgtgccgg cccacagcgc ggcctcctcc tcgttgagca cggtcttggt
     1741 cagggcctgc gactcgccgg tgagcgccga ttcgtcgacg acgagctggg cgccggagac
     1801 gagccgcgca tcggcgggaa tgacgtcgcc ctcccgcagg acgatggcgt cacccgggac
     1861 gaggtcgagc gcggggacga gctcctcgcg gccgtcacgg atgacgcgcg cgagcggtgc
     1921 ggtgagcctg cgcaggctct cgagcgcatg ctccgcgcgc agcccgagca cgagaccgac
     1981 accgacgatc ggggccagcg cgacgagcgc gatcacgccg tcgcgcgtct cgccgagcgc
     2041 gaagtaggtc accgcggcga cgacgaggag gatcgccatg gggtcggtga cgatgtgccg
     2101 cagggtggcc cagagtcccg tcgcctcgtg ctccggtacg agcaggttgg gaccgaagac
     2161 gtcgaggcgg cgacgcgcct cgaccgcggt gaggccgagc gcctcggtca ccgctacggt
     2221 tcgagcgccg gtgtcctccg agagagccct gacgcgccgg tcgccgcggc gacggcgtgc
     2281 gcgacctggg ggacgacctc gcggtcgaac atcgacggca ggatgtactc ggccgagcgc
     2341 aggtccgccg gcaccgtcgc cgcgagggcg gccgcggccg cgagctcgat gtcgggcgac
     2401 acgccacggg cgtgcgcgtc gagcagtccg cggaacacgc cggggaacac gagcgagttg
     2461 ttcacctggt tggggtaatc gctccgtccg gtcgcgagga cgtctacctc cgacgcggcg
     2521 accagcggat cgatctccgg gtcgggattg gcgagcgcga agacgatgcg cggccgcgcc
     2581 atcgtgcgca gcgcatcgat cgtcaggacg cgcggaccgg agagcccgat gaagacgttc
     2641 gcgccggcga cggcatcctc cagtgtgccg gcccgctgcg tgccgagctc tcgcgcgatc
     2701 gcgaccttct cggcgttctg gtcgctgcgc gccgcgttca cgatcccgtg ccggtcgcag
     2761 aggacgatct cgcgcacgcc ggcgaggtgg agcaggcgcg cggtcgcgat acccgccgcg
     2821 ccggcaccgg cgatgacgac cctcaggtca cggatctcgc gctggacgac cgcggccgcg
     2881 ttgcgcaggg ccgcgagcac gacgaccgcg gtgccgtgct gatcgtcgtg gaagaccggg
     2941 atgtcgaggg tctcgcgcag cttgcgctcg acgatgaagc acgacggcgc cgcgatgtcc
     3001 tcgagattga tcccgccgaa gaccggcgcg acgtgctgca ctgtgcgcac gatctcgtcc
     3061 ggatccttcg tggcgaggca gagcgggaac gcggtgatgc cggcgaactc cgagaagatg
     3121 agggcctttc cctccatcac cggcatcgcc gcctcgggac cgatgtcgcc cagcccgagc
     3181 acggccgtcc cgtccgtcac gactgccacc gtgttctgcc ggatcgtcag cttccagacg
     3241 ctctgctggt cctccgcgat cgcgcggcag atgcgcgcga caccgggcgt gtacacgagg
     3301 gacagatcgt cgcgcgtccg gacctggacg cgccccttga tctcgatctt cccgccgagg
     3361 tggagcagga acgcccggtc ggagacggaa cggaccctca cgccgtcgag agcctggagg
     3421 tcgtgcagga gcttctggcc tgtctgctcg tcggggcagt ccacggtgac gtcgcggacg
     3481 tgcacggccg aggtggcctc gacgaggtcg atcgcgccca ggctgcaccc gcgggcgccg
     3541 atggtcaggg cgacgtgcgc gaacatgccg ggccgattgg cgatctcgag gcggaagacg
     3601 aggcgatacg ccggcgtgag gacagcgtgt ggctgcacct cggtcagtat ggccacgaag
     3661 gcgacgctag gcacacgggc cgaggcgagc gagggccgtt cggcagcccg cgacgtatgc
     3721 cgttcggcac ttggacgccc gtccggcggg cgcagattac cggcatggtc tcgatggatc
     3781 ggcaaccggt gtcccccaag acgcgcctcg tccagtgccc gctgcgcgac cgacaggtcg
     3841 ccatcgaaaa gtgcctgcaa tgcgggcgcc tgatcgacaa ggacagcgaa gttcccccgc
     3901 gctacgtcgt gtgcgacgcc cgcgtcgtca ccggctggct cggcctcgac gatctctagc
     3961 taagagcgct tcgctcgctt aagggctcgc tcgtcgcagc aaagctgctc ctcactcgtt
     4021 cctttggaca tgcggtcggc cctcatcctg atcctgagcc tgatcggcat cgctcccgcc
     4081 gcgcccggga ccgccgcgcc ggcgaccgcc acggccacgt gcacatcgtc ggtgggaccg
     4141 ggcatcccgc cccccgcgcg cgtgcctggc ggcatccccg gcttccacgc cgcgtggtac
     4201 ggccaatccg gctacatgcg gctctgcccg ggcgagcgat cgaccgcgac cgtcgcgtac
     4261 tacaacagcg gttcgcgcgg ctgggtctcg ggtcgcatgg gcgaggtcgc gtacctcgga
     4321 tcgtgggaca gcgagccggg tcaggaccag ccgagcgtcc tcggcggcga cgggcagctc
     4381 ggcagtccga acaccgcgtg gccacgcttc aaccgggccg cgctgcagcc cgcaccctac
     4441 gtcggccccg gtcaggtcgc gtggttccaa ttctccgtcc aggccccggc gcgccccggc
     4501 acctaccgcc tcggcatccg tccgctcgtt gagggcgcgc agtggatgga ggactacggc
     4561 gtgtactggg agatcaccgt cctgaatccc gacggcacca cgccgctcgc cacaccgcca
     4621 tcgccggcgg ggttctatta cgcgaccggg gccggcgtcg gcgccatgga catcgccgac
     4681 gtgcacgagg gcgtcgaatg gatctcgagc tatctcgcgg cgaacgccgg cggcgatcgc
     4741 cgtcgcatcg cgaccgcgcg gatcgccacg ggcgacggca cggagcagta ctgctgcatc
     4801 acccaggggc cgacgttcga catcgtgacg agcaatcgcg cgtggagctc accgcccgcg
     4861 gcggcggccg acacgtggac acccgacacc gagcgcatcg agctcgcggc gcacgagtac
     4921 gtgcacgtgt ggcaatacga caccggcggg cgcgcgtgca tggtcggcgg tccgcgctgg
     4981 atctcggaag gcatggccga gtcgctcgcg taccgctcgc tggtggccgc gggacggatc
     5041 ccggccgcga acatggacac cttcacgaag cggcagctgc ggagcgcgca atacgtgacg
     5101 ctgcgctcgc tggaatcgac ctggccgtcg gccgcgaacc cgttcgccgt cgcgtacctc
     5161 gcggtggacc ggctgctcgc gcagccgagc ccgctcgcgc tgcgcaccta ctgcgcgcgg
     5221 gtcggcgccg gcgagctgtg gcagacggcg ttcgccgcgg ccttcggcac cgacgccgac
     5281 accttctaca cgcgcttcga ggcgtaccgc acgcagtacc tcgccacgcc ctgagctacg
     5341 ggtagcgcgg cgcgccgggt gcgttggtcg ccggcgcggc gctcgctggc gggttggtca
     5401 tgtacggggt tgcg
//
LOCUS       DASTZR010000322         2182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1141445, whole genome shotgun sequence.
ACCESSION   DASTZR010000322 DASTZR010000000
VERSION     DASTZR010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141445"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..655
                     /locus_tag="VFV20_07190"
     CDS             101..655
                     /locus_tag="VFV20_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288169.1"
                     /translation="MEDLEQDRHLGPAEAAEVCDFLCMHGYPLYAAWARSRTDPVLPA
                     FLTEVTRWLGGKDVFFEEFGMPARTHEDEHTAEIFVAEALDGLHDAGATGAMLWCYAD
                     YPEAIWDRPPLDVAPHERYFGLFRADGTAKPAARHLGRFGGAARRPARSAPWTDADPE
                     RFYDAPLENLRTLYLRYTTAEVSR"
     gene            652..933
                     /locus_tag="VFV20_07195"
     CDS             652..933
                     /locus_tag="VFV20_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288170.1"
                     /translation="MSADVRARVLAEVAERVALLNEFRTDDFQDQVARLVSGAREHWR
                     EGHELPARGADPMLDALLGMSDMADSSPIAVRDACATALVAAGDARVTR"
     gene            complement(949..1995)
                     /locus_tag="VFV20_07200"
     CDS             complement(949..1995)
                     /locus_tag="VFV20_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288171.1"
                     /translation="MLEIGALFAYLGRQIGRLVQLAFNWATLVLFGQVAKEKQLLLSI
                     MAALSLLWPVAVAGVVLPQVGTFVLGLVTVPERFEPWVRLVMLAVALIAPLGVGVLAV
                     KLRDQAPKGLRLVGSLFAGYPNAMALAVVLLWLMVVAPITKLLAIARRHESAHVPIAV
                     KPGGYDTVVDDLRAALERAGLEVRHTFAPWPLTLPGRVLALAGGAGVRALVPTHLSQL
                     RAKDFVMTIHPMDLTLSGKTRALGRARAALVRELTFTQAYQTWTMEAHAIEDDIERAA
                     QGGELAPVGARLDAADVPFEEWEILYRMLLQTRLRRARVIGGDEVRVRTKENGQVGLW
                     ERIEAAVAALTARP"
BASE COUNT          343 a          767 c          785 g          287 t
ORIGIN      
        1 cgttccagcc actcgcgatc aagcccgcgc ttggctggcg aacatcaacg ccgttttacg
       61 ttccgccgat ccggcatgcc gcgtgactgt cggactgcat atggaagatc tggagcagga
      121 ccgccatctg ggaccggcgg aggccgcgga ggtctgcgac ttcctctgca tgcacggcta
      181 cccgctctat gcggcgtggg cgcgcagccg caccgatccc gtgctgccgg ccttcctcac
      241 cgaggtgacg cggtggctcg gtggcaagga cgtcttcttc gaggagttcg gaatgcccgc
      301 gcgcacgcat gaggacgagc acaccgccga gatcttcgtc gcggaggcgc tcgacgggct
      361 gcacgatgcc ggtgcgaccg gcgcgatgct gtggtgctac gcggactacc cggaggcgat
      421 ctgggatcgt ccgccgctcg acgtcgcgcc gcacgaacgg tacttcgggc tgttccgcgc
      481 cgacgggacc gcaaagccgg cggcgcggca cctcggtcgc ttcggcggcg cggcgcgccg
      541 tcccgcgcgg agcgccccat ggaccgacgc cgacccggag cgcttctacg atgcgccgct
      601 cgagaacctg cggacgctct acttgcggta cacgaccgcc gaggtctcac gatgagcgcc
      661 gacgtgcgcg cgcgggtcct cgccgaggtg gcggagcgtg tcgcgctgct caacgaattc
      721 cgcaccgacg acttccagga tcaggtcgcg cgcctcgtga gcggcgcgcg cgagcactgg
      781 cgcgaggggc acgagcttcc cgcacgcggc gcggacccga tgctggacgc gctgcttggt
      841 atgagcgaca tggccgacag ctcgccgatc gcggtgcgcg acgcgtgcgc cacggcgctc
      901 gttgccgcag gagacgcgcg ggtcacgcgc tgacgctgaa ggaggtcgct acggtcgcgc
      961 ggtgagcgcg gcgacggcag cctcgatccg ctcccagagg cccacctggc cgttctcctt
     1021 ggtccgcacg cggacctcgt cgcccccgat gacgcgcgcg cgacgcaggc gcgtctgcag
     1081 cagcatgcgg tagaggatct cccattcctc gaacgggacg tccgccgcgt ccagacgcgc
     1141 gccgaccggc gcgagctcgc cgccctgcgc ggcccgctcg atgtcgtcct cgatggcgtg
     1201 cgcctccatc gtccaggtct ggtacgcctg cgtgaacgtg agctcgcgta cgagcgcggc
     1261 gcgcgcccgc cccagcgcgc gcgtcttgcc cgagagcgtg aggtccatcg gatggatcgt
     1321 catgacgaag tccttcgcgc gcagctgtga gaggtgggtc ggaacgagcg cgcgcacgcc
     1381 ggcgccgccg gccagggcga gcacccggcc gggaagggtg agcggccacg gggcgaacgt
     1441 gtggcgcacc tccaggccgg cgcgctcgag cgccgcgcgc aggtcgtcga cgaccgtgtc
     1501 gtagccgccc ggcttcaccg cgatcgggac atgcgccgat tcgtggcgac gcgcgatcgc
     1561 gagcagcttg gtgatcgggg cgacgaccat cagccagagg aggacgacgg cgagcgccat
     1621 cgcgttcgga tagccagcga aaagcgatcc gacgagccgg aggcccttcg gcgcctggtc
     1681 gcgcagcttc acggcgagga cacccacgcc gagcggcgcg atgagcgcca cggcgagcat
     1741 cacaaggcgc acccacggct cgaaccgttc ggggaccgtg acgagcccga gcacgaatgt
     1801 accgacctgc ggcagcacca cacccgcgac cgcgaccggc cagagcagcg agagcgccgc
     1861 catgatcgaa aggagcagct gcttctcctt tgcgacctgt ccgaagagca cgagcgtcgc
     1921 ccagttgaac gcgagctgca cgaggcggcc gatctgccgg ccgaggtacg cgaagagggc
     1981 gccgatctcc agcacgagcg ggcgagcgtg caagagacgg gccgacgggc gatgatgcgg
     2041 agcggcatgg accgccgctt cctcgacagc ctcgccgagc aggtcgacca gatccgcgag
     2101 agcgggctgt acaagaagga gcgcgtcatc acctcgcccc agggcgccga gatccgcgtg
     2161 cgcccggacg cggaggtcgt ga
//
LOCUS       DASTZR010000323         3434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_823770, whole genome shotgun sequence.
ACCESSION   DASTZR010000323 DASTZR010000000
VERSION     DASTZR010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823770"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VFV20_07205"
     CDS             complement(<1..266)
                     /locus_tag="VFV20_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015696313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU5288172.1"
                     /translation="MTLTAALVRDRVRGTILERIGGTPLLRLRRIGAGLPGELWVKLE
                     SANPGGSVKDRAGLWMVLAAEESGRVGPDSILIDATSGNTGVALA"
     gene            complement(308..1591)
                     /locus_tag="VFV20_07210"
     CDS             complement(308..1591)
                     /locus_tag="VFV20_07210"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5288173.1"
                     /translation="MAQQVRSAMVADVGARIRSLRTAKGLTQAQLAEPLYTKAYISML
                     ESGRTRASMKALEHIAGVLGVKPSDLLGGSSAPSTPQFQFIEARRLLEEGRARDALPI
                     LEGLEEGLNAADQLVRLRYLAVAYNITAQPKRAFPVIERAQRMADLLESPEERVRVKA
                     VLANAHALTYAYDEAVRLLKECITACETGVVKDPAFHFRRLRELAVALTNQRDSRQAL
                     AAYEKALALADQFGDRPSLAILYAGMAKTYQNEGDLEAAILYNQRSLQLYEELGLLEQ
                     VACTLDNAAMLYAEYGNRERARECLTRAATIAQDTKRDGTIASIRASEAEVLAMTDPE
                     AGLAAAQEALKFARKVDQPDAQVRAMIVIGELRMASSAAAAKRSFQEASQLAEQRVPH
                     LLRVIFDRWSHAAEARGDSDEALRLARRALETVRT"
     gene            complement(1751..1876)
                     /locus_tag="VFV20_07215"
     CDS             complement(1751..1876)
                     /locus_tag="VFV20_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288174.1"
                     /translation="MSIRRMFQIVTLALSLVFAFGATGVSNVAYGCDPQTGGGLC"
     gene            complement(1966..2931)
                     /locus_tag="VFV20_07220"
     CDS             complement(1966..2931)
                     /locus_tag="VFV20_07220"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEU5288175.1"
                     /translation="MRADRAFGLVLALALVSSTVLGALLAAIVPRAEHVLSAGPDDLV
                     DAVTLLLLILATCGIALGLGSLLRQVLATMALIRALVARRVPTPETVRSLAREVGVEG
                     RVDVVADERPFSFCYWFWRPRLCISTALAQRLEPDEMRAVLVHERYHLRHRDPLRVLT
                     ARYLAAGLYVVPVVDELSSFYVLQKEIAADDEAVRIVGVGALARALYKLLPGAPDREL
                     GLLVPVTGLSVTEARIDRLAAGRDIPLRLSPLSIALSSGALVAAAVLASIRAGSSVAF
                     ADLPPLLAVPGLLVGPVSLLFVAALNGGAHQVRALAGLVSRSRES"
     gene            complement(2921..3334)
                     /locus_tag="VFV20_07225"
     CDS             complement(2921..3334)
                     /locus_tag="VFV20_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEU5288176.1"
                     /translation="MSTPLRRIRRAATGDLLGPLESAVMERLWKRGPATVRDVVEDLG
                     RSRDLAYTTVMTVMVRLQGKGLLERERSGKTYVYRPVRTRREHEVRVSRDLARGLVSQ
                     FGDAALAAFSAELDEVDADHRAALRRIAKGPRDAR"
BASE COUNT          568 a         1179 c         1216 g          471 t
ORIGIN      
        1 gcgagcgcga cgcccgtgtt cccgctcgtc gcgtcgatga ggatgctgtc cggaccgacg
       61 cggcccgact cctcggccgc cagcaccatc cacaggccgg cgcgatcttt cacgctgccg
      121 cccggattcg cggactcgag cttcacccag agctcgccgg ggaggcccgc accgatgcgc
      181 cgcaggcgga ggaggggcgt tccgccgatg cgctccagga tcgtgccgcg cacgcgatca
      241 cggacgaggg ccgcggtaag ggtcaccgat cgaatctaaa ccgagcggcg cgctggcccg
      301 gcgacggtca ggtgcggacg gtctccagcg cacggcgcgc gagacgaagt gcctcgtcac
      361 tgtcgccacg cgcctcggcg gcgtgcgacc agcggtcgaa gatcacgcgg agaagatgcg
      421 gcacgcgctg ctcggcgagc tggctcgctt cctggaacga gcgcttcgcc gcggcggcgc
      481 tcgaggccat ccgcagctca ccgatgacga tcatcgctcg gacctgcgcg tccggctgat
      541 cgaccttgcg cgcgaacttg agcgcctcct gtgcggcggc gagacccgcc tcgggatcgg
      601 tcatcgcgag gacctcggcc tcggaggcac ggatcgatgc gatcgtgccg tcgcgcttgg
      661 tgtcctgcgc gatcgtggcg gcgcgcgtga ggcactctcg cgcgcgctcg cggtttccgt
      721 actccgcgta cagcatcgcg gcgttatcga gcgtgcaggc gacttgctcc agcaagccga
      781 gctcctcgta gagctgcagg ctccgctggt tgtagaggat cgcggcctcg aggtcaccct
      841 cgttctggta ggtcttcgcc atgcccgcgt agaggatcgc cagtgagggc cgatccccga
      901 actggtccgc gagtgccaac gccttctcgt acgcggcgag cgcctgacga ctgtcgcgct
      961 gattcgtcag cgccacggcg agctctcgga gtctccggaa gtggaaggcc ggatccttta
     1021 ccaccccggt ctcgcacgcg gtgatgcact ccttcaggag gcggacggct tcgtcgtagg
     1081 cgtaggtcaa agcatgcgca ttcgcgagca ccgcctttac gcgcacgcgc tcctcggggc
     1141 tctccagcag gtcggccatc cgttgtgcgc gctcgatcac cgggaacgcg cgcttcggct
     1201 gagccgtgat gttgtaggcg accgcgagat agcgcaggcg caccagctga tcagcggcgt
     1261 tgagaccttc ttcgagccct tcgaggatcg gcagtgcgtc gcgggcgcga ccctcctcca
     1321 gcagccggcg cgcttcgatg aactggaact gaggcgtcga aggagccgaa ctgccaccga
     1381 gaaggtcgga cggcttcaca ccgaggactc cggcaatgtg ctcgagcgcc ttcatcgatg
     1441 cacgcgtgcg accggactcc agcatcgaga tgtacgcctt cgtgtagagc ggctcggcaa
     1501 gttgcgcctg cgtcaggccc ttcgcagtcc gcagtgagcg gatgcgtgcc ccaacgtcgg
     1561 ccaccatcgc ggaccggacc tgctgtgcca tcggtctggc actttacggc gcttcaagtc
     1621 gctggtcaat ttggcctgaa ctctcagatt cgggaccgag accggagcga tgccggtcgg
     1681 ggtccacggg cctagcgtgc ccgggtatct cggtcggtgc ggcgagaagc acgcaccagg
     1741 atcgcggtcg ctagcagagg ccgccgccgg tctgcgggtc gcagccgtac gcgacgttcg
     1801 acacaccggt agcgccgaag gcgaaaacga gcgagagagc gagggtgacg atctggaaca
     1861 tacgacggat ggacattcgg tgggcctccc tgaccccacc ggcgatttct tacccaccag
     1921 gcccgtcgcg gccgactatc ggttacagcg tggcggaagt aaaggtcaac tttcccgcga
     1981 acgactgacc aaacccgcga gagcccggac ctgatgagcg ccaccgttca gggccgcgac
     2041 gaagagcagc gacacggggc cgacgagcag gccagggacg gcaagcagcg ggggcaggtc
     2101 ggcgaaagcc acgctcgagc cggcacggat cgaggcgagc acggccgcgg caacgagcgc
     2161 acccgacgac agcgcgatcg agagcgggga caggcgcagc gggatgtcgc ggccggcggc
     2221 gagacggtcg atccgagcct cggtcaccga caggccggtg acgggcacga gcaggccgag
     2281 ctcgcgatcc ggcgcgccgg ggaggagctt gtagagggca cgcgcgagcg cgcccacgcc
     2341 gacgatccga acggcctcgt cgtcggcggc gatctccttc tgcaggacgt agaacgacga
     2401 gagctcgtcc accaccggca cgacgtagag gccagcggcg agatagcggg ccgtcaggac
     2461 ccgcagcgga tcgcggtgcc gcaggtggta gcgctcgtgg acgagcacgg cacgcatctc
     2521 atccggttcg agccgctgcg ccagcgcggt cgagatgcag agccggggcc gccagaacca
     2581 gtaacagaag ctgaacggac gctcgtcggc gacgacgtcg acgcggccct ccacgccgac
     2641 ctcgcgcgcg agcgaccgca ccgtctcggg cgtgggcaca cgtcgcgcca cgagcgcgcg
     2701 gatgagggcc atcgtcgcga ggacctggcg cagcagcgag ccgaggccga gcgcgatccc
     2761 gcacgtcgcc aggatgagga gcagcagcgt gaccgcgtcg acgaggtcgt cgggcccggc
     2821 ggagaggacg tgctccgcac gcggaacgat cgcggccagc agcgcgccga gcacggtgct
     2881 cgacacgagg gccagcgcga gcacgagacc gaaggctcgg tcagcgcgca tcacgcggtc
     2941 ccttcgcgat gcggcgcagc gccgcgcgat ggtcggcatc gacttcgtcg agctcggcgc
     3001 tgaatgcggc gagcgcggcg tcgccgaatt gggagacgag gccgcgcgcg agatcgcggg
     3061 acacacgcac ctcgtgctcc cggcgcgtgc ggaccggacg gtacacgtac gtcttcccgc
     3121 tgcgctcgcg ctcgaggaga cccttcccct ggagacggac catcacggtc atcacggtcg
     3181 tatacgcgag atcgcgcgag cgcccgagat cctcgacgac gtcgcgcacc gtggccggcc
     3241 cgcgcttcca gagccgctcc atgaccgccg actcgagcgg cccgagcagg tcccccgtcg
     3301 cggcgcgccg tatccggcga agcggcgtgc tcacgcgacg atcatcccac cttgcgggtg
     3361 cggcccaaac ggcggcgggt cagcatgcgc gggtgccgct cggcgatgcc ctcgcggccg
     3421 tgcgccaggg ccac
//
LOCUS       DASTZR010000324         6973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_149178, whole genome shotgun sequence.
ACCESSION   DASTZR010000324 DASTZR010000000
VERSION     DASTZR010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6973
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149178"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VFV20_07230"
     CDS             <1..225
                     /locus_tag="VFV20_07230"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEU5288177.1"
                     /translation="PPAGSVGSDAETKIDLNHATAAELEALPGIGPATAGRIVRSRDA
                     RPFGKVDELQTRGLVTARVLADIRDLLTLR"
     gene            222..2327
                     /locus_tag="VFV20_07235"
     CDS             222..2327
                     /locus_tag="VFV20_07235"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEU5288178.1"
                     /translation="MSALLSIPLLPATTAAALGAMAFGAGAIVGVMVTTLLAAAGALL
                     ALASLGRRRLVWWCATAAAVAVGAHALVPISLVPPGLLAFDVPRAALARPIELLVPSP
                     ESALLLGIVLGERGSVPADLSLAFARSGTTHLLAISGFNMTLVATAVSLALRGRAAPP
                     VRAAVAVSAIVLYSLLVGLSPSVLRAALMAAVAAVGVVSGRRAATANGLCAAIVAMLV
                     ADPSAVSDVGLQLSALATAGLVLWQSPVASRLDRLPGALREGIATTVAATLPTIPVVA
                     ATFGRVSVVSIVANLVCVPLFPPLMLAGAATSLAGAASLDAARPVALLAFGVAAALRG
                     AVTTFASLPLAALDVPSGPLTAVAVTGLEVAAVWCVRGLLHRAKVAGGVRRGTLLGQA
                     LRSGTLPQLAPPRWAARRGLAHGLVVAAAAAPAALLVAWPSPPPVARVTALDIGQGDA
                     YLVEAGDAVMLVDGGPDPARLLDELGATLGPWRRRIDVVALTHAHTDHGAGLLAVLDR
                     YEVGLAIEPRGLNPGPLTDLWSKAIASRGVVRRAVQMGERFSIGDLRVTVLAPDPDLR
                     VDVPSLVLHVERGAFSVLFTGDAVDDALARLLEHPRALRSRVYVPPHHGAETPHAAAL
                     RLAAHPDVALISVGAGNRYGHPTAPTLAALAGLALYRTDHDGSVEISLDGTGLVARTH
                     ANGLPPPRRGSIPYSAPRQ"
     gene            2332..3345
                     /gene="holA"
                     /locus_tag="VFV20_07240"
     CDS             2332..3345
                     /gene="holA"
                     /locus_tag="VFV20_07240"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU5288179.1"
                     /translation="MLSLVAGGRGERGDLATRRIEPLGAALGLTRVDARGSSPDDIIV
                     AGQSQGLFAAPDEQRVVMVEHAEALARTDVVARFPEDAALVLVAPEPMRSTRARGRKP
                     KAQSADLALLDAVENVGGTIERYDRLLAGDAARWVAARAQLYGVTLRPDALDALSSAL
                     AGDAERIENEVQKLAAWAGGAPVTAPDVRSLVPGAIESDVFELTKAVVRRDVRTAAPL
                     LSHLLDEGNAPQQLLALLLWQFRVLLFAASDPKPAEAERMAKAIRSSSGAIFRYTQFA
                     RGLGRKDIARAYEALYACDVSIKTGRAESDETALLLCVMDLCGVPGADLRDLLLAEAP
                     RRG"
     gene            complement(3568..4236)
                     /gene="fsa"
                     /locus_tag="VFV20_07245"
     CDS             complement(3568..4236)
                     /gene="fsa"
                     /locus_tag="VFV20_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEU5288180.1"
                     /translation="MKLFLDTAIYEEIEQGVSWGVVDGVTTNPTLIAKAGQDHEQQVK
                     KICQIIGNVSAEVVSEGRDDMIVEGRRLAAWHPNVIVKVPMTPDGLAAGKVLAGEGVR
                     INVTLCFSVNQALLAAAIGAYIISPFAGRLDDINEDGMKVVADIVSAYRQQEIKTKVL
                     AASIRNPMHVTQAALAGADIATMPFAVLTQMFNHPLTDAGQKKFLEDYRKTQEAKPVA
                     AAKR"
     gene            complement(4233..5888)
                     /locus_tag="VFV20_07250"
     CDS             complement(4233..5888)
                     /locus_tag="VFV20_07250"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU5288181.1"
                     /translation="MPKFVFVTGGVTSSLGKGITAASLGRILSARGLAVASLKMDPYL
                     NVDPGTMSPYQHGEVFVTEDGAESDLDLGHYERFIDRNVTRASNLTTGQIYNAVIAKE
                     RRGDYLGATVQVIPHVTNEIKERIHRVAREQQAEIVVVEVGGTVGDIESLPYLEAIRQ
                     FRNDVGRQNVLYVHLSLLPRVATGELKTKPTQHSVRELRAIGIQPDVLIARADEPIDE
                     ELREKIALFCDVKVDNVISEPNAWSIYAVPLMLEEAGLGRIVVEELGLKERAAEPDLA
                     EWRALTERIRNARIPLRIGLVGKYIELTDAYLSVAESVKHASWAAGCDVKISWIDSER
                     LERDRDVEPLIGLDGIVVPGGFGYRGIEGKLQAVRYGRERRVPTLGLCMGMQCMVIEL
                     ARTALGTEDANSTEFDAFTPHPVIDLLPEQRDVADKGGTMRLGSYPCVLEAGSLASRA
                     YGQPIVLERHRHRYEFNNAYRELLAQHGLRFSGLSPDGRLVEIVEIDDHPFFLGTQFH
                     PEFKSRPNRPHPLFASFIDASVQYARANGRAAAPAPMLQESNS"
     gene            complement(5944..>6973)
                     /locus_tag="VFV20_07255"
     CDS             complement(5944..>6973)
                     /locus_tag="VFV20_07255"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288182.1"
                     /translation="LLDVGDAHAALPRDLAPMRARQAAGAFNSSDYIFELKWDGLRAF
                     ASREADAVRLVDRNGGDLLPLVPELARLPIPEGSLLDGEIVVSDSRGRPSYDLMAGRL
                     GPKAAKRGFGPTFVAFDLLYLDRRPLLGRPLMERRRRLADLGLRGRAIGVPAHLEADG
                     EPFLEVVEEYGLEGIVAKRKTSAYVPGTRTSDWLKCHVTPRADVVIGGLVQDEPRGTV
                     RVLCGMYHDERDVLAMVGEAYVPPYLARWLDDATRDFASDVSPFSTPVPLRTGTRWLR
                     PKLVAIVEHAGETGALRDARFRALRLDGRLDDCRVDEPIVIESEPATMGTERPRLVVL
                     ESLRLNPM"
BASE COUNT          919 a         2497 c         2478 g         1079 t
ORIGIN      
        1 ccaccggccg ggagcgtggg ctccgacgcc gagacgaaga tcgatctcaa ccacgcgacc
       61 gccgccgagc tcgaggcgtt gccgggtatc gggcccgcga cggctggccg catcgtccgt
      121 tcgcgtgacg cgcggccgtt cggcaaggtg gacgagctgc agacgagggg tctcgtcacg
      181 gcccgcgtgc tcgcggacat ccgcgacctg ctcacgctgc ggtgagcgcc ctcctcagca
      241 tcccgctcct tcccgccacg accgcggccg cgcttggtgc gatggcgttc ggcgccggcg
      301 cgatcgtcgg cgtcatggtc acgacgctgc tggccgcggc gggcgccctc ctcgcgctcg
      361 cgagtctggg tcgccgccgc ctggtgtggt ggtgcgcgac cgccgccgcc gtcgcggtgg
      421 gcgcgcacgc gctcgttccg atctccctcg tgccgcccgg cctcctcgcc ttcgacgtcc
      481 cgcgcgccgc gctcgcgcga cccatcgagc tcctcgtccc gtcgcccgag agcgcgctgc
      541 tcctcggcat cgtgctgggc gagcgcggct cggtgccggc cgacctctcc ctcgcgttcg
      601 cgcggagcgg gacgacgcat ctgctcgcga tcagcggctt caacatgacg ctcgtcgcca
      661 ccgcggtgtc gctggcgctc cgcgggcgcg ccgcgccgcc ggtgcgcgcg gcggtcgcgg
      721 tgagcgcgat cgtcctgtac agcctgctcg tgggcctctc gccgagcgtg ctgcgcgcgg
      781 cgctcatggc cgccgtcgcc gcggtcggcg tcgtctcggg ccggcgcgcc gcgaccgcga
      841 acggcctgtg cgcggcgatc gtcgcgatgc tcgtggccga tccgtccgcc gtcagcgacg
      901 tcggcctgca gctgtccgcg ctggcgacgg ccgggctcgt gctctggcag tcaccggtcg
      961 cgtcgcggct ggaccggctt ccgggcgccc tgcgggaagg catcgcgacg accgtcgcgg
     1021 cgaccctgcc gaccatcccg gtggtcgcgg ccacgttcgg ccgcgtgtcg gtcgtgtcga
     1081 tcgtggcgaa tttggtctgc gtcccgctct ttccgccgct catgctcgcc ggcgcggcga
     1141 cgagcctcgc cggcgccgcc tcgctggacg ccgcacgtcc cgtcgcgctg ctcgcgttcg
     1201 gcgtcgccgc cgcgcttcgc ggcgcggtca cgaccttcgc gtcacttccg ctcgcggcgc
     1261 tcgacgtgcc gagcggacca ctcacggccg tcgcggtgac cgggctcgag gtcgcggcgg
     1321 tgtggtgcgt tcgcggtctt ctccaccgcg cgaaggtcgc gggtggcgtg cggcggggca
     1381 cgctgctcgg acaggcgctg cgatccggga ccctgcctca gctcgcgcct ccgcggtggg
     1441 ccgcgcgccg cggtctcgcg cacgggctcg tcgtggccgc cgcggcggca cccgccgcac
     1501 tcctcgtcgc gtggccgtcg ccgccgccgg tggcgcgggt caccgcgctc gacatcgggc
     1561 agggcgacgc ctacctcgtg gaagcgggcg acgcggtgat gctggtcgac ggcggacctg
     1621 atcccgcgcg gctgctcgac gagctcggcg cgacgctcgg accgtggcgc cggcgcatcg
     1681 atgtcgtggc gctgacgcac gcacacaccg accatggagc ggggctgctc gcggtgctgg
     1741 accgttacga ggtcggcctg gccatcgagc cgcggggtct gaaccccggt ccgctcaccg
     1801 atctctggtc gaaggcgatc gcgtcacgcg gcgtcgtgcg gcgtgcggtc cagatggggg
     1861 agcgtttctc catcggcgat ctgcgggtga ccgtgctcgc gcccgatccc gacctgcgcg
     1921 tcgacgtacc gtcgctcgtg cttcacgtcg agcgcggtgc gttctcggtg ctgttcaccg
     1981 gcgacgccgt ggacgacgcg ctcgcgcgcc tgctcgagca cccgcgggcg ctgcgttcgc
     2041 gggtgtacgt gcctccgcac cacggcgcgg agacgccaca cgccgcggcg ctccgcctcg
     2101 cggcccatcc ggacgtcgcg ctcatctccg tcggggcggg gaaccggtat ggtcatccca
     2161 ccgcgccgac gctcgcggcg cttgcgggcc tcgcgctgta ccgcaccgat cacgacggga
     2221 gtgtggagat ctcgctcgat ggcacaggcc tcgtcgccag gacccacgcg aatggtcttc
     2281 ctcctccacg gagaggatcc attccgtatt cggctccgcg ccaatgagct cgtgctctcg
     2341 ctcgtcgcgg gcggccgcgg cgagcgtggt gacctcgcga cacgccggat cgagccgctc
     2401 ggcgcggctc tcggcctcac gcgcgtcgac gcccgcggga gcagtcccga cgacatcatc
     2461 gtggcgggcc agtcgcaggg actcttcgcc gcacccgacg agcagcgcgt cgtgatggtg
     2521 gagcacgccg aggcgctcgc gcgcaccgac gtcgtcgcgc ggttccccga agacgcggcc
     2581 ctcgtgctcg tcgcgcccga gccgatgcgg tcgacgcgag cgcgcggacg gaagccgaag
     2641 gcgcagagcg cggatctcgc gctgctggac gcggtcgaga acgtgggagg cacgatcgag
     2701 cgatacgacc ggctcctcgc cggcgacgcg gcccgctggg tcgccgcgcg cgcgcagctc
     2761 tacggcgtga cgctccggcc ggacgcgctc gacgcgctct cctcggcgct cgcgggcgac
     2821 gccgagcgga tcgagaacga ggtgcaaaag ctcgccgcgt gggcgggcgg cgcgccggtc
     2881 accgctcccg acgtccgctc gctggtcccc ggcgcgatcg agtccgatgt gttcgagctc
     2941 accaaggcgg tcgtgcgccg cgacgtgcgc accgcggcgc cactcctgag ccatctcctc
     3001 gacgagggca acgcccccca gcagctgctg gcgctgctcc tgtggcagtt ccgcgtgctc
     3061 ctcttcgcgg cgagcgatcc gaagccggcc gaggccgagc gcatggcgaa ggcgatccgc
     3121 tcctcgtccg gcgccatctt tcgctacacg cagttcgcgc gcggactcgg ccgaaaggac
     3181 atcgcgcgcg cgtacgaggc gctctacgcg tgcgacgtgt cgatcaagac cggtcgcgcg
     3241 gagtccgacg agaccgcgct gctgctctgc gtcatggatc tctgcggcgt ccccggcgcc
     3301 gacctccgtg atctcctgct cgccgaggcg ccgcgaaggg gttagcgccc ggtcgaatcc
     3361 gacgcgcgtg cggcgaacga cgcggagcga aggcggcagc gtagcggcgg gccgatgggt
     3421 actcgcgcga cgagcgcctg cgcccagcgc gcccgaccgt ttcgcgtatg ccacgtgagc
     3481 gggcgcgtag ccagcagcga ggagcgcgag tacccggaga gcccgccgcc gcggtgtggg
     3541 cgcgcgagcg cgacgcgcgc cggaggctta ccgcttcgcg gctgcgaccg gcttggcctc
     3601 ctgcgtcttc cggtaatcct cgaggaactt cttctggccc gcgtcggtga gcgggtggtt
     3661 gaacatctgc gtcagcaccg cgaagggcat cgtcgcgatg tcggcacccg cgagcgcagc
     3721 ctgcgtgacg tgcatcggat tgcggatcga cgcggcgagc accttcgtct tgatctcttg
     3781 ctgccggtac gccgagacga tgtccgcgac gaccttcatc ccgtcctcgt tgatgtcgtc
     3841 gagccgtccc gcgaagggac tgatgatgta cgcgccgatc gccgccgcga ggagcgcctg
     3901 gttcacggag aagcagagcg tcacgttgat gcgcacgccc tcaccggcga ggacctttcc
     3961 ggcggccagg ccgtccggcg tcatggggac cttcacgatc acgttcggat gccaggccgc
     4021 gaggcgccgg ccctcgacga tcatgtcgtc gcggccctcg ctcacgacct ccgcggagac
     4081 gttcccgatg atctggcaga tcttcttgac ctgctgctcg tgatcctgcc cggccttggc
     4141 gatgagcgtg gggttcgtgg tgacgccgtc gacgacgccc cagctcacgc cctgctcgat
     4201 ctcttcgtag atcgcggtgt cgaggaagag cttcatgagt tcgactcctg cagcatcggt
     4261 gccggtgcgg cggcacgacc gttggctcga gcgtactgga cgctcgcgtc gatgaaggag
     4321 gcgaacagcg gatgcgggcg gttgggacgt gacttgaact cgggatggaa ctgcgtgccg
     4381 aggaagaacg ggtgatcgtc gatctcgacg atctccacca ggcgtccgtc cggcgagagt
     4441 cccgagaaac gcaggccgtg ctgcgcgagc agctcgcggt aggcgttgtt gaactcgtag
     4501 cggtgacggt ggcgctccag cacgatcggc tggccgtatg cgcgagaggc cagcgaccca
     4561 gcctcgagca cacacggata gctgcccagt cgcatcgtgc cgcccttgtc ggcgacgtcg
     4621 cgctgctcgg gcaacaggtc gatgacgggg tgcggagtga acgcgtcgaa ttcggtcgaa
     4681 ttcgcgtcct ccgtacccag ggccgtgcgc gccagctcga tgaccatgca ctgcatcccc
     4741 atgcagaggc cgagcgtggg cacgcgtcgc tcgcgcccgt agcggaccgc ctgcagcttg
     4801 ccctcgatcc cgcggtagcc gaagccgccg ggcacgacga tgccatcgag cccgatgagc
     4861 ggctcgacgt cgcggtcgcg ctcgagacgc tcgctgtcga tccaggagat cttcacgtca
     4921 cagccggcgg cccaggaggc gtgcttcacg gattccgcga ccgagaggta cgcgtccgtg
     4981 agctcgatgt acttcccgac gagcccgatg cgcagtggga tccgcgcgtt ccggatgcgc
     5041 tcggtcagcg cgcgccactc cgcgaggtcc ggctccgcgg cgcgctcctt caggccgagc
     5101 tcctccacga cgatgcgtcc caggccggcc tcctcgagca tcagcggcac ggcgtagatc
     5161 gaccaggcgt tcggctcgga gatcacgttg tccaccttga cgtcgcagaa gagcgcgatc
     5221 ttctcgcgca gctcctcgtc gatgggctcg tccgcgcgcg cgatgaggac gtcgggctgg
     5281 ataccgatgg cgcgcagctc gcgcaccgag tgttgcgtgg gcttagtctt cagctcgccc
     5341 gtcgcgacgc gcggcagcag tgagaggtgg acgtacagca cgttctgccg gccgacgtcg
     5401 ttgcggaact ggcggatcgc ctcgaggtag ggaagcgact cgatgtcgcc gaccgtgcca
     5461 cccacctcga ccacgacgat ctcggcctgc tgctcccgcg cgacgcgatg gatgcgctcc
     5521 ttgatctcgt tggtcacgtg cgggatgacc tgcacggtcg cgccgagata gtcgccgcgg
     5581 cgctccttcg cgatgaccgc gttgtagatc tggccggtgg tgaggttgga ggcgcgcgtc
     5641 acgttccggt cgatgaagcg ctcgtagtga ccgaggtcga ggtcgctctc cgcgccgtcc
     5701 tcggtgacga acacctcgcc gtgctgatat ggcgacatcg ttccggggtc gacgttgagg
     5761 tacggatcca tcttgagcga ggcgacggcg agaccgcgcg cggaaaggat gcgaccgagc
     5821 gatgcggcgg tgatgccttt gccaagagag gaggtgaccc cgcccgtcac gaatacgaac
     5881 ttcggcaacg cgacgacctc cggggttttt ccaattgtac cccggtgacg cgacgcgcag
     5941 gaatcacatg ggattcaagc gaagactctc gaggaccacg aggcgcgggc gctcggtgcc
     6001 catcgtcgcg ggctcgctct cgatcacgat gggctcgtcg acgcggcaat cgtcgagcct
     6061 tccatcgagc cgcagcgcgc ggaatcgcgc atcgcggagc gcgcccgtct cgcccgcatg
     6121 ctcgacgatc gcgaccagct tcggccgcag ccatcgtgtg cccgtgcgca gcggtaccgg
     6181 cgtgctgaac ggcgacacgt cggacgcgaa gtcgcgcgtc gcatcgtcga gccaccgcgc
     6241 gaggtacggc ggaacgtacg cctcgccgac catcgcgagc acgtcgcgct catcgtgata
     6301 catgccgcag agcacacgca ccgtgccgcg gggctcatcc tgcacgagcc cgccgatcac
     6361 cacgtcggcg cgcggcgtca cgtggcactt cagccagtcg ctcgtgcgcg tgccgggaac
     6421 gtacgcgctc gtcttgcgct tcgcgacgat cccctcgagg ccgtactcct ccacgacctc
     6481 gaggaacggc tcgccgtcgg cctccagatg cgcggggacg ccgatggcgc gaccgcgcag
     6541 cccgaggtcc gcgaggcggc gccggcgctc catgagcgga cgcccgagca gcggacggcg
     6601 gtcgaggtag aggaggtcga atgccacgaa agtgggccca aatccgcgct ttgcggcctt
     6661 cggaccgagc cgcccggcca tcaggtcgta cgacggccgg ccgcgcgagt cagagaccac
     6721 gatctcgccg tcgagaagcg agccctccgg gatcggcagc cgcgcgagct cgggcaccag
     6781 gggcaggagg tcgccgccgt tccggtcgac gagccggacg gcgtcggcct cacgtgaagc
     6841 aaatgcgcgc aggccgtccc acttcagctc gaagatgtag tcgctggagt tgaacgcgcc
     6901 ggcggcctgg cgcgctcgca tcggagcgag gtcacgcggc aacgccgcgt gcgcatcgcc
     6961 gacgtcgagg agc
//
LOCUS       DASTZR010000325         2685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_96022, whole genome shotgun sequence.
ACCESSION   DASTZR010000325 DASTZR010000000
VERSION     DASTZR010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96022"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..538
                     /locus_tag="VFV20_07260"
     CDS             122..538
                     /locus_tag="VFV20_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003942513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEU5288183.1"
                     /translation="MFASAETARVAHLATADQYSRPHVVPIVFVLHGDTLYFPLDRKP
                     KRDDDWHMLRRVRNIETNGRVSIVIDRYDEDWSKLAWVLLEGVATILESGSERDEAAR
                     ALAEKYAQYAGSALDGRPVVRVTVERRVSWSAGQQT"
     gene            582..1595
                     /locus_tag="VFV20_07265"
     CDS             582..1595
                     /locus_tag="VFV20_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006135026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03842 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HEU5288184.1"
                     /translation="MEFGFTLKPDVSLERAAALARRAEACGFTYGWIFDSHVLWKEPY
                     VPLTLMAQATTTMRLGTCVTNPATREPSVTASTLATLQILSGGRFDLGIGRGDSARRV
                     IGKPPTTLAVLEETTKVIKDLAEGRSVTYEGTTVRLEWAAPYPLPVWIAGYGPKALEV
                     TARIADGAIIQLADPSLVEWCVGLLREGARSVGRDPNKIAVMAAAAAYVGPREIALER
                     VRWFPALVSNHVVDLVKRYGETSLPKDLTTYVKDRPGYDYLHHAEVGSSNAKFVDDES
                     VERFCVVGSVDDHIAKLRRLARVGVRQFNIYLMNRDEEEQLEIYGEKIIPALKDVETV
                     AIA"
     gene            1730..2134
                     /locus_tag="VFV20_07270"
     CDS             1730..2134
                     /locus_tag="VFV20_07270"
                     /inference="COORDINATES: protein motif:HMM:NF040320.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288185.1"
                     /translation="MNSPTSTIELVTDLSQDRPGEIAKAVTAIANMSINIEGFCEVDG
                     RLHLVTSDPENARKALETVGFVVEEREIFVIEADDKPGFLANILRRLSAEELNIIATY
                     TLTKTRLAFTVDQPARMRDILRDLSPTTTRVR"
     gene            complement(2206..>2685)
                     /locus_tag="VFV20_07275"
     CDS             complement(2206..>2685)
                     /locus_tag="VFV20_07275"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5288186.1"
                     /translation="IWATERLIAHCRTLSDAQLELTAPGTYGTIRRTLEHIVGADERY
                     LLRLGIATADPPFREDHDVPLDEIASHLALVQAGVENLFAEREFDPDRVVLDTRQRGP
                     TDPQLELEAWMMVTQFTHHGSDHRAHIGTILGAHGLAAPELDVWAYGRELGAIRERR"
BASE COUNT          427 a          917 c          945 g          396 t
ORIGIN      
        1 gcatgtgcag ggcccggacg atgagcgccg gcaggtcgtc accggggtgg atctcgggca
       61 ggccgtgcgc gggcgtgatg accaggcggc ggacgctcac cctttgcgta ccatcgcgct
      121 catgttcgca tccgcggaga ccgcgcgtgt ggcgcacctc gcgaccgccg accagtactc
      181 acgcccgcac gtcgtcccca tcgtgttcgt cctgcacggc gacaccctgt acttcccgct
      241 cgatcgcaag ccgaagcgcg acgacgactg gcacatgctg cggcgcgtcc gcaacatcga
      301 gacgaacggt cgcgtgtcga tcgtgatcga ccggtacgac gaggactggt cgaagctcgc
      361 ctgggtcctg ctcgaggggg tcgcgacgat cctcgagagc ggcagcgagc gcgacgaggc
      421 cgcacgcgcg ctcgcggaga agtacgcgca gtacgccggg agcgcgctcg acggtcgccc
      481 cgtcgtgcgc gtcaccgtgg agcggcgcgt cagctggagc gcaggccagc agacctgagc
      541 gctccggtct cgcggcggcc ggcgatatgc ttccgcgtct catggagttc ggtttcacgc
      601 tcaagccgga cgtctcgctt gagcgcgccg cggcgctcgc acggcgagcc gaggcgtgcg
      661 gcttcaccta cggctggatc ttcgacagcc acgtgctctg gaaggagccg tacgtcccgc
      721 tcaccctgat ggcgcaggcg acgacgacga tgcgcctcgg cacctgcgtc accaacccgg
      781 cgacccgcga gccgtcggtg accgcgagca cgctcgcgac gctgcagatc ctctccggcg
      841 gccgcttcga cctcggcatc ggccgcggcg actccgcacg gcgtgtgatc ggaaagccac
      901 ccacgacgct cgccgtcctc gaggagacga ccaaggtgat caaggacctc gcggaggggc
      961 gcagcgtcac gtacgagggg acgaccgtgc gcctcgagtg ggcggccccg tatccgctcc
     1021 cggtgtggat cgccggctac gggccgaagg cgctcgaggt gaccgcgcgg atcgccgacg
     1081 gcgcgatcat ccagctcgcc gacccaagcc tggtggagtg gtgcgtcggg ctgctgcgcg
     1141 agggcgctcg ctcggtgggt cgcgacccga acaagatcgc ggtcatggcc gcggccgccg
     1201 cctacgtcgg gccgcgcgag atcgcgctcg agcgcgtgcg ctggttcccg gcactcgtgt
     1261 cgaaccacgt cgtcgatctc gtgaagcgct acggcgagac gagcctgccc aaggacctca
     1321 ccacgtacgt gaaggaccgg ccgggctacg actacctcca ccacgccgag gtcgggtcct
     1381 ccaacgcgaa gttcgtggac gacgagtcgg tcgagcgttt ctgcgtcgtc gggagcgtgg
     1441 acgatcacat cgcgaagctg cgccggctcg cgcgggtagg cgtgcgccag ttcaacatct
     1501 atctgatgaa ccgcgacgag gaagagcagc tcgagatcta cggcgagaag atcatcccgg
     1561 ccctgaagga cgtcgagacc gtcgcgatcg cgtaaacgtg tggctgcccg ctggcctcgc
     1621 gttcgcgtga ccgggcgata cagcgctcga tgacctcttt cacacgcgcc gccgacgtga
     1681 catcgtgccg cacggcggag cggctgggcg aagcgcaggg gaggtgtgcg tgaacagtcc
     1741 caccagcacc atcgagctcg tgacggacct gagccaggat cgcccgggcg agatcgcaaa
     1801 ggccgtgacg gcgatcgcga acatgagcat caacatcgaa ggcttctgcg aggtcgacgg
     1861 caggctccat ctcgtgacga gcgacccgga gaacgcgcgc aaggcgctcg agaccgtagg
     1921 gttcgtggtg gaggagcgtg agatcttcgt catcgaggcg gacgacaagc cgggcttcct
     1981 cgcgaacatc ctgcgccggc tctcggcgga ggagctcaac atcatcgcga cctacacgct
     2041 cacgaagaca cgcctcgcat tcacggtgga tcagcccgcg cgcatgcggg acatcctgcg
     2101 ggatctctcg ccgacgacga cacgcgttcg ttagcctcgg ccgctccgcc aggcccgcgt
     2161 cacggtcgcg cggcggtcag cgcgagggga tgacgctcct cgcggtcatc gccgttcgcg
     2221 tatcgcgccg agctcccggc cgtacgccca cacgtcgagc tccggcgccg cgagaccgtg
     2281 cgccccgagg atcgtcccga tgtgcgcgcg gtgatcgctc ccgtggtggg tgaactgcgt
     2341 gaccatcatc cacgcctcga gctccagctg cgggtcggtc gggccgcgct gccgggtgtc
     2401 caggaccacg cgatccggat cgaactcccg ttccgcgaag aggttctcga cgccggcctg
     2461 cacgagcgcg agatgcgagg cgatctcgtc gagcggcacg tcgtggtcct cacggaacgg
     2521 cgggtcggcc gtggcgatgc cgaggcgcag caggtagcgc tcgtccgcac cgacgatgtg
     2581 ttcgagcgtg cggcggatcg ttccgtacgt gccgggcgcg gtgagctcga gctgcgcgtc
     2641 ggagagcgtg cggcagtgcg cgatgagccg ctcggtggcc cagat
//
LOCUS       DASTZR010000326         3896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_175135, whole genome shotgun sequence.
ACCESSION   DASTZR010000326 DASTZR010000000
VERSION     DASTZR010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175135"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..693)
                     /locus_tag="VFV20_07280"
     CDS             complement(205..693)
                     /locus_tag="VFV20_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007532502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU5288187.1"
                     /translation="MTTTTKQQAGTSIVTTPTDREIRVERVFNAPREVVWRAMTEPTL
                     VAQWWGRGNKLDVERLEVKPGGHWRYVEHHDGGSDGFEGRFAEVVRPERVSQSFEWDG
                     MPGHPCLNTASLEDLGDGRTRLVQTSLFFTKEERDGMIQSGMEQGMNQSFAELDKLLA
                     TL"
     gene            complement(690..1034)
                     /locus_tag="VFV20_07285"
     CDS             complement(690..1034)
                     /locus_tag="VFV20_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU5288188.1"
                     /translation="MVHYSSRLDTAFGAISDPTRRGILERLGRGDASIGDLASAFDMT
                     LTGMKKHVQVLEDARLVTTKKVGRVRRCSVGPRRLDDETAWIARYRAMVEERMQRLDA
                     VLEREMKKGDRR"
     gene            complement(1113..2453)
                     /locus_tag="VFV20_07290"
     CDS             complement(1113..2453)
                     /locus_tag="VFV20_07290"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HEU5288189.1"
                     /translation="MSDEIRMLPPAYHAAVEHWQRHNFPEYPELAKRWQQYFPKDQEF
                     CLCARIEKDMPAEIQVGDHKGKPNAIQPVELDREAAEQLLMIIRAQASTEFGSIQQHH
                     GTFERANDPQDQTWVLRVMAEELRHGYQMIHLLTSADWSSVTDTKPQDMVEEILSMQT
                     GSHVLAAFNLEYDSFGDSIVFAAFIDRVGKYQLTMQKVNAYAPMAASMPPMLREEAFH
                     LAAGVIPMRRWMEAAARDRNATLDVTTIQKMVNKWFCRGLEMFGDERGGQTNVKLGLK
                     DMANREAQDRYIAECEKMLDDINERYVRARLEGRSRDDALAAFERIKKGETVEGIRRD
                     QLLCLPDRRFFRRRGEPAFQMIGVDGEQFTDVEDYIAHCLAHLPEAYHASIDVKHWIE
                     MQRKVAKGEIELKAAVNSMPRLARVGGACPCSKSVRWVMDVPAGAAGGAEQLNP"
     gene            complement(2514..2951)
                     /locus_tag="VFV20_07295"
     CDS             complement(2514..2951)
                     /locus_tag="VFV20_07295"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5288190.1"
                     /translation="MAEVKIRAVTELDIESICKIDERITGKYRPDVWEDRVIYYIRRD
                     PDSSQVAELDGTVVGFMLGEVRGGEFGLEEPTGWVEFFGVDPKARGTGVGRSLVEALF
                     AGFKRRGAHVARTMVANADKDIDAFIRRMGFAPAPVTALEKRL"
     gene            complement(2967..>3896)
                     /locus_tag="VFV20_07300"
     CDS             complement(2967..>3896)
                     /locus_tag="VFV20_07300"
                     /inference="COORDINATES: protein motif:HMM:NF025016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEU5288191.1"
                     /translation="AGRAPFLGDLLAKLVIAGQNVGGATLTRFYATHVFLIPAAIFGL
                     LAVHLYLVIKRGISEPPEPGERVHTSTYRQRYAEILRRGIPFYPDAAYRDAIVAFVAV
                     VIVVALGLVVGAPALGKQADPTVVVADPKPDWYFLGYFSLLALIPSKAESLVIIGLPT
                     FAILYLFALPIIFPTGERHWSRRPAAIAVVVIPFVAYAALTVAAYTPTWVPVMAADAK
                     VPDAVTRSLAPNEQHGAIVFVQKSCFACHTIAGAGGRRGPDLSDAGARIPRDRIVQRI
                     LTGGGGAMPAYSGSISPQDLDDLVNFLETRQGK"
BASE COUNT          586 a         1335 c         1320 g          655 t
ORIGIN      
        1 cacaggacga ccgggtcgta gtcgaggctc gcgttggtcc agatgctctg gtagccgagt
       61 gcacgcgcgg ccggcgcgag atcgcgcagc tgcgcggtcg tgaggccgag acgttgatcg
      121 atgccgacgc cgatctgcat gtggcggcga gtgtattgac cgtgggcgcg gcccagagca
      181 gggccgcgcc cgccgtcgcg ttcgctacag ggtcgcgagc agcttgtcga gctccgcgaa
      241 gctctggttc atcccctgct ccatgccgga ctggatcatc ccgtcgcgct cttccttcgt
      301 gaagaagagc gacgtctgga ccaagcgcgt gcggccgtcg ccgaggtcct cgagggacgc
      361 ggtgttgagg cacgggtggc cgggcatgcc gtcccactcg aagctctgcg acacacgctc
      421 ggggcggacg acctccgcga accgaccctc gaagccatcg ctcccgccgt cgtggtgctc
      481 gacgtagcgc cagtggccgc cgggcttcac ctcgaggcgc tcgacgtcca gcttgttccc
      541 gcgcccccac cactgcgcga cgagcgttgg ttcggtcatc gcccgccaaa cgacctcgcg
      601 cggcgcgttg aagacccgct cgacgcggat ctcgcggtcc gtgggtgtgg tcacgatgga
      661 tgtgcccgcc tgctgtttcg tcgtcgtggt catcgccggt cccctttctt catctcgcgt
      721 tccagtaccg cgtccaaccg ctgcatgcgt tcttcgacca tggcccgata ccgcgcgatc
      781 caagccgtct cgtcgtcgag gcggcgcgga ccgacgctgc agcgacgcac acgacccacc
      841 ttcttcgtcg tgacgagtcg agcgtcctcg agcacctgca cgtgcttctt catccccgtg
      901 agggtcatgt cgaaggccga cgcgaggtca ccgatcgagg cgtccccgcg cccaagacgc
      961 tccaggatgc cgcgccgcgt cgggtctgag atcgccccga acgccgtatc cagccggctc
     1021 gaatagtgaa ccatacggtt cagtttctac gccactcgga aaggactgtc aagaccaccg
     1081 tttgaagctc ggctggctgc ccgaacgggg tgctaggggt tgagctgctc ggcgccgccg
     1141 gccgcgcccg cgggcacgtc catcacccag cggacggact tcgagcaggg gcacgcgccg
     1201 ccgacacgcg cgaggcgcgg catcgagttg accgcggcct tgagctcgat ctcccccttc
     1261 gccaccttcc gctgcatctc gatccagtgc ttgacgtcga tggacgcgtg gtaggcctcg
     1321 ggcagatgcg cgaggcagtg cgcgatgtag tcctcgacgt cggtgaactg ctcgccgtcc
     1381 acgccgatca tctggaacgc gggctcgccg cggcggcgga agaagcgccg gtcagggagg
     1441 cagagcagct ggtccctccg gatgccctcg accgtctcgc ccttcttgat gcgctcgaac
     1501 gccgcgagtg cgtcatctcg tgagcggccc tcgagccgcg cgcggacgta gcgctcgttg
     1561 atgtcgtcga gcatcttctc gcactcggcg atgtagcggt cctgcgcctc gcggttcgcc
     1621 atgtccttga ggcccagctt cacgttcgtc tgcccgccgc gctcgtcgcc gaacatctcg
     1681 agcccgcggc agaaccactt attgaccatc ttctggatcg tcgtcacgtc gagtgtcgcg
     1741 ttgcggtcgc gcgccgcggc ctccatccag cgccgcatcg ggatcacgcc cgcggcgagg
     1801 tggaacgcct cctcgcgcag catcggcggc atcgacgcgg ccatcggcgc gtacgcgttc
     1861 accttctgca tggtgagctg gtacttcccc acccggtcga tgaacgccgc gaacacgatg
     1921 ctgtcgccga agctgtcgta ctcgaggttg aacgccgcga gcacgtgcga gccggtctgc
     1981 atggagagga tctcctcgac catgtcctgc ggcttggtgt ccgtcacgct cgaccagtcg
     2041 gccgaggtca gcagatggat catctggtag ccgtggcgga gctcctccgc catgacgcgc
     2101 agcacccagg tctggtcctg cggatcgttc gcgcgctcga aggtcccgtg gtgctgctgg
     2161 atcgagccga actcggtcga ggcctgcgcc cggatgatca tgagcagctg ctccgcggcc
     2221 tcccggtcga gctccaccgg ctggatcgca ttcggcttcc ccttgtggtc gccgacctgg
     2281 atctcggcgg gcatgtcctt ctcgatacgg gcgcacaggc agaactcctg gtccttgggg
     2341 aagtactgct gccagcgctt cgcgagctcg gggtactcgg ggaagttgtg gcgctgccag
     2401 tgctccacgg ccgcgtggta ggccggcggg agcatgcgga tctcgtcaga catatgtcta
     2461 ggttaccgtc cggcgttcgc tattggtatg accgttcgcc atggcgaacg ccgctataac
     2521 cgcttctcga gcgcggtgac gggcgccggg gcgaagccca tgcgccggat gaacgcgtcg
     2581 atgtccttgt cggcgttcgc caccatcgtc cgcgccacgt gggcgccgcg ccgcttgaag
     2641 ccggcgaaca gcgcctcgac aagggagcgc ccaacgcccg ttccgcgcgc cttcggatcc
     2701 acgccgaaga actcgaccca gccggtcggc tcctcgagac cgaactcgcc gccgcgcacc
     2761 tcgccgagca tgaagccgac gacggtcccg tcgagctccg ccacctgcga cgagtccggg
     2821 tcgcggcgga tgtagtagat gacgcggtcc tcccagacgt ctgggcggta cttcccggtg
     2881 atgcgttcgt cgatcttgca gatcgactcg atgtcgagct cggtcacggc gcggattttc
     2941 acctcagcca tgccgcgagt atgccgctac tttccctggc gcgtctcgag gaagttgacg
     3001 aggtcgtcga ggtcctgcgg cgagatgctg ccggagtacg cgggcatcgc gccgccaccg
     3061 ccggtcagga tgcgctgcac gatgcggtcg cgggggatgc gcgccccggc gtccgaaagg
     3121 tccgggccgc ggcgcccgcc cgcgccggcg atggtgtggc aggcgaagca gctcttctgc
     3181 acgaagacga tcgcgccgtg ctgctcgttc ggcgcgagac tccgggtcac cgcgtcgggc
     3241 accttcgcgt cggcggccat gaccggcacc caggtcgggg tgtacgcggc gacggtgagc
     3301 gccgcgtagg cgacgaacgg gatcacgacc accgcgatcg ccgccggccg ccgcgaccaa
     3361 tgacgctcgc cggtcgggaa gatgatcggc agcgcgaaca ggtacaggat cgcgaacgtg
     3421 ggcagtccga tgatgaccag gctctcggcc ttcgacggga tgagcgcgag gagcgagaag
     3481 tagccgagga agtaccagtc cggcttcggg tccgccacca cgaccgtcgg atcggcctgc
     3541 ttcccgagtg ccggcgcgcc gacgacgagc ccaagggcga ccacgatcac cacggcgacg
     3601 aaggcgacga tcgcgtcgcg gtacgcggca tcgggataga aggggatgcc gcggcgcagg
     3661 atctccgcgt accgctgacg gtaggtcgac gtgtgcacgc gctcgccggg ctcgggcggc
     3721 tccgagatgc cacgcttgat gaccaggtac agatgcacgg cgaggagccc gaagatcgcc
     3781 gcggggatga ggaacacatg cgtggcgtag aaccgcgtga gggtcgcgcc gccgacgttc
     3841 tgtcccgcga tcacgagctt cgccagcagg tcgccgagga acggcgcgcg gccggc
//
LOCUS       DASTZR010000327         6137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_123207, whole genome shotgun sequence.
ACCESSION   DASTZR010000327 DASTZR010000000
VERSION     DASTZR010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6137
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123207"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VFV20_07305"
     CDS             complement(<1..389)
                     /locus_tag="VFV20_07305"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HEU5288192.1"
                     /translation="MEGTGELAWFRGEIWRSLVRPRDFAKDLTREHYGIAGVLVALFA
                     GVGLSIGVDLLVLASKDLPAFRFVGRVLTDSVLLGVRLAVTTAVVAWIAYGGIRVMGR
                     RGGSLDQLFTALTFATAPLILAPLPAVV"
     gene            complement(774..1886)
                     /locus_tag="VFV20_07310"
     CDS             complement(774..1886)
                     /locus_tag="VFV20_07310"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU5288193.1"
                     /translation="MVRLPALDATLGTVERIASAPTAPYHEHRATAAIERELAALGVP
                     TSRDDYAQLYAHVRRGVAKRSLALVAHTDHPAFEITRTAGREGRARILGGFYPRFFAG
                     TVPVLVCDDGDTEPFSAALDDYVAEPDVTHNSPGHCRIRADRPLPANGWAVLDLPAFA
                     VHGDEIHLRAADDLALCAVVVLTLAALREEQRAHDVRAVFTRGEETGLYGARLTAEDG
                     LVPRDAIAVSLEASRALPNARPGAGVVVRPGDMHNTFSNDGERFLRVGRERLAEAKIP
                     TQRSLLTGGTCEASSFVRLGWIATAIAVPNVNYHNQTDEHDQFVPEIVRLSDLRSAVA
                     LLVEAAAAAGADEDESWWPDVRAVPPGIRSLMRRRS"
     gene            1913..3073
                     /gene="cysS"
                     /locus_tag="VFV20_07315"
     CDS             1913..3073
                     /gene="cysS"
                     /locus_tag="VFV20_07315"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU5288194.1"
                     /translation="MLALYDTRRRRTAAFHPRSTVATVYVCGVTPYDTTHLGHARTFL
                     VFDVLVRLLEARGTRVRYAQNVTDIDESILQRAARDGVDWRALGRREERKFLEDMRAL
                     RWRRPDVMPHATRELPAMRALIARLERRDAAYPVDGSVYFDVSRDPRYGELSRLTPAR
                     MRRVMARQDDASLDDPHRRDPLDFALWRKVPDGPAWPSPYGRGRPGWHLECSAMALRH
                     LGERIDIHGGGSDLVYPHHENEIAQSEAATGKRPFSAWWMHPAPVRLDGEKMSKSLGN
                     MVFVRDALRTTSAEALRLYLLDGHYRRPFDHDETRLARARERADRLAAGLGRGPAGPL
                     GRDRATRAVLAALEDDLDTRRAIQTLERAAGTADEATKGSLRSVARTVLGVA"
     gene            3109..4080
                     /locus_tag="VFV20_07320"
     CDS             3109..4080
                     /locus_tag="VFV20_07320"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HEU5288195.1"
                     /translation="MSIGPIAIPDRGGRAAGSFTFDGDEGLARYKWPYIAIRGAHDGP
                     SIVITAGIHAAEYTGIEAAMRLGRSIDPREVRGTIVLIPLLNRPGFYERSIYVNPEDN
                     DNLNRLFPGDANGTWGQRFAHRLLTEVLARFDHAIDLHAGDLIEDLTPFVIYRETGNA
                     ALDAKIVRMVNAYGAEWAVKSAPTGERPGSLYAVAALSGVAAMLAESGGRGLLIEEDV
                     MRHVRGVTNILRAIGILEGEPDRVAPPTVVDSFDWVRSPAEGMFHCGVHVGQRVRVGD
                     LIGDLTDLVGEPIAPVRAPVSGVVLFVVTSPAIKRDGLLLAIGALRG"
     gene            complement(4034..5257)
                     /locus_tag="VFV20_07325"
     CDS             complement(4034..5257)
                     /locus_tag="VFV20_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5288196.1"
                     /translation="MGRGQAAFAFIFVTVLLDMLAFGIIIPVLPQLIVHFEGGDMASA
                     ALIFGVFGTAFALMQFLAAPVLGALSDHFGRRPVVLLSNLGLGLDYVVMAIAPTIPVL
                     FIGRLISGVTASSYATASAYVSDVTPPERRAARFGMLGVAFGIGFIVGPALGGIVAGI
                     DLRAPFWLAAALSFTNFLYGVFILPESLPAERRARFRLRAANPIGAMRFLGSRPTLVT
                     LSLVALFAYLAHDSLPHTFVLYSAYRFAFDERAVGFGLTLAGVSSLVVQGVVVGRAVG
                     ALGERGALLLGLLIGLVAQLILGLAPTATLFLAAIPVWSLFGLVTPSLQSIATREVRP
                     TEYGQLQGALGSLRSIATLFTPLLYTQVFAAAIEPLAPFGLPGAPFVLSALFLAAAAT
                     ISLAGRRSRAAGRPS"
     gene            5226..5852
                     /locus_tag="VFV20_07330"
     CDS             5226..5852
                     /locus_tag="VFV20_07330"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU5288197.1"
                     /translation="MNANAACPRPIAGIIAYLRGLYLTAERLADAARRWGEPRSLSLE
                     FAIGPRERDLVRGSRKRDRSHDITLFIERDGLWAVITKPQFPPGVWRAPSGGLNVGEE
                     LEAGALREALEETGLTVALERYLLHIDARFTDGDAVEPWHSEVFLAPAVAGELAPIDT
                     HEIAGARWMAREELLGPVRERLIASGRGLFGYRAALHDAAFEALDRIR"
     gene            complement(5919..>6137)
                     /locus_tag="VFV20_07335"
     CDS             complement(5919..>6137)
                     /locus_tag="VFV20_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288198.1"
                     /translation="VNASAPIARWSRTAGTSVGGAVGAGVGGAAVGLAAGDGLPSGDD
                     AAVVGEGAFAPNGNEHATVRATVAESAT"
BASE COUNT          937 a         2192 c         2182 g          826 t
ORIGIN      
        1 acgaccgcgg gcaggggagc gaggatcaac ggcgcggtcg cgaacgtgag tgcggtgaac
       61 agctggtcga gcgagccgcc acgccggccc atcacccgga tgccgccgta cgcgatccat
      121 gcgacgacgg cggtggtgac cgccaggcgg acgccgagga gcaccgagtc cgtcagcacc
      181 cggccgacga agcggaacgc cggaaggtcc ttcgatgcga gcacgagcag gtcgacgccg
      241 atggacagtc cgacgccggc gaagagcgcg acgagcacgc cggcgatgcc gtagtgctcg
      301 cgcgtcagat ccttcgcgaa gtcgcgcggg cgcacgagcg aacgccagat ctcgccgcgg
      361 aaccaggcga gctcgcccgt gccttccatg tcctcgacag tacaaaaagc gacccccgcg
      421 tccgggggtc gctttgtgtt cgcttgttaa cagtccgatg gagcgtcggc ttcaggcctc
      481 acttccggcc tcggcgtggc gatcaccact tggggtgatc gtcggtccac tcatcggatc
      541 cgccgcccgc tcctcaggtc gagccccctt ccgccgaggg cgtcatcgat cgacctggaa
      601 gactccgaac actttgtgaa gccggaccat gacacctcct gttgggttgg tgcgttggtc
      661 tttcgttgct ccgtgttccg cggaacaacc ctgttctaca cctgagagag atgccgtcaa
      721 gaggcgggcg cctgacaggt ggtatgagaa aaaagtggta gcgaccggtc gagctaggac
      781 cgccgtcgca tgagcgagcg gatccccggg ggaacggccc gcacgtccgg ccaccagctc
      841 tcgtcctcgt ccgcgcccgc cgccgcggcc gcctcgacga gcagcgccac ggcgcttcgc
      901 agatccgaca agcggacgat ctccggcacg aactggtcat gctcgtcggt ctggttgtgg
      961 tagttgacgt tcggcacggc gatcgccgtc gcgatccatc cgaggcgcac gaacgacgac
     1021 gcttcgcacg taccgcccgt caggagcgag cgctgcgtgg ggatcttcgc ctccgcgagc
     1081 cgctctcgtc cgacgcggag gaagcgctcg ccgtcgttgc tgaacgtgtt gtgcatgtcg
     1141 ccgggccgca cgaccacgcc ggcgccggga cgagcattcg gcagggcgcg gctcgcctcg
     1201 agggagacgg cgatcgcgtc gcgcggcacc agtccgtcct cggcggtcag gcgcgcgccg
     1261 tagagccccg tctcctcgcc gcgcgtgaac accgcgcgca cgtcgtgcgc gcgctgctcc
     1321 tcccgcagcg ccgcgagcgt gagcacgacg accgcgcaca gcgccaggtc gtcggcggcg
     1381 cgcagatgga tctcgtcgcc gtggacggcg aacgccggaa ggtccagtac cgcccacccg
     1441 ttcgccggga gcgggcgatc cgcgcgtatg cggcagtgcc cgggcgagtt gtgtgtcacg
     1501 tccggttcgg cgacgtagtc gtcgagcgcc gcgctgaacg gttcggtgtc gccgtcgtcg
     1561 cacacgagga ccggcacggt gccggcgaag aagcgcgggt agaacccgcc gaggatgcgc
     1621 gctcggccct cgcggcccgc cgtgcgcgtg atctcgaatg ccgggtgatc ggtgtgcgcg
     1681 acgagcgcga gcgatcgctt cgccacaccg cgccgcacgt gtgcgtacag ctgcgcgtaa
     1741 tcgtcgcggc tggtcgggac gccgagcgcc gcgagctcgc gttcgatggc cgcagtcgcg
     1801 cggtgctcgt ggtacggcgc cgtcggcgct gaggcgatcc gctccacggt cccgagcgtg
     1861 gcgtcgagag cgggcaagcg gaccatcgtt cgcgaggata tcgtcaggcg aggtgctcgc
     1921 cctctacgac acacgtcgcc gccgcaccgc cgcgttccac ccgcgctcca ccgtcgcgac
     1981 ggtctatgtc tgcggcgtca cgccgtacga caccacgcac ctcggccacg cgcgaacgtt
     2041 cctcgtgttc gacgtcctcg tgcggctcct cgaggcccgc gggacgcgcg tgcgctacgc
     2101 gcagaacgtc accgacatcg acgagtcgat cctgcagcgc gccgcccgcg acggcgtcga
     2161 ttggcgtgcg ctcggccgac gcgaggagcg gaagttcctc gaggacatgc gcgcgctccg
     2221 ctggcgcagg cccgacgtga tgccgcacgc gacgcgcgag ctccccgcga tgcgggcgct
     2281 catcgcgcgt ctcgagcgcc gggacgccgc gtacccggtg gacggcagcg tgtacttcga
     2341 cgtgtcgcgc gacccgcggt acggcgagct ctcacgcctc acgccggcgc ggatgcgccg
     2401 ggtcatggct cggcaggacg acgcgtcgct cgacgatccg catcgccgcg acccgctcga
     2461 cttcgccctc tggcggaagg tcccggacgg ccccgcgtgg ccgagcccgt acggacgcgg
     2521 tcgtcccggc tggcacctcg agtgctcggc gatggcgctt cgccatctgg gagagcgcat
     2581 cgacatccac ggcggcggca gcgatctcgt ctacccgcac cacgagaacg agatcgccca
     2641 gtcagaggcg gcgaccggga agcggccctt cagcgcctgg tggatgcatc ccgcgcccgt
     2701 gcgccttgac ggcgagaaga tgtcgaagtc gctcgggaac atggtcttcg tgcgcgacgc
     2761 gctgaggacg acgagcgccg aggcgctgcg cctctatctg ctcgacggcc actaccgcag
     2821 gccgttcgat cacgacgaga cgcggctcgc gcgagcgcgc gagcgcgccg accggctcgc
     2881 ggctgggctc ggacgcggac ccgccggtcc gctcggccgc gaccgcgcga cgcgcgcggt
     2941 gctcgccgcc ctcgaggacg atctcgacac gcgccgagcg atccagacgc tcgagcgcgc
     3001 ggccggcacc gcggatgagg cgacgaaggg atctctgcgc tccgtcgcgc ggacggtgct
     3061 cggcgtcgcc tgacgccacg tcccggcgct cgcgtaccgt agtggccgat gtccatcggc
     3121 ccgatcgcca tccctgaccg cggcgggcgc gccgcgggct cgttcacctt cgacggtgat
     3181 gagggcctcg ccaggtacaa gtggccatac atcgcgatcc gcggcgcgca cgacgggccg
     3241 tccatcgtca tcaccgcggg catccacgcg gcggagtaca cgggcatcga ggcggcgatg
     3301 cgcctcggcc gctcgatcga tccgcgcgag gtgcggggca cgatcgtgct catcccgctc
     3361 ctcaaccgcc ccggctttta cgagcgcagc atctacgtga atcccgagga caacgacaac
     3421 ctgaaccggc tgttccccgg cgacgcaaac ggcacgtggg gccagcgctt cgcgcaccgc
     3481 ctgctcaccg aggtgctcgc gcgcttcgat cacgcgatcg acctgcacgc cggcgacctc
     3541 atcgaggacc tcacgccgtt cgtgatctac cgcgagaccg ggaacgccgc gctcgacgcg
     3601 aagatcgttc gcatggtgaa cgcgtacggc gcggagtggg cggtgaagag cgcgccgacc
     3661 ggcgagcgtc ccggcagcct gtacgcggtc gcggcgctct cgggcgtcgc cgcgatgctc
     3721 gcggagtcgg gcggccgcgg gctgctcatc gaagaggacg tcatgcgcca tgtccgcggg
     3781 gtcacgaaca tcctccgcgc catcggcatc ctcgagggcg agcccgaccg cgtggcgccg
     3841 cccaccgtcg tcgacagctt cgattgggtg cgctctcccg cggaaggcat gttccactgc
     3901 ggcgtacacg tcggtcagcg tgtgcgggtg ggggacctca tcggcgacct caccgacctc
     3961 gtcggcgagc ccatcgcgcc ggtgcgcgcg ccggtgagcg gcgtcgtgct cttcgtcgtc
     4021 accagccccg cgatcaagag ggacggcctg ctgctcgcga tcggcgccct gcgaggctga
     4081 tcgtcgcggc ggccgccagg aagagcgcgc tcaggacgaa gggcgcaccg ggcagcccga
     4141 acggggcgag cggttcgatc gcggcagcga agacctgcgt gtagaggagc ggcgtgaaga
     4201 gcgtcgcgat ggagcgcagg ctcccgagcg cgccctggag ctgaccgtac tcggtgggcc
     4261 ggacctcgcg cgtcgcgatg gactgcagcg acggtgtgac gagcccgaag agcgaccaga
     4321 ccgggatcgc cgcgaggaac agtgtcgcgg tgggcgcgag cccgaggatg agttgggcga
     4381 cgaggccgat gagcaacccg agaagcaggg cgccgcgctc gccgagcgcg ccgaccgcgc
     4441 ggcccacgac caccccctgc acgacgagcg acgagacgcc cgcgagcgtg agcccgaacc
     4501 cgaccgcacg ctcgtcgaac gcgaagcgat acgcgctgta gaggacgaac gtatgcggca
     4561 gcgagtcgtg cgccaggtac gcgaagagcg cgacgagcga cagtgtgacg agcgtcgggc
     4621 ggctgcccag gaatcgcatc gcgccgatgg gattcgcggc ccgcaggcgg aagcgggcgc
     4681 gccgctcggc cggcagcgat tcgggaagga tgaacacgcc gtacaggaag ttcgtgaacg
     4741 acagcgccgc ggcgagccag aagggcgcgc gcaggtcgat gccggccacg atgcccccga
     4801 gggccggtcc gacgatgaag ccgatgccga acgcgacgcc gagcatcccg aaacgcgccg
     4861 cacgccgctc cggcggcgtg acatccgaca cgtatgcgct cgcggtcgcg tacgacgacg
     4921 ccgtgactcc cgatatgagg cgtccgatga agaggaccgg gatcgtcggc gcgatggcca
     4981 tgacgacgta gtccaggccg agccccaggt tcgagagcag caccaccggg cgccgcccga
     5041 agtgatcgga cagcgcgccc agcaccggcg ccgccaggaa ctgcatgagc gcgaacgcgg
     5101 tgccgaagac gccgaagatg agcgccgcgc tcgccatgtc gccgccctcg aagtgcacga
     5161 tgagctgggg gaggaccggg atgatgattc cgaacgcgag catgtccaag aggaccgtca
     5221 cgaagatgaa cgcgaatgcc gcttgtcccc gccccatcgc cggaatcatc gcgtatcttc
     5281 gaggtctgta cctcaccgcg gaacgtctcg ccgatgccgc gcgccggtgg ggcgagccgc
     5341 gctcgctctc gctcgagttc gctatcggcc cgcgcgagcg tgaccttgtg cgtgggagcc
     5401 gcaagcgtga ccggtcccac gacatcacgc tgttcatcga gcgcgacggc ctgtgggccg
     5461 tcatcacgaa gccgcaattc ccgccgggcg tgtggcgcgc gccgagcggc gggctgaacg
     5521 tcggcgagga gctcgaggcc ggcgcgctgc gcgaggcgct cgaggagacg ggcctgacgg
     5581 tggcgctcga gcgctacctg ctgcacatcg acgcgcgctt cacggacggc gacgcggtcg
     5641 agccgtggca cagcgaggtg ttcctcgcgc ccgcggtcgc gggcgagctc gcgccgatcg
     5701 acacccacga gatcgccggc gcgcgttgga tggcgcgcga ggagctgctc ggtccggtgc
     5761 gcgagcggct catcgcatcg gggcgcggtc tcttcgggta ccgcgccgcg ctgcacgacg
     5821 ccgccttcga ggcgctcgac cgcatccgct gacgccggga cggccgttcg cacaccgccc
     5881 gcgcccacgc gctgatagcg tgacgacatg gcattccgtc atgtcgcgct ctccgcgacc
     5941 gtcgctctca cggtcgcgtg ctcgttgccg ttcggcgcga acgcgccctc gccgaccact
     6001 gccgcgtcat caccgctcgg gagcccgtcg cccgccgcca gcccgacggc ggcacctccg
     6061 acgccggccc cgaccgcgcc gcccaccgac gtgcccgcgg tgcgactcca gcgcgcgatg
     6121 ggcgcgctcg cgttcac
//
LOCUS       DASTZR010000328         2939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_18816, whole genome shotgun sequence.
ACCESSION   DASTZR010000328 DASTZR010000000
VERSION     DASTZR010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18816"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..1134
                     /locus_tag="VFV20_07340"
     CDS             73..1134
                     /locus_tag="VFV20_07340"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU5288199.1"
                     /translation="MEELLASETEGSLRPLRTGETVEGTVAAISGDEATIDLGDRPAG
                     VIPVREAGTEQLHVGESLTATVVQPEGPDGRVVLSLRRARHRRQWTQMEELQRSGELI
                     EAAVIDANRGGVVVDVGMRGFVPLSQLGSIGAIDTTEAGVPEPVRGLVGKRLRLRVLE
                     VDPKRDRLILSEKAATQQLRRERKAQGAARLAEGDVLEGTVVSVTSYGVFVDVGVADG
                     LVHRSELTWEKGVEPTSLFTVGQSVRVMVVGIDRERQRISLSIKRLADDPWDRFTKSV
                     QVGQSVDATVTRVMPYGAFAKVADGVEGLVHVSELAPQRVADPNEVVHAGDTLRVKIV
                     GVDAERRRLSLSARLAERT"
     gene            1223..>2939
                     /locus_tag="VFV20_07345"
     CDS             1223..>2939
                     /locus_tag="VFV20_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU5288200.1"
                     /translation="MMGPFDRFNDRAKRVLALAQDEAIRFNHNYIGTEHLLLGLVREG
                     EGVAARVLDSLGVELSKVRTAVEFIIGRGDSTTSPSEITLSPRTKKVIELAIDEARKL
                     GHSHVGTEHLLLGLVREGEGIASGVLESLGVSLEKVRHQVIATLGQQHPATAEAASTG
                     AGGKASQSKTPTLDQLGVNLTAMAKAQQLDPVIGREKEIERVIQILSRRTKNNPALIG
                     EPGVGKTAIAEGLAHRIVKGDVPETLQGKRVLTLDIGSLVAGTKYRGEFEERLKKIIE
                     ELRHAHDVILFIDELHTLVGAGAAEGAVDAANILKPSLARGELQCIGATTLDEFRKYI
                     ERDAALERRFQPVMVEEPSIEETQAILMGIRSRYEEHHKVSITDDAVKAAAELSARYI
                     TDRFLPDKAIDLMDEAASRVRLRYSTLPTDVKDAQKELDKVVTEKESAMEAQQYELAS
                     TLRQKERAQQDKLNQAKADWILAQSQKRPEVTDEDIAEVVSMWTGIPVRRLAEEETQK
                     LLKMEETLHAKVIGQEAAVTTISRAVRRARAGLKDPRRPIGVFFFVGPTGTGKTHLAR
                     QLAEFM"
BASE COUNT          505 a         1000 c         1029 g          405 t
ORIGIN      
        1 cggtttgagg gattgtatcg gtggcaccgg atatgcccgc ctgctcgggg tatgtccgtc
       61 gaccctgaga cgatggaaga gctcctcgca tccgagaccg agggatcgct ccgaccgctg
      121 cgcaccgggg agacagtgga aggcaccgtc gccgcgatca gcggcgacga agcgacgatc
      181 gatctcggcg accgccccgc gggcgtcatc ccggtgcgcg aggccggtac cgagcagctg
      241 cacgtgggcg aatcgctcac cgcgaccgtc gtccaacccg agggtccgga cgggcgcgtc
      301 gtcctttcat tgcgacgcgc tcgacaccgc cgtcagtgga cgcagatgga ggagcttcag
      361 cgctcgggcg agctcatcga agccgcggtc atcgacgcga accgtggcgg cgtggtcgtc
      421 gacgtcggca tgcgcggctt cgtcccgctc tcgcagctcg gatcgatcgg ggcgatcgac
      481 acgaccgagg cgggcgtccc cgagccggtg cgcgggctcg ttgggaagcg gctgcgcctg
      541 cgcgtgctcg aggtggatcc caaacgcgac cggctcatcc tctctgagaa ggccgccacg
      601 cagcagctgc ggcgggagcg caaggcgcag ggggcggcgc ggctcgccga gggggatgtc
      661 ctcgagggga cggtcgtgag cgtcacctcc tacggcgtct tcgtcgacgt cggcgtggcc
      721 gacgggctgg tccatcgctc ggagctcacg tgggagaagg gcgtcgagcc gacatcgctg
      781 ttcacggtgg ggcagagcgt ccgggtgatg gtcgtcggca tcgaccggga gcgccagcgc
      841 atcagcctct cgatcaagcg gctcgcggac gacccgtggg accggttcac aaagtcggtg
      901 caggtgggcc agtcggtgga cgccacggtc acgcgcgtca tgccgtacgg cgcattcgcc
      961 aaggtggcgg acggcgtgga gggcctggtc cacgtcagcg agcttgcgcc ccaacgcgtg
     1021 gcggatccga acgaggtcgt gcacgccggc gacacgcttc gcgtgaagat cgtgggcgtg
     1081 gatgccgaac ggcggcggct gtcgctgtcc gcccggctcg ccgagcgcac ctgagcgctt
     1141 gtctggagcg gcttcatatg tctttccgaa cgtataatcg gaaccagatg agacctcgac
     1201 cgatatacag gtacaaggca cgctgatggg acccttcgac cgcttcaacg accgcgcgaa
     1261 gagggtcttg gcgcttgccc aggacgaggc gatccgcttc aaccacaact acatcggcac
     1321 cgagcacctt ctcctgggcc tcgtccgcga gggcgagggc gtggccgcga gggtcctgga
     1381 ctccctcggc gtcgagctct ccaaggtgcg gacggcggtc gagttcatca tcggacgcgg
     1441 cgactcgacc acctcgccga gcgagatcac gctctcgccg cgcaccaaga aggtcatcga
     1501 gctcgcgatc gacgaggccc gcaagctcgg ccacagccac gtcgggaccg agcacctcct
     1561 gctcggcctc gtgcgcgagg gcgagggcat cgcgtcgggc gtcctcgagt cgctgggcgt
     1621 gtcgctcgag aaggtccgtc accaggtcat cgcgacgctc ggccagcagc acccggccac
     1681 cgcggaggcg gcgtccacgg gtgcgggcgg caaggcgtcg cagtcgaaga cgcccacgct
     1741 cgaccagctc ggcgtcaacc tcacggcgat ggcgaaggcc cagcagctcg acccggtcat
     1801 cggccgcgag aaggagatcg agcgcgtcat ccagatcctc agccggcgca cgaagaacaa
     1861 cccggcgctc atcggcgagc cgggcgtcgg caagaccgcc atcgcggaag gcctcgcgca
     1921 ccgcatcgtg aagggcgacg tgccggagac gctgcagggc aagcgcgtgc tcaccctcga
     1981 catcggctcg ctcgtcgcgg gcacgaagta ccgcggcgag ttcgaggagc gcctcaagaa
     2041 gatcatcgag gagctccgcc acgcgcatga cgtcatcctc ttcatcgacg agctgcacac
     2101 gctcgtcggc gccggtgcgg cggagggcgc ggtcgacgcc gcgaacatcc tcaagccgtc
     2161 gctcgcgcgc ggcgagctgc aatgcatcgg cgcgacgacg ctcgacgagt tccggaagta
     2221 catcgagcgc gacgccgcgc tcgagcgccg cttccagccc gtgatggtcg aggagccctc
     2281 catcgaggag acgcaggcca tcctcatggg catccggtcc cggtacgagg agcaccacaa
     2341 ggtctccatc accgacgacg cggtcaaggc ggccgccgag ctctcggcgc gctacatcac
     2401 cgaccgcttc ctgccggaca aggccatcga cctcatggac gaggccgcga gccgcgtccg
     2461 cctgcgctac tccacgctgc cgacggacgt gaaggacgcg cagaaggagc tcgacaaggt
     2521 cgtcaccgag aaggaatcgg ccatggaggc gcagcagtac gagctcgcct cgacgctccg
     2581 ccagaaggag cgcgcccagc aggacaagct caaccaggcg aaggcggact ggatcctcgc
     2641 gcagtcacag aagcgtcccg aggtgaccga cgaggacatc gccgaggtcg tgtcgatgtg
     2701 gactggcatc cccgtgcgcc gcctcgccga ggaggagacc cagaagctcc tgaagatgga
     2761 ggagacgctc cacgcgaagg tcatcggcca ggaggccgcc gtcacgacga tctcccgggc
     2821 cgtccgccgc gcgcgcgccg gcctgaagga cccgcggcgt ccgatcggcg tgttcttctt
     2881 cgtcgggccc acgggcaccg gcaagacgca cctcgcgcgt cagctcgccg agttcatgt
//
LOCUS       DASTZR010000329         2778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_619426, whole genome shotgun sequence.
ACCESSION   DASTZR010000329 DASTZR010000000
VERSION     DASTZR010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619426"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="VFV20_07350"
     CDS             complement(<1..1088)
                     /locus_tag="VFV20_07350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5288201.1"
                     /translation="MKVALAHEYFSVHGGAEAVVEVFHAMWPDAPVYTFFHDRKRYGE
                     LPGWRVVTSTLQGFPIGGGLHRLLLPLYPTAARHLRVPSDVDVALVSTSAFIKGLALA
                     EATVEVAYCHSPTRYLWDWTEQYLDEEVPPALHRPVRELLAHLRDVDRDMAQRVDQWV
                     ANSAVVAERIRRFYGRESEVLHPPVAVDGFEARPERGDHWLFVGRLSAYKRADVAVRA
                     FARSRRRLIVVGEGRERDALERLATDNIVFTGRVDDATKRDLLASARGLVFPAEDDFG
                     IVCAEALASGAPVVALARGGAPEIVRDGLDGALFAEADAEQVAAAVDRVDAQRWDPQA
                     LRTSARRFDVGRFRDRIGAIVESSARSGR"
     gene            complement(1085..1813)
                     /locus_tag="VFV20_07355"
     CDS             complement(1085..1813)
                     /locus_tag="VFV20_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEU5288202.1"
                     /translation="MSGRVSVLGVDFDRLDLPEAWREILARLDRGERTFVITANPEFV
                     MLARGDRAVADVARTADVVVADGAGIVAASRILGDPLARVPGRLLVDALVPAFAERGA
                     SLFLLGAAPGIAERAAEVLCARTPRLRIAGCYAGAAADDAETVARVRDAAPDVLLVAY
                     GMPRQEAWIARNLPSLPSVKIAIGVGGVFDQLAGLRPVPPDVLHRFGLEWLWRLAWEP
                     RRWRRQRVLPLFALLVLRARLLGR"
     gene            complement(1839..>2778)
                     /locus_tag="VFV20_07360"
     CDS             complement(1839..>2778)
                     /locus_tag="VFV20_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEU5288203.1"
                     /translation="LTEPSVVALAHDEGTNRPRIVAVGEEARLMLGRTPGNITALRPM
                     RDGVIADYVITQHMLKHFVDKVCGRVRIFHPQLMICIPSGGTSVEQRAVKDAAIRAGA
                     REAFLIPEPLAAAIGANIPISGPSGNLIIDIGGGTAEIAVISLGSIVVSRSVRAAGNK
                     IDEAIAAFVRKRYNLMIGERTAEDVKIQIGSALPMDPELTMEVRGRDLIAGLPRTITI
                     GSNEITEAMDPVLQQIVAAVKSVLEETPPELASDVIDKGMVLSGGGALLRNLDKLLTQ
                     VTGVACYVAENPLYCVAKGTGLALEHLPFFRRSLVK"
BASE COUNT          431 a          985 c         1004 g          358 t
ORIGIN      
        1 cgtccgctgc gcgcggacga ctcgacgatc gcaccgatgc gatcgcgaaa ccggcccacg
       61 tcgaaacgtc tcgcggaggt gcgcagcgcc tgcgggtccc atcgctgtgc gtcgacccgg
      121 tcgacggccg ccgcgacctg ctccgcatcc gcttcggcga acagcgcgcc gtcgagaccg
      181 tcgcgaacga tctcgggcgc gccgcccctc gcgagcgcga cgaccggcgc acccgacgcg
      241 agcgcctcgg cgcacacgat cccgaagtcg tcctcggctg ggaacaccag accgcgggcc
      301 gaggcgagca ggtcgcgctt ggtcgcgtca tcgacgcggc cggtgaagac gatgttgtcc
      361 gtcgcgagcc gctcgagcgc gtcacgctcg cgaccctcgc cgacgacgat gagacggcga
      421 cgcgagcgcg cgaacgcgcg cacggcgacg tcagcgcgct tgtacgcgga cagccggccc
      481 acgaagagcc agtggtcgcc gcgctccggg cgcgcctcga acccatccac ggcgaccggg
      541 gggtgaagga cctcgctttc gcgtccgtag aagcggcgga tgcgctcggc gacgaccgcg
      601 gagttcgcga cccactggtc gacgcgctgc gccatgtcgc gatcgacgtc gcgcagatgc
      661 gcgaggagct cgcggaccgg tcgatggagg gcgggcggga cctcctcgtc gaggtactgc
      721 tccgtccaat cccagagata tcgcgtcggc gagtggcagt aggcgacctc gaccgttgcc
      781 tccgccagcg cgaggccctt gatgaacgcg ctggtggaga cgagcgcgac gtcgacatcg
      841 ctcggcacgc ggaggtgacg cgccgcggtg ggatacagcg gcagcagcag tcgatgcagg
      901 cccccgccga tcgggaagcc ctgcagggtg gaggtgacga cccgccatcc cggcagctcg
      961 ccgtaccgct tccggtcgtg gaagaacgtg tacaccggtg cgtcgggcca catcgcgtgg
     1021 aagacctcga cgaccgcctc cgccccaccg tggacggaga agtactcgtg cgcgagcgcg
     1081 accttcatcg gccgagcagc cgcgcgcgca ggacgagcag ggcgaagagc ggcagcacgc
     1141 gctgacggcg ccagcgccgc ggctcccagg cgaggcgcca cagccactcg aggccgaagc
     1201 gatgcagcac gtcgggcggg acgggacgga gcccggcgag ctggtcgaag acgccgccga
     1261 caccgatggc gatcttcacc gaaggcagcg acgggaggtt gcgcgcgatc cacgcctcct
     1321 gcctcggcat cccgtacgcg acgaggagga cgtcgggggc ggcgtcgcgg acgcgcgcca
     1381 cggtctcggc atcgtccgcg gccgctccgg cgtagcagcc cgcgatccgc agccgcggcg
     1441 tgcgcgcgca gaggacctcg gccgcgcgct cggcgatgcc cggcgcggca ccgaggagga
     1501 agagcgaggc gccgcgctcc gcgaacgccg ggacgagcgc atccacgagc agccgtccgg
     1561 gcacgcgcgc gagcgggtcg ccgaggatcc gcgacgcggc gacgatgccg gcgccatccg
     1621 cgacgacgac atcggccgtg cgggcgacgt ccgccacggc gcggtcgccc cgcgcgagca
     1681 tcacgaactc gggatttgcg gtgatgacga aggtgcgctc gccgcggtcc agccgcgcga
     1741 ggatctcgcg ccaggcctcg ggcaggtcga gacgatcgaa atccactccg aggaccgaga
     1801 cgcgcccgct cacgcgggct aacgctagcg ccacgtcgtc acttgacgag gctgcgacgg
     1861 aagaacggaa ggtgctcgag cgccagcccg gtccccttcg cgacgcagta cagcgggttc
     1921 tccgccacgt agcacgcgac gccggtgacc tgcgtgagga gcttgtccag gttgcgcagg
     1981 agcgcgccgc cgcccgagag caccatgccc ttgtcgatca cgtccgacgc gagctcgggc
     2041 ggggtctcct cgagaacgct cttgaccgcg gccacgatct gctgcagcac cggatccatc
     2101 gcctcggtga tctcgttgct gccgatcgtg atcgtccgcg ggaggccggc gatgaggtcg
     2161 cggccgcgca cctccatcgt cagctcggga tccatcggca gcgcgctgcc gatctggatc
     2221 ttcacgtcct ccgcggtgcg ctcgccgatc atcaggttgt agcgcttgcg cacgaacgcg
     2281 gcgatcgcct cgtcgatctt gttgcccgcg gcgcgaacgc tccgcgacac gacgatgctg
     2341 ccgagcgaga tgaccgcgat ctcggcggtc ccgccgccga tgtcgatgat gaggttgccg
     2401 ctcggacccg atatcgggat gttcgcgccg atcgcggcgg cgagcggctc ggggatgagg
     2461 aacgcctcgc gcgctccggc gcggatcgcg gcgtccttca ccgcgcgctg ctcgacgctc
     2521 gtgcctccgg acgggatgca gatcatgagc tgcggatgga agatgcgcac ccggccgcag
     2581 accttgtcga cgaagtgctt gagcatgtgc tgcgtgatga cgtagtcggc gatcacgccg
     2641 tcacgcatcg ggcgcagcgc ggtgatgttg cccggcgttc gaccgagcat gaggcgcgcc
     2701 tcctcaccga cggcgacgat gcgcgggcgg ttcgtcccct catcgtgcgc gagcgcgacg
     2761 accgacggct cggtgagg
//
LOCUS       DASTZR010000330         4369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_723148, whole genome shotgun sequence.
ACCESSION   DASTZR010000330 DASTZR010000000
VERSION     DASTZR010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723148"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1389
                     /locus_tag="VFV20_07365"
     CDS             <1..1389
                     /locus_tag="VFV20_07365"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEU5288204.1"
                     /translation="YAGIAAAFIAPRLSDMLAFFLGISTIFSDAGAVTVTVAVLSLVA
                     VLSASEAALHSVRRPEMIEELALRGRRGRRAGTMGRRRAHYLAAIQVSEFLIVFAYAG
                     IAAAFIAPRLSALLKFLLGIDAIFTDVSAVVVTVSVLSLVAVLFGLFVPRAIAARHAQ
                     GVLLGLVWPLEVVTWGTRPLVSMLFLLTQAIARPFGANPQAGTLVTEEEIRALVETGQ
                     AQGVLEPKEREMISSIFELGEKEVREVMIPRTDLVALDVTTPIRQVLDEITRIGHSRL
                     PVYEGSADEIVGILYVKDLFRKLARGERELELRRLLRPVHFVPETKKVDDLLREMQRD
                     KMHLVIVVDEYGGTAGLVTMEDIVEEIVGEIRDEYDVEQELVLPVSENEAVMDARVPF
                     EDVRETFDLDVAPSEDYDTLGGFITNQLGRFPRAGEQVEAAGVRFVVETVEGRRIRRV
                     RVTRLVRSEEPA"
     gene            1386..1790
                     /gene="cdd"
                     /locus_tag="VFV20_07370"
     CDS             1386..1790
                     /gene="cdd"
                     /locus_tag="VFV20_07370"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013680390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU5288205.1"
                     /translation="MTLGPGDEELIKVASLARQRAHAPYSKYRVGAAIRTARNKVHAG
                     ANVENASYGLTICAERTAVFAAVNAGDTRLDTIAIVIDGDQLPSPCGACRQVLAEFAP
                     GMRIVLATTGGLRRETTLAELLPEPFLPENLR"
     gene            complement(1802..2503)
                     /locus_tag="VFV20_07375"
     CDS             complement(1802..2503)
                     /locus_tag="VFV20_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013127871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5288206.1"
                     /translation="MEQSVDPRPRVLVVDDDAGVVDVVCRGLAGAGFQVRPEVDGEGA
                     LRAIDRWMPDAMVLDVLMPGMDGLTVCRRARAADPGIGILMLTAKDGPMDEVLGLESG
                     ADDYVSKPFSMGVLVAHLRALLRRREPMAGGALVYEDLHLDTSAHVARRGDREIHLTA
                     TEYRLLHEFMRAPEQVLAKDLLTDRVWGYDFEGNHNVLEVYVRYLRQKIEPDGESRLI
                     HTLRGSGYVLRKTAP"
     gene            2805..3569
                     /locus_tag="VFV20_07380"
     CDS             2805..3569
                     /locus_tag="VFV20_07380"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU5288207.1"
                     /translation="MTTISRRRLLGGIGGGAAAFAAPNLIPFGFPQAEAATPSTKHQW
                     VVVIDMRRCDGCGVCTEACQAAHHLPKDHEWIKVEKKTTKSGQEFYMPRICMQCENAP
                     CIRVCPVGASFKDAEGVNLVDQNKCIGCRMCMAACPYGARYFNDEDPAPFNNPFGKPT
                     PQFPVPQQKGTVGKCMFCVHRTMAETGALPACVEGCNMFALYIGDLITDIATNGRETV
                     RLSQFLKDNDAWRYKEELHTGPRVYYISGHGQDLGF"
     gene            3570..>4369
                     /gene="nrfD"
                     /locus_tag="VFV20_07385"
     CDS             3570..>4369
                     /gene="nrfD"
                     /locus_tag="VFV20_07385"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEU5288208.1"
                     /translation="MDDRTQQLYRERAIANVGRTGPVWWAIAIALAGIVAAGVYAYTV
                     QVRYGHAVTGTNRTIFWGLYIANFVFFIGISYGGAITSAILRLTGAKWRAPITRIAEA
                     MAVVTLAVGALSVLIDVARPDRMLNLFIYAQPGSPITWDWIAINTYLVGTVIFFYLPL
                     IPDLGKLRDTLPANSLRRRLYAAVALRWEGTPAQERWLIRAVGIMAVVIIPLAVSVHS
                     VLAWLFAMTVREGWHSTIFGPQFVVAAMLSGVGMVILVIAAARKVYHL"
BASE COUNT          666 a         1521 c         1484 g          698 t
ORIGIN      
        1 tacgccggga tcgcggcggc gttcatcgcg ccgcgtctct ccgacatgct cgcgttcttc
       61 ctcggtatca gcaccatctt cagcgacgcg ggggcggtca cggtcaccgt ggcggtcctg
      121 tcgctcgtcg ccgtgctctc cgcgagcgag gcggcgctgc acagcgtgcg gcgcccggag
      181 atgatcgagg agctcgcgct gcgcggccgt cgcggacgcc gcgccggaac gatgggccgg
      241 cgacgcgcgc actacctcgc ggcgatccag gtgagcgagt tcctgatcgt gttcgcgtac
      301 gcgggcatcg cggcggcgtt catcgccccg cggctgtcgg cgctcctcaa gttccttctc
      361 gggatcgacg ccatcttcac cgacgtgagc gccgtcgtgg tcaccgtgtc cgtgctgtcg
      421 ctcgttgcgg tgctcttcgg cctgttcgtg ccgcgcgcca tcgccgcgcg ccacgcgcaa
      481 ggcgtcctgc tcggcctcgt ctggccgctg gaggtcgtga cctgggggac gcgccccctc
      541 gtctcgatgc tgttcctcct cacgcaggcc atcgcgcgcc cgttcggtgc gaacccccag
      601 gccggcacgc tcgtgaccga agaggagatc cgcgcgctcg tcgagacggg ccaggcgcag
      661 ggcgtcctcg agccgaagga gcgcgagatg atctcgtcga tcttcgagct cggcgagaag
      721 gaggtccgcg aggtgatgat cccgcgcacc gacctcgtcg ccctggacgt cacgacgccg
      781 atccgccagg tcctcgacga gatcacccgc atcggccatt cgcggctgcc ggtgtacgag
      841 ggatcggccg acgagatcgt cggcatcctc tacgtgaagg atctcttccg gaagctcgct
      901 cgcggtgagc gcgagctcga gctgcggcgc ctgctgcggc ccgtgcactt cgtccccgag
      961 acgaagaagg tcgacgatct gctgcgggag atgcagcggg acaagatgca cctcgtgatc
     1021 gtggtcgacg agtacggcgg caccgccggg ctcgtcacga tggaggacat cgtcgaggag
     1081 atcgtcggcg agatccgcga cgagtacgac gtcgagcagg agctcgtcct gcccgtgtcg
     1141 gagaacgagg ccgtcatgga cgcgcgcgtc cccttcgagg acgtgcgcga gaccttcgac
     1201 ctcgacgtcg cgccgtcgga ggattacgac acgctcggcg gattcatcac caaccagctc
     1261 ggccgctttc cgcgcgcggg cgagcaggtc gaggcggcgg gcgtgcgctt cgtggtggag
     1321 accgtcgagg gccggcgcat ccgccgcgtg cgggtcacgc gcctcgtgcg ctcggaggag
     1381 cccgcatgac gctcggtccc ggcgacgaag agctgatcaa ggtcgcgtcg ctcgcgcgcc
     1441 agcgtgccca cgcgccgtac tccaagtatc gcgtcggcgc ggccatccgc acggcacgga
     1501 acaaggtgca cgccggggcg aacgtcgaga acgcgtctta cgggctcacg atctgcgccg
     1561 agcgcaccgc ggtattcgcg gcggtgaacg cgggagacac gaggctcgac acgatcgcga
     1621 tcgtcatcga cggtgaccag ctgccgtcgc cgtgcggggc ctgccggcag gtgctggcgg
     1681 agttcgcgcc cggaatgcgc atcgtcctcg cgacgaccgg tgggctgcgc agagagacga
     1741 ccctcgccga gctgctgccg gagccgttcc tgcccgagaa cctgcgctag cgaggcgtcg
     1801 tttagggcgc ggtcttccgc agcacgtagc ccgatccgcg cagcgtgtgg atgagccggc
     1861 tctcgccgtc gggctcgatc ttctggcgca ggtagcggac gtacacctcg agcacgttgt
     1921 ggttgccctc gaagtcgtag ccccacacgc ggtccgtgag gaggtccttc gcgagcacct
     1981 gctccggtgc gcgcatgaat tcgtggagga gccggtactc cgtggccgtg aggtggatct
     2041 cgcggtcccc gcgccgtgcg acgtgcgcgg acgtatcgag gtgaaggtcc tcgtacacga
     2101 gcgcgccgcc ggccatcggc tcacgcctgc ggaggagcgc gcgcaggtgc gcgacgagga
     2161 cgcccatgct gaacggcttg ctgacgtagt cgtccgcgcc cgactcgagg ccgagcacct
     2221 cgtccatcgg cccgtccttc gccgtgagca tcaggatccc gatccccgga tcggccgcgc
     2281 gcgcgcgccg gcagacggtg aggccgtcca tgccgggcat gagcacgtcg agcaccatcg
     2341 catcgggcat ccagcgatcg atcgcgcgca gcgcgccttc accatcgacc tcgggacgca
     2401 cctggaaccc ggcgcccgcg agaccgcggc acaccacgtc cacgaccccg gcgtcgtcgt
     2461 ccaccacgag gacgcgcgga cgggggtcca cggactgttc cacgggtcac atcctcgccg
     2521 agagagcgcg atccgcgaag ggtccagagc cccatctcag tcgggccaaa ggttcctatc
     2581 ctcagccgcc gctcatgtac tcgccgcgcg gacgctcgat ggaccttagg actggctgaa
     2641 ggacatcacg aatcgcatga cagcgacgcc tgctccgcat tacgcggttc gtgccattcg
     2701 tgactatcac tcagcgtggt ctcaccgcat cgtcacggac caagagggcg ctgagagcga
     2761 cgccaagcga tcgctgagag cgcgtgcggt gaggagtgac aacggtgacc acgatctcgc
     2821 gccggcgtct ccttggcggt atcgggggcg gggcggctgc gttcgcggcc ccgaatctca
     2881 ttcccttcgg cttcccccag gcggaggcgg ccacgccgtc gacgaagcac caatgggtcg
     2941 tcgtcatcga catgcgccgc tgcgacggct gcggcgtctg caccgaagcg tgccaggcag
     3001 cgcaccacct gcccaaggac cacgagtgga tcaaggtcga gaagaagacg acgaagagcg
     3061 ggcaggagtt ctacatgccg cggatctgca tgcagtgcga gaacgcgccg tgcatccgcg
     3121 tctgccccgt cggcgcgtct ttcaaggacg ccgagggcgt gaacctcgtg gatcagaaca
     3181 agtgcatcgg ctgccgcatg tgcatggcgg cgtgcccgta cggcgcgcgc tacttcaacg
     3241 acgaggatcc cgcgcctttc aacaacccgt tcggcaagcc gacgccgcag ttccccgtcc
     3301 cgcagcagaa ggggaccgtg gggaagtgca tgttctgcgt ccaccgcacg atggcggaga
     3361 ccggcgcgct tcccgcgtgc gtcgagggct gcaacatgtt cgccctctac atcggcgacc
     3421 tcatcaccga catcgcgacc aacggccgtg agacggtccg cctctcgcaa ttcctgaagg
     3481 acaacgacgc gtggcggtac aaggaggagc tccacacggg gccccgcgtg tactacatca
     3541 gcggccacgg tcaggacctg gggttctaga tggacgaccg aacgcagcag ctctatcgcg
     3601 agcgcgccat cgccaacgtc gggcggaccg gaccggtctg gtgggccatc gcgatcgcgc
     3661 tcgccggcat cgtcgccgcg ggcgtctacg cctacacggt ccaggtgcgc tacgggcacg
     3721 cggtcaccgg caccaaccgc acgatcttct ggggcctcta catcgcgaac ttcgtgttct
     3781 tcatcggcat cagctacggc ggcgcgatca cgtcggccat cctgcgcctg accggcgcga
     3841 agtggcgcgc gccgatcacg cgcatcgcgg aggcgatggc cgtggtgacc ctcgcggtcg
     3901 gcgcgctctc ggtcctcatc gacgtcgcgc ggcccgaccg gatgctgaac ctgttcatct
     3961 acgcgcagcc cggctccccg atcacgtggg actggatcgc gatcaacacg tacctcgtcg
     4021 gcacggtgat cttcttctac ctgccgctga tccccgacct gggcaagctc cgcgacaccc
     4081 tcccggccaa cagtctccgt cgccggctct acgccgccgt cgcgctccgc tgggagggga
     4141 cccccgcgca ggagcggtgg ctcatccgcg cggtggggat catggccgtg gtcatcatcc
     4201 cgctcgcggt ttccgtgcac tcggtcctcg cgtggctgtt cgcgatgacc gtgcgcgagg
     4261 gttggcacag caccatcttc ggcccgcagt tcgtcgtcgc cgcgatgttg tcgggggtgg
     4321 ggatggtgat cctcgtgatc gccgcggcgc gcaaggtcta ccacctcga
//
LOCUS       DASTZR010000331         4384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_226727, whole genome shotgun sequence.
ACCESSION   DASTZR010000331 DASTZR010000000
VERSION     DASTZR010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226727"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="VFV20_07390"
     CDS             <1..721
                     /locus_tag="VFV20_07390"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEU5288209.1"
                     /translation="FVRGLIVLYESVSYGMRMLMRSADVQLPAESRQGTSSGATNTIA
                     MGAALVTALVVFIGIPYVVTQALHGAIESSVWLNVTEGLFRLALFLGYIAAISFLPDI
                     RRVFAYHGAEHMTIHAFEHGDSLTPERIEPYPTAHPRCGTAFLLLVVFISIVLFAFVP
                     RVNVVVDVFTRLLLVVPVASISYEALRLGARYEGHPLVALLVAPGLLLQKITTRRPDH
                     GMIEVAVASLEEAIAGDAEAA"
     gene            795..1901
                     /gene="prfA"
                     /locus_tag="VFV20_07395"
     CDS             795..1901
                     /gene="prfA"
                     /locus_tag="VFV20_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEU5288210.1"
                     /translation="MSELLTPGLRKRLAQIEERYDELARLMSDPATTSDRDRIRAVGQ
                     ELASLTPIVETVRELRAAEARMREAQELIDSDDGDLRDLAREELASAATEVDVIVARL
                     RVHLIPRDPLDEKNVIVEIRGGEGGEEAALFAADLYRMYSRYAERHRWKIEILSQSDS
                     EKGGFKEIIFRVAGRSAYSKLKFESGVHRVQRVPATEAQGRIHTSTATVAVLPEAEEM
                     DLAIDEADLKIDVYRAGGHGGQGVNTTDSAVRITHIPTGIVVTCQDERSQLKNKAKAM
                     AVLRARLLAAEQERRETEQGDARRAQIGRGERSEKMRTYNFPQDRITDKRLERNLSNI
                     ERRMDGDIDDLIEELSNQDEAERLARLEDDAADD"
     gene            1928..2395
                     /locus_tag="VFV20_07400"
     CDS             1928..2395
                     /locus_tag="VFV20_07400"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5288211.1"
                     /translation="MERLPYDDDTAFTLADDASTVARIVRTADRDRLRATRFDEWTAL
                     EVIGHVADMADVFAERVRRIVTEERPRLPSVDQDRIAAERRNNERDPMALARRIQAAH
                     AEIVSLLADPANRARPGIHEELGEVDAAFVAAYQARHAHEHVSDLAAAFPPTR"
     gene            2441..3961
                     /locus_tag="VFV20_07405"
     CDS             2441..3961
                     /locus_tag="VFV20_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288212.1"
                     /translation="MDAIRAQALAPLSRADAERLLPELATGRQTLERRVLRARCLKYL
                     ESFDLAWAELNAVLPLIKDPLLHARVAVDLLHLSYYLVRRDDSMRFKAIAEGDAAADP
                     IVLAELYLGSSIVNTAANELRVALTDARRADDALAVAPRGRGRDLVTTRVQRQLAHLL
                     SHAGDYVGARAAADATVRHAQRLNDPAEAAWATYTVGFADWFAGRLDGAVDAFTKAEA
                     ELRQYGSSVWRHTLLCLARAKLERGELTEGERLARQSATGAPEDHGHIALLRGETEVA
                     ELILSRTPKGMPEDEQFRDFVRALVIGKQGDPRKAVRLLEQATREFEARGMDHWAIGA
                     AVHAAFWRESVVRGGGASRAASLVRDIGTRGGEGFAYYLPEVGTWLGRAAERDPQARA
                     LGHTVRARAEAAERRAKADAGEGAGASALDEATFGLRAMGLTWREIGILREMEQLSRE
                     GRRLDRDALAGRLSVSPNTLRVHLTRIRAKLDVVDKRGDEVLLEAALSRRPGLVAP"
     gene            complement(4019..>4384)
                     /locus_tag="VFV20_07410"
     CDS             complement(4019..>4384)
                     /locus_tag="VFV20_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011751563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288213.1"
                     /translation="EAAGERLTSLLRDHIVIAGRLLGAAKTGDGTAFATAKADWYANG
                     HDIAAFLAAANPNWPLADLDAAMKMHLDTTLDEAVARLGGDWSADVVAYDHVHAHILR
                     MADVLSDGIIAQFPASFAK"
BASE COUNT          657 a         1578 c         1542 g          607 t
ORIGIN      
        1 gttcgtgcgc gggctcatcg tgctctacga gtcggtctcc tacggcatgc gcatgctcat
       61 gcgctccgcc gacgtccagc tgccggccga gtcgcggcag ggcacatcga gcggtgcgac
      121 gaacaccatc gccatgggtg ccgcgctcgt gacggcgctc gtcgtgttca tcggcatccc
      181 gtacgtcgtc acgcaggcgc tccacggggc gatcgaatcg agcgtttggc tcaacgtcac
      241 cgagggtctc ttccgcctcg cgctcttcct cggctacatc gccgcgatct ccttcctgcc
      301 cgacatccgc cgcgtcttcg cgtaccacgg cgccgagcac atgaccatcc acgcgttcga
      361 gcacggcgat tcactcacgc ccgaacgcat cgagccgtat cccacggcgc atccgcggtg
      421 cggcacggcg ttcctgctgc tcgtcgtgtt catctcgatc gtgctcttcg ccttcgtgcc
      481 gcgggtcaac gtcgtcgtcg acgtgttcac gcgcctgctg ctggtggtgc cggtcgcgtc
      541 catctcgtac gaggcgctcc gcctcggtgc ccgctatgag ggccacccgc tcgtcgccct
      601 cctcgtcgcg cccggcctcc tgctccagaa gatcacgacc cgccgccccg accacggcat
      661 gatcgaggtc gccgtcgcat cgctcgagga ggccatcgcc ggcgacgcgg aggccgcatg
      721 agcaaagccg agcgacgagc ggcttcgccg cgaggagcga gtcaaccagc gagcgaagcg
      781 agcgcgaaga ggaaatgagc gagttgctca cgcctggcct gcgcaagcgg ctcgcgcaga
      841 tcgaggagcg ctacgacgag ctcgcacggc tcatgtccga tcccgccacg acctccgacc
      901 gcgaccggat ccgcgcggtg gggcaggagc tcgcgagcct cacgccgatc gtcgagacgg
      961 tgcgcgagct gcgcgccgcc gaggcgcgca tgcgggaggc gcaggagctg atcgactcgg
     1021 acgacggcga cctgcgcgac ctcgcccgcg aggagctggc gtccgccgcg acggaggtcg
     1081 acgtcatcgt ggcgcggctg cgcgtgcatc tgatcccccg cgatccgctc gacgagaaga
     1141 acgtcatcgt cgagatccgc ggcggcgagg ggggcgagga ggccgcgctc ttcgcggccg
     1201 atctctaccg catgtactcg cgctacgcgg agcgccaccg ctggaagatc gagatcctct
     1261 cccagagcga ctcggagaag ggcggcttca aggagatcat cttccgggtc gcgggaagaa
     1321 gcgcgtacag caagctgaag ttcgagtccg gcgtgcaccg cgtgcagcgc gtgcccgcga
     1381 ccgaggcgca gggccgcatc cacacgtcga ccgcgacggt cgcggtgctc cccgaggcgg
     1441 aggagatgga cctcgccatc gacgaggcgg acctgaagat cgacgtgtat cgcgccggcg
     1501 gccatggtgg ccagggcgtg aacaccaccg actcggccgt gcgcatcacg cacataccga
     1561 ccggcatcgt cgtcacctgc caggacgagc ggtcgcagct gaagaacaag gcgaaggcca
     1621 tggccgtcct gcgcgcgcgg ctgctcgccg ctgaacagga gcgccgcgag acggagcagg
     1681 gcgacgcgcg tcgcgcgcag atcggccgcg gcgagcgttc ggagaagatg cgcacctaca
     1741 acttcccgca ggaccgcatc accgacaagc gtctcgagcg caacctctcg aacatcgagc
     1801 gccgcatgga cggcgacatc gacgacctca tcgaggagct ctcgaaccag gatgaggccg
     1861 agcgtctcgc gcggctcgag gacgacgccg ccgacgactg agccgcgccg cggtaacgtt
     1921 ccccgtcatg gaacgcctgc cgtacgacga tgacaccgcc ttcaccctcg ccgacgacgc
     1981 atccacggtc gcgcggatcg tccgcaccgc cgaccgcgac cgcctccgtg cgacgcgatt
     2041 cgacgagtgg accgccctcg aggtgatcgg ccacgttgcc gacatggccg acgtcttcgc
     2101 cgagcgcgtc cgccgcatcg tcacggaaga gcggccccgg ttgccgagcg tcgaccagga
     2161 caggatcgcc gcggagcggc ggaacaacga gcgcgacccc atggcgctgg cccgccgcat
     2221 ccaggcagcc cacgccgaga tcgtcagcct gctcgccgac cccgcgaacc gagcccgtcc
     2281 gggcatccac gaggagctcg gcgaggtgga cgcagcgttc gtcgccgcct accaggcccg
     2341 gcacgcgcac gagcatgtga gcgaccttgc ggccgccttc ccgccgaccc gctagcggtc
     2401 gccggcgaac gtaactcgta gtcgtggcgt aatacgttcg atggacgcca tccgggcgca
     2461 ggcgctcgcg ccgctgtcgc gcgccgacgc cgaacggctc ctgccggagc tcgcgacggg
     2521 gcggcagacg ctcgagcggc gagtgctgcg ggcgcgctgc ctcaagtacc tggagtcatt
     2581 cgacctcgcc tgggcggagc tgaacgcggt cctcccgctc atcaaggacc cgctcctcca
     2641 tgcgcgtgtc gccgtcgatc tcctccacct ctcctactac ctggtccggc gcgacgactc
     2701 gatgcggttc aaggccatcg ccgaagggga tgccgcggcc gacccgatcg tcctcgccga
     2761 gctgtatctc ggaagctcga tcgtgaacac ggcggcgaat gagctgcgcg tcgcgctcac
     2821 cgacgcccgt cgcgcggacg acgcgctcgc cgtcgcgccg cgcggccgcg gccgcgatct
     2881 cgtgacgaca cgcgtccagc gtcagctcgc gcatctgctg tcgcacgccg gcgactacgt
     2941 cggtgcgcgc gcggccgcgg acgccaccgt ccggcacgcc cagcgcctga acgatccggc
     3001 ggaagcggcg tgggcgacct acacggtcgg attcgccgac tggttcgcgg gccggctcga
     3061 cggcgccgtc gacgccttca ccaaggcgga ggccgagctg cggcagtacg gctcctcggt
     3121 gtggcggcac acgctgctct gcctcgctcg cgccaagctc gagcgcggtg agctcaccga
     3181 gggcgagcgg ctcgcgcggc agagcgcgac cggcgctccc gaggatcacg gccacatcgc
     3241 gctcctgcgc ggtgagaccg aggtcgcgga gctcatcctc tcgcgcacgc cgaaggggat
     3301 gcccgaggac gagcagttcc gcgacttcgt gcgcgcgctc gtgatcggaa agcagggcga
     3361 cccgcgcaag gcggtgcgcc tgctcgagca ggcgacccgc gagttcgagg cacggggcat
     3421 ggaccactgg gccatcggcg ccgcggtgca cgccgcgttc tggcgcgagt cggtcgtgcg
     3481 cggcggcggc gcgtcccgtg ccgcgtcgct ggtgcgcgac atcggtacgc gcggcggcga
     3541 ggggttcgcg tactacctgc cggaggtcgg gacctggctc gggcgcgcgg cggagcgcga
     3601 tccgcaggcg cgcgccctcg ggcataccgt ccgcgcgcgt gccgaggcgg cggagcggcg
     3661 cgcgaaggcc gatgcgggcg agggggccgg cgcgtcggcg ctcgacgagg cgaccttcgg
     3721 cctgcgcgcg atgggcctca cctggcgtga gatcgggatc ctccgggaga tggagcagct
     3781 ctcgcgcgag ggaaggcgcc tcgaccgcga cgccctcgcc ggacgcctct cggtctcgcc
     3841 gaacacgctg cgtgtccacc tcacgcgcat ccgcgccaag ctcgacgtgg tcgacaagcg
     3901 cggcgacgag gtgctgctcg aggccgcgct gtcgcgacgt ccggggctgg tcgctcccta
     3961 gacgcgcgac gagcccgccg tgaacgggcg ggctcgtcag cgtggaggag gggaagggtt
     4021 acttcgcgaa agaagcgggg aactgggcga tgatgccgtc gctcaggacg tccgccatgc
     4081 gcaggatgtg ggcgtgcacg tggtcgtacg cgacgacgtc ggcgctccaa tccccgccga
     4141 ggcgcgccac ggcctcgtca agggtcgtgt cgaggtgcat cttcatcgcc gcgtcgagat
     4201 cggcgagcgg ccagttcggg ttcgccgcgg cgaggaaggc cgcgatgtcg tggccgttgg
     4261 cataccagtc cgccttcgcg gtggcgaagg ccgtcccgtc accggtcttc gcggcgccca
     4321 gcaggcgtcc cgcgatcacg atgtggtcgc gcaggagtga cgtgagccgt tcgccggccg
     4381 cctc
//
LOCUS       DASTZR010000332         2317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_959097, whole genome shotgun sequence.
ACCESSION   DASTZR010000332 DASTZR010000000
VERSION     DASTZR010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959097"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VFV20_07415"
     CDS             <1..607
                     /locus_tag="VFV20_07415"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEU5288214.1"
                     /translation="NGLGNYEALRDAVGEQRAAVGVIYVGARLDERGELHATGPGRAE
                     LGRPRGAAPRRVQGALAEAMRAGGMSVTVVDDAWPAVWRKLVTNAAVNPTTALLGVLN
                     AQLMEDPASARIADRLAREVARVATAAGVPIADDDAVTWWREMAQLTGANRSSMLQDV
                     EAKRPTEVDAICGAVYREGQRRGVEAPLNQAMALLVSALHP"
     gene            complement(564..1745)
                     /locus_tag="VFV20_07420"
     CDS             complement(564..1745)
                     /locus_tag="VFV20_07420"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5288215.1"
                     /translation="MTRRTLLAGLSAAHFAQHVTNSLLSALLPFIRDAFLLSNTQAGF
                     AVTAYTIASGVTNAPLGVLADRIGARRVIVGGLVLVGLSSVAIGLAGSYEWLLIGLVV
                     MGVASATYHAPASALIAETFTFARRGVALGTHTTAGHLAFFAAPLLAGTLAFSGTWRL
                     PYVTFAIAPLACALLLLRIAPPGTRATERHEWLATFTDIGRVARSVGSLVSISIVAQV
                     LLSSALAFLTLYLVDARGVSPVVAAALFGVPQLAGLVAAPLSGVLSDRFGRPAVLLGA
                     LVASGPASWLLVAMPIELAVIPLLLIGACLSFRATATEVLIIDNTPVTRRSTVLGTYY
                     LVNQPVGGVAAPIFGAIADAAGIGSAFLWLSFAFVALSVVALVAAATGGARRRVAPSP
                     G"
     gene            complement(1742..>2317)
                     /locus_tag="VFV20_07425"
     CDS             complement(1742..>2317)
                     /locus_tag="VFV20_07425"
                     /inference="COORDINATES: protein motif:HMM:NF014611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEU5288216.1"
                     /translation="MYPGGARATLAPAEIAEPLEGRARPGHFAGVVTVVARLFDVVRP
                     DAAYFGQKDFQQLRVIQVTLGPRYPGLRIIGCPTVREPDGLAMSSRNAYLTREQRRDA
                     LALSGGLFAARDAWERGERHPVRLANAVRRHVDRPDTSVRLEYVSAADPFTLAELRAP
                     ADRIVLSLAARVGKARLIDNVLLGMDVSALS"
BASE COUNT          373 a          809 c          897 g          238 t
ORIGIN      
        1 gaacggcctc gggaactacg aggcgctgcg cgacgcggtc ggcgagcagc gggccgcggt
       61 cggcgtgatc tacgtgggcg cgcgcctcga cgagcgcggt gagctccacg ccacggggcc
      121 gggacgcgcc gagctcggcc ggccacgcgg tgccgcgccg cgccgggtcc agggcgcgct
      181 tgccgaggcg atgcgcgcgg gcggcatgag cgtcacggtc gtcgacgacg cgtggcccgc
      241 ggtctggcgc aagctcgtga cgaacgccgc ggtgaatccg accaccgcgc tgctgggcgt
      301 cctcaacgcg cagctgatgg aggatccggc ttcggcgcgc atcgccgacc gcctcgcgcg
      361 tgaggtcgcg cgggtcgcga cggcggccgg cgtcccgatc gccgacgacg atgccgtgac
      421 gtggtggcgg gagatggccc agctcaccgg cgcgaaccgg agctcgatgc tccaggacgt
      481 cgaggcgaag cgcccgaccg aggtcgacgc catctgcggc gccgtgtatc gcgagggtca
      541 gcgccgcggc gtggaggcgc cgctcaacca ggcgatggcg ctactcgtct ccgcgctcca
      601 cccgtagccg ccgcgacgag cgcgacgacc gagagcgcga cgaaggcgaa cgacagccac
      661 aggaacgcgc tgccgatgcc cgccgcgtcc gcgatcgcgc cgaagatcgg cgccgcgacc
      721 ccgccgacgg gctgattcac caggtagtag gtgccgagga cggtcgaccg ccgcgtcacc
      781 ggcgtgttgt cgatgatgag gacctcggtc gcggtggcgc ggaacgacag gcaggcgccg
      841 atgagaagca gtgggatgac ggcgagctcg atcggcatcg ccacgaggag ccacgacgcc
      901 ggtccgctgg cgacgagcgc gccgagcagc accgcggggc gtccgaagcg gtcggagagc
      961 acgcccgaga gcggtgccgc gacgagcccg gcgagctggg ggacgccgaa gagcgcggcc
     1021 gcgaccaccg gcgagacacc acgcgcgtcc accaggtaca gcgtcaggaa cgcgagcgcg
     1081 ctcgacagca gcacctgcgc gacgatcgag atcgagacga gcgagcccac cgaccgcgcg
     1141 acccggccga tgtcggtgaa cgtggcgagc cattcgtgcc gctccgtcgc gcgcgtccca
     1201 ggtggagcga tgcggaggag cagcagcgcg cacgcgagcg gcgcgatggc gaaggtcacg
     1261 tacggcagcc gccaggtccc ggagaacgcc agcgtgcccg cgagcagcgg agcggcgaag
     1321 aacgcgaggt gcccggccgt ggtgtgcgtg ccgagcgcga cgccgcggcg cgcgaaggtg
     1381 aaggtctccg cgatgagcgc cgacgccggc gcgtggtacg tcgcgctggc cacgcccatg
     1441 accacgagac cgatcagcag ccactcgtag gagcccgcga ggccgatggc gacgctcgaa
     1501 agaccgacga gcaccaggcc gccgacgatc acgcgccgag cgccgatgcg gtcggcgagc
     1561 acgcccagcg gcgcgttggt cacacccgac gcgatcgtgt acgcggtgac cgcgaagccg
     1621 gcctgcgtgt tcgacagcag gaacgcgtcg cggatgaacg gcagcagcgc cgagagcagg
     1681 ctgttggtga cgtgctgtgc gaagtgcgcg gcggaaagcc ccgcaaggag cgtccgccgc
     1741 gtcacgagag cgcgctcacg tccataccca ggaggacatt gtcgatgagg cgcgccttgc
     1801 ccacacgcgc cgccagcgac agcacgatgc ggtccgccgg cgcgcggagc tcggccagcg
     1861 tgaacgggtc ggcggccgag acgtactcga gacgcaccga cgtgtccggc cggtcaacgt
     1921 gtcggcgcac cgcgttcgcc agccgcacgg gatggcgctc gccgcgctcc cacgcgtcgc
     1981 gcgccgcgaa gaggcccccc gagagcgcga gcgcgtcgcg ccgctgctcc cgcgtgagat
     2041 aggcgttgcg ggaggacatc gcgagaccgt cgggctcgcg cacggtgggg cagccgatga
     2101 tgcgcagccc ggggtagcgc gggccaagcg tcacctggat cacgcggagc tgctggaagt
     2161 ccttctgtcc gaagtacgcg gcgtcgggcc gcacgacgtc gaacaggcgc gcgacgaccg
     2221 tgacgacgcc cgcgaagtgg ccgggacgcg cgcgcccctc gaggggctcg gcgatctccg
     2281 cgggtgcgag ggtcgcgcgc gcgccgcccg ggtacat
//
LOCUS       DASTZR010000333         3007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_824025, whole genome shotgun sequence.
ACCESSION   DASTZR010000333 DASTZR010000000
VERSION     DASTZR010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824025"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VFV20_07430"
     CDS             complement(<1..462)
                     /locus_tag="VFV20_07430"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEU5288217.1"
                     /translation="MTRLARLYLALFVANALAVAAVWWYGLEPTTPAGPAAVLNAVGR
                     LTALVGTYLLLVQLVLRTHVPWLVSAVGKDRLRTAHTWNAYAAFGLIGTHVVTQIVGY
                     AVQDRVDLVNEVALLVLHYDGVVTAIVGFALLAGLTIVSLERFRHRIPWPTW"
     gene            complement(459..863)
                     /locus_tag="VFV20_07435"
     CDS             complement(459..863)
                     /locus_tag="VFV20_07435"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEU5288218.1"
                     /translation="MTVGYADDLVAEARRAHGEGTLASPRRTAHSANPVCGDEIDLDL
                     DDEDGRIVNIAHRTRGCVFTRASASLLARAVEGRSLTVARELADILRRELAGSRALPA
                     ELAALESVRVYPARLRCALLPWDALRLALREA"
     gene            complement(860..1318)
                     /locus_tag="VFV20_07440"
     CDS             complement(860..1318)
                     /locus_tag="VFV20_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288219.1"
                     /translation="MRLVLTLLVAVVASACAPTAAPTPTSAAAPAKDVTILASDGGVM
                     RPIANGGRVPLRGGWATITLSPVPLQETAQLKVSVFDAEGRLAEANVSVDYASIDMDH
                     GHTLERGVLHENCYQMPLSFAMPGSWRLLIHVVRGPTDDTVTLVLPDVGL"
     gene            complement(1315..2406)
                     /locus_tag="VFV20_07445"
     CDS             complement(1315..2406)
                     /locus_tag="VFV20_07445"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEU5288220.1"
                     /translation="MDIQAILATPAYFRMTGQPGLAERYAASGQIVVILSQDIHYGEL
                     APVPTPPLLIDGQPYTPASTDTVFDSDHHRTVVLTYGDASRPLAAASVRTAELSMPGA
                     TGDLLWEHPFGVAGGAAAGGLSIPLLLALMGGMLASMWPCLFQLTAYFLPSVAGLSFD
                     QAREGRADGAVLRTAILFVSGIVIVYTLAGLAAGFAAQSVSGTAAFQAARQPLTFVAG
                     AIIIGMALRLALRARRPLVCHMPTTSPKPRRGFGTVALGLAFATGCMTCFGAAVVLGM
                     FTYVFTSASPFVGAAILFVFSLGIAVPLVVAAMAMARVLPLLTRLERVAPALTLLSAG
                     IMAAYGVLLLTGTSHVMSDTIARVANTLR"
     gene            2399..2647
                     /locus_tag="VFV20_07450"
     CDS             2399..2647
                     /locus_tag="VFV20_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288221.1"
                     /translation="MSMLPSSATARRSTSWMMLWAEGVAGAGAAPPLPTRIAIDASAA
                     ASSPAISSSVIGPRGLDAARAAIELTYFTVTVTGGTSR"
     gene            complement(2608..>3007)
                     /locus_tag="VFV20_07455"
     CDS             complement(2608..>3007)
                     /locus_tag="VFV20_07455"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU5288222.1"
                     /translation="AACGGASSAPTPAPQGTTAAAPTTAATGAASAPAASAATGPARK
                     VTVTATNSLYSISTLEVKAGEKIEFVLTNTGDEKHNMVAVGQDVSLVSPDFDAGQTLS
                     WTWTAPLKAGTFKLQCSYHRDVPPVTVTVK"
BASE COUNT          480 a         1011 c         1125 g          391 t
ORIGIN      
        1 ccacgttggc catgggatgc ggtgccggaa tcgctcgagc gagacgatgg tgagtcccgc
       61 gagcagcgcg aatccgacga tggcggtgac gaccccgtcg taatgcagga cgagcagagc
      121 gacttcgttg accaggtcga cgcggtcctg gacggcgtac ccgacgatct gcgtcacgac
      181 gtgcgtcccg atgagcccga acgccgcgta cgcattccag gtgtgtgccg tacgcagccg
      241 gtcctttcct accgcgctga cgagccacgg tacgtgcgtc cgcaggacga gctgcacgag
      301 cagcagatag gtgccgacga gcgcggtcag cctgccgacg gcgttgagca ccgcggcggg
      361 gcccgccggc gtcgtgggct cgagcccgta ccaccagacg gcggcgacgg cgagcgcgtt
      421 cgcgacgaac agcgcgaggt acagccgcgc gagccgggtc acgcctcgcg gagcgcgagg
      481 cggagcgcgt cccatggcag gagcgcgcag cgcaaccgtg cgggatagac gcgcaccgac
      541 tcgagcgcgg cgagctccgc gggcagcgca cgcgagccgg cgagctcgcg ccgcaggatg
      601 tcggcgagct cgcgcgcgac ggtcagagag cgtccctcga cggcgcgcgc cagcagcgac
      661 gccgaggcgc gcgtgaacac gcagccgcgc gtccggtggg cgatattcac gatccgtcca
      721 tcctcgtcgt cgagatcgag gtcgatctcg tccccgcaca ccggattcgc gctgtgcgcc
      781 gtacgtcgtg gcgatgcgag ggtgccttcg ccgtgcgcgc ggcgcgcctc cgcgacgagg
      841 tcgtccgcgt agcccacggt cacagcccga cgtccgggag cacgagcgtc acggtgtcgt
      901 cggtcggccc gcggacgaca tggatcagaa ggcgccacga gccgggcatg gcgaaggaca
      961 gcggcatctg atagcagttc tcgtgcagga cgccacgctc gagggtgtgg ccgtgatcca
     1021 tgtcgatcga tgcgtagtcc acggacacat tggcctcggc cagccgcccc tcggcgtcga
     1081 acaccgagac cttcagttgt gcggtctcct gcagcggaac gggcgagagc gtgatcgtcg
     1141 cccagccgcc gcgaaggggc acgcgaccgc cgttggcgat aggacgcatg acgcctccgt
     1201 cgctcgcgag gatcgtgacg tccttcgccg gcgccgcggc ggacgttggc gtcggcgcag
     1261 cggtgggcgc gcacgcgctc gcgacgacag cgacgaggag cgtgagaacg agcctcatcg
     1321 cagggtgttc gcaacacggg cgatcgtgtc gctcatgacg tgtgaggtgc ccgtgaggag
     1381 cagcacgccg tacgcggcca tgatcccggc gctgagcagg gtcagggcgg gagccacgcg
     1441 ctcgaggcgc gtgagcagcg ggagcacgcg cgccatcgcc atcgcagcaa cgacgagcgg
     1501 taccgcgatg ccgagcgaga acacgaagag gatcgcggca ccgacgaacg gcgatgccga
     1561 cgtgaagacg tatgtgaaca tgcccagcac gacggccgcg ccgaagcagg tcatgcaccc
     1621 cgtcgcgaac gcgaggccga gcgcgacggt gccgaagccg cggcgaggct tcggcgaggt
     1681 cgtgggcatg tgacagacga gcggacgccg cgcgcgcagc gccagccgca gcgccatgcc
     1741 gatgatgatg gcgccggcga cgaaggtcag cggctgccgc gccgcctgga atgccgcggt
     1801 gccggacacg gactgtgcgg cgaaccccgc ggcgagaccg gcgagggtgt acacgatcac
     1861 gatgccggac acgaacagga tcgccgtccg gagcaccgcg ccgtcggcgc gtccttcgcg
     1921 cgcctgatcg aacgacaggc ccgcgacgct cggaaggaag taggccgtca gttggaagag
     1981 acacggccac atcgacgcga gcatcccgcc catgagcgcg aggagcaatg ggatcgagag
     2041 gccgcccgcg gcggccccac cggcaacgcc gaacgggtgc tcccacaaga ggtcaccggt
     2101 cgcgcccggc atgctgagct ccgccgtgcg cacgctcgcg gcagcgagcg gacgcgaggc
     2161 gtcgccgtac gtgagcacga cggtccggtg gtgatcgctg tcgaacacgg tgtccgtgct
     2221 cgccggcgtg tacggctgcc cgtcgatgag cagcggcggc gttgggaccg gcgcgagctc
     2281 gccgtaatgg atgtcctgcg agaggatgac cacgatctgc ccgctcgcgg catagcgctc
     2341 ggcgagtccg ggctgtccgg tcatgcggaa gtacgcgggc gtcgcgagga tcgcttggat
     2401 gtccatgctg ccgtcgtcgg cgaccgcgcg tcgctcgacg tcctggatga tgctctgggc
     2461 ggagggcgtc gccggcgccg gtgcggcgcc gccgctcccg accaggatcg cgatcgatgc
     2521 gagcgcggcc gcgagcagcc cggcgatcag cagcagcgtg atcgggcccc ggggcctgga
     2581 cgcggcgcgc gccgcgatcg agctaaccta ttttaccgtc acggtgaccg gggggacgtc
     2641 ccggtgatac gagcactgca gcttgaacgt gcccgccttc agcggcgccg tccaggtcca
     2701 cgagagcgtc tggcccgcgt cgaagtcggg actgacgagg gagacgtcct ggccgaccgc
     2761 gaccatgttg tgcttctcgt caccggtgtt cgtcagcacg aactcgatct tctcgcccgc
     2821 cttcacctcc agggtcgaga tgctgtagag gctgttggtc gcggtgacgg tgaccttgcg
     2881 cgccgggccg gtggccgcgg aagccgcagg tgccgatgcg gcgcccgtcg cggcggtcgt
     2941 gggcgcggcg gcggtcgtgc cctgcggcgc gggcgtgggc gcggatgacg ctcccccaca
     3001 cgccgcg
//
LOCUS       DASTZR010000334         2511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_354313, whole genome shotgun sequence.
ACCESSION   DASTZR010000334 DASTZR010000000
VERSION     DASTZR010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354313"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1360
                     /gene="glyA"
                     /locus_tag="VFV20_07460"
     CDS             101..1360
                     /gene="glyA"
                     /locus_tag="VFV20_07460"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010099448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU5288223.1"
                     /translation="MTELQRVDPEIYEAIENEKRRQVEKVELIASENYTSAAVMEAVG
                     SVLTNKYAEGYPGHRYYGGCEWVDVAESLAIERAKSLFGAQHANVQPHAGAQANMAAY
                     AAVLQPGDTVLGLALDQGGHLTHGSPVNFSAQLYHFVPYGVRKEDERIDYDALEGIAK
                     EHKPKLIVAGATAYPRFFDFARMRRIADEVGALLLVDMAHFAGLVAAGEHPSPIPHAQ
                     IVTTTTHKTLRGPRGGMILCDEQFAKAVDKAVFPYAQGGPLMHVIAGKAVMLKEAATP
                     EFKRYAAQIRRNATILADSLAKAGLRIVSGGTDNHLMLVDVRPLDLTGKQAEKALDAV
                     GITVNKNTIPYDPQKPGTASGIRVGTPAVTTRGMREAEMERLGALIARALRAVGDETV
                     AKDVASEVLDLTRRFPVPGITTAAAAR"
     gene            1408..1989
                     /gene="upp"
                     /locus_tag="VFV20_07465"
     CDS             1408..1989
                     /gene="upp"
                     /locus_tag="VFV20_07465"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEU5288224.1"
                     /translation="MTRLRDERTDPRDFRALVAEIATLLLYEATEDLPMQETQVKTPL
                     ATAKGVRLAEPIGLVPVLRAGIGMVEAALELMPDAQVWHIGLFRDERTLRPIEYYNKL
                     PSEATVTVCLVLDPMLATGGSAVATIDILKKWGAKRVKYVGIIAAPEGVSALTTAHPD
                     VPIHVAALDERLNEKGYIVPGLGDAGDRQFGTG"
     gene            2048..>2511
                     /locus_tag="VFV20_07470"
     CDS             2048..>2511
                     /locus_tag="VFV20_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288225.1"
                     /translation="MIARLALLFAVVSLVACGPNARGASPTPTATARTASGAPSASPS
                     PVPAEYASIYRSIQGQLDLWDRSLPPERATRVPVFGGHVLASNGNRGADLLASETMPT
                     VDLSLDAMKRLGAKGVSVTISFPLFNASWPRANDYIAFYETVAKHVRDRGLVL"
BASE COUNT          413 a          879 c          884 g          335 t
ORIGIN      
        1 agggcggccg ccacgagcgg cggcggaagc agatcgaggc gatggagccg cgaccgatga
       61 cgaccgcgga cgccacgcag ggcatgcgaa ggggtgtgaa gtgacggagc tacagcgggt
      121 cgacccggag atctacgagg cgatcgagaa cgagaagcgc cgccaggtcg agaaggtcga
      181 gctcatcgcc agcgagaact acaccagcgc cgcggtcatg gaggccgtcg ggagcgtcct
      241 cacgaacaag tacgccgagg gctacccggg gcatcgctac tacggtggct gtgagtgggt
      301 ggacgtcgcg gagtcgctcg ccatcgagcg agcgaagtcg ctcttcggcg cgcagcacgc
      361 gaacgtgcag ccgcacgccg gcgcgcaggc gaacatggcc gcgtacgccg cggtcctgca
      421 gccgggcgat acggtgctcg gactcgcgct ggaccagggc ggtcacctca cgcacggctc
      481 gccggtgaac ttctccgcgc agctctacca cttcgtgccg tacggggtcc gaaaggagga
      541 cgagcgcatc gactacgacg cgctcgaggg gatcgcgaag gagcacaagc cgaagctcat
      601 cgtcgccggc gcgaccgcgt acccgcgctt cttcgacttc gcgcgcatgc ggaggatcgc
      661 ggacgaggtc ggcgcgctcc tgctcgtgga catggcgcac ttcgccgggc tcgtcgcggc
      721 gggggagcat ccctcgccga tcccgcacgc gcagatcgtc acgacgacga cgcacaagac
      781 gctgcgcggt ccgcgcggcg gcatgatcct ctgcgacgag cagttcgcga aagccgtgga
      841 caaggccgtg ttcccgtacg cccagggcgg cccgctcatg cacgtcatcg ccggcaaggc
      901 ggtcatgctc aaggaggccg cgacccccga gttcaagcgc tacgccgcgc agatccgcag
      961 gaacgcgacg atcctcgcgg actcgctcgc gaaggccggg ctgcgcatcg tgtcgggcgg
     1021 gaccgacaac cacctgatgc tggtcgacgt tcgcccgctc gacctgaccg gaaagcaggc
     1081 cgagaaggcg ctcgacgccg tcggcatcac cgtgaacaag aacacgatcc cgtacgaccc
     1141 gcagaagccg gggaccgcgt cgggcatccg tgtgggtacg cccgcggtca cgacgcgcgg
     1201 catgcgcgag gcggagatgg agcggctcgg cgcgctcatc gcgcgcgcac tgcgggcggt
     1261 cggcgacgag acggtcgcga aggacgtcgc gtccgaggtg ctcgacctca cgcggcgctt
     1321 cccggtgccg gggatcacca ccgccgcggc tgcgcgctga gcacaccgcc gctccacgtt
     1381 tctacgcacc cgctgctgcg ggagaaggtg acgcgcctgc gcgacgagcg caccgacccg
     1441 cgcgacttcc gcgcgctcgt cgcggagatc gcgacgctgc tcctgtacga ggcgaccgag
     1501 gacctgccga tgcaggaaac gcaggtgaag acgccgctcg cgacggcgaa gggcgtgcgg
     1561 ctcgccgagc cgatcggtct cgtgccggtg ctgcgcgcgg ggatcggcat ggtggaggcc
     1621 gcgctcgagc tcatgccgga cgcgcaggtc tggcacatcg gtctcttccg cgacgagcgc
     1681 acgctgcggc cgatcgagta ctacaacaag ctgccgtcgg aggcgaccgt caccgtgtgc
     1741 ctcgtgctcg acccgatgct cgcgaccggc ggctccgccg tcgcgaccat cgacatcctg
     1801 aagaagtggg gcgcgaagcg cgtgaaatac gtcgggatca tcgccgcccc cgagggtgtg
     1861 agcgcgctca cgacggcgca ccccgacgtg cccatccacg tcgccgcgct cgacgagcgt
     1921 ctcaacgaga agggctacat cgttcccggc ctcggcgacg ccggcgaccg gcagttcggg
     1981 accggatagc ggccgcgaat ttaccggccg ctgacaacat cgccggccgg tggtgagtat
     2041 cagggtcatg atcgcccggc tcgcgctgct cttcgccgtc gtgtcgctcg tcgcgtgcgg
     2101 tccgaacgcg cgcggcgcgt cgccgacgcc caccgccacg gcgcgcacag cgtccggcgc
     2161 acccagcgcc tcgccgtcgc cggtccctgc cgagtacgcc tcgatctaca ggagcatcca
     2221 ggggcagctc gatctctggg accgctcgct ccccccggag cgcgcgactc gcgtgcccgt
     2281 cttcggaggg cacgtcctgg cctcgaacgg caatcgcggc gcagacctcc tcgcctcgga
     2341 gacaatgccg accgtcgacc tgtcgctcga cgcgatgaag cggctcggtg cgaagggcgt
     2401 cagcgtgacg atctcgttcc cgttgttcaa cgcgtcgtgg ccgcgcgcga acgactacat
     2461 cgcgttctac gagacggtcg cgaagcacgt gcgcgatcga gggctcgtgc t
//
LOCUS       DASTZR010000335         2151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_254372, whole genome shotgun sequence.
ACCESSION   DASTZR010000335 DASTZR010000000
VERSION     DASTZR010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254372"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFV20_07475"
     CDS             complement(<1..183)
                     /locus_tag="VFV20_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015329732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288226.1"
                     /translation="MSSPASSSATGELAGERALIERIRQRVPPAPAALIIGIGDDAAV
                     IAPDRGALQVLTTDALV"
     gene            complement(167..1744)
                     /gene="rny"
                     /locus_tag="VFV20_07480"
     CDS             complement(167..1744)
                     /gene="rny"
                     /locus_tag="VFV20_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEU5288227.1"
                     /translation="MGLSWGLAAVLAITAAVSAAGLFFWTLTKGRRTATEAMAAARAE
                     AERIRLQAERDAESLRKEAALEAREKAHELAAETERLSRERRQEITALEQALADKTRA
                     LADRIAATDRIEQDLRAREKDVAAQQERAKAAAVRSEQIVADRQRELQRVAGLTADEA
                     RELLLKQIEADARRDAANLVKRLEAEARETAAQRAQGIITDAIQRSAAEHAIETTVSV
                     VDLPSDDLKGRIIGREGRNIRALETATGVELIVDDTPGAIILSSFDPFRREVARQAIA
                     RLIADGRIHPARIEEVVEKVKAEMEETVRREGEAAAFELGLYDVHPEVLKLMGRLKFR
                     TSYGQNVLNHSKEVAFLAGIMAKEVGLNSHVAVRAAFLHDCGKAIDRDLQGTHLELGL
                     EFLRKHGESEPVLQAVAAHHMDIDWPSLEAMIVQAADAISAARPGARRDILESYVKRL
                     EKLEGIADSFEGVSKSFALQAGREIRIVVESERISDEATVWLSKDIARRIENELEYPG
                     QIKVTVIRETRAVEFAR"
     gene            complement(1816..1999)
                     /gene="ssrS"
                     /locus_tag="VFV20_07485"
     ncRNA           complement(1816..1999)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VFV20_07485"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
BASE COUNT          298 a          733 c          732 g          388 t
ORIGIN      
        1 gacgagtgca tcggtcgtca gcacctgcag ggccccgcga tccggcgcga taaccgccgc
       61 atcgtcgccg ataccgatga tcagtgcggc tggcgcgggg ggcacgcgct ggcggatacg
      121 ctcgatcagg gcgcgctcgc cggccagttc acctgtggcg gagctgctag cgggcgaact
      181 caacggccct cgtctcccgg atgacggtga ccttgatttg tcccgggtat tcgagctcgt
      241 tctcgatgcg gcgggcgatg tccttcgaca gccagaccgt cgcctcgtcc gagatccgct
      301 cgctttcgac gacgatgcgg atttcgcggc cggcctggag ggcgaacgat ttggagacgc
      361 cttcgaagga gtcggcgatg ccttccagtt tctcgagccg cttcacgtac gattcgagaa
      421 tgtcgcgacg ggccccggga cgcgcggcgg aaatggcgtc cgccgcctgg acgatcatcg
      481 cctccagcga cggccagtcg atgtccatgt ggtgtgctgc gaccgcctgc agcacgggct
      541 cgctctcgcc gtgcttgcgc aggaactcga gcccgagctc gagatgggtc ccctggaggt
      601 cgcggtcgat cgccttgccg caatcgtgca ggaacgcggc gcgcacggcg acgtggctgt
      661 tgagaccgac ttccttcgcc atgatgccgg cgaggaaggc gacctccttc gagtggttca
      721 gcacgttctg tccgtagctg gtgcggaact tcaggcgtcc catcagcttc aggacttccg
      781 ggtggacgtc gtacagcccc agctcgaacg ccgccgcttc tccctcgcgg cggaccgttt
      841 cctccatctc cgccttcacc ttctcgacga cctcttcgat gcgggcgggg tggatgcgtc
      901 cgtcggcgat gagccgcgcg atcgcctgcc gggcgacctc gcgccggaac ggatcgaagc
      961 tcgagaggat gatggcgccc ggcgtgtcat cgacgatcag ctcgacgccg gtggccgttt
     1021 cgagcgcgcg gatgttccgc ccctcccggc cgatgatgcg ccctttcaaa tcgtcgctcg
     1081 gcagatcgac gaccgacacg gtcgtttcga tcgcgtgttc ggcggcgctg cgctggatgg
     1141 cgtcggtgat gatgccctgc gcgcgctgcg cggcggtctc gcgcgcttcg gcctcgaggc
     1201 gcttgaccag gttggcggca tcccgccggg catccgcctc gatctgcttg aggagcagct
     1261 cgcgggcttc gtccgccgtc agtccggcaa cgcgctggag ctcgcgctgc cggtccgcga
     1321 cgatctgctc gctgcggacg gccgccgcct tggcgcgctc ctgctgggcg gcgacgtcct
     1381 tctcgcgcgc gcgcaggtcc tgctcgatcc ggtcggtcgc ggcgatgcgg tccgcgagcg
     1441 cgcgcgtctt gtcggcgagc gcctgctcga gggcggtgat ctcctgccgc cgttcgcgcg
     1501 acagccgctc ggtctcggcc gcgagctcgt gggccttctc ccgcgcttcg agcgccgctt
     1561 ccttgcgcag cgactccgcg tctcgttcag cctgcagccg gatgcgctcc gcctcggcgc
     1621 gcgcggcggc catcgcctcg gtggcggtcc gacgaccctt ggtgagggtc cagaagaaca
     1681 ggcccgccgc ggacacggcg gccgtgatgg ctagaacagc agccagaccc caagatagcc
     1741 ccatgcaggc tctccataag cccgggctcc gggagccggg cggtctcgct cgtgacggtc
     1801 agattgtgag gaaggaaagg ccccgctttg ccgtgtgggg gggtcggatg aacctgcata
     1861 agcaggtgga accgctgcac aagccgcgtt tcaggcttcc tcttgcagag gcatgcacac
     1921 cacgcagcgt cgcgattctc ttagggtgaa acgttggctc aaacgagccc ccgaaccatc
     1981 acgcgcaaag cagggaactc aaactcctgg ctgaagtgta cttcagccgc acccgttcgt
     2041 cgaggtcatc ttgcaagggc ctcgggctcg ctgatcccga gggcaaggtc gagcatccgc
     2101 tcgagttcac cggcgcggtc ggcgagcagg gcctggccgc gctgctgttc g
//
LOCUS       DASTZR010000336         5592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_40731, whole genome shotgun sequence.
ACCESSION   DASTZR010000336 DASTZR010000000
VERSION     DASTZR010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40731"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3305)
                     /locus_tag="VFV20_07490"
     CDS             complement(<1..3305)
                     /locus_tag="VFV20_07490"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEU5288228.1"
                     /translation="MAQGGPARRPDVHAAMFWCATVLEGGLAALIVVSPSLFESPIYA
                     ALKPVLAPLAILCLAGSAASLSAVTGLLSRRTQATAAILSTLPLVVFTLGFAITGAVT
                     GVITYGIEVAALGVTAWLIVAGRSPQARVYVVMVGAILAAQGFTMALAPWSFANATVY
                     AELGQYLPIVAPAFILGGVGLVISELRRAPGRRVTVSVLAFAVFCAADLAALVSVFAV
                     TGAWTGVLSYGLLAVILLLAVPRTSLVAESPILFAALAALAGAGDLVAGTLVQAGGPQ
                     LAAPWAIMRPLTALGLAFIATAFALTQHVGRPAPRLAATLTIVGLACAVAQAAVTLAV
                     GAAWGPAALLTGAEETGAAVSTIAGPAGVILVATVLIVVLVRPHGRIVPAVFVLSGSM
                     IAGTVGLNILGFMIRSPFLFDIYGALGMRSHATVAFGALALALLAAGIGRLLAAPIGD
                     RLFGTIGALTVLVLLRGYISDAALTHVFTDTADVGNAEYLAAVDAARQLALVMLVGVA
                     VGAGLVLTRTVTLPLRALLDAIARARAGESRAQAEVSGEDEIGIVARSFNQLTRELAD
                     QADLYTAVRRAQGELGEAILILEEGQPRTWNEALATISGYTPADLHAMPSILDLWPSS
                     ERDDFARLLRARAHESYRTETTIRRRSGAPADLELAVHPLRDTARPQQLAIMRDITES
                     KQARRSLERLALHDALTGLANRALFADRLERTIGAATRTNDTFALLYLDLDRFKEVND
                     AFGHDAGDELLRELSQRISRPLTSADTLARFGGDEFAILLPHARDVVDATAMAEVVRA
                     VLARPFDLLDHRIFVEASIGVVLFPRDGGDADTLLRHADIAMYQAKHTGKGIIAYATE
                     HDPQSQHRLDLMSDLRHAIERDELRLHFQPQVRLHGEGACTSLEALVRWQHPARGMVA
                     PASFIPLAENSGFINELGLWVIREGTRALGLLPSSGIPMRLAINLSARNLHYTGLVDA
                     VGSALRAARLDPSALTIEITETAVMSDAQRSLETLARLSALGVRISIDDFGTGYSSLA
                     YLRRLPVNELKIDRTFVMDMLANASSDAIVTSVIGLGHSLGFSVVAEGVEDADTMSAL
                     SARGCDLAQGYHIA"
     gene            complement(3399..4298)
                     /locus_tag="VFV20_07495"
     CDS             complement(3399..4298)
                     /locus_tag="VFV20_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015036525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU5288229.1"
                     /translation="MRTDRFLAMVAVALGLVYVVWGSTYFAIAIMVQTLPPLVAAGAR
                     FFIAGLALAVWLAVRDRAALRVTRHQLASAALVGILLLAGGNGLVTLGERTVPSGLAA
                     LIVASVPLWIVLLRLGAGDRPHVDVVAGVTVGIVGVAVLVVPTGMSGAVDVAGLAMLV
                     GATLSWSVGTFISPRIRMPKVPLASTALQMLAAGVVLPLVAVPLGEYRAIDLAHASAQ
                     SVLAFAYLVVFGSIVAFSAYTWLLQHASVSLVSTYAYVNPVIAVFLGTLFLREPLTPT
                     MLAGAALILAAVAFIVTRTATRG"
     gene            4357..4818
                     /locus_tag="VFV20_07500"
     CDS             4357..4818
                     /locus_tag="VFV20_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018847888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HEU5288230.1"
                     /translation="MAATRQAKVVWNGDLAKGSGEVSGLSSDTFSGLPVSWASRTEEP
                     QGRTSPEELLAAAHASCYAMALSAGLAKAGTPPQRLEVTATIRFDKVLDAWAVASSEL
                     AVSATVPGVDEEAFTRIAQDAKDNCPISRALKGNVSISVTATLAERSSTRA"
     gene            complement(4819..>5592)
                     /locus_tag="VFV20_07505"
     CDS             complement(4819..>5592)
                     /locus_tag="VFV20_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU5288231.1"
                     /translation="TMPSKTQPTLGHIRGTAPANAAVSLTTSTGQSSRTQIADGRGWF
                     AFVDVPPGSYTVAASGALLGYALVSAGQIASVTTTPPAATPTPSPTPSPTPTPPPGAC
                     ANSVGPGIPPPAAVPAGIPGFHAAWWGQSGYMTLCPGGQLPATVAYKNSGARGWVSGR
                     MGEVAYLGTWSPEPGQDRASPLGGDGTLGSPNTGWPRYNRIAIQPAAYVGPDQVSWFQ
                     FTVQAPMTPGTYRLYLRPLVEGAQWMEDYGVYWLVTVRS"
BASE COUNT          919 a         1916 c         2070 g          687 t
ORIGIN      
        1 gcgatgtgat agccctgcgc gagatcgcag ccgcgggccg agagcgcgct catcgtgtcg
       61 gcgtcctcga ccccctcggc gaccaccgag aacccgaggg agtggccgag gccgatgacc
      121 gaggtcacga tggcgtcgct ggaggcgttc gcgagcatgt ccatcacgaa ggtgcgatcg
      181 atcttcagct cgttcaccgg cagccggcgc agatacgcga gcgacgaata cccggtgccg
      241 aagtcgtcga tcgagatccg gacgccgagc gcggagagtc gcgcgagcgt ctcgagcgac
      301 cgttgggcgt ccgacatcac cgccgtctcg gttatctcga tcgtcagcgc gctcgggtcg
      361 agccgtgcgg cgcggagcgc cgagccgacc gcgtcgacaa ggccggtgta atgcaggttg
      421 cgtgcggaca ggttgatcgc gagccgcatc gggatcccgg acgacggcag cagccccagc
      481 gctcgcgtgc cttcgcggat cacccagagc ccgagctcgt tgatgaagcc agagttctcg
      541 gcgaggggga tgaagctcgc gggcgcgacc atgccgcgcg cgggatgctg ccaccgcacg
      601 agcgcttcga gcgacgtgca cgcgccctcg ccgtggagcc ggacctgcgg ctggaagtgc
      661 agccgcagct cgtcgcgctc gatcgcgtgg cgcaggtcgc tcatgagatc gaggcgatgc
      721 tggctctgcg ggtcgtgctc ggtcgcgtac gcgatgatgc ccttgccggt gtgcttcgcc
      781 tgatacatcg cgatgtccgc gtgacgaagg agggtgtcgg cgtccccgcc gtcccgcggg
      841 aagagcacga cgccgatgct tgcctcgacg aagatgcgat ggtccagcag gtcgaatggc
      901 cgcgcgagca ccgcgcggac gacctcggcc atcgcggtcg cgtcgaccac atcgcgagcg
      961 tgggggagga ggatcgcgaa ctcgtcacca ccgaaccgcg cgagcgtgtc ggcggaggtg
     1021 agcgggcgcg agatccgctg gctcagctca cggagcagct cgtctccggc gtcgtgcccg
     1081 aacgcgtcgt tgacctcctt gaagcggtcg aggtcgagat agagcagcgc gaacgtgtca
     1141 ttcgtgcgcg tggcggcacc gatggtccgc tcgagtcgat cggcgaagag cgcacgattc
     1201 gcgagccccg tgagcgcgtc gtgcagcgcg agccgctcga gcgagcggcg agcctgcttc
     1261 gactcggtga tgtcgcgcat gatcgcgagc tgctgcggac gagcggtgtc gcggagcggg
     1321 tggacggcaa gctcgaggtc cgcgggtgcg ccactgcgcc ggcggatcgt cgtctcggtg
     1381 cggtatgact cgtgcgctcg cgcgcgcagc agtctcgcga agtcgtcgcg ctcggatgac
     1441 ggccagagat cgaggatgga cggcatcgcg tgcaggtccg ccggcgtgta cccgctgatc
     1501 gtcgcgagcg cctcgttcca cgtccgcggc tgaccctcct cgaggatgag gatggcctcg
     1561 ccgagctcgc cctgcgcacg gcgcaccgcg gtgtagagat ccgcctggtc ggccagctct
     1621 cgcgtcagct ggttgaagga acgggccacg atgccgatct cgtcttcgcc gctgacctcg
     1681 gcctgcgcgc gtgactcacc ggcgcgtgcc cgtgcgatcg cgtcgaggag ggcgcgcagc
     1741 gggagcgtca cggtgcgggt gagcaccagg ccggcgccga ccgcgacgcc gacgagcatg
     1801 acgagcgcca gctggcgcgc cgcgtcgacc gcggcgaggt actcggcgtt cccgacgtcc
     1861 gcggtgtcgg tgaaaacgtg cgtcagggcc gcgtcggaga tgtagccgcg cagcaggacg
     1921 aggaccgtga gggcgccgat ggtgccgaag agacggtctc cgatcggcgc ggcgaggagc
     1981 cggccgatgc ccgccgcgag gagtgcgaga gcgagcgcgc cgaacgcgac ggtcgcatgc
     2041 gaccgcatgc ccagcgcacc gtagatgtcg aagaggaacg gcgagcggat catgaagccg
     2101 aggatgttga gcccgaccgt tcccgcgatc atcgaaccgg acagcacgaa cacggcgggc
     2161 acgatgcggc cgtgcggtcg cacgaggacg acgatgagga cggtggcgac gaggatgact
     2221 ccggccgggc cggcgatcgt cgagaccgcg gcgccggtct cctctgcgcc cgtcagcagt
     2281 gcggccggtc cccacgcggc gccgacggcg agcgtgaccg cggcctgcgc gaccgcgcac
     2341 gcgaggccga cgatcgtcag cgtcgcggcg agccgcggcg cgggccgacc gacgtgctgg
     2401 gtgagcgcga aggctgtggc gatgaacgcg agcccgaggg ccgtgagcgg gcgcatgatc
     2461 gcccacggtg ccgcgagctg cggcccaccg gcctgcacca gcgtgcctgc gacgagatcc
     2521 cccgcgcccg ccagtgccgc gagcgccgcg aagaggatcg gcgactccgc gacgagcgat
     2581 gtccgcggca ccgccaggag aaggatcacc gcaagcaggc cgtatgacag gacgccggtc
     2641 cacgcgcccg tgaccgcgaa caccgacacc agcgcggcaa ggtccgcagc gcagaagacc
     2701 gcgaacgcga ggaccgaaac ggtgacgcgg cggcccggag ctcgtcgaag ctcgctgatg
     2761 acgagcccga cgccccccag gatgaatgcc ggcgccacga tcggcaggta ctgtccgagc
     2821 tccgcgtaga ccgtggcgtt ggcgaacgac cacggcgcga gcgccatcgt gaagccctgc
     2881 gccgcgagga tcgctccgac catcacgacg tacacgcggg cctgcgggga gcgaccggcg
     2941 acgatgagcc acgccgtcac gcccagcgcc gcgacctcga tgccgtaggt gatcacgcct
     3001 gtgaccgccc ccgtgatcgc gaacccgagc gtgaagacga cgagcggcag cgtcgagagg
     3061 atggccgcgg tcgcctgcgt ccggcgactg aggagtcccg tcaccgccga cagggacgcg
     3121 gcagatccgg ccaggcagag gatcgcgagc ggcgcgagca ccggcttcag cgcggcgtag
     3181 atcgggctct cgaagagcga aggcgacacg acgatgagcg ccgccagccc gccttcgagc
     3241 acggtggcgc accagaacat cgcggcatgg acgtcgggcc gtcgcgccgg ccccccttga
     3301 gccatcccgg acaaccctcc tgtcgggcgg gggcctacag caagggccga acggcccggt
     3361 tggacactac gaacgccggg cgccccggtc ctctccgctc aaccgcgcgt cgcggtccgg
     3421 gtcacgatga acgccacggc ggcgaggatg agcgccgcac ccgcgagcat cgtcggggtg
     3481 agcggctccc gcaggaagag cgtgccgagg aacacggcga tcaccggatt cacgtaggcg
     3541 taggtcgaca cgagcgagac cgaggcgtgc tgtagcagcc aggtatacgc ggagaacgcg
     3601 acgatcgagc cgaacacgac gaggtacgcg aacgcgagga ccgactgcgc cgatgcgtgc
     3661 gcgagatcga tcgcccggta ctcgccgagc ggcaccgcga cgagcggcag gacgacgccc
     3721 gcggcaagca tctggagcgc ggtcgatgcg agcggcacct tcggcatgcg gatgcgcggc
     3781 gagatgaacg tgccgaccga ccacgagagc gtcgcgccga cgagcatggc gagaccggcc
     3841 acgtctaccg cgcccgacat tcccgtcggc acgacgagga ccgcgacgcc gacgatgccg
     3901 acggtcacgc cggcgacgac gtccacgtgc gggcgatcac cggcgccgag gcgaaggagc
     3961 acgatccaga gcggcacgga cgccacgatg agcgcggcga ggcccgacgg tacggtgcgc
     4021 tcgcccagcg tgacgagtcc gttgccgccc gcgagcagca ggatgccgac gagcgccgcg
     4081 ctcgcgagct ggtggcgcgt gacgcgcagt gcggcgcggt cgcgcacggc cagccagacg
     4141 gcgagcgcga ggccggcgat gaagaaccgc gcgcccgccg cgacgagcgg cggcagggtc
     4201 tggaccatga tcgcgatcgc gaaatacgtg gacccccaca ccacgtacac gagccccagc
     4261 gccaccgcga ccatcgcgag aaacctatcg gttcgcatcc cggcgcgttc tatgcagccc
     4321 cctccggcag cacatccgca tcggaaggag cgagccatgg cggcgacacg acaagccaag
     4381 gtggtctgga atggcgacct cgcgaagggg agcggcgagg tgagcggact ctccagcgac
     4441 accttctcgg ggctcccggt ctcgtgggca tcccgcaccg aggagccgca gggccgcacg
     4501 agtcccgagg agctcctcgc cgcggcccac gcgagctgct acgcgatggc gctctccgct
     4561 ggtctcgcga aggccggcac gccaccgcag cggctcgagg tgacggcgac catccgcttc
     4621 gacaaggtgc tggacgcgtg ggccgtcgca tcgagcgagc tcgcggtcag cgcgacggtg
     4681 cccggcgtcg acgaggaggc attcacgcgg atcgcgcagg acgcgaagga caactgcccg
     4741 atcagcaggg ccttgaaggg gaacgtctcg atcagcgtga cggcgacgct tgccgagcgc
     4801 tcgtcgacgc gagcctgatc agctcctgac ggtcacgagc cagtacacgc cgtagtcctc
     4861 catccactgc gcgccctcga cgagcggtcg caggtagagg cggtacgtgc ccggtgtcat
     4921 cggcgcctgc acggtgaact ggaaccacga gacctggtcg ggcccgacgt acgcggcggg
     4981 ctggatcgcg atgcggttgt atcgcggcca gccggtgttc ggcgatccca gcgtgccgtc
     5041 gccgccgagc ggcgacgccc tgtcctgccc cggctcgggt gaccacgtgc ccagatacgc
     5101 gacctcgccc atgcgaccgg agacccagcc gcgcgcgccg ctgttcttgt acgcgaccgt
     5161 cgcgggcaac tgaccgccgg ggcagagcgt catgtagccg ctctgtcccc accacgcggc
     5221 atggaacccg gggatcccgg cggggaccgc tgccggcggc gggatgccgg gaccgacgct
     5281 gttcgcgcac gcgcccggcg gcggcgtcgg cgtgggtgag ggcgtgggcg acggcgtcgg
     5341 agtcgccgcg ggcggtgtcg tcgtgacgga cgcgatctga cccgcgctca ccagcgcgta
     5401 ccccagcagg gcgccgctcg cggccaccgt gtagctgccc ggcgggacgt ccacgaacgc
     5461 gaaccatccg cgaccgtcgg cgatttgcgt gcgggaggac tgcccggtcg aggtggtcaa
     5521 cgacaccgcc gcgttcgccg gcgccgtgcc gcggatgtgg ccgagcgtgg gctgcgtctt
     5581 cgacggcatc gt
//
LOCUS       DASTZR010000337         4394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_123795, whole genome shotgun sequence.
ACCESSION   DASTZR010000337 DASTZR010000000
VERSION     DASTZR010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123795"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..473)
                     /locus_tag="VFV20_07510"
     CDS             complement(108..473)
                     /locus_tag="VFV20_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288232.1"
                     /translation="MSDTTKLIPPPRRGPRWVDLGLAAVAGSLLTVLALGIVFFGRAG
                     VAPANAASSSPSASLASLSASPSISIRSATVAPVTAVPTTAAPTTAAPTPPPATTAPA
                     PTPPPAATTAPPPLPTKTP"
     gene            complement(508..762)
                     /locus_tag="VFV20_07515"
     CDS             complement(508..762)
                     /locus_tag="VFV20_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288233.1"
                     /translation="MWEPSAVGLKQIVNVLLAAIAGALGVLVVGEARETFPARVQSPS
                     PSISATATPRATLEEMRTAAPTAQRRDDRGGKGKDGKGGD"
     gene            complement(813..2840)
                     /locus_tag="VFV20_07520"
     CDS             complement(813..2840)
                     /locus_tag="VFV20_07520"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEU5288234.1"
                     /translation="MSMNAIAPARLLPGASVEQARGAERATAVGAKAIRGKALIFWDP
                     KVPGKKLDAIDTDQITPATDCVSESLDTLDARWKAGSFRFLMPDFRERVARGESFVVA
                     GDRFAIGSSREMSPAGLKGVGDDAGHEIVIVCGAGMGDIFRRNALNLGLHVVQSRAAV
                     DDAQEGDTFSFDPATRRLTNETRGTSYEPTPLSASEEEIRRSGGIIKVGRREFAESVR
                     RTPLVAWPDATTARRLSSTDQILWAHRVDKDAEVRPGATIRVYADLLPASDGTGPFAI
                     HTFNQISGGDTIYPRQVAYANDHFVFNHREADDKQTAIGKQFADHYKIGRPRYATPGD
                     GIFHFYFPEQGLVVPGALIPGADSHSRAYGAYGALGYGVGSTTLGFGWATGYVYFTCV
                     KQRRVRFTGKLNPWVGGKDVVLALLARWGAKQSQGMSVEFVDADRQLPMSYRNTIANM
                     MAEGEATNGIFAPDETTLGWYREKRIALPYPAFAPGDDVPWEMDEELDLSQVEPLIAK
                     PFSPANAFPASEVAKERLAFDKAFIGSCTNGGYGDLLEAALVIAAAKRKGATKAAREF
                     VVFPGSGGVKKDIERGDPRLGGESIADVFRSVGAEIRESWCGPCFGQGPDALVKGETA
                     ITSFNRNWANRMGVGGMGYLASPAVVAASALLGYMGPPSDLGIEWHAERFV"
     gene            complement(2837..3529)
                     /locus_tag="VFV20_07525"
     CDS             complement(2837..3529)
                     /locus_tag="VFV20_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5288235.1"
                     /translation="MTGSIRTLIVDDDYRVAEVHRGFIELVPSFDVVGIAHTAAEALE
                     LARSERPDLVLLDVYLPDRSGLDVLRELRASGDPPVDVIAITAANDVETLRSALQGGV
                     VHYLVKPFQFNAFREKLESYAALRSRLGGAREVDQREIDELYALLRTRADAPELPKGL
                     SPATLALVARTVREAAGDMSALEVAQATGMSRVTSRRYLEHLARSGLIEVTIRYGTTG
                     RPEHRFRATMKA"
     gene            complement(3526..>4394)
                     /locus_tag="VFV20_07530"
     CDS             complement(3526..>4394)
                     /locus_tag="VFV20_07530"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5288236.1"
                     /translation="TVANDEARRLLGITGAVEGRRLAEVVPEGRAREVLGGSTTGNDQ
                     VVLAGDRVLVLNRMPVAVRGRDVGAVVTLRDRTELEGVLRELDTVRGLAQALRAQTHE
                     FSNKLHIVAGLIELGRLEEAVRFIAKTSLVHQELVELVQQRIADPALAALLLAKAALA
                     SERRVDLRVASDAHLPRDLADVRDLVTVVGNLVDNAIDAAAAAEDGWVEVSVRAQDTA
                     VVVRVRDSGPGIDPAVVDDIFRDGFTTKRDGSHHGVGLALVRQVTQRRGGWIRVASDG
                     GTVFTALIPNAE"
BASE COUNT          627 a         1541 c         1568 g          658 t
ORIGIN      
        1 acgcgggtct gcgccgcaat ggcggttccg ggtgtgcaca cggcaaacgt caggtgcgca
       61 actaaatcgt tagcgacccg gccctaacgc gtccgttcag cgcgcgttca cggtgtcttc
      121 gtcgggagcg gtggcggcgc cgtcgtcgcc gccgggggcg gtgtcggtgc cggcgcggtc
      181 gtggccggtg gcggtgtcgg ggcagcggtg gtcggtgccg cggtcgtcgg tacggcggtc
      241 accggggcga cggtcgcgct gcggatcgag atactgggcg acgccgagag cgacgcgagg
      301 ctcgccgacg gtgaggacga cgcggcgttc gccggagcga cgcccgcgcg gccgaagaag
      361 acgatcccga gcgcgagcac ggtgagcagg ctgccggcga cggccgcgag gcccaggtcg
      421 acccagcgcg ggccgcgtcg cggcggaggg atgagcttcg tcgtgtcgga catggcatga
      481 ggctgtcgca cgcctcatgc cgcccgctca gtcgcctccc tttccgtcct ttcccttgcc
      541 gccacggtcg tcacgcctct gcgcggtggg cgccgcggtg cgcatctcct ccagcgtggc
      601 acgtggggtg gccgtcgcgg agatggacgg cgacggagac tggacacgcg ccgggaaggt
      661 ctcacgcgct tcgccgacga cgagcacgcc gagcgctccc gcgatcgccg cgagcaggac
      721 gttgacgatc tgcttcaggc cgaccgccga gggttcccac atacagatgg aacgggcgag
      781 tcgcgtgccg cgatcagcgt gttacgggat cgttagacga aacgttcggc gtgccattcg
      841 atgcccaggt cgctcggcgg ccccatgtag ccgagcaggg ccgaggcggc caccacagcg
      901 ggcgacgcga ggtaccccat cccgccgacc cccatgcggt tcgcccagtt gcggttgaac
      961 gacgtgatcg ccgtttcacc cttgaccagc gcgtccggcc cctgcccgaa acacggcccg
     1021 caccacgatt cgcggatctc ggcgccgacc gaacggaaca cgtcggcgat cgattcgccg
     1081 ccgagccgcg gatcgccgcg ctcgatgtcc ttcttcacgc caccggagcc ggggaacacg
     1141 acgaattcgc gcgccgcctt cgttgcgccc ttccgtttcg cggcggcgat cacgagcgcg
     1201 gcctcgagca gatcgccgta gccgccgttc gtgcacgagc cgatgaaggc cttgtcgaac
     1261 gcgagacgct ccttcgcgac ctcggacgcg gggaacgcgt tggcgggcga gaagggcttc
     1321 gcgatgagcg gctccacctg cgagagatcg agctcctcat ccatctccca cggcacgtcg
     1381 tcgccgggcg cgaatgccgg gtaggggagc gcgatccgct tctcccgata ccacccgagc
     1441 gtcgtctcgt ccggtgcgaa gatgccgttc gtcgcctcgc cttcggccat catgttcgcg
     1501 atggtgttcc ggtagctcat gggcagctgc cgatcggcat cgacgaactc gacgctcatg
     1561 ccctgcgact gcttcgcgcc ccagcgcgcg agcagcgcga gcacgacgtc cttaccgccg
     1621 acccaggggt tcagcttccc ggtgaaacgg acgcggcgct gcttcacgca ggtgaagtac
     1681 acgtagccgg tggcccagcc gaagcccagc gtcgtggaac ccacgccgta gccgagcgcg
     1741 ccgtacgccc cgtacgcgcg gctgtgcgaa tccgctccgg ggatgagcgc gccgggcacg
     1801 acgagaccct gctccgggaa gtagaagtgg aagatcccgt cgccgggcgt ggcgtaccgc
     1861 ggcctgccga tcttgtagtg atcggcgaac tgcttgccga tcgcggtctg cttgtcgtcg
     1921 gcctcgcgat ggttgaagac gaagtggtcg ttcgcatacg cgacctgtcg cggatagatc
     1981 gtgtcgccgc cgctgatctg gttgaaggtg tggatcgcga acggtcccgt cccatcggac
     2041 gccgggagca gatccgcgta cacgcggatg gtcgcgccgg ggcgcacctc ggcgtccttg
     2101 tcgacgcggt gtgcccacag gatctggtcg gtggacgaca ggcgccgcgc ggtcgtcgcg
     2161 tccggccacg cgacaagagg cgtccgccgc acggactccg cgaactcgcg ccggccgacc
     2221 ttgatgatgc cgcccgaccg gcggatctcc tcctcgctcg cggacagcgg cgtcggctcg
     2281 tagctcgtgc cacgcgtctc attggtgagc cggcgggtcg ccgggtcgaa cgagaacgtg
     2341 tcgccctcct gcgcgtcgtc caccgcggcg cggctctgca cgacgtgcag acccaggttc
     2401 agcgcgttgc ggcggaagat gtcgcccatc cccgcgccgc acacgatgac gatctcgtgg
     2461 cccgcgtcgt cgcccacgcc cttcagaccc gcgggactca tctcccgcga cgatccgatc
     2521 gcgaagcggt cgccggccac gacgaagctc tcgccgcgcg ccacgcgctc gcggaagtcc
     2581 ggcatcagga agcggaacga tccggccttc cagcgcgcgt cgagcgtgtc gaggctctcg
     2641 gacacgcagt ccgtcgcggg cgtgatctgg tccgtgtcga tggcgtcgag cttctttccc
     2701 gggaccttgg ggtcccagaa gatcagcgcc ttgccgcgga tggccttcgc gccgacggcc
     2761 gtcgcccgct ccgctccgcg cgcctgctcg accgatgcgc cggggagaag ccgagcgggc
     2821 gcgatcgcgt tcatgctcat gccttcatgg tggcacggaa ccgatgctcg ggccggccgg
     2881 tggtgccgta gcggatcgtg acctcgataa ggcccgatcg tgcgagatgc tcgaggtagc
     2941 gccgcgaggt gacgcggctc atgcccgtcg cctgggcgac ctcgagggcc gacatgtcgc
     3001 cggccgcctc gcgcaccgtg cgcgccacga gtgcgagcgt cgcgggcgag agacccttcg
     3061 gcagctcggg tgcgtccgcg cgcgtccgca gcagcgcgta cagctcgtcg atctcccgct
     3121 gatcgacctc gcgcgcacca ccgagccgcg atcgcagcgc ggcgtagctc tcgagcttct
     3181 cgcggaacgc gttgaactgg aatggcttga cgaggtaatg cacgacgccg ccctggagcg
     3241 ccgaccgcag cgtctcgacg tcgttcgcgg cggtgatcgc gatgacgtcc accggcgggt
     3301 cgcctgacgc gcgcagctcg cgcagcacgt cgaggccgga gcgatcgggg agatagacgt
     3361 cgaggagcac gagatcgggg cgctcgctcc gcgccagctc cagcgcctcg gccgcggtgt
     3421 gggcgatgcc gaccacgtcg aacgacggta cgagctcgat gaagccgcgg tgtacctcgg
     3481 cgacacggta atcgtcgtcg acgatcaggg tgcggatgct gcccgtcatt ccgcgttcgg
     3541 gatgagcgcc gtgaagaccg tgccgccgtc gctcgcgacc cggatccatc cgccgcggcg
     3601 ctgcgtgacc tggcggacga gcgcgaggcc gaccccgtgg tgcgagccgt cgcgcttggt
     3661 cgtgaagcca tcacggaaga tgtcgtcgac gactgccgga tcgatgccgg gcccggagtc
     3721 gcggacgcgc acgacgacgg cggtgtcctg cgcgcgcacg ctgacctcga cccagccatc
     3781 ctcggccgcg gccgcggcgt cgatcgcgtt gtcgacgagg ttgccgacca cggtgacgag
     3841 atcgcggacg tcggcgaggt ctcgcggaag gtgcgcgtcg ctcgcgaccc ggaggtcgac
     3901 gcggcgttcc gacgcgagcg cggccttcgc gagcagcagc gccgcgagcg cgggatcggc
     3961 gatgcgctgc tgcacgagct cgacaagctc ctgatggacg aggctcgtct tcgcgatgaa
     4021 ccgcacggcc tcctcgaggc gcccgagctc gatgagcccg gcgacgatgt gcagcttgtt
     4081 cgagaactcg tgcgtctgcg cgcgcagcgc ctgcgccagg ccgcgcacgg tgtcgagctc
     4141 acgcaggacg ccctcgagct cagtgcggtc gcgcagggtc acgaccgcgc cgacatcgcg
     4201 gccgcgcacc gcgacgggca tgcgattgag cacgaggacg cggtcgccgg cgagcacgac
     4261 ctggtcgttg ccggtcgtcg agccgccgag gacctcgcgc gctcgcccct cgggtacgac
     4321 ctcggcgagc cggcggccct cgaccgcgcc cgtgatgccg agcaggcggc gcgcctcgtc
     4381 gttcgccacg gtca
//
LOCUS       DASTZR010000338         5507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_406591, whole genome shotgun sequence.
ACCESSION   DASTZR010000338 DASTZR010000000
VERSION     DASTZR010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5507
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406591"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VFV20_07535"
     CDS             complement(<1..300)
                     /locus_tag="VFV20_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5288237.1"
                     /translation="MSGANILFVDDEEQIRKLVSTYLARQGYAVVVKTDGYEALKAIR
                     ANTPDLVITDVMMPNMNGFELTRRLRADHRTARIPVLMLSARKEADDVLTGYSEGA"
     gene            complement(297..668)
                     /locus_tag="VFV20_07540"
     CDS             complement(297..668)
                     /locus_tag="VFV20_07540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5288238.1"
                     /translation="MGTVVFVEDDATIRKLVQVALKSTAHAVHFASNGREGLALVERV
                     RPDVVFTDVAMPEMDGLQLADALHARPDLADIPIVFVTASLQREQIERYFAHGARTYL
                     AKPFSTAELRAQVDALVGAKL"
     gene            complement(674..3118)
                     /locus_tag="VFV20_07545"
     CDS             complement(674..3118)
                     /locus_tag="VFV20_07545"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5288239.1"
                     /translation="MGRFPRSLRLYVAASSIYAALVFLAVHAVYGVELGRAGVQLASL
                     DAVLSSIDLVSFLFWIGISLIAAVVLYRLAIALEGARDEGAQRQGELASIFALGQALS
                     GSLELDEIAERFLAAALSSTDPTVTAALYVDDETIDGYRLVLERGPGAGRLGESQYSA
                     SAVPAPIRTRVVDHRQSLVLSDTHASEAWAALAAGLRDANAVRSFAALPLVSHDRLVG
                     MVLFASDRAGAITADGLQLIALVTQFVAASVRTALSLREAQRRGDREVIVNRVAQRAR
                     ASLDPDEVVRATVRELAGALHVRRVIAAIGSRPDDLRVAYEWHAPDLPPLGVGSTDLP
                     AARQAALTGRTSVSHDEWSRIATPIVVGGETAGVLSLHDEIARDWTIDEVRLVEAVAR
                     EMRAAIEAARLFQSRQRENERLLALQRASTVLAARATTRDVIDEVLRTASSLLGQASA
                     SLYLWDESTGALRLAQNADPAARAVSAVLEPGHGAGDLLRRLEPVVVNEYAQWTGATK
                     LGIEAGCTALLAVPLVRSGQLLGAIVLRSYDGRTRFTLDDARLLALFGDQAVTALTNA
                     EAFDRQRRAMEELEKVNRAKSEFVSIVSHEFRTPLTGIQGFSEMMRDEDLSPTEMKEY
                     AADINKDAQRLNRLINDMLDLSRMEAGRMTLDRRPTDVNAIVSEVADRIRPAAPQHPI
                     TLELDPSLPRVAADHDRLIQVVSNLLSNAVKYSPTGGDVVVSTKREADGVHLRVRDHG
                     MGIPQESLERIWEEFSRVESRETKGIQGTGLGLPIVRQIVSMHGGRVWAESEVGRGST
                     FHVHLPLAATGEAVEA"
     gene            3259..3909
                     /locus_tag="VFV20_07550"
     CDS             3259..3909
                     /locus_tag="VFV20_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEU5288240.1"
                     /translation="MSATNYVPMVIEQSGRGERAYDIFSRLLKERIIFIGDAIEDTMA
                     NLIIAQLLFLESEDPERDIYLYINSPGGVVTAGLAIYDTMQYLKAPVSTICVGQAASM
                     GAVLLAAGTKGKRYALPNSRIMIHQGSGGFRGNTPDVMIQVKELETLVDKLMKIIAHH
                     SGQDYDKVKRDVDRDRFMSAEEAKAYGLIDDIFVGKESLISIAKAEEKERVPAGTR"
     gene            3906..5177
                     /gene="clpX"
                     /locus_tag="VFV20_07555"
     CDS             3906..5177
                     /gene="clpX"
                     /locus_tag="VFV20_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEU5288241.1"
                     /translation="MTTTAKGGKPYRCSFCGKSQDQVRKLIAGQGVYICDECITLCRE
                     IVDEEFMEAPKARTLGQKIPSPRRINEILDQYVIGQDLAKRVLSVAVYNHYKRVSAGH
                     RVDDVELGKSNVLLIGPTGCGKTLLAQTLARILDVPFSIADATSLTEAGYVGEDVENI
                     LLRLIQAADFDLNRAQRGIIYIDEIDKIARKGDNPSITRDVSGEGVQQALLKILEGTV
                     ANVPPQGGRKHPHQDFIQIDTTNILFICGGAFEGLEKIVEARVGKKTMGFGATLRDNT
                     KETSAVLQQVMPDDLLKYGLIPEFVGRLPVIVPLMALDENDLMRILTEPKNAIVKQYQ
                     KFLALDKVELQLTDEALRSAAAGAAKRKTGARGLRTIIEEVLLDVMYEIPSRNDVRRV
                     VVTPEAIDGTAKPLLLSKNEQLVLEGDEATA"
     gene            5266..>5507
                     /locus_tag="VFV20_07560"
     CDS             5266..>5507
                     /locus_tag="VFV20_07560"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5288242.1"
                     /translation="MIQFDLDPKVVVETHKHDVEQFGVVVKGSLAMVIAGEQRILTPG
                     DTYRIPPGAAHGARVFEEQTRVIDVWAPPRTDLLKK"
BASE COUNT          918 a         1902 c         1879 g          808 t
ORIGIN      
        1 ggcaccttcc gaatagccgg tcagcacgtc gtcggcttcc ttgcgcgccg agagcatgag
       61 gacggggatc cgcgcggtgc ggtgatcggc gcgcaggcgc cgggtgagct cgaacccgtt
      121 catgttgggc atcatcacgt cggtgattac gaggtcgggc gtgttcgcgc ggatcgcctt
      181 cagcgcctcg tacccgtcgg tcttcaccac gacggcgtag ccctgacgtg ccaggtaggt
      241 cgagacgagc ttgcggatct gttcctcgtc gtcgacgaag aggatgttcg ctccgctcac
      301 agcttcgctc cgacgagcgc gtctacctgc gcgcgcagct ctgcggtgct gaacggcttg
      361 gcgaggtacg tccgcgcgcc gtgggcgaag taccgctcga tctgctcccg ctgcagggac
      421 gcggtgacga agacgatggg gatgtccgcg aggtcgggtc gagcgtgcag agcgtcggcg
      481 agctgcagcc cgtccatctc cggcatcgcc acgtcggtga acaccacgtc cggccgcacg
      541 cgttcgacga gggcgagacc ctcgcggccg ttcgaagcga agtggaccgc gtgcgccgtg
      601 gacttgagcg cgacctggac gagcttgcgg atcgtcgcgt catcctcgac gaacacgacg
      661 gttcccatcg cggtcaagcc tctaccgctt cgcccgtcgc cgcaagcggg aggtgaacgt
      721 ggaacgtcga tccgcgaccg acctcgctct cggcccagac ccggccgccg tgcatgctca
      781 cgatctggcg gacgatgggc aggcccaggc cggtgccctg gatccccttc gtctctcgtg
      841 actcgacacg cgagaactcc tcccagatgc gctccagcga ctcctgcggg atgcccatgc
      901 cgtgatcccg caccctgaga tggacgccgt cggcctcgcg cttcgtcgag accacgacat
      961 cgccgccggt cggggagtac ttcaccgcgt tgctcaggag attgctgacg acctggatga
     1021 ggcgatcgtg gtcggcggcg acgcgtggca gagacgggtc gagctcgagc gtgatcggat
     1081 gctgcggcgc ggccggccgg atgcggtccg cgacctccga aacgatcgcg ttcacgtccg
     1141 tgggtcgccg gtcgagcgtc atccgtccgg cctccatgcg cgaaagatcc agcatgtcgt
     1201 tgatgagccg gttgaggcgt tgcgcgtcct tgttgatgtc cgccgcgtac tccttcatct
     1261 cggtgggcga cagatcctcg tcccgcatca tctcgctgaa gccctggatg cccgtgagcg
     1321 gcgtgcggaa ttcgtggctt acgatcgaca cgaactcgct cttcgcgcga ttcaccttct
     1381 cgagctcctc catcgcgcgc cgctggcggt cgaaggcctc agcgttggtc agcgcggtca
     1441 ccgcctggtc gccgaacagc gcgagcaggc gtgcgtcgtc gagggtgaat cgcgtccgcc
     1501 cgtcgtacga gcggagcacg atggcgccga gcagctgccc gctgcgcacg agcggcacgg
     1561 ccagcagcgc ggtgcatccc gcttcgatgc ccagcttcgt ggcgccggtc cactgcgcgt
     1621 actcgttcac gacgacaggc tcgagccgcc ggaggagatc gccggcgccg tgacccggct
     1681 ccagtaccgc gctgacggcg cgcgctgcgg gatcggcgtt ctgcgcgagc cgcagggcgc
     1741 cggtcgactc gtcccagagg tagagcgacg cgcttgcctg gccgaggagc gacgacgccg
     1801 tgcgcagcac ctcgtcgatg acgtcgcgcg tcgtcgcacg agccgcgagc acggtcgacg
     1861 cgcgctggag cgcgagcaga cgctcgttct cgcgctgccg cgactggaag aggcgcgccg
     1921 cctcgatggc cgcgcgcatc tcgcgcgcga ccgcctcgac gagtcggacc tcgtcgatcg
     1981 tccagtcgcg cgcgatctcg tcatggagcg acagcacgcc cgcggtctcg cctcccacga
     2041 cgatcggcgt cgcgatgcgg ctccactcgt cgtgcgagac cgacgtcctc cccgtgagcg
     2101 ccgcctgccg cgccgccggc agatcggtcg agccgacacc gagcggcggg aggtcgggcg
     2161 cgtgccattc gtacgcgacg cggaggtcgt cgggccgcga gccgatcgcg gcgatcacgc
     2221 gccgcacgtg cagcgccccg gcgagctccc ggaccgtcgc gcgaaccacc tcgtcgggat
     2281 cgagcgaggc gcgcgctcgc tgggccacgc gattgacgat cacctcacgg tccccacggc
     2341 gctgcgcctc gcgcagggac agcgcggtcc gcacggaggc ggcgacgaac tgcgtgacga
     2401 gcgcgatgag ctgcagcccg tccgccgtga tggcgccggc acgatcggag gcgaacagca
     2461 ccatgccgac gagccggtcg tgggacacga gcggcagcgc cgcgaacgag cgcacggcgt
     2521 tcgcgtcgcg gagtccggcc gcgagcgcgg cccaggcctc gctggcgtgc gtgtcgctga
     2581 ggacgagcga ttggcggtgg tccacgacgc gcgtgcggat cggggcgggc accgcgctcg
     2641 ccgagtactg cgactcgccg agccgcccgg cgccggggcc gcgctcgagc acgaggcggt
     2701 agccgtcgat ggtctcgtcg tccacgtaca gggccgcggt gaccgtgggg tcggtcgagg
     2761 agagcgcggc ggccaggaag cgctcggcga tctcgtcgag ctcgagcgaa ccggagaggg
     2821 cctggcccag cgcgaagatc gacgcgagct ctccctggcg ctgagctccc tcgtcgcgcg
     2881 ccccctcgag cgcgatcgcg aggcggtaca ggacgacggc cgcgatgagg ctgatgccga
     2941 tccagaagag gaagctcacg aggtcgatgg agctcaggac ggcgtccaag gacgccagct
     3001 ggacgcccgc gcgaccgagc tcgacaccgt agacggcgtg cacggcgagg aacacgagcg
     3061 ccgcatagat gctcgaggcc gcgacgtaca ggcgcagcga tcgggggaat cgacccactc
     3121 ttgtaacgcc tccgctccgc ttcgggggtt tgctcggcgc gtgcccgttc gggaacgccg
     3181 taggagcgcc cttcctcaca ccgaatcgta tccgtacgtg ggtacactga gagcacgacc
     3241 gacgcgagga gggacacggt gtccgcgacg aactacgtgc ccatggtcat cgagcagtcc
     3301 ggacgcggcg agcgcgccta cgacatcttc tcgcgcctgc tgaaggagcg gatcatcttc
     3361 atcggggacg ccatcgagga caccatggcg aacctgatca tcgctcagct tctcttcctc
     3421 gagtccgagg accccgagcg cgacatctac ctgtacatca actcgcccgg cggcgtcgtg
     3481 accgccggcc tcgcgatcta cgacaccatg cagtacctga aggcgcccgt ctccaccatc
     3541 tgcgtcggcc aggccgcctc gatgggtgct gtgctcctcg cggccggcac gaagggcaag
     3601 cgctacgcgc tgccgaactc gcggatcatg atccaccagg gttccggcgg cttccgcggc
     3661 aacacgccgg acgtgatgat ccaggtgaag gagctcgaga cgctcgtcga caagctcatg
     3721 aagatcatcg cgcaccacag cggccaggac tacgacaagg tcaagcgcga cgtcgaccgg
     3781 gaccggttca tgagcgccga ggaggcgaag gcctacggac tcatcgacga catcttcgtc
     3841 ggcaaggaat cgctcatctc gatcgcgaag gccgaggaga aagagcgcgt tccggcgggg
     3901 acccggtgac cacgaccgca aagggcggga agccataccg ctgctccttc tgcggcaaga
     3961 gccaggacca ggtgcgcaag ctcatcgcgg gccagggcgt gtacatctgc gacgagtgca
     4021 tcaccctctg tcgcgagatc gtggacgagg agttcatgga ggcgccgaag gcgcggacgc
     4081 tcggccagaa gatcccgagc ccccggcgca tcaacgagat cctcgatcag tacgtcatcg
     4141 gacaggacct cgcgaagcgc gtgctctcgg tcgccgtgta caaccactac aagcgcgtgt
     4201 cggcgggcca ccgcgtcgat gacgtcgagc tcggcaagtc gaacgtcctg ctcatcggac
     4261 cgaccggctg cgggaagacg ctcctcgcgc agaccctcgc gcgcatcctc gacgtcccgt
     4321 tctccatcgc cgacgcgacg agcctcaccg aggccgggta cgtcggcgag gacgtcgaga
     4381 acatcctcct ccgtctcatc caggccgccg acttcgacct gaaccgcgcg cagcggggga
     4441 tcatctacat cgacgagatc gacaagatcg cgcgcaaggg cgacaacccg tcgatcaccc
     4501 gcgacgtgtc gggcgaaggg gtgcagcagg cgctcctcaa gatccttgag ggcaccgtcg
     4561 cgaacgtgcc gccgcagggc ggccgcaagc atccgcacca ggacttcatc cagatcgaca
     4621 cgacgaacat cctcttcatc tgcggcggcg cgttcgaggg tctcgagaag atcgtcgagg
     4681 cgcgcgtcgg caagaagacg atgggcttcg gcgccacgct gcgcgacaac accaaggaga
     4741 cgagcgcggt cctgcagcag gtgatgcccg acgacctgct gaagtacggg ctcatccccg
     4801 agttcgtcgg tcgtctcccg gtgatcgtgc cgctcatggc gctcgacgag aacgacctca
     4861 tgcgcatcct caccgagccg aagaacgcga tcgtcaagca gtaccagaag ttcctggccc
     4921 tcgacaaggt cgagctgcag ctcaccgacg aagcactgcg ctcggccgcg gcgggcgccg
     4981 cgaagcgcaa gaccggcgcc cgcggcctgc ggacgatcat cgaggaggtc ctgctcgacg
     5041 tgatgtacga gatcccgtcg cgcaacgacg tgcggcgcgt cgtcgtcacg cccgaggcga
     5101 tcgacgggac ggccaagccg ctcctcctca gcaagaacga gcagctcgtg ctcgaagggg
     5161 acgaggcgac tgcctaagct ctactacgcg tccccgcgtg accttcccgc gttcgcggcg
     5221 tggcccggcg cgcagggtcg tgtcctccag accgacggcg ccacgatgat ccagttcgac
     5281 ctcgatccga aggtcgtcgt cgagacccac aagcacgacg tcgagcagtt cggcgtcgtc
     5341 gtcaagggct cgctcgcgat ggtgatcgcc ggcgagcagc gcatcctcac gccgggcgac
     5401 acgtaccgca tcccgccggg ggcagcccac ggggcccgcg tcttcgagga gcagacccgg
     5461 gtcatcgacg tctgggcgcc gccgcggacc gacctcctga agaagga
//
LOCUS       DASTZR010000339         3441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1011653, whole genome shotgun sequence.
ACCESSION   DASTZR010000339 DASTZR010000000
VERSION     DASTZR010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011653"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VFV20_07565"
     CDS             complement(<1..238)
                     /locus_tag="VFV20_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5288243.1"
                     /translation="MIVLDTTIVNVTLPSIRTDLGFSESSLAWVVNAYMLFFGGFLLL
                     GGRLGDLYGHRRLFVTGLALFTVASLAAGLASSQG"
     gene            complement(691..1674)
                     /locus_tag="VFV20_07570"
     CDS             complement(691..1674)
                     /locus_tag="VFV20_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018519138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5288244.1"
                     /translation="MQGTTARAPEGDLGRLLDQHRVELTGYCYRMLASPFEAEDAVQE
                     TFLRAWRAYETFEGRAAVRTWLHRIATNVCLDMLRSRKHRVRPMDLGPAREPIASNLN
                     VPSEVTWLEPIPDTLVATDPEPEETAVAKESVRLAFVAALQHLPARQRAALILCEVMS
                     WKAAEVAELLETSVASVNSSLQRARATLKTAGTSSFERAPVMDDAKRELLARYVEAFE
                     RYDVSLLTKVIREDAHQSMPPFDLWLSGRDDMFRWWFGPGIACNGSKLVPTHSANGSP
                     AFGQYKPDPNGGYAPWSLQVVELTPSGIDEITFFLETEKLFPLFGLPLHLD"
     gene            complement(1778..2449)
                     /locus_tag="VFV20_07575"
     CDS             complement(1778..2449)
                     /locus_tag="VFV20_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020524835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288245.1"
                     /translation="MKRRTLDTIFAGGGLLVAALLVVLGFIVAGQAAFATNYVKEELG
                     AQKITFATADKLTDAEKNWQPGSSCLVTYAGETMTTGAQAECYAKYYIREHMHASGVN
                     AGFPGATYATLGSIRSDLTAQVAAAKTAGNTTQAADLQKKLDAATSLRSTLQSGETLS
                     GLLLTVYGFSVIGNIANIAGNVLYALAGVMVLLSIAGFIHAWVTPKDKVVFAPPVGVG
                     ARVPA"
     gene            2700..>3441
                     /locus_tag="VFV20_07580"
     CDS             2700..>3441
                     /locus_tag="VFV20_07580"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5288246.1"
                     /translation="MDGEFRKLWAGQAVSVVGSNITLIALPLTAALTLAATPQEMGLL
                     TAAGWLPILLFSLLAGVWADRLQRRPVLIATDIARAAILGTIPVAALLGVLTIAHVLI
                     AAFLAGAMTVLFRSAYVPFIPALVPREGLVDANARLALNESVARVLGPSLGGVLVQVV
                     SAPVAIAADAVSFLVSAYAIASIRVTETAPDPAKRRSIWIEMGEGMRVVARNAFVRSV
                     TIIAGIFNLAIITGETIYILYATRVLGLD"
BASE COUNT          508 a         1152 c         1213 g          568 t
ORIGIN      
        1 tgccctgcga cgacgcgagc cccgccgcga gcgacgcgac ggtgaagagc gcgagcccgg
       61 tgacgaagag ccggcggtgc ccgtagaggt cgcccagccg tccgccgagg agcaggaagc
      121 cgccgaagaa gagcatgtac gcgttcacga cccaggcgag cgagctttcg ctgaagccga
      181 gatccgtgcg gatcgacggc agcgtcacgt tcacgatcgt cgtgtcgagc acgatcatga
      241 agctgccgaa gcagaggacc acgaaggcca gccagcgagc gcggttttca gtgttcacgt
      301 cgtccctccc ggtggtttcg ctgcttagac gcaccgggcc gcggaactca tcgcgctggg
      361 aagcgatgag ttcccgtcct cgcgctcgtc tattcgcttg gatgggcagc gagaggccgc
      421 gggcggcccg cctggccata cgggggcccc gggagcgtcc ggacctgccg ggcgtctacg
      481 cgcgcgcgtg cgccgtgctg gcggagaacc aaggggcggc catggactgc gacgtcgcgg
      541 gcatcgcggc ggacgccgtg gccgtcgagg cgctcgcgcg gctccagctc ggagcgctgc
      601 ggcacgggtg cggcgtgcgt cttgtgaacg cgtcgcgcga gctcgccgac ctggtcgcgt
      661 tcatgggcct cggggacgtg ctgcccctcg tcagtcgaga tggagcggca ggccgaagag
      721 ggggaagagc ttctcggtct ccaggaagaa cgtgatctcg tcgatgccgc tcggcgtgag
      781 ctcgacgacc tgcagcgacc acggtgcgta accgccgttc gggtccggct tgtactgccc
      841 gaatgccggc gaaccgttcg cactgtgcgt cggcaccagc ttcgagccgt tgcaggcgat
      901 cccgggcccg aaccaccacc ggaacatgtc gtcgcgtccg ctgagccaga ggtcgaacgg
      961 cggcatcgac tgatgcgcgt cctcgcggat gaccttcgtg aggaggctca cgtcgtagcg
     1021 ctcgaaggcc tcgacgtatc gcgcgaggag ctcgcgcttc gcgtcgtcca tcaccggagc
     1081 gcgctcgaac gacgacgtgc cggcggtctt gagggtcgcg cgggcgcgct ggagcgagct
     1141 gttcacgctc gcgacgctgg tctcgaggag ctcggcgacc tcggccgcct tccagctcat
     1201 gacctcgcag aggatgagcg ccgcgcgctg ccgtgcggga aggtgctgca gcgcggcgac
     1261 gaacgcgagc cgcacggact ccttcgcgac cgcggtctcc tcgggctcgg ggtcggtcgc
     1321 gacgagcgtg tcggggatcg gctcgagcca cgtgacctcg ctcgggacgt tgaggttcga
     1381 cgcgatcggc tcgcgcgccg gtccgaggtc catcgggcgc acgcgatgct tccgcgaccg
     1441 cagcatgtcc aggcagacgt tggtcgcgat gcgatgcagc cacgtcctga ccgccgcgcg
     1501 cccttcgaac gtttcgtacg cgcgccaggc gcgcaggaac gtctcctgca ccgcatcctc
     1561 cgcctcgaac ggcgaggcca gcatgcggta gcagtagccg gtgagctcga cccgatgctg
     1621 gtcgagcagg cgcccgaggt cgccttccgg cgccctcgcc gtcgtcccct gcatcggagg
     1681 aatggtaatg aaagaaaccc ggtctgtacg accgggtttc cttcggaagg aggccacttc
     1741 gcgatgtcgt cgcgggtggc tctgggtgag gggctggtta ggcggggacc ctggcgccca
     1801 caccgaccgg cggagcgaag acgaccttgt ccttgggcgt gacccaggcg tggatgaagc
     1861 cggcgatcga gagaaggacc atgacgcccg cgagggcgta caggacgttc ccagcgatgt
     1921 tggcgatgtt gccgatcacg ctgaagccgt acaccgtgag gagcagaccc gagagggtct
     1981 cacccgactg cagcgtcgag cgcagcgacg tcgcggcgtc gagcttcttc tggaggtcgg
     2041 cggcctgcgt ggtgttcccg gcggtcttcg ccgccgcgac ctgggccgtg aggtcactgc
     2101 ggatcgagcc gagcgtcgcg taggtggcgc ccgggaatcc ggcgttcacg cccgatgcgt
     2161 gcatgtgctc gcggatgtag tacttcgcgt agcactcggc ctgcgcgccg gtggtcatgg
     2221 tctcgccggc gtaggtcacg aggcaggacg aaccgggctg ccagttcttc tcggcgtcgg
     2281 tgagcttgtc cgcggtggcg aacgtgatct tctgcgcgcc gagctcttcc ttgacgtagt
     2341 tggtcgcgaa tgcggcctgg ccggccacga tgaaaccgag gaccacgagc agcgcggcga
     2401 ccaggaggcc gccgccggcg aagatggtgt cgagcgttct gcgcttcatt cgtttcttcc
     2461 tcccgccttg tctagggcga tcgagctttg cgcaagacat tcgacgatga gcgaaggacg
     2521 caacagcgcc gtatggctca gaccatcgtc actctcggca ctcacgttcg acgcgaacga
     2581 catcacttct cagcgcgctc tccgcgcgat cggtcacggc ctcagccgtt cctcaatggc
     2641 acctcagcca ttcctcaatg tgccgcgccg tcggccgggg gaagcgatac cgtccgccca
     2701 tggacggcga gttccggaag ctctgggcgg gccaggccgt atccgtcgtg gggtcgaaca
     2761 tcaccctcat cgcgctgccc ctcacggccg cgctgactct cgccgcgacg ccgcaggaaa
     2821 tgggactgct caccgcggcg ggctggctgc cgatcctgct gttctcgctc ctcgcaggcg
     2881 tgtgggcaga ccggcttcag cggaggccgg tgctgatcgc caccgacatc gcgcgtgccg
     2941 cgatcctcgg caccattccc gtcgccgcgc tgctcggcgt gctcacgatc gcgcacgtcc
     3001 tgatcgccgc cttcctcgcg ggcgcgatga ccgtgctctt ccgctcggcc tacgtcccgt
     3061 tcatcccggc gctcgtcccg cgtgagggat tggtcgacgc caacgcgcgg ctcgcgctga
     3121 acgaatcggt ggcgcgcgtg ctgggtccga gcctcggcgg ggtgctcgtg caggtcgtga
     3181 gcgccccggt cgccatcgcg gccgacgcgg tgtcgttcct ggtgtccgca tacgcgatcg
     3241 cgtcgatccg cgtgacggag acggcgcccg acccggcgaa gcggcgcagc atctggatcg
     3301 agatggggga gggcatgcgc gtggtcgcgc gcaacgcatt cgtgcgcagc gtcacgatca
     3361 tcgcgggcat cttcaatctc gcgatcatca ccggcgagac catctacatc ctgtacgcga
     3421 cgcgcgtgct cggcctggac g
//
LOCUS       DASTZR010000340         2092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_150092, whole genome shotgun sequence.
ACCESSION   DASTZR010000340 DASTZR010000000
VERSION     DASTZR010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150092"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VFV20_07585"
     CDS             <1..276
                     /locus_tag="VFV20_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288247.1"
                     /translation="HAVAEGARAGALTNDDEIIRETLAAAAAPLAAERITTAIQPPAS
                     DAPRNADPRGSLLTVRASYDLPLPLAFVAIPRLVVSASATRRIEWTP"
     gene            273..692
                     /locus_tag="VFV20_07590"
     CDS             273..692
                     /locus_tag="VFV20_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288248.1"
                     /translation="MSDDRGQAAILAVCLIAIAAAVMGGLRVAQTALLARAVERRAGE
                     AAVEAATAVVADAYAAEVRARRSPSGASRDVASVVGDAITREAARAGASDMSVRNGGE
                     SVSDVAVACDAGAVTVTIELRGTLYRASFEAPECSRR"
     gene            677..1093
                     /locus_tag="VFV20_07595"
     CDS             677..1093
                     /locus_tag="VFV20_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288249.1"
                     /translation="MLPALTASRRRVDPRRAGLLATSAVLAIAAIGAQVTMAPAPKAP
                     AALADELRESSWAIEIPAAWLAARAPSVRRGDALDILAVRSGDRAYAVPVAYAVIVMS
                     ADDRALVLQIDQDDAIAIANARGAGLMLIPLLRSTR"
     gene            1090..2031
                     /locus_tag="VFV20_07600"
     CDS             1090..2031
                     /locus_tag="VFV20_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288250.1"
                     /translation="MSEQIRVAVFGGDELRASITALGFVPVDEDARIAVIDTNDPQAL
                     VRAAALPPEVPRALLGPAAHRALVAATGLPARFADSAEPAVLGPLLLSLVPPRPRART
                     RIVVVTGVRGGVGRTLLATNLARRLARHTRAVFVDATGSGAAAWWLRCEAASWSTLEG
                     LVDEMSPDHLAVVAHDAGPQLRLVGGPFVAPSEAILLATVRAAATTDDIVVVDAPPAW
                     HRENGSLRSIADRWLVLSYDDGSSAAALRAAAPTDDDWVIASQSRAQRIGDVPTFRTL
                     PRDERAVATALDRRGAVGGALGRAYDDLAELVAIDAS"
BASE COUNT          241 a          794 c          762 g          295 t
ORIGIN      
        1 catgccgtcg cggaaggcgc ccgcgccggc gcgctcacga acgacgacga gatcatccgc
       61 gagacgctgg ccgcggcggc cgcgccgctc gcggcggagc ggatcacgac ggcgatccag
      121 ccgccggcat cggacgcacc ccgcaatgcc gacccgcgcg gctcgctgct cacggtgcgg
      181 gcgagctacg acctgccgct gcccctcgcc ttcgtcgcga tccctcgact cgtcgtcagc
      241 gcgtcggcca cgcgccgtat cgagtggacg ccgtgagcga cgatcgcgga caggcggcga
      301 tcctcgcggt ctgcctcatc gcgatcgccg cggccgtcat gggtggactt cgcgtcgcgc
      361 agacggcgct cctcgcgcga gcggtggagc gccgcgcagg cgaggcggcg gtcgaggccg
      421 cgacggccgt cgtcgccgat gcgtacgccg ccgaggtgcg cgctcggcgg agcccatcag
      481 gcgcctcgcg cgacgtcgcg tcggtggtcg gcgatgcgat cacgcgtgag gccgcgcgcg
      541 ctggggcatc cgacatgagc gtgcgcaacg gcggcgagag cgtgtccgac gtcgcggtcg
      601 cctgcgacgc cggcgcggtg acggtcacga tcgagctgcg cggcacgctc tatcgcgcga
      661 gcttcgaggc gccggaatgc tcccggcgct gaccgcgtcg cggcggcgcg tcgatccgcg
      721 ccgtgccggc ctcctggcca catcggccgt gctcgcgatc gcggcgattg gcgcgcaagt
      781 cacgatggca cccgcgccga aggccccggc cgcgctcgcc gacgagctgc gtgagtcgtc
      841 gtgggcgatc gagatcccgg ccgcatggct cgcggcgcgc gcaccatcgg tgcggcgagg
      901 cgacgcgctc gacatcctgg ccgtgcgctc gggcgatcgt gcctacgcgg tgcccgtcgc
      961 ctacgcggtc atcgtgatgt ccgccgacga ccgtgcgctc gtgctccaga tcgatcagga
     1021 cgatgccatc gccatcgcca acgcgcgggg cgccggactg atgctcattc cgttgctccg
     1081 ctcgacgcgg tgagcgagca gatccgcgtc gccgtcttcg gcggcgacga actccgcgcg
     1141 tcgatcaccg cgctcggctt cgtccccgtc gatgaggacg cgcggatcgc cgtcatcgac
     1201 acgaatgacc cgcaggcgct cgtgcgggcc gcggccctgc cgccggaagt accgcgcgcc
     1261 ctgttgggcc cggcggcgca tcgtgcgctc gtcgccgcca cgggtctgcc cgcgcgtttc
     1321 gccgactcgg cggagcccgc cgtccttggc ccgctgctcc tttcgctcgt cccgcccagg
     1381 ccccgtgcgc gcacgcgcat cgtcgtggtg acgggcgtcc gcggcggcgt cggccggacc
     1441 ctcctcgcca cgaacctcgc gcggcgtcta gcgagacaca cacgggccgt cttcgtcgat
     1501 gcgaccggga gcggcgccgc cgcgtggtgg ctgcgctgcg aggctgcgtc atggtcgacg
     1561 ctcgagggcc tcgtcgacga gatgtcgccc gatcacctcg cggtggtcgc gcacgacgcg
     1621 ggcccgcagc ttcgcctcgt cggcggtccg ttcgtcgcgc cgagcgaggc gatcctgctc
     1681 gccaccgttc gcgccgccgc gaccacggac gacatcgtcg tcgtggatgc gccaccggca
     1741 tggcatcgcg agaacggatc gctgcggtcc atcgccgacc ggtggctcgt gctgagctac
     1801 gacgatggca gctccgccgc ggccctgcgc gcggccgcgc cgaccgatga cgattgggtc
     1861 atcgcctcgc aatcgcgcgc gcagcggatc ggcgacgtgc cgacctttcg cacgctgccg
     1921 cgcgacgagc gtgcggtcgc gacggcgctc gaccgccgtg gcgccgtcgg cggcgcgctc
     1981 ggtcgcgcct atgacgatct cgctgagctc gtcgcgatcg acgcgtcgtg acatccgagg
     2041 ctcagcgcgc gcggctgcag caggccgtcg tcgcgcggct gcgcgacgtg cc
//
LOCUS       DASTZR010000341         2248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_620317, whole genome shotgun sequence.
ACCESSION   DASTZR010000341 DASTZR010000000
VERSION     DASTZR010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620317"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="VFV20_07605"
     CDS             complement(<1..943)
                     /locus_tag="VFV20_07605"
                     /inference="COORDINATES: protein motif:HMM:NF005914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5288251.1"
                     /translation="MASANALRERVHAAMPKVREDLERLVAIPSVSAKDWDGAPLRRS
                     AETVAEMLRGVGAEVKILTSEQGHPAVVGYFPAPAGAPTVLLYAHHDVQPPGPESEWG
                     SPPFTPTVRDGRLFGRGSSDDKAGVVAHIAALRAFGGKPPVGVAVFIEGEEEAGSENL
                     RSYLEQERARLACDAVVVADGGNWRLGEPALTTTLRGVVSCVVELRVLDKAVHSGQFG
                     GTFPDALTAMAHLLATLHDKKGDVAVQGIARGKAKKLDLTEEELRTQAGVRQGVQLIG
                     KGGLTDRLWMKPSVSVLAIDAPPVRDAINQLVPVARAK"
     gene            995..1297
                     /locus_tag="VFV20_07610"
     CDS             995..1297
                     /locus_tag="VFV20_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288252.1"
                     /translation="MRTDVALPTRREWTEDDDDLLGSILSLRETWRECRGRVTLARLA
                     LDPRLATFVLSGVCPRHGQFVREWTGRVCDALPLEASCSAADRGRCHEIAPVFVLV"
     gene            complement(1294..1809)
                     /locus_tag="VFV20_07615"
     CDS             complement(1294..1809)
                     /locus_tag="VFV20_07615"
                     /inference="COORDINATES: protein motif:HMM:NF045466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288253.1"
                     /translation="MPTLWHASEDAHIEIFRRHRARTAQVDEPLVWAIDDEHVPAYWF
                     PRDCPRATFWITERTSAEDRAWIHGAARVHAIEWDWWERFRDARVYLYLMPPDAFAVH
                     DADAGYYVAHEPVIPLATVEIDDLVRRHAGAGIELRVVSELWPLWDRVIRSTLGFSGI
                     RLRNARARTDR"
BASE COUNT          317 a          810 c          783 g          338 t
ORIGIN      
        1 ccttcgcccg cgcgacgggc acgagctggt tgatcgcgtc gcgcaccggc ggcgcgtcga
       61 tcgcgaggac cgacacgctc ggcttcatcc agagacgatc ggtgagcccg cccttcccga
      121 tgagctgcac gccctgccgg acgccggcct gcgtgcgcag ctcctcctcg gtgaggtcga
      181 gcttcttggc cttgccgcgc gcgatgccct ggaccgcgac gtcgcccttc ttgtcgtgga
      241 gcgtcgcgag caggtgggcc atggcggtga gcgcatccgg gaacgtgccg ccgaactgtc
      301 cggagtggac ggccttgtcg agcacccgca gctcgacgac gcacgagacc acgccgcgca
      361 gcgtcgtcgt cagcgcgggc tcgccgagcc gccagttgcc gccatcggcg acgaccacgg
      421 cgtcgcacgc gagcctcgcg cgctcctgct ccagatagga gcgcaggttc tcggagccgg
      481 cctcctcctc gccctcgatg aagaccgcga cgccgaccgg cggcttgccg ccaaaggcgc
      541 ggagcgccgc gatgtgcgcc acgaccccgg ccttgtcgtc cgagcttccg cgcccgaaga
      601 gccggccgtc acgcaccgtc ggggtgaagg gcggcgaacc ccactcgctc tccggcccgg
      661 gcggctggac gtcgtgatgc gcgtagagga gcaccgtcgg cgccccggcc ggcgccggaa
      721 agtacccgac gaccgcgggg tgcccctgct cgctggtaag gatcttcacc tcggcgccga
      781 cgccgcggag catctcggcc acggtctcgg ccgagcgccg aagcggcgcg ccgtcccagt
      841 ccttcgcgct gacggatggg atggcgacga ggcgctcgag gtcctcgcgg accttcggca
      901 tggcggcgtg gacccgttcc cggagggcgt tcgcactggc catgccggga agtatgcgtt
      961 tccggcccgg tacgcgctgt gcatagcccg cggcatgcgc acggacgtgg ccctgccgac
     1021 ccggcgggaa tggaccgagg acgacgacga cctcctcggc tccatcctct cgctccgaga
     1081 gacctggcgg gagtgccgcg ggcgggtcac cctcgcgcgc ctggcgctcg atccgcgcct
     1141 cgccaccttc gtgctctccg gcgtctgtcc gcgccacggc cagttcgtgc gcgagtggac
     1201 ggggcgggtc tgcgatgcgc tcccgctgga ggcgagctgc agcgccgccg atcgcggccg
     1261 ctgtcatgag atcgcgccgg tgttcgtcct ggtctagcgg tcggtgcgcg ccctcgcgtt
     1321 ccgcaggcgg ataccgctga agccgagcgt cgaacggatc actcggtccc agagcggcca
     1381 gagctcactc acgacgcgca gctcgatccc cgcaccggcg tgccgccgca cgaggtcgtc
     1441 gatctcgacc gtcgcgagcg gtatcaccgg ctcgtgcgcc acgtaatagc ccgcgtccgc
     1501 gtcgtggacc gcgaacgcgt cgggcggcat caggtagagg tacacgcggg cgtcgcggaa
     1561 tcgttcccac cagtcccact cgatggcgtg cacgcgcgcc gcgccgtgta tccacgcgcg
     1621 atcctccgcg ctcgtgcgct cggtgatcca gaacgtcgcg cgcggacagt cgcgcgggaa
     1681 ccagtacgcc ggcacgtgct cgtcgtcgat cgcccacacc agcggctcgt cgacctgcgc
     1741 cgtccgcgcg cgatgtcgac ggaagatctc gatgtgcgcg tcttcgctcg catgccacag
     1801 cgtcggcacc ggcgaatgtt agggagtggt cgcacgcgtc gaaaagcgcg cgcgggacgg
     1861 gggatcgccg cgtgcgggag agacgacgga gcctttcgaa cgagcatccg gccaaatagg
     1921 gcggcaagcg agcgcggcgc ccatggacac aattgcctct ttgcgccgtg tcacactcgc
     1981 cgggcctttg aaaggggaat accggatgag acggcgaatg atcctggctt tcctgatgac
     2041 ctgcgcgctc gtgatcgtga gcgctccaac gagctcctac gcggcggcgc ccaccaacgt
     2101 tgtgatcgcg ggcagcgtga ccggagcact cccggcgccc tgcaattgcc ccgtcaccgt
     2161 gtacgtgagc atggtggcat ctggagtccc cacggccttg agcgggtccg gggtggcaca
     2221 tgcatcgacg ggcgccacga atcgtttc
//
LOCUS       DASTZR010000342         2258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_907369, whole genome shotgun sequence.
ACCESSION   DASTZR010000342 DASTZR010000000
VERSION     DASTZR010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907369"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="VFV20_07620"
     CDS             <1..1098
                     /locus_tag="VFV20_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288254.1"
                     /translation="LVFAYAVGGFLFGRGAGDVTGGPTATASGTAVPKPPEVTFAQET
                     CCTQTARYMKVAWTASEKVTAAKVTVIPDPGFPCESAIDASGLKGVMTCQGLLKGATD
                     YAAALSLVTARGTYPYQHKFKTMGDRLANVQWFTEFEDPRGDPLACAAASVRIVQNYT
                     TGKDAATATQILQQGQAYNRSKDTGIDPVAIATMQKKLDARNNYHYYRFPTREEATKS
                     AVYWLVRSGKPVHVISLAGQHDPLLVGFTGAFGTYYDDPVNSFTQIVVMDPQRGDTNP
                     QTANHRPDKSRSVGFQTGQALELNEWYGDEWWLRFVYATSARQPDGSFINIERNDGAY
                     PTPHWGGQFVILVDDGDADWPSDKEGRVKWH"
     gene            complement(1117..1509)
                     /locus_tag="VFV20_07625"
     CDS             complement(1117..1509)
                     /locus_tag="VFV20_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEU5288255.1"
                     /translation="MTSASQVIDKRIRDLAGWRGETLARMRALILGADPEMTEEIKWI
                     KASNPGTPVWSHHGIVCTGEVYAKVVKLTFARGASIPDPAHLFNSSLDGNTRRAIDIH
                     EGDTVDARAFRALVKAAVAANARSATKR"
     gene            complement(1564..2037)
                     /locus_tag="VFV20_07630"
     CDS             complement(1564..2037)
                     /locus_tag="VFV20_07630"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU5288256.1"
                     /translation="MTVTIGPIWQGPLYRRLCVALLVGIVGFGLTLLTIGARSPYTHS
                     NLGRGFDPGYTRTPQALVGEQAEFAGLGTDGAPAGADAIARGRFLFVTQGCAGCHALE
                     AQGGPVGPAIAGTDAETVTQRVRQGPVGMPQFSAASLTEAEISAITAYLASLASK"
     gene            complement(2034..>2258)
                     /locus_tag="VFV20_07635"
     CDS             complement(2034..>2258)
                     /locus_tag="VFV20_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288257.1"
                     /translation="GVRVPGLNEAPRAGVVLGEANAVAAMGAQLWPALSGWPIAAVLV
                     IVWSLYAFVALLVIRVARVEETAPRRKHVA"
BASE COUNT          369 a          781 c          794 g          314 t
ORIGIN      
        1 ctcgtcttcg cgtatgccgt cgggggcttc ctcttcggac gcggtgcggg tgacgtcacg
       61 ggcgggccga ccgcgaccgc gagcggtacg gcggtgccga agcctcccga ggtcaccttc
      121 gcgcaggaga cctgctgcac gcagacggct cgctacatga aggtcgcgtg gaccgcgagc
      181 gagaaggtga ccgccgcgaa agtcacggtc atccccgatc cgggattccc gtgcgagtcg
      241 gccatcgacg cgagcgggct gaagggcgtg atgacctgcc aggggctgct gaagggcgcc
      301 accgactatg ccgccgcgct ctcgctcgtc acagcccgcg ggacctaccc ctaccagcac
      361 aagttcaaaa cgatgggtga ccgtctcgcg aacgtgcaat ggttcaccga gttcgaggat
      421 cccaggggcg atccgctggc ctgcgccgcg gcgagcgtgc gcatcgtgca gaactacacc
      481 acggggaagg atgcggccac ggcgacgcag atcctccagc agggacaggc gtacaaccgg
      541 agcaaggaca ccggcatcga ccccgtcgcg atcgcgacga tgcagaagaa gctcgacgcg
      601 cgcaacaact atcactacta ccgcttcccg acccgtgagg aggcgacgaa gtccgcggtc
      661 tactggctcg tgcgcagcgg caagccggtg cacgtcatca gcctcgccgg gcagcacgat
      721 ccgctgctcg tcggcttcac cggcgcgttc gggacgtact acgacgatcc cgtcaactcg
      781 ttcacgcaga tcgtggtcat ggacccgcag cgcggcgaca cgaacccgca gaccgcgaac
      841 caccgtcccg acaagtcgcg gagcgtgggc ttccagaccg gtcaggccct cgagctgaac
      901 gagtggtacg gcgacgagtg gtggctgcgg ttcgtctacg ccacgagcgc gcgccagccg
      961 gatgggtcat tcatcaacat cgagcggaac gacggcgcgt acccgacgcc gcactggggc
     1021 ggacagttcg tgatcctggt ggacgacggc gacgccgact ggccctcgga caaagagggc
     1081 cgggtgaagt ggcactagcg gggagcgtct aggcgttcac cgcttcgtcg ccgaccgggc
     1141 attcgcggcg accgcggcct tcacgagcgc cctgaacgcg cgcgcgtcga ccgtgtcgcc
     1201 ctcgtggatg tcgatcgccc ttcgcgtgtt gccgtccagg ctggagttga agaggtgcgc
     1261 tgggtcgggg atgctcgccc cccgcgcgaa ggtgagcttc acgaccttcg cgtacacctc
     1321 accggtgcag acgatcccgt ggtgcgacca gaccggagtg cccgggttcg acgccttgat
     1381 ccacttgatc tcctcggtca tctcgggatc ggctcccagg atgagcgccc gcatgcgggc
     1441 gagggtctcg ccgcgccacc ccgcgaggtc gcggatccgc ttgtcgatga cctgggaggc
     1501 ggacgtcacg tgtagcgcga cgtgagccgc gaagccgtgg agcccatgcg agcagggtag
     1561 cggctacttc gaggcgagcg acgcgagata cgcggtgatc gccgagatct ccgcctcggt
     1621 gaggctcgcc gccgagaact gcggcatgcc gaccggtccc tgacgcacgc gctgcgtgac
     1681 ggtctcggcg tccgtgccgg cgatcgccgg gccgacgggc ccgccctgcg cctcgagcgc
     1741 gtggcagccc gcgcatccct gcgtgacgaa caggaagcga ccgcgcgcga tcgcgtcggc
     1801 gcccgcgggc gcgccgtcgg tgccgaggcc tgcgaactcg gcctgctcgc cgacgagcgc
     1861 ctgcggcgtg cgcgtgtagc cggggtcgaa tccgcgtccg agattgctgt gcgtgtaggg
     1921 gctgcgcgcc ccgatcgtga ggagggtgag gccgaagccg acgatcccga caaggagcgc
     1981 gacgcacagg cgccggtaca ggggcccctg ccagatcggt ccgatcgtga ccgtcatgcg
     2041 acgtgcttcc ggcgcggcgc cgtctcctcg acgcgcgcga cgcggatcac gaggagcgcg
     2101 acgaacgcgt agagcgacca aacgatcacc agtaccgcgg cgatgggcca tcccgagagc
     2161 gcgggccaca gctgcgcgcc catggccgcg acggcattcg cctcgcccag gacgacgccc
     2221 gcccgcggcg cctcgttcag gcccgggacg cgcacgcc
//
LOCUS       DASTZR010000343         2254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_176149, whole genome shotgun sequence.
ACCESSION   DASTZR010000343 DASTZR010000000
VERSION     DASTZR010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176149"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="VFV20_07640"
     CDS             <1..993
                     /locus_tag="VFV20_07640"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5288258.1"
                     /translation="TQTCLALVSVALAVSTATGVVSVPLLYALTAIGAGFGALDQPAR
                     SALAPSLVERRQIPAAMALNQMVWRIGSVVGPAAGGVIIAAFGLAFAYWVDVGTFLVA
                     ILALALMRLPRHIPALEHPHVLRSLQEGWSFLLARPLIFAPMGLDFAVNLFGTTRALM
                     PYFADQVFHVGPQGLGLLYAAPGIGATVLLLTSGWTSAVQRKGLGILIAIGVYGVANL
                     AFAFVPSSMFLLACALLALAEGGDSASVIFRHTVLQMETPDALRGRLSSINSLFVAGG
                     PQLGQVESGFVADVLTPEISVAIGGMACVGLVVATAAAVPQLSRYRTDASVTPA"
     gene            1012..1989
                     /locus_tag="VFV20_07645"
     CDS             1012..1989
                     /locus_tag="VFV20_07645"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5288259.1"
                     /translation="MITLVREGDVTTSPVVMDRVTRRFRDVVAVNDASLEVAPGTILG
                     LIGPSGSGKTTMVRMLTGTLAQTSGDLWVLGERPSRFRRETRERIGYMPQQFHLYPHL
                     TTGENVAFCATLFGLFWWRRRRRVRQVLELTQLYDARDRLAMDLSGGMQRRLSLACAL
                     VHEPDVLFVDEPTAGLDPLLRQAVWEEFRRLRDTGHTLFVTTQSVSDAEYCDHVALLV
                     RGTIVALGEPEELRRAAFGGEVIEVRTRGAAPAGVVEKLPGVIDVQQPAKDRLVVIAE
                     DAGATTPRIADALNAWGSPVLTADKYEPTFDDVFVELVERSTEEPEAAS"
     gene            1986..>2254
                     /locus_tag="VFV20_07650"
     CDS             1986..>2254
                     /locus_tag="VFV20_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288260.1"
                     /translation="MIGIAKSLVRMSGLITKELRDLARRPGAILSLIFGPLAIMAIFG
                     LGYDGQRQPFDTIVILPQGSQLPHDVAYYQQMKTPGLRVIAVSEDR"
BASE COUNT          282 a          823 c          782 g          367 t
ORIGIN      
        1 acgcagacct gcctcgcgct cgtgtccgtc gcgctcgcgg tctcgacggc gaccggcgtc
       61 gtgtcggtgc cgctgctcta cgcgctcacc gcgatcggcg cggggttcgg cgcgctcgac
      121 cagccggccc gctccgcgct cgcgccgtcg ctcgtcgagc ggcggcagat cccggcggcg
      181 atggcgctca atcagatggt ctggcgcatc ggcagcgtcg tcgggccggc cgcgggcggc
      241 gtgatcatcg cggcgttcgg cctcgcgttc gcgtactggg tggacgtcgg cacgttcctc
      301 gtcgcgatcc tcgcgctcgc gctcatgcgg ctgccgcgcc acatcccggc gctggagcat
      361 ccgcacgtgc tgcgctcgct ccaggagggc tggtcgttcc tgctggcgcg cccgctgatc
      421 ttcgcgccga tgggcctcga cttcgcggtc aatctcttcg gcacgacgcg agcgctcatg
      481 ccgtacttcg ccgaccaggt cttccacgtg gggccgcagg gtctcggcct gctctacgcc
      541 gcgccgggca tcggcgccac ggtcctgctc ctcacgagcg gatggacctc cgccgtgcag
      601 cgcaagggcc tcggcatcct catcgccatc ggcgtgtacg gcgtcgcaaa tctcgcgttc
      661 gcgttcgtgc cttcgtcgat gttccttctc gcatgcgccc ttctcgcgct tgcggagggc
      721 ggcgactcgg cgagcgtcat cttccgccac accgtgctgc agatggagac acccgacgcg
      781 cttcgcggac gcctctcgtc gatcaacagc ctcttcgtcg ccggcggccc ccagctcggg
      841 caggtggagt cgggcttcgt cgccgacgtg ctcacgccgg agatctccgt cgccatcggc
      901 ggcatggcct gcgtcggtct ggtcgtcgcg acggccgccg cggtcccgca gctctcgcgc
      961 taccgcaccg acgcgtccgt tacaccggcg tagcacggca cgtaccttgc cttgatcacc
     1021 ctcgtgcgcg agggcgatgt aacgacgagc ccggtcgtca tggaccgggt cacgcggcgg
     1081 ttccgcgacg tggtcgcggt gaacgacgcg tcgctcgagg tcgcccccgg cacgatcctc
     1141 ggtctcatcg gcccgagcgg ctcgggcaag acgacgatgg tgcgcatgct caccgggacg
     1201 ctggcgcaga ccagcggcga cctctgggtc ctcggcgagc ggccgtcacg cttccgccgc
     1261 gagacgcgcg agcgcatcgg ctacatgccg cagcagttcc acctgtaccc gcatctcacc
     1321 accggcgaga acgtcgcgtt ctgcgccacg ctcttcgggc tcttctggtg gaggcgccgc
     1381 cggcgcgtgc gtcaggtgct cgagctcacg cagctgtacg acgcgcgcga ccgcctcgcg
     1441 atggatctct ccggcggcat gcagcggcgt ctctcgctcg cctgcgcgct ggtccacgag
     1501 ccggacgtgc tgttcgtgga cgagccgacc gcgggcctcg acccgctgct gcgccaggcg
     1561 gtatgggagg agttccggcg gctgcgcgac accggccaca cgctcttcgt gacgacgcag
     1621 tccgtgagcg acgccgagta ctgcgaccac gtcgcgctcc tcgtccgcgg cacgatcgtg
     1681 gcgctcggcg agccggagga gctgcgccgc gcggcgttcg gcggcgaggt catcgaggtg
     1741 cgcacccgcg gcgcggctcc ggcgggcgtc gtcgagaagc tgccgggggt catcgacgtg
     1801 cagcagccgg cgaaggatcg cctcgtcgtg atcgccgagg acgcgggagc gaccacgccg
     1861 cgcatcgccg acgcgctgaa cgcgtggggc tcgccggtgc tcaccgcgga caagtacgag
     1921 ccgacattcg acgacgtgtt cgtcgagctc gtcgagcgca gcaccgagga gccggaggcg
     1981 gcgtcgtgat cggcatcgcc aagagcctgg tgcgcatgag cggcctcatc acgaaggagc
     2041 tgcgcgacct cgcgcggcgc cccggcgcga tcctgagcct catcttcggt ccgctcgcga
     2101 tcatggcgat cttcggcctc ggctacgacg ggcagcgtca gccgttcgac acgatcgtca
     2161 tcctcccgca gggaagccag ctgccgcatg acgtcgcgta ctaccagcag atgaagacgc
     2221 ccggcctgcg ggtgatcgcg gtgagcgagg atcg
//
LOCUS       DASTZR010000344        11578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_254672, whole genome shotgun sequence.
ACCESSION   DASTZR010000344 DASTZR010000000
VERSION     DASTZR010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 11578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11578
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254672"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VFV20_07655"
     CDS             <1..330
                     /locus_tag="VFV20_07655"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU5288261.1"
                     /translation="LGAATMGLGAALREELIYEGDQLVNSNVVDYRVPRFSDQPREID
                     LILAERRDGAGPFGAKGAGEGAGNAMGSAVASAIARAIGVWPHQLPATPERVWRLMRD
                     RDERARR"
     gene            complement(342..710)
                     /locus_tag="VFV20_07660"
     CDS             complement(342..710)
                     /locus_tag="VFV20_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288262.1"
                     /translation="MENLPRPALAAAVAVVGIALVAIVVMSMAPATVEAQLQGYHLGN
                     DDRQLIVTAVMTRADSLKSVSAKQDATSVRVTVLLSRQKGGTYPLDLLFVPVTLMLER
                     ALGDRAVLDGKGDPIPLRRP"
     gene            complement(726..1742)
                     /locus_tag="VFV20_07665"
     CDS             complement(726..1742)
                     /locus_tag="VFV20_07665"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5288263.1"
                     /translation="MTDFYDDWLRRSAALDEAMRRTPVVARADALRWVTTVQDARTAM
                     IIGEAAGFQTSGTTLLIAEIPAGERSGRHAHGEEAIHILAGTGFSVIDGARYDWKPGT
                     TLHIPYRAEHQHVNMGTTTAVYLSAHTQDLDFAVKLGRLIQLEEKGAGDGGLAARYPA
                     QTSQFAADGRRIALHLEDAMDENARRLAGHAHPNAKADKHAGRHAGIWILMGGSESPT
                     DETNGFRASAVAMSTIFEEAPHSASHSHTHTEAMLYVLEGRGYSLIDGERHEWKAGDA
                     VHVPPRMTLHGHFNDSDERTRTLRIEFGIRYFYESLWSGYEKVKDKPSGHHGDEARQE
                     HHGH"
     gene            1851..2477
                     /locus_tag="VFV20_07670"
     CDS             1851..2477
                     /locus_tag="VFV20_07670"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU5288264.1"
                     /translation="MQSVEELVTTALRDAILSGELRPGDRLLQEQLAEQLRVSRIPLR
                     DALHRLEAEGLVRMGPRRGAEVASLTPADVREIYAIRIALEPELVRDAIAALTPVEID
                     RLIDMSEHMDDRIEDPAAGGRARRAFYSELYRFAGRPRTHRLIFALRDDVQRYHVMHD
                     QSEARHSHAEFREAIRAKDAERGARLLADHLRHACADLVQVLEGEATA"
     gene            2474..3436
                     /locus_tag="VFV20_07675"
     CDS             2474..3436
                     /locus_tag="VFV20_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5288265.1"
                     /translation="MKTEEVAFFSGGVKLRGYLHRPDAAAGRLPFIAQGPGWLGLADA
                     KLYRPYHEAFTAAGFAVLIFDYRGFGASEGDRGSLSPAWQLEDWRSAIAYLRGRDDID
                     ASRGAIFGSGGTGGGNAVLVAAYEPEMRATISQVPVADGRDWLHRMRAEHEWIAFLAS
                     VEADRRSRAATGEGTSVHPREGIMVATPERRATNVKADVDARIPSAVPLAAAQGILEY
                     RPLDVAPRVRGLMVVAVADDAVTPTDHAVALYEAAASPKQLVMQRNTSHYTAYTQYAS
                     EVIPMMVEWLRTLVLRGDDIEVRADRTAHASVRYIGGSEEGAKA"
     gene            3433..4827
                     /locus_tag="VFV20_07680"
     CDS             3433..4827
                     /locus_tag="VFV20_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HEU5288266.1"
                     /translation="MSSDVVIRGASVVLEDGIRKADIAIESGRISAIGEPGSVATGTR
                     EVDATDLTALPGMIDMHSHHREPGFTHKEDLVTMGKQCAAGGVTTSVAMPNVSPPPTT
                     AENLDAMLAVYRKRASFDYNVNASGVQLDEIPKLAERGILAFKIFMVVDTGRSYPHMP
                     GIGLHEHGELYKRMQAVKKTGLPLMVHPHDQSLMDAIEHEYWARGERDFRAYARAYAH
                     DDGVIWDTAVGVLLRLQAATGVRLHLLHTQTRRVVEMLRAAKAAGQTVSAEVNPWCFW
                     LDNDWANIERLGSYALSYFVQPHHAAAVYAAFLDGTVDIMATDHAPHLREEKEPGWTD
                     GWKAHTGTPSEQFYLSLLLTDAAAGKITLQRIAEATATRPAKLFGLYPQKGAIRVGAD
                     ADIVLVDAKAKRTIRDEDVLSKCGWTPYAGREVQGVPVYTFLRGEAIYDDGKIAIREG
                     FGRQARRAGAAVAR"
     gene            4862..5725
                     /locus_tag="VFV20_07685"
     CDS             4862..5725
                     /locus_tag="VFV20_07685"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5288267.1"
                     /translation="MTQVSVADSAAFRTIGPRRRQRRNVLHRWYRRNERLVLSLFGVV
                     AFFTFWQVGADAGIVDKFFFSSPRDIVVAGADEVQKARFWEDVWISSFELAVGTVLAL
                     IASVPLGLAIGWYRRLNYAFDPWLNFLNALPRVALIPLVVLTAGLGVEMKITVVFLGA
                     VFTIMVATVEGVRTVERQLLDVARSFRAPQRQIFTSLVIPGTLPFIVSGMRIAVGRVL
                     AGVIIAEFYAQTQGVGVMILKASAALNSPRMMFGILLFTLLGIVLYEAVGYAERYFQR
                     WRPSLDLEETT"
     gene            5722..6519
                     /locus_tag="VFV20_07690"
     CDS             5722..6519
                     /locus_tag="VFV20_07690"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5288268.1"
                     /translation="MTDQHLALTASGVQMEYRDRQTGKRLLALENVDLSVGDGAFVSL
                     LGPSGCGKSTFLKIVNGLLRQTSGEIKLHRVAKGREDAMVFQDASLFPWYTVTDNVAY
                     GLVCAGVPRKVARQRAQPYIDLVGLKGFGDKYPYQLSGGMQQRANLARALAVDSAILL
                     MDEPFASLDAQTRELMQAELLRIWNASKKTVLFVTHQIDEAVFLSDRVVVMAPRPGRV
                     IADITIDIPRPRSLDVKRTPRFQEYEQKIWQLIAGHVEETAVGATEP"
     gene            6519..7343
                     /locus_tag="VFV20_07695"
     CDS             6519..7343
                     /locus_tag="VFV20_07695"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5288269.1"
                     /translation="MASYPARLPSAMTSPLARLRGALGERVFYGLIGFVGVLILWETT
                     AQLGLYRKSLLSTPSAIWRVGLADTTSGMIWQHISISMTEFVIGFVVSLAIGIPLGLV
                     IGLFPRVDYLLNGLLAGLNATPNVALIPLIVLLAGIGMESKIVVVFLSAFFAVVVTTF
                     TGVQATARRHLEITRSFGGSQWLAFRSVVLPSTLPFIISGIRIGAGRALVGVVSAEFL
                     SANQGLGFYIALYGQFLDTARVMFGILIFGTLGLAIGEGVRFIERRFEAWRPELHH"
     gene            complement(7353..8114)
                     /locus_tag="VFV20_07700"
     CDS             complement(7353..8114)
                     /locus_tag="VFV20_07700"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5288270.1"
                     /translation="MRLADKAIVVTGAAHGIGRAYSERLASEGAQVALLDIDAKRVEE
                     VAEGIRASGGNALAIATDVRDFAQFKSAVDQAAKTFGKLTGIVNNAGMLNVIPITRCN
                     FEDIPDAEWDESFRLNSKSAWYGCKAAVPHLRANKGGSIVNIGSSTIFRGVPTRAHYV
                     AAKSSLIGFNRTIARELGKDYIRVNLVCPGSTLSEENPSPETVKMREDVIKTRCLPRL
                     ERPVDLVGTIAFLQSDDSAFTTGQTFLVDGGSAFH"
     gene            complement(8098..8901)
                     /locus_tag="VFV20_07705"
     CDS             complement(8098..8901)
                     /locus_tag="VFV20_07705"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU5288271.1"
                     /translation="MGGLNGGDTDVKARLDRIIAESAARKLTAQDLVLTSMREAILTG
                     TLAPGTRLRQEKLAEMFGTSRIPVREALRALEYEGLVTSLPYRGFTVTELDADDIEEV
                     YDLRVLLESHAVRLAVPLMTDEDLQTLEDLYAEMTAAEPGDAQLAARERFYIRLYSMT
                     GRHRLVALISRLRQEVARSLRWPTLQHAPEHHEQFFEAVRAGDVERAASQLASHYRRV
                     AILIRRYLRDASAMEKNGRRAPDARVNGETRSPTASTRTRRNADHASGG"
     gene            9030..10271
                     /locus_tag="VFV20_07710"
     CDS             9030..10271
                     /locus_tag="VFV20_07710"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5288272.1"
                     /translation="MRSRVGPFAQRAYTVYWGGGLVSNIGTWLQNVVAAVFVYDRTGS
                     ALAVGLLSFASWLPMLLFSVHGGMLSDRFDRRIIAIVTQTISLVVSAVLAVLAITGNA
                     NEYHVAITAFLLQTSWTIAKPSVTAMIPALVEREQLTEAVGLNTLQFQIAQLGGPVLA
                     TVLLTTSGYAWAFAINAVSFVAPVLAMAYLYVRGIGGPGSRAARKARADATGIVDYVR
                     GQQWIAWVMVGVISTSAVAEMIRTLSPVIVTHLGAPSSDTGLFVAAQSVGMVLGIFTS
                     VPLTRRGLARSLAPVGLVFQAVGLVVVATAPSMLVALIGGGLVGGGFSWCFPVLTGVL
                     QTEVPDAVRGRLLALHQMAHLGNRPFAALAAGAIAAAFGAPVACLAGVLLAPVGLVAV
                     RSAWRRLDHAAVPEPVIAESA"
     gene            10268..10474
                     /locus_tag="VFV20_07715"
     CDS             10268..10474
                     /locus_tag="VFV20_07715"
                     /inference="COORDINATES: protein motif:HMM:NF024851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEU5288273.1"
                     /translation="MSVRVSVDPDLCIGNAECARIAPAAFRLDDARGVSVVLPGASSV
                     DREVLEEAAFNCPTRAITVTDAPE"
     gene            10471..>11578
                     /locus_tag="VFV20_07720"
     CDS             10471..>11578
                     /locus_tag="VFV20_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEU5288274.1"
                     /translation="MSDEGLLTRYTATITFDRYEDIRAALFDPHLSRTFDKRSYEGGN
                     IRDGIVSIAHGATHRARRRIENTRFRAEVLRLYERELFPGVMNELLDTLITSDRVDLF
                     PIGEMLSVVLAARRAGIEYDPSSLDDLRTLVRHVDAFSQGSAILDAKDPDAVRALVRA
                     AYEDFERDFVRPAWERRAELIDAVRRGEAREQDLPQDILTVLLLHRADPSLELGDDGR
                     VVREVATYLQGGTHTSAQTLVNSLDLMFADPDPARLLERVARDTLFAQRVVQETLRLR
                     PTTPKAKRRAEADTEVAGRRIPKDALVVLDLASGNRDPEVWGATADQFDPDRTVPANV
                     PRWGLSFGAGAHQCPGRAVGGGFPIPASFDVDDDH"
BASE COUNT         1627 a         4099 c         4007 g         1845 t
ORIGIN      
        1 ctcggcgccg cgacgatggg cctcggcgcc gcgctgcgcg aggagctcat ctacgagggc
       61 gaccagctcg tgaactcgaa cgtcgtcgac taccgcgtac cgcgcttcag cgatcagccg
      121 cgcgagatcg acctgatcct cgccgagcgc cgcgacggcg ccgggccgtt cggcgcgaag
      181 ggtgccggcg agggcgccgg caacgcgatg ggcagcgcgg tcgcatccgc catcgcacgt
      241 gcgatcggcg tgtggccgca ccagctaccg gcgacgcccg agcgcgtctg gcgcctcatg
      301 cgcgaccggg acgagcgcgc tcggcggtag acagcgcgct gctacggtct ccggagaggg
      361 atgggatccc ccttcccgtc cagcacagcg cggtccccga gcgctcgctc gagcatcagc
      421 gtcacgggga cgaacagcag atccagcggg tacgtaccgc ccttttgtct cgacaagagc
      481 accgtgacgc gcaccgacgt cgcgtcctgt ttcgcgctca cgctcttcag tgagtccgcg
      541 cgtgtcatga cggccgtcac gatgagctgt cggtcgtcat tgccgaggtg atagccctgg
      601 agctgagctt cgaccgtggc cggtgccatg ctcatgacga cgatggcgac caacgcgatg
      661 ccgaccacgg cgaccgcagc ggcaagggcc ggccgcggaa ggttttccat gcgctgaccc
      721 ataggctagt ggccgtggtg ctcctgacgc gcctcgtcgc cgtgatgccc tgagggcttg
      781 tctttgacct tctcatagcc gctccagagc gactcgtaga agtagcggat cccgaactcg
      841 atgcgcagcg tgcgcgtgcg ctcgtcggag tcgttgaagt ggccgtgcag ggtcatccgc
      901 ggcggaacgt gaacggcatc gccggccttc cactcgtgac gttcgccgtc gatgagcgaa
      961 tagccgcggc cttcgaggac gtagagcatc gcctcggtgt gggtgtggct gtggctcgcg
     1021 gagtgcggcg cctcctcgaa gatcgtcgac atcgccacgg ccgaggcgcg gaagccgttc
     1081 gtctcgtcgg tgggactctc gctgccgccc atgaggatcc agatgcccgc gtgacggccg
     1141 gcgtgcttgt cggccttcgc gttcggatgc gcgtgtcccg cgagccggcg cgcgttctcg
     1201 tccatcgcgt cctcgagatg cagcgcgatg cgccgcccgt cggccgcgaa ctgcgacgtc
     1261 tgcgccgggt accgagccgc gaggccgccg tcgccggcac ccttctcctc gagctggatg
     1321 aggcggccga gcttcacggc gaagtcgagg tcctgcgtat gcgccgagag gtacacggcg
     1381 gtggtcgttc ccatgttcac gtgctggtgc tccgcccggt aggggatgtg gagcgtggtc
     1441 cccggcttcc agtcgtagcg cgcgccgtcg atgacgctga agcccgtgcc cgcgaggatg
     1501 tggatcgcct cctcgccgtg cgcgtggcgg cccgagcgct cacccgccgg gatctcggcg
     1561 atgagaaggg tcgtgccgga ggtctggaag cccgcggcct cgccgatgat catcgccgtt
     1621 cgagcgtctt ggaccgtcgt gacccagcgg agcgcgtcgg cgcgcgcgac gaccggcgtg
     1681 cggcgcatcg cctcgtcgag cgcggcgctg cggcggagcc agtcgtcgta gaagtcggtc
     1741 atctcgttct caatcctctc ggccccggtg ccctttcgtc ttgaccctcg ctatgtatac
     1801 gggatacgtt accggtcttg tccgtcccct cacgactcga ccgcccggac gtgcagagcg
     1861 tcgaagagct cgtcacgacg gcgctgcgcg acgccatcct ctcaggcgag ctccgtcccg
     1921 gcgatcgcct cctgcaggag cagctcgcgg agcagctccg cgtcagccgc atcccgctcc
     1981 gcgacgcgct gcaccgcctc gaggccgagg gcctcgtgcg catgggaccg cgccgcggcg
     2041 ccgaggtcgc gtcgctcacc cccgccgacg tccgcgagat ctacgcgatc cgcatcgctc
     2101 tcgagcccga gctcgtgcgc gacgcgatcg ccgcgctcac gccggtcgag atcgaccggc
     2161 tcatcgacat gtccgagcac atggacgacc gcatcgagga tcccgccgcg ggcggccgcg
     2221 cgcgtcgtgc gttctacagc gagctgtacc gtttcgccgg acggccgcgg acacaccgtc
     2281 tgatcttcgc gctgcgcgac gacgtgcagc gctaccacgt catgcacgac cagagcgagg
     2341 cgcgccattc gcacgccgag ttccgcgagg ccatccgcgc caaggacgcc gagcgcggag
     2401 cgcgcctcct cgcggaccac ctgcggcacg cgtgcgccga tctcgtgcag gtgctcgagg
     2461 gggaggcgac ggcgtgaaga ccgaagaggt cgcgttcttc agcggcggcg tgaagctgcg
     2521 cggctacctg caccggccgg acgcggccgc cggacgcctg ccgttcatcg cgcaggggcc
     2581 cggctggctc gggctcgccg acgcgaagct gtatcgcccg taccacgagg cgttcaccgc
     2641 cgccggattc gcggtgctca tcttcgacta tcgcggcttc ggcgcgagcg agggcgaccg
     2701 cggctcgctg tcgcccgcgt ggcagctcga ggactggcgc agcgcgatcg cgtacctgcg
     2761 cggccgggac gacatcgacg cgtcgcgtgg cgcgatcttc gggagcggtg gcaccggtgg
     2821 aggcaacgcc gtgctcgtcg cggcctacga gcccgagatg cgcgcgacga tcagccaggt
     2881 cccggtcgcc gacgggcgcg actggctgca ccgcatgcgc gccgagcacg agtggatcgc
     2941 cttcctcgct tccgtcgagg ccgaccggcg cagccgcgcg gcgacgggcg aggggacctc
     3001 ggtccatccg cgcgagggga tcatggtcgc gactcctgag cgccgcgcga ccaacgtgaa
     3061 ggccgacgtc gacgcgcgca tcccgtccgc ggtcccgctg gccgcggcgc agggcatcct
     3121 cgagtaccgg ccgctggacg tcgcgccacg cgtgcgcggc ctgatggtgg tcgcggtcgc
     3181 cgacgacgcg gtcacgccga cggatcacgc cgtcgcgctg tacgaggccg ccgcgtcgcc
     3241 gaagcagctg gtgatgcagc ggaacacgtc gcactacacg gcgtacacgc agtacgcgag
     3301 cgaggtcatc ccgatgatgg tcgagtggct gcggacgctg gtcctccgtg gcgacgacat
     3361 cgaggtgcgg gccgaccgca cagcgcatgc gagcgttcga tacatcggcg gcagcgaaga
     3421 gggagcgaag gcatgagcag cgacgtcgtc atccgtggcg cctcggtcgt cctcgaggac
     3481 ggcatccgaa aggcggacat cgcgatcgag tccggccgca tctccgcgat cggcgagccc
     3541 ggttcggtcg caacgggaac gcgcgaggtg gacgccacgg acctcacggc gctgcccggg
     3601 atgatcgaca tgcactcgca tcaccgcgag ccgggcttca cccacaagga agacctcgtg
     3661 acgatgggaa agcagtgcgc cgcaggtggc gtcacgacct ccgtggcgat gcccaacgtg
     3721 agcccgcccc ccacgaccgc cgagaacctc gacgcgatgc tcgcggtcta ccgcaagcgc
     3781 gcgtccttcg actacaacgt gaacgcgtcc ggcgtgcagc tggacgagat cccgaagctc
     3841 gccgagcgcg gcatcctcgc gttcaagatc ttcatggtcg tcgacaccgg acggtcatat
     3901 ccgcacatgc ccggcatcgg cctgcacgag cacggcgagc tctacaagcg catgcaggcg
     3961 gtcaagaaga ccggcctgcc gctgatggtc catccgcacg accagtccct gatggacgcc
     4021 atcgagcacg agtactgggc gcgcggagaa cgcgacttcc gcgcgtacgc ccgcgcctat
     4081 gcccacgacg acggcgtcat ctgggacacg gcggtcggcg tgctgctccg gctgcaggcg
     4141 gccaccggtg tgcgtctaca cctcctgcac acccagacgc gccgcgtcgt cgagatgctg
     4201 cgcgcggcga aggccgcggg ccagaccgtg tcggccgagg tgaacccgtg gtgcttctgg
     4261 ctcgacaacg actgggcgaa tatcgagcgg ctcggctcct acgcgctctc gtacttcgtg
     4321 cagccgcatc acgcggcggc ggtgtacgcg gcgttcctcg acggcaccgt cgacatcatg
     4381 gccaccgacc acgcgccgca tctccgcgag gagaaggagc ccgggtggac cgacggctgg
     4441 aaggcgcaca ccggcacgcc gtcggagcag ttctacctct cgctgcttct gaccgacgcc
     4501 gccgcgggca agatcacgct ccagcggatc gcggaggcga cggcgacgcg cccggcgaag
     4561 ctcttcgggc tgtatccgca gaagggcgcc atccgcgtcg gcgccgacgc ggacatcgtg
     4621 ctcgtggacg cgaaggccaa gaggacgatc cgggacgagg acgtgctctc gaagtgcggc
     4681 tggacgccgt acgcgggacg cgaggtccag ggcgtgcccg tctacacgtt cctgcgcggc
     4741 gaggccatct acgacgacgg caagatcgcg atccgcgagg gcttcggccg gcaggcgcgc
     4801 cgcgcgggcg ccgcggtcgc gcggtgacgc gccgccgctc gggcacgccg cggaggcggg
     4861 cgtgacccag gtgtccgtcg cggacagcgc ggccttccgg acgatcgggc cgcggcggcg
     4921 gcagcgcagg aacgtgctgc accgctggta ccgccgcaac gagcgtctgg tgctcagcct
     4981 cttcggcgtc gtcgcgttct tcacgttctg gcaggtcggc gccgacgccg gcatcgtcga
     5041 caagttcttc ttctcgtcgc cgcgtgacat cgtcgtcgcg ggcgccgacg aggtccagaa
     5101 ggcgcgcttc tgggaggacg tctggatcag ctcgttcgag ctggccgtcg gaacggtcct
     5161 cgcgctcatc gcatcggtgc cgctcggcct cgccatcggc tggtaccggc ggctgaacta
     5221 cgcgttcgat ccatggctga acttcctcaa cgcgctgccg cgcgtcgcgc tcatcccgct
     5281 cgtcgtcctc acggcgggcc tcggcgtcga gatgaagatc accgtcgtgt tcctcggcgc
     5341 ggtgttcacg atcatggtcg cgacggtcga gggcgtgcgc acggtggagc ggcagctgct
     5401 cgacgtcgcc cgcagcttcc gcgccccgca gcggcagatc ttcacgtcgc tcgtgatccc
     5461 ggggacgctc ccgttcatcg tgagcggcat gcgcatcgcc gtcggccgcg tgcttgccgg
     5521 cgtgatcatc gccgagttct acgcgcagac gcagggcgtc ggcgtcatga tcctgaaggc
     5581 ctccgccgcg ctgaacagcc cgcgcatgat gttcggcatc ctcctcttca ccctgctcgg
     5641 catcgtcctg tacgaggccg tggggtacgc ggagcggtac ttccagcgct ggcgaccgag
     5701 cctcgatctg gaggagacga cgtgacggat cagcacctgg ccctcaccgc gagcggcgtc
     5761 cagatggaat accgcgatcg tcaaacggga aagcggctcc tcgcgctcga gaacgtcgat
     5821 ctcagcgttg gcgacggcgc gttcgtgagc ctgctcgggc cgagcggctg cggcaagtcc
     5881 accttcctga agatcgtgaa cgggctcctc cgccagacga gcggtgagat caagctgcac
     5941 cgtgtcgcga agggtcgcga ggacgcgatg gtcttccagg acgcctcgct cttcccgtgg
     6001 tacacggtga cggacaacgt cgcctatggc ctcgtctgcg cgggggtccc gcgcaaggtc
     6061 gcgcgccagc gcgcgcagcc gtacatcgat ctcgtcgggc tcaagggctt cggcgacaag
     6121 tacccctacc agctgtccgg aggcatgcag cagcgcgcga acctcgcccg cgccctcgcg
     6181 gtcgactcgg cgatcctcct catggacgag ccgttcgcgt cactcgacgc gcagacccgc
     6241 gagctcatgc aggcggagct gctgcgcatc tggaacgcgt ccaagaagac ggtgctcttc
     6301 gtcacccacc agatcgacga ggccgtcttc ctctccgacc gggtcgtcgt catggccccg
     6361 cggccgggtc gggtcatcgc cgacatcacc atcgacatcc cgcggccgcg cagcctcgac
     6421 gtgaagcgga cgccgcggtt ccaggagtac gagcagaaga tctggcagct catcgcgggc
     6481 cacgtcgagg agaccgccgt cggagcgacg gagccctgat ggcgagctac ccggcgcggc
     6541 ttccgagcgc catgaccagc ccgctcgcgc ggctacgcgg cgcgctcggc gagcgggtgt
     6601 tctacggcct catcggcttc gtcggcgtgc tcatcctgtg ggagacgacg gcccagctcg
     6661 gcctgtaccg caagtcgctc ctcagcacgc cgtcggcgat ctggcgcgtc ggcctcgccg
     6721 acacgacgag cggcatgatc tggcagcaca tctcgatcag catgaccgag ttcgtgatcg
     6781 ggttcgtggt ctcgctcgcc atcggcatcc cgctcggcct ggtcatcggc ctcttcccgc
     6841 gcgtcgacta cctgctcaac ggcctgctcg ccggcctcaa cgcgacgccg aacgtggcgc
     6901 tcatcccgct catcgtgctg ctcgccggta tcggcatgga gtccaagatc gtcgtcgtgt
     6961 tcctgtccgc gttcttcgcc gtcgtcgtca cgacgttcac cggcgtacag gccaccgcgc
     7021 gacggcacct ggagatcacg cggagcttcg gcggatcgca gtggctcgcg ttccgaagcg
     7081 tcgtcctgcc cagcacgctg cctttcatca tcagcggcat ccgcatcggc gcgggccgcg
     7141 cgctcgtcgg cgtggtgagc gccgagttcc tctccgcgaa ccagggtctc ggcttctaca
     7201 tcgccctgta cggacagttc ctcgacaccg cgcgcgtgat gttcggcatc ctcatcttcg
     7261 gcaccctggg gctcgcgatc ggcgagggcg tgcgcttcat cgagcggcgc ttcgaagcct
     7321 ggcgtccgga gctgcaccac taggacggac ggctagtgga aggcgcttcc tccgtccacg
     7381 aggaacgtct ggccggtggt gaacgccgag tcgtctgact gcaggaacgc gatcgtgccc
     7441 acgaggtcca ccggacgctc gaggcgcggg aggcagcggg tcttgatgac gtcctcgcgc
     7501 atcttcacgg tctcgggact cgggttctcc tcggacagcg tgctgcccgg gcacacgagg
     7561 ttcacgcgga tgtagtcctt gcccagctcg cgcgcgatgg tgcggttgaa cccgatgagc
     7621 gacgacttcg cggccacgta gtgcgcgcgc gtcggcacgc cgcggaagat cgtgctcgag
     7681 ccgatgttca cgatgctgcc gcccttgttg gcgcgcaggt gcgggacggc ggccttgcag
     7741 ccgtaccacg cgctcttcga gttcaggcgg aaggactcgt cccattcggc gtcgggaatg
     7801 tcctcgaaat tgcagcgggt gatcgggatc acgttcagca tgcccgcgtt gttcacgatc
     7861 ccggtgagct tgccgaacgt cttcgccgcc tggtcgaccg cgcttttgaa ctgcgcgaag
     7921 tcgcgcacat cggtcgcgat cgcgagcgcg ttgccgcccg aggcgcggat cccctcggcg
     7981 acctcctcga cgcgcttggc gtcgatgtcg agcagcgcca cctgcgcacc ctctgacgcg
     8041 aggcgctcgc tgtacgcccg tccgatcccg tgtgccgcac ccgtgacgac gatcgcctta
     8101 tccgccagac gcatgatccg cattcctcct cgttcgtgtc gacgccgtgg gactccgtgt
     8161 ctcgccgttc acgcgcgcgt ccggcgcacg ccgcccgttc ttctccatcg ccgacgcgtc
     8221 gcgcaggtag cgacggatga ggatggcgac gcgccggtag tggctcgcga gctgtgacgc
     8281 ggcgcgctcg acgtcgccgg cgcgcaccgc ctcgaagaac tgttcgtgat gctcgggggc
     8341 gtgctggagc gtgggccagc gcagcgacct ggcgacctcc tgccgaagcc gcgagatgag
     8401 cgccaccaga cgatgccgtc cggtcatgga gtagagccgg atgtagaagc gctcgcgcgc
     8461 cgcgagctgc gcgtcgccgg gctcggcggc ggtcatctcc gcgtagaggt cctcgagcgt
     8521 ctggaggtcc tcgtcggtca tgagcggcac ggcgaggcgc acggcgtggc tctcgagcag
     8581 gacgcggagg tcgtagacct cctcgatgtc gtccgcgtcg agctccgtca cggtgaagcc
     8641 gcggtagggg agcgaggtga cgagtccctc gtactcgagc gcgcggagcg cctcgcggac
     8701 cgggatccgg ctcgtgccga acatctcggc gagcttctcc tggcgcagac gcgtgcccgg
     8761 ggcgagcgtt ccggtgagga tcgcctcgcg catggaggtc aggacgaggt cctgggccgt
     8821 gagcttgcgt gccgccgact cggcgatgat gcggtcgagc cgtgccttga cgtccgtatc
     8881 gccgccgttc aggccgccca tcgtcgtccc cttccccgcg cgccgcgccg cgggccgatc
     8941 atggccctgg cctccacgtc cgtcccgatt gtattcacga tgaccctgcg gttgaacgga
     9001 atgggtccgc atgcagacaa tgcaggtaca tgaggtctcg ggtcggcccg ttcgcccaga
     9061 gggcgtacac cgtctactgg ggcggcggtc tcgtatcgaa catcgggacc tggcttcaga
     9121 acgtcgtcgc ggcggtcttc gtgtacgacc gcaccggctc cgcgctcgcc gtcggcctgc
     9181 tcagcttcgc gtcgtggctg ccgatgctgc tgttctcggt ccacggcggc atgctctcgg
     9241 accgcttcga ccgccggatc atcgcgatcg tcacgcagac gatctcgctc gtcgtgagcg
     9301 cggtgctcgc ggtgctcgcg atcaccggca atgccaacga gtatcacgtc gccatcaccg
     9361 cgttcctgct gcagacgtcg tggacgatcg cgaagccgtc ggtgacggcg atgatccccg
     9421 ccctcgtcga gcgcgagcag ctcaccgagg ccgtgggcct caacacgctc cagttccaga
     9481 tcgcccagct cggcggtccg gtgctcgcga ccgtgctgct cacgacgtcg gggtacgcgt
     9541 gggcgttcgc gatcaacgcc gtgtcgttcg tcgcgccggt gctcgcgatg gcctacctct
     9601 acgtccgcgg catcggcgga cccgggtcgc gggccgcgag aaaggcccgc gccgacgcga
     9661 ccgggatcgt ggactacgtg cggggccagc aatggatcgc gtgggtgatg gtcggggtga
     9721 tctcgacgag cgccgtggcc gagatgatcc gcacgctctc acccgtcatc gtcacgcacc
     9781 tcggcgcgcc gtcgagcgac acgggcctct tcgtcgcggc gcagagcgtg ggcatggtcc
     9841 tcgggatctt cacctcggtg cccctcacgc ggcgcggcct cgcgcgcagc ctcgcgccgg
     9901 tcgggctcgt ctttcaggcc gtcgggctcg tcgtggtggc gacggcgccg agcatgctcg
     9961 tcgcgctgat cggcgggggt ctcgtcggcg gcggcttctc gtggtgcttc cccgtgctca
    10021 ccggcgtgct gcagacggag gtgcccgacg ccgtgcgcgg ccgcctgctc gcgctgcacc
    10081 agatggcgca cctcgggaac cggccgttcg ccgccctcgc cgccggggcg atcgcggccg
    10141 ccttcggcgc gccggtcgcg tgcctcgccg gcgtgctgct cgccccggtg ggactcgtcg
    10201 cggtgcgatc ggcctggcgg aggctcgacc acgcggcggt gcccgagccc gtgatcgcgg
    10261 aatcggcgtg agcgtccgcg tgagcgtcga ccccgacctc tgcatcggca acgccgagtg
    10321 cgcgcggatc gcgccggccg cgttccgcct cgacgacgcg cgcggcgtct cggtcgtgct
    10381 gcccggcgcc tcgtccgtcg atcgcgaggt cctcgaggag gccgcattca actgtccgac
    10441 gcgcgcgatc acggtcacgg acgcgccgga gtgagcgacg agggcctgct cacgcggtac
    10501 accgcgacga tcacgttcga ccgctacgag gacatccgcg cggcgctctt cgatccgcac
    10561 ctgtcgcgca ccttcgacaa gcgctcgtac gagggcggca acatccgcga cgggatcgtg
    10621 agcatcgctc acggtgcgac gcaccgggcg cgccgacgca tcgagaacac gcggttccgc
    10681 gccgaggtcc tgcgcctcta cgagcgcgag ctgttcccgg gggtcatgaa cgagctcctg
    10741 gacacgctca tcacgagcga tcgcgtggat ctcttcccca tcggcgagat gctctcggtc
    10801 gtcctggcgg cgaggcgcgc gggcatcgag tacgacccct cgtcgctcga cgacctgcgc
    10861 acgctcgtgc gacacgtgga cgcgttctcc cagggcagcg cgatcctcga cgccaaggat
    10921 cccgacgccg tgcgcgcgct ggtgcgcgcc gcctacgagg acttcgagcg tgacttcgta
    10981 cgcccggcgt gggagcggcg cgcggagctc atcgacgcgg tccggcgcgg cgaggctcgt
    11041 gagcaggatc tgccgcagga catcctcacg gtgctgctgc tccatcgcgc cgatccgagc
    11101 ctcgagctcg gcgacgacgg tcgtgtcgtg cgcgaggtcg ccacgtacct gcagggcggc
    11161 acgcatacga gcgcgcagac gctggtgaac tccctcgacc tgatgttcgc ggaccccgac
    11221 cccgcgcggc tgctcgagcg cgtcgcgcgc gacacgctct tcgcgcagcg cgtcgtgcag
    11281 gagacgctcc ggctccgtcc cacgacgccg aaggcgaagc ggcgcgctga agcggacacc
    11341 gaggtcgccg ggcgccggat tccgaaagac gcgctcgtcg tgctcgacct tgcgagcggc
    11401 aaccgcgacc cggaggtctg gggcgcgacc gcggaccagt tcgatccgga ccgcacggtg
    11461 cccgcgaacg tgccgcgctg gggcctctcg ttcggcgccg gcgcgcatca gtgccccggc
    11521 cgcgccgtcg gcggcggctt cccgatcccc gcttcattcg atgtggacga cgaccacg
//
LOCUS       DASTZR010000345         8229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_354807, whole genome shotgun sequence.
ACCESSION   DASTZR010000345 DASTZR010000000
VERSION     DASTZR010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354807"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VFV20_07725"
     CDS             complement(<1..314)
                     /locus_tag="VFV20_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018828459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU5288275.1"
                     /translation="MSGEREVRVAIDAARTGGTALRARWSDRAAGLGTKTTSTDVVLD
                     ADRASEAEVVRVLRSAFPGDAIVAEEGTDIGAGAVRRWYVDPLDGTVNYLYGIPHFAV
                     AIA"
     gene            complement(361..1899)
                     /locus_tag="VFV20_07730"
     CDS             complement(361..1899)
                     /locus_tag="VFV20_07730"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HEU5288276.1"
                     /translation="MRGLLIAVLLVLASACTSTGVPIESPSAAAALAPDRIQLLHTDD
                     IHGRLDAEKVQGGSATFDQGGMAALASQVYAQRRRAPQRTLLLDGGDAWQGTFISNAN
                     KGEAVTRAMTLMGYDAMAVGNHDFDWGQDVIAQRAKEASFPFLATNVVETATGKLPAY
                     LKPYVVKDLGIARVGILGVTNPLSNTIVKATSVAGLRFGPASDVQRYLPEVQAQADIV
                     VVVAHIGSADAMMLARDVPGIDVIVAAHDHASVQTARLEGRTTIVNAGAYTQYLGRLE
                     IVVDPATRKMKDAVRAGALIAVAASSSLRPDAEITGLVEARRAEAEKYTSRVVGTIKA
                     DLDNPRDENGLGNMITDAFVEYGRQQGWKTDVAFYNAAGVRAPLKAGPVTYGLLYQVL
                     PFGNTIVSVDLTGEQLREVLEDASGRAGRVQIGGGRWVYRFTNDAGQRVLEATIAGQP
                     LDPRRVYHVATIDYLLLGGDGHTWLGKGTNVIYGDIEVDVVASYMTAHSPLDPRVEGR
                     ITTR"
     gene            complement(1928..3775)
                     /locus_tag="VFV20_07735"
     CDS             complement(1928..3775)
                     /locus_tag="VFV20_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5288277.1"
                     /translation="MAGHHGGGSGGFFHEDEILGKAYDAALTRRLVSYLMPYRLWVGI
                     ALVLLLGQSLAQIAPPVLAKEIVDQAVVPAVNGTIPQGDAFARLGLLGVGYLLVLGAG
                     AGLRYGQTLLMTWVGQRAMYDLRLELFRHLQFLPLAFFDRNPVGRLMTRITNDIDALT
                     EMVTRGVVSIFGDIAMIILIAIAMLLLDWRLAVVTFLTLPILVAVTAYFRGMMRESFR
                     AIRIRLARVNAYLNETLSGMAIVQLFTRERPTFEQFDALNRDLLSANQGQVRAMSVFQ
                     PTVNFTRNATAALLFVVGANWILQRELSLGTLVAFWQLLQQFFQPILDLSEKYNIMQA
                     AMASSERVFRLLDTRSNILDPDHPVELPHVRGEISFDGVSFAYNEGDWVLRDVDFKIS
                     AGESVAIVGATGAGKTSIISLISRFYDVQKGRILLDGVDIRDMRQRDVRRHVGVVLQD
                     PFIFAGTIASNIRLNETGITDEQVRAAARFVNADKFIERLPSGYDEVVTERGSTLSVG
                     QKQLLAFARAIAFNPEILLVLDEATSSVDTETEHLIQDALAKLMVGRTSIIIAHRLST
                     IQNVDRIVVLHKGRVVEMGTHRDLLAQRGVYHRLYELQYRAHEQVAGRG"
     gene            complement(3775..5622)
                     /locus_tag="VFV20_07740"
     CDS             complement(3775..5622)
                     /locus_tag="VFV20_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5288278.1"
                     /translation="MTSSRATLLHYLGQHKLRLATGVVVVAISAFVAVLPPLLIGRIV
                     DALTTGAGLDSVIRLAILMVVLAGVENVFRGLGRWRILDASRQIEYRMRNDLLAHLQT
                     MHLAYFQHQRIGDLMARLTNDLQAVRQMIGFGILMLTTTLLTLLFTFVSMFGVDTKLA
                     LITLALTPISSYLFWTIGRRVNRRFEQLQSQFGDLSAKAQENFSGIRVVKAFSQEEPE
                     IGAFAKVNRDYLDAAVGLARVNGSLWPTMQFILGAAVLAILYIGGQDAIAGHLTIGQL
                     VQFIAYLQIISWPMIALGEVVNLVQQGWASLGRLEDIYESRPTVAEPIDPVNARISGA
                     VEFRNVSFAYGPAIVLRDVSFRVPAGGSLAIVGPTGAGKSSLVNLIPRLFDAQQGEIL
                     IDGVDVRRYPLPLLRRAVGYVPQETFLFSVPLRENVGFGTDRPLTDTELEWAGEVSQL
                     GKDVEDFPARYDTMIGERGVTLSGGQKQRTAIARAVAKDPRILILDDALSAVDTYTEA
                     EILKRLRGVMRERTSIVVAHRISTVKDADEILVLDDGRIAERGTHGELLALGGLYAAM
                     YRRQLLEEELQVDEEQSEHEKRVRAADEPRADTYGRRGPRMDDMAGGQS"
     gene            complement(5634..6989)
                     /locus_tag="VFV20_07745"
     CDS             complement(5634..6989)
                     /locus_tag="VFV20_07745"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5288279.1"
                     /translation="MAEVTEAAQHGLLRNRAFTALWAAQVLSQTAANAVTSALIILVA
                     ELTHSNTSSSFLILLAIIPAVLFGFAGGVIVDRSDRRLVLVITNALRAVAIIPLVLAG
                     ESVTTAYLVNFLVATVTIFFVPAEAATIPSIVRKRDLLAANSLFTFTFNGAFLIGFII
                     LAPIVVNLYGFYVLWFTVAAMFAVASLLCLTLPPAPKAASLLSVELAERAVSETRRGI
                     SEAFHYLRSTPLVTWAMVYIALTYTLVAIAGALAPGVVREVLRLGERNVVVLVGPAGV
                     GVVAGLGLLNVASRRIGRSHAIGAGLMVTVIALVVLAAARPFADVFAESRFGGAIGSL
                     GGALPFFVGIVSVTAFVFGVAYSFITVPSMTLLQEELPEDIRGRVFGVLNSLVSIFSF
                     LPLIIVGPIADVWGVAPVFVLAAAIVLGMWMAGRAERLRARLGTSPNVTNITERRESS
                     G"
     gene            complement(6998..7234)
                     /locus_tag="VFV20_07750"
     CDS             complement(6998..7234)
                     /locus_tag="VFV20_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEU5288280.1"
                     /translation="MSATRTSGAKGPLALYLDDVIVLRKPHPCGSDTWRIVRLGADIG
                     VVCAGCKRRVLVPRAALERDIKRFAERGPLAQAD"
     gene            complement(7259..7906)
                     /locus_tag="VFV20_07755"
     CDS             complement(7259..7906)
                     /locus_tag="VFV20_07755"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEU5288281.1"
                     /translation="MSTNAGGIATTIVALFLGLLAVTQVRTQDVYSRSLELETPASLT
                     TLIANVAERNNALREEIFDLRLRTESARDDAASGKGSLTEAQRQLSQLQVFAGRTAVK
                     GQGITVRVDGAFDDKALSDLVNELRNAGAEAIAINSVRVGPRSWFGVTPDRALTVDAQ
                     PIRGPWTVSAVGAPEVMYVAMTRTGGIIGQFELIYRNTRFSVVKETALDLPTLGR"
     gene            complement(7903..>8229)
                     /locus_tag="VFV20_07760"
     CDS             complement(7903..>8229)
                     /locus_tag="VFV20_07760"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEU5288282.1"
                     /translation="TDIINTAWRGGAQAIAVNEERIVGSSSVYCVGSTIMVNGTLMSP
                     PFSIVVIGSQNDLLSGFDDPGELRDIKQRRDVYGLGFRVTRATNVRVPAYGGALNVRY
                     ATPQQP"
BASE COUNT         1341 a         2806 c         2830 g         1252 t
ORIGIN      
        1 gcgatcgcga cagcgaagtg cgggatgccg tagaggtagt tcaccgtgcc gtcgagcggg
       61 tccacgtacc accgtcgcac ggcgcccgcg ccgatgtcgg tgccctcctc ggcgacgatc
      121 gcgtcgcctg ggaacgccga ccggagcacg cgcacgacct cggcctcgga ggcacgatcg
      181 gcgtcgagca cgacatccgt gctggtcgtc ttcgtgccga gcccggccgc gcggtcggac
      241 caccgagctc gcagcgccgt gccgccggtg cgcgccgcat cgatcgcgac gcgtacctcg
      301 cgctcgccgc tcacgagggc agcaggcggc cggcggcagg gcccgctgcc gtcatgagcc
      361 ctatcgcgtc gtgatccttc cttcgactct cgggtcgagc ggcgagtgcg ccgtcatgta
      421 cgaggcgacg acgtctacct cgatatcgcc gtagatcacg ttcgtgccct ttccgagcca
      481 ggtgtgtccg tcaccgccga gcaggaggta gtcgatcgtc gcgacgtggt acacgcgcct
      541 cgggtcgagc ggctgcccgg cgatcgtggc ctcgagcacg cgctggcccg cgtcgttggt
      601 gaaccgatag acccaccttc caccgccgat ctgcactcgt cccgcgcgac cggacgcgtc
      661 ctcgagcacc tcccgcagct gctcgcccgt gagatcgacg ctcacgatcg tgttgccgaa
      721 cgggagcacc tggtacagca ggccgtaggt cacgggaccc gccttcagag gcgcgcgcac
      781 gcccgcggcg ttgtagaacg cgacgtcggt cttccagccc tgctgccttc cgtactccac
      841 gaacgcgtcc gtgatcatgt tgccgaggcc gttctcgtcg cgcgggttgt cgaggtccgc
      901 cttgatcgtg ccgacgaccc gagacgtgta cttctcggcc tccgcgcgtc gcgcctcgac
      961 cagccccgtg atctccgcgt ccggtcgcag cgagctggaa gccgccaccg cgatgagcgc
     1021 gccggcgcgg accgcatcct tcatcttgcg cgtcgcgggg tcgacgacga tctcgaggcg
     1081 cccgaggtac tgcgtatacg cgccggcgtt gacgatcgtc gtccgcccct cgagccgcgc
     1141 cgtctgcacg gacgcgtgat cgtgggccgc gacgatgacg tcgatcccgg gaacgtcgcg
     1201 ggcgagcatc atcgcgtcgg ccgagccgat gtgggccacg accacgacga tgtcggcctg
     1261 ggcctgcacc tcgggcagat accgctgcac gtcgctcgcg gggccgaacc tcagtcccgc
     1321 gacgctcgtc gccttcacga tcgtgttcga cagcggattg gtgacgccga ggatcccgac
     1381 cctggcgatg ccgaggtcct tcacgacgta gggcttgaga tacgccggga gcttgccggt
     1441 cgccgtctcc acgacgttcg tcgcgaggaa cgggaatgag gcttccttcg cccgctgcgc
     1501 gatcacgtcc tggccccagt cgaagtcgtg gttgccgacg gccatggcgt cgtagcccat
     1561 gagcgtcatc gcgcgggtga cggcctcgcc cttgttcgcg ttcgagatga aggtgccctg
     1621 ccacgcgtcg ccgccgtcca ggaggagcgt gcgctgggga gcgcgccgtc gctgcgcgta
     1681 cacctgcgac gcgagcgcgg ccatgccgcc ctgatcgaag gtcgccgacc cgccctgcac
     1741 cttctccgcg tcgagccgac cgtggatgtc gtcggtgtgc agcagctgga tccggtcggg
     1801 cgcgagcgcg gcggccgccg atggcgactc gatcggcacg ccggtcgacg tgcacgccga
     1861 cgcaaggacg aggaggaccg cgatcaggag tccacgcacc gccgtggatg ctagcggcac
     1921 ggcgttgcta cccgcgcccg gcgacctgct cgtgtgcccg gtactgcagc tcgtacagcc
     1981 ggtggtacac gccgcgctgc gcgagcagat cacggtgcgt gcccatctcc accacgcgac
     2041 ccttgtggag cacgacgatg cggtcgacgt tctggatcgt cgaaaggcgg tgggcgatga
     2101 tgatcgacgt ccgtccgacc atgagcttcg ccagcgcgtc ctggatgagg tgctcggtct
     2161 ccgtgtccac ggagctcgtc gcctcgtcga gcaccagcag gatctcgggg ttgaacgcga
     2221 tcgcgcgcgc gaacgcgagc agctgcttct gccccaccga gagcgtcgag ccccgctcgg
     2281 tcacgacctc gtcgtagccg ctcgggagcc gttcgatgaa cttgtccgcg ttcacgaagc
     2341 gcgccgcggc gcgcacctgc tcgtcggtga taccggtctc gttcagccgt atgttcgagg
     2401 cgatcgtgcc ggcgaagatg aacgggtcct ggagcaccac gccgacgtgc cggcgcacgt
     2461 cccgctgccg catgtcgcgg atgtcgacgc cgtcgaggag gatcctcccc ttctgcacgt
     2521 cgtagaaccg cgagatgagg ctgatgatcg aggtcttgcc agcaccggtc gcgccgacga
     2581 tggccacgct ctcgcccgcc gagatcttga agtccacgtc gcgcagcacc cagtcgcctt
     2641 cgttgtacgc gaacgacacg ccgtcgaagc tgatctcgcc gcgcacgtgc ggcagctcca
     2701 ccgggtggtc cgggtcgagg atgttcgacc gcgtgtcgag cagccggaag acgcgctcgg
     2761 acgacgccat cgcggcctgc atgatgttgt acttctcgga gagatccagg atcggctgga
     2821 agaactgctg cagcagctgc cagaaggcga cgagcgtccc aaggctgagc tcgcgctgga
     2881 ggatccagtt cgcgcccacg acgaacagca gcgccgccgt cgcgttgcgc gtgaagttca
     2941 cggtgggctg gaacacgctc atcgcgcgca cctgtccctg gttcgcggac aacaggtcgc
     3001 ggttcagcgc gtcgaactgc tcgaacgtcg gccgctcgcg tgtgaacagc tgcacgatcg
     3061 ccatgcccga gagcgtctcg ttgagatagg cgttcacgcg cgcgagccgg atgcggatgg
     3121 cgcggaacga ctcgcgcatc atgccgcgga agtacgccgt gaccgcgacg aggatcggca
     3181 gcgtcaggaa ggtcacgacg gccagccgcc agtcgaggag cagcatggcg atcgcgatga
     3241 ggatgatcat cgcgatgtcg ccgaagatcg acacgacccc gcgcgtcacc atctcggtga
     3301 gcgcgtcgat gtcgttggtg atgcgggtca tgagccggcc gacggggttg cggtcgaaga
     3361 acgcgagcgg caggaactgc agatgccgga agagctcgag gcgcaggtcg tacatcgcgc
     3421 gctgccccac ccaggtcatg aggagcgtct gcccgtagcg cagtccggcg ccggcgccga
     3481 ggacgaggag gtacccgacg ccgagcagcc cgagacgcgc gaacgcgtcg ccctgcggga
     3541 tcgtgccgtt caccgcgggc acgaccgcct ggtccacgat ctccttcgcc aggaccggcg
     3601 gggcgatctg cgccagcgat tggccgagca gcagcaccag cgcgatcccg acccagaggc
     3661 gatagggcat gaggtacgag acgagccgcc gggtcagggc ggcgtcgtag gccttgccga
     3721 ggatctcgtc ctcgtgaaag aacccgccgc tcccgcctcc gtggtggccg gccatcagga
     3781 ctgccctccg gccatgtcgt ccatccgtgg gccgcggcgc ccgtacgtat cagcccgcgg
     3841 ctcgtccgcg gcgcggacgc gcttctcgtg ctcggactgc tcctcgtcca cctgcagctc
     3901 ctcttccagg agctgccggc ggtacatggc cgcgtagagc ccgcccagcg cgagcagctc
     3961 gccgtgcgtg ccgcgctccg cgatgcggcc gtcatccagg acgaggatct cgtccgcgtc
     4021 cttcacggtc gagatgcggt gcgcgacgac gatgctcgtc cgctcgcgca tgacgccgcg
     4081 aaggcgcttc aggatctcgg cctccgtgta cgtgtcgacg gcggacagcg cgtcgtcgag
     4141 gatgaggatg cgcgggtcct tcgcgacggc acgggcgatc gcggtgcgct gcttctggcc
     4201 acccgacagg gtcacgccgc gctcgccgat catcgtgtcg tagcgagctg ggaagtcttc
     4261 gacgtccttt ccgagctgcg acacctcccc cgcccactcg agctccgtgt cggtcagcgg
     4321 ccgatcggtg ccgaagccga cgttctcacg aagcggcacc gagaagagga acgtctcctg
     4381 cggcacgtat ccgaccgcgc gtcgcaggag cggcagcggg taccgccgca catcgacgcc
     4441 gtcgatgagg atctctccct gctgcgcgtc gaagaggcgc gggatgaggt tcaccagcga
     4501 gctcttgccc gcgcccgtcg ggcccacgat cgcgagcgat ccgccggcgg ggacgcggaa
     4561 cgacacgtca cgcagcacga tcgccggtcc gtacgcgaag ctgacgttcc ggaactcgac
     4621 ggcgccggag atcctggcgt tcaccgggtc gatcggctcc gcgaccgtcg gccgtgactc
     4681 gtagatgtcc tcgagccggc cgagcgacgc ccagccctgc tgcacgaggt tcacgacctc
     4741 gccgagcgcg atcatcggcc acgagatgat ctgcaggtac gcgatgaatt gcacgagctg
     4801 cccgatggtg aggtggccgg cgatcgcgtc ctgccccccg atgtagagga tcgcgaggac
     4861 cgccgcgccg aggatgaact gcatcgtcgg ccacagcgag ccgttcaccc ttgccagtcc
     4921 gacggcggcg tcgagatagt cgcggttcac cttcgcgaag gcgccgatct ccggctcctc
     4981 ctgtgagaac gccttcacga cgcggatccc cgagaagttc tcctgcgcct tcgccgagag
     5041 gtctccgaat tgcgactgca gctgctcgaa gcgtcgattc acgcgccgcc cgatcgtcca
     5101 gaagagatag gacgagatcg gtgtgagggc gagcgtgatg agcgcgagct tcgtgtcaac
     5161 gccgaacatc gagacgaagg tgaagagcag ggtcagcaga gtggtcgtca gcatgaggat
     5221 gccgaagccg atcatctgac gcaccgcctg caggtcgttg gtgagacgcg ccatgaggtc
     5281 gccgatgcgc tggtgctgga agtacgcgag gtgcatggtc tggaggtgcg cgagcagatc
     5341 gttgcgcatg cggtactcga tctggcgcga cgcgtcgagg atccgccacc gaccgaggcc
     5401 gcggaacacg ttctcgacgc ccgcgaggac gaccatgagg atggcgagcc ggatcacgct
     5461 gtcgaggccg gcacccgtcg tcagagcgtc gacgatgcgg ccgatgagca gcggcggcag
     5521 cacggccacg aacgcgctga tggcgacgac gacgacgccc gtggcgagcc ggagcttgtg
     5581 ctgtccgagg taatgaagga gcgtggcccg ggaggaggtc acccgaccga agtttacccg
     5641 ctcgactcgc ggcgctccgt gatattggtc acattcggac tggtcccgag gcgcgcgcgg
     5701 agccgctcgg cgcgaccggc catccacatg ccgagcacga tcgccgcggc gaggacgaac
     5761 acgggcgcca cgccccacac gtccgcgatc ggtccgacga tgatcaaggg caggaacgag
     5821 aagatcgaca ccagcgagtt cagcacgccg aacacccggc cacggatgtc ttcgggcagc
     5881 tcctcctgca ggagcgtcat cgacggcacc gtgatgaacg aataggccac accgaagacg
     5941 aacgccgtga cgctgacgat gccgacgaag aacggcagcg cgccgccgag gctgccgatc
     6001 gcgccgccga accgcgattc cgcgaacacg tcggcgaagg gacgcgccgc cgccagcacg
     6061 acgagtgcga tgaccgtgac catgagcccg gcgccgatgg cgtgcgaccg tccgatgcgg
     6121 cggctcgcga cgttcagcag gccgaggccg gccacgacgc cgacgccggc gggcccgacg
     6181 aggaccacca cgttgcgctc gcccaggcgg aggacctcgc gaacgacgcc tggggcgagc
     6241 gcgccggcga tcgccaccag cgtgtacgtc agcgcgatgt agaccatcgc ccacgtgacc
     6301 agcggcgtgg atcggaggta atggaacgcc tcgctgatgc cgcgacgagt ctcgctgacg
     6361 gcgcgctccg cgagctcgac ggagagcagc gacgccgcct ttggcgcggg cgggagggtc
     6421 aggcagagca acgacgcgac ggcgaacatc gccgcgaccg tgaaccacag cacatagaag
     6481 ccgtagaggt tgacgacgat gggcgccagg atgatgaagc cgatgaggaa cgcgccgttg
     6541 aacgtgaacg tgaacagcga gttcgcggcg aggagatcac gcttgcggac gatcgaaggg
     6601 atcgtcgccg cctccgcggg cacgaagaag atcgtgaccg tcgcgacgag gaagttcacg
     6661 aggtaggcgg tcgtcacgct ctcgccggcg aggacgagtg ggatgatcgc gacggcacgc
     6721 agcgcgttcg tgatcacgag cacgaggcgc cggtcgctgc ggtccacgat cacgccgccg
     6781 gcgaatccga agaggacggc ggggatgatg gcgaggagga tgaggaagga ggacgacgtg
     6841 ttcgagtgcg tcagctcggc cacgaggatg atgagcgccg aggtgaccgc gttcgccgcc
     6901 gtctgggaca ggacctgagc ggcccacagc gccgtgaatg ctcggttccg gagcaggccg
     6961 tgctgcgcgg cctcggtgac ctccgccaag agccggctta gtccgcctgc gccaacgggc
     7021 cgcgttccgc gaagcgcttg atgtcgcgct ccagcgccgc gcgcggcacg agcacgcgcc
     7081 gcttgcagcc cgcgcagacc acgccgatat ccgcccccag acggacgatg cgccaggtgt
     7141 cgctgccgca cgggtgcggc ttgcgaagca cgatcacatc gtcgagatac agggcaagcg
     7201 gtcccttcgc gccacttgtg cgtgtcgcgc tcatgaccgg cagtctagcc gcgcagccct
     7261 atctcccgag cgtcgggagg tcgagcgccg tctccttgac gacgctgaac cgcgtgttcc
     7321 gatagatgag ctcgaactgt ccgatgatgc cgccggtcct ggtcatcgcg acgtacatga
     7381 cctccggcgc acccacggcg ctcacggtcc acggaccgcg tatcggttgc gcgtcgaccg
     7441 tcagtgcccg atccggtgtc acgccgaacc acgatcgcgg accgacgcgc acgctgttga
     7501 tcgcgatcgc ctccgcgccc gcgttgcgca gctcgttgac caggtccgag agtgccttgt
     7561 cgtcgaatgc gccgtcgacg cgaacggtga tgccctggcc cttgaccgcc gtccgcccgg
     7621 cgaagacctg cagctgcgac agctgtcgct gcgcctcggt gaggcttccc ttgccgctcg
     7681 cggcatcatc gcgtgcgctc tcagtgcgga gccgcagatc gaagatctcc tcgcgcagcg
     7741 cgttgttcct ctcggcgacg ttcgcgatga gcgtcgtgag cgacgcaggc gtctcgagct
     7801 ccaggctgcg gctgtatacg tcctgcgtgc gcacctgcgt caccgccagc aggccgagga
     7861 acagcgcgac gatcgtcgtg gcgatcccgc ccgcgttcgt gctcacggct gctgaggcgt
     7921 ggcgtagcgg acgttgagcg cgccgccgta cgcggggacc ctgacgttcg tggcgcgcgt
     7981 cacacggaag ccgagcccgt acacgtcacg ccgctgtttg atgtcacgca gctcgccggg
     8041 atcgtcgaat cccgacagca gatcgttctg cgacccgatc acgacgatcg agaagggtgg
     8101 ggacatgagc gtgccgttca ccatgatcgt cgagccgacg cagtacacgc tcgacgatcc
     8161 gacgatgcgc tcctcgttga cggcgatcgc ctgcgcgccg ccgcgccacg cggtgttgat
     8221 gatgtcggt
//
LOCUS       DASTZR010000346         2227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_306830, whole genome shotgun sequence.
ACCESSION   DASTZR010000346 DASTZR010000000
VERSION     DASTZR010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306830"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VFV20_07765"
     CDS             complement(<1..437)
                     /locus_tag="VFV20_07765"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEU5288283.1"
                     /translation="MVTRDALREVLRRHPSGVAVVTVEANAQRIGLTVATLTSLSLDP
                     PLVGVAIAREAALHELLREAEAFAVSFLAADQGHVAEHFARGVPPIAMWKGVAAREGE
                     LGAPLLDGALGWLECRLADALPVGTHTFFVGEVVSATEGEDGVP"
     gene            492..767
                     /locus_tag="VFV20_07770"
     CDS             492..767
                     /locus_tag="VFV20_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEU5288284.1"
                     /translation="MCRNIKTLHNFDPPATDEEVRAAALQYVRKISGSAKPSKANEAA
                     FERAVAEVTESSLRLLADLVTVAPARNREIEAAKARARAEKRFGAAA"
     gene            842..>2227
                     /locus_tag="VFV20_07775"
     CDS             842..>2227
                     /locus_tag="VFV20_07775"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEU5288285.1"
                     /translation="MAVAAALPAVAMLLGGAHHIQVDAWIHFVGVGFAAAIAMGAALV
                     LTVLGALQSDGRAVLAGLAFSIMAALLCLHGASTPGILVGSNGVVAFTGGATLPVGCA
                     ILALGTLSALRRPEAVKPLLVVLALGATAILALGISMIHWPGLVPSVPEPRSLLALTV
                     LAAGLVATALLVVRALRTYLLTRRFLDLVVIVGIAWLGTALVASLVFSFQNIGWWFGH
                     VLEIFGMALVGIPVALDLRRGATHQSRPLVGDLRAVDLVSAEEAFLGSQVRALLVALA
                     VKDGSTEEHTRRVALLAVEVGDELGLPPGRLRSMATGGLLHDIGKLSVRDEVLRKPAS
                     LTDAEFAEVKQHPGAGVALLEELGGFDPLVLALVNDHHERLDGSGYPRGLADGEISLE
                     AQILAVCDVYDALISPRVYRDAWPHERAMALLRQGAGLHFDARCVDALERVLTRDQAE
                     RRAARQTGPEVS"
BASE COUNT          277 a          837 c          783 g          330 t
ORIGIN      
        1 gggaccccgt cttcgccctc ggtcgcggag acgacctcgc cgacgaagaa cgtgtgcgtg
       61 cccaccggca gcgcgtcggc cagtcggcac tcgagccagc cgagcgcgcc gtcgagcagc
      121 ggcgcgccga gctcgccttc ccgtgccgcg acgcccttcc acatcgcgat cggcggcacg
      181 ccgcgggcga agtgctccgc cacatggccc tggtccgccg cgaggaagga gaccgcaaac
      241 gcctcggcct cacgcaggag ctcgtggagc gccgcctcgc gcgcgatcgc aacgccgacg
      301 agcggtgggt cgagcgagag cgacgtgagc gtcgcgacgg tcaggccgat gcgctgggcg
      361 tttgcctcga cggtcaccac ggcgacgccg ctcggatggc ggcggaggac ttcccggagc
      421 gcgtcgcgcg tgaccacgac cggactctac gaccgccggg agctcgaacg gccgcaggta
      481 ggctcgacgc catgtgcagg aacatcaaga cactccacaa cttcgacccg cccgccaccg
      541 acgaggaggt ccgcgccgcc gcgctgcagt acgtccgcaa gatcagcggc tcggccaagc
      601 cgtcgaaggc caacgaggcc gcgttcgagc gcgcggtcgc cgaggtgacc gagtcctcgc
      661 tgcggctgct cgccgacctc gtcaccgtcg cgccggcgcg gaaccgcgag atcgaggccg
      721 cgaaggcgcg ggcgcgggcc gaaaagcgct tcggcgcggc cgcctagccg ccctcaaccc
      781 gagcgccgca gctgccgata tcccatcggt ggcagctcgg cgctcggctg cgctcctctt
      841 catggcggtg gcggcggcac tgcctgctgt cgccatgctc ctcggcggcg cccaccacat
      901 ccaggtcgac gcctggatcc acttcgtcgg cgtcggcttc gcggctgcga tcgcgatggg
      961 ggcggcgctc gtgctcaccg ttctcggcgc gctccagtcc gacgggcgcg cagtgctcgc
     1021 ggggctcgcg ttctcgatca tggcggcgct cctctgcctc cacggcgcct cgacccccgg
     1081 gatcctcgtc gggtcgaacg gcgtcgtcgc gttcacgggc ggtgcgacgc tgccggtcgg
     1141 gtgcgcgatc ctcgcgctcg gcacactgtc cgcactccgt cgtccggagg cggtgaagcc
     1201 cctactcgtc gtgctcgcgc tcggagcgac ggcgatcctg gcgctcggca tctcgatgat
     1261 ccactggccc ggcctcgtcc cctccgttcc cgagccgcgg agcctgcttg cgctcaccgt
     1321 gcttgcggcg ggtctcgtcg ccaccgcgct gctcgtcgtg cgcgcgctgc gcacctacct
     1381 gctcacacgc cggttcctcg acctggtcgt catcgtcggc atcgcgtggc tcggcacagc
     1441 gctcgtcgca tcgctcgtct tcagcttcca gaacatcggc tggtggttcg gccacgtcct
     1501 cgagatcttc gggatggcgc tcgtcgggat cccggtcgcg ctcgatctgc gccgcggcgc
     1561 gacccatcag tcgcggccgc tcgtcggcga cctgcgcgcc gtcgacctcg tctccgccga
     1621 ggaggcgttc ctcggctcgc aggtgcgcgc gcttctcgtt gcgctcgcgg tcaaggacgg
     1681 ctcgaccgag gagcacacgc gccgggtggc gctgctggcg gtcgaggtgg gcgacgaact
     1741 ggggctgccg ccgggccggc tccgctcgat ggcgaccggc ggactgctcc acgacatcgg
     1801 gaagctctcc gtacgcgacg aggtgctgcg caagccggcg tcgctgaccg acgcggagtt
     1861 cgcggaggtc aagcagcacc ccggcgcggg cgtcgcgctg ctggaggagc tcggcgggtt
     1921 cgacccgctc gtgctggcgc tcgtcaacga ccaccacgag cggctcgacg gctcgggcta
     1981 tccccgcggc ctcgcggatg gggagatctc gctcgaggcc cagatcctcg cggtctgcga
     2041 cgtctatgac gcgctcatct cgccgcgcgt ctaccgtgac gcctggcccc acgagcgcgc
     2101 gatggcgctg ctccgacagg gcgccggact gcacttcgat gcgcggtgcg tcgatgccct
     2161 ggagcgggtg ctcacgcgcg accaggcaga gcgacgggcg gcccggcaga ccggccccga
     2221 ggtctcc
//
LOCUS       DASTZR010000347         2847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_773692, whole genome shotgun sequence.
ACCESSION   DASTZR010000347 DASTZR010000000
VERSION     DASTZR010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773692"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VFV20_07780"
     CDS             <1..494
                     /locus_tag="VFV20_07780"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288286.1"
                     /translation="VPVRPAASAREAVGGADVVVLATNSQDPVIDGAWLEPGQHVGSV
                     QSHELDDATLDHADTIVVRSQDPATFHFPPGRGPIEATEIRELPERLRAKVVTLGDVL
                     LGHGGRGSPSDVTLFTGGGLGASSGLGIQFAATAHAVYRAAIAAGVGRDLPTDWFTET
                     AKP"
     gene            553..1371
                     /locus_tag="VFV20_07785"
     CDS             553..1371
                     /locus_tag="VFV20_07785"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5288287.1"
                     /translation="MGGRDAKLVATDVVVEYWLARSETLFTAVEGVDLQIRDGEFVAI
                     VGPSGCGKTTFLNAVDGLIPIADGSLTLDGREITAPGRDRAMVFQQPGLLPWRTVLGN
                     VMFGVEAQGTMTKSEALDRARQQIDLVGLNGFEDAYPLELSGGMQQRVNLARALLTDP
                     EMLLLDEPFAALDAQTREMMQLELLKIWNKSRKTALFITHDIKEAIYLADRVIVFTKR
                     PGRVKLELEIDIPRPRDLRVKRDPKFVGYEDQIWESIQEELAVKPERLLAGMPG"
     gene            1368..2231
                     /locus_tag="VFV20_07790"
     CDS             1368..2231
                     /locus_tag="VFV20_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5288288.1"
                     /translation="MSIKEVDQVLPVARTEPARPRTRRSAVKPRPWKRYEATAIAAVS
                     VTIVLSTWQMIANARVVPPLFLPGPVEIAQALVKLFNDPRTNIWLDLSTSGQEIAVGY
                     GMAIVLGLVLGILMGWYTRFQYALDPYVNFFYSTPRIVLVPIFIIWFGIGWESKVAVI
                     FLGALFPIIINTMAGVRNTDAALLRVAKSFGASEALVFRRVVLPGAVPFILTGFRLGV
                     GHALTGVVVGELIAAEHGIGKLISDAGVTFQTPKMFAGVVFIAGTGMMLTWLLQRVEN
                     RFQSWRPQIKS"
     gene            2245..>2847
                     /locus_tag="VFV20_07795"
     CDS             2245..>2847
                     /locus_tag="VFV20_07795"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5288289.1"
                     /translation="MRLRLTVIGTVMAILAAACGGTPAATSAPATSQAAATSAASTAA
                     AATKRPCCTKVIAAYSNITADNWVMWYAFENGIFKENGLEVDMKSISGGAQTSAALLA
                     NQVHIAQFGGSEALSANAAGADLVIVGQMAPVYPYILYAKKDIKTIQDLKGKKIGASN
                     KGGSSDIATRAALKANGLDPDKDVSIQYVGSHANRTAALLA"
BASE COUNT          475 a          985 c          965 g          422 t
ORIGIN      
        1 gcgtgccggt ccgcccggcc gcgtcggcac gcgaggcggt gggcggcgcc gacgtcgtcg
       61 tcctcgcgac gaactcccag gacccggtga tcgacggcgc gtggctcgag ccggggcagc
      121 acgtcggctc ggtccagagc cacgagctcg acgacgcgac gctggaccac gccgacacga
      181 tcgtcgtgcg cagccaggat cccgcgacgt tccacttccc gccgggacgc ggccccatcg
      241 aggcgaccga gatccgcgag ctgcccgagc gcctgcgcgc gaaggtggtc acgctcggcg
      301 atgtgctgct tggacatggc ggccgcggct cgccatccga cgtcacgctc ttcaccggcg
      361 gcggcctggg cgcgtcgtcg gggctcggca tccagttcgc cgccaccgcg cacgccgtct
      421 accgcgccgc gatcgccgcg ggcgtcggcc gcgacctgcc gaccgattgg ttcacggaga
      481 cggcgaagcc ctgaccgccc cgcggaaatc gcttgcgcgt gaccctattg gtactaccat
      541 tcgcggcctc agatgggggg ccgagacgcc aagctggtcg cgaccgacgt cgtcgtcgag
      601 tactggctcg cgcgctcgga aacgctcttc acggccgtcg agggtgtgga cctccagatc
      661 cgtgacgggg agttcgtggc catcgtcggc ccgtccggct gcggcaagac gacgttcctc
      721 aacgcggtcg atggcctgat cccgatcgcc gacggctcgc tcacgctcga cggccgcgag
      781 atcacggcgc ccggccgtga ccgggccatg gtcttccagc agcccgggct gctcccctgg
      841 cggacggtgc tcggaaacgt catgttcggc gtcgaagccc agggcacgat gacgaagagc
      901 gaggcgctcg accgggcgcg ccagcagatc gacctcgtcg gcctcaacgg attcgaggac
      961 gcctacccgc tcgagctctc gggcggcatg cagcagcgcg tcaacctcgc gcgcgcgctc
     1021 ctcaccgatc ccgagatgct gctcctcgac gagccgttcg ccgcgctcga cgcgcagacg
     1081 cgcgagatga tgcagctcga gctgctgaag atctggaaca agagccgcaa gaccgccctc
     1141 ttcatcacgc acgacatcaa ggaggcgatc tacctcgccg atcgggtgat cgtgttcacg
     1201 aagcgaccgg gccgcgtgaa gctcgagctc gagatcgata tcccgcgccc gcgcgacctg
     1261 cgggtcaagc gggacccgaa gttcgtgggg tacgaggatc agatctggga gagcatccag
     1321 gaggagctcg cggtgaagcc cgagcgcctg ctggcaggta tgccgggttg agcatcaaag
     1381 aggtcgacca ggtgctgccg gtcgcgcgga ccgagccggc gcgtccacgc acgcgccggt
     1441 cggccgtgaa gccgcgaccc tggaagcgct acgaggcgac cgcgatcgcc gcggtgagcg
     1501 tcacgatcgt cctcagcacc tggcagatga tcgcgaacgc gcgcgtggtg ccgccgctct
     1561 tcctgcccgg accggtcgag atcgcgcagg cgctggtgaa gctcttcaac gatccacgca
     1621 cgaacatctg gctcgatctc tccaccagcg gtcaggagat cgccgtcggc tacggcatgg
     1681 cgatcgtcct cggtctcgtg ctcgggatcc tcatgggctg gtacacgcgc ttccagtacg
     1741 cgctcgatcc gtacgtgaac ttcttctaca gcaccccgcg catcgtgctg gtgccgatct
     1801 tcatcatctg gttcggcatc ggctgggagt cgaaggtcgc ggtcatcttc ctcggcgcgc
     1861 tcttcccgat catcatcaac acgatggccg gcgtccggaa cacggatgcc gcgctgctgc
     1921 gcgtggccaa gtccttcggc gcgtcggagg cactcgtgtt caggcgcgtc gtgctgcccg
     1981 gcgcggtgcc cttcatcctc acggggttcc gtctcggcgt cgggcacgcg ctcaccggcg
     2041 tcgtcgtcgg cgagctcatc gccgccgagc acggcatcgg caagctcatc tccgacgccg
     2101 gcgtgacctt ccagacgcca aagatgttcg ccggcgtcgt cttcatcgcg ggcaccggca
     2161 tgatgctcac gtggctgctc caacgggtcg aaaaccggtt ccagagctgg cggccccaga
     2221 tcaaaagcta ggcgggaggg acacatgcgg ctaagactca cggtcatcgg aacggtcatg
     2281 gcgatcctcg ccgcggcatg cggcggcaca ccggccgcga cgagcgcgcc ggcgacgagc
     2341 caggctgcgg cgacgagcgc cgcgagcacc gcggcggcgg cgacgaagcg cccgtgctgc
     2401 acaaaggtga tcgcggcgta cagcaacatc accgccgaca actgggtgat gtggtacgcg
     2461 ttcgagaacg ggatcttcaa ggagaacggc ctcgaggtcg acatgaagtc gatcagcggc
     2521 ggcgcgcaga cgagcgcggc gctgctggcg aaccaggtgc acatcgcgca gttcggcgga
     2581 tccgaggcac tgagcgcgaa cgcggcgggc gcggacctcg tcatcgtcgg acagatggcg
     2641 cccgtatatc cgtacatcct gtacgcgaag aaggacatca agacgatcca ggacctcaag
     2701 ggcaagaaga tcggcgcgtc caacaagggc ggctcgtccg acatcgcgac acgagcggca
     2761 ctgaaggcga acggcctcga ccccgacaag gacgtctcga tccagtacgt cgggtcgcac
     2821 gcgaaccgca cggcggcgct cctcgcg
//
LOCUS       DASTZR010000348         2686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_881367, whole genome shotgun sequence.
ACCESSION   DASTZR010000348 DASTZR010000000
VERSION     DASTZR010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881367"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..316)
                     /locus_tag="VFV20_07800"
     CDS             complement(92..316)
                     /locus_tag="VFV20_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288290.1"
                     /translation="MSTMWTLIDVCFSDARKAQDLVSDAAARRQLRLRPDAALAEFGL
                     GKPIERTRSTRPILVRVQRRAYATVEAAER"
     gene            434..1366
                     /locus_tag="VFV20_07805"
     CDS             434..1366
                     /locus_tag="VFV20_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019004778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5288291.1"
                     /translation="MIRVEELAKRYKKADRNAVDGVSFEVRAGEFFALLGPNGAGKTT
                     TISILTTTLAPSSGTATIDGRDIVREASAVRRIVGIIFQKPSLDMNLTAEENVRFHAI
                     LYGLYPYAPTFALMPREYRDEMHRLAELLGIREDLHKPMRTFSGGMRRKLEIVRSLIH
                     RPKVLFLDEPTAGLDVPSRRTLWDYLRTIRREAGTTVFLTTHYLEEAEEADHICIIDK
                     GRIVSFGSPTDIKKDLVQEYVLVDADDRRRLREEIGRLELPIEGEGPFKILLSGRGVH
                     PVLKSIDTPLSLVRTHTPTLEDAYLEIVAPKGEE"
     gene            1476..2246
                     /locus_tag="VFV20_07810"
     CDS             1476..2246
                     /locus_tag="VFV20_07810"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5288292.1"
                     /translation="MSELSAVMTLAHRDFVKLLRDRTRIVADFSFPLIFIGILGTSLQ
                     AGFGAAAGIDLMQFVFTGVLAQTVWQSGAMGVISLLADREQDFSQEIFVSPISRYSIV
                     IGKIVGEALVAMPQAIAILAFGWILGVRYSLAMLVALLAVSLLVAVFGGAFGVLVLSR
                     LDNQRQANQVFPFVMLPQFFLAGIFNPIANLPWYLDVLSKLAPMRYAVDLMRDVFYAG
                     GPPVPLADLPTNLGIIAAEFVVFMLVGTAGFVRAERNR"
     gene            2270..2524
                     /locus_tag="VFV20_07815"
     CDS             2270..2524
                     /locus_tag="VFV20_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288293.1"
                     /translation="MPLRRLKKTARTIDAALRDAHKARDAHSDPRFDRSLQDDRRTTL
                     RKFDTVEHALEDREKLERAKAGAKPGPGATNASKAKAKKK"
BASE COUNT          444 a          958 c          859 g          425 t
ORIGIN      
        1 cctcgcggcc aacctgtact cctcgcgcga gtgggaagag ctcgaggacg agcccgtccg
       61 cgagcaccgc cgccgccgcg ccgcccgctg actaccgctc ggcggcctcg accgtggcgt
      121 acgcgcgtcg ctgcactcga acgaggatcg gtcgcgtcga tctcgtccgc tcgatcggct
      181 tcccgagccc gaactccgca agcgcggcgt ccgggcgcag tcgcagctga cgccgcgcgg
      241 cggcgtccga gacgaggtcc tgcgccttcc gtgcatcgct gaagcacacg tcgatgagcg
      301 tccacatcgt cgacatcacc gctacctcca tccgagttcg tgcgagcagc atccgccgga
      361 tgcggacgga cgaccgcgat cgcgcgaacg cgcgctctat caagtccgtc acggtcagcc
      421 ataaacttcc gcgatgatcc gcgtcgaaga gctcgcgaag cgctacaaga aggccgaccg
      481 gaacgccgtc gatggcgttt cgttcgaggt ccgcgccggc gagttcttcg cgctcctcgg
      541 tccgaacggg gcggggaaga cgacgacgat ctccatcctc acgaccacgc tcgcgccatc
      601 ctcggggacc gcgacgatcg atgggcgcga catcgtgcgc gaggcgagcg cggtgcggcg
      661 catcgtcggc atcatcttcc agaagccgag cctcgacatg aatctcaccg ccgaggagaa
      721 cgtgcgcttc cacgcgatcc tctacggcct gtatccgtac gcgccgacgt tcgctctgat
      781 gccgcgcgaa tatcgcgacg aaatgcaccg gctcgcggag ctccttggga tcagggagga
      841 tctccacaag ccgatgcgca cgttctcggg tggcatgcgg cgcaagctcg agatcgtccg
      901 gagcctcatc caccgtccca aggtgctctt tctcgacgag cccaccgcgg ggctcgacgt
      961 gccgagccgc cggacgctgt gggactacct ccggacgatc cgccgcgagg ccggtaccac
     1021 cgtcttcctg accacgcact acctcgaaga ggcggaggag gcggaccaca tctgcatcat
     1081 cgataaggga cgcatcgtct ccttcgggtc gcccacggac atcaagaagg acctcgtcca
     1141 ggagtacgtg ctcgtcgatg ccgacgaccg ccgccggctg cgggaggaga tcgggcgtct
     1201 cgagctcccg atcgagggtg aaggcccctt caagatcctc ctttcgggcc gcggcgtgca
     1261 tcccgtgctg aagtcgatcg acacgccgct ctcgctcgtc cggacgcata cgcccacgct
     1321 cgaggacgcg tacctcgaga tcgtcgcacc aaagggtgag gaatgaagag cacgaagcgg
     1381 cgagcgattc attcgcgagg agcggagctc gacgagcggc gtcgaggcgg cgaggcgcag
     1441 gccgccgcag gcgggccgag ccgagcgcga agaatgtgag cgagctctct gcggtcatga
     1501 ccctggcgca ccgcgacttc gtgaagctcc tgcgcgaccg cacccggatc gtggccgact
     1561 tctcgttccc gctcatcttc atcgggatcc tcggcacgag cctgcaggcc ggtttcggcg
     1621 cggccgccgg catcgacctc atgcagttcg tcttcaccgg cgtgctcgcg cagaccgtct
     1681 ggcagtcggg cgcgatgggc gtgatctcgc tcctcgccga ccgcgagcag gacttcagcc
     1741 aggagatctt cgtctcgccg atctcgcgct actcgatcgt catcggcaag atcgtcggcg
     1801 aggcgctcgt cgcgatgccg caggccatcg cgatcctcgc attcggatgg atcctcggcg
     1861 tgcgctacag cctcgcgatg ctcgtggcgc tgctcgcggt cagcctcctg gtcgccgtgt
     1921 tcggcggcgc cttcggcgtg ctcgtgctct cgcggctcga caaccagcgc caggcgaacc
     1981 aggtcttccc gttcgtgatg ctgccgcagt tcttcctcgc cgggatcttc aaccccatcg
     2041 cgaacctgcc gtggtacctc gacgtgctgt cgaagctcgc gcccatgcgc tacgcggtcg
     2101 acctgatgcg cgatgtgttc tacgccggtg gcccgcccgt cccgctcgcc gacctgccga
     2161 ccaacctcgg catcatcgcg gccgaattcg tcgtcttcat gctcgtgggc acggccggtt
     2221 tcgtccgcgc ggagcgcaat cgctaggcgc ggctgaatac aatcagcgca tgcccctacg
     2281 gcggctgaag aagacggcac ggacgatcga cgcggcattg cgcgacgccc acaaggcgcg
     2341 agacgcgcac agcgatccga gattcgatcg cagtctccag gacgaccgcc ggaccacgct
     2401 gcgcaagttc gacaccgtcg agcacgcgct cgaagatcgc gagaagctcg agcgggcgaa
     2461 ggccggcgcg aagcccggcc ccggagcgac caacgcgtcg aaggcgaagg cgaagaagaa
     2521 atagccgcgc gccccgaggg cgtcgtctgc cgcccgatcc gcgacgacga gttcacattg
     2581 gccgcagacc tcttcttcga cgcgctcgcc gacctcaccg ctcgtcttgg catgccggcg
     2641 ccgcaccgcg accgcgacct ggtcgcgcac ggctacgcct acgtgg
//
LOCUS       DASTZR010000349         2475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1168614, whole genome shotgun sequence.
ACCESSION   DASTZR010000349 DASTZR010000000
VERSION     DASTZR010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1168614"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..755)
                     /locus_tag="VFV20_07820"
     CDS             complement(66..755)
                     /locus_tag="VFV20_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU5288294.1"
                     /translation="MDDAVRAAIARSFLAALPEATLERLLDGAILESVDAGATTYRAG
                     GEPRPSLIVSGLFRSYFAGPDGRQVTVRYAREGDVLGVVGAHGGPAPIHVQALTDASR
                     LRFDVETLRDLAKTDATVAWVLVGELTSTVHALWHELAATAFATVPQRVARHLLEIAA
                     REQEAEGGTLVAHVSQQELADLAGSVREVVARALRELRDEGIVRVSRSGITVLDAAAL
                     ASRSWPRDVGH"
     gene            855..2429
                     /locus_tag="VFV20_07825"
     CDS             855..2429
                     /locus_tag="VFV20_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEU5288295.1"
                     /translation="MTTSFDLAGEAARGNREGMRALVSELRERLATSRLGGPERARQR
                     HLAAGKLLPRDRVERLLDPGSAFLELSPLAAFGMYDDEAPGAGVITGIGRVSGVTCVI
                     VANDATVKGGSYYPLTVKKHLRAQEIALENSLPCIYLVDSGGAFLPRQDDVFPDRDHF
                     GRIFYNQARLSAANIPQLAAVMGSCTAGGAYVPAMSDETVIVKGQGTIFIGGPPLVKA
                     ATGVDVTAEELGGADVHTRVSGVADHLALNDQHAIEIMREIVAQIDRPILWGADRAKP
                     EPPALDPADLYAIVPRDLRTQYDVHEVIARIVDGSRFHEFKARYGTTIVCGFARIEGF
                     RVGIVANNGVLFSESALKAAHFVELCNQRGVPLVFLQNISGFMVGKEYETGGIAKDGA
                     KMVTAVACSVVPKFTVIIGGSFGAGNYGMAGRAYQPRQLWMWPNARISVMGGEQAATV
                     LSMVGTFESDQERERFKTRIREQYESQGSPYYSTARIWDDGVIDPLDTRRVLALGLAA
                     AANGPVPETTFGVFRM"
BASE COUNT          369 a          862 c          887 g          357 t
ORIGIN      
        1 gatcggaatg cgtcgagcag agcgtcctcc tggggccctc agtctcgccg acgtgcaccg
       61 gccggtcagt gacctacgtc acggggccac gagcgcgacg cgagcgcggc cgcgtccagc
      121 acggtgatac cggatcggct gacgcgcacg atgccttcgt cgcgcagctc gcggagcgcg
      181 cgggcgacga cctcgcgcac cgacccagcg agatcggcga gctcctgctg gctcacgtgc
      241 gcgaccagcg tgccgccttc ggcctcctgc tcgcgcgcgg cgatctcgag gagatggcgt
      301 gcgacacgct gtggcacagt cgcgaacgcc gtggccgcga gctcgtgcca cagcgcgtgc
      361 acggtgctgg tgagctcgcc caccaggacc cacgcgacgg tcgcgtccgt cttcgcgagg
      421 tcgcgcagcg tctcgacgtc gaagcgcagc cggctcgcgt cggtgagcgc ctgcacgtgg
      481 ataggcgccg gaccgccgtg cgcgccgacg acgccgagca cgtcgccctc gcgcgcgtag
      541 cggaccgtga cctgacgtcc atccggaccg gcgaagtacg agcggaacag gccgctgacg
      601 atcagcgagg gccgcggctc gccaccggcg cgataggtcg tggcccccgc gtcgaccgac
      661 tcgaggatcg cgccgtccag gagccgctcc agcgtcgcct ccggcaatgc ggcgaggaag
      721 ctgcgcgcga tcgcggcgcg gacggcgtcg tccatcgttc ccgatggtag gtcgatgagc
      781 gttcgtacca gcgtcggcgc ggcggtctcg ggcagggccc ggcctctcga cctagcatcg
      841 gggccgtgac ggtcatgacg acctccttcg atctcgccgg ggaggccgcc cgcggcaacc
      901 gcgaggggat gcgcgcgctc gtcagcgagc tgcgcgaacg actcgcgacg tcacgtctcg
      961 gcggtccgga gcgcgcacga cagcgccatc tcgccgcggg caagctgctg ccgcgcgacc
     1021 gcgtcgagcg gctgctcgac ccgggcagcg cgttcctgga gctctcgccg ctggcggcct
     1081 tcgggatgta cgacgacgag gcgccgggcg cgggtgtgat cacgggcatc ggacgcgtga
     1141 gcggcgtcac gtgcgtcatc gtcgcgaacg acgccaccgt gaagggcggc tcgtattacc
     1201 cgctgacggt caagaagcat ctgcgcgcgc aggagatcgc gctcgagaac agcctgccgt
     1261 gcatctacct ggtcgactcg ggcggcgcct tcctgccacg ccaggacgac gtcttcccgg
     1321 atcgcgacca tttcggccgc atcttctaca accaggcgcg gctctccgcg gcgaacatcc
     1381 cgcagctcgc ggccgtcatg ggctcgtgca cggccggcgg ggcgtacgtg ccggcgatga
     1441 gcgacgagac cgtgatcgtc aagggtcagg gcacgatctt catcgggggc ccgccgctgg
     1501 tgaaggcggc gaccggcgtc gacgtgaccg cggaggagct gggcggcgcg gacgtgcaca
     1561 cgcgggtctc cggcgtcgcc gatcatctcg cgctgaacga tcagcacgcg atcgagatca
     1621 tgcgtgagat cgtcgcgcag atcgaccgcc cgatcctgtg gggcgctgat cgggcgaagc
     1681 cagagccgcc cgcgctcgat cccgccgacc tctacgcgat cgtgccccgc gacctgcgca
     1741 cgcagtacga cgtccacgag gtcatcgcgc gcatcgtgga cggatcgcgc ttccacgagt
     1801 tcaaggcgcg ctacggcacg accatcgtgt gcggcttcgc ccgcatcgag ggttttcgcg
     1861 tggggatcgt cgcgaacaac ggcgtgctgt tcagcgagtc cgccctgaag gccgcgcatt
     1921 tcgtcgagct ctgcaaccag cgcggggtgc cgctcgtctt ccttcagaac atctccggct
     1981 tcatggtcgg caaggagtac gagaccggcg gcatcgccaa ggacggagcg aagatggtca
     2041 ccgccgtggc gtgcagcgtc gtgcccaagt tcaccgtcat catcggcggc tcgttcggcg
     2101 ccgggaacta cggcatggcc ggccgcgcgt accagccgcg gcagctgtgg atgtggccga
     2161 acgcccgcat cagcgtcatg ggtggtgagc aggccgcgac cgtcctctcg atggtcggca
     2221 cgttcgagag cgaccaggaa cgcgagcgct tcaagacgcg tatccgcgag cagtacgagt
     2281 cgcagggctc gccgtactac tcgaccgcgc gcatctggga cgacggggtc atcgatccgc
     2341 tggacacgcg ccgcgtgctc gcgctcggcc tggccgcggc ggcgaacggg cccgtgccgg
     2401 agacgacgtt cggcgtgttc cggatgtgag ggcgacccga cgtctctcgc gagggtgacg
     2461 cgatggacgc cgagc
//
LOCUS       DASTZR010000350         2089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_281211, whole genome shotgun sequence.
ACCESSION   DASTZR010000350 DASTZR010000000
VERSION     DASTZR010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281211"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VFV20_07830"
     CDS             complement(<1..244)
                     /locus_tag="VFV20_07830"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU5288296.1"
                     /translation="MGDLAEAMGLQKGSLYSLTESKQELLYETMREGAAAFHAGLDAL
                     PEDAGAAERIRLALRAHLRVVAEQLDMATVFIREWRY"
     gene            357..1256
                     /gene="paaA"
                     /locus_tag="VFV20_07835"
     CDS             357..1256
                     /gene="paaA"
                     /locus_tag="VFV20_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HEU5288297.1"
                     /translation="MTAREQEFLEYVQSGGLVETDDWMPDEYRARLIKFIEMHGNSEL
                     MGVLPERDWILRAPTLQRKLALTAKIQDEVGHCQLIYRVVEDLGKPREQCLDDLIAGK
                     SKFHNVFHYPTKSWGDVGVIAWLVDAAAIISQKALLKCSYAPYARVMKKVCWEESFHI
                     LHGRDTVLAMVTGTPEQFELVQEALNRWWGPLMHFHGTPIPAEEDPMVIWRIKSQGNE
                     DARQQFLDGYVPQIRELGLELPDPKLRKREDGVWEYTEPDWDELLSVVTGHGPASQER
                     LAFRRMSRAEVDWVAKVVLAEAA"
     gene            1253..1573
                     /locus_tag="VFV20_07840"
     CDS             1253..1573
                     /locus_tag="VFV20_07840"
                     /inference="COORDINATES: protein motif:HMM:NF018002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaB"
                     /protein_id="HEU5288298.1"
                     /translation="MIWEVFRQERKGQPFVHGGSFHAPDQAFADAYAREFYARRQESV
                     ALWVVPREAIHAIEEFPDEFDLKYRRVDGYSIKARLKEARARVGSTERANAEVPALPA
                     PERT"
     gene            1570..>2089
                     /gene="paaC"
                     /locus_tag="VFV20_07845"
     CDS             1570..>2089
                     /gene="paaC"
                     /locus_tag="VFV20_07845"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEU5288299.1"
                     /translation="MSSARAPSDLLLQLADDELILGWRNSEWTGIAPFLEEDVAFSSI
                     AQNEIGHARALYELAARELGGDADAIAFDRKPEEYRCAPLVQLQLVPDWARTIARHVL
                     YETADAIRIERLKSHGDAELAGIAAKIEREEVYHRLHAEMWLERLRDEPRYADAVAEL
                     WPYALGVLDPELR"
BASE COUNT          332 a          714 c          752 g          291 t
ORIGIN      
        1 ggtaccgcca ctcgcggatg aagacggtcg ccatgtcgag ttgctccgcg acgacgcgca
       61 ggtgcgcccg cagcgcgagc cggatccgct cggccgcgcc ggcgtcctcg ggcagcgcgt
      121 cgaggcccgc gtggaaggcc gcggccccct cccgcatcgt ctcgtacagc agctcctgct
      181 tcgactcggt gagcgagtag agcgagccct tctgcagccc catcgcctcg gcgaggtcgc
      241 ccatcgacgt gccgtgatag cccttctccg cgaagagccg ggcggcctga cgggtcagct
      301 cctggcggcg agtggcgata cactcaatgc taacgaccgt tcggcaggga ggcaggatga
      361 cagcgcgcga gcaggagttc ctcgagtacg tccagtccgg cgggctcgtc gagacggacg
      421 actggatgcc ggacgagtac cgcgcccggc tgatcaagtt catcgagatg cacggcaact
      481 ccgagctgat gggcgtgctc cccgagcgcg actggatcct gcgcgcaccc acgctgcagc
      541 gcaagctcgc gctgaccgcg aagatccagg acgaggtcgg ccactgccag ctgatctacc
      601 gcgtcgtcga ggacctcggc aagccgcgcg agcagtgcct cgacgacctg atcgccggca
      661 agtcgaagtt ccacaacgtc ttccactacc cgacgaagag ctggggcgac gtgggcgtga
      721 tcgcctggct cgtcgatgcc gccgcgatca tcagccagaa ggcgctgctg aagtgctcgt
      781 acgcgccgta cgcccgcgtg atgaagaagg tctgctggga ggagtcgttc cacatcctcc
      841 acggccgcga cacggtgctc gcgatggtga ccggcacgcc ggagcagttc gagctcgtgc
      901 aggaggcgct gaaccgctgg tgggggccgc tgatgcactt ccacggcacg ccgatccccg
      961 ccgaagagga cccgatggtg atctggcgga tcaagagcca gggcaacgag gacgcgcggc
     1021 agcagttcct cgacggctac gtgccgcaga tccgcgagct ggggctcgag ctccccgacc
     1081 cgaagctgcg caagcgcgaa gacggcgtct gggagtacac cgagcccgac tgggacgagc
     1141 tgctctcggt cgtcaccggc cacgggcccg cgtcgcagga gcggctcgcg ttccggcgca
     1201 tgtcccgcgc ggaggtggac tgggtcgcga aggtcgtcct cgcggaggcg gcgtgatctg
     1261 ggaggtcttt cggcaggagc ggaagggcca gccgttcgtg cacgggggct cgttccacgc
     1321 gcccgaccag gcgttcgccg acgcgtacgc gcgcgagttc tacgcgcggc ggcaggagtc
     1381 ggtggcgctc tgggtcgtgc cgcgcgaggc gatccacgcg atcgaggagt tcccggacga
     1441 gttcgacctc aagtaccggc gcgtcgacgg ctactcgatc aaggcgcggc tgaaggaggc
     1501 gcgtgcgcgc gtaggctcga cggagcgcgc gaacgcggag gtgccggctt tgccggcacc
     1561 ggagcgaaca tgagcagcgc gcgggcaccg tctgacctgc tgctccagct cgccgacgac
     1621 gagctgatcc tcggctggcg caactccgag tggacgggga tcgcgccgtt cctcgaggag
     1681 gacgtcgcct tctcgtcgat cgcgcagaac gagatcggcc acgcgcgcgc gctctacgag
     1741 ctggccgcgc gcgagctcgg cggcgacgcc gacgcgatcg cgttcgaccg caagcccgag
     1801 gagtaccgct gcgccccgct cgtccagctc cagctcgtgc ccgactgggc gcgcacgatc
     1861 gcgcgccacg tgctctacga gaccgccgac gcgatccgga tcgagcggct gaagtcgcac
     1921 ggggacgccg agctcgccgg gatcgcggcg aagatcgagc gtgaggaggt ctaccaccgc
     1981 ctgcacgccg agatgtggct cgagcggctg cgcgacgagc cgcgctacgc ggacgcggtc
     2041 gcggagctct ggccctacgc gctcggcgtg ctcgacccgg agctgcgcc
//
LOCUS       DASTZR010000351         2392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_515735, whole genome shotgun sequence.
ACCESSION   DASTZR010000351 DASTZR010000000
VERSION     DASTZR010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515735"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..157
                     /locus_tag="VFV20_07850"
     CDS             8..157
                     /locus_tag="VFV20_07850"
                     /inference="COORDINATES: protein motif:HMM:NF024983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEU5288300.1"
                     /translation="MKGRKVHVRYFAVRSADGRYLGTLETVQDIAPLQKIEGERRLLD
                     EALPV"
     gene            242..1903
                     /locus_tag="VFV20_07855"
     CDS             242..1903
                     /locus_tag="VFV20_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687565.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEU5288301.1"
                     /translation="MAPHPKGTRARVLLTSVFKPFAQDDEFGSREQNPIELYHNQVTR
                     EQGPFSLRMFHRSWGLMFLQQNITAPCTLLDFPSREEFVAELSAHAYDIVGISSIIVN
                     VGKVKEMCRLVRQHSPHSTIVVGGHVAAIPGIERMIDADHVVKGEGIAWLREFLGEKV
                     DRPIVHPIIPSSFDLRVMGMPNLQRKPTSATIVSSVGCPMGCNFCTTSAFFGGKGNII
                     NFYETGAELFEVMCNAERALNVSSFFMMDENFLLQRTRALELLELMKAAGKSWSLYVF
                     SSANAVKKYKMRELVELGIEWIWLGLESPQSSYAKLNGTDTQALARELQSHGIALLGS
                     TIIGLEHHTPGNIHAEIEHAVAHDAVFHQFMLYTPVPGTPLYKEVEAQQRLRTDVELA
                     DIHGQHEFNFLHGAIGAADSKKFLDYAFRRDFERNGPSLYRFVRTMFERWKKYGASAD
                     PRVRERALRGGRQIKAGYGAVLWAMEKYFRKSNAEVSSRIREVRRDMEREMGGLTTIV
                     TRSLGPLLLWTSRRDARRHPKGRRLEPRTFIERARWPGQAVNKPA"
     gene            complement(1953..>2392)
                     /locus_tag="VFV20_07860"
     CDS             complement(1953..>2392)
                     /locus_tag="VFV20_07860"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEU5288302.1"
                     /translation="GVVGGVAGGLAAQRVGAVAESVRVTAPGEFSSPDGSVAWRVGPA
                     GRLLRSADGGTTWSPQNTGVKIDLLAGSAPSREVCWVVGRAGIVLLSVAGGEWRRIAF
                     PEAVDLVAVSATDAITASVTTVDGRLFATSDGGKIWKMENGKW"
BASE COUNT          429 a          811 c          762 g          390 t
ORIGIN      
        1 gctcgacatg aaggggcgca aggtccacgt tcgatacttc gcggtccggt cggctgacgg
       61 ccgctatctc ggcaccctcg agaccgtgca ggacatcgcg ccgctccaga agatcgaagg
      121 cgagcggcgc ctgctcgacg aagcgctccc ggtctaggtg ccggaatccc ggcagcgtaa
      181 tctttctgtc acatctctcg cgcggcgttc cacgcgggcg gccagtgcct atgctggacg
      241 catggcacca catcccaagg gcacacgcgc gcgcgtcctc ctgacgtccg tcttcaagcc
      301 gttcgcgcag gacgacgagt tcgggtcgcg cgagcagaat ccgatcgagc tgtatcacaa
      361 ccaggtgacg cgcgagcagg gacccttctc gctgcgcatg ttccatcgat cgtggggcct
      421 gatgttcctg cagcagaaca tcacggcgcc gtgcaccctg ctcgacttcc cgtcgcgcga
      481 ggagttcgtc gccgagctct ccgcccacgc ctacgacatc gtcggcatct cgagcatcat
      541 cgtgaacgtc gggaaggtga aggagatgtg ccggctcgtg cggcagcact cgcctcactc
      601 gacgatcgtc gtcggcggcc acgtcgcggc gatccccggg atcgagcgga tgatcgacgc
      661 cgaccacgtc gtgaaggggg aaggcatcgc gtggctgcgg gagttcctcg gcgagaaggt
      721 ggatcggccg atcgtccatc ccatcatccc ctcgtcgttc gacctgcgcg tgatgggcat
      781 gccgaacctc cagcggaagc cgacctccgc gacgatcgtg tcgtcggtcg gctgcccgat
      841 gggctgcaac ttctgcacga cgtccgcgtt cttcggcggc aaggggaaca tcatcaactt
      901 ctacgagacc ggcgccgagc tgttcgaggt gatgtgcaac gcggagcgcg cgctcaacgt
      961 gtcgtcgttc ttcatgatgg acgagaactt cctcctgcag cgcacgcgcg cgctcgagct
     1021 gctcgagttg atgaaggccg cgggaaaatc ctggtcgctc tacgtgttct cgtcggcgaa
     1081 cgcggtgaag aagtacaaga tgcgcgagct cgtcgagctg ggcatcgagt ggatctggct
     1141 cggcctcgag tccccccaga gcagctacgc gaaactgaac ggcaccgaca cgcaggcgct
     1201 cgcccgcgag ctgcagtcgc acggcatcgc gctcctcgga tcgacgatca tcgggctcga
     1261 gcaccacacg ccgggaaaca tccacgccga gatcgagcac gcggtcgccc acgacgcggt
     1321 gttccaccag ttcatgctgt acacgcccgt ccccgggacg ccgctgtaca aggaagtcga
     1381 ggcgcagcag cgcctccgca ccgacgtgga gctcgcggac attcacggac agcacgagtt
     1441 caatttcctg cacggggcga tcggcgcggc cgactccaag aagttcctgg actatgcgtt
     1501 ccgccgcgac ttcgagcgca acggccccag cctctaccgg ttcgtccgca cgatgttcga
     1561 gcggtggaag aagtacggcg cgtcggcgga tcctcgcgtt cgcgaacgtg cgctgcgggg
     1621 aggacgtcag atcaaggcgg gctacggcgc ggtgctctgg gcgatggaaa agtacttccg
     1681 caagtcgaac gcggaggtga gctcccgtat ccgcgaggtc cggcgcgaca tggagcgcga
     1741 gatgggcggc ttgacgacga tcgtcacccg gtcactgggc cccctgctct tgtggacgtc
     1801 gcgccgcgat gcgcgccggc atccgaaggg gcgccgcctc gagccccgca cgttcatcga
     1861 acgcgcccgc tggcctggac aggccgtgaa caaaccggcc tgatggaaaa tggaagatgg
     1921 aaaatggaaa agggacggat ctgaccgcct ctttaccatt ttccattttc cattttccat
     1981 attttgccgc cgtccgaggt ggcgaagagg cggccgtcca ccgtggtgac cgacgcagta
     2041 atcgcgtccg ttgccgagac ggccacgaga tcgacggcct cggggaacgc gatccgcctc
     2101 cactctcctc ctgcgaccga cagcaggaca atccccgcac ggccgacgac ccagcagact
     2161 tcgcgcgagg gcgccgagcc cgccaacaga tcaatcttta cccccgtatt ctgcggcgac
     2221 cacgtcgtgc ccccatctgc cgaacgcagg aggcgtccgg ccggaccgac gcgccaggcc
     2281 accgatccat cgggtgagga gaactcaccg ggtgcggtga cgcgcaccga ttcggcgacc
     2341 gcccccacgc gttgggccgc gagtcctccc gcgactccgc cgaccacgcc ac
//
LOCUS       DASTZR010000352         2434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_227657, whole genome shotgun sequence.
ACCESSION   DASTZR010000352 DASTZR010000000
VERSION     DASTZR010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227657"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..330
                     /locus_tag="VFV20_07865"
     CDS             28..330
                     /locus_tag="VFV20_07865"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEU5288303.1"
                     /translation="MLVARRETSGEEVDAQPIRAPAVLRPSLEPAQAGLMPDVRGLSA
                     REALRTLSRAGVAASLDGSGFVVDQSIPPGAALARGESCILRLGRRAVATIGESTR"
     gene            327..1865
                     /locus_tag="VFV20_07870"
     CDS             327..1865
                     /locus_tag="VFV20_07870"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU5288304.1"
                     /translation="MTLRKLLAAVGDRPPFERRHVDAAGASANATVTAIAYDSRQTTS
                     GAVFVALRGLKADGTTFARDAAARGALAVVSEAPAPEGVSAPWIQVGDGRLALAALAS
                     AFYGDPSRDLLLVGITGTNGKTTTSYVLASIFEAAGMVCGRIGTVGYRIGDREIDAAR
                     TTPEAPELQRMLREMVAQGCRACVMEVSSHALVMRRADYLHFSAAIFTNLTRDHLDFH
                     RDMEEYFLAKRRLFELLPESAFAIVNLDDRRGAELTRVGGRPVTYAVDAAADVRPGPL
                     AFSLDGLSFEVRTPRGSLQIRSSLVGRPNAYNILAAVATAVALDVPFSAIEQGIARLG
                     SVPGRFQVVSGPFDDVRVVVDYAHTDDALKNLLETARPLASGRLVTVFGCGGDRDRTK
                     RPLMGAVAARLSDLVVVTSDNPRSEDPDQIIEQIKKGIVMPADRVPPRGQAPRSTACL
                     AIPDRKEAIERAVRDARPGDLVLIAGKGHERYQEIGGRVLPFDDVDVARAALARRRSA
                     QRAS"
     gene            1947..>2434
                     /gene="murF"
                     /locus_tag="VFV20_07875"
     CDS             1947..>2434
                     /gene="murF"
                     /locus_tag="VFV20_07875"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF013396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEU5288305.1"
                     /translation="MLGRVWIDSRTIQPGDLFVAIRGERFDGHAYAVDALAHGAAGAV
                     VETVPAGGEAAPGSGPVVIQVEDTTRALQDLARSVRRRSGAKVVAITGSAGKTTTKEV
                     TADFLSSRYRVFRNKGNLNNHIGLPLSLIELRDQPDVAVVELGMNHPGEIRTLVGIAE
                     PEV"
BASE COUNT          338 a          836 c          902 g          358 t
ORIGIN      
        1 ggcccgacgc tgaatccgcc gccgccggtg ctcgtcgcga ggcgcgagac gtcgggcgaa
       61 gaggttgacg cgcagccgat ccgagcgccc gccgtgctca ggccgtcgct cgaacccgcg
      121 caggccgggc tgatgcctga cgtgcgcggg ctcagcgcgc gcgaggcgct gcggaccttg
      181 tcgcgcgcgg gtgtcgccgc gtctctcgac gggagcgggt tcgtcgtcga tcagtcgatc
      241 ccgccgggcg cggcgctggc gcgcggtgaa agctgcatcc tgaggctcgg acgccgcgcc
      301 gtcgccacga taggggagag cacgcggtga ccctgcgcaa gctcctcgcc gccgttggcg
      361 atcgtccgcc gttcgaacgg cgccacgtgg acgcggcggg agcctccgcg aatgcgacgg
      421 tcaccgccat cgcgtacgac tcgcgtcaga cgacctcggg cgcggtgttc gtcgccctcc
      481 gtggcctgaa ggcggatgga acgacgttcg cccgcgacgc cgccgcgcgc ggtgccctgg
      541 cggtcgtctc cgaggcgccc gcacccgagg gggtctccgc tccgtggatt caggtcggcg
      601 acggccgcct ggcgctcgcc gcgctcgcct cggcgtttta cggcgacccg agccgcgacc
      661 tgctgctcgt ggggatcacg ggcacgaacg gaaagacgac gacgtcctat gtgctcgcgt
      721 ccatcttcga agcggcgggg atggtatgcg gccggatcgg gacggtcggc taccgcatcg
      781 gcgaccggga gatcgacgcg gcccgcacga cgccggaagc gccagagctc cagcggatgc
      841 tgcgcgagat ggtggcccag ggctgccgcg catgcgtgat ggaagtgtcg tcgcacgcgc
      901 tcgtgatgcg ccgcgccgac tacctccact tctcggccgc catcttcacg aacctcacgc
      961 gcgatcatct cgacttccat cgcgacatgg aggagtactt cctcgcgaag cggcggctgt
     1021 tcgagctgct cccggagtcc gcgtttgcca tcgtgaatct cgacgaccgc cgcggcgcgg
     1081 agctgacgcg cgtcggcggc cgcccggtca cctacgccgt cgatgccgcg gcagacgtgc
     1141 gccccggccc gcttgccttc tcgctcgacg gtctcagctt cgaggtccgc acgccgcgcg
     1201 ggtccctcca gattcgctcg tcgctcgtcg gccgtcccaa cgcctacaac atcctggcgg
     1261 ccgtcgcgac cgcggtggcg ctcgacgtcc cgttctccgc gatcgaacag gggatcgcgc
     1321 gcctcgggag cgtcccgggg cgattccagg tggtgtccgg gccgttcgac gacgtgcgcg
     1381 tggtcgtcga ttacgcgcac accgacgacg cgctgaagaa cctgctggag accgcgcgcc
     1441 cgctcgcgtc cgggcgtctg gtcacggtgt tcggctgcgg cggcgatcgc gatcgcacga
     1501 agcggccgct gatgggcgcg gtcgccgcgc gcctgagcga tctcgtcgtc gtgacgtccg
     1561 acaacccgcg gtcggaggac ccggatcaaa tcatcgagca gatcaagaag ggcatcgtga
     1621 tgccggccga tcgcgtcccg ccgcggggcc aggcgcctcg ctcgacggcg tgcctcgcga
     1681 ttcccgaccg caaggaagcc atcgagcggg cggtgcgcga cgcgcggccg ggcgatctcg
     1741 tgctcatcgc cgggaagggg cacgagcggt accaggagat cggcggccgc gtgctgccgt
     1801 tcgacgatgt ggacgtcgcg cgtgcggcgc tggcgcggcg ccggtcggcg cagagggcct
     1861 cgtagcgagt ggacgggctt ccgctcaccg ccggtgatgt cgccgccgcg accggcggac
     1921 agctgattgg cgggaacgcg caccaggtgc tggggcgcgt ctggatcgat tcgcggacga
     1981 tccagccggg cgacctgttc gtggcgattc gcggcgaacg gttcgacggc catgcgtacg
     2041 ccgtcgatgc gctggcgcac ggcgcggccg gcgcggtcgt ggagacggtg ccggcggggg
     2101 gagaggcggc gcccggatcg gggccggtgg tgatccaggt cgaggacacg acgcgtgcgc
     2161 tacaggacct cgcgcgcagc gtgcggcggc gatcgggggc aaaggtcgtc gcgattacgg
     2221 gcagcgcggg caagaccacg acgaaagagg tcaccgcgga cttcttgtca tcgcggtacc
     2281 gcgtattcag gaacaagggc aacctgaaca accacatcgg cctgccgctg tcgctcatcg
     2341 agctgcggga ccagccggat gtcgcggtcg ttgaactggg catgaaccat ccaggtgaaa
     2401 tccgaacgct tgtcgggatc gcggagccgg aggt
//
LOCUS       DASTZR010000353         6649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1194767, whole genome shotgun sequence.
ACCESSION   DASTZR010000353 DASTZR010000000
VERSION     DASTZR010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6649
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194767"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="VFV20_07880"
     CDS             <1..1050
                     /locus_tag="VFV20_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU5288306.1"
                     /translation="LRVRCLATPGHTPEHVSFLVADRRRDDDAQFLFSGGALLVGHIA
                     RVDLLGPELGERLARDAYATLRDRLLALADYVAVFPTHGGGSACTASSVSGSRWTTLG
                     FERRHNDIARAAMADFEEFRTAISRGLPVAPAYYPHVRALNAQGAPPRTAAPLAVLRD
                     PLPRDAVLLDPRPPHVFAAAHRRGALNAVGNDSFAVRVGAVVPFGAPLVLLGTDPDAA
                     EQLRRQLSTIGYDDVRGMAEPVPESEDMLARSRLVDVRTAQRLMRDGAQVLDVRERSE
                     YEAGHVPHARHIPYEELERRLDELTREQQVVVYCASGVRSSLAASLLEQRGFDSANIR
                     GGLNAWVGADLPIER"
     gene            1168..2364
                     /locus_tag="VFV20_07885"
     CDS             1168..2364
                     /locus_tag="VFV20_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5288307.1"
                     /translation="MRIAFVASAVPRRCGIATFTSDLLNAVKSADPTVRVSQAAIDEP
                     NAARAYGPEVRWRLRQEDWRTYRDAALAINATNIDVVNIQHEFGLYGLWHDGIYDDHL
                     AVFLEHLRKPSVVTLHTVPPRPEPWLRDAVRVASERSDVIVVMAHTAARLLAEVYGIR
                     KEPVVIEHGMPAIEPRGRRRLKRQLGVEDRQIVSTFGLVDPRKGLEYVIDAMPEIVAA
                     SPSALYLIVGQTHPELLKKEGEAYRNSLVATAQRAGVSDHVKFVNEYLTQRQIVDYLL
                     ATDVYVTPYLDPNQITSGTLAYALGAGKAIVSTPYLHAREVLDDGRGVLVPFRDAGAI
                     GHAVTRILGDAALKQRLEARSYEYGKEMAWPAIGRRVLALMRDVVDARADAAHEPVAA
                     GHTRIA"
     gene            2361..3572
                     /locus_tag="VFV20_07890"
     CDS             2361..3572
                     /locus_tag="VFV20_07890"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HEU5288308.1"
                     /translation="MNLGRRLTENPILTAADVPPSLPTLEVVSVFNAAAARVGDEIIL
                     LLRVAERPRTDVAPGPDALTLDFDQPHPVLHPLPARYRDQELVGIAFLDARQSPAKVV
                     VAYIPTDLPGLDLRDPRSIRYRNNTGYLRMTNEGYIDFLAQISHLRVARSRDGVHFTV
                     DAEPAIAPHTEMEEYGVEDPRATLIDGVWHITYVSVSRWGITTSLATTLDFRTFDRRG
                     VIFLPDHKDVVIFPERVGGKYVALTRPMPQSFSRIFGIWMAFSDDLEHWGEHETLALP
                     RWGYWDELRTGASAVPFKTDHGWLELYHGVDRNTTYAMGALLLDPNDPRRIIARSPTP
                     ILEPTEAFERMGLFNDTVFSCGHVDMGDGRIRLYYGAADSVMAAADFEIDEIVRSLEP
                     WHGAAERFALV"
     gene            3572..4672
                     /locus_tag="VFV20_07895"
     CDS             3572..4672
                     /locus_tag="VFV20_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288309.1"
                     /translation="MLRQLARLSVAVPAAGDRATAIAVYGDSTDRALVAADLGHEGVA
                     CVDDVARAAVLLCDLWESTRLPRARAWALSLVDFLLYMQLGDGRFVNFIVDWDGRHND
                     RGPTSFAGGGFWHARGVRALAKAWLALGDERARQGLALGLPLIREGRDVPADVRSIHA
                     LMAVELVRSGQLVELATDLERWCAEIVALRGGDILLDNPDEAEPHLWGHLQEGVLAEA
                     GRLLDRPDLIEIARRSAVAYLAPLIESGFDLPMVQPYGVASAAYGVERLAAATHDPAF
                     RGLAAKARAWFGGRNSAHLPVYDSGSGRVHDGIDDGVLNPHSGAESNIVAAQALIADL
                     VRIAPALFPVVDACFPRTVRERIAGGALQRTA"
     gene            4710..5636
                     /locus_tag="VFV20_07900"
     CDS             4710..5636
                     /locus_tag="VFV20_07900"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEU5288310.1"
                     /translation="MRRLLAIIVAALALAAAVAMSCGLLPSDPRAAGTKVPQGAPLPL
                     AIEEDPGDPEPDLNAPPVALAPPGGAIIQQGVVRIPKPKIDVNGKRRVGIQIGHWQTD
                     TVPKEYGTRIQQQTGTSWEGITEVEVTTEIANRLAALLEAQGIAVDILPTTVPEGYLA
                     DVFIALHVDGDGVGELSGFKMAHGTRRGPFEDQLMRTIKDVYAKATGLRYDTEHVTRA
                     MTGYYAFNWGRYMHATSPFTPSTILELGFLSNDDDRALLLDQTDTIAAALSTGILTFL
                     DETPRSKLFGSDLLVPQAPIRQGPLPTPTTPP"
     gene            complement(5637..6254)
                     /locus_tag="VFV20_07905"
     CDS             complement(5637..6254)
                     /locus_tag="VFV20_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288311.1"
                     /translation="MGRIPELLGRAGTLARQPAGLVNIASRTAIFAFTIDALVNAGDE
                     RFAGKALGPRNVGILLGLSMLFPILHWIYHRWERYPLWYDDLYLSIFWLDMSGNSLDL
                     YNTVEWWDHVPHFHGPGALAMVLVGAFGLQALAAVGFSTMLHVLLEVQEYYGDVLLGT
                     HNVKGIADSINDLAYGLAGVILYGLVAKRWMYLRRRRTRPTKRRG"
BASE COUNT         1006 a         2384 c         2267 g          992 t
ORIGIN      
        1 ctgcgcgtcc ggtgtcttgc gacgcccggg cacacgcccg agcacgtgtc ctttctcgtc
       61 gcggaccggc gtcgcgacga cgacgcgcag ttcctcttca gcggcggcgc gctcctggtc
      121 ggccacatcg cgcgcgtcga cctgctcggg cccgagctcg gcgagcggct cgcgcgagac
      181 gcgtacgcca cgctgcgaga tcgtctgctc gcgctcgcgg attacgtcgc ggtcttcccg
      241 acgcacgggg gcggaagcgc ctgcaccgcc tcatcggtgt cgggctcacg ctggacgacg
      301 ctcggcttcg agcggcgcca caacgacatt gctcgcgccg ccatggcgga cttcgaggaa
      361 ttccgtacgg cgatctcacg gggtctcccg gtcgctccgg cgtactaccc gcacgtccgc
      421 gcgctgaacg cgcagggagc gccgccacgg acggcggcgc cgctcgcggt cctccgcgac
      481 ccgcttccgc gtgatgccgt gctcctcgac ccgcgtccac cgcatgtgtt cgcggccgcg
      541 caccgccgtg gcgcgctgaa cgccgtcggc aacgactcgt tcgccgtgcg cgtcggcgcc
      601 gtcgtgccgt tcggcgcacc actcgtgctg ctcggcaccg accccgatgc cgcggagcag
      661 cttcgccgcc agctctcgac gatcgggtac gacgatgtgc gcggcatggc ggagcccgtg
      721 cccgagtccg aggacatgct cgcgcggtcg cgactcgtcg atgtccgcac cgcgcagcgc
      781 ctgatgaggg atggtgcgca ggtcctggat gtccgagaac gctcggaata cgaggccggt
      841 cacgttccgc acgcacgtca catcccgtac gaggagctcg aacgtcgtct cgacgagctg
      901 acgcgcgagc agcaggtcgt cgtgtactgc gccagtggtg tacgatcaag cttggccgcc
      961 agcctccttg agcagcgcgg cttcgattcc gccaacatcc gcggcggtct caatgcctgg
     1021 gtaggagccg acctgccgat cgagcgctga ccgtcctttc ggcacgcgtc ctgcggcgaa
     1081 aggggtctgc cgatgtcgtc actgccgaac gggcagcacg cccgtgtcct gacgaaggac
     1141 cgagcgctga ttggggaaga tggtcatatg cggatcgcct tcgtggcctc cgcggtgccg
     1201 cggcgctgcg ggatcgccac attcacatcc gacctgctga acgcggtcaa atccgcggat
     1261 ccgaccgtgc gcgtgtccca ggccgccatc gacgagccga acgccgcgcg tgcctacgga
     1321 cctgaggtcc gctggcgcct gcggcaggag gattggcgca cctatcgcga cgcggcgctc
     1381 gcgatcaacg ccacgaacat cgacgtggtc aacatccagc acgagttcgg gctgtatggg
     1441 ctctggcacg acggcatata cgacgatcac ctcgccgtct tcctcgagca cctgcgcaag
     1501 ccatcggtgg tcacgctgca tacggtgccg ccgcgccccg agccgtggct gcgtgacgcg
     1561 gtgcgtgtcg catccgagcg cagcgacgtc atcgtcgtca tggcgcacac tgcggcgcga
     1621 ctgctggccg aggtctacgg catccggaag gagccggtcg tcatcgagca cggcatgccg
     1681 gcgatcgagc cccgcggccg ccgccgcctg aagcggcagc tcggcgtcga ggatcggcag
     1741 atcgtctcga ccttcgggct cgtcgatccc cggaagggac tggagtacgt catcgacgcg
     1801 atgcccgaga tcgtggccgc gtctccgagc gcgctgtacc tcatcgtcgg gcagacgcat
     1861 cccgagctcc tgaagaagga gggcgaggcc tatcgcaact ccctcgtcgc gacggcccag
     1921 cgcgccggcg tgagcgacca cgtgaagttc gtgaacgagt acctcacgca gcgccagatc
     1981 gtcgactacc tcctcgccac cgacgtgtac gtcacgccgt atctcgaccc gaaccagatc
     2041 accagcggca ccctcgcgta cgcgctggga gccggaaagg cgatcgtgtc cacgccctac
     2101 ctgcacgcgc gcgaggtcct cgacgacggc cgcggcgtgc tcgtgccgtt ccgtgacgcc
     2161 ggcgccatcg ggcacgcggt cacgcgcatc ctcggcgacg cggcgctcaa gcagcgtctc
     2221 gaggcgcgct cctacgaata cggcaaggag atggcctggc ccgcgatcgg gcggcgcgtc
     2281 ctcgcgctca tgcgcgacgt cgtggacgcc cgcgcggatg cggcccacga gcccgtcgcg
     2341 gccggtcaca ctcgcatcgc atgaacctcg gacgacgtct caccgagaac ccgatcctca
     2401 cggcggcaga cgtgccgccg tccctgccga cgctcgaggt cgtctcggtg ttcaacgccg
     2461 cggcggcccg ggtgggggac gagatcatcc tgctcctgcg cgtcgccgag cggccgcgaa
     2521 cggacgtggc gcccggcccc gacgcgctca cgctcgactt cgaccagccg catccggtgc
     2581 tccacccgct gcccgcgcgc taccgggatc aggagctcgt gggcatcgcc ttcctcgacg
     2641 cgcgtcaatc gcccgcgaag gtcgtggtcg cgtacatccc gacggatctg ccgggcctcg
     2701 atctgcgcga tccgcggtcg atccgctatc gcaacaacac cggctacctg cgcatgacga
     2761 acgaggggta catcgacttc ctggcgcaga tcagccatct tcgcgtcgca cgcagccgcg
     2821 acggggtgca cttcaccgtc gacgcggagc cggccatcgc gccgcacacg gagatggagg
     2881 agtacggcgt cgaggacccg cgcgcgacgc tcatcgacgg cgtctggcac atcacctacg
     2941 tgtcggtgtc gcgctggggc atcacgacga gcctcgcgac gacgctcgac ttcaggacgt
     3001 tcgaccgtcg aggcgtcatc ttcctgccgg atcacaagga cgtcgtgatc ttccccgagc
     3061 gtgtgggcgg gaagtacgtg gcgctcacgc ggccgatgcc gcagtcgttc agccggatct
     3121 tcgggatctg gatggcattc agcgacgacc tcgagcactg gggcgagcac gagacgctcg
     3181 cgctgccgcg ctggggctac tgggacgagc tgcgcacggg cgcctccgcc gtcccgttca
     3241 agaccgacca cggctggctc gagctctatc acggcgtgga tcgcaacacc acgtacgcga
     3301 tgggcgcgct gctgctcgac ccgaacgatc cgcggaggat catcgcgcgg tcgcccacgc
     3361 cgatcctcga gccgaccgag gcgttcgagc gtatgggcct gttcaacgac accgtgttct
     3421 cgtgcggcca cgtcgacatg ggcgacggcc ggatccgcct ttactacggg gccgcggact
     3481 cggtgatggc cgccgcggac ttcgagatcg acgagatcgt gagaagcctc gaaccctggc
     3541 acggcgccgc cgagcgcttc gcgctggtct gatgctgcgt cagctcgcgc gcctctccgt
     3601 ggcggtgccg gcggcaggcg accgcgcgac ggcgatcgcg gtgtacgggg attcgacgga
     3661 ccgcgcgctc gtcgcggccg acctgggcca cgagggcgtc gcctgcgtcg atgacgtcgc
     3721 gcgcgcggcc gtgctcctct gcgacctctg ggagtccacg cggctgccgc gcgcgcgcgc
     3781 gtgggcgctg tcgctcgtgg acttcctcct ctatatgcag ctgggcgacg ggcgcttcgt
     3841 gaacttcatc gtcgactggg acggacggca caacgaccgc ggtcccacgt cgttcgcggg
     3901 cggcggcttc tggcacgctc ggggcgtgcg cgcgctcgcc aaggcgtggc tcgcgctggg
     3961 cgacgagcgc gcacggcagg ggctcgcgct cggcctgcct ctcatccgcg agggccgcga
     4021 cgtgccggcg gacgtgcgct cgatccacgc gctcatggcc gtcgagctcg tgcgatcggg
     4081 tcagctcgtc gagctcgcca ccgacctcga gcgctggtgc gcggagatcg tcgcgctccg
     4141 cggcggcgac atcctgctgg acaatcccga cgaggccgag ccgcatctct ggggtcacct
     4201 gcaggaggga gtgctcgccg aggcgggccg cctgctcgac cgtccggacc tgatcgagat
     4261 cgcgcgccgc agtgccgtcg cctacctcgc accgctcatc gagagcggct tcgacctccc
     4321 gatggtgcag ccatatggcg tggcgtcggc cgcgtatggc gtcgagcgtc tcgccgccgc
     4381 gacgcacgat ccggcgttcc gcgggctcgc cgcgaaagcg cgcgcctggt tcggtggccg
     4441 caacagcgca cacctgcccg tgtacgactc gggctccggc cgcgtgcacg acggcatcga
     4501 cgatggggtc ctgaatccgc actccggtgc ggagtcgaac atcgtcgccg cccaggcgct
     4561 catcgccgat cttgtgcgca tcgcaccggc gctcttcccc gtcgtggacg cgtgcttccc
     4621 gcggaccgtg cgcgaacgga tcgccggcgg cgcgctgcag cgcactgcct agcacggtcg
     4681 gcgagcgagc ctcctctagc atttccacga tgcgccggct gctcgcgatc atcgtcgccg
     4741 cgctggcgct cgcggccgcg gtcgcgatgt cctgcggcct gttgccgtcc gatccacggg
     4801 cggcggggac gaaggtgcca cagggggcgc cgctgccgct cgcgatcgag gaggatcccg
     4861 gcgatcccga gccggatctg aacgcgccgc ccgtcgcgct cgcgcctccc ggcggcgcca
     4921 tcatccagca gggcgtcgtg cggatcccga agccgaagat cgacgtgaac gggaagcgcc
     4981 gcgtcgggat ccagatcggc cactggcaga ccgacacagt cccgaaggag tacggcacgc
     5041 gcatccagca gcagaccggc acgagctggg agggcatcac cgaggtcgag gtcacgaccg
     5101 agatcgcgaa ccggctggcc gcgctgctcg aggcgcaggg catcgcggtc gacatcctgc
     5161 ccacgaccgt gccggagggg tatctcgcgg acgtgttcat cgcgcttcat gtcgatggcg
     5221 atggcgtcgg agagctctcc ggattcaaga tggcccacgg cacacgccgc gggcccttcg
     5281 aggaccagct gatgcggacc atcaaggacg tgtacgcgaa ggcgacgggg cttcgctacg
     5341 acacggagca cgtcacccgc gcgatgaccg gctactacgc cttcaactgg ggccgctaca
     5401 tgcacgcgac gtcgccgttc acgccatcga ccatcctgga gctcgggttc ctctcgaacg
     5461 acgacgatcg cgcgctgctc ctcgatcaga ccgacaccat cgcggccgcg ctcagcacgg
     5521 gcatcctcac gttcctcgat gagaccccac ggtcgaagct gttcgggagc gatctgctcg
     5581 taccgcaggc gccgatccgc cagggtccgc tcccgacgcc gacgacgccg ccctgatcag
     5641 ccgcgacgct tcgtcggcct cgtccgtctg cgccgcagat acatccagcg cttcgcgacg
     5701 agaccgtaga ggatcacgcc cgcaagaccg tacgcgaggt cgttgatgct gtcggcgatg
     5761 cccttcacgt tgtgcgtgcc gaggagcacg tcgccgtagt actcctgcac ctcgagcagc
     5821 acgtgcagca tcgtcgagaa gccgacggca gcgagcgcct gcagcccgaa cgcgccgacg
     5881 agcaccatcg cgagggcgcc gggaccgtgg aagtgcggca cgtggtccca ccactcgacg
     5941 gtgttgtaga ggtcgagcga gttacccgac atgtcgagcc agaagatcga gaggtacagg
     6001 tcgtcgtacc agagcggata gcgctcccag cggtggtaga tccagtgcag gatcgggaac
     6061 agcatcgaca gacccaggag gatgccgacg ttgcgcggtc cgagcgcctt gcccgcgaag
     6121 cgctcgtccc cggcgttcac cagggcgtcg atcgtgaacg cgaagatcgc cgtccgcgac
     6181 gcgatgttca cgagcccggc cggctggcgc gcgagcgtgc ccgcacggcc gagcagctcc
     6241 gggatccggc ccatggatcg gcggaccgat cggcgtgcga cgctgcccac gacggccaga
     6301 cgaggcacgg gcggtgccaa tcgccggcag ggcgcgctag gatggcggag caccgcgagg
     6361 tgacgatgag cagggaggcc ggatatgagc gagcagcacg cacgcggcgc cggcctccgc
     6421 taagggcctc ctcccacacc cgacgcgaac cgttcgccgc gtacgcgcat gcccgctcgc
     6481 ttctgccctc cacggaggga ccatgccgcg cgaggcgctg ttcgcgaccg actggctcgt
     6541 acccgccgag cgggacgagc accttggcat cctgaagacc ggcaaggaga gcgaggtcca
     6601 tctcgtcgcg cgacatggcg gcgggcgcac cgtgctgctc gccgagaag
//
LOCUS       DASTZR010000354         2386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_307026, whole genome shotgun sequence.
ACCESSION   DASTZR010000354 DASTZR010000000
VERSION     DASTZR010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307026"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1540
                     /locus_tag="VFV20_07910"
     CDS             <1..1540
                     /locus_tag="VFV20_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288312.1"
                     /translation="RELVAWLAQQPARTRNVVTLDPGDADLVLCQAPVEDTLGGWSTV
                     ASALLLGAKTLRPIETGEGRLASRERLLRQMRAALIEGHRASKAGAAAVVYVPHADAT
                     TLAAVVLAGAGAGYRLRTILYQRDALPGAYGTGAAAATCDFDRDLPLLRDQAAADGGL
                     IEDAMRKGVRDAYLARGEPIREDLAAAAALQSLSAEALLVPLALARAGGVSELELFLD
                     HLRSALADARRGGLARVTLAADPEESGYVVADAHEVTPLDDRVEWAVWGVLSAAREVD
                     TRSLLRRTYALFRGVETPDRELIERVVASYASQSEEGKWRLADVDGLVARQASQAQVL
                     ADLIDAGHRLGFKVHIGRDLQRRALPPSHADRGRTVVDLLADTERLSSPARSLRGPAD
                     ALESVDCVWYERGRMAFLWQVEWTARLHRSIVSLGEAIPDDDRVFRFLAVADERTTLV
                     AEKLERSPGLAELVRRRGWRFVKWKPLRGFLADGEAGLDGLESVLGLEPAVEQTGQQL
                     AFRW"
     gene            1512..1898
                     /gene="trxA"
                     /locus_tag="VFV20_07915"
     CDS             1512..1898
                     /gene="trxA"
                     /locus_tag="VFV20_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU5288313.1"
                     /translation="MASNSRSDGESPRRTRNVTKPTVVSDQTFETEVLKSDTPVLVDF
                     WATWCGPCRMVAPILEEIASEQSAKVRIAKLDVDANPITAGRFGVRSIPTMILFKNGR
                     ETQRVVGYMPKERLLQLLNPHIATAA"
     gene            1895..2119
                     /locus_tag="VFV20_07920"
     CDS             1895..2119
                     /locus_tag="VFV20_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288314.1"
                     /translation="MRDSSGAAGPKILDPVCDMIVAVNDARENGLTLEMDDREYAFCS
                     PGCLKTFAKSPTRFRAKVDAWVAANPSLTA"
     gene            complement(2116..2280)
                     /locus_tag="VFV20_07925"
     CDS             complement(2116..2280)
                     /locus_tag="VFV20_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288315.1"
                     /translation="MDGAGIFVVLAICLVTAAGLGLATATLFARATDAHAAEMTLDQD
                     AQAEPEALEG"
BASE COUNT          354 a          834 c          848 g          350 t
ORIGIN      
        1 ccgcgagctc gtcgcatggc tcgcgcagca gcccgcgcgc acgcgcaacg tcgtcacact
       61 cgatccgggt gacgccgacc tcgtcctctg ccaggcaccg gtcgaggaca cgctgggcgg
      121 ctggtcgacc gtcgcgagcg cgctcctgct cggcgcgaag acgctgcgcc cgatcgagac
      181 cggtgaggga cgcctcgcgt cgcgcgaacg cctgctgcga cagatgcggg cggcgctcat
      241 cgaaggccat cgcgcgtcga aggccggggc ggcggcggtc gtgtacgtcc cgcacgccga
      301 cgcgaccacg ctcgccgcgg tcgtcctcgc gggtgcgggc gccggctacc ggctgcgcac
      361 catcctctac cagcgcgacg cgctgccggg tgcgtacggc accggcgcgg cggcggcgac
      421 gtgcgacttc gaccgcgacc tcccgctgct gcgcgaccag gcggcggcgg acggcggtct
      481 catcgaggac gcgatgcgga agggtgtgcg cgacgcgtac ctcgcgcgcg gcgagccgat
      541 ccgcgaggat ctcgccgccg cggccgcgct gcagtcgctc tcggcggagg cgctgctcgt
      601 cccgctcgcg ctcgcgcgcg ccggcggagt gtccgagctc gagctcttcc tcgaccatct
      661 gcggtcggcg ctcgccgacg cgcgccgcgg cggactcgct cgggtgacgc tcgcggcgga
      721 cccggaggag agcggctacg tggtcgcgga cgcgcacgag gtcacgccgc tggacgaccg
      781 cgtggagtgg gcggtgtggg gcgtgctgtc cgccgcgcgc gaggtcgaca ccaggtcgct
      841 gctgcgccgg acgtacgcgc tcttccgcgg agttgagacg cccgaccgtg agctcatcga
      901 gcgcgtcgtc gcgtcctacg cgtcgcaatc cgaggagggg aagtggcggc tcgcggacgt
      961 cgacggcctc gtcgcgcgcc aggcgtcgca ggcgcaggtc ctcgccgatc tcatagacgc
     1021 tggacatcgc ctcgggttca aggtgcacat cggtcgcgac ctgcagcgac gcgccctgcc
     1081 gccgtcgcat gccgatcgcg gccgcaccgt cgtcgatctg ctcgccgata ccgagcgcct
     1141 ttcgagtccc gcgcggtcgc tgcgcggccc cgcggacgcg ctggaatcgg tggattgtgt
     1201 ctggtacgag cgcggccgga tggcctttct gtggcaggtg gagtggaccg cccgcctgca
     1261 ccgcagcatc gtgtcactcg gtgaggccat acccgacgac gatcgggtct ttcgcttctt
     1321 ggccgtggcc gacgagcgca cgacgctggt ggcggagaag ctggaacgat ccccgggcct
     1381 cgctgagctc gttcgacgcc ggggctggcg gttcgtaaag tggaagccgc tgcgaggctt
     1441 cctggcggac ggcgaagcgg gcttggacgg gctcgaaagc gtgcttggcc tcgaacctgc
     1501 ggtggaacaa actggccagc aactcgcgtt cagatggtga aagcccgagg aggacgagaa
     1561 acgtgacgaa gccaacagtc gtgagcgatc agaccttcga gaccgaggtc ctgaagtccg
     1621 acacacccgt tctcgtcgac ttctgggcga catggtgcgg accctgccgc atggtcgccc
     1681 cgatcctcga ggagatcgcg agcgagcaga gcgccaaggt gcgcatcgcg aagctcgacg
     1741 tggacgcgaa tccgatcacg gcggggcgct tcggtgtccg ctcgatcccg acgatgatcc
     1801 tcttcaagaa cggccgcgag acgcagcgtg tcgtcggcta catgcccaag gagcgtctgc
     1861 tccagctgct caacccgcac atcgcgaccg cggcgtgaga gacagcagcg gcgccgctgg
     1921 accgaagatc ctcgatccgg tctgcgacat gatcgtcgcg gtgaacgacg cgcgcgagaa
     1981 cggcctgacc ctcgagatgg acgaccgcga gtacgcgttc tgctcaccgg gctgcctcaa
     2041 gaccttcgcc aagtcgccga cgcgctttcg cgcgaaggtc gacgcctggg tcgcagcgaa
     2101 cccctctctg accgcctaac cctccagggc ttcgggctcg gcctgcgcat cctggtccaa
     2161 cgtcatctcc gcggcatgcg cgtcggtggc acgcgcgaac agcgttgcgg tggccagtcc
     2221 cagccccgcc gcggtgacga ggcagatcgc caggactacg aagatccccg cgccgtccat
     2281 gcgatctcca ttggccccac ggtcagctcg cgatatcgag cgcgaaacgg agggatccga
     2341 cgccgctacc gcaggcgacg aggagctgca cgtcgtacgt gccggt
//
LOCUS       DASTZR010000355         6238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1217049, whole genome shotgun sequence.
ACCESSION   DASTZR010000355 DASTZR010000000
VERSION     DASTZR010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6238
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217049"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VFV20_07930"
     CDS             complement(<1..630)
                     /locus_tag="VFV20_07930"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5288316.1"
                     /translation="MTGHGKGERRANDRRGFHDRRKHKLPPPGGIERRANQDRRAVER
                     RTVGQRLDVILDVTRRLMAVTDLDALLHTMAEATADLLAADRATIYVVDRERNELWSR
                     VALGAGEIRFPIGVGIAGSVARTGETINIADAYKDARFNPEPDRLSGYHTKSLLTFPM
                     KGNEGRVIGVFQVVNKRGGRPFTDTDEETLTALGASAAVAVENAQLISEQ"
     gene            complement(659..1147)
                     /locus_tag="VFV20_07935"
     CDS             complement(659..1147)
                     /locus_tag="VFV20_07935"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5288317.1"
                     /translation="MLDDKIALIQVKLGHLSVQHDREAYLRGLVDLCSQLVESERCTV
                     YVVDEQKKELWARVAQRTATEIRMPLGQGLAGQAALSGETINVPDAYADPRFDRNIDL
                     RTGFRTLNMLVVPVWGSAGKPVGVIQALNKRSGTFERRDQMLLEQVAETVAPVLEKLR
                     SA"
     gene            complement(1152..3149)
                     /locus_tag="VFV20_07940"
     CDS             complement(1152..3149)
                     /locus_tag="VFV20_07940"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU5288318.1"
                     /translation="MLSRLLAKRQTRQRAVIAGLAVIAVLIGALAQLTEALDTTLQAA
                     VYDRAIALSPATVRNQITIIALDDPTLRQYGVYPLPRRAYADMLRALRPLNPTVIAFD
                     VAFYDRSTSPADDAALAAAIKDAGNVILAMQGVQEIAVGDHRTKYASLQMPLPELREG
                     AIGLGAVDINPDPDHIVRDSQIVIEGPDGTRYYGLPLVAAAKHLRADLGAATIEGDRL
                     TMPAPLAPRVMPLNRGGGMAIYFGAPPATPTYALKEAGKAPCSIAGEFCVVSMADVIA
                     GSVPKNLIQGRTVFIGAHSISAVPDDYPVPHSPGQKMFGVEVWANAAQSIFTNRYPVL
                     KQGFVTTLVELLVVTLLGMLFIVRWHLRGFLYAVGLAIVFTGGAYVLFALQTTSDVGS
                     GPVEVPSIGYVIPSAFWWVVALGYLVVEERRAVNRTQSTFGRFVTPAVARTILAMEEE
                     GHLGLGGEDRTVTVLFGDIRGFTTISEGMTPATLLGHLNRYFDGMVEVVNRFDGTVNK
                     YNGDNIMVIWNAPLEVPDHARKAVECALEMQKWIQSERTKGGPDVSFGFGINTGRVVA
                     GFLGAKGRMEYTVIGDTANVASRLTSSDIARRDQVACSAETLAALGTDVASVDLGAIA
                     VKGRAEPVRCFQIDRIGTVANPNPAPPPDMPIAKAAVAGYH"
     gene            complement(3187..4266)
                     /locus_tag="VFV20_07945"
     CDS             complement(3187..4266)
                     /locus_tag="VFV20_07945"
                     /inference="COORDINATES: protein motif:HMM:NF003367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[LysW]-lysine hydrolase"
                     /protein_id="HEU5288319.1"
                     /translation="MATALDERDLALVRGLVEIPSVSREEGAAVEWLVARMRERGFRA
                     SVDEAGNAVGEIGDGPRHVVLLGHIDTVPGIFAVRVEGGELVGRGAVDAKGPLAAFVA
                     ASTHVVPGVRVTVVGAVEEESPTSKGARHRATLPAPDWCVIGEPSGWDAVTIAYKGRL
                     GLRISLTRDARHGAAPGPTCTEEALSLWSRILAASDERTKGETGFAHLDCRLEGIQGG
                     MTDGLTERAELRVGFRVPPGIATADLAAEVTRVAAAHDGDARPTVEQISSEEPARTSR
                     TSALARAFVGAIAAAGGSVTFKVKSGTSDMNILAPAWGCPMVAYGPGDSRYDHTPVER
                     LSLEDYARGIATLRGVLERAANRSI"
     gene            complement(4263..5447)
                     /locus_tag="VFV20_07950"
     CDS             complement(4263..5447)
                     /locus_tag="VFV20_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5288320.1"
                     /translation="MPLSEVVPSRAVAREDAHTSGLYQKRPVVIVRGDGATLYDEDGR
                     AYIDCVGGQGMASLGHGNRVVADAIAAQARTLASCTELFYNDRRAELYDALSQILPRE
                     LDRFFLCNSGAEAVEGALKFARMATKRPRVVAAMRGFHGKTMGALAATWGPEYREALG
                     GEGLFPGSAHVPFNKPEALDAAITADTAAFIVELVQGEGGVRPATTEFVAEAARLCRE
                     RGALLVIDEVQTGFCRTGTMFALEQYGVVPDLLCMAKAIAGGVPMGAIAFSRAVGDLP
                     KRSHSTTFGGNPLACAASIAAIGEMRRLDLARAARERGAQLMSGLSAIKSEKIREIRG
                     LGLIVGIDLKENSGPSIKALQDEGVLTLGAGPTVVRYLPPLVITAEQIDRVIAATAKV
                     LA"
     gene            complement(5468..>6238)
                     /locus_tag="VFV20_07955"
     CDS             complement(5468..>6238)
                     /locus_tag="VFV20_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[LysW]-aminoadipate kinase"
                     /protein_id="HEU5288321.1"
                     /translation="RAVVKAGGSKGVSRDAVADLVAQVARQDEIVLVHGASAETDRIA
                     AALGVPQQQITSPSGHVSRRTDRRTLEVFAMAALGVENFEYVEKLQQRGIDAIGLSGL
                     SGRLLVAKKKDVRAVLDGKTIILRDDYTGTVEAVNLPLLAQFIAPGRVPVVAPLALST
                     ENEALNVDGDRVAARIAVAIDADALLILTNVPGLLRDVNDPASLVPEATLEEAERLAE
                     GRMKKKILAAREALEGGVDEVVIRSAVGDPSVRTVIHG"
BASE COUNT          889 a         2207 c         2198 g          944 t
ORIGIN      
        1 ctgctcggag atgagctgcg cgttctcgac cgcgaccgcc gccgacgctc cgagggcggt
       61 gagggtctcc tcgtcggtgt cggtgaacgg gcggccgcca cgcttgttca ccacctggaa
      121 cacgccgatc acacggccct cgttgccttt catcgggaag gtcaggaggc tcttggtgtg
      181 atatcccgac aggcggtcgg gctcggggtt gaagcgcgcg tccttgtagg cgtcggcgat
      241 gttgatcgtc tcgccggtgc gcgcgacgct ccccgcgatg ccgacgccga tcgggaagcg
      301 gatctcgccc gcgcccagcg cgacgcggga ccacagctcg ttgcgctcgc gatcgacgac
      361 gtagatggtg gcgcgatcgg ccgccagcag gtccgcggtg gcctcggcca tggtgtgcag
      421 cagcgcgtcg aggtccgtga ccgccatgag ccggcgcgtc acgtcgagga tgacgtcgag
      481 acgctgcccc accgtgcgcc gctcgacggc gcgccggtcc tggttcgcgc gtcgctcgat
      541 gccgcccggc ggcggcagct tgtgcttgcg ccggtcgtgg aagccacgcc gatcgttcgc
      601 ccggcgctcg cccttcccgt gaccggtcac gggtggaccg ctcctcggtc gttcacattc
      661 atgccgagcg cagcttctcg aggacgggcg cgacggtctc cgcgacctgc tcgaggagca
      721 tctggtcgcg ccgctcgaac gtgccgctcc gcttgttgag cgcctggatg acgccgacgg
      781 gcttgccggc cgaaccccac acgggcacga cgagcatgtt cagcgtacgg aagcccgtgc
      841 gcaggtcgat gttccggtcg aagcgcggat cggcataggc gtcgggcacg ttgatcgtct
      901 cgccgctcag cgcggcctgg ccggcgagac cctgacccag cggcatgcgg atctccgtcg
      961 ccgtccgctg ggccacacgc gcccagagct ccttcttctg ctcatcgacg acgtacaccg
     1021 tgcagcgctc cgactcgacg agctgcgagc agagatcgac cagtccgcgg aggtaggcct
     1081 cgcggtcgtg ctgcaccgag aggtgcccga gcttcacctg gatgagggcg atcttgtcgt
     1141 cgagcatctt cctagtgata ccccgccact gccgccttgg cgatgggcat gtcgggcggg
     1201 ggcgcgggat tcgggttcgc gaccgtgccg atgcggtcga tctggaaaca gcgcaccggc
     1261 tcggcacggc ctttcaccgc gatcgccccg aggtccaccg acgcgacgtc ggtcccgagg
     1321 gctgcgagcg tctcggcgct gcaggccacc tggtcgcggc gcgcgatgtc cgacgacgtg
     1381 agccgcgacg ccacgttcgc cgtgtcgccg atgaccgtgt actccatgcg gcccttggcg
     1441 ccgaggaacc cggccacgac gcgcccggtg ttgatcccga agccgaacga gacgtcgggg
     1501 ccgcccttcg tccgctcgct ctggatccac ttctgcatct cgagcgcgca ctcgaccgcc
     1561 ttgcgggcgt ggtcggggac ctcgagcggc gcgttccaga tgaccatgat gttgtcgccg
     1621 ttgtacttgt tcaccgtgcc gtcgaagcgg ttcaccacct cgaccatgcc gtcgaagtag
     1681 cggttgaggt gaccgagcag cgtcgcgggc gtcatcccct cggagatcgt cgtgaagccg
     1741 cggatgtcgc cgaagaggac ggtgacggtc cggtcctcgc cgccgagacc gaggtggccc
     1801 tcctcctcca tcgcgaggat ggtgcgcgcg acggcgggcg ttacgaaccg tccgaacgtg
     1861 ctctgggtgc ggttcaccgc ccggcgctcc tcgacgacga ggtatccgag cgcgaccacc
     1921 caccagaacg ccgatgggat gacgtacccg atcgatggga cctccaccgg gccgcttccg
     1981 acgtcgctgg tcgtctgcag ggcgaagagc acgtaggcgc cgccggtgaa cacgatcgcg
     2041 aggccgaccg cgtagaggaa gccgcgcagg tgccagcgga cgatgaagag catgccgagg
     2101 agggtgacca cgaggagctc gacgagcgtg gtgacgaagc cctgcttcag gaccggatag
     2161 cggttggtga agatggactg cgccgcgttc gcccacacct cgaccccgaa catcttctgg
     2221 cccggcgagt gaggcaccgg atagtcgtcg gggaccgcgg agatcgagtg cgcgccgatg
     2281 aagaccgttc ggccctggat gaggttcttc ggcacgctgc ccgcgatgac gtcggccatc
     2341 gagacgacgc agaactcgcc ggcgatcgag cacggcgcct ttcccgcttc cttcagcgcg
     2401 taggtgggcg tcgccggcgg cgcgccgaag tagatcgcca tgccgccgcc tcgattgagc
     2461 ggcatgacgc gtggcgcgag cggtgcgggc atcgtcagcc ggtcgccctc gatcgtcgcg
     2521 gcacccagat cggcacggag atgcttcgct gcggcgacga gcgggagccc gtagtagcgc
     2581 gtgccgtccg gcccttcgat gacgatctgg ctgtcacgga cgatgtgatc cggatcgggg
     2641 ttgatgtcca cggcaccgag tccgatggcg ccctcgcgca gctcggggag gggcatctgc
     2701 agcgacgcgt acttcgtgcg gtggtcgccg acggcgatct cctgcacgcc ctgcatcgcg
     2761 aggatgacgt tgccggcatc cttgatcgcc gcggccagcg cggcgtcgtc cgcggggctg
     2821 gtcgagcggt cgtagaacgc cacgtcgaac gcgatgaccg tcggattcag cggccggagg
     2881 gcgcgcagca tgtccgcgta cgcgcgccgc ggcaacgggt acacgccgta ctgccgcagc
     2941 gtcggatcgt cgagggcgat gatcgtgatc tggttgcgca cggtcgccgg agagagcgcg
     3001 atggcgcggt catagacggc ggcctggagc gtcgtgtcga gcgcctccgt gagctgcgcg
     3061 agggcgccga tgagcacggc gatcacggcg aggcccgcga tgaccgcgcg ttggcgcgtc
     3121 tggcgcttcg cgagcagccg gctcagcaca accccgccct cctgcccgcc ccgcgcgggt
     3181 ccgggctcag atactacggt tggctgcccg ctccagcact cccctgagcg tggcgatacc
     3241 acgcgcgtag tcctcgagcg acagccgttc gacgggcgtg tggtcgtagc gcgagtcacc
     3301 cgggccgtac gcgaccatcg ggcagcccca cgcgggagcg aggatgttca tgtcggatgt
     3361 tccgctcttt accttgaacg tcacactgcc accagccgcc gcgatcgcgc ccacgaacgc
     3421 acgggcgagc gcgctcgtcc gcgacgtccg tgccggctcc tcggagctga tctgctcgac
     3481 cgtcggcctc gcgtcgccgt catgagcggc tgccacgcgc gtcacctcgg ccgcgaggtc
     3541 cgcggtcgcg atccccggcg gcacgcggaa ccccacacgc agctcggcgc gctcggtgag
     3601 gccgtcggtc atgccgccct ggatgccctc gaggcggcaa tcgagatggg cgaaccccgt
     3661 ctcgcccttc gtccgctcgt cgctcgccgc gaggatgcgg gaccagaggg acagggcttc
     3721 ctcggtgcac gtcggacccg gcgccgcacc gtggcgcgcg tcgcgcgtca gcgagatccg
     3781 caggccgagg cgtcccttgt aggcgatggt gacggcgtcc cagccggagg gctcgccgat
     3841 gacgcaccaa tcgggcgcgg ggagagtggc gcgatggcgc gcgcccttgc tggtcggcga
     3901 ctcctcctcg acggccccga cgaccgtcac gcgcacgccg ggcacgacgt gcgtggacgc
     3961 cgccacaaag gccgcgagcg gtcccttcgc gtccaccgcg ccgcggccga cgagctcgcc
     4021 gccctcgacg cgcaccgcga agatgccggg caccgtgtcg atgtggccga gcagcaccac
     4081 gtgacgcggc ccatcgccga tctcgccgac ggcgttccca gcctcgtcga ccgacgcacg
     4141 gaagccgcgc tcccgcatgc gcgcgacgag ccattcgacg gcggcgccct cctcgcgcga
     4201 gacgctcggg atctcgacga ggccgcggac cagcgcgagg tcgcgctcgt cgagcgcggt
     4261 cgctatgcga gcaccttcgc ggtcgccgcg atcacgcggt cgatctgctc cgccgtgatg
     4321 acgagcggcg gcaggtagcg cacgacggtc gggccggcac cgagcgtgag cacgccctcg
     4381 tcctgcagcg ccttgatcga ggggccggaa ttctccttga ggtcgatgcc cacgatgagc
     4441 ccgaggccgc ggatctcgcg gatcttttcg ctcttgatcg cggagagccc ggacatgagc
     4501 tgcgcgccgc gctcgcgcgc ggcgcgcgcg aggtcgagcc ggcgcatctc gccgatggcg
     4561 gcgatcgagg ccgcgcacgc gagcgggttg ccgccgaacg tcgtcgaatg cgagcgcttg
     4621 ggtaggtcgc cgaccgcccg cgagaacgcg atcgcgccca tcggaacgcc gccggcgatc
     4681 gccttcgcca tgcacagcag gtcgggcacg acgccgtact gttcgagcgc gaacatcgtg
     4741 ccggtccggc agaatcccgt ctgcacctcg tcgatcacca gcagggcgcc gcgctcgcgg
     4801 cacagccgcg ccgcttcggc gacgaactcc gtcgtggcgg gacgcacacc gccctctccc
     4861 tgcacgagct cgacgatgaa cgccgcggtg tccgccgtga tcgccgcgtc gagcgcctcg
     4921 ggcttgttga acgggacgtg cgccgagccc gggaagaggc cctcgccgcc gagcgcctcg
     4981 cggtactcgg gaccccacgt cgcggcgagc gcgcccatcg tctttccgtg gaagccacgc
     5041 atcgcggcga cgacgcgcgg gcgtttcgtc gccatgcgcg cgaacttcag cgcgccctcc
     5101 accgcctcag cgccgctgtt gcagaggaag aagcggtcca gctcacgcgg caggatctgc
     5161 gagagcgcgt cgtacagctc cgcccggcgg tcgttgtaga agagctcggt gcacgacgcg
     5221 agcgtgcggg cctgcgccgc gatggcgtcg gcgacgacgc ggttgccgtg cccgaggctg
     5281 gccatgccct gaccgccgac gcagtcgatg taggcccggc cgtcctcgtc gtacagcgtc
     5341 gcgccgtcgc cgcggacgat caccaccggg cgcttctggt acaggcctga ggtgtgcgca
     5401 tcctcgcgcg ccacggcacg gctcggcacg acctccgaca gaggcatcga gctacgcgtg
     5461 cgctcgctca accgtggatc accgtgcgga cgctcgggtc gccgacggcg gagcggatga
     5521 cgacctcgtc cacaccgcct tcgagcgcct cgcgtgcggc gaggatcttc ttcttcatgc
     5581 gtccctccgc gagccgctcg gcctcctcga gtgtcgcctc ggggacgagc gatgccgggt
     5641 cgttgacgtc gcgcagcagg ccgggcacgt tggtgaggat gagcagcgcg tcggcgtcga
     5701 tcgccaccgc gatgcgcgcg gccacccgat cgccatcgac gttgagcgcc tcgttctcgg
     5761 tcgagagcgc cagcggcgcg accacgggca cgcggcccgg cgcgatgaac tgcgcgagca
     5821 gcggcaggtt gaccgcttcg acggtcccgg tgtagtcgtc gcgcaggatg atggtctttc
     5881 cgtcgaggac cgcacgcacg tccttcttct tggcgacaag gagccgtccg gagagacccg
     5941 acaggccgat ggcgtcgatg ccccgctgct ggagcttctc gacgtactcg aagttctcga
     6001 caccgagggc cgccatcgcg aagacctcga gggtgcggcg atccgtacga cggctcacgt
     6061 gaccggacgg cgacgtgatc tgctgctgcg gcacaccgag cgcggcggcg atgcgatcgg
     6121 tctcggcgga cgcgccgtgg accaggacga tctcgtcctg acgcgcgacc tgcgcgacga
     6181 ggtcggcgac cgcatcgcgc gacacaccct tcgacccacc ggccttgacg accgctct
//
LOCUS       DASTZR010000356         4400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1294216, whole genome shotgun sequence.
ACCESSION   DASTZR010000356 DASTZR010000000
VERSION     DASTZR010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294216"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VFV20_07960"
     CDS             <1..384
                     /locus_tag="VFV20_07960"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU5288322.1"
                     /translation="RCIGCRMCMAACPYGARYFNYVEPPKYDNPFSKPTPEYPVPQQK
                     GTVGKCMFCVHKTDEGQLPACVEACGMKAIYIGDLVEDVATNGDQTVRLSTFLKENDA
                     FRYKEELGTGPRVWYIAGHGQLLDY"
     gene            384..1715
                     /gene="nrfD"
                     /locus_tag="VFV20_07965"
     CDS             384..1715
                     /gene="nrfD"
                     /locus_tag="VFV20_07965"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEU5288323.1"
                     /translation="MERKQHDRYVERAMWPIGRGDRKFWIAVVVLGAMVAAGIYAWIY
                     QIQRGLWVTGLNQKVSWGFYIINCVFFIGMSYGGAMTSAILRLTNAKWRAPLTRLAEA
                     TAVAALLVGAVYPIIDMGRPERFLNILLFAQVGSPVVWDVVAIGTYMIASLVFLYLPL
                     IPDLATTRDSLRMSRLQRIYRILALGWTGTEKQERSLARGIGVMAVLIIPLAVSVHSV
                     LAWLFAVTARGGWHSTIFAPLFVLAAMLSGVAAVILVIAAFRKVYGLQEFITAKHFRY
                     LSFLLIALGVGYLYFMVSEYLTEGYLLDEATAPLLESIFIGSYAPAFWGFTIIGLILP
                     LALTAFSGRYMIARACTASALVIAGMWLKRFLIVVPGMAEPIMPWDWGRYAPSWVEIT
                     ITVGSAAAIPLILIIFFRFFPVMSVHEMEEVEGAPEPRGVVLMPTFADGGK"
     gene            1712..2401
                     /locus_tag="VFV20_07970"
     CDS             1712..2401
                     /locus_tag="VFV20_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU5288324.1"
                     /translation="MMRRLIPFIGAAIATLAIVGAGAGGLLPGGMRLQLPSGASTSTT
                     TTSASGHDASKATEFRALSLVPPAGASKLAVAVTPAAKPEQGYLVSVQVTSPTGKAVG
                     DTTIKFYDVVDLFGQREALIGSAPTDGQGTAVISYLPATKGTHQIVARFGGQGTLGPS
                     VGVATLDATVAAPTYKVDQPSFANFARYVPFGAGFIVLAVWGLIAFSLFATARGVLAG
                     ADRRIRKGDTA"
     gene            2398..2976
                     /locus_tag="VFV20_07975"
     CDS             2398..2976
                     /locus_tag="VFV20_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288325.1"
                     /translation="MILRRILAITATSVFVVGALASCGGGTGETATSATTAPSVAATT
                     QATAAPQKATFLILQGDTVRSPEGLTDEEKVFLSCVQQSRFPQGSRIVWRYRVNAPLT
                     NKALDDKGVKSFDLILPDGKAVAFKYGGHGGTKPNYAEYFWTAGFSIPADYPTGLFNY
                     QVKAVGAEGAIGTFEQMKVPAAQLTVVKAGTR"
     gene            2981..>4400
                     /locus_tag="VFV20_07980"
     CDS             2981..>4400
                     /locus_tag="VFV20_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288326.1"
                     /translation="MRRVVVAIAAATFLAACSATPTPSARSSEAAATAKPAATATTAV
                     ASAAPSTTPQQQLEVVGKFALAPTHGPWDTKVTATATGMKPNTKYDLVWVTGKVTWKL
                     SEDKSKYLGRDAKQIQSVLGSYTTDATGAFTTTFAVPRGFGYAHDVMVVDEGKVIRNK
                     SLFDVDMITTITPTSGPVGTPITIEVKGIGYQAYQNSFQLDYDNAYTGWVSSVTTDGY
                     AKFVIPATGAVGTHIITLGHSDFGAPYMNPNQQPVVASRPFPHLPFTVTEGPAVMPTP
                     AETQGFAQVAGKPVAQGIWSEPYATTVATPVTLNAKALAANSDIELFWTTQVGNRTVT
                     GFEERTSSLGKTKTDANGAFSWTFNMPDDLGGTHTITAKLADKEVAKTTVYILAQAFP
                     LDVARGPAGTKTTLHIKGAGWTETEQIYHVVVDNAYSGYACAFNSKGDLTIYLTMSGA
                     PGWHYIDMYPGIYQGPNDIKPAD"
BASE COUNT          746 a         1576 c         1390 g          688 t
ORIGIN      
        1 cgctgcatcg gctgccgcat gtgcatggcg gcatgcccgt acggcgcgcg ctacttcaac
       61 tacgtagaac cgccgaagta cgacaacccc tttagcaagc cgacgccgga gtacccggtc
      121 ccgcagcaga agggcaccgt cggcaagtgc atgttctgcg tccacaagac ggatgagggc
      181 cagttgcccg cgtgcgtgga ggcgtgcggg atgaaggcga tctacatcgg cgatctcgtc
      241 gaggacgtcg cgacgaacgg cgaccagacg gtgcgcctct cgaccttcct caaggagaac
      301 gacgcgttcc gctacaagga ggagctcggc accggcccgc gcgtctggta catcgcgggt
      361 cacggccagc tgctggatta ctgatggaaa gaaagcagca cgaccgctac gtcgagcgcg
      421 cgatgtggcc gatcggccgc ggcgaccgca agttctggat cgcggtcgtc gtcctcggcg
      481 ccatggtcgc cgcgggcatc tacgcgtgga tctaccagat ccagcgcggc ctctgggtca
      541 ccggcctgaa ccagaaggtc tcgtggggct tctacatcat caactgcgtc ttcttcatcg
      601 gcatgagcta cggcggcgcg atgacgagcg ccatcctgcg gctcaccaac gccaagtggc
      661 gcgcgccgct cacccgcctc gctgaggcga ccgcggtggc ggccctgctc gtcggcgccg
      721 tctacccgat catcgacatg ggccgtcccg aacggttcct caacatcctg ctcttcgcgc
      781 aggtcggctc gcccgtggtc tgggacgtcg tcgcgatcgg cacgtacatg atcgcgagcc
      841 tcgtcttcct ctacctgccg ctcatccccg atctcgccac gacccgcgac tcgctgcgca
      901 tgtcgcggct ccagcgcatc taccggatcc tcgcgctggg ctggaccggc accgagaagc
      961 aggagcggtc gctggcacgc ggcatcggcg tcatggccgt gctgatcatc ccgctcgccg
     1021 tctccgtcca ctcggtcctg gcgtggctct tcgcggtgac ggcccgcggc gggtggcaca
     1081 gcacgatctt cgctccgctc ttcgtgctgg ccgcgatgct gtccggcgtc gccgcggtga
     1141 tcctcgtgat cgcggccttc cgcaaggtgt acggcctgca ggagttcatc accgccaagc
     1201 acttccggta tctctcgttc ctgctgatcg cgctcggcgt cgggtacctc tacttcatgg
     1261 tcagcgagta cctcacggag ggttacctcc tggacgaggc gacggcgccg ctgctcgagt
     1321 cgatcttcat cggcagctac gcaccggcct tctggggctt cacgatcatc ggcctcatcc
     1381 tcccgctcgc gctcaccgcg ttctccggcc gctacatgat cgcccgggcc tgcacggcct
     1441 cagccctcgt gatcgccggg atgtggctga agcggttcct catcgtcgtc cccggcatgg
     1501 ccgagccgat catgccctgg gactggggcc gctacgcccc gagttgggtc gagatcacga
     1561 tcacggtcgg atccgcggcc gccatcccgc tgatcctgat catcttcttc cgcttcttcc
     1621 cggtgatgtc cgtccacgag atggaagagg tcgaaggcgc gcccgagccg cgtggcgtcg
     1681 tcctgatgcc gaccttcgcg gatgggggta agtgatgcgt aggctcatcc cgttcatcgg
     1741 cgcggccatc gcgaccctgg cgatcgtcgg cgcgggcgcc ggcggcctcc tccccggtgg
     1801 gatgcggttg cagctcccga gcggcgcgag cacctcgacc acgacgacca gcgcctcggg
     1861 acacgacgcc tcgaaggcca ccgaattcag ggccctgtcg ctcgtgccgc ccgccggtgc
     1921 gtcgaagctc gcggtcgcgg tcacgcccgc ggcgaagccc gagcagggct acctcgtgtc
     1981 ggtacaggtc acgagcccga cgggcaaggc ggtgggcgac accacgatca agttctacga
     2041 cgtggtcgac ctcttcggcc agcgtgaggc gctcatcgga agcgcaccca cggacggcca
     2101 gggcaccgcg gtcatcagct acctgcccgc gaccaagggc acgcatcaga tcgtcgcgcg
     2161 gttcggcggc cagggcacgc tcggtccatc cgtcggcgtc gcgacgctcg acgcgaccgt
     2221 cgccgcgccg acgtacaagg tcgaccagcc gagcttcgcc aacttcgcgc gctacgtccc
     2281 cttcggcgcg ggcttcatcg tgctcgcggt atggggactc atcgccttct cactgttcgc
     2341 gaccgcgcgc ggcgtcctcg ccggcgcgga tcggagaatt cgaaaggggg acaccgcatg
     2401 atcctgcgcc gcatcctcgc gatcaccgcg accagcgtct tcgtcgtcgg cgcgctcgcc
     2461 tcgtgtggag gcggcaccgg cgagacggcg acctccgcga ccacggcccc gtccgtcgct
     2521 gcgacaacgc aagcgacggc agcgccgcag aaggccacgt tcctgatcct gcagggcgac
     2581 actgtccgca gccccgaggg cctcacggac gaggagaagg tcttcctctc ctgcgtccag
     2641 cagagccgct tcccgcaggg ctcccgcatc gtgtggcggt accgcgtcaa cgccccgctg
     2701 accaacaagg cactcgatga caagggcgtc aagtcgttcg atctcatcct cccggacggc
     2761 aaggctgtcg cgttcaagta cggcggccac ggcggcacca agccgaacta cgccgagtac
     2821 ttctggaccg ccggcttcag catcccagcg gactacccga cagggctctt caactaccag
     2881 gtcaaggcgg tcggcgctga gggcgccatc ggcaccttcg agcagatgaa ggtcccggcg
     2941 gctcagctca cggtcgtcaa ggccggaacg aggtaacgac atgagacgcg tcgtcgtcgc
     3001 gatcgcagcg gcaacattcc tcgcagcctg cagcgctaca ccaactccgt ccgcgcggag
     3061 ctcggaggcg gctgccacag caaagcccgc cgcgaccgcg acgacggccg tcgcatccgc
     3121 ggctcccagc acgacaccac agcagcaact cgaggtcgtc ggcaagttcg ccctcgcccc
     3181 gacgcacggc ccgtgggaca cgaaggtcac cgccacggcg acgggcatga agccgaacac
     3241 caagtacgac ctcgtgtggg tcaccggcaa ggtgacctgg aagctctccg aggacaagag
     3301 caagtacctc ggccgcgacg cgaagcagat ccagagcgtg ctcggttcgt acacgaccga
     3361 cgcgaccggc gcgttcacca cgacgttcgc cgtgccgcgc ggcttcggtt acgcgcacga
     3421 cgtgatggtc gtcgacgaag ggaaggtcat tcgcaacaag tcactcttcg atgtcgacat
     3481 gatcacgacg atcaccccga cgagcggccc ggtgggcacg cccatcacga tcgaggtgaa
     3541 gggcatcggc taccaggcgt accagaacag cttccagctc gactacgaca acgcgtacac
     3601 cgggtgggtc tcgtcggtca cgaccgacgg ctacgcgaag ttcgtgatcc cggcgaccgg
     3661 cgccgtgggc acgcacatca tcacgctcgg ccactcggac ttcggcgcac cgtacatgaa
     3721 ccccaaccag cagccggtcg tcgcatcgcg tccgttcccg cacctcccgt tcaccgtcac
     3781 cgaagggccc gcggtcatgc cgacgcccgc ggagacgcag ggcttcgcgc aggtcgccgg
     3841 taagccggtg gcgcagggca tctggtcgga gccgtacgcg acgacggtcg cgacgcctgt
     3901 gacgctgaac gcgaaggcgc tggccgcgaa cagcgacatc gagctcttct ggacgacgca
     3961 ggtcggcaac cgcaccgtca ccggattcga ggagcgcacc tcctcgctcg gcaagacgaa
     4021 gaccgacgcg aacggcgcgt tcagctggac gttcaacatg cccgacgatc tcggcggcac
     4081 gcacaccatc accgcgaagc tggctgacaa ggaagtcgcc aagacgacgg tgtacatcct
     4141 ggcccaggcg ttcccgctcg acgtggcgcg cggcccggcc ggtaccaaga cgacgctcca
     4201 catcaagggc gccggctgga ccgagaccga gcagatctac cacgtggtcg tggacaacgc
     4261 gtactccggc tacgcctgcg ccttcaacag caagggcgac ctcacgatct acctcacgat
     4321 gtcgggcgcg ccgggctggc actacatcga catgtacccc ggcatctacc agggtccgaa
     4381 cgacatcaag cccgccgact
//
LOCUS       DASTZR010000357         2683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_563953, whole genome shotgun sequence.
ACCESSION   DASTZR010000357 DASTZR010000000
VERSION     DASTZR010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563953"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VFV20_07985"
     CDS             <1..352
                     /locus_tag="VFV20_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288327.1"
                     /translation="SPGPPQVDVILYTPADGTLDVSDLTTGGKKVGRITVDPNADPAS
                     AVIDLTDIVRRRGLESFGIRLQAHGLDLPELFEEPEDPTHTDSEEGEEHEDDDAPRAP
                     FSASFSFDLVLDNQ"
     gene            complement(477..851)
                     /locus_tag="VFV20_07990"
     CDS             complement(477..851)
                     /locus_tag="VFV20_07990"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEU5288328.1"
                     /translation="MNGIAIRLLLDTDVLVDSLRGARRLAVGDARMAYSVVTLCELFA
                     GSGTDERVVRALLSPFTPIAVDVEIAELAARIRRATGIRTPDALIAGTALLHQLDLVT
                     RNRRDFGRVDGLAVRDPGTLAR"
     gene            1129..>2683
                     /locus_tag="VFV20_07995"
     CDS             1129..>2683
                     /locus_tag="VFV20_07995"
                     /inference="COORDINATES: protein motif:HMM:NF019353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEU5288329.1"
                     /translation="MGRAGLGAYVELHSHSSYSFLDGASSVEDLVTAAAERGMDALAL
                     TDTNGLYGAVRFWNAAREAGIKPIHGVELATLDFGHLVLIAKDEIGWRSLCRTVSAAQ
                     LAGEKRRPRATLALLRENAEGLFALTGCARGAVPRAMRAGDVDAARESLATLAGIFGE
                     RCFVELSDHLGPDDPALCDALAELAREQGLGAVVTNDVHYAWPEGRRLHDVLRCIDLG
                     VTLDEAGDRLEANGEYWLKDEAVLRERLGRHDEAFRNARLIADACTLDLERIGPGGVM
                     AHGGAGQELGERAMAGRERLPGFAAPEGHTAFSFLYALCQDGARAKYGRMTPRVSRQL
                     AHELDVIERCGLAEFFLINWDIVRFCKERQIPAQGRGSAADSIVAYVLDITKVDPIAH
                     DLLFERFLTEDSRTMPDIDLDIATNDREEVIQYVYKKYGERYAAMVCNVVTYRARSAS
                     REVAKALGFRDETIDRMARSIDQYHVDPTGRPSAAMHPAYKHPNREAEHGWEAPRTLP
                     DTLEDLAATM"
BASE COUNT          435 a          948 c          930 g          370 t
ORIGIN      
        1 gtcaccgggg ccgccgcagg tcgacgtcat cctctacacg cccgcggacg gcacgctcga
       61 cgtgagcgac ctcaccacgg gcgggaagaa ggtcggacgc atcacggtcg acccgaacgc
      121 ggacccggcg tccgcggtca tcgacctcac cgacatcgtg cggcgccgcg ggctcgagag
      181 cttcggcatc cgtctccagg cgcacggcct ggacctcccg gagctgttcg aggaacccga
      241 ggatcccacg cacaccgaca gcgaggaggg ggaggagcac gaggacgacg acgcgccgcg
      301 cgcgccgttc tccgcgtcct tctcgttcga tctggtgctc gacaaccagt agcacgcgcg
      361 cggcattgcg accacgaacg ggggcgctgc ggcgcccccg ttccctgtct tcccgccatg
      421 gccgaagagt gatagaacag atgttctacg agaaacggca ggggagcgcc gatgcgtcag
      481 cgcgcgagcg tgcccggatc gcgcacggca agtccgtcga cacgtccgaa atcgcgccgg
      541 tttcgcgtca ccaggtcgag ctggtggagg agcgcggtcc cggcaatgag cgcgtcgggc
      601 gtacgtatgc cggtcgctcg ccggatgcgt gctgcgagct ccgcgatctc gacgtcgacc
      661 gcgatcggag tgaacgggct gagcagcgca cgtacgacgc gctcatcggt gccgctgccg
      721 gcgaagagct cgcagagagt gacgacggag tacgccatcc gcgcgtcgcc aacggcaagg
      781 cgtcgggcgc cgcgaaggct atcgacgagg acgtcggtgt cgagcagaag acgtattgcg
      841 atcccattca tcgcgagagg gcaccttcag gtgcggcagc gccccgaacg tgctgtcgat
      901 gatcgcctgc acctcggcgc gcgagggctt gccgaggtac gcatcgaggg cgtcccgaac
      961 gatctccgcc atcgtcgttc gctcgcgcgt cacgcgctcg tcgatccgtc gacgctgctc
     1021 ggcggtgaga tagatctgtg tacgggtcgc acctttcata tacagcaaat tatacatcac
     1081 aaggcggcgt aaaccatgtc cgcaccagaa cgatcgacag cgacccgcgt gggtcgagcg
     1141 gggctcgggg cctacgtcga gctccactcg cacagcagct acagcttcct cgacggggcg
     1201 tcgtcggtcg aggacctcgt gacggcggcg gccgagcgcg gcatggacgc gctcgcgctc
     1261 accgatacga acggcctcta cggcgcggtg cgcttctgga acgccgcgcg ggaggccggc
     1321 atcaagccga tccacggcgt cgagctcgcc acgctcgact tcggccatct cgtgctcatc
     1381 gcgaaggacg agatcggctg gcgcagcctc tgccgcacgg tgtccgcggc gcagctcgct
     1441 ggcgagaagc gcaggccgcg cgccacgctc gcgctgctgc gcgagaacgc cgaggggctc
     1501 ttcgcgctca ccggctgcgc gcgtggagcg gtgccgcgcg cgatgcgcgc gggcgatgtc
     1561 gacgcggcgc gcgagtcgct cgccaccctc gccggcatct tcggcgagcg ctgcttcgtc
     1621 gagctcagcg atcatctcgg ccccgacgac cccgcgctct gcgacgcgct cgcggagctc
     1681 gcgcgcgagc agggcctcgg cgcggtcgtg acgaacgacg tccactacgc gtggcccgag
     1741 ggccgccgcc tgcacgacgt gctgcgctgc atcgacctcg gtgtgacgct cgacgaggcg
     1801 ggggaccggc tcgaggcgaa cggcgagtac tggctgaagg acgaggccgt gctgcgcgag
     1861 cggctcggcc gtcacgacga ggcgttccgc aacgcgcggc tcatcgccga tgcgtgcacg
     1921 ctcgacctcg aacgcatcgg gccgggcggc gtcatggccc acggcggcgc gggacaagag
     1981 ctcggcgagc gcgccatggc cgggcgcgag cggctgcccg gcttcgccgc gccggagggc
     2041 cacaccgcgt tctccttcct ctacgcgctc tgccaggacg gggcgcgcgc gaagtacggc
     2101 cgcatgacac cgcgcgtgtc gcggcagctc gcgcacgagc tcgacgtcat cgagcgctgc
     2161 gggctcgcgg agttcttcct catcaactgg gacatcgtgc ggttctgcaa ggagcgccag
     2221 atccccgcgc agggccgcgg atccgccgcc gactcgatcg tcgcgtatgt gctcgacatc
     2281 accaaggtcg acccgatcgc gcacgacctc ctcttcgagc gcttcctcac cgaggacagc
     2341 cgcaccatgc ccgacatcga cctcgacatc gcgacgaacg accgcgagga ggtcatccag
     2401 tacgtctaca agaagtacgg cgagcgctac gccgcgatgg tctgcaacgt cgtcacctac
     2461 cgcgcgcgct cggcctcgcg cgaggtggcg aaggcgctcg gcttccgcga cgagacgatc
     2521 gaccgcatgg cccgctcgat cgaccagtac cacgtcgatc cgacgggccg cccgagcgcg
     2581 gcgatgcacc ccgcgtacaa gcatccgaac cgggaggcgg agcacggctg ggaggcaccg
     2641 cgcacgctgc cggacacgct cgaggacctc gcggcgacga tgt
//
LOCUS       DASTZR010000358         4812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_328426, whole genome shotgun sequence.
ACCESSION   DASTZR010000358 DASTZR010000000
VERSION     DASTZR010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328426"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="VFV20_08000"
     CDS             complement(<1..852)
                     /locus_tag="VFV20_08000"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU5288330.1"
                     /translation="MRRAVVAVLAVLAAAALITFPIWTAEWESSRYATTILRDMLVLA
                     IFALSLDLLVGSAGMPSLGHAAFFGAGAYAAAIVAQRTGNDDLALGLGAAIVVSGILA
                     LLIGWFAVRAAGIFFLMLTLAFAQMVFAVAFQAADLTGGSNGFAGVHRPTFLGLDFNA
                     PDALFQLVAGIFVLVLLLLWRVRSSTYGRALVGLRENERRMRALGYDAARLKLSAFVL
                     AGMIAGVGGALSGYALRFVSTNDVSVVRSIQAFVSVLIGGTGTLLGPVVGAAVVLYIE
                     RVLPSSIS"
     gene            complement(849..1721)
                     /locus_tag="VFV20_08005"
     CDS             complement(849..1721)
                     /locus_tag="VFV20_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU5288331.1"
                     /translation="MSPEVAVQQALNGISFGALLFILAAGLSLIFGMMDVVNLAHGAF
                     YMLGAYVTLTLVQRTGQFWPALVIAPLAVGLLGLVIEPLLLRRLRGRHLDQVLLTIGV
                     SLVIADLLGVTFGREIHSIPLPTGLDRSIAIAGGDYPVYRLFVIGFGIAMAAVLALVH
                     RRTQVGALIRAGVDDAQMLDALGVDIDRLFANTFAVGAALAGLAGVIAAPVFGIFPGM
                     DVDALIYSLIVVVVGGLGTLSGALAGSGLVGPADTFGRVLFPQFALALIFAIMAFVLL
                     LRPTGLLGRVRIAR"
     gene            complement(1821..3101)
                     /locus_tag="VFV20_08010"
     CDS             complement(1821..3101)
                     /locus_tag="VFV20_08010"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5288332.1"
                     /translation="MNGSNSSRLTRRKFLVASSAAALAAACGPAATPGASGAPATSAA
                     AATSATVSAKPLKIGQLLPFTKVYAELGNSMKRATDLYLKQKGNKLGGRTVAPPIYED
                     EANDPAVGAQKTQKFIEQDQVDVMMGIVATPIAYAIRNAVDSAKMVFIETNAGGNALT
                     RTTTDCKPACKSKYIFRTSFSSWQISNPIGTYFATKKGVKEAFTFVSDYGFGNESAAD
                     FTNAFQAAGGKVTGTLKTPLPTADFLPVVTQLKNQPTKDIYSFFSGTDAINYIKAWNQ
                     LGMPAAGYRMNGAGFLTEQDVLKEVKELAVGAVTSLFWAVTLDNAENKAFVEAYQKEY
                     NLLPDVFAVQAWDGMQALDLALQKTNGDTSNKDAFIAALEGVKFKSPRGDFSFDKDTH
                     NVIHDIYIREVKVQGGQVVNTITDKIAAVTDPGK"
     gene            complement(3217..4419)
                     /locus_tag="VFV20_08015"
     CDS             complement(3217..4419)
                     /locus_tag="VFV20_08015"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEU5288333.1"
                     /translation="MSQSPRDAVILGYVRTPIGRYGGVLASIRPDDLASHVVRAVLDR
                     TDIGDADVDEVIFGATNQAGEDNRNIGRMAALLAGLPDTVPGVTLNRLCGSGLEAVND
                     AARRIRAGEADIVVAGGVESMTRAPLVMLKPSDAFPRGERTIYDTTIGWRMTNPRMID
                     LGYHPIGLGETAENVCVQEHVSREDQDAYALRSQQRYAKAKEAGFFKGEIQPVHNGKE
                     LVSDDEHPRPDVTLEKLAKLRPAFKKDGTVTAGNSSGINDGAAALIVGSREKAAALGL
                     RPLGRVVASASAGVHPDYMGLGPIPAVRKLVQRTRVAVDECDVIELNEAFAAQVLPCM
                     RQLGLPEDRTNPNGGAIAVGHPIGMSGARLAGTALRELERRAGRYAIATMCIGVGQGL
                     ATLFERES"
     gene            complement(4424..>4812)
                     /locus_tag="VFV20_08020"
     CDS             complement(4424..>4812)
                     /locus_tag="VFV20_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU5288334.1"
                     /translation="AWSRVGLAPDAGAAYFLPRIVGWGRAMDMILTGDAVDAEEALRI
                     GLVTRVWSDTEFAREWKAYAMTIAQGATGAYALSKESLNAAWDRDLRSFLELEVELQD
                     RAGRTRDYAEAIKAFAEKRRASFEGK"
BASE COUNT          774 a         1641 c         1689 g          708 t
ORIGIN      
        1 cgagatcgat gacgggagca cgcgttcgat gtagaggacg acggccgcgc cgaccacggg
       61 accgagcagc gtcccggtgc cgccgatgag caccgagacg aacgcctgga tcgatcgcac
      121 gacgctcacg tcgttggtcg aaacgaagcg cagcgcgtag ccggaaaggg caccgcccac
      181 cccggcgatc atgcccgcga gaacgaaggc cgacagcttg aggcgcgcgg cgtcatatcc
      241 gagcgcgcgc atgcgccgtt cgttctcgcg cagccccacg agcgcgcgcc cgtacgtcga
      301 cgagcgcacg cgccagagca gcaggaggac gagcacgaag atgccggcga ccagctggaa
      361 cagcgcgtcc ggcgcgttga agtcgaggcc gaggaacgtc ggccggtgca cgccggcgaa
      421 tccgttcgac cctccggtca ggtccgcggc ctggaacgcc accgcgaaca ccatctgcgc
      481 gaacgcgagc gtgagcatca ggaagaagat cccggcggct cgcaccgcga accatccgat
      541 gagcagcgcc aggatccccg atacgacgat cgcggcgccg agcccgagcg cgagatcgtc
      601 gttcccggtg cgctgcgcga cgatcgcggc ggcgtaggcc cccgcgccga agaacgcggc
      661 gtgcccgagg gagggcatgc ccgccgatcc cacgagcagg tcgagcgaga gcgcgaagat
      721 cgcgagcacc agcatgtcgc gcaggatcgt ggtcgcgtag cgcgacgact cccactccgc
      781 cgtccagatc gggaacgtga tgagggcggc ggcggcgagc acggcgagga ccgcgacgac
      841 ggcgcgcctc atcgcgcgat gcgcacgcga ccgaggagtc ccgttggccg cagcagcagg
      901 acgaacgcca tgatcgcgaa gatgagcgcc agcgcgaact gcgggaagag cacgcggccg
      961 aaggtgtccg cggggccgac caggccggag ccggcgagcg cgccggacag cgtccccagt
     1021 ccgccgacga ccacgacgat cagcgagtag atgagcgcgt cgacgtccat ccccgggaag
     1081 atgccgaaca ccggcgcggc gatcactccc gcaagtcccg cgagcgcggc gccgaccgcg
     1141 aacgtgttcg cgaagagacg gtcgatgtcg acgccgagcg cgtcgagcat ctgcgcgtcg
     1201 tcgacgcccg cgcggatgag cgcgccgacc tgcgtgcggc ggtgcacgag cgcgagcacg
     1261 gcggccatcg cgatgccgaa gccgatgacg aacagccggt acacggggta atcgccgccc
     1321 gcgatggcga tcgagcggtc gagtccggtc gggagcggga tcgaatggat ctcgcggccg
     1381 aaggtcaccc cgaggaggtc ggcgatgacg agcgacacgc cgatggtgag gagcacctgg
     1441 tcgaggtggc ggccgcgcag gcgccgcagc aggagcggct cgatgacgag acccagcagg
     1501 ccgacggcga gcggcgcgat cacgagcgcc ggccagaact ggccggtccg ctggacgagg
     1561 gtgagggtca cgtatgcgcc gagcatgtag aacgcgccgt gcgcgaggtt gacgacgtcc
     1621 atcatcccga agatcaggga cagacccgcg gcgagtatga agagcaaggc gccgaacgag
     1681 atgccgttca gcgcctgctg caccgcgact tcggggctca tgccggcttc gccgccatga
     1741 gcccaacacc tgctcgtcgc aggggagggg gcaggggccc ccgggcccga agggacgccc
     1801 ctgccacccc gggtacgacc ctacttccca gggtccgtca ctgccgcgat cttgtcggtg
     1861 atcgtgttca cgacctgccc gccctggacc ttgacctcgc ggatgtagat gtcgtggatg
     1921 acgttgtgcg tgtccttgtc gaatgagaag tcgccacgcg ggctcttgaa cttgacgccc
     1981 tcgagcgcgg cgatgaacgc gtccttgttc gaggtgtccc cattcgtctt ctgcagcgcg
     2041 aggtcgagcg cctgcatgcc gtcccacgcc tgcaccgcga agacatcggg cagcaggttg
     2101 tactccttct gataggcctc gacgaatgcc ttgttctccg cgttgtcgag ggtcacggcc
     2161 cagaacagcg acgtgacggc gccgacggcg agctccttca cttccttgag cacgtcctgc
     2221 tcggtgagga agcccgcgcc gttcatccgg tagccggccg ccggcatgcc gagctggttc
     2281 cacgccttga tgtagttgat cgcgtcggta ccggagaaga aggagtagat gtccttcgtc
     2341 ggctggttct tcagctgcgt cacgaccggg aggaagtccg cggtcgggag cggggtcttc
     2401 agcgtgccgg tgaccttgcc gccggccgcc tggaaggcgt tcgtgaagtc ggccgcggac
     2461 tcgttgccga agccgtagtc cgacacgaac gtgaaggctt ccttcacgcc cttcttcgtc
     2521 gcgaagtacg tgccgatggg gttcgagatc tgccacgacg agaacgacgt gcggaagatg
     2581 tacttgctct tgcacgcggg cttgcagtcg gtcgtcgtgc gcgtgagggc gttgccgccg
     2641 gcgttggtct cgatgaagac catcttcgcg gagtccaccg cgttgcggat cgcgtacgcg
     2701 atcggcgtcg cgacgatgcc catcatgacg tcgacctggt cctgctcgat gaacttctgc
     2761 gtcttctgcg cgccgacggc cggatcgttc gcctcgtcct cgtagatggg cggcgcgacg
     2821 gtgcgcccgc cgagcttgtt gcccttctgc ttgagataga ggtccgtcgc gcgcttcatc
     2881 gagttgccga gctcggcgta caccttcgtg aacggaagga gctggccgat cttgagcggc
     2941 ttggcggaga ccgtggcgga cgtggccgcc gcggccgacg tcgcgggcgc gccgctcgca
     3001 cccggtgtcg cggccggacc gcacgcggcc gcgagcgccg ccgcggagct tgcgacgagg
     3061 aacttgcgtc gtgtgagccg agacgaattt gacccgttca ctgcctacct ccccgggcgg
     3121 ccaggggccg ccgcgcgagc ctaggacgcg ctcgggacgc tgacaagcag gcgcggcgaa
     3181 gaagccgcgc tgtcgctcgc gctggccgcg agttgactag gactcgcgct cgaagagcgt
     3241 ggcgaggccc tgcccgacgc cgatgcacat ggtggcgatc gcgtaccgtc cggcccggcg
     3301 ctcgagctcg cgaagggcag taccggcaag ccgcgcgccg gacatcccga tgggatggcc
     3361 gacggcgatc gcgccgccgt tcggattcgt ccgatcctcc gggaggccga gttgccgcat
     3421 gcacgggagg acctgcgccg cgaacgcctc gttcagctcg atgacgtcgc actcgtccac
     3481 cgccacgcgc gtgcgctgaa cgagcttccg caccgccggg atggggccga gccccatgta
     3541 gtcaggatgc acgcccgccg aggcgctcgc gaccacgcgg ccgagcggac gcagcccgag
     3601 cgcggcggcc ttctcgcgcg agccgacgat gagcgccgcg gcgccgtcgt tgatcccgga
     3661 ggagtttccc gccgtgaccg tgccgtcctt cttgaacgcc ggtcgcagct tcgcgagctt
     3721 ctcgagcgtg acgtcgggac gcgggtgctc gtcgtcggag acgagctcct tgccgttgtg
     3781 caccggctgg atctcgccct tgaagaaccc cgcctccttc gccttcgcgt agcgctgctg
     3841 gctgcgaagc gcgtacgcat cctggtcctc gcgcgacacg tgctcctgga cgcacacgtt
     3901 ctccgcggtc tcaccgaggc cgatggggtg atagccgagg tcgatcatgc gcggattggt
     3961 catgcgccag ccgatcgtcg tgtcgtagat ggtgcgctcg ccgcgcggga acgcgtcgga
     4021 cggcttcagc atcacgagcg gtgcgcgggt catgctctcg accccgccgg cgacgacgat
     4081 gtcggcctcg cccgcgcgga tgcgacgcgc ggcgtcgttg acggcctcga gcccactgcc
     4141 gcagagacga ttcagcgtga cccccggcac tgtgtcgggc agacccgcga ggagcgccgc
     4201 catgcggccg atgttgcggt tgtcctcgcc ggcctggttg gtcgcgccga agatgacctc
     4261 gtccacgtcg gcgtcgccga tatcggtccg gtcgagcacc gcgcgcacca cgtgcgacgc
     4321 gaggtcgtcg ggccggatcg acgcgagcac accgccgtag cgcccgatcg gcgtgcgcac
     4381 atatcccagg atcacggcgt cgcgcggaga ctgactcatc ggctcacttc ccttcgaacg
     4441 acgcgcgccg cttctcggcg aatgccttga tcgcctcggc gtagtcacgg gtgcgaccgg
     4501 cacgatcctg cagctcgacc tcgagctcga ggaacgaacg gaggtcgcgg tcccatgcag
     4561 cgttcagcga ctccttgctg agcgcgtagg ccccggtggc accctgcgcg atcgtcatgg
     4621 cgtaggcctt ccattctcgg gcgaactctg tgtcagacca cacacgggtc accaggccga
     4681 tgcgcagcgc ttcctccgcg tcgacggcgt cccccgtgag gatcatgtcc atggcccgtc
     4741 cccacccgac gatgcgcggc aggaagtacg ccgcacccgc gtcgggcgcg aggccgacgc
     4801 ggctccacgc cg
//
LOCUS       DASTZR010000359         4272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1247245, whole genome shotgun sequence.
ACCESSION   DASTZR010000359 DASTZR010000000
VERSION     DASTZR010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247245"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VFV20_08025"
     CDS             complement(<1..256)
                     /locus_tag="VFV20_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288335.1"
                     /translation="MKNIPPEKLRYLDVMKERLHGSDDPALIRSDQRLVIEWLGESFG
                     RTTIRGVELVSDEHAEGTGYGRGVSPGHTFLAGFGFSHMTQ"
     gene            complement(253..1137)
                     /locus_tag="VFV20_08030"
     CDS             complement(253..1137)
                     /locus_tag="VFV20_08030"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5288336.1"
                     /translation="MRLVAFSRPPALVVASRRGFFADEGLEVSFTPTASSRAQLDALL
                     AGDHDVAHTAADNVVARVDAGADLRIVLVADRGVRHRLIGAPAVGTLGALAGRRLGVD
                     APESGHAILAYVLLAEAGVARGGCEIVAVGSTRERSAALLDGRIDAAMLNAPHDERAI
                     AAGCHVLADAAARFPRHPGLTVAATRAWSAAHPVELEAYCRALLRGARFAADPANRGA
                     VIADLAADQGLAAREAERVYERERQGDVPAIHEMADAVASVCEARRAAGLAHGPFDVQ
                     RYFDPSYVQAAEAAGGRR"
     gene            complement(1134..2855)
                     /locus_tag="VFV20_08035"
     CDS             complement(1134..2855)
                     /locus_tag="VFV20_08035"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU5288337.1"
                     /translation="MSPFRGLARVRVGADTLLLAFCGLFVAYLVLSPIAILILTSFRR
                     YKFDGNIGFVLTLENYIETYANPEIPQAVLNTVIYAGAGTLLAVVAGTALAWVVERTN
                     TPFRSMFTLAAAATFFVPGMLFALAWSALANPRIGTLNGILRGVFDVAEGPIDINSMG
                     GMIWVFGTHLYPVVFLIMSAAFRSMDPSLEEAAALSGAGTWRMLRTVTLAVSRPALIS
                     GALIMFVRGLESFDVPLILGLPAGVTTLTTEIFATASLHQPPELGISASLGVILLIVS
                     AVGVWFYRSQTAGALAFATITGKAYRPHRLDLGGMKWLTSAICMVFFFITLVLPLLAL
                     FWISLFPFVRQFSFAALQRASLNQYAYVLSYSTIVDAFRNSVINAVLVATLVVLLTSV
                     IAWIVLRSRLPARGALDALAFAPIGIPGTIMGVAILLVYLTLPIPLYGTIFIISVAHL
                     TLYLPYGMRLASDAIVRIHPQLEEQSALSGASWFSTYRRVLLPLLAPGLVAAWITIVA
                     ASFRELSAAIFLASPQSRFVSVMLYSTWNDGNATAAAALGIVVLFVVLLLALVGRTVG
                     NRFRIAE"
     gene            complement(2882..3976)
                     /locus_tag="VFV20_08040"
     CDS             complement(2882..3976)
                     /locus_tag="VFV20_08040"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5288338.1"
                     /translation="MFDARRLARTIAFLATMSLISAACGGGSPATTAPTNAATATAVA
                     TKPPTFDEAAETKKLYDAAVAAGEKEVNFYGSINEEEAKPLLDIWAKAFPLIKNNYIR
                     ASESALVSRILTEAQAGKYVVDTLSTTSAHLLVPAGLALNYLPPNAQLVDPDYKDKDG
                     AWIGIYANWNVIQVNTDKVKAGEIKTYEDIANPKWKGQVVVDDTDYEWYQGLIKVLGQ
                     QKADDLLKKIHSTVGVKVLDGHGTINDKVTAGEYAIALNNYLNQAERSKRLGGPTNWI
                     AVEPVVVQLGKIVTAKNAPHPNAAKLLTNFLISTDAQKYLTGRGRITTRTDVPNDPPN
                     LVTGLKKTSSPPLVDPELTALAKKFKELFK"
     gene            4069..>4272
                     /locus_tag="VFV20_08045"
     CDS             4069..>4272
                     /locus_tag="VFV20_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018658434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5288339.1"
                     /translation="MLTVEGLRKSFVTDRGDVRAVQHVSFTVEEGRFYTLLGPSGCGK
                     TTTLRCIAGLERPEAGRIQIAGRV"
BASE COUNT          673 a         1410 c         1560 g          629 t
ORIGIN      
        1 actgcgtcat gtggctgaag ccgaagccgg cgaggaaggt atggccgggg ctcacgcccc
       61 tcccgtagcc ggtgccctcg gcgtgctcgt ccgacacgag ctcgacgccg cggatggtgg
      121 tgcggccgaa cgactcgccg agccactcga tgacgagccg ctggtccgag cggatgaggg
      181 ccggatcgtc cgagccgtga agcctctcct tcatgacgtc caggtagcgc agcttctcgg
      241 gcgggatgtt cttcatcgcc ggccgccggc cgcctcggcc gcctggacgt acgacgggtc
      301 gaagtaccgc tggacgtcga acgggccgtg tgcgagaccg gcggcgcgcc gggcctcgca
      361 gaccgacgcg acggcatccg ccatctcgtg gatcgccggc acgtcgccct gccgctcgcg
      421 ttcgtagacg cgctccgcct cccgcgccgc gaggccctgg tcggccgcga gatcggcgat
      481 caccgcgccg cgattcgccg ggtcggccgc gaagcgcgcg ccgcgcagga gcgcccggca
      541 gtacgcctcg agctcgacgg ggtgcgcggc cgaccacgcg cgcgtggccg cgacggtgag
      601 cccggggtgg cgcggaaagc gcgccgccgc gtcggcgagc acgtggcatc ccgccgcgat
      661 cgcgcgctcg tcgtgcgggg cgttcagcat cgccgcgtcg atccggccgt cgagcagcgc
      721 cgcactgcgt tcgcgcgtcg agcccaccgc gacgatctcg cagccgccgc gcgccacgcc
      781 cgcctccgcg aggagcacgt acgcgaggat cgcgtggccg ctctcgggcg catcgacccc
      841 gaggcggcgt ccggcgagcg cgccgagcgt cccgaccgcg ggcgcgccga tgagccgatg
      901 ccgcacgccg cggtcggcga cgaggacgat gcggaggtcg gcgcccgcgt ccacgcgcgc
      961 gacgacgttg tcggccgccg tgtgcgcgac gtcgtgatcg cccgcgagca gcgcgtcgag
     1021 ctgcgcgcgc gagctcgccg tcggcgtgaa cgacacctcg aggccctcgt ccgcgaagaa
     1081 cccgcggcgc gacgccacga cgagcgccgg cggtcgcgag aacgcgacca gcctcattcg
     1141 gcgatgcgga agcggttgcc gacggtacgg ccgacgagcg cgaggaggag caccacgaag
     1201 agcacgacga tgccgagcgc ggctgccgcg gtggcgttgc cgtcgttcca ggtcgagtac
     1261 agcatcaccg agacgaagcg cgactgcggc gacgcgagga agatcgcggc ggagagctcg
     1321 cggaaggacg ccgcgacgat cgtgatccac gcggccacga gacccggtgc gagaagcggc
     1381 agcagcacgc ggcggtaggt gctgaaccag ctcgcgcccg acagcgcgga ctgctcctcg
     1441 agctgcggat ggatgcggac gatcgcgtcg ctcgcgagac gcatgccgta cggcagatac
     1501 agcgtgagat gggcgacgga gatgatgaag atcgtgccgt agagcgggat gggcagcgtc
     1561 aggtacacga gcaggatcgc gacgcccatg atcgtcccgg ggatcccgat cggggcgaac
     1621 gccagagcgt cgagcgcgcc gcgggccggc aggcgcgagc gcagcacgat ccacgcgatc
     1681 accgacgtga gcaggactac gagcgtcgcg acgagcaccg cgttgatgac ggagttgcgg
     1741 aacgcgtcga cgatggtgct gtagctcagg acgtaggcgt actggttgag cgacgcccgc
     1801 tgcagcgccg cgaaggagaa ctggcggacg aacgggaaca gggagatcca gaagagcgcg
     1861 aggagcggca gcaccagggt gatgaagaag aacaccatgc agatcgcgga ggtgagccac
     1921 ttcatgcccc cgaggtcgag gcggtgcggc cggtaggcct tgccggtgat ggtcgcgaac
     1981 gcgagcgcgc ccgcggtctg cgaccggtag aaccacacgc cgaccgcgct cacgatgagc
     2041 aggatcacgc cgagcgatgc cgagatcccg agctcgggcg gctgatgcag cgacgccgtc
     2101 gcgaagatct cggtggtgag ggtcgtcacg cccgccggca gcccgaggat gagcgggacg
     2161 tcgaaggact ccagcccgcg gacgaacatg atgagcgcgc ccgagatgag cgcggggcgg
     2221 gagaccgcga gcgtcaccgt ccgcagcatc cgccaggtcc ccgcgcccga gagcgcggcg
     2281 gcctcctcga gcgagggatc catgctgcgg aacgcggcgc tcatgatcag gaagacgacc
     2341 ggatagaggt gcgtcccgaa gacccagatc atgccgccca tgctgttgat gtcgatcggc
     2401 ccctcggcga cgtcgaagac gccccgcagg atgccgttca gcgtcccgat gcgggggttg
     2461 gcgagagcgc tccaggcgag cgcgaagagc atgcccggca cgaagaacgt cgccgccgcg
     2521 gcgagggtga acatcgaccg gaacggcgtg ttcgtccgct cgaccaccca cgcgagggcg
     2581 gtgcccgcga cgaccgccag cagggtgccg gcgccggcgt agatgacggt gttgaggacg
     2641 gcctgcggga tctccggatt ggcgtacgtc tcgatgtagt tctcgagcgt caggacgaag
     2701 ccgatgttcc cgtcgaactt gtaccggcgg aagctggtga ggatgaggat cgcgatcggg
     2761 ctcaggacga ggtaggcgac gaagagcccg cagaacgcga gcagcagcgt gtcggcaccg
     2821 acgcgcacgc gcgcgagccc gcggaacggg ctcacgcgcg gcgcgacggc accgccggct
     2881 gctacttgaa gagctccttg aacttcttcg cgagggccgt cagctcagga tccacgagcg
     2941 gcggcgacga ggtcttcttc agtcccgtca ccaggttcgg cgggtcgttc ggcacgtcgg
     3001 tccgcgtggt gatccggccg cggccggtca ggtacttctg cgcgtccgtg gagatcagga
     3061 agttcgtgag cagcttcgcg gcgttcggat gcggcgcgtt cttcgccgtc acgatcttgc
     3121 cgagctggac gaccacgggc tcgaccgcga tccagttggt cggtccaccg aggcgcttgc
     3181 tgcgctcggc ctggttcagg tagttgttga gcgcgatcgc gtattcgccc gcggtcacct
     3241 tgtcgttgat cgtcccgtgg ccgtcgagca ccttcacgcc caccgtggaa tggatcttct
     3301 tcaggaggtc atcggccttc tgctggccca ggaccttgat gaggccctgg taccactcgt
     3361 agtcggtgtc gtcgacgacg acctggccct tccacttcgg gttggcgatg tcctcgtacg
     3421 tcttgatctc gcccgccttc accttgtcgg tgttgacctg gatcacgttc cagttggcgt
     3481 agatgccgat ccacgcgccg tccttgtcct tgtagtcggg atcgacgagc tgcgcgttcg
     3541 gcggcaggta gttcagcgcg agtccggccg ggacgagcag gtgggcgctg gtcgtcgaga
     3601 gcgtgtcgac gacgtacttc ccggcctgcg cctcggtgag gattcggctg acgagcgcgc
     3661 tctcgctcgc gcggatgtag ttgttcttga tcagcgggaa ggccttggcc cagatgtcga
     3721 ggagcggctt cgcctcctcc tcgttgatcg agccgtagaa gttcacttcc ttctcgcccg
     3781 cggcgaccgc ggcgtcgtag agcttcttcg tctccgcggc ctcatcgaac gtcggcggct
     3841 tcgtggcgac cgcggtcgcc gtcgcggcgt tcgtcggcgc cgtcgtcgcc ggcgatccgc
     3901 ctccgcaggc ggcactgatg agggacatgg tggcgaggaa cgcgatggtc cgtgcgagtc
     3961 ggcgtgcgtc gaacattccc tctgaccctc ctactggcgc tctgcgcccg gctggagtat
     4021 agggattggt aataccattc cagcgaccgc gaacgaggag aggtcgggat gctcaccgtc
     4081 gagggcctgc gcaagagctt cgtgacggac cgcggcgatg tgcgtgccgt gcagcacgtg
     4141 tcgttcaccg tcgaggaggg gcgcttctac acgctcctcg gcccgtccgg ctgcgggaag
     4201 accaccaccc tgcggtgcat cgcgggtctc gagcgtccgg aagccggacg gatacagatc
     4261 gccggacgcg tc
//
LOCUS       DASTZR010000360         3103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1195103, whole genome shotgun sequence.
ACCESSION   DASTZR010000360 DASTZR010000000
VERSION     DASTZR010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195103"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..154)
                     /locus_tag="VFV20_08050"
     CDS             complement(<1..154)
                     /locus_tag="VFV20_08050"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU5288340.1"
                     /translation="MGEHTLRFQLNGSPREVRVSASARLIDAVRDGCGLTGTKEGCSV
                     GVCGACS"
     gene            complement(155..1678)
                     /locus_tag="VFV20_08055"
     CDS             complement(155..1678)
                     /locus_tag="VFV20_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEU5288341.1"
                     /translation="MFNLAAAMLDPHLAAGRGARTAYRYEGDTITYAEIADLVARTGV
                     GLRQLGIDLEDRVALLLPDSPQFVASFLGAMRIGAVPVTLSTLLSTDDYAYLLADCRA
                     RALVVHESVLAKVEPLLDCLPSLRHVVISGKAERSGAIPFAALTGGSAGDLPPVETTE
                     DDMAFWQYSSGTTGRPKGVVHLHGNAYRPADLHGRLVAGITADDRIFSVPKLFFSFGL
                     NNSSIFPLRHGASAVLLPDRPDPRLVLDTIARERPTILYHVPTGYAGLLAAAEAGAQA
                     DLSSVRLCVSAGEALPAPIFERWKARFGLEILDGIGSTEIGYICISNMPGRARPGTSG
                     QVIPGYEAKVLREDGTRAAVGEVGDLLVRGPSTAAFYWRKREATKHTFRGEWVFTGDR
                     YAVDAEGYFTYQGRSDDMIRVSGLWVSPIEVETALLRHPRVRECAVVARAEENGLVRP
                     CAYVVPVGDATDDLASELVAFASEALSAYKRPKWVRFLAELPKTSTGKVQRFKLRDR"
     gene            complement(1693..2415)
                     /locus_tag="VFV20_08060"
     CDS             complement(1693..2415)
                     /locus_tag="VFV20_08060"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HEU5288342.1"
                     /translation="MAQFSDRIEPKDFTRMPDEYRDLLVRLLTIQADCEIGGPHIYVD
                     HWTLRAPTVDDQWRVARIAAEEIDHCRKMLRLLRLVDVDRSDILERPRSKREVDAFKE
                     DMPTWADFAAFGFLIDKVGEYQLDEMVGSSYLPMDDALPVILREEKGHVAYGEQQLEK
                     LVRSGDAGRASAQAAVDKWYVVGLDMFGRSGSARTERYIEWGLKKRTNEEARRQYIAE
                     MEPKIQAIGLVVPDRLQGRKYL"
     gene            complement(2555..>3103)
                     /locus_tag="VFV20_08065"
     CDS             complement(2555..>3103)
                     /locus_tag="VFV20_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5288343.1"
                     /translation="ELRAVARDVPVIVGALGPQMLRLAGREAQGAALNWCTPEQVAWS
                     RERAGPNAGMVDYIRVCVDDDVPAARLALAEQILAYALLVRPSGASGYRTHFERMGFA
                     DELAFLSSRRAAGASDAEVARVMPERLLETFGYAGRGDGAQEWFARMSRGLDVAIVRI
                     LTPRPGDPRPVEAAMRAFAPSH"
BASE COUNT          476 a         1103 c         1088 g          436 t
ORIGIN      
        1 cgctgcacgc gccgcacacc ccgaccgagc agccctcctt cgtcccggtg aggccgcagc
       61 cgtcgcgcac ggcgtcgatg aggcgcgcgc ttgccgaaac gcgcacctct cgcggtgaac
      121 cgttgagctg aaagcgcagc gtgtgctcgc ccatctacct atccctcaac ttgaagcgct
      181 gcaccttccc cgtcgaggtc ttcggcagct ccgccaggaa tctcacccac ttcggacgtt
      241 tgtacgccga cagcgcctcg gacgcgaacg cgaccagttc ggacgcgaga tcgtcggtcg
      301 catcgcccac cgggacgacg tacgcgcacg gccgcaccag gccgttctcc tcggcgcgcg
      361 ccaccaccgc gcactcgcgc acccgcggat gccgcaggag cgcggtctcg acctcgatcg
      421 gcgacaccca gagcccgctc acgcggatca tgtcgtcgga ccggccttgg taggtgaagt
      481 acccctcggc gtccaccgcg tagcggtcgc cggtgaagac ccattcgccg cggaaggtgt
      541 gcttggtcgc ctcgcgtttg cgccagtaga acgcggcggt cgatggaccg cgcaccagca
      601 ggtcacccac ctcgcccacg gccgcgcgcg tgccatcctc gcgcagcacc ttcgcctcgt
      661 agccggggat cacctggccc gaggtccccg ggcgcgcgcg gccgggcatg ttcgagatgc
      721 agatgtagcc gatctcggtg gacccgatgc cgtcgaggat ctcgagcccg aagcgcgcct
      781 tccagcgctc gaagatgggc gcgggcagcg cctcgccggc ggagacgcac aggcggaccg
      841 acgagagatc cgcttgcgcg cccgcctccg cggcggcgag gagtcccgcg tatcccgtcg
      901 gcacgtggta caggatggtc ggccgctcgc gcgcgatggt gtcaaggacg agtctcgggt
      961 cgggccgatc cggcaggagc accgcgctcg cgccgtgccg cagcgggaag atcgacgagt
     1021 tgttcagccc gaacgagaag aacagcttgg gcacgctgaa gatgcggtcg tcggcggtga
     1081 tgccggcgac gagccgaccg tgcaggtcgg ccgggcggta ggcgttcccg tgcagatgca
     1141 cgacgccctt cggccgcccg gtcgtgccgc tggagtactg ccagaaggcc atgtcgtcct
     1201 cggtggtctc caccggcgga aggtcgccgg cggacccacc cgtgagcgcc gcgaatggga
     1261 tggcaccgct gcgctcggcc ttgccgctga tgaccacgtg gcgcagcgat ggaaggcagt
     1321 cgaggagcgg ctcgaccttc gcgaggacgc tctcgtgcac gacgagcgcg cgcgcgcggc
     1381 agtcggcgag caggtacgcg taatcgtcgg tcgaaaggag cgtcgacagc gtcaccggca
     1441 cggcaccgat ccgcatcgcg ccgaggaacg acgccacgaa ctgcggcgag tcgggcagga
     1501 ggagcgcgac acggtcttcc aggtcgatgc ccagctgccg caggccgacg ccggtgcgcg
     1561 cgacgaggtc ggcgatctcg gcgtaggtga tcgtgtcgcc ctcgtagcgg tacgccgtgc
     1621 gcgcgccacg gccggcggcc aggtgcggat cgagcatcgc ggcggcgaga ttgaacacgg
     1681 tgcccgccgc ggctagaggt acttcctacc ctggagccgg tcggggacga cgaggccgat
     1741 cgcctggatc ttcggctcca tctcggcgat gtactggcgc cgcgcctcct cgttggtccg
     1801 cttcttcagg ccccactcga tgtagcgctc ggtgcgcgcg gagcccgagc ggccgaacat
     1861 gtcgaggccg acgacgtacc acttgtcgac cgccgcctgc gcggaggccc tgcccgcgtc
     1921 gccgctccgc acgagcttct cgagctgctg ctcgccgtat gcgacgtgcc ccttctcctc
     1981 gcggaggatg accggaagcg cgtcgtccat cggcagatac gacgacccca ccatctcgtc
     2041 gagctggtac tcgcccacct tgtcgatgag gaagccgaac gcggcgaagt ccgcccaggt
     2101 cggcatgtcc tccttgaagg cgtcgacctc gcgcttgctg cgcggccgct cgaggatgtc
     2161 ggagcggtcg acgtcgacga gccgcaggag ccggagcatc ttgcggcagt gatcgatctc
     2221 ctccgccgcg atgcgcgcga cgcgccactg gtcatcgacg gtgggagcgc gaagcgtcca
     2281 gtggtcgacg tagatgtgcg gtccaccgat ctcgcagtcg gcctggatgg tgaggaggcg
     2341 gacgagcaga tcgcggtact catccggcat cctggtgaag tccttcggct cgatgcggtc
     2401 gctgaactgc gccatccgtt cgtccctccg gcggcgagaa tgagtagacc ttagcaccga
     2461 gcgacgagcg gctttgccgc gaggagcgag tccgacagag cggagcgagg tggtcgaggc
     2521 gacgccgcga agcaggcgga gccgagcgcg aaaactagtg cgacggggcg aacgctcgca
     2581 tggcggcctc gacgggacgc ggatcgccgg gccgcggcgt gaggatgcgc acgatcgcca
     2641 cgtccagccc gcgcgacatc cgggcgaacc attcctgagc gccgtcgccg cggccggcgt
     2701 agccgaaggt ctcgaggagc cgctcgggca tgacgcgcgc gacctccgcg tcgctcgcgc
     2761 cggccgcccg tcgtgacgac aggaacgcga gctcgtcggc gaagcccatg cgctcgaagt
     2821 gggtgcggta gccgctcgcg cccgacggcc gcaccagcag cgcgtacgcg aggatctgct
     2881 cggcgagcgc gagccgcgcc gccggaacgt cgtcgtcgac gcacacgcgg atgtagtcga
     2941 ccatccccgc gttcggaccc gcacgctcgc ggctccacgc gacctgctcc ggcgtgcacc
     3001 agttgagcgc cgcgccctgc gcctcccgac cggcaaggcg cagcatctgc gggccgagcg
     3061 cgccgacgat caccgggacg tcccgtgcca ccgcgcgcag ctc
//
LOCUS       DASTZR010000361         5945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1064660, whole genome shotgun sequence.
ACCESSION   DASTZR010000361 DASTZR010000000
VERSION     DASTZR010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5945
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064660"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VFV20_08070"
     CDS             <1..357
                     /locus_tag="VFV20_08070"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEU5288344.1"
                     /translation="YGGQGNANPWIDELCEKLPQSYGALINTRLARTLGVRDGEWIWL
                     ESPVRKTRVRAHVTECVHPEIIGIAGHAGHWAKGKARTRGKGVSFNSLLPYDLAHLDA
                     FTSAVDMCAPLTVRKA"
     gene            354..1013
                     /locus_tag="VFV20_08075"
     CDS             354..1013
                     /locus_tag="VFV20_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU5288345.1"
                     /translation="MTRLGFVVDLERCVGCQGCVLACKLEHGTPRGVHWMRVLEREVG
                     TYPNARRTFTPVRCNHCANAPCIAACPTGAIVKRPDGIVLVNDQVCVGTMACLTACPY
                     YIPVRYDGDGAYYEDGPTPFEEVKQGKFRVGTAQKCTFCDHRIDQGRPPACVEACPAD
                     ALNFGDLDDPRSRINELLNTRRHFRPREELGTDPSVYYLVGRGGGTVGGMDPGAAEAG
                     E"
     gene            1010..2020
                     /gene="nrfD"
                     /locus_tag="VFV20_08080"
     CDS             1010..2020
                     /gene="nrfD"
                     /locus_tag="VFV20_08080"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEU5288346.1"
                     /translation="MSAKPRSLARALERAWSGWRPERRPHGNVGRPFDLRYPVQQHWG
                     AKVAFYLYLGGTGAGLVFLEVLFRWQGDLDGRTAAIGMWIGLALAVLSALAIFDHLGP
                     VSRWSAYFAFRNLRRSWIARGVTMLFALLLLRLLLALPTLPGAEGLPWAEGTAAGDVL
                     RVAVLLFAFAFMAYTGLVISSWNAIAFWNTPLVPVLFVASSLLGGVMGLAAVGWVAGG
                     PAGMHTVGSIAFPYALQLLAADAALLAVYVYGMSTATRPARVSVSMLLRGALRRRFLG
                     GAVALGLVLPALVLVPQATGWLTCSVDTAWIFLAAIAGVQAGGYFLRDAVLRAGVYGP
                     PV"
     gene            2023..2895
                     /locus_tag="VFV20_08085"
     CDS             2023..2895
                     /locus_tag="VFV20_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018568960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5288347.1"
                     /translation="MRIGIGLPTRASDMAPELLFTWAERAERGPFSSVAVSDRVVYDS
                     QEPLVALAAVAGVTRRVRLVTSIVLAPTRETTLLARQSATLDALSGGRLTLGVAVGAR
                     EDDYTATGTRFHRRGRRLDAQLPELRKIWSGAPIATGGVIGPAPHRLGGPELLIGGYL
                     PVVARRIAAWGDGFLQPGGGDPGAMAALWSDIEKAWAAAGRGGRPRWVSGSYFALGPD
                     ADAAADAYIKATYAFDPKVAERRRKTLPTTPDAVRAAIARQADMGVEEFILRPCTADI
                     EHLDRLAELVVAVA"
     gene            complement(2930..4399)
                     /locus_tag="VFV20_08090"
     CDS             complement(2930..4399)
                     /locus_tag="VFV20_08090"
                     /inference="COORDINATES: protein motif:HMM:NF018032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HEU5288348.1"
                     /translation="MTHSHTHQHGHAHDAGDGNVASERLRLRTVGIDIGTTTSHLIFS
                     ELVLERQGIRLSSAYTVVERNVIHPSDVSLTPYASARRIDTDALAAFIARSYEAAGWT
                     PQTVDTGAVITTGEAARKENAAAIVGLFSGQAGRFVCATAGHHLEALLAAHGSGAVAR
                     SRSDETPLVLNVDIGGGTTKLAVCRHGHVDETAAIDVGARLVSWNGDGTVRAVTSAGQ
                     RVAAAAGVRVSAGDRPDRDVLGTLAEKIAEIALRVPEGQPMGDDELWLTEPLHTRGPF
                     PALVFSGGVGEYVNGNETREFGDLGKLVGAAIAKRLGGHEVLRAVESIRATCIGASQY
                     TIQVSGDTLFLSDSAILPLRDLAAIAIRPAAASATDIARTVRAGIARLDREDGAFAIA
                     VRWQHGPAYAALRELCTGIGDAVRDVLPADRPLVVVLDADVAGIVGQVLQDEVGVRSP
                     IVCVDQIQLSDLDFIDIGELVPEKAVVPVVVKSLVFSAR"
     gene            complement(4409..5524)
                     /locus_tag="VFV20_08095"
     CDS             complement(4409..5524)
                     /locus_tag="VFV20_08095"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5288349.1"
                     /translation="MSETTPATAKEQQRPSFWGRGAYEIFIESEGIPIHTGTDVPDLR
                     TAQVGKWARLDAKGAYLRLYGAEDTDNAYILELDPGKSSAPEKHLFEEYFFVLTGRGT
                     CEVWNEGEKPTTFEWQEGSLFSVPLNTLHRMHNGSGSLAARLLAVTTMPIMMNLLHNT
                     DFMFGCPYVFTDRYDGRKEYFDGAGTLYTGRVWETNFVADIRRFQLKDWLERGAGGKN
                     IRFEFANNTMVSHVSEFPVGTYKKCHRHGPGYHVILLGGEGYSYMWPGDANLPSDKWT
                     EVKWKTGTLFVPPGRWWHQHFNTGNEPARYLAIRWGGNKWKLAEYLDNQGVDKDVNEG
                     GNQIEYEDQPPTIHRTYLERCAANGVQVKMDAFKVRV"
     gene            5653..>5945
                     /locus_tag="VFV20_08100"
     CDS             5653..>5945
                     /locus_tag="VFV20_08100"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEU5288350.1"
                     /translation="MFQDVARTVRVAARPDAAWAVLLDVQRVASWLKVVRDVKELEPL
                     RRYTTVLEDRVGPFAMRADLAIDVTADEAARRLHVSGSGEDRQVASRISATLDL"
BASE COUNT          797 a         2100 c         2114 g          934 t
ORIGIN      
        1 tacggcggcc agggcaacgc gaacccgtgg atcgacgagc tctgcgagaa gctcccgcag
       61 tcgtacggcg cgctcatcaa cacgcgcctc gcgcgcacgc tgggcgtgcg cgacggcgaa
      121 tggatctggc tcgagtcgcc ggtgcggaag acgcgggtcc gcgcgcacgt gaccgaatgc
      181 gtgcatcccg agatcatcgg catcgccggc cacgccggcc actgggcgaa gggcaaggcg
      241 cgcacgcgcg gcaagggcgt gagcttcaac tcgctgctcc cgtacgacct cgcgcacctc
      301 gatgcgttca cgtccgccgt cgacatgtgc gcgccgctca cggtgcgcaa ggcatgacgc
      361 ggctcggctt cgtcgtcgac ctcgagcgct gcgtggggtg ccagggatgc gtgctcgcgt
      421 gcaagctcga gcacggcacg ccgcgcggcg tgcactggat gcgcgtgctc gagcgcgagg
      481 tcggcacgta cccgaacgcg cgacgcacct tcacgcccgt gcgctgcaac cactgcgcga
      541 acgcgccgtg catcgcggcg tgcccgacgg gcgcgatcgt caagcggccc gacgggatcg
      601 tgctcgtgaa cgaccaggtc tgtgtcggca cgatggcgtg cctcacggcg tgcccgtact
      661 acatcccggt ccgctacgac ggcgacggcg cgtactacga ggacggaccc acgccgttcg
      721 aggaagtgaa gcagggcaag ttccgcgtgg gcacggcgca gaaatgcacg ttctgcgatc
      781 accgcatcga ccagggccgt cccccggcgt gcgtcgaggc gtgtcccgcc gacgcgctga
      841 acttcggaga cctggacgac ccgaggagcc gcatcaacga gctgctcaac acacgacggc
      901 acttccggcc gcgcgaggag ctcggcaccg atccgtcggt gtactacctc gtcggccggg
      961 gtggggggac cgtgggcggg atggatccgg gcgccgccga ggccggcgag tgagcgcgaa
     1021 gccgcgatcg ctcgcgcggg cgctcgagcg tgcctggagc ggatggcggc ccgagcgccg
     1081 gccgcacggc aacgtcggtc gtccattcga cctgcgctat ccggtgcagc agcactgggg
     1141 cgccaaggtc gcgttctacc tgtacctcgg cggcaccggc gccggcctcg tcttcctcga
     1201 ggtgctcttc cgctggcagg gtgacctcga cggtcggacc gcggcgatcg gcatgtggat
     1261 cggcctcgcg ctcgccgtcc tctccgcgct cgcgatcttc gaccacctcg gccccgtctc
     1321 gcgctggagc gcgtacttcg ccttccgcaa cctgcgccgg agttggatcg cgcgcggcgt
     1381 gacgatgctc ttcgcgctcc tgctgctccg cctgctgctc gcgctgccga cgctgcccgg
     1441 ggcggaggga ctgccgtggg ccgagggaac ggcggcgggc gatgtgctgc gcgtcgcggt
     1501 cctgctgttc gcgttcgcgt tcatggccta caccgggctc gtcatctcgt cgtggaacgc
     1561 catcgcgttc tggaacacgc cgctcgtgcc ggtgctgttc gtcgcgtcct cgctcctcgg
     1621 cggggtgatg gggctcgccg cggtcggctg ggtcgccggc gggccggcgg ggatgcacac
     1681 cgtcggcagc atcgcgttcc cgtacgcgct gcagctgctc gccgcggacg cggcgctgct
     1741 cgcggtgtac gtctacggca tgagcaccgc gacgcggccg gcgcgcgtga gcgtgtcgat
     1801 gctgctgcgc ggtgcgctgc gccggcggtt cctcggcggt gctgtcgcgc tcgggctcgt
     1861 cctgcccgcg ctcgtcctcg tcccgcaggc gacgggctgg ctcacctgca gcgtggacac
     1921 ggcgtggatc ttcctcgcgg cgatcgccgg agtacaggcc ggcggctact tcctgcgcga
     1981 cgcggtgctg cgcgccgggg tgtacggtcc gccggtgtga gcatgcgcat cggcatcggc
     2041 ctcccgacac gcgcgtccga tatggcgccc gagctgctgt tcacctgggc cgagcgcgcg
     2101 gagcgcgggc ctttctcgag cgtcgcggtg agcgaccgcg tggtctacga ctcgcaggag
     2161 ccgctcgtcg cgctcgccgc cgtcgcgggt gtgaccaggc gcgtgcgcct ggtgacgagc
     2221 atcgtcctcg cgcccacgcg ggagacgacg ctgctcgcgc gtcagtccgc gaccctcgac
     2281 gcgctctcgg gcggccgcct cacgctcggc gtcgccgtgg gcgcgcgcga ggatgactac
     2341 accgcgaccg gtacgcgctt ccaccgccgc ggacggcgcc tcgacgcgca gctgccggag
     2401 ctccggaaga tctggtccgg cgcgccgatc gcgaccggcg gggtcatcgg ccccgcgccg
     2461 caccggctcg gcggcccaga gctgctcatc ggcggctacc tgccggtcgt cgcgcggcgc
     2521 atcgccgcgt ggggcgacgg cttcctgcag ccgggcggcg gcgaccccgg cgcgatggcg
     2581 gcgctctggt ccgacatcga gaaggcgtgg gccgcggccg gccgcggcgg gcggccgcgc
     2641 tgggtgagcg gcagctactt cgcgctcggg cccgatgccg acgcggccgc cgacgcgtac
     2701 atcaaggcca cgtacgcgtt cgacccgaag gtcgcggagc ggcgccgcaa gacgctcccg
     2761 acgacgcccg acgccgtacg cgccgcgatc gcgcggcagg cggacatggg tgtcgaggag
     2821 ttcatcctgc ggccgtgcac cgccgacatc gagcatctcg atcgcctcgc tgagctcgtc
     2881 gtcgcggtcg cctgatccag cgtcagctcg gctcgcggcg tcccgctatt cagcgcgccg
     2941 agaacaccag actcttcacc accacgggca cgacggcctt ctcgggaacg agctcgccga
     3001 tgtcgatgaa atcgaggtcc gagagctgga tctggtccac gcacacgatc ggcgaacgca
     3061 cgccgacctc gtcctggagc acctgaccga cgatcccggc gacgtccgcg tcgagcacga
     3121 cgacgagcgg acggtctgcg ggcagcacgt cgcgcacggc gtcgccgatg ccggtgcaca
     3181 gctcgcgcaa cgcggcgtag gcgggaccgt gctgccaccg cacggcgatg gcgaacgcgc
     3241 catcctcgcg gtccagccgc gcgatccccg cgcggacggt ccgcgcgatg tccgtcgcgc
     3301 tcgcggcggc cggccggatc gcgatcgccg cgaggtcgcg gagcggcagg atcgccgaat
     3361 cgctgaggaa cagcgtgtcg ccgctcacct ggatcgtgta ctgcgacgcg ccgatgcacg
     3421 tggcgcggat gctctccacc gcgcgcagca cctcgtgccc gccaaggcgc ttggcgatcg
     3481 ccgcgccgac gagcttgccg agatcgccga actcgcgcgt ctcgttcccg ttgacgtact
     3541 cgcccacgcc gccggagaac accagggcgg gaaagggtcc gcgcgtgtgc agcggctcgg
     3601 tgagccacag ctcgtcgtcg cccatcggtt gcccttcagg gacacgcagc gcgatctcgg
     3661 cgatcttctc cgccagcgtc ccgaggacgt cccgatccgg tctgtcgcca gcgctcacgc
     3721 ggacgcccgc ggcggccgcg acacgctgac cggcgctcgt gaccgcgcgc acggtgccgt
     3781 cgccgttcca tgagaccagc cgcgccccga cgtcgatcgc ggcggtctcg tcgacgtggc
     3841 catgccggca gaccgcgagc ttcgtcgtgc cgccgccgat gtcgacgttg agcacgagcg
     3901 gtgtctcgtc cgaccgcgag cgcgcgaccg cacccgagcc gtgcgccgcg aggagggcct
     3961 cgaggtgatg gcccgccgtc gcacagacga aacggccggc ctggccgctg aagaggccga
     4021 cgatcgcggc ggcgttctcc ttgcgcgccg cctccccggt cgtgatgacc gcgcccgtgt
     4081 cgaccgtctg cggcgtccag ccggcggcct cgtaggagcg cgcgatgaag gccgcgagcg
     4141 catccgtatc gatccgccgc gcggaggcgt atggcgtgag cgagacgtcc gacgggtgaa
     4201 tgacattgcg ctcgacgacc gtgtacgcgc tcgagaggcg gatgccctgc cgctcgagca
     4261 cgagctccga gaagatcagg tgcgaggtcg tcgtgccgat gtcgatgccg accgtacgca
     4321 gccgaagccg ctcgctggcg acgttcccgt cgccagcgtc atgggcgtgc ccgtgctgat
     4381 gcgtgtgcga gtgggtcagc ggccggttct agacgcggac cttgaacgcg tccatcttca
     4441 cctgcacccc gttcgccgcg cagcgctcga ggtaggtgcg gtggatcgtc ggcggctggt
     4501 cctcgtactc gatctggttc ccgccctcgt tcacgtcctt gtcgacgccc tggttgtcca
     4561 ggtactccgc gagcttccac ttgttcccgc cccaccggat ggcgaggtag cgtgcgggct
     4621 cgttgccggt gttgaagtgc tggtgccacc agcggcccgg aggcacgaac agcgtgccgg
     4681 tcttccactt gacctcggtc catttgtcac tcgggaggtt cgcgtcgccc ggccacatgt
     4741 acgagtagcc ctcgccgccc aggaggatga cgtggtagcc aggcccgtgg cggtggcact
     4801 tcttgtacgt gccgaccggg aactcggaga cgtgcgacac catcgtgttg ttcgcgaact
     4861 cgaaccggat gttcttcccg ccggcgccgc gctcgagcca atccttcagc tggaagcggc
     4921 ggatgtcggc gacgaagttc gtctcccaca cgcgacctgt gtacagggtg ccggccccgt
     4981 cgaagtactc cttgcgtccg tcgtagcggt cggtgaacac gtaggggcag ccgaacatga
     5041 agtcggtgtt gtggaggaga ttcatcatga tcggcatcgt cgtcacggcg agcagccgtg
     5101 cggcgagcga gcccgatccg ttgtgcatgc ggtggagcgt gttcagcggg acggagaaga
     5161 ggctgccctc ctgccactcg aaggtcgtgg gcttctcgcc ctcgttccag acctcgcacg
     5221 tgccgcggcc ggtgaggacg aagaagtact cctcgaagag gtgcttctcc ggcgcgctcg
     5281 acttgcccgg gtcgagctcg aggatgtagg cgttgtcggt gtcctcggcg ccgtagagcc
     5341 ggaggtacgc gcccttcgcg tcgagccgcg cccacttgcc gacctgcgcg gtgcgcaggt
     5401 ccgggacgtc cgtaccggtg tggatcggga tgccctcgga ctcgatgaag atctcgtagg
     5461 cgccgcgtcc ccagaaggaa ggacgctgct gctccttggc tgtcgcgggc gtcgtctcac
     5521 tcatgatcca cacactccat ttaattagag cgaagcgtcg gggcgagacc tcaatcgtac
     5581 gcgagacgag cggatttgta tctcctttgg gagcgatggg atactgcgct cggacccccc
     5641 gaggaggctt tcgtgttcca ggatgtcgcg cgcaccgttc gcgtggccgc acgccccgac
     5701 gccgcgtggg cggtcttact cgacgtccag cgcgtcgcgt cgtggctcaa ggtcgtgcgc
     5761 gacgtgaagg agctcgagcc gcttcgccgc tacacgacgg tgctcgagga tcgcgtgggc
     5821 ccgttcgcga tgcgcgccga cctcgcgatc gacgtgaccg ccgacgaggc cgcgcgccgg
     5881 ctccatgtga gcggctcggg cgaggaccgg caggtcgcat cccgcatctc ggccacgctc
     5941 gacct
//
LOCUS       DASTZR010000362         2254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_801801, whole genome shotgun sequence.
ACCESSION   DASTZR010000362 DASTZR010000000
VERSION     DASTZR010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801801"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VFV20_08105"
     CDS             complement(<1..468)
                     /locus_tag="VFV20_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010899251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288351.1"
                     /translation="MPLTNGDIAELLSREAERHTEHGQRALRRAARAALWQWTDEASA
                     LREQGEPLTRLVLVGPWIAHLIGRWFEDAVDPPERPPLRADFLTLSEVRATLALHPEW
                     VRAYRGDLQVHTTWSDGAGTVAEMAELAAARGYEYVGITDHSKGLPIARGKDES"
     gene            complement(470..751)
                     /locus_tag="VFV20_08110"
     CDS             complement(470..751)
                     /locus_tag="VFV20_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288352.1"
                     /translation="MDWNLVDAGRLQGMRCPSCHGELTGGLMRQVEWTAQRCVVVVAC
                     PACTAECLAILETRPPRLAAVPPIDVDDVRRAHDLLGSRDWRVEQLFAA"
     gene            complement(950..2029)
                     /locus_tag="VFV20_08115"
     CDS             complement(950..2029)
                     /locus_tag="VFV20_08115"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEU5288353.1"
                     /translation="MLDVASAIGGAQAQVMSAAELQLAARSRSTVEDVRAALWTDRTL
                     VKTWLMRGTLHLIPAADLPLYTAALRRIIWTDAWFKWLRMTERELDSLVAAMGDELSD
                     QPMTKEELIARVGRGRPAHVREALSESWGSLLKPAARRGLLAFGPNRGTKVTLVNPRQ
                     WLPKWREIDTDDAIVEIARRYLRAYGPASKRDFRRWFRPSIRRGRGDPWPALSAELVD
                     VDVDGTRLQLLAKDRRALTSAARTPSVQLLPLFDPYLMGHSSRDHLFDKIHRWKVSRV
                     AGWISAVVLVDGRVEGTWTHAREGDTLVVNVAPFAALSARTRREIGTRADDLASALGL
                     SGAAVRQASSAKRDTRRDGSVQRLR"
BASE COUNT          328 a          808 c          796 g          322 t
ORIGIN      
        1 agactcgtcc ttcccacgcg cgatgggcag tcccttggag tggtctgtga tgccgacgta
       61 ttcgtagcca cgcgccgccg ccagctccgc catctccgcg acggtgccgg cgccgtccga
      121 ccacgtcgtg tggacctgga gatcaccgcg ataggcgcgg acccactccg gatgcagggc
      181 gagggtcgcg cgcacctccg agagcgtgag gaagtccgcc cgcaggggcg gccgctccgg
      241 cggatcgacc gcgtcctcga accaccgccc gatgaggtgg gcgatccagg gaccgacgag
      301 cacgagacgt gtcagcggct caccctgctc gcgcagcgcg gatgcctcgt cggtccactg
      361 ccacagcgcg gcccgcgccg cgcgtctgag cgcgcgctgc ccgtgctcgg tgtgccgctc
      421 ggcttcgcgc gacagcagct cggcgatgtc gccgttcgtc agcggcacgt cacgccgcga
      481 agagctgctc gacccgccag tcgcgcgatc cgaggaggtc gtgcgcgcgg cgcacgtcgt
      541 ccacgtcgat gggtgggacc gccgcgagcc gcggggggcg cgtctccagg atcgccaggc
      601 attccgcggt gcacgccggg catgccacga cgacgacgca gcgctgcgcg gtccactcga
      661 cctggcgcat gagcccgccg gtcagctcgc cgtggcacga ggggcagcgc atcccctgca
      721 ggcgcccggc atcgacgagg ttccagtcca tgcgtgcgtg acgggcagac aggatgccag
      781 ccgcgcccga gcgaccgggg ccatttgccg ccgcgcgacg cgcgccgagc gcgcgctcgc
      841 ttcctcgtcc tactcgcgga tcgcggccat tcgtctgcct gattcgctta cagccgaagt
      901 ctggtccgcc ggatcgtgcg cagaagtcgt gaatagtcgg cgttcggggt caccgcagcc
      961 gctgaacgga cccatcgcgc cgagtgtccc gtttcgctga cgatgcctga cgcaccgccg
     1021 cgccggacag acccagcgcg ctcgcgaggt cgtccgctcg cgtgccgatc tcgcgccgcg
     1081 tacgcgcgga gagcgcggcg aacggggcca cgttgacgac gagcgtgtcg ccctctcgcg
     1141 cgtgcgtcca cgtgccctcg acacgtccgt ccaccaggac gaccgccgag atccagcctg
     1201 cgacgcggct caccttccag cggtggatct tgtcgaagag gtggtcacgc gacgagtggc
     1261 ccatgagata cggatcgaag agcgggagca gctgcaccga cggcgtccga gccgcgctgg
     1321 tcagggcgcg gcggtccttc gcgaggagct gcaggcgcgt gccatctacg tcgacatcga
     1381 cgagctcggc cgatagcgcg ggccacggat cgccgcgccc tcgtcggatc gacgggcgga
     1441 accagcggcg gaaatcgcgc ttggaggccg ggccgtacgc gcgcaggtac cggcgcgcga
     1501 tctcgacgat cgcatcgtcg gtgtcgatct cccgccactt cgggagccat tgccgcgggt
     1561 tcacgagcgt gaccttcgtc ccacgattcg gtccgaatgc gagcaggcct cgccgtgccg
     1621 cgggcttcag caggctgccc cagctctcgg acagcgcttc gcggacgtgc gccggacgtc
     1681 cacgcccgac acgcgcgatg agctcctcct tcgtcatcgg ctgatcggag agctcgtcac
     1741 ccatcgccgc gacgagcgag tcgagctctc gctcggtcat gcgcagccac ttgaaccacg
     1801 catccgtcca gatgatcctg cgaagcgcgg cggtgtagag cgggaggtcc gccgccggga
     1861 tcaggtggag cgtcccgcgc atgagccagg tctttacgag cgtgcgatcg gtccacagag
     1921 cggcgcgcac gtcctccacc gtgcttcggg agcgagccgc gagctggagc tcggccgccg
     1981 acatcacctg ggcctgcgca ccgccgatgg cgctcgcgac gtcgagcaac gcgctccgcg
     2041 gcgcgcggcg ggtgaggtga tgccgcgcga gacggaacgc atgtacctgg gcccaggtga
     2101 ccgacggggt gctcagcggg aacgctcgag cgtgatgatc gggactgccg ccgcttcacg
     2161 cgccgttcgt acgcggcgaa gaacggcatg gcgctcgggg ccgtcatgcc acccaggcgg
     2221 ggcgccgctc gctggctgac gcattactgc gaga
//
LOCUS       DASTZR010000363         3877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_20422, whole genome shotgun sequence.
ACCESSION   DASTZR010000363 DASTZR010000000
VERSION     DASTZR010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20422"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VFV20_08120"
     CDS             <1..741
                     /locus_tag="VFV20_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEU5288354.1"
                     /translation="RRLREVGMRIEVDDFGTGYSSLGRLVDLPIDALKVDRRFVSAMT
                     RDHRSEAIVRACIALAHDLGLEVVAEGVEDRETWELLRDLGCDTAQGYYIGAAMPLDD
                     LGRWLGAWQRRVPAAVAKRERGTPIAGRQVLVVDDEPAIVAMIRDVLEDAGFEVVTAA
                     NGVEALRAVERGEPAVVLLDMQMPILDGAGFARLLRERGMRVPIVVMTAGSSAERWAR
                     ELPADAHLAKPFDIEGLLGVARRYAGAN"
     gene            complement(757..1590)
                     /locus_tag="VFV20_08125"
     CDS             complement(757..1590)
                     /locus_tag="VFV20_08125"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5288355.1"
                     /translation="MTATARPAAGIARHPDRLALLRAVCVREWREALGNKLLVGMTLL
                     PPAVILMAGIVAVGAAAANPPSERDVQALYAAAPAAQGLDPKEAVQGFIATYFLILFM
                     LIPTVVPLTMAIYSIIGEKSARTLEPLLAAPVGVGELLFAKSLASTVPSVIVTWIAYA
                     IYLASVSGLGSQAAVNAVTAPRWILAIIVMVPLLTLLSVNLGILISTRVNDVRVAQQI
                     GGLVVVPVVGVGIAQVTGRVVINNEAFVQFSLFLIVIDAAVFLLARLAFQRENILSRW
                     R"
     gene            complement(1587..2516)
                     /locus_tag="VFV20_08130"
     CDS             complement(1587..2516)
                     /locus_tag="VFV20_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014406828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5288356.1"
                     /translation="METVLRTRGLTKRFGSLVAVDHVDLDVREGEVFGYLGPNGAGKT
                     TTLRLLCALIAPTSGSAEIAGYRLGRDDAQIRTIVGILTEQPGLYERQSAWDNLLFYG
                     ALYGLTRATARAQAERFLRLMGLWDRKDEPVATFSRGMKQKMAIARAALHEPRILFLD
                     EPTTGLDPDAAKTVREFIVALRETGRTVFLCTHNLDEADRLCDRIAFFRHHVIRTDTA
                     DDLRAELYGRATEFRILPSPRPEDLARVRAVPGVHDARLENGSIVVETPDPLRTNPLL
                     VRALVEGGAEIAFVTERKAHLEDIYLRIVEGRE"
     gene            2575..>3877
                     /locus_tag="VFV20_08135"
     CDS             2575..>3877
                     /locus_tag="VFV20_08135"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5288357.1"
                     /translation="MTPPTETERKLRELSFLHEVAQLASSARDWDEMLRIVIDRTTDA
                     MGVEVSSLYLLERRDAVLRLVATNGLNRAYIGKATLRVGEGITGWVANARVPLAARDV
                     RADPRFKWVRGVDEERFTSMLSVPLLIRDEMVGVMNVQSVAPRTFDRTEIEFLQTIAN
                     QVAGIIEKGRLQREAERKLREVSALFEVSNVLTSTLDLDEVLALVVDRLVRVYPGSAG
                     AIFLREGEQLRERARSGEPGKATLDAAKQAVAEARPIVAADQFALPLLAGDRLLGAVA
                     LRVPDGTELGDEEVSFVGALANQAALAIDKASLSALERETADSLRELERARSEFVAVV
                     THDLRTPLSVIRGYLDLIAESKNGKRKIPIEEAVAQVGRLDQLVDRILTGVRADRPEI
                     ALRRTRFDLRGAVDATLTELAPVTRRHRLTRPRSGEPLWVRA"
BASE COUNT          573 a         1337 c         1401 g          566 t
ORIGIN      
        1 cggcgtctgc gcgaggtcgg catgcggatc gaggtcgacg acttcggcac cggatattcc
       61 tcgctcggcc ggctggtcga tctcccgatc gacgcgctca aggtcgaccg gcgattcgtg
      121 tcggcgatga cgcgtgacca ccgcagcgag gcgatcgtcc gcgcgtgtat cgcgctcgcg
      181 cacgatctcg gcctcgaggt ggtcgctgag ggcgtcgagg accgcgagac gtgggagctg
      241 ctgcgggacc tcggctgcga cacggcgcag gggtactaca tcggcgcggc catgccgctg
      301 gacgacctcg gccgatggct cggcgcgtgg cagcggcgcg tgccggcggc cgtcgcaaag
      361 cgcgagcgcg gcaccccgat cgccggccgg caggtgctcg tcgtggacga cgagcccgcg
      421 atcgtcgcga tgatccgcga cgtgctggag gacgccggct tcgaggtcgt caccgcggcg
      481 aacggtgtgg aagcgctgcg cgcggtcgag cgcggcgagc ccgcggtcgt actcctcgac
      541 atgcagatgc cgatcctcga cggcgccggc ttcgcccgcc tcctgcgtga gcgcggcatg
      601 cgcgtgccca tcgtcgtgat gacggccggc tcgagcgcgg agcgctgggc gcgcgagctc
      661 cccgccgatg cccacctcgc gaagccgttc gacatcgagg gattgctcgg cgtcgcgcgg
      721 cgctacgccg gcgcgaactg atccggcgcg acccggtcag cgccagcggc tcaggatgtt
      781 ctcgcgctgg aacgcgagcc gcgcgaggag gaacaccgcc gcgtcgatga cgatgaggaa
      841 cagcgagaac tgcacgaagg cctcgttgtt gatgacgacg cggccggtca cctgcgcgat
      901 gcccacgccg acgaccggca cgacgacgag gccgccgatc tgctgcgcga cgcggacgtc
      961 gttcacccgt gtcgagatga ggatgccgag gttcaccgag aggagcgtga gcagcggcac
     1021 catgacgatg atcgcgagga tccagcgcgg cgcggtcacc gcgttcaccg cggcctggct
     1081 gccgaggccc gacaccgacg cgaggtagat cgcgtacgcg atccacgtga cgatcaccga
     1141 cggcacggtc gaggcgaggc tcttggcgaa gagcagctcg ccgacgccga ccggagcggc
     1201 gagcagcggc tcgagggtgc gcgccgactt ctcgccgatg atcgagtaga tcgccatcgt
     1261 cagcggcacg acggtgggga tgagcatgaa gaggatgagg aagtaggtcg cgatgaaccc
     1321 ctggacggct tccttgggat ccaggccctg ggccgcgggc gcggcggcgt agagcgcctg
     1381 cacgtcgcgc tcgctcggcg ggttcgcggc ggcggcgccg acggcgacga taccggccat
     1441 gaggatcacc gcgggaggca ggagcgtcat gccgacgagc agcttgttgc cgagggcctc
     1501 gcgccattcg cggacgcaga ccgcgcgcag gagcgccagg cgatcgggat gacgcgcgat
     1561 gccggccgcg gggcgcgccg tcgcggtcat tcgcggccct cgacgatgcg caggtagatg
     1621 tcctcgaggt gcgctttgcg ctccgtgacg aacgcgatct cggcaccacc ttcgacgagc
     1681 gctcgcacca ggagcggatt cgtgcgcagc gggtcgggcg tctcgacgac gatcgagccg
     1741 ttctcgagac gcgcgtcgtg cactccgggc accgcgcgca cgcgcgcgag atcctcgggc
     1801 cggggcgagg gcaggatccg gaactccgtc gcgcgaccgt agagctccgc acgaaggtcg
     1861 tccgcggtgt ccgtgcggat gacgtggtgg cggaagaacg cgatgcggtc gcacaggcgg
     1921 tccgcctcgt cgaggttgtg cgtgcagagg aacaccgtcc ggccggtctc gcgcagcgcc
     1981 acgatgaact cgcggacggt cttggccgcg tccgggtcga ggccggtggt cggctcgtcc
     2041 aggaagagga tgcgcggctc gtgcagcgcc gcccgggcga tcgccatctt ctgcttcatg
     2101 cctcgcgaga aggtggcgac cggctcgtcc ttgcggtccc agaggcccat cagcctcagg
     2161 aagcgctccg cctgcgcccg cgccgtcgcg cgggtcaggc cgtacagggc gccgtagaag
     2221 agcaggttgt cccacgcgga ctgccgttcg tacagccccg gctgctcggt caggatgccg
     2281 acgatggtgc ggatctgggc gtcatctcgg ccgagccggt agccggcgat ctcggcgctg
     2341 cccgacgtcg gcgcgatgag cgcgcagagc agacgcagcg tcgtcgtctt ccccgcaccg
     2401 ttcggtccga gatacccgaa gacctcccct tcccgcacgt cgagatcgac atggtcgacc
     2461 gccacgagcg agccgaaacg cttcgtgagg cccctggtac ggagcactgt ctccatcgca
     2521 ccgagtctat gtgtcgcgct cgctcgcaca tcccctgttc ctatgattcc tttcatgacg
     2581 ccacccacgg agaccgagag gaagctgcgc gagctgtcct tcctccacga ggtggcgcag
     2641 ctcgcctcgt ccgcgcgcga ctgggacgag atgctgcgga tcgtcatcga ccgcacgacc
     2701 gacgcgatgg gcgtcgaggt gtcatcgctc tatctcctcg agcgccggga cgccgtcctc
     2761 cgtctcgtgg cgacgaacgg actgaaccgc gcctacatcg gcaaggcgac gctgcgcgtg
     2821 ggcgagggca tcaccggctg ggtcgcgaac gcccgcgtgc cgctcgcggc gcgtgacgtg
     2881 cgtgccgatc cgcggttcaa gtgggtccgg ggcgtcgacg aggagcggtt cacctccatg
     2941 ctctcggtcc cgctgctcat ccgcgacgag atggtcggcg tcatgaacgt ccagtcggtc
     3001 gcaccacgca ccttcgatcg aacggagatc gagttcctcc agaccatcgc caatcaggtc
     3061 gccgggatca tcgagaaagg ccggctgcag cgcgaggcgg agcgcaagct gcgcgaggtc
     3121 tcggcgctgt tcgaggtgtc caacgtcctc acctcgacgc tcgacctcga cgaggtgctc
     3181 gcgctcgtcg tcgacaggct ggtgcgcgtg tatccgggat cggcgggcgc gatcttcctt
     3241 cgcgaggggg agcagctccg cgagcgcgct cggtcgggcg agccggggaa ggcgaccctg
     3301 gatgccgcga agcaggccgt cgccgaggcg cgtccgatcg tcgccgcgga ccagttcgcc
     3361 ctcccgctgc tcgccggaga ccggctcctc ggcgccgtcg cgctgcgcgt gcccgacggc
     3421 accgagctcg gcgacgagga ggtctcgttc gtcggggcgc tggccaatca ggccgcgctg
     3481 gccatcgaca aggcgtcgct ctccgcgctc gagcgcgaga ccgcggacag cctgcgcgag
     3541 ctggagcgcg ctcgcagcga gttcgtcgcc gtcgtcacgc acgacctccg cacgccgctc
     3601 tcggtgatcc gcggctacct ggacctcatc gccgagtcga agaacggcaa gcgcaagatc
     3661 ccgatcgaag aggccgtcgc ccaggtgggt cgtctcgatc agctcgtcga tcggatcctc
     3721 accggcgttc gcgccgatcg cccggagatc gcgctgcggc gcacacgctt cgaccttcgc
     3781 ggcgccgtcg acgcgacgct caccgagctc gcgcctgtca cgcgacggca ccggctcacc
     3841 cggccgcgct ccggcgagcc gctgtgggtg cgcgcgg
//
LOCUS       DASTZR010000364         3106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_150808, whole genome shotgun sequence.
ACCESSION   DASTZR010000364 DASTZR010000000
VERSION     DASTZR010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150808"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VFV20_08140"
     CDS             <1..263
                     /locus_tag="VFV20_08140"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEU5288358.1"
                     /translation="DEFVILLPRTGLLPAQALVGRIAARLREETYHWAGAEHAVPRVS
                     FGIAWFPDDGRTADALLAVADERMYVDKSGASAARDDAADAD"
     gene            309..473
                     /locus_tag="VFV20_08145"
     CDS             309..473
                     /locus_tag="VFV20_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007691430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysW"
                     /protein_id="HEU5288359.1"
                     /translation="MPKCPECDGDVTVGSDAVKGEIVACPDCGAELEVTATSPVTLAL
                     APKAEEDWGE"
     gene            466..1335
                     /gene="lysX"
                     /locus_tag="VFV20_08150"
     CDS             466..1335
                     /gene="lysX"
                     /locus_tag="VFV20_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysX"
                     /protein_id="HEU5288360.1"
                     /translation="MSSPVVGVLLSRVRVEEKLLLQELASRGVETKVIDDRTLVLPTE
                     TKPDLGVDVVLERCIQHGTALYSLSVLDGWCIPTVNSYETAEVCGNKLLTTMRLTRAG
                     VPTPKTRIAFTPEAAMAAVEQMGYPCVMKPLIGSWGRLVSKVNDRESAEAIVEHKDVL
                     GNYLHSIYYVQEYIAKPGRDIRAFVVGDETICAIYRYSEHWITNTARGGRAENCPVTP
                     ELAELCVRSARAVGGGVLAIDVLERDGELLVNEVNYTMEFRNSIDTTGVNIPARMIDH
                     VLDVARGERRARA"
     gene            1332..2366
                     /gene="argC"
                     /locus_tag="VFV20_08155"
     CDS             1332..2366
                     /gene="argC"
                     /locus_tag="VFV20_08155"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEU5288361.1"
                     /translation="MKVGVIGAGGYAGGELLRLLLAHPSVTEIVAGSESLAGKPVTAA
                     HPNLRKRTDLQFVRYEQVTACDVLFLSLPNGSHRAGEFEDRAKLVIDLSSDHRLRDPA
                     DYQRWYGDAHPHPEALGRWVYGIPELHRAEIKGATRVTGAGCNATATLLALLPLFAAD
                     VVDRTRPVVVECKVGSSEGGREPSEDSHHPERVGVVRSFRPVGHRHTAEAEQELALGG
                     ARPRVDLSVTSVELVRGVLATAHVYVKQPLEEKDLWKVYRQRYASEPFVRIVKERTGI
                     FRYPEPKLLAGTNFADVGFAVDPRGDRVVALCAIDNLVKGAAGNGVQAMNVALGLDET
                     AGLGFVGLHP"
     gene            complement(2599..>3106)
                     /locus_tag="VFV20_08160"
     CDS             complement(2599..>3106)
                     /locus_tag="VFV20_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEU5288362.1"
                     /translation="LDAELAVIDSGIRHDHASGAYNARRAECEHAAELLGVATLRDVD
                     RDADLTGLPQTLAARVRHVVSENARVLAAVAAIEAGDARALGAVVSAGHASLRDDFQV
                     SLPEIDAIVGAAQRDADVHGARLTGGGFGGSVLVLARSGRARAAAERIVGATSGRIVV
                     PAGDAVES"
BASE COUNT          448 a         1092 c         1118 g          448 t
ORIGIN      
        1 gcgacgagtt cgtgatcctg ctgccccgca ccgggctcct gccggcgcag gcgctggtcg
       61 gccgcatcgc cgcgcggctg cgcgaggaga cgtatcactg ggcgggagcc gagcacgccg
      121 tcccgcgtgt ctcgttcggc atcgcctggt tcccggacga cggccgcacc gccgacgcac
      181 tgctcgcggt cgccgacgag cgcatgtacg tcgacaagtc cggcgcctcc gccgctcgcg
      241 acgacgctgc ggacgcggac tagcaccgtt acccttgtca cgatccagct gtggggaggg
      301 agtccgtcgt gccgaagtgt ccggaatgtg acggcgatgt caccgtcggg tccgatgcgg
      361 tgaagggcga gatcgtcgcg tgccccgact gcggtgcgga gctcgaggtc accgcgacgt
      421 cgccggtcac gctcgcgctc gcgccgaagg ccgaagaaga ctggggtgag tagccccgtc
      481 gtcggggtgc tgctctcgcg tgtccgcgtc gaagagaagc tgctcctcca ggagctcgcg
      541 tcgcgcggcg tggagaccaa ggtcatcgac gaccgcaccc tcgtgctccc gaccgagacg
      601 aagcccgacc tcggcgtcga cgtcgtgctc gagcggtgca tccagcacgg caccgcgctg
      661 tactcccttt cggtgctgga cggctggtgc atcccgacgg tgaactcgta cgagacggcc
      721 gaggtgtgcg gcaacaagct gctcaccacc atgcggctca cacgggccgg cgtgccgacg
      781 ccgaagacgc gcatcgcgtt cacgccggag gcagcgatgg cggccgtcga gcagatgggc
      841 tacccgtgcg tgatgaagcc gctcatcggc tcgtggggca ggctcgtctc gaaggtgaac
      901 gaccgcgaga gcgccgaggc gatcgtcgag cacaaggacg tgctcgggaa ctacctgcac
      961 tcgatctact acgtccagga gtacatcgcg aagcctggac gcgacatccg cgcgttcgtc
     1021 gtcggcgacg agaccatctg cgcgatctac cggtactccg agcactggat caccaacacg
     1081 gcgcgcggcg gcagggcgga gaactgcccg gtcacgcccg agctcgccga gctgtgcgtg
     1141 cggtcggcgc gcgccgtcgg cggaggcgtg ctcgcgatcg acgtgctgga gcgcgacggc
     1201 gagctcctcg tgaacgaagt gaattacacg atggagttcc gcaactcgat cgacacgacg
     1261 ggcgtgaaca tccccgcgcg gatgatcgac cacgtgctgg acgtggcgag aggcgagcga
     1321 cgagcgcgag catgaaggtc ggcgtgatcg gtgccggcgg ctacgccggc ggtgagctgc
     1381 tgcggctgct gctcgcgcat ccctcggtca cggagatcgt cgcggggagc gaatcgctgg
     1441 cggggaagcc cgtgaccgcg gcgcatccga acctgcgcaa gcgaaccgac ctccagttcg
     1501 tgcggtacga gcaggtgacc gcgtgcgacg tcctcttcct cagtcttccc aatgggtcgc
     1561 accgcgccgg tgagttcgag gatcgcgcga agctcgtcat cgacctctcc tccgatcatc
     1621 gcctgcgcga cccggccgat taccagcgct ggtacggcga cgcgcacccg catcccgagg
     1681 cgctcggacg atgggtgtac gggatccctg agctccaccg ggcggagatc aagggggcga
     1741 cgcgcgtcac gggcgccggc tgcaacgcca ccgcgacgct cctggcactc ctgccgctgt
     1801 tcgcggccga cgtcgtcgat cgcacgcgcc cggtcgtcgt cgagtgcaag gtcggatcga
     1861 gcgagggcgg tcgcgagcca tccgaggact cgcatcaccc cgagcgcgtg ggtgtggtgc
     1921 gttccttccg cccggtcggc caccggcaca cggcggaggc ggagcaggag ctcgcgctcg
     1981 gcggcgcgcg cccgcgcgtc gatctctcgg tgacgagcgt cgagctcgtg cgtggcgtgc
     2041 ttgccacggc gcacgtgtac gtgaaacagc cgctcgagga gaaggacctc tggaaggtct
     2101 accgccagcg gtacgcgtcg gagccgttcg tgcgcatcgt gaaggagcgg accggaatct
     2161 tccgctatcc ggagccgaag ctgctcgcgg gcacgaactt cgccgacgtg ggcttcgccg
     2221 tcgatccgcg gggcgaccgc gtcgtcgcgc tctgcgcgat cgacaacctc gtgaagggcg
     2281 cggccggcaa cggcgtccag gcgatgaacg tcgcgctcgg cctcgacgag accgccgggc
     2341 tcggattcgt cgggctgcac ccctagccgc gctctcccag acctgcggtc gcgtccgcgt
     2401 ggcggcgggt cctgtcccgc tccgggcgtc gttctcccct cgcgaagtcc ggctccccgg
     2461 cccgcccgtc ttccggtcgc gtgatggacc gcactgctac gggcgtccgg gtccggactt
     2521 cgcttcggtg agaacaacgc cctgcgcgtg ccacccgccg ccacgcggat gtgatggagg
     2581 cgcgtcagga gagcgcgcct agctctcgac cgcgtcgccc gcggggacga cgatgcgccc
     2641 ggatgtcgcg ccgacgatgc gctccgcggc ggcccgcgcc cttcccgacc gtgcgaggac
     2701 gagcacgctc ccgccgaagc cgccaccggt gaggcgcgcg ccatggacgt cggcgtcgcg
     2761 ctgcgcggcg ccgacgatcg cgtcgatctc cggcaggctg acctggaagt cgtcgcggag
     2821 cgaggcgtgg ccggcgctca cgaccgcgcc gagcgcgcgc gcgtcgcccg cctcgatcgc
     2881 cgcgaccgcg gccaagaccc gcgcgttctc ggacacgacg tggcgcacgc gagccgcgag
     2941 cgtctgcggc aggcccgtga gatccgcgtc gcggtcgacg tcgcgcagcg tcgcgacgcc
     3001 gagcagctcg gcggcgtgct cgcactcggc gcgccgcgcg ttgtacgcgc cggaggcgtg
     3061 gtcgtgccgg atgccggagt cgatcaccgc gagctcggca tcgagc
//
LOCUS       DASTZR010000365         2118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_198584, whole genome shotgun sequence.
ACCESSION   DASTZR010000365 DASTZR010000000
VERSION     DASTZR010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198584"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1605
                     /locus_tag="VFV20_08165"
     CDS             <1..1605
                     /locus_tag="VFV20_08165"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU5288363.1"
                     /translation="ILCTHEKVAVGVALGYARATGRPMGAIVHDVVGLLHSTLGVYYS
                     YLDKVPVIVMGATGPMDRRHRRPGIDWFHTAQVQGEAVRYFTKFDDQPASVADFPASF
                     ARAYRYATSEPAGPVYLCYDAGLQEEPLERPIAVDDVVAGARPSMINADPAALAKAAD
                     MLARAERPVILTEYTGRHPESVAHLVGLAEDLSAAVLDLHARMNFPNRHPLSLNGGDV
                     LKDADLVLAVDVGDLHRALNELDRDSRERAKRSRIAPGTPVIDLGVSELRQSKWTEDL
                     GDFQPTALSVVADTRLALPALRALLRERLGTRDRGPRRRELAAIHDAVRAGWEEKARQ
                     NWDAAPMSAARLASEVWNVIKGDDWVLTGADLEEWTMRLWDFDTPKRFPGHALGTATQ
                     ISTSLGVGLAYRGTDTLVVDIQPDGDLMFDPGALWTAANSRIPLLAVMYNNRAYYNDF
                     EHQIRVAGHRGTPVENAHIGQEIDNPAPDFAAMARSMGWYAEGPVVDPAQAGPAIARA
                     AAYVREKRMPALVDTIVRRRTPSRFR"
     gene            1621..>2118
                     /locus_tag="VFV20_08170"
     CDS             1621..>2118
                     /locus_tag="VFV20_08170"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5288364.1"
                     /translation="MLRRVLFDLRYRFGRPRWDTGITPPELVAFVESRAPGRAVDLGC
                     GTGTNVVYLASHGWDAFGIDFSPKAIGMAKRRAHANGITARTHFLIADVTRLPDLGAP
                     FDLALDMGCLHSVPPERRPDYARGLLARLAPGGTYLLYAFCPPDRYGIARDDVERLFS
                     TLRLAT"
BASE COUNT          295 a          805 c          724 g          294 t
ORIGIN      
        1 atcctctgca cgcacgagaa ggtcgcggtg ggcgtcgcgc tcggctacgc gcgcgcgacc
       61 ggccggccga tgggcgcgat cgtccacgac gtcgtcggtc tcctgcactc gacgctcggc
      121 gtgtactact cgtacctcga caaggtgccg gtcatcgtga tgggcgcgac cgggccgatg
      181 gaccgtcgcc accgtcgccc cgggatcgac tggttccaca ccgcgcaggt gcagggcgag
      241 gcggtccgtt acttcaccaa gttcgacgac cagcccgcga gcgtcgccga cttccccgcc
      301 tcgttcgcgc gcgcgtaccg ctacgccacg agcgagccgg cggggccggt gtacctgtgc
      361 tacgacgccg ggctgcagga ggagccgctc gagcggccga tcgcggtgga cgacgtggtc
      421 gccggagcgc gcccctccat gatcaacgcc gatcccgccg cgctcgcgaa ggcggcggac
      481 atgctcgcgc gcgccgagcg gccggtcatc ctgaccgagt acaccggccg gcacccggag
      541 tccgtcgcgc acctcgtcgg gctggccgag gatctctctg cagcggtcct cgacctgcac
      601 gcacgcatga acttcccgaa ccgccatccc ctctccctga acggcggcga cgtcctgaag
      661 gacgccgacc tcgtgctcgc ggtcgacgtc ggcgatctgc accgcgcgct gaacgagctc
      721 gaccgggaca gccgcgagcg cgccaagcgt tcgcgcatcg ctcccggcac gccggtgatc
      781 gacctcggcg tgtccgagct ccgtcagagc aagtggaccg aggatctcgg cgacttccag
      841 cccacggcgc tctcggtcgt cgccgacacc cgcctcgcgt tgccggcgct gcgcgcgctg
      901 ctgcgcgagc ggctcggcac acgcgaccgc ggcccgcggc ggcgcgagct cgcggcgatc
      961 cacgacgcgg tgcgcgcggg ctgggaggag aaggcgcggc agaactggga cgcagcgccg
     1021 atgagcgcgg cccgtctcgc gtccgaggtc tggaacgtca tcaagggcga cgactgggtc
     1081 ctcaccggcg cggacctcga ggagtggacg atgcggctct gggacttcga caccccgaag
     1141 cgcttccccg gacacgccct cgggacggcg acgcagatct cgacgtcgct cggcgtcgga
     1201 ctcgcctacc gcggcaccga cacgctcgtc gtggacatcc agccggacgg cgacctcatg
     1261 ttcgatcccg gcgcgctgtg gacggcggcc aacagccgca tcccgctgct cgccgtcatg
     1321 tacaacaacc gcgcttacta caacgacttc gagcaccaga tccgcgtcgc cggccaccgc
     1381 ggcacgccgg tcgagaacgc gcacatcggt caggagatcg acaaccccgc gccggacttc
     1441 gcggcgatgg cccgcagcat gggctggtac gcggaggggc ccgtcgtcga tcccgcgcag
     1501 gccggtcccg cgatcgcgcg cgccgcggcg tatgtgcgcg agaagcgcat gcccgcgctc
     1561 gtggacacga tcgtccgacg gaggaccccg agccgcttcc gctgagcgca gactcgttcg
     1621 gtgctgcgcc gcgtgctctt cgacctgcgc taccgcttcg gccgtccgcg gtgggacacc
     1681 ggcatcacgc cgccggagct cgtcgcgttc gtggagtcgc gtgcgccggg acgcgcggtc
     1741 gacctcggct gcgggacggg cacgaacgtc gtctacctcg cgagccatgg ctgggacgcg
     1801 ttcggcatcg acttctcgcc gaaggcgatc ggcatggcga agcggcgcgc acacgcgaac
     1861 ggcatcacgg ctcggaccca cttcctgatc gccgacgtca cccgcctgcc cgacctcggc
     1921 gcgccgttcg acctcgccct ggacatgggc tgcctgcatt ccgtcccgcc ggagcgccgg
     1981 cccgactacg cgcgcgggct gctcgcgcgc ctcgcgcccg gcggcacgta cctgctgtac
     2041 gcgttctgcc cgcccgaccg ctacggcatc gcgcgtgacg acgtcgagcg gctcttcagc
     2101 acgttgcggc tcgccacg
//
LOCUS       DASTZR010000366         2674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_41485, whole genome shotgun sequence.
ACCESSION   DASTZR010000366 DASTZR010000000
VERSION     DASTZR010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41485"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="VFV20_08175"
     CDS             <1..982
                     /locus_tag="VFV20_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288365.1"
                     /translation="TIATATGGAPDRVFLYEIAARATLARPILGYGPDNFRVAHAEHR
                     TVASLAFGAGPESTAHDRFLDASATTGILGLLLLCALVVVGTIELRRLATARSSVGAP
                     LFIGWLAYWAHALVAAESMAASWYPWVALGLAAGLRGIRSADRPPHGVPRLAVAALAV
                     IAVIGAASGARAMQANRDAWTSAEAAHFGDLATATEFADRAAARDGGRADHWNRLGLA
                     YEAQQRWQPAVAAYREAAGRGPYVAVYWANLARGLARVAGSDPSARDEAVAAARRATV
                     VDPNAPVGHVVLAEVATAFGRCELALAEASVAASLERGHDQLIERARGCR"
     gene            complement(1044..1544)
                     /locus_tag="VFV20_08180"
     CDS             complement(1044..1544)
                     /locus_tag="VFV20_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288366.1"
                     /translation="MKKPNVILRALAVAAGVLILGGGALAAENLDLVGSVDNDTSGQT
                     AAEHATKHADLPSVAAGVIAALTGGSNPSTTVSQRDAEHGNATASEHASANASKHDED
                     ADNDTDEDNDTDVDNEVSEGQEVGDTGTAGTKPGWGCGDRNHEHSGPPGRPGATPPPG
                     CTKSHD"
     gene            complement(1731..2642)
                     /locus_tag="VFV20_08185"
     CDS             complement(1731..2642)
                     /locus_tag="VFV20_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5288367.1"
                     /translation="MTTNPAAAGEGLAHWATKGDVRLFLWERGPSGGAARGTILFVHG
                     SSMASQPTFDLRIPGRPSAMEHFAGLGYDTWCVDMEGYGRSDKHRPINSDIATGADDL
                     LAAAEYIRRVRGAERLLLYGISSGALRAALFAQRHPDRVSRLALDAFVWTGEGSPTLA
                     QRRTRLAEYKSRLRRPIDRAFVRSIFTRDHPGTADDVVVDAFADAILALDDSMPTGTY
                     VDMCENLPIVDPERITAPTIVMRGEHDGIAALPDLLEFFRRLPNPDKQFVIMPGIAHA
                     SFQQKNYELVYHVLASFFAQPVPVYGG"
BASE COUNT          359 a          924 c          959 g          432 t
ORIGIN      
        1 caccatcgcg accgcgacgg gcggcgcgcc agaccgcgtc ttcctgtacg agatcgccgc
       61 gcgcgcgacg ctcgcgagac cgatcctcgg ctacgggccc gacaacttcc gtgtcgcgca
      121 tgcggaacac cgtacggtgg cgagcctggc gttcggtgcg ggtcccgagt ccaccgccca
      181 cgaccggttc cttgatgcgt cggccacgac gggcatcctc ggactgctcc tcctctgcgc
      241 gctcgtcgtc gtcgggacga tcgagctccg gcgcctcgcg accgcgcggt cgagcgtcgg
      301 tgcgcccctc ttcatcggat ggctcgcgta ttgggcccat gcgctcgtcg cggcggagtc
      361 gatggcggcc tcgtggtacc cgtgggtcgc gctcgggctt gcagcgggcc tgcgcggcat
      421 ccgtagcgca gatcgtccgc cgcatggcgt gccgcgcctc gcggtcgcgg cgctcgcggt
      481 gatcgccgtc atcggcgcgg cgagcggcgc gcgcgccatg caggcgaacc gggacgcatg
      541 gacgtcggca gaggcggccc acttcggcga cctcgcgacg gccacggagt tcgcggaccg
      601 cgccgcagcg cgcgacggcg ggcgcgcgga ccactggaat cgccttggcc tcgcgtacga
      661 agcgcagcag cgctggcagc cggcggtcgc ggcctaccgt gaggcggcgg gacgcggtcc
      721 gtacgtggcc gtgtattggg ccaacctcgc gcgcggtctg gcacgggtcg cggggagcga
      781 cccgtcggct cgcgatgagg ccgtcgcggc ggctcggcga gcgacggtgg tggacccgaa
      841 cgcccccgtg ggtcacgtcg tcctggcgga ggtcgcgacc gcgttcgggc gttgcgagct
      901 cgcgctcgcc gaggcgagcg tcgccgcgtc gctggaacga ggccacgatc aactcatcga
      961 gcgggcccgc gggtgccgct gaaatgaaga agcggagcgg gggatcgccc cgctccgcct
     1021 ctgtcagtca gaccttcgcg cgatcagtcg tgcgacttgg tgcaacccgg aggcggggtc
     1081 gcgcccggcc ggccgggagg gccgctgtgc tcgtggttgc ggtcgccaca gccccagccg
     1141 ggcttggtgc cggcggtgcc ggtgtcgccg acctcttggc cctcggagac ctcgttgtcg
     1201 acgtccgtgt cgttgtcctc gtccgtgtcg ttgtccgcgt cctcatcgtg cttggacgcg
     1261 ttcgcgctcg cgtgctcgct tgcggtcgcg ttgccgtgct cggcgtcgcg ctgcgaaacg
     1321 gtcgtggacg ggttcgatcc gccggtgagc gcggcgatca cgccggccgc gacggatgga
     1381 aggtctgcgt gcttcgtcgc atgctcggcc gccgtctgcc cgctcgtgtc gttatcgacc
     1441 gagccgacga gatcgaggtt ctcggccgcg agggcgcctc ccccaaggat caacacgccc
     1501 gctgcgaccg cgagtgcgcg aaggatcacg ttcggtttct tcattgggcc tcctcctgtc
     1561 ggtgcgatgt cacgttcgtc cagcggactg caggtatgcc tccgctgcac gcttcaacgg
     1621 gccccctacg catgccgtta ggagatcgca gccaagacgt cgtattgggc gcatgccgta
     1681 acgaccgctc gccactcagt ccgaatgacg agcaagcgca ggagtgccgg ctagccgccg
     1741 tacaccggaa cgggctgcgc aaagaaggat gcgaggacgt gatacacgag ctcgtagttc
     1801 ttctgctgga agctcgcgtg cgcgatcccc ggcatgatga cgaactgttt gtcggggttc
     1861 ggcagacggc ggaagaactc gagcagatcc ggcagtgcgg cgatcccgtc gtgctcaccg
     1921 cgcatcacga tcgtcggcgc cgtgatgcgt tcgggatcca cgatgggcag gttctcgcac
     1981 atgtccacgt aggtgccggt cggcatcgag tcgtcgagcg cgaggatcgc gtcggcgaag
     2041 gcgtcgacga cgacgtcatc cgccgttccc gggtgatcgc gggtgaagat gctgcgcacg
     2101 aacgcgcgat cgatcggacg gcgcagcctg ctcttgtact ccgcgaggcg cgtgcgccgc
     2161 tgggccagcg tcgggctgcc ctcgccggtc cacacgaacg cgtcgagcgc gagacgcgag
     2221 acccggtcgg gatgccgctg cgcgaacagc gccgcgcgga gcgcccccga ggagatgccg
     2281 tagagcagga gccgctctgc gccccggacg cgccggatgt actccgcggc ggccagcaga
     2341 tcgtcggcgc cggtcgcgat gtcgctgttg atcggccggt gcttgtcgga ccgtccgtag
     2401 ccctccatgt ccacacacca ggtgtcgtac ccaagcccgg cgaagtgctc catcgccgat
     2461 ggccggcctg ggatccggag gtcgaacgtc ggctgagagg ccatcgacga cccgtggacg
     2521 aacaggatgg tccctcgcgc ggcgccaccg gacgggccgc gctcccacag gaagagccgt
     2581 acgtcgccct ttgtggccca gtgcgccaat ccttcgcccg cggccgccgg attcgttgtc
     2641 aaagcctgat cctcccctga tgatgcgacg tacc
//
LOCUS       DASTZR010000367         3871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_176931, whole genome shotgun sequence.
ACCESSION   DASTZR010000367 DASTZR010000000
VERSION     DASTZR010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176931"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1528)
                     /locus_tag="VFV20_08190"
     CDS             complement(<1..1528)
                     /locus_tag="VFV20_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288368.1"
                     /translation="MKSAPVGVVFVHGIGSQGQSSTLREFAAPLLDYLREWHRTRGVP
                     EWQPDAAELSYGGTLHGPARVMLGIPAHAQGETVWPERRFIFAEAWWAARLDPPSLDR
                     MIAWSARSLWRVIRTLFTQMTRRMAFLLGRGGTSESDPGPLAALGEVLSAFGFFAAYV
                     AGAVLGYVVLLPLLVLAHIPIREVQEFVLVKMLKPFLVENIGDFATYIDDEVQALNIR
                     SAVGDAVRALVGLGCDEVCVVAHSQGALVAFDGVCALGREDPAAAAHVRKLVTFGGAL
                     NRAFAEPGCPPRLKGTLPSHVFWLDVWSQYDPVAGGYLRRRNTASPLVAPSTALRQRM
                     GWSDRFDGPLPDQVTNGMNPLLDHGGYFQNPEQFVNRVLAEVEDPSAYYKTSRFFFRG
                     DSPFRSDARRVYRRRTRISALAWWRLWAIGTLGFTLLWRGAARLGPDGAAVWAQLATV
                     PGHELLAAPGKLFDGIGMVLDAVAARLVALGVLPDLIDAIRAWWSRTAWSDVILASVV
                     L"
     gene            1832..2416
                     /locus_tag="VFV20_08195"
     CDS             1832..2416
                     /locus_tag="VFV20_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin-like protein"
                     /protein_id="HEU5288369.1"
                     /translation="MAIGVFGARVTTASAQTVFCNAATSHCYEVVAVPAGITWPNAKA
                     AAEARTYSGFSGHLVTITSQAETNFLVARGLTPAAYWIGGSQPLGQTGAATNWSWVTG
                     EPFVYTNWETGEPNDYYGPASEQYLEFWLTPGKWNDQNSTFLQGGYVVEYDQPFDLDD
                     CKDGGWMAYQVFKNQGDCVSWIATAGKNEPANMP"
     gene            complement(2525..2929)
                     /locus_tag="VFV20_08200"
     CDS             complement(2525..2929)
                     /locus_tag="VFV20_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEU5288370.1"
                     /translation="MTRAGPVDELIAKTPDWRGAMLAKLRKIVHDADPEVAEDVKWKR
                     PSNPLGSAVWSHDGMVCVGIILKERVRLSFSAGSSLPDPKKLFNAQLLGKSRAIDVAQ
                     NEKLDEPSLKAIVKAAVAHNLAKTRPAKTRAR"
     gene            3474..>3871
                     /locus_tag="VFV20_08205"
     CDS             3474..>3871
                     /locus_tag="VFV20_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288371.1"
                     /translation="MTSDPTLPGGVALLVFIAVVSIAFGNIVRRRSGATIGVAGGTTL
                     FLGLLSASMAIGHLVGVVGMRGDYYAYDFRSAALFLVGMTIFAAGALCIAAARGLQRG
                     QRDWSGRALDGTLLLLLVCGPLIPVQGLAVV"
BASE COUNT          641 a         1290 c         1325 g          615 t
ORIGIN      
        1 cgagcacgac gctcgcgagg atgacatcgc tccacgcggt ccgggaccac catgcgcgga
       61 tcgcgtcgat caggtccggc aggacgccga gcgcgacgag tctcgcggcg acggcgtcga
      121 gcaccatgcc gatcccgtcg aagagcttcc cgggtgcggc cagcagctcg tgaccgggaa
      181 ccgtcgcgag ctgcgcccat acagcggcgc cgtccgggcc gaggcgcgcg gcaccgcgcc
      241 acagaagcgt gaagccgagc gtgccgatcg cccagaggcg ccaccacgcg agcgcgctga
      301 tgcgtgtgcg ccggcgatac acccggcgcg catccgatcg gaacggcgaa tcgccgcgaa
      361 agaagaaccg cgacgtcttg tagtaggccg acgggtcctc gacctccgcg agcacgcggt
      421 tcacgaactg ctcggggttc tggaagtagc cgccgtggtc gagcaacggg ttcatgccgt
      481 tcgtgacctg atccggcagc gggccgtcga agcgatcgct ccatcccatg cgctgccgca
      541 gtgccgtact cggcgccacc agcggcgacg ccgtgttgcg tcggcggagg tagccgccgg
      601 caacggggtc gtactgggac cagacgtcga gccagaagac gtgcgacggc agcgtcccct
      661 tgagccgggg cgggcatccc ggctccgcga acgcgcgatt gagcgcgccg ccgaacgtga
      721 cgagcttgcg cacgtgcgcg gccgcggcgg gatcctcgcg gcccagcgcg cacacgccgt
      781 cgaacgcgac gagcgcgccc tgcgaatgcg ccacgacgca gacctcgtcg cagccgagcc
      841 cgacgagcgc gcggacggcg tcgcccaccg ccgaccggat gttcagcgcc tgcacctcgt
      901 cgtcgatgta cgtggcgaag tcgccgatgt tctcgaccag gaacggcttc agcatcttca
      961 cgagcacgaa ttcctgcact tcgcggatgg ggatgtgcgc gagcacgagc agcgggagca
     1021 ggacgacgta gccgagcacg gcgcccgcga cgtacgcggc gaagaagccg aaggcgctca
     1081 ggacctcgcc gagcgccgcg agcggaccgg gatcgctctc gctcgtgccg ccgcgtccta
     1141 ggaggaacgc catccgccgg gtcatctgcg tgaagagcgt ccggatcacc cgccacagcg
     1201 agcgcgcgct ccacgcgatc atccgatcga gcgacggcgg atcgaggcgc gcggcccacc
     1261 aggcctccgc gaagatgaag cggcgctcgg gccacacggt ctcgccctgc gcgtgcgccg
     1321 ggataccgag catgacgcgc gcgggtccat gcagggtgcc gccgtacgag agctccgcgg
     1381 cgtcgggctg ccactcgggc acgccgcgtg tgcggtgcca ctcgcgcagg tagtcgagaa
     1441 ggggcgcggc gaactcgcgc agcgtcgagc tctggccctg gctgccgatg ccgtgcacga
     1501 acacgacgcc gacgggcgcg gacttcattg agcgcggact atgacgcaga cgtcgacgcg
     1561 cagggtgagg gccgggtatc gcgccggctc ggtcccctgc cgttcccaca caccgacacc
     1621 cgagcgtgcg gggccagtgg cgtggtgcgg gcgcgcgcag cgtgctcacg atggctcgcg
     1681 cttggacaga gacgatgcag accccgttcg cggccactcc taccgtcaca atccgtcccc
     1741 agccaaatca gaaccgggac gacggaggag agaagacatg cgaacgcgta tgcgactcgt
     1801 gcggtgcgtg gggttgagtg ccgtgctggc gatggctatc ggggtcttcg gcgctcgcgt
     1861 caccaccgcg agcgcgcaga ccgtcttctg caacgcggca acaagccact gctacgaggt
     1921 cgtggcggta cccgcgggga tcacctggcc gaacgcgaag gcagcggccg aggccaggac
     1981 ctacagcggc ttttcgggtc acctcgtcac gatcacctcg caggcggaga ccaacttcct
     2041 ggtggccaga ggtttgaccc cggccgcgta ttggatcggc gggtcgcagc cgctcggaca
     2101 gacaggggcg gcgaccaact ggtcctgggt gaccggcgag ccgttcgtct acacgaactg
     2161 ggagaccggc gagccgaacg actactacgg tccagcgagc gagcagtact tggagttctg
     2221 gctcactccg ggcaagtgga acgaccagaa cagcaccttc ctgcaggggg gatacgtcgt
     2281 cgagtatgac cagccattcg atctcgacga ctgcaaggat ggcggctgga tggcgtatca
     2341 ggtcttcaag aatcagggcg actgcgtgag ctggatcgcg accgccggaa agaacgagcc
     2401 ggcgaacatg ccatagcccg gcgcaaattc cagtgtcagg ggagcgaagg gagccgccca
     2461 catcgggcgg ctctctttct gaccgcgact gcgatctggc gccaggcccg gtattacttg
     2521 gtgctcatcg cgctcgcgtc ttcgccggcc gcgtcttcgc gagattgtgc gcgacggcgg
     2581 ccttcacgat cgccttgagc gacggttcat cgagcttctc gttctgagca acatcgatcg
     2641 cccgcgactt gcccaagagc tgcgcgttga agagcttctt ggggtccgga aggctcgagc
     2701 ccgcggagaa cgagagcctc acccgctcct tgaggatgat cccgacgcac accatcccgt
     2761 cgtgcgacca gacggccgag ccgagtgggt tgctgggccg cttccacttc acgtcctccg
     2821 cgacctccgg gtccgcgtcg tggacgatct tgcggagctt cgccagcatc gcgccgcgcc
     2881 aatcgggcgt cttcgcgatc agctcatcga ccggtcccgc tcgtgtcatg tcagcgccac
     2941 gctccttgcg tctatcgggc cgaaccgccg gccacggggg gacgttagcc catcgcggcg
     3001 tgcggcaatt gacacgcgag ccgcgtcgta cgaattgaat tcgctaggaa gccaacttgc
     3061 cgacggccag gatcgaactg gcgacacatg gttcttcagc cgtttgcgcc ggaagccgtg
     3121 gagctccgtc cagagcaccg ccgcgttgcg cagggcattg ggaccaacgg cgaccgcatg
     3181 gaccatggtg acgagctcgc gttcgctgta gccgggcatg cccttcgcgg acgcttgggt
     3241 gtctttacct cgcgtaggac cgacaggcga acgtcgtcgg tgccggcgta ccagatgcgc
     3301 ttgcgggcga gataggtcgc caggctcttg agcgcgatga tcttgttgcg ccgcgtgttc
     3361 acgtcttcgc gttcgctggg aaacgaagct ccgcctcttg gacaagtcgg gctgagacca
     3421 tttctggcgc agccactgag acgacggttc ggaacgcgca ccatacggaa gccatgacga
     3481 gcgatccaac gcttcctggg ggtgtcgcgc tccttgtctt cattgccgtc gtcagcatcg
     3541 cctttggcaa catcgtgcgt cgtcgctctg gcgccacgat cggcgtcgct ggtgggacga
     3601 cgctcttcct cgggctgtta agcgcatcta tggcgatcgg acacctggtt ggcgtggtcg
     3661 ggatgcgtgg tgactactac gcgtacgact tccggtcagc ggcgctcttc cttgtcggga
     3721 tgacgatctt cgctgccggc gcgctgtgta tcgcggcagc gcgcgggcta cagcgtggac
     3781 aacgtgactg gtcgggtcgg gcccttgacg gcacgctctt gctcctcctc gtatgtggtc
     3841 cgctcatccc cgttcagggc ctcgcggtcg t
//
LOCUS       DASTZR010000368         2919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_41605, whole genome shotgun sequence.
ACCESSION   DASTZR010000368 DASTZR010000000
VERSION     DASTZR010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41605"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VFV20_08210"
     CDS             complement(<1..525)
                     /locus_tag="VFV20_08210"
                     /EC_number="2.1.3.15"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEU5288372.1"
                     /translation="MPFGRKPKEFPSHLWTQCPNCGEMLFNKQLVRNLSVCTKCEHHF
                     RMGARARVELLVDKGSFVEQDQALASADPLSFTDSKPYPERIAAAQAKSGEKDAAIAG
                     TATIGGNPIELVVMDFDFMGGSMGSVVGEKITRAAERALAARTALVVVSASGGARMQE
                     GTLALMQMGKIVSAI"
     gene            complement(534..1397)
                     /locus_tag="VFV20_08215"
     CDS             complement(534..1397)
                     /locus_tag="VFV20_08215"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU5288373.1"
                     /translation="MSDRFAIVVDGTACLTDDVEREFDIRWLPLHVDIGQESFTANVD
                     LSAEQFYAKIAAPGVLPGTSQPSMGECRDVYDAIVNSGTTKLIVLTVATELSGTFSVA
                     TTTAQAMPGVQIEVIDSRSLAGGISLIATAVGRLRRSGGSFEEAVALARKMAGRIQIL
                     AVADTLEYLKRSGRVSGAAALFGSMLQVKPILEVKEGVVHPLDKVRTRDKAVARLKEV
                     VSQRVPAGSRIHACTLHTNDPERARALGEWVQEQFHCVEHWIAEAGPVIGARAGPGVV
                     GLCWYREDEAK"
     gene            complement(1516..2286)
                     /locus_tag="VFV20_08220"
     CDS             complement(1516..2286)
                     /locus_tag="VFV20_08220"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEU5288374.1"
                     /translation="MARLALLAHPAVASAFGRELGRDVEYHARLASTQDRARALAEKG
                     AVRGVVVADEQTAGQGTHGRAWVSPPATSLLASWILRPAPAAPALFAALAGVAIARAL
                     EALGVGGARLKWPNDVELSGRKVAGALAHATSDGGGGVLVLGIGVNAHQRAADFPPEL
                     RGLATSCALAGTRVDRLALLARLTRELDRLEVPADRGSAMDEWRARSTLLGKPVTVRI
                     EGRDAFTATATAIDDEGALVVHTPSGVERIVTGDVRLA"
     gene            complement(2286..2651)
                     /locus_tag="VFV20_08225"
     CDS             complement(2286..2651)
                     /locus_tag="VFV20_08225"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HEU5288375.1"
                     /translation="MRATASPAASASAGALTSAKGAITVRAPLAGARVTSPLTIAGDA
                     SVFEANLQWRIVDAGGRALVEGIATASAGAPERGTFSVTATFTPPSADTSAVVEVYTR
                     SPKDGQIDDVVRVPVVIAR"
BASE COUNT          405 a         1091 c         1019 g          404 t
ORIGIN      
        1 gatggcggac acgatcttcc ccatctgcat gagcgcgagc gtgccctcct gcatgcgtgc
       61 gccgccggac gcggacacga cgacgagcgc cgtccgtgcc gcgagcgcgc gctcggcggc
      121 acgcgtgatc ttctccccga ccacgctgcc catcgagcca cccatgaagt cgaaatccat
      181 gacgacgagc tcgatgggat tgccgccgat cgtcgcggtc cccgcgatgg ccgcatcctt
      241 ctcgccgctc ttcgcctgcg cggccgcgat ccgctcgggg tacggcttgc tgtcggtgaa
      301 cgacagcgga tcggctgagg ccagcgcctg atcctgctcg acgaacgagc ccttgtcgac
      361 gagcagctcc accctcgcac gcgcgcccat ccggaaatgg tgctcgcact tggtgcacac
      421 gctgaggttg cggacgagct gcttgttgaa gagcatctcc ccgcaattcg gacactgcgt
      481 ccacaggtgc gacgggaact ccttcggctt tctgccgaag ggcatccgga gtctcacttc
      541 gcctcgtcct cacggtacca gcagagcccg acgacgccgg gacccgcgcg agcgccgatg
      601 acgggacccg cctcggcgat ccagtgctcg acgcagtgga actgctcctg cacccattcg
      661 ccgagcgcgc gcgcgcgttc gggatcgttc gtatgcagcg tgcacgcgtg gatgcgcgac
      721 ccggcgggta cgcgctgcga cacgacctcc ttcaggcgcg cgaccgcctt gtcgcgcgtg
      781 cggaccttgt ccagcgggtg caccacgcct tccttgacct cgaggatcgg cttcacctgg
      841 agcatggagc cgaagagcgc cgccgcgccg ctcacgcgcc cggagcgctt cagatactcg
      901 agcgtgtcgg cgaccgcgag gatctggatc cgcccggcca tcttccgcgc gagcgcgacg
      961 gcctcctcga acgagccgcc gctgcggcgc aggcggccca cggcggtcgc gatgaggctg
     1021 atgccgccgg ccagcgaacg tgaatcgatc acctcgatct ggacaccggg catcgcctgc
     1081 gccgtcgtcg tcgcgaccga gaacgtaccc gacagctcgg tcgcgacggt gagcacgatc
     1141 agcttggtcg tgccggaatt gacgatggcg tcgtacacgt cacggcactc gcccatcgac
     1201 ggctgcgacg tgcccggcag cacacctggg gccgcgatct tcgcgtagaa ctgctccgcg
     1261 ctcagatcga cgttcgccgt gaaggactcc tgtccgatgt cgacgtgcag gggcagccag
     1321 cggatgtcga actcgcgctc gacgtcgtcc gtgagacacg ccgtcccgtc gacgacgatc
     1381 gcgaagcggt cgctcatgct cttcgcgctc ggctcgcgcg gcctgcgccg cgctgcgcct
     1441 cgccggctcg acaccgctcg tcgagctcgc tcctcgcgag tgaatcgctc gtcgctcgcg
     1501 ctccttcgat ccgcgtcatg cgagccgcac gtcgccggtg acgatccgtt cgacgccgga
     1561 tggcgtgtgc acgacgagcg cgccctcgtc gtcgatggcg gtcgccgtgg cggtgaaggc
     1621 gtcgcggccc tcgatcctga cggtcaccgg cttgccgagg agcgtcgagc gcgcacgcca
     1681 ttcgtccatc gcggagccgc ggtcggcggg aacctcgagg cgatcgagct cacgcgtgag
     1741 gcgcgcgagc agtgcgaggc gatcgacccg cgtgccggcc agcgcgcagg acgtcgcgag
     1801 gccgcgcagc tccggcggaa agtccgcggc ccgctgatgc gcgttcacac cgatgccgag
     1861 cacgagcacc cctccgcctc cgtctgacgt cgcgtgcgcg agcgcgccgg cgaccttccg
     1921 ccccgagagc tcgacgtcgt tgggccattt taggcgggcg ccccccaccc cgagcgcctc
     1981 gagggcgcgc gcgatcgcga ccccggccag ggcggcgaac agcgccggcg cggcgggcgc
     2041 gggacgcagg atccatgacg cgagcaggct cgtggccggc ggggacaccc aggcgcgtcc
     2101 gtgcgtcccc tgcccggcgg tctgctcgtc ggcgacgacg acgccgcgga ccgctccctt
     2161 ctccgcgagc gcccgcgcgc ggtcctgcgt ggacgcaagg cgcgcgtggt actccacgtc
     2221 gcggccgagc tcgcggccga aggcggacgc caccgcggga tgcgcgagga gcgcgagccg
     2281 cgccactagc gcgcgatcac gaccgggacg cgcacgacgt cgtcgatctg gccatccttc
     2341 gggctgcgcg tgtagacctc gaccacggcg ctcgtgtcgg ccgagggcgg cgtgaacgtg
     2401 gcggtcacgc tgaacgtacc tcgttcgggc gcgccggcgc tcgcggtcgc gatgccctcc
     2461 acgagcgcgc ggccgccggc atcgacgatc cgccactgca ggttcgcctc gaacacgctg
     2521 gcatcgcccg cgatggtgag tggcgaggtc acgcgcgcgc ccgccagcgg ggcgcggacg
     2581 gtgatggctc ccttcgccga cgtgagcgcc cctgctgacg cgctcgcggc cggcgacgcg
     2641 gtggcacgca ccgacgcggt tggcgtggac ggaatacccg cggcgccgcc gccgcatgcg
     2701 gcgacgagca cgaggagggc ggccgacagc gaccggagcg cgctcagcgg tcgcgaccgt
     2761 cgcgcaggag ctgggctccc gcggcgcgca cgcgcagcat ccgcgcacgc cccgacacgt
     2821 cgtgggccgc ggcggtctcc tccatcgccg cgacgacccg cggcgcgaga cgcacgggcg
     2881 cgatcgccag gacgctcggg cccgcacccg acagcgccg
//
LOCUS       DASTZR010000369        17981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_672129, whole genome shotgun sequence.
ACCESSION   DASTZR010000369 DASTZR010000000
VERSION     DASTZR010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 17981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17981
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672129"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2275)
                     /gene="ileS"
                     /locus_tag="VFV20_08230"
     CDS             complement(<1..2275)
                     /gene="ileS"
                     /locus_tag="VFV20_08230"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEU5288376.1"
                     /translation="MSRTFAAVGSSFDLPELEHGVLDLWEREKSFETLRKKNAGGPRY
                     SFIDGPITANIEAMGVHHAWGRTYKDIYQRYKAMQGFDQRYQNGFDCQGLWVEVQVER
                     ELNLNSKRDIETYGIDKFSRACRARVDRSAAAITGQSRRLGQWMDWDNSYFTYSDNNI
                     SHIWHVLKLCHEKGWLIRGHRAMPWCARCGTALSEHEMTGSYKEMTHTSLYVRFPVLP
                     DGRGADLATQRASFLAWTTTPWTLPANVALAVHPDLDYAKVRVVADGATKPELLILGA
                     HVYAQAKWHNAELLGTVKGRDLLGLRYTGPFDELPVGATSGPAHTVIAWDEVGEAEGT
                     GIVHIAPGCGAEDFALAKPNGLPVIVPIDENANFVSGFGWLEGRQARDVAHEIADDLR
                     KRGRLFREVPYTHSYPVCWRCKEEIVFRVADEWFISMDELRPKLKDAAAKVRWLPPHT
                     GDRMQNWLDNMGDWNISRKRYWGLPLPFYNCTNGHFFILGSEQELRERAVRGLEQLQE
                     LHRPWIDNVIVACPTCKAESTRVRETGDVWLDAGIVPFSTLNWLTDKEQWKRWFPAEF
                     ITENVEQIRLWYYSQLFFSTVLAGRAPYDTVLSNEFVYDENGEEFHKTGDNFIDFPQA
                     AERAGSDVVRWFYSRHDPAEKVLFGYHVLGDVKRRLLTLWNTYAFFVTYANLDRFDPA
                     APAVAIAERPLIDRWLLSTLHRLIRDVRAALDQYDSQTACLRMEAFWDDLSTWYVRRN
                     RARFWKSKSERDSLAAYQ"
     gene            complement(2582..3247)
                     /locus_tag="VFV20_08235"
     CDS             complement(2582..3247)
                     /locus_tag="VFV20_08235"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEU5288377.1"
                     /translation="MYVKGWWDALAAPWRACLEEAWDAYRAGSNPIGACVADAAGRVV
                     GRGRNRIRESSPPAPQLAGHRLAHAEVNALLDASTWDGEPRSLTVYTTVEPCPLCAGA
                     IAMADVRAVRYASRDPWAGSASLFVATPYLRAKGISFAGPRDDLEVALKGMGIEYLLT
                     VGGRRITEIVGAAREAMPAGVVLGESLRRSGELRALASSGARAETAIGRLVALASEVV
                     PVH"
     gene            3295..3960
                     /locus_tag="VFV20_08240"
     CDS             3295..3960
                     /locus_tag="VFV20_08240"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEU5288378.1"
                     /translation="MAKTARPQPKERGEVACLARPAFETALAASLEREPVATLLDVDL
                     DGLLDLNERGGHEAGDRAIAAVTQALAKVAKAEGWILGRIGGDEFALLAPGVALEPAF
                     LRADQIRRDADAALAKALPGAHRCTVTIGVANTPRDTKQRGKDAANELLRRADLALHA
                     AKEQGGDTVGLTPADDMVLKSSYYSGAQLGRLKGLAERMKKKEAVLLREALDDLLRKY
                     QRS"
     gene            3970..4563
                     /locus_tag="VFV20_08245"
     CDS             3970..4563
                     /locus_tag="VFV20_08245"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU5288379.1"
                     /translation="MDLPPTVVRELDDLARRFGEPLRLAAPIDDSFTDPIWKRDRFGE
                     VCMVVRRPSGKLLLSIKTFYPRGAHRLPTGGIHHGEGVFDALVRETHEETGLVTAPRR
                     FLARIAYDPLSTPGAPPVFHTFAFLLDEVAGTLGAIDTSERIEEWKEIDVADLPRVAH
                     TLDHLTAQGTEDIGGDWAAWGRFRAVVHRVVHEALSV"
     gene            complement(4560..5648)
                     /locus_tag="VFV20_08250"
     CDS             complement(4560..5648)
                     /locus_tag="VFV20_08250"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5288380.1"
                     /translation="MGQILRYTLMLLRSYTRDRPALFFGFFFPLIFMMLFGILDLGAV
                     GHVNLGIADEARNADSERFIATLRGIETFKITSGTRADESGKLEAGDRDLVLVLPADF
                     HVAPARPGTPTPTVTLLTSSARPQQAVIGSAIITQIVDQMSFAVTQTAPVVTLKREEV
                     AGRDLRYVDFLTPGILGMTIMQLGISSVAFAFVVDRQRGVIRRIMATPISRRNFLAAH
                     VLERLILAVLQVLILLAVAIVMFKVQIVGSLAVLLLITVLGALLFLSLGFAVTGVVAT
                     ENAAAPVTQLVTLPQMFLSGVFFSRDAAPAFLRPVADYLPLTFLNDALRSVSTSGAGL
                     GDLGPQLLGLVAWIVVSFVLAFRFFRLD"
     gene            complement(5650..6408)
                     /locus_tag="VFV20_08255"
     CDS             complement(5650..6408)
                     /locus_tag="VFV20_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5288381.1"
                     /translation="MTRVVSVRDLQKRYGEIQAVAGVSFDVEQGEIFALLGPNGAGKT
                     TTVEMLEGLRPADAGSAMVAGVDVRADPSGVKHRIGVQLQSSAFPENFLTKEIVDLFA
                     LFYERRVDAMALLRAVGLEDKAKQKSEKLSGGQRQRLSIAVAMVNEPQVLFLDEPTTG
                     LDPQARRNLWELIRDIRKRGTTVFLTTHYLDEAEALCDRVAIMDAGRIIALDSPHALI
                     TQLLARGFTKPMVQQQANLEDVFLDLTGHELREA"
     gene            complement(6413..6883)
                     /locus_tag="VFV20_08260"
     CDS             complement(6413..6883)
                     /locus_tag="VFV20_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288382.1"
                     /translation="MSTATLRGARAVAIVCAVLFFVPTVLSFVYPNAFLYSPHQLVYE
                     RMLGAVLAALAVSLLLSMRDPVRNAGVYAVVGLVAGTLDTSVIYSLVVDGGDVAHWFV
                     QVPLLGAVAAVLVLTYTRLRRPHPVVVRIVVAAVLLLPVALFAHDAAYRAFVRP"
     gene            6933..8606
                     /locus_tag="VFV20_08265"
     CDS             6933..8606
                     /locus_tag="VFV20_08265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU5288383.1"
                     /translation="MSNAAPPSWPCLRCGTRNLATASLCARCGAPRGARGTARRRVRE
                     SGSPGFAAVLSALLPGLGQVYEERWVRGILLLILPMFAFTLVGAFVAIADPLTSFVLR
                     NAPFVIFLVVGTALAFHLYVVADAFAGRMHSLRGRHLIDYAVLGLLTLALIFGYTTIY
                     RQSAPWASLAARLFAPFASSHTPGGTPLDPAAPDWTGSERLNVLLMGVDTREGDKTDQ
                     NTDTMLVLSLDPVNRTASMLSLPRDIYIDRPGTYQGKINAAFAFGGASLVRKVVGDML
                     GIPIHSYTLVNFDAFNRIIDGVGGVIVDAKRPVRDEAYPTADFGVERIDILPGPQLMH
                     GDIALKYARSRHDSNDYSRAKRQQEVIGALRSRLSQPEALRTIPSLMDNVGTSVETDF
                     DPANALPLARTGTGVDSSAITSEVLYPCGGDYPHCELSSNSDNGFYLFPDRRKVLELA
                     AQLFYDPRIRQEAAQVEIQNSGARAGSARDVADRLALRAYGIIGVTDGASAKSAVVLR
                     NTSKRYTADQLARALGNIPIETASGGGSGPDIIVRIGSDFRGFATDLTR"
     gene            complement(8676..9653)
                     /locus_tag="VFV20_08270"
     CDS             complement(8676..9653)
                     /locus_tag="VFV20_08270"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013988116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEU5288384.1"
                     /translation="MVQELARPPKLAKGAAPDSAWAYLDGKFVPIRDAKISVMTHAFN
                     YGTGVFEGIRAYWNADEGQLYGLHFREHYRRMLRSCRIMRLNNPHSAEDLVDITVELL
                     RKCNYKEDCYIRPLTYKSSELIGVRLHNLEDGFTVFAVPFGNYVPIEGGISAQVSSWR
                     RVDDNAIPARSKITGSYVNAALAKTEAEESGFDEAIVLTDDGHVSEGSAANLFLVREG
                     TLLTAPASDNILEGIVRSSIMRIAADEKIPLEVRRIDRTELYLCDEAFLCGTGVQVSP
                     ITSIDRRPVGNGEVGPIARRIARIYFDAVRAKNAKYRDWLTPIYPSPAR"
     gene            9874..10911
                     /locus_tag="VFV20_08275"
     CDS             9874..10911
                     /locus_tag="VFV20_08275"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU5288385.1"
                     /translation="MVHSNARRLWAVLGTALFIAAACGGGTTPAAGSPSASAAASGAA
                     NIEQPKRPVEFVISTGPGGGSDIYARAMQAVIEKQKLSPQPVQPTNKEGGSGAVAFTY
                     VFEKKGDMHYIMITLNSFFTTIITQKLPYKATDFTPVANLALDPFFLWVNEDSPWKTA
                     ADLIKAAQDKELVASGTGSKQEDEVLFRRIEQTAKTKPFKYVPQSGGGAVATALAGHQ
                     GGVEVTVNNPSEGLGLMQQKKVRPLCSFTPDSPAAGPYKGLATCKSQGLAIDDYFVMR
                     AILAPPDLKPAQQAFWVDVFKKVYESAEWKKFMEDNNLQPDFKSGIDFRSFINNYQKL
                     HEDIAKANKWV"
     gene            10955..11404
                     /locus_tag="VFV20_08280"
     CDS             10955..11404
                     /locus_tag="VFV20_08280"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEU5288386.1"
                     /translation="MMVIAAVAMFDSRASALPDNTGIFPGGLGPGFYPFWAAFVIFAS
                     AAWVGYQTLTRPQPAEGPFPERRSITSVLALAAPMGVAVFLIPWIGFYFVTAAYMGFF
                     AAVIGRYKWYWVALITIAFPLAIYLAFESGFRVSLPKSIFYESGLPF"
     gene            11404..12939
                     /locus_tag="VFV20_08285"
     CDS             11404..12939
                     /locus_tag="VFV20_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEU5288387.1"
                     /translation="MEVFGDLLSGFAIAAQPFNLLLLLVGVLLGLVVGVLPGLGGTSG
                     VAILLPVTVFIASSGPGGATSAVIFLAGIYWGALFGGVITSILFNIPGEPWAVALLFD
                     GFPLAKRKGRPGLALVSSFVSSFVGAFIATILLTFLVKVLADFALAFGPAELFAVFIL
                     SFASLVGLGSEFPAKSVMMIAFGLLLAAVGFDTITGTPRLTFGQTQVLSGIGFVPVTI
                     GLFGLGEILASADEAGIGHIERLSARVGFRDILEGFRALAKRIVMVAANATIGFFFGV
                     LPGHGATAGSFLGYGLARQWSKNKAEFGKGEISGIMAPQATADAAAVGSLVPMIALGV
                     PGSPTSAVMMAGLFIWGLQPGPLLFVEHKDFVWGLIASIYLGHLLTFLLCLGTVPLLA
                     RIMRTPYAIITPFIVIISVIGSYALNNSLFDVFITLVFGFIGYWLRKMKYPLAPLVVA
                     IVLGDSTERELRKALIGSGGDPTVFVASPIAAVLLALAALMLAYPFVSRYLRRERAAA
                     RAA"
     gene            12943..14019
                     /locus_tag="VFV20_08290"
     CDS             12943..14019
                     /locus_tag="VFV20_08290"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HEU5288388.1"
                     /translation="MTPLDLVLIVAAFALILFGAETFTDGVEWLGRMLNMSEGATGSI
                     LAAIGTATPETLIPVIAILFTNSVDSDEIGVGAILGAPFMLATLVMLLVGVTAFVLRK
                     KRGRDTLRVDASHATRDLTFFLVFYTLALLLALAPADLHFVKGYVGWIFLPAYFVYLY
                     LVLRSSKKSAVDVEEAREEEEAFGELDFAAYLRRIGVAIAPTRPPFWLVLTQTLVAFG
                     AIVLGARFFADFVEDFSHAMGFNTLLVALILAPLATELPEAANSLIWTKDGKDVIALG
                     NVAGAMVFQSTIPVTIGVQLTPWQLGQFGTIAAVFAIASGALIWLQLRLRAKENSLPV
                     SALMLGGSLYVVFIGYVVWSVVAG"
     gene            14165..15181
                     /locus_tag="VFV20_08295"
     CDS             14165..15181
                     /locus_tag="VFV20_08295"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5288389.1"
                     /translation="MKRLLALAFIATLVVTACGTFGIGTSGLKIGMVTDIGQLEDKSF
                     NEFSWKGVQDGAKAVGGTAQVIVTKDIADYKQNIQQFVDQKYDVIVTVGFLIGTDTLA
                     MAQKYPDIQFFGIDQFICVPKDANDKECKGELPKNYQGLIFNEAQAGYLAGIVAGTIT
                     KSGKIGAVGGRSDVPPVVSYIKGYENGAKSVKSNVSVVVNYTEDFNAPDKGEASAKTM
                     IGQGVDVVFQVAGLTGAGALRAACSAKIFGIGVDVDQYLSLPDAKGCIITSAEKHLQN
                     AAGEAIKRFKDKGKQSGNFVNDATNNGIGASPLRNLSPVPAGLEEKLKQATEDMKSGK
                     LKPV"
     gene            15195..16760
                     /locus_tag="VFV20_08300"
     CDS             15195..16760
                     /locus_tag="VFV20_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5288390.1"
                     /translation="MGQQVTDGPTAGSARPVLAMRGITKRFGTGTLANDSIDLDLRHG
                     EIHALLGENGAGKTTLMNILYGLVSPDAGEILIDGEPVTIRDPADAIARGIGMVHQHF
                     MLVPVLTVAENVILGQEITTAGQILDLREASRRIRELAARLGFDVDPAARVSRLSVGQ
                     QQRVEILKAVYRQARILVLDEPTAVLTPQETREIFAVLRKLRDDGASIVFISHKLDEA
                     HDIADRITVIRRGKVVGSRRPEETNEQELADLMVGRSVSLRVERGTSHAGSVALEITG
                     LRANDDRRHEVVRGVDITVRSGEIVGIAGVAGNGQDELIECLIGLRRPTAGHIRLNGI
                     DMARATVDRRRDLGFAFVPADRHRYGLVLPYPVRDNLVLTRYAERPYVGGLGGFIRQN
                     AAIDEEARRLIEQFDIRTPSARDAVSTLSGGNQQKVIVAREFRGDLTVMVLDQPTRGL
                     DVGSIEFIHKQVIARRDAGVGVLLVSAELDEILDLSDRVLVMYRGQIVGEFPAAEAQR
                     ERIGLLMATGAAA"
     gene            16757..17899
                     /locus_tag="VFV20_08305"
     CDS             16757..17899
                     /locus_tag="VFV20_08305"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5288391.1"
                     /translation="MRLRARETWRVVSIPLYAIVLAFIVAALVVIVSSVFTPTGFDPL
                     LPLVAYLQLFAGAFGNVNGVANTLVAAAPLMFGGLAVGLGLKAGLFNIGVAGQFLVGA
                     FASAVVGAQLSAAPTAIGMPLAVLAGALAGAAYGFIPGFLKARTGAHEVVTTIMLNNA
                     AGLLLTWAVNDLVRAPGFTFPRTADIGLSALPVLAGRNLHLGVLLAFAAIFVVRWVLD
                     RTTLGFEIRTVGLNPDAARYAGMRPVFITALTMTLSGLLAGLAGAIVMLGVIGFYAPG
                     ITASVGFDSIAVALLGRSDPVGILFAAILFGAFRAGAPLMQITTSVPIEVIDIIQALV
                     ILFLAADLIVRRVFRVRAAKPEIAELQTVTKTWGGPTPSTPAGSAD"
BASE COUNT         2796 a         6290 c         5988 g         2907 t
ORIGIN      
        1 tctgataggc ggccagcgaa tcgcgctccg acttcgactt ccagaagcgc gcccggttcc
       61 gccggacgta ccacgtggac aggtcgtccc agaacgcctc catccgcagg cacgccgtct
      121 gcgagtcgta ctgatccagc gcggcgcgca cgtcgcggat gaggcgatgc agcgtcgaga
      181 gcagccaccg gtcgatgagc ggacgctccg cgatggcgac cgccggcgcc gccggatcga
      241 accgatcgag gttcgcgtac gtgacgaaga acgcgtacgt gttccagagc gtgagcagcc
      301 ggcgcttcac gtcgccgagc acgtgatagc cgaagagcac cttctcggcg ggatcgtggc
      361 gcgagtagaa ccagcgcacg acgtccgagc cggcgcgctc ggccgcctgc gggaagtcga
      421 tgaagttgtc gccggtcttg tggaactcct cgccgttctc gtcgtacacg aactcgttcg
      481 agagcaccgt gtcgtacggc gcgcggccgg caagcaccgt gctgaagaag agctgcgagt
      541 agtaccagag ccggatctgc tccacgttct cggtgatgaa ctcggccggg aaccacctct
      601 tccactgctc tttgtcggtg agccagttca gcgtcgagaa gggcacgatg cccgcgtcga
      661 gccacacgtc gccggtctcg cgcacgcgcg tgctctccgc cttgcacgtc gggcacgcga
      721 cgatcacgtt gtcgatccac ggccggtgga gctcctgcag ctgctcgagc ccgcggacgg
      781 cccgctcgcg cagctcctgc tcgctcccga ggatgaagaa gtgcccgttc gtgcagttgt
      841 agaagggcag cggcagcccc cagtagcgct tgcggctgat gttccagtcg cccatgttgt
      901 cgagccagtt ctgcatccgg tcgccggtgt gcggcgggag ccagcgcacc ttcgcggccg
      961 cgtccttcag cttgggacgg agctcgtcca tggagatgaa ccactcgtcc gcgacgcgga
     1021 agacgatctc ctctttgcag cgccagcaga ccgggtagct gtgcgtgtag ggcacctcgc
     1081 ggaacagccg cccgcgcttc cgcaggtcgt cggcgatctc gtgggcgacg tcgcgcgcct
     1141 gccgcccctc gagccacccg aatccggaaa cgaagttcgc gttctcgtcg atcggcacga
     1201 tcaccggaag accgttcggc ttcgcgagcg cgaagtcctc ggcgccgcag cccggcgcga
     1261 tgtggacgat gcccgtgccc tccgcctcgc cgacctcgtc ccacgcgatc acggtatggg
     1321 cgggaccgga cgtggcgccg acgggcagct cgtcgaacgg acccgtgtag cgaagcccga
     1381 ggaggtcgcg ccccttgacc gtgccgagga gctcggcgtt gtgccacttc gcctgcgcgt
     1441 acacgtgggc accgaggatg aggagctccg gcttggtggc gccatccgcg accacacgca
     1501 ccttcgcgta gtcgagatcg ggatgtaccg ccagcgcgac gttggccggc agcgtccagg
     1561 gggtcgtcgt ccacgccagg aacgaggctc gctgcgtcgc caggtcggcc cccctgccat
     1621 cggggaggac cgggaagcgg acatacagcg aggtgtgcgt catctccttg tacgacccgg
     1681 tcatctcgtg ctccgagagc gccgtcccgc agcgcgcgca ccacggcatc gctcggtggc
     1741 cgcggatgag ccagcccttc tcgtggcaca gcttcagcac gtgccagatg tgcgagatgt
     1801 tgttgtccga gtacgtgaag tacgagttgt cccagtccat ccactggccg aggcgcctcg
     1861 actgcccggt gatcgccgcg gcgctgcggt cgacgcgcgc gcggcacgcg cgcgagaact
     1921 tgtcgatgcc gtaggtctcg atgtcgcgct tgctgttgag gttgagctcc cgctccacct
     1981 gcacctcgac ccagagtccc tggcagtcga agccgttctg gtagcgctgg tcgaacccct
     2041 gcatggcctt gtagcgctgg tagatgtcct tgtaggtgcg tccccaggcg tgatggacgc
     2101 ccatcgcctc gatgttggcg gtgatcgggc cgtcgatgaa cgagtagcgc ggtccgccgg
     2161 cgttcttctt ccggagcgtc tcgaaactct tctcccgctc ccagaggtcg agcacgccgt
     2221 gttcgagctc gggcaggtcg aagctcgacc cgaccgccgc gaatgtcctg ctcaaggcct
     2281 cgttgtcact cggcaatgga ctcgctcctc ggggcaacgc ccctcgtcac tcgcttccgt
     2341 cgaatcgctc gtcgctcgac tccctttgtg aaacagaaac gccgtcccgc tctcgggacg
     2401 gcgcatgccg tggtaccacc cgagttcgcg cggcacacgc gtgccgcacg cactccttcg
     2461 ccgacgtcca gcaacgtccg ccgcttgata acggagtggc ttcctccggc cggttctaat
     2521 cgcccgaacg ggctttcttc cggcggcatc ggggtcgatc gcccctcgtc gcctgtcgcg
     2581 ctcagtgtac gggaacgacc tcgctcgcca gcgcgacgag ccgcccgatc gcggtctcgg
     2641 cacgcgctcc cgacgacgcg agcgcgcgca gctccccgct ccggcggagc gactccccca
     2701 ggacgacccc ggcaggcatg gcctcgcgcg cggcgccgac gatctccgtg atccgccgtc
     2761 caccgacggt gagcaggtac tcgatgccca tccccttgag cgcgacctcg aggtcgtcgc
     2821 gcggaccggc gaatgagatc cctttcgcgc gcaggtacgg cgtggcgacg aagagcgagg
     2881 cactgcccgc ccatgggtcg cgcgacgcgt aacgcaccgc gcgcacgtcc gccatggcga
     2941 tcgctccggc acagagcggg cacggctcga cggtggtgta gacggtcagc gaccgcggtt
     3001 cgccgtccca cgtggacgcg tcgagcagcg cgttgacctc ggcgtgcgcg agccggtgcc
     3061 cggcgagctg cggcgcgggc gggctcgact cccgtatgcg gttgcggccc cggccgacga
     3121 cgcgacccgc cgcgtccgcc acgcacgcac cgatggggtt ggatccggcg cgataggcgt
     3181 cccaggcctc ctccagacag gcgcgccacg gcgccgcgag cgcgtcccac cagcccttga
     3241 cgtacatatg tgatgacgat atatatgctg tcaacatata tcggaggagt cgagatggcg
     3301 aagaccgcgc ggccgcagcc caaagagcgt ggagaggtcg cctgcctggc gcggccggcc
     3361 ttcgagaccg cgctcgccgc gagcctcgag cgcgagccgg tcgcgacact cctcgacgtc
     3421 gatctcgacg ggctgctcga cctgaacgag cgcggcggcc atgaggcggg cgacagggcc
     3481 atcgccgccg tgacgcaggc gctcgcgaag gtggcgaagg cggagggctg gatcctcgga
     3541 cgcatcggcg gcgacgagtt cgcgctgctc gcgccgggcg tcgccctcga gcccgcgttc
     3601 ctccgggccg atcagatccg tcgcgatgcc gacgcggcgc tcgcgaaggc gctgcccggc
     3661 gcccatcgct gcaccgtcac catcggcgtc gcgaacacgc cccgcgacac caagcagcgc
     3721 ggaaaggacg cggcgaacga gctcctccgc cgcgccgatc tggcgctcca cgccgccaag
     3781 gagcagggcg gcgacaccgt cgggctcaca cccgcggacg acatggtcct caagtcctcg
     3841 tactacagcg gcgcgcagct cggtcgcctg aagggcctcg ccgagcgcat gaagaagaag
     3901 gaggcggtgc tgctccgcga ggcgctcgac gacctgctgc gcaagtacca gcgctcgtag
     3961 gctgtcgccg tggacctgcc gccgaccgtc gtgcgcgagc tggacgatct cgcgcgccgc
     4021 ttcggcgagc cgctgcgcct tgctgcgccg atcgacgact ccttcacgga cccgatctgg
     4081 aagcgggacc gcttcggtga ggtctgcatg gtcgtacgcc gccccagcgg gaagctgctg
     4141 ctgtccatca agaccttcta tccccgcggc gcgcaccgcc tgccgacggg cggcatccac
     4201 cacggcgagg gcgtcttcga cgcgctcgtg cgcgagacgc acgaggagac cggcctcgtg
     4261 accgccccgc ggcgctttct cgcacgcatc gcctacgacc cgctctcgac gccgggcgcc
     4321 ccgccggtct tccacacctt cgcgttcctc ctcgacgagg tggccggcac gctcggggcg
     4381 atcgacacga gcgagcgcat cgaggagtgg aaggagatcg acgtcgccga ccttccgcgg
     4441 gtcgcccaca cgctggacca cctcacggcg caggggaccg aggacatcgg tggcgactgg
     4501 gcggcctggg gcaggttccg cgcggtcgtg caccgcgtcg tgcacgaggc gctgtccgtc
     4561 tagtccaggc ggaagaaccg gaacgcgagg acgaacgaga ccacgatcca ggcgacgaga
     4621 ccgaggagct gcgggccgag gtcgcccaga cccgcgcccg acgtgctcac gctgcggagc
     4681 gcgtcgttga ggaacgtgag cggcaggtag tcggccaccg gacgaaggaa cgccggcgcg
     4741 gcgtcgcgtg agaagaagac gcccgacagg aacatctgcg gcagcgtcac gagctgcgtc
     4801 accggcgcgg cggcattctc ggtcgcgacg acgccggtga ccgcgaagcc gagcgagagg
     4861 aacagcagcg caccgagcac ggtgatgagg agcaggaccg cgagactgcc cacgatctgc
     4921 accttgaaca tgacgatcgc cacggcgagc aggatcagca cctggaggac ggcgaggatc
     4981 agccgttcca gcacgtgcgc ggcgaggaaa ttccgtcgtg agatcggcgt ggccatgatc
     5041 cggcggatga cgccgcgctg acggtccacc acgaacgcga acgcgacgct cgagatgccc
     5101 agctgcatga tcgtcatccc gaggatgccc ggcgtaagga agtcgacgta gcggaggtcc
     5161 cggccggcga cctcctcgcg cttcagcgtc accaccggcg ccgtctgcgt caccgcgaag
     5221 gacatctggt cgacgatctg cgtgatgatc gcggagccga tgaccgcctg ctggggccgg
     5281 gcgctgctcg tcagcagcgt gaccgtgggc gtcggcgtgc cggggcgcgc gggcgcgacg
     5341 tggaagtccg cggggaggac gagcacgagg tcgcggtcgc ccgcttccag cttcccgctc
     5401 tcgtcggccc gggtgccgct cgtgatcttg aacgtctcga tcccgcggag cgtcgcgatg
     5461 aagcgctcgc tgtccgcgtt cctcgcctcg tcggcgatgc cgaggttcac gtggccgacc
     5521 gcgccgaggt ccaggatgcc gaacagcatc atgaagatga gcgggaagaa gaatccgaag
     5581 aagagcgcgg ggcggtcccg cgtgtacgag cgcagcagca tgagcgtgta gcgaaggatc
     5641 tgccccacct caggcctccc gcagctcgtg gccggtgaga tcgaggaaga cgtcctcgag
     5701 gttcgcctgc tgctgcacca tcggcttggt gaagccgcgc gcgagcagct gcgtgatgag
     5761 cgcgtgcggc gagtccagcg cgatgatgcg gcccgcgtcc atgatggcca cgcggtcgca
     5821 gagcgcctcc gcctcgtcga ggtagtgcgt ggtgaggaag acggtcgtgc ctcgtttccg
     5881 gatgtcgcgg atgagctccc agaggttccg ccgcgcctgg gggtccaggc cggtggtcgg
     5941 ctcgtcgagg aagaggacct gcggctcgtt caccatggcc accgcgatcg agagacgctg
     6001 gcgctggccg cccgagagct tctcggactt ctgcttcgcc ttgtcctcga ggccgacggc
     6061 acgcagcagc gccatcgcgt ccaccctccg ctcgtagaag agcgcgaaga ggtcgacgat
     6121 ctccttcgtg aggaagttct cggggaacgc gctcgactgc agctggacgc cgatgcgatg
     6181 cttcacgccc gacgggtcgg cgcgcacgtc gacacccgcg accatcgcgc tcccggcgtc
     6241 ggccgggcgc agcccctcga gcatctcgac ggtggtggtc ttccccgccc cgttcggtcc
     6301 gagcagcgcg aagatctcgc cctgctcgac gtcgaaggag acgcccgcga ccgcttggat
     6361 ctcgccgtag cgcttctgga ggtcacgcac cgagacgacc ctggtcatgg gatcagggac
     6421 gcacgaacgc gcggtacgcg gcgtcatgcg cgaagagggc gacaggaagc agcaggaccg
     6481 ccgcgacgac gatgcgcacg acgacggggt gcggcctccg gagccgggtg taggtgagga
     6541 cgaggaccgc ggcgacggcg cccagcagcg gcacctggac gaaccagtgc gcgacgtcgc
     6601 cgccgtcgac gaccagcgag tagatcaccg acgtgtcgag cgtcccggcg accagaccga
     6661 cgaccgcgta gacgccggca ttgcggacgg gatctcgcat cgaaaggagg agcgacaccg
     6721 cgagcgccgc gaggaccgcg ccgagcatgc gctcgtacac gagctgatgc ggggagtaga
     6781 ggaacgcgtt cgggtatacg aacgacagca ccgtcgggac gaagaagagg accgcgcaga
     6841 cgatggcgac ggcgcgcgca ccgcgcagcg tggccgtgct cacatgacgg ctccgttcca
     6901 ctagcgtccg agtctcgtac gctagtcgtt ccatgtcgaa tgccgcaccc ccgtcgtggc
     6961 cgtgcctgcg ctgcgggaca cgcaacctcg ccacggcatc gctctgcgcg cgctgcggcg
     7021 cgccgcgcgg cgcgcgcggg acggcccgtc gccgcgtgcg cgagagcgga tcgccgggct
     7081 tcgcggccgt gctctcagcg ctgttgcccg gcctcggtca ggtgtacgag gagcgctggg
     7141 tccgcgggat cctgctgctc atcctcccga tgttcgcctt cacgctcgtc ggcgcgttcg
     7201 tggcgatcgc cgacccgctg acctcgttcg tcctccggaa cgcgcccttc gtgatcttcc
     7261 tggtcgtcgg tacggcgctc gcattccacc tgtatgtcgt ggccgacgcg ttcgcggggc
     7321 gcatgcactc gctgcgtgga cgccatctca tcgactacgc cgtgctgggg ctcctcacgc
     7381 tcgcgctcat cttcggctac acgacgatct accggcagtc agcgccgtgg gcctcgctcg
     7441 ccgcccggct cttcgcgccg ttcgcgtcgt cacacacgcc cgggggtaca ccgctggacc
     7501 cggccgcgcc cgactggacc ggcagcgagc ggctgaacgt cctgctgatg ggtgtcgaca
     7561 cccgcgaggg cgacaagacc gatcagaaca cggacacgat gctcgtcctc tcgctcgacc
     7621 cggtgaaccg gacggcctcg atgctctctc tgccacgcga catctacatc gaccggcccg
     7681 gcacctacca gggcaagatc aacgcggcgt tcgcgttcgg cggcgcatcg ctcgtgcgaa
     7741 aggtcgtcgg cgacatgctg ggcatcccga tccactcgta cacgctcgtc aacttcgatg
     7801 cgttcaaccg gatcatcgac ggcgtcggcg gcgtcatcgt cgacgcgaag cggccggtca
     7861 gggacgaggc gtatcccacg gcggacttcg gcgtcgaacg gatcgacatc cttcccggcc
     7921 cacagctcat gcatggcgac atagcgctga agtacgcacg atcgcgtcac gactcgaacg
     7981 actacagcag ggcgaagcgg cagcaggagg tcatcggcgc gctgcggtca cggctgtcgc
     8041 agcccgaggc cctgcgcacg atcccctcgc tcatggacaa cgtcggcacc tctgtcgaga
     8101 ccgacttcga tcccgcgaac gcgctaccgc tcgcgcgcac cggcacgggg gtcgattcct
     8161 cggcgatcac gagcgaggtg ctgtacccgt gcggcggcga ttacccgcac tgcgagctca
     8221 gctcgaacag cgacaacggc ttctacctgt tcccggaccg gaggaaggtc ctggagctcg
     8281 ccgcgcagct cttctacgac ccccgcatcc gccaggaggc cgcgcaggtg gagatccaga
     8341 acagcggcgc gcgcgccggc tcggcacggg acgtcgccga tcgcctggcg ctgcgcgcgt
     8401 acggcatcat cggcgtgacc gacggcgcga gcgcgaagtc ggcggtcgtg ctgcgcaaca
     8461 cctcgaagcg ctacaccgcc gatcagctcg cccgcgcgct cgggaacatc ccgatcgaga
     8521 ccgcgagcgg cggcggctcc ggccccgaca tcatcgtgcg catcggctcc gacttccgcg
     8581 gcttcgcgac cgatctgacg cgctgacggc gagaactcgt cgcattcagc ggcgagccac
     8641 ggtggcgtcc gctcctcgcc gccgcatctg acccgttacc gcgccggcga cggatagatc
     8701 ggcgtcagcc agtcgcggta cttggcgttc ttcgcgcgca ccgcgtcgaa gtagatacgc
     8761 gcgatgcgtc gagcgatcgg ccccacctcg ccgttgccga cgggccggcg gtcgatgctc
     8821 gtgatcggcg agacctgcac gccggtaccg cagaggaacg cctcgtcgca gaggtaaagc
     8881 tcggtccgat cgatgcgccg cacctcgagc gggatcttct cgtccgcggc gatgcgcatg
     8941 atgctcgacc ggacgatgcc ctcgaggatg ttgtcggatg ccggcgcggt gagcagcgtg
     9001 ccctcgcgca cgaggaacag gttcgccgcg ctgccctcgg agacatggcc gtcgtcggtc
     9061 agcacgatcg cctcgtcgaa cccggactct tccgcctcgg tcttcgcgag cgccgcgttc
     9121 acgtaggacc cggtgatctt gctgcgcgcc gggatggcgt tgtcgtcgac gcgtctccac
     9181 gacgagacct gcgccgagat gccgccctcg atcggcacgt agttgccgaa cggcacggcg
     9241 aacacggtga agccgtcctc gaggttgtgg agacggacgc cgatgagctc gctcgacttg
     9301 tacgtgagcg gccggatgta gcagtcctct ttgtagttgc acttccgcag cagctcgacg
     9361 gtgatgtcga cgaggtcctc cgcggaatgg gggttgttca ggcgcatgat ccggcacgag
     9421 cgaagcatcc ggcggtagtg ctcgcggaag tggagcccgt agagctgccc ttcgtcggca
     9481 ttccaatagg cgcgaattcc ctcgaagacg ccggtcccgt agttgaacgc gtgcgtcatc
     9541 acgctgatct tggcgtcgcg gatggggacg aacttcccgt cgaggtatgc ccaggccgag
     9601 tcgggcgccg cgcctttggc cagcttcggg ggccgcgcta gctcctgcac catgccagtc
     9661 tccttagtgg gtcgcttcga gcgacccccc ttcgagcggc cgaccgcacg ccccatgccg
     9721 acccctctca gcattctgac accggcgccg acagggcccc atgaccgccc gggacttgcc
     9781 gagcgtagtc cgcccgctac gatgccgctg gtcagaccgg agaggatccg accgaacgcg
     9841 tcaggcgtac gagttccgtg gaggtgcaga ggcatggtgc actcgaacgc aaggcgactc
     9901 tgggcggtgc tcggcaccgc gctcttcatc gcggcggcat gcgggggcgg gacgacgccg
     9961 gccgcgggat cgccaagcgc cagcgcggcc gcgtcgggcg cggcgaacat cgagcagccg
    10021 aagcgtcccg tcgagttcgt catcagcacg ggtcccggtg gagggtccga catctacgcg
    10081 cgtgcgatgc aggcggtcat cgagaagcag aagctctcgc cgcagccggt ccagcccacg
    10141 aacaaggagg gcggctcggg tgcggtcgcg ttcacctacg tcttcgagaa gaagggtgac
    10201 atgcactaca tcatgatcac cctcaactcc ttcttcacga cgatcatcac gcagaagctc
    10261 ccgtacaagg ccaccgactt caccccggtc gcgaacctcg cgctcgaccc cttcttcctg
    10321 tgggtgaacg aggacagtcc gtggaagacc gcggccgacc tcatcaaggc ggcgcaggac
    10381 aaggagctgg tcgcgtcggg caccggctcg aagcaggagg acgaggtcct cttccggcgc
    10441 atcgagcaga ccgcgaagac caagccgttc aagtacgtgc cgcagtcggg cggcggtgcg
    10501 gtcgcgacgg cgctcgccgg ccaccagggc ggtgtcgagg tgaccgtcaa caacccgagc
    10561 gagggcctcg gtctcatgca gcagaagaag gtccgtccgc tctgctcgtt cacgccggac
    10621 tcgccggccg ccggcccgta caagggcctc gcgacatgca agtcgcaggg actcgcgatc
    10681 gacgactact tcgtgatgcg cgcgatcctc gccccgccgg acctcaagcc ggcgcagcag
    10741 gccttctggg tggacgtctt caagaaggtg tacgagtcgg ccgagtggaa gaagttcatg
    10801 gaggacaaca acctccagcc cgacttcaag tccggtatcg acttccgcag cttcatcaac
    10861 aactaccaga agctgcacga ggacatcgca aaggccaaca agtgggtcta gatctcatca
    10921 ggtgaggccg tatcaagctg ggacggcggc ggtcatgatg gtgatcgccg ccgtcgccat
    10981 gttcgacagt cgcgcgagcg cgctgcccga caacaccggc atcttccccg gcgggctcgg
    11041 cccgggtttc tatccgttct gggccgcgtt cgtgatcttc gcgtcggccg catgggtcgg
    11101 ataccagacg ctgacccggc cgcagcccgc ggaggggccg ttccctgagc ggcgcagcat
    11161 cacgtcggtg ctcgcgctgg cggcacccat gggggtcgcc gtgttcctca tcccctggat
    11221 cggcttctac ttcgtgaccg cggcgtacat gggcttcttc gcggcggtca tcggccgcta
    11281 caagtggtac tgggtggcgc tcatcacgat cgccttcccg ctggcgatct atctggcgtt
    11341 cgagagcggg ttccgcgtgt cgctgccgaa gagcatcttc tacgagtccg gtctgccgtt
    11401 ctgatggaag tcttcggcga cctcctcagc ggcttcgcga tcgcggcgca gccgttcaac
    11461 ctcctgctgc tcctcgtcgg cgtgctcctc gggctcgtcg tcggcgtgct ccccggcctg
    11521 ggcggcacga gcggtgtggc gatcctgctg ccggtcaccg tcttcatcgc gtcgagcggc
    11581 cccggcggtg ccacgagcgc ggtgatcttc ctcgccggca tctactgggg cgcgctcttc
    11641 ggcggtgtca tcacgtcgat cctcttcaac atccccggcg agccgtgggc cgtggcgctg
    11701 ctcttcgacg gcttcccgct cgccaagcgc aagggcaggc ccgggctcgc gctcgtctcg
    11761 tccttcgttt cgtcgttcgt cggcgcgttc atcgcgacca tcctgctcac ctttctggtg
    11821 aaggtcctcg cggacttcgc gctcgccttc gggccggccg agctcttcgc cgtcttcatc
    11881 ctgtcattcg catcgctcgt cggactcggc tcggagttcc ccgcgaagag cgtgatgatg
    11941 atcgcgttcg gactgctgct cgcggcggtc ggcttcgaca cgatcaccgg cacgccgcgt
    12001 ctcaccttcg ggcagacgca ggtcctctcg ggcatcggtt tcgtgccggt gacgatcggg
    12061 ctcttcgggc tcggcgagat cctcgcgtcc gccgacgaag cgggtatcgg gcacatcgaa
    12121 cgcctctccg cgcgcgtcgg cttccgcgac atcctcgagg gcttccgtgc gctcgccaag
    12181 cgcatcgtca tggtcgcggc gaacgcgacc atcggcttct tcttcggcgt cctgcccggg
    12241 cacggcgcca cggccgggtc gttcctcggc tacggcctcg cacggcagtg gtcgaagaac
    12301 aaggcggagt tcggcaaggg cgagatctcc gggatcatgg cgccgcaggc gaccgcggac
    12361 gccgcggcgg tcggttcgct cgtgccgatg atcgcgctcg gcgtgcccgg atcgccgacc
    12421 tcggccgtga tgatggccgg cctcttcatc tgggggttgc agccgggccc gctgctcttc
    12481 gtcgagcaca aggacttcgt ctggggcctc atcgcgagca tctacctcgg ccacctgttg
    12541 acgttcctgc tctgcctggg cacggtgccg ctgctcgccc gcatcatgcg cacgccgtac
    12601 gcgatcatca cgccgttcat cgtgatcatc tcggtgatcg ggtcgtacgc gctgaacaac
    12661 agcctcttcg acgtgttcat cacgctcgtc ttcggtttca tcggatactg gctacggaag
    12721 atgaagtacc cgctcgcgcc gctcgtcgtc gcgatcgtgc tcggggactc gacggagcgc
    12781 gagctgcgca aggcgctcat cggcagcggc ggcgatccga ccgtgttcgt ggcgagcccg
    12841 atcgccgccg tcctgctggc gctcgccgcg ctcatgctcg cctacccctt cgtctcgcgg
    12901 tacctccgcc gcgagcgcgc cgccgcccgc gccgcctagc cggtgacccc gctcgacctc
    12961 gtcctcatcg tcgcggcgtt cgcgctcatc ctcttcggag cggagacgtt caccgacggc
    13021 gtcgagtggc tcggtcgcat gctcaacatg agcgagggcg cgactgggag catcctcgcg
    13081 gcgatcggca cggccacacc ggagaccctc atcccggtga tcgcgatcct cttcacgaac
    13141 tccgttgact cggacgagat cggtgtcggc gcgatcctcg gcgcaccgtt catgctggcg
    13201 acgctggtga tgctcctcgt cggcgtcacg gccttcgtcc tccgcaagaa gcgcggacgc
    13261 gacacgctgc gcgtcgacgc gagccacgcg acccgcgacc tcacgttctt cctcgtcttc
    13321 tacacgctcg cgctgctcct ggcgctggcg cccgcggacc tccacttcgt gaagggctat
    13381 gtcggctgga tcttcctgcc ggcctacttc gtgtacctgt acctcgtgct acgtagttcg
    13441 aagaagagcg cggtcgacgt cgaggaggca cgagaggagg aggaggcgtt cggcgagctc
    13501 gacttcgccg cctacctgcg gcgcatcggc gtggccatcg ctcccacgcg ccctccgttc
    13561 tggctcgtgc tgacgcagac gctcgtggcg ttcggcgcga tcgtcctggg cgcgcgcttc
    13621 ttcgccgact tcgtcgagga cttctcgcac gcgatggggt tcaacacgct cctcgtcgcg
    13681 ctcatcctgg cgccgctcgc gaccgagctg ccggaggcgg cgaacagcct catctggacc
    13741 aaggatggga aggacgtcat cgcgctcggg aacgtcgcgg gcgcgatggt gttccagtcc
    13801 acgatccccg tgaccatcgg cgtgcagctc acgccatggc agctcgggca gttcggcacg
    13861 atcgccgcgg tcttcgccat cgcgtccgga gcgctcatct ggctgcagct gcggctgcga
    13921 gcgaaggaga actcgctgcc ggtctccgcc ctgatgctcg gcggcagcct gtacgtcgtg
    13981 ttcatcggat acgtcgtttg gagcgtcgtc gcaggctaac tgcccgtcgc atcgtgcggt
    14041 cagaccacac acccgacccg agatctgccc cacgacgggg atgaagacgc atcactagaa
    14101 tccctcagcg acgcgggtgg aggcttccgc cgcccgccca gatgagggag gtacggcaga
    14161 gctcatgaag cgtctccttg cactcgcgtt catcgccacg ctcgtcgtca cggcgtgcgg
    14221 caccttcggg atcggtacat ctggcctcaa gatcgggatg gtgaccgaca tcggccagct
    14281 cgaggacaag tccttcaacg agttctcgtg gaagggcgtc caggacggcg cgaaggccgt
    14341 cggtggcacc gcgcaggtca tcgtcaccaa ggacatcgcc gactacaagc agaacatcca
    14401 gcagttcgtg gatcagaagt acgacgtcat cgtgaccgtc ggcttcctca ttgggacgga
    14461 cacgctcgcg atggcgcaga agtacccgga catccagttc ttcggcatcg accagttcat
    14521 ctgcgtcccc aaggacgcga atgacaagga atgcaagggc gagctgccga agaactacca
    14581 gggcctgatc ttcaacgaag cacaggccgg ctacctcgcg ggtatcgtcg ccggcaccat
    14641 cacgaaaagc ggcaagatcg gcgcggtggg cggccgttcg gacgtcccgc ccgtcgtgag
    14701 ctacatcaag ggctatgaga acggcgcgaa gtccgtgaag tcgaacgtct cggtcgtcgt
    14761 caactacaca gaggacttca acgcccccga caagggcgag gcctcggcga agacgatgat
    14821 cggccagggc gtcgacgtgg tcttccaggt cgccggtctc accggcgccg gcgcgctccg
    14881 cgctgcctgc agcgcgaaga tcttcggcat cggcgtcgac gtggaccagt acctctcgct
    14941 ccccgacgcg aagggctgca tcatcacgag cgcggagaag cacttgcaga acgccgccgg
    15001 tgaggcgatc aagcgcttca aggacaaggg caagcagagc ggcaacttcg tcaacgacgc
    15061 gacgaacaac ggcatcggcg catcgccgct ccgcaacctc tcgccggtcc cggcggggct
    15121 cgaggaaaag ctcaagcagg ccaccgaaga catgaagtcc gggaagctca agccggtctg
    15181 atcgaccgca tcacgtggga cagcaggtga cggacgggcc gaccgcgggg tcggcccgtc
    15241 ccgtcctcgc gatgcgcggc atcaccaagc gcttcggcac ggggacgctc gcgaacgatt
    15301 ccatcgatct cgacctgcgc cacggcgaga tccacgcgct gctcggcgag aacggtgccg
    15361 gcaagacgac cctcatgaac atcctctacg ggctcgtgtc gcccgacgcc ggcgagatcc
    15421 tcatcgacgg cgagccggtg accatccgcg atcccgccga cgccatcgca cgcggcatcg
    15481 gcatggtcca ccagcatttc atgctcgtcc ctgttctcac cgtcgcagag aacgtgatcc
    15541 tcgggcagga gatcacgacg gccggccaga tcctggacct ccgtgaggcg tcgcggcgga
    15601 tccgcgagct cgcggcacgc ctcggattcg acgtcgaccc ggcggcccgc gtgtctcgcc
    15661 tgtcggtggg ccagcagcag cgggtcgaga tcctgaaggc cgtctaccgt caggcgcgga
    15721 tcctcgttct cgacgagccc accgccgtcc tcacgccaca ggagacccgc gagatcttcg
    15781 cggtcctccg gaagctgcgc gatgacggcg cgagcatcgt cttcatcagc cacaagctgg
    15841 acgaggcgca cgacatcgcc gaccgcatca ccgtcatccg gcgcggcaag gtcgtcggga
    15901 gtcggcgccc ggaggagacg aacgagcagg agctcgcgga cctcatggtc ggccgcagcg
    15961 tgtcgctcag ggtcgagcgc ggaacgtcgc acgccggctc ggtggcgctg gagatcaccg
    16021 ggctgcgcgc gaacgacgat cggcgtcacg aggtcgtacg cggcgtcgac atcaccgtgc
    16081 gttcaggtga gatcgtcggc atcgccggcg tcgcggggaa cggccaggac gagctgatcg
    16141 agtgcctcat cggcctacgc cgcccgacgg cgggtcacat ccgcctcaac gggatcgata
    16201 tggcgcgcgc cacggtggat cgccggcgcg acctgggctt cgcgttcgtg ccggcggacc
    16261 ggcaccgcta cgggctcgtc ctcccctatc cggtgcgcga caacctcgtg ctgacccggt
    16321 acgcggagcg cccgtacgtc ggcggtctcg gcggcttcat ccgccagaac gccgcgatcg
    16381 acgaggaggc gcgccgcctc atcgagcagt tcgacatccg gaccccgtcg gcgcgcgacg
    16441 ccgtgagcac gctctcgggc ggcaaccagc agaaggtcat cgtcgcgagg gagttccgcg
    16501 gtgacctcac ggtcatggtc ctcgaccagc cgacccgcgg cctcgacgtc ggcagcatcg
    16561 aattcatcca caaacaggtc atcgcgcgcc gcgacgcggg cgtcggcgtg ctgctcgtgt
    16621 cggcggagct cgacgagatc ctcgacctct ccgaccgcgt cctcgtgatg taccgcgggc
    16681 agatcgtcgg cgagttcccg gccgccgagg cgcagcggga acgcatcgga ctgctcatgg
    16741 ccacgggagc ggcggcgtga ggctgcgcgc gcgcgagacg tggcgcgtcg tgtcgatccc
    16801 gctctatgcc atcgtcctcg ccttcatcgt cgccgcactc gtcgtcatcg tgtcgagcgt
    16861 gttcacgccg acgggcttcg atccgctcct ccccctcgtg gcctacctcc agctgttcgc
    16921 cggagcgttc gggaacgtca acggcgtcgc gaacaccctc gtcgccgcgg cgccactcat
    16981 gttcggcggc ctcgcggtcg ggctcggcct gaaggccggt ctcttcaaca tcggcgtcgc
    17041 gggacagttc ctcgtcggag cgttcgcctc agcggtggtc ggcgcgcagc tctcggcggc
    17101 gccgacggcg atcggcatgc cgctcgccgt gctcgccgga gcactcgccg gggccgcgta
    17161 cggcttcatc cccggattcc tcaaggcgcg gaccggtgcg cacgaggtcg tgaccaccat
    17221 catgctcaac aacgcggccg ggctcctcct cacctgggcg gtgaacgacc tggtgcgcgc
    17281 gcctggcttc accttcccgc gtaccgccga catcggactg agcgcgctcc cggtgctcgc
    17341 gggacgcaat cttcacctcg gcgtgctcct cgcattcgcg gcgatcttcg tcgtgcgctg
    17401 ggtcctcgat cgcacgacgc tcggcttcga gatccgcacc gtcgggctga atcccgacgc
    17461 ggcgcgctac gcagggatgc gaccggtgtt catcacggcg ctcacgatga cgctctcggg
    17521 cctgctcgcg ggcctcgccg gagccatcgt gatgctcggt gtcatcggct tctacgcgcc
    17581 gggcatcaca gcctcggtcg gcttcgactc catcgcggtc gcgctgctcg gacggtcgga
    17641 tccggtcggc atcctcttcg cggcgatcct ctttggagcg ttccgcgccg gcgcaccgct
    17701 catgcagatc accacctcgg tacccatcga ggtcatcgac atcatccagg cgctcgtgat
    17761 cctcttcctc gccgccgatc tcatcgtgcg cagggtgttc cgggtgcgcg cggcgaagcc
    17821 agagatcgcc gagctccaga ccgtcacgaa gacgtggggc gggccgacgc ccagcacccc
    17881 cgcgggctcg gccgactgat ggacatcctc acgatccccc gcctgctctg ggagttcgtg
    17941 gtctacctgt tcaccgtggt ccccgacctc ctgccgatca t
//
LOCUS       DASTZR010000370         3555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_464523, whole genome shotgun sequence.
ACCESSION   DASTZR010000370 DASTZR010000000
VERSION     DASTZR010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464523"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..108
                     /locus_tag="VFV20_08310"
     CDS             <1..108
                     /locus_tag="VFV20_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015864617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEU5288392.1"
                     /translation="EAFRVRGHPVATGEFGAMMSVELVNDGPVTLVLER"
     gene            105..707
                     /locus_tag="VFV20_08315"
     CDS             105..707
                     /locus_tag="VFV20_08315"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015375502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEU5288393.1"
                     /translation="MIPKVVLASASPRRRELLTLVGIEHAVVPSHLDETMHPGEQPGP
                     HARRLAVEKATAIAASHRDAVVIGADTVVVLDGKVLGKPATPEVAREMLRALNGRTHT
                     VITAVAVAYAGRTSEGAEEVSVTFRQLSDDEIDAYVATREGMDKAGAYGIQGFGATIV
                     ERIDGDFFAVMGLPLVRLISLLRDVGLRYRFGNVESAAAE"
     gene            complement(668..1369)
                     /locus_tag="VFV20_08320"
     CDS             complement(668..1369)
                     /locus_tag="VFV20_08320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEU5288394.1"
                     /translation="MNTMFTTHTIPPRAPQHVQDQYFEFLTYMGVPHEPVEWHLGPWE
                     SEREWQREFSARFDRPVASVVVGTSKAAKDWAPERWAEVCDALYADYGLQPVLVGGGS
                     ERERRAERVILERSKHPHFSALGSGLRNLVCILDASALVLAPDTGPLHMAVALDRPVV
                     SLMGFTNPKRSGPYRKFHDLIVDAFGDPGESYDASAKHRAGRMARITPRDVLEKVDVW
                     KARYSAAALSTLPKR"
     gene            complement(1715..2473)
                     /locus_tag="VFV20_08325"
     CDS             complement(1715..2473)
                     /locus_tag="VFV20_08325"
                     /inference="COORDINATES: protein motif:HMM:NF018047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HEU5288395.1"
                     /translation="MRLEIPGYARFRVGRARVTALEEEERSIREALRYGTLYEYAGRH
                     PERRQMKGRGDVFAAPLPGHEESVVVRHAFRGGAPAHVTRDLFLPPTRGLRELLVSQR
                     LRAAGVHTPEVVAYVTYPAGAILRRFDVATREIPNGADLFAWLAATEDEGARDVVLNA
                     AAGAVASLVAAGAHHPDLNIRNVLVAPGPDGGLIGWILDVDRVRFHAAGSPTVRDANI
                     TRLRRSLRKSMRLGLSITDEEILAILDRVQELSE"
     gene            complement(2475..>3555)
                     /locus_tag="VFV20_08330"
     CDS             complement(2475..>3555)
                     /locus_tag="VFV20_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEU5288396.1"
                     /translation="AMFVFTRSLVVIDNLHGTANIVITVQVPVGASDAELRESYAAAV
                     ADADELRARLRAPSPLLPLELGDDREVKGKSSLDKSSFEGAVVRVKEHILAGDAFQVV
                     LTRRIELPATFDTVDLYRALRAINPSPYMYCLTLDEMEIVGSSPELLVRLQDGMTTVR
                     PIAGTRPRPRPGATPGYDAVAAELLHDPKERAEHLMLVDLGRNDVGRVAEYGTVEVTA
                     LLRVEKYSHVLHLVSDVMGKVRKGVSALQVLRATFPTGTLTGAPKVRAMQIIDDLETV
                     RRGPYAGALGYVAAGGARMELAVTIRSCVIADGVAAVQSGAGIVADSVPELEWIETEN
                     KARAIMSAIGRIPARKPAPTLPRRS"
BASE COUNT          533 a         1242 c         1223 g          557 t
ORIGIN      
        1 gaggcctttc gcgtgcgcgg acaccctgtc gccacgggcg agttcggcgc gatgatgagc
       61 gtcgagctgg tgaacgacgg gccggtgacg ctggtgctcg agcggtgatt ccgaaggtcg
      121 tcctggcgtc ggcttctccc cggcgccgcg agctgctcac gctggtcggc atcgagcacg
      181 ccgtcgtgcc ctcgcatctc gacgagacga tgcatcccgg cgagcagccg ggtccgcatg
      241 cccggcggct cgcggtcgag aaggcgacag ccatcgccgc aagccatcgc gacgccgtcg
      301 tcatcggggc ggatacggtg gtggtgctcg acggcaaggt gctgggaaag cccgccacgc
      361 ccgaagtcgc gcgggaaatg ctgcgcgcgc tcaacggacg gactcatacg gtgatcacgg
      421 cggtcgcggt ggcgtacgcg ggacgaacca gcgagggagc cgaagaagtc tccgtgacct
      481 tccgccagct ctctgacgac gagatcgacg cctacgtcgc cacgcgcgag gggatggaca
      541 aggccggcgc gtacggcatt cagggattcg gcgcgacgat cgtcgagcgc atagacggcg
      601 atttcttcgc cgtgatgggg ttgccgctcg tccgcctgat ctcgctcctg cgggacgtcg
      661 gtctgcgcta tcgcttcggt aacgtggaga gcgcggccgc cgagtagcga gccttccaga
      721 cgtccacctt ctcgaggacg tcccgcggcg tgatgcgcgc catccggccg gcccggtgct
      781 tggccgaggc gtcgtagctc tcaccgggat cgccgaacgc gtccacgatc aggtcgtgaa
      841 acttccggta cggcccgctg cgcttcgggt tggtgaatcc catgagcgag accacgggcc
      901 tgtccagcgc cacggccatg tgcagtggac ctgtatcggg cgcgagcacc agtgcggacg
      961 cgtccagaat gcacacgagg tttcgcagtc cgctgcccag cgcggagaag tgcgggtgct
     1021 tgctgcgctc gagaatcacc cgctcggccc gccgttcgcg ctccgatccc ccgccgacga
     1081 gcacgggctg caacccgtaa tcggcgtaca gcgcgtcgca cacttcggcc cagcgctcag
     1141 gggcccagtc cttcgctgcc ttgctggtgc ccaccacgac ggacgcgacg ggccggtcga
     1201 acctggcgga gaattcccgc tgccactcgc gctcgctctc ccacggtccg aggtgccatt
     1261 ccacgggctc gtgcggcacg cccatgtaag tgagaaactc gaagtactga tcctggacgt
     1321 gctgcggcgc gcgcggcggg atcgtgtggg tcgtgaacat cgtgttcatg tcgcgcgcgc
     1381 tccctgtcga atccgagctt caccggggcg cgcgtgaagg ccgtcacgac gcccgccttg
     1441 aagtacacct ggagattgag gaccacgtcg aactcgcgcg cgtccagcgc ggcgcggacg
     1501 tcgcggaagg cgcgcagtcc ggcgctccgg ttgaagacga tcgtctcgtc cacggagcgg
     1561 tggccacgca ccagcgaatt cggtccgggc tgcacgaccc aggtgatccg gctgcggggg
     1621 gaatgccgct tgatcgcgtt gataacgggc aggacgtgca ccgcgtctcc caccgcgctc
     1681 atcatgacta tgcacacgcg gtcgagggcg gccttcactc ggacagctcc tgcacccggt
     1741 ccaggatggc gaggatctct tcgtcggtta tggacagtcc gagccgcatg gactttctga
     1801 gcgaccggcg cagccgggtg atgttggcgt cacgcaccgt cggcgacccc gcggcgtgga
     1861 agcgaacgcg atccacgtcc aggatccagc cgatgagccc gccgtccggg cccggggcga
     1921 cgagcacgtt gcggatgttc aggtcggggt gatgcgcgcc cgcggcgacc agcgaagcca
     1981 ccgcgccggc ggcggcgttg agcacgacgt cgcgcgcgcc ttcgtcctcc gtcgcggcca
     2041 gccacgcgaa cagatcggcg ccgttcggaa tctcgcgcgt ggcgacgtcg aagcggcgga
     2101 gaatcgcgcc cgcgggatac gtcacgtacg cgacgacctc cggcgtgtgc actccggcgg
     2161 cgcggaggcg ctgcgacacc agcagctcgc gcaggccgcg cgtgggcggc aggaacaggt
     2221 cccgggtgac gtgcgcgggc gcgcctccgc gaaacgcgtg gcgaaccacg accgactcct
     2281 cgtggcccgg gagcggcgcg gcgaacacgt cgccccgtcc cttcatctgc cgccgctcgg
     2341 ggtggcgtcc ggcgtactcg tacagcgtgc cgtatcgcag cgcctcgcgg atcgacctct
     2401 cctcctcctc cagcgcggtc acccgcgcgc gtccgacgcg gaatcgcgcg tatcccggga
     2461 tctccaggcg catctcagct ccgccgtggc agcgtggggg ccggcttccg cgcggggatg
     2521 cgtccgatgg ccgacatgat cgcgcgcgcc ttgttctcgg tctcgatcca ctcgagctcg
     2581 ggcacggaat ccgccacgat cccggcgccc gactgcacgg ccgctacgcc gtccgcgatc
     2641 acgcacgacc ggatcgtcac cgcgagctcc atcctcgcgc caccggccgc gacgtagccg
     2701 agcgcgcccg cgtacgggcc gcgccgcacc gtctcgaggt cgtctatgat ctgcatggct
     2761 ctgactttcg gtgccccggt gagcgtgccc gtgggaaacg tagctcgcag gacctggaga
     2821 gcggagactc cctttcgcac cttgcccatt acgtcgctca cgagatggag cacgtgcgaa
     2881 tacttctcca cccgcagcag cgccgtcact tcgacggtcc cgtactcggc tacccgcccg
     2941 acgtcgttcc gcccgagatc caccagcatc agatgctcgg cgcgctcctt gggatcgtgc
     3001 agcagctcgg cggcgacggc gtcgtagccg ggcgtcgcgc cgggcctggg acgcggtctg
     3061 gtgcccgcga tgggccggac cgtggtcatt ccgtcctgca gccggaccag cagctccggc
     3121 gagctcccga cgatctccat ctcgtcgagc gtgagacagt acatgtacgg cgaagggttt
     3181 atcgcccgca gcgcgcgata caggtccaca gtgtcgaacg tcgcgggcag ctcgatgcgg
     3241 cgcgtgagca cgacctggaa tgcgtcgccc gcgaggatgt gctccttgac ccgcaccacc
     3301 gcgccttcga agctcgactt gtcgaggctg gacttgcctt ttacttcgcg atcgtcgccc
     3361 agctccagcg gcaggagggg cgacggcgcc cgcagccgcg cgcgcagctc gtccgcgtcg
     3421 gccacggcgg cggcgtatga ctcgcgcagc tccgcgtcgc tcgcgcccac cgggacctgg
     3481 acggtgatca cgatgttggc ggtgccgtgg agattgtcga tgacaacgag cgagcgcgtg
     3541 aagacgaaca tcgcg
//
LOCUS       DASTZR010000371         4452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_647066, whole genome shotgun sequence.
ACCESSION   DASTZR010000371 DASTZR010000000
VERSION     DASTZR010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647066"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="VFV20_08335"
     CDS             <1..1056
                     /locus_tag="VFV20_08335"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU5288397.1"
                     /translation="VVTLADPARAIAEAAIGTLCTACVVRVERRGTSDTFALGTLCPD
                     PEAGDDAPCTPDALFDLASLTKLVTSALVLSLVRERALDLATPFSELVPDFRGGRKDD
                     VTLRHVLTHTAGLRWWLPLWREVSSLEEAVWRAAQEPLAQDLGTFRYSDLGYIMLTQA
                     LATATGMPFADVARERVLGPLEAETCAFGPRPPGQCVATERDTEWRKKRLRGEVHDEN
                     AHAMGGVAGHAGLFGTAADVAKIARVYRDGAVIGNELARLARTEQVRGENVRRGLGLA
                     LRAPHDAMVGPHFSIDAYGHTGFTGTSLWIDPQLDLTVILLTNRVYFGRTNDEEMYRF
                     RIAVHEAVSRPFVRAAA"
     gene            1053..2156
                     /locus_tag="VFV20_08340"
     CDS             1053..2156
                     /locus_tag="VFV20_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011526054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEU5288398.1"
                     /translation="MIAVGIMSGTSADGADAAAIELDVPATRVRVLATASEPYAPSLR
                     DAVLALGEGQPIDARAVARTHAALGDHYARLAERIARGLPRVPDVVTVHGQTVAHLPA
                     DHVTLQIGDAARVAQRTGTPTVSDLRSADLALGGQGAPLVPFGDLVLFGSLAPVAVLN
                     IGGIANLTIVASDDPHAVVAFDTGPGNILIDGIAALEGRTHDEDGAGAKRGRVDTETL
                     SEFLAHPYFARRAPKSTGREDFGRAFAGRLAEIVARHGGSHDDALATATALTARTIAD
                     ALRRESGKSIRRLLVAGGGARNPTLVAMLRDALAPEVGTIEPTDVHGVPAAYREAIAF
                     AILGAYRLRGLPNTLPRATGAARAVSGGALHLP"
     gene            2224..3138
                     /locus_tag="VFV20_08345"
     CDS             2224..3138
                     /locus_tag="VFV20_08345"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEU5288399.1"
                     /translation="MRSGIVVGIDAGATKTRAFAVDRAGEVVGRGAGGGANLLSSSDP
                     QGSIGAALAEALGDRGADAVVLAAAGGDRKADRERGRELLARVAPAGTQLLVTHDAIA
                     ALYAGNPAGCGVVLIAGTGSIAYGRNEEGEEDRAGGWGYLIGDEGSAVWCGLEALRAV
                     AHAVDGRGMPTRLVDLLFQELGVGEFADVLPHLYGRSHPAPAIAAATRALALASADGD
                     AAANGILQRGATALARAATVVATTLGLVGGPVYLAGGAFETLQLLQRLVRLELLGALP
                     RATVELTHEEPAMGAARIAMKLAWGGPA"
     gene            3135..3962
                     /gene="murQ"
                     /locus_tag="VFV20_08350"
     CDS             3135..3962
                     /gene="murQ"
                     /locus_tag="VFV20_08350"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEU5288400.1"
                     /translation="MSEERRTEARNPRAAALASFGTREILELMNDEDALVPAAVRAAI
                     PAIASAVDAIVTSFADGGRLRYFGAGTSGRLGVLDASEAPPTFGVDGDLVRGIMAGGD
                     GALRTSIEGAEDDAGAGARDARRWVRSSDVAVGISASGRARYVVGAIDTAREIGARTV
                     ALTCDATSPLASGADIAIALDVGPEVLAGSSRLKAGTATKLVLNMLTTASMIRSGRTR
                     GDLMIDLRATNTKLRERAIRIVRDETGADEAAARASLETSGWNVRAALDALEKRAPR"
     gene            3962..>4452
                     /locus_tag="VFV20_08355"
     CDS             3962..>4452
                     /locus_tag="VFV20_08355"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEU5288401.1"
                     /translation="MRLIVNADDLGYTPGVTRGILQAHREGIVTSATLMANAADSEGA
                     GALARRTPDLDVGVHVVFTYGRALTAPERIRSLVDERGAFPRVTDLLRSGQPLAEHAL
                     IEARAQYERARELIGREPTHLDTHHWVHDMRALEDAMLALAKETGMALRAHDGRQRAR
                     FRD"
BASE COUNT          581 a         1625 c         1669 g          577 t
ORIGIN      
        1 gtcgtgacgc tcgcggatcc ggcgcgcgcc atcgccgagg cggcgatcgg aacgctctgc
       61 acggcgtgcg tcgtgcgggt ggagcggcgc ggcacgtcgg acacgttcgc gctgggcacg
      121 ctctgccccg acccggaagc cggtgacgac gcgccctgca cgcccgacgc gctgttcgat
      181 ctcgcctcgc tgaccaagct cgtcacgagc gcgctcgtcc tgtccctcgt gcgcgagcgc
      241 gcgctcgatc tggcgacccc gttcagcgag ctcgtgcccg acttccgcgg cgggcgcaag
      301 gacgacgtca cgctgcggca cgtgctcacg cacaccgcgg ggctgcggtg gtggctcccg
      361 ctctggcgcg aggtgtcgtc gctcgaggag gcggtatggc gcgcggcgca ggagccgctc
      421 gcgcaggacc tcggcacgtt ccgctacagc gacctcggct acatcatgct cacgcaggcg
      481 ctcgccaccg ccacgggcat gccgttcgcc gacgttgcgc gcgagcgcgt gctcggtccg
      541 ctcgaggccg agacctgcgc gttcgggccg cgtccgccgg ggcagtgcgt cgcgaccgag
      601 cgcgacaccg agtggcggaa gaaacggcta cgcggcgagg tccacgacga gaacgcgcac
      661 gcgatgggcg gcgtcgcggg acacgccggc ctcttcggga cggctgccga cgtggcgaag
      721 atcgcccgcg tgtatcggga cggcgccgtc atcgggaacg agcttgcgcg gctcgcccgg
      781 acggagcagg tgcgcgggga gaacgtgcgg cgcggcctcg gcctggcgct ccgcgcaccc
      841 cacgacgcga tggtcggccc gcacttcagc atcgacgcct acgggcacac cgggttcacc
      901 ggcacctcgc tctggatcga cccgcagctc gacctcaccg tcatcctctt gacgaatcgc
      961 gtgtacttcg ggcgcacgaa tgacgaggag atgtatcgct tccgcatcgc cgtgcacgag
     1021 gcggtctcgc gtcccttcgt gagggccgcg gcgtgatcgc ggtcggcatc atgtcgggta
     1081 cgtcggccga cggcgcggac gccgcggcga tcgagctcga cgtcccggcg acgcgggtcc
     1141 gcgtgctcgc gaccgcgagc gagccctacg cgccgtcgct gcgggacgcg gtgctcgcgc
     1201 tcggcgaggg gcagccgatc gacgcacgcg ccgtcgcccg aacgcacgcg gcactcggcg
     1261 atcactacgc gcgccttgcc gagcggatcg cgcgaggtct tccccgcgtg cccgacgtcg
     1321 tgacggtgca cgggcagacc gtggcgcatc tccccgccga tcacgtgacg ctccagatcg
     1381 gcgacgcggc gcgcgtcgcg cagcggaccg gcactccgac cgtgagcgac ctccgcagcg
     1441 ccgacctcgc gctcggcggt cagggcgcgc cgctcgtgcc gttcggagac ctcgtgctct
     1501 ttgggtcgct cgcaccggtc gcggtcctca acatcggcgg catcgcgaat ctcacgatcg
     1561 tcgcgagcga cgatccgcat gcggtcgtcg cgttcgacac cggacccggc aacatcctca
     1621 tcgacggcat cgccgcgctc gagggccgca cccacgacga ggacggcgcg ggcgcgaaac
     1681 gcggccgtgt cgacacggag acgctctccg agttcctcgc gcatccctac ttcgcgcgcc
     1741 gcgcaccgaa gagcactggg cgcgaggact tcggccgcgc gttcgcgggg cgcctcgccg
     1801 agatcgtcgc ccgccacggt gggtcgcacg acgatgccct cgccacagcc accgcgctca
     1861 ccgcgcggac catcgccgat gcgctgcgtc gagagtcggg caagtcgatc cgtcgcctgc
     1921 tggtggcggg cggcggcgcg cgcaatccca cgctcgtcgc gatgctccgg gacgccctcg
     1981 cgcccgaggt cggcaccatc gagccgaccg acgttcacgg ggtgcccgcc gcgtaccgcg
     2041 aagcgatcgc gttcgcgatc ctgggcgcct accggcttcg cggcctgccg aacacgctgc
     2101 cacgcgcgac gggcgcggct cgtgccgtct ccggcggcgc gctccatcta ccgtaggtgg
     2161 tcatgcgctc cctcggcgtg cctgaccggc gtgctgctcc gccttcggct gcgcggtagc
     2221 ggcgtgagat ccgggatcgt cgtcgggatc gacgccggcg ccacgaagac gcgcgcgttc
     2281 gcggtcgacc gggccggcga ggtggtcggt cgcggggcgg gcggcggtgc gaatctgctc
     2341 tcctcctccg atccgcaggg atcgatcggg gcggcactgg ccgaggcgct cggcgaccgt
     2401 ggcgcggacg ccgtcgtgct cgccgcggcc ggcggagatc gcaaggccga tcgcgagcgc
     2461 ggccgcgagc tgctcgcgcg cgtcgcgccg gcgggcacgc agctgctcgt gacgcacgac
     2521 gcgatcgcgg cgctgtacgc ggggaatccc gcgggctgcg gggtggtgct catcgccggg
     2581 acgggctcga tcgcatacgg acggaacgag gagggcgagg aggatcgcgc cggtggctgg
     2641 ggctacctca tcggcgacga gggatccgcg gtgtggtgcg gcctcgaggc gctacgcgcg
     2701 gtcgcgcatg ccgtggacgg tcgcgggatg ccgacccgcc tggtggacct gctcttccag
     2761 gagctcggcg tgggcgagtt cgcggacgtc ctgccgcatc tgtacggacg gtcgcatccg
     2821 gcgccggcga tcgccgcggc gacgcgggca ctggcgctcg catcggcgga cggcgacgcc
     2881 gccgcgaacg gcatcctgca gcgcggcgcc accgcgctgg cgcgcgccgc cacggtcgtc
     2941 gcgacgacgc tcggactcgt cggcggaccc gtgtacctcg ccggcggtgc gttcgagacc
     3001 ctgcagctgc tgcagcggct cgtgcgtctc gagctgctcg gcgcgctccc gcgcgcgacg
     3061 gtcgagctga cccacgagga gccagccatg ggcgccgcgc ggatcgccat gaagctcgcg
     3121 tggggtggtc ccgcgtgagc gaggagcggc gcacagaggc gcggaacccg cgcgccgccg
     3181 cgctcgcgtc gttcggcacc cgcgagatcc tcgagctcat gaacgatgag gacgcgctcg
     3241 tacccgcggc ggtgcgcgcg gcgatcccgg cgatcgcgag cgcggtggac gcgatcgtca
     3301 cgtcgttcgc ggacggggga cgtctgcgct acttcggcgc ggggacgagc ggacggctcg
     3361 gcgtcctcga cgcgagcgaa gcgccgccga ccttcggcgt ggacggcgat ctcgtgcgcg
     3421 ggatcatggc gggcggcgac ggcgcgctgc gcacatcgat cgagggcgcc gaggacgacg
     3481 ccggcgcggg cgcccgcgac gcccgccggt gggtgcggtc gagcgacgtc gccgtcggga
     3541 tctcggcgag cggccgggcg cgctacgtcg tcggcgcgat cgacacggcg cgggagatcg
     3601 gagcgcgcac ggtcgcgctc acctgcgacg cgacgtcgcc actcgcgtcc ggtgcggaca
     3661 tcgcgatcgc gctcgacgtg gggcccgagg tgctcgcggg atcgagccgg ctcaaggcgg
     3721 gcacggccac gaagctcgtc ctgaacatgc tcaccacggc atcgatgatc cgatcggggc
     3781 gcaccagggg cgatctcatg atcgacctcc gagcgacgaa cacgaagctg cgcgagcgcg
     3841 ccatccgcat cgtgcgcgac gagaccggcg cggacgaagc cgccgcgcgg gcgtcgctcg
     3901 agacgagcgg ctggaacgtg cgcgcggccc tcgacgcgct ggagaagcga gcgccgcgct
     3961 gatgcgcctc atcgtcaacg cggacgatct cggctacacg cccggcgtga cacgcggcat
     4021 cctccaagcg caccgtgagg gcatcgtcac gtcggcgacg ctcatggcga acgcggccga
     4081 cagcgagggc gcgggcgccc tcgcccgacg cacgccggat ctggacgtcg gcgtccacgt
     4141 cgtcttcacc tacgggcggg ccctcacggc gccggagcgc atccgctcgc tcgtggacga
     4201 gcgcggcgcg ttccctcgcg tcaccgacct cctgcgcagc gggcagccgc tcgccgagca
     4261 cgcgctgatc gaggcgcgcg cgcagtacga gcgcgcgcgg gagctcatcg ggcgcgagcc
     4321 cacgcatctc gacacacacc actgggtgca cgacatgcgc gcgctcgagg acgcgatgct
     4381 cgcgctcgcg aaggagacgg ggatggcgtt gcgggcgcac gacggccgcc agcgcgcgcg
     4441 gttccgcgat ga
//
LOCUS       DASTZR010000372         3536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_852100, whole genome shotgun sequence.
ACCESSION   DASTZR010000372 DASTZR010000000
VERSION     DASTZR010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852100"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..370)
                     /locus_tag="VFV20_08360"
                     /pseudo
     CDS             complement(1..370)
                     /locus_tag="VFV20_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404453.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
     gene            complement(424..1077)
                     /locus_tag="VFV20_08365"
     CDS             complement(424..1077)
                     /locus_tag="VFV20_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEU5288402.1"
                     /translation="MRRLVVGTFLTLDGVMQAPGGPEEDREGGFERGGWSVNYWDELM
                     GKTIVEWTVRAGALLLGRKTYQIFAAHWPKVSDDDPVARTLNSVPKYVASRTLKEVAW
                     RNSTLLQGDVAEAVARLKRSEGEEIQVHGSGDLIQTLLGHDLVDELRLWFFPVSLGHG
                     KRLFAGGTLPRTFTLTDTKTSTTGVAIHTYQAAGGIRYGSFAVDEHGRADALWRDTP"
     gene            complement(1284..1682)
                     /gene="rplL"
                     /locus_tag="VFV20_08370"
     CDS             complement(1284..1682)
                     /gene="rplL"
                     /locus_tag="VFV20_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEU5288403.1"
                     /translation="MAVETNPKFEKLAGELESWSILEIAGFGKYLQERWGVSAAPVAV
                     SAAPGAGGGAAAAAEAEEKTEFTVVLKEAGASKINVIKTVRELTGLGLKEAKDLVDGA
                     PKPVKENIGKDEAESIKKKLEDAGASVELK"
     gene            complement(1699..2247)
                     /gene="rplJ"
                     /locus_tag="VFV20_08375"
     CDS             complement(1699..2247)
                     /gene="rplJ"
                     /locus_tag="VFV20_08375"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEU5288404.1"
                     /translation="MAKIKTGPKTKRKGGGIPAKSRAVDALAAQLSASTAVIVTEYRG
                     LKVTELQELRKRLRPKGVEYHVVKNSLLSRAASKTQRESLGKLLAGPTAVALTKGDEV
                     ELAKGIVDELRGLKALKIVGGMAGTQALTAEDVTSLARLPGRAYLQGQIVGSLQAPLG
                     QLTGLLQAPFQNLIHVLTARSA"
     gene            complement(2400..3122)
                     /gene="rplA"
                     /locus_tag="VFV20_08380"
     CDS             complement(2400..3122)
                     /gene="rplA"
                     /locus_tag="VFV20_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEU5288405.1"
                     /translation="MPEAKHGKRYDELAKLIERGREYSPDEAVRLVKETSKAKFDTTV
                     EVHLRMGVDPKQADQMVRGSAVLPNGTGKTVRVIVFAQGDRARDAQAAGADAVGAEDL
                     AKRIEGGWLDFDVAIATPDMMGQVGRLGRVLGPRGLMPNPKSGTVTLDVAKAVRDSKG
                     GRVEFRVDKTGVIHTVVGKSSFTEQALLGNLATIMDAVNRARPTGVKGEYVETVFLTS
                     THGPSVKIARGDALALAGRSAA"
     gene            complement(3201..>3536)
                     /gene="rplK"
                     /locus_tag="VFV20_08385"
     CDS             complement(3201..>3536)
                     /gene="rplK"
                     /locus_tag="VFV20_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEU5288406.1"
                     /translation="HGINMGQFIKEYNERTAPLAGTVVPAVVTIFEDRSYTFVTKSPP
                     AADLIRKEAGIEKGSGKPNKEKVGKITKAAVKKIAETKMNDLNATSLESAMKMVEGTA
                     RSMGVEIAG"
BASE COUNT          491 a         1220 c         1165 g          660 t
ORIGIN      
        1 cgaggtcgat cgcctcggcg ccggtgtgtt cgacattcgc gcgggtcgtg tcgaagtgcg
       61 tgttgttcgg gacgaccttg cccggcccac cgagcacgct gaagatgatc ttctcggcgg
      121 cgcgtcactg gtgcgtgggg atgatgtgcg cgaacgggaa gagctcgcga acggcctcgc
      181 ggaacacgaa atatgagggc gaaccggcgt aggactcgtc gccgtgctgg atcgcggccc
      241 actggtcgcg ggacatcgcg ccggtcccgg aatcggtgag gaggtcgatg agcacgtcgt
      301 cggcgtgcag cgcgaagagg ttgtaggcgg cctcgcggat gcgctcgcgc cgctgctcgg
      361 gggtcgtcat gcggagcggt tccaccgagt ggatccggaa cggctcgatg atcgtgcgga
      421 aggtcacggc gtatcgcgcc acagcgcgtc ggcacgtccg tgttcgtcca ccgcgaacga
      481 tccgtagcgg atgccgcccg ccgcctggta ggtgtggatc gcgacgcccg tcgttgacgt
      541 cttcgtgtcg gtgagtgtga acgtccttgg gagcgtgccg ccggcgaaca gccgcttccc
      601 gtgtcccaga gatacaggaa agaaccagag gcgcagctcg tcgacgaggt cgtgaccgag
      661 cagggtctgg atgagatcgc cgctgccgtg gacctggatc tcctcgccct ctgaccgctt
      721 caaccgcgcg acggcctcgg cgacgtcacc ctgcagcagc gtggagttgc gccaggccac
      781 ctccttgagc gtccgcgagg cgacgtactt cgggacgctg ttcagggtcc tcgcgacggg
      841 atcgtcgtcg ctcaccttcg gccagtgcgc cgcgaagatc tgatacgtct tgcgcccgag
      901 cagcagtgcc cccgcccgca cggtccactc gacgatcgtc ttgcccatga gctcgtccca
      961 gtagttgacc gaccagcccc cgcgctcgaa tccgccttca cggtcctcct cgggaccgcc
     1021 gggagcctgc atcacaccgt cgagggtgag gaacgttccg acgaccagcc tgcgcatctc
     1081 ggcccgtcct ttcggcccgg aaaaccaaac cggagccctc tcgggccccg gtccggcacc
     1141 tttcgcgagc ccaccgacct gaaccggcgg agtcgctgcg agaacgatat cgggggtcgt
     1201 ttgcgctgcc atttctggca gcagaacaga cgaccgaacg gctgaacgat gtggccgcag
     1261 gggccgaaaa ccggcccttg cggttatttc agctcgacgc tcgcgcccgc gtcctcgagc
     1321 ttcttcttga tcgactcggc ctcgtcctta ccgatgttct ccttcaccgg cttgggcgcg
     1381 ccgtcgacga ggtccttcgc ctccttgagg ccaaggcccg tgagctcgcg gaccgtcttg
     1441 atgacgttga tcttcgacgc gccggcctcc ttgaggacga cggtgaactc ggtcttctcc
     1501 tccgcctcgg ccgcggcggc ggcaccgcca ccggcgcccg gagccgcgct gaccgcgacc
     1561 ggcgccgcgg agacgcccca gcgctcctgg aggtacttgc cgaatcccgc gatctccagg
     1621 atcgaccagg actcgagctc gcccgcgagc ttctcgaact tcggattggt ctcgactgcc
     1681 atctctatct ccttgtagtt aggccgaccg cgcggtcagc acatggatga ggttctggaa
     1741 cggtgcctgg agtagtccgg tgagctgacc gagcggagcc tggaggctgc cgacgatctg
     1801 gccctgcagg tacgcgcggc ccggcagacg cgcgagcgag gtgacgtcct cggccgtgag
     1861 cgcctgcgtg ccggccatcc cgccgacgat cttcagcgcc ttcagcccgc ggagctcgtc
     1921 gacgatgccc ttcgcgagct cgacctcgtc gcccttcgtg agcgcgaccg cggtcggacc
     1981 cgcgaggagc ttgcccagcg actcgcgctg ggtcttcgac gcggcacgtg agaggaggct
     2041 gttcttcacg acgtggtact ccacgccctt cggacgcagc cgcttgcgca gctcctggag
     2101 ctcggtgacc ttgagccccc ggtattcggt gacgatgacc gcggtcgagg cggagagctg
     2161 cgcggcgagc gcgtccacgg cgcggctctt cgccgggatg ccgccgccct tgcgcttcgt
     2221 cttcggcccg gtcttgatct ttgccatctg tcctccgttc tacaaacaaa accctcgttc
     2281 ccgagtgcgg agaacgaggg cacgcgcggc catgccgcgc tggctcttgc ctccgcagga
     2341 cttatctccg ctttcgcgga gtgccggctt ctttggctat tcgcgagtgc tgtggctaat
     2401 tatgcggccg agcggcccgc gagcgccagg gcgtcgccgc gagcgatctt cacgctcggc
     2461 ccgtgggtgc tcgtgaggaa gacggtctcg acgtactcgc ccttcacgcc ggtcgggcgc
     2521 gcccggttca cggcgtccat gatcgtcgcg aggtttccga ggagtgcctg ctcggtgaac
     2581 gaggacttgc cgaccaccgt gtggatcacg ccggtcttgt cgacgcggaa ctcgacgcgg
     2641 ccgcccttcg agtcgcgcac cgccttggcg acgtccagcg tcacggtgcc ggacttgggg
     2701 ttcggcatga ggccgcgcgg tccgagcacg cgtccgaggc gaccgacctg gcccatcatg
     2761 tccggcgtcg cgatcgcgac gtcgaagtcc agccagccgc cctcgatgcg cttggcgaga
     2821 tcctccgcgc ccacggcgtc ggcaccggcg gcctgcgcgt cccgcgcgcg gtcgccctgc
     2881 gcgaacacga tcacgcggac ggtctttccg gtgccgttgg gaagcacggc cgagccgcgc
     2941 accatctggt cggcctgctt cggatcgaca cccatgcgga ggtgcacctc gacggtggtg
     3001 tcgaacttgg ccttgctggt ctctttcacg aggcggaccg cctcgtcggg tgaatactcg
     3061 cgaccgcgtt cgatcagctt cgcgagctcg tcgtagcgct tgccgtgctt cgcctctggc
     3121 atgtcaacgc tcctctcggc cgttcggcct cccgtctcac gcgcggtgca cgctcgccgt
     3181 cgcggcgaga taccgagatg ttagccggcg atctccacgc ccatgctccg cgccgtgccc
     3241 tcgaccatct tcatggccga ctcgaggctc gtcgcgttca ggtcgttcat cttggtctcg
     3301 gcgatcttct tcaccgcagc tttcgtgatc ttcccgacct tctccttgtt cggcttgccg
     3361 gaacccttct cgatgccggc ctccttgcgg atgaggtcgg ccgcgggcgg ggacttcgtg
     3421 acgaaggtgt acgagcgatc ctcgaagatc gtcaccaccg cggggacgac cgtgcccgcg
     3481 agcggcgcgg tgcgctcgtt gtactccttg atgaactggc ccatgttgat gccgtg
//
LOCUS       DASTZR010000373         2104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_255890, whole genome shotgun sequence.
ACCESSION   DASTZR010000373 DASTZR010000000
VERSION     DASTZR010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255890"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(333..>2104)
                     /locus_tag="VFV20_08390"
     CDS             complement(333..>2104)
                     /locus_tag="VFV20_08390"
                     /inference="COORDINATES: protein motif:HMM:NF018757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEU5288407.1"
                     /translation="ARQELAGLPLAGMVLVSDGADTTDASLSEALLALKASSVPVFTV
                     GVGRDRLARDVQIDRVSTPRTALKGTSLVIDAVVTQTGYAGETVSLDVEDEGRIVGSQ
                     DVKLPIDGEPAAVRVRFTAGDAGARVFKFRITPRPGELVTQNNTREALIDIADRREKI
                     LYYEGEPRPEMKFINRAVADDKNLGVVTLQRTADTKYLRLGVDNPEQLLAGFPKTREE
                     LFGYRGLILGSIEAGAFSGDQLRMIGEFVERRGGGVIMLGGSRSFAEGGYAGTPVADM
                     LPVILDRPARSVENLPVARLHVKPTRAGEAHGVTQIAPTEAASNARWPEMPILTSVNT
                     IRDVKPGATVLLNGTDANRRDQVVLAWQRYGRGKTLAFPVQDSWHWQMDVKMSLEDQT
                     HENFWRQLLRWLVDGVPEAVDVHTTTDRVEPGETVTLVADVVDPAFMEVNDARVVATV
                     TGPKGAPIDVPMQWTGERNGEYRATLPAPDLGMYVARVEATRAGKTLGTGTTQVRAVP
                     SDAEYFDATMHAARLQRIADETGGRFYTPDTVSGMPEDLRYTGRGVTTVEERELWHMP
                     IVLMALIALIGAEWGYRRAVGLA"
BASE COUNT          296 a          756 c          705 g          347 t
ORIGIN      
        1 tgatccgaca gatagaacac gcgatcggga gcgacgccga gacgccccgt cgcgccgtcg
       61 acgagcgtcg aggcccagcg ctgaaagagc tccgcgtgct cgggatcgcc ggcgaggccg
      121 acgacgacca tcaggtgtac gttctgcgcc gcggccggcg ccgcgagcgc cagcattatg
      181 aacaggacaa tcacgctgct gcgaggcacg tggttagttc cactctcggt ctgcatttat
      241 cactgcgcgt ggggccccac ccccacgcgc tctcgctcgc gggcgccgct ccgcggcgct
      301 tccctccggc cgctcgctcg ggccgcaggc gctcatgcga gtcctaccgc ccggcgatag
      361 ccccactcgg cgccaatcaa cgcgatcaag gccatcagca cgatcggcat gtgccagagc
      421 tcgcgctctt caaccgtcgt gacgccgcgt cccgtgtagc gcaggtcctc gggcatgccg
      481 ctgacagtgt cgggcgtata gaagcgtccg ccggtctcgt ccgcgatccg ctgcagccgc
      541 gccgcgtgca tcgtggcgtc gaagtattcg gcgtcgctcg ggacggcgcg cacttgcgtc
      601 gtcccggtgc cgagcgtctt gccggcgcgg gtcgcttcga cccgggcgac gtacatgccc
      661 aggtcgggcg ccggtaacgt ggcgcggtat tcgccattgc gctcgccggt ccactgcatc
      721 ggcacgtcga tcggcgctcc tttcggtccc gtgaccgtgg ccacgacgcg cgcgtcgttc
      781 acttccatga aggccgggtc cacgacgtcg gccacgagcg tgaccgtctc gcccggttcg
      841 acgcggtcgg tcgtcgtgtg cacgtcgacc gcctcgggaa cgccatcgac cagccatcgc
      901 agcagctgac gccagaagtt ttcgtgcgtc tggtcctcga ggctcatctt cacgtccatc
      961 tgccagtgcc acgagtcctg cacggggaag gcgagcgtct tcccgcggcc gtaacgctgc
     1021 caggccagca cgacctgatc gcgccggttc gcgtcggtgc cattcagcag cacggtcgcg
     1081 cccggcttga cgtcgcgaat ggtgttcacg ctcgtgagga tcggcatctc gggccagcgc
     1141 gcgttcgacg ccgcttccgt cggcgcaatc tgcgtgacgc cgtgcgcttc tccggcgcgc
     1201 gtgggcttca cgtgcaggcg cgcgacgggc aggttctcca ccgagcgggc gggccgatcg
     1261 aggatcaccg gcagcatgtc ggccaccggc gttccggcat agccgccttc ggcgaacgag
     1321 cgcgacccgc cgagcatgat gacgccgccg ccgcgccgct cgacgaactc gccgatcatg
     1381 cgcagctgat cgccggagaa cgcgcccgct tcgatgctgc cgaggatgag cccgcgatag
     1441 ccgaagagct cctcgcgcgt cttcgggaaa ccggccagca gctgctcggg attgtcgacg
     1501 ccgagccgca ggtatttcgt gtcggcggtg cgctgtagcg tgacgacgcc gaggttcttg
     1561 tcgtcggcaa ccgcgcggtt gatgaacttc atctcggggc gcggctcgcc ttcgtaatag
     1621 agaatctttt cgcgccggtc cgcgatgtcg atcagcgcct cccgcgtgtt gttctgggtg
     1681 acgagctcgc cgggacgcgg ggtgatccgg aacttgaaca cgcgcgcacc ggcgtcgccc
     1741 gctgtgaacc gtacgcggac ggccgccggc tcgccgtcga tcggcagctt cacgtcctgc
     1801 gacccgacga tccgtccttc gtcctcgacg tcgagcgaca cggtctcgcc ggcgtagccg
     1861 gtctgcgtga cgacggcgtc gatgacgagc gacgtcccct tgagcgccgt ccgcggcgtg
     1921 gagacgcgat cgatctggac gtcgcgcgcg agccgatcgc gcccgacgcc gacggtgaag
     1981 accgggaccg acgacgcctt gagcgcgagc agcgcttcgc tgagcgacgc gtccgtcgtg
     2041 tccgcaccgt cgctcaccag caccatcccc gcgagcggca gccccgcgag ctcctggcgc
     2101 gcgc
//
LOCUS       DASTZR010000374         2038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_961110, whole genome shotgun sequence.
ACCESSION   DASTZR010000374 DASTZR010000000
VERSION     DASTZR010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961110"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VFV20_08395"
     CDS             <1..660
                     /locus_tag="VFV20_08395"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU5288408.1"
                     /translation="VALALVCAVRGHRLRLVMPDRVSLERKRIARAFGAELVLTDPLE
                     GTDGAILEARRLAAGDARYAYVDQYSNPANPLAHELTTGPEIWERSDGRVTHFVAGVG
                     TSGTVVGVSRSLRPKGVRIVAVQPDDALHGIEGWKHMPSALRPAIWDESVADVHMTQG
                     TDEALDMTRRLAREEGIFTGPSGGAAVAAALRVAREQAGRIVVLIADGGERYLSTPLW
                     E"
     gene            664..942
                     /locus_tag="VFV20_08400"
     CDS             664..942
                     /locus_tag="VFV20_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018540080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEU5288409.1"
                     /translation="MSVTVRLPGALRDATGGQTKLEASGRTLADVIADIERRHPGFRA
                     RVVDDAGRLRTYVNVYIGEDDARAKGGTEAPVPDGSEVMVIPAMAGGA"
     gene            972..1364
                     /locus_tag="VFV20_08405"
     CDS             972..1364
                     /locus_tag="VFV20_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017624979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEU5288410.1"
                     /translation="MRDEIVAHARASAPNEACGLVAAKGGTPTRLIRCANVHEMPTTR
                     YRIEPREQLRAFRDMDDRGEDLLAIYHSHPVSQPYPSPTDRAEAHYPEAFYVLVSLRA
                     EEPEVEAYRIKPDGAVTRVPLVSSDPPR"
     gene            complement(1345..>2038)
                     /locus_tag="VFV20_08410"
     CDS             complement(1345..>2038)
                     /locus_tag="VFV20_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288411.1"
                     /translation="PLTLAIACAIVLILVARVVLGIGINIRAILPPVMAVIVFALVLV
                     LAVLGLGDQANRMRYLAYALAPQLPAGLSTAIVSDFAAAPASGTRMEMLGYELTLPAG
                     WKNTTTGATGEAGRFESADATLVVARAGGDALSTADSYADRVAAPLKNGLTNAWQQRS
                     VTRINGVIVVDDRYGGVYEGRTLLWRQFTAVPGTQGLALVYRVVEPANAQSSLDEAAA
                     IAATWRITAEGR"
BASE COUNT          299 a          714 c          750 g          275 t
ORIGIN      
        1 gtcgcgctcg cgctcgtgtg cgccgtgcgc ggtcaccggc tgaggctcgt gatgcccgac
       61 cgcgtctcgc tcgagcgcaa gcgcatcgcg agggccttcg gcgccgagct cgtgctcacc
      121 gatccgctcg aggggaccga cggggcgatc ctcgaggcgc gacgtctcgc ggctggcgat
      181 gcgcgctacg cctacgtaga ccagtactcc aatccggcga acccgctcgc gcacgagctg
      241 accaccggac ccgagatctg ggagcggtcc gacggccgcg tcacgcactt cgtcgcgggc
      301 gtgggcacgt ccggcaccgt cgtcggcgtg tcgcgatcgc tccggccgaa gggtgtgcgc
      361 atcgtcgcgg tgcagcccga cgacgcgctg catggcatcg agggatggaa gcacatgccg
      421 tcggcactgc ggccggcgat ctgggacgag tcggtggccg atgtgcacat gacgcagggg
      481 accgacgagg cgctcgacat gacgcgccgc ctggcacggg aagagggcat cttcaccggg
      541 ccatcgggcg gggcggcggt ggcggccgcg ctgcgcgtcg cgcgggaaca agcgggccgt
      601 atcgtcgtgt tgatcgctga tggtggcgag cgctacctca gcacaccgtt gtgggagtga
      661 tacatgtcag tgaccgtgcg ccttcccggc gcgctgcgtg acgcgaccgg cggccagacg
      721 aagctcgagg cgtcgggtcg cacgctcgcg gacgtcatcg cggatatcga gcgccggcac
      781 ccgggcttcc gcgcgcgggt ggtcgacgac gccggccggc tgcgcacgta cgtgaacgtg
      841 tacatcggcg aggacgacgc gcgtgccaag ggcggcaccg aggcgccggt ccccgacggc
      901 agcgaggtca tggtgatccc ggcgatggcg ggcggcgcat aacggccgag accctcgccc
      961 tgccgacgac gctgcgtgac gagatcgtcg cgcacgcgcg ggcgtcggcg ccgaacgagg
     1021 cctgcggtct cgtggcggcg aagggcggca cgccgacgcg tctcatccgc tgcgccaacg
     1081 tgcacgagat gccgaccacg cggtatcgca tcgagccgcg cgagcagctg cgtgccttcc
     1141 gcgacatgga cgaccgtggt gaggaccttc tcgcgatcta ccacagccac ccggtgagcc
     1201 agccctatcc gtcgccgacc gatcgcgccg aggcgcacta cccggaggcg ttctacgtgc
     1261 tcgtgtcgct gcgcgccgag gagccggagg tcgaggcgta ccgcatcaag ccggatggcg
     1321 cggtgacgcg cgtgccgctg gtgtctagcg accctccgcg gtgatgcgcc aggtcgcggc
     1381 gatcgccgcg gcctcgtcga gcgacgactg cgcgttcgcc ggctcgacca cgcgatagac
     1441 cagggcgagc ccctgcgtgc cgggcaccgc ggtgaactgc cgccacagca gcgtgcggcc
     1501 ctcgtacaca ccgccgtagc ggtcgtcgac cacgatcacg ccgttgatgc gcgtcacgct
     1561 gcgctgctgc cacgcgttcg tcagcccgtt cttcagcggg gccgccacgc gatccgcgta
     1621 gctgtccgcc gtggacagcg cgtcaccgcc cgcgcgcgcg acgacgaggg tcgcgtccgc
     1681 cgactcgaag cgtccggcct caccggtcgc gccggtggtc gtgttcttcc agcccgccgg
     1741 cagcgtgagc tcgtagccga gcatctccat gcgagtgccg gacgcgggcg ccgcggcgaa
     1801 gtcggagacg atcgccgtgg acagtcccgc cggcagctgc ggcgcgagcg cgtacgcgag
     1861 gtagcgcatg cgattcgcct gatcgcccag tccgagcacc gcgaggacca acacgagcgc
     1921 gaacacgatc acggccatca ccggcggcag gatcgcgcgg atgttgatgc cgatgccgag
     1981 gacgacgcgc gcgacgagga tcaggacgat ggcgcacgcg atcgccaggg tcaggggc
//
LOCUS       DASTZR010000375         5312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_882574, whole genome shotgun sequence.
ACCESSION   DASTZR010000375 DASTZR010000000
VERSION     DASTZR010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882574"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /gene="rsmH"
                     /locus_tag="VFV20_08415"
     CDS             <1..522
                     /gene="rsmH"
                     /locus_tag="VFV20_08415"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU5288412.1"
                     /translation="IVNSWGERELRRVFSDFGEEPEAGRIAAAIVRRRSREPFLTADD
                     LGRFVFGVKRQRSRAIDPATRVFQALRIAVNDELANLERGLVAALSLLRPGGRLAVIS
                     FHSLEDRAVKGFMARESRDCICPPHLPTCVCGHRAGLRVVTRRPLRADSAEVDRNPRA
                     RSAALRVAEKIAS"
     gene            519..905
                     /locus_tag="VFV20_08420"
     CDS             519..905
                     /locus_tag="VFV20_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288413.1"
                     /translation="MIASQAVRAAMPRRVVRPRPRHAQRLARASARATFEVRTFAGVS
                     AAIAIAFFLSMLYLAQATTVSTLGYQAQHLQQVRDELRRQNALLEVENARLDSPARIE
                     TEAAKLGLVRAAQIPVVQLEPITAKR"
     gene            962..2644
                     /locus_tag="VFV20_08425"
     CDS             962..2644
                     /locus_tag="VFV20_08425"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEU5288414.1"
                     /translation="MTETVSPRRLRVIFAAFACATILLSLRVGYWQTVGRAALLEGAT
                     DQVRSDLVLAAQRGVVRDRNGALLATTVPLRSLYAIPGRIPDHAAAAAKLAPLLGTTS
                     DAVQRALDSGAEWLYLRRRLPEATAKAIEMLGISGLGFETEPKRLYPNDAIGAHVLGF
                     VNDDGAGQYGVEGAYDAVLRGVPGRLVVERDPKDRELAVGLRTATPPVNGADLTLTID
                     LVLQTSVERNLEAACAKEHCASGSMIILDPRSGAILALASWPSYDPAAVAKADPKTLL
                     DRAVSWTYEPGSTMKAITIAAAIDRHVVTPNTTYEDKGYAVIGGRVLHNALGKSYGTS
                     TVTQILEHSANAGAVFVAQKLGAEQLRAYLEAFGFGVPSGVDLSGEAAGDVRPLAEWY
                     PVDLGTIAFGQGIAATPMQLAAAYAAIANGGTLYKPYVVAARRDADGEHRTAPVAVRR
                     PISPETAATLRMMLTSTVDNGIANGAAIKGFSVAAKTGTAQIPSDDGRYVDDAYVSSL
                     AGFFPANDPRFVAVIVLERPQSGLLGTVTAMSAFRSVAVDALRNARVQPDRP"
     gene            2664..4040
                     /gene="murF"
                     /locus_tag="VFV20_08430"
     CDS             2664..4040
                     /gene="murF"
                     /locus_tag="VFV20_08430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEU5288415.1"
                     /translation="MFTLVDLLHATGGQVIHGNASGDERFNGGAFDSRVVRPGEVFFA
                     LRDQRDGHDFVADAFARGASAAVVERSVDGLPADALVLRVGSPLGALRALAELLRERH
                     PIPAIGITGNVGKTTAKEATAAALGARYRVLKSAASYNNEIGVPLTFLAIEPSHDAAV
                     IELGFYVPGEIADLCRLVRPRVGVITRIPDKPVHFSRTPSVEAIAAGKAELIASLPED
                     GIALLNIDDPRVRALASRTRARVVLYGEAEGAELRATDVRADGIEGIRFVATYGGERA
                     QVALPLPGRHVVVAALAALGSARALGVPLEEAAIAMATLERPRHRMEVRSAGRLTVID
                     DSYNASPAAVIAALAVLREARGRRVAVIGDMKELGTLTGESHDEVGREAARSADVLVG
                     VGELARIAVESAKRAGLRETHGVADAAEALVLLKRIQKAGDVILVKGSNSLRLWEVAD
                     ALVGTVPV"
     gene            4040..5029
                     /gene="mraY"
                     /locus_tag="VFV20_08435"
     CDS             4040..5029
                     /gene="mraY"
                     /locus_tag="VFV20_08435"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU5288416.1"
                     /translation="MAILALGVLLAAFLLGLVLGAPYIRLLRRLRMGKQVRREGPASH
                     LVTKQGMVTMGGVLFIFVTFVLWLLVLILLPPEERDDYLPQTIVPIGTLIAVGILGFI
                     DDYVNVLYGFGIRGRHKLVWQTIVALAAALYIQRHFNITGVYVPLYGEVIIGPVLFVA
                     LAVFAIVAMSNGMNLTDGLDGLAGGTAVFAFLSFAFVAAARGFPWLVVFCSAMVGALL
                     AYLWYNVHPADFMMGDVGSLALGATLTTIALTTGFILLLPIAGVIFVAETVSVVLQVG
                     SFKLRGKRIFRMSPLHNHFELIGWPEEKTTIRFWLIGALAAIVAAILAFATPK"
     gene            5035..>5312
                     /locus_tag="VFV20_08440"
     CDS             5035..>5312
                     /locus_tag="VFV20_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288417.1"
                     /translation="MRRDAAAGDTGQAGAWLARDGLLWSPALAAIGVVAGFTTRAQGS
                     MAGSHHSLEAQAANRAALAERLGFDAVVRVKQVHGRDIVRVDGPVEPWP"
BASE COUNT          703 a         1907 c         1910 g          792 t
ORIGIN      
        1 atcgtgaaca gctggggcga gcgtgagctg cgtcgcgtct tctccgactt cggagaggag
       61 ccggaggccg ggcggatcgc cgcggccatc gtgcgccgcc gctcgcgcga gccgttcctc
      121 accgccgacg acctcggccg cttcgtgttc ggcgtcaagc ggcagcgctc tcgtgcgatc
      181 gatcccgcga cgcgcgtctt ccaggcgctg cgcatcgcgg tcaacgacga gctcgcgaat
      241 ctggagcgcg gcctcgtggc cgcgttgtcg ctcctgcgtc ccggtggccg cctcgcggtc
      301 atctcgttcc actcgctcga ggaccgcgcc gtgaagggat tcatggcgcg tgaatcgcgc
      361 gactgcatct gtcctcccca cctccccacc tgcgtctgcg ggcaccgcgc gggtctgcgt
      421 gtcgttaccc ggcgtccgct tcgtgcggac tcggcggagg tcgaccgcaa cccgcgcgcc
      481 cgcagcgcag cccttcgagt cgcggaaaag atcgcctcgt gatcgcgtcc caggccgtcc
      541 gcgcggcgat gccgcgccgg gtggtgcgcc cgcgtccccg ccacgcgcag cgcctcgcgc
      601 gcgcgtccgc acgtgcgacg ttcgaggtgc ggaccttcgc gggcgtgagc gcggccatcg
      661 cgatcgcgtt cttcctctcg atgctctacc tcgcgcaggc gacgacggtc tcgaccctcg
      721 gctaccaggc gcagcacctg cagcaggtcc gtgacgagct ccgcaggcag aacgcgctcc
      781 tcgaggtcga gaacgcccgt ctggattcgc ccgcacgcat cgagaccgag gccgcgaagc
      841 tcgggctcgt tcgcgccgcg cagataccgg tcgtccagct cgaaccgatc accgcgaagc
      901 gatagcgccg gacgggtgct gctggggagc gagcaccctg ccggcctgag gagagggaca
      961 ggtgacggag acggtctccc cgcggcgact gcgcgtcatc ttcgcggcat tcgcctgcgc
     1021 caccatcctg ctgtccctcc gcgtgggcta ctggcagacc gtcggacgcg cggcgctcct
     1081 ggagggcgcc accgaccagg tgcgcagcga tctcgtcctc gcagctcagc ggggcgtggt
     1141 gcgcgaccgg aacggcgcgc tgctggcgac gaccgtgccg ctccgctcgc tctatgcgat
     1201 cccggggcgc atccccgatc acgccgccgc ggccgcgaag ctcgccccgc tcctcggcac
     1261 gacgagcgac gccgtgcagc gcgcgctcga ctccggcgcg gagtggctgt acctgcggcg
     1321 ccgcctgccg gaagcgacgg cgaaggcgat cgagatgctg ggcatcagcg ggctcggctt
     1381 cgagacggag cccaagcgcc tgtatccgaa cgacgcgatc ggcgcccacg tgctcggctt
     1441 cgtgaacgac gacggcgccg gccagtacgg ggtcgaaggt gcctacgacg cagtcctgcg
     1501 tggcgtcccc gggcggctcg tcgtcgagcg cgatccgaag gatcgcgagc tcgccgtcgg
     1561 cctccggacg gcgacgccac cggtgaacgg cgcggacctc acgctcacga tcgacctcgt
     1621 cctccagacc tccgtcgaac ggaatctcga ggcggcgtgc gcgaaggagc actgcgcgag
     1681 cgggtcgatg atcatcctcg atccgcgcag cggcgcgatc ctggcgctgg cgtcgtggcc
     1741 cagctacgac ccggcggcgg tggcgaaggc ggacccgaag acgctgctcg accgcgccgt
     1801 ctcgtggacc tacgaaccgg gctcgacgat gaaggcgatc accatcgcgg cggcgatcga
     1861 tcggcacgtc gtgacgccga acaccaccta cgaggacaag gggtacgcgg tcatcggcgg
     1921 acgcgtgctg cacaacgcgc tcggtaagtc gtacggcacc tcgaccgtga cacagatcct
     1981 cgagcactcc gcgaacgccg gcgccgtgtt cgtcgcccag aagctcggcg cggagcagct
     2041 gcgcgcgtac ctcgaagcgt tcggcttcgg ggtgccgagc ggcgtcgatc tctcgggcga
     2101 ggcggcgggc gacgtgcgcc cgctcgcgga gtggtatccg gtggacctcg gcacgatcgc
     2161 gttcggccag ggcatcgcgg caacgcccat gcagctcgcg gcggcgtacg cggcgatcgc
     2221 gaacggcggg accctctaca agccgtacgt ggtggcggcg cggcgcgatg cggacgggga
     2281 gcaccgcacc gcgcccgtcg ccgtgcggcg tccgatctcg ccggagaccg cggccacgct
     2341 ccgcatgatg ctcacgtcga cggtggacaa cggcatcgcg aacggcgcgg cgatcaaagg
     2401 cttcagcgtc gccgcgaaga cggggaccgc gcagatcccg agcgacgacg gccgctacgt
     2461 cgacgacgcg tacgtgtcgt cgctggccgg attcttcccg gcgaacgatc cgcgcttcgt
     2521 cgcggtgatc gtgctggagc ggccgcagtc cgggctgctc ggcacggtga ccgcgatgtc
     2581 cgcgttccgg agcgtcgcgg tggacgcgct gcggaacgcg cgcgtgcagc cggaccggcc
     2641 ataggcgtcg gtaccatcct ctggtgttca cgctcgtcga cctgctgcac gcgacggggg
     2701 gccaggtgat tcacggcaac gcctccggcg acgagcgctt caacggcggc gcgttcgact
     2761 cgcgcgtggt ccggccgggc gaggtcttct tcgccctgcg cgaccagcgc gacgggcacg
     2821 acttcgtcgc cgacgcgttc gcgcgcggcg cgagcgcggc cgtcgtggaa cggtccgtcg
     2881 acggacttcc ggccgacgcg ctcgtgctgc gcgtcggctc gccgctcggg gcgctgcgcg
     2941 cgcttgcgga gctgctgcgg gagcgtcacc cgatcccggc gatcggtatc accggcaacg
     3001 tggggaagac caccgcgaag gaggcgaccg ccgcggcact gggcgcgcgc taccgcgtgc
     3061 tgaagagcgc cgcctcctac aacaacgaga tcggggtacc gctcacgttc ctcgcgatcg
     3121 agcccagtca cgacgcggcg gtgatcgagc tcgggttcta cgtgccgggc gagatcgccg
     3181 acctctgccg gctcgtgcgc ccgcgcgtgg gcgtcatcac gcgcatcccc gacaagcccg
     3241 tgcacttctc gcgcacgccg agcgtcgagg cgatcgccgc gggcaaggcg gagctcatcg
     3301 cctcgctgcc cgaggacggg atcgcgctgc tgaacatcga cgacccgcgc gtgcgcgcgc
     3361 tcgcgtcgcg cacccgcgcg cgggtggtgc tgtacggcga ggccgagggc gcggagctcc
     3421 gcgcgacgga tgtgcgcgcc gacgggatag agggcatccg cttcgtcgcg acctatggcg
     3481 gcgagcgcgc gcaggtggcg ctcccgctcc ccggccgcca cgtcgtggtc gcggcgctcg
     3541 cggcgctcgg gagcgcgcgc gctctcggcg tgccgctcga ggaggcggcg atcgcgatgg
     3601 cgacgctcga gcgaccgcgg caccgcatgg aggtgcggtc ggccggccgg ctcacggtga
     3661 tcgacgacag ctacaacgcc agccccgcgg ccgtcatcgc ggcgctcgcc gtgctgcgcg
     3721 aggcgcgcgg ccggcgcgtc gccgtcatcg gcgacatgaa ggagctcggc acgctgaccg
     3781 gtgagtcgca cgatgaggtc gggcgcgagg cggcgcgcag cgcggacgtg ctggtcggcg
     3841 tgggcgagct cgcgcgcatc gccgtcgaga gcgcgaagcg cgccggtctg cgcgagacgc
     3901 acggtgtcgc cgacgccgcg gaagcgcttg tcctgttgaa gcggatccag aaggcgggcg
     3961 acgtgatcct cgtcaaggga tcgaactcgc tccggctctg ggaggtcgcc gacgccctcg
     4021 tgggcacggt gccggtctga tggccatcct cgccctcggg gtcctgctcg ccgcgttcct
     4081 gctcgggctc gtgctcggcg ccccgtacat ccgcctgctg cggcggcttc ggatgggcaa
     4141 gcaggtccgg cgcgaggggc cagcgtcgca cctggtcacg aagcagggca tggtcacgat
     4201 gggcggcgtc ctcttcatct tcgtgacgtt cgtgctgtgg ctgctcgtcc tcatcctgct
     4261 cccgccggag gagcgcgacg actacctgcc gcagaccatc gtcccgatcg gcacgctcat
     4321 cgcggtgggg atcctcggct tcatcgacga ctacgtgaac gtcctgtacg gcttcggcat
     4381 ccggggccgg cacaagctcg tctggcagac gatcgtcgcc ctggcggcgg cgctgtacat
     4441 ccagcgccac ttcaacatca ccggcgtcta cgtgccgctc tacggcgagg tgatcatcgg
     4501 gccggtgctc ttcgtcgcgc tcgcggtgtt cgccatcgtc gcgatgtcca acgggatgaa
     4561 tctcaccgac ggcctcgacg gcctcgccgg cggcaccgcg gtcttcgcgt tcctgtcgtt
     4621 cgcgttcgtc gcggccgccc gcggattccc gtggctcgtc gtgttctgca gcgcgatggt
     4681 gggcgccctg ctcgcgtatc tctggtacaa cgtccacccc gcggacttca tgatgggcga
     4741 cgtcggctcg ctcgcgctcg gcgccaccct cacgaccatc gcgctcacga ccggcttcat
     4801 cctgctgctc ccgatcgccg gcgtgatctt cgtcgcggag acggtctcgg tggtcctgca
     4861 ggtcggttcg ttcaagctgc gcggcaagcg catcttccgc atgtcgccgc tgcacaacca
     4921 cttcgagctc atcgggtggc cggaggagaa gaccaccatc cggttctggc tcatcggagc
     4981 gctcgccgcg atcgtcgccg cgatcctggc gttcgcgacg ccgaagtgag cgcgatgcgg
     5041 cgcgacgccg cggcgggcga cacgggccag gccggcgcgt ggctcgcgag ggacggcctg
     5101 ctctggtcgc cggccctcgc ggcgatcggc gtcgtcgccg gcttcacgac gagagcgcag
     5161 ggcagcatgg ccgggtcgca tcactcgctc gaagcgcagg ccgcgaatcg cgcggcgctc
     5221 gcggagcggc tgggattcga cgcggtcgtg cgcgtgaagc aggtgcacgg acgagacatc
     5281 gtccgcgtcg acggcccggt ggagccgtgg cc
//
LOCUS       DASTZR010000376         2213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_647458, whole genome shotgun sequence.
ACCESSION   DASTZR010000376 DASTZR010000000
VERSION     DASTZR010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647458"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1398
                     /locus_tag="VFV20_08445"
     CDS             <1..1398
                     /locus_tag="VFV20_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU5288418.1"
                     /translation="TDMAQRTILVADDDAAIRSLLKQVLGDEGYAVVEAATGTEVLEG
                     VKTSNPDLVIMDVRMPELDGIEALQKVKTLSPETAVLIMTAFGTSNAAIKAMELGAFD
                     YITKPFELDKISHTVKRAFQYQDLTAEVEVLRDEISSLVQTERIVGNSSAMQEVYKTV
                     GKVAKSDATVLITGESGTGKELVAEALHYNSTRRSGPLVKVSCAALPETLLEAELFGH
                     EKGSFTGATAMRKGRFELADKGTIFLDEIGEMSLATQTKLLRVLQERKIERVGSAIPI
                     KVDIRVICATNKDLQKQVEQTKFRDDLFYRLNVINIHMPALRDRKEDIPALVEHFLAK
                     HRYSASAQPASISEEALKRLMEYDWPGNVRELENVVERAVVLSRGQIITSRELPFGDH
                     EAGDQGDEGDDIAMEKSFFKKSVAQFEKDMIMKALRDAGGNRSKAAEMLGIYRRLLYA
                     KIKEFGLEGYPPKGR"
     gene            1408..1659
                     /locus_tag="VFV20_08450"
     CDS             1408..1659
                     /locus_tag="VFV20_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU5288419.1"
                     /translation="MFGIFRIALRSFALGVAVGVLFAPRSGAETRRMLGERFAMAMDG
                     LVDFAALPPVPPERARTNGHAERTAGKKPRTSLDARSSS"
     gene            1685..1921
                     /locus_tag="VFV20_08455"
     CDS             1685..1921
                     /locus_tag="VFV20_08455"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin domain-containing protein"
                     /protein_id="HEU5288420.1"
                     /translation="MSSRRPAPASVVRLTEATGSSPRGWEAAVAAAVKASEVRAPIGV
                     EVSRFWAEFDGRKLNRYHVTVKVAYRQSLKAASR"
BASE COUNT          398 a          729 c          746 g          340 t
ORIGIN      
        1 acagacatgg cccagaggac cattctcgtt gcggacgacg acgccgcgat ccgatcactt
       61 ctgaagcagg tcttgggcga cgaaggctat gccgtcgtcg aggcggccac gggcaccgag
      121 gtcttggagg gcgtgaagac ctccaacccg gacctcgtga tcatggacgt gcggatgccg
      181 gagctcgacg gcatcgaggc gctccagaag gtgaagaccc tgtcgccgga gacggcggtc
      241 ctgatcatga cggccttcgg cacctcgaac gcggccatca aagccatgga gctcggcgcc
      301 ttcgactaca tcaccaagcc gttcgagctc gacaagatct cgcacacggt gaagcgcgcc
      361 ttccagtacc aggacctcac agccgaggtc gaagtgcttc gcgatgagat cagctcgctc
      421 gtgcagacgg agcgcatcgt cggcaactcg tcggcgatgc aggaggtcta caagaccgtc
      481 ggcaaggtcg cgaagtccga cgccacggtc ctgatcaccg gcgagagcgg cacgggcaag
      541 gagctcgtcg ccgaggcgct gcactacaac tcgacgcgtc gcagcgggcc gctggtcaag
      601 gtgtcctgcg cggcgctgcc ggagacgctc ctcgaggccg agctcttcgg tcacgagaag
      661 ggctcgttca ccggcgcgac ggccatgcgc aagggccgct tcgagctcgc cgacaagggc
      721 acgatcttcc tcgatgagat cggcgagatg agcctcgcca cgcagacgaa gctccttcgt
      781 gtcctccagg agcgcaagat cgagcgcgtc ggatccgcca tcccgatcaa ggtcgacatt
      841 cgcgtgatct gcgcgaccaa caaggatctg cagaagcagg tcgagcagac gaagttccgc
      901 gacgacctct tctaccgcct caacgtgatc aacatccaca tgccagcgct gcgggaccgc
      961 aaggaggaca tccctgcgct cgtcgagcac ttcctcgcga agcaccggta cagcgccagc
     1021 gcgcaaccgg cgtcgatcag cgaggaggcc ctgaagcgcc tcatggagta cgactggccg
     1081 ggcaacgtgc gcgagctcga gaacgtcgtc gagcgcgctg tcgtgctgtc gcgcgggcag
     1141 atcattacca gccgcgagct ccccttcggc gaccatgagg ccggcgacca gggggacgag
     1201 ggcgacgaca tcgcgatgga gaagtccttc ttcaagaagt ccgtcgcgca gttcgagaag
     1261 gacatgatca tgaaagccct tcgcgacgcc ggcgggaacc gttcgaaggc cgcggagatg
     1321 ctgggcattt atcgtcgcct gctgtacgcg aagatcaagg agttcggcct cgagggctat
     1381 ccgcccaagg gccggtagga ggtcgcgatg ttcggcatct tccgcatcgc gctgcggtcc
     1441 ttcgcgctcg gtgtcgccgt cggcgtcctg ttcgcgcccc gaagcggcgc cgagacgcgt
     1501 cggatgctcg gcgagcgctt cgcgatggcg atggacgggc tggtcgactt cgccgcgctg
     1561 cccccagtgc cgccggagcg cgcgcggacg aatggccacg ccgagcgcac cgcggggaag
     1621 aagccgcgca cgagcctgga tgcccgctcc tcgtcgtagt cgtacggcct cgtcgagccg
     1681 ccgcgtgagc tcgcgccgtc cggcgccggc gtcggtggtg cgactgaccg aggcgaccgg
     1741 ctcgtccccg cgcgggtggg aggcggcggt cgccgccgcg gtgaaggcga gcgaggtgcg
     1801 cgctccgatc ggcgttgagg tgtcccggtt ctgggcggag tttgatggac gcaagctgaa
     1861 ccgctaccac gtgaccgtga aggtggccta ccgccagtcg ctaaaagccg cttcccgcta
     1921 gccctgcgtg ggtctcaaaa cgccatctgt ctcggttctt gacacgcacg cccccggcca
     1981 ccgaacattc tcccgggcat cggcgtttga cacgccggac gggcaaaggg gagcaagcgt
     2041 ggccgaggtc gagacccggg aaattctggc gcgcatcctc atcgcgaacg acggggaggt
     2101 cctctcgcgc ctccgtctgt ccgcggccga agccgcgcga gtctgcaacg tcacgcctcg
     2161 ccagctcatc tattggacca agaagggcct cgtcaaggcg tccgcggacg gtg
//
LOCUS       DASTZR010000377         3791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_961290, whole genome shotgun sequence.
ACCESSION   DASTZR010000377 DASTZR010000000
VERSION     DASTZR010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961290"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VFV20_08460"
     CDS             <1..783
                     /locus_tag="VFV20_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288421.1"
                     /translation="LRVPMTIMWNPRAWTTDQVSPSRVEAMLAWTKGRARTGDEVALH
                     IHAWTDFVRAAGVTPRTSPSWSGRGDGYDVPLTAFTEDETKRLLDHALKLMAEHGLPR
                     PTTFRAGGLFANAANLRAVAAAGFTADTSGAPAGPFGRLPLPWTLAKDAQPYHPSRDD
                     ANAAGDLPLLEVPNIAGNTYGYTVTSIRRTASDDIAMLAPVTEVAVQRRVLTLVSHPA
                     TIDETERAAIESLLRAFEPYRYDRDAGPLRFVTMSQVAQAFR"
     gene            complement(839..2761)
                     /locus_tag="VFV20_08465"
     CDS             complement(839..2761)
                     /locus_tag="VFV20_08465"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5288422.1"
                     /translation="MQQQQTSVPSSTVTTVPAAPPAPTPVQSFLMTTAYVLEHNLRRL
                     IILAVIFGIALSLYGIRGIFQGILDMIGQAPQLLVLLLFYSFMMIVQFGALMWFMSRP
                     RTYTVTPDSPQIGLSFENYRGQPDLLDHAKSLVRILKGVKSFQDRGGEMPQGMLLAGP
                     PGTGKTFLAGVMAAEAQLPFIYVDASSLSSMWMGVDALIVISLFRKARGLARKFAAPG
                     QPGCCILFLDELDSVGLSRGGMSGGQSQGMMGPMGMMGGRGLALNTMLNQMDSLGKHV
                     EDRFKYKVLRWLGVVRGPVPPKPVVFVIGATNRPDVLDPALVRAGRLDRKLMVYEPDG
                     EGRRDILQHYLRQKAHEPNIPVDLMVADSIGWTPIELKTVINEALIVAHDAGRDYLTY
                     KDWLTARDARLLGIKQPILSMSDHDRRTIAYHEAGHAIASHYLNKDDRIQKASIIRMG
                     DAYGVVMPTPKLERHQLHAEEIENDIMVSLGSRAVEELILRTKTANASSDLRNATNRA
                     LAYVGTWGMGSTLLAFPPSAAGAPPQVLSLADKLLEALYEETKRLIREKEYAVHAIAG
                     ALLQKGELIGPELDEIFDAADLSNPEAAKPFVRKPVTLPKMADLMKEEHQSPAVAVVP
                     VAASELPPSPSPTPEG"
     gene            complement(2761..3177)
                     /locus_tag="VFV20_08470"
     CDS             complement(2761..3177)
                     /locus_tag="VFV20_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288423.1"
                     /translation="MSEARAVLVLALFIGGLATGVFVQRVTDGGGRENPYPSLDRVEE
                     PAQTAAVATALMNNDAKGLAGLLDSQTLQELKDALMSPTGAPVADIRSIKFAGATSKA
                     GRTLAGYIVTGKDVQGADAIVGFVLNIENGQIVGVN"
     gene            complement(3174..3605)
                     /locus_tag="VFV20_08475"
     CDS             complement(3174..3605)
                     /locus_tag="VFV20_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288424.1"
                     /translation="MGKVFSWVPGIVRVAIGMVAVGAMIGYVLSAVVTTVINDQGWFA
                     TTGESPQARGYMVALLQREADSLNALRPQQDVVSQAMAQQAAQQSRQQAKPLSLTYLG
                     GGSQGRLSVQIYAVEVRANDGSQQLFPLALTLLGGKVVETR"
BASE COUNT          574 a         1277 c         1336 g          604 t
ORIGIN      
        1 ctgcgggtgc cgatgacgat catgtggaac ccccgcgcgt ggacgacgga ccaggtctcg
       61 ccgtcgcgcg tggaggcgat gctcgcatgg acgaagggcc gcgcgcggac gggcgatgag
      121 gtggcgctgc acatccacgc ctggaccgac ttcgtgcgcg cggcgggtgt gacgccgcgt
      181 acgtcgccga gctggagcgg gcgcggcgac ggctacgacg tcccgctcac cgcgttcacc
      241 gaggatgaga cgaaacggct cctcgatcac gcgctgaagc tcatggccga gcatggcctc
      301 ccgcggccga cgaccttccg ggccggcggc ctgttcgcga acgcggcgaa cctgcgcgcg
      361 gtcgcggcgg ccgggttcac cgccgacacg agcggtgcgc ccgccgggcc gttcggccgg
      421 ctgccgctgc cgtggacgct ggcgaaggac gcacagccct atcacccgtc gcgcgacgac
      481 gcgaacgccg ccggcgatct cccgctgctc gaggtcccga acatcgcggg caatacgtac
      541 ggctacaccg tgacgagcat ccgccggacc gcgagcgacg acatcgcgat gctcgcaccg
      601 gtcaccgagg tcgccgtcca gcggcgcgtg ctcaccctcg tctctcaccc ggccacgatc
      661 gacgagaccg agcgcgcggc gatcgagtcc ctgctccgcg ccttcgagcc ctaccgttac
      721 gacagggacg ccggaccgct ccggttcgtc acgatgtcgc aggtcgcgca ggcgttcagg
      781 tagaacgaag gccggcccgt tgggccggcc tcgctcgaat gcggatccgc gcgacgggtc
      841 agccttcggg tgtcggcgac ggtgagggag gaagctcgga ggccgcgacc ggcacgaccg
      901 cgacggcggg gctctggtgc tcctccttca tgaggtcggc catcttcggc agcgtcacgg
      961 gcttccggac gaacggcttc gccgcctccg ggttcgagag atccgcggcg tcgaagatct
     1021 cgtcgagctc ggggccgatg agctcgccct tctggagcag cgcgcccgcg atcgcgtgga
     1081 ccgcgtactc cttctcgcgg atgagccgct tcgtctcttc gtacagggcc tcgaggagct
     1141 tgtcggcgag cgacagcacc tgcgggggcg cgcccgcggc gctcggcggg aacgcgagga
     1201 gcgtcgaccc catgccccac gtgccgacgt acgcgagcgc gcggttcgtc gcgttgcgga
     1261 ggtccgacga cgcgttcgcg gtcttcgtcc gcaggatgag ctcctcgacg gcgcgcgaac
     1321 cgagcgagac catgatgtcg ttctcgatct cctcggcgtg gagctgatgg cgctcgagct
     1381 tcggcgtggg catcacgacg ccgtacgcgt cgcccatgcg gatgatcgag gccttctgga
     1441 tgcggtcgtc cttgttcagg tagtgcgacg cgatggcgtg gccggcctcg tggtaggcga
     1501 tcgtgcggcg gtcgtggtcg gacatgctca ggatcggctg cttgatgccg aggaggcgcg
     1561 cgtcacgcgc ggtcagccag tccttgtacg tcaggtaatc gcggccggcg tcgtgcgcga
     1621 cgatgagcgc ctcgttgatc acggtcttga gctcgatggg cgtccagccg atcgagtccg
     1681 cgaccatcag gtccacgggg atgttcggct cgtgcgcctt ctgccgcagg tagtgctgca
     1741 ggatgtcgcg gcggccctcg ccgtccggct cgtagaccat gagcttgcgg tcgagacggc
     1801 cggctcgcac gagcgccggg tcgagcacgt cgggacggtt cgtcgcaccg atgacgaaga
     1861 cgaccggctt cggcggtacg gggccgcgca cgacaccgag ccagcgcagg accttgtact
     1921 tgaagcggtc ttcgacgtgc ttccccagtg agtccatctg gttgagcatc gtgttcaggg
     1981 cgagcccgcg gccgcccatc atgcccatcg ggcccatcat tccctgcgac tggccgccgg
     2041 acatgccgcc gcgcgacagg cccaccgagt cgagctcgtc gaggaacagg atgcagcagc
     2101 cgggctggcc gggcgccgcg aacttgcgcg ccaggccgcg cgccttgcgg aagagcgaga
     2161 tgacgatgag cgcgtcgacg cccatccaca tgctcgagag gctcgacgcg tcgacgtaga
     2221 tgaacggcag ctgcgcctcg gcggccatga cgccggcgag gaatgtcttg ccggtgccgg
     2281 gcgggcccgc gagcagcatg ccctgcggca tctcgccacc gcggtcctgg aacgacttca
     2341 cgcccttcag gatccggacg aggctcttcg cgtgatcgag gagatcgggc tggccgcggt
     2401 agttctcgaa cgagagcccg atctgcgggc tgtccggcgt gaccgtgtag gtgcgcgggc
     2461 gcgacatgaa ccacatcagc gcaccgaact gcacgatcat catgaacgag tagaagagga
     2521 gcaggacgag gagctggggc gcctgtccga tcatgtcgag gatcccctgg aagatgccgc
     2581 ggatcccgta caggctcagg gcgatcccga agatgacggc gaggatgatc agccgtcgca
     2641 ggttgtgctc gagcacgtag gccgtcgtca tgaggaagga ctgcacgggc gtgggggccg
     2701 gcggcgccgc gggcaccgtg gtgaccgtgc tcgacgggac gctcgtctgc tgctgttgca
     2761 tcagttcaca cctacgatct ggccgttttc gatgttgagc acgaagccca cgatggcgtc
     2821 ggcaccttgc acgtccttgc cggtgacgat gtagccggca agcgtacgtc cggccttcga
     2881 tgtcgccccg gcgaacttga tgctgcggat gtcggcgacc ggcgccccgg tcggtgacat
     2941 gagcgcgtct ttgagctcct gcagtgtctg gctgtccagg aggcccgcga ggcccttggc
     3001 gtcgttgttc atgagcgccg tcgcgacggc cgccgtctgg gccggctcct cgacgcgatc
     3061 caggctcgga taggggttct ctcgaccgcc accgtcggtg acccgctgga cgaagacacc
     3121 cgtggcgagg ccgccgatga agagcgcgag cacgagcacg gcgcgcgctt cgctcaccgg
     3181 gtttcgacga ccttgccgcc cagcagcgtg agcgcgagcg ggaagagctg ctgcgatcca
     3241 tcgttcgcgc gcacttcgac cgcgtagatc tggacgctga gccttccctg ggagcccccg
     3301 ccgaggtagg tcagcgacag tggcttcgcc tgctgacggc tctgctgcgc ggcctgctgg
     3361 gccatggcct gcgacacgac gtcctgctgc ggccgaaggg cgttgaggct atcggcctcg
     3421 cgctgcagca gcgccaccat gtacccgcgg gcctgcggcg actcccccgt cgtggcgaac
     3481 cagccctggt cgttgatgac ggtcgtgacc accgccgaca ggacgtaccc gatcatcgcg
     3541 ccgaccgcga ccatgccgat cgcgacgcgg acgatccctg ggacccagct gaagaccttt
     3601 cccacgtatg aagtctatct ggcgtctaac ggaaaaaggt tcatccggcc cgtttcagga
     3661 cgcccatcgc gaatgccagc agacctgaga gctcccgcgc ccagaagtcc gggccctgtt
     3721 gacgcgcccc tgtgtcgagc gccgcagcgt gcgagagcag ccgagtgagc tggagacggg
     3781 tctctccctc g
//
LOCUS       DASTZR010000378         2184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1248419, whole genome shotgun sequence.
ACCESSION   DASTZR010000378 DASTZR010000000
VERSION     DASTZR010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248419"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="VFV20_08480"
     CDS             complement(<1..1122)
                     /locus_tag="VFV20_08480"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEU5288425.1"
                     /translation="MSLEPILALIVVAVMANLVVMGVLVASPTVGRIRSGRGADDAPD
                     VRTERALAAAAVVGRGDDARTADDGETIRTYDRVVRIASWVFLLAVTTIVGVTGLWPE
                     SQSAIFLLLALGGVFVLVIHDLLPATALGPAKFVLEGSVAITVATVLVALTGGATSPF
                     FFVYPLIVGGAALVVSPPVTLGLAITAAIGYLLAVTLGSPSDALSDPITVATVGINLT
                     AMVLLAYIAMVIAREQRSTRDAAIRLSTIDPLTSLFNRGFFFAALEREIARSARSGRG
                     FCLLMMDLDELKAINDRLGHFHGDRVLAAVGQVVSDGVRRIDTAARYGGDEFVVLLPE
                     TDPTGAFVLAEKIRIGVNDLELDLPEEAVRPSLSVGVVSY"
     gene            complement(1240..1737)
                     /locus_tag="VFV20_08485"
     CDS             complement(1240..1737)
                     /locus_tag="VFV20_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEU5288426.1"
                     /translation="MTDVARPRPRAISTRADEVSDLDKRIIEHLQADGRRPFTQIAAE
                     LGVSEAAVRARTNRLIERGILQVVGVTDPLKLGFQQMAMIGIRCESDRLVAVAEAVAE
                     MPEVDYVVITAGTYDLLIETVCEDNEALLRFLTERLRSIEGVRETETFVYLRMVKQTY
                     QWGTR"
     gene            complement(1805..>2184)
                     /locus_tag="VFV20_08490"
     CDS             complement(1805..>2184)
                     /locus_tag="VFV20_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288427.1"
                     /translation="TATLGADGTDVVTKIFATYVGWPLDVTEYRSAKALSEAVKWPDG
                     EAPGKGEPPITLAGMNILITWGPTRSGHTPGKLNGRQVEALTNLVSAADVLLSPLRTR
                     SSVPVKVTAPKPAASAAASPAAS"
BASE COUNT          355 a          732 c          774 g          323 t
ORIGIN      
        1 ataggacacg acgccgaccg acaatgacgg ccggaccgct tcctcgggca ggtcgagctc
       61 gaggtcgttc acgccgatgc ggatcttctc ggcgaggacg aacgcgcctg tcgggtcggt
      121 ctcggggagg agcacgacga actcgtcgcc gccgtaccgt gcggccgtgt cgatccggcg
      181 cacgccgtca ctcacgactt gtccgaccgc ggcgagcacc cggtcgccgt ggaagtgccc
      241 gagtcgatcg ttgatcgcct tgagctcgtc gaggtccatc atcagcaggc agaagccacg
      301 cccggaccgc gcgctgcgcg cgatctcacg ctcgagcgct gcgaagaaga aaccgcggtt
      361 gaagagcgat gtcagcgggt cgatcgtcga caggcggatc gcggcatcac gcgtgctccg
      421 ctgctcacgg gcgatgacca tcgcgatgta cgcgagcagg accatcgcgg tgaggttgat
      481 cccgacggtc gcgaccgtga tcgggtcgct cagcgcgtca gatggcgacc cgagcgtcac
      541 ggccagcagg tagccgatcg ccgcggtgat cgccaggccg agcgtgacgg gtggtgagac
      601 gacgagcgcg gccccaccca cgatcagcgg atagacgaag aagaagggac tcgtggcacc
      661 gccggtgagc gcgacgagga ccgtcgccac cgtgatcgcg acggaccctt cgagcacgaa
      721 cttcgccggg ccaagcgcgg tcgccggcag gaggtcgtgg atgacgagca cgaacacgcc
      781 acccagggcg agcaggagga agatcgcgct ctggctctcg ggccagagtc ccgtgacacc
      841 cacgatcgtc gtgacggcga gcaggaaaac ccacgacgcg atgcggacga cccggtcgta
      901 tgtccggatc gtctcgccgt catccgccgt cctggcgtcg tcaccacggc cgaccacggc
      961 ggcggccgcg agggcgcgct ccgtgcgaac gtcgggtgcg tcgtcagcgc ccctccccga
     1021 gcggatgcga ccgacggtcg gcgaggcaac gaggacaccc atcaccacga gattcgccat
     1081 gacggcgacg acgatcaggg caaggatggg ctccagcgac acgtggccat cttagaccgg
     1141 gtcctaggag attcggccgg tgcacgctgg cgccacccga acggcgcggt gcacccacgc
     1201 gacggtgtca cgcgctggga ggtgcgtcca ggcgacgctc tagcgcgtcc cccactggta
     1261 ggtctgcttg accatccgca ggtacacgaa cgtctcggtc tcccggacac cctcgatcga
     1321 acgaagccgc tcggtcagga accggaggag cgcctcgttg tcctcgcaca cggtctcgat
     1381 cagcaggtcg tacgtgccgg cggtgatcac gacatagtcg acctcgggca tctcggcgac
     1441 cgcctcggcg accgccacca gccggtcgct ctcgcagcgg atgccgatca tcgccatctg
     1501 ctggaagccg agcttgagcg ggtcggtcac accgacaacc tggaggatgc ctcgctcgat
     1561 caggcggttc gtgcgagcgc ggaccgccgc ctcgctgaca cccagttctg cggcgatctg
     1621 cgtgaagggg cgcctcccgt ctgcctgcag gtgctcgatg atccgcttat cgaggtccga
     1681 cacctcgtcg gcgcgggtcg agatcgctcg cggtcgcggc cgggcgacgt cggtcatggc
     1741 gacagagtac ggcgtcaggg tcgttccggg accacgttcc gtggccgcgt cccggagggc
     1801 ggtctcagga cgctgcgggc gaggcagcgg cgctcgctgc gggcttcggg gcggtgacct
     1861 tgacgggcac gctcgagcgg gtccggagcg gcgagaggag cacgtccgcg gcgctcacga
     1921 ggttcgtcag tgcttccacc tggcggccgt tgagcttgcc cggggtgtgc ccggatcggg
     1981 tcgggcccca cgtgatgagg atgttcatgc cggcaagggt gatcggcggc tcgcccttgc
     2041 ccggtgcctc gccgtcgggc cacttcacgg cctcgctgag ggccttggcg gagcggtact
     2101 cggtgacgtc caacggccag ccgacgtagg tcgcgaagat cttcgtgacg acatcggtcc
     2161 catcggcgcc cagggtcgcg gtgt
//
LOCUS       DASTZR010000379         5034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1039487, whole genome shotgun sequence.
ACCESSION   DASTZR010000379 DASTZR010000000
VERSION     DASTZR010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039487"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..2091)
                     /locus_tag="VFV20_08495"
     CDS             complement(76..2091)
                     /locus_tag="VFV20_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEU5288428.1"
                     /translation="MPRDLPLGNGDFLVTFDSRYQLRDLFYPYVGMENHTVGEPCRFG
                     VWADGSFSWVADDGWERQIAYEHETLVGDTRLRSVALGLTLRCRDAVDFDRNVYFKEV
                     TVEDESGRDRDVRLFQHFDAHLYGNEVGDSAYYDPRAQAIIHYKGRRAFLLSGASGPH
                     AFGLTSFAIGQKDAPGREGTWRDAEDGDLSRNAVAQGSIDSVAMLAAAVPAKGSTTFV
                     FWIAAGQSYDDVRELDKLVRERTPASFLARTKDYWRLWANKDETVADHVSPDIARLYK
                     RSTLVIRTQIDNRGAIIAGNDSDVLRFNRDTYSYLWPRDGALVADALDLAGYGEVTRR
                     FFYLCGELMTREGYLLHKYNPDGSLGSSWHAWSTPDGRLELPIQEDETGLPIWALWQH
                     YERDRDIEFVRPLYSKLVRAGGDFMATFREPKTGLPAASFDLWEERRGIHAFTTAAVH
                     AGLTAAHNFAVAFGQHEFAKRYAVAAQEIKEAALTHLWDGDRERFVRTVAVLPDGLIV
                     KDATVDISLAGIFLFGLLPADDPRVVSTMRAIEERLTVKTAVGGIARYENDAYFQDAQ
                     DASTVPGNPWFISTLWLAEWYIATARTMDDLERPRALLDWCLARALPSGVLPEQVHPD
                     TGAPLSVSPLTWSHAAFVSAVQHFARASARIRGGLRDQAKRAGEVIA"
     gene            complement(2095..2772)
                     /locus_tag="VFV20_08500"
     CDS             complement(2095..2772)
                     /locus_tag="VFV20_08500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEU5288429.1"
                     /translation="MAVTQRPLVLRRDLIRMFSEDRIVAVIRTATPETAETAARAMAR
                     AGVRLVEITLTVPDAYELIAKLATDDELARQGAVIGAGTVLSRAQAEDALLAGARFLV
                     SPALVPEMLEAAKARDAMSMPGTMTPTEMVKAAELGADFIKVYPIATIGGPEFITNVR
                     RALQHLPLVATGNVEYDEIPAYFKAGVVGFGIGAPLIRPDLLERGDVDGIVANAKRFL
                     AATRRSG"
     gene            complement(2774..4384)
                     /locus_tag="VFV20_08505"
     CDS             complement(2774..4384)
                     /locus_tag="VFV20_08505"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5288430.1"
                     /translation="MGSTSGRLGTPPRFERAITWSRLIGAVAVVVISPFLPTLGPIAI
                     GLLAGALVVAAVVLHVVSGRIRTPQSARRLSWITFACDVVIVSFAILLITPDPNWALV
                     PLIGVLLIITTTFRLGEIGAVLASSLMGVSLLLIAVWRSLALGLAIPTPQLAFDLLMY
                     GLTALLTTSMLREVGVLRSDVEVLRESDRERTRLLAHEREARTAAERTMTHLGAVEKI
                     SESVLRHDSVDEVMQDALDRVVTTVGASAAAILTYTPHGSVARASHGLVLRPRSTFKR
                     DTDLARALDALAQEPVTSRTLVPLSSGEEVVGILYLGFDRPRELGAEDRSLLELIATR
                     LATALERTTRLETERRARAAAESAAQRAQLLLDAADAALDTADVSERLARLARLAVPK
                     LADSSAIVLLRASGRLETVALAGVDAEKERDMWMVAHRYPRSWTALDPLFEAIRTGEP
                     RMIEHLGPDDLTALTRGSEHLRLLLEREVRSWMGIPIVVAGEVRGAIELFDGESGRVF
                     AGDDLATARLFALRLAAAVGRADGDTIG"
     gene            4504..>5034
                     /gene="pyrE"
                     /locus_tag="VFV20_08510"
     CDS             4504..>5034
                     /gene="pyrE"
                     /locus_tag="VFV20_08510"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEU5288431.1"
                     /translation="MQPGEVERIFRAAGALREGHYALASGKHSPRYLEKFQVLQWPRE
                     TERLCGAIAEAVRSFEPQTVAGPTTGGIILAHEVARQLGIRAVYAERDDKTGTGRVFR
                     RGFQLAPGERVLVVDDIMTTGGSVQETIDAVQASAGAVVAAAVLVDRSGGAAKLGVPL
                     IALWSLDIPSYAPAECP"
BASE COUNT          760 a         1751 c         1810 g          713 t
ORIGIN      
        1 ggagcgaggc gttgagaggg tatcggcggc ccgtcgccgc cgtgtgtcct cgcgtccgcc
       61 cccgccccga cgctctcacg cgatcacctc gcccgccctc ttcgcttggt cgcgaaggcc
      121 gccgcggata cgcgccgacg cgcgcgcgaa gtgctgcacg gcgctcacga atgcggcatg
      181 cgaccacgtg agcggcgaca cggagagcgg cgcgccggtg tcggggtgca cctgctcggg
      241 gagcacgcct gacggcagcg cgcgcgccag gcaccagtcg aggagcgcgc gcggccgctc
      301 caggtcgtcc atcgtgcgcg ccgtcgcgat gtaccactcg gccagccaca gcgtactgat
      361 gaaccacgga ttgccgggaa cggtgctcgc gtcctgcgcg tcctggaaat aggcgtcgtt
      421 ctcgtagcga gcgatgccgc cgacggcggt cttcaccgtg aggcgctctt cgatcgcgcg
      481 catcgtcgac acgacgcgtg gatcgtcggc gggcagcagt ccgaacagga agatgcccgc
      541 gagggagatg tccacggtcg cgtccttcac gatcaggccg tccgggagca cggcgaccgt
      601 ccggacgaag cgctcgcgat cgccgtccca caggtgcgtg agcgcggcct ccttgatctc
      661 ctgcgccgcg acggcatagc gcttcgcgaa ctcgtgctgg ccaaaggcca ccgcgaagtt
      721 gtgcgcggcc gtgagaccgg cgtgcacggc ggcggtggtg aaggcgtgga tcccgcgccg
      781 ctcctcccac aggtcgaacg acgcggccgg cagtcccgtc ttcggctcgc ggaacgtcgc
      841 catgaagtcg ccgccggcgc gcaccagctt gctgtacagc gggcgcacga actcgatgtc
      901 gcgatcgcgc tcgtagtgct gccagagcgc ccagatcggg agcccggtct cgtcctcctg
      961 gatgggcagc tcgagccggc cgtcgggcgt cgaccatgcg tgccaagacg agccgagcga
     1021 cccgtccggg ttgtacttgt ggagcaggta gccctcgcgg gtcatgagct cgccgcagag
     1081 gtagaagaaa cgccgtgtga cctcaccgta ccccgcgagg tcgagcgcgt cggcgacgag
     1141 ggcgccgtcg cggggccaga gatacgagta ggtgtcgcgg ttgaaccgca ggacgtcgga
     1201 atcgttgccc gcgatgatcg cgccgcggtt gtcgatctgt gtccggatca cgagggtgga
     1261 ccgcttgtag aggcgcgcga tgtcgggcga cacatggtcg gccaccgtct cgtccttgtt
     1321 cgcccagagg cgccagtagt ccttcgtgcg ggcgagaaag gacgccggcg tgcgctcgcg
     1381 gacgagcttg tcgagctccc ggacgtcgtc gtacgactgg ccggcggcga tccagaacac
     1441 gaacgtggtg cttcccttcg ccggcacggc ggccgccagc atcgccacgc tgtcgatgga
     1501 tccctgcgcg acggcgttcc gcgacaggtc gccgtcctcc gcgtcgcgcc aggtgccctc
     1561 ccttcccggt gcgtccttct ggccgatggc gaacgacgtc agcccgaacg cgtgcggacc
     1621 gctcgcgccc gagaggagga aggcacgccg acccttgtag tggatgatcg cctgcgcgcg
     1681 ggggtcgtag tacgccgagt cgccgacctc gttgccgtag aggtgcgcgt cgaagtgctg
     1741 gaagaggcgc acgtcacgat cgcgcccgct ctcatcctcg accgtgacct ccttgaagta
     1801 gacgttgcgg tcgaagtcca ccgcatcgcg gcagcgcagg gtgagaccga gcgcgacgct
     1861 tcgcagccgc gtgtcgccca cgagcgtctc gtgctcgtac gcgatctgcc gctcccagcc
     1921 gtcgtcggcg acccacgaga acgagccgtc ggcccacacc ccgaaacggc acggctcgcc
     1981 caccgtgtgg ttctccatgc cgacgtatgg gtagaagaga tcgcggagct ggtagcggct
     2041 gtcgaacgtg acgaggaagt cgccgttgcc gagcggcaga tcgcgcggca aggcttagcc
     2101 ggagcggcgc gttgccgcga ggaaacgctt cgcgttcgcg acgatgccgt cgacgtcgcc
     2161 gcgctcgaga aggtcgggcc ggatgagcgg cgcaccgata ccgaagccga cgacgcctgc
     2221 cttgaagtac gcggggatct cgtcgtactc gacgttgccc gtcgcgacga gtggcaggtg
     2281 ctgcagcgcg cgccgcacgt tcgtgatgaa ctcaggacca ccgatcgtcg cgatcggata
     2341 gaccttgatg aagtcggcgc cgagctcggc ggccttcacc atctccgtcg gcgtcatcgt
     2401 gccgggcatg ctcatcgcgt cgcgcgcctt cgccgcctcg agcatctccg gcacgagcgc
     2461 gggcgagacg aggaagcgcg ctcccgcgag cagtgcgtcc tcggcctgcg ctcgagacag
     2521 cacggtcccg gcgccgatga ccgctccctg gcgggcgagc tcgtcgtcgg tggcgagctt
     2581 cgcgatcagc tcgtacgcgt cggggacggt gagggtgatc tcgacgagcc ggacaccggc
     2641 tcgcgccatg gcgcgggcgg cggtctccgc cgtctccggc gtcgcggtgc ggatgaccgc
     2701 gacgatgcgg tcctcggaga acatccggat gaggtcccgc cggagcacga gtgggcgctg
     2761 ggtgacggcc atgtcaccca atcgtatcgc cgtcggcccg gcccacggcc gcggcgaggc
     2821 ggagggcgaa cagccgggcg gtcgcgagat cgtcgcccgc gaacaccctg cccgactctc
     2881 cgtcgaacag ctcgatcgcg ccccggacct cgcccgcgac gacgatcggg atgcccatcc
     2941 acgaccgcac ctcacgctcc aggagcagcc gaaggtgctc ggagccgcgg gtcagcgcgg
     3001 tcaggtcgtc cggccccagg tgctcgatca tccgcggctc gcccgtgcgg atcgcctcga
     3061 agagcggatc gagcgcggtc cagctgcgcg gatagcggtg cgcgaccatc cacatgtcgc
     3121 gctccttctc ggcatcgacg ccggcgagcg cgacggtctc gaggcgcccg ctggcacgga
     3181 gcaggacgat cgccgacgag tcggccagct tgggcacggc gaggcgcgcc aggcgcgcga
     3241 gacgctcgct cacatcggcg gtgtcgagcg cggcatcggc cgcatcgagg agcagctgcg
     3301 cccgctgcgc ggcgctctcg gccgcggcgc gggcacggcg ctcggtctcg agacgcgtcg
     3361 tacgttcgag cgccgtcgcc agacgcgtcg cgatgagctc gagcagcgag cggtcctcgg
     3421 cgccgagctc gcgcggacgg tcgaacccga ggtagaggat gccgacgacc tcctcgcccg
     3481 aggagagcgg aacgagcgtg cgcgatgtga cgggctcctg ggcgagcgcg tcgagcgcgc
     3541 gcgcgaggtc cgtgtcgcgc ttgaaggtcg accggggacg cagcaccagc ccgtgcgacg
     3601 cgcgcgccac cgagccgtgc ggtgtgtacg tcaggatcgc ggccgcggat gcgcccacgg
     3661 tcgtgaccac ccgatcgagc gcgtcctgca tcacctcgtc gaccgagtcg tggcgcagga
     3721 cgctctcgct gatcttctcg acggcaccca gatgcgtcat cgtgcgctcc gccgccgtcc
     3781 gcgcttcgcg ctcgtgcgcg agcaggcgcg tgcgctcgcg gtcggactcg cgcaagacct
     3841 cgacgtcgct tcgcaggacg cccacctccc gcagcatcga cgtcgtgagc agcgcggtga
     3901 gtccgtacat gagcaggtcg aacgcgagct gcggcgtggg gatcgcgagc ccgagcgcca
     3961 gcgagcgcca aacagcgatc aggaggaggg acacccccat gagcgacgac gcgagcaccg
     4021 cgccgatctc gccgagccgg aacgtcgtcg tgatgatgag cagcacgccg atcagcggca
     4081 cgagcgccca gttcggatcc ggtgtgatga gcaggatcgc gaaggacacg atcaccacgt
     4141 cgcaggcgaa cgtgatccac gagaggcgcc gcgcgctctg gggcgtgcgt atacgccccg
     4201 acacgacgtg gagcaccacc gctgcgacca cgagcgctcc agcgagcagc ccgatcgcga
     4261 tcggcccgag ggtggggagg aagggcgaga tgacgacgac ggcgaccgcg ccgatgaggc
     4321 gtgaccaggt gatcgcccgc tcgaaccttg ggggcgtgcc gagccgtccg gatgtgctcc
     4381 ccaccgccac ctcggatcga gtatggcctt cgccgtcggc gccgggatgg gcgcggagcg
     4441 ccccgcggga tcagggacgg atcaagcgag cgcgcgttcc tcgcgggata ctgtcgctga
     4501 gtgatgcagc caggcgaggt cgagcggatc ttccgcgccg cgggtgccct gcgcgagggg
     4561 cactacgcgc tggcctccgg taagcactcc ccgcgctatc tcgagaaatt ccaggtgctg
     4621 caatggccgc gcgagaccga gcggctctgc ggtgccatcg ctgaagccgt ccgaagcttc
     4681 gagccgcaga cggtcgccgg gcccaccacg ggtgggatca tcctcgccca cgaggtcgcg
     4741 cggcagctgg gcatccgcgc cgtctacgcc gagcgcgacg acaagaccgg caccggacgc
     4801 gtgttccgtc gcgggttcca gctggcgccg ggcgagcgag tgctcgtggt ggacgacatc
     4861 atgaccacgg gcggctcggt ccaggagacg atcgacgcgg tccaggcgtc ggccggcgcg
     4921 gtggtcgccg ctgccgtcct cgtcgaccgc tccggcggcg cggcgaagct gggcgtcccg
     4981 ctcatcgcgc tctggtcgct cgacattccc tcgtacgcgc ccgccgaatg cccg
//
LOCUS       DASTZR010000380         3430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_802733, whole genome shotgun sequence.
ACCESSION   DASTZR010000380 DASTZR010000000
VERSION     DASTZR010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802733"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="VFV20_08515"
     CDS             <1..885
                     /locus_tag="VFV20_08515"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5288432.1"
                     /translation="ALRSGAYDYLMKPSEVDELRATVNRAIERRQLRRRLLELEEVDR
                     LKTQFLSMASHELRTPLTAVSGFIQVARRRILRAADQPSDADWRHEAVRAGETLELAQ
                     RQARRLGRLIDELLDVSRLQLGRVELHQGEVDLVVALREVIERMKLLSSTHRLELDSD
                     LDAAVVTADRDRLDQVFENLIGNAVKYSPSGGDVRVVLRAVEDEVQIAITDEGIGISR
                     DELDRIFNLFYRSPDPRAGHVGGLGLGLYISREIVTRHSGRLWAESSERGSTFHVSLP
                     LKDPRSVRVKDRAGATPG"
     gene            complement(854..1111)
                     /locus_tag="VFV20_08520"
     CDS             complement(854..1111)
                     /locus_tag="VFV20_08520"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5288433.1"
                     /translation="MPARVHPDVPGEAALAKKVGQRIRALRKRKGLTMAELGADRRGR
                     VYFEKQYVWKMETGRVTPSLVALAHIARRLGTSLASLLRGL"
     gene            complement(1112..1735)
                     /locus_tag="VFV20_08525"
     CDS             complement(1112..1735)
                     /locus_tag="VFV20_08525"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5288434.1"
                     /translation="MSRTLHIASEAETLQADLYGDMVTPRVVVLVHGSGWDASGGREI
                     APRFLARGVAALALNLRGYDGSTGTSRWEESGAWSPVVDLAATKAMLRKAGAREIALV
                     GASMGGHAVLASSFDEDVECVVSISAPAQETPDELSARVRGRKLFVCANEDTLGAAPH
                     VLRAFAAADAPKTLLMFGGKEHSRGMFAAPYGEEAISAIVDFVARGE"
     gene            complement(1732..2694)
                     /locus_tag="VFV20_08530"
     CDS             complement(1732..2694)
                     /locus_tag="VFV20_08530"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEU5288435.1"
                     /translation="MADPIIVVTRPVPEKALELLRARGSVRVGPGEPPVPTADEVAHL
                     VREAEVVYTLPANPLTGDAIRGAPKLRMIATLGTGYDNIDVAAARDRGIPVTFAPGIL
                     DETTADGIFALMLAAARRLPEAERFLRAGKYRGWTPFLFLGTDVYEATLGIVGMGRIG
                     RAMARRATGFRMKILYTDAVRNEQAETEFGAAFVTLDQLLERSDFVTLHVPLMPETRH
                     LIDAKRLRQMKKTAVLINGSRGPVVDERALAEALRDRVIAAAGLDVFEREPAVEPGLL
                     ELENVVLLPHIASASERTRVRMAVRAAENILAFLDGKPLLDPVP"
     gene            complement(2746..3312)
                     /locus_tag="VFV20_08535"
     CDS             complement(2746..3312)
                     /locus_tag="VFV20_08535"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HEU5288436.1"
                     /translation="MRIREHALHLYRDDAELATTVAEFLAPAFAEAQAILTIGTRAHV
                     ASIEQRLRTEGHDVDRARWRGQYQSLDAAHVIPRLLRDGLPTAETFGEVVGGHVTRLA
                     ADHGGVRAFGEIVALLWRDGKHAAALRLEDLWNDSLGRHPLALVCGYPERDLGDEHRR
                     RIGDRHTSEIQHDAPETARRRAMPEAAS"
BASE COUNT          486 a         1233 c         1234 g          477 t
ORIGIN      
        1 gcgctgcgga gcggcgccta cgactacctc atgaagccgt cggaggtgga cgagcttcgc
       61 gccaccgtga accgtgcgat cgagcgccgg cagctgcggc gccgcctgct cgagctcgag
      121 gaggtcgatc gcctcaagac gcagttcctc tcgatggcct cgcacgagct gcgcaccccg
      181 ctgaccgcgg tcagcgggtt catccaggtg gcgcgccggc ggatcttgcg cgccgcggac
      241 cagccgagcg atgccgattg gcgccacgag gcggtccgcg ccggcgagac actcgagctg
      301 gcgcagcgcc aggcacggcg gctcgggagg ctcatcgacg agctgctcga cgtctcgcgg
      361 ctccagctcg gccgggtcga gctccatcag ggagaggtgg atctcgtcgt cgcgctgcgc
      421 gaggtgatcg agcgcatgaa gctgctgtcg tcgacgcacc gcctcgaact ggacagcgac
      481 ctcgacgccg cggtggtcac cgccgaccgt gaccggctcg accaggtctt cgagaacctc
      541 atcggcaacg cggtgaagta ctcaccgagc ggcggcgacg tgcgcgtcgt cctccgtgcg
      601 gtcgaggacg aggtccagat cgcgatcacg gacgagggca tcggcatctc gcgcgacgag
      661 ctggaccgga tcttcaacct gttctatcgg tcgcccgacc cgcgcgcggg ccacgtcggg
      721 ggcctcggac tcggcctcta catcagccgc gagatcgtca cgcggcacag cggccgcctc
      781 tgggccgaga gcagcgagcg cgggagcacg ttccacgtga gcctgccgct caaggatccg
      841 cggtccgtgc gggtcaaaga ccgcgcagga gcgacgccag gctagtcccg agccgccgcg
      901 cgatgtgcgc cagcgccacg agcgaaggcg tgacgcgacc ggtctccatc ttccacacgt
      961 actgcttctc gaagtagacg cgcccgcgcc gatcggcgcc gagctcggcc atcgtgagtc
     1021 ccttgcgctt gcggagcgcc cggatgcgct gccccacctt cttcgccagg gccgcctcgc
     1081 cgggaacgtc ggggtgcacg cgggccggca tctactctcc ccgcgcgacg aagtcgacga
     1141 tcgccgagat cgcctcctcg ccatacggcg ccgcgaacat cccacgcgaa tgctccttgc
     1201 cgccgaacat gagcagcgtc ttcggagcgt cggccgcggc gaacgcccgc agcacgtgcg
     1261 gcgccgcgcc cagcgtgtcc tcgttcgcgc agacgaacag cttgcgaccg cggacgcgcg
     1321 cggacagctc gtccggcgtc tcctgcgccg gcgccgagat ggagacgacg cactccacgt
     1381 cctcgtcgaa gctcgacgcg agcaccgcat gcccgcccat cgacgcgccg accagcgcga
     1441 tctcgcgcgc acccgccttc cgcagcatcg ccttggtggc ggcgaggtcc acgacgggcg
     1501 accaggcgcc ggactcctcc cagcggctcg tgccggtcga gccgtcgtag ccgcggaggt
     1561 tgagcgcgag cgccgcgacg ccgcgcgcga ggaagcgcgg cgcgatctcg cgcccgccgc
     1621 tcgcgtccca gccgctgccg tgcacgagca cgacgacccg cggggtgacc atgtcgccgt
     1681 agaggtcggc ctgcagcgtc tcggcttcgc tcgcgatgtg gagcgtccgg ctcacgggac
     1741 gggatcgagg agcggcttgc cgtcgaggaa cgcgaggatg ttctcggccg cgcgcacggc
     1801 catgcgcacg cgcgtccgtt cggacgcgga ggcgatgtgc ggcagcagca cgacattctc
     1861 gagctcgagg aggcccggct cgaccgcggg ctcgcgctcg aacacgtcga gaccggccgc
     1921 ggcgatgacg cgatcgcgga gcgcctcggc cagggcgcgc tcgtcgacga ccggaccacg
     1981 cgacccgttg atgaggaccg ccgtcttctt catctgccgg agacgcttgg cgtcgatgag
     2041 atggcgcgtc tcgggcatga gcgggacgtg cagcgtcacg aagtccgacc gctccagcag
     2101 ctgatccagc gtgacgaatg ccgcgccgaa ttccgtttcg gcctgctcgt tccggacggc
     2161 gtcggtgtac aggatcttca tgcggaagcc ggtggcgcgg cgcgccatgg cgcgcccgat
     2221 ccgccccatg ccgacgatgc cgagcgtggc ctcgtacacg tcggtgccaa ggaagaggaa
     2281 cggtgtccag ccgcggtact tccccgcgcg caggaagcgc tcggcctccg gcagccgtcg
     2341 cgcggcggcg agcatgagcg cgaagatgcc gtccgcggtg gtctcgtcga ggatgccggg
     2401 tgcgaacgtg accgggatgc cccggtcgcg cgccgcggcg acgtcgatgt tgtcgtaccc
     2461 cgtcccgagc gtggcgatca tgcgcagctt cggcgcgcca cggatggcgt caccggtgag
     2521 ggggttcgcc ggcagcgtgt acacgacctc cgcctcgcgg acgaggtgcg cgacctcgtc
     2581 ggcggtcggc acgggcggct cgccggggcc gacgcgcacg ctcccacgcg cacggagcag
     2641 ctcgagggcc ttctcgggaa cgggccgggt gacgacgatg atcgggtcag ccacgcgtcg
     2701 tgagcatacg tcggggacgc cgcgcgtgag gcgccctcgc cgcgatcagc tcgcggcttc
     2761 gggcatcgcg cgccggcgcg ccgtctccgg agcgtcgtgc tggatctcgc tggtgtgacg
     2821 gtcgccgatg cgacggcgat gctcgtcgcc gaggtcgcgc tcgggatagc cgcacaccag
     2881 ggcgagcggg tgacggccga gggagtcgtt ccacaggtcc tcgaggcgca gcgcggcggc
     2941 gtgctttccg tcgcgccaca ggagcgcgac gatctcgcca aaggcgcgca cgccgccgtg
     3001 gtcggcggcc agacgcgtga cgtgccctcc gacgacctcg ccgaacgtct ccgctgtcgg
     3061 taggccgtcg cgcagcagcc gtgggatgac gtgcgccgcg tcgaggctct ggtactgccc
     3121 gcgccagcgc gcgcgatcca cgtcgtgacc ttcggtgcgc aggcgctgtt cgatggaggc
     3181 gacgtgtgcc cgggtgccga tcgtgaggat ggcctgcgcc tccgcgaacg cgggcgcgag
     3241 gaattcggcg acggtcgtcg ccagctccgc gtcgtcccgg tacaggtgga gcgcgtgttc
     3301 cctgatcctc atggcgggcc gaccccctgc cgcaagtgcc atgccagcga gcaagcaacg
     3361 gctgccgtcg atatcgtccg gtggtgccac ccgaccgccg cagcgcacgc cgagcccctc
     3421 ccgcgcgccg
//
LOCUS       DASTZR010000381         2136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_329379, whole genome shotgun sequence.
ACCESSION   DASTZR010000381 DASTZR010000000
VERSION     DASTZR010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329379"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /gene="hisH"
                     /locus_tag="VFV20_08540"
     CDS             <1..433
                     /gene="hisH"
                     /locus_tag="VFV20_08540"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEU5288437.1"
                     /translation="SRGLDRPMREVRERRIPLLGLCVGMQLFYESSEEDAGAKGLGFI
                     AGTVRRLRGAVRVPHTGWNAVHLRRAHPWLADVADGSHFYFVHSYAPDAAGEAVLATT
                     DHGADFASIVGGDRFLGVQFHPEKSSLMGTRLLHGFVRGIS"
     gene            433..1209
                     /gene="hisF"
                     /locus_tag="VFV20_08545"
     CDS             433..1209
                     /gene="hisF"
                     /locus_tag="VFV20_08545"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEU5288438.1"
                     /translation="MLAKRIVPCLDVKEGRVVKGTQFVELRDAGDPVTLARRYDREGA
                     DELVFLDITASHERRKTVIELAKRVAEVLTIPFCVGGGIASADVAAAILRAGADKVAV
                     NTAAVHRPELLGEIASRAGAQAAVLAIDARRRADGGYEVYVEGGRTPTGLDAIEWARR
                     GVAAGAGEILLTSMDRDGTRDGFELALTARIAEAVNVPVIASGGAGTTDHFVELFART
                     GADAGLAASIFHSGEIVVADLKRALAERGVAVRPSAEAGT"
     gene            1206..1898
                     /gene="hisIE"
                     /locus_tag="VFV20_08550"
     CDS             1206..1898
                     /gene="hisIE"
                     /locus_tag="VFV20_08550"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008880304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HEU5288439.1"
                     /translation="MSEDEPTNATGSARRADPGHAEEVAFDDRGLVPAIVQDAATGAV
                     LMLAYMDREALEATLRTREVHFHSRSRGRLWKKGETSGNVLHLVDITADCDADALLVK
                     AHPAGPACHTGDATCFGPVNERDLGRFLSELAAMLAQRKRDLPAGSYSADLFRAGPDA
                     IAAKLVEEAGETAAALRGEGRARTISELTDLLYVMLVLATDVGATPDEIRASLVEKRA
                     AAPGRRAQREGH"
     gene            complement(1831..>2136)
                     /locus_tag="VFV20_08555"
     CDS             complement(1831..>2136)
                     /locus_tag="VFV20_08555"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEU5288440.1"
                     /translation="GSFAAIVISVEAGVGKPDPRIFRLAADRLAVAARDCVYVGDSLV
                     RDVAGAHAAGMRAVWLDRGLWQEPEHIRPDARIERLSDLPAALDALARRPASPPATP"
BASE COUNT          302 a          762 c          795 g          277 t
ORIGIN      
        1 gtcgcgcggg ctcgaccgtc cgatgcgcga ggtccgcgag cgccgtatcc cgctcctcgg
       61 cctctgcgtc gggatgcagc tcttctacga atcgagcgag gaggacgcgg gcgcgaaggg
      121 cctcgggttc atcgccggca cggtgcggcg cctgcgcggc gcggtgcgcg tgccgcacac
      181 cggatggaac gccgtgcact tgcgacgagc gcacccctgg ctcgcggacg tcgccgacgg
      241 cagccacttc tacttcgtgc actcctacgc gccggatgcg gcgggcgagg ccgtcctcgc
      301 gacgaccgat cacggcgcgg acttcgcgtc gatcgtcggt ggcgacaggt tcttgggcgt
      361 ccagttccac cccgagaaga gctcgctcat ggggacgcgg ctcctgcacg gcttcgtgcg
      421 ggggatctcc tgatgctcgc caaacgcatc gtcccctgcc tcgacgtgaa ggaaggccgc
      481 gtcgtgaaag gcacgcagtt cgtcgagctg cgcgacgcgg gtgatccggt cacgctcgcg
      541 cgccgctacg accgcgaggg cgcggacgag ctcgtgttcc tcgacatcac cgcgtcacac
      601 gagcgccgga agaccgtgat cgagcttgcg aagcgggtgg ccgaggtgct gaccatcccg
      661 ttctgcgtgg gcggtggcat cgcgtccgcg gatgtggccg cggcgatcct ccgcgcgggc
      721 gcggacaagg tcgcggtgaa cacggcggcg gtccaccggc ccgagctcct gggcgagatc
      781 gcgtcgcgcg cgggcgcgca ggcggccgtg ctcgcgatcg acgcgagacg ccgggccgac
      841 ggtggctacg aggtctacgt cgagggcggg cggacgccga cgggcctcga cgcgatcgaa
      901 tgggcgcgcc gtggcgtggc ggcgggcgcc ggcgagatac tcctcacctc gatggaccgc
      961 gacggcacgc gcgacggctt cgagctcgca ctgaccgcgc gcatcgccga ggcggtgaac
     1021 gtgccggtga tcgcgtctgg cggcgcgggc acgaccgacc acttcgtcga gctcttcgcg
     1081 aggaccggcg cggacgcggg actcgcggcg tcgatcttcc actccggcga gatcgtcgtc
     1141 gcggatctca agcgggcgct ggcggagcgc ggcgtcgcgg tgcgcccgag cgcggaggcg
     1201 ggcacgtgag cgaagacgag ccgacgaacg cgacgggcag cgcgcggcgt gccgatcccg
     1261 gacacgcgga ggaagtcgcg ttcgacgacc gcggcctcgt cccggcgatc gtgcaggacg
     1321 ccgcgaccgg agcggtgctc atgctcgcgt acatggaccg cgaggcgctc gaggcgacgc
     1381 tgcgcacgcg cgaggtccat ttccacagcc gctcgcgcgg ccggctctgg aagaagggcg
     1441 agaccagcgg caacgtgctc cacctcgtcg acatcaccgc cgactgcgac gcggacgcgc
     1501 tgctcgtgaa agcgcatccg gccgggcccg cctgccacac cggcgacgcc acgtgcttcg
     1561 ggccggtgaa cgagcgcgac ctcggccggt tcctctcgga gctcgccgca atgctggccc
     1621 agcgcaaacg cgaccttccc gccggctcct actcggccga tctctttcgc gccggaccgg
     1681 acgcgatcgc ggcgaagctc gtcgaggagg cgggcgagac cgcggccgcg ctgcgcggcg
     1741 agggccgcgc gcgcacgatc agtgagctga ccgacctgct ctacgtgatg ctcgtcctcg
     1801 cgaccgatgt cggcgccacg cccgacgaga tcagggcgtc gctggtggag aagcgggccg
     1861 ccgcgccagg gcgtcgagcg cagcgggaag gtcactgagc cgctcgatcc gcgcgtccgg
     1921 ccggatgtgc tccggctcct gccacaggcc gcgatcgagc cacaccgcgc gcatgcccgc
     1981 ggcgtgcgcc ccggcgacgt cgcgcacgag cgaatcgccg acgtagacgc agtccctcgc
     2041 cgcgaccgcg agccgatcgg ccgccagcct gaagatgcgc gggtcgggtt tccccacgcc
     2101 cgcctcgacc gagatgacga tggcggcgaa ggagcc
//
LOCUS       DASTZR010000382         3061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1170113, whole genome shotgun sequence.
ACCESSION   DASTZR010000382 DASTZR010000000
VERSION     DASTZR010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170113"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1802)
                     /locus_tag="VFV20_08560"
     CDS             complement(<1..1802)
                     /locus_tag="VFV20_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5288441.1"
                     /translation="MSRRDPWSFIGLLAFFILLFVALFGERIAPHEAIYFVPEHGRDP
                     RPYDPGLVYPFGSDVLGRDLVSLVLAGARLTLGIVVLGGLARVLAALLLAVIASLSAR
                     ARAALDATSEIVAAVPATLVVLLVVLVFVHGDTTALVFVGALLVTGWAGPYRVLRAEL
                     DRLARVAFTEGAAAVGAGRLRIVLRHHVPHVVPLLAMNASQQVVASLVALAELGVLGV
                     FVGTTRLVNIEESLSVVRSGERNFAFIADPPEWGGLLANARDTESLWTTRWVFLIPGL
                     AFAVAAVAVAAIGVAVARRYARRDVVAELRGRGARVLLFGVLALVVAANVVPERYAEA
                     RSWAADARASVRGSTGDAAAVFADAGLAPIGPGYTVERDVAAIRATGPATVQVGAATV
                     RESSTGPLDVLPLLYADSGGGTLNAPLVYASWGLSPADFRTPPPSIFDPPAIGKTIAD
                     WPDDYANVDVRGKVVVVLRVMGIVTGTRQMSGPDPETSIANALRRGAAGVLFVDPALP
                     QLPRVQTSRSTNLYLRLMANSPIQTVVGRPVIVLSLSAADRLLAPAGIAPSEDYAALE
                     SRMIPTDGERATRSVARALDMNARIDVPTELSRA"
     gene            complement(1879..1968)
                     /locus_tag="VFV20_08565"
     tRNA            complement(1879..1968)
                     /locus_tag="VFV20_08565"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1932..1934),aa:Ser,seq:gct)
     gene            2036..>3061
                     /locus_tag="VFV20_08570"
     CDS             2036..>3061
                     /locus_tag="VFV20_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1786 domain-containing protein"
                     /protein_id="HEU5288442.1"
                     /translation="MPDARILAIDIGAGTMDVLLHDPAQPMENAIQLVLPSATAIVGR
                     RIESARRAGRPVFLHGNLMGGYHTSNAVWRHLEAGLAVYATERAARTVHDDIDLLARR
                     GVRITDAAPDDAVAIEMHDLDLARLAKVLGPYDVTLPATVAVAAQDHGFSPKASNRLF
                     RFEHWKRFLVPGGTLADHIWTQPPEYMTRLLAIQQDAPGAIVADTGPVAVLGALEDEH
                     VAREAERGACIVNVGNQHTLGLLVRGEELFGVMEHHTESMTTEKLERLISRLVAGTIT
                     HDEVFADDGHGALILDAYREVGPFPFVAVTGPNRAMARPLGWYFAAPHGAMMLSGCFG
                     LVRGVKEK"
BASE COUNT          505 a         1110 c         1065 g          381 t
ORIGIN      
        1 tgcgcgcgag acagctcggt cggcacatcg atgcgcgcat tcatatcgag cgcacgcgcg
       61 acggagcggg tggcacgctc cccgtcggtc gggatcatgc gcgactccag cgcggcgtag
      121 tcctccgacg gtgcgatgcc cgccggagcc agcagccggt ccgcggcgga caggctgagc
      181 acgatgaccg gccgcccgac gaccgtctgt atcggcgagt tcgccatgag tcgcagatac
      241 aggttcgtgc tccggctcgt ctggacgcgt ggcagctgcg gcagcgcggg gtccacgaac
      301 agcacgccgg cggcgccgcg cctcagcgcg ttggcgatgc tcgtctccgg gtccggcccg
      361 ctcatctgcc gggtgccggt gacgatgccc atcacccgga gcaccaccac caccttgccc
      421 cgtacatcca cgttcgcgta atcgtcgggc cagtccgcga tcgtcttgcc gatggcgggc
      481 gggtcgaaga tcgagggtgg cggcgtgcga aagtccgccg gcgacagtcc ccagctggcg
      541 tacacgagcg gcgcgttcag cgtgcctccg ccactgtcgg cgtacaggag cgggagcacg
      601 tcgagcggtc ccgtggacga ttcccgcacg gtcgccgcgc cgacctggac cgtcgccggc
      661 cccgtggcgc ggatcgccgc cacgtcgcgc tcgacggtgt atcccggacc gatgggagcg
      721 agccccgcgt cggcgaacac cgcggcggcg tcccccgtcg atccgcgcac cgaggcccgc
      781 gcatccgccg cccacgagcg cgcctccgcg tagcgttcgg gcacgacgtt cgccgcgacg
      841 accagcgcca ggacgccgaa gagcagcacc ctcgcgccgc ggccgcgtag ctcggcgacg
      901 acgtcgcgac gcgcgtagcg gcgcgcgacg gccacgccga tcgcggcgac cgcgaccgcc
      961 gccaccgcga acgcgagacc ggggatgagg aagacccaac gcgtcgtcca aaggctctcg
     1021 gtgtcgcgcg cgttcgcgag gaggccgccc cattccggag gatcggcgat gaacgcgaaa
     1081 ttgcgctcgc cgctgcgcac cacgctcagc gactcctcga tgttcaccag cctcgtggtg
     1141 ccgacgaaca cgccgaggac accgagctcg gccagcgcga cgagcgacgc gacgacctgc
     1201 tggctggcgt tcatcgcaag tagcggcacc acgtggggga cgtggtgccg gagcacgatg
     1261 cgcagacgcc cggcgccgac cgccgccgct ccctcggtga acgccacgcg agcgagccgg
     1321 tcgagctcgg cgcggagcac gcgatacggg ccggcccatc ccgtgacgag cagcgcaccg
     1381 acgaacacca gcgcggtcgt gtccccgtgc acgaacacga gcacgaccag gaggacgacg
     1441 agggtggccg gtaccgccgc gacgatctcg gacgtggcat cgagcgcggc gcgggcacgc
     1501 gccgagagcg acgcgatgac cgcgagcagc aacgccgcga ggacgcgtgc gaggccgccg
     1561 agcacgacga tgcccagcgt gagtcgcgcg ccggcgagga cgaggctcac gagatcccga
     1621 ccgagcacgt ccgacccgaa cgggtagacg agcccgggat cgtacggacg aggatcgcgg
     1681 ccgtgctctg ggacgaaata gatcgcctcg tgcggcgcga tgcgttcacc gaagagcgcg
     1741 acgaagagga ggatgaagaa cgcgaggaga ccgatgaatg accaggggtc tcgccgcgac
     1801 atcaccggaa gataccggcg atgacgtcag tcagttcggg acatgccggt gaatgacccg
     1861 cgccgggaat tggatctgcg gggagggcgg gattcgaacc cgcggagcca gaaaacccga
     1921 ctcagaggtt tagcaaacct ccgcactcga ccactatgcg acctcccccg aggtccctgg
     1981 tgatcaccag tctaccgttt gaaggccacc acggcacgct cgctagcatc gaggcatgcc
     2041 cgacgcgcgc atcctcgcga tcgacatcgg cgcgggcacg atggacgtgc tcctccacga
     2101 cccggcacag ccgatggaga acgcgatcca gctcgtcctg ccgtccgcca ccgcgatcgt
     2161 cggccgccgc atcgagagcg cgcgccgcgc cggccggccc gtgttcctgc acggcaatct
     2221 catgggcggc taccacacct cgaacgcggt gtggcggcat ctcgaagcgg gcctcgcggt
     2281 gtacgcgacc gagcgcgccg cgcgcaccgt gcacgacgac atcgacctcc tcgcccggcg
     2341 cggggtccgc atcaccgacg cggcgccgga tgacgccgtc gcgatcgaga tgcacgacct
     2401 cgacctcgcg cgcctcgcga aggtgctcgg gccgtacgac gtgacgctgc ccgccacggt
     2461 ggccgtcgcg gcgcaggacc acggcttctc gccgaaggcg tcgaaccgcc tgttccgctt
     2521 cgagcactgg aagcgcttcc tcgtgccggg cggcacgctc gccgatcaca tctggaccca
     2581 gcccccggag tacatgacgc gcctgctcgc gatccagcag gacgcgcccg gtgcgatcgt
     2641 cgcggatacc ggtccggtcg cggtgctcgg cgcgctcgag gacgagcacg tcgcccgcga
     2701 ggccgagcgc ggcgcgtgca tcgtgaacgt cggcaaccaa cacacgctcg gtctgctcgt
     2761 gcgcggcgag gagctgttcg gcgtgatgga gcaccacacc gagtcgatga ccaccgagaa
     2821 gctcgagcgc ctcatctccc gcctcgtcgc gggcacgatc acgcatgacg aggtgttcgc
     2881 cgacgacggc cacggcgccc tcatcctcga cgcctaccgc gaggtcgggc cgttcccctt
     2941 cgtcgccgtc accgggccga accgcgcgat ggcgaggccg ctcggctggt acttcgcggc
     3001 gccgcacggc gcgatgatgc tcagcggctg cttcggactc gtgcgcgggg tgaaagagaa
     3061 g
//
LOCUS       DASTZR010000383         3064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_256483, whole genome shotgun sequence.
ACCESSION   DASTZR010000383 DASTZR010000000
VERSION     DASTZR010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256483"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..2099
                     /locus_tag="VFV20_08575"
     CDS             96..2099
                     /locus_tag="VFV20_08575"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEU5288443.1"
                     /translation="MTWADLGSAALRAALPVAIWGCGAAAYAAAKRDGRGLASARWSS
                     LLAFVLVSLAIVGMEGSLLTHDFSVQYVAQNNARATPTFFTVISLWAALEGSILLWTL
                     VLAGATAYVAWRGTVALPRLATAALSVLLAMLAFFLLLVTTPAADPFVRIEPPLDGNG
                     PNPLLQNHPLMALHPPLLYLGYVLFSVPFAYAIASLVLGEGGDRWLVATRRFALVSWA
                     LLGIGIVAGSWWSYAVLGWGGYWAWDPVENAAIMPWLTATAYLHSVMVEEKRRLLRTW
                     NLSLVITTFALTILGTVLTRSGVVNSVHAFTQSAIGPLLLGYFVAIVVLSVGLLLWRM
                     PRLVDAGGIGAPLSREAIFLFQNVVFVAATMTVLLGTLYPLIAEAISGAQLSIGRPYF
                     DRVEVPLALALLFLMGIGPQLPWHGASRATLERQFAIPIVAAAAGAILAVLIGMSGVF
                     VVLTYALAAFVAATVAQEFARGVRARAVLHGEAPPLAFANLVRRSGRRYGGYVVHLGI
                     VVIAVAVATSQSGMIQVEKTLAPGDSVAVGPYVVRLDALREVSEPQRDLLVADVTVTG
                     NGAADRLRPALVFYPNASQPIGSPGIAAGLRDDVYTILAAYDARAKAWATIRVHIMPL
                     VSWLWIGGAIVLLGAVIAALPQPRRSPAGRRAAETPVAVEGQV"
     gene            2100..2666
                     /locus_tag="VFV20_08580"
     CDS             2100..2666
                     /locus_tag="VFV20_08580"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU5288444.1"
                     /translation="MPRWLRLAVVVGIGALVLVLVLAFRRAPQDIRTGTVGKPAPAFT
                     LQRLDGGGEYTVTPGDGKVFVLNFFASWCIPCKQENPSLLRVYERYRGSGVEFVGILY
                     QDSAEAGLRYIRDNGNTWPSVNDDRGAVAFAYGVFGIPETYFIGADGIIAGRHIGPID
                     DATLTGGIECLRSTTVKAEDCLRASRKR"
     gene            2663..>3064
                     /locus_tag="VFV20_08585"
     CDS             2663..>3064
                     /locus_tag="VFV20_08585"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HEU5288445.1"
                     /translation="MTSRGALLRPRTLIVLAILTLALALAWSARPHTETSAERVDRIT
                     AELRCVVCQGLSVKDSPAESARQMRDRVVQRVAEGRSDQEIVDEFRASYGDWVVLSPP
                     LLSWSALVWLVPIVAVLVGTAVAWRWLRAPPR"
BASE COUNT          354 a         1114 c         1084 g          512 t
ORIGIN      
        1 gaggacggga cgttccgcgc cacgaccgtg atcgccaaac acgacgagaa gtacgcgccg
       61 ccctcgcccg gcaagacgcc ggcgcacccc accccgtgac ctgggccgat ctcggcagcg
      121 ccgcgctgcg cgccgcgttg ccggtcgcca tctggggctg cggcgcggcg gcgtacgcgg
      181 cggccaagcg cgacgggcgg ggactcgcgt cggcgcgctg gtcgtcgctg ctcgcgttcg
      241 tgctcgtctc gctcgcgatc gtgggcatgg aggggtcgct gctcacgcac gacttctccg
      301 tgcagtacgt cgcgcagaac aacgcgcgcg ccacgccgac cttcttcacg gtgatctcgc
      361 tctgggccgc gctcgaaggc tccatcctgc tgtggacgct cgtgctcgcg ggcgcgaccg
      421 cgtacgtcgc gtggcgcggc acggtcgcac tgccgcggct cgcgacggcc gcgctctcgg
      481 tgctgctcgc aatgctcgcg ttcttcctgc tgctggtgac gacgccggcg gccgatccgt
      541 tcgttcgcat cgagccgccg ctcgacggca acgggccgaa cccgctgctc cagaaccacc
      601 cgctgatggc actgcacccg ccgctcctct acctcgggta cgtgctcttc tcggtgccct
      661 tcgcgtacgc gatcgcgtcg ctcgtcctcg gcgagggcgg cgatcgctgg ctcgtggcaa
      721 cgcgccgctt cgcgctcgtg tcgtgggcgc tcctcggcat cgggatcgtc gcgggatcgt
      781 ggtggtcgta cgcggtcctc gggtggggcg ggtactgggc atgggatccc gtggagaacg
      841 ccgcgatcat gccgtggctc accgcgaccg cgtacctgca ctcggtgatg gtggaggaga
      901 agcggcgcct cctgcgcacc tggaacctct cgctggtcat cacgacgttc gcgctcacga
      961 tcctcggcac ggtcctcaca cgcagcggcg tggtgaactc ggtgcacgcg ttcacgcagt
     1021 cggcgatcgg cccgctgctc ctcgggtatt tcgtcgcgat cgtggtcctg tcggtgggcc
     1081 tgctgctctg gcgcatgccg cgcctcgtcg acgcgggcgg catcggcgcg ccgctctcgc
     1141 gcgaggcgat cttcctgttc cagaacgtcg tcttcgtcgc ggcgaccatg acggtcctgc
     1201 tcggcacgct ctacccgctc atcgccgaag cgatctccgg cgcgcagctc tccatcggcc
     1261 gtccgtactt cgatcgggtc gaggtgccgc tcgcgctcgc gctgctgttc ctcatgggca
     1321 tcggaccgca gctgccgtgg cacggtgcct ctcgcgcgac gctcgagcgc cagttcgcga
     1381 tccccatcgt ggccgcggcg gctggggcga tcctcgcggt gctcatcggc atgagcggcg
     1441 tcttcgtcgt cctcacctac gccctcgcgg cgttcgtcgc ggcgaccgtc gcgcaggagt
     1501 tcgcacgcgg cgtgcgcgcg cgggccgtcc tccacggcga agcgccgccg ctcgccttcg
     1561 cgaacctggt gcggcggagc ggcaggcgct acggcggcta cgtcgtgcac ctcggcatcg
     1621 tggtgatcgc ggtcgcggtc gcgacgagcc agtccgggat gatccaggtg gagaagacgc
     1681 tcgcgccggg cgacagcgtc gcggtcggcc cgtacgtggt ccgcctcgac gcgctgcgcg
     1741 aggtctccga gccgcagcgc gacctgctcg tcgcggacgt cacggtgacg gggaacggcg
     1801 ccgctgatcg ccttcgcccc gcgctcgtct tctatccgaa cgcgtcgcag ccgatcggct
     1861 cgccgggcat cgcggcgggc cttcgtgacg acgtgtacac gatcctcgcg gcgtacgacg
     1921 cccgcgcgaa ggcgtgggcc acgatccgcg tgcacatcat gccgctcgtg tcgtggctct
     1981 ggatcggcgg cgcgatcgtc ctcctgggcg cggtcatcgc cgcgctgccg cagccgcggc
     2041 gctcgcccgc gggccggcgc gcggccgaaa cgcccgtcgc cgtggagggg caggtctagg
     2101 tgcctcgctg gctccggctc gccgtcgtcg tcgggatcgg cgcgctcgtg ctcgtcctcg
     2161 tgctcgcgtt ccgtcgggcg ccgcaggaca tccggacggg cacggtgggc aagcccgcgc
     2221 cggcgttcac gctgcagcgg ctggacggcg gcggcgagta caccgtgacg cccggcgacg
     2281 ggaaggtctt cgtcctgaac ttcttcgctt cgtggtgcat cccgtgcaag caggagaacc
     2341 cgtcgctcct ccgcgtgtac gagcgctatc gcgggagcgg tgtcgagttc gtcggcatcc
     2401 tctatcaaga ctccgccgag gcgggcctca ggtacatccg tgacaacggc aacacctggc
     2461 ccagcgtgaa cgacgaccgc ggcgcggtcg ccttcgccta tggcgtattc gggatccccg
     2521 agacgtactt catcggtgcg gacgggatca tcgccggccg gcacatcggt ccgatcgacg
     2581 acgcgacgct cacgggcggc atcgagtgcc tgcgctcgac gacggtgaag gccgaagact
     2641 gcctgcgcgc gagcaggaag agatgacgtc gcgcggcgcg ctgctccggc cgcgcacgtt
     2701 gatcgtcctc gccatcctca cgctcgcgct cgcgctcgcg tggagcgcgc ggccgcacac
     2761 cgagacctcg gcggagcgcg tcgatcgcat caccgccgag ctgcgctgcg tcgtgtgcca
     2821 gggcctctcg gtgaaggact cccccgccga gagcgcgcgc cagatgcgcg accgcgtcgt
     2881 gcagcgcgtg gccgagggtc gcagcgacca ggagatcgtc gacgagttcc gcgcgtccta
     2941 cggggattgg gtcgtcctgt caccgccgct gctctcatgg tccgcgctgg tgtggctcgt
     3001 gccgatcgtc gccgtgctcg tcggcactgc ggtcgcgtgg cgctggctgc gcgcgccgcc
     3061 gcgg
//
LOCUS       DASTZR010000384         3621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_98848, whole genome shotgun sequence.
ACCESSION   DASTZR010000384 DASTZR010000000
VERSION     DASTZR010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98848"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..676)
                     /locus_tag="VFV20_08590"
     CDS             complement(23..676)
                     /locus_tag="VFV20_08590"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5288446.1"
                     /translation="MLENFTWYKQSAFRFKGDRLVMYIDPWGLEGDLPPADIVLITHA
                     HGDHYSNDDLKKVKAAKTVFVAPADVAKELSGNVKVVRPGERVDAAGLKLETVPAYNI
                     DPQRLQAHPKANNWVGYLMPLGGHTYYHAGDTDHLPELERIKTDVAFVPIGDGGFVMT
                     VDEAAALVKAMKPKLAVPMHYGFYPGVGVEADGPRFKQAASGVEVQILKPVNAFANN"
     gene            complement(677..1576)
                     /locus_tag="VFV20_08595"
     CDS             complement(677..1576)
                     /locus_tag="VFV20_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU5288447.1"
                     /translation="MRLATFDPGTGPLASVVADDDTAIAASDLVPGAPDEMVELIAAG
                     EQLHSRLRAAAASARGGVPLRSGRLLAPIPRPKRDVFCVGWNYSEHFAEGKNFRLAQG
                     TPGAQEIPEFPALFSKNPATVTGPDMPIWFPAPHSTELDWEVELAVIIGTSGRDITEE
                     RAMSHVFGYTCGNDVSVRDVQRRHGGQWFKGKNFDSHMPLGPWIVTADAIDPAALGVR
                     TRVNGVTKQDSNTRFMVFKIPRLIREFSAGCDLVPGDIIITGTPEGVGFARKPPEFMK
                     IGDVVEVEIEGIGVLRNTVQGRT"
     gene            complement(1576..2085)
                     /locus_tag="VFV20_08600"
     CDS             complement(1576..2085)
                     /locus_tag="VFV20_08600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEU5288448.1"
                     /translation="MPVDRDRYRSLAGSFPTGVTIITARAADGVPRGLTTQSFVGLST
                     EPPLMLVSIDKTSRTLVALRGARGFVINFLKIGAEEVSTVFASKSEDKFSGVPWRPSD
                     VADGAPILIEAAHAYAECVTERSFEAGDHVIVIGRVEGGEVLGGTPLLYYRRTYAGWP
                     EERPAPQVG"
     gene            2152..2889
                     /locus_tag="VFV20_08605"
     CDS             2152..2889
                     /locus_tag="VFV20_08605"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEU5288449.1"
                     /translation="MPRQTTRAQRRREARRAEAARPAKGSRRGGGISRGWVIGASVVA
                     VAAVAVLIWASNASSTTPPVTTVPPGITQSGQTRGDPAAPVTIDEWADFQCPACGVFA
                     RQTEPQIVSTYVAKGQVKIAFHNFAFLGPESNWAAEAALCAGEQGKFFEFHDRLFASQ
                     AGENNGAFAKDKLKKMGTDLGLSPSFAACVDSGKYAQSVRDEQNVGQGKDVSRTPTLF
                     VNGKKYEGAYSFDEMKKIIEPLLAGGR"
     gene            2889..3302
                     /locus_tag="VFV20_08610"
     CDS             2889..3302
                     /locus_tag="VFV20_08610"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEU5288450.1"
                     /translation="MLGRRAALVLATIGTAVSAYLTWIHYSGAFAFCAGVGDCEVVQT
                     SRYSMLGPIPVALIGLVGMVLILGVSLGRLMSDDPRLDLALFAFALMATAYVAYLSYV
                     EVFVLGAICPWCVAVAVCSVAIFALAARDVMSASS"
     gene            complement(3283..>3621)
                     /locus_tag="VFV20_08615"
     CDS             complement(3283..>3621)
                     /locus_tag="VFV20_08615"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEU5288451.1"
                     /translation="ARSAVVVAAVAAGVLAFDGIWADFRDEKGLRDDAMRGRRLGFSG
                     RQCIHPDQIAVVNEIFSPAPDEVAHARRVVAAYEDGVAKGLGAVALDGEMLDPPIVER
                     ARRVLRMTRS"
BASE COUNT          550 a         1264 c         1218 g          589 t
ORIGIN      
        1 cctcggtttg ttgcagtcga cgctaattgt tggcgaacgc attcaccggt ttcaggatct
       61 ggacctcgac gcccgacgcc gcctgcttga agcgcggccc gtcggcctcg acgccgacgc
      121 cggggtagaa cccatagtgc atcggcacgg ccagcttcgg cttcatcgcc ttcaccagcg
      181 cggccgcctc gtcgacggtc atgacgaagc caccgtcgcc gatcggaacg aacgcgacat
      241 cggtcttgat acgctcgagc tcgggcaggt ggtcggtgtc gccggcgtgg tagtaggtgt
      301 ggccgccgag cggcatgaga tagccgaccc agttgttcgc cttgggatgc gcctgcagcc
      361 gctgcgggtc gatgttgtac gcaggcaccg tctcgagctt caggccggcc gcgtcgacgc
      421 gctcgcccgg tcgcacgacc ttcacgttgc cggagagctc tttcgcgaca tccgcgggag
      481 cgacgaatac cgtcttcgcc gccttcacct tcttcaggtc gtcgttcgag tagtgatcgc
      541 cgtgcgcgtg cgtgatgagc acgatgtcgg ccggcggtag atcgccctcg agaccccagg
      601 ggtcgatgta catcacaagc cggtcgccct tgaagcgaaa tgccgactgc ttgtaccagg
      661 tgaagttctc gagcacttag gtcctcccct gcaccgtatt ccgtaggacg ccgatgcctt
      721 cgatctcgac ctcgaccacg tcgccgatct tcatgaactc aggtggcttc cgcgcgaatc
      781 caacgccctc tggagtgccg gtgatgatga tgtcgccggg caccaggtcg caccccgccg
      841 agaactcgcg aatgaggcgc gggatcttga acaccatgaa tcgggtattc gaatcctgtt
      901 tcgtcacgcc gttcacacgc gtacgcacgc ccagcgccgc ggggtcgatc gcgtcggcgg
      961 tgacgatcca cggtccgagg ggcatgtggc tgtcgaagtt ctttcccttg aaccactgac
     1021 cgccgtgacg ccgctgcacg tcgcggaccg agacgtcgtt gccgcaggtg tagccgaaca
     1081 cgtgcgacat cgcgcgttcc tcggtgatat cgcggccgga cgtgccgatg atgaccgcga
     1141 gctcgacctc ccagtcgagc tccgtcgagt gcggcgcggg gaaccagatc ggcatatcgg
     1201 gcccggtcac ggtggcgggg ttcttcgaga agagcgcggg gaactccggg atctcctgcg
     1261 cgccgggtgt cccctgcgcg agacggaagt tcttcccctc ggcgaagtgc tcggagtagt
     1321 tccatccgac gcagaagacg tcgcgcttgg gccgcgggat gggagcgagg aggcgcccgg
     1381 agcgaagagg cacgccaccg cgggcggacg ccgcggccgc tcgcagacgc gagtgcagct
     1441 gctcgccggc agcgatgagc tccaccatct catcgggtgc gccggggacg agatcgctcg
     1501 ccgcgatggc cgtgtcgtcg tccgcgacga cgctcgcgag cgggccggtg ccggggtcga
     1561 acgtggcgag gcgcatcagc cgacctgcgg cgccggccgc tcctcgggcc agcccgcgta
     1621 cgtgcgccgg tagtacagca gcggcgtgcc gccgagcacc tcgccgccct cgacgcgccc
     1681 gatgacgatc acgtgatcgc cggcctcgaa gctccgctcg gtcacgcatt cggcgtaggc
     1741 atgcgcggcc tcgatgagga tcggcgctcc gtcggccacg tcggacggac gccacggtac
     1801 gccgctgaac ttgtcctctg acttggatgc gaacacggtc gagacctcct cagcgccgat
     1861 cttcaggaaa ttgatgacga agccacgcgc gccgcgcagc gccacgagcg tgcgcgacgt
     1921 cttgtcgatc gagacgagca tgagcggcgg ctccgtgctg aggccgacga acgattgcgt
     1981 cgtgagcccg cgcggaacgc cgtcggcggc gcgtgcggtg atgatcgtca cgcccgtcgg
     2041 gaacgagccg gccaggctcc ggtagcggtc gcggtccacc ggcatgggtt tccgagtcta
     2101 gggacattcg gccctagcgc cgcccgggca gtgcgtccgt accatcgggt gatgccccgc
     2161 cagaccaccc gtgcgcagcg gcgccgcgag gcgcgcaggg ccgaggccgc gcgtcccgcc
     2221 aaagggagcc gccgtggcgg cggcatctcg cgcggctggg tcatcggcgc gtcggttgtc
     2281 gccgtcgctg cggtggcggt gctcatctgg gcgagcaacg cgagcagcac gacaccgccc
     2341 gtcaccaccg taccgccggg gatcacgcag agcggccaga cccgcggcga tcccgccgca
     2401 cccgtgacga tcgacgagtg ggccgacttc cagtgcccgg cctgcggcgt gttcgcccgt
     2461 cagaccgagc cgcagatcgt gtcgacgtac gtcgcgaagg gacaggtgaa gatcgccttc
     2521 cacaacttcg ccttcctcgg ccccgagtcc aactgggccg cggaggcggc gctctgcgcc
     2581 ggcgagcagg gcaagttctt cgagttccac gaccggctgt tcgcgtcgca ggccggcgag
     2641 aacaatggcg cgttcgcgaa ggacaagctg aagaagatgg gcacggacct cgggctcagt
     2701 ccatcgttcg ccgcctgcgt cgactccggg aagtacgccc agtccgtccg cgacgagcag
     2761 aacgtcggtc agggcaagga cgtgagcagg accccgacgc tcttcgtgaa cggcaagaaa
     2821 tacgagggtg cgtactcctt cgacgagatg aagaagatca tcgagccgct cctcgccggc
     2881 ggccgctgat cctcggtcgt cgcgccgcgc tcgtcctcgc gacgatcggc acggccgtct
     2941 cggcctacct cacctggatc cactacagcg gcgcgttcgc gttctgcgcg ggcgtcggtg
     3001 actgcgaggt cgtccagacc agccgctact cgatgctcgg accgatcccc gtcgcgctca
     3061 tcggcctcgt cgggatggtg ctcatcctcg gcgtgtccct cggccggctc atgagcgacg
     3121 acccgcgcct cgacctcgcg ctcttcgcgt tcgcactcat ggcgaccgcc tacgtcgcgt
     3181 acctctccta cgtcgaggtg ttcgtgctcg gcgccatctg tccgtggtgc gtggcggtcg
     3241 cggtgtgctc cgtcgccatc ttcgccctcg ccgcgcgcga cgtcatgagc gcgtcatcct
     3301 gagcacgcgc cgcgcgcgct cgacgatcgg cgggtcgagc atctcgccat cgagcgcgac
     3361 cgcgccgagg cccttcgcca cgccatcctc gtaggccgcg acaacgcgcc gcgcgtgcgc
     3421 cacctcgtcg ggcgccggcg agaagatctc gttcaccacc gcgatctggt ccggatggat
     3481 gcactgccgg ccggagaagc cgagacggcg gccgcgcatc gcgtcgtcgc gcagaccttt
     3541 ttcgtcgcgg aagtccgccc agatgccgtc gaacgcgagc actcccgcgg ccaccgccgc
     3601 gacgaccacc gcggaccgcg c
//
LOCUS       DASTZR010000385         7534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_21663, whole genome shotgun sequence.
ACCESSION   DASTZR010000385 DASTZR010000000
VERSION     DASTZR010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7534
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21663"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VFV20_08620"
     CDS             complement(<1..488)
                     /locus_tag="VFV20_08620"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU5288452.1"
                     /translation="MTQPPQLVKVTIDDVTVEVPRNSLLIEAAGKAGVHVPRFCYHPK
                     LSLAGACRLCSVSVEKMPKLQTACTTPVADGMVARTNTPEVADARRSVLEFLLLNHPL
                     DCPVCDAAGECPLQDYSHLYGPNASRFALPKRRAVKAERLSPLVVLDMERCILCYRCT
                     RF"
     gene            complement(485..1771)
                     /gene="nuoF"
                     /locus_tag="VFV20_08625"
     CDS             complement(485..1771)
                     /gene="nuoF"
                     /locus_tag="VFV20_08625"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEU5288453.1"
                     /translation="MSAAYEPVLLHRRGLERSGDIDVYERTGGYQALRKAVGSMTPQA
                     VIDEVIASNLRGRGGAGFPTGRKWSFIPKDAPVKYLAVNADESEPGTFNNRVFIDADP
                     HALLEGILICCFAVGISAAYIYIRGENVAGARTLERAIAQAREKGYVGRGILGTAFSC
                     EIHVFRGAGAYICGEETALLESIEGRRPQPRSRPPFPAVVGLFGKPTVINNVETLGCV
                     PSIVERGAAWFKSIGPENAPGPKIYCLSGHVARPDVYERPMGTPLRELIEEHGGGIRG
                     GRSLGFVIPGAAASTALTSDEIDVAMDFDSLRKIGTELGAGGIMVFDDRVCIVETLER
                     LARFFRHESCGKCVPCREGTVWQHAILTRLAEKRGSAADIALLKSVSEQISGKTLCAL
                     GDFAAAPQLGAIKKFPEHFDAHVAGRCPTRGGALVA"
     gene            complement(1768..2214)
                     /locus_tag="VFV20_08630"
     CDS             complement(1768..2214)
                     /locus_tag="VFV20_08630"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEU5288454.1"
                     /translation="MDSIDSLAAKYPTMRAALLPALWLVQEERGWVSRADVAEVAERL
                     GLAAADAEGVISFYFLFHRSPRGRVVIDVCDNVVCQANGAEELLRDLCARLGIGPGET
                     TPDGRFTVRRQECIAACHQAPAVQVDLAYRGPITSVDDLLRPDHAR"
     gene            complement(2403..3605)
                     /gene="nuoD"
                     /locus_tag="VFV20_08635"
     CDS             complement(2403..3605)
                     /gene="nuoD"
                     /locus_tag="VFV20_08635"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEU5288455.1"
                     /translation="MTSVDHERGTMPPHETMLLNMGPQHPSTHGVLRLMLELDGEVIV
                     DAHPDIGFLHTGIEKSMEYRTYHQAITLTDRIDYLSPYSNNLAYVNAVERLFEVTPPP
                     RASWLRVLIAELHRIASHLVFIGTTGIDLAASSMLIYCFREREEILRLMELLSGVRMM
                     TSFIRIGGLPADVPPGFLDGVSSFLDRFPARLAEYDELLQENPIFKQRMIGIGKISAR
                     DAVRWSITGPSLRGSGVAYDVRRARPYEIYDQLEFDVISETAGDAYARYLVRRRELVE
                     SVKICRQVLARLPEGPVRIDDPLIAYPKRELIHRSMESLIHHYKLATDGPIPPPGEAY
                     AAVESPRGEYGVHVVSNGTNLPQRVRVRAPSFANLQALPLLVRGHMVADCVAALASID
                     FVLGEIDR"
     gene            complement(3602..4063)
                     /locus_tag="VFV20_08640"
     CDS             complement(3602..4063)
                     /locus_tag="VFV20_08640"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEU5288456.1"
                     /translation="MIRVADGIPTAVVAAEQLVERVTELRDDGYDLLVDVFAYDTPER
                     ERRFDVVYLFHRLADSARMRLHVEVGERDAVPSIVGLYPGASWYEREVFDMYGVSFTG
                     HPDLRRILMPDDWIGHPLRRDHPLGGEVVDYGLGPNVDTVAPIDFPEGTTR"
     gene            complement(4060..4539)
                     /locus_tag="VFV20_08645"
     CDS             complement(4060..4539)
                     /locus_tag="VFV20_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEU5288457.1"
                     /translation="MGGAQSQGMVFATVERAANWARSNSLWPLSFGLACCAIEMMSMS
                     AARFDVSRFGSEIFRVSPRQADLMIVAGRVSQKMAPVLRQLYDQMPEPKWVIAMGACA
                     SCGGVYNNYALVQGVDKIVPVDVFLAGCPPRPEALIFSLMQLQEMVKRGERRTIAVQ"
     gene            complement(4653..5009)
                     /locus_tag="VFV20_08650"
     CDS             complement(4653..5009)
                     /locus_tag="VFV20_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEU5288458.1"
                     /translation="MLAEYLPVLVLFVLVSLIGLVAVNIPHLIAPSRPSLAKLAAYES
                     GMSPIGPAWRRVPIRFYLTAMLFVLFDVEIVFFYPWAVALHRLRLFGLLEMAVFAGLL
                     LLGYAYVWRKGALRWE"
     gene            5142..5215
                     /locus_tag="VFV20_08655"
     tRNA            5142..5215
                     /locus_tag="VFV20_08655"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5176..5178,aa:Pro,seq:cgg)
     gene            5352..6290
                     /locus_tag="VFV20_08660"
     CDS             5352..6290
                     /locus_tag="VFV20_08660"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU5288459.1"
                     /translation="MAEVARRRQFGLHATGGRIGHLLDSEKVFGYVLVAPAIAYILLL
                     VAYPFGIALWFTMSNATVGQPIGEFTGLDNVRAVLDDDIFRRALLNTFAFTLISQALQ
                     MFFGTFLAFLLLRKFRGRRIARALIMLPFTIPVAIGAMAWTWMLNPTYSVINWLGEHA
                     GLFTEGPNWLGEEVPAMISIILVNVWRNLPFSAIVLTAGISSVPAEILEAADLDGANW
                     WVRWNKIMVPIIAPVLYIALLFSLVFTSTDMTVVWLMTRGNPVNATHVIASYAFQTGV
                     VAGSLGRGATMSLFLFPVLLVAAFLFLRNIKARSVD"
     gene            6290..7165
                     /locus_tag="VFV20_08665"
     CDS             6290..7165
                     /locus_tag="VFV20_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU5288460.1"
                     /translation="MAQTRIAHHSTAYRLRRLRGSVLFWGTLSLYLIFAAFPVYWMFV
                     TTFKQVNDLYNLENNPLLFHLAPSLDQVRYLFERTNFTTWVSNTAQVGLAVVIITVLI
                     CVPAAYVLSRTKFPGSGTLGIGIFLTYLVPPTLLFLPLSQLVVGLGLVNNKWALALVY
                     PTFTIPFCTWLLMGFFKGVPREITEAARVDGCSRLGAMFRIVLPVSVPGILTIVIFAF
                     TLAMQEYVYALTFVSSSDEKMITLGVVTDLIRGDVFFWGSLMAGALIVSIPVAIVYNM
                     FMDSFVRGITGGALK"
     gene            complement(7270..>7534)
                     /locus_tag="VFV20_08670"
     CDS             complement(7270..>7534)
                     /locus_tag="VFV20_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288461.1"
                     /translation="NPFLKGHQKKPMPVLGEDPKLTHLQDIANFVQPVGYPGPSSQAA
                     YDALNNHVVTDMFSNFITGKKTADETIAEAKKRLEDSLKKFPV"
BASE COUNT         1180 a         2587 c         2525 g         1242 t
ORIGIN      
        1 atgaagcgcg tgcagcgata gcagaggatg cagcgctcca tgtcgagcac caccagcggc
       61 gacagacgct cggccttcac ggcgcggcgt ttcggcagcg cgaagcgcga cgcgttcggt
      121 ccgtagagat gcgaatagtc ctgcagcgga cactccccag cggcgtcgca caccggacag
      181 tcgagggggt ggttgagcag caggaactcg aggaccgacc ggcgggcgtc ggcgacctcc
      241 ggcgtgttgg tgcgcgcgac catcccgtca gcgaccggcg tcgtgcaggc ggtctgcagc
      301 ttcggcatct tctcgaccga caccgagcac aggcggcacg cacccgcgag cgagagcttg
      361 gggtgatagc agaaccgtgg gacgtgcacg cccgcctttc ccgcggcctc gatgagcagg
      421 ctgttgcgcg ggacctcgac ggtgacgtcg tcgatggtga ccttcacgag ctgcggaggc
      481 tgcgtcatgc gaccagcgct ccgccgcgcg tcgggcaccg tccggcgacg tgcgcgtcga
      541 agtgctcggg gaacttcttg atcgcgccga gctgcggcgc cgccgcaaag tcgccgagcg
      601 cgcacagcgt cttcccggag atctgctcgc tcacgctctt cagcagcgcg atgtcggcgg
      661 cgctgccgcg cttctcggcg agccgcgtga ggatcgcgtg ctgccagacc gtgccctccc
      721 ggcagggcac gcacttcccg caggactcgt ggcggaagaa tcgcgcgagc cgttcgagcg
      781 tctcgacgat gcacacacga tcgtcgaaca ccatgatccc gccggcgccc agctcggtgc
      841 cgatcttccg caacgaatcg aagtccatcg cgacatcgat ctcatcggag gtcagcgcgg
      901 tggacgccgc ggcacccggg atgacgaatc cgagcgagcg cccgccgcgg atgcccccgc
      961 cgtgctcctc gatgagctcg cgcagcggcg tccccatcgg ccgctcgtag acgtccggcc
     1021 gcgccacgtg tcccgagagg cagtagatct tgggacccgg cgcgttctcc ggtccgatgc
     1081 tcttgaacca ggcggcgccg cgctcgacga tcgacgggac gcagccgagc gtctcgacgt
     1141 tgttgatcac ggtgggcttg ccgaagaggc caacgaccgc cgggaacggc ggacgcgacc
     1201 gcggctgtgg gcggcggcct tcgatggact cgaggagcgc ggtctcctcg ccgcagatgt
     1261 aggcgcccgc gccgcggaag acgtggatct cgcatgagaa cgccgtgccg aggatgccgc
     1321 ggccgacata gcccttctcg cgcgcctggg cgatcgcacg ctcgagcgtg cgcgctccgg
     1381 cgacgttctc gccgcggatg tagatgtacg cggccgagat cccgacggcg aagcagcaga
     1441 tgaggatgcc ctcgagcagg gcgtgtgggt ccgcgtcgat gaagacgcgg ttgttgaacg
     1501 tgcccggctc gctctcgtcc gcgttcaccg cgaggtactt caccggcgcg tccttcggga
     1561 tgaacgacca cttgcgaccc gtggggaaac ccgcgccgcc gcgcccgcgt aggttcgagg
     1621 cgatgacctc gtcgatcacc gcctgcggcg tcatcgagcc gaccgccttg cgaagcgcct
     1681 gatatccgcc ggtgcgctcg tacacgtcga tgtcgcccga gcgctcgagc ccgcggcggt
     1741 gaaggaggac cggctcgtat gccgcgctca tcgtgcgtgg tccgggcgga gcaggtcgtc
     1801 cacgctggtg atgggtccgc ggtaggcgag atccacctga acggcgggtg cctggtggca
     1861 cgcggcgatg cactcctgcc ggcgcaccgt gaagcgcccg tcgggcgtcg tctcccccgg
     1921 tccgatgccg aggcgcgcgc acaggtcgcg caggagctcc tcggcgccgt tggcctgaca
     1981 cacgacgttg tcgcagacgt cgatgacgac ccggccgcga ggcgagcggt ggaacaggaa
     2041 gtagaacgag atcacgccct ccgcgtccgc tgcggcgagg ccgagccgct ccgcgacctc
     2101 ggcgacgtcc gcacgcgaca cccatccccg ctcctcctgc accaaccaga gcgccgggag
     2161 cagggcggct cgcatcgtcg ggtacttcgc cgcgagcgaa tcgatcgaat ccaacagcga
     2221 gctcgggagc ggtggtctcg ttgacgacga ctccgaaggc ggctcgccgg accgagggcc
     2281 accgcgcggg gagggattcc cgtcgcgacc agcgacggaa taccccgcgg agagagggtt
     2341 tcggtccggc gagccgccgc gccgagtggc gtcgtcacga ccaccggggt cccgctgcga
     2401 ggtcatcgat cgatctcgcc gaggacgaag tcgatcgatg cgagcgctgc gacgcagtcc
     2461 gccaccatgt gtccgcggac caggagcggg agcgcctgca ggttcgcgaa ggagggcgcg
     2521 cggacgcgga cgcgctgcgg caggtttgtc ccgttcgaca cgacgtgaac gccgtactcg
     2581 ccccgcggcg actcgacggc cgcgtacgcc tcgccgggcg gcgggatcgg cccgtcggtc
     2641 gcgagcttgt agtgatggat gagggattcc atcgagcgat ggatgagctc gcgcttcgga
     2701 tacgcgatga gcgggtcgtc gatccggacc ggaccctcgg gcaggcgcgc gagcacctgg
     2761 cggcagatct tcacgctctc gacgagctcg cgccgtcgca ccaggtagcg cgcgtacgcg
     2821 tccccggcgg tctcgctgat cacgtcgaac tccagctggt cgtagatctc gtacggacga
     2881 gcgcggcgga cgtcgtacgc gacgcccgat ccacgcaggc tcggcccggt gatgctccag
     2941 cgcaccgcgt cgcgcgcgga gatcttgccg atgccgatca tgcgctgctt gaagatcggg
     3001 ttctcctgca gcagctcgtc gtactccgcc agccgcgccg gaaaccggtc caggaacgac
     3061 gagacgccgt cgagaaagcc gggcgggacg tcggccggca gtccgccgat ccggatgaag
     3121 ctcgtcatca tgcgcacgcc ggagagcaac tccatcaggc ggaggatctc ctcgcgctcg
     3181 cggaagcagt agatgagcat gctcgatgcc gcgaggtcga tgcccgtcgt cccgatgaag
     3241 accagatgcg acgcgatgcg gtgcagctcg gcgatgagca cacgcaacca cgaagcgcgc
     3301 gggggcggtg tcacctcgaa gagccgctcg accgcgttca cgtacgcgag gttgttcgag
     3361 tacggcgaca ggtagtcgat gcggtcggtg agggtgatgg cctggtggta ggtgcggtac
     3421 tccatcgact tctcgatccc cgtgtgcagg aagccgatgt cggggtgcgc gtcgacgatg
     3481 acctccccgt cgagctcgag catcagccgc agcacaccgt gggtcgacgg gtgctgcggg
     3541 cccatgttca gcagcatcgt ctcgtgcggc ggcatggtgc cccgctcgtg atcgacggag
     3601 gtcatcgcgt cgtgccttcg gggaagtcga tcggcgccac cgtgtcgacg ttcggaccga
     3661 gaccgtagtc cacgacctct ccgcccagcg ggtggtcgcg tcgcagcggg tggccgatcc
     3721 agtcgtccgg catcaggatg cgccgcaggt cggggtgccc ggtgaaggac acgccgtaca
     3781 tgtcgaagac ctcgcgctcg taccagctcg cacctggata gaggccgacg atgctcggca
     3841 ccgcgtcgcg ctcccccacc tcgacgtgca accgcatgcg tgcgctgtcg gccagacggt
     3901 ggaagaggta cacgacgtcg aagcgccgct cgcgctcggg cgtgtcgtac gcgaacacgt
     3961 ccaccagcag gtcgtacccg tcatcgcgga gctcggtcac ccgctccacg agctgctccg
     4021 ccgcgacgac ggccgtcggg atgccgtcgg cgacgcggat cattggacgg cgatcgtcct
     4081 gcgctcgccg cgcttcacca tctcctggag ctgcatgagc gagaagatga gcgcctccgg
     4141 ccgaggcgga cagccggcga ggaacacgtc gaccgggacg atcttgtcga cgccctgaac
     4201 gagcgcgtag ttgttgtaga cgccgccgca cgacgcgcag gcgcccatgg cgatgaccca
     4261 tttcggctcc ggcatctggt cgtagagctg ccggagcacc ggcgccatct tctgcgacac
     4321 ccgcccggcc acgatcatga ggtcggcctg ccgcgggctc acgcggaaga tctccgagcc
     4381 gaagcgagag acgtcgaagc gggcggcgga catgctcatc atctcgatcg cgcagcacgc
     4441 caggccgaag gagagcggcc acagcgagtt cgaccgcgcc cagttcgcgg cccgctcgac
     4501 cgtggcgaac accatgccct ggctctgcgc gccgcccatg ccccccggct gctggatcag
     4561 cgcttcgcgc tgacctcttg ccctaccact cgttgtcact cgtgcatccg actcgctcgt
     4621 cgcgggggac ccgctcctcg ctcgacttcg gtctattccc atctgagcgc tcccttgcgc
     4681 cagacgtagg cgtagccgag cagcagcagg cccgcgaaga ccgccatctc gagcagcccg
     4741 aagagacgca gccggtgcag ggccacggcc cacgggtaga agaagacgat ctccacgtcg
     4801 aagagcacga agagcatcgc cgtcaggtaa aagcggatcg gcacccgccg ccatgccggg
     4861 ccgatggggc tcatgcccga ctcatacgcg gcgagcttgg ccagcgacgg ccggctcggg
     4921 gcgatgagat gcgggatgtt cacggccacc agcccgatga gggagacgag cacgaagagg
     4981 acgagcacgg ggaggtactc agcgagcatc gacacgacga ttgtagggac gatccgatcg
     5041 cgtcgaatct cgatcgccac ggcctcacgc ccggtcgtcg cgctcacgaa cccctacaat
     5101 cgagttcgcc atcggtgcct tccccgcggc accggatcca tcggggtgta gcgcagcttg
     5161 gtagcgcgca gcgttcggga cgctgaggtc ccgggttcga gtcccggcac cccgacacgc
     5221 tagcgaggac gaggcagcga gccgcagatc gctgctgagg ccgagcgacg cgagtatggg
     5281 gccgccatac cagcgaccaa ccgaccaacg ccgcgtgtct tacgcctgcg ctcgtctatc
     5341 ttgtgagcga cgtggcagaa gtggcgcggc ggcgtcagtt cgggctccat gcgaccggcg
     5401 gtcgcattgg ccatcttctc gacagcgaga aggtgttcgg ctacgtcctc gtcgcgccgg
     5461 ccatcgcgta catcctgctc ctcgtcgcgt acccgttcgg catcgcgctg tggttcacga
     5521 tgagcaacgc caccgttgga cagccgatcg gcgagttcac gggcctcgac aacgtgcggg
     5581 cggtgctgga cgacgacatc ttccgacgcg cgctgctcaa caccttcgcc ttcacgctga
     5641 tctcgcaggc cctccagatg ttcttcggga cgttcctcgc attccttctg ctgcgcaagt
     5701 tccgcggccg ccgcatcgcg cgcgcgctca tcatgctgcc gttcacgatc cccgtcgcga
     5761 tcggcgcgat ggcctggacc tggatgctca acccgacgta cagcgtcatc aactggctcg
     5821 gggagcacgc gggtctcttc accgagggcc ccaactggct cggtgaggaa gtgcccgcga
     5881 tgatctcgat catcctcgtc aacgtctggc gcaacctgcc cttcagcgcg atcgtgctca
     5941 cggcaggcat cagctcggtg ccggcggaga tcctcgaggc cgcggacctc gacggcgcga
     6001 actggtgggt ccgctggaac aagatcatgg tcccgatcat cgcgccggtg ctgtatatcg
     6061 ccctgctctt ctcgctcgtc ttcacctcga cggacatgac ggtcgtgtgg ctcatgacgc
     6121 gcgggaatcc tgtgaacgcc acgcacgtga tcgcctcgta cgcgttccag acgggcgtcg
     6181 tcgccggctc gctcggccgc ggcgcgacga tgtccctgtt cctcttcccg gtcctgctcg
     6241 tcgccgcctt cctgttcctg cgcaacatca aggcgcggtc ggtggactga tggcgcagac
     6301 gcggatcgcg caccactcga ccgcgtaccg gctgcggcgg ctacgcggct ccgtgctgtt
     6361 ctggggcacg ctgtcgctct acctgatctt cgccgccttc cccgtctact ggatgttcgt
     6421 cacgacgttc aagcaggtga acgacctcta caacctcgag aacaacccgc tgctgttcca
     6481 cctggcgcca tcgcttgacc aggtccggta cctcttcgag cggacgaact tcaccacctg
     6541 ggtctcgaac accgcgcagg tcggcctcgc ggtggtcatc atcaccgtgc tgatctgcgt
     6601 tcccgcggcc tacgtgctct cgcgcacgaa gttccctggc tcgggcacgc tcggcatcgg
     6661 catcttcctc acgtatctcg tgccgccgac gctgctgttc ctcccgctgt cgcagctcgt
     6721 cgttggtctc gggctcgtga acaacaagtg ggcgctcgcg ctcgtctatc ccacgttcac
     6781 gatccccttc tgcacctggc tcctcatggg cttcttcaaa ggcgtgccgc gcgagatcac
     6841 cgaggcggca cgcgtggacg gctgcagtcg cctgggcgcg atgttccgca tcgtcctgcc
     6901 ggtgagcgtc ccggggatcc tcaccatcgt gatcttcgcc ttcaccctgg cgatgcagga
     6961 gtacgtctac gcgctgacgt tcgtgtcgtc gtcggacgag aagatgatca cgctcggcgt
     7021 cgtcaccgac ctcatccgcg gcgacgtctt cttctggggc tccctcatgg ccggcgcgct
     7081 catcgtttcg ataccggtgg ccatcgtcta caacatgttc atggacagct tcgtccgtgg
     7141 catcaccggc ggcgcgctga agtagcggtc aacgcagggc gcgagtcgcg cgccgtgcac
     7201 gcaagaggcc ggacctgttc ggcccggcct cctcgctgct cccacccgcg gtgatgcggg
     7261 ccgcggtcgc tagaccggga acttcttgag cgagtcctcg aggcgcttct tcgcctcagc
     7321 gatggtctcg tcggccgtct tcttgccggt gatgaagttg ctgaacatgt cggtgacgac
     7381 gtggttgttg agcgcgtcgt acgccgcctg cgaggacggc cccgggtagc cgacgggctg
     7441 cacgaagttc gcgatgtcct gcaggtgcgt cagcttcggg tcctcgccga ggaccggcat
     7501 cggcttcttc tggtgaccct tgaggaacgg gttg
//
LOCUS       DASTZR010000386         3726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1295425, whole genome shotgun sequence.
ACCESSION   DASTZR010000386 DASTZR010000000
VERSION     DASTZR010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295425"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1532)
                     /gene="recN"
                     /locus_tag="VFV20_08675"
     CDS             complement(<1..1532)
                     /gene="recN"
                     /locus_tag="VFV20_08675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEU5288462.1"
                     /translation="MKDLALVSELRLSLSPGLTVLTGETGAGKSMLVDALLAVTGGRV
                     PSGMVREGSERAYVEAVFAGARDDDLVLSREIAVGRSPARIDQKTVPLSSLADVGGDL
                     LAIHGQGDQLRLARANVQRDVLDAFGGHQALRDRCAEAAALLSALDRERAALGGDPRE
                     RERRAVLLAHEVEEIRGAKLRPDEETQLEAELAVARSAEKLREAAHAAQELLAGDRAS
                     ARDRLALAAREVRRAAEMDARLTATYERVAAALAEADEIASELRRYADSIETDPRRLA
                     ELEARSDLLHDLRRKYGEDVGAIVAYGERAAADLETLRKSETRLAQLDADEAKARAAL
                     AKAARSLHDARAKAGAALAAAVESQLKDLALARCRFSIRLAERAPDATGGDDVRFLIA
                     PNPGEPAKPIDEIASGGELSRIMLALEVTLAASDRTPVLVFDEVDAGVGGRVGEIVGR
                     ALWSLGRHHQVMVVSHLPQVAAYADHHIAVRKELRKGRTHVVAAPLAEREVVEELADM
                     LGA"
     gene            complement(1600..1890)
                     /locus_tag="VFV20_08680"
     CDS             complement(1600..1890)
                     /locus_tag="VFV20_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU5288463.1"
                     /translation="MAGISKSETLNRLAEKTGLAKKQVASVLEELASLSYKEARNGFT
                     FPGVGKLVVVDRKARLGRNPATGETIQIPAKRVLKFRIAKQAKDAVLAKRGK"
     gene            2004..2501
                     /locus_tag="VFV20_08685"
     CDS             2004..2501
                     /locus_tag="VFV20_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288464.1"
                     /translation="MTLTVTDEPPDRPEWVSETIRLVAEATAEWRDRLLALVDRASDA
                     DLSAGTDEAWGVGQVAVHVLLIERGVAGIALRLARGEPPGRTGQPRPAAAAVSRAGIA
                     SLAAKSADAMAGLGAEFPPAPDIRAVARHPYYGELNTFGWLLTLPNHYRAHLDALERG
                     TKSAL"
     gene            2570..>3726
                     /locus_tag="VFV20_08690"
     CDS             2570..>3726
                     /locus_tag="VFV20_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU5288465.1"
                     /translation="MATTKTAALETFAQEDRSFAPPREFAAKANVTDPAIYERAEKDL
                     EGFWAEQARTLTWRKPFSKTLEWAAPYAKWFVGGELNISENCLDRHVKAGKGTKVAYH
                     WEGEPGDTRTITYQDLLDETSRCANALRSLGVRKGDRVAIYMPMIPELPAAMLACARI
                     GAPFTVVFGGFSAEALAGRINDSEAKVLITADGGYRKGNIVPLKKNADDAIAQTKTIE
                     KVLVVRRTKNDVPWTDGRDVWWHDLVEKQSTDCPAEPVDSEHMLYLLYTSGTTAKPKG
                     IVHTTAGYLAGVATTHKMIFDIKEDDVYWCAADIGWVTGHSYIVFGPLANGTTGVMYE
                     GTPDFPDKDRFWRIIEKHGITICYTAPTAIRTFMRWGEQYPRRCDLSSLRLL"
BASE COUNT          579 a         1335 c         1283 g          529 t
ORIGIN      
        1 gccccgagca tgtcggcgag ctcctccacg acctcgcgtt ccgcgagcgg cgcggcgacc
       61 acatgggtgc ggcccttccg gagctccttg cgcaccgcga tgtggtgatc cgcgtatgcc
      121 gcgacctggg ggagatggct cacgaccatg acctgatggt gacgccccag cgaccagagc
      181 gcgcggccga cgatctcgcc gacgcgcccg cccacgccgg cgtcgacctc gtcgaacacg
      241 agcacgggcg tgcggtcgct tgccgccagg gtcacctcga gcgcgagcat gatgcgcgac
      301 agctcgccgc cggacgcgat ctcatcgatg ggcttcgccg gctcaccggg attcggtgcg
      361 atgaggaagc ggacgtcgtc gccgccggtc gcgtccggtg cgcgctccgc gagccggatg
      421 ctgaagcggc accgcgcgag cgccagatcc ttgagctgcg attcgaccgc ggcggcgagc
      481 gccgcacccg ccttcgcgcg cgcgtcgtgc agcgaccgcg ccgccttcgc gagcgcggcg
      541 cgcgccttcg cctcgtccgc atcgagctgc gcgaggcgcg tctcgctctt gcgcagcgtc
      601 tcgaggtccg ccgccgcccg ctcgccgtac gcgacgatcg cgccgacgtc ctcgccgtat
      661 ttgcgccgca ggtcatggag caggtctgat cgcgcctcga gctccgccag acgcctcgga
      721 tccgtctcga tgctgtcggc gtagcggcgg agctcggatg cgatctcgtc cgcctccgcc
      781 agtgcggcgg ccacgcgctc gtacgtcgcg gtaaggcgcg cgtccatctc cgccgctcgc
      841 cggacctcgc gtgccgcgag cgcgagacga tcgcgcgcgc tcgctcgatc gccggcgagc
      901 agctcctgcg cggcgtgcgc cgcctcgcgc agcttctccg cggaacgcgc gacggcgagc
      961 tccgcctcca gctgcgtctc ctcgtccggc cgcagcttcg cgccgcggat ctcctcgacc
     1021 tcgtgcgcga gcagcacggc acggcgctcg cgctcccgtg gatcgccacc cagcgccgcg
     1081 cgctcgcggt cgagcgcgga gagcagcgcg gccgcctccg cgcagcggtc gcgcagcgcc
     1141 tggtgtccgc cgaacgcgtc gagcacgtcg cgctgcacgt tcgcgcgcgc gaggcgcagc
     1201 tgatcgccct gaccgtgtat cgcgaggaga tcgccgccga cgtcggcgag cgacgacagc
     1261 ggcaccgtct tctgatcgat gcgcgcgggc gaacgtccca cggcgatctc gcgagagagc
     1321 acgaggtcgt cgtcgcgcgc gccggcgaag acagcctcaa cgtacgcgcg ttccgagccc
     1381 tcgcgcacca tgccgctcgg cacgcgtccg ccggtcacgg cgaggagcgc gtccacgagc
     1441 atcgactttc ccgcgccggt ctcgccggtg agtacggtga gtccggggga gagcgagagg
     1501 cggagctccg aaacgagagc gaggtccttc accgagagct ccgcgatgta cggaaccaca
     1561 ggtgaaggac ctcgcttcaa gaagagtgcg tgtgtctgac tacttgccgc gcttcgcgag
     1621 gacggcgtcc ttcgcctgct tggcgatgcg gaacttcaga acgcgcttcg ccgggatctg
     1681 gatcgtctcg ccggtcgcgg ggttgcggcc gagccgcgcc ttgcggtcga cgaccacgag
     1741 cttgccgacg cccgggaagg tgaagccgtt gcgggcttcc ttgtacgaaa gcgatgcgag
     1801 ctcctcgagc acgctcgcga cctgcttctt cgccagtccg gtcttctccg cgagccggtt
     1861 caaggtctcc gacttcgaga tccccgccac ttcgtcctcc taaggcccca gggccatatt
     1921 tgcttggttc gcggcgcatt ctcacacatc gagcgagagg gagcgcgcgg caaggcccgg
     1981 aatccgggca ctatcagtac gacatgaccc tgaccgtgac cgatgaaccc ccggaccgcc
     2041 cggagtgggt gagcgagacc atccgtctgg tcgccgaggc gacggccgaa tggcgtgacc
     2101 ggctcctcgc gctcgtggac cgggcgagcg acgccgacct cagtgcggga acggacgagg
     2161 cgtggggcgt cggccaggtc gccgtccacg tcctgctcat cgaacggggc gtcgccggca
     2221 tcgcgctccg cctcgcgcga ggcgaaccgc ccggtcgtac cggacagccg cgtccggcag
     2281 cggccgcggt ctctcgcgcg gggatcgcca gcctggccgc caagtcggcg gacgccatgg
     2341 ccgggctcgg ggcggaattt ccgcccgcgc cggacattcg cgccgtcgcg cgccacccgt
     2401 actacgggga gctgaacacc ttcggctggc tgctcacgct ccccaaccac taccgggcgc
     2461 atcttgacgc cctggagcgc gggacgaaaa gcgccctgta ggcgaacacg cgccgcgacc
     2521 tggcggcata ctcgccagcc aaggctacgc acaccggagg gacgacggta tggccacgac
     2581 caagacggcc gcgctcgaga cgttcgccca ggaggaccgc agcttcgcgc cgccgcgtga
     2641 gttcgccgcc aaagcgaatg tcacggatcc cgccatctac gagcgtgcgg agaaggacct
     2701 cgaggggttc tgggccgagc aggcgcggac cctcacctgg cggaagccgt tcagcaagac
     2761 gctcgagtgg gccgcgccgt acgcgaagtg gttcgtgggc ggcgagctga acatctcgga
     2821 gaactgcctc gaccgtcacg tgaaggccgg caagggcacg aaggtcgcgt accactggga
     2881 gggcgagccg ggagacacgc gcacgatcac ataccaggac ctgctggacg agacctcccg
     2941 ctgcgcgaac gcgctgcggt cgctcggggt gcgcaagggc gaccgcgtcg cgatctacat
     3001 gccgatgatc ccggagctac ccgcggcgat gctcgcctgt gcccgcatcg gcgccccgtt
     3061 cacggtggtc ttcggcggct tcagcgccga ggccctggcg ggccgcatca atgactccga
     3121 ggcgaaggtg ctgatcaccg ccgacggcgg ctaccgcaag ggcaacatcg tgccgctcaa
     3181 gaagaacgcc gacgatgcca tcgcgcagac gaagaccatc gagaaggtgc tggtcgtgcg
     3241 ccgcacgaag aacgacgtgc catggacgga cggccgcgac gtctggtggc acgacctcgt
     3301 cgagaagcag agcacggact gccccgcgga gcccgtcgac agcgagcaca tgctctacct
     3361 gctctacacc tcggggacca ccgcgaagcc gaaggggatc gtgcacacga cggcggggta
     3421 cctggccggc gtcgcgacga cgcacaagat gatcttcgac atcaaagagg acgacgtgta
     3481 ctggtgcgcg gccgacatcg gctgggtgac cggtcactcc tacatcgtgt tcggcccgct
     3541 cgcgaacggg accaccggcg tcatgtacga gggcacgccg gacttcccgg acaaggaccg
     3601 attctggcgg atcatcgaga agcacgggat cacgatctgc tacacggcgc cgaccgccat
     3661 ccggacgttc atgcgctggg gggagcagta tccccgtcgc tgcgatctct cgagcctgcg
     3721 gctgct
//
LOCUS       DASTZR010000387         2425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_517779, whole genome shotgun sequence.
ACCESSION   DASTZR010000387 DASTZR010000000
VERSION     DASTZR010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517779"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="VFV20_08695"
     CDS             complement(<1..1127)
                     /locus_tag="VFV20_08695"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5288466.1"
                     /translation="MSAREQAEGGGYERRIRLASELATWGIAGLLVASAALPTTDAIA
                     RVGLLFSAALLGAFAILWFHAIPESMFGRLRFTIGTSITQVVAAVLLVLTGGAPSPYF
                     VFFLFPALATTFAMRISSTLVVGAIGLLLYLTILVSDALESHGSTALFDLGAIRVSAF
                     VALVAMTSLITRTMQDTRAVLRQRSNELALRNQELEISRSTALAIARARDLGELLRAV
                     HEAARSALAIDRVFFFSGPEADESGYTIGPDGTIEPFEPDRRPRDSPRQRAMRARGSV
                     IVNDIAAETMVNPRLRGHYHVGAGLFIPLSQRGELIGQIVLSVAAPRDWTPREVRLGE
                     VLAESAAPAIASYLTLEAVRAERETLAGRMKVLEGMNQLVEA"
     gene            1185..2309
                     /locus_tag="VFV20_08700"
     CDS             1185..2309
                     /locus_tag="VFV20_08700"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEU5288467.1"
                     /translation="MRIAKLATHVLEGKAERAIQFGAGSYGTFTATLVEVTTDDGITG
                     VGECIARRAPRVVDTVVRELLWPVVEGRDARDVGGLWDEMQARLRPWGHYRGFVQEGI
                     SGIDQALWDILAQAEGVPLYKALRGAGRERVPVYGSKVYTTDLPAMAREAEAAVKRGH
                     RAIKIQLGRSAEFGGPNADVEVCRTIREAVGPDIEMGADVNSAYDAGTAIRVCERIAK
                     YDLWFLEEPVFPDDLDGYERIRRASSVPLACGESEFGTFGFRELFRRGLIDVVQPDVA
                     RVGGFTAAMRVGAMVQAYNLRYAPHTGFSCGVAQVASLHLSAAVPNLARCEWMWIDNP
                     LAEIFEEPLPQPRDGLITLPTRPGLGLTLDRKKLARFRVN"
BASE COUNT          385 a          821 c          893 g          326 t
ORIGIN      
        1 gcctccacca gctggttcat gccctccagc accttcatgc gaccggcgag cgtctcgcgc
       61 tcggcgcgga cggcctccag cgtgagatag gaggcgatcg ccggcgcggc ggactcggcg
      121 aggacctcgc caagccggac ctcgcgcggc gtccagtcgc gcggcgcggc cacggacagg
      181 acgatctgcc cgatgagctc gccgcgctga ctgagcggta tgaacaggcc ggcgccgacg
      241 tggtagtggc cacgcagccg cgggttcacc atcgtctccg ccgcgatgtc gttcacgatc
      301 accgagccgc gcgcgcgcat cgcgcgctgg cgcggcgagt cgcgcgggcg acgatccggc
      361 tcgaagggct cgatcgttcc gtcgggaccg atcgtgtaac cgctctcgtc cgcctcgggg
      421 ccgctgaaga agaagacgcg gtcgatcgcg agcgccgagc gagcggcctc gtgcaccgcg
      481 cggagcagct cgccgagatc gcgagcgcgc gcgatcgcga gcgcggtcga gcgcgagatc
      541 tcgagctcct ggttgcggag ggcgagctca ttcgaacgct ggcgcagcac cgcgcgcgtg
      601 tcctgcatcg tgcgggtgat gagcgacgtc atcgccacca gcgcgacgaa cgccgacacg
      661 cggatcgcgc cgagatcgaa gagcgccgtg ctcccgtgcg actcgagcgc gtcgctcacg
      721 aggatggtca ggtagaggag cagcccgatc gcgcccacga cgagcgtgct cgagatccgc
      781 atggcgaacg tcgtggcgag tgccgggaag aggaagaaga cgaaataggg cgatggcgcg
      841 ccaccggtga ggacgaggag cacggcggcg acgacctgcg tgatgctggt accgatggtg
      901 aagcgcagcc ggccgaacat ggactcgggg atcgcgtgga accacaggat cgcgaacgcg
      961 ccgagcagcg cggcgctgaa gagcagaccc acgcgcgcga tcgcgtcggt ggtcgggagc
     1021 gccgccgatg cgacgagaag accggcgata ccccacgtcg cgagctccga cgcgagccgg
     1081 atgcgccgct cgtacccgcc accctccgcc tgctcccgcg cggacattcg ggcaatggtg
     1141 cgataactcg gttagcctgt cgcggtcgag cggaggtgaa cctcgtgagg atcgcgaaac
     1201 tcgcgacgca cgtgctggaa ggcaaagctg agcgcgccat ccagttcggc gcgggctcct
     1261 acggcacgtt cacggcgacg ctcgtcgagg tcacgaccga cgacggcatc accggcgtcg
     1321 gcgagtgcat cgcgcgacgg gcgccccgcg tcgtcgacac cgtggtgcgc gagcttctct
     1381 ggccggtcgt cgagggtcgc gacgcccgcg acgtcggcgg cctctgggac gagatgcagg
     1441 cacgcctccg gccgtggggg cactaccgcg gattcgtgca ggaaggcatc tcgggcatcg
     1501 accaggcgct ctgggacatc ctcgcgcagg ccgaaggcgt tccgctgtac aaggcgctgc
     1561 gcggcgccgg ccgcgagcgc gttcccgtgt acggatcgaa ggtgtacacc accgacctgc
     1621 ccgcgatggc gagggaggcc gaggccgcgg tgaagcgcgg tcaccgggcc atcaagatcc
     1681 agctcgggcg gtccgctgag ttcggcggtc cgaacgcgga cgtcgaggtc tgccgcacga
     1741 tccgcgaggc ggtcgggccg gacatcgaga tgggcgcgga cgtgaacagc gcgtacgacg
     1801 cgggcaccgc gatccgcgtg tgcgagcgca tcgccaagta cgacctctgg ttcctcgagg
     1861 agccggtgtt ccccgacgac ctggacggct acgagcggat ccgccgcgcc tcgagcgtgc
     1921 cgctcgcctg cggggagtcg gaattcggca cgttcggttt ccgcgagctg ttccgccgcg
     1981 gcctcatcga cgtcgtgcag ccggacgtcg cgcgcgtcgg agggttcacg gcggcgatgc
     2041 gcgtgggggc gatggtgcag gcgtacaacc tgcgctacgc gccgcacacc ggcttctcgt
     2101 gcggcgtcgc gcaggtggcg tcgctgcacc tctccgcggc ggtaccgaac ctcgcgcgct
     2161 gcgaatggat gtggatcgac aacccgctgg cggagatctt cgaggagccg ctgccgcagc
     2221 cgagggacgg cctcatcacc ctgccgacgc ggccgggtct cggactgacg ctcgaccgaa
     2281 agaagctcgc gcgcttccgc gtcaactgag gtgccgcgcg ggcctaaccc gctccggcga
     2341 gaatcaccgc tggatcgacg ggaccgctcg catcgtgcac ggcgaagtgc aggtgcgggc
     2401 cggtcgtgat gcccgtcatg ccgac
//
LOCUS       DASTZR010000388         4495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_68915, whole genome shotgun sequence.
ACCESSION   DASTZR010000388 DASTZR010000000
VERSION     DASTZR010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68915"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VFV20_08705"
     CDS             complement(<1..438)
                     /locus_tag="VFV20_08705"
                     /inference="COORDINATES: protein motif:HMM:NF013396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HEU5288468.1"
                     /translation="MTRFHVIGIGGAGMSAVARILLARGHDVTGSDTGAWPLADALAR
                     DGVAVHERFAPENVRGADIVIRSSAYGHDNVEVREATAQGIPVWKREDAWRSVTTGQR
                     VVAIAGTHGKSTTTAMTWMALRAGGIDASLICGAVLRGLGTNAH"
     gene            complement(435..1493)
                     /locus_tag="VFV20_08710"
     CDS             complement(435..1493)
                     /locus_tag="VFV20_08710"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEU5288469.1"
                     /translation="MLAIAEALRETDPEVRLQFIGGRRGLENKLVPEAGIPFHALPVA
                     SLRDPDSRFALIGTVLRLPFAYVDALARIAAFRPRVCVTSGGAIAFPVILAARTLRVP
                     VYLWAGDALPGRASRTLGRFCTKIGLAFEQARVHFPAGRATVTGTPIRSSLLRWTRES
                     AREKMGVPSEAPLVVVTGGSQGSERVNDTVFAALPRLLKSAYVLHVTGDAHIAKAKAR
                     EQSLTEEQRQRYLPHPYLRDEMGAVLAAADLVIGRAGASSIAEPLAFGTPLVLIPFGA
                     AMEGHQEANARAVAEAGAAVIVRESELDPDRLTAQVTGLLNDATRFGRMTQAAKNAGR
                     RDASKVMAAEILALGGCA"
     gene            complement(1408..2655)
                     /gene="ftsW"
                     /locus_tag="VFV20_08715"
     CDS             complement(1408..2655)
                     /gene="ftsW"
                     /locus_tag="VFV20_08715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEU5288470.1"
                     /translation="MKLHLPRTTSFDLTLVVVVLALTLLGIAMVYSASGIRALDTLDD
                     PRYYLTWQAIWAAIGLVGMVAAMRFDYHRYRVLAVPLVLLVVALLVLVLVPGLGVCAG
                     GACRWLRLGPVGLQPAELAKLALIVYAAVWLGGRRDRIATPGVALPLFGVTLVLAGLV
                     VREPDLGTAIVIVAIAMSMYFVAGARVKVFGALAALVAVVVLAVAIEQPYRLQRITTF
                     LDPWRDPRGDGFQTIQSLYGLALGGLFGEGLGAGREKFGFLPAPYTDSIFAVLGDELG
                     LAGTLAVIVLFFVLAYRGVAIALRARDATGALLAAGITTWLVFQAWVNMAVVASLVPM
                     TGITLPFISYGGSSLCVGLIAVGIQLNVGREAGPAPSVSRAPRRRRDGRSYQPAARDR
                     RGAPRDRSRGAAPVHRWSSRARE"
     gene            complement(2652..4109)
                     /gene="murD"
                     /locus_tag="VFV20_08720"
     CDS             complement(2652..4109)
                     /gene="murD"
                     /locus_tag="VFV20_08720"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU5288471.1"
                     /translation="MTVVFDAPITRDDLRGKRVTIVGLGKGRTSAGLAQFLVACGARV
                     TISDAAPRAERAEGIARLGDTPVEFVFGPSSDDSALADPDFVFIVPGIRPRSATILRA
                     LQRDIPVLTEIGLFFRLCPAPIVGVTGTKGKTTTTTMIEDILRRGQRRVISGGNIGRS
                     IIQELDGITRSDVVLLELSSFQLETLGTSPHVAVITNVSEDHLDHHGTRENYIAAKRN
                     ILAWQGSKDIAVLNLDDPVVVAMHTGSASEVRAYSLGLRPKRGAHLGAAGQLVLVDGP
                     RETALLAADELRVVGRHNIANALAAAIAGQAMGVPPGEIAAALRAFEGVPHRLQRVAE
                     IDGVLWVNDSAATTPAATLAALEAFDRRPVVILGGVSKGVEFGELGRALVRRARAAVL
                     LGPASDDIAASLDAAAPGPGAGRLPVRRAASLPDAIAAARELAQTGDVVLLSPACAAR
                     EDITHQKTVDAFTSADDRGDRFVALVRQLASGTGA"
     gene            complement(4106..>4495)
                     /locus_tag="VFV20_08725"
     CDS             complement(4106..>4495)
                     /locus_tag="VFV20_08725"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HEU5288472.1"
                     /translation="GGAHAGWEGTSHKVATALIEAMVAGGAARERLVAAIGPSIGPCC
                     YAIDDARAALVAERIGGNAIRRVDGRAVMDLWEANAAQLRAAGVVRIETAGVCTRSGG
                     ADVWSYRGRGPDGKYGTQLGFIGRLAS"
BASE COUNT          682 a         1645 c         1599 g          569 t
ORIGIN      
        1 gtgcgcgttc gtcccgagcc cgcgcagcac cgcgccgcag atgagcgagg cgtcgatacc
       61 gccggcgcga agcgccatcc atgtcatcgc ggtcgtcgtc gacttgccgt gcgttccggc
      121 gatcgccacg acgcgctgcc ccgtcgtgac ggaccgccag gcgtcctcgc gcttccacac
      181 cgggatcccc tgcgcggtcg cctcgcgcac ctcgacgttg tcgtgaccat aggcgctcga
      241 tcggatgacg atgtccgcgc cgcggacgtt ctccggcgcg aagcgctcgt ggacggcgac
      301 cccgtcgcgc gcgagcgcgt ccgcgagcgg ccacgcgccg gtgtccgagc ccgtgacgtc
      361 gtgcccgcgt gcgaggagga tccgcgccac cgcggacatg ccggcgccgc cgatcccgat
      421 cacgtggaag cgcgtcatgc gcagcctccg agcgccagga tctccgcggc catcaccttc
      481 gatgcatcgc gccgcccggc gttcttcgcc gcctgcgtca tgcggccaaa gcgcgtcgcg
      541 tcgttgagca ggcccgtgac ctgcgcggtg agacggtccg gatccagctc gctctcgcga
      601 acgatcaccg ccgcgcccgc ttcggccacg gcccgtgcgt tcgcctcctg atggccctcc
      661 atcgcagcgc cgaacgggat gaggacgagg ggcgtcccga aggcgagcgg ctccgcgatg
      721 gacgacgcgc ccgctcgtcc gatgaccaga tccgcggccg cgagcaccgc tcccatctcg
      781 tcacgcaggt atggatgcgg caggtaccgc tgacgctgct cctccgtgag cgactgctcg
      841 cgagccttgg ccttggcgat gtgcgcgtcg ccggtgacgt gcagcacgta ggcgctcttc
      901 agcaggcgcg gcagcgccgc gaacaccgtg tcgttcacgc gctccgatcc ctggctgccg
      961 cccgtcacga cgacgagcgg cgcttcgctc ggcacgccca tcttttcgcg cgcgctctca
     1021 cgcgtccagc gcaggagcga cgagcggatc ggcgtccccg tgaccgtcgc gcgtcccgcc
     1081 gggaagtgga cacgggcctg ctcgaacgcg agaccgatct tcgtgcagaa acggccgagc
     1141 gtccggctgg cgcgtcccgg gagcgcgtcc cccgcccaca gatagaccgg cacgcgcagc
     1201 gtgcgtgcgg cgaggatgac ggggaacgcg atcgcgccac cgctcgtcac gcacacgcgc
     1261 gggcggaagg cggcgatgcg cgcgagcgcg tcgacatagg cgaacgggag gcgcagcacc
     1321 gtcccgatga gggcgaatcg cgagtcgggg tcgcggagcg aggcgaccgg cagcgcgtga
     1381 aatgggatgc ccgcctcggg cacgagctta ttctcgagcc cgcgacgacc accgatgaac
     1441 tggagccgca cctcgggatc ggtctcgcgg agcgcctcgg cgatcgcgag cagcgggctg
     1501 gtatgaccgc ccgtcccgcc gccgacgagg agcacgcgac acgctcgggg cgggcccggc
     1561 ctcgcgtcca acattgagct ggatacccac cgcgatgaga cccacgcaca gcgacgagcc
     1621 gccgtacgag atgaacggga gcgtgatgcc cgtcatcggc acgaggctcg cgacgacggc
     1681 catgttcacc cacgcctgga acaccagcca tgtggtgatc cccgcggcga gcagcgcgcc
     1741 cgtcgcgtcc cgcgcccgga gcgcgatggc gaccccgcga taggcgagca cgaagaagag
     1801 gacgatgacg gcgagcgtgc ccgcaaggcc aagctcgtcg cccagcaccg cgaagatcga
     1861 gtccgtgtac ggagccggca ggaagccgaa cttctcacgg ccggccccaa ggccctcgcc
     1921 gaacaggccg ccgagcgcaa ggccgtagag cgactggatg gtctgaaagc cgtcgccgcg
     1981 gggatcgcgc cacggatcga ggaaggtcgt gatgcgctgg agacggtacg gctgctcgat
     2041 cgcgaccgcg aggacgacga cggcgacgag cgcggcgagc gccccgaaga ccttcacgcg
     2101 cgcgcccgcc acgaagtaca tggacatggc gatcgcgacg atcacgatgg ccgtgcccag
     2161 gtcgggctcg cggacgacga gcccggccag gacgagcgtc acaccgaaga gcggcagcgc
     2221 gacgccgggc gtcgcgatgc ggtcgcggcg gcccccgagc cacaccgccg cgtacacgat
     2281 gagcgcgagc ttcgcgagct cggcgggctg gaggcccacg ggaccgagac gcagccagcg
     2341 gcaggcgccg cccgcgcaga cacccaggcc gggcacgagg accagcacga ggagcgcgac
     2401 gacgaggagc acgagcggga ccgcgagcac gcgatagcgg tggtagtcga agcgcatcgc
     2461 cgccaccatc ccgacgagtc cgatcgccgc ccagatcgcc tgccaggtga ggtagtagcg
     2521 cgggtcatcg agcgtatcga gagcccgtat gccgctcgcg ctgtagacca tcgcgatgcc
     2581 gagcagggtc agcgcgagca cgacgaccac gagcgtgagg tcgaacgagg tcgtcctggg
     2641 cagatgcagc ttcatgcgcc cgtcccgctc gcgagctgcc gcacgagcgc cacgaatcga
     2701 tcgccgcggt cgtcggccga cgtgaacgcg tcgacggtct tctggtgcgt gatgtcctcg
     2761 cgggcggcgc aggccggcga gagcaggacg acgtcgccgg tctgcgcgag ctcacgcgcc
     2821 gcggcgatcg cgtccggcag gctcgctgcc cggcgcacgg gcagcctgcc cgcaccgggt
     2881 ccgggcgccg cggcgtcgag gctggcggcg atgtcgtcgg aggccggacc aaggagcacc
     2941 gcagcgcgtg cgcgtcgcac gagcgcgcgc ccgagctcgc cgaactccac ccccttcgag
     3001 acgccgccca ggatcacgac cggcctccgg tcgaacgcct cgagcgcggc cagcgtggct
     3061 gccggcgtcg tggccgcgga gtcgttcacc cacagcacgc cgtcgatctc cgcgacacgc
     3121 tgcagacggt gcggcactcc ctcgaacgcc cgcagggccg cggcgatctc gcccgggggt
     3181 acacccatgg cctggccggc gatggccgcc gccagcgcgt tcgcgatgtt gtgccgtccg
     3241 acgacgcgca gctcgtccgc ggcgagcagc gccgtctcgc gcggaccgtc gacgaggacg
     3301 agctgccccg cggcgcccag gtgcgcgccg cgcttcggcc gcaggccgag cgagtacgcg
     3361 cggacctcgg atgcgctgcc ggtgtgcatc gccacgacga cggggtcgtc gaggttcagc
     3421 accgcgatgt ccttcgagcc ctgccaggcg aggatgttcc gcttcgccgc gatgtagttc
     3481 tcccgcgtgc cgtggtggtc gagatgatcc tcgctgacgt tcgtaatgac cgcgacatgc
     3541 gggctcgtgc cgagcgtctc gagctggaag ctcgagagct cgagcaggac cacgtcgctc
     3601 ctcgtgatgc cgtcgagctc ctggatgatc gagcgcccga tgttcccccc gctgatcacg
     3661 cgccgctgac cacgcctgag gatgtcctcg atcatcgtgg tggtggtcgt cttgcccttg
     3721 gtccccgtga cacccacgat gggtgctggg cacagccgga agaagaggcc gatctccgtc
     3781 aggaccggta tgtcgcgctg cagtgcccgc aggatcgtcg ccgagcgggg ccggatgccg
     3841 gggacgatga agacgaagtc cggatcggcg agggcgctgt cgtcgctcga gggcccgaag
     3901 acgaactcga cgggcgtgtc gccgagccgc gcgatgccct ccgccctctc cgcacggggc
     3961 gcggcatcgc tgatggtcac gcgcgcgccg caggcgacga ggaactgcgc gagccccgcg
     4021 ctggtgcggc ccttccccag cccgacgatc gtcacgcgct tcccgcgcag atcgtcccgc
     4081 gtgatcgggg cgtcgaagac caccgtcacg aggcgagccg cccgatgaag cccagttgcg
     4141 tgccgtactt cccgtcgggg ccgcgcccgc ggtacgacca cacgtcggcg ccgcccgagc
     4201 gcgtgcacac acccgccgtc tcgatgcgca cgacgccggc ggcgcggagc tgcgcggcgt
     4261 tggcttccca gaggtccatg acggcgcggc cgtccacgcg gcggatcgcg ttgccaccga
     4321 tgcgctcggc gacgagcgcg gcgcgcgcgt cgtcgatcgc gtagcagcac ggcccgatcg
     4381 agggaccgat cgcggcgacg agtcgctcgc gcgccgcgcc acccgccacc atggcctcga
     4441 tgagcgcggt cgcgacctta tggctcgtgc cctcccaacc ggcatgcgcg ccgcc
//
LOCUS       DASTZR010000389         2163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_852822, whole genome shotgun sequence.
ACCESSION   DASTZR010000389 DASTZR010000000
VERSION     DASTZR010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852822"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..729
                     /locus_tag="VFV20_08730"
     CDS             91..729
                     /locus_tag="VFV20_08730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU5288473.1"
                     /translation="MRGGDLVLGFASLRDTSVIGASRAPDGRGAIPDAQLVALARESP
                     AEAIEVLYGAYKARIYTFLLRLLSDPELADDVTQDTFTKAYQSLGTLTNEHRVLSWLY
                     RIANNAAIDHLRRRKRISWLRVGGILGGPDEPTVRDANDALPEKDHVRSVLAGMPVEQ
                     ATALLLHALEGYSYKEIAEIQGSTLPAVRSRIARARVAFKMAYETKDTPGSS"
     gene            735..>2163
                     /locus_tag="VFV20_08735"
     CDS             735..>2163
                     /locus_tag="VFV20_08735"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEU5288474.1"
                     /translation="MGNPKQPHAPFQELSALADGELEPIQAAAVQAHVSQCATCASVL
                     DTFVSIDSELQQPPVLACESALTLLSARHDGEADPAEATVAERHLAACAACQARVAAW
                     SRFDAALHSLPAGVPSQRTDAAIAALGRAPQRRARRPVGAALRGAAALATAVALAVAG
                     LSRGGGPVTFTPSLASNDRLLVATAQQIVLNPATSTLYVIDQAPGAIRAQDATTNALK
                     KLIMVGGRPTALALDPLANKILVLDSAEKRVTEIDGASNTVVGSTTMDVAGTPTSIRV
                     DPATSKIIVTSVQPSAAPSHGGTVSVLDSGTKKVEAIRDVAIAPMLAVVDPAGARVAL
                     VSGNETSVVDEGYRVLARLPGGVAAAFARGTGVIAVLSAAGDNAVVSFAGDNAPASLS
                     LSGRPRAMVALPDGGFLVLVEVGGHGRVVRVGADGKQSANVELAISGRDLVYDEANGR
                     FSVADGGIVSSAAVPGTAVAVASPSP"
BASE COUNT          308 a          827 c          743 g          285 t
ORIGIN      
        1 gaaggggtcc caggaattca acgccgttct gaagcggggt tgacggtcca gccgttgagc
       61 gccgcttaac ggaccagtac gctcaccgag atgaggggcg gagacctggt gctgggattc
      121 gcatcgctgc gtgacacgag cgtgatcggc gcgtcgcgcg cgcccgacgg acgcggcgcg
      181 atccccgacg cgcagctcgt cgcgctcgcg cgtgagtcgc cggccgaggc gatcgaggtg
      241 ctctacggcg cgtacaaggc gcgcatctac acgttcctgc tgcgcctgct ctccgatccg
      301 gagctcgcgg acgacgtcac gcaggacacc ttcacgaagg cctaccagtc gctgggcacg
      361 ctcacgaacg agcatcgcgt gctgtcctgg ctctaccgga tcgcgaacaa cgccgcgatc
      421 gaccatctgc gccgcaggaa gcgcatctcg tggctgcggg tcggcggcat cctcggcggc
      481 cccgacgagc cgacggtgcg cgacgccaac gacgcgctcc cggagaagga ccacgtgcgc
      541 agcgtcctcg ccggcatgcc cgtcgagcag gcgacggcgc tcctcctcca cgcgctcgag
      601 gggtactcct acaaggagat cgccgagatc cagggatcca ccctccccgc ggtgcgaagc
      661 cggatcgcac gggcccgcgt ggcgttcaag atggcttacg agacaaagga cacgcccggc
      721 tcgtcttagg ggcagtgggg aaccccaagc agccgcacgc gcccttccag gagctgtccg
      781 cgctcgccga cggcgagctc gagccgatcc aggccgccgc ggtgcaggcg cacgtgtcgc
      841 aatgtgcgac atgcgcgtca gttctggaca cgttcgtgtc catcgacagc gagctccagc
      901 agcccccggt cctggcgtgc gagagcgctc tcacgctcct ctcggcgcgc cacgacggcg
      961 aggccgaccc cgccgaagcc accgtcgccg agcgccacct cgcggcatgc gccgcctgcc
     1021 aggcgcgcgt cgccgcgtgg tcgcgcttcg acgccgcgct gcacagcctc cccgcgggcg
     1081 tcccctcgca gcgcaccgat gccgccatcg ccgccctcgg ccgcgcccca cagcggcgcg
     1141 cgcggcgtcc cgtcggcgct gcgctgcgcg gagcggccgc cctcgcgacc gccgtcgcgc
     1201 tcgccgtcgc gggcctgtcg cgcggtggcg ggccggtcac gttcacgccg tcgctcgcca
     1261 gcaacgaccg gctcctcgtc gcgaccgcgc agcagatcgt cctcaacccc gccacgagca
     1321 cgctctacgt catcgaccag gcgcctggcg cgatccgcgc gcaggacgcg acgacgaacg
     1381 ccctcaagaa gctgatcatg gtcggcggcc gtcccacggc cctcgcgctc gacccgctcg
     1441 cgaacaagat cctcgtgctc gactccgcgg agaagcgcgt gaccgagatc gacggtgcga
     1501 gcaacaccgt cgtcggctcg acgaccatgg acgtcgccgg gacgccgacg agcatccgcg
     1561 tcgatccggc gacgagcaag atcatcgtca cgtccgtcca gccgtccgcg gcgccgagcc
     1621 acggcggcac ggtctcggtc ctcgacagcg gcacgaagaa ggtcgaggcg atccgcgacg
     1681 tcgcgatcgc gccgatgctc gcggtcgtcg atccggccgg cgcgcgcgtc gcgctcgtct
     1741 cgggcaacga gaccagcgtg gtggacgagg gttaccgcgt cctcgcgcgg ctccccggcg
     1801 gcgtcgccgc ggcgttcgcg cgcggcaccg gcgtcatcgc ggtgctctcc gccgcgggcg
     1861 acaacgcggt cgtctccttc gcgggcgaca acgcccccgc atcgctctcg ctcagcggcc
     1921 gcccgcgcgc gatggtcgcc ctgcccgacg gcgggttcct cgtcctcgtc gaggtcggtg
     1981 gtcacggacg cgtcgtgcgc gtgggcgccg acggaaagca gtccgcgaac gtggagctcg
     2041 ccatcagcgg ccgcgacctc gtgtacgacg aggccaacgg acgcttcagc gtggcggacg
     2101 gcgggatcgt gagctcggcc gcggtgccgg gcacggccgt cgccgtcgcg tcgccctcgc
     2161 cca
//
LOCUS       DASTZR010000390         2572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_517833, whole genome shotgun sequence.
ACCESSION   DASTZR010000390 DASTZR010000000
VERSION     DASTZR010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517833"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="VFV20_08740"
     CDS             complement(<1..1128)
                     /locus_tag="VFV20_08740"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEU5288475.1"
                     /translation="MIPLFVLTALGVALTAGFGLGLWLLLARTAGLTTGPLAWTALVQ
                     THGTIQLFGFSAMFLMGVGLHVLPRFRGAPAAPRALALVAYGATAASIMLRAVAQPLP
                     DLPARAIVLPLSGALLVAGTTAFAAAALRALRGGTNPHRPDELVMAAGVLAAPLAALL
                     VASAVVAAPLVIAQEADDRGIWAMLLGCLATAIFGVWARLAPGFIATPPARPRPLIAG
                     AAVWLLGAALVVAGVPFASVALVAGLGLIVRALGLFGATIARQPLVAHARLTRIAARS
                     AFAWGAAGALMLVAYDVRALAGGGPASYVEVSAARHAFALGLVTLMIYGVAARALPSF
                     LNRRSWSLGLQTATLILANLGVALRVVPQMLGANDALANAVV"
     gene            complement(1125..2264)
                     /locus_tag="VFV20_08745"
     CDS             complement(1125..2264)
                     /locus_tag="VFV20_08745"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5288476.1"
                     /translation="MTETEPAGRFGDLEIHPDVARAIDELGFMAPTPVQAQAIPVLRA
                     GRDLLAQAQTGTGKTAAFAIPIIERVDPSEKRPQALIVVPTRELAVQVTREFASLGKY
                     RRAHEVAIYGGVPYGPQERALARGAQIVVGTPGRLLDHIERGTLKLDGVRIVILDEGD
                     RLLDMGFAPDVRKLLRLVPKQRQTALFSATLTGEIKDLAYRFTNEPTQIAIDPERRTQ
                     ESVEQVYVEVLEEDKPRAIEELLRRWETEQVLVFRHTKRGVDRLLDDLKRRGHAVAAI
                     HGDMTQRERERTLDDFRSGDVKILVATNLAARGLHIEDISHVVNYDLPEDAETFTHRI
                     GRTGRAGETGVAVTFVAEWDFDEFERLRTKAGVPFRREVLELYGR"
     gene            complement(2261..>2572)
                     /gene="deoC"
                     /locus_tag="VFV20_08750"
     CDS             complement(2261..>2572)
                     /gene="deoC"
                     /locus_tag="VFV20_08750"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013467814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEU5288477.1"
                     /translation="ALVKVILECAYLDDAQKAIAAQIAVAAEADYVKTSTGFGPEGAT
                     VEDVRLLRQVVGDRVGVKAAGGIRTLDDALAMLEAGASRLGTSSTTAILDELRQRRGE
                     A"
BASE COUNT          379 a          909 c          944 g          340 t
ORIGIN      
        1 gacgacggcg ttcgcgaggg catcgttcgc accaagcatc tgcggcacga cgcgaagcgc
       61 cacgcccagg ttcgcgagga tgagtgtcgc cgtctgcagg ccgaggctcc agctccgtcg
      121 gttgaggaac gaggggagcg cgcgcgccgc gacgccgtag atcatgagcg tcaccagtcc
      181 cagcgcgaag gcgtgacgtg cggccgagac ctcgacgtac gaggccgggc cgccccccgc
      241 cagcgcccga acgtcgtatg cgaccagcat cagcgcgccg gccgctcccc acgcgaacgc
      301 ggaacgtgcc gcgatgcgtg tgaggcgtgc gtgtgcgacc agcggttgcc gcgcgatcgt
      361 ggcgccgaac agaccgaggg cgcgcacgat gagccccagt ccggcgacga gcgccaccga
      421 ggcgaacggg acgccggcga cgaccagcgc cgcgcccagg agccagaccg cggcgccggc
      481 gatgagcggc cgcggccgcg cgggcggggt cgcgatgaaa cccggcgcga gccgtgccca
      541 cacgccgaag atcgcggtcg cgagacagcc gaggagcatg gcccagatgc cgcgatcgtc
      601 ggcctcctgc gcgatcacca ggggtgccgc gacgaccgcg ctcgcgacca gcagggccgc
      661 gagcggcgca gcgaggacgc ccgcggccat cacgagctcg tcgggccggt gcgggttggt
      721 gccgccccgc agcgcacgca gagccgccgc cgcgaacgcc gtcgtgccgg cgacgaggag
      781 cgcgcccgag agggggagca cgatggcgcg cgcgggcagg tccggcagcg gctgcgcgac
      841 ggcgcgcagc atgatcgacg ccgcggtcgc gccgtacgcg acgagcgcga gcgctcgcgg
      901 cgcggccggt gcgccgcgaa agcgcggcag cacgtgcagc ccgacgccca tgaggaacat
      961 cgcggagaac ccgaagagct ggatggtccc gtgcgtctgc acgagcgcgg tccacgcgag
     1021 cggtcccgtc gtgaggccgg cggtacgcgc gaggagcagc cagagaccga gtccgaatcc
     1081 cgcggtgagg gcgacgccga gcgccgtcag gacgaagagc gggatcatcg cccgtagagc
     1141 tcgagcacct cgcgccggaa cgggaccccg gccttcgtcc tgagccgctc gaactcgtcg
     1201 aagtcccatt ccgcgacgaa ggtcacggcg acgccggtct cacccgcgcg gccggtgcga
     1261 ccgatgcgat gcgtgaacgt ctcggcgtcc tccggcaggt cgtagttgac gacgtggctg
     1321 atgtcctcga tgtggaggcc acgcgcggcg aggttcgtgg cgacgaggat cttgacgtcg
     1381 cccgagcgga agtcgtcgag cgtgcgctcg cgctcgcgct gcgtcatgtc gccgtggatg
     1441 gcggcgaccg catgaccacg tcgcttgaga tcgtccagca ggcgatccac gccgcgcttc
     1501 gtgtgtcgga acacgagcac ctgctcggtc tcccagcggc gcaggagctc ctcgatggcg
     1561 cggggtttgt cctcctcgag cacctcgacg tacacctgct cgacgctctc ctgcgtgcgg
     1621 cgctcgggat cgatggcgat ctgcgtgggt tcgttggtga agcggtacgc gagatccttg
     1681 atctcaccgg tgagcgtcgc cgagaagagc gcggtctgtc gctgcttcgg cacgagccgc
     1741 agcagctttc gcacgtctgg tgcgaacccc atgtcgagca gccggtcgcc ttcgtcgagg
     1801 atcacgatgc gcacgccgtc gagcttcagc gtgccgcgct cgatgtggtc gaggagccga
     1861 cccggtgttc cgaccacgat ctgcgcgccg cgcgcgagcg cgcgctcctg cggtccgtag
     1921 ggcacgccgc cgtagatcgc gacctcgtgc gcgcgacggt acttgccgag ggacgcgaac
     1981 tcgcgcgtga cctggaccgc gagctcgcgt gtgggcacga cgatgagcgc ctgtgggcgc
     2041 ttctcgctcg gatcgacacg ctcgatgatg gggatggcga acgcggcggt cttgccggtg
     2101 cccgtctgcg cctgcgcgag caggtcgcgg ccggcccgca ggaccgggat ggcctgcgcc
     2161 tgcaccggcg tcggtgccat gaagcccagc tcgtcgatcg cgcgcgcgac gtcgggatgg
     2221 atctcgagat cgccgaagcg ccccgccggc tccgtctccg tcacgcctcg ccgcgccgtt
     2281 gtcggagctc gtcgaggatc gccgtcgtcg acgacgtccc gaggcggctc gcgcccgcct
     2341 cgagcatcgc gagcgcgtcg tcgagagtgc gtatcccgcc ggcggccttg acgccgaccc
     2401 tgtcaccgac gacctgtcgc agcagccgca catcctcgac cgtcgcgccc tccgggccga
     2461 agccggtgga cgtcttcacg tagtcggcct ccgccgcgac ggcgatctgc gcggcgatcg
     2521 ccttctgcgc gtcgtcgagg tacgcgcact cgaggatgac cttcacgaga gc
//
LOCUS       DASTZR010000391         2060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1014044, whole genome shotgun sequence.
ACCESSION   DASTZR010000391 DASTZR010000000
VERSION     DASTZR010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014044"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1595)
                     /locus_tag="VFV20_08755"
     CDS             complement(<1..1595)
                     /locus_tag="VFV20_08755"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEU5288478.1"
                     /translation="MAGRRGNGGPEWGNIDWSNFDLRQLGGGGRGAARTPRPPTRRGI
                     ALTVVVVVLLLVPLVLSPLVSFLTDLLWFRSLGFEDVYLRRYTAGFWAFVAFGLLFFV
                     LALPNFYLAMRPQVPRVVVDAKRARPSALAATLRWMWVLLLPSFFFGIAGGDQWDPLL
                     RWLNAVPFGVTDPVFGRDIGFYFFTLPMLDFARGWAIAAVLVIAAGVVLVYALRGVIG
                     IATDAFSRGDIAVAGRTALALARPARAHLSVLGGVFLLLVAFGYVIDQWDLLFRQETV
                     LTGAGYTSMNARLPALTILAVLVGIAALACFANAFARTLWVLGGAIVLWFVASILVLG
                     VYPGLIENFIVRPDQFNKERPYLERNIAATRAAYRLDNVEESLFNVAETPTLSDARRD
                     LSDTDAIRLWDYRPLLSTFDIQALRQYYIFNDVDVDRYQIGGREVPVMLSARELSTSR
                     LPQNTWVNRHLVFTHGYGAVLTAVGSVGPEGRPELLVKDIPPQGEPKIDQPRIYYGEL
                     TRNYAIVGTTQDEFDYASERDVTT"
     gene            complement(1627..>2060)
                     /locus_tag="VFV20_08760"
     CDS             complement(1627..>2060)
                     /locus_tag="VFV20_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288479.1"
                     /translation="VGTCVGVAVGACVGAAVGAVVGCAVCCCVGDGVADAVAVAVGDA
                     LTLGLALALGDGLAATTVVGVGAALVAAGRVTDGCPSGVGFPLSSLVCCPWTHDARNS
                     PAVNADTSLNKFELPPFLDPPSGTRRGLAAIVSTACSGERP"
BASE COUNT          356 a          703 c          748 g          253 t
ORIGIN      
        1 gtcgtcacgt cgcgctcgct cgcgtagtcg aactcgtcct gcgtcgtccc gacgatcgcg
       61 tagttgcgcg tcagctcgcc gtagtagatc cgcggctggt cgatcttcgg ctcgccctgc
      121 ggcgggatgt ccttcaccag cagctccggc cggccctccg gtccgacgct gccgacggcg
      181 gtgaggacgg cgccgtagcc gtgggtgaac acgaggtgac ggttgaccca ggtgttctgc
      241 ggcagacgcg acgtcgagag ctcgcgcgcg gagagcatga ccggcacctc gcgaccgccg
      301 atctggtacc gatccacgtc gacgtcgttg aagatgtagt actggcgcag cgcctggatg
      361 tcgaaggtcg agagcagcgg ccggtagtcc cagaggcgga tcgcgtcggt gtcgctcaga
      421 tcgcggcgcg cgtcggagag cgtcggcgtc tccgcgacgt tgaagagcga ctcctcgacg
      481 ttgtcgagcc ggtaggccgc tctcgtcgcg gcgatgttgc gttcgaggta cggccgctcc
      541 ttgttgaact ggtcaggccg cacgatgaag ttctcgatga gccccgggta gacgcccagc
      601 acgaggatgc tggcgacgaa ccagaggacg atcgcgccgc cgagcaccca gagcgtgcgc
      661 gcgaacgcgt tggcgaagca cgcgagcgcg gcgatcccga cgaggaccgc gaggatggtc
      721 agcgcgggaa ggcgcgcgtt catgctggtg tacccggctc cggtgaggac cgtctcctga
      781 cggaagagca ggtcccactg gtcgatcacg tagccgaaag cgacgagcag gaggaacacc
      841 ccaccgagca ccgagaggtg cgcgcgcgcc ggacgcgcga gcgcgagggc ggtgcggccg
      901 gccacggcga tgtcgccgcg gctgaacgcg tcggtcgcga tgccgatgac gccgcgcagc
      961 gcgtacacga gcacgacccc cgccgcgatg acgagcaccg cggcgatggc ccagccgcgg
     1021 gcgaagtcga gcatcggcag cgtgaagaag tagaagccga tgtcgcggcc gaagaccggg
     1081 tcggtcacgc cgaacgggac ggcgttcagc caccgcagca gcgggtccca ctggtcgccg
     1141 cccgcgatcc cgaagaagaa cgaggggagg agcagcaccc acatccagcg cagcgtcgcc
     1201 gcgagcgccg acggccgcgc gcgcttcgca tcgacgacga cgcgcgggac ctgcgggcgc
     1261 atcgcgaggt agaagttcgg cagcgcgagg acgaagaaga gcaggccgaa ggcgacgaac
     1321 gcccagaagc ccgcggtgta gcggcgcagg tacacgtcct cgaagccgag gctccggaac
     1381 cagagcaggt cggtgaggaa cgagacgagc gggctgagca cgagcggcac gaggaggagc
     1441 acgacgacga cgaccgtgag cgcgatgccg cgccgcgtcg gtggtcgcgg cgtgcgggcc
     1501 gcgccgcggc cgccgccgcc gagctggcgc aggtcgaagt tggaccagtc gatgttgccc
     1561 cactcggggc cgccgttccc ccgccttcct gccatcagcg tgagtatgtg gccgatcagt
     1621 tcggtctcac ggccgctcac cagagcaagc cgtcgataca atggccgcca ggccgcgtcg
     1681 cgtgccgctt ggtggatcga ggaacggggg aagctcgaac ttgttcagac tggtgtccgc
     1741 attgaccgct gggctgttcc tcgcatcgtg cgtccaaggg cagcagacca gactcgagag
     1801 cgggaacccg acgccgctcg ggcagccatc ggtcaccctt ccggcggcga cgagcgcggc
     1861 gcccacgccg acgaccgtcg tcgcggcgag cccgtcaccg agcgcgagcg cgagcccgag
     1921 cgtcagcgcg tcgccgaccg cgaccgcgac ggcgtccgcg acgccatcgc caacgcagca
     1981 gcagacggcg cagccgacca ccgcgcccac tgccgcaccg acgcaggcgc cgaccgccac
     2041 gcccacgcag gtccccactc
//
LOCUS       DASTZR010000392         3903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_803434, whole genome shotgun sequence.
ACCESSION   DASTZR010000392 DASTZR010000000
VERSION     DASTZR010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803434"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VFV20_08765"
     CDS             <1..454
                     /locus_tag="VFV20_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288480.1"
                     /translation="ASCPGCADAMRDLGATAVALERAYAPLRARTVNLSAARVRLAVR
                     TPARPSRIVRYARLTAKLNELAVAAAVMVFAVIGTVPSTVVIVEDEPTAATRFQLMQG
                     FEEPRSEPYVSQRIGRYLLHDTLLDVRVVPLNDAWPSAPQQVMPGEPY"
     gene            571..2043
                     /locus_tag="VFV20_08770"
     CDS             571..2043
                     /locus_tag="VFV20_08770"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5288481.1"
                     /translation="MGEPLSAMAEDAGGRVIRAAVTETLVGRDREGAFVARLAVAVPT
                     MANGGLRLERDQDGIGERLVATFELREDARWQDGAPITAADIRFAFDEDRLAPAGSER
                     RWMADRVERMDEISARAVRVVYRRGERWELYALAPHVLPAHLLAGATAERRAAYDRAP
                     VHAGPFAVAAWVRGYGVTLSAFRDHVGGPPHLGRIEVRFFRDPLTLADALLRGDVDVV
                     PSPAFDVDMIGTLERLADRRGLQAQYTPAMSVEMLRLGARGLAADPSVRRALLLAVDR
                     GGMADTLFAGRVRVPRSYLVPPSPAAREALPPLRADRAEAAQLLAAAGFSRGAIGVLE
                     RGGERLVLTLQVGGASPARIETARRIASDLGSLGIALNVLQRTLEDVGAAVSSGDFEL
                     ALLPERGDDVARASERYRGRVDPWFDLLVDAALRSANESDRLDAYAELQRLWSMSLAG
                     IPIYQDLLVNVAPRDLVGVQPVAHGDPLTWNVAEWSFARE"
     gene            2237..>3903
                     /locus_tag="VFV20_08775"
     CDS             2237..>3903
                     /locus_tag="VFV20_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5288482.1"
                     /translation="MTKRRNGLSVFALLVIASLIATACSGTPGTSSPTGSGAASDVDP
                     NGELTLNTSTEPNTIDPQKSSFVDEIGQAAMVFEGLMTFDSQTTKTIPAAATDQPKWD
                     ASKLTYTYTVKDGLKYSDGTPLTAKNFEYAFKRGCDPTQAGDYAFVLFVIVGCEEWNS
                     ADTKKATPAELTALKDKVGIKATDDKTLVFTLKEPASYFIAISALWIGWPVRESDVTK
                     GGDKWTEPATYIGNGPFKLVEWKHNEKLVYEPNPNWRKGTVKIKKLTFVMIQEANVAF
                     AAYRNNELDMTSVTAGDLRTVEGDPDLSKQVVDTKGSCSYYYGFNVRRPPFDDVNVRM
                     AFARSFDREAFIKDVIKIGKAADGGFIPPGFPGYDNADKVQSFDPKAAKALLDKAAPA
                     SKAGLADTKFTYGATALGKTYAEWIQAQWKTNLGVEVQLDPVPSNGFSALFRKGKDSP
                     QLFSLGWCADFPDEQNWHSTVWRIGGISASRTGYSSKEFDDLTKAADKEADAGKRAET
                     YKKAGQLLSGEAPGAWIRYGASKSVRKPWIKGIIDNPIDYGVPGYFSL"
BASE COUNT          613 a         1371 c         1350 g          569 t
ORIGIN      
        1 cgcgagctgc ccgggctgcg ccgacgcgat gcgggacctc ggcgcgacgg ccgtggcgct
       61 cgagcgggca tacgctccgc tgcgcgcgcg caccgtgaac ctgtccgccg cccgcgtccg
      121 tctcgccgtc cgcacgccgg cgcggccgtc gcgcatcgtg cggtacgcgc gcctcacggc
      181 caagctcaac gagctggccg tcgcggccgc ggtgatggtc ttcgccgtca tcgggacggt
      241 gcccagtacc gtggtcatcg tcgaggacga gccgacggca gcgactcgtt tccagctcat
      301 gcaggggttc gaggagccgc gctccgagcc ctacgtctcg cagcgcatcg gccgctacct
      361 cctccacgac acgctcctcg acgttcgcgt cgtcccgttg aacgacgcct ggccatcggc
      421 gccgcagcag gtgatgcccg gcgagcccta ctgagccggg tcgcgtcctc ggggtccccg
      481 cgcgcgttcc tcgccctcct gctcctggtc gccgcggcga cctcgtgtag cgcgccgacg
      541 cccgcgcgcc gcgacgtggt gctcgggctg gtgggcgaac cgctctccgc gatggcggag
      601 gacgccggcg gccgcgtcat ccgcgccgcg gtcaccgaga cgctggtcgg acgcgaccgc
      661 gagggcgcgt tcgtggcgcg tctcgccgtc gcggtcccca cgatggcgaa cggcggcctg
      721 cgcctcgagc gggaccagga cgggatcggg gagcggctcg tcgcgacgtt cgagcttcgt
      781 gaggacgcgc gctggcagga cggcgcgccg atcaccgcgg cggacatccg cttcgcgttc
      841 gacgaggacc gccttgcccc cgcgggcagc gagcggcgct ggatggcgga ccgcgtcgag
      901 cgcatggacg agatctcggc gcgggcggtc cgcgtcgtgt accggagggg cgagcgctgg
      961 gagctgtacg cgctcgcgcc ccacgtcctt ccggcgcacc tgctcgcggg tgccacggcg
     1021 gagcggcgtg cggcgtacga ccgcgcgccg gtccacgccg gcccattcgc cgtcgcggcg
     1081 tgggtgcgag gctacggcgt gacgctttcg gcctttcgcg accacgtcgg aggaccccca
     1141 catctcggcc ggatcgaggt gcgcttcttc cgcgatcctc tgacgctggc cgacgcgctg
     1201 ctgcgcggcg acgtcgacgt cgtgccgtcg cccgcgttcg acgtggacat gatcgggacc
     1261 ctcgagcggc tcgcggaccg gagagggctg caggcgcagt acacgccggc gatgtcggtg
     1321 gagatgctgc gtcttggagc gcggggcctg gccgccgacc cgagcgttcg ccgcgcgctc
     1381 ctgctggcgg tggatcgcgg tggcatggcc gacacgctct tcgcaggacg cgtgcgcgta
     1441 ccgcggtcgt acctcgtgcc gcccagcccg gccgcgcgcg aggcgcttcc gccgctgagg
     1501 gccgatcgtg cggaggccgc gcagctcctc gcggcagccg gcttctcgcg cggcgcgatc
     1561 ggcgtcctcg agcgtggcgg cgaacgcctc gtcttgacgc tccaggtcgg cggcgcgtcg
     1621 cccgcgcgta tcgagacggc ccgccggatc gcgagcgatc tcggctcgct cggcatcgcc
     1681 ctgaacgtgc tccagcggac gctcgaggac gtcggtgccg cggtgtcgtc tggagacttc
     1741 gagctcgcgc tgctgcccga gcgtggcgac gacgtcgccc gcgcatccga gcgctaccgc
     1801 ggtcgggtcg acccatggtt cgacctcctc gtcgatgcgg cgctgcgctc ggcgaacgag
     1861 tcggaccggc tcgacgcgta cgccgagctg cagcggctgt ggtcgatgtc cctcgccggg
     1921 atcccgatct accaggatct gctggtgaac gtcgcgccac gggacctggt gggcgtccag
     1981 ccggtcgccc acggcgaccc gctcacgtgg aacgtggccg aatggagctt cgcgagggag
     2041 tgagccggcc cggaaaacgg cgagtgaact ggcccgtgcc cacggcggta taagcactga
     2101 tgggctcaat gtgtcaggct gcgcgccgtt tgacgcgtct tggtacagag acccacaatg
     2161 gcaccgattg ctcgcacgct gtcctggggg gccagaagtg cgggcaccca cagcgaatca
     2221 gcaggaggag gagcgcatga ccaagcgccg caatggcctc agcgtgttcg cgttgctcgt
     2281 gatcgcgtcc cttatcgcga cggcatgctc cggaacgccg ggcacgtcgt caccgacagg
     2341 gagcggcgcc gcttcggatg tcgacccgaa cggcgagctc acgctcaata cgagcaccga
     2401 gccgaacacg atcgacccgc agaagtcgtc cttcgtcgac gagatcggac aggctgcgat
     2461 ggtgttcgag ggcctcatga ccttcgactc gcagacgacg aagacgatcc ccgccgcggc
     2521 taccgaccag ccgaagtggg acgccagcaa gctcacctac acgtacacgg tcaaggacgg
     2581 cctcaagtac tcggatggca caccgctcac ggcgaagaac ttcgagtacg ccttcaagcg
     2641 cgggtgcgac ccgacacagg ccggtgacta cgccttcgtc ctcttcgtga tcgtcggttg
     2701 cgaggagtgg aacagcgccg acacgaagaa ggcaacgccc gccgagctca cggccctgaa
     2761 ggacaaggtc gggatcaagg cgaccgacga caagacgctg gtgttcaccc tcaaggagcc
     2821 ggcgtcctac ttcatcgcca tcagcgcgct gtggatcggc tggccggtcc gcgagtccga
     2881 cgtcaccaag ggtggcgaca agtggaccga gcccgcgacc tacatcggca acggcccctt
     2941 caagctcgtc gagtggaagc acaacgagaa gctcgtctac gagccgaacc cgaactggcg
     3001 gaagggcacc gtcaagatca agaagctcac cttcgtcatg atccaggagg cgaacgtcgc
     3061 gttcgccgcg taccgcaaca acgagctcga catgacgagc gtgaccgcgg gtgacctccg
     3121 cacggtcgag ggcgacccgg acctcagcaa gcaggtggtc gacacgaagg gctcgtgctc
     3181 gtactactac ggcttcaacg tccgccgtcc gcccttcgac gacgtgaacg tccgcatggc
     3241 attcgcgcgg tcgttcgatc gcgaggcgtt catcaaggac gtcatcaaga tcggcaaggc
     3301 cgccgacggc ggcttcatcc cgccgggctt ccccggatac gacaacgccg acaaggtcca
     3361 gtcgttcgac ccgaaggccg cgaaggccct cctcgacaag gcggcccctg cctcgaaggc
     3421 tggtctcgcc gacacgaagt tcacgtacgg cgcgaccgca cttggcaaga cgtacgcgga
     3481 gtggatccag gcccagtgga agaccaacct cggtgtcgag gtccagctgg acccggtgcc
     3541 gtccaacggc ttctccgccc tgttccgcaa gggcaaggac tccccgcagc tgttctcgct
     3601 cggctggtgc gctgacttcc cggacgagca gaactggcac tcgaccgtgt ggcggatcgg
     3661 cgggatcagc gccagccgca ccggctactc gagcaaggag ttcgacgacc tgaccaaggc
     3721 cgccgacaag gaggccgatg ccggcaagcg tgccgagacc tacaagaagg cgggccagct
     3781 cctcagcgga gaagctccgg gggcctggat ccgctacggc gcgagcaaga gcgtccgcaa
     3841 gccgtggatc aagggcatca tcgacaaccc gatcgactac ggcgtcccgg gctacttcag
     3901 cct
//
LOCUS       DASTZR010000393         2153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_826314, whole genome shotgun sequence.
ACCESSION   DASTZR010000393 DASTZR010000000
VERSION     DASTZR010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826314"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VFV20_08780"
     CDS             complement(<1..217)
                     /locus_tag="VFV20_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288483.1"
                     /translation="MGRTRSYVLIVAALGAVSVAQAARTLRLEGVDLLMLAILVTLAA
                     VTQRMPVFLFRSSAISISFAAVIASYVL"
     gene            complement(217..1812)
                     /locus_tag="VFV20_08785"
     CDS             complement(217..1812)
                     /locus_tag="VFV20_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU5288484.1"
                     /translation="MGSLRGLLAAALAVAFVASSLGTAWTARTPAFHAYVDPSLRAGE
                     VALVHTTAGGAGALSSKLALAGAFDIETAAAADTVIARLTPDALATISSDPSVTVATR
                     DIAIHATGGGSDRPGYEKDDDKDRTSSGGRQNATSLAAIGAQRAWQKTEGEVTVAVMD
                     TGIAEHPDLPNDKVRARVDFVNDGSQLGDAGGHGTFIAGVIAANGESYKGVAPEADLV
                     SLRVLDANGNGTAVGVVRAFNWLLKNRAHFNIKVLNISWGAPQATSYQNDLLSALVES
                     AWFSGVTVVVAAGNDGPVAGTVTSPGSDPFVVTVGSFADQGTTDLGDDRESVFSSRGP
                     TLDGFVKPDTLAPGQHVLGLRMRGQVYLGADGQPIGTTADRYIHMTGTSASAAFVSGV
                     AALVLAKNARYTPTQVKGAIVASGRAISGSTTRAVDANAALTSMTAVNVGLRPSRVLL
                     LLLAKLHLLKGQGVTWEGVTWEGVTWDGVTWDGVTWDGVTWDGVTWDGVTWESVSWES
                     ISWESVSWETVTWEGVTWEGMIQ"
BASE COUNT          312 a          754 c          758 g          329 t
ORIGIN      
        1 acagcacgta gctggcgatg accgcggcga aggagatcga gatcgccgag gagcggaaca
       61 ggaagaccgg catgcgctgg gtcaccgcgg cgagggtcac caggatcgcg agcatcagca
      121 ggtccacgcc ctccaggcgc agggtccggg cggcctgcgc caccgagacc gcgcccagcg
      181 cggcgacgat gaggacgtac gacctcgtac ggcccatcac tggatcattc cttcccatgt
      241 cacaccctcc cacgtgacgg tctcccagct gacgctctcc cagctgatgg attcccagct
      301 cactgattcc catgtgacgc cgtcccacgt gacgccgtcc caggtgacgc cgtcccaggt
      361 gacgccatcc caggtcacgc cgtcccacgt gacgccttcc cacgtcacgc cttcccacgt
      421 cacgccctgg cccttgagca ggtgcagctt cgcgagcagc aggagcagga cgcgcgatgg
      481 ccgcaggccg acgttcaccg cggtcatgct cgtgagcgcg gcgttcgcgt cgacggcgcg
      541 cgtcgtggat ccgctgatcg cgcggccgct cgcgacgatg gcgcccttca cctgcgtcgg
      601 tgtgtagcgg gcgttcttcg cgagcacgag cgcggcgaca ccgctgacga acgcggccga
      661 cgccgatgtt ccggtcatgt ggatgtagcg gtccgccgtc gtgccgatcg gctgaccgtc
      721 ggcgccgagg tagacctggc ccctcatgcg caggccgagc acgtgctgtc cgggcgcgag
      781 cgtgtcgggc ttcacgaagc cgtcgagcgt cgggccgcgg ctcgagaaga ccgactcgcg
      841 gtcgtcgcca aggtccgtcg tgccctggtc ggcgaacgag ccgacggtca cgacgaacgg
      901 gtcgctgccg ggcgaggtca ccgtgccggc gacgggaccg tcgttacccg cggcaacgac
      961 gaccgtcacg cccgagaacc acgcgctctc gacgagcgcg gacagcaggt cgttctggta
     1021 ggacgtcgcc tgcggcgcgc cccacgagat gttgagcacc ttgatgttga agtgcgcgcg
     1081 attcttcagc agccagttga aggcgcgcac cacgccgacc gcggtcccgt tgccgttcgc
     1141 gtcgagaacg cgcagcgaga cgagatcggc ctcgggggcg acacccttgt agctctcgcc
     1201 gttggccgcg atgacaccgg cgatgaaggt gccgtggccg cccgcgtcgc cgagctggct
     1261 gccgtcgttc acgaagtcga cgcgcgcgcg caccttgtcg ttcggcaggt ccgggtgctc
     1321 ggcgatgccg gtgtccatga cggccaccgt gacctcgccc tcggtcttct gccacgcacg
     1381 ctgagcgccg atcgccgcga ggcttgtcgc gttctggcga ccgccgctcg acgtcctgtc
     1441 cttgtcgtcg tccttctcgt accccggtcg atcactgccg ccaccggtcg cgtggatcgc
     1501 gatgtcgcgc gtcgcgacgg tcaccgatgg atcgctgctg atggtcgcga gcgcatctgg
     1561 cgtgaggcga gcgatgaccg tgtccgcggc ggcggccgtt tcgatgtcga aggcgccggc
     1621 cagcgcgagc ttgctggaga gcgcgccggc gccgcctgcc gtcgtgtgca cgagggcgac
     1681 ttcaccggcg cgcagggagg ggtcgacgta ggcgtggaac gcgggagtgc gggcggtcca
     1741 cgccgtgccg agcgacgacg cgacgaaggc gacggcgagc gccgccgcaa gaagaccccg
     1801 gagactcccc atgcccctca tgtgtctggg tgtgttgtgc gcttcggggg agggacccac
     1861 atcccccgca cgggtggagt gggtgacgct ctgagaccca ccagatacgc ggtacgtcaa
     1921 ctgacggagt ccgcgtgcgc cgcgtcgcgt agggcgcgcg ccgacgcgcc ctgagcttga
     1981 gggcgcgctg agctcagcgc gtgcgaaccg ccgccagcgc ctgctggaag acctgctgtg
     2041 tctcgtcgcc gccgccgtgc agcagcttgc ggcgctcgcc cacgacggtg gcgatgaacg
     2101 cctcgacgac gaccgggttg aactgcgtgc cggcgttggc gcgcagctcg cgc
//
LOCUS       DASTZR010000394         3409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_909581, whole genome shotgun sequence.
ACCESSION   DASTZR010000394 DASTZR010000000
VERSION     DASTZR010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909581"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VFV20_08790"
     CDS             complement(<1..537)
                     /locus_tag="VFV20_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5288485.1"
                     /translation="MLLEQFFDAGLGHASYLVADPDEGVAFLVDPDRHADRYLEAAAR
                     LGVLITHSFETHVHNDYVAGSVSFAQVRPISVVTGAAAQVSYPHIALADGETIDVGAL
                     RVRCLATPGHTPEHVSFLVADLRRDDDAQLLFSGGALLVGHIARVDLLGPELGERLAR
                     DAYDTLRDRLLALADYVAV"
     gene            complement(556..1326)
                     /locus_tag="VFV20_08795"
     CDS             complement(556..1326)
                     /locus_tag="VFV20_08795"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEU5288486.1"
                     /translation="MRPTTEPRHLDRAWLEQHLAEERRESDMLGEIREALFGMQDGIV
                     STLALASAVSGATGDRYPILVAGIASALAGVFSMAAGEYLSSKSQREIYVAQIENERE
                     EVRDRPDEAEAEVALMLEHEGLGEAAARRVAAELAREPSVLLKTMVEKELGLVIEEGH
                     GALQGAVIMGVSFGTAALVPLIPYMILPVSTAVWGSMVFSGVVMFGIGVLKSRWTRRN
                     PIVSGIEIVVLATVAGVAGFLFGTVLPALLGVAGVVAM"
     gene            complement(1335..2576)
                     /locus_tag="VFV20_08800"
     CDS             complement(1335..2576)
                     /locus_tag="VFV20_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5288487.1"
                     /translation="MDLSWLSADGRRLLLTRILRTFAYGYLAVVLAVYLARLGYSELQ
                     VGFVLSAAVAGSAAMTVVWTLIADRVGRRRTVMVLSALMALGGTLFALASSFDLLMLA
                     AFTGTISATSSEVGAFVTVEQAILPQTAPAEQRTWLFSLYAFVANAAQAVGALFAGVA
                     VGLAAAAGLGGADAYRPLFVLYAIVGLANLAIFWTLSDRVEIARVEGERRLLGIHRSR
                     GIVARLSALFAIDAFAGGLVVQSIVAYWFDLRWGFTPQELGVLFFGVNILSGLSLLVA
                     GRLAMRIGLINTMVFTHIPSNVLLVLVPLMPTGALAAAMFLARMSISQMDVPTRQSYT
                     MAVVDPDERVAAAGLTNVARGTATAFAPALTGYAFSVAALGVPFFAAGALKIAYDLLV
                     YRSFRGVRPPEERRDARATVR"
     gene            complement(2579..2896)
                     /locus_tag="VFV20_08805"
     CDS             complement(2579..2896)
                     /locus_tag="VFV20_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU5288488.1"
                     /translation="MRPEELSKDRAAHHLLDVREDDEWRAGHIDGAQHIPLGQLGDRL
                     AELPAGRSIVAVCRSGSRSAAAVRGLKQLGYAAENLDGGVTAWSRAGLPLVDEAGRAG
                     RVI"
     gene            2926..3291
                     /locus_tag="VFV20_08810"
     CDS             2926..3291
                     /locus_tag="VFV20_08810"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEU5288489.1"
                     /translation="MEKSKARERLRTLREEFTATVDAIRTRLTDSQRESGGDISLADQ
                     HPADVATETADRELDTSRGAMFEARIRQIDDALERLEAGTYGTCLVCGQPIPDERLEA
                     IPDTPYCVKDAEKEQARAS"
BASE COUNT          587 a         1201 c         1204 g          417 t
ORIGIN      
        1 gacggcgacg taatccgcga gcgcgagcag ccggtctcgc agggtgtcgt acgcgtcccg
       61 tgcgagccgc tcgccgagct ccggtcccag cagatccaca cgcgcgatgt ggccgaccag
      121 gagcgcaccg ccgctgaaga ggagctgcgc gtcgtcatcg cgacgcaggt ccgccacgag
      181 aaaggagacg tgctcgggcg tgtgcccggg cgtcgcgaga cagcggaccc gcagggcgcc
      241 gacgtcgatc gtctcgccat cggccagcgc gatgtgcgga taggagacct gcgccgcggc
      301 gcccgtgacg acggagatgg gccggacctg ggcgaacgag accgatccgg cgacgtagtc
      361 gttgtggacg tgcgtctcga acgaatgcgt gatgaggacg ccgagccgcg ctgcggcctc
      421 cagataccgg tcggcgtggc ggtccgggtc caccaggaag gcgacaccct cgtccgggtc
      481 cgcgacgagg taggacgcgt gacccaggcc ggcatcgaag aattgctcca gcagcatcga
      541 gtgaatgcta ggcggtcaca tcgccacgac acccgcgacg ccgaggagcg cgggcagcac
      601 cgtgccgaac aggaacccgg cgacgccggc gacggtggcg agcacgacga tctcgatgcc
      661 ggacacgatc gggttccgcc tggtccacct cgacttcagg acaccgatgc cgaacatgac
      721 gacgccggag aagaccatcg atccccacac cgccgtgctg accggcagga tcatgtacgg
      781 gatcagcggc acgagcgcgg ccgtgccgaa cgagacgccc atgatgacgg cgccctgcag
      841 cgcgccgtgc ccttcttcga tgaccagccc gagctctttc tcgaccatcg tcttgagcag
      901 cacgctcggc tcgcgcgcga gctccgcggc gacacgtcgc gcggcggcct cacccagccc
      961 ctcgtgctcc agcatcagcg cgacctctgc ctcggcctcg tccggccggt cgcggacctc
     1021 ctcgcgctcg ttctcgatct gcgcgacgta gatctcacgc tggctcttcg acgacaggta
     1081 ttcgccggcg gccatcgaga agaccccggc gagggcagag gcgatacccg cgacgaggat
     1141 cgggtagcgg tctcccgtcg cgccgctgac cgcgctcgcg agcgcgagcg tgctcacgat
     1201 gccgtcctgc atgccgaaga gcgcctcgcg gatctcgccg agcatgtctg actcgcggcg
     1261 ctcctcggcc agatgctgct cgagccacgc gcgatcgaga tggcgtggct cggtcgtcgg
     1321 tcgcacgatg ccgtttagcg taccgtcgcc cgcgcgtcgc ggcgctcctc cggcggacgg
     1381 acaccgcgga aggagcgata gacgagcagg tcgtacgcga tcttgagcgc gccggcggcg
     1441 aagaacggca cgccgagcgc cgccaccgag aacgcgtacc cggtgagcgc gggggcgaag
     1501 gcggtcgcgg tgccccgcgc cacgttggtg agacccgcgg ccgcgacgcg ctcgtccgga
     1561 tcgacgacgg ccatcgtgta ggactgccgc gtcgggacgt ccatctgcga gatcgacatg
     1621 cgcgccagga acatggcggc cgcgagcgca cccgtcggca tcagcggcac gaggaccagc
     1681 agcacgttgg acgggatgtg cgtgaagacc atcgtgttga tgagcccgat gcgcatggcg
     1741 agcctgccgg ccacgagaag cgagagcccg gagaggatgt tcaccccgaa gaagaggacg
     1801 ccgagctcct gcggcgtgaa gccccagcgc aggtcgaacc agtacgcgac gatcgattgc
     1861 accaccaggc cgcccgcgaa ggcgtcgatc gcgaacagcg cggagagacg cgcgacgatc
     1921 ccgcgcgagc ggtggatgcc gagcagccgg cgctcgccct cgacacgggc gatctccacg
     1981 cggtccgaga gcgtccagaa gatcgccagg ttcgcgagcc cgacgatcgc gtagagcacg
     2041 aagagcgggc gatacgcatc ggctcccccc agcccggccg cggcggccag gccgacggcg
     2101 acgcccgcga agagcgcgcc gaccgcctgc gcggcgttcg cgacgaacgc gtagagcgag
     2161 aagagccagg tgcgctgctc ggccggcgcc gtctgcggca ggatcgcctg ctccacggtc
     2221 acgaacgcgc ccacctccga gctcgtcgcc gagatggtgc cggtgaatgc cgcgagcatc
     2281 aggaggtcga agctcgacgc gagcgcgaag agcgtcccgc cgagggccat gagcgccgag
     2341 agcaccatca ccgtccggcg ccggcccacg cggtcggcga tgagcgtcca gaccacggtc
     2401 atcgccgccg agccggcgac cgcggcggac aggacgaacc cgacctggag ctcgctgtac
     2461 cccaggcgcg cgaggtacac ggcgaggacg acggcgagat agccgtacgc gaaggtgcgc
     2521 aggatgcgcg tgagcaggag ccgccggccg tcggccgaca gccaggagag gtcgatgcct
     2581 agatcacgcg cccggcccga cccgcctcgt cgacgagcgg aaggcccgcg cgcgaccagg
     2641 cggtgacgcc gccgtcgaga ttctccgccg cgtagcccag ctgcttcagc ccgcggacgg
     2701 cggcggcact acgcgaaccg ctgcggcaga cggccacgat ggaccgtccg gccggcagct
     2761 ccgcgagccg gtcaccgagc tgaccgagcg ggatgtgctg cgcaccgtcg atgtggccgg
     2821 cgcgccattc gtcgtcctca cgcacgtcga ggaggtggtg ggcggcgcgg tccttgctga
     2881 gttcctccgg tcgcacccat tcattctgcg cgagaggagc cgtctttgga aaagagcaag
     2941 gcgcgcgagc gccttcgcac gctgcgcgag gaattcacgg cgaccgtgga cgcgatcagg
     3001 acgcgcctga cggactcgca gcgggagagc ggcggcgaca tctcgctcgc cgaccagcat
     3061 cccgccgacg tggccaccga gaccgccgac cgcgagctcg acacctcccg cggagccatg
     3121 ttcgaagcac gcatccgcca gatcgacgac gcgctcgagc ggctcgaagc cggcacgtac
     3181 gggacgtgtc tggtgtgcgg acagcccatc cccgacgagc ggctcgaggc gatccccgac
     3241 acgccgtact gcgtgaaaga cgccgagaag gagcaggctc gagcctcgta gcggccgctc
     3301 gcgcagaatg cgcccgtgca cccgccgtat ccggtcgcgc tcgtgccgct cataccggag
     3361 ttcctcacgc gacggcgtgt gaagttcacg tacccgctgc gcgtgatgg
//
LOCUS       DASTZR010000395         3027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_492418, whole genome shotgun sequence.
ACCESSION   DASTZR010000395 DASTZR010000000
VERSION     DASTZR010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492418"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VFV20_08815"
     CDS             <1..801
                     /locus_tag="VFV20_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5288490.1"
                     /translation="FKRVQFTPDLVPADLVGTRIYNQKSGEFQTSLGPVFTNLLLADE
                     INRAPAKVQSALLEVMQERQVTIGRETHRVPDPFLVMATQNPIETEGTYALPEAQVDR
                     FMMKVLVTYPTEMDEFVIVERMTGALAVVQPVMSTEQLVALQREVDRVFVDPVLMEYV
                     VRMVSATRMPKEYGLAESAKHITYGASPRASINLVLTARALAFMRGRDYVLAQDIHDV
                     AHDVLRHRIVLSYEGLSEDVTADALITKILERIPVPVAPMQGHVAARA"
     gene            893..1696
                     /locus_tag="VFV20_08820"
     CDS             893..1696
                     /locus_tag="VFV20_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEU5288491.1"
                     /translation="MDLADIREYVPGDDVRHIDWNVTARMDTPYVREYLEDREITAQF
                     LLDVSPSVDFGTATALKRDRLIDFVAVLSRLLTRQGNKVGAVIYGGHVEHVVPARSGR
                     VQVLRLIDELERRPRLERAPATALAELLETGLRSIRRRSLVFVVSDFFSTPGWERPLA
                     LLARRHEVLAIRLSDPRERELPDIGMVVMNDSETGEHVWVDTSDRGFRTRFAAAVLRR
                     DETLAGIFQRSGVESLELSTEDDMVRAVLRFAAKRKLRTARQRAMAVAS"
     gene            1693..2712
                     /locus_tag="VFV20_08825"
     CDS             1693..2712
                     /locus_tag="VFV20_08825"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU5288492.1"
                     /translation="MTFLWPGNLFLVLIVPALIAAYVWAQRRRQRYALRYASLSLVRE
                     ALGRGPGRKRHVPPAVFLAALFFMLAGTARPETVVTVPVQEGTVILALDVSGSMLAED
                     LKPNRMEAAKEAAKAFVARQGTDVKLGVVAFSGDAQIVQAPTSDKDLVVAAINRLRPQ
                     RATAIGRGMLASLDAIFEDSEEIAPSVIALRRLAGDATGPTPQPVPRGADRTATIVLL
                     SDGQNNQFPAPQQVVEDAVSRGIRIFTVGVGSADGTIVRIQGRAVRTRLDEATLKQIA
                     DATGGQYFNAANEKDLRAVYENLTTQLVFRQQRTEITAFLTAIAAVLSIVAASLSLAW
                     FNRIP"
     gene            complement(2780..>3027)
                     /locus_tag="VFV20_08830"
     CDS             complement(2780..>3027)
                     /locus_tag="VFV20_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288493.1"
                     /translation="HASTGATNRFDVAGSVVGGTATLAGTILNSTAWYLVGTPIVVVA
                     DTATGSISYTMGPITAGPFRGKTLTFVGDGTVLVNAG"
BASE COUNT          454 a         1077 c         1033 g          463 t
ORIGIN      
        1 ttcaagcgcg tccagttcac acccgacctc gttccggccg atctcgtcgg cacgcgcatc
       61 tacaaccaga agagcggcga gttccagacg tcgctcggcc ccgtgttcac caacctgctc
      121 ctcgccgacg agatcaaccg cgcgccggcg aaggtgcaga gcgctctcct cgaggtcatg
      181 caggagcgtc aggtgacgat cgggcgcgag acccaccggg tgcccgaccc gttcctcgtc
      241 atggccacgc agaaccccat cgagacggag ggcacctacg cgctcccgga ggcgcaggtg
      301 gaccgcttca tgatgaaggt gctcgtcaca tatcccacgg agatggacga gttcgtcatc
      361 gtcgagcgca tgaccggcgc cctcgcggtg gtccagccgg tgatgtcgac cgagcagctg
      421 gtggccctgc agcgcgaggt cgaccgggtc ttcgtcgatc ccgtgctcat ggagtacgtc
      481 gtacgaatgg tctcggccac gcgcatgccg aaggagtacg gcctcgcgga gtccgcgaaa
      541 cacatcacct atggcgcgag ccctcgtgcg tccatcaacc tcgtgctcac cgcgcgtgcg
      601 ctcgcgttca tgcgcggccg cgactacgtg ctcgcgcagg acatccacga cgtggcgcac
      661 gacgtgctcc gccaccgcat cgtgctctcg tacgaagggc tctcggagga cgtgaccgcg
      721 gacgcgctca tcacgaagat cctggaacgc atcccggtgc cggtggcgcc gatgcaggga
      781 cacgtcgctg cgcgcgcctg acgaccgcct gcggcggctt gaatggaccg tcatccgtcg
      841 tctcgacgga tggctccagg gcgactaccg cacgctgttc cgcggctacg gcatggacct
      901 cgcggacatc cgcgagtacg tccccggcga tgacgtccgc cacatcgatt ggaacgtcac
      961 ggcccgtatg gacacgccct acgtgcgcga gtacctcgag gaccgcgaga tcacggcgca
     1021 gttcctgctc gacgtgtcgc cgtccgtgga cttcggcacg gcgaccgcgc tgaagcgcga
     1081 ccggctcatc gacttcgtcg ccgtgctctc gcgcctcctc acgcggcagg gcaacaaggt
     1141 cggtgcggtc atctacggcg gccacgtcga gcacgtcgtc ccggcgcgca gcgggcgcgt
     1201 gcaggtgctg cggctcatcg acgagctcga gcggcggccg cgcctggagc gcgcaccggc
     1261 gacggcgctc gccgagctgc tcgagaccgg actgcggtcg atccgccggc gctcgctcgt
     1321 gttcgtcgtg tccgactttt tcagcacgcc gggctgggag cggccgctcg cgctgctcgc
     1381 gcggcggcac gaggtcctcg ccatccggct cagcgatccg cgggagcgag agctgcccga
     1441 catcggcatg gtcgtgatga acgactccga gaccggggag cacgtctggg tcgacacgtc
     1501 cgatcgtggt ttccgcacgc gcttcgccgc cgcggtgctg cgacgcgatg agacgctcgc
     1561 cggcatcttt cagcggagcg gcgtcgagtc gctggagctc tcgaccgagg acgacatggt
     1621 ccgggcggtc ctgcgcttcg ccgcgaagcg caagctgcgc acggcgcgcc agcgtgccat
     1681 ggcggtggcg tcgtgacctt cctctggccc gggaacctct tcctcgtgct catcgtgccc
     1741 gcgctcatcg ccgcgtacgt gtgggcgcag cggcggcgcc agcgctacgc gctgcgttac
     1801 gcgagcctct cgctggtgcg cgaggcgctc ggcaggggac ccggacgcaa gcgtcacgtg
     1861 ccgcccgcgg tcttcctcgc cgcgctcttc ttcatgctgg ccgggaccgc gcgtcccgag
     1921 accgtcgtga cggtgcccgt gcaggagggc accgtgatcc tcgcgctcga cgtgtccggc
     1981 agcatgctcg ccgaggacct gaagccgaac cgcatggagg ccgcgaagga ggccgcgaag
     2041 gcgttcgtcg cgcgccaggg cacggacgtg aagctcggcg tcgtcgcgtt ctccggcgac
     2101 gcgcagatcg tgcaggcgcc gacgagcgac aaggacctgg tggtcgccgc gatcaaccgc
     2161 ctccgtccgc agcgcgccac ggccatcggc cgcgggatgc tcgcgtcgct cgacgcgatc
     2221 ttcgaggact ccgaggagat cgcgccgtcg gtcatcgcgc tccgacggct cgccggcgac
     2281 gcgaccggcc cgaccccgca accggtgccg aggggcgccg accgcaccgc cacgatcgtg
     2341 ctgctgtcgg acgggcagaa caaccagttc ccggcgccgc agcaggtcgt cgaggacgcg
     2401 gtgagccgcg gcatccgcat cttcacggtc ggcgtgggca gcgcggacgg gacgatcgtg
     2461 cgcatccagg gccgcgcggt gcgcacgcgc ctcgacgagg cgacgctcaa gcagatcgcg
     2521 gatgccacgg gcggccagta cttcaacgcg gcgaacgaga aggacctgcg cgccgtgtac
     2581 gagaacctca cgacgcagct cgtcttccgt cagcagcgca cggagatcac ggcgttcctc
     2641 acggcgatcg cggcggtgct ctcgatcgtc gcggcgtcgc tctcgctggc ctggttcaac
     2701 cgcattccgt agctctcggc cgccgccgcg cggctccgat ctgcggagct gcgcgacggc
     2761 ttagccgcgg aggcgaccgc taccccgcgt tgacgaggac ggtgccatct ccgacgaacg
     2821 tcagcgtctt gcccctgaac ggaccggccg tgatcggccc catcgtgtag ctgatgctgc
     2881 cggtggccgt atccgcgacg acgacgatgg gcgtaccgac gaggtaccag gccgtggaat
     2941 tcaagatcgt gcctgccagg gtcgcagtgc caccgacgac gctacccgcg acatcgaaac
     3001 gattcgtggc gcccgtcgat gcatgtg
//
LOCUS       DASTZR010000396         2018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_544433, whole genome shotgun sequence.
ACCESSION   DASTZR010000396 DASTZR010000000
VERSION     DASTZR010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544433"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VFV20_08835"
     CDS             <1..426
                     /locus_tag="VFV20_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5288494.1"
                     /translation="LPIWIGGGGEKRTLRTVARYADGWNLPYLSPAEWARKNAVLDRW
                     CEREHRDPRAVARTANVGIYTGADDAGRRRASERFVAEWGPNETRGGFLRGSASEAAA
                     LVAGYRDAGVEQLNLVVRAGPYDWDALTQFAEVVAPALR"
     gene            423..956
                     /locus_tag="VFV20_08840"
     CDS             423..956
                     /locus_tag="VFV20_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012076110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5288495.1"
                     /translation="MNDQGGDITLLDADGLAAAVPELATILADAVASGASVGFVTPFT
                     AEDAALWWRSLTSDVAAGRTLLLLLRERGRAVGTAQLRLAPLPNARHRAEVAKVLVLR
                     EARHRGHATALMRRIEDLARTHGRTLLVLDTITGSEAARLYDELGWTRSGDIPRYAAM
                     PDGRLEPTTVFFKELAP"
     gene            953..1333
                     /locus_tag="VFV20_08845"
     CDS             953..1333
                     /locus_tag="VFV20_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288496.1"
                     /translation="MSRPALRLEPREMAPVNGAWRVRWRVTNGGDQHVRLVTMRAPHG
                     RFHSDPLDLNVVVVQTATVEHTVRIEAAPGEDVENAFVIFVAVQGKDTWRVLFRVRIH
                     LGDDGTPAPLVESVSAHRVGFSEV"
     gene            complement(1550..>2018)
                     /locus_tag="VFV20_08850"
     CDS             complement(1550..>2018)
                     /locus_tag="VFV20_08850"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEU5288497.1"
                     /translation="HAGISREEADAADPAPGTLAAIEMIRSGCQNRSALEGIALLAFV
                     EAMHGGPDGAAARVYRELTGYYGFPPEAAATYELHAEQDTGHGDRQIAVIHQYARDET
                     SQEKVRRAVRLGCEAFNFEWDGHVQAMTGRRDVYWSGKAPLALRHPEVRLPRG"
BASE COUNT          286 a          717 c          726 g          289 t
ORIGIN      
        1 ctgccgatct ggatcggcgg cggcggcgag aagcgcacgc tccgcaccgt ggcacggtac
       61 gcggacggct ggaacctgcc gtacctctcg ccggcggagt gggcgcgcaa gaacgccgtg
      121 ctcgaccgct ggtgcgagcg cgagcaccgc gatccgcgtg cggttgcgcg caccgccaac
      181 gtcggcatct acacgggcgc cgatgacgcg ggccggcgac gcgcatcgga gcgtttcgtc
      241 gccgagtggg ggccgaacga gacgcgcggc ggattcctgc gcgggagcgc cagcgaagcc
      301 gcggcgctcg tcgcggggta ccgcgacgcg ggcgtcgagc agctcaacct cgtcgtgcgc
      361 gcgggcccgt atgactggga cgcgctcacg cagttcgccg aggtcgtcgc gccggcgctc
      421 cgatgaatga tcagggtggc gacatcacgc tgctggacgc cgacggtctc gccgccgccg
      481 tccccgagct cgccacgatc ctcgcggacg ccgtcgcgtc gggcgcgtcc gtcgggttcg
      541 tcacgccgtt cacggccgag gacgcggcgc tctggtggcg ctctctcact tcggacgtcg
      601 ccgccggccg cacgctgctc ctgctcctgc gcgagcgcgg ccgcgcggtc ggcaccgcac
      661 agctgcgcct cgcgccgctc ccgaacgcac gccaccgcgc ggaggtggca aaggtcctcg
      721 tcttacgtga agcacggcac aggggccacg ccaccgcgct catgcggcgg atcgaggacc
      781 tcgcgcggac tcacgggcgg acgctgctcg tgctggacac catcaccggc agcgaggcag
      841 cgcgcctgta cgacgagctc ggctggacgc ggtccggcga catcccgcgc tacgcggcga
      901 tgccggacgg caggctcgag cccacgacgg tcttcttcaa ggagctcgcg ccgtgagccg
      961 gccggccctg cgcctcgagc cgcgtgagat ggccccggtg aacggcgcgt ggcgcgtgcg
     1021 ctggcgcgtc accaacggag gcgatcagca cgtccgcctc gtgaccatgc gcgcgccgca
     1081 cgggcgcttt cactcggatc cgctggatct caacgtcgtc gtcgtgcaga ccgcgacggt
     1141 cgagcacacc gtgcgcatcg aggctgcgcc gggcgaggac gtcgagaacg ccttcgtcat
     1201 cttcgtcgcc gtccagggaa aggacacctg gcgcgtgctc tttcgcgtgc ggatccacct
     1261 gggcgacgac ggcacgccgg cgccgctggt cgagtcggtg agcgcgcacc gcgtcgggtt
     1321 ctcggaggtg tagatgaacg cgcacgatcg caacgtcatg aaccggatga agggctgacg
     1381 cggcaactga agcctcggct tcgaagagac cacgggtatg gtctccaacg tgctcgagat
     1441 ggagcaggac gagctggtcg cattgctcag gaccttcaag aagaagtacg cggacgatcc
     1501 cgagtaccgg gacgcgcgcg ccgcgctccc gaagacctgg ccgttctgat cagccgcgcg
     1561 ggagccgcac ctcggggtga cgtagcgcga gcggagcctt tccgctccag tacacgtcgc
     1621 gccgtccggt catggcctgc acgtggccgt cccattcgaa gttgaatgcc tcgcacccga
     1681 ggcgcaccgc gcggcgcacc ttctcctgcg acgtctcgtc gcgcgcgtac tggtggatga
     1741 ccgcgatctg gcggtcgccg tgaccggtgt cctgctccgc gtggagctcg tacgtggcag
     1801 cggcctcggg cggaaagccg taatacccgg tgagctcgcg gtacacgcgc gcggcggcac
     1861 cgtccggtcc gccgtgcatc gcctcgacga aggcgaggag cgcgatgcct tcgagcgcgc
     1921 ttcggttctg gcaaccggag cggatcatct cgatcgccgc gagcgtcccg ggcgccgggt
     1981 ccgcggcgtc ggcctcctcg cgcgagatcc cggcgtgc
//
LOCUS       DASTZR010000397         2178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_356601, whole genome shotgun sequence.
ACCESSION   DASTZR010000397 DASTZR010000000
VERSION     DASTZR010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356601"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VFV20_08855"
     CDS             complement(<1..440)
                     /locus_tag="VFV20_08855"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5288498.1"
                     /translation="MKLGVIVSPAAGWSYDELLELATASETAGFDSFWVSDHLFGGEG
                     FPDRDCLEAWTLLAALSRDTRTIRLGCLVTAAQYRNPALLAKIVASVDQLSGGRVDFG
                     IGAGWKVNEYAAYGYDFAEPGARVTQMIEAIEICRRLWTEERAT"
     gene            490..1206
                     /locus_tag="VFV20_08860"
     CDS             490..1206
                     /locus_tag="VFV20_08860"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5288499.1"
                     /translation="MVGENESIDIPVASPVGALLLHGLFGTPNELHVLGGQIARAGYR
                     VRIPLLPGRGANLDALDRLSWREVRDAALAEYEELAREHDQVVVGGLSAGATLALDLA
                     LLRPPAGLLLYAPALSIRYRAVNLAPYVWRVLRRWPMPGDGRRVPLRAIGELVGAMRR
                     VRAGLGRIHAPALVIHSLDDPLTPASSGILVSRELGGPVKLVLREGATHAVTAGAERD
                     EIGALTLTWLRELLPLAAIP"
     gene            complement(1163..>2178)
                     /locus_tag="VFV20_08865"
     CDS             complement(1163..>2178)
                     /locus_tag="VFV20_08865"
                     /inference="COORDINATES: protein motif:HMM:NF005560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288500.1"
                     /translation="RTDWEAMDDVPALDWLRQACGPRAVESVWTPLMLGKFGEDAARI
                     PLAWLWSKFVLRRKLRGGGANREELGYPRGSFREICVALADDIRARGGEIHVDREVLR
                     VRADGDYVLDCAGPGAYRTQPGTEARAYEHRADVVLFTTPAFVTRRLTEWPAAYAARL
                     DDWTYETAIVLLLELRHQYSDTYWINVADTSVPFLGLVEHTNLVPAERYPARYLYVSN
                     YVHRDDPLSRLDGEALLRHYLHALRLMNPRFDERHVLRHWSFREEAAQPVPRVGNRHR
                     LLPYASPRRGLYLANTTQIYPEDRGTNYSVRLGRDVAEEIARDVPAALTGSPRAAGAP
                     AAM"
BASE COUNT          298 a          770 c          791 g          319 t
ORIGIN      
        1 taggtcgcgc gctcctcggt ccagagcctg cggcagatct cgatggcctc gatcatctgc
       61 gtgacgcgcg cgcccggctc cgcgaagtcg tagccgtagg cggcgtactc gttcaccttc
      121 cagccggcgc cgatgccgaa gtcgacgcgc ccgcccgaga gctgatcgac gctcgcgacg
      181 atcttcgcga ggagggccgg attccggtac tgcgccgcgg tgacgagaca gccgaggcgg
      241 atcgtccgcg tgtcgcgaga cagcgccgcg aggagcgtcc atgcctcgag gcagtcgcgg
      301 tcggggaagc cctcgccgcc gaagaggtga tcgctgaccc agaacgagtc gaatcccgcg
      361 gtctcgctcg cggtagccag ctccagcagc tcgtcgtacg accatccggc ggcgggggag
      421 acgatgacgc cgagcttcac cgcgggcatg gtaggtgccg cgcacgccac gtgccgtacc
      481 ttcgtgcgta tggtcggtga gaacgagagc atcgacatcc ccgtcgcgtc accggtcggg
      541 gcgctcctgc tgcacggcct cttcgggacg ccgaacgagc tgcacgtcct cggcgggcag
      601 atcgcgcgcg cgggctaccg cgtgcgcatc ccgctgctgc ccgggcgtgg cgccaacctc
      661 gatgcgctcg accggctctc gtggcgcgag gtgcgcgacg ccgcgctcgc cgagtacgag
      721 gagctcgcac gggagcacga ccaggtcgtc gtcggtggtc tctccgcggg cgccacgctg
      781 gccctggacc tcgccctgct ccgcccgccc gcgggcctgc tgctgtacgc gcccgcgctc
      841 tcgatccgct accgcgcggt caacctcgcg ccgtacgtgt ggcgcgtgct gcgccgatgg
      901 ccgatgccgg gcgacggacg gcgcgtcccg ctgcgcgcga tcggcgagct ggtgggcgcg
      961 atgcgccgcg tgcgcgccgg actcgggcgc atccacgcgc ccgcgctggt gatccattct
     1021 ctggacgatc cgctcacgcc cgcgtcgagc gggatcctcg tgtcgcgcga gctgggcgga
     1081 cccgtgaagc tcgtgctgcg ggaaggtgcg acgcacgcgg tgaccgcggg tgcggagcgc
     1141 gacgagatcg gcgcgctcac gctcacatgg ctgcgggagc tcctgccgct cgcggcgatc
     1201 ccgtgagcgc cgccggcacg tcgcgggcga tctcttcggc gacgtcgcgg ccgagccgga
     1261 cgctgtagtt ggtgccacgg tcctcggggt agatctgggt cgtgttcgcg aggtacaggc
     1321 cgcgccgcgg cgacgcgtag gggaggaggc ggtgtcgatt gccgacgcgc ggcaccggct
     1381 gcgcggcctc ctcgcggaag gaccagtgac gaaggacatg gcgctcgtcg aagcgcggat
     1441 tcatcagcct cagtgcgtga aggtagtggc ggaggagcgc ctcgccatcc aggcgcgaca
     1501 gcgggtcgtc gcgatgcacg tagttcgaca cgtacaggta gcgcgccgga taacgctcgg
     1561 ccggcacgag attggtgtgc tcgacgagcc cgaggaaagg gacgctcgtg tccgcgacgt
     1621 tgatccagta cgtgtcgctg tactggtgcc gtagctcgag gagcaggacg atcgcggtct
     1681 cgtaggtcca gtcgtcgagt cgcgcggcgt acgcggcggg ccactcggtc agccggcgcg
     1741 tcacgaacgc aggcgtcgtg aacagcacga cgtccgcgcg atgctcgtat gccctcgctt
     1801 cggtgcccgg ctgcgtccgg tacgcgcccg ggcccgcgca gtccaggacg tagtcgccgt
     1861 ccgcgcggac ccgcaggacc tcgcgatcga cgtgtatctc cccgccacgc gcgcggatgt
     1921 cgtccgcgag cgcgacgcag atctcgcgga acgatccgcg cgggtatccc agctcctcac
     1981 gattcgcgcc gccgccccgg agcttccggc gcaggacgaa cttcgaccac agccacgcga
     2041 gcgggatgcg cgcggcgtcc tcgccgaact tcccgagcat gagcggcgtc cacaccgact
     2101 cgacggcgcg cgggccacac gcctggcgga gccagtcgag cgccggtacg tcgtccatcg
     2161 cctcccagtc cgtgcgcg
//
LOCUS       DASTZR010000398         2147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_936328, whole genome shotgun sequence.
ACCESSION   DASTZR010000398 DASTZR010000000
VERSION     DASTZR010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936328"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VFV20_08870"
     CDS             complement(<1..435)
                     /locus_tag="VFV20_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HEU5288501.1"
                     /translation="MLTDYHVHLRPDEELQGSVERFFTRSNAERYREHASERGIEELG
                     VAEHVYRFHQALEVWEHPFWREYAVDDLDDYCSFVRDETDLKLGIEADFIPGREDRIA
                     NLLDAREWDYVVGSVHFVGDQSLDTEEFTVWTQTASADRIWKR"
     gene            complement(429..1076)
                     /gene="ispD"
                     /locus_tag="VFV20_08875"
     CDS             complement(429..1076)
                     /gene="ispD"
                     /locus_tag="VFV20_08875"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEU5288502.1"
                     /translation="MLVLGILAGAGAGDRLGADAPKAFIRCAGRELWEWSADVLAREC
                     DTLVFAVPADRVDADAPARDDGIRRVAGGASRSESVRNALAVAPDADIVVIHDAARPF
                     VTAELVRECIRAVEAGADGAIAAAPVTDTIKEADADRRVVRTLDRSSLWSIQTPQAFR
                     ADVLRRALDGDVSSATDDASLVEAAGGRVQIVEAPPGNFKITWPEDLARAESLLC"
     gene            complement(1079..1639)
                     /locus_tag="VFV20_08880"
     CDS             complement(1079..1639)
                     /locus_tag="VFV20_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEU5288503.1"
                     /translation="MESELGEIEFAPAPPANYKVGDHVVYPHHGAGKVEKKEMKELLG
                     EKREYLTIRIIHNDMTVMVPCEKAGQVGLRNIIGASAVKKVMGVLTADVSTMPKNWNR
                     RFKYNREKIKTGDIYELAEVVRNLAIREFEKGLSTGEKQMYTRARKILASELMYALDM
                     DEEEAEEHLTEVMREAHAGRGGATKK"
     gene            complement(1679..>2147)
                     /locus_tag="VFV20_08885"
     CDS             complement(1679..>2147)
                     /locus_tag="VFV20_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933164.1"
                     /note="non-specific DNA-binding; scans chromosomes during
                     sporulation for DNA-damage; delays initiation of
                     sporulation; participates in a checkpoint signaling
                     cascade for cell-cycle progression and DNA repair; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HEU5288504.1"
                     /translation="RYIVELGTEGRLIEMQLEEVMVGVAAERGALVHDYMVDDSEDGF
                     ALVLDSLARLPHQDLLDFGRLAELLGYDRKVNTLDHPVAPRGRRILGRIPRLPKLLIQ
                     RIVSEFGGLEEILAANDAELEAVEGVGDVRAKEIREGLRRLQEINLVDRYLQT"
BASE COUNT          337 a          734 c          686 g          390 t
ORIGIN      
        1 gcgcttccag atccggtcgg cggaagcggt ctgcgtccag accgtgaact cctcggtgtc
       61 cagcgactgg tcgcctacga agtggaccga gccgacgacg tagtcccact cgcgcgcgtc
      121 gagcaggttg gcgatccggt cctcacggcc ggggatgaag tcggcctcga tgcccagctt
      181 caggtccgtc tcgtcacgga cgaacgagca gtagtcgtcg agatcgtcga cggcgtactc
      241 acgccagaac gggtgctccc agacctcgag tgcctgatgg aagcggtaga cgtgctcggc
      301 cacacccagc tcctcgatcc cgcgctcgga cgcatgctcg cggtagcgct cggcgttgga
      361 gcgcgtgaag aaccgctcga ccgagccctg cagttcctcg tccggtcgca ggtggacgtg
      421 gtagtccgtc agcacagcag cgattctgcc cgggcgaggt cctccggcca tgtgatcttg
      481 aagttgcctg gaggcgcttc aacgatctgc acccggccac cggccgcctc aacgagcgaa
      541 gcgtcgtcgg ttgcggatga gacgtcgcca tccaaagcgc ggcggaggac atccgcgcgg
      601 aacgcctgcg gcgtctggat cgaccacagc gacgaccggt caagcgtccg caccacgcga
      661 cgatccgcat cggcttcctt gatcgtgtcc gtcacgggcg cggccgcgat tgcgccatcc
      721 gcccccgctt ccacggcgcg gatgcattcg cgcacgagct ccgccgttac gaacggccgc
      781 gcggcgtcgt gaatcacgac gatgtcggcg tcgggcgcga ccgcgagcgc attgcgaacg
      841 gactccgacc gcgacgcgcc gccggcgacg cggcggatgc cgtcgtcacg cgcgggcgca
      901 tccgcgtcga cgcggtcggc gggcacggcg aacacgaggg tgtcgcactc acgggcgagc
      961 acgtccgcgc tccactccca cagctcccgg ccggcgcagc ggatgaacgc cttgggggca
     1021 tcagccccca ggcgatcgcc tgcgccggct ccggcgagga ttcctagtac cagcagcgct
     1081 acttcttggt cgcgccgccg cggccggcgt gcgcctcgcg catgacttcc gtgaggtgct
     1141 cctccgcctc ttcctcatcc atgtcgagcg cgtacatcag ctccgacgcg aggatcttgc
     1201 gggcacgcgt gtacatctgc ttctcgccgg tcgacaagcc cttctcgaac tcgcgaatcg
     1261 caaggttgcg gacgacctcg gccagctcgt agatgtcgcc ggtcttgatc ttctcgcggt
     1321 tgtacttgaa gcggcggttc cagttcttcg gcatggtgct gacgtcggcc gtcaacacgc
     1381 ccatgacctt cttgacggca ctcgccccga tgatgttgcg gaggcctacc tgtccggcct
     1441 tctcacacgg gaccatgacg gtcatgtcgt tgtggatgat gcggatggtg aggtattccc
     1501 gcttctcacc gaggagttcc ttcatctcct tcttttccac cttgccggca ccgtggtgcg
     1561 ggtacaccac gtggtcgccc accttgtagt tggcaggagg tgccggggcg aattcgatct
     1621 ccccgagctc tgactccaac actgaatcgc tgatgacgtc ctcctagcct tccttacttc
     1681 acgtttgcag gtagcggtcc acgagattga tttcctgcag gcggcgaagg ccctcgcgga
     1741 tctccttcgc gcgtacgtcg ccgacgccct cgactgcctc cagctccgca tcattcgcgg
     1801 ccaggatctc ttcgagtcct ccgaactcgg agacgatgcg ctggatcagc agcttcggaa
     1861 ggcgcgggat gcgcccgaga atccggcgcc cgcgtggggc aaccgggtgg tcgagcgtgt
     1921 tgaccttgcg gtcgtagccc agtagctcgg ccagacggcc gaagtccaac aggtcctgat
     1981 ggggaagtct tgcgagcgag tcgaggacga gtgcgaaccc atcctcggag tcgtcgacca
     2041 tgtagtcgtg cacgagcgcg ccgcgctcgg cggcaacgcc gaccatgacc tcctcgagct
     2101 gcatctcgat gaggcgaccc tcggtgccga gctcgacgat gtagcgc
//
LOCUS       DASTZR010000399         2829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_826580, whole genome shotgun sequence.
ACCESSION   DASTZR010000399 DASTZR010000000
VERSION     DASTZR010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826580"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..1085
                     /locus_tag="VFV20_08890"
     CDS             72..1085
                     /locus_tag="VFV20_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014126046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HEU5288505.1"
                     /translation="MTVTTGQLGLARFFEKRGSIPEIVIFAGKGGLGKTTSSAALSHY
                     LSQDLGKRTLCFSTDPQASLSDIFEQNFFGLGVTELKPRLNVVEIDADRRVAEYQKEV
                     KQKIKDMHGLDEIPPEIDEYIDSTSAEPAMYESATYDAMAELVAKREFEHYVFDMPPF
                     GHGVRMVAMADILSKWVEKITEARGKVQEYDAVAATLKGQKVSEDAVMGELIAIRTKI
                     KTFTDLIVDRKRTAFFMVLIPEKMAILDTDRALGMFHALGIDMSGIVVNQVYPEELLQ
                     KPNLSNLLRNRIEMQQRYLKEIREKFGDYVVSAIPMYDREPKGMDMIARTSVDLLKSP
                     VRI"
     gene            1085..2116
                     /locus_tag="VFV20_08895"
     CDS             1085..2116
                     /locus_tag="VFV20_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HEU5288506.1"
                     /translation="MDKLAVFLESHPDLKFVFTGGKGGVGKTVTAAALALYFAGKGKK
                     VMLASLNPVHSLSSVFDQDLSGGKSRQVQDGNLGGRLVAIEVETKDVVERYRENIGKR
                     VREFLKWSDIPVDAKPFVDIAVTNPAFEESAMFDKMTDVMLTEGHDYDVIVFDTAAVA
                     NAIRLIGLSKIYGLWLGRMIESRKDALSLRVQLSFRKEKIMDEVKKDPLMADLIDMND
                     RFNKVKDVLLDPKRTAFFFVTLPLALPISVVERFIESVKSYGVPVGGVVVNQVLPREV
                     VEQHRDETYLANKFDEQQGYLRRIKTNLGPLVRAQLPLYEEEVRGMAAMQAIARDLFS
                     YEPDFWATL"
     gene            2137..>2829
                     /locus_tag="VFV20_08900"
     CDS             2137..>2829
                     /locus_tag="VFV20_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017982724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU5288507.1"
                     /translation="MHGLRVVELSAFVAAPLGGATLAELGAEVVRIDPPGGGIDAHRW
                     PLHQGRSLYWAGLNQGKRSVNIDTRTAKGQALVTDLVARAGVLLTNLPVREWSTYERL
                     SARRPDLIMAVITGNPDGTTAVDYTVNAAVGIPFLTGPEGHDGPVNHVLPAWDALTGY
                     AVATAIVAAELHRVRTGEGQLITLSLMDVALSTVGHLGLIGEAQLVAEPRGRYGNDVY
                     GTFAHDFRTSDGR"
BASE COUNT          517 a          976 c          906 g          430 t
ORIGIN      
        1 acttcaccga cgcgatctac taggccggtc ggtcgcctcg agacgacaaa gcaggtgagg
       61 ggagtagcgc gatgaccgtc acgacgggcc agctcggcct cgcgcgcttc ttcgagaagc
      121 gcggcagcat ccccgagatc gtcatcttcg cgggcaaggg cggcctcgga aagacgacga
      181 gctccgcggc gctctcgcac tacctgtccc aggacctcgg gaagcgcacc ctctgcttct
      241 ccaccgaccc gcaggcctcg ctctccgaca tcttcgagca gaacttcttc gggctcggcg
      301 tcaccgagct gaagccgcgc ctcaacgtcg tcgagatcga cgcggaccgc cgcgtggccg
      361 agtaccagaa agaggtcaag cagaagatca aggacatgca cggcctcgac gagatcccgc
      421 cggagatcga cgagtacatc gattcgacct ccgccgagcc cgcgatgtac gagtccgcga
      481 cgtacgacgc gatggccgag ctcgtcgcga agcgcgagtt cgagcactac gtcttcgaca
      541 tgccgccctt cggccacggc gtccgcatgg tcgccatggc cgacatcctc tcgaagtggg
      601 tcgagaagat caccgaggcg cgcggcaagg tgcaggagta cgacgccgtc gccgccacgc
      661 tcaagggcca gaaggtgagc gaggacgccg tcatgggcga gctcatcgcg attcggacga
      721 agatcaagac gttcaccgac ctcatcgtcg accgcaaacg caccgcgttc ttcatggtgc
      781 tcatccccga gaagatggcg atcctcgaca ccgaccgcgc gctcggtatg ttccacgcgc
      841 tcggcatcga catgagcggc atcgtggtga accaggtcta ccccgaggag ctgctccaga
      901 agccaaacct ctcgaacctc ctgcgcaacc gcatcgagat gcagcagcgg tacctcaagg
      961 agatccggga gaagttcggc gactacgtgg tctcggcgat cccgatgtac gaccgcgagc
     1021 ccaaggggat ggacatgatc gcgcgaacgt ccgtcgacct gctcaagtcg ccggtgcgca
     1081 tctgatggac aagctcgccg tcttcctcga gagccacccc gacctgaagt tcgtcttcac
     1141 cggcggcaag ggcggcgtgg gaaagaccgt gaccgcggcc gcgctcgcgc tctacttcgc
     1201 gggcaagggc aagaaggtga tgctcgcgtc gctcaacccg gtccactcgc tgtcgagcgt
     1261 cttcgaccag gacctctccg gcggcaagtc gaggcaggtg caggacggca acctcggcgg
     1321 gcgcctcgtc gcgatcgagg tcgagacgaa ggacgtcgtg gagcgctacc gcgagaacat
     1381 cggcaagcgc gtgcgcgagt tcctcaagtg gtcggacatc ccggtcgacg cgaagccgtt
     1441 cgtcgacatc gccgtcacga acccggcctt cgaggagtcg gcgatgttcg acaagatgac
     1501 cgacgtcatg ctcaccgagg ggcatgacta cgacgtcatc gtctttgaca ccgccgcggt
     1561 cgcgaacgcg atccggctca tcggactctc gaagatctac ggtctctggc tcggccgcat
     1621 gatcgagagc cgcaaggacg cgctctcact gcgcgtccaa ctctcgttcc gcaaggagaa
     1681 gatcatggac gaggtcaaga aggacccgct catggccgac ctcatcgaca tgaacgaccg
     1741 cttcaacaag gtgaaggacg tcctcctcga ccccaagcgc accgcgttct tcttcgtcac
     1801 gctccccctc gcgctgccca tctccgtggt cgagcgcttc atcgagagcg tgaagagcta
     1861 cggcgtcccg gtcggcggcg tggtcgtgaa ccaggtgctg ccgcgcgagg tcgtggagca
     1921 gcaccgcgac gagacgtacc tcgcgaacaa gttcgacgag cagcagggct acctgcgccg
     1981 catcaagacg aacctcggtc cgctcgtgcg cgcgcagctg ccgctgtacg aggaggaagt
     2041 gcgcggcatg gccgcgatgc aggccatcgc gcgcgacctg ttctcgtacg agccggactt
     2101 ctgggcgacg ctctagacac gagcgcgaag ccggtcctgc acggcctccg cgtggtcgag
     2161 ctctccgcgt tcgtcgccgc gccgctcggt ggcgccacgc tcgccgagct cggtgccgag
     2221 gtcgtgcgca tcgacccgcc gggcggcggc atcgacgcgc accgctggcc gctccatcag
     2281 ggccgcagcc tgtactgggc cgggctcaat cagggaaagc gctcggtcaa catcgacaca
     2341 cgtactgcga aggggcaggc gctcgtcacc gatctcgtcg cgcgcgccgg cgtgctgctc
     2401 acgaacctgc ccgtccgcga atggtctacg tacgagcgcc tctccgcgcg gcggcccgac
     2461 ctcatcatgg cggtcatcac cggcaatccc gacggcacga ccgcggtcga ttacacggtg
     2521 aacgccgcgg tcgggatccc attcctgacc ggaccggagg gccacgacgg gcccgtgaac
     2581 cacgtgctcc ccgcgtggga cgcgctcacc ggctacgcgg tggcgaccgc gatcgtcgcg
     2641 gccgagctgc accgcgtgcg caccggggag ggacaactca tcacgctcag cctgatggac
     2701 gtcgcgctct cgacggtcgg gcatctcggg ctcatcggcg aggcgcagct cgtcgcggag
     2761 ccgcgcgggc gctacggcaa cgacgtgtac ggcacgttcg cgcatgactt ccgcacatcc
     2821 gacggccgc
//
LOCUS       DASTZR010000400         2677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_309478, whole genome shotgun sequence.
ACCESSION   DASTZR010000400 DASTZR010000000
VERSION     DASTZR010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309478"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1108)
                     /locus_tag="VFV20_08905"
     CDS             complement(68..1108)
                     /locus_tag="VFV20_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5288508.1"
                     /translation="MTPRVGMLAPISWRVPPRHYGPWERVVSLLTEGLVERGVDVTLF
                     ATADSVTAARLESVCPRPLSEDASLDAKVWESLHIANAFERSRALGLDVLHNHYDFLP
                     LTYTGGFDIPVVMTIHGFSSERIVPVYERYDGRVHYVSVSDADRHPRLTYARTVYHGI
                     ALEEFTPRDRPGTYLVFLGRMHSDKGVAEAIEVARRADLPLVLAGIIHDEEYFAREIA
                     PHVDDVHVRYVGSAGPKERDELLGNALALIHLVNFAEPFGLSMVEAMACGTPVIARRR
                     GSIPEVVEDGLTGFVVDDVAGAVAAVGRVSGLDRRAIRARCAERFSRDRMVNDYLSVY
                     DEVLAQQGARTR"
     gene            complement(1105..1590)
                     /locus_tag="VFV20_08910"
     CDS             complement(1105..1590)
                     /locus_tag="VFV20_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU5288509.1"
                     /translation="MPRIQKDIVVDVPVTTAYDQWTQFESFPQFMEGVKEVVQLDEKT
                     LRWRAKVGTKEEEWEAEITEQVPDRHVAWRSTTGAPNAGSVVFDSEGADRTRVSLEIS
                     YEPRDTGEKVGDALGVLDRRVDGDLKRFKEFIERRQAPTGAWRGEIHGGQVERGSGSK
                     T"
     gene            complement(1624..1902)
                     /locus_tag="VFV20_08915"
     CDS             complement(1624..1902)
                     /locus_tag="VFV20_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288510.1"
                     /translation="MDWIGAILESIREMECPCCGARLQSCAVRGITAESDALVVRLAC
                     VVCGENSVAVVRRDAANAPAPITKDDVLDAHEFLKRWHGPVAELISAA"
     gene            complement(1964..>2677)
                     /locus_tag="VFV20_08920"
     CDS             complement(1964..>2677)
                     /locus_tag="VFV20_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015182467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5288511.1"
                     /translation="GDSGIGRAVAAAFAREGADVAIVYLEEHEDAAETKRLVEAEGRR
                     CESIAGDVGDEAFCKRAVARTVRALGRLDVLVNNAAEQHPKDDISEITARQLERTFRT
                     NIFAMFYLVKAALPHLGEGSAIVNTTSVTAYRGSPTLLDYSSTKGAIVAFTRSLSINL
                     ADRKIRVNAVAPGPIWTPLIPSTFPPDKVESFGADTPMGRPGQPEEVAPAFVFLASDD
                     GSYVSGQVIHPNGGEVVNA"
BASE COUNT          400 a          959 c          899 g          419 t
ORIGIN      
        1 aactcgcgcc acatgcgcat cgccgtccgc gacggccagt agttcacgcc gacgcggaag
       61 gcgtccatca gcgcgtccgc gcgccctgct gcgcgaggac ctcgtcgtaa acggacaggt
      121 agtcgttcac catccggtcg cggctgaagc gctcggcgca gcgcgcgcgg atggcccgcc
      181 ggtcgaggcc ggacacacgc ccgaccgcgg ccacggcgcc cgcgacgtcg tcgacgacga
      241 agccggtgag cccatcctcc acgacctcgg ggatcgaccc gcgacgccgg gcgatcaccg
      301 gggtcccgca tgccatcgcc tcgaccatcg acagtccgaa cggctcggcg aagttcacga
      361 ggtggatgag cgcgagggcg ttcccgagga gctcatcgcg ctccttcggg ccggcggacc
      421 cgacgtagcg gacgtggacg tcgtccacgt gcggcgcgat ctcccgcgcg aagtactcct
      481 cgtcgtggat gatccccgcg aggaccagcg gcaggtcggc gcgccgcgcg acttcgatcg
      541 cctccgccac gcccttgtcg gagtgcattc gtcccaggaa gaccagatac gtgcccgggc
      601 ggtcgcgcgg cgtgaactcc tccagcgcga tgccgtgata gacggtgcgc gcgtaggtga
      661 ggcgcgggtg ccggtcggcg tcgctgaccg acacgtaatg gacgcgcccg tcgtaccgct
      721 cgtagacagg gacgatccgt tccgacgaga aaccgtggat cgtcatcacc acgggaatgt
      781 cgaagccgcc ggtgtacgtc agcggcagga agtcgtagtg gttgtggagc acgtcgagcc
      841 ccagcgcgcg cgagcgctcg aacgcgttcg cgatgtgcag gctctcccac accttcgcgt
      901 cgagcgacgc gtcctcggaa agcgggcgcg gacagacgct ctccagccgc gcggcggtga
      961 ctgaatccgc ggtcgcgaag agcgtcacgt ccacgccgcg ctcgacgagc ccctccgtga
     1021 ggagtgagac gacgcgctcc cagggcccgt agtggcgggg cggcactcgc cacgagatcg
     1081 gggcgagcat gccgacccgc ggcgtcacgt cttcgagccg ctgccccgct cgacctgccc
     1141 gccgtggatc tcgccacgcc acgcgccggt cggtgcctgg cggcgctcga tgaactcctt
     1201 gaagcgcttg agatccccat cgacgcgccg gtcgaggacg ccgagcgcgt cgccgacctt
     1261 ttcgccggta tcgcgtggct cgtacgagat ctcgagcgat acgcgcgtcc gatcggcgcc
     1321 ctcggagtcg aacacgacgg agcccgcgtt cggcgcgccg gtggtgctgc gccacgcgac
     1381 gtgacggtca ggcacctgct ccgtgatctc cgcctcccat tcctcttcct tggtgccgac
     1441 cttcgcgcgc cagcggagcg tcttctcgtc gagctgcacg acttccttca cgccctccat
     1501 gaactgcggg aagctctcga actgcgtcca ctggtcgtac gcggtcgtca ccggaacgtc
     1561 gacaacgatg tccttctgga tacgcggcat gacttcctcc tccgtacttg gcgcgtcgcg
     1621 ggactacgcg gcgctgatga gctcggcgac cggaccgtgc cagcgcttca ggaattcgtg
     1681 cgcgtcgagc acgtcgtctt tcgtgatggg agcgggcgcg ttcgccgcat cgcgccgcac
     1741 caccgcgacg ctgttctcgc cacagacgac gcacgcgagc cgcacgacca gcgcgtcgct
     1801 ctcggcggtg atgccgcgca cggcgcagct ctggaggcgc gcgccgcagc acgggcactc
     1861 catctcgcgg atgctctcca ggatggcgcc gatccagtcc atgccgccgt cactgcatgc
     1921 cggacgccag cacgtggcac gctcgctcgc aaggcaagca agctcaggcg ttcacgacct
     1981 cgccgccgtt cgggtggatg acctgcccag acacgtacga gccgtcgtcg ctcgccagga
     2041 acacgaaggc cggggcgacc tcctcgggct ggcccggtcg gcccatcggc gtgtccgcgc
     2101 cgaacgactc gaccttgtcc ggcgggaagg tcgatgggat gagcggcgtc cagatcgggc
     2161 cgggcgcgac ggcgttgacg cggatcttcc tgtcggccag gttgatcgag agcgaacggg
     2221 tgaacgccac gatcgcgcct ttcgtcgatg agtagtcgag cagggtcgga ctcccgcggt
     2281 acgcggtcac cgaggtcgta ttgacgatcg cgcttccctc gccgaggtgc ggcagcgcgg
     2341 ccttcaccag atagaacatc gcgaagatgt tcgtgcggaa cgttcgctcg agctgccgcg
     2401 cggtgatctc gctgatgtcg tccttgggat gctgttccgc cgcgttgttc acgagcacgt
     2461 cgagccggcc cagcgcgcgc accgtccgag cgacggcgcg tttgcagaac gcctcatccc
     2521 cgacgtcgcc cgcgatcgac tcgcagcgcc gtccctcggc ctcgaccaga cgcttcgttt
     2581 ccgcggcgtc ctcgtgctcc tcgaggtaga cgatggcgac gtccgcgccc tccctggcga
     2641 aggcggccgc gacggctcgc ccgatgccac tgtcgcc
//
LOCUS       DASTZR010000401         2388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_22542, whole genome shotgun sequence.
ACCESSION   DASTZR010000401 DASTZR010000000
VERSION     DASTZR010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22542"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..726
                     /locus_tag="VFV20_08925"
     CDS             25..726
                     /locus_tag="VFV20_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HEU5288512.1"
                     /translation="MLGIARPAAAGEGGAAAAALPVAHVDETYPVHVGAALDPGLTRW
                     MWLVKWFLAIPHVVVLAFLWVAVWVLTVVAFFAILFTGRYPRSIFDFTVGVLQWTWRV
                     GFYAFSVLGTDRYPPFTLAPVPEYPATLEVPYPEQLSRGLVLVKWWLLAIPHYIVLGI
                     MFGNPGWVGDVWRIEGPSLNTVLVVIGVVVLLFRGRYPREVFDFVAGMNRWALRVAAY
                     ALLLRDEYPPFRLGD"
     gene            complement(734..1060)
                     /locus_tag="VFV20_08930"
     CDS             complement(734..1060)
                     /locus_tag="VFV20_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288513.1"
                     /translation="MTEREARVARNESRFRYRNEALHRHAGILPGTIAYACECPADDC
                     RELIVLTAEQYESVRADDRQFLVARGHEVPDVEAVVEDNGTWLVVRKLGEAGEVAERL
                     APSPGS"
     gene            1433..>2388
                     /locus_tag="VFV20_08935"
     CDS             1433..>2388
                     /locus_tag="VFV20_08935"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5288514.1"
                     /translation="MVGLACAFVLTGLSLAIPVLIQHAIDDAILKHDREKLLVFLGAI
                     MAIACARFAVNFTRRYATARVGINVEALMRQYMYSAYLRYPRAFYDRHATGEVVSRAT
                     NDLYPIRYFIGWGLIQGTQSLLMIVGASAVLVTVNATLMLYAAVVMPLVGLLAWFFAH
                     KVFPISRRVQEKKAVVTEAADEAVVGIEMVQAFGREDDVRDRFGGRAELLRDETLRQA
                     AIESRYIPGLLFIPTLGIAAVVYFGGKEVIAGTLTLGEFTLFMQLLLMLVWPLEALGW
                     IINLGQRALASASRSFAWLDSIEPLPEPSSPRPLPAGKLAVE"
BASE COUNT          290 a          872 c          823 g          403 t
ORIGIN      
        1 gccggcgccg ccgccggcct gctcgtgctc ggcatcgcgc ggcccgcggc cgccggcgag
       61 ggcggcgccg cggccgcggc gctccccgtc gcgcacgtcg acgagaccta cccggtccac
      121 gtcggcgccg cgctcgaccc cgggctgacg cggtggatgt ggctcgtgaa gtggttcctc
      181 gcgatccccc acgtcgtcgt gctcgcgttc ctctgggtcg ccgtctgggt gctgaccgtg
      241 gtcgccttct tcgcgatctt gttcaccggc cgctacccgc ggtcgatctt cgacttcacc
      301 gtcggcgtcc tgcagtggac gtggcgcgtc ggcttctacg cgttcagcgt gctcggcacg
      361 gaccgctacc cgccgttcac cctcgcgccc gtgccggagt acccggcgac gctcgaggtg
      421 ccgtaccccg agcagctctc gcgcgggctg gtgctcgtca agtggtggct gctcgcgatc
      481 ccgcactaca tcgtgctcgg gatcatgttc ggcaacccgg gctgggtcgg cgacgtctgg
      541 cggatcgagg ggccgagcct gaacacggtg ctcgtggtga tcggcgtcgt cgtgctgctc
      601 ttccgcggcc gctacccgcg cgaggtcttc gacttcgtcg ccggcatgaa ccgctgggcg
      661 ctccgcgtcg ccgcgtacgc gctgctgctg cgggacgagt acccgccgtt ccgcctcggc
      721 gactgatcgc gcgtcaggag ccgggggagg gggcgagccg ctccgcgacc tcgcccgcct
      781 cgccgagctt gcgcacgacg agccaggtgc cgttgtcctc gacgaccgcc tccacgtccg
      841 ggacctcgtg gccgcgcgcg acgaggaact ggcggtcgtc ggcacgcacg gactcgtact
      901 gctccgcggt gagcacgatc agctcgcgac agtcgtccgc cgggcactcg cacgcgtacg
      961 cgatcgtgcc cggcaggatg ccggcgtgcc ggtgcagggc ctcgttccgg tagcggaacc
     1021 ggctctcgtt gcgcgcgacg cgtgcttccc gctctgtcat ccgttgccta ctgtaagcgc
     1081 tggttccccg ggcgcggcac aggggtcctc gatgcgacgc gtctactcct agttgtacgc
     1141 cccggaacgc cggaagcaaa gcgccgaaaa gcgcgtcctg gcgcggtgct aaggttgctc
     1201 cttcggcccg tcgagtaggc gaaaggggaa cgcgatgcgc gtcagcaccg agcagagccc
     1261 gcgcaaccgc gtccccgcgc actagctccc ggccgcacct gcgcacccgt acgaccggag
     1321 ccccttccag gaggccgttc tgacacgtct cgccgtcgat ccgcccgatc tcgacctgaa
     1381 gccaacgatc cgccggctct tcccggtctg gcggacccag tggcgcctgg tcctggtcgg
     1441 gctcgcgtgc gcgttcgtgc tcaccggcct ctcgctcgcg atccccgtgc tgatccagca
     1501 cgcgatcgac gacgcgatcc tgaagcacga ccgcgagaag ctgctcgtct tcctcggcgc
     1561 gatcatggcg atcgcctgcg cgcgcttcgc cgtcaacttc acccgccggt acgccaccgc
     1621 gcgcgtcggc atcaacgtcg aggcgctgat gcggcagtac atgtactcgg cgtacctccg
     1681 ctacccgcgc gcgttctacg accggcacgc gaccggcgag gtcgtctcgc gggcgacgaa
     1741 cgacctctac ccgatccgct acttcatcgg ctgggggctg atccagggca cgcagtcgct
     1801 gctgatgatc gtcggcgcga gcgcggtgct cgtcaccgtg aacgcgacgc tgatgctgta
     1861 cgcggcggtc gtgatgccgc tcgtcggcct gctcgcgtgg ttcttcgcgc acaaggtgtt
     1921 cccgatctcg cggcgcgtgc aggagaagaa ggcggtcgtg acggaggccg cggacgaggc
     1981 ggtcgtgggg atcgagatgg tgcaggcgtt cggccgcgag gacgacgtgc gcgaccgctt
     2041 cggcgggcgc gccgagctgc tgcgcgacga gacgctccgg caggcggcga tcgagtcgcg
     2101 gtacatcccg ggcctgctct tcatcccgac gctcggcatc gcggccgtcg tctacttcgg
     2161 cggcaaggag gtgatcgcgg ggacgctcac gctcggcgag ttcacgctct tcatgcagct
     2221 gctgctgatg ctcgtgtggc cgctcgaggc cctcggctgg atcatcaacc tcggccagcg
     2281 cgcgctcgcc tccgcgagcc ggagcttcgc gtggctcgac tcgatcgagc cgctgcccga
     2341 gccgtcgagc ccgcggccgc tcccggccgg gaagctcgcg gtcgagtt
//
LOCUS       DASTZR010000402         2965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_962558, whole genome shotgun sequence.
ACCESSION   DASTZR010000402 DASTZR010000000
VERSION     DASTZR010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962558"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1786
                     /gene="alaS"
                     /locus_tag="VFV20_08940"
     CDS             <1..1786
                     /gene="alaS"
                     /locus_tag="VFV20_08940"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU5288515.1"
                     /translation="IAPSNEWRGYVLRRLIRRAALHGRRLGIKTGFLPMLGAEVVKTM
                     QKHHHELTSARDRIAELLTDEEAKFERTLANGLALLNEAVDRAKASGQSFIDGDTVFR
                     LYDTYGFPREMTEEIAGGAGLTIDDRAFHELLDAQRSRSRASAKFTQDAMKFGQFYAM
                     LKESEHLKSEFRGYEELASDARIVSVVVNGERVTQASEGTDVELVLDRTPFYPEGGGQ
                     VGDRGTITSDAGRALVADTQTAAPGVIVMSAKVVDGVLRLGAPARAVVDEQLRRDTMR
                     NHTATHLLHATLRRLFGEDVHQAGSLVHAPNLRFDFTFGRGLTSEELQRVEDEVNRAI
                     LDNADVQAHVMPLQDALASGAMALFGEKYDDVVRVVEAGPSRELCGGTHCHCTGDIGS
                     FFITKEESIGAGVRRIEAVTGMGAIREVRDIRDRMNRAGGLLRVPASRVPDAVSQLLE
                     QREKLQHELEAQQRSGIEAAASGLLAKAEVIGAAKLVAANVGDGGVDQLRALSDRIRS
                     AIGSGVVVLGGVRTGNPFLVISVTKDLIPPLDADKLIKQVQGIIDGKGGGKAESASAG
                     GKDASRLAEAIEAARSTVRERLNGSDRN"
     gene            1809..2864
                     /locus_tag="VFV20_08945"
     CDS             1809..2864
                     /locus_tag="VFV20_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288516.1"
                     /translation="MRPAATRGPAVAGVDLSAGTLHVVVARKEEGRLRVVGRGESPLP
                     DSAISGGLVADRNAAADGLRTAFAVAEHAQRADRVIVALDSDDVRTFHALTSFEREDS
                     RSPVNAGEESRAVREAAADAAQRALAVLEDDPALRGVPTAQLHDDVAALALDGRPLRS
                     LVGHRGRLVEVWTDVTLAPLVLTGAATATLEAARRRGNVVSAAYALGRLLAGSGISDA
                     GVIRLGSDATSLAILREGRVAATRVFALGRDSLAARATRADEDARVWADCVVASLRGL
                     DGPPPERWIFVGVPESLLALPRALGDVVGGIRGGAVDIAPLSVSLATRVFADVPLRSD
                     DLVAAGAAALAAGVYES"
BASE COUNT          421 a         1035 c         1105 g          404 t
ORIGIN      
        1 catcgcgccg tcgaacgagt ggcgcgggta tgtgctgcgc cgcctcatcc gtcgcgccgc
       61 cctccacggc cggaggctcg ggatcaagac cggcttcctg ccgatgctcg gcgccgaggt
      121 cgtcaagacg atgcagaagc atcatcacga gctgacctcg gcgcgcgacc ggatcgccga
      181 actgctgacc gacgaggagg cgaagttcga gcgcaccctc gcgaacggtc tggccctcct
      241 gaacgaggcg gtggatcgcg cgaaggcctc gggccagtcg ttcatcgacg gcgacaccgt
      301 gttccgcctg tacgacacct acggcttccc gcgcgagatg accgaagaga tcgcgggcgg
      361 cgcgggtctg acgatcgacg atcgcgcttt ccacgagctg ctcgacgcgc agcgcagccg
      421 gagccgcgcg agcgccaagt tcacgcagga tgcgatgaag ttcggacagt tctacgcgat
      481 gctgaaggag agcgagcacc tgaagagcga gttccgcggg tacgaggagc tcgccagcga
      541 cgccaggatc gtgtcggtcg tggtgaacgg cgagcgcgtc acgcaggcct cggagggtac
      601 cgatgtcgag ctcgtgctcg accgcacgcc gttctatccg gaaggcggcg gtcaggtggg
      661 cgaccgcggc acgatcacga gcgacgcggg gcgtgcccta gtcgcggaca cgcagaccgc
      721 ggcgcccggc gtgatcgtga tgagcgccaa ggtcgtcgac ggcgtgctgc ggctcggcgc
      781 cccggcgcgc gcggtcgtcg acgagcagct ccgccgcgac acgatgcgca atcacaccgc
      841 gacgcacctg ctgcacgcca ccctgcgccg gctcttcggc gaggacgtcc atcaggccgg
      901 ctccctcgtc catgcgccaa accttcgttt cgacttcacg ttcggtcgtg gattgacgtc
      961 cgaggagctg cagcgcgtcg aggacgaggt gaaccgcgcc atcctcgaca acgccgacgt
     1021 ccaggcgcac gtcatgccgc tgcaggacgc gctcgcctcg ggcgcgatgg cgctcttcgg
     1081 cgagaagtac gacgatgtcg ttcgcgtggt ggaggccgga ccgagccgcg agctctgcgg
     1141 cggcacgcac tgccattgca ccggcgacat cgggtccttc ttcatcacga aggaggagag
     1201 catcggcgcg ggcgtccggc gcatcgaggc ggtcaccggc atgggagcca tccgcgaggt
     1261 gcgtgacatc cgggaccgca tgaaccgcgc gggcggcctg ctgcgcgtgc cggcgtcgcg
     1321 ggtaccggac gcggtctcgc agctcctcga gcagcgcgag aagctccagc acgagctcga
     1381 ggcgcagcag cgcagcggga tcgaggccgc ggcgtccggg ctcctggcga aggccgaggt
     1441 catcggtgcc gcgaagctcg tcgcggcgaa cgtcggcgat ggcggcgtcg accagctgcg
     1501 cgcgctgtcg gaccgcatcc ggtcggcgat cggctcgggg gtcgtggtcc tgggtggtgt
     1561 gcggacaggg aaccccttcc tcgtgatctc ggtcacgaag gatctgatcc cgccgctcga
     1621 cgcggacaag ctcatcaagc aggtgcaggg gatcatcgac gggaagggcg gcggaaaggc
     1681 ggagagcgct tccgcaggcg gcaaggacgc gtctcgcctc gccgaggcga tcgaggcggc
     1741 acggtccacg gtccgcgaac gtttgaacgg aagtgaccgc aactagaccc gccacccaca
     1801 gccgtgacgt gcgtccggcc gcgacgcgcg gcccggcggt ggctggcgtc gatctgtcgg
     1861 ccggcaccct ccacgtcgtc gtcgcgcgca aggaggaggg gcgcctgcgt gtcgtgggac
     1921 gaggcgagag cccgcttccc gacagcgcga tctccggcgg cctcgtcgcc gaccgcaacg
     1981 cggccgccga cgggctgcgc acggcgttcg cggtcgccga gcacgcgcag cgcgccgacc
     2041 gcgtcatcgt cgccctggac agcgacgacg tccggacgtt ccacgcgctc acgtcgttcg
     2101 agcgcgagga ctcgcgctcg ccggtgaatg cgggcgagga gtcgcgcgcg gtgcgcgagg
     2161 cagcggccga cgcggcccag cgagcgctgg cggtcctcga ggacgatccg gcgctccgcg
     2221 gcgtgcccac ggcgcagctc cacgacgacg tcgcggcgct ggcgctcgat ggccggccgc
     2281 tccgttcgct ggtcggacat cgcggccgtc tcgtcgaggt gtggaccgac gtgaccctcg
     2341 ctccgctcgt cctgaccggc gcggcgacgg cgacgctcga ggcggcgcgc aggcgcggca
     2401 atgtcgtgtc ggcggcgtac gccctcggac ggctgctcgc cggatccggg atcagcgacg
     2461 ccggggtcat ccgcctcggg agcgacgcca cgtcgctcgc gatcctccgc gaaggccggg
     2521 tcgccgcaac gcgcgtgttc gcgctcggtc gcgacagtct cgccgcccgc gccacgcggg
     2581 cggacgagga cgcccgcgtg tgggcggact gcgtcgtcgc ctcgctgcgc ggcctcgacg
     2641 gcccgccacc agagcgctgg atcttcgtcg gcgtgccaga gtcgctgctc gccctgccac
     2701 gcgccctcgg cgacgtggtg ggcgggatcc gcgggggcgc ggtggacatc gcgccactct
     2761 cggtgagcct cgcgacgcgc gtcttcgcgg acgttccact gcgttccgac gatctcgtcg
     2821 cggcgggcgc ggccgcgctc gccgccgggg tgtacgagtc gtgacggtgc aggtggcggg
     2881 ccgctcggtc atcgacctcg gcgcgcgggc gacgatcctc gaggccatgg accgcgtgcg
     2941 cgcggcgggc acggccgacg aggtc
//
LOCUS       DASTZR010000403         7061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_408908, whole genome shotgun sequence.
ACCESSION   DASTZR010000403 DASTZR010000000
VERSION     DASTZR010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7061
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408908"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="VFV20_08950"
     CDS             complement(<1..1193)
                     /locus_tag="VFV20_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288517.1"
                     /translation="MYRAVHADRGGRILVTDHPAYAFDGVRPVPLTDALPLPADASVV
                     AIEREGIASERSGKPRRLGAGRLAAAAILPPGYLRTQLPAYVDATDRAELAPRPYAAV
                     AADENGALVVAALRLDSDATHDAAAYGRADVARHVDAGLRTHPGDRLVRQLARCAREY
                     GCRAAANAFFGRWECALPIAAPANERPPAATLPRRDGEAEPTEPAAFHPSTDEVAGLA
                     TEHLKAGGTLLSFGRACEGEPLLAVREVEDAIRRIRAETDEGTIHLETNGSVAAGLRR
                     LASAGLESVAVRMVSARAATYEVMHGPDGYRLPDVRASLRVLAELRISTSVLILVLPG
                     LFDRPVEQDALIAVLAELPEGSSLLLRDLHADPLRALALVADRTAEPTGVAAAVEHIR
                     HELPHL"
     gene            complement(1244..2251)
                     /locus_tag="VFV20_08955"
     CDS             complement(1244..2251)
                     /locus_tag="VFV20_08955"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEU5288518.1"
                     /translation="MLFALRIPAAAGAVYGPRGLRFLLNVATALLVVSLPIAASTATT
                     ATIAPEFPTVAAVPSVATEDGSSLRVQSAARGVTAASRSVVTVAAEDVRPVVQYQIAD
                     GDNLGSIAKQYQVELDDLAYANGIDDEGARLHIGNTLLIPPGRGALYFVKDGDTVASV
                     AAKFKVDATAIMSYNRLYFEPEHFATDQLIFVPGAEVPAMKRTEIAKTYTPSNGQLPA
                     RTGRLGWPVNGVITQYFWWGHTGVDIAAPYGTTIVASDDGVVSATGWVAVGGLRVCVD
                     HSGGLQTCYYHTGAVFVTPGQTVARGQPLASIGMTGVTTGPHVHWEVKLNGVAVNGLA
                     Y"
     gene            2574..2939
                     /locus_tag="VFV20_08960"
     CDS             2574..2939
                     /locus_tag="VFV20_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEU5288519.1"
                     /translation="MPDYNLSYVYVFAFFVVGALLVTVIMLLSHLLSPWKPTREKLRT
                     YESGEEPVGQAWGRYPTHFYVFALLFVVFDVEVIFLFPWAVLFRDLGWFGFGEMVVFI
                     AILVVGLFYAWRKKALNWY"
     gene            2930..3418
                     /locus_tag="VFV20_08965"
     CDS             2930..3418
                     /locus_tag="VFV20_08965"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEU5288520.1"
                     /translation="MVLSSPRPTIDLAKARDELAARLGDRARVSILHDMLVVDVDRAR
                     LIEVARIVRDEAPTRCDLYSVNAGVDTGSEFRSVTFVRGTKTHVFVMLRTPCTELDPH
                     VPSLVPVWEGANWPERETYDMFGIEFDGHPDLRRVFLEESFPGHPLRKSFLPPRSDAR
                     GG"
     gene            3426..4592
                     /gene="nuoH"
                     /locus_tag="VFV20_08970"
     CDS             3426..4592
                     /gene="nuoH"
                     /locus_tag="VFV20_08970"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEU5288521.1"
                     /translation="MPVGPFGIDPAAWAVIRLVLAIVGGLFLVFNGALAQIYLERKIQ
                     AIIQDRVGPIHTGPWGLLQTVADAIKLLGKEDIRARLTDRWFFLAAPAIVFSPMLASY
                     AVLPFGESIVGADLNIGLLYLTTLGSMTVLGIVIAGWASNNKYSLIGGLRSAGQLISY
                     EVPQILSLVTVVLVVGSLSLTHIVDAQPDFWINALILPLALVTYFIAGLAETNRNPFD
                     LPEAESELVAGFLTEYSGMRWGLFFVAEYGEVTVVSAIVTTLWFGGWHGPLTDAHPVI
                     GPLLGVLWFTLKTYFFVLVFMWIRATLPRFRIDQLMSLCWKFLIPVTLTNLLVTAALV
                     LVFADARIPIAIANWVMLAIVIAAVPFFQRRRLAALRRRLEARRPVLASAERAG"
     gene            4599..5126
                     /locus_tag="VFV20_08975"
     CDS             4599..5126
                     /locus_tag="VFV20_08975"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEU5288522.1"
                     /translation="MSFDFVQVLLFALLALGILGAAAGVVLVNRVTAAALLMAFTFLC
                     NAGMFVLLGAETVAAFQVLIYVGAITVLILFGVMFTPQHDRPYALFFQKQTPIAAVFV
                     APIGIAVALLSFSFTDGGKATPGGGDLEPLATSVFRDYAFPFEIASVLLLAAMIGAIV
                     LVKRDDAESPTSDDE"
     gene            5123..5437
                     /gene="nuoK"
                     /locus_tag="VFV20_08980"
     CDS             5123..5437
                     /gene="nuoK"
                     /locus_tag="VFV20_08980"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEU5288523.1"
                     /translation="MSVVPIGPFIAVSALLFFIGVAGVLVRRNAVVILMCVELMLNAT
                     NLALVAFSRRAGLADERGSVFVVFIFVIAAAEVAVGLAIVLSAYRNRPTVDVDDIHAM
                     KG"
     gene            5442..>7061
                     /gene="nuoL"
                     /locus_tag="VFV20_08985"
     CDS             5442..>7061
                     /gene="nuoL"
                     /locus_tag="VFV20_08985"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEU5288524.1"
                     /translation="MIANVWLVPAFPLAAFLINGLFGRRWLGKMTGWIATAAIALSAV
                     VAVGVFLEILGGKERTTVTLYEWMGVGDFHVNIAALVDPLSSVMLLVVTIVGTLIFIY
                     ANGYMAHDHGIHRFFTWFPLFAFAMLILVMADNYLLMFVGWEGVGLCSYLLIGFWFDK
                     PAPQHAANKAFWMNRIGDWGYTIGMVTTFLVFGSMTFTEVFEKVDIATQANLTLIAIA
                     FFVGATGKSAQLPLYSWLPDAMEGPTPVSALIHAATMVTAGVYLVARSTPIFVAAGPA
                     LQVVAVVGAVTALFAATIALVQFDIKRVMAYSTVSQLGYMFLALGVGAPAAAIFHLAT
                     HAFFKALLFLGSGSVIHGMQGEQDMRKMGGLRRKMPVTYWTMLIAGGALAALPPLAGF
                     WSKDEIVGAAWNSGQTALWAVGIFTAILTAFYVTRAMWMTFHGEPREHHLYDHAHESP
                     AVMTLPLIALAVGSATLGIVIGFPPDAGSIHSFLRPVLEVEGAAEHAPELATILALAA
                     VSVGAGILGILIGLSMYVRHRPDPSAVARAFG"
BASE COUNT          952 a         2439 c         2412 g         1258 t
ORIGIN      
        1 aggtggggga gctcgtgccg gatgtgctcg accgcggccg cgacgccggt gggctcggcg
       61 gtgcggtccg cgacgagcgc gagcgcccgg agcggatcgg cgtgcaggtc gcgcagcagg
      121 agcgagctgc cctcgggcag ctccgcgagc acggcgatga gcgcgtcctg ctcgacgggc
      181 cgatcgaaca ggccgggcag gacgaggatc agcacgctcg tggagatccg aagctcggcg
      241 agcacgcgca gcgacgcgcg cacgtcgggg aggcggtacc cgtcggggcc atgcatcacc
      301 tcgtaggtgg cggctcgggc ggacaccatg cggaccgcga cgctctcgag cccggcggat
      361 gcgagcctgc gcagtcccgc cgccaccgat ccgttcgtct cgaggtggat cgtcccctcg
      421 tcggtctcgg cgcggatacg acggatcgcg tcctcgacct cgcgcaccgc gagcaggggc
      481 tcgccctcgc aggcgcgacc gaacgagagg agcgttccgc ccgccttcag gtgctctgtc
      541 gcgaggcccg cgacctcgtc cgtggacgga tggaacgccg cgggctcggt gggctcggcc
      601 tcgccgtcgc gccgcggcag ggtcgccgcg ggcgggcgct cgttggctgg cgcggcgatc
      661 gggagcgcgc actcccaacg accgaagaac gcgttcgccg ccgcacggca accgtactcg
      721 cgcgcgcagc gcgcgagctg gcgcacgagg cggtcgccgg gatgcgtccg caggccggca
      781 tcgacgtgcc gcgcgacgtc ggcgcgccca tacgcggctg cgtcatgcgt cgcgtcgctg
      841 tcgaggcgca gtgccgcgac gacgagcgcg ccgttctcgt cggccgcgac ggcggcgtag
      901 gggcgtggtg cgagctcggc gcggtcggtc gcgtcgacgt acgcgggcag ctgcgtgcgg
      961 aggtagccgg gaggaaggat cgcggctgcg gcgagccggc ccgcaccgag tcgacgcggc
     1021 ttgccgctgc gctcggacgc gatcccctcg cgctcgatcg cgacgacgct cgcgtccgcg
     1081 ggcagcggga gcgcgtccgt caacggcacg gggcgcacac catcgaaggc gtaggcgggg
     1141 tggtcggtga cgaggatccg tccgccgcga tcggcgtgga ccgcgcgata catgactaga
     1201 gcgtagcgag ggaccggcga cgcgccgggg ctacgagacc gggctagtac gcgagcccgt
     1261 tgaccgcgac tccattgagc ttcacttccc agtggacgtg agggcccgtc gtgacgcccg
     1321 tcatgccgat cgatgcgagc ggctggccac gggcgaccgt ctgtccgggt gtcacgaaga
     1381 cggcgccggt gtggtagtag cacgtctgaa ggccgccgga atgatcgacg cagacgcgca
     1441 gtccgccgac cgcgacccag cccgtcgcgc tcacgacacc gtcgtccgac gccacgatcg
     1501 tcgtgccgta gggcgcggcg atgtcgacgc cggtgtgtcc ccaccagaag tactgcgtga
     1561 tcacaccgtt caccggccag ccgagacggc cggtacgtgc cggcagctgt ccgttcgacg
     1621 gcgtgtaggt cttcgcgatc tcggtgcgct tcatcgccgg cacctcggcg ccggggacga
     1681 agatgagctg atcggtcgcg aagtgctccg gctcgaagta gaggcggttg taggacatga
     1741 tcgcggtcgc gtcgaccttg aacttcgcgg cgacgctggc gacggtgtcg ccatccttca
     1801 cgaagtagag agcgccccgg cccggcggga tgagcagggt gttgccgatg tggagtctgg
     1861 cgccctcatc gtcgatgccg ttggcgtatg cgagatcgtc gagctcgacc tggtactgct
     1921 tcgcgatcga cccgaggttg tcgccgtcgg cgatctggta ttgcacgacc ggccgcacgt
     1981 cctcggcggc gaccgtgacg acgctccgcg acgcggcggt cacaccgcgg gcggccgact
     2041 gcacacgcag gctcgagccg tcttccgtcg cgacgctcgg gaccgcggcg accgtgggga
     2101 actccggtgc gatcgtcgcg gtcgtcgcgg tcgatgcggc gatcggcaat gagacgacga
     2161 ggagcgctgt ggcgacgttg agaaggaagc ggagtccgcg cgggccgtac acggcgccgg
     2221 ctgcggctgg gatgcggaga gcgaagagca cgagcgctgc tctcgcgaga tctttggcgg
     2281 tgtggccgac acgctctcgc gcctgtcgtg ccgcgcgccg cgcctgaagg cgcggcgacg
     2341 gcgtggcctg cgcgcgccgg ctttgtgccg tcagcaagat ccccagaacc ctccccgaag
     2401 cgatcagcct gtacgcaatc cgtactggca gtcggcagtg accgcaccgt acccgcgctt
     2461 ccgcgtatgt gtcaaccctt acgttttcgt cacacttcga ccacaactcg gtcagccaga
     2521 cgcgattttc ggtgcgtttg cggtcgttct gggccgtccc tacgctaacg cgagtgcccg
     2581 actacaacct ctcctatgtc tacgtgttcg cgtttttcgt ggtcggagcc cttctcgtga
     2641 ccgtgatcat gctgctctcc cacctgctct ccccgtggaa gccgacccga gagaagctgc
     2701 gtacgtacga gtcgggggag gagccggtgg gccaggcatg gggccgctat ccgacgcact
     2761 tctacgtgtt cgcgctgctg ttcgtcgtct tcgacgtcga ggtgatcttc ctcttcccgt
     2821 gggccgtgct cttccgcgat ctcggctggt tcggcttcgg cgagatggtg gtgttcatcg
     2881 ccatcctcgt cgtgggtctc ttctacgcgt ggcgaaagaa ggccctgaat tggtactgag
     2941 ctcgccccgg ccgacgatcg atctcgcgaa ggcgcgggac gagctcgcgg cgcgtctcgg
     3001 cgaccgcgcg cgcgtctcga tcctccacga catgctcgtg gtggacgtgg accgcgcccg
     3061 gctcatcgag gtcgcgcgga tcgtgcgtga cgaggcgccg acacgttgcg acctgtactc
     3121 ggtgaacgcc ggtgtggaca ccggcagcga gttccgcagc gtgaccttcg tgcgagggac
     3181 gaagacgcac gtcttcgtga tgttgcgcac gccatgcacc gagctcgacc cgcacgtgcc
     3241 gtcgctcgtg ccggtctggg agggcgcgaa ctggccggaa cgcgagacat acgacatgtt
     3301 cggcatcgaa ttcgacggcc atcccgatct ccgacgtgtg ttcctcgagg aatcgttccc
     3361 cggccatccg ctccggaagt cgttccttcc gccgcgcagc gacgcgcggg gcggttgagc
     3421 tgaccatgcc cgtcggcccg ttcggtatcg accccgcggc ctgggccgtc atccgtctcg
     3481 tcctcgcgat cgtcggaggg ctcttcctcg tcttcaacgg cgcgctcgcg cagatctacc
     3541 tcgagcggaa gatccaggcg atcatccagg accgcgtcgg accgatccac accgggccgt
     3601 ggggtctgct ccagaccgtc gccgatgcca tcaagctgct cggcaaggag gacatccgcg
     3661 ctcggctcac ggaccggtgg ttctttctcg cggcgcccgc gatcgtgttc agcccgatgc
     3721 tcgcgtccta cgccgtgctg ccgttcggcg agagcatcgt gggcgccgac ctcaacatcg
     3781 gcctgctgta cctgacgacg ctcgggtcga tgacggtgct cggcatcgtc atcgcgggct
     3841 gggcgtcgaa caacaagtac tcgctcatcg gcggcctgcg ctccgcgggc cagctcatct
     3901 cgtacgaggt gccgcagatc ctctcgctcg tcaccgtcgt gctcgtcgtc ggctcgctgt
     3961 ccctcacgca catcgtcgac gcgcagccgg acttctggat caatgcgctg atccttccgc
     4021 tggcgctcgt cacgtacttc atcgccggcc tggctgagac aaatcgaaac ccattcgatc
     4081 tccccgaggc cgagtcggag ctggtcgccg gcttcctcac cgagtactcg ggcatgcgct
     4141 ggggtctctt cttcgtcgcc gaatacggtg aggtgacggt ggtgtccgcg atcgtgacca
     4201 cgctctggtt cggtggctgg cacggcccgt tgacggacgc acacccggtg atcggcccgc
     4261 tgctcggcgt gctctggttc acgctgaaga cgtacttctt cgtcctggtg ttcatgtgga
     4321 tccgagccac cctgccgcgg ttccggatcg accagctgat gtcgctgtgc tggaagttcc
     4381 tgatcccggt gacgctcacg aacctgctcg tcaccgcggc gctggtgctc gtgttcgcgg
     4441 acgcgcgcat cccgatcgcg atcgcgaact gggtgatgct cgccatcgtc atcgccgcgg
     4501 tgccgttctt ccagcggcgg cgtctcgccg cgctccggcg ccggctcgag gcgcggcgac
     4561 cggtcctcgc gtccgctgag cgggctggat agcctcggat gtcgttcgac ttcgtccagg
     4621 tcctcctgtt cgccctgctc gcactcggca tcctcggcgc cgcggccggc gtcgtgctcg
     4681 tgaaccgcgt caccgccgcc gcactgctga tggcgttcac gttcctctgc aacgcgggga
     4741 tgttcgtcct gctcggcgcc gagaccgtcg ccgcgttcca ggtgctcatc tacgtcggcg
     4801 cgatcaccgt cctgatcctc ttcggtgtca tgttcacgcc gcagcacgac cgtccctacg
     4861 cgctgttctt ccagaagcag acgccgatcg ccgccgtctt cgtggcgccc atcgggatcg
     4921 ccgtggcgct gctctcgttc tcgttcacgg atggcgggaa agcgacgccc ggcggtggcg
     4981 acctggagcc gctggcgacg agcgtgttcc gcgactacgc gttcccgttc gagatcgcgt
     5041 ccgtcctgct cctcgcggcg atgatcggcg cgatcgtcct ggtgaagagg gacgacgccg
     5101 aatccccgac atcggacgac gaatgagcgt cgtcccgatc ggcccgttca tcgccgtgtc
     5161 cgcgctcctc ttcttcatcg gtgtcgcggg cgtgctcgtc cgccgcaacg ccgtcgtgat
     5221 cctcatgtgc gtcgagctga tgctcaacgc gacgaacctc gcgctcgtgg cgttctcgcg
     5281 gcgggccggc ctcgccgacg agcgcggcag cgtcttcgtc gtgttcatct tcgtcatcgc
     5341 cgccgcggag gtggccgtgg gtctggcgat cgtgctctcc gcgtaccgca accggccgac
     5401 ggtagacgtg gacgacatcc acgcgatgaa gggataggcc gatgatcgcg aacgtctggc
     5461 tcgtcccggc cttcccgctc gccgccttcc tgatcaacgg gctcttcggg cgccgctggc
     5521 tcggcaagat gaccggatgg atcgccaccg cggcgatcgc gctctcggcc gtcgtggccg
     5581 tgggtgtctt cctcgagatc ctcggcggca aggagcgcac gaccgtcacg ctctacgagt
     5641 ggatgggcgt gggcgacttc cacgtcaaca tcgcggcgct cgtggatccg ctctcgagcg
     5701 tgatgctgct cgtcgtcacg atcgtcggga cgctcatctt catctacgcg aacggctaca
     5761 tggcgcacga ccacggcatc caccgcttct tcacgtggtt cccgctcttc gcgttcgcca
     5821 tgctcatcct ggtcatggcc gacaactacc tcctcatgtt cgtggggtgg gagggcgtcg
     5881 gcctctgctc gtacctgctg atcggcttct ggttcgacaa gcccgcgccg cagcacgcag
     5941 cgaacaaggc gttctggatg aaccgcatcg gcgactgggg ctacacgatc gggatggtca
     6001 ccaccttcct cgtcttcggc tcgatgacgt tcaccgaggt cttcgagaag gtcgacatcg
     6061 cgacgcaggc caacctcacg ctcatcgcga tcgcgttctt cgtcggcgcg acgggaaagt
     6121 ccgcgcagct gccgctctac tcgtggctgc cggacgcgat ggaggggccg acgccggtgt
     6181 ccgcgctcat ccacgcggcg accatggtga ccgccggcgt gtacctggtc gcgcgcagca
     6241 cgccgatctt cgtcgcggcc gggccggccc tgcaggtcgt cgcggtcgtc ggcgcggtca
     6301 ccgcgctgtt cgccgcgacc atcgcgctcg tgcagttcga catcaagcgc gtgatggcgt
     6361 attcgacggt gagccagctc ggctacatgt tcctcgcgct cggcgtcggc gcgccggctg
     6421 cggcgatctt ccacctggcg acccacgcgt tcttcaaggc gctcctcttc ctcggatccg
     6481 gctctgtcat ccacgggatg cagggcgagc aggacatgcg gaagatgggc gggctgcgtc
     6541 gcaagatgcc ggtgacgtac tggacgatgc tcatcgccgg cggcgcgctg gccgcgctgc
     6601 ccccgctcgc gggcttctgg tcgaaggacg agatcgtcgg cgcggcgtgg aacagcgggc
     6661 agaccgcgct gtgggcggtc ggcatcttca ccgcgatcct cacggcgttc tacgtgaccc
     6721 gcgcgatgtg gatgacgttc cacggtgagc cgcgcgagca ccacctctac gaccacgcgc
     6781 acgagtcacc cgcggtcatg acgcttccgc tgatcgcgct cgcggtgggc agcgcgacgc
     6841 tcggcatcgt gatcggcttc ccgccggatg cgggatccat ccactccttc ctgcgccccg
     6901 tcctcgaggt cgaaggcgcg gccgagcacg cgcccgagct cgccacgatc ctcgcgctcg
     6961 ccgcggtctc cgtgggcgcc ggcatcctcg gcatcctcat cgggctgtcg atgtacgtgc
     7021 gccatcgccc cgatccgtcg gcggtcgcgc gcgcgttcgg t
//
LOCUS       DASTZR010000404         2862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_284053, whole genome shotgun sequence.
ACCESSION   DASTZR010000404 DASTZR010000000
VERSION     DASTZR010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284053"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /gene="aroA"
                     /locus_tag="VFV20_08990"
     CDS             <1..1093
                     /gene="aroA"
                     /locus_tag="VFV20_08990"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU5288525.1"
                     /translation="DAIASLGHEVRWADGRIEIAPREVPYEHAAIDARDAGTVARFAT
                     ALAATTGREVRVDGSARMRERPMRALVSALRSLGATIDTDALPLTVRGPLVGGQVQVP
                     GYESSQFASALLLVAPRMQNGLRLLLTGGLVSAPFLEMTVASLRARGVRVQRPSARVF
                     VVRPQPIEGRTVEIPGDVSAATYPAAAAAILGGSVTIENASARRATGGQGDARFFELI
                     ARMGCAVTRGGGSVTVRRTGELLGLVANVSDCSDVFPTLAAIATQTVTPTELGGLGHT
                     RGQESDRIAAVAAAISALGGHAEAYGDAIRIEPRPLHEGVVDAAGDHRIAMAFSILGL
                     LVPGVAIAGASSVSKTFPGFYEMLEDLKR"
     gene            complement(1112..1924)
                     /locus_tag="VFV20_08995"
     CDS             complement(1112..1924)
                     /locus_tag="VFV20_08995"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEU5288526.1"
                     /translation="MRIGVAGLGLIGGSLALALRERHDIAAFDVDRATREAARARGLT
                     AVDDLEALLPSDAVIVATPMASVLPTLAALAPRADGAVLLDVSSVRGPVDALARDTTS
                     ARIVGMHPMAGRSARGFASADPALLAGRPFLVVPTASADSDAMAVAGGVARDAGGVVT
                     VCSAAEHDRIVAVTSALPLAVAAALAIAGAEATPQLAAFAGPGLRDATRLADTPVDLA
                     EALLLANGGNVVAAFARIRALLDELERAVAERDAAAVRATLERAASARALLG"
     gene            complement(1921..>2862)
                     /gene="aroF"
                     /locus_tag="VFV20_09000"
     CDS             complement(1921..>2862)
                     /gene="aroF"
                     /locus_tag="VFV20_09000"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU5288527.1"
                     /translation="RSTGASRIVLGVVGGGKVDPRLIELLPGVHEVVRITQPYKLASR
                     TFKPEDTVITIGDLRIGGDEVIVIAGPCSAESEAQVHATAAAVKRAGAKVLRGGAFKP
                     RSSPYTFRGLGVDGLELLAEARAETGLAIVTEVLTPGDVETVATYADCLQIGARNMQN
                     YALLDAVGEQAKPVLLKRGIAATIEELLLSAEYVLARGNRDVIICERGIRTFEKYTRN
                     TFDVNAIPLLKRLTHLPVVGDPSHGTGKWYLVAPVALAAVAAGADALMIEVHPSPDHA
                     LSDGFESLTFENFAALMSQVGAVGRAVGRSLAEGVRV"
BASE COUNT          393 a         1067 c         1032 g          370 t
ORIGIN      
        1 cgacgcgatc gcgtcgctcg gccacgaggt ccgctgggcg gatggccgga tcgagatcgc
       61 accgcgggag gtcccgtacg agcacgcggc catcgacgcg cgcgacgcgg gaacggtcgc
      121 acgcttcgcc accgcgctcg cggccacgac gggccgcgag gtgcgcgtcg atggctcggc
      181 gcggatgcgc gagcgcccga tgcgtgcgct cgtcagcgcg ctgcgctcgc tcggcgccac
      241 gatcgatacg gacgcgctcc cgctcacggt ccgcgggccg cttgtcggcg gccaggtaca
      301 ggtccccgga tacgagtcga gccagttcgc gtccgcgctg ctgctcgtcg cgccgcgcat
      361 gcagaatggc ctgcgcctgc tgctcaccgg cggcctcgtc tcggcaccct tcctcgagat
      421 gaccgtcgcg tcgctgcgcg cgcgcggcgt ccgggtgcag cggccctccg cgcgcgtctt
      481 cgtcgtgaga ccgcagccga tcgaaggccg cacggtcgag atccccggcg acgtcagcgc
      541 cgcgacctat cccgcggcgg ccgccgcgat cctcggcggc agcgtgacga tcgaaaacgc
      601 ctcggcgcgg cgagcgaccg gcgggcaggg cgatgcgcgc ttcttcgagc tcatcgcgcg
      661 catgggctgc gcggtgacgc gcggcggcgg ctccgtgacg gtgcggcgca ccggagagct
      721 cctggggttg gtcgcgaacg tgtccgactg ctcggacgtg ttccccaccc tcgccgcgat
      781 cgccacccag acggtgacac cgaccgagct tggaggcctg ggccacacgc gcggccagga
      841 gtccgatcgc atcgccgcgg tcgcggccgc gatcagcgcc ctgggcggcc acgccgaggc
      901 gtacggcgac gcgatccgca tcgagccgcg gccgctccac gaaggcgtgg tcgacgccgc
      961 cggcgaccac cgcatcgcca tggcgttctc gatcctcggc ctgctggtcc cgggcgtcgc
     1021 gatcgccggt gcgagctctg tgtcgaagac gttccccggc ttctatgaaa tgttggagga
     1081 cctgaagcga tgagcgcccg gagccgaccg cctagccgag cagcgcgcgc gctgacgcgg
     1141 cgcgctcgag cgtcgcgcgc accgcggcgg catcgcgctc cgcgaccgca cgttcgagct
     1201 cgtcgagcag cgcgcggatc cgcgcgaacg ccgccacgac gttcccgccg ttggcgagca
     1261 gcagcgcctc ggcgagatcg acgggcgtgt cggcgagcct cgtcgcgtca cgcagtccgg
     1321 gaccggcgaa cgccgcgagc tggggcgtcg cctccgcgcc ggcgatcgcg agcgccgcgg
     1381 cgaccgccag cggcagcgcc gaggtgaccg cgacgatgcg gtcgtgctcg gcggccgagc
     1441 acaccgtcac gacgccaccc gcgtcgcgcg cgacgccacc ggcgacggcc atcgcatcgc
     1501 tgtccgcgga cgccgtcggc acgacgagga acggacggcc cgcgaggagc gccggatccg
     1561 cgctggcgaa tccgcgtgcg ctccgcccgg ccatgggatg catgccgacg atgcgcgccg
     1621 atgtcgtgtc gcgggcgagc gcgtccaccg gaccgcggac cgacgaaacg tcgagcagca
     1681 cggcgccgtc ggcgcgcggg gccagggcgg ccagtgtcgg caggacgctc gccatcggcg
     1741 tcgcgacgat gaccgcgtcg gacggaagga gcgcttccag atcgtccacc gccgtgaggc
     1801 cgcgggcgcg cgccgcctcc cgtgtcgcac gatcgacgtc gaacgccgcg atgtcgtgac
     1861 gctcccgcag cgccagcgcg agcgagccgc cgatcaggcc caatccggcg accccgatcc
     1921 tcatacgcgc acgccctcgg cgagagaccg cccgaccgcg cggccgaccg ccccgacctg
     1981 cgacatgagg gccgcgaagt tctcgaacgt cagcgactcg aacccgtcgg acagcgcgtg
     2041 atccgggctc ggatggacct cgatcatgag cgcgtcggcg ccggccgcca ccgccgccag
     2101 cgcgaccggg gcgacgaggt accacttccc ggtgccgtgc gagggatcgc ccacgacggg
     2161 caggtgcgtc agccgcttga gcagcgggat cgcgttcacg tcgaaggtgt tgcgggtgta
     2221 cttctcgaac gtgcggatgc cgcgctcgca gatgatcacg tcacgattgc cgcgggccag
     2281 gacgtattcg gcggagagca gcagctcctc gatcgtcgcg gcgatgcccc gcttcagcag
     2341 cacgggcttc gcctgctcgc ccaccgcgtc gagcagcgcg tagttctgca tgttgcgggc
     2401 gccgatctgg aggcagtcgg cgtaggtcgc gacggtctcg acgtcgcccg gcgtgaggac
     2461 ctcggtgacg atcgcgagcc ccgtctctgc gcgcgcctcc gcgaggagct cgagaccgtc
     2521 gacgcccagc ccgcggaacg tgtacggcga cgagcgcggc ttgaacgcgc cgccgcgaag
     2581 cacctttgcg cccgcgcgct tcaccgcggc cgcggtcgcg tgcacctgcg cttcgctctc
     2641 cgccgagcac ggtccggcga tgacgatgac ctcgtcaccg ccgatccgca gatcgccaat
     2701 cgtgatgacc gtgtcctcgg gcttgaaggt gcggctcgcc agcttgtagg gctgcgtgat
     2761 gcgcacgact tcatgcacgc ccggcagcag ctcgatcaat cgcggatcga ccttgccgcc
     2821 gccgacgacg ccaagcacga tgcgcgaggc gccggtcgag cg
//
LOCUS       DASTZR010000405         2023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_408979, whole genome shotgun sequence.
ACCESSION   DASTZR010000405 DASTZR010000000
VERSION     DASTZR010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408979"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2006
                     /locus_tag="VFV20_09005"
     CDS             <1..2006
                     /locus_tag="VFV20_09005"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEU5288528.1"
                     /translation="DTINTGIYVIEKRVLERYGIGEAFDFSKDLFPKLLADGEPLYGY
                     VAQGYWTDVGSISEYARANADVLLGRVRTSSLGREILPGVFAAGDVEIDPTAKLTGPI
                     YLGNGVKIGASTEIIGPTVLRDYVSVDYGALVDRSLVWRNTYIGDRTELHGAIVGRQC
                     ALKSRVTLEEGSVIGDHSVVNDGARVRSQVKIWPDKQVESGAVVGSSLIWGSQGRRAL
                     FGRFGVTGLVNIDLTPEFAARLGASYASTLPLGSTVTMNRDQHRSSRMLKRALMGGSV
                     SAGVHVADLSQAPLPIGRFHTRRIGAAGGVHVRVSPFDVRVCDLKFFDRQALDMDKAQ
                     ERKVETAFFREDFRRVPYEDVGAIYESPRVGEAYSEAFLSEIVHRREVADRKFKIVVN
                     YSHGTAAQFLPQLLSALGIEMIAINGVVSENVGSRSFEEFQEQKRELAAIVSALSASA
                     GVIIDAGGEKAFFVDDRGRMTSDMQFLAAFAALSARVTPGVVAVPVFAPSSVDRIVTE
                     AGGRVQRVRASASAQMDIAVREHPLVVADGVGGFIFPRFHPSFDGLFATVRLLELLAV
                     TDKALADVIDETPAANVARLQVPCPWEEKGRVLRVLAQDPRTERVRQIDGVKHQEDGE
                     WVLVLPDADRPLFNVYAEARDEGRAWSLAQEYAERLEQLRAGG"
BASE COUNT          291 a          686 c          723 g          323 t
ORIGIN      
        1 cggacacgat caacaccggc atctacgtga tcgaaaagcg cgtgctcgag cggtacggga
       61 tcggcgaggc gttcgacttc agcaaggatc tcttcccgaa gctgctcgcc gacggagagc
      121 cgctctacgg ctacgtcgcg caggggtact ggacggacgt cggctcgatc tccgagtacg
      181 cccgtgcgaa tgccgacgtg ctcctcggcc gggtgcgcac gagctcgctg ggtcgcgaga
      241 tcctgcccgg cgtgttcgcg gcgggcgacg tcgagatcga tccgaccgcg aagctcacgg
      301 gcccgatcta cctggggaac ggcgtcaaga tcggtgcgtc caccgagatc atcggtccga
      361 ccgtcctgcg cgactacgtc tcggtggact acggcgcgct cgtggaccgt tcgctggtgt
      421 ggcgcaacac gtatatcggt gaccgtaccg agctccacgg cgcgatcgtc ggccggcagt
      481 gcgcgctcaa gtcgcgcgtg acgctcgagg agggcagcgt catcggcgac cactcggtcg
      541 tgaacgacgg cgcgcgggtg cggagccagg tgaagatctg gcccgacaag caggtcgaga
      601 gcggcgcggt ggtgggctcg agcctcatct gggggtcgca gggacgtcgt gcgctcttcg
      661 gccgcttcgg cgtcaccggc ctcgtgaaca tcgacctcac gccggagttc gccgcgcggc
      721 tcggcgcctc gtacgcgtcc accctgcccc tcggcagcac cgtcacgatg aatcgcgacc
      781 agcaccgctc gagccggatg ctcaagcggg cgctgatggg cggcagcgtg tccgccggcg
      841 tgcacgtcgc ggacctgtcg caggcgccgc tgccgatcgg ccgcttccac acgcgacgca
      901 tcggcgccgc cggtggcgtc cacgtgcgag tctcgccgtt cgacgtccgc gtgtgcgatc
      961 tcaagttctt cgaccggcag gcgctcgaca tggacaaggc ccaggagcgg aaggtggaga
     1021 cggcgttctt ccgcgaggac ttccggcgcg tgccctacga ggacgtgggc gcgatctacg
     1081 agtcgccccg ggtcggggag gcgtacagcg aggccttcct ctccgagatc gtgcaccgcc
     1141 gcgaggtggc ggaccgcaag ttcaagatcg tcgtgaacta ttcgcacggc acggccgcgc
     1201 agttcctgcc gcagctgctc tccgcgctcg gcatcgagat gatcgcgatc aacggggtcg
     1261 tctccgagaa cgtgggctcg cgctccttcg aggagttcca ggagcagaag cgcgagctcg
     1321 cggcgatcgt ctcggcgctg tccgcgtccg cgggtgtcat catcgacgcc ggtggagaga
     1381 aggcgttctt cgtcgacgac cgcgggcgca tgacctcgga catgcagttc ctcgcggcgt
     1441 tcgccgcgct gtcggcgcgc gtgacgcccg gggtggtggc tgtcccggtg ttcgcgccgt
     1501 cgtccgtcga tcggatcgtg acggaggccg gcgggcgcgt gcagcgcgtc cgcgcatcgg
     1561 cgagcgccca gatggacatc gccgtccgag agcatccgct cgtcgtggcc gacggcgtcg
     1621 gcggcttcat cttcccgcgc ttccatccct cgttcgacgg cctcttcgcg acggtccgcc
     1681 ttctcgagct gctcgcggtc accgacaagg ccctcgccga cgtcatcgac gagacgcccg
     1741 ccgcgaacgt ggcgcgcctg caggtcccgt gtccgtggga ggagaagggc cgcgtgctgc
     1801 gcgtcctggc gcaggatccg cgcaccgaac gcgtgcgcca gatcgacggc gtcaagcatc
     1861 aggaggacgg tgagtgggtg ctcgtgctcc ccgacgccga ccggccgctc ttcaacgtgt
     1921 acgccgaggc gcgtgacgaa ggacgcgcgt ggagcctcgc gcaggagtat gccgagcgcc
     1981 tcgagcagct ccgggcgggc ggatgacggt ccgcgtcgcg atc
//
LOCUS       DASTZR010000406         4201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_43228, whole genome shotgun sequence.
ACCESSION   DASTZR010000406 DASTZR010000000
VERSION     DASTZR010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43228"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VFV20_09010"
     CDS             complement(<1..239)
                     /locus_tag="VFV20_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288529.1"
                     /translation="MGPLGGTGGTATFPVWSGEGDGDGVGVGVAACVGAAVGACVGAA
                     VGAVVGCAVCCCVADGVADGVAVALGDALALALALA"
     gene            220..546
                     /locus_tag="VFV20_09015"
     CDS             220..546
                     /locus_tag="VFV20_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288530.1"
                     /translation="MPPSGPIGVYGKVTDSATGGPLRGICVTLGQPGAICWAITDING
                     NYTINAASTVQPGSTFQLYFFTCRAGVISLDGSTCANAAPGYATQSTDLFPLSVVVRK
                     DWSVHK"
     gene            complement(566..1753)
                     /locus_tag="VFV20_09020"
     CDS             complement(566..1753)
                     /locus_tag="VFV20_09020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5288531.1"
                     /translation="MSKPKTLVVVQRYGDGVAGGAEAHARQLVNRLKPHLDIEVLTTA
                     SSDYRTWDNTFTAGIDWVDDVPVRRFPVLRPRAWDFKLYERRAFSPQHTLEDERTFVD
                     AQGPYAPDLLEHLWRSGGDADHILFFTYIYYPTVRGLPLVPERAVLVPTAHDEPALGL
                     SIYEPVFHAPRAIAFNTEEERALVHERFRNERVPSDILGVGVDVPADRSADRFRERFA
                     IEGPFVLYIGRIVESKGCRELFAHWQRFRKHDPDRRMTLVLAGQAEMAIPKRPDIVHL
                     GVVSDQEKFDALEAATALAVPEVLSSMSMVTLEAWACAKPVICDTRSPVVWGMARRAG
                     AGLAFGDAAEFGEIVAMLADDDGLAARLGESGRAFVARTYTWPRIVGTYLDLFAEVRA
                     RNA"
     gene            complement(1750..2895)
                     /locus_tag="VFV20_09025"
     CDS             complement(1750..2895)
                     /locus_tag="VFV20_09025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5288532.1"
                     /translation="MNVLVVVQRYGADVTGGSEAHARTAAVRLSLRHTVEVATTTAQD
                     YWTWANHYASGRALEDGIVVHRFPVASGRREDFKDIERHVLMEAHTLADEQGWLAAQG
                     PHCPALLDFLHENGRIYDAVVFYTYIYEPTALGLPIVPERAALISTAHDEGPLGLAPY
                     RALFQMPRAFGFLTPEERDLVQGRFHNEHIPSELIGIGMDPAPDHDDAYRTRFADGPL
                     VAYLGQVSEGKGVDDLLGLWDAYRSDGGRGTLAIAGTVRMQLPRRDDVVVLDRVNDAE
                     KWSLLAAADALVLPSRFESLGIVLLEAWQVGTPVLVPRANAVTSGQVARSGGGVAYDR
                     DTFADALGMLLADGPAMGARGRAWVRDECSWHAFDDRLERLVGLAAS"
     gene            complement(2904..>4201)
                     /locus_tag="VFV20_09030"
     CDS             complement(2904..>4201)
                     /locus_tag="VFV20_09030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5288533.1"
                     /translation="PARVAEALERVVSDGETREDLIARGRGRLEDFSRSAVRGRLVSA
                     LAAGGYDLPDERKKRIVVLSSDQRCGIHHYSLAVCDGLRARGHNVTFVGVRHLDTADL
                     ERKLKYIRDDVETILIEHEAGIFRDVPFVRALLDLRRRGFPIVLSMHELEPEKFHHYR
                     RLSAALHYRPRYGAFLEWLRMPWVALRMSDWFIRYRTVLALMGGFPERLIVHSRRSAR
                     WLELLTRHEEKRDEFPLVVMPLEATDLPRDASEKRALRERLRLPADRFVFVSPGFFFA
                     RKRYIEVIRALPDDAVLVLSGTRSDWEPRYFDEVMEIAKGKANVVVNTDYDTMGDFVA
                     ASDCVVLFYEDVFQSAVVTQAVWAGLPCIFSTAEGFAPYHGAGVVVRDTGELADAMRD
                     VQRPERYAALVRNVRILRRLLSPERNAERYIAGVSTKGR"
BASE COUNT          640 a         1503 c         1494 g          564 t
ORIGIN      
        1 gcgagcgcga gcgcgagcgc gagcgcgtca ccgagcgcga ccgcaacgcc gtccgcgacg
       61 ccatcggcga cgcagcagca gacggcgcag ccgacgaccg cgcccacggc cgcaccgacg
      121 caggcgccga cggccgcgcc cacgcaggcc gccactccga cgccgacgcc gtcaccgtca
      181 ccctcgccgc tccagaccgg gaacgttgcc gtcccaccgg tgccgccgag cggccccatc
      241 ggggtctacg gaaaggtcac cgacagcgcg acgggcggtc cgctgcgcgg aatctgtgtc
      301 acgctcggcc agccgggcgc gatctgctgg gcgatcacgg acatcaacgg caactacacg
      361 atcaacgccg cgtcgacggt ccagcccgga tcgacgttcc agctgtactt cttcacgtgc
      421 cgtgcgggcg tgatctcgct ggatggctcg acctgcgcga acgcggctcc gggctacgcc
      481 acgcagagca ccgatctgtt cccgctctcc gtggtggtgc gcaaggactg gtcggtccac
      541 aagtagtcca acgcgggccg gcgggtcacg cgttgcgggc gcgcacctcg gcgaacaggt
      601 cgaggtaggt cccgacgatc cgcggccagg tgtaggtccg cgcgacgaag gcacggccgc
      661 tctcaccgag ccgcgccgcc agcccgtcgt cgtcggcgag catggcgacg atctcgccga
      721 actccgctgc gtccccgaac gcgaggcccg cgccggcgcg gcgcgccatc ccccacacca
      781 ccggtgagcg cgtgtcgcag atcaccggct tcgcgcaggc ccacgcctcg agcgtgacca
      841 tcgacatgct cgagagcacc tcgggcaccg cgagcgcggt cgcggcctcg agtgcgtcga
      901 acttctcctg gtcgctgacg acgccgagat gcacgatgtc gggccgcttg gggatcgcca
      961 tctccgcctg accggcgagc acgagcgtca tgcgccgatc cgggtcgtgc ttccggaacc
     1021 gctgccagtg cgcgaacagc tcgcggcagc ccttgctctc gacgatccgg ccgatgtaca
     1081 gcacgaacgg tccctcgatc gcgaatcgct cgcggaagcg atccgcggag cgatcggccg
     1141 gtacgtcgac gccgaccccg aggatgtcgc tcggcacgcg ctcgttgcgg aagcgctcgt
     1201 gcaccagcgc gcgctcctcc tcggtgttga aggcgatggc gcgcggcgcg tggaacaccg
     1261 gctcgtagat cgagaggccg agcgcgggct cgtcgtgcgc ggtcggcacg aggaccgcgc
     1321 gttcgggcac gagcggcagt ccgcgcaccg tcgggtagta gatgtaggtg aagaagagga
     1381 tgtgatccgc gtcgccgccg ctccgccaca ggtgctcgag cagatcgggc gcgtacggac
     1441 cctgcgcgtc cacgaacgtg cgctcgtcct cgagcgtgtg ctgcggcgag aacgcgcggc
     1501 gctcgtagag cttgaagtcc cacgcgcgcg ggcgcagcac cgggaagcgg cgcaccggca
     1561 cgtcatcgac ccagtcgatg ccggcggtga aggtgttgtc ccaggtgcgg tagtcggacg
     1621 acgcggtggt gaggacctcg atgtcgagat gcggcttcag ccgattcacg agctggcgcg
     1681 cgtgcgcctc ggccccgccg gcgacgccgt cgccgtagcg ctggaccacc acgagcgtct
     1741 ttggcttgct cacgatgccg ccagtccaac aagccgctcg aggcggtcgt cgaaggcgtg
     1801 ccacgagcac tcgtcgcgca cccacgcgcg gccgcgcgcg cccatcgccg gtccgtccgc
     1861 gaggagcatg cccagcgcgt cggcgaacgt gtcgcggtcg tacgcgacgc cgccgccgga
     1921 gcgcgccacc tgcccgctcg tgacggcgtt cgcgcgcggg acgagcaccg gcgtcccgac
     1981 ctgccacgcc tcgagcagca cgatgccgag gctctcgaac cgcgacggca gcacgagcgc
     2041 gtccgcggcc gcgaggagcg accacttctc ggcgtcgttc acgcggtcca gcacgacgac
     2101 gtcgtcgcgt ctcggcagct gcatccgcac cgtcccggcg atcgcgagcg tcccccgtcc
     2161 gccgtcgctc cggtaggcgt cccagaggcc gagcagatcg tccacgccct tgccctcgct
     2221 cacctggccg agatacgcga cgagcgggcc gtcggcgaac cgcgtccggt acgcgtcgtc
     2281 gtgatcgggc gcaggatcca tgccgatgcc gatgagctcg ctcgggatgt gctcgttgtg
     2341 gaaacggccc tgcacgaggt cgcgctcctc gggcgtcagg aagccgaagg cgcgcggcat
     2401 ctggaagagc gcgcgatatg gcgcgaggcc gagcggcccc tcgtcgtgcg ccgtcgagat
     2461 gagcgccgcg cgctcgggga cgatcggcag gccgagcgcc gtcggctcgt agatgtatgt
     2521 gtagaagacg accgcgtcgt agatgcgtcc gttctcgtgc aggaagtcga gcagcgccgg
     2581 acagtgcggg ccctgcgccg cgagccagcc ctgctcgtcg gcgagcgtgt gcgcctccat
     2641 gaggacgtgc ctctcgatgt ccttgaaatc ctcgcgccgt cccgatgcga ccgggaagcg
     2701 atgcacgacg atgccgtcct cgagcgcgcg acctgaggcg tagtgattcg cccaggtcca
     2761 gtagtcctgc gccgtcgtgg tcgcgacctc gacggtgtgc cggagcgaga gtcgcaccgc
     2821 ggcggtgcgc gcgtgcgcct cggatccgcc ggtgacgtcc gcgccgtagc gctggacgac
     2881 gacgaggacg ttcacgacgg cgctcagcgc cccttcgtgg acacgccggc gatgtaccgc
     2941 tccgcgttgc gctcgggcga gagcaggcgc cgcaggatgc ggacgttccg cacgagcgcg
     3001 gcgtaccgct ccggccgctg cacgtcgcgc atcgcgtcgg cgagctcgcc ggtgtcgcgc
     3061 acgacgacgc cggcgccgtg gtacggcgcg aacccctcgg cggtcgagaa gatgcacggc
     3121 agccccgccc acaccgcctg cgtcacgacg gcgctctgga agacgtcctc gtagaagagg
     3181 acgacgcagt ccgaggccgc gacgaagtcg cccatcgtgt cgtagtcggt gttcacgacg
     3241 acgttcgcct tgcccttcgc gatctccatc acctcgtcga agtagcgcgg ctcccagtcc
     3301 gagcgcgtcc ccgagagcac gagcacggca tcgtccggca gcgcgcggat gacctcgatg
     3361 tagcgcttgc gcgcgaagaa gaagccgggc gagacgaaga cgaacctgtc cgcagggagc
     3421 cggaggcgct cgcgcagcgc gcgcttctcg gacgcgtccc tcggcagatc ggtcgcctcg
     3481 agcggcatga ccacgagcgg gaactcgtcg cgcttctcct cgtgccgggt gagcagctcg
     3541 agccagcgcg ccgagcgccg cgagtgcacg atcaggcgct cggggaagcc gcccatgagc
     3601 gcgagcacgg tgcggtagcg gatgaaccag tccgacatgc gcagcgcgac ccacggcatg
     3661 cgcagccact cgaggaacgc gccgtagcgc gggcggtagt ggagcgcggc ggagaggcgc
     3721 cggtagtgat ggaacttctc gggctcgagc tcgtgcatcg agagcacgat cgggaagccg
     3781 cggcgccgga ggtcgaggag cgcgcgcacg aagggaacgt cgcggaagat cccggcctcg
     3841 tgctcgatga ggatcgtctc cacgtcgtcg cggatgtact tgagcttccg ctcgaggtcc
     3901 gcggtgtcga ggtgacggac gccaacgaac gtcacgttgt gcccgcgggc acgcagcccg
     3961 tcgcagaccg cgagcgagta gtggtggatg ccgcagcgct ggtcgctcga gagcacgacg
     4021 atccgcttct tccgctcgtc gggcaggtcg tatccgcccg cggcgagcgc cgacacgagg
     4081 cgcccgcgca ccgccgaccg agagaagtcc tcgagacgcc cgcggccgcg cgcgatgagg
     4141 tcctcgcgcg tctcgccgtc ggagaccacg cgctcgagcg cctcggccac gcgcgcgggc
     4201 g
//
LOCUS       DASTZR010000407         4392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1067192, whole genome shotgun sequence.
ACCESSION   DASTZR010000407 DASTZR010000000
VERSION     DASTZR010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067192"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VFV20_09035"
     CDS             <1..427
                     /locus_tag="VFV20_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288534.1"
                     /translation="VVSTAVLYIAARDRRYGEDPGWRLWLATVVAASVTAAAFSFLRT
                     TANEEVRITLIGEDPSGVTQEAAAFWLLSSGVAILLTARGAVRGSLGALLMLTGTQLL
                     VQLAPGPQLAFTLLLSWLEIVVALCGAFLILNERAVREA"
     gene            424..1773
                     /locus_tag="VFV20_09040"
     CDS             424..1773
                     /locus_tag="VFV20_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEU5288535.1"
                     /translation="MIVPIVLLGGGALVAFAIRKRVEIVGPVTTALILAAIWWTASSP
                     QESTEVLGLQLRLSPLAQLGSIILLASLLVLVLAVWLDEPAYNFFPTALGAGAAVIAV
                     LTLTTPIAIYAVLLLALLLPVGSFTFQVTRNRSAEAAYRHFSFVALGGAMGIAALALA
                     GSLPREQPPATFVVLVVIVVVAFALKLAAIPFHTHAALLATEVPASALALYFGVLVPT
                     TFIAFAEILTLSGLLPAIVQISKVQDLLLGLGTVSAVGGAFLATGSTELRRVATYSVI
                     ANLGQALVGIATLSGPGIVGAISIALVTGAGATQQLLAAGAIERRSGADRPNATVAPL
                     ASLAFAIGGFAMIGAPPLVAFPGHFFVELIAFSYSGTLGALLVISTLFVLIAQLRAAI
                     SIFRVAPERWRIERRPIAGIAGALVFVALLGAGIQPDSFLRPIAVFADEFIRALRPL"
     gene            complement(1782..2003)
                     /locus_tag="VFV20_09045"
     CDS             complement(1782..2003)
                     /locus_tag="VFV20_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288536.1"
                     /translation="MISRALLRAHQLRIIGPGQRYLMEREWLPDGRVMQRVNEGTPDA
                     THEWKEIGRWRDLARERAAIAEQGWEIDG"
     gene            complement(2000..2479)
                     /gene="ndk"
                     /locus_tag="VFV20_09050"
     CDS             complement(2000..2479)
                     /gene="ndk"
                     /locus_tag="VFV20_09050"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015491647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU5288537.1"
                     /translation="MSPESNVQRTLVILKPDAVQRGIAGEILARFERRGLRIAALRLV
                     KVERSLAEKHYEIHKGKFFYEDLVKMISASPVIAFVLEGPNAIAVVRAMVGATRPHEA
                     APGTIRGDWALVGLRNLVHASDAPETAGTEIELWFPEGVVTYSRDVEKWMTEDKAPS"
     gene            complement(2476..3765)
                     /locus_tag="VFV20_09055"
     CDS             complement(2476..3765)
                     /locus_tag="VFV20_09055"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU5288538.1"
                     /translation="MDHTKRTLPNGVRVLVAEMPEARSASIAVFVGAGSRMEDRTNAG
                     TGHFLEHIVFKGTSKRPTAAEISQEIESRGGAVNAATDKEVTVFWSRLPARHWRVALD
                     VVADLIRAPMLRPGDVDMEKRVVIEELRMYRDQPQDRVHTLIDELLYPRHPLGWEVAG
                     RETVVNALDADRLRDFMTRGYVPSRIVVAVAGRVAADEVFDAVATLFGDITPRVAPRL
                     SPAPKAGRVRMKLLGRRTEQAHVCIGWRGVPQEHPDKYPLDMLNAILGEGMSSRLFLE
                     LREKRALAYDVHSFSANYVDAGHLVIYAGVTPDRISEVIEAALAEVTRLRDHIVPEDE
                     IERVRDFNKGRLELRLEDSRGVSNWLAGQELFLDRVRTVEEVCAIIDGISTQDVQRVA
                     RQYLRPELAYIAAVGPRASIAKVRVPGAEVVDMEIAS"
     gene            complement(3811..4167)
                     /locus_tag="VFV20_09060"
     CDS             complement(3811..4167)
                     /locus_tag="VFV20_09060"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEU5288539.1"
                     /translation="MNASRAAGGLGALALDAGVSATARAHSLAESRVGYVYHDGPDGT
                     AASRNVPACGTGWYGENTGKVWQGTVDRQHIEFMSEPWAPINHRTNIMDPNFRRVGIG
                     AVQGPDALYMTMVFCR"
     gene            4172..>4392
                     /locus_tag="VFV20_09065"
     CDS             4172..>4392
                     /locus_tag="VFV20_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288540.1"
                     /translation="MSIRSSASVVVAVITGATATGAAVGRGVGRGVGGGAGVGSGVGS
                     AVGSGVGSGVAIGVGSGVGDADTYSATDMP"
BASE COUNT          552 a         1582 c         1512 g          746 t
ORIGIN      
        1 cgtggtgtcc acggccgtcc tctatatcgc cgcccgcgac cgccgctacg gcgaggatcc
       61 gggctggcgg ctctggctcg cgaccgtcgt ggcggcctcc gtcaccgcgg cggcgttctc
      121 gttcctgcgg accaccgcga acgaagaggt ccgcatcacg ctgatcggcg aggacccgag
      181 cggcgtcacg caggaggccg ccgcgttctg gctgctctcg tcgggtgtgg cgatcctgct
      241 cacggcacgc ggcgccgtcc gcggatcgct cggtgcgctg ctcatgctca ccgggacgca
      301 gctcctcgtg cagctcgcac ccgggccgca gctcgcgttc acgctcctgc tctcgtggct
      361 ggagatcgtc gtggccctct gcggcgcgtt cctgatcctc aacgagcgtg cggtgcgcga
      421 agcgtgatcg tgccgatcgt gctcctcggc ggcggcgccc tcgtggcgtt cgcgatccgc
      481 aagcgcgtcg agatcgtcgg acccgtcacg acggcgctca tcctcgcggc gatctggtgg
      541 acggcctcgt cgccgcagga atcgaccgag gtgctcggcc tgcagctgcg gctctcgccg
      601 ctcgcgcagc tgggctccat catcctgctc gcgtcgctgt tggtgctcgt gctcgccgtc
      661 tggctcgacg agcccgccta caacttcttc cccaccgcac tcggcgcggg cgcggcggtc
      721 atcgcggtcc tcacgctcac gacgccgatc gcgatctacg ccgtcctgct cctcgcgctg
      781 ctgctgcccg tcggctcctt cacctttcag gtcacacgca accggagcgc cgaggcggcg
      841 tatcggcact tctcgttcgt cgcgctcggt ggtgccatgg gtatcgcggc gctcgcgctc
      901 gcggggagcc tgccgcggga gcagccgccc gcgacgttcg tcgtcctcgt ggtgatcgtc
      961 gtcgtcgcgt tcgcgctgaa gctcgccgcc atcccgttcc acacgcacgc ggcactgctc
     1021 gcgaccgagg tgccggcgtc ggccctcgcg ctgtacttcg gcgtgctcgt gccgacgacc
     1081 ttcatcgcgt tcgccgagat cctcaccctt tcggggctgc tccccgcgat cgtgcagatc
     1141 tcgaaggtgc aggacctgct gctcggcctc ggcacggtgt cggcggtcgg cggtgccttc
     1201 ctcgcgaccg ggtcgaccga gctgcgacgc gtcgccacgt actcggtgat cgccaacctc
     1261 gggcaggcgc tcgtcggcat cgcgacgctc tcgggtcccg gcatcgtcgg cgcgatctcc
     1321 atcgcgctcg tgaccggtgc aggggccacg cagcagctgc tcgccgcggg cgccatcgag
     1381 cgccgctcgg gcgcggaccg tccgaacgcc acggtcgcgc cgctcgcctc gctcgcgttc
     1441 gcgatcggcg gattcgcgat gatcggcgcg ccgccgctcg tcgcgttccc tgggcacttc
     1501 ttcgtcgagc tgatcgcgtt ctcgtactcc ggcacgctcg gcgccctgct ggtgatctcg
     1561 acgctcttcg tcctcatcgc gcagctgcgc gcggcgatct cgatcttccg cgtcgcgccc
     1621 gagcgctggc ggatcgagcg ccggccgatc gccgggatcg ccggggcgct ggtcttcgtc
     1681 gcgctgctcg gcgcggggat ccagccggac agcttcctgc ggccgatcgc ggtcttcgcc
     1741 gacgagttca tccgtgcgct ccgcccgctc tgaccagggt cttagccgtc gatctcccag
     1801 ccctgctcgg cgatcgcggc gcgctccctc gcgagatcgc gccagcggcc gatctccttc
     1861 cattcgtgcg tcgcatcggg tgtgccctcg ttgacccgct gcatgacgcg gccgtcgggc
     1921 agccactcgc gctccatcag gtagcgctga cccggtccga tgatccgcag ctgatgcgcg
     1981 cggagcaggg cgcgcgagat cacgacgggg ccttgtcctc ggtcatccac ttctcgacgt
     2041 cgcggctgta cgtgacgacg ccctcgggga accacagttc gatctcggtg cccgccgtct
     2101 ccggcgcatc actggcgtga acgaggttgc gcagcccgac gagcgcccag tcgccgcgga
     2161 tcgtgccggg cgccgcctcg tgcgggcgcg tggcgccgac catcgcgcgc acgacggcga
     2221 tcgcgttcgg cccttcgagg acgaacgcga tcacgggcga ggcactgatc atcttcacca
     2281 ggtcttcgta gaagaacttg cccttgtgga tctcgtagtg cttctcggcg agcgaacgct
     2341 cgaccttcac gagacggagc gcggcgatgc gcaggccgcg gcgttcgaag cgcgcgagga
     2401 tctcgcccgc gatgccgcgc tggacggcgt cgggcttgag gatcacgagt gtgcgctgga
     2461 cgttcgattc ggggctcacg atgcgatctc catatccacc acctccgcgc ctgggacacg
     2521 aaccttggcg atcgaggcgc ggggcccgac cgccgcgatg taggcaagct cgggtcgcag
     2581 gtactgccgg gcgacgcgct gcacgtcctg cgtgctgatg ccgtcgatga tggcgcagac
     2641 ctcctcgacc gtgcgaacgc ggtcgaggaa gagctcctgc ccggcgagcc agttcgagac
     2701 gccacggctg tcctcgaggc gcagctcgag ccgtcctttg ttgaagtcac gcacgcgctc
     2761 gatctcgtcc tcggggacga tgtgatcgcg aagacgcgtg acctccgcga gcgcggcttc
     2821 gatgacctcg ctgatgcgat ccggcgtcac gcccgcgtag atcacgaggt gacccgcgtc
     2881 cacgtagttc gcgctgaagg agtggacgtc gtacgcgagg gcgcgcttct cgcggagctc
     2941 gaggaagagc cggctcgaca taccctcgcc gaggatcgcg ttgagcatgt ccagcgggta
     3001 cttgtcggga tgctcctgcg gcacaccgcg ccatccgatg cacacatgcg cctgctccgt
     3061 gcggcgtccg aggagcttca tgcgcacgcg tccggccttc ggcgcagggc tgagcctcgg
     3121 cgccacgcgc ggggtgatgt cgccgaacaa ggtggcgacg gcatcgaaga cctcgtccgc
     3181 ggcgacgcga ccggccaccg cgacgacgat gcgcgagggc acgtatccac gcgtcatgaa
     3241 gtcgcgcagg cggtccgcgt cgagggcatt gacgacggtc tcgcggccgg cgacctccca
     3301 gccgagcgga tgacgcggat agagcagctc gtcgatgagc gtgtgcacgc ggtcctgcgg
     3361 ctgatcgcgg tacatgcgga gctcttcgat gacgacgcgc ttctccatgt cgacatcgcc
     3421 cggccgcagc atcggcgcgc ggatgaggtc ggcgacgacg tcgagggcga cccgccagtg
     3481 ccgcgccgga agtcgcgacc agaagaccgt gacctccttg tcggtcgccg cgttcacggc
     3541 cccgccgcgg ctctcgatct cctgactgat ctcggcggcg gtggggcgct tggacgtccc
     3601 cttgaacacg atgtgctcga ggaagtgccc ggtgcccgcg ttcgtgcggt cctccatgcg
     3661 cgagcccgcg ccgacgaaga ccgcgatcga tgcggaccgc gcttcgggca tctcggcgac
     3721 gagcacgcgc acgccatttg gaagggtgcg cttcgtgtga tccaaggtga ggaacgctac
     3781 cagaggcagg gctgagacag cgtcacgcgg tcagcggcag aacaccatcg tcatgtagag
     3841 cgcgtccggc ccctgcaccg caccgatgcc cacgcgccgg aagttcggat ccatgatgtt
     3901 ggtgcggtga ttgatcgggg cccacggctc cgacatgaat tcgatgtgct ggcgatcgac
     3961 ggtcccctgc cacaccttgc cggtgttctc gccgtaccag cccgtcccgc atgcgggcac
     4021 gttgcgcgag gccgcggtgc cgtcgggccc gtcgtgatac acgtagccga ccctcgactc
     4081 cgcaaggctg tgtgcgcgcg cggtcgcgga cacgcccgcg tccagcgcga gcgccccgag
     4141 gccgcccgcc gcgcgcgagg cgttcatcag cgtgagcatc cgcagctccg cctccgtggt
     4201 cgtggcggtg atcaccggcg cgaccgcgac cggggcggcc gtcgggcgcg gcgtcggtcg
     4261 cggcgtcggc ggcggtgccg gcgtcggctc gggcgtcggc tccgccgtgg gttcgggcgt
     4321 cggctccggc gttgccatcg gcgtcggctc gggcgtcggc gacgccgaca cgtactcggc
     4381 cacggacatg cc
//
LOCUS       DASTZR010000408         2531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_518797, whole genome shotgun sequence.
ACCESSION   DASTZR010000408 DASTZR010000000
VERSION     DASTZR010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518797"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /gene="tyrS"
                     /locus_tag="VFV20_09070"
     CDS             complement(<1..1025)
                     /gene="tyrS"
                     /locus_tag="VFV20_09070"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU5288541.1"
                     /translation="MPSSPTNAELDEFLTRAVAEVIVREEFATLLRSGERQLRIKQGF
                     DPTRPNLHIGHAVGLRKLRTLASWGHEIVIIIGDWTTQIGDPTDKDETRPTMSHEQVL
                     KNADTYLAQFHKFVPKENTRIVFQSEWYGKFGLRDVIDLASKFTAQQMLQREEFRKRM
                     EKKTPIPIKDLLYPLLQAYDSVAIEADVEMGGTDQLFNILAGRELQTQLGQRAQQVFI
                     VPLLEGLDGEKMSKSKPKTGIWLTDPANEVYGKVMSIDDALMPHYFEWATDLPLEEVR
                     SIVGALGRKELGPKEAKERLAWQITSELHSPAAADEAKAAFARQFQKGQAPEDMRSID
                     LTADGER"
     gene            1269..>2531
                     /locus_tag="VFV20_09075"
     CDS             1269..>2531
                     /locus_tag="VFV20_09075"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEU5288542.1"
                     /translation="MGAAAAFAFFSADLPAPEELAKDPLALSTKVYDRTGTTILYQFE
                     VERREIVGIDQVPQQMIDATLAAEDKTFWTNPGVDIFGILRAARDDLLHKTVVSGAST
                     ITQQLVKQRIVGDEVSITRKIKEAILSIEVTRTYSKKQILELYLNQIYYGNQAYGIKA
                     AAETYFGKSDLSQLTLAESALLAGLPQQPSALDPSDPENVASAKERRAYVLGEMVDMG
                     AITEEQAAAADVEPIKVAGAQITTITAPHFVFQVRDQLKQILGGNESAVTRGGFKVIT
                     TLDMTKQKAAEQNVKDHVEELHNKNVWNAAMVSIDPRNGEVLAYVGSVDYYNREDPRV
                     QGQFDVAGIGQRQPGSSFKMFNYLTALKKGATPATVVVDARTDFTGRADPSNMNRSTC
                     GYCPENADLQYHGPVTMRQALRESRNVPA"
BASE COUNT          425 a          856 c          830 g          420 t
ORIGIN      
        1 tcgcgctccc cgtcggccgt gaggtcgatg gaccgcatgt cctcgggcgc ctggcccttc
       61 tggaactgcc gcgcgaacgc ggccttcgcc tcgtcggcgg cggcgggcga gtgcagctcg
      121 ctcgtgatct gccacgcgag ccgctccttt gcctccttcg gccccagctc cttgcggccg
      181 agcgcaccga cgatgctgcg cacctcttcc agcgggaggt ccgtcgccca ttcgaagtag
      241 tgcggcatca acgcgtcgtc gatcgacatg accttgccgt acacctcgtt cgccggatcg
      301 gtgagccaga tgccggtctt cggcttcgac ttgctcatct tctcgccgtc gaggccctcg
      361 aggagcggca cgatgaagac ctgctgcgcg cgctggccga gctgcgtctg cagctcgcgg
      421 ccggcgagga tgttgaagag ctgatcggtc ccgcccatct cgacgtcggc ctcgatggcg
      481 accgagtcgt atgcctggag cagcgggtag aggaggtcct tgatggggat gggcgtcttc
      541 ttctccatcc gcttgcggaa ctcctcgcgc tggagcatct gctgcgcggt gaatttcgag
      601 gcgaggtcga tcacgtcgcg caggccgaac ttcccgtacc actcggactg gaagacgatc
      661 cgggtgttct ccttcggcac gaacttgtgg aactgcgcga ggtaggtgtc ggcgttcttc
      721 agcacctgct cgtgggacat cgtcgggcgc gtctcgtcct tgtcggtggg gtcgccgatc
      781 tgcgtcgtcc agtcgccgat gatgatcacg atctcgtggc cccagctcgc gagcgtccgc
      841 agcttgcgga ggccgacggc gtggccgatg tgcaggttcg gccgtgtcgg gtcgaagccc
      901 tgcttgatac ggagctgcct ctctcccgac cgtaacaggg tggcgaattc ctcccgcacg
      961 atcacctcgg cgaccgcgcg cgtcaggaac tcgtcgagct ccgcgttcgt ggggctgctg
     1021 ggcatgcgac agagtttacg agcgaagagc cggtgtgccg cgacgagcgc gcggaaggga
     1081 taggttttcg cggatcgtcc ggttagcatt gatgcatcgc gcgcaacaag aggactcgag
     1141 agcacgcact gaagcgcggg tcataccgcc agctgcgtcg ccggaacggc ggtggcgttc
     1201 gcgcccgcct cgcggtcctt ccctccattc tcgcggccat cctgatcgcc ggcatcgcgc
     1261 tggcgtcggt gggggcggcg gcggcgttcg ccttcttcag tgccgacctg cccgcgcccg
     1321 aggagctcgc gaaggacccg ctcgccctgt ccacgaaggt ctacgaccgc accggaacga
     1381 cgatcctcta ccagttcgag gtcgagcgcc gcgagatcgt cggcatcgac caggtcccgc
     1441 agcagatgat cgacgccacc ctggcggccg aggacaagac gttctggacg aacccgggtg
     1501 tggacatctt cggcatcctg cgcgcggccc gcgacgacct cctgcacaag acggtcgtga
     1561 gcggcgcgtc cacgatcacg cagcagctcg tgaagcagcg catcgtcggc gacgaggtca
     1621 gtatcacccg caagatcaag gaagcgatcc tctcgatcga ggtgacgcgc acctactcga
     1681 agaagcagat cctcgagctc tacctcaacc agatctacta cgggaaccag gcatacggca
     1741 tcaaggctgc cgcggagacc tacttcggga agtcggacct ctcacagctc acgctcgctg
     1801 agtccgcgct cctcgccggc ctgccccagc agccgtccgc actcgacccg tcggacccgg
     1861 agaacgtcgc gagcgcgaag gagcggcgcg cgtacgtcct cggcgagatg gtcgacatgg
     1921 gcgcgatcac cgaggagcag gccgcggcgg cggacgtgga gcccatcaag gtcgccggcg
     1981 cgcagatcac cacgatcacc gcgccgcact tcgtcttcca ggtccgcgat cagctgaagc
     2041 agatcctcgg cggtaacgaa tcggccgtga cccgcggcgg cttcaaggtg atcaccacgc
     2101 tcgacatgac caagcagaag gccgccgagc agaacgtgaa ggaccacgtc gaggagctcc
     2161 acaacaagaa cgtgtggaac gcggccatgg tcagcatcga tccgcgcaac ggcgaggtgc
     2221 tcgcgtacgt cggctcggtc gactactaca accgcgagga tccgcgcgtg cagggccagt
     2281 tcgacgtcgc cggtatcggc cagcggcagc ccggctcctc cttcaagatg ttcaactacc
     2341 tcaccgcgtt gaagaagggc gccacgcccg cgaccgtggt ggtcgacgcg cggacagact
     2401 tcaccggcag ggccgacccc agcaacatga accggagcac gtgtggctac tgtccggaga
     2461 acgcggacct ccagtaccac gggccggtga cgatgcgcca ggcgctgcgc gagtcgcgga
     2521 acgtgcccgc c
//
LOCUS       DASTZR010000409         4185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_179060, whole genome shotgun sequence.
ACCESSION   DASTZR010000409 DASTZR010000000
VERSION     DASTZR010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179060"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VFV20_09080"
     CDS             complement(<1..533)
                     /locus_tag="VFV20_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEU5288543.1"
                     /translation="MILEEGRVVANTKIGELGALIAVHAPGIAQRANAGQFVHVRPGH
                     GWDPLLRRPYSFCRIDRRSGVLELIVKPLGTGGEWIAERHEGDGLDLLGPLGTAFVVR
                     RDTGNLLLVAGGTGIAPMRVIAEREAARRNVTLVMGGRSVSHLWPSERLPAGVEYLTT
                     TEDGSFGTRGRVTDVMP"
     gene            complement(530..964)
                     /locus_tag="VFV20_09085"
     CDS             complement(530..964)
                     /locus_tag="VFV20_09085"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5288544.1"
                     /translation="MTKKRTGEPWKTAAAYGRELTGLSLNLLVRDIAKSLPFYTEVLG
                     FHVHYSDVDFAALERDGVAIQLHADHCYDGLPWAPRLAEAGKRGLGAEIRIMGISPDG
                     AERSAAAHGGAVLVATQDSAHGWRDCILEDPDGYTFAVGLAL"
     gene            complement(961..1572)
                     /locus_tag="VFV20_09090"
     CDS             complement(961..1572)
                     /locus_tag="VFV20_09090"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEU5288545.1"
                     /translation="MTRLFHDGNRELQQRFDTTRIADRIEQVLVGDTISDHDKAFIES
                     RDMFFLATADDSGLPNCSYKGGEPGFVRVVDERTLAFPNYDGNGMYLSMGNVLVNPAV
                     GMLFIDFERGHRMRVNGTASVDAQDPLMAEYPEAQFIVRVRAREVFPNCPRYIHRYRL
                     VERSRFVPKQNHATPVPAWKCAEWSADALPANDPARDPHREVL"
     gene            complement(1599..>4185)
                     /gene="carB"
                     /locus_tag="VFV20_09095"
     CDS             complement(1599..>4185)
                     /gene="carB"
                     /locus_tag="VFV20_09095"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008482005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU5288546.1"
                     /translation="MENLDPMGVHTGDSIVVAPSQTLTDKEYQMLRSAALKIIRALGI
                     EGGCNVQFALHPDRSPSDDPESQTPYHVIEVNPRVSRSSALASKATGYPIARVAAKIA
                     IGKRLDEIPNAVTKKTSAAFEPALDYVVTKIPRWPFDKFAAADRSLGTQMKATGEVMA
                     IDRTFEASLQKALRSLEVKGQGLLWESPGWVEVTDPKAFTERFLGSTATDDRLWRIFA
                     ALRRGASVDDVHERTRVDRWFLRKMSGIVRFAEDELQGKTPTPDLLRRAKRLGFADSD
                     VATLTGMLAADVRRLRAQWGIRPVYKMVDTCAAEFEAVTPYFYSTFEQENEAEPLLGE
                     KAIIIGSGPIRIGQGIEFDCCCVQAAMSLRERGVAPIMVNSNPETVSTDFDASARLYF
                     DPLDEESIASVLDNEGAGDDVAGQVLTQFGGQTALNIADRIAAIGGRIAGTSADAIAL
                     AEDRRRFNDFANALGIPQPRGGTAASISEALAIANEVGYPVLVRPSYVLGGRGMEIVY
                     APEDLERYFHAALEAGTGRVLVDKYLRGKEVEVDAVSDGTDTLVAGIMEHIERAGVHS
                     GDSYAVYPAQNLLPEERERIVELTALIARSLPVKGLLNIQFIVEEGRVWVLEVNPRSS
                     RTVPFLTKVTGVPLVSLAVAISLGSTLAAEGYVRADGIWPDQPLVAVKAPVFSMAKLL
                     DVDTYLGPEMKSTGEVMGVDRSFAPALWKSLVAAGLAPMPSGEVLVTVADKDKDEVVP
                     IIEGFHWLGYDLVATRGTAALIRSLGIDVREVGKLSEGKDEILRVIRSGKCAAVVNTP
                     TLGKTVDRDGFLIRRAAVEARVPCLTSLDTALAVVTALRASAVSYDVAPLIDYRGKAA
                     ATAAD"
BASE COUNT          622 a         1454 c         1467 g          642 t
ORIGIN      
        1 tccggcatga cgtcggtcac ccggccacgg gtcccgaacg agccgtcctc ggtcgtcgtc
       61 aggtactcca cgcccgcggg caggcgctcc gacggccaga ggtgcgagac gctgcggccg
      121 cccatcacga gggtcacgtt ccggcgcgcg gcctcccgct cggcgatgac acgcatgggc
      181 gcgatgccgg tcccgccggc gacgagcagc aggttccccg tgtcgcgcct gacgacgaac
      241 gccgtaccga gcggaccgag gagatcgagc ccgtcaccct cgtggcgttc cgcgatccac
      301 tcgccgccgg tgccgagcgg cttcacgatg agctcgagga cccccgaccg ccggtcgatg
      361 cggcagaagg agtacggccg ccgcagcagc ggatcccagc catggcccgg ccgcacgtgc
      421 acgaactgac cggcgttcgc gcgctgggcg atgccgggcg cgtgcacggc gatgagcgcg
      481 ccgagctcgc cgatcttcgt gttggcgacg acgcggcctt cctcgaggat cacagcgcga
      541 gtcccacggc gaacgtgtag ccgtccggat cctcgaggat gcagtcgcgc catccgtgcg
      601 cgctgtcctg cgtcgcgacg agcacggcac cgccgtgggc cgcggcactc cgctcggcgc
      661 cgtcgggcga gatccccatg atgcggatct cggcgccgag gccgcgcttc cccgcctccg
      721 cgagccgggg cgcccagggc aacccgtcgt agcagtggtc cgcgtgcagc tggatggcga
      781 cgccgtcgcg ctcgagggcg gcgaagtcga cgtcggaata gtggacgtgg aagccgagca
      841 cctcggtgta gaagggcagg ctcttcgcga tgtcacgcac gagcagattg agcgacagtc
      901 ccgtgagctc gcggccatac gccgccgccg tcttccacgg ctcaccggtg cgcttcttgg
      961 tcacaggacc tcgcgatgcg gatcacgcgc cggatcgttc gccggcaacg cgtccgcgga
     1021 ccactcggcg cacttccacg cgggcaccgg cgtcgcatgg ttttgcttcg ggacgaagcg
     1081 cgatcgctcg accagacggt accgatggat gtagcgcggg cagttcggga acacctcgcg
     1141 cgcgcgcacc cggacgatga actgcgcctc cgggtactcc gccatcagcg ggtcctgcgc
     1201 gtcgacgctc gccgtgccgt tcacgcgcat gcggtgtccg cgctcgaagt cgatgaacag
     1261 catcccgacg gcggggttga cgagaacgtt gcccatcgag aggtacatgc cgttgccgtc
     1321 gtagttcggg aacgcgagcg tgcgctcgtc gacgacgcgg acgaaacccg gctcgccacc
     1381 cttgtacgag cagttgggca gcccggaatc gtcggccgtc gcgaggaaga acatgtcgcg
     1441 cgactcgatg aaggccttgt cgtggtcgct gatcgtgtcg ccgacgagga cctgctcgat
     1501 gcgatcggcg atacgcgtgg tgtcgaaccg ctgctgcagc tcgcgattcc cgtcgtgaaa
     1561 gagccgtgtc atgctcgtcc cgtcctccct ggcggccgct agtctgccgc ggtcgcggcc
     1621 gccttccctc gatagtcgat gagcggagcg acatcgtagg agaccgccga tgcgcgcagc
     1681 gcggtgacca ccgcgagcgc cgtgtcgagc gaagtgaggc acggcacgcg agcttccacg
     1741 gcggcccggc ggatgaggaa gccgtcgcgg tcgacggtct tcccgagcgt gggcgtgttc
     1801 acgacggcgg cgcatttgcc ggagcggatg acgcggagga tctcgtcttt gccctcggag
     1861 agctttccca cctcgcgcac gtcgatgccg agggaacgga tgagcgcggc cgtgccgcgg
     1921 gtcgccacga ggtcgtagcc gagccaatgg aagccctcga tgatcgggac gacctcgtcc
     1981 ttgtccttgt cggccaccgt gaccagcacc tcgccgctcg gcatcggcgc gaggccggcc
     2041 gcgaccaggc tcttccatag ggccggcgcg aacgaccgat cgacacccat cacctcgccg
     2101 gtgctcttca tctccggacc gaggtaggtg tccacgtcga ggagcttcgc catggagaac
     2161 accggtgcct tcaccgcgac gagcggctgg tccggccaga tgccgtccgc gcggacgtac
     2221 ccctcggcgg cgagcgtcga gccgagcgag atcgcgaccg cgagggacac cagcgggacg
     2281 ccggtcacct tggtgaggaa cgggacggtg cgcgagctgc gcgggttcac ctcgagcacc
     2341 cacacgcgcc cctcctcgac gatgaactgg atgttgagca gccccttcac cgggagggag
     2401 cgcgcgatca gcgcggtgag ctcgacgatg cgctcgcgct cctccggcag caggttctgc
     2461 gccggataca ccgcgtagga gtcgccggag tgcacgcccg cgcgctcgat gtgctccatg
     2521 atccccgcga cgagcgtgtc ggtgccgtcg gagacggcgt ccacctcgac ctccttgccg
     2581 cggaggtact tgtcgacgag cacgcggccg gtgccggcct cgagggcggc gtggaagtag
     2641 cgctcgaggt cctccggtgc gtagacgatc tccatgccgc ggccgccgag gacgtacgag
     2701 ggacggacga gcaccgggta cccgacctcg ttcgcgatcg cgagcgcctc cgagatcgac
     2761 gccgctgtgc cgccccgcgg ctgcgggatg ccgagcgcgt tcgcgaagtc gttgaagcgt
     2821 cgccgatcct ccgccagggc gatcgcgtcc gcggacgtgc cggcgatgcg gccgccgatc
     2881 gcggcgatgc ggtcggcgat gttgagcgcg gtctggccgc cgaactgcgt gagcacctgg
     2941 ccggccacgt cgtcgcccgc gccttcgttg tcgagcaccg aggcgatgga ctcctcgtcg
     3001 aggggatcga agtagaggcg cgccgacgcg tcgaagtcgg tggacacggt ctcgggattg
     3061 ctgttcacca tgatcggcgc gacgccgcgc tcccgcagcg acatcgcggc ctgcacgcag
     3121 cagcagtcga actcgatccc ctggccgatg cggatcggac ccgagccgat gatgatggcc
     3181 ttctcgccga ggagcggctc ggcctcgttc tcctgttcga aggtcgagta gaagtacggc
     3241 gtgaccgcct cgaactccgc ggcgcaggtg tcgaccatct tgtacaccgg gcggatgccc
     3301 cactgcgcgc gcaggcggcg gacgtccgcc gccagcatgc cggtgagcgt cgcgacgtcc
     3361 gagtccgcga acccgagacg cttggcgcgg cgtagcaggt ccggcgtggg cgtcttgccc
     3421 tgcagctcgt cctcggcgaa gcgcacgatc ccggacatct ttcgcaggaa ccagcgatcg
     3481 acgcgcgtcc gctcgtgcac gtcgtcgacg ctcgcgccgc ggcgcagcgc ggcgaagatg
     3541 cgccagagcc ggtcgtccgt ggcggtgctg cccaggaacc gctcggtgaa ggccttcggg
     3601 tccgtgacct cgacccaccc gggcgactcc cacagcagcc cctgcccctt gacctcgagc
     3661 gagcgcaggg ccttctggag cgatgcttcg aaggtccggt cgatggccat gacctcgccc
     3721 gtcgccttca tctgcgtgcc cagcgaccgg tcggcggccg cgaacttgtc gaacggccag
     3781 cgcgggatct tcgtcacgac gtagtcgagc gccggctcga atgcggcgga ggtcttcttc
     3841 gtgaccgcgt tcgggatctc gtcgaggcgc ttcccgatcg cgatcttcgc agccacgcgc
     3901 gcgatcggat agcccgtcgc cttcgaggcg agcgccgagg agcgcgacac gcgcggattc
     3961 acctcgatga cgtggtacgg cgtctgcgac tcggggtcgt cgctcggaga gcgatccggg
     4021 tgcagagcga actggacgtt gcatccgccc tcgatgccga gcgcgcggat gatcttgagc
     4081 gccgcgctcc gcagcatctg gtactccttg tcggtcagcg tctgcgaagg ggcgacgacg
     4141 atcgagtcac ccgtgtgcac gcccatgggg tcgaggttct ccatg
//
LOCUS       DASTZR010000410         3645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_100089, whole genome shotgun sequence.
ACCESSION   DASTZR010000410 DASTZR010000000
VERSION     DASTZR010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100089"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VFV20_09100"
     CDS             complement(<1..498)
                     /locus_tag="VFV20_09100"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEU5288547.1"
                     /translation="MPLQGKRLAFIGGGTMAEAMMRGLFDKHLVPPSHVLVTGPRRER
                     RAELTKRFGVKALASNAEAARGAHVVVLSVKPQVLPHVLRDLRGELTAEQLVISIVAG
                     APLAVMRDGLDHAAIVRAMPNTPAQIGMGVTAWTATSEVDREQRDRAKAILGALGEEI
                     EVEEES"
     gene            complement(498..1271)
                     /locus_tag="VFV20_09105"
     CDS             complement(498..1271)
                     /locus_tag="VFV20_09105"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEU5288548.1"
                     /translation="MSIADLPELRSPPAATAAQMAEADRVAASELGIPLEVLMENAAR
                     QIAAATRSFRGSVDGRRVVAVCGTGNNGGDALGALRHLHGWGAQVEAVVAAPRDRLRP
                     LAALQLDILDRLGVPCDDAGAMDDAAIVDRLRAADVLLDGLLGYSASGAPRGEIARLI
                     ALMTGGRREGRIVAVDLPSGLDPDTGATLNGGPAGAVRAALTVTLALPKTGLLRAEAR
                     EWVGDLVLADIGIPAKAYEGLGIAAQRLFADGDLVRIIP"
     gene            complement(1268..2023)
                     /locus_tag="VFV20_09110"
     CDS             complement(1268..2023)
                     /locus_tag="VFV20_09110"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5288549.1"
                     /translation="MSDRLEKTRRQWTDAAAAYLASPTHASGEDLDTMMRFADPKSDD
                     VALDLGCGVGHALRRVAAKARLAVGADATSGMLEGARTLLARDGLTNAVLVVTTAEEL
                     PFLDASFDVVVSRLAAHHFFDAGAAFREVHRVLRPGGRFVLSDNYAPDDEALDRFINT
                     LEATRDGTHVRSHTIAGWRELIEQAGFDVTDESRGTIHIETEPWLERARTPAPEAARV
                     RDMLRTAAGPTVETFRIDAMGFDLLKVVLRAVP"
     gene            2174..3007
                     /locus_tag="VFV20_09115"
     CDS             2174..3007
                     /locus_tag="VFV20_09115"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5288550.1"
                     /translation="MRLGRFDVQIFSDGIFRLDGGAMFGVVPKVLWEKQKPADDQNRV
                     AMDMNCLLIRDGANVVLVETGAGPKLNDKQKRNFGVDGPPRLLDELARRGVRPDEVTL
                     VINTHLHFDHAGGNTYRDGERVVATFPRASYVFQRLEWADANAPNERTRGSYLSEDFA
                     PLEAAGRLELIDEAVEILPGIRLDRIQGHTRGTQTVRVTDGKQTLFFSSDFMPDRHHL
                     PLPWIPSQDLYPLETLAAKKVILPRAIAERWIVAFTHDVPRFGHITEQEGRYRFDELA
                     E"
     gene            3030..>3645
                     /locus_tag="VFV20_09120"
     CDS             3030..>3645
                     /locus_tag="VFV20_09120"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5288551.1"
                     /translation="MSERASTLIVGGGIVGAAVAYFLAERRETDVVLLERGELGSGTT
                     AGGLGGIRHQFVDELDVRLSMLAAKTWRVFDELTGAHHDFRQRGYLFLANTDAGLAEL
                     RAAMPLYERVGVNVEMVDPVTIADLVPGMRVDDLRGGRLGAEDGYGDPLQALAGFAAF
                     AQLGGVRLREHVTVRDLLRTGDRVTGVRTDAGDITADRVLLATGC"
BASE COUNT          501 a         1343 c         1277 g          524 t
ORIGIN      
        1 gctttcctcc tcgacctcga tctcttcgcc gagcgcgccg aggatcgcct tcgcgcggtc
       61 gcgctgctcg cggtcgacct cgctggtggc cgtccacgcg gtgacgccca tgccgatctg
      121 cgcgggagtg ttgggcatcg cacgcacgat ggccgcgtgg tcgagcccgt cgcgcatcac
      181 cgcgagcggc gcgccggcga cgatggagat gacgagctgt tccgctgtca gctcgccgcg
      241 caggtcgcgc agcacgtggg gaaggacctg cggcttcacg gatagcacca ccacgtgcgc
      301 gccccgcgcc gcctcggcgt tcgacgcgag cgccttcacg ccgaagcgct tcgtgagctc
      361 ggcacgccgt tcgcgccgcg gaccggtgac gaggacgtgc gagggaggca cgaggtgctt
      421 gtcgaagagg ccgcgcatca tcgcctcggc catcgtcccg ccgccgatga aggcaaggcg
      481 ctttccctgc agtggcatca ggggattatc cgcaccaggt cgccgtccgc gaagagccgc
      541 tgtgcggcga tgccgagccc ctcgtaggcc ttcgcgggga tgccgatgtc ggcgagcacg
      601 aggtcgccga cccactcgcg cgcctcggct cgcagcaagc ccgtcttcgg cagcgccagc
      661 gtgaccgtca gcgccgcgcg gaccgcgccg gcgggcccgc cgttcagcgt ggcgccggtg
      721 tcgggatcca ggcccgaagg cagatcgacc gcgacgatcc ggccctcccg cctgccgccg
      781 gtcatcagtg cgatcaggcg cgcgatctcg ccacgcgggg caccgctcgc gctgtatccc
      841 aggagcccgt cgaggagcac gtccgccgcc cgcaggcgat ccacgatcgc cgcgtcgtcc
      901 atcgcgcccg cgtcatcgca cggcacgccg aggcggtcca ggatgtcgag ctggagcgcg
      961 gcgaggggcc ggaggcggtc gcgcggcgcg gcgacgacgg cctcgacctg cgcgccccag
     1021 ccgtggagat ggcgcagcgc tccgagcgcg tcgccgccgt tgttgcccgt gccgcagacc
     1081 gcgacgacgc gccgcccgtc gacgctccca cggaacgacc gcgtcgccgc ggcgatctgg
     1141 cgggccgcgt tctccatgag gacctcgagc gggatgccga gctcgctcgc cgcgacgcga
     1201 tccgcctcgg ccatctgcgc ggccgtcgcg gcaggcgggc ttcgcagctc cggaaggtcc
     1261 gcgatgctca cggcaccgcg cggaggacga ccttcagcag gtcgaagccc atcgcgtcga
     1321 tccggaacgt ctcgacggtc ggcccggccg ccgtccggag catgtcccgg acgcgcgcgg
     1381 cctccggcgc gggcgtccgc gcgcgctcga gccacggctc cgtctcgatg tggatcgtgc
     1441 cgcgtgactc gtcggtgacg tcgaagccgg cctgctcgat cagctcgcgc catcccgcga
     1501 tggtgtgcga gcgcacgtgc gtgccatcgc gcgtcgcctc gagcgtgttg atgaaccggt
     1561 ccagggcctc gtcatcgggt gcgtagttgt ccgacaggac gaagcggccg cccggacgga
     1621 gcacgcggtg cacctcgcgg aacgcggcgc ccgcgtcgaa aaagtgatgt gcggcgaggc
     1681 gcgagacgac cacgtcgaag gacgcgtcga ggaagggcag ctcctcggct gtcgtcacga
     1741 cgagcaccgc gttggtcagg ccgtcacgcg ccagcagggt gcgggcgccc tcgagcatcc
     1801 cgctcgtcgc gtccgcgccc accgcgagac gcgccttcgc ggcgacgcgg cgcagcgcgt
     1861 ggccgacgcc acagccgagg tccagcgcga cgtcgtcgct cttcggatcc gcgaaccgca
     1921 tcatcgtgtc gaggtcctcg ccgctcgcgt gcgtggggct cgcgaggtag gcggccgccg
     1981 cgtcggtcca ctgccgtcgt gtcttctcga gccgatcgct catagcgtct ttccgaactc
     2041 caccgcgttc tcgcgccggt atcccgccgc ggtccagaag tccatcgcgc tcacgtcgtc
     2101 cttccacacc cgccgcgccg gccgtcgccg tccgcgaact cacggatccg catggctctc
     2161 catactgacg tgcatgcgtc tcggccgctt cgacgtgcag atcttcagcg acggcatctt
     2221 ccgcctcgat ggcggtgcga tgttcggcgt cgtgccgaag gtcctgtggg agaagcagaa
     2281 gcccgccgac gaccagaatc gcgtggccat ggacatgaac tgcctgctca tccgcgacgg
     2341 cgcgaacgtc gtgctcgtcg agaccggtgc gggtccgaag ctgaacgaca agcagaagcg
     2401 caacttcggg gtcgacggac cgccgcgact gctcgacgag ctggcgcgcc ggggcgtgcg
     2461 gcccgacgag gtgacgctcg tcatcaacac gcacctgcac ttcgaccacg cgggcggcaa
     2521 cacctaccgc gacggcgagc gcgtcgtcgc gacgttcccg cgcgcgtcct acgtgttcca
     2581 acggctcgag tgggccgacg cgaacgctcc gaacgagcgc acccgcggct cctacctgtc
     2641 ggaggacttc gcgcccctcg aggccgccgg caggctcgag ctcatcgacg aggcggtgga
     2701 gatcctgccg ggcatccggc tcgaccgcat ccagggccac acgcgcggca cgcagacggt
     2761 acgcgtgacg gacggcaagc agacgctctt cttctcatcc gatttcatgc ccgaccggca
     2821 ccacctcccg ctgccgtgga tcccgtcgca ggacctctat ccgctggaga cgctcgcggc
     2881 gaagaaggtg atccttccgc gtgccatcgc ggagcgctgg atcgtcgcgt tcacgcacga
     2941 cgtgccgcgc ttcggccaca tcaccgagca ggagggccgc taccgcttcg acgagctcgc
     3001 ggagtgaccg gggccgagga gcgcctcgca tgagcgagcg cgcgtcgacc ctcatcgtcg
     3061 gcggcggcat cgtgggcgcg gcggtcgcgt acttcctcgc cgagcggcgc gaaacggacg
     3121 tcgtcctgct cgagcgtggc gagctcggga gcggcaccac cgcgggcggg ctcggcggca
     3181 tccggcacca gttcgtcgac gagctcgacg tgcgcctctc catgctcgcc gcgaagacct
     3241 ggcgcgtctt cgacgagctc accggtgccc accacgactt ccgccagcgt ggctacctct
     3301 tcctcgcgaa caccgatgcg ggcctcgcag agctgcgtgc cgcgatgccg ctgtacgagc
     3361 gggtcggcgt gaacgtcgag atggtggacc cggtgacgat cgccgacctc gtccccggta
     3421 tgcgcgtgga cgatctccgc ggcgggcgcc tcggcgcgga ggacggatac ggcgatccgc
     3481 tccaggcgct cgcggggttc gcggccttcg cgcagctcgg cggcgtgcgg ttgcgggagc
     3541 acgtcacggt gcgcgatctg ctgcgcacgg gcgaccgcgt gaccggcgtc cgcaccgacg
     3601 ccggcgacat caccgccgat cgcgtgctgc tcgcgaccgg gtgct
//
LOCUS       DASTZR010000411         2762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_776004, whole genome shotgun sequence.
ACCESSION   DASTZR010000411 DASTZR010000000
VERSION     DASTZR010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776004"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..1443)
                     /gene="hisS"
                     /locus_tag="VFV20_09125"
     CDS             complement(76..1443)
                     /gene="hisS"
                     /locus_tag="VFV20_09125"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEU5288552.1"
                     /translation="MPSYQATRGMRDLLPEEAAAFDALQAVVEARALRYGYPRIITPV
                     VEDRSVFIKTTGESSDIVGKEMYDVTLGGEGSLTLRPEGTPAVARAFLDRGLHKAVRP
                     QRFFYFEPMFRGQRPQKLRFRQFWQWGLECFGAEEPAADIEIIEFTHGLMQEVGLTGY
                     EVRVNTIGDAKCRAKLREALTGYFRQHIDALSEASKRRVDTNVLRVLDSKEPQDRPVV
                     EGAPRILDLLCEDDRAHFDAVQNGLTALGIPFRVVPTLVRGLDYYTRTVTEFHLTAPE
                     YEGIAVAGGGRYDGLFATMGAEEMAGTGIAGGVDVLYYALQQEGVKVAEEPKADVYVI
                     SAQPDDGADRLAVAAGLRAGGFSVAIDYSKRALDKQLESAVKHGARVAVIRGTPEARG
                     GNAIVRDLIKKEQRVTKLAAVVKQVGRHVKPRAIPTLWRPPTDPGDQEPGAAGEAPYL
                     TDPRD"
     gene            1480..2043
                     /locus_tag="VFV20_09130"
     CDS             1480..2043
                     /locus_tag="VFV20_09130"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU5288553.1"
                     /translation="MDRRSLLRGATAGIVLAACGTTGSTAGSGGKASSTPGGIATNTP
                     TPAKATKATVELAKGGSFTFTLRPDKAPQTVDRFAGKARAGFYNNLTFHRVEDWVVQG
                     GDPKGTGTGGERVPSEYNDLTFKVGAVGIARGQDPAFNNDSQWFICKKDSTFLDTQYT
                     NFGQVTTGMEIVNGIKIGDKIKSITIE"
     gene            2043..2753
                     /locus_tag="VFV20_09135"
     CDS             2043..2753
                     /locus_tag="VFV20_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288554.1"
                     /translation="MLSARGTVALLVAFASFVAVALPLDRGTDAIQQSVLGLSAWIFL
                     AIALRLQPREIRTQVLVLVALATTLEVIGSIVWGAYRYRLENLPLYVPAGHGLFYLSA
                     LRAASLPIVRRHERTVVFAVAVGATLYMLRNLVAPALPDLLGLVTWAVFVRFIVRGTA
                     PALYAVSFVMTTALELYGTALGIWTWSPVLPVLMLPAANPPSGIGAGYAAMDALTRRI
                     VRRLRERHAASPELVEAA"
BASE COUNT          405 a         1015 c          896 g          446 t
ORIGIN      
        1 gtccccgcgt cggtggcgcg caactctcca caggtcttcg tacggagcat ctagtcccct
       61 tcaacgccgg gcacgtcagt ccctcgggtc cgtgaggtac ggtgcctcac ccgcggcacc
      121 gggctcctgg tcaccgggat ccgtcggcgg ccgccagagc gtcgggatcg cccgcggctt
      181 cacgtgacgc ccgacctgct tcacgaccgc ggcgagcttg gtcacgcgct gctccttctt
      241 gatcaggtcg cggacgatag cgttgccgcc gcgcgcctcg ggtgttccgc ggatcaccgc
      301 gaccctcgcg ccgtgcttca cggcgctctc gagctgcttg tcgagtgcgc gcttcgagta
      361 gtcgatcgcg acgctgaagc ctccggcacg cagtccggcg gccaccgcga ggcgatcggc
      421 gccatcgtcg ggctgcgccg agatcacata cacgtcggcc ttcggctcct cggccacctt
      481 cacgccctcc tgctgcaacg cgtagtacag gacatccacg cccccggcga tgccggtccc
      541 ggccatctcc tcggcgccca tcgtcgcgaa gaggccgtcg taccgccccc caccagcgac
      601 cgcgatgccc tcgtactccg gcgcggtcag gtggaactcg gtcaccgtgc gcgtgtagta
      661 gtcgaggccg cgcacgagcg tcggcacgac gcggaacggg atgccgagcg cggtgagccc
      721 gttctgcacc gcgtcgaagt gcgcgcggtc gtcctcacag agcaggtcga ggatccgcgg
      781 cgcgccctcg acgaccggcc ggtcctgcgg ctccttcgaa tcgagcaccc gcaggacgtt
      841 cgtgtcgacc cgccgcttcg atgcctcaga gagcgcgtcg atgtgctggc ggaagtagcc
      901 ggtcagcgct tcgcgaagct tcgcgcggca cttcgcgtcg ccgatcgtgt tgacgcggac
      961 ctcgtacccg gtgaggccga cctcctgcat gaggccatgg gtgaactcga tgatctcgat
     1021 gtccgcggcg ggctcctccg ccccgaagca ctccaggccc cattgccaga actggcggaa
     1081 ccgcagcttc tgcggccgct ggccccggaa catcggctcg aagtagaaga agcgctgcgg
     1141 gcgcaccgcc ttgtgcagcc cccggtcgag gaacgcgcgc gcgaccgccg gcgtcccctc
     1201 cggtctgagt gtgagcgagc cctcgccacc gagggtcacg tcgtacatct ccttgccgac
     1261 gatgtccgac gactcgcccg tggtcttgat gaacacgctc cggtcctcga cgaccggcgt
     1321 gatgatccgt ggatagccgt agcgcagcgc ccgcgcttcg acgacggcct gcagcgcgtc
     1381 gaacgcggcg gcctcttcgg gcaggaggtc ccgcatgccg cgggtcgcct gatagctcgg
     1441 caccggaaag atgacgataa accggtatcg tcggacgtga tggaccgacg atcgctcctg
     1501 cgcggcgcca cggccggcat cgtcctggcc gcgtgcggta cgacgggtag caccgccggc
     1561 tcaggaggca aggcctcatc aacaccggga gggatcgcca cgaatactcc gacgcccgcg
     1621 aaggcgacca aggccaccgt cgagctcgcg aagggcggat cgttcacgtt cacgctccgt
     1681 cccgacaagg cgccccagac cgtcgatcgc ttcgccggca aggcgagagc cggcttctac
     1741 aacaacctca cgttccaccg cgtcgaggac tgggtcgtcc agggtggcga tcccaagggc
     1801 accggcaccg gcggcgagcg cgtgccgagc gagtacaacg atctgacgtt caaggtcggc
     1861 gcggtcggca tcgcgcgcgg ccaggatccc gccttcaaca acgacagcca gtggttcatc
     1921 tgtaagaagg actccacctt cctcgacacg caatacacga acttcggcca ggtcacgacc
     1981 ggcatggaaa tcgtgaacgg catcaagatc ggcgacaaga tcaagagcat cacgatcgag
     2041 tagtgctgtc ggcgcgcggc accgttgcgc tcctcgtcgc gttcgcgtcg ttcgtcgccg
     2101 tcgcgctgcc gctcgaccgc ggcaccgatg cgatccagca gagcgtgctc gggctctcgg
     2161 cctggatctt cctcgccatc gcgctccggc tgcagccccg cgagatccgg acgcaggtgc
     2221 tcgtcctcgt ggcgctcgcc accacgctcg aggtcatcgg ctcgatcgtg tggggcgcgt
     2281 accggtaccg cctcgagaac ctgccgctct acgtacccgc agggcacggg ctcttctatc
     2341 tctcggcgct gcgcgccgcg tcgctcccca tcgtccgtcg ccacgagcgc acggtcgtgt
     2401 tcgcggtcgc cgtcggcgcg actctctaca tgctccgcaa cctcgtcgcg ccggccctcc
     2461 ccgacctgct cgggctggtc acgtgggccg tgttcgtccg cttcatcgtc cgcgggaccg
     2521 cgcccgcgct ctacgccgtc tcgttcgtca tgaccacggc gctcgagctg tacggcaccg
     2581 cgctcggcat ctggacgtgg tcgccggtgc tacccgtgct catgctcccc gcggcgaatc
     2641 cgccgagcgg gatcggcgcc ggctatgccg cgatggacgc gctgacgcgg cgcatcgtcc
     2701 ggcgtctgcg cgagcggcac gccgcgtcac cggagctggt cgaggcggcg tgacctagcg
     2761 ac
//
LOCUS       DASTZR010000412         3530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_153256, whole genome shotgun sequence.
ACCESSION   DASTZR010000412 DASTZR010000000
VERSION     DASTZR010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153256"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..393
                     /locus_tag="VFV20_09140"
     CDS             106..393
                     /locus_tag="VFV20_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288555.1"
                     /translation="MDRLVLAITAENADALLDGTRDADHRTLPPARLPAKAYLAVVGT
                     ASVVGECVLGERCGHTKDGWTLPVTAARRYKRPKPIAGYGLAKIPRSFRYL"
     gene            complement(499..831)
                     /locus_tag="VFV20_09145"
     CDS             complement(499..831)
                     /locus_tag="VFV20_09145"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5288556.1"
                     /translation="MPDPLEAARTFMDALEARDAERAASVCTEDVQIELPGGDSELQG
                     KDGARRLVKMAPAFVRRIREQETRGNTVILRGLTRSPGQFANYSTWTFETEDGLIKHV
                     TFSWRHAN"
     gene            850..3024
                     /locus_tag="VFV20_09150"
     CDS             850..3024
                     /locus_tag="VFV20_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288557.1"
                     /translation="MGQARGLIPYAAFAVFIVGATIVALVATVLAGPPAAAPAAAASP
                     AADATAAARPTTDLSPTGRIAYWRSEANGDYLLWVANADNSRRRSVAKTDQPNAVART
                     RWSFDGGSVAYVESGVRLVAVRVDGVATSYTLAPEVRAGGYRIVDHRFSPSGARIAAT
                     VQRTNGAQTDVYVSGPGGTWNRLTTSEDVLAADWISEDELLVQTVGGIIGRLRVAGRD
                     QLRPITALRGMGPVIGDDGRIHFLAPFTGPNDAVFGSSPIVYSINVDGEDLRTDNVTV
                     PSDSAWLDGIWPGGAYLVHRGISTAQALFGTAGEQVIPTSAGIIERLQVSPDRRFAIG
                     FTGSNLVRLDLAQGGAIANVVVLLGSIGPGDAWFARTRTLARSTRPNVDVPAARYAFA
                     LGGYVWVMGSDGEPTVLRSASANGQIRRGFTSLPAPRWSPGGDRLLTLESLSPGSPSF
                     QLIAVAIARDGTVRRYTSPSSVGTTLSWSPDGSQFAATTFTTPATDPSVLQSDLAISL
                     VDVSSGTVARTLPGREAYWTRAGIVVLSNGTYRGGSAARDDQALELVANNNEKRVIVA
                     LGSLVADPRMQAPSVTRGVTQASGLTASTDGAYAAIHVSFLSGNSGRAGFAIVRSRDG
                     TPTVVVARDGVADEAWSPNGRLVGYTLITAQGSGTRRSAVVRDAETGDIVQETDGRFA
                     GWSPDGSWTYVARNEGLYARRLAGGDPVRFSPYGVVVAVTRP"
     gene            complement(3011..>3530)
                     /locus_tag="VFV20_09155"
     CDS             complement(3011..>3530)
                     /locus_tag="VFV20_09155"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5288558.1"
                     /translation="EFVGLNERFDTRAKRYSEQIKVLRMLFANEVVDFTGTYHRIDRA
                     GIRPLPVRRAIPIWMGGNAEDAAKRIARISDGWFTFAQPDGDGPERIARFREYVRAAG
                     RDPETFPIEGRVVFAKRTPDEWVSRARGFADLRTTHLEVNTMGAGYATLADHLEALAR
                     FRRDAAELFTAS"
BASE COUNT          485 a         1263 c         1255 g          527 t
ORIGIN      
        1 cgccgtccga gcgagagacg ctcacgtccg tgatggagcg catcgcggcc gcggagtgac
       61 gcaagccgcc gccgatgtcc gttcgaggca cacccggagg tggacatgga caggctcgtc
      121 ctcgcgatca cggcggagaa cgccgacgct ctcctcgacg gcacccgcga cgccgaccat
      181 cggaccctcc cgccggcacg cctgccggcg aaggcatacc tcgccgtcgt cggtacggcg
      241 tcggtcgttg gcgagtgcgt gctcggggag cgctgcgggc acaccaagga cggctggacg
      301 ctcccggtga ccgcggcgcg tcgctacaaa cggccgaagc cgatcgccgg ctatggactc
      361 gcgaagatcc cccgctcgtt ccgctatctc tgatcagagt cgaacgggag gggctttgcc
      421 ccgacgagcg agtccgacag cgagcggagc gaggcggcga gccgcaggcc gcgtagcggg
      481 ccgaggcgag cgcgaagact agttcgcgtg ccgccagctg aacgtcacgt gcttgatgag
      541 gccgtcctcg gtctcgaagg tccaggtgct gtagttcgcg aactggccgg gcgagcgcgt
      601 cagcccgcgg aggatcaccg tgttgccgcg ggtctcctgc tcgcggatcc ggcggacgaa
      661 cgcgggcgcc atcttcacga gccggcgcgc gccatccttg ccctgcagct cgctgtcgcc
      721 accgggcagc tcgatctgca catcctcggt acagacgctg gcagcgcgct cggcgtcgcg
      781 cgcctcgagg gcgtccatga atgtgcgggc agcctctaac ggatcgggca taggagtgat
      841 tatgtagacg tggggcaggc ccgcgggctc ataccctacg cggcgttcgc cgtcttcatc
      901 gtcggcgcga cgatcgtcgc gctcgtggcg accgtgctgg cgggcccgcc ggccgccgcg
      961 ccggccgcgg cggcgagccc cgccgcggac gccaccgccg cagcccgacc caccaccgac
     1021 ctctccccga ccggccggat cgcgtactgg cggtctgagg cgaacggtga ctacctcctc
     1081 tgggtcgcca acgccgacaa ctcgcggcgg cgctcggtgg caaagacgga tcagccgaac
     1141 gccgtggcgc ggacgaggtg gtcgttcgac ggcggctcgg tcgcctacgt cgagagcggc
     1201 gtgcggctcg tggcggtgcg cgtcgacggc gtcgcgacct cgtacacgct ggcgcccgag
     1261 gtccgcgccg gtggctatcg catcgttgac caccgcttct cgcccagcgg ggcgcgcatc
     1321 gccgcgaccg tccagcgcac gaacggcgcg cagacagacg tctacgtgag cggtcccggc
     1381 gggacatgga accggctgac gacgtccgag gatgtcctcg ccgcggattg gatctcggag
     1441 gacgagctcc tcgtgcagac cgtcggcggc atcatcgggc gtctccgcgt cgccggacgc
     1501 gatcagctgc gtcccatcac cgcgctgcgg ggcatggggc ccgtcatcgg cgacgacgga
     1561 cgcatccact tcctcgcgcc gttcacgggg ccgaacgacg ccgtgttcgg atcgtccccg
     1621 atcgtgtaca gcatcaacgt cgacggggag gatcttcgaa cggacaacgt caccgtccca
     1681 tccgatagcg cctggctcga cgggatctgg ccgggcggag cctatctcgt ccaccggggc
     1741 atcagcaccg cgcaggcgct cttcggcacg gccggggagc aggtcatccc cacgagcgcg
     1801 ggcatcatcg agcggctcca ggtgtcgccc gaccgccgct tcgccatcgg attcaccggc
     1861 tcgaacctcg tccggctcga cctcgcgcag ggcggcgcga tcgcgaacgt cgttgtgctc
     1921 ctcggctcga tcggcccggg cgacgcctgg ttcgcgcgaa cgcgcacgct cgcgaggtcc
     1981 acgcggccga acgtcgacgt gcccgccgcg cgctacgcgt tcgcgctcgg cggatacgtg
     2041 tgggtcatgg gctcggacgg cgagcccacg gtgctgcggt cggccagcgc gaacggccag
     2101 atccgtcgcg gattcacgtc cctcccggcg ccacggtggt cgccgggcgg cgatcgcctg
     2161 ctcacgctcg agtcgttgtc gccgggctcg ccgagcttcc agctcatcgc ggtcgcgatc
     2221 gcgcgtgacg gcaccgtgcg ccggtacacc tcgccctcgt ccgtcggcac gacgctctca
     2281 tggtcgcccg acgggtcgca gttcgcggcg accaccttta cgacgcccgc gaccgatccc
     2341 tccgtgctcc agagcgacct cgcgatctcg ctcgtcgatg tctcctcggg cacggtcgcg
     2401 cggacgctgc ccgggcgcga ggcgtactgg acgcgtgcgg ggatcgtggt gctgtcgaac
     2461 ggcacgtatc gcggcggttc cgcggcgcgc gatgaccagg cgctcgagct cgtcgcgaat
     2521 aacaacgaga agcgcgtgat cgtggcactg ggcagtctgg tcgccgatcc ccgcatgcag
     2581 gctccgtcgg tcacgcgcgg ggtgacgcag gcgagcgggc tgaccgcgtc gacggacggc
     2641 gcatacgcgg cgatccacgt gtcgttcctc tcggggaact cgggccgggc cgggttcgcg
     2701 atcgtgcgat cgcgcgacgg taccccgacc gtcgtcgtcg cgcgcgacgg cgtggccgac
     2761 gaggcatggt cgccgaacgg tcgcctcgtc ggatacaccc tgatcacggc tcaggggtcc
     2821 ggcacccgca gaagcgcggt cgtgcgggac gcggagaccg gcgacatcgt gcaggagacc
     2881 gacggtcggt tcgccggctg gtcccccgac ggctcgtgga cgtacgtcgc gcgcaacgag
     2941 ggcctctacg cgcggcgcct cgctggaggc gacccggtgc gcttctcgcc ctacggcgtg
     3001 gtggtcgcgg tcacgaggcc gtgaacagct cggccgcgtc gcggcggaat cgggcgagcg
     3061 cctcgaggtg gtcggccagc gtcgcgtagc ccgcgcccat ggtgttgacc tcgaggtgcg
     3121 tcgtgcgcag gtcggcgaag ccgcgcgctc tgctcaccca ctcgtccggc gtgcgtttcg
     3181 cgaagacgac gcgtccctcg atggggaacg tctcgggatc gcgacccgcc gcacggacgt
     3241 attcgcggaa gcgtgcgatc cgctccggcc cgtcgccatc gggctgcgcg aaggtgaacc
     3301 agccgtcgct gatgcgcgcg atgcgcttcg ccgcgtcctc cgcgttcccg cccatccaga
     3361 tcgggatcgc ccgccggacc gggagcggcc tgatgcccgc gcgatcgatg cggtggtacg
     3421 tcccggtgaa gtcgaccacc tcgttcgcga agagcatgcg cagcaccttg atctgctcgc
     3481 tgtagcgctt cgcgcgggtg tcgaagcgct cgttcagtcc gacgaactcc
//
LOCUS       DASTZR010000413         5789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_674402, whole genome shotgun sequence.
ACCESSION   DASTZR010000413 DASTZR010000000
VERSION     DASTZR010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5789
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674402"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3398)
                     /locus_tag="VFV20_09160"
     CDS             complement(<1..3398)
                     /locus_tag="VFV20_09160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU5288559.1"
                     /translation="MPRSFHQRRREGSAVQQFVHLHTHSEYSLLDGLSRVTDLVSRAK
                     ELGMPALALTDHGALYGAIDFYVQASQAGIKPIVGVEAYVARTNRFERDPRIEGHGKP
                     FHLVLLAKDFTGYQNLVALVTAAHLDGYYYRPRMDKDILRERAKGLIALSSCLQGEVA
                     RAILEEGVDAACRVALEHQEIFGAGNYFLELMDHGIPEQERQREGVREVARRTKIPMV
                     VTNDLHYVHAEDAEAQDVMVCIQQGKTIEATDRLKMIDHPELYLKSAEEMYARFPEDH
                     EALENTLRIASLVDLKLPLGEVKMPHFEVAAGLTPEGQLLKMAEVGIAKKYGAMTPDL
                     RARLDKEIDVIGKAGYAGYILIVQDFIRYAQENGILTAVRGSAGGSLVNYAIGLTDID
                     PIKYGLIFDRFLNLERYTPPDIDVDFMDNRRDEVIAYVTKKYGADRVAQIATFNTMLA
                     RAAVRDVARVLGMPYGEADRVAKVIPFGVDLQEARRMVVELRDMEQQPDIVRLLDLAE
                     KVEGLVRSTGTHAAGVVITREPLTNLVPLERSKGKAEALQSQFEDKSLDKLGVLKFDF
                     LGLGNLTILYEAMRLIREKRGVTVERSQIPLDDKKTYELLGSGETTGLFQLESAGMRR
                     HIRDLKPDRIEDVMAMVALFRPGPMDYIPTYIRRKHGQEPVTYAHPSLEPVLRSTYGV
                     MVYQEDVMAVTQALAGFTMGEADVLCYTIRKKIKEKLDAQKSKFVQGAIRQGVHAETV
                     EQVWKDFEPFARYGFNRAHAAIYGLIAYHTAYLKANYSVEYMTAVLSAEMGVPERVAI
                     AVAECRRMGIAVLPPDVNESDFGFTITASGIRFGLGAVKNVGEGAVESIIEARRGGGP
                     FASLDDLCARIDLQRCNKRVLESLVKCGATPAFGPRANQIARLDAVMATAQQEQEAAR
                     QGQVSLFADLGATLSFKGDLLATHVPGTAEAPRKDLLSWEKELLGIYLSDHPLQDVAA
                     RMGDVVTAYLAELKEEEEQGLVIVACVVTSARKHITSKEKKLMMFAQVEDLTGSVEAT
                     IFPRTYDATAAVWGTDEILLVLGRVEKRDDAPKLLVEHAVRFNDAGIEEIKRVYRESA
                     ERFAKRARFMKPGNGETNGGPARGPAPSAQARPASPVETAPASIP"
     gene            complement(3422..5260)
                     /locus_tag="VFV20_09165"
     CDS             complement(3422..5260)
                     /locus_tag="VFV20_09165"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU5288560.1"
                     /translation="MIGEVTFGVIGLLFLLADVRTFASTFQQSFFGAPNTNLAVMATA
                     VFATSFLGLLVAWRFRPARALGTSGVVFAVATVLLTASRHNWVDLGLAVIALAAGFWW
                     LAFLHSARVEGTSSPLAIAVPIAFAADLALRSLFRTVAAPDLAWPTAFGLAIVGLVVF
                     GAAGLVTIAPPRQWTGPDLRGALGLLALPALLLVAETGGTNGAQAALAAGWGLGPEPA
                     GATQTGAAALGAGLAVGLLSAWRLPLRGPIAALLLAGGAALLWAHLPYASLVGGAVLA
                     AACVLAAPALLGGRLRPSRAPISVVVALSVGWLLFVGAAFGFYAFWAYYPALWAATAF
                     VVLATLVAPIPRVRPSFALTLAGCLLAVGAPLAARAATPAPADVEAARNTFRLMTYNI
                     HQGFNAGQIPSLDELAGVIGAESPDVLCLQEVARGWMVDEGHDSLSVLAERLGMRYAW
                     FPAIGDLYGDAILSRFEMTDVKRVRYAIEPGAKHQPRGAISVKTSGVTIVCTHLDDVS
                     DSTSVRQEQVRTILREWIDVTPIAIAGDMNGTPDSIEIRLYDQSGFDDLGADAGPTTT
                     MDDPQKRIDYVFGRGLIGGQAHTVPLDVTMRASDHRGLVVNVTISK"
     gene            complement(5280..>5789)
                     /locus_tag="VFV20_09170"
     CDS             complement(5280..>5789)
                     /locus_tag="VFV20_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288561.1"
                     /translation="AEPVRGAEDILRDLLRRCAGAGFATAFLVGVDPGAGTLRGELVS
                     DGEAAREVDYALGISSGKFAVPLDDRYNAIARAVREARIVPAPTAYEITRPATDFDGA
                     QAIERLARGGRSVTLPMIVSGEAAGALVLGPMADDPTFSAIEAVRGYLADAAAELGAL
                     WGTHDTQGA"
BASE COUNT          871 a         2025 c         2030 g          863 t
ORIGIN      
        1 ggtatcgatg ccggtgcggt ctcgaccggc gaggcaggcc gcgcctgcgc cgatggcgcg
       61 ggcccgcggg ccggcccgcc gttcgtctcc ccgttgccgg gcttcatgaa tcgcgcacgc
      121 ttcgcgaagc gctcggcgct ctcgcgatac acgcgcttga tctcctcgat acccgcgtca
      181 ttgaaccgca ccgcgtgctc cacgagcagc ttcggcgcgt cgtcgcgctt ctcgacgcgg
      241 ccgagcacga ggaggatctc gtcggtgccc cagaccgccg cggtggcgtc gtaggtgcgc
      301 gggaagatcg tggcctcgac ggatccggtg aggtcctcga cctgcgcgaa catcatgagc
      361 ttcttctcct tgctcgtgat gtgcttccgc gcgctcgtca cgacgcacgc gacgatgacg
      421 aggccctgct cctcctcctc cttgagctcc gccaggtacg ccgtcaccac gtcgcccatg
      481 cgcgctgcca cgtcctggag cggatggtcg gacaggtaga tcccgaggag ctccttctcc
      541 catgagagaa ggtccttgcg cggcgcttcc gccgtgcccg ggacgtgcgt cgcgagcaga
      601 tcgcccttga acgagagcgt ggcgcccagg tcggcgaaga gcgacacctg cccctgccgc
      661 gccgcctcct gttcctgctg cgccgtcgcc atcaccgcgt cgaggcgcgc gatctggttc
      721 gcgcgcggac cgaaggccgg cgtcgcaccg cacttcacga gcgactcgag cacgcgcttg
      781 ttgcagcgct ggaggtcgat gcgcgcgcag agatcgtcga gcgaggcgaa cggaccaccg
      841 ccgcggcgcg cctcgatgat gctctcgacg gcgccctcgc cgacgttctt caccgcgccg
      901 aggccgaacc ggatcccgga cgcggtgatg gtgaagccga agtccgactc gttgacatcc
      961 ggaggcagca cggcgatgcc catacgccgg cactccgcca ccgcgatggc cacgcgctcg
     1021 ggcacgccca tttccgcgga gagcaccgcg gtcatgtact cgacggaata gttcgccttg
     1081 agatacgcgg tgtggtacgc gatgaggccg tagatcgcgg cgtgcgcgcg gttgaacccg
     1141 taccgcgcga acggctcgaa gtccttccag acctgctcaa cggtctcggc gtgcacgccc
     1201 tggcggatcg cgccctgcac gaacttcgac ttctgcgcgt cgagcttctc tttgatcttc
     1261 ttgcggatcg tgtagcagag aacgtcggcc tcgcccatcg tgaagccggc gagcgcctgc
     1321 gtcaccgcca tcacgtcctc ctggtagacc atgacgccgt aggtcgagcg aagcacgggc
     1381 tcgagactcg ggtgcgcgta ggtgacgggc tcctgtccat gcttgcggcg gatgtacgtc
     1441 gggatgtagt ccatcggtcc cgggcggaag agcgcgacca tggccatgac gtcctcgatg
     1501 cggtcgggct tcaggtcgcg gatgtggcgc cgcatgcccg ccgactccag ctggaacagt
     1561 ccggtcgtct cgccggagcc cagcagctcg taggtcttct tgtcgtcgag cgggatctgc
     1621 gagcgctcga ccgtgacgcc gcgcttctcc ctgatgagcc gcatcgcctc gtagaggatc
     1681 gtcaggttgc cgaggccgag gaagtcgaac ttcaggacgc cgagcttgtc gagcgacttg
     1741 tcctcgaact ggctctgcag cgcttctgcc ttgcccttcg agcgctcgag gggcacgagg
     1801 ttcgtgagcg gctcgcgcgt gatgacgacg cccgcggcgt gcgtgccggt ggagcgcacg
     1861 agcccctcga ccttctccgc gaggtcgagc agtcgcacga tgtcgggctg ctgctccatg
     1921 tcgcgcagct cgacgaccat gcggcgcgcc tcctggaggt cgacaccgaa cgggatcacc
     1981 ttcgcgacac ggtcggcctc gccgtacggc atgccgagca cgcgcgcgac gtcccggacg
     2041 gccgcgcgcg cgagcatcgt gttgaacgtg gcgatctgcg cgacgcggtc cgcgccgtac
     2101 ttcttcgtga cgtacgcgat gacctcgtcg cggcggttgt ccatgaagtc cacgtcgatg
     2161 tccggcgggg tgtaccgctc gaggttgagg aagcggtcga agatgagccc gtacttgatc
     2221 ggatcgatgt ccgtcagccc gatcgcgtag ttcacgagcg agccgccggc gctcccgcgc
     2281 acggcggtca ggatcccgtt ctcctgcgcg tagcggatga agtcctgcac gatgaggatg
     2341 tagccggcgt agccggcctt cccgatgacg tcgatctcct tgtcgagccg ggcgcgcagg
     2401 tccggcgtca tcgcgccgta cttcttggcg atccccacct ccgccatctt cagcagctgg
     2461 ccctcgggcg tgagacccgc cgccacctcg aagtgcggca tcttcacctc gccaaggggc
     2521 agcttgaggt cgaccagcga cgcgatgcgc agcgtgttct cgagcgcctc gtggtcctcg
     2581 gggaaccgcg cgtacatctc ctccgcgctc ttcaggtaca gctcggggtg gtcgatcatc
     2641 ttgagccggt cggtcgcctc gatcgtcttg ccctgctgga tgcagaccat gacgtcctgc
     2701 gcttccgcat cctcggcgtg cacgtagtgg aggtcgttgg tgacgaccat cgggatcttc
     2761 gtgcggcggg ccacctcgcg cacgccctcc cgctggcgct cctgctccgg gatgccgtgg
     2821 tccatcagct cgaggaagta gttgcccgcg ccgaagatct cctggtgctc gagcgcgacg
     2881 cggcaggccg cgtcgacgcc ctcctcgagg atcgcgcgcg ccacctcgcc ctggaggcag
     2941 gacgagagcg cgatgagacc cttggcgcgt tcgcgcagga tgtccttgtc catgcgcggg
     3001 cggtagtagt agccgtcgag gtgggcggcg gtgacgaggg cgaccaggtt ctggtagccg
     3061 gtgaagtcct tcgcgaggag caccaggtgg aacggcttgc cgtggccctc gatccgcggg
     3121 tcgcgctcga agcgattcgt gcgcgcgacg tacgcctcga cgccgacgat cggcttgatc
     3181 cccgcctggc tcgcctgcac gtagaagtcg atcgcgccgt acagcgcccc gtggtcggtg
     3241 agcgcgagcg cgggcatgcc gagctccttc gcccgcgata cgaggtcggt cacgcgggac
     3301 agcccgtcga ggaggctgta ctcgctgtgc gtgtggaggt gaacgaactg ctggacggcg
     3361 gacccctcac ggcgccgctg gtggaagctc cgcggcaaag agcagtctac ccggcgagga
     3421 attacttcga gatggtcacg ttcacgacga ggccgcgatg gtcgctggcg cgcatggtca
     3481 cgtcgagcgg cacggtgtgc gcctgcccgc cgatgaggcc tctcccgaag acgtagtcga
     3541 tccgcttctg gggatcgtcc atcgtcgtcg tcggccccgc gtcggcgccg aggtcgtcga
     3601 agccggactg gtcgtacagg cggatctcga tggagtccgg cgtgccgttc atgtcaccgg
     3661 cgatcgcgat cggggtcacg tcgatccact cgcggaggat cgtgcgcacc tgctcctgcc
     3721 ggacggaggt gctgtccgac acgtcgtcga gatgcgtgca cacgatcgtc acgccggacg
     3781 tcttcacgga gatcgcgccg cgcggctggt gcttcgcgcc gggctcgatc gcgtagcgca
     3841 cgcgcttcac atccgtcatc tcgaagcgcg acaggatcgc gtcgccgtag aggtcgccga
     3901 tcgccgggaa ccacgcgtag cgcatgccga gacgctcggc gagcaccgac agcgagtcgt
     3961 gcccctcgtc caccatccag ccgcgcgcga cctcctggag gcagagcacg tcgggcgact
     4021 ccgcgccgat gacgccggcg agctcgtcga gcgacgggat ctggccggcg ttgaagccct
     4081 ggtggatgtt gtaggtcatg agtcggaacg tgttgcgcgc ggcctccacg tcggccggag
     4141 cgggtgtcgc ggcgcgcgca gccagcggcg cgccgaccgc gaggaggcag ccggccagcg
     4201 tgagcgcgaa gctcggccga acgcgcggga tcggagcgac gagcgtggcg agcacgacga
     4261 aggcggtcgc ggcccacagc gccgggtagt acgcccagaa cgcgtagaag ccgaacgcgg
     4321 cgccgacgaa cagaagccat ccgaccgaca gcgcgaccac gacggagatg ggcgcgcgcg
     4381 agggtcgcag acgtccgcca agcagcgccg gcgccgcgag gacacaggcg gccgcgagga
     4441 ccgcaccgcc caccagcgag gcgtacggca gatgcgccca caggagcgcc gcaccgcccg
     4501 cgagcagcag tgcggcgatc gggccgcgca gcggcaggcg ccaggcggac aggagtccga
     4561 ccgcgagccc agccccgagg gcggcggcac ccgtctgcgt ggcccccgcg ggctccggcc
     4621 caagtcccca tccggccgcg agcgcggcct gcgcgccgtt cgtcccgccg gtctccgcca
     4681 cgagcaggag cgcgggcagc gccagcagac cgagcgcgcc gcgcagatcg gggcccgtcc
     4741 actgcctcgg cggagcgatg gtcacaagcc ccgccgcccc gaacacgacg aggccgacga
     4801 tcgcgaggcc gaacgccgtc ggccaggcga ggtccggcgc ggccaccgtg cggaacagcg
     4861 atcgcagcgc gaggtcggcg gcgaacgcga tcggcaccgc gatcgcgagc ggcgacgagg
     4921 tgccctcgac gcgcgcgctg tgcaggaacg cgagccacca gaagccggcg gccagcgcga
     4981 tgaccgcgag cccgaggtcc acccagttgt ggcgcgacgc cgtgaggagc accgtggcga
     5041 ccgcgaacac cacgccgctc gtaccgaggg cgcgcgcggg gcggaaccgc cacgcgacga
     5101 ggagacccag gaacgacgtc gcgaacaccg ccgtcgccat cacggcgagg ttcgtgttcg
     5161 gcgcgccgaa gaacgactgc tggaacgtcg atgcgaacgt gcgcacgtcc gcgaggagga
     5221 acaggaggcc gatgacgccg aaggtcacct cgccgatcac gcggcgagtc tactgagcgc
     5281 tacgcaccct gcgtgtcgtg cgttccccag agcgcgccga gctccgcggc cgcgtcggcg
     5341 aggtagccac ggacggcctc gatcgcggag aacgtgggat cgtcggccat cggaccgagg
     5401 acgagcgcgc cggccgcctc tcccgagacg atcatcggca gcgtgacgga ccggccaccg
     5461 cgcgcgagcc tttcgatggc ctgcgcgccg tcgaaatcgg tcgccggccg cgtgatctca
     5521 tacgcggtcg gcgcgggcac gatgcgcgcc tcgcgcaccg cgcgcgcgat cgcgttgtag
     5581 cggtcgtcga gggggaccgc gaacttgccg ctcgagatgc cgagcgcgta gtcgacctcg
     5641 cgggctgcct cgccgtcgga cacgagctcg ccgcgcagcg tgcccgcacc gggatccacg
     5701 cccaccagga acgcggtcgc gaatcccgca ccggcgcacc gccgcagcag gtcgcggagg
     5761 atgtcctccg cgccgcgcac gggctccgc
//
LOCUS       DASTZR010000414         3192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_440737, whole genome shotgun sequence.
ACCESSION   DASTZR010000414 DASTZR010000000
VERSION     DASTZR010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440737"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VFV20_09175"
     CDS             <1..317
                     /locus_tag="VFV20_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288562.1"
                     /translation="YVAVGIWGIVLGIRNSGPTPGFRGGLFIAELAALSQGLIGILVF
                     FFLRGPSDPLHILYGVALALAIPLALSLVRERTPRGQSVALGFALLFAAGLALRGITT
                     AG"
     gene            314..1492
                     /locus_tag="VFV20_09180"
     CDS             314..1492
                     /locus_tag="VFV20_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5288563.1"
                     /translation="MSAAPEIDRTPDSSRDALQALDGDFFYFNVAGSGPTFPVAARMA
                     ERYRSWLSSVGMFSHVGYDAYNAALDATRADIADFVGDPGGAAHVALTQSATDALNTL
                     IGGLRLPPGSLLVTTAEEHGSALMPTFARRARGDRVAVVAHRDDPSFLGELDRAFSDG
                     AAALVISLVSCKSGKVLPVAAAARLAHARGAAVIVDCAQAAGQIAVDVRALGADAYAF
                     LGYKWLHGPLGVGALWIRDIERFDVLRMGWRSQTAIDLDGNITLRPDAARLETGTVDA
                     GAYVGLRQALAVHRALGESTARRIRALRARLLERLSELPFDVLSRAEDPTGIVVASPR
                     GTSSSEVVDAMWRDHRIVVKLLTEPGLDAIRISFWALHAEADIDRLAAGFAKTIRASV
                     "
     gene            1544..2332
                     /locus_tag="VFV20_09185"
     CDS             1544..2332
                     /locus_tag="VFV20_09185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5288564.1"
                     /translation="MTMLGGQVAIVTGGGRGLGRAIAETLAALGADVVIASRNAPELD
                     EVVRAIRRAGGHALAQTADVSDDRQVQELVLAAERWVGPASILVNNAGMAEPMEPLVR
                     SDPALWLRNISINIGGTYLPTRAALPGMLERGAGRIVNITSGAAKRPTPGWTAYSAAK
                     AGVVQLTRSLALELEGTGVSVCAYNPGPLDTEMQERIRRTRADDFPRADEYRQMQREG
                     RLRDPKDAARAIAYLTLPSTERNGQALDFDDADLARAVEAALPG"
     gene            2332..>3192
                     /locus_tag="VFV20_09190"
     CDS             2332..>3192
                     /locus_tag="VFV20_09190"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEU5288565.1"
                     /translation="MAAPPVRAVELERFESTFNLTRISGAVLTYLLGPFFPNIGIQHV
                     VLFGSLLLAQSAFVAFLLRSGRLRRHPEMLERVIFAIDLLTISYAIFLFAYDPNWTTY
                     IVGLLVVIAGGFRFASLGALVASLVMAATYVATAVYREVVFAYPTETQRIAFTVAIYV
                     IAGFLMAGLLRELGTLRAQREAFERQRAETEALRELDRMKSEFLATMSHDFRSPLTVI
                     RGNLELLQSQRPGALTDAQRDLATRAERNVHRLEEFTEDLLEMARIEHDAIVLDRDDI
                     DIAELIREVVD"
BASE COUNT          416 a         1154 c         1104 g          518 t
ORIGIN      
        1 actacgtcgc cgtggggatc tggggcatcg tgctcggtat ccgtaacagc ggaccgaccc
       61 cgggattccg cggcggcctc ttcatcgcgg agctggccgc gctgtcccag ggactgatcg
      121 gcatcctcgt gttcttcttc ctgcgcggcc cgtcggatcc gctgcacatc ctgtacggcg
      181 tcgcgctggc actcgcgatc ccgctcgcgc tctcgctcgt gcgtgagcgg acgccacgcg
      241 gacagtcggt cgcgctgggc ttcgccctgc tcttcgccgc gggtctcgcg ctccgcggca
      301 tcaccaccgc gggatgagcg cagcacccga gatcgaccgc acacccgaca gctcgcgcga
      361 cgcgctccag gcgctggatg gcgacttctt ctatttcaac gtggccggct ccggcccgac
      421 gttcccggtc gcggcgcgca tggccgagcg ctaccgctcg tggctctcca gcgtcggcat
      481 gttctcgcac gtcggatacg acgcgtacaa cgccgcgctc gacgcgacgc gcgccgacat
      541 cgccgacttc gtcggagatc cgggtggtgc cgcgcacgtc gcgctgacgc agtcggcgac
      601 ggacgcgctg aacacgctga tcggcgggct gcggctccca ccgggctcgc tgctggtcac
      661 cacggccgag gagcacggga gcgccctgat gccgacgttc gcgcggcggg cacgcggcga
      721 ccgggtggcg gtcgtggccc atcgcgacga cccctcgttc ctcggcgagc tggaccgcgc
      781 gttctcggac ggcgcggcgg cgctcgtgat ctcgctcgtc tcgtgcaaga gcggcaaggt
      841 gctgccggtc gcggccgcgg cccgcctcgc gcacgcgcgc ggcgccgcgg tgatcgtcga
      901 ctgcgcgcag gccgccgggc agatcgcggt cgacgtgcgc gcgctcggcg cggatgcgta
      961 cgcgttcctt ggctacaagt ggctgcacgg gccgctgggg gtgggcgcgc tctggatccg
     1021 cgacatcgag cgcttcgacg tcctgcgcat gggctggcgc tcgcagacgg ccatcgacct
     1081 cgacggcaac atcacgctcc gcccggacgc ggctcgcctc gagacgggga ccgtcgacgc
     1141 cggcgcgtac gtcggcctgc gccaggcgct ggcggtgcat cgggcgctcg gcgagtcgac
     1201 ggcgcggcgc atccgcgctc tgcgtgctcg gctgctcgag cggttgtccg agctcccgtt
     1261 cgacgtcctg tcacgggcag aggatccgac ggggatcgtc gtggcgtcgc cgcgcggcac
     1321 ctcgtcgtcc gaggtcgtgg acgcgatgtg gcgggatcac cgcatcgtcg tgaagctcct
     1381 gaccgagccc ggcctcgacg ccatccggat ctcgttctgg gcgctccacg ccgaggcgga
     1441 catcgaccgc ctcgccgcgg gcttcgccaa gaccattcgc gcgtcggtct gaggccgttc
     1501 gctcgcagtc gtcgctcccc attcggtcta tcctctgtcg cccatgacca tgctcggcgg
     1561 acaggtggcc atcgtcaccg gtggcggacg cggactcggg cgcgcgatcg ccgagacgct
     1621 cgcggcgctc ggcgccgacg tcgtcatcgc atcgcggaat gctcccgagc ttgacgaggt
     1681 cgtgagggcg atccggcgcg cgggcggtca cgcgctggca cagacggcgg acgtctccga
     1741 tgaccgccag gtgcaggagc tcgtgctcgc ggcggagcgc tgggtcggcc ccgcgtcgat
     1801 cctcgtcaac aacgccggca tggccgagcc gatggagccg ctcgtccgtt cggacccggc
     1861 gctgtggctg cgcaacatct ccatcaacat cggcgggacg tacctgccga cgcgcgccgc
     1921 gctgccgggg atgctcgagc gcggtgccgg gcgcatcgtg aacatcacct ccggcgccgc
     1981 gaagcgtccg acgccgggat ggaccgcgta ttccgctgcg aaggccgggg tggtgcagct
     2041 cacgcgaagc ctcgcgctgg agctcgaggg caccggcgtg agcgtgtgcg cgtacaaccc
     2101 ggggccgctc gataccgaga tgcaggagcg catacgtcgt acgcgcgcgg acgactttcc
     2161 ccgggccgac gagtaccggc agatgcaacg cgagggtcgg ctgcgcgacc cgaaggacgc
     2221 ggcccgcgcg atcgcctatc tcacgctccc ttcgaccgag cggaatggcc aggctctcga
     2281 cttcgatgac gccgacctcg cgcgcgccgt ggaggccgcg ctccccggtt agtggcagcc
     2341 cctcccgtcc gcgcggtcga gctcgagcgt ttcgagtcca cgttcaacct cacgcgcatc
     2401 agcggcgccg tcctcacgta cctgctcggc ccgttcttcc cgaacatcgg catccagcac
     2461 gtcgtgctgt tcggttcgct ccttctcgcg cagtccgcgt tcgtcgcgtt cctgctccgc
     2521 tcgggcagac tgcgacggca tcccgagatg ctcgagcgcg tcatcttcgc gatcgacctg
     2581 ctcacgatct cgtacgcgat cttcctgttc gcgtacgacc ccaactggac gacctacatc
     2641 gtcggcctgc tcgtcgtcat cgccggtggc ttccgcttcg cgagcctcgg cgcgctggtg
     2701 gcgtcgctcg tcatggcggc gacgtacgtc gcgacggcgg tctatcgaga ggtcgtcttc
     2761 gcctatccca ccgagacgca gcgcatcgcc ttcacggtcg cgatctacgt gatcgcggga
     2821 ttcctcatgg ccgggctgct gcgcgagctg ggcacgctgc gcgcgcagcg cgaggcgttc
     2881 gagcggcagc gcgctgagac ggaggcgctg cgcgagctcg atcggatgaa gagcgagttc
     2941 ctcgcgacga tgtcgcacga cttccgcagc ccactcacgg tgatccgcgg caatctcgag
     3001 ttgttgcaga gccagcggcc gggtgcgctc accgacgcgc agcgcgatct cgcgacccgc
     3061 gcggagcgca acgtccaccg gctggaggaa ttcaccgagg atctcctcga gatggcgcgc
     3121 atcgagcacg acgcgatcgt gctcgaccgc gacgacatcg acatcgccga gctgatccgt
     3181 gaggtggtcg ac
//
LOCUS       DASTZR010000415         2953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_649374, whole genome shotgun sequence.
ACCESSION   DASTZR010000415 DASTZR010000000
VERSION     DASTZR010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649374"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VFV20_09195"
     CDS             <1..583
                     /locus_tag="VFV20_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
                     /protein_id="HEU5288566.1"
                     /translation="RVALGDRELAGSVVRFPMVHGERDGQRRFYALWKRMADHRLAIL
                     MSEKATAFRSSRTHVDNAAYAVALVATDERAAGRVYNVGEPDALPWSEWARLVAEQVR
                     WGGQLVIRPSEEMPAHLRDSRPDMWDHHLVADTTRIRRELGYQEVVPREDGLRRALAW
                     YAEAPPEDPKAAGNLDYEAEDDALVTIRGSAVH"
     gene            592..1119
                     /locus_tag="VFV20_09200"
     CDS             592..1119
                     /locus_tag="VFV20_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5288567.1"
                     /translation="MGAVEAVRAVDAAPAIPPAFEALFRKEYARVVRIAQRIVRDADE
                     AEDVAQDVFVSFYRKHPADAGYASAWLHAAAAHTALNFVRSRERRGKRELAHTQERDV
                     AVDPARLAEDTETRAEVRAALARLDERSASLLALRYSGLSYDEIAAALGLRRSSIGTF
                     LSRAEAAFRKEVTRS"
     gene            1116..2120
                     /locus_tag="VFV20_09205"
     CDS             1116..2120
                     /locus_tag="VFV20_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288568.1"
                     /translation="MSEDVEKLLGIHEVEVDTERALTRIRARVERERLRPATFAVARR
                     DGALVSIWLKGLAAAASVALVAVLLTASGVAETILTIFEPKQIVAVPITTTDISTAAS
                     GLGSYGTLEFSSQPKPYDVPDLATARKDSGLTVLVPAQLPAGVSTAAARYGVMPRTTA
                     TFTFSAAKTQAAASRLGRTPPPMPANIDGSKLFITGGPVVVQYFGDGTVPTSATGNPF
                     AGMPRLVIAQGKGPIVQSDGVTVEQLQQYLLAQPGVSPQLAAQIRAIKDPSSTLPIPI
                     PVDMATSKNVTVQGQPGVFIGDSTGMGSALIWQKDGIVFGVAGTLTESQILAVANSLH
                     "
     gene            2117..>2953
                     /locus_tag="VFV20_09210"
     CDS             2117..>2953
                     /locus_tag="VFV20_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5288569.1"
                     /translation="MSETPAIRTRGLAKRYGSVQALAGLTMTIPRGAVFGFLGPNGAG
                     KTTAVKLLLGLTRPNGGDGEILGLPIGSAASRRRVGYLPELFRYQGWLTAREVLALHC
                     ELAGIDRAAWSREIEDALAIVGIADRGSTRVGGFSKGMQQRLGLGVALLGDPELVLLD
                     EPTSALDPVGRHDVREIIRALRERGVTVFLNSHLLSEVEQVCDRVAIVDRGRVVADGT
                     LDDLLRGDIVRIRATELPRDALASLERFGRLDADGEWLVARGIAEDRVPELVAEIVRI
                     GGR"
BASE COUNT          426 a         1083 c         1040 g          404 t
ORIGIN      
        1 gcgcgtcgcg ctcggcgacc gcgagctcgc cggcagcgtc gtgcgcttcc cgatggtgca
       61 cggcgagcgc gacggacagc gccgcttcta tgcgctgtgg aagcgcatgg ccgatcaccg
      121 cctcgccatc ctcatgagcg agaaggccac cgccttccga tcgtcgcgga cgcatgtgga
      181 caacgccgcc tacgcggtgg cgctcgtcgc caccgatgag cgcgccgcgg ggcgtgtcta
      241 caacgtcggc gagcccgacg cgctgccctg gagcgagtgg gcgcgcctgg tcgcggagca
      301 ggtccgctgg ggcggtcagc tcgtcatccg cccgtccgaa gagatgccgg cacacctgcg
      361 cgactcacgg cccgacatgt gggaccacca cctggtggcg gacacgacac ggatccggcg
      421 cgagctcggc taccaggagg tcgtcccccg cgaggacggc ctgcgccgcg cgctggcgtg
      481 gtacgccgag gcgccgccgg aggacccgaa agcggccgga aacctcgact acgaggccga
      541 ggacgatgcg cttgtcacga tccgcggctc ggcggtgcat tgaaagaaga catgggcgcc
      601 gtcgaggccg tccgagcggt ggacgccgct ccggccatac cacccgcgtt cgaggcgctc
      661 ttccggaagg agtacgcccg cgtggtgagg atcgcgcagc ggatcgtgcg tgatgccgac
      721 gaggctgagg acgtcgctca ggacgtcttc gtgtccttct atcggaagca ccccgccgac
      781 gccgggtacg cctcggcgtg gctgcacgcc gccgcggcgc acaccgcgct gaacttcgtc
      841 cgctcgcgcg aacggcgcgg aaagcgtgag ctcgcgcata cacaggagcg cgacgtcgcg
      901 gtcgacccgg cgcgcctcgc cgaggacacc gagacgcgcg cggaggtccg tgccgcgctc
      961 gcccgtctcg acgagcgcag cgcctcgctc ctcgcgctcc gatacagcgg tctgtcctac
     1021 gacgagatcg ccgcggcgct cggcctgcgg cgatcgagca tcggcacctt cctgagccga
     1081 gccgaagcgg cgttccgcaa ggaggtcacc aggtcatgag cgaagacgtc gagaagctcc
     1141 tcgggatcca cgaggtggag gtcgacacgg agcgtgcgct gacgcgcatc cgcgcacgcg
     1201 tcgagcgcga gcgcctgcgt ccggccacat tcgccgtcgc acggcgcgac ggcgcgctcg
     1261 tctccatctg gctgaagggc ctcgccgcgg ccgcctcggt cgcgctcgtc gcggtgctcc
     1321 tgaccgcctc cggcgtcgcc gagacgatcc tcaccatctt cgagccgaag cagatcgtcg
     1381 ccgtgccgat cacgacgacc gacatctcga cagcggcctc cggcctcggc tcgtacggca
     1441 cgctcgagtt ctcgagccag ccgaagccgt acgacgtgcc ggacctcgcg accgcccgaa
     1501 aggacagcgg gctcaccgtc ctcgtgcctg cgcagcttcc cgcgggcgtg agcaccgcgg
     1561 cggcgcgcta cggcgtgatg ccgcggacga ccgcgacctt cacgttcagc gcggcgaaga
     1621 cccaggcagc cgcgtcgcgg ctcggccgca cgcctccgcc catgcccgcg aacatcgacg
     1681 gcagcaagct cttcatcacg ggcggcccgg tggtcgtgca gtacttcggc gacggcacgg
     1741 tgcccacgag cgcgacgggg aatccgttcg ccgggatgcc gcgcctcgtg atcgcgcagg
     1801 gcaagggacc gatcgtgcaa tccgacgggg tgacggtgga gcagctgcag cagtacctgc
     1861 tggcgcagcc gggggtctcg ccgcagctcg cggcgcagat ccgggcgatc aaggacccgt
     1921 cgagcacgct gccgatcccg atcccggttg acatggcgac gtcgaagaac gtgaccgtcc
     1981 agggccagcc gggcgtgttc atcggcgact cgaccgggat gggcagcgcc ctcatctggc
     2041 agaaggacgg catcgtcttc ggcgtggcgg gtacgctcac cgaatcgcag atcttggcgg
     2101 tggcgaactc gctccattga gcgagacccc cgcaatccgc acccggggcc tcgcgaagcg
     2161 ctacggcagc gtccaggcgc tcgcggggct caccatgacc atcccgcgcg gcgcggtctt
     2221 cggcttcctc gggccgaacg gcgccggcaa gacgacggcg gtgaagctgc tgctcggcct
     2281 cacgcggccg aacggcggcg acggcgagat cctcggcctg cccatcggct ccgcggcgtc
     2341 gcgccgccgc gtcggctacc tccccgagct cttccggtac cagggctggc tcaccgcccg
     2401 cgaggtcctc gcgctgcact gcgagctcgc gggcatcgat cgggcggcat ggtcccgcga
     2461 gatcgaggac gcgctcgcga tcgtcggcat cgccgatcgc ggctcgacgc gcgtcggcgg
     2521 gttctcgaaa ggtatgcagc agcgcctcgg ccttggcgtc gcgctgctcg gcgatccgga
     2581 gctcgtgctg ctcgacgagc ccaccagcgc gctcgacccg gtcggccgcc acgacgtccg
     2641 cgagatcatc cgcgccctgc gcgagcgcgg cgtcaccgtc ttcctcaact cgcacctgtt
     2701 gtccgaggtc gagcaggtct gcgaccgcgt cgcgatcgtg gatcgcgggc gcgtcgtcgc
     2761 ggacggcacg ctcgacgacc tcctgcgggg ggacattgtg cgcatccgcg cgaccgagct
     2821 cccgcgcgac gcgctggcgt cgctcgaacg cttcggccgg ctcgacgccg acggcgagtg
     2881 gctcgtcgcg cgcggcatcg ccgaggaccg cgtgcccgag ctcgtcgccg agatcgtacg
     2941 catcggcgga cgc
//
LOCUS       DASTZR010000416         3324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_566925, whole genome shotgun sequence.
ACCESSION   DASTZR010000416 DASTZR010000000
VERSION     DASTZR010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566925"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VFV20_09215"
     CDS             complement(<1..525)
                     /locus_tag="VFV20_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEU5288570.1"
                     /translation="MTASPTVTGTTTAKPKLDISREVGLTPFEAERVRSLMVREPNAL
                     EWAMFGAMWSEHCAYKHSKPLLRTLPTKGPRVAVGPGENAGAVDLGDGLLCVFKIESH
                     NHPSAVEPYQGAATGVCGILRDVFAMGARPVALLNALAFGPPSDPRTRRLVAGIVGGI
                     GSYGNCVGIPDVAGH"
     gene            complement(522..1427)
                     /locus_tag="VFV20_09220"
     CDS             complement(522..1427)
                     /locus_tag="VFV20_09220"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HEU5288571.1"
                     /translation="MSCAGWSARVLRYIDRTEMDLFERQAQLHSLFQRSPVDAAYTQG
                     SASGRRLAGNFSEVDVALLLLDKVKANEFFDYQIYFVGELSKTLETEGLDVVILNQAS
                     LLQRAQVIRSWSILYQRDEKRRVQWETRAVMDYLDFQKYDDLQTKALAERTKSDRFAI
                     DREYVRARLVRLREYTQLLRDLRAIDHVKFKSDPKLYGLAQWYLQQAVELLFGTGAYL
                     VMALALPRPEAYHDILDAVAGHGIIERPLAYRLEHLANLRNLLAYDREQTDIDDVYAQ
                     IQTRLTDLGSFADQVERFVGAGEDA"
     gene            complement(1381..2094)
                     /gene="purQ"
                     /locus_tag="VFV20_09225"
     CDS             complement(1381..2094)
                     /gene="purQ"
                     /locus_tag="VFV20_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEU5288572.1"
                     /translation="MTRVALLRYPGTWSERDFQHAFSLIPDVQTEIVWHEDADLRGFD
                     AAIVPGGFSYGDYLRAGAIAKLSPATKALRGFAAEGKPVLGSCNGFQILCEAGLLPGA
                     LLRNAGLEYRCDWIHVRVENDRTPWTEGLAGAVLRMPIGHGEGCWVADPNTLAAVEEG
                     GQVVFRYTDAQGAATLEANPNGSLANIAGVCNQAGNVVGLMPHPERCVEALLGGTDGY
                     RVLANVVRGLVGTGPALYR"
     gene            complement(2091..2348)
                     /gene="purS"
                     /locus_tag="VFV20_09230"
     CDS             complement(2091..2348)
                     /gene="purS"
                     /locus_tag="VFV20_09230"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017324051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEU5288573.1"
                     /translation="MRFVATIHVRAKPEVRDPQGEAAGGALRALGLDVSNVRTGKEIV
                     VTFEAASEDAAREAVALMGNELLANPVIETYAYELAVGEPA"
     gene            complement(2365..3252)
                     /locus_tag="VFV20_09235"
     CDS             complement(2365..3252)
                     /locus_tag="VFV20_09235"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU5288574.1"
                     /translation="MTTLLQTDVPGATLWRRGKVRDVYEAGGDRLVMVASDRLSAFDV
                     VFPTPIPEKGRILTQLSNFWFARLESLVPNHLVATELVDFPPAFRDVKALAGRSVLVK
                     RCERIDVECVARGYISGSAWTEYKALGTVASEPLPRGLVESARLDEPIFTPATKAVTG
                     HDENISRAQLAALVGTELAKELERVTLALYRAAHAYALGRGLILADTKFEFGFHDGKL
                     TLIDEALTPDSSRYWDAAKYAPGGTPPSYDKQYVRDFVVKSGWNKEPPAPALPPDVVA
                     GTTARYLECYEMLTGTTLR"
BASE COUNT          480 a         1170 c         1143 g          531 t
ORIGIN      
        1 gtggcccgcg acgtcgggga tgccgacgca gttcccgtag gagccgatgc cgccgacgat
       61 ccccgccacc agccggcgcg tccgcggatc ggacggcggc ccgaacgcga gcgcgttcag
      121 gagcgcgacg gggcgcgcgc ccatcgcgaa gacgtcgcgc aggatgccgc acacgccggt
      181 cgccgcaccc tggtacggct cgaccgcgct cgggtggttg tggctctcga tcttgaacac
      241 gcagagcagg ccgtcgccga ggtcgaccgc gccggcgttc tcgcccggac cgaccgcgac
      301 gcgcgggccc ttcgtcggca gcgtgcgaag gagcggcttc gaatgcttgt acgcgcagtg
      361 ctcgctccac atcgcaccga acatcgccca ctcgagcgca ttcggctcgc gcaccatgag
      421 cgagcgcacg cgctccgctt cgaagggtgt gaggccgacc tcgcgagaga tgtccaactt
      481 aggcttcgcc gtcgtcgtcc ccgtcaccgt cggggacgca gtcatgcgtc ctcgccagct
      541 cctacgaagc gctcgacctg atcggcgaac gagccgaggt cggtgagccg cgtctggatc
      601 tgcgcgtaga cgtcgtcgat gtcggtctgc tcgcggtcgt aggcgagcag gttccgcagg
      661 ttcgccaggt gctccaggcg atacgccagc ggccgttcga tgatcccgtg tcccgccacc
      721 gcatcaagta tgtcgtgata cgcctcgggg cgtgggagcg cgagcgccat gaccaggtac
      781 gccccggtgc cgaacaggag ctcgaccgcc tgctgcaggt accactgcgc gaggccgtac
      841 agcttggggt ccgacttgaa cttcacgtgg tcgatcgcgc gcaggtcgcg cagcagctgc
      901 gtgtactcgc gcaggcgcac caggcgcgcg cgcacgtact cacggtcgat ggcgaagcgg
      961 tcgctcttgg tgcgctcggc gagcgccttc gtctggaggt cgtcgtactt ctggaagtcg
     1021 aggtagtcca tgaccgcccg cgtctcccac tgcacgcgcc gcttctcgtc gcgctggtag
     1081 aggatgctcc acgaccggat gacctgggcg cgctgcagca ggctcgcctg gttgaggatc
     1141 acgacgtcca gaccttccgt ctcgagggtc ttcgacagct cgcccacgaa gtagatctgg
     1201 tagtcgaaga attcgttcgc cttcaccttg tccaggagga ggagggcgac gtccacctcg
     1261 ctgaaattgc cagccagacg gcggcctgag gcactgccct gcgtgtaggc cgcgtcgaca
     1321 gggcttcgct ggaacagcga atggagttgg gcctgtcttt cgaagaggtc catctcagtc
     1381 ctatctatat aacgcaggac ccgtgccgac cagcccgcgc acgacattgg ccagcactcg
     1441 atacccgtcc gtgccgccaa ggagtgcctc gacgcaccgt tccgggtgcg gcatgagccc
     1501 cacgacgttg ccggcctggt tgcacacccc ggcgatgttg gccagcgagc cgttggggtt
     1561 cgcctcgagg gtggccgcgc cctgcgcgtc ggtgtagcgg aagacgacct gcccgccctc
     1621 ctcgacggcg gcgagcgtgt ttggatccgc gacccagcag ccctcgccgt gcccgatggg
     1681 catgcgcagg accgcgccgg cgagaccctc cgtccagggc gtgcggtcgt tctcgacgcg
     1741 gacgtggatc cagtcgcagc ggtactcgag gccggcgttc cgcagcagcg cgccgggaag
     1801 gaggcccgcc tcgcacagga tctggaaacc gttgcacgag ccgagcacgg gcttgccctc
     1861 cgcggcgaag ccgcgcaggg ccttcgtggc cggcgagagc ttggcgatcg cgcccgcgcg
     1921 caggtagtcg ccgtagctga agccgcccgg cacgatcgcg gcgtcgaacc cgcgcaggtc
     1981 ggcgtcctcg tgccacacga tctccgtctg cacgtcgggg atgagcgaga acgcgtgctg
     2041 gaagtctcgc tcgctccacg tgccggggta gcggagcagg gcgactcggg tcatgccggc
     2101 tcacccacgg cgagctcgta ggcgtacgtc tcgatcaccg gattcgcgag gagctcgttc
     2161 cccatcagcg ccaccgcctc gcgcgcggcg tcttcgctcg ccgcctcgaa ggtgacgacg
     2221 atctccttgc cggtgcgcac gttcgacacg tcgaggccga gcgcgcgcag cgcgccaccc
     2281 gcggcctcgc cctgcgggtc gcggacctcg ggcttcgcgc ggacgtggat cgtggcgacg
     2341 aacctcatct ttcgcgctcg tctttcaccg cagcgtcgtc ccggtcagca tctcgtagca
     2401 ctcgaggtag cgcgcggtcg tgccggccac gacgtcgggc ggcagcgcgg gcgccggcgg
     2461 ctccttgttc cagccgctct tcacgacgaa gtcgcgcacg tactgcttgt cgtacgacgg
     2521 cggcgtgccg cccggcgcgt acttcgcggc gtcccagtag cgggacgaat ccggcgtgag
     2581 cgcctcgtcg atgagcgtga gcttgccgtc gtggaagccg aactcgaact tcgtgtcggc
     2641 gaggatcagc ccgcgcccga gcgcgtacgc gtgcgccgcg cgatacagcg cgagcgtcac
     2701 gcgctcgagc tccttcgcga gctcggtgcc cacgagcgcg gcgagctgcg cgcgcgagat
     2761 gttctcgtcg tgcccggtca cggccttcgt cgccggcgtg aagatgggct cgtccagacg
     2821 cgccgactcc acgaggccac ggggcagcgg ctcgctcgcc accgtgccga gcgccttgta
     2881 ctcggtccac gccgatcccg agatgtagcc gcgggccacg cactcgacgt cgatgcgctc
     2941 gcaccgtttc accaggaccg accggccggc gagcgccttc acgtcgcgga acgccggcgg
     3001 gaagtccacg agctcggtcg cgacgaggtg attcggcacg aggctctcaa gacgtgcgaa
     3061 ccagaagttc gagagctgcg tgaggatgcg gcccttctcg gggatcggcg tcgggaacac
     3121 gacgtcgaag gcggagagcc gatcgctcgc gaccatgacg agacggtcgc cgccggcctc
     3181 gtacacgtcg cgcaccttgc cgcgccgcca gagcgtcgcg ccggggacgt cggtctgcag
     3241 cagcgtggtc atttctgtcg cgctccgctc cgcccgcttc gcggcgtcgc gtcgccgccg
     3301 cgtcgctccg ctctcgtggt ctcg
//
LOCUS       DASTZR010000417         6994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1041469, whole genome shotgun sequence.
ACCESSION   DASTZR010000417 DASTZR010000000
VERSION     DASTZR010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6994
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041469"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VFV20_09240"
     CDS             complement(<1..677)
                     /locus_tag="VFV20_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288575.1"
                     /translation="MAIPEPVRAAEPVRGSVLNVVPITPPVEATLRILLNSVPFLMGA
                     AVVRQPPGTDGVVASVGRMPWLHDRRLTTLQLPDDTLPANRAVALDWPVPFQAEWVGA
                     PPRLVISRLVFQGRTVGVLLGTLITREPMIQQTREALDLSCELIASAVAGEFAMAIAS
                     PPPMPEGRGATADAPAAPEPEHRPAIASDVVVDEVRRGLAGATDARSLGRVLRDAMSA
                     ITDASAFS"
     gene            complement(802..1578)
                     /locus_tag="VFV20_09245"
     CDS             complement(802..1578)
                     /locus_tag="VFV20_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5288576.1"
                     /translation="MEGTFRLFDLQGKRALVTGASRGIGRAIALTLADAGADVAVTSR
                     NGDDAEPIAREIRAKGRRSVALLLDVRHAQSIRVCFERLDREWRGLDVLVNNAGTNIP
                     QDLFDLDEASWDTVLDADLKGLVFVTQAAAARMAEGGAGGRIVNVASVYGVVGRRERI
                     AYSAAKGAVVNLTRSLALELAPHAITVNAVGPSLVETDLTRERMRTIPGYREEELART
                     PLGRFATPLDVAAAVAFFASPAAAFITGQTLLVDGGTSAH"
     gene            1602..3209
                     /gene="aceB"
                     /locus_tag="VFV20_09250"
     CDS             1602..3209
                     /gene="aceB"
                     /locus_tag="VFV20_09250"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEU5288577.1"
                     /translation="MAVSAAEVRIPKDAADTRGVAEELLTPEAIDFLTRLHREVEPTR
                     LGLLDARQERWKALREGGTLGFLEETAGQRKAEWRVADVPSDLQVRKVEITGPTDRKM
                     IINALNSGASVYMADFEDANTPTWRNIIEGQRNLIDAIERRIEFTNPDGRKYKLNDRT
                     ATLVVRPRGWHLDEKHFLVDVMPVSGAVFDFGLYFFHNAKRLLDKGTAPYFYLPKMES
                     HREARLWSEIFQWAEKELRIPRGSVKATVLVEVITLAFEMDEVLYELRDYSGGLNAGR
                     WDYMFSLIKKFAGRPEFMLPDRAQVTMTVPFMRAYTELLVQTCHRRGALALGGMAAYI
                     PSRRDANVNEIALGKVREDKVREATDGFDGTWVAHPDLVETAMTEFDRVLGHKKNQLD
                     RMRPEVTTTAQQLLDVKIPGSTITDAGLRTNVSVGIQYLASWLRGTGAAAINNLMEDA
                     ATAEISRSQVWQWVHHGAKLAEGPTVTRELVRTVRDEELAKIRTAVGDGAYAQGRFDE
                     AATLFDEVALAPDFVEFLTLPGYTRLE"
     gene            3277..4107
                     /locus_tag="VFV20_09255"
     CDS             3277..4107
                     /locus_tag="VFV20_09255"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU5288578.1"
                     /translation="MTDRVLILVTAASLVLAACGGAARPAAPTADPLAGRYSATGGGG
                     AIAAVNALAKRFAELHPGVVWQIDETGSDAGVNLATTGGVDFGFTSRPLKDDEAKALA
                     SVNIGLAGTGVIVNAGNPVKGLTKDQVKQIFAGQITNWRQVGGDDLDVKVFVREANAA
                     TRSSFESFFFAGKASYAKDATEVYEIDQTLKAVGSFKASIGMATANSKTASDTSVKLL
                     AIDGVAPTPENLVNGSYKVGRPLLIVYPIDASKVRPGITAFLEFVRSPEGQRIAASAN
                     "
     gene            4111..5991
                     /locus_tag="VFV20_09260"
     CDS             4111..5991
                     /locus_tag="VFV20_09260"
                     /inference="COORDINATES: protein motif:HMM:NF026177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5288579.1"
                     /translation="MSLRDAASTLFQPSLRRTLLARSLAISIVPIIIVGMLAFSVSRD
                     LLQARFDDEAEIVASAISTGIEDRITLASRGSSVLAALPEVRDLTANRDAAGLRELLI
                     RLKSRLALDLAFVSDPQGTIIAGAQDFAPGDKLPPELVVRAQAHAEQSYVIYPEARGL
                     MIRSITPISSSTLQNTPGFVEVGSLLDDSFVKTLRASSDSQIAIIVADQVKATTLAGI
                     EESMLPSHTETDLLLGPVRGETTLAGKRYEAILTLLRSHSSEPQELAVFLPLAPLEDA
                     QRQIAAAILVAGAALAALAVFFSYRTAREMTRPLTRLATAARRIESGDLATPVAAGSS
                     HEIGQLERSFGSMAGALQTRDARNDALVNELRAANVKLEEASRLKSEFIANVSHELRT
                     PMNAIIGYTDFLLEGLNGPLTGQQSQDLGRVRAAAANLLGIINGLLDLAKIEAGQMDM
                     QPQRISVGSVAAEVVDLLGERARSKGLALRSDVAPDLRTAWADPYHVRQVLTNLVGNA
                     VKFTAKGEVVIGAEVKDGMIEVVVRDTGEGIAPDAQAYIFDEFRQADGSSRRRHGGTG
                     LGLAIARRLVWMNGGKIWVESEPGIGSRFYFTVPTSSRARALGDGELHPTDTADRLGQ
                     PA"
     gene            6088..6237
                     /locus_tag="VFV20_09265"
     CDS             6088..6237
                     /locus_tag="VFV20_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288580.1"
                     /translation="MGISQILALVALGCGILLLIPTGLGGLPLTAIAIICLALNQSGL
                     LGRIR"
     gene            6282..>6994
                     /locus_tag="VFV20_09270"
     CDS             6282..>6994
                     /locus_tag="VFV20_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oleandomycin glycosyltransferase"
                     /protein_id="HEU5288581.1"
                     /translation="MAHAVFVGVPAHGHVNPTLGIVTELVRRGVRVTYHCGTPYRAAI
                     EAAGATFHAYAADWIDVDMPHDGDVLGLARVIRAAEAKIAPALLPALATERPDVIVHD
                     SLASWGKTLASALRVPAVSSLTTFVFDRRVALARPLLFARFVARHARDRVRLGEMTGL
                     LETITNRERINIVYTSRAFQPFGDRLDASFVFVGPMPRDEPPLDAQLVALQRPIYVSL
                     GTLFNDRAGLFRTLATGLAG"
BASE COUNT         1096 a         2484 c         2391 g         1023 t
ORIGIN      
        1 gagaacgccg acgcgtccgt gatcgcgctc atcgcgtcgc ggaggacgcg gccgaggctg
       61 cgcgcgtcgg tggcgcccgc gagacccctc cggacctcgt cgacgacgac gtcactcgcg
      121 atcgccggac gatgctccgg ctccggcgcc gccggagcgt ccgccgtggc accgcgacct
      181 tccggcatcg gcggcgggga cgcgatggcc atcgcgaact cgcccgcgac ggcggaggcg
      241 atgagctcgc aggagaggtc gagcgcttcg cgggtctgct ggatcatcgg ctcgcgcgtg
      301 atgagcgtgc cgagaagcac gcccaccgtc cgtccctgga acacgagccg cgagatcacg
      361 agccgcggag gcgcaccgac ccactcggcc tggaacggca cgggccagtc gagcgcgacc
      421 gcgcggttcg cgggcagcgt gtcgtccggg agctgcaggg tggtgagccg gcgatcgtgg
      481 agccagggca tgcgtccgac ggacgcgacg acaccatccg tgcccggagg ctgccgcacg
      541 acggctgccc ccatgaggaa cgggacgctg ttcaagagga tgcggagcgt ggcctcgacc
      601 ggaggcgtga tcggcacgac gttcagcacc gatccgcgga cgggctcagc cgcgcgcacg
      661 ggttccggga tcgccaaatg ctcggcctcc tgcgaccgcg cacggtctct cctatttcta
      721 tctggcggaa gcatgacgca gtgaggcgcg gacgggggat gcgtgcctcg ggggagtacg
      781 gacgtacggg ccgggtgagg gtcaatgcgc ggaggtgccg ccgtccacca ggagcgtctg
      841 gccggtgatg aaggcggccg ccggcgaggc gaagaaggcg acggcggccg ccacgtccag
      901 aggcgtggcg aagcggccca ggggcgtgcg cgccagctcc tcctcgcggt agcccgggat
      961 ggtgcgcatg cgctcccgcg tgaggtcggt ctcgacgaga ctcgggccta cggcgttcac
     1021 ggtgatggca tgcggcgcga gctcgagcgc gagggagcgc gtgaggttca ccaccgcccc
     1081 cttggcggcg ctgtaggcga tgcgctcgcg cctgccgacc acgccgtaca cggacgcgac
     1141 gttcacgatg cggccgcccg caccgccctc ggccatgcgc gcggcagcgg cctgggtgac
     1201 gaacacgagc cccttgagat ccgcgtcgag gaccgtgtcc cacgacgcct cgtcgaggtc
     1261 gaagagatcc tgagggatgt tcgtgccggc gttgttcacg agcacgtcga gcccgcgcca
     1321 ctcgcggtcc aggcgttcga agcagacgcg gatggattgc gcatggcgaa catcgaggag
     1381 cagcgcgacg gagcggcgtc ccttggcccg gatctcgcga gcgatcggct cggcgtcgtc
     1441 gccatttcgc gatgtgacag cgacgtcggc accggcgtcg gcgagcgtga gcgcgatcgc
     1501 gcggccgatg ccccgggacg cgccggtgac gagcgctcgc ttcccctgca agtcgaacaa
     1561 ccggaatgtg ccttccatca gggctatgcg agcataggtc gatggccgtg agcgccgcgg
     1621 aagtgcgtat cccgaaggac gcggcggaca cgcgcggtgt cgccgaggag ctcctcaccc
     1681 ccgaggcgat cgacttcctc acgcgactgc atcgcgaggt cgagccgacg cgcctcggac
     1741 tcctcgacgc acgccaggag cgctggaagg cgctgcgcga gggcggcacg ctcgggttcc
     1801 tcgaggagac cgcgggccag cgcaaggccg agtggcgcgt cgcggacgtg ccctccgatc
     1861 tccaggtgcg caaggtcgag atcaccggtc cgacggaccg caagatgatc atcaacgcgc
     1921 tgaactccgg cgcctccgtg tacatggccg acttcgagga cgccaacacg ccgacgtggc
     1981 ggaacatcat cgaaggccag cgcaacctca tcgacgccat cgagcgcagg atcgaattca
     2041 cgaaccccga cgggcgcaag tacaagctga acgaccggac cgcgacgctc gtcgtccggc
     2101 cgcgcggctg gcatctcgac gagaagcatt tcctcgtcga cgtcatgccg gtctcgggcg
     2161 cggtgttcga tttcggcctg tacttcttcc acaacgcgaa gcgccttctc gacaagggca
     2221 ccgcgcccta cttctacctc ccgaagatgg agagccaccg cgaggcgcgc ctctggagcg
     2281 agatcttcca gtgggccgag aaggagctgc gcatcccgcg cggatcggtc aaggcgaccg
     2341 tactcgtcga ggtcatcacg ctcgcgttcg agatggacga ggtgctctac gagctgcgcg
     2401 actactccgg cgggctcaac gccggccggt gggactacat gttcagcctc atcaagaagt
     2461 tcgccggccg tccggagttc atgctccccg accgcgcgca ggtgaccatg accgtgccgt
     2521 tcatgcgcgc gtacaccgag ctgctcgtgc agacctgcca tcgccgcggc gcgctcgcgc
     2581 tcggcggcat ggccgcgtac atcccgagcc ggcgtgacgc gaacgtcaac gagatcgccc
     2641 tggggaaggt gcgcgaggac aaggtgcgcg aggccaccga cggcttcgac ggcacgtggg
     2701 tcgcgcaccc cgacctcgtc gagaccgcca tgaccgagtt cgaccgcgtg ctcgggcaca
     2761 agaagaacca gctcgaccgc atgcgcccgg aggtcaccac cacggcgcag cagctgctcg
     2821 acgtgaagat cccgggctcg acgatcaccg acgcggggct gcgcacgaac gtcagcgtcg
     2881 ggatccagta cctcgcctcc tggctgcgcg gcactggtgc cgcggcgatc aacaacctca
     2941 tggaggacgc cgccacggcc gagatctcgc gctctcaggt gtggcagtgg gtgcaccacg
     3001 gcgcaaagct cgccgagggg ccgacggtca cgcgcgagct cgtccgcacg gtgcgcgacg
     3061 aggagcttgc gaagatccgg accgccgtgg gcgacggcgc ctacgcgcaa ggccggttcg
     3121 acgaggcggc gacgctgttc gacgaggtag ccctcgctcc ggacttcgtc gagttcctca
     3181 ccctccccgg ctacacgcgg ctcgagtagc ccgaacctcc tcccccgttc gggtgtacgg
     3241 cgacgcgggg tgtcgcccca ctatccactc atgcgtgtga ctgatcgcgt cctcatcctg
     3301 gtgacggcgg cgtcgctcgt cctcgcggcc tgcggtggcg cggcgcgacc cgcggcaccg
     3361 accgcggacc ctctcgcggg ccggtactcg gccaccggag gcggcggcgc gatcgccgcg
     3421 gtgaacgcgc tcgcgaagcg attcgccgag ctgcaccccg gggtcgtctg gcagatcgac
     3481 gaaaccggct ccgatgccgg tgtgaatctc gcgacgaccg gcggcgtcga cttcggcttc
     3541 accagccgtc ccctcaagga cgacgaagca aaggcgctcg cctccgtgaa catcggcctc
     3601 gccggcaccg gcgtcatcgt gaacgcgggg aatcccgtga aaggcctcac gaaggaccag
     3661 gtgaagcaga tcttcgccgg acagatcacg aactggcggc aggtcggtgg cgacgacctc
     3721 gacgtgaagg tcttcgtccg cgaggcgaac gccgcgaccc gcagctcctt cgagtcgttc
     3781 ttcttcgccg gcaaggcgag ctacgccaag gacgcgaccg aggtctacga gatcgaccag
     3841 acgctgaagg ccgtcggatc gttcaaggcg tcgatcggca tggcgaccgc gaacagcaag
     3901 accgcatcgg acacgtctgt gaagctgctc gccatcgacg gcgtcgcacc gacaccggag
     3961 aacctcgtga acggcagcta caaggtcggc cgtccgctgc tcatcgtcta tccgatcgac
     4021 gcatcgaagg tgcgccccgg catcacggcg ttcctcgagt tcgtgcgcag ccccgaagga
     4081 caacgcatag ccgcaagcgc gaactgagcg atgtccctcc gcgacgccgc gagcaccctg
     4141 ttccagccga gcctccgccg aacgctcctc gcgcgcagcc tcgcgatcag catcgtgccg
     4201 atcatcatcg tcggcatgct cgcgttcagc gtctcccgcg acctgctcca ggcgcgcttc
     4261 gacgacgagg ccgagatcgt cgcgagcgcg atctccaccg ggatcgagga tcggatcacg
     4321 ctggcttcgc gcggcagctc cgtcctcgct gccctgcccg aggtccgcga cctgacggcg
     4381 aatcgcgacg cggcggggct gcgagagctg ctcattcgcc tcaagtcgcg tctcgccctg
     4441 gatctcgcgt tcgtctcgga tccgcagggc acgatcattg cgggcgctca ggatttcgcg
     4501 ccgggtgaca agctcccgcc cgagctcgtc gtgcgtgccc aggcacacgc agagcagtcc
     4561 tacgtgatct acccggaagc gcgcggcctc atgatccgat cgatcacgcc gatctcctcc
     4621 agcacgctcc agaacacgcc cggcttcgtc gaggtgggct cgctcctcga cgacagcttc
     4681 gtgaagaccc ttcgcgcgtc gtcggactcg cagatcgcga tcatcgtcgc ggatcaggtg
     4741 aaggcgacga cgctggcagg catcgaggag tcgatgctgc cctcgcacac cgagaccgac
     4801 ctcctgctcg ggcccgtccg tggcgagacg acgctcgccg ggaagcgcta tgaagccatc
     4861 ctcacgctgc tgcgttcgca ctcgagcgag ccgcaggagc tcgcggtatt cctgcccctc
     4921 gcgccactcg aggacgcgca gcgtcagatc gccgccgcga tcctcgtcgc gggcgccgcg
     4981 ctcgccgcgc tcgccgtgtt cttctcgtat cgcacggcgc gggagatgac gcgaccgctc
     5041 acgcgcctgg cgaccgccgc gcgccggatc gaatccggtg acctcgccac gcccgtcgcg
     5101 gccggctcgt cgcacgagat cggccagctc gagcgatcct tcggctcgat ggccggcgcg
     5161 ctccagaccc gtgacgcgcg caacgacgcg ctcgtcaacg agctgcgcgc ggcgaacgtg
     5221 aagctcgagg aggcgagccg cctgaagtcc gagttcatcg ccaacgtcag ccacgagcta
     5281 cgcacgccga tgaatgcgat catcggctac accgacttcc tgctcgaggg tctgaacggg
     5341 ccgctcaccg gccagcagtc gcaggacctc ggacgtgtgc gcgccgccgc cgcgaacctg
     5401 ctcggcatca tcaacggcct gctggatctc gcaaagatcg aggcaggtca gatggacatg
     5461 cagccacagc ggatcagcgt gggctcggtc gcggcggagg tcgtcgatct cctcggcgag
     5521 cgcgcgcggt cgaagggcct tgcgctccgt tcggacgtcg cacccgacct gcgcaccgcg
     5581 tgggccgatc cgtatcacgt gcggcaggtg ctcacgaacc tcgtcggcaa cgcggtcaag
     5641 ttcaccgcga agggcgaggt cgtcatcggc gcggaggtga aagatgggat gatcgaggtc
     5701 gtcgtccgtg acaccggcga gggcatcgcc cccgacgcgc aggcctacat cttcgacgag
     5761 ttccgccagg cggacgggtc ctcgcgccgc cgccatggcg gcaccggcct cgggctcgcc
     5821 atcgcgcgcc gcctggtgtg gatgaacggc ggcaagatct gggtggagag cgagccgggc
     5881 atcggttcac gcttttactt caccgtgccc acgagctcgc gcgcgcgcgc ccttggcgac
     5941 ggcgagctgc acccgacaga caccgcggat cggctgggtc agccggcgta gctcgcgtct
     6001 ccgacctcgc gggcgccacg cggacgggcc cgccggacgg catgctgggt gcagggggac
     6061 cgagcgagct ttcgcatagg aggtcacatg ggaatcagcc agatcctcgc tctcgtcgcg
     6121 ctcggctgcg gcatcctcct cttgatcccg accggactcg gcggcctacc gctcacggcg
     6181 atcgcgatca tctgcctcgc gctcaaccag tccggccttc tcggtcgcat ccgctgagct
     6241 tcgcccgcta gccctctcca cgcgcggggt agcgtggtcg ggtggcgcac gccgtcttcg
     6301 tgggcgtccc ggcgcacggg catgtcaatc ccacgctggg gatcgtcacc gagctcgtgc
     6361 gccgcggcgt ccgcgtcacg taccactgcg gcacgccgta ccgggccgcc atcgaagcgg
     6421 ccggtgcgac gttccacgcg tacgccgcgg actggatcga cgtggacatg ccgcacgacg
     6481 gcgacgtgct cggcctcgcc cgtgtgatcc gcgccgccga ggcgaagatc gcgccggcgc
     6541 tcctgccggc gctcgcgacc gagcggcccg acgtgatcgt gcatgactcg ctcgcgtcgt
     6601 ggggcaagac gctcgcgtcc gcgctgcggg tcccggccgt ctcctcgctg acgacgttcg
     6661 tgttcgaccg ccgcgtggcg ctcgcgcgcc cgctgctctt cgcgcgcttc gtcgcccgcc
     6721 acgcgcgcga ccgcgtccga ctcggcgaga tgacggggct gctcgagacg atcacgaacc
     6781 gcgagcgcat caacatcgtc tacacgtcgc gcgcgttcca gccgttcggc gatcggctcg
     6841 acgcgagctt cgtcttcgtc ggcccgatgc cgcgtgacga gccgccgctc gacgcgcagc
     6901 tggtggccct gcagcgtccg atctacgtgt cgctcggaac gctcttcaac gaccgggccg
     6961 ggctcttccg gaccctggcc acggggctcg cggg
//
LOCUS       DASTZR010000418         2332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1296612, whole genome shotgun sequence.
ACCESSION   DASTZR010000418 DASTZR010000000
VERSION     DASTZR010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296612"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VFV20_09275"
     CDS             complement(<1..236)
                     /locus_tag="VFV20_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288582.1"
                     /translation="MSSPRAPAGIAPRLFLIASAIVAITALVVAFGDTAAKRPAPEPA
                     TMSPFPTGEPPLTAEADVFRTHAGVLAIDPGSEPR"
     gene            complement(233..>2332)
                     /locus_tag="VFV20_09280"
     CDS             complement(233..>2332)
                     /locus_tag="VFV20_09280"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU5288583.1"
                     /translation="KGLACAKGYYSVQALYGRDRITRARIRRDSGLADAPMSEALDLV
                     AQRLRETIQQHGKDSVALYGSAQWAIPDAYVASKLFKGGIGTNNVESSSRLYAASTMA
                     ALETSFGLDGSIGSYEDVDQADVFILWDINLAETDPVLFSRMLDRRRRDRSVRIVDLT
                     TRTTRTSYAADRSLLHTPHSGLAIANAIAREIVARKWVNREFVERHVAFKRGKTGIGY
                     GLTDDSLVADGASEASLDEYESFLAPYTAERAQQLSGLGADDVRWLATLYGDRARKVM
                     TVWGAEANRHSRGTWQNNLLYNIHLLVGKIASPGNAALSLTGEPNGGSSVHDAGTLTH
                     TLPRGVVASAADRRRAAAIWGVAEDAIAPRPTRTALPLFRALDRGDVRFLWIQATNPM
                     VSLPHLARYRRAAAKEGRFIVVSEAYPTPTTDVADVVLPAAMWLEREGVFANVERRIQ
                     HFEQLVAPPDEAMSDAWQMIEVARRMGMSRLFPWDRRDHVARAWEEYRRFHDQPRSAL
                     PALSELRARPGVVWPVAGGRETKWRYNTAHDAAADRARGGFDFYGHADHKAWIWLRPH
                     EPPAESPDRAFPFWLTVGAVLEHSGAGAMTRRIPTLHRSVPRAYLEMNREDARRLGIA
                     NREMVRLVSRRGSVEIEARIDYRSRPPRGQLFVPSFDEAVPVGQLMLDAFCPLSGQPD
                     TTICAVRVERLGGRNGS"
BASE COUNT          342 a          806 c          812 g          372 t
ORIGIN      
        1 cgcggctccg atcccggatc gatcgccagc accccggcat gagtccggaa cacgtccgcc
       61 tcggcagtga gcggaggctc gcccgtgggg aatggcgaca tcgtggcagg ctccggtgcg
      121 ggccttttcg cggcggtgtc gccgaacgcg acgaccagcg ccgtgatggc gacgatcgcc
      181 gacgcgatca ggaaaagccg cggcgcgatt ccggcgggcg cccgcgggga gctcacgatc
      241 cattccttcc gccgagtcgc tcgacgcgaa ccgcacagat ggtggtgtcg ggctggcccg
      301 acagcggaca gaacgcgtcg agcatgagct gcccgacggg gaccgcctcg tcgaacgatg
      361 gcacgaacag ctgcccgcgc ggtggtcgcg agcgataatc gatgcgcgct tcgatctcca
      421 ccgagccgcg ccggctcacc agtcggacca tctcgcgatt ggcgatgccc agtcgtctgg
      481 cgtcctcgcg attcatctcg aggtacgcgc gtggcacgga ccggtgcagg gtggggatcc
      541 gccgcgtcat cgcgccggcg cccgaatgct cgagcaccgc gcccacggtg agccagaatg
      601 gaaacgctcg gtcgggcgat tccgccggcg gctcgtgcgg acggagccag atccacgcct
      661 tgtgatcggc gtgcccgtag aagtcgaagc cgccgcgcgc gcggtcggcc gcggcatcgt
      721 gcgcggtgtt gtaccgccac ttggtctcgc ggcctccggc gaccggccac acgactccgg
      781 gacgcgcgcg cagctcggag agcgcgggca gcgcgctgcg gggctggtcg tgaaaccgtc
      841 ggtactcctc ccaggcccgc gcgacgtgat cgcgccggtc ccatggaaag agtcgcgaca
      901 tccccatgcg cctggcgact tcgatcatct gccatgcgtc gctcatcgct tcatcgggcg
      961 gcgcgacgag ctgctcgaag tgctggatcc gccgctcgac gttggcgaac acgccttcgc
     1021 gctcgagcca catcgccgcc ggcagaacga cgtccgccac gtccgtggtc ggcgtcggat
     1081 atgcttcgga tacgacgatg aagcgtcctt ccttcgcggc cgcgcggcga taccgcgcga
     1141 ggtggggcag gctcaccatc gggttcgtcg cctggatcca caggaagcgt acgtcgccgc
     1201 ggtccagggc tcggaacagg ggcagcgcgg tgcgcgtcgg ccgcggcgcg atcgcatcct
     1261 cggccacgcc ccagatagcg gctgcccggc gacgatcggc ggcgctcgcc accactccgc
     1321 gcggaagcgt gtgcgtcagg gtccccgcgt cgtgcaccga gcttccaccg ttcggctccc
     1381 cggtgagcga cagcgcggcg ttgcccggcg acgcaatctt gccgacgaga agatggatgt
     1441 tgtagagcag gttgttctgc cacgtgccgc gcgaatgccg gttcgcttcg gcgccccaca
     1501 cggtcatgac cttgcgcgcg cggtctccgt acagcgtcgc cagccagcgc acgtcgtccg
     1561 cgcccagtcc ggagagctgc tgcgcgcgct ccgcggtgta cggcgcaagg aacgactcgt
     1621 actcgtccag gctcgcctcg ctcgctccgt ccgcgaccag ggagtcatcg gtgaggccgt
     1681 agccgattcc ggtcttcccc cgcttgaagg cgacgtgccg ctctacgaac tcccggttga
     1741 cccatttgcg cgcgacgatc tcgcgcgcga tggcgttcgc gattgcgagt cccgagtgcg
     1801 gcgtgtgcag gagggaccga tccgcggcgt agctcgtgcg cgtggtgcgc gtggtgaggt
     1861 ccacgatccg caccgagcgg tcgcggcgcc tgcggtcgag catgcgcgag aagaggaccg
     1921 gatccgtctc ggcgagattg atgtcccaaa ggatgaagac gtcggcctgg tcgacgtctt
     1981 cataggatcc gatcgagccg tcgaggccga agctcgtctc gagggcggcc atggtactgg
     2041 ccgcgtacag ccgggagctc gactcgacgt tgttggtgcc gattccgccc ttgaagagct
     2101 tggacgccac gtatgcgtcc gggatggccc actgcgccga gccgtacagc gcgacgctgt
     2161 cctttccgtg ctgctgaatc gtctcgcgca gccgctgcgc cacgaggtcg agcgcctcgc
     2221 tcatcggcgc gtcagcgagc ccgctgtcgc gccggatcct ggcgcgggta atgcggtccc
     2281 ggccatacaa ggcctggacc gagtagtagc ctttcgcgca agcgagccct tt
//
LOCUS       DASTZR010000419         7824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_43741, whole genome shotgun sequence.
ACCESSION   DASTZR010000419 DASTZR010000000
VERSION     DASTZR010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7824
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43741"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..1563)
                     /locus_tag="VFV20_09285"
     CDS             complement(172..1563)
                     /locus_tag="VFV20_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288584.1"
                     /translation="MGAFFFPVAQRALLAVVAVLVVVAPMPGVHYREAADAAPGTATG
                     ARVSPADLRDPDAPQIQRFADVSAGVSITPPAGWVRSPDSALNPVSDPVEPVQEIARF
                     QIRVGDAQLYAAPIAITSGLVADAIALISIGVAREGSDILLAERRARLDRELGSIPHF
                     TTREDEMTYEDLHVLTRYLFSRESDRALVVRAAAAESSWPTFEPLMRAAIATFTGDPL
                     AANAPAPAPPAPPPAPEPVEATDPTVEIRNQILARAASLIGLRYVWGGNSIVAGMDCS
                     AYVSWAWGVSRYTTDSIWQVSFAIAKDDLRPGDAVNLTIGRDPARRGHIRLFEAWANA
                     EHSAMWVYEETPPRVVHRVVAYDGRYQPIRLAGLSGAGEARLVPGAPAPEPTAAPVFR
                     RTPAPTYRATPRPTVRPTLRPTPRPTVRVTPRPTATIRAATTATPVPTRTTNGTFAPM
                     PSPTRTPAPTRRP"
     gene            complement(1621..2355)
                     /gene="radC"
                     /locus_tag="VFV20_09290"
     CDS             complement(1621..2355)
                     /gene="radC"
                     /locus_tag="VFV20_09290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEU5288585.1"
                     /translation="MSTAAAESGFPRLRQARSDHVRVPIHEWPESVRPRERLAARGAQ
                     ALSDDELIALLIGSGTRGRSALVMANELLREVGGLRALGQADVARLRARLGPATAVRI
                     VAACELGRRVVIAGADGERCDTPELAAVALAPHFAHLEREALVVALLTRKQRLASVTP
                     LYRGSVSGTTVRIAELFEDAIRRGMPAILLAHNHPSGDPTPSADDIRTTRDAAQAGRL
                     LGIEVVDHLVFGAGRWVSLRRAGALA"
     gene            complement(2442..2972)
                     /locus_tag="VFV20_09295"
     CDS             complement(2442..2972)
                     /locus_tag="VFV20_09295"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEU5288586.1"
                     /translation="MPDLGGLWTGFVNNPLVQLAARGVSLYVLFLYLATVFWTVRDAQ
                     QRTENRITPYLAGLMVVATWFLGLIIYLIVRPRETLGEAYERQLAEESLLAEAEQRLV
                     CPTCRERVQEEYILCPTCRTRLKRQCPSCGKLIRPEWNICPYCTKDFDERDWTVHAGG
                     KAAASAGSRDATEKLP"
     gene            complement(3062..4126)
                     /locus_tag="VFV20_09300"
     CDS             complement(3062..4126)
                     /locus_tag="VFV20_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEU5288587.1"
                     /translation="MRVLITGITGMAGSHLAEYLLAEHPQVAVYGTFRWRSRMENLEP
                     LRRARKVDVVEGRFTDGQALRGEDKKGRVTLLYSDLTDAGSVEHLITSVQPERIFHLA
                     AQSFVQESFDEPSSTFHINVQSQINLLESIRRHDKQIRMHVAGSSEEYGLVHPDEVPM
                     KETNPLRPLSPYAVSKVAQDKLAYQYFKSYGLHLVVTRGFNHTGPRRGPVFADSTFAK
                     QIAEIEAGILPAVIRHGDLTTKRDLSDVRDIVRAYWLALEKAEPGEAYNVGSGKTHTI
                     REMLDVLLSYAKVKIRTEEDPARLRPSDVPILWADPSKFRAATGWEPKIPFEQTLRDV
                     LDYWRERVRAEGGQQARAEV"
     gene            complement(4218..4628)
                     /locus_tag="VFV20_09305"
     CDS             complement(4218..4628)
                     /locus_tag="VFV20_09305"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEU5288588.1"
                     /translation="MRTIRQTALIRGATPHDIFETLMDAKRHSTLVGGQRVKVSRRPG
                     GAFSVGHDLEGRTLRVAKDGRIVQSWRANNWPKGLYSKATFALAKTPGGTRITFTQTG
                     VPSAFYGEIASGWRTYYWTPLRKQFASRRSGEAA"
     gene            4660..5190
                     /locus_tag="VFV20_09310"
     CDS             4660..5190
                     /locus_tag="VFV20_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU5288589.1"
                     /translation="MPVLDISRHAQRRSLDDQQSALSTAGAQMCERLAKTAPKYALVV
                     SSPLPRARETAERIAGRLDAVEPGLLPDLGSHAMSLFGELRTLADWARLLRDEPQART
                     FAEEQLPTWSRLASRVAEKERVLAVSHGGIIELPAILLTQRLGAKVDGASFGYGEGVR
                     VTYAEGKATAIEILRG"
     gene            complement(5216..6643)
                     /locus_tag="VFV20_09315"
     CDS             complement(5216..6643)
                     /locus_tag="VFV20_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEU5288590.1"
                     /translation="MPDPIEERPQVVAALRMAMAEAERYLRGIDADHARRADALAVAE
                     SIGGELPEQGIGALAAIAELARASDAATRSSGPRFFHWVTGGGTPAALAADWLTSVYD
                     NNSFAWESSPLGTRLEELSIAWLKDLFGLPGAWGGVLTTGATMANFTALAAARRWWGE
                     RHGADVDREGLGGLPAVPVFSSGYIHVSATKALAMLGIGRETVRRLSRDDVGRLDVRA
                     LEDALKALGGAPAIVVANAGEVNAGDFDPIERMAELCDRYGAWLHVDGAFGLFAAVSP
                     RTRPLVAGIGRAHSAISDGHKWLNVPYDSGFAFVRDPLLLAHAFAFTAPYLPGPGQHP
                     NFGMLAPEASRRARAIAVWATLRAYGREGHRAMVERHLDLAQHLARRIDEAADFERVA
                     EVPLNIVAFRYRPAGLAAERLDDLNARIGTEVLTDGRIYMGTTRYRGMTVFRPAIVNW
                     RTTEADVDLIIDVVRELGERLASGS"
     gene            complement(6654..7265)
                     /gene="purN"
                     /locus_tag="VFV20_09320"
     CDS             complement(6654..7265)
                     /gene="purN"
                     /locus_tag="VFV20_09320"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEU5288591.1"
                     /translation="MTFPIGVLVSGRGTNLQALLDACASGTLDAQVVFVASNRENVPA
                     LIRAERAGVPHAAFTVAEYGSRAAAQGAIADALASAGTRLVVLAGFDHILDPAFFVKL
                     AGVPVINIHPALLPFFGGKGMHGDKVHAAVLAAKAKESGVTVHRVHPDTVDLGEVIVQ
                     RRVPVHEGDDVPALSNRVLAVEHEAIVEAVRRCIAHDLTGRSA"
     gene            complement(7262..>7824)
                     /locus_tag="VFV20_09325"
     CDS             complement(7262..>7824)
                     /locus_tag="VFV20_09325"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEU5288592.1"
                     /translation="DAILDPKNVRTGDVCVGLPSSGLHTNGYTLARAIAARSASAQGK
                     DVLAVLTAPRADLDGASLGDALLRTHTSYLAAFRTLRDAATIRAIAHLTGGGWEGNLP
                     RALPAGLGAAIDRGTWRVPAVFRVLADLGAVPEDDQWDTWNMGIGLVAIVSAETADAA
                     LRALPDAVRLGTIERAREGERRIRFT"
BASE COUNT         1144 a         2710 c         2846 g         1124 t
ORIGIN      
        1 acacgagcgt gttcgcggtg ccgaggtcga tgccgatgtc gcgtgagaag aggccgaaaa
       61 ggccatcgaa cacgagcgga tatttccctt ccaaatgcgc agccggatcg ctcgaagcat
      121 aacggcgcaa gagcgtgaaa cgcgcgctgg acacgcgcgc gaggcgcggg gctagggccg
      181 ccgtgtgggt gccggcgtcc gtgtggggct cggcatggga gcgaacgttc cgttggtggt
      241 ccgggtcggc accggagtgg ccgtcgtcgc ggcgcggatc gtcgccgtcg ggcgtggcgt
      301 gacgcggacc gtcggtcgcg gcgtcggccg gagtgtgggc cggaccgtcg ggcgcggggt
      361 cgcccggtac gtgggggcgg gcgtgcgccg gaacacgggc gcggcggtgg gctcgggcgc
      421 gggagcgccg ggcaccaggc gtgcctcacc ggcgccggaa aggcccgcaa gccttatcgg
      481 ctggtagcgg ccgtcgtacg caacgacccg gtggacgacc cgcggcgggg tctcttcgta
      541 gacccacatc gcgctgtgct ccgcgttcgc ccatgcctcg aacagacgga tgtgccccct
      601 ccgggcgggg tcacggccga tggtcaggtt cacggcgtcg cccggccgga ggtcgtcctt
      661 ggcgatggcg aacgacacct gccagatgga gtcggtggtg tagcgcgaca cgccccaggc
      721 ccagctgacg taggcagagc agtccatgcc ggcgacgatc gagttcccgc cccagacgta
      781 gcgcagcccg atcaggctcg cggcacgagc gaggatctgg ttccgaatct cgacggtggg
      841 atcggtcgcc tcgacgggct cgggcgccgg gggcggtgcc ggcggggcgg gcgcgggtgc
      901 gttcgcggcg agcgggtcac cggtgaacgt agcgatcgcg gcgcgcatca ggggctcgaa
      961 ggtcggccag ctgctctcgg ccgcggccgc gcgcacgacg agcgcgcgat ccgactcgcg
     1021 ggagaagagg taccgcgtca ggacgtggag gtcctcgtag gtcatctcgt cctcgcgggt
     1081 cgtgaagtgc gggatgctgc cgagctcacg gtccaggcgc gccctgcgct ccgccaggag
     1141 gatgtcgctg ccctcgcggg caacaccgat cgagatgagc gcgatcgcgt cggccaccag
     1201 tccgctcgtg atcgcgatcg gtgcggcgta gagctgggcg tcgccgacgc ggatctggaa
     1261 gcgcgcgatc tcctgcaccg gctcgaccgg gtcggagacc gggttgagcg cgctgtcggg
     1321 gctgcggacc cagccggcgg gcggcgtgat ggagacgccc gcgctgacgt cggcgaaccg
     1381 ctggatctgt ggcgcgtcgg ggtcacggag gtcggccggc gacacgcgcg cgccggtcgc
     1441 ggtgccgggc gcggcgtcgg ccgcttcgcg gtagtggacg cccggcatcg gggcgacgac
     1501 gacgaggacc gccacgacgg cgagcaacgc acgctgcgcg acagggaaga agaatgctcc
     1561 caccgaggct ggcaacgagc caagggacga cctggtacag cctgctacgt ggttgacacg
     1621 ctacgcgagg gcgccggcgc ggcgcaggct cacccagcgg cccgcgccga acacgagatg
     1681 gtcgacgacc tcgatgccca ggaggcgccc agcttgcgcc gcgtcgcgag tggtgcggat
     1741 gtcgtcggcc gacggcgtgg gatcgccgct cggatggttg tgcgcgagga ggatcgcggg
     1801 catcccccgc cggatggcgt cctcgaacag ctcggcgatg cggacggtcg tcccggacac
     1861 gctgccccta tacaaaggag tgaccgacgc gaggcgttgc ttgcgcgtga gcagcgcgac
     1921 gacgagcgcc tcgcgctcca gatgggcgaa gtgcggcgcg agcgcgaccg cggcgagctc
     1981 cggggtgtcg cagcgctcgc cgtcggcgcc ggcgatgacg acccggcgtc ccagctcgca
     2041 cgccgcgacg atgcggaccg cggtcgcggg gccgaggcgc gcgcgaagac gcgcgacgtc
     2101 ggcctgcccg agcgcgcgca ggccgcccac ctcgcggagg agctcgttcg ccatgaccag
     2161 ggccgacctg ccgcgcgtcc cgctgccgat gagcaatgcg atcaactcgt catcggacag
     2221 cgcttgcgca ccgcgggccg cgagccgctc gcgtggtcgc acggactcgg gccactcgtg
     2281 gatcgggacg cgaacgtgat cggaccgtgc ttgacgcagg cgcggaaatc cggactccgc
     2341 cgcagccgtg gacatggtga tcgagccggt ctccgccggc tacccctgcg gcgaggtcag
     2401 cagcaagccg cggatcgaag tggcggagcg ggccgcggtc gctagggcag cttctccgtc
     2461 gcgtcgcgcg atcccgcgct cgcggccgcc ttgccgccgg cgtgcacggt ccagtcgcgc
     2521 tcgtcgaagt ccttcgtgca gtacgggcag atgttccact cgggccggat cagcttcccg
     2581 cacgaggggc actgccgctt gagacgggtg cggcacgtgg gacagaggat gtactcctcc
     2641 tgcacgcgct cgcggcacgt cgggcacacg aggcgctgct cggcctcggc gaggaggctc
     2701 tcctcggcga gctgccgttc gtacgcctcg ccgagcgtct cacgcgggcg aacgatgagg
     2761 tagatgatca gacccaggaa ccacgtcgcc acgaccatga ggcccgcgag gtacggcgtg
     2821 atgcggttct cggtccgctg ctgcgcgtcg cgcaccgtcc agaacaccgt cgcgaggtac
     2881 aggaacagga cgtacaggct cacgccgcgg gcggcgagct gcaccagcgg gttgttgacg
     2941 aatccggtcc acagtccacc gagatcgggc atctaattga gtctccggct gcgccgggag
     3001 aaacgccccg gcgggctgac tcgctcgtcg cggatgaacc gctcctcgct cgtcttcatg
     3061 actaaacctc cgcgcgcgct tgctggccgc cttcggccct cacgcgctct ctccagtaat
     3121 caaggacgtc gcgtagcgtc tgctcgaacg ggatcttcgg ctcccagccg gtcgcggcgc
     3181 ggaacttcga tgggtcggcc cacaggatcg gcacgtcgct cggccgaagg cgcgccggat
     3241 cctcctcggt gcggatcttc accttggcgt acgagaggag aacgtcgagc atctcgcgga
     3301 tcgtgtgcgt cttcccgctg ccgacgttgt acgcctcacc cggctcggcc ttctcgagcg
     3361 cgagccagta cgcgcgcacg atgtcgcgca cgtcggagag gtcgcgcttc gtcgtgaggt
     3421 cgccgtgcct gatcaccgcc ggcaggatgc ccgcctcgat ctcggcgatc tgcttcgcga
     3481 aggtgctgtc ggcgaacacc ggtccgcggc gcgggccggt gtggttgaag ccgcgcgtca
     3541 ccacgaggtg cagcccgtac gacttgaagt actggtaggc gagcttgtcc tgcgcgacct
     3601 tgctcaccgc gtacggcgag agcggacgca gcgggttcgt ctccttcatc gggacctcgt
     3661 ccggatgcac gaggccgtac tcctcgctcg agccggcgac gtgcatgcgg atctgcttgt
     3721 cgtggcggcg gatcgactcg aggagattga tctgcgattg gacgttgatg tggaacgtcg
     3781 acgacggctc gtcgaagctc tcctgcacga acgactgcgc cgcgagatgg aagatgcgct
     3841 ccggctggac cgacgtgatg agatgttcca ccgatcccgc gtcggtgagg tcgctgtaca
     3901 ggagcgtgac gcgccccttc ttgtcctcgc cgcgcaacgc ctgaccgtcg gtgaagcggc
     3961 cctcgaccac gtcgaccttg cgcgcgcgcc ggagcggctc gaggttctcc atgcgcgagc
     4021 gccagcggaa cgtgccgtat acggcgacct gcgggtgctc ggcaaggagg tactcggcga
     4081 ggtgacttcc ggccatgccg gtgatgccgg tgatcaggac gcgcattcgg caccgagcct
     4141 agcaaaaggt atgccgtgcc cggtcgctga gcggtctagc gtgccttcac gaaggccttg
     4201 cgcagaggcg tccagtacta cgcggcttcg ccgctccgtc tggacgcgaa ctgcttgcgc
     4261 agaggcgtcc agtagtacgt acgccagccg ctcgcgatct cgccgtagaa cgcgctcggc
     4321 acgcccgtct gcgtgaaggt gatgcgtgta ccgccgggcg tcttcgcgag cgcgaacgtc
     4381 gccttcgagt acaggccctt cggccagttg ttcgcacgcc aggactggac gatgcgcccg
     4441 tccttcgcca cccggagcgt ccttccctcg aggtcgtgac caacgctgaa cgcaccgcct
     4501 gggcgccggc tcactttcac ccgctgcccg ccgacgagcg tgctgtgtcg tttcgcgtcc
     4561 atcagcgtct cgaagatgtc gtggggcgtc gcgccccgga tgagcgcggt ctgccggatc
     4621 gtacgcatgt ccttccctcc agacgggcag actagcggca tgccggtcct cgacatcagc
     4681 cgtcatgcgc agcgtcgatc actcgacgat cagcagtcgg cgctttcgac ggcgggcgcg
     4741 cagatgtgcg agcgcctcgc gaagacggcg ccaaaatacg ccctggtcgt ctccagcccg
     4801 ctgccgcgtg cgcgggagac cgcggaacgc atcgccgggc ggctcgacgc cgtcgagccg
     4861 ggtctgctgc cggatctcgg cagccacgcc atgtcgctgt tcggcgagct gcgcacgctc
     4921 gccgactggg cacgcctcct gcgcgacgag ccgcaggcgc ggacgttcgc cgaggagcag
     4981 ctcccgacgt ggtcgcgcct cgcgtcgcgc gtcgccgaga aggagcgcgt cctcgcggtg
     5041 tcccatggcg ggatcatcga gctgcccgcg atccttctca ctcagcggct gggcgcgaag
     5101 gtcgacggcg cgtcgttcgg ctacggcgag ggcgtgcgtg tcacatacgc ggaggggaag
     5161 gcgacggcga tcgagatcct ccgcgggtag tcgccggctt caggagcgtc gaacatcatg
     5221 acccagaggc aaggcgctcg ccgagctccc gaacgacgtc gatgatgaga tccacgtccg
     5281 cctccgtcgt ccgccagttc acgatcgccg ggcggaacac ggtcatcccc cgataccgcg
     5341 tcgtccccat gtagatgcgg ccgtcggtga gcacctcggt cccgatccgc gcattgaggt
     5401 cgtcgagccg ctcggccgcg aggcccgccg gacggtagcg gaacgcgacg atgttgagcg
     5461 gcacctccgc cacgcgctcg aagtccgcgg cctcgtcgat gcggcgcgcg aggtgctgcg
     5521 cgaggtcgag atgccgctcg accatcgcgc gatgaccctc acggccgtac gcgcgcaggg
     5581 tcgcccacac cgcgatggca cgcgcgcgcc gcgacgcctc gggcgcgagc atgccgaagt
     5641 tcggatgctg tcccgggccc ggcaggtacg gcgccgtgaa cgcgaacgcg tgcgcgagga
     5701 gcagcgggtc gcgcacgaac gcgaagcccg agtcgtacgg cacgttgagc cacttgtgcc
     5761 cgtcgctgat cgccgagtgc gcgcgtccga tccccgcgac gagcggacga gtgcgcggcg
     5821 agaccgccgc gaacagaccg aaggcaccgt cgacgtggag ccacgcgcca taccgatcgc
     5881 agagctcggc catgcgctcg atgggatcga agtcgccggc gttcacctcg cccgcgttcg
     5941 cgacgacgat cgccggcgca ccaccgagcg ccttgagcgc atcctcgagc gcgcgcacgt
     6001 caagacggcc gacgtcgtcg cgtgacaggc ggcgcaccgt ctcgcggccg atgccgagca
     6061 tcgcgagcgc cttcgtcgcg ctcacgtgga tgtagccgct cgagaacacc gggacggcgg
     6121 gaaggccgcc gaggccctcg cggtcgacgt ccgctccgtg ccgttcgccc caccagcgcc
     6181 gcgccgccgc gagcgcggtg aagttcgcca tcgtcgcgcc ggtggtgagg acgccgcccc
     6241 atgcgccggg caggccgaag agatctttca gccacgcgat cgaaagctcc tcgaggcgcg
     6301 tcccgagggg cgagctctcc caggcaaagg agttgttgtc gtacacggac gtgagccagt
     6361 ccgccgcgag cgcggccggc gtcccgccgc cggtcaccca gtggaagaag cgcggtccgc
     6421 tcgatcgcgt cgcggcatcg ctcgcgcgcg ctagctcggc gatcgccgcg agcgcgccga
     6481 tcccctgttc gggcagctcg cccccgatcg actcggccac cgcgagcgca tcggcccggc
     6541 gagcgtgatc ggcgtcgatc ccgcggagat agcgctccgc ctcggccatc gccatgcgca
     6601 gcgcggcgac gacctgcggg cgttcctcga tcgggtcggg cacgcgcgag atgctaggcc
     6661 gagcgtcccg tcaggtcgtg cgcgatacag cgccggacgg cctcgacgat cgcctcgtgc
     6721 tccacggcga gcacgcgatt cgaaagggcc gggacgtcgt cgccctcgtg caccggcacg
     6781 cgccgctgca cgatgacctc gccgagatcg acggtgtcgg gatgcacccg gtgcaccgtg
     6841 acacccgact ccttcgcctt cgcggcgagc acggcggcgt gcaccttgtc accgtgcatg
     6901 cccttgccgc cgaagaacgg cagcagcgcc gggtggatgt tgatgaccgg cacgcccgcc
     6961 agcttcacga agaacgcggg atcgaggatg tggtcgaacc ccgcgagcac gacgaggcgc
     7021 gtgcccgccg atgcgagcgc gtccgcgatc gcgccctgcg ccgcggcgcg ggagccgtac
     7081 tcggcgaccg tgaacgcggc gtgcggcacg ccagcgcgct ccgcgcggat cagcgccggg
     7141 acgttctcgc gattcgacgc gacgaagacg acctgcgcgt cgagcgtgcc ggacgcgcac
     7201 gcgtcgagga gcgcctgaag gttcgtgcct cggcccgaga cgagcacgcc gatcgggaag
     7261 gtcatgtgaa gcggatcctc cgttctcctt cgcgtgcgcg ctcgatcgtg ccgaggcgca
     7321 cggcgtcggg cagcgcgcgc agcgcggcgt ccgccgtctc cgcggacacg atggccacga
     7381 gaccgatgcc catgttccag gtatcccact gatcgtcctc agggaccgcg cccagatccg
     7441 cgagcacgcg gaagaccgcg ggcacccgcc aggtcccacg gtcgatggcc gccccaaggc
     7501 ccgcgggcag cgcgcgcggg aggttgccct cccatccgcc gccggtgagg tgcgcgatcg
     7561 cgcggatggt cgcggcgtcg cgcagcgtgc ggaacgccgc gaggtaggac gtgtgggtcc
     7621 gcaggagcgc gtcaccgagg gacgcgccgt cgaggtccgc gcgcggcgcg gtgagcaccg
     7681 cgagcacgtc cttcccctgg gccgacgcgc tgcgtgcggc gatcgcgcgc gcgagcgtat
     7741 agccgttggt gtgcaggccg ctcgagggga gcccgacgca cacgtcgccg gtccgcacgt
     7801 tcttcgggtc gaggatcgcg tcgc
//
LOCUS       DASTZR010000420         2326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_567184, whole genome shotgun sequence.
ACCESSION   DASTZR010000420 DASTZR010000000
VERSION     DASTZR010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567184"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VFV20_09330"
     CDS             complement(<1..444)
                     /locus_tag="VFV20_09330"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEU5288593.1"
                     /translation="MVETLFIELVRSFVYDRFALDPVSATVAGVHTHDHELGDLSEQG
                     FGERRAFAEEWLARFLDTTEGLTPAQEIDRDLIVADLRGEVALHDFERWRRDPGVYSG
                     VVTRGAYYGILREYSQMEERLALLAERLQQAPAVLEAARANLDPAR"
     gene            543..854
                     /locus_tag="VFV20_09335"
     CDS             543..854
                     /locus_tag="VFV20_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288594.1"
                     /translation="MSDDDGWYRYADARPIYLEAGADVRIVGWAKQPDGRQPMLECTA
                     FDTKHLQCGVYGTRPEHCRLYDCRGEDEYAEEWQARPHCDLERHRRMEAMRARPARKS
                     A"
     gene            complement(851..1045)
                     /locus_tag="VFV20_09340"
     CDS             complement(851..1045)
                     /locus_tag="VFV20_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018001928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEU5288595.1"
                     /translation="MPFNLGGPELIFLLVIVLIVFGAGKLPDVFGQLGKGVRTFREEA
                     TNADKPAATSTATEEKKPTS"
     gene            complement(1099..1863)
                     /locus_tag="VFV20_09345"
     CDS             complement(1099..1863)
                     /locus_tag="VFV20_09345"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU5288596.1"
                     /translation="MTVVGAIALGVSVAAGVLALRADSRVARWVRVLSGTGARVRGAR
                     RGGEDLLVVRVAAACAGALIGSAIGAVVSVGALAVAALAYAGWVGPSVVAERRAADRR
                     RAAERGLVTLVEWTHALVSSGRPPEAAIADVTASGRVDAALRLSLDRVRREYVLGVPM
                     HTALAREATASGVRGLAELAGRLEQARDLGRAGLPLLADLRDDLRAQERARALAAAGA
                     VEGKLTAVMTLCYLPALALLVIVPLFVTLLAGLFGP"
     gene            complement(1860..>2326)
                     /locus_tag="VFV20_09350"
     CDS             complement(1860..>2326)
                     /locus_tag="VFV20_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288597.1"
                     /translation="RVALERVDAADRAPFERAMRAFELNEGLEDALAAARATAHDRRV
                     TLALDALALVASEQVPASRAAALVQSVADRLSFEERLLAEVRARTGGVRAQIVVLALL
                     VPALAAYLSLTMPGLATTLATPLGRLVLIPGAVVLEITGVLASRAIVRDLSR"
BASE COUNT          377 a          825 c          844 g          280 t
ORIGIN      
        1 gcgcgcggga tcgaggttcg cgcgggccgc ttcgagcacg gcgggcgcct gctgcagccg
       61 ctcggcgagc agcgcgagcc gctcctccat ctgcgagtac tcgcgcagga tgccgtaata
      121 cgcgccacgc gtgacgacgc cggagtacac gcccgggtcg cggcgccagc gctcgaagtc
      181 gtgcagcgcg acctcgccgc ggagatcggc gacgatgaga tccctgtcga tctcctgggc
      241 gggcgtgaga ccttcggtcg tgtcgaggaa gcgcgcgagc cactcctcgg cgaacgcgcg
      301 ccgctcgccg aagccctgct cgctcagatc gccgagctcg tgatcgtgcg tgtggacgcc
      361 ggcgacggtc gccgagacgg ggtcgagagc gaagcggtcg tagacgaacg agcgcaccag
      421 ctcgatgaag agggtctcta ccatgggatc gaggcgagtc tgtcacaggg gagcgggacg
      481 tggcgcgagc accgtatccg gactgcacca gctgcgaggc accatgctgc cgccgtcagt
      541 tcatgagcga cgacgacggg tggtaccgct acgccgacgc gcgcccgatc tacctcgagg
      601 ccggcgcgga cgtgcgcatc gtcggatggg cgaagcagcc cgatgggcgg cagccgatgc
      661 tcgagtgcac ggccttcgat acgaaacatc tgcagtgcgg cgtgtacggg acgcggccgg
      721 agcactgccg gctgtacgac tgcaggggcg aggatgaata cgccgaggag tggcaggcgc
      781 ggccgcactg cgatctcgag cggcatcgcc gtatggaggc gatgcgcgct cgacccgcgc
      841 ggaagagcgc ctagctcgtc ggcttcttct cttcggtcgc cgtgctcgtc gcggccggct
      901 tgtcggcgtt cgtcgcttcc tcacggaacg tgcgcacgcc cttgccgagc tgtccgaata
      961 catccgggag cttccccgct ccgaagacga tcagcacgat caccaggagg aagatcagct
     1021 caggtccgcc aaggttgaac ggcattcgtc acttcccttt cgggcctctg cccggtgttg
     1081 ccaacagtgt acgtccgcct agggcccaaa aaggcccgcg agcaacgtaa cgaagagcgg
     1141 cacgatgacg aggagggcga gcgccgggag gtaacaaagc gtcatcaccg cggtcagctt
     1201 tccctcgacg gcaccagcgg cggcgagggc gcgtgcgcgc tcctgcgcgc gcaggtcgtc
     1261 gcgaaggtcc gcgaggagcg gcaggcccgc ccgcccgaga tcgcgggctt gctcgagacg
     1321 ccccgcgagc tcggcgaggc cgcggacgcc ggacgcggtc gcctcacgag cgagcgcggt
     1381 gtgcatcggc acgccgagaa cgtactcgcg ccgcacacga tccagcgaaa ggcgtagcgc
     1441 ggcgtccacg cgaccgctcg ccgtgacatc ggcgatcgcg gcttccggcg gccttcccga
     1501 cgacacgagc gcgtgcgtcc attcgacgag cgtgacgagc ccgcgctcgg cggcccgccg
     1561 ccggtctgcc gcgcgccgct ccgcgaccac cgaaggcccg acccaccccg cgtaggcgag
     1621 cgccgcgacc gcgagcgcgc cgaccgacac gacggcgccg atcgcggagc caatgagcgc
     1681 gccggcgcac gcggccgcca cgcggaccac gagcaggtcc tctccgccgc gacgcgctcc
     1741 tcggacgcgc gcgccggtgc cgctcagcac gcgtacccat cgtgcgacgc gcgaatccgc
     1801 gcgcagggcg agcacgccgg ccgcgacgga cacgccgaga gcgatcgcgc cgacgacggt
     1861 catcgcgaca gatcgcgcac gatcgcgcgg ctcgcgagga cgcctgtgat ctcgagcacg
     1921 accgcgcccg ggatgagaac gagccggccg agcggtgtgg caagcgtcgt cgcgagcccc
     1981 ggcatggtga gtgagagata cgccgcgagg gcgggaacga gcagcgcgag gacgacgatc
     2041 tgcgcgcgca cgccgcccgt ccgcgcccgc acctcggcga gcagccgttc ctcgaacgac
     2101 agccgatcgg cgacgctctg gacgagcgcg gcggcgcgcg acgcgggcac ctgctcggat
     2161 gccacgagcg cgagcgcgtc gagcgcgagc gtgacacgcc ggtcgtgcgc ggtcgcgcgc
     2221 gccgcggcga gcgcatcctc gagaccctcg ttgagctcga acgcccgcat cgcgcgctcg
     2281 aagggggcgc gatcagcggc gtcgacacgc tcgagcgcga cgcgaa
//
LOCUS       DASTZR010000421         3089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_963383, whole genome shotgun sequence.
ACCESSION   DASTZR010000421 DASTZR010000000
VERSION     DASTZR010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963383"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1240)
                     /locus_tag="VFV20_09355"
     CDS             complement(<1..1240)
                     /locus_tag="VFV20_09355"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEU5288598.1"
                     /translation="MSADASWFERQFKVRAAGSTIGTEIRGGLTNYLVMSYILVVNAV
                     VLGNAGIPFPAVVAATAIMAAIFTGGMALWANYPYAGAAGLGINAVVAFGLVKGAGLS
                     WQGAMGVVALEGLAICVAMALGVRRALLDAVPVPIKLGIGAGIGAFIFTIGAFNGGLA
                     VGTGNPADPVALGNLNSATAIVTLGGLLLTAFLYARNVKGALILGIIGATIIGIAVNA
                     ATGGAAYKDLPGVAVMPKSFFDTPDFSTIGAIFTTVGGNIALLEVFRLGFFVAILTIF
                     SLMLSDFFDTAGTVVSVASEGGWLKPNGTIERADRVFWVDSLGAMFGGIFGTSSNTTY
                     IESGAGVAEGAKTGLASLVTAILFLLSVLLAPIAGIVPPQATAPALMLVGWFMFRPIL
                     DLNKIGNIDFATGFAVLLTIF"
     gene            complement(1405..1839)
                     /locus_tag="VFV20_09360"
     CDS             complement(1405..1839)
                     /locus_tag="VFV20_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HEU5288599.1"
                     /translation="MPSLYDRLGGVFAIAAVVDHFSDNIVENPIVGKTSKNPALREWH
                     THSLDRLPGLKFMRTLWVCNVAGGPFQYTATRPGDTTLGLEEAHRPLEISPAEFDEVA
                     AELGRTLDHFKVPKAEKEEVLAAFAAHKGEVTEGYAEHAGRR"
     gene            1964..2602
                     /locus_tag="VFV20_09365"
     CDS             1964..2602
                     /locus_tag="VFV20_09365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5288600.1"
                     /translation="MATARRNGTLDIRPLSPSRVADVRTVLASTFGSQCWDLFFRFTD
                     EQKRAAGIAGKLTPKNEARRKEVLAKLARRKRGSAGLVAYADGEPVGFISLGPRSDYP
                     RVERSQAMSAVDDVPVWVIPCITVRKGYRGKGVAIAMLRATVAYAAKKGAPAVEGYAR
                     PDGERVHDGSAHMGTVSMFRRAGFKRIRGVVPGLPRYYTPRVTMRATSRRSG"
     gene            2746..>3089
                     /locus_tag="VFV20_09370"
     CDS             2746..>3089
                     /locus_tag="VFV20_09370"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5288601.1"
                     /translation="MNLNSVLIGSEDPKRLTDYYTKVFGKPSMEDGGFAGWKIGDGWI
                     TVGPHSEIKGKNPQPGRILLNIESSDVKGDFAKLKTAGAKVVREPYRMEGWPENTWVA
                     TLADPDDNYFQLV"
BASE COUNT          525 a         1020 c         1097 g          447 t
ORIGIN      
        1 cgaagatggt cagcagcacc gcgaagccgg tcgcgaagtc gatgttgccg atcttgttca
       61 ggtccaagat cggccggaac atgaaccacc cgaccagcat gagggcgggc gcggtcgcct
      121 gcggcggcac gatgcccgcg atcggcgcga ggaggacgga gagcaggaac aggatcgcgg
      181 tgacgaggct cgcgagcccc gtcttggcgc cctccgcgac gccggcgccg gactcgatgt
      241 aggtcgtgtt gctcgacgtg ccgaagatgc cgccgaacat ggcgccgagc gagtcgaccc
      301 agaagacgcg gtcggcacgt tcgatcgtgc cgttcggctt cagccaaccg ccctcggacg
      361 cgaccgacac gaccgtgccg gcggtgtcga agaagtcgga gagcatgagc gagaagatcg
      421 tgaggatcgc gacgaagaat ccgagccgga agacctcgag gagcgcgatg ttgccaccga
      481 cggtggtgaa gatcgcgccg atggtcgaga agtcgggcgt gtcgaagaac gacttcggca
      541 tcaccgcgac gccggggaga tctttgtagg ccgcgccacc ggtcgccgca ttcacggcga
      601 tgccgatgat cgtcgcgccg atgatgccga ggatgagcgc gcccttcacg ttgcgggcgt
      661 acaggaacgc ggtgagcaga agaccgccga gggtgacgat cgcggtcgcg ctgttcaggt
      721 tgccgagcgc caccggatcg gccgggttgc ccgtgccgac ggcgagcccg ccgttgaagg
      781 caccgatggt gaagatgaag gcgccgatgc cggcgccgat gccgagcttg atcgggacgg
      841 gcaccgcgtc caggagcgcg cggcgtacgc cgagcgccat cgcgacgcag atcgcgagac
      901 cttcgagggc cacgacgccc atggcgccct gccacgacag gccggcgccc ttgaccaggc
      961 cgaacgcgac caccgcgttg atgccgaggc cggcggcgcc cgcgtacggg tagttggccc
     1021 agagcgccat gccgccggtg aagatcgccg ccatgatcgc ggtggccgcg accaccgccg
     1081 ggaacgggat gcccgcgttg ccgagcacga ccgcgttcac cacgaggatg taggacatga
     1141 cgaggtagtt ggtgagtccg ccgcggatct ccgtgccgat ggtcgaaccc gcggcgcgga
     1201 ccttgaactg gcgctcgaac cagctcgcgt cagcgctcac tgcgctgctc cttgctgcca
     1261 tctttccctc ccttgcgcgt cggaccgctg caagccgtgc cggccatgac acctccgcga
     1321 cgaggctcac accggcacgc gggaaagcac gacaggaagc gctgcggatt ctacaggccg
     1381 gtcggtcgta cgcccggtcg accgtcagcg gcggccggcg tgctccgcgt agccctcggt
     1441 cacctcgccc ttgtgcgcgg cgaacgcggc gaggacctct tccttctcgg ccttcggcac
     1501 cttgaagtga tcgagcgtgc ggcccagctc agcagcgacc tcgtcgaact ccgcgggcga
     1561 gatctcgagc gggcgatgcg cctcctcgag gccgagcgtc gtgtcgccgg gccgcgtcgc
     1621 ggtgtactgg aacgggccac cggccacgtt gcagacccac agcgtgcgca tgaacttgag
     1681 gcccggcagc cggtcgaggc tgtgcgtgtg ccactcgcgc agcgccgggt tcttcgaggt
     1741 cttgcccacg atggggttct cgacgatgtt gtcgctgaag tggtctacca ccgccgcgat
     1801 cgcgaagacg ccaccgaggc ggtcgtacag gctgggcatg gctgtccctc cgagtgcggt
     1861 ttctcgcgtt ggggatacga agcacggccg cattccggag gcgagggacg cgtagacctg
     1921 tgcaggcagg ggtgattggt tgcctgtgag cgatactgcc gcgatggcga cggcgcgccg
     1981 caacggtacc ctcgatatcc gtccgctgtc cccgtcacgg gtcgcggacg tccgcaccgt
     2041 gctggcgagc acgttcggct cgcagtgctg ggacctgttc ttccgcttca cggacgagca
     2101 gaagcgagca gccgggatcg ccgggaagct gacaccgaag aacgaggcgc gccgcaagga
     2161 ggtcctggcg aagctggcgc ggcggaagcg gggcagcgcg ggcctcgtcg catacgcgga
     2221 cggcgagccc gtgggattca tctcgctcgg accgcgctcc gactatcccc gcgtcgagcg
     2281 atcgcaggcg atgtccgccg tcgacgacgt cccggtatgg gtcatcccct gcatcaccgt
     2341 ccggaaggga taccgcggca aaggcgtggc gatcgcgatg ctccgggcga ccgtcgcgta
     2401 tgccgcgaag aagggcgccc ccgcggtcga gggctatgcg cgtcccgacg gcgagcgcgt
     2461 ccacgacggc tccgcgcaca tgggcaccgt gtcgatgttc cggcgggccg gattcaagcg
     2521 catccgcggc gtcgtgccgg gcctgccccg ctactacacg ccgcgcgtga cgatgcgcgc
     2581 gacctcgcgc cgcagcggct gacgcgcacg cacccgtccc tcaacggcag gctgcagggc
     2641 gagtgccgtc ttggccgtcg cgcccgatgg gcacccgtac actcggcggc gcgccggcga
     2701 tgagttccga ccactctcac cgtcgaacca acaggaggtg gcgatatgaa tctgaacagc
     2761 gttctcatcg gctccgagga cccgaagcgc ctgaccgact actacacgaa ggtattcggt
     2821 aagccgtcga tggaggacgg tggcttcgct ggctggaaga tcggcgacgg ctggatcacg
     2881 gtcgggccgc acagcgagat caaggggaag aacccacagc cggggcggat cctcctcaac
     2941 atcgagagct cggacgtgaa gggcgacttt gccaagctga agacggccgg ggcgaaggtc
     3001 gtgcgcgagc cctaccggat ggaagggtgg ccggagaaca cctgggtcgc gacgctggcc
     3061 gacccagacg acaactactt ccagctcgt
//
LOCUS       DASTZR010000422         2713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_885035, whole genome shotgun sequence.
ACCESSION   DASTZR010000422 DASTZR010000000
VERSION     DASTZR010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885035"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2713)
                     /locus_tag="VFV20_09375"
     CDS             complement(<1..>2713)
                     /locus_tag="VFV20_09375"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEU5288602.1"
                     /translation="TGADGTATVTYKPTKAGTLRLVAIATDRTGRTSRSATYLWVSGA
                     GFALWQVTNDDTIKLVADKERYEVGDTAEILVPAPFAGATALVTVERGKIITREVRTL
                     PTNSERLRIPIVDHSVPDVFVSVVLYRGPTAEDPLPRYKVGYTQLPVDTSIRKLNVKI
                     TPDRAQAQPGETVRYAIAVTDRNGRGVRSQLSVAVVDKAVLSLMEERGPDGMRAFWFE
                     RGLAVNTNSSMGVSVDRWNDVIAELPRVGKGGSGSGLTGDRERQDYRNTAYWESQLVT
                     KDDGTATVDVVMPDDLTTWRLQARAISGDTMVGEGTNELVSTKPLLIRSALPRFLRTG
                     DEVDLRVLVRNGTGAARRVPVTLKAEGPIAVSGALTQTQDLSSDGRSFAYVWRAKVQG
                     EGTVTVSVSGAAGKDSDAMKVSLPAYIDLTPETMSTGGVVTKEAGLEAIYLPKFADTA
                     HGTLGVGVRSSLVGSLASELPSFDRRPPFSYPEDTMSVASRVIATMAVARADAAAGTG
                     KSYDSRITTDLAELIGRQRPDGGWPWCIAPECGTDPNVTGWVLLALGEAHREQRSVDP
                     AVVGRASSYVYAWLNRPNSTVNPTTSDQDQRAYMLAALAAAGSTSAWNNANALFEQYR
                     TQLANWGRAYLIGALVDSGGKTEDTQPRALLNDLAASTIPSANGNHWEDPATAAKSSF
                     MTSTATTALVALAIARVQPEHQLLAQTVRWLTVARGGQGWQTSVERAMSILALATYVQ
                     LTGEQGTEFGYRVALDEKMVLTGTVRKSTTPTEDSTRIPLTRVTPGKASVISVEREFA
                     KTGRLYYTLDLRYMTPAKDIEAVNRGFGLSHTYTLLDDPSRPVTSAKLGEVVRVTLTV
                     LAPSDRSYVTIEDLLPAGLEAIDARLRTTDPSLKKKLEDERVAAFQRG"
BASE COUNT          348 a          955 c          971 g          439 t
ORIGIN      
        1 gccgcgctgg aacgccgcga cgcgctcgtc ctcgagcttc ttcttcagtg acggatcggt
       61 ggtccggagg cgcgcgtcga tcgcctcgag tcccgcaggg agtaagtcct cgatcgtgac
      121 gtagctccgg tcgctcggcg cgagcaccgt gagcgtcacg cgcacgacct cgccgagctt
      181 cgcgctcgtc accggacgcg acgggtcgtc gagcagcgtg taggtgtgcg acaggccgaa
      241 cccgcggttc accgcctcga tgtccttggc cggcgtcatg tagcgcaggt cgagcgtgta
      301 gtagaggcgc ccggtcttcg cgaactcgcg ctccaccgag atgacgctcg ccttgcccgg
      361 cgtgaccctg gtcaatggga tgcgcgtgct gtcctcggtg ggcgtcgtgc tcttccggac
      421 cgtgcccgtc agcaccatct tctcgtcgag cgcgacgcgg tagccgaact cggtgccctg
      481 ctcgccggtg agctgcacgt aggtcgcgag cgcgaggatg ctcatcgccc gctcgacgct
      541 ggtctgccag ccctgcccgc cgcgcgcgac ggtgagccag cgcaccgtct gcgcgagcag
      601 ctgatgctcg ggctgcaccc gcgcgatcgc gagcgcgacg agcgcggtcg tggccgtgct
      661 cgtcatgaac gagctcttcg cggcggtcgc cgggtcctcc cagtgattgc cgttggcgct
      721 cgggatggtc gaagcggcga ggtcgttgag gagggcccgc ggctgcgtgt cctcggtctt
      781 gccgccgctg tccacgagcg cgccgatgag gtacgcgcgg ccccagttcg cgagctgcgt
      841 acggtactgc tcgaagagcg cgttcgcgtt gttccacgcg ctcgtgctgc ccgccgcggc
      901 gagcgccgcg agcatgtacg cccgctggtc ctggtcactc gtcgtcgggt tcaccgtcga
      961 gttcgggcga ttcagccacg cgtacacgta ggagctcgcg cggcccacga cggccggatc
     1021 gaccgagcgc tgctcgcgat gcgcctcgcc gagcgcgagg agcacccagc cggtgacgtt
     1081 cggatcggtc ccgcactcgg gcgcgatgca ccacggccaa ccgccgtcgg gccgctgccg
     1141 gccgatgagc tcggccaggt ccgtggtgat gcgcgagtcg tagctcttgc cggtgcccgc
     1201 cgcggcgtcg gcgcgcgcga cggccatcgt cgcgatgacc ctgctcgcca cggacatggt
     1261 gtcctcggga tacgagaacg gcggccgacg gtcgaacgac ggcagctcgc tcgcgagcga
     1321 gcccacgagc gacgagcgca cgcccacgcc gagcgtgccg tgcgcggtgt ccgcgaactt
     1381 cggcaggtag atcgcctcga gaccggcctc cttcgtcacg acgccgccgg tcgacatcgt
     1441 ctccggcgtg agatcgatgt acgcgggcag gctcaccttc atcgcgtcac tgtctttccc
     1501 ggcggccccg ctgacgctga ccgtcacggt gccctcgccc tgcaccttcg cgcgccagac
     1561 gtacgcgaac gagcgtccgt ccgacgagag gtcctgcgtc tgggtgagcg cgccggagac
     1621 cgcgatcgga ccctcggcct tcagcgtgac cggcacgcgc cgcgcggcgc cggtgccgtt
     1681 gcgcaccagg acgcggagat ccacctcgtc gccggtgcgg aggaagcgcg gcagcgcgga
     1741 gcggatgagg agcggcttcg tcgacacgag ctcgttcgtg ccctcgccga ccatcgtgtc
     1801 gccgctgatg gcgcgcgcct gcaggcgcca ggtggtgaga tcgtcgggca tgacgacgtc
     1861 cacggtggcc gtgccgtcgt ccttcgtcac gagctgcgac tcccagtacg cggtgttgcg
     1921 gtagtcctgg cgttcgcggt cgccggtgag accgctgccg ctgccgccct tgcccacgcg
     1981 cggcagctcg gcgatgacgt cgttccagcg atcgacggac acgcccatcg acgagttcgt
     2041 gttcaccgcg aggccgcgct cgaaccagaa cgcgcgcatg ccgtcgggac cgcgctcctc
     2101 catgaggctg agcacggcct tgtcgacgac cgcgaccgag agctgtgagc gcacaccccg
     2161 gccgttccgg tcggtcaccg cgatggcgta gcggacggtc tcgcccggct gcgcctgcgc
     2221 gcggtccggc gtgatcttca cgttcagctt gcggatcgag gtgtcgaccg gcagctgcgt
     2281 gtagccgacc ttgtagcggg ggagcggatc ctccgccgtc gggccgcggt agagcacgac
     2341 cgacacgaac acgtcgggca cggagtggtc gacgatcggg atgcgcaggc gctcgctgtt
     2401 ggtcgggagc gtgcgcacct cgcgcgtgat gatcttgccg cgctcgaccg tgacgagcgc
     2461 ggtcgcgccg gcgaacggag cgggcacgag gatctcggcg gtgtcgccca cctcgtagcg
     2521 ctccttgtcg gcgacgagct tgatggtgtc gtcgttggtg acctgccaca gcgcgaagcc
     2581 cgcgccgctg acccagaggt acgtcgccga gcgcgaggtg cggcccgtcc ggtccgtcgc
     2641 gatggcgacc aggcgcagcg tcccggcctt cgtcggcttg taggtcacgg tcgccgtccc
     2701 gtcggcgccg gtg
//
LOCUS       DASTZR010000423         3842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1198378, whole genome shotgun sequence.
ACCESSION   DASTZR010000423 DASTZR010000000
VERSION     DASTZR010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198378"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..924)
                     /locus_tag="VFV20_09380"
     CDS             complement(55..924)
                     /locus_tag="VFV20_09380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEU5288603.1"
                     /translation="MPRSIWRGVLSFGMVAIPVRLYLATESSSKVSFHLLCPEHKSRI
                     KNKRWCQDGDHEVAWNDVVRAYEYEKGSYVELDDEDLEKLPLRSSKAIDISGFIERSD
                     LAEELYIQSAYYLEPEKAAAKPYALLKKTLDKTGRLAIAKFALRDRERLVSIGAHDGA
                     LVLNTLHWPDEIRSTEELDIDTPSVSPAELKMAENLVNMMATKFEPAEYKDEYKQALQ
                     EVVEAKVEKREVIEAPEPEAETTVVDLMAALKASVEKAKKAEQAGGRTKRGGHESLVH
                     RKPARTEKRRKAS"
     gene            complement(1027..1581)
                     /locus_tag="VFV20_09385"
     CDS             complement(1027..1581)
                     /locus_tag="VFV20_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288604.1"
                     /translation="MSPTYLALLALGIALVAVARWAGRRRGSRWDSLVALRVGDILLT
                     EVAGAFLIGFSLGNLIAPPGPQIGRPGPPRGVRFGPGGGITLPIGLGLLGAAIAVLVH
                     VDLRDIFLAGRAARNPLVTYIGWDARVIAPIPAGGFGEIAMRDGAGNVMSVVATADVD
                     IAEGTTVRVTAVRHLNLVVSPLTG"
     gene            complement(1587..3212)
                     /locus_tag="VFV20_09390"
     CDS             complement(1587..3212)
                     /locus_tag="VFV20_09390"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEU5288605.1"
                     /translation="MASSFAGLVETFLKEEYEDSPTLASSLGLTEYDEKLDITTADRF
                     RWRVERDESWLARFRALPDADLGPEERIDRDLLVSVLRGRQLAVPLESWRRQPATYLN
                     PGLQGVFSLFLHRLRPEKELADAAASRLWQVPRMIADGVANLDLPRTPRVYLERAIGQ
                     AKAAAKYARELVPAEVKDTAAKTKLAEAGAAAGKAFDEFAAFLETHRGDAGGDYAIGE
                     ELYSALLREKELLPFGTRELRERGREQWDLLSTEADRIAHEIDGGTWRETCERLNKIH
                     AATPEGMRAEYAEWTERARSFLRDTGLVTLPSGEECSVEPSPPFQRPILAVASYNRPP
                     AFKDTLHGHFFVPYPPDGTSPEEVQKRLEGNCSAGIPTTAVHEAYPGHHWHLVMAKAN
                     PSHVRRIFGTPYFSEGWALYAERVMREQGFFSDPKHLLYQYEATIFRAARIVVDTSLH
                     MGEMSFDEAVRFMVEKGNLTEPNAKAEVGRYCSWPTQASSYLTGMLEIVDIRTRWLAR
                     RGSSDRAALRAFHDAITSAGMLPTTLAERAISA"
     gene            3286..>3842
                     /locus_tag="VFV20_09395"
     CDS             3286..>3842
                     /locus_tag="VFV20_09395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEU5288606.1"
                     /translation="MSLTEYQRKRDFRCTPEPKGREPEATPSRRYVVHRHHATRLHWD
                     VRLEMHGVLASWAVPQGPPLEAGKRRLAVHTEDHPLEYLTFHGVIPDGYGAGTMTIWD
                     EGTYDLLKDSPAEYKVHLHGKRLEGEWVIVRTKQNEGRDWLMIKHGVAPKDHPLDAKI
                     EPMLAISADAPFDSAEFAFEAKWDGV"
BASE COUNT          580 a         1326 c         1327 g          609 t
ORIGIN      
        1 cacgcgccga gcataggagg tgacacgccg gcctctgacc cgcgctcggt agcgctatga
       61 ggccttgcgg cgcttctccg ttcgggccgg cttgcgatgc acgagcgact cgtggccgcc
      121 gcgcttcgtg cgtccgccag cctgctcggc cttcttcgcc ttctcgacgc tcgccttgag
      181 cgcggccatg aggtcgacga ccgtggtctc ggcctcgggc tcgggggcct cgatgacctc
      241 gcgcttttcg accttggcct cgacgacctc ctgcaacgcc tgcttgtact cgtccttgta
      301 ctccgcgggc tcgaacttcg tggccatcat gttcacgagg ttctccgcca tcttcagctc
      361 cgcgggcgag acgctgggcg tgtcgatgtc gagctcttcg gtcgaacgga tctcgtcggg
      421 ccagtgcagc gtgttcagca cgagggcgcc gtcatgagcg ccgatcgaca cgaggcgctc
      481 ccggtcacgc agcgcgaact tcgcgatcgc gaggcggccg gtcttgtcga gggtcttctt
      541 cagcagcgcg tacggcttgg cggcagcctt ctccggctcg aggtagtacg cggactggat
      601 gtacagctcc tcggcgaggt cggatcgctc gatgaagcca ctgatgtcga tggccttgct
      661 cgagcggagc gggagcttct cgaggtcctc gtcgtcgagc tcgacgtagc tgcccttctc
      721 gtattcatac gcgcgcacca cgtcgttcca cgcgacctcg tggtcgccgt cctgacacca
      781 gcgcttgttc ttgatgcgcg acttgtgctc ggggcacagc agatggaacg agaccttgct
      841 cgagctctcc gtggcgagat acagacgtac ggggatcgcc accatcccga acgacagcac
      901 gccgcgccag atggaacggg gcatcggtca cctccagccg cttcgccctc tgttgcgcgc
      961 ggcctgccgc gcgaatgtaa cggccgtgtc aaggtagagc agccggcgtg ccgccggaag
     1021 tcgcgctcag cccgtcagcg gcgacacgac gaggttgagg tggcggaccg ccgtgacgcg
     1081 gacggtggtg ccctccgcga tgtcgacgtc ggcggtcgcg acgacggaca tcacgttccc
     1141 cgcgccgtcg cgcatcgcga tctcgccgaa gcctcccgcg gggatgggtg cgatgacgcg
     1201 cgcgtcccag ccgatgtacg tgacgagcgg attgcgagcg gcacggccgg cgagaaagat
     1261 gtcgcgaaga tcgacgtgca cgagcacggc gatcgccgcg ccgagcagac cgagaccgat
     1321 cggcagcgtg atgccgccgc ctggcccgaa gcggacaccg cgcggcggtc cggggcggcc
     1381 gatctgcggg cccgggggcg cgatcaggtt cccgagcgag aatccgatga ggaacgcacc
     1441 ggccacctcc gtcagaagga tgtcgccgac gcgcagtgcg accagtgagt cccacctgct
     1501 cccgcgccgc cggcccgccc accgcgcgac cgcgacgagc gcgatcccaa gggcgagcag
     1561 ggcgagatag gtcggcgaca tcggcgctag gccgagatgg cgcgctccgc gagcgtcgtc
     1621 ggcagcatgc cggcgctcgt gatcgcgtcg tggaatgcgc gcagggccgc gcgatcggac
     1681 gagccgcgcc gggcgagcca gcgcgtgcgg atgtccacga tctcgagcat gccggtgaga
     1741 tagctcgacg cctgcgtcgg ccacgagcag tagcggccga cctcggcttt cgcgttcggc
     1801 tcggtcaggt tgcccttctc gaccatgaat cgcaccgcct cgtcgaagct catctcgccc
     1861 atgtgcaggc tcgtgtcgac cacgatgcgc gcggcgcgga agatcgtggc ctcgtactga
     1921 tagagcaggt gcttcggatc gctgaagaag ccctgctcgc gcatcacgcg ctcggcgtac
     1981 agcgcccacc cctcgctgaa gtacggggtg ccgaagatgc gccgcacatg tgacggattc
     2041 gccttcgcca tcaccaggtg ccagtggtgc cccggatagg cctcgtggac cgcggtcgtc
     2101 gggatgcccg cgctgcagtt gccctcgagg cgcttctgca cttcctccgg tgacgttcca
     2161 tccggcggat acgggacgaa gaagtgcccg tgcagcgtgt ccttgaacgc cggcgggcgg
     2221 ttgtacgaag cgacggccag gatggggcgc tggaacggcg gcgacggctc gaccgagcat
     2281 tcctcgccgc tcggcagcgt gacgagtccg gtgtcgcgca ggaacgagcg cgcgcgctcc
     2341 gtccactccg cgtactccgc gcgcatccct tcaggtgttg cggcgtggat cttgttcagg
     2401 cgttcgcagg tctcgcgcca cgtgccgccg tcgatctcgt gcgcgatccg gtccgcctcg
     2461 gtggacagga gatcccactg ctcccgtccg cgctcgcgca gctcgcgcgt gccgaagggg
     2521 agcagctcct tctcacggag caacgcgctg tacagctcct cgccgatggc gtagtcgcca
     2581 cccgcgtcgc cgcggtgtgt ctcgaggaac gccgcgaact cgtcgaaggc cttgcccgcc
     2641 gccgctcccg cctccgcgag cttcgtcttg gcggccgtgt ccttgacctc ggcggggacg
     2701 agctcacgcg cgtacttcgc cgcggccttc gcctggccga tcgcgcgctc caggtacacc
     2761 cgcggcgtcc gcgggaggtc gaggttcgcg acgccgtcgg cgatcatgcg cggcacctgc
     2821 cagaggcgcg aggcggccgc gtcggcgagc tccttctccg gacggagccg atgcaggaac
     2881 agcgagaaca cgccctgcag tcccggattg agatacgtcg ccggctgccg ccgccaggac
     2941 tcgagcggca ccgcgagctg ccggccgcgg agcacggaga ccagcaggtc ccgatcgatc
     3001 cgctcctcgg ggccgaggtc cgcgtcgggg agggcgcgga accgtgcgag ccaggattcg
     3061 tcgcgctcga cccgccagcg gaagcggtcg gcggtcgtga tgtcgagctt ctcgtcgtac
     3121 tcggtcagac cgaggctcga cgcgagcgtc ggcgaatcct cgtattcctc cttgaggaac
     3181 gtctccacga gtccagcgaa cgacgacgcc acctgcgtcc tcctgcggtc gagtagacgg
     3241 ggcccctgct ccccgcctcc gggacaatgg ctgcgggagt agagcgtgag cctcaccgag
     3301 taccagcgca agcgagactt ccgatgcacg ccggagccga aggggcgcga gcccgaggcg
     3361 acgccgtcgc ggcggtatgt cgtgcaccgc caccacgcca cgcgcctgca ttgggacgta
     3421 cgcctcgaga tgcacggggt cctcgcctcg tgggcggtcc cgcagggccc gccgctcgag
     3481 gcggggaagc gacgactcgc cgtccacacc gaggatcacc cgctcgagta cctcacgttc
     3541 cacggcgtca ttcccgacgg ctacggcgcg ggcacgatga ccatctggga cgagggcacg
     3601 tacgacctgc tcaaggacag ccccgccgaa tacaaggtgc acttgcacgg gaagcggctc
     3661 gagggcgagt gggtcatcgt gcggacgaag cagaacgagg gccgcgactg gctgatgatc
     3721 aagcacggcg tcgcgccgaa ggatcatccg ctcgacgcga agatcgagcc gatgctcgcg
     3781 atcagcgcgg acgcgccgtt cgattccgcg gagttcgcgt tcgaggcgaa gtgggacggc
     3841 gt
//
LOCUS       DASTZR010000424         2504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1093745, whole genome shotgun sequence.
ACCESSION   DASTZR010000424 DASTZR010000000
VERSION     DASTZR010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093745"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1739
                     /gene="ftsH"
                     /locus_tag="VFV20_09400"
     CDS             <1..1739
                     /gene="ftsH"
                     /locus_tag="VFV20_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU5288607.1"
                     /translation="VPAGTTGKFSTLAPPQYERLANLLDEKGVIITAKKEATSPWASL
                     LYAWAPILLMIGFWIFIMRQMQSGGNKALSFGKSRAKLSSSSQKKVTFKDVSGVDEAK
                     EELQEIIEFLKEPQKFQKLGGRIPKGVLLMGPPGTGKTLLARAVAGEANVPFFSISGS
                     DFVEMFVGVGASRVRDLFEQGKKNAPCIVFIDEIDAVGRHRGAGLGGGHDEREQTLNQ
                     LLVEMDGFESNEGVILVAATNRPDVLDPALLRPGRFDRRIVVNRPDVKGREGILAVHT
                     KKIPLADDVNIHILARGTSGFSGADIANLVNEAALNAARYNQKVVRMLDFEFAKDKVL
                     MGSERRSMIINDDEKKVTAVHEAGHALLAVLLPHADPVHKVTIIPRGMALGVTMQLPV
                     DDKHNYTRDYLNDQIAILLGGRIAEEITMSSMTTGAGNDLERSTELARKMVCEWGMSD
                     AMGPLTFGKKEEQIFLGREIAQHQDYSEDTAIHIDQEIKRVVITNYDRARLLLEQHRP
                     ALGRIAEELLTREVLDADQVRRIVAGDALEEHKPAAAAAAPLPDDGRRQAKERPSIVP
                     PIPPLNKPVTQE"
     gene            1822..>2504
                     /gene="folP"
                     /locus_tag="VFV20_09405"
     CDS             1822..>2504
                     /gene="folP"
                     /locus_tag="VFV20_09405"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU5288608.1"
                     /translation="MPLPGGRSMELGARTLVMGILNITPDSFADGGMHFDHERAVASG
                     LQMVADGADILDIGGESTRPGADPVGAGEEMRRVLPVIERLAAQCPALISIDTYKGAV
                     AREAVARGASIINDISGLQYDPDLARAAAEAGAGLVLMHTRGRSRDMYEHAVYEDVVE
                     EVVRELDASIGRATAEGIRRDAIVVDPGFGFAKRAEHSYEALARLDRLRVLDRPVLSG
                     PSRKSFLKAA"
BASE COUNT          439 a          855 c          836 g          374 t
ORIGIN      
        1 cggtccccgc cggcaccacg ggcaagttca gtaccctcgc ccccccgcag tacgagcggc
       61 ttgcgaacct tctcgacgag aagggcgtca tcatcacggc gaaaaaggag gccaccagcc
      121 cgtgggcgtc gctgctctac gcctgggctc cgatcctgct gatgatcggg ttctggattt
      181 tcatcatgcg gcagatgcag agcggcggga acaaggccct gtcgttcggc aagagccgcg
      241 cgaagctctc ctccagctcg cagaagaagg tcacgttcaa ggacgtgtcc ggcgtggacg
      301 aagcgaagga agagctccag gaaatcatcg agttcctgaa ggaaccgcag aaattccaga
      361 agctcggcgg ccgcattccg aagggcgtcc tgctcatggg ccctcccgga accggcaaga
      421 cgctgctcgc gcgcgccgtc gccggtgaag cgaacgttcc gttcttctcg atcagcgggt
      481 cggacttcgt cgagatgttc gtcggcgtcg gcgcctcgcg cgtccgcgac ctcttcgagc
      541 aggggaagaa gaacgccccc tgcatcgtgt tcatcgacga gatcgacgcc gtcgggcggc
      601 accgcggcgc gggccttggc ggcggccacg acgagcgcga gcagacgctc aaccagctgc
      661 tcgtcgagat ggacggcttc gagtcgaacg agggcgtcat cctcgtggcg gccacgaacc
      721 gtcccgacgt tctcgatccg gcgctcctcc gtcctggccg attcgaccgc cgcatcgtcg
      781 tcaaccgtcc ggacgtgaaa gggcgcgagg ggatcctcgc cgtccacacc aagaagatcc
      841 cgctcgccga cgatgtgaac attcacatcc tcgcgcgcgg cacctccgga ttctccgggg
      901 ccgacatcgc caacctggtg aacgaagccg cgctgaacgc cgcgcgctac aaccagaaag
      961 tcgtccggat gctcgacttc gagttcgcga aggacaaggt cctcatgggc tcggagcggc
     1021 gctcgatgat catcaacgac gacgagaaga aggtgacggc ggtccacgag gcgggccacg
     1081 cgctgctcgc cgtgctgctg ccgcacgccg atcccgtcca caaggtgacg atcatcccgc
     1141 gcggcatggc gctcggcgtc accatgcagc tgccggtgga cgacaagcac aactacacgc
     1201 gcgactacct gaacgaccag atcgcgatcc tgctcggcgg ccgcatcgcc gaggagatca
     1261 cgatgtcgag catgacgacc ggcgccggca acgacctcga gcgctcgacg gagctggcgc
     1321 gcaagatggt ctgcgagtgg ggcatgagcg acgccatggg tccgctcacc ttcggcaaga
     1381 aggaagagca gatcttcctc ggccgcgaga tcgcgcagca ccaggactac agcgaagaca
     1441 cggcgattca catcgaccag gaaatcaagc gcgtcgtgat caccaattac gaccgcgcgc
     1501 ggctgctgct cgagcagcac cgtccggcgc tcggccggat tgccgaggag ctgctcacgc
     1561 gcgaggtgct cgacgccgac caggtccgcc gcatcgtcgc gggcgacgcg ctcgaagagc
     1621 acaagccggc cgccgcggcc gcggctccgc tgccggatga cggccgccgc caggcgaagg
     1681 agcggccgtc gatcgtgccg ccgattccgc cgctgaacaa gcccgtcacg caggagtagg
     1741 gcgcctgtag gccggggctc tctggtgtag gccggggctc ttagccccgg catcctcatg
     1801 cctcctcgaa aactttacgt cgtgcccctc cccggcggcc gctccatgga gctcggagcg
     1861 cgcacgctcg tcatgggcat cctcaacatc acgccggact cgttcgcgga cggcggcatg
     1921 cacttcgacc acgaacgcgc cgtcgcctcg ggtctccaga tggtggcgga cggggcggac
     1981 atcctggaca tcggcgggga gtccacgcgt cccggtgccg atcccgttgg cgccggggaa
     2041 gagatgcggc gcgtgcttcc cgtcatcgag cgactggcgg cgcagtgtcc tgcgctcatc
     2101 tccatcgaca cctacaaggg tgccgtcgcg cgtgaagcgg tcgcccgggg tgccagcatc
     2161 atcaacgaca tcagcggact ccagtacgat ccggacctcg cgcgtgccgc ggccgaggcc
     2221 ggcgcggggc tcgtcctgat gcacacgcgc ggacgttcgc gcgacatgta cgagcacgcg
     2281 gtttacgagg acgtggtgga ggaggtcgtg cgggagctcg acgcgtcgat cggccgcgcg
     2341 acggccgagg gcatccggcg cgacgccatc gttgtcgatc cgggcttcgg cttcgcgaag
     2401 cgcgcggagc atagctacga ggccctcgcg cggctcgatc ggctccgggt gctggaccgg
     2461 cccgttctct cgggcccgtc gcgaaagtcg ttcctgaagg cggc
//
LOCUS       DASTZR010000425         7308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_649966, whole genome shotgun sequence.
ACCESSION   DASTZR010000425 DASTZR010000000
VERSION     DASTZR010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7308
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649966"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VFV20_09410"
     CDS             complement(<1..218)
                     /locus_tag="VFV20_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288609.1"
                     /translation="MSRTARRNRGAVPTSGSRIRNEVAAIALVAFAVLSLVALFVDEN
                     AVLLHWWRGFLFSFLGWGALVVPLVLAAL"
     gene            complement(264..1928)
                     /locus_tag="VFV20_09415"
     CDS             complement(264..1928)
                     /locus_tag="VFV20_09415"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU5288610.1"
                     /translation="MSSALRVIPLGGVGEIGKNMLALEQGDDIVVIDAGLMFPDEEML
                     GIDLVIPDIAYLRQRKDKVRGIVLTHAHEDHMGALPYVLRDFIHVPVYGTKLTLGLMR
                     TKLREHKLHEKVDAREIAPGTPFRVGAFTIDSYAVCHSIPDAVGITIETAQGTVVHSG
                     DWKFDHTPVDGRQTDFARLSEIAAKGVLLLMSDSTRAEVPGYTPSERHVGELLDTIFA
                     RAQGRVITTTFASNISRIKQIIEIAAFWGRRTAIVGRSMDNYTRTARELGYLALPEGS
                     LIQPKEIDKLADNELCIITTGSQGEPTSALSRMALGDHRHVAVKDGDTVVMSATPIPG
                     NEELVSRTVDNLFKLGAEVIYDPGVRPHVSGHASQEELKLLLNILRPKYFVPIHGEYR
                     MLVRHARLAIDLGVQPENAFVITNGDVLELSDGRAKIGEKVAAGVVYVDGLGVGDVSQ
                     SVMRDRWSIGSDGIFLVVLTIDRQTGQVVAGPDIVTKGFVPEEDAADLVERTRVRVLD
                     GLGETQSGEHLAEVATLRDTIHDIVSSYLYERTKRRPMVLPVIMQV"
     gene            complement(1921..2541)
                     /locus_tag="VFV20_09420"
     CDS             complement(1921..2541)
                     /locus_tag="VFV20_09420"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU5288611.1"
                     /translation="MIDEQLRALAAEIRACTACPLHRSARQAVPGEGSAESGIFFLGE
                     APGYNEDVQGRPFVGAAGQLLDELLRGIGLDRSKVFITNVVRHRPPENRDPLPDEVTA
                     CDVWLRRHLALLQPKVIVTLGRHAMYKFFPGESISRIHGRPRNQEGLVIFPIYHPAAA
                     LHQPALRDALAADFTALALLLASAPAAARPAEAAAKPADQMTLFSE"
     gene            complement(2564..3238)
                     /locus_tag="VFV20_09425"
     CDS             complement(2564..3238)
                     /locus_tag="VFV20_09425"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5288612.1"
                     /translation="MPGSTATGALGRAVARYGKAPLGARLFVHGRAFLSDLAMVERYV
                     PKKGFVVDLGCGHGLFACVLRESSPTRRVLGIDLDPRKIEVARSAIQDTQWLRFEVGD
                     IVASAPPKCDAVTIVDVLYLLPYETQEQVLRNAASALGEGGPLVVKAQERRVDPRYAL
                     TYAQELVTVSLGFTRGGRERFYFPSREEALAMFDRAGFVVDVVEMPRRPYTDVVYLAR
                     KAPTRS"
     gene            complement(3261..4055)
                     /locus_tag="VFV20_09430"
     CDS             complement(3261..4055)
                     /locus_tag="VFV20_09430"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEU5288613.1"
                     /translation="MTLVVTADDLGLSDGVTRGILEAHRAGVVRSTSLLVTFPSSEEA
                     AARALAEPDLEVGIHLDLVGGRPVSDPAAVRSLCDAEGRFHPVEDLTGRLFTGRIRVD
                     EVATELRAQVDRARSWGVPALAWDSHRHVHLMPPLARVFSALAREKGAAWVRRGRAPR
                     LSLMPKQMALHASSLVSELAYRRLPGNSWFIDLTAARPRFDATTVALLATLAGVGELA
                     AHPGYADDELRRTDTLVDGREDDLVLLTDPLLREALGHDCVVWRVR"
     gene            complement(4063..4794)
                     /locus_tag="VFV20_09435"
     CDS             complement(4063..4794)
                     /locus_tag="VFV20_09435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5288614.1"
                     /translation="MLSLILPAYNEARRLPASLAACAEYFVGQGLRAEIIVADDGSTD
                     ATPKVFADAVATLPRAGLDIRYLSLPHRGKGSAVRAGVLAAKGDPIAFLDSDLTIPVE
                     ILERFARALDDGADIAVASRYVHGSVVKRPWWRSLLGVVFRRCVHLLVPVDVSDTQCG
                     GKMYTAEAAKDLYRRSLLDGFTFDAEVLYLARRAGYRVKEIPFRLVQDRVTSIDFLAE
                     TPRMLRDLFLIRANALLGRYGRSRS"
     gene            complement(4798..7023)
                     /locus_tag="VFV20_09440"
     CDS             complement(4798..7023)
                     /locus_tag="VFV20_09440"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU5288615.1"
                     /translation="MAVTQASATINGKPLTFETGRFAKQAGGAVTVRYGDTIVFAAGT
                     VAKTTRPGIDFFPLTVDFEERLYAAGKIPGSFPRREGRPSEEGILTARLVDRPIRPLW
                     PKGFKNDVQIIVSAWSSDQENDYDVLAVNAASAALHISNAPFEGPIGCVRVGTVDGKL
                     TLNPTITQMLESELDLVVAGTRDSIMMIEAGANQVSEDTLVEALRLAHEGIKELCAAQ
                     ETLRQSVGKPKLEFAAPAVDTAKLAPVRTFLGERLRAKLRNPDKAQREAGLDELKNEA
                     VAKFAVEEGSLTTADEVGAVYEEVLEEEFRRAVTDENLRPDGRGTKDIRPLSIEVGVL
                     PRTHGSGLFTRGQTQVLSIATLGPTGDEQIIDTLSPVDTKRYMHHYNFPPYSVGEVRM
                     MRGAGRREIGHGALAERALVPVLPTKEEFPYTIRVVSETLESNGSSSMASTCGSTLAL
                     MDAGVPIKEMIGGIAMGLVTHGDKFTILTDIQGLEDHYGDMDFKVTGSTKGVTALQMD
                     IKIKGISIDVMAQAFAQAREARLFILDAMRRVIERPREDLSKYAPRIETIKIHPDKIR
                     EVIGPGGKMIRSIQSQTGTNIELEDDGTVRITGAKPEGREQAKLMILGMTREPEVGEV
                     FDGKVTRIMSIGAFVEYLPGKEGLVRASEISTERVNRIEDAVKIGDTVKVKIAEVDRQ
                     GRVNLSIRAVTEEGNGYEERQRAERARFQDREGGDRGGFRRGPGGGGGGFRRSGPPPR
                     G"
     gene            complement(7098..>7308)
                     /gene="rpsO"
                     /locus_tag="VFV20_09445"
     CDS             complement(7098..>7308)
                     /gene="rpsO"
                     /locus_tag="VFV20_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008888875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU5288616.1"
                     /translation="DSGSPEVQIALLTDRIKHLTEHLRTARHDNSSRRGLLMMVGQRR
                     RLLAYLHQKSPERYQAIIGRLGLRR"
BASE COUNT         1105 a         2492 c         2546 g         1165 t
ORIGIN      
        1 agcgccgcga ggacgagcgg cacgaccagg gcgccccagc cgaggaacga gaacaggaag
       61 ccgcgccacc agtgcaggag caccgcgttc tcgtcgacga agagcgcgac gagggacagc
      121 acggcgaacg ccacgagcgc gatcgcggcg acctcgttgc ggatgcgcga tccactcgtg
      181 gggaccgctc cgcggttcct cctcgctgtc cggctcaatc gctgctcaat ctctggcctc
      241 tggccaggag atacgcctca caactagacc tgcatgatca cgggcagcac catcggacgg
      301 cgcttggtgc gctcgtagag gtacgacgac acgatgtcgt ggatcgtgtc gcgcagcgtg
      361 gcgacctcgg cgaggtgctc gccgctctgc gtctcgccga ggccgtcgag cacgcgcacg
      421 cgcgtccgct cgacgaggtc cgcggcgtcc tcctcgggca cgaagccctt cgtcacgatg
      481 tccggcccgg cgacgacctg gccggtctgg cggtcgatgg tcaggacgac caggaagatg
      541 ccgtcgcttc cgatggacca gcggtcacgc atgaccgact ggctcacgtc ccccaccccc
      601 aggccgtcca cgtacaccac gcccgccgcc accttctcgc cgatcttcgc ccgtccatca
      661 ctcaactcca gtacgtcgcc gttcgtgatg acgaaggcgt tctcgggctg gacaccgagg
      721 tcgatcgcga ggcgggcgtg gcggacgagc atgcggtact cgccgtggat cgggacgaag
      781 tacttgggtc gcaggatgtt gaggagcagc ttcagctcct cctggctcgc gtgaccggag
      841 acgtgcggcc ggacgccggg gtcgtagatg acctcggcgc ccagcttgaa gaggttgtcc
      901 acggtgcgtg acaccagttc ctcgttgccc gggatcggcg tcgcggacat gacgacggtg
      961 tccccgtcct tcaccgcgac atgccgatgg tcgccgaggg ccatccgcga gagcgcgctc
     1021 gtcggctcgc cctggcttcc ggtggtgatg atgcagagct cgttgtcggc gagcttgtcg
     1081 atctccttcg gctggatgag cgacccctcg ggcagcgcga ggtagccgag ctcgcgcgcc
     1141 gtcctcgtgt agttgtccat cgagcgcccc acgatcgcgg tgcggcggcc ccagaacgcg
     1201 gcgatctcga tgatctgctt gatccgcgag atgttcgacg cgaaggtcgt cgtgatcacg
     1261 cgaccctgcg cgcgagcgaa gatcgtgtcg agcagctcgc cgacgtgacg ctcggacggc
     1321 gtgtagccgg ggacctccgc gcgggtcgag tccgacatga ggagcagcac gcctttcgcg
     1381 gcgatctccg acaggcgcgc gaagtcggtc tgccgtccgt cgacgggcgt gtggtcgaac
     1441 ttccagtcgc cggagtggac caccgtgccc tgcgcggtct cgatggtgat gcccaccgcg
     1501 tcggggatgg agtggcacac cgcgtacgag tcgatcgtga aggcgccgac ccggaacggc
     1561 gtacccgggg cgatctcacg cgcatcgacc ttctcgtgca gcttgtgctc gcgaagcttc
     1621 gtgcgcatga gcccgagcgt gagcttggtg ccgtacaccg ggacgtggat gaaatcgcgg
     1681 aggacgtacg ggagcgcacc catgtggtcc tcgtgcgcgt gcgtcagcac gatgccgcgg
     1741 accttgtcct tccgctggcg caagtacgcg atgtcgggga tgacgagatc gatgccgagc
     1801 atctcctcgt cggggaacat gaggccggcg tcgatgacga cgatgtcgtc gccctgctcg
     1861 agcgcgagca tgttcttgcc gatctccccg acgccgccga gcgggatgac ccgaagcgcg
     1921 ctactcactg aacaacgtca tctggtccgc gggcttggcc gccgcctccg cgggccgcgc
     1981 cgccgccggt gcgctggcga gcagcagcgc gagcgccgtg aagtccgcgg cgagcgcatc
     2041 gcggagcgct ggctggtgca gcgcggcggc gggatggtag atcgggaaga tcacgaggcc
     2101 ctcctggttg cgtggccggc catggatgcg tgagatcgat tcgcccggga agaacttgta
     2161 catcgcgtgc cgtcccagcg tcacgatcac ctttggctgc agcagcgcca ggtggcgacg
     2221 cagccacacg tcgcatgcgg tgacctcgtc gggcaacggg tcgcggttct ccggcggacg
     2281 gtggcggacg acgttggtga tgaagacctt cgagcgatcg aggccgatgc cgcgcagcag
     2341 ctcgtcgagc agctgtcccg ccgcgcccac gaacggtcgg ccctggacgt cctcgttgta
     2401 tccgggggcc tcgccgagga agaagatgcc cgactcggcg cttccctcgc cggggacggc
     2461 ctgtcgcgcg ctgcggtgga gggggcaggc ggtgcaggcg cggatctctg cggccagagc
     2521 gcgcagctgc tcatcgatca tccgagcgaa ttatcgctcc cgctcaggat cgcgtcggtg
     2581 ccttcctcgc cagatacacc acatccgtgt acggacggcg gggcatctcg acgacgtcca
     2641 cgacgaatcc ggcacggtcg aacatcgcga gcgcctcctc acgcgacggg aaatagaagc
     2701 gttcgcggcc cccgcgagtg aatccgaggc tcaccgtgac cagctcctgc gcgtaggtga
     2761 gcgcgtagcg ggggtcgacc cgccgctcct gggccttcac caccagcggc ccgccctcgc
     2821 ccagggcgct ggcggcgttc cggagcacct gttcctgggt ctcgtacggc aggaggtaga
     2881 ggacgtccac gatcgtgacc gcgtcgcact tgggcggggc cgacgccacg atgtccccca
     2941 cctcgaaacg cagccactgg gtgtcctgga tcgcgctgcg cgcgacctcg atcttgcgtg
     3001 gatcgaggtc gatgccgagc acccggcgcg ttggggagga ctcccgcagg acacaggcga
     3061 acagcccgtg gccgcatcca aggtccacga cgaagccctt cttgggcaca taccgctcga
     3121 ccatcgccag gtccgacagg aacgcgcggc cgtgcacgaa aagacgcgct ccaagcggcg
     3181 ccttgccgta gcgcgcgacc gcccgcccga gggcgccggt ggcggtgcta cccggcacta
     3241 gacgatgacc cgagtgtcag tcaacgcact ctccatacga cgcagtcgtg gcccagggcc
     3301 tcgcgcagca gcgggtcggt cagcaggacg aggtcatcct cgcggccgtc gacgagcgta
     3361 tcggtgcggc gcagctcgtc gtcggcgtag ccgggatgcg cggcgagctc gcccaccccc
     3421 gcgagcgtgg ccagcagcgc caccgtcgtt gcgtcgaagc gcgggcgggc cgcggtgagg
     3481 tcgatgaacc aggagttccc gggcagccgc cgataggcca gctcggacac gagcgatgac
     3541 gcgtggaggg ccatctgctt cggcatgagc gagaggcgcg gcgcgcgccc gcggcgcacc
     3601 caggccgcgc ccttctcgcg ggcgagcgcg gagaagaccc gcgccagcgg cggcatgagg
     3661 tggacgtgac ggtgcgagtc ccacgcgagc gccggaacgc cccaggaccg cgcacggtcc
     3721 acctgggcac gcagctcggt ggcgacctcg tccacacgga tccggcccgt gaagaggcgg
     3781 ccggtgaggt cctccacggg atggaaccgg ccctccgcgt cgcacagcga ccgcacggcc
     3841 gcgggatcgc tcaccgggcg cccgccgacc agatcgaggt ggatgccgac ctccaggtcg
     3901 ggctccgcga gcgcccgcgc ggcggcctcc tcgctcgagg ggaacgtgac gagcaggctc
     3961 gtggaccgca ccacgccagc ccggtgcgcc tcgagtatcc ctcgcgtcac gccgtcggag
     4021 aggccgaggt cgtccgcggt cacgacgagc gtcacgggca gatcacgatc gcgaccgacc
     4081 gtagcgcccg agcagcgcgt tcgcccggat caggaagagg tcgcgcagca tgcgcggcgt
     4141 ctccgcgagg aagtcgatgc tcgtgacgcg gtcctggacc agccggaagg ggatctcctt
     4201 gacgcggtaa ccggcgcggc gcgccaggta gaggacctcc gcgtcgaagg tgaatccgtc
     4261 cagcagcgag cgccggtaca ggtccttcgc cgcctcggcc gtgtacatct tcccgccgca
     4321 ctgcgtgtcg ctcacgtcca ccggaaccag cagatggaca cagcggcgga acacgacacc
     4381 caggaggctc cgccaccacg gccgcttcac gaccgaacca tggacatagc gcgacgcgac
     4441 cgcgatgtcg gcgccgtcgt ccagcgctcg ggcgaagcgc tccaggatct cgaccgggat
     4501 cgtcagatct gagtcgagga acgcgatggg atcgcccttg gccgcgagca cgcccgcccg
     4561 caccgccgac cccttcccgc gatgcggcag gctgaggtag cggatgtcga gcccggcgcg
     4621 cggcagcgtc gcgaccgcgt ccgcgaagac cttgggggtc gcatccgtcg aaccgtcgtc
     4681 ggcgacgatg atctcggcgc gcagtccctg cccgacgaag tactcggcgc atgcggcgag
     4741 gctcgccgga agccggcgag cctcgttgta ggcaggaagg atcagggaaa ggatcggtta
     4801 cccgcggggt ggtggaccgc ttcggcggaa gcctcccccg ccgcctccgg gaccccggcg
     4861 gaatccaccg cggtcgccgc cctcgcggtc ctggaagcga gcgcgctccg cgcgctggcg
     4921 ctcctcgtag ccgttgccct cttcggtcac ggcgcggatc gagaggttca cgcggccctg
     4981 ccggtccacc tccgcgatct tcaccttcac ggtgtcgccg atcttcacgg cgtcctcgat
     5041 gcggttcacg cgctcggtcg agatctccga ggcgcgcacg aggccctcct tgcccgggag
     5101 gtactcgacg aacgcgccga tgctcatgat ccgggtgacc ttgccgtcga acacctcgcc
     5161 gacctcgggc tctctggtca tgccgaggat catcagcttg gcctgctcgc gaccctccgg
     5221 cttcgcgccc gtgatgcgga cggtgccgtc gtcctcgagc tcgatgttcg tgcccgtctg
     5281 gctctggatg ctgcggatca tcttgccgcc ggggccgatg acctcgcgga tcttgtcggg
     5341 gtggatcttg atcgtctcga tgcgcggggc gtacttcgac aggtcctcgc gcgggcgctc
     5401 gatgaccctg cgcatcgcgt cgaggatgaa gagccgtgcc tcgcgcgcct gcgcgaaggc
     5461 ctgcgccatc acgtcgatgg agatgccctt gatcttgatg tccatctgca gggcggtgac
     5521 gcccttggtg gacccggtga ccttgaagtc catgtcgccg tagtggtcct cgaggccctg
     5581 gatgtcggtc aggatcgtga acttgtcgcc atgggtcacc agacccatcg cgatgccgcc
     5641 gatcatctcc ttgatcggca cgcccgcgtc catgagcgcg agcgtcgagc cgcacgtcga
     5701 cgccatcgac gacgagccgt tcgactcgag cgtctcggag acgacgcgga tggtgtacgg
     5761 gaactcctcc ttcgtcggga ggaccggcac gagggcgcgc tcggcgagcg caccgtggcc
     5821 gatctcgcgg cggccggcgc cgcgcatcat gcggacctct ccgaccgaat acggcgggaa
     5881 gttgtagtgg tgcatgtagc gcttcgtgtc caccggcgag agcgtgtcga tgatctgctc
     5941 gtcccccgtc gggccgagcg tggcgattga gagcacctgc gtctggccgc gcgtgaagag
     6001 cccggacccg tgggtgcgcg gaaggacgcc gacctcgatg gagagcggac ggatgtcctt
     6061 ggtgccgcgg ccatccggac ggaggttctc gtcggtgacc gcgcggcgga actcctcttc
     6121 gaggacctcc tcgtagaccg cgccgacctc atccgccgtg gtgaggctgc cctcttccac
     6181 ggcgaatttc gcgacggcct cgttcttcag ctcgtcgagc ccggcctcgc gctgtgcctt
     6241 gtcggggttc cggagcttgg cacgcagccg ctcgccgagg aacgtgcgca ccggggcgag
     6301 cttggcggtg tccaccgcgg gcgcggcgaa ctcgagcttc ggcttgccga cggactgacg
     6361 cagcgtttcc tgcgcggcgc agagctcctt gatgccttcg tgcgcgagcc gcagcgcttc
     6421 cacgagggtg tcctcgctca cctggttcgc gccggcctcg atcatcatga tcgagtcgcg
     6481 tgtcccggcg accacgaggt ccagctcgga ttccagcatc tgcgtgatcg tggggttcag
     6541 cgtgagcttg ccgtcgacgg tgcccacgcg gacgcatccg atcggaccct cgaagggcgc
     6601 gttcgagatg tggagcgcgg ccgacgccgc gttcaccgcg agcacgtcgt agtcgttctc
     6661 ctgatcgctc gaccaggccg acacgatgat ctgcacgtcg ttcttgaagc ccttcggcca
     6721 gagcggccgg atcgggcggt ccaccagacg agcggtgagg atgccctcct cggacggccg
     6781 tccctcgcga cgcgggaacg aaccggggat cttgcccgcg gcgtagaggc gctcctcgaa
     6841 gtcgacggtg agcgggaaga agtcgatccc ggggcgcgtg gtcttcgcga cggtgccggc
     6901 cgcgaacacg atcgtgtcgc cgtagcgcac ggtgaccgcg ccgccagcct gcttcgcgaa
     6961 tcggccggtc tcgaaggtga ggggcttccc gttgatggtc gcggaggctt gcgtgactgc
     7021 catgagtttg ttgttgtgga tgtcgcgcct atgaaaagac ccagggctgg ccctgggtca
     7081 tctctcccgc gggctcgtta ccgccgcaga ccgagtcgcc cgatgatcgc ctggtagcgc
     7141 tcggggctct tctgatgcag gtaggcgagc agccgccgtc gctgtccgac catcatgagc
     7201 agcccacgac ggctcgagtt gtcgtgacgg gccgtacgga ggtgctcggt caggtgcttg
     7261 atgcgatctg tgagcagtgc gatctggacc tctggagaac cgctgtcc
//
LOCUS       DASTZR010000426         4946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_23746, whole genome shotgun sequence.
ACCESSION   DASTZR010000426 DASTZR010000000
VERSION     DASTZR010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23746"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VFV20_09450"
     CDS             <1..444
                     /locus_tag="VFV20_09450"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU5288617.1"
                     /translation="AATTLTLGIVAASVTPPNASAAVTTSAPATASPEPSGLADLASR
                     VFAPLTESASSATPRPSASIAITKEAAESGATLFVIKGCVLCHGQRAEGQFGPKIART
                     VLPFEDVKRQARTPRNVYMPAFTQPLLSEEELVDIYVFLQTLPAE"
     gene            complement(445..1056)
                     /locus_tag="VFV20_09455"
     CDS             complement(445..1056)
                     /locus_tag="VFV20_09455"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU5288618.1"
                     /translation="MGAARGHGPAVARTTAVILAAGEARRFGAPKLLAPLEGRPLLQH
                     VIDAANASACDDVIVVVGHGAPEVLAKVRLGRAVAALNSAYANGQSTSLRAGLRRARG
                     SDAAVVLLGDQPRVTARLIDALLDRQRDTGASAVICARDGHRSPPTLLRRDLWPAIDA
                     LTGDVGARELLRDRDDVAVVEVTPDLGGLDDVDRPSDLDALGA"
     gene            1150..2208
                     /locus_tag="VFV20_09460"
     CDS             1150..2208
                     /locus_tag="VFV20_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288619.1"
                     /translation="MAIREDAAGAVTTLARESVVERAFVVVAGWLMTGVFLDAWAHIS
                     HLPDSFWTPWHGVLYSGLLACGAFLVLARSVERRIGRRLLAHGYGLSLAGFALGALGG
                     IADAIWHTVFGVEFDIDAAVSPSHLLIAGAILLVVTGPVRAAWSRRSFGLPAALGLLY
                     GISIITIIVDYAQPFGQLYGTGAPPAGRELGQLQQTVALFAFIAYAALLSGTILAALR
                     AGPIAPAWFGLVAGGNMAALTLVNGPLHPGATSLLLLVALASAALITAAAALLRPAPG
                     RIAATRTFAFAAPALSYAAYAVAVTAAVGTWWTPTFWCGLVAIGGLAGLLVSTLAAPS
                     AHTIDEPQTPRAEVRSLG"
     gene            2245..2739
                     /locus_tag="VFV20_09465"
     CDS             2245..2739
                     /locus_tag="VFV20_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5288620.1"
                     /translation="MPRITTFLTFNDQAEAAVKLYTGVFKNSRITSTTRYGEGAPLPA
                     GTLMSVEFELDGQPFMALNGGPSFAFSEGISLFVSCETQQEIDDHSAKLTAGGGQQGS
                     CGWLTDRFGVSWQIVPTILGSLIGDADPAKAQRAMQAMLGMKKLDIAALRRAHEGRST
                     ATAR"
     gene            2785..3114
                     /locus_tag="VFV20_09470"
     CDS             2785..3114
                     /locus_tag="VFV20_09470"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5288621.1"
                     /translation="MGSLDTARAFVKAVQDQKADAAADLCADDVEVMLPGADAPLRGK
                     EGVRQMIRLAPQFEHSMREEEERDGEVRITTLTRAPGVFANYTTWRFMLDGDLVKRLS
                     FDLKGAN"
     gene            3151..3927
                     /locus_tag="VFV20_09475"
     CDS             3151..3927
                     /locus_tag="VFV20_09475"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016696038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HEU5288622.1"
                     /translation="MTARIPAVYLGHGAPPLVDDPIWPGELSRWAGDLPKPKGILVVS
                     AHWEESPLTIGATTPVPLVYDFYGFPERYYQVTYGAPGAPWLAERVKQLIGAKTPVAE
                     EPTRGLDHGAYVPLLVMYPEADIPVLQISMPSLDARELLELGRKLAPLRDEGVLIMGS
                     GFMTHGLPFLRDFRPDAPAPSWSKEFDHWAAETIAKADVDALLDYRRRAPGLPYAHPT
                     VDHFVPLFVTVGASLDQIATGRTVIDGYFLGLSKRSLQFA"
     gene            3993..>4946
                     /locus_tag="VFV20_09480"
     CDS             3993..>4946
                     /locus_tag="VFV20_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288623.1"
                     /translation="MGVLARLVSSLLVISMLSIAGPLGRTAEAATCVGSTGPNVPPPA
                     VTSFPSGVPGFHAQWYGQSGYPTLCPGERSTATVAYYNSGSLGWVSGRLGEVAYLGTW
                     DPEPGQDRASPLGGDGTNGSPTTGWPRNNRIAIQPAPYVGPGQVAWFQFTIQAPSTPG
                     YYRLYLRPLIEGATWMEDFGVYWPVTVLNADGSRPPQPPAVYSPTGYSRLSVPTSAGT
                     FSASLIKERLDAVRVRTVTASTTDCTSACPAKPLDQYVSENGAYAGIHGSYFCPPDYA
                     PCAGIVNTYDYAVYNSNLGRWINARHLSNPTNALAVWSGSTP"
BASE COUNT          696 a         1813 c         1695 g          742 t
ORIGIN      
        1 gccgcgacga ccctcacgct cgggatcgtc gcggcgagcg ttaccccgcc gaacgcaagc
       61 gcggccgtga cgaccagcgc gcccgccacg gcctctcccg agccgagcgg cctcgcggac
      121 ctcgcgagcc gcgtcttcgc cccgctcacc gagtccgcgt cgtcggccac accacgtccg
      181 tccgcgtcca tcgcgatcac gaaggaggcg gccgagagcg gggcgacgct cttcgtcatc
      241 aagggctgcg tcctgtgtca cggtcagcgc gccgagggac agttcggacc gaagatcgcg
      301 cgcacggtcc tcccgttcga ggacgtgaag cgccaggcgc gcacgccgcg caacgtgtac
      361 atgccggcgt tcacgcagcc gctcctctcc gaagaggagc tcgtcgacat ctacgtcttc
      421 ctgcagaccc tccccgcgga gtgactacgc gccgagcgcg tccaggtcgc tcggccgatc
      481 gacgtcgtcg agaccgccca gatcgggcgt gacctcgacg acggccacgt cgtcacgatc
      541 gcgcagcagc tcccgcgctc cgacgtcacc ggtgagcgcg tcgatggccg gccagagatc
      601 gcggcgcagg agcgtgggcg gggagcgatg accgtcgcgc gcgcagatca cggcgctcgc
      661 acccgtgtcg cgctgacggt cgaggagcgc atcgatgagg cgcgcggtga cacggggctg
      721 atcgccgagc agcacgacgg ccgcgtccga tccgcgcgcg cggcggaggc ccgcgcgcag
      781 cgacgtcgat tgcccgttcg cgtacgcgct gttgagcgcc gcgaccgcgc gcccgagccg
      841 caccttggcg aggacctccg gcgcgccgtg gccgacgacg acgatcacgt cgtcgcaggc
      901 cgacgcgttc gcggcgtcga tcacgtgctg gaggagaggc cgtccttcga gcggcgcgag
      961 cagcttcggc gcgccgaacc gcctcgcctc gccggccgcg aggatcaccg cggtcgttcg
     1021 cgcgacggca ggaccgtgcc ccctcgcggc gcccatgagg gcattgggcc gccggcgccg
     1081 cggcccggca agggccgaag gtcccgtcgg gcgggcgagc gtgccgggcg ctcccggtac
     1141 ccttgagcga tggcgattcg cgaggatgcc gccggggcgg tcacgacgct cgcgcgcgag
     1201 tccgtcgtcg agcgagcgtt cgtcgtcgtc gcgggctggc tcatgaccgg cgtgttcctc
     1261 gacgcgtggg cgcacatctc tcatcttccc gactccttct ggacgccgtg gcacggggtg
     1321 ctctactccg ggctgctcgc atgcggcgcg ttcctcgtcc tggcgcggtc tgtggagcgc
     1381 cgcatcggac ggcgtctgct cgcccacgga tacggcctct cgctcgcggg cttcgcgctc
     1441 ggggcgctcg gcgggatcgc cgacgccatc tggcacacgg tgttcggcgt cgagttcgac
     1501 atcgacgcgg cggtgagccc gtcgcatctg ctgatcgccg gggcgatcct cctcgtcgtg
     1561 accggacccg tgcgcgccgc gtggtcgcgc cgctccttcg ggctgcccgc cgcgctcggc
     1621 ctgctctatg gcatctcgat catcacgatc atcgtcgact acgcgcagcc gttcggtcag
     1681 ctctacggca ccggcgcgcc tcccgccggc cgcgagctcg gccagctcca gcagaccgtc
     1741 gcgctcttcg cgttcatcgc ctacgcggcg ctgctcagcg gcacgatcct cgcggcgctt
     1801 cgcgcgggcc cgatcgcgcc ggcgtggttc gggctcgtcg cgggcggcaa catggccgcg
     1861 ctcactctgg tcaacggccc gctccatccg ggcgcgacct cgctgctgct cctcgtcgcg
     1921 ctcgcgagcg ccgcgctcat caccgccgcc gcagcgctcc tccggcccgc gccgggccgc
     1981 atcgcggcga cgcgcacctt cgcgttcgcg gcgcccgccc tgtcgtacgc cgcgtatgcc
     2041 gtggcggtga ccgccgccgt cgggacgtgg tggacgccga cgttctggtg cggactcgtc
     2101 gcgatcggcg gcctcgcggg cctcctcgtc tcgaccctcg ccgcgccctc cgctcacacg
     2161 atcgacgagc cgcagacccc gcgtgccgag gtacggagcc tcggctgagc gcggggcata
     2221 tcagaggaca cggaggtagg acgcatgccg aggatcacga cgttcctgac gttcaacgac
     2281 caggcggagg cggccgtcaa gctgtacacg ggcgtcttca agaactcacg gatcacgagc
     2341 accaccaggt acggcgaggg cgcacccttg ccggcgggca cgctgatgtc ggtggagttc
     2401 gagctggacg gacagccctt catggccctc aacggcggac cctcgttcgc cttcagcgag
     2461 gggatctcgc tcttcgtctc gtgtgagacc cagcaggaga tcgacgacca cagcgcgaag
     2521 ctgacggccg gcggcggcca gcagggatcc tgcggctggc tcacggaccg cttcggcgtg
     2581 tcctggcaga tcgtgccgac gatcctcggt tcgctcatcg gcgacgccga tccggccaag
     2641 gcgcagcgag cgatgcaggc gatgctcggc atgaagaagc tcgacatcgc cgcgctccgc
     2701 agggcgcacg aagggcgttc gaccgcgacg gcacggtgac gtgagcgtcg cgccgaacga
     2761 gaagaggaac gtaccatcaa agacatgggc tctctcgaca ccgcgcgcgc gttcgtcaag
     2821 gccgtccagg accagaaggc cgatgcggcc gcggatctct gcgcggatga cgtcgaggtg
     2881 atgcttcccg gcgccgacgc gccgcttcgc ggcaaggagg gcgtgcgcca gatgatccgc
     2941 ctcgcgccgc agttcgagca cagcatgcgc gaggaggagg agcgcgacgg cgaggtgcgc
     3001 atcacgacgc tcacgcgcgc gccgggcgtc ttcgcgaact acaccacctg gcgtttcatg
     3061 ctggatggcg acctcgtgaa gcggctctcc ttcgatctca aaggcgcgaa ctgacggaat
     3121 agcctcggcg ttcggagcgt tgaccgcgac gtgaccgctc gcatccccgc cgtctatctc
     3181 ggacacggcg caccgccgct cgtcgacgac ccgatctggc cgggcgagct ctcacgctgg
     3241 gccggcgacc tgccgaagcc gaagggcatc ctcgtcgtgt cggcgcactg ggaggagtct
     3301 cctctcacca tcggtgcgac gacgccggtg ccgctggtct acgacttcta cggatttccc
     3361 gagcgctact accaggtcac ctacggggcg cccggcgcgc cgtggctcgc cgagcgcgtg
     3421 aagcagctca tcggcgcgaa gacgccggtc gccgaggagc ccacgcgcgg gttggatcac
     3481 ggcgcgtacg tgcccctcct cgtgatgtac cccgaggcgg acattcccgt cctccagatc
     3541 agcatgccga gcctcgacgc gcgcgagctg ctcgagctgg gtcggaagct cgcgccgctg
     3601 cgcgacgaag gcgtgctcat catgggctcg ggcttcatga cgcacggact gccgttcctg
     3661 cgcgacttcc gtcccgacgc tcccgcgccg tcgtggtcga aggaattcga tcactgggcg
     3721 gccgagacga tcgcgaaggc ggatgtcgac gcgctcctgg actatcgccg tcgcgcaccg
     3781 ggccttccgt acgcgcatcc gacggtggat cacttcgtgc ccctcttcgt gacggtcggc
     3841 gcgagcctcg accagatcgc cacgggaagg accgtcatcg acgggtactt cctcggcctc
     3901 tcgaagcgct cactgcagtt cgcgtgagac cctgccggta gcaggccggt aacaccgtat
     3961 ttcggatggc gccgcggcgt tagcacggtc ggatgggtgt gctcgcgcgt ctggtctcct
     4021 ccctgctcgt gatcagcatg ctgtcgatcg cgggcccgct cggccgcacc gcggaggcgg
     4081 cgacgtgcgt cggcagcacg ggacccaacg tcccgccgcc cgcggtcacg agcttcccat
     4141 ccggcgtgcc gggcttccac gcgcagtggt acgggcagag cggctacccg acgctctgtc
     4201 ccggcgagcg ctcgaccgcg accgtcgcct actacaacag cgggagcctc gggtgggtga
     4261 gcggcaggct cggcgaggtc gcgtacctcg gcacctggga tcccgagccc ggccaggatc
     4321 gcgcgagccc gctcggcggc gacggcacga atggatcgcc cacgaccggt tggcctcgca
     4381 acaaccggat cgcgatccag cccgcgccgt acgtcggacc cggacaggtc gcgtggttcc
     4441 aattcacgat ccaggcgccg tccacgccgg gctactaccg cctctacctg cgtccgctca
     4501 tcgagggcgc gacctggatg gaggacttcg gcgtgtactg gccggtcacc gtgctgaacg
     4561 ccgacgggtc gcggccgccg cagcctccgg ccgtgtacag ccccaccggc tacagccgcc
     4621 tctcggtgcc gacgtcggcc ggcacgttct cggcctctct catcaaggag cggctggacg
     4681 cggtgcgcgt ccgcacggtc accgcatcca cgacggattg caccagcgcg tgtcccgcga
     4741 agccgctcga tcagtacgtg tcggagaacg gtgcgtacgc gggtatccac ggcagctact
     4801 tctgtccgcc ggactacgcg ccgtgcgcgg gcatcgtgaa cacctacgac tacgccgtgt
     4861 acaactcgaa cctcggccgg tggatcaacg cgcggcatct ctccaatccg acgaacgcgc
     4921 tcgcggtgtg gagcggcagc accccg
//
LOCUS       DASTZR010000427         3932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_963719, whole genome shotgun sequence.
ACCESSION   DASTZR010000427 DASTZR010000000
VERSION     DASTZR010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963719"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="VFV20_09485"
     CDS             complement(<1..971)
                     /locus_tag="VFV20_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288624.1"
                     /translation="MKPLPRAVLLLVAATVAAAVPFVLVGTIDVASGRTRLDIVLPLL
                     LLAFVLSLRPIRVQLNTELSPSDVAVLVGIVLLPPGAVAVVAGGGRLLTDVATRKRPV
                     QIVRNTAAVVVATGFAGMAYRAALAQTLPLADFAASAILSGVVAITTLVVLDIAQILL
                     LQRALGNMAFDRAAAQWIGRTARAQLLWSLAALITLQVVLIEPWFLVPAVPLFLMGYL
                     DIRARFAAERRARLLATLVEIGHAVGMSLDPTTVFREVFAQVRKALDVDAFYVAIADH
                     DRGVFSFRYLYENGHEMEPDEQAIEGTLAGICIERDRPLLLRDAERDR"
     gene            complement(1033..1161)
                     /locus_tag="VFV20_09490"
     CDS             complement(1033..1161)
                     /locus_tag="VFV20_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288625.1"
                     /translation="MAVLLVVFVIQPAMARGGFGELLIIAGIFLAILIVERWLRTR"
     gene            complement(1200..1985)
                     /locus_tag="VFV20_09495"
     CDS             complement(1200..1985)
                     /locus_tag="VFV20_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEU5288626.1"
                     /translation="MGDVTMKIFRGDRSGGELKTYTVPSDPGMVVLDVVHRVQATQEP
                     DLAVRWNCKAGKCGSCSAEVNGKPRLMCMTRMDTFEPGEDITITPLRTFPVIRDLVTD
                     VSYNYEKAKTIPGFKPKPKEKDGTRRMYQEDVDRVQEFHKCIECFLCQDVCHVIRDHE
                     ENKQSFAGPRYFVRIASLEMHPLDTNDRVELMRLKHGLGYCNITKCCTEVCPEGIRIT
                     DNAIIPLKERVVTRTYDPLGWALRRIRGQQTSLGAGEPEPPSA"
     gene            complement(1988..3823)
                     /locus_tag="VFV20_09500"
     CDS             complement(1988..3823)
                     /locus_tag="VFV20_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019872798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HEU5288627.1"
                     /translation="MAEPQRHEYDVIVIGAGGAGLRAAIEASAMGVRTALVCKSLLGK
                     AHTVMAEGGIAAALGNVWKEDGWKVHFRDTMRGGKLLNNWRMAQIHAQEAPDRVLELE
                     EWGALFDRTKDGLILQRDFGGHRYARLAHVGDRTGLEMIKTLQHHAVHKGIDVFMETT
                     VFTLLKEDGRVAGAFGYERQTGRPVLFHAKAVVLATGGIGKAWKVTSNSWEYTGDGIA
                     MALGAGAELLNMEMTQFHPTGMVWPLSVRGLLVTEGVRGDGGTLKNNKGERFMFKYIP
                     EFFKAETATTEEEADAWYTDKTKRRTPDLLPRDEVARAINAEVKAGRGTEHGGVYLDI
                     ASRRSADYIKKRLPSMYHQFMQLAGVDITKEPMEIAPTYHYLMGGIKVDADSQQTTVP
                     GLFAAGECAAGLHGANRLGGNSLSDLVVFGRRAGLYAAEYAKRLARMPLLDEEQVQRA
                     ERDLAAPLERHEGENPYAIQSELQQTMHELVGIIRTRAELEQAVVRIDALAMRAERAA
                     TSGGRTYNPSWHTALDIRSQMACAAVVARSALTREESRGGHTREDFPKADAAWGKKTI
                     VAKMRGGAIDISTEPVPEVPADLQAILGEDKPAGTAAVGATAGGV"
BASE COUNT          575 a         1331 c         1395 g          631 t
ORIGIN      
        1 cggtcccgtt cggcatccct cagcaggagc ggccgatcgc gctcgatgca gatgcccgcg
       61 agggtgccct cgatggcctg ttcgtcgggc tccatctcgt gtccgttctc gtagaggtac
      121 cggaacgaga acacgccgcg atcgtgatcg gcgatggcga cgtagaacgc atcgacgtcg
      181 agcgctttcc gcacctgcgc gaacacctca cggaagaccg tcgtcggatc gagggacatc
      241 ccgacggcgt gaccgatctc cacgagggtg gccagcaggc gcgcgcgccg ctcggccgcg
      301 aaccgcgcgc ggatgtcgag gtaccccatg aggaagagcg gcaccgccgg caccaggaac
      361 cagggctcga tgaggacgac ctgcagcgtg atgagcgccg cgaggctcca gagcagctgc
      421 gcgcgcgcgg tgcggccgat ccactgtgcc gcggcgcgat cgaatgccat gttcccgagc
      481 gcccgctgca gcagcaggat ctgcgcgatg tcgaggacga cgagcgtcgt gatggcgacg
      541 acccctgaca ggatcgcgct cgccgcgaag tccgcgagcg gcaatgtctg cgcgagagcc
      601 gcgcgatagg ccataccggc gaagccggtg gcgacgacga ccgcggcggt gttgcgcacg
      661 atctgcaccg gtcgcttgcg cgtcgcgacg tcggtgagca gacgcccgcc accggccacg
      721 acggccacgg cgccgggcgg caggagcacg atgcccacga gcacggcgac gtcggagggg
      781 ctgagctcgg tgttcagctg tacccgtatg ggtcgcaggg agaggacgaa cgcgaggagg
      841 agcagcggca gcacgatgtc gagccgcgtg cgtccgctcg ccacgtcgat cgtccccacc
      901 aggacgaacg ggacggcggc tgcgacggtc gcggcgacga gcaggaggac ggcacgcggt
      961 agtggcttca cagacgcccc tcgggcggcg agcctagcac ccacccgacg gcggaccggc
     1021 gtcagccggg actcagcgcg tccggagcca ccgctccacg atgaggatcg cgaggaagat
     1081 gccggcgatg atcagaagct cgccgaagcc gccgcgcgcc atggccggct ggatgacgaa
     1141 gacgaccagc agcacggcca tgaggacgcc gacgaggatc tggaacgcga gccggcttct
     1201 caggcggagg gtggctctgg ttctcccgct ccaagggacg tctgctgtcc gcggatccgg
     1261 cggagcgccc agccgagcgg gtcataggtg cgcgtcacga cgcgctcctt cagcgggatg
     1321 atcgcgttgt cggtgatgcg gatgccctcg gggcatacct cggtgcagca cttggtgatg
     1381 ttgcagtagc cgaggccgtg cttcagccgc atgagctcca cgcggtcgtt ggtgtcgagc
     1441 ggatgcatct cgagcgacgc gatgcgcacg aagtagcgcg ggcctgcgaa gctctgcttg
     1501 ttctcctcgt ggtcgcggat gacgtggcag acgtcctggc acaggaagca ctcgatgcac
     1561 ttgtggaatt cctgtacgcg gtcgacgtcc tcctggtaca tccgccgcgt cccgtccttc
     1621 tccttgggct tcggcttgaa ccccgggatg gtcttcgcct tctcgtagtt gtacgagacg
     1681 tcggtgacga ggtcgcggat caccgggaac gtccggagcg gcgtgatggt gatgtcctcg
     1741 ccgggctcga aggtgtccat gcgggtcatg cacatgaggc gcggcttgcc gttcacctcg
     1801 gcgctgcagg agccgcactt gccggccttg cagttccagc gcacggcgag gtcgggctcc
     1861 tgcgtcgcct gcacccggtg gacgacgtct aggaccacca tgccgggatc gctcggcacg
     1921 gtgtacgtct tcagctcgcc gccgctgcgg tccccgcgga agatcttcat cgtcacgtcg
     1981 cccacgtcta aacgcctcct gcggtcgcgc ccacggcggc ggtgcccgcc ggcttgtcct
     2041 cgccgaggat cgcctggagg tcggccggca cctccggcac gggctcggtc gagatgtcga
     2101 tcgcgccgcc ccgcatcttc gcgacgatcg tcttcttgcc ccacgcggcg tcggccttgg
     2161 ggaagtcctc gcgggtgtgg ccgccgcggc tctcctcgcg cgtgagcgcg gagcgggcga
     2221 cgacggcagc gcacgccatc tgcgatcgga tgtcgagggc ggtgtgccag gacgggttgt
     2281 acgtgcgccc gccgctggtc gccgcgcgct ccgcgcgcat ggcgagcgcg tcgatccgca
     2341 cgacggcctg ctcgagctcc gcacgcgtgc ggatgatgcc gacgagctcg tgcatcgtct
     2401 gctggagctc ggattggatc gcgtagggat tctcgccctc gtgccgctcg agcggggcag
     2461 ccaggtcgcg ctcggcacgc tgcacctgct cctcgtcgag gagcggcatg cgcgcaaggc
     2521 gcttcgcgta ctccgccgcg tagaggccgg cgcgccgtcc gaacacgacg aggtcggaca
     2581 gcgagtttcc gcccaggcgg ttcgcgccgt gcagcccggc cgcgcactcg cccgcggcga
     2641 agaggccggg cacggtcgtc tgctgcgaat cggcgtcgac cttgatcccg cccatgaggt
     2701 agtggtaggt gggcgcgatc tccatgggct ccttggtgat gtcgacgccg gcgagctgca
     2761 tgaactggtg gtacatcgag ggaagccgct tcttgatgta gtccgcggag cgccggctcg
     2821 cgatgtcgag gtacacgccg ccgtgctcgg tgccccgacc ggccttcacc tccgcgttga
     2881 tcgcgcgcgc gacctcgtcg cggggcagca ggtcgggcgt gcggcgcttc gtcttgtcgg
     2941 tgtaccaggc gtccgcctct tcctcggtcg tcgcggtctc ggctttgaag aactcgggga
     3001 tgtacttgaa catgaagcgc tcgcccttgt tgttcttcag cgtgccgccg tcgccgcgca
     3061 caccctcggt gacgagaagg ccgcggaccg acagcggcca gaccatgccc gtcgggtgga
     3121 actgggtcat ctccatgttg aggagctcgg cgccggcgcc gagggccatc gcgatgccgt
     3181 cgccggtgta ctcccacgag ttcgaggtga ctttccacgc cttgccgatg ccgcccgtcg
     3241 cgagcacgac ggccttcgcg tggaagagga cgggccggcc cgtctgtcgc tcgtagccga
     3301 acgcgccggc gacgcggccg tcctccttga gcagcgtgaa caccgtcgtc tccatgaaga
     3361 cgtcgatgcc cttgtgcacg gcgtggtgct ggagcgtctt gatcatctcg aggccggtgc
     3421 ggtcgccgac gtgggcgagc cgcgcgtagc ggtggccgcc gaagtcgcgc tggaggatga
     3481 ggccgtcctt ggtccggtcg aagagggcgc cccactcctc gagctcgagc acgcggtcgg
     3541 gcgcctcctg cgcgtggatc tgcgccatgc gccagttgtt cagcagcttg cccccgcgca
     3601 tcgtgtcgcg gaagtggacc ttccagccgt cctccttcca cacgttcccg agcgcggccg
     3661 cgatcccgcc ctcggccatg accgtgtggg ccttgccgag gagggacttg cagacgagcg
     3721 cggtgcgcac gcccatcgcg ctcgcctcga tcgcggcgcg cagtcccgcg ccgcccgcgc
     3781 cgatcacgat gacgtcgtac tcgtggcgtt gcggctcagc caccgaacac cacccgcggg
     3841 tcggtgatca cgccagcggc gagcaggcgg atgtacacgt cggtgatcac gacgaacgcg
     3901 aggctgaccc agaagaacgt tccgtgacgg ac
//
LOCUS       DASTZR010000428         5059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_546031, whole genome shotgun sequence.
ACCESSION   DASTZR010000428 DASTZR010000000
VERSION     DASTZR010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546031"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VFV20_09505"
     CDS             complement(<1..507)
                     /locus_tag="VFV20_09505"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU5288628.1"
                     /translation="MSASGTTPAAGGEQRRLGDRYLLLERIDAGGAGEVWRARDERLG
                     RDVALKILGADADEAFRGRFADEARRAAAVAHPNVVTVFDEGRDGEDSFMVMEFVRGK
                     TLRDMIAERGPLPAHEAARIVAQIAAALDAAHEAGVIHCDVKPANVILDEHGTAKLTD
                     FGIARAARG"
     gene            complement(546..1703)
                     /locus_tag="VFV20_09510"
     CDS             complement(546..1703)
                     /locus_tag="VFV20_09510"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HEU5288629.1"
                     /translation="MATALNAVDEAVWGAVVPNVGALERELTRLRRASSAHAREQGRS
                     VARAAVLNLVVYADREVHARRAAITTSRLANRHPSRGIIVLGDRDREGADVAVELHCH
                     VPRDDRAAQICYEQILARVRGDADDRIASLVIPLLVPDLPVFLWWTGTPPHDTRRFDD
                     LLSLADRLIVDSADFSRPDVTLPLLAELAMSQGYRSFGITDLNWTRLTPWRELVAGFF
                     DSAAWRPYLDEIVGVRIGFGVDMDGRDIHPSQVLLFVAWLASRLGWTPREALAPSEAG
                     GLLFRMSRADGAPIVVRMRPRFEQGIDPGDTSGVRIQARAGGQLAEFVLKRAPDARHV
                     TATVVLNGDTRATRVVPLPSWSIDELLREELVIVGSDRVYEAALHALVALA"
     gene            complement(1688..3220)
                     /gene="zwf"
                     /locus_tag="VFV20_09515"
     CDS             complement(1688..3220)
                     /gene="zwf"
                     /locus_tag="VFV20_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU5288630.1"
                     /translation="MSAVEQPTVVNPLREGLRLARTPEPATLVLFGGSGDLAARKLLP
                     ALYNLAVQRLLPASFAVVGAARRPSTDEAFRAELHAAVAEFSRTKPINEEVWSSFARN
                     MRFVATPDDAGYRALAARLAEVDAELGTNGNRLFYLATPPAAYAPIVRSIGTHGLRGP
                     GWSRVVIEKPFGHDLASATELTNIVHEVFREDEVFRIDHYLGKETVQNILVLRFANGI
                     FEPIWNRQYVDHVQITVAESIGIEERAEYYDRAGAMRDIVQNHLQQLLALVAMEPPAA
                     FDDRAVRDEKVKVLRALRPIAQREVAARTVRGQYVSGFIEGGRVPSYHEEAGIPPDSR
                     TETFVGMRCFVDNWRWEGIPFYLRTGKRLPKRATEIAIRFRVAPHRIFSRESSEGIDA
                     NELVLRIQPDEGISLTFGAKVPVQGFRIRTVNMDFVYGASFMVDAPDAYETLILDALR
                     GDATLFTRRDEVEQQWAFVDPILAAWRDGVPELCPYPAGSWGPPEGETLIERDGRVWR
                     RP"
     gene            complement(3240..4139)
                     /gene="gnd"
                     /locus_tag="VFV20_09520"
     CDS             complement(3240..4139)
                     /gene="gnd"
                     /locus_tag="VFV20_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEU5288631.1"
                     /translation="MDIGLYGLGRMGGNMVTRLLRGGHRVVAGNRSPGPVDEKKGEGA
                     VAAYTIEEMVRALTPPRVLWSMVPSGDATEAALDEFARHASKGDVLIDGGNSNFRDSM
                     RRAEKYAAQGLDFLDIGVSGGVWGLEVGYCMMVGGPERAVGLVRPALDTLAPPDGWSH
                     FGKSGAGHFVKMVHNGIEYGMMQAYGEGFELLHASEFEIDMKRVASVWMQGSVVRSWL
                     LELAVRAFEQEGTELANIKGWVADSGEGRWTIHEAIDHGVPMTALAHSLFARFTSRQD
                     DSYAMKVAAALRNQFGGHAVKTE"
     gene            4209..>5059
                     /locus_tag="VFV20_09525"
     CDS             4209..>5059
                     /locus_tag="VFV20_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013873057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU5288632.1"
                     /translation="MATATATPTRLDVYRQLARQLRADSIRCSTPVGSGHPTSSLSAA
                     DLMAVLLAGHLRYDWKKPDDPANDHLIFSKGHASPLVYALFRAAGAIDDAELMTFRRL
                     GSRLQGHPTPLLPWVDVATGSLGQGMPIAVGMALAAKRLWKAPFHIWTLVGDSESAEG
                     SVWEGFDLGGHESLENVTAILDVNRLGQRGPTELEWDTDAYAARIESFGWDALVIDGH
                     DLAEIDDALARARDASRPTAIVARTKKGRGYSKIEDQPGWHGKASSPEDAEEALRELG
                     GVRNDVIA"
BASE COUNT          744 a         1815 c         1764 g          736 t
ORIGIN      
        1 accgcgcgcc gcgcgcgcga tgccgaagtc ggtgagcttc gcggtgccgt gctcgtcgag
       61 gatgacgttc gccggtttca cgtcgcagtg gatgacgccg gcctcgtgcg ccgcatcgag
      121 tgcggccgcg atctgtgcca cgatgcgcgc ggcctcgtgc gccggcaggg gaccgcgttc
      181 ggcgatcatg tcgcgcagcg tcttgccgcg gacgaactcc atgaccatga acgagtcctc
      241 gccgtcgcgt ccctcatcga acaccgtgac gacgttcgga tgcgcgacag ccgccgcgcg
      301 gcgcgcttcg tccgcgaaac gcccgcggaa ggcctcgtcg gcgtccgcgc cgaggatctt
      361 gagggccacg tcgcggccga gccgctcgtc gcgggcgcgc cagacctctc cggcgccgcc
      421 cgcatcgatg cgctcgagca gcaggtagcg gtcgcccagt cggcgctgct cgccacccgc
      481 cgccggggtc gttccggacg cgctcaagcg tccctccatg ggcacgtccg ctcgtgcggc
      541 aagccctagg ccagcgcgac cagggcgtgc agcgcggcct cgtacacccg gtcgcttccg
      601 acgatgacga gctcctcccg caggagctcg tcgatgctcc aggacggcag cggcacgacg
      661 cgcgtcgccc gcgtgtcacc gttcagcacg accgtcgccg tgacgtggcg ggcgtccggc
      721 gcgcgcttca gcacgaactc ggcgagctgt ccgccggcgc gcgcctggat gcgcacgccc
      781 gacgtgtctc cggggtcgat gccctgctcg aaccgcgggc gcatgcgcac gacgatcggc
      841 gcgccgtccg cgcgcgacat gcggaacagg aggccgccgg cctcggatgg agcgagcgcc
      901 tcacgcggcg tccaccccag gcgcgacgcc agccacgcca cgaacagcag cacctgcgag
      961 gggtggatgt cgcgaccatc catgtcgacg ccgaagccga tacgcacgcc gacgatctcg
     1021 tcgaggtacg gccgccatgc cgcgctgtcg aagaatcccg cgacgagctc gcgccacggc
     1081 gtgaggcgcg tccagttgag gtcggtgatc ccgaacgagc ggtagccctg gctcatcgcg
     1141 agctcggcca gcagcggcag cgtcacatcg ggccgcgaga agtcggccga atcgacgatg
     1201 aggcggtccg cgagagacag caggtcgtcg aaacgccgcg tgtcatgcgg cggtgtgccg
     1261 gtccaccaca ggaacaccgg cagatccggc acgagcagcg ggatgacgag gctcgcgatg
     1321 cggtcgtcgg cgtctccgcg gacgcgcgcg aggatctgct cgtagcagat ctgcgcggcg
     1381 cgatcgtcgc gtggcacgtg gcagtggagc tcgacggcga cgtcagcacc ctcgcgatcg
     1441 cgatcgccga ggacgatgat accgcgcgag ggatgcctgt tggcgaggcg gctcgtggtg
     1501 atcgccgcgc ggcgcgcgtg gacctcgcgg tcggcgtaca cgacgaggtt cagcacggcg
     1561 gcccgcgcca cactccgtcc ctgctcacgt gcgtgcgcgc tcgacgcgcg acgcaggcgc
     1621 gtgagctccc gctcgagtgc ccccacgttc gggaccaccg cgccccacac cgcctcgtcg
     1681 accgcgttca gggccgtcgc cacaccctcc catcgcgctc gatcagcgtc tcgccttccg
     1741 gcgggcccca gctcccggcg ggatacggac acagctccgg caccccgtcg cgccacgcgg
     1801 ccaggatcgg atcgacgaac gcccactgct gctcgacctc gtcgcgcctg gtgaagagcg
     1861 tcgcgtcgcc gcgcagcgcg tcgaggatca gggtctcgta ggcgtcgggt gcgtccacca
     1921 tgaatgacgc gccgtacacg aagtccatgt tgacggtgcg gatgcggaac ccctgcaccg
     1981 ggaccttcgc gccgaaggta aggctgatgc cttcgtcggg ttggatgcgc agcacgagct
     2041 cgttcgcgtc gataccctcc gacgactccc gcgagaagat gcggtgcggc gcgacgcgga
     2101 agcggatcgc gatctccgtc gcgcgcttcg gaaggcgctt gccggtgcgg aggtagaacg
     2161 ggatcccctc ccagcgccag ttgtccacga agcagcgcat gcccacgaac gtctcggtcc
     2221 tggagtccgg cgggatgccg gcctcctcgt ggtagctcgg cacgcgcccg ccctcgatga
     2281 atccggacac gtactgcccg cggaccgtcc gtgcggcgac ctctcgctgc gcgatgggcc
     2341 ggagcgcgcg caggaccttg accttctcgt cgcggacggc gcggtcgtcg aacgcggcgg
     2401 gcggctccat cgcgacgagc gcgaggagct gctgcaggtg gttctgcacg atgtcgcgca
     2461 tcgcgccggc gcggtcgtag tactcggcgc gctcctcgat cccgatcgac tcggccacgg
     2521 tgatctgcac gtgatccacg tactggcggt tccagatcgg ctcgaagatc ccgttcgcga
     2581 accggagcac aaggatgttc tggacggtct ccttcccgag gtagtggtcg atccggaaca
     2641 cctcgtcctc gcggaacacc tcgtgcacga tgttcgtcag ctccgtcgcg ctcgcgagat
     2701 cgtggccgaa tggcttctcg atgacgacgc gcgaccagcc cggaccgcgc agtccatgtg
     2761 tgccgatcga ccggacgatc ggggcgtagg cggcgggcgg cgtcgcgagg taaaagagcc
     2821 ggttcccgtt ggtgccgagc tcggcgtcca cctccgcgag gcgcgccgcg agcgcgcggt
     2881 atccggcatc gtcgggtgtc gcgacgaagc gcatgttccg cgcgaacgac gaccagacct
     2941 cttcgttgat gggcttcgtg cgactgaact cggccaccgc cgcgtggagc tcggcgcgga
     3001 aggcctcgtc ggtcgagggg cggcgtgccg cgccgacgac cgcgaagctg gccgggagca
     3061 gccgctgcac cgcgaggttg tagagggccg gaaggagctt gcgcgcggcg aggtcgcccg
     3121 atccgccgaa cagcacgagc gtggccggct ccggcgtgcg cgcgaggcgc aggccctcgc
     3181 ggagcggatt gacgacggtg ggctgctcga cggcgctcat ccgcgtacgg cgatcttgct
     3241 cactcggtct tcaccgcgtg gccgccgaac tgattgcgca gggcggccgc gaccttcatc
     3301 gcgtacgagt cgtcctgccg cgaggtgaag cgcgcgaaga gcgagtgcgc gagcgcggtc
     3361 atcggcacac cgtgatcgat cgcctcgtgg atcgtccagc ggccctcgcc ggagtccgcg
     3421 acccagccct tgatgttcgc gagctcggtc ccctcctgct cgaaggcgcg caccgcgagc
     3481 tcgagcagcc aggagcgcac gacgctgccc tgcatccaca cgctcgcgac ccgcttcatg
     3541 tcgatctcga actcgctggc atgcagcagc tcgaagccct cgccgtaggc ctgcatcatc
     3601 ccgtactcga tcccgttgtg gaccatcttc acgaagtggc ccgcgccgct cttgccgaag
     3661 tgggaccagc cgtccggcgg cgcgagcgtg tcgagcgcgg gacggacgag tccgacggcc
     3721 cgctcggggc cgccgaccat catgcagtag cccacctcga ggccccacac gccgccggag
     3781 acgccgatgt ccaggaagtc gagaccttgc gcggcgtact tctcggcccg gcgcatcgag
     3841 tcacggaagt tggaattgcc tccgtcgatg agcacgtcgc ccttgctcgc gtgcctcgcg
     3901 aattcgtcga gcgcggcctc ggtggcgtcg cccgacggca ccatcgacca cagcacgcgc
     3961 ggcggcgtga gcgccctcac catctcctcg atcgtgtagg cggcgacggc gccctcgccc
     4021 ttcttctcgt cgaccgggcc ggggctccgg ttgcccgcga cgacacggtg gccgccgcgg
     4081 agcagccgcg tgaccatgtt cccgcccatg cggccgaggc cgtaaagacc gatgtccatc
     4141 gcgctcaccc ctctcgtgag ccgaggatat gacgttgcgg cgcgcgcctt gcggtaccgt
     4201 tagaacgcat ggcgacggcc accgcgaccc ccacgcgcct cgatgtgtac cgtcagctcg
     4261 cccgacagct tcgcgccgac agcatccgct gctcgacccc cgtcggatcc ggacatccga
     4321 cgtcgagcct gtccgcggcc gacctcatgg cggtcctgct cgcgggccat ctgcgctacg
     4381 actggaagaa gcccgacgat cccgcgaacg accacctgat cttctcgaag ggccacgcgt
     4441 ccccgctcgt ctacgcgctg ttccgcgcgg ccggcgcgat cgacgacgcg gagctcatga
     4501 cgttccggag gctcggttcg cggctgcagg gccatcccac gccgctcctg ccgtgggtcg
     4561 acgtcgcgac gggctcgctc ggccagggca tgccgatcgc ggtcgggatg gccctcgccg
     4621 cgaagcggct ctggaaggcg ccgttccaca tctggacgct cgtcggcgac agcgagagcg
     4681 ccgaaggctc ggtgtgggag ggtttcgacc tcggcgggca cgagagcctg gagaacgtca
     4741 cggcgatcct cgacgtcaac cgtctcgggc agcgcggtcc caccgagctg gagtgggaca
     4801 cggacgcgta tgccgcgcgc atcgagtcgt tcggctggga cgcgctggtg atcgacggcc
     4861 acgacctcgc cgagatcgac gatgcgctgg cgcgtgcgcg cgacgcgagc cggccgacgg
     4921 cgatcgtggc gcggacgaag aaaggccgcg gctactcgaa gatcgaggac cagcccggct
     4981 ggcacgggaa ggcgagctcg cccgaggacg ccgaggaggc gctccgcgag ctcggtggtg
     5041 tccgcaacga cgtgatcgc
//
LOCUS       DASTZR010000429         5358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_44105, whole genome shotgun sequence.
ACCESSION   DASTZR010000429 DASTZR010000000
VERSION     DASTZR010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5358
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44105"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="VFV20_09530"
     CDS             <1..985
                     /locus_tag="VFV20_09530"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HEU5288633.1"
                     /translation="EVPFQISRAVRTEKRVVVVELDEGGRISRGEGSPDPFFGETSES
                     IEADVRTSVGLIPEDPGDLATLKRRLDERFPHGGAAACALDVLGHDRAAQAAGVPLRT
                     FLGVGAGDAPPTSFTIGIAEPAVMADRAAAAAAKGFSVLKVKLGHGDDDTVLAGIRER
                     FRGTIRVDPNAAWTPDEAPERIRRIVRYGIEFVEQPTPPADIDGLRRVRERSELPIVA
                     DEAAVRVHDVERLATAGDGINVKLQKSGGVAEARAMIARAHELGLRVMLGCRAAETSV
                     AIAAAAHLAPSVEWADLDGNLLIVDDPFRAVPVVSGRFVFSDRPGLGVVPA"
     gene            982..1695
                     /locus_tag="VFV20_09535"
     CDS             982..1695
                     /locus_tag="VFV20_09535"
                     /inference="COORDINATES: protein motif:HMM:NF018964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1461 domain-containing protein"
                     /protein_id="HEU5288634.1"
                     /translation="MTAAVAPVPRAPLRLWPLELLLFALALAFSSIVTFALVWSGDAP
                     YRDLARDNHVARYTFVVTAPAGGRVETDLDGAVAIHQRWSQYVTGGIDEVPSFDPPLF
                     TDDEYSHMADVRRVFDLTKFFVPLALFVMIVRLQRARGRSPRDMWRLVRDGALVAVAI
                     VGLIALGAVFAFDQLFLLFHEVFFPQGNFLFDPATSNLLRVWPEWYWEGMFLRVGLSF
                     VALALVVAAVAALRLRAAK"
     gene            1712..2815
                     /locus_tag="VFV20_09540"
     CDS             1712..2815
                     /locus_tag="VFV20_09540"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEU5288635.1"
                     /translation="MKRVLLIEDLPQVAQHLQQMLQREQEIEVAGVQTQAEAAIAQAT
                     TEKPDIVMVDALLQGKVTGFDIAKRIREASPGTRIVMVTVPQRPVEPKPELGIDAVFV
                     LPGGANELGTALNVGKAAKVQAGTIVAVYSPKGGTGKTTLALNLACVLRRQGKAVALL
                     DGVMQFGAVRHVLQVPPTTRSFVDLPAAGAMKTSIGEVLWEGPGGVHILLAPARPEEA
                     DLVQATDIAAAMGLLAESHDYVIVDAPSRLSEDTLAILDAASLILIVVTYMGATVANA
                     RQAIDTFEALGYKGQKPILLVVNQSDTMAGMSKGGLEHALNLPVIAEIPTDWKTVSES
                     LNKQQPFVLSSPTAPVSKAVENLANALVSQQRK"
     gene            2817..3692
                     /gene="murI"
                     /locus_tag="VFV20_09545"
     CDS             2817..3692
                     /gene="murI"
                     /locus_tag="VFV20_09545"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEU5288636.1"
                     /translation="MGRAPTSEAAIAEQRADKRGTRSNTPIGVFDSGVGGLTVLKELR
                     RQLPSEATVYLGDEARMPYGPRPAGEVLAFTRQAMRWFAERDCKLIVIACNTSTSVAL
                     EAVRDEMRVPVIGVVRPGAAAAIAASTRRAIGVLATAGTVRSGAYVRAVRDLDPLCDV
                     IQQACPKLVPIVEAGKAGTPEALAAVREYVEPLLTEGAVVTPAVDTLLLGCTHYPLLR
                     EEFSKVAGPGVRVVDSATTTALAVREVLASHRLLSGPGRPAHEVYATGPRDRFRGIAR
                     TIFGEDVDVREATLG"
     gene            3689..4801
                     /gene="ychF"
                     /locus_tag="VFV20_09550"
     CDS             3689..4801
                     /gene="ychF"
                     /locus_tag="VFV20_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEU5288637.1"
                     /translation="MNLVTTITGLPYVGKTTLFNLLTGGHASTGAFAGAEAETNVGVA
                     KVPDERVDKLAALFRPKKTTYAEITYRDLGLTHSADKGDKQGLSPQKLGDLRSSDAIV
                     HVVRAFRDPSVPHVDTTVDPVRDLASVEIELLFADHGVVERRLERIEPELRSAKGAER
                     EAREREKAVLQKAMAALDAETPLRDVELDEEERKAIRGYRFLTLQPQLVVANVDESDV
                     ARPDAVLAPLRAAIAKHRAMAVVPVCAKIESEIAELPADEAAAFRADLGVSEPALQRV
                     IRATYELLGLISFFTTGEDEVRAWTIPAHTPAQEAAGAIHSDLERGFIRAEVIRWDEL
                     LKAGSEANAKKAGIMRTEGKSYRVSDGDCLHVLFNV"
     gene            4801..>5358
                     /locus_tag="VFV20_09555"
     CDS             4801..>5358
                     /locus_tag="VFV20_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288638.1"
                     /translation="MPVRFLAIGHVTRDEFPGETAWRLGGTATYTAATAARLGAHATL
                     VTRVGPNERATLEERCATLGIDLVALPSEVTTTFAFRYEDGRRILRLRRRARGLRLAD
                     VPETLRAPDAVILGSVAHEIDRSLLGAFGHAATVVTAQGYLREWDADGAIHSRRWSDV
                     AEVTASASAIVLSEEDVAGDLSEPRR"
BASE COUNT          758 a         1887 c         1948 g          765 t
ORIGIN      
        1 cgaggtcccc ttccagatat cgcgcgcggt gcgcaccgag aagcgcgtgg tcgtcgtcga
       61 gctcgacgag ggcggccgca tctcgcgtgg cgaggggtcg ccggacccgt tcttcggcga
      121 gacgagcgag agcatcgagg ccgacgtccg cacgtcggtc ggcctcatcc ccgaggatcc
      181 gggcgacctc gcgacgctga agcggaggct cgacgagcgc ttcccgcacg gcggcgcggc
      241 ggcgtgcgcg ctcgacgtcc tcgggcacga ccgcgccgcg caggcggccg gcgtcccgct
      301 gcggacgttc ctcggggtcg gcgccggcga cgcaccgccc acgtcgttca ccatcggcat
      361 cgccgagccg gcggtgatgg cggaccgcgc cgcggccgcg gccgccaagg gcttcagcgt
      421 gctgaaggtg aagctcgggc atggcgacga cgacaccgtc ctcgcgggga tacgggagcg
      481 gttccgcggg acgatccgcg tcgacccgaa cgcggcgtgg acgccggacg aggcgccgga
      541 gcgcatccgg cggatcgtcc gctacggcat cgagttcgtg gagcagccga cgccgccggc
      601 ggacatcgac ggcctgcgcc gggtccgaga gcggagcgag ctgccgatcg tcgctgacga
      661 ggcggccgtg cgcgtccatg acgtcgagcg cctcgcgacc gcgggcgacg ggatcaacgt
      721 gaagctgcag aagagcggcg gcgtggccga ggcgcgcgcg atgatcgcgc gcgcgcacga
      781 gctcggcctg cgcgtgatgc tcggctgccg cgcggcggag acgagcgtcg cgatcgcggc
      841 cgcggcgcat ctcgcgccgt cggtggagtg ggcggacctc gacggcaacc tgctcatcgt
      901 ggacgacccg ttccgtgccg tgcccgtcgt gagcgggcgc ttcgtgttct ccgatcggcc
      961 cgggctcggc gtcgtgcccg cgtgaccgcc gcggtcgcgc ccgtgccgcg cgcgccgctc
     1021 aggctgtggc cgctcgagct gctgctgttc gcgctcgcgc tggcgttctc gtccatcgtg
     1081 acgttcgccc tcgtgtggtc cggcgacgcg ccgtaccgcg acctcgcgcg tgacaaccac
     1141 gtcgctcgct acacgttcgt ggtcaccgcg ccggccggcg gtcgagtcga gacggatctc
     1201 gacggcgccg tcgccatcca tcagcgctgg tcgcagtacg tgaccggcgg catcgacgag
     1261 gtcccgagct tcgacccgcc gctcttcacg gatgacgagt attcgcacat ggccgacgtg
     1321 cgccgcgtgt tcgacctcac gaagttcttc gtgccgctcg cgctcttcgt gatgatcgtg
     1381 cgcctgcaac gcgcccgcgg caggagcccg cgtgacatgt ggcgtctcgt gcgcgacggt
     1441 gcgctcgtgg cggtggccat cgtcggcctc atcgcgctcg gtgcggtctt tgccttcgat
     1501 cagctcttcc tgctgttcca tgaggtcttc ttcccgcagg ggaacttcct cttcgatccg
     1561 gccaccagca acctgctgcg cgtctggccg gagtggtact gggagggaat gttcctgcgg
     1621 gtgggacttt cgttcgtcgc gctggcgctg gtcgtggcgg cggtggccgc gctgcggctc
     1681 cgcgccgcga agtgacctag actcgcccgc gatgaaacgc gtcctcctca tcgaggatct
     1741 tccgcaggtc gcccagcacc tgcagcagat gctccagcgc gaacaggaga tcgaggtcgc
     1801 cggcgtgcag acgcaggccg aggcggccat cgcgcaggcc acgacggaga agccggacat
     1861 cgtcatggtc gatgcgctgc tgcagggcaa ggtcaccggc ttcgacatcg cgaagcgcat
     1921 tcgcgaggcc tcgcccggca cacggatcgt gatggtcacg gtgccgcagc gcccggtgga
     1981 accaaaaccc gagctgggga tcgacgccgt cttcgtcctc cccggcggcg cgaatgagct
     2041 cggcacggcg ctcaacgtcg gcaaggcggc gaaggtgcag gccgggacca tcgtcgcggt
     2101 gtattcgccg aagggcggca cgggcaagac gacgctcgcg ctgaatctcg cgtgcgtgct
     2161 gcgccgccag ggcaaggcgg tcgccctgct cgacggcgtc atgcagttcg gcgcggtccg
     2221 tcacgtgctc caggtgccgc cgaccacgcg ctccttcgtc gaccttcccg cggcgggcgc
     2281 gatgaagacg agcatcggcg aggtgctgtg ggagggcccg ggcggcgtcc acatcctgct
     2341 cgcccccgcg cggccggagg aggccgacct cgtgcaggcc acggacatcg cggccgcgat
     2401 gggcctgctc gccgagagcc acgactacgt gatcgtggac gcaccgagcc ggctctcgga
     2461 ggacacgctt gcgatcctcg acgccgcgtc gctcatcctc atcgtcgtca cgtacatggg
     2521 cgcgacggtc gccaacgcgc gccaggcgat cgacacgttc gaggcccttg gctacaaggg
     2581 ccagaagccc atcctgctcg tggtgaacca gtccgacacg atggcgggca tgtccaaggg
     2641 cgggctcgag catgccctga acctcccggt gatcgccgag atccccaccg actggaagac
     2701 ggtcagcgag tcgctgaaca agcagcagcc gttcgtcctg tcgagtccga cggcgccggt
     2761 gagcaaggcg gtcgagaacc tcgcgaacgc cctcgtctcc cagcagcgca agtgatgtgg
     2821 ggagggcccc cacgagcgaa gcggcgatag cggagcagcg cgcggacaag aggggcacac
     2881 ggtccaacac gccgatcggc gtcttcgact ccggcgtcgg cgggctgacg gtcctcaagg
     2941 agcttcggag gcagctgccg tccgaagcga ccgtgtacct cggtgacgag gcgcggatgc
     3001 cgtacggccc acggcccgcg ggcgaggtgc tcgcgttcac ccgccaggcg atgcgctggt
     3061 tcgccgagcg cgactgcaag ctcatcgtca tcgcgtgcaa caccagcacg agcgtcgcgc
     3121 tcgaggccgt gcgcgacgag atgcgcgtgc cggtcatcgg cgtcgtccgg ccgggtgcgg
     3181 ctgccgcgat cgccgcatcg acgcgccgcg cgatcggcgt gctcgcgacc gcgggcaccg
     3241 tgcggtcggg cgcgtacgtg cgcgcggtcc gtgatctgga tccgctctgc gacgtgatcc
     3301 agcaggcctg cccgaagctc gtgccgatcg tcgaggcggg gaaggccggg acgccggagg
     3361 cactcgcggc cgtccgcgag tacgtcgaac ccctcctcac cgagggtgcg gtggtcacgc
     3421 ccgccgtgga cacgctgctc ctcggctgca cgcactatcc gctgctgcgc gaggagttct
     3481 cgaaggtcgc gggcccgggc gtgcgtgtcg tggactcggc gacgacgacc gcgctcgccg
     3541 tgcgggaggt cctggcgtcg catcggctgc tctcgggacc cggcaggccg gcccatgagg
     3601 tgtacgcgac gggcccgcgc gaccgcttcc gcggcatcgc ccgcaccatc ttcggcgagg
     3661 atgtggacgt gcgcgaggcg acgctcggat gaacctcgtc accaccatca cgggccttcc
     3721 gtacgtgggg aagaccacgc tgttcaacct cctcacaggc ggtcacgcgt cgaccggcgc
     3781 gttcgctggg gccgaggccg agacgaacgt cggcgtcgcg aaggtgcccg acgagcgtgt
     3841 cgacaagctc gccgcgctct ttcggccgaa gaagacgacg tacgcggaga tcacgtatcg
     3901 cgacctcggc ctgacgcact cggccgacaa gggcgacaaa cagggcctca gtccccagaa
     3961 gctcggcgac ctgcgcagct ccgacgccat cgtgcacgtc gtgcgcgcct tccgcgatcc
     4021 ctcggtcccg cacgtcgata ccacggtgga cccggtgcgc gatctcgcga gcgtggagat
     4081 cgagctcctc ttcgcggacc acggcgtcgt cgagcgccgg ctcgagcgca tcgagcccga
     4141 gctgcgctcg gcgaagggcg ccgagcggga ggcgcgcgag cgcgagaagg ccgtcctgca
     4201 gaaggcgatg gccgcgctcg acgccgagac cccgctccgc gacgtcgagc tggatgaaga
     4261 ggagcggaag gcgatccgcg gctaccgctt cctcaccctg cagccgcagc tcgtcgtcgc
     4321 caacgtcgac gagtccgacg tggcaaggcc ggacgcggtg ctcgcgccgc tgcgcgcggc
     4381 gatcgcgaag catcgggcga tggccgtcgt gccggtgtgc gcgaagatcg agtccgagat
     4441 cgccgagctg cccgcggacg aggccgccgc gttccgcgcg gacctcggcg tgagcgagcc
     4501 ggcgctgcag cgcgtgatcc gcgcgacgta cgagctgctc ggtctcatct cgttcttcac
     4561 caccggtgag gacgaggtgc gcgcgtggac catccccgct cacacacccg cgcaggaggc
     4621 ggccggcgcg atccactccg acctcgagcg gggattcatc cgcgcggagg tcatccgctg
     4681 ggacgagctg ctgaaagcgg gctcggaggc gaacgcgaag aaggccggga tcatgcgcac
     4741 ggaggggaag tcctaccgcg tcagcgatgg cgactgcctc cacgtgctct tcaacgtctg
     4801 atgccggtcc gcttcctcgc gatcggccac gtcacgcggg acgagttccc gggcgagacc
     4861 gcctggcgcc tcggcggcac ggccacgtac accgcggcca ccgcggcgcg gctcggcgcg
     4921 cacgcgacgc tcgtcacgcg cgtcgggccg aacgagcgcg cgaccctcga ggagcgctgc
     4981 gcgacgctgg gcatcgacct cgtcgccctt ccgtcggagg tgacgacgac gttcgcgttc
     5041 cgctacgagg acgggcggcg catcctgcgg ctgcggcgcc gtgcgcgcgg attgcgcctg
     5101 gccgacgtgc ccgagacgct gcgtgcgccg gatgcggtca tcctcggatc ggtcgcgcac
     5161 gagatcgatc gctcgctgct cggggccttc gggcacgcgg ccacggtcgt gaccgcgcag
     5221 ggctacctgc gcgaatggga cgccgacggc gcgatccact cgcggcgctg gagcgacgtc
     5281 gccgaggtga ccgcgagcgc gtcggcgatc gtgctgagcg aggaggacgt cgccggcgac
     5341 ctgtcggagc cccggcgc
//
LOCUS       DASTZR010000430         2145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_409837, whole genome shotgun sequence.
ACCESSION   DASTZR010000430 DASTZR010000000
VERSION     DASTZR010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409837"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="VFV20_09560"
     CDS             complement(<1..691)
                     /locus_tag="VFV20_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEU5288639.1"
                     /translation="MCGVFGIRGHPDAASLTHLGLYSLQHRGQESAGIVAVGDDGTAR
                     NLRAMGLVSDKFSPESIATLTGSTAIGHTRYSTTGSSTIENAQPVLARFRDGHIALAH
                     NGNLTNSSELRFELEDQGSIFASTMDSEVIVHRMARSRSAAPEEKLADALRGVEGAYS
                     AVVLAGDTLLAARDPRGWRPLVMGRLNGAVIFASETCALDIVGATFEREIEPGEIVAV
                     DGTGIRASRPLP"
     gene            complement(697..>2145)
                     /locus_tag="VFV20_09565"
     CDS             complement(697..>2145)
                     /locus_tag="VFV20_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEU5288640.1"
                     /translation="VTIDVSKVPVRESGMTPYEILLSESQERMLVVAKRGHENAVAEI
                     LQRWDLTAAVIGEVIAEPVYRVTEGDTVVAEFPGSRLVTDCPRYSPEARESEAIVGLR
                     ETDVASIPELAEEKDPLWTLEQMLSSPTIASKAWAYRQYDSTVRTNTVIGPGGDAAVL
                     RLRGTNRAIAVKTDCNGRYVYLSPRNGARAAVCEAARNVACTGAKPLAITNCLNFGNP
                     KRPENFFQLSEAIAGMGEACRALSTPVTGGNVSLYNESPSGAVYPTPVIGMLGLIDDI
                     AHVTPATWSVDDDVIVLLGELLGEPADGLGGSEYLARIHGTVAGTPPKCDPELERDTI
                     ACLLECIRAGHVRSAHDCSDGGLGVALAECAIANRSALVGAEIDVSAWGDVPLRELLY
                     NEAQARIIITVPESSLAEIARIAVKHMVPPIMIGRVRTGSPNLEIEHGEKRTLVAPLE
                     RLAAAYHDAIPAIMEGSVESAAVLEQAAVTTV"
BASE COUNT          296 a          770 c          729 g          350 t
ORIGIN      
        1 cggggagggg gcgggaggcg cggattccgg ttccatccac ggccacgatc tcgcccggct
       61 cgatctcccg ctcgaaggtc gcgccgacga tgtccagcgc gcaggtctcc gacgcgaaga
      121 tcacggcgcc gttgagccgc cccatcacca gcgggcgcca gccacgggga tcgcgggcgg
      181 cgagcagcgt gtcgccggcc agcacgaccg ctgagtacgc gccttcgacc ccgcgcagcg
      241 cgtcggccag cttctcctcc ggcgcggccg agcgcgaacg ggccatccgg tgcacgatca
      301 cttcggagtc catggtggac gcgaagatgg atccctgatc ctccagctcg aaccgcagct
      361 cgctggagtt ggtgaggttg ccgttgtgcg cgagcgcgat gtggccgtcg cggaaccggg
      421 cgagcaccgg ctgcgcgttc tcgatcgtgg acgacccggt ggtgctgtag cgtgtgtgcc
      481 cgatcgcggt cgagccggtg agcgtggcga tgctctccgg actgaacttg tcggacacga
      541 gccccatcgc gcgcaggttg cgcgcggtcc cgtcgtcgcc gacggcgacg attccggcgg
      601 actcctgccc gcggtgctgc agtgagtaca gcccgaggtg ggtgagcgag gccgcgtcgg
      661 gatggccgcg gatgccgaag acgccgcaca tgggcgctag accgtcgtca cggctgcctg
      721 ctccagcacg gcggcgctct cgacgctccc ttccatgatc gccgggatcg cgtcgtggta
      781 tgcggccgcc agtcgctcga gtggggccac cagtgtgcgc ttctccccgt gctcgatctc
      841 gaggttgggc gagcccgtac gcacgcgccc gatcatgatc ggcggcacca tgtgcttgac
      901 cgcgatcctc gcgatttccg caagcgaaga ctcaggcacg gtgatgatga tcctcgcttg
      961 tgcttcgttg tacagcagct cccgcagcgg cacgtcgccc cacgccgaga cgtcgatctc
     1021 cgcgccgacc agcgccgacc ggttggcgat cgcgcactcc gcgagcgcga cgccgagccc
     1081 gccgtcgctg cagtcgtgcg ccgagcggac gtgcccggcg cggatgcact cgagcaggca
     1141 cgcgatcgtg tcgcgctcca gctccggatc gcacttcggc ggagtgcccg cgaccgtgcc
     1201 gtggatccgg gcgaggtact cgctgcctcc gagaccgtcg gccggctcgc cgaggagctc
     1261 gcccagcagg acgatcacgt cgtcgtccac ggaccaggtc gccggcgtca cgtgggcgat
     1321 gtcgtcgatc agcccgagca tcccgatgac cggggtcggg tacaccgcgc cggacgggct
     1381 ctcgttgtac agcgagacgt tgccgccggt caccggcgtg ctcagagcgc gacacgcttc
     1441 gcccatcccc gcgatggcct cgctgagctg gaagaagttc tccggccgct tcgggttgcc
     1501 gaagttgagg cagttggtga tcgcgagcgg cttggcgccg gtgcacgcga cgttcctcgc
     1561 cgcctcgcac acggccgccc gcgcgccatt cctcggcgac agatagacgt accgcccgtt
     1621 gcagtcggtc ttcaccgcga tcgcgcgatt cgtcccgcgc agccgcagta ccgccgcgtc
     1681 gccgcccggc ccgataaccg tgttggtgcg tacggtcgag tcgtactgcc ggtacgccca
     1741 cgccttgctg gcgatcgtcg gcgacgagag catctgctcg agcgtccaga gcgggtcctt
     1801 ctcttctgcc agctccggga tgctcgcgac gtcggtttcg cgcagcccaa cgatcgcctc
     1861 gctctcgcgc gcttccggcg agtagcgcgg gcagtcggtg acgagccgtg agccggggaa
     1921 ctcggccacc accgtgtcgc cctccgtcac ccgatacacg ggctccgcga tgacctcgcc
     1981 gatcacggcc gcggtgaggt cccaccgctg gaggatctcc gccaccgcgt tctcgtgccc
     2041 gcgcttggcg acgaccagca tgcgctcctg cgattcgctc aacagaatct cgtacggagt
     2101 catccccgac tcgcgcaccg gcactttgct cacgtcgatg gtgac
//
LOCUS       DASTZR010000431         6203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_854422, whole genome shotgun sequence.
ACCESSION   DASTZR010000431 DASTZR010000000
VERSION     DASTZR010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6203
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854422"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1320
                     /locus_tag="VFV20_09570"
     CDS             <1..1320
                     /locus_tag="VFV20_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288641.1"
                     /translation="VAGLIAAFQPIADTDMWWHLATGRETLAHGFVRADVFSWSVRGA
                     PVSTDQWLGQVVMYATWLATGWRGIAVLRIVLVTLLVAFVALGAARTATRPLAVTLAT
                     VPALLLTRAVWVDRPELAGFTFFAALLFILRVGREDGGRTYVLCALLLGIWANVHGSF
                     ALGVAITVLVCLERAWRDVPRRRVHVAAAIAAVAVTFANPAGAGVWTAPGLHLLSPPR
                     DIQEWNLIDVATPLGIAYAATLTLVVLSAFAGPRLPLRDLIVLVPVAFLSLTAVRQAP
                     LLAVAAAPLFADRAERLLTRAGLSAPRRGSGAASQLAFVPALILTLVALLVAPAQPDE
                     RAYPVAAVGALPSGDGTLARYEWGGWLIWHEVPVFVDGRLTPYLGAVLDDYRRIIAAG
                     PGWEDALRRQHVRTLLVVGSDPVASRARELGWSVRAFEGGAVVIVVP"
     gene            1364..2416
                     /locus_tag="VFV20_09575"
     CDS             1364..2416
                     /locus_tag="VFV20_09575"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU5288642.1"
                     /translation="MIDDAPCAQSASYTRLTSTQPTRLIKDGDDLEAQFDAIVDALRR
                     DVRRTRQQVDKFARAYQRTAEAQSRRSADGKPDEPTQKAYRRLKQLQLVVQHIESSAA
                     YLLGSGIAPEVTGDHDQTLEVAQRVLESLEAERERLYRDVHDGPAQVLANAIFEVEYL
                     ERIADRAPSEVRQTLKAELGGLKTQFRSSLDSVRAMIYDLRPPELSELGLADAMRNYA
                     SEWEARCGLKVLATLDTTPTDLSPQHELAVYRVLQEALQNVHKHAHATTVGVVWQRTS
                     AAWTLHVTDDGLGFDLVKAARRAKSVGLLSMRERAELIGGSLQIQSMPGKGTAVTLLL
                     PVGHAERGSLLGRQQE"
     gene            2397..3104
                     /locus_tag="VFV20_09580"
     CDS             2397..3104
                     /locus_tag="VFV20_09580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5288643.1"
                     /translation="MADNKSDRAIRVLLADASRLTAMGIGQVLEREPDVDLVATASDG
                     EEAVTKTLELEPDVLVIEADLPRLSGIKVTQRVRRELPHVGVVVLTAKDEEQTLFEAI
                     RAGAAAYLNKDCDPVELVDAIRKARAGQFIINEKVFSRPAVASKVLAEFRELSVYGPG
                     SSHVFAPLSPREVQILDNIAQGMTNKEVAYALAISEQTVKNHMSSILRKLSVNDRTQA
                     VVYAIRQGWIKGPEMGN"
     gene            3107..4132
                     /locus_tag="VFV20_09585"
     CDS             3107..4132
                     /locus_tag="VFV20_09585"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU5288644.1"
                     /translation="MATDTAADLATEQQRIAAEIRELDLLVESTQTEVNRLKAREEQT
                     KLRVSEVRGNPGNFQREEIFGATDDYATAIGRRMTMEGQLGTLQAKRKLLDRAKSLVS
                     AAQQHVRDNAAAPLPADASPVIDESRLLQAVVDTQEAERKRIARQVHDGPAQAMANVV
                     LQSEISERLFEVDPQRSRTELAALRQMVNKTLQELRGFIFELRPMILDDLGLVPTMRR
                     YVQTLVDKHGIRIDFSSTGRDRRLPSDDEVAIFRLIQDSLVERIEKGASKDIVLGMGW
                     KDEQLDIMLQADGKELPPDGELQSGLRRSERLELLHGESTHETRADGTVVLSVKVPIR
                     AAPAIIT"
     gene            4129..4641
                     /locus_tag="VFV20_09590"
     CDS             4129..4641
                     /locus_tag="VFV20_09590"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5288645.1"
                     /translation="MSPAFTAEQIAALLESTCALIDAEMRALGDDGCRFHHKAGEWCV
                     NECVGHLIEAEKRGFAGRIRDLLAGRPIESWDQAQVARDRRDCERVSQSLWMEFMGLR
                     NSSIALVRSLTAADMDRGGTHPKVGMLRVRDLLQEWVHHDRNHTKQLLSIQQERAYPH
                     MGNAQKFVGE"
     gene            complement(4724..>6203)
                     /locus_tag="VFV20_09595"
     CDS             complement(4724..>6203)
                     /locus_tag="VFV20_09595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEU5288646.1"
                     /translation="LAAVLVVTCVLSLAPRVAAAASLHAVPATVRVNVTPNGVGYTVV
                     SSSGTLTATRADGALVYRGRERLLVRTNVRRAEGVPLSLPPRPDALTPDERADRRRLL
                     HEARSAELEARSQPASIVTTPFEISLLRDLDDPQGEPLHSADKVGVVTFTTDGILAVN
                     GRGFRGVLETTVDDDGEAIIVNTVATGVYLASVVGSEEPSSWEPQALAAQAIAARTYL
                     LTHLRRHDHYDLEGDTRDQEYDGVANEVRSTVRAVELTAGIVATYRGAAIEALYSANA
                     GGITEDSENVFTNALPYLRSVPSPADEVAKSSAFGRSSWEWEREFTAPMLGDYMRARG
                     LDVGTPTKIEVLRKSATGRPLLTRVTGTLGSRDIGMERARYYFGLKSNLFTPTQQPSE
                     QQWVDAGDIERLRDMAALGARKLRSNRDVSFDDTGRRRGMVVTEILFALPARFSFVGR
                     GFGHSVGMSQWGAQGMALGGASYEQILKHYYTGIALTNVGGP"
BASE COUNT          927 a         2181 c         2167 g          928 t
ORIGIN      
        1 gtcgcgggtc tgatcgccgc cttccagccg atcgccgaca cggacatgtg gtggcatctc
       61 gccacggggc gcgagacgct cgcgcacggg ttcgtgcgcg ccgacgtgtt ctcgtggtcg
      121 gtccgcggtg cccccgtctc caccgaccag tggctcggcc aggtcgtgat gtacgccacg
      181 tggctcgcca ccggctggcg cggcatagcg gtgctccgca tcgtgctggt cactctgctc
      241 gtcgcgttcg tcgcgctcgg cgcggcgcgg accgcgacgc ggccgctcgc cgtgacgctc
      301 gcgacggtac cggcgctgct gctcacgcgg gcggtgtggg tcgatcggcc cgagctcgcc
      361 ggcttcacgt tcttcgccgc gctgctgttc atcctccgcg tcggccgtga ggacggcggt
      421 cggacgtacg tcctctgcgc gttgctcctg gggatctggg cgaacgtcca cggctcgttc
      481 gccctcggcg tggcgatcac cgtcctggtg tgtctcgaac gcgcgtggcg cgacgtccca
      541 cggaggcgcg tgcacgtggc tgccgcgatc gcggccgtcg ccgtcacgtt cgcgaacccc
      601 gcgggcgccg gcgtgtggac cgccccgggc ttacatctgc tctctccgcc gcgcgacata
      661 caggagtgga acctcatcga cgtcgccaca ccgctcggta tcgcgtacgc ggcgaccctc
      721 acgctcgtcg tgctgtccgc gttcgccggc ccgcgtcttc cgctccgcga cctgatcgtc
      781 ctggtgcccg tcgcgttcct gtcgctcacc gcggtgcggc aagcgccact gctcgcggtc
      841 gcggcggcgc cgctcttcgc cgaccgcgcc gaacgcctcc tgacgcgggc gggtctttcg
      901 gcgccccgtc ggggaagcgg cgccgcctca caactcgcat tcgtgccggc gctgatcctc
      961 acgctcgtcg cgctgctcgt ggcgccggcg cagccggacg agcgcgccta cccggtcgcg
     1021 gccgtcggcg cgcttccgtc cggtgacggg acgctcgccc gctatgagtg gggcggctgg
     1081 ctgatctggc acgaggttcc cgtcttcgtg gacgggcgcc tcacgccgta cctcggtgcg
     1141 gtgctcgacg actatcgacg catcatcgcg gctgggccgg gttgggaaga tgccctgcgc
     1201 cgtcagcacg tgcgtacgct tctcgtcgtc gggtcggacc ccgtcgcgag ccgcgccaga
     1261 gagctcggct ggagcgtccg ggccttcgag ggcggcgccg tcgtcatcgt cgtgccgtga
     1321 gccgcccctt cccacggcag ccggtcgcag atcggtggag agcatgatcg acgacgcgcc
     1381 gtgcgcgcag agcgcttcct atactcgcct cacgagcacg cagccgaccc ggctcatcaa
     1441 ggacggcgac gacctcgaag ctcagttcga cgcgatcgtc gacgcacttc ggcgtgacgt
     1501 ccgtcgcacc cgccagcagg tcgacaagtt cgcgcgggcg taccagcgaa cggccgaggc
     1561 gcaatcgagg cggtccgccg acggcaagcc cgacgagccg acccagaagg cctaccgcag
     1621 gctgaagcag ctgcagctcg tggtgcagca catcgagtcg agcgccgcgt acctccttgg
     1681 ctcgggtatc gcgccggagg tgaccggcga ccacgaccag accctcgagg tcgcgcagcg
     1741 cgtgctcgag agcctcgagg cggagcgcga gcgtctgtat cgcgacgtgc acgatggtcc
     1801 cgcccaggtc ctggcgaacg cgatcttcga ggtcgaatac ctcgagcgca tcgcggaccg
     1861 cgccccctcc gaggtccggc agacgctcaa ggcggagctc ggcggcctga agacgcagtt
     1921 ccggtcctcc ctcgactccg tccgcgcgat gatctacgac ctccgtccgc cggagctcag
     1981 cgagctcggg ctcgcggacg cgatgcgcaa ttacgcgagc gagtgggaag cccgctgcgg
     2041 cctcaaggtg ctcgcgacgc tcgacaccac accgacggac ctctcgccgc agcacgagct
     2101 cgccgtctac cgcgtcctgc aggaggcgct gcagaacgtg cacaagcacg cgcacgcgac
     2161 cacggtgggc gtcgtctggc agcgcacgag cgcggcctgg acgctccacg tgacggacga
     2221 cggcctcggg ttcgacctgg tgaaggccgc gcggcgcgcc aagtccgtcg gcctgctctc
     2281 catgcgcgag cgggccgagc tcatcggtgg ttcactgcag atccagtcga tgcccgggaa
     2341 gggcaccgcg gttacactcc tgctccccgt ggggcacgca gagagaggaa gtctgcttgg
     2401 ccgacaacaa gagtgatcgc gcgatccggg tcctgctagc cgacgcgagc cgcctcaccg
     2461 cgatgggcat cggacaggtg ctcgagcggg agccggacgt cgacttggtc gcgacggcat
     2521 ccgacggcga agaggccgtc acgaagacgc tcgagctcga gcccgacgtc ctcgtcatcg
     2581 aggccgacct cccgcgcctc tcgggcatca aggtgactca gcgcgtccgc cgcgagctcc
     2641 cgcacgtcgg cgtcgtggtg ctgacggcga aggatgagga gcagacgctc ttcgaggcca
     2701 tccgcgcggg cgcggcggcc tacctgaaca aggactgcga tccggtcgag ctcgtcgatg
     2761 cgatccgcaa ggcgcgggcc ggccaattca tcatcaacga gaaggtcttc agcaggccgg
     2821 ccgtggcgtc gaaggtgctc gccgagttcc gcgagctgtc ggtctacggg cccggctcgt
     2881 cgcacgtctt cgcgccgctg tcgccgcgtg aggtgcagat cctcgacaac atcgcgcagg
     2941 gcatgaccaa caaggaggtc gcctacgcgc tcgcgatctc cgagcagacc gtgaagaacc
     3001 acatgtcgag catcctgcgc aagctctcgg tgaacgaccg cacgcaggcc gtcgtctacg
     3061 cgatccggca gggttggatc aagggaccgg agatggggaa ttgaccgtgg cgaccgacac
     3121 ggccgccgat ctcgccaccg aacagcagcg gatcgccgcc gagatccgtg agctcgacct
     3181 gctcgtcgag tccacgcaga ccgaggtgaa ccgcctgaag gcgcgcgagg agcagaccaa
     3241 gctgcgggtc tcggaggtcc gtggcaatcc cggcaacttc cagcgtgaag agatcttcgg
     3301 cgccaccgac gactacgcga ccgcgatcgg ccggcgcatg acgatggagg gccagctcgg
     3361 gacgctgcag gcgaagcgaa agctgctcga tcgcgcgaag agcctcgtct cggccgcgca
     3421 gcagcacgtc cgcgacaacg cggcagcgcc cctaccggcg gacgcctcgc cggtcatcga
     3481 cgagagcagg ctgctgcagg ccgtcgtgga cacgcaggag gcagagcgca agcgcatcgc
     3541 gcgacaggtg cacgacggac cggcgcaggc catggccaac gtggtgctgc agtcggagat
     3601 cagcgagcgc ctcttcgagg tcgatccgca gcgctcccgc accgagctgg ccgcgctccg
     3661 ccagatggtg aacaagacat tgcaggagct gcgcggcttc atcttcgagc tgcggccgat
     3721 gatcctcgac gacctcggcc tcgtcccgac gatgcgccgt tacgtgcaga cgctcgtcga
     3781 caagcatggg atacggatcg acttctcgag cacgggccgc gaccgccggc tgccgtccga
     3841 cgacgaggtg gcgatcttcc gcctcatcca ggactcgctc gtcgagcgga tcgagaaggg
     3901 cgcgagcaag gacatcgtcc tcggcatggg gtggaaggac gaacagctcg acatcatgct
     3961 ccaggccgac ggcaaggagc tcccgccgga cggcgagctg cagtccggcc tgcgtcgcag
     4021 cgagcggctc gagctgctgc acggagagtc cacgcacgag acgcgcgccg acgggacggt
     4081 cgtcctctcg gtgaaggtcc cgatccgcgc cgcgcccgcc atcatcacgt gagcccggcg
     4141 ttcaccgccg agcagatcgc cgcgctcctc gagtccacgt gcgcgctgat cgacgcggag
     4201 atgcgcgccc tcggcgacga cggttgccgc ttccaccaca aggccggcga gtggtgtgtg
     4261 aacgagtgtg tcggccacct catcgaggcc gagaagcgcg gcttcgccgg tcgcatacgg
     4321 gatcttttgg ccgggcggcc gatcgagtcg tgggatcagg cccaggtcgc gcgggaccgc
     4381 cgggactgcg agcgcgtcag ccagagcctc tggatggagt tcatggggct tcgcaacagc
     4441 tcgatcgcgc tcgtccggtc gctgacggcc gccgacatgg accgcggcgg cacgcatccg
     4501 aaggtcggga tgctgcgcgt gcgcgacctg ttgcaggagt gggtccatca cgaccgcaac
     4561 cacaccaagc agctgctgag catccagcag gagcgcgcat atccgcacat ggggaacgct
     4621 cagaagttcg tcggagagtg acgacaccgt cgcggctcgc ttcttcgcgc tgcgcgtgga
     4681 tgaggagttg cgcccacgct cgcgcacgct gacccgccgg cggctacggc ccgccgacgt
     4741 tcgtgagcgc gatccccgtg tagtagtgct tcaggatctg ctcgtagctc gcaccgccga
     4801 gcgccatgcc ctgcgcaccc cactgcgaca tgccgacgct gtggccgaag ccgcgcccga
     4861 cgaacgagaa gcgcgcgggt aacgcgaaga ggatctccgt cacgaccatg ccacggcgcc
     4921 ggccggtgtc gtcgaagctg acgtcccggt tcgatcgcag cttccgcgct ccgagcgcgg
     4981 ccatgtcgcg cagccgttcg atgtcgcccg cgtcgaccca ctgctgctcg gacggctgct
     5041 gcgtgggggt gaacaggttg ctcttcaggc cgaagtagta gcgggcgcgc tccatgccga
     5101 tgtcgcgcga gccgagcgtg ccggtgacgc gcgtgagcag cggccgcccg gtcgcgctct
     5161 ttcggagcac ctcgatcttc gtcggcgtgc ccacatcgag gccgcgcgcg cgcatgtagt
     5221 ccccgagcat cggcgcggtg aactcgcgtt cccattccca gctggaccga ccgaacgcgg
     5281 agctctttgc aacctcgtcc gcgggcgatg gcacgctccg aaggtagggg agcgcgttcg
     5341 tgaagacgtt ctcggaatcc tcggtgatgc cgcccgcgtt ggccgagtac agcgcttcga
     5401 tcgccgcacc gcggtacgtc gcgacgatgc cggcggtcag ctcgaccgcg cgcaccgtcg
     5461 agcgcacctc gttcgcgacg ccgtcgtatt cctgatcgcg cgtgtcgccc tcgaggtcgt
     5521 agtgatcgtg cctccgcagg tgggtgagga ggtacgtgcg ggcggcgatg gcctgcgcgg
     5581 cgagagcctg cggctcccag ctcgacggct cctcggagcc gaccaccgac gcgaggtaca
     5641 cgcccgtcgc gacggtgttc acgatgatcg cctcgccgtc gtcgtccacg gtggtctcga
     5701 ggacgccgcg gaacccgcga ccgttgaccg ccaggatccc gtccgtcgtg aacgtgacga
     5761 cgcccacctt gtcggcggag tggagcggct ctccctgcgg atcgtccagg tcgcgcagga
     5821 gcgagatctc gaagggcgtg gtgacgatcg aggccggctg cgagcgggcc tcgagctcgg
     5881 cgctccgggc ctcgtgcagc aggcgccgcc ggtccgcgcg ttcgtcgggc gtgagcgcgt
     5941 ccggtctcgg tggaagcgac aggggcacgc cctcggcgcg ccggacgttc gtgcggacga
     6001 gcagccgctc ccgtccgcga tacacgagcg cgccgtcggc ccgtgtcgcc gtcagggtcc
     6061 cgctcgagga cacgacggtg tagccgacgc cgttcggcgt gacgttgacg cgcaccgtcg
     6121 cggggacggc gtgcagcgat gcggccgccg cgacgcgcgg cgcgagggac agaacgcacg
     6181 tgacgacgag gacggcggcg agg
//
LOCUS       DASTZR010000432         6702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_546154, whole genome shotgun sequence.
ACCESSION   DASTZR010000432 DASTZR010000000
VERSION     DASTZR010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6702
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546154"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VFV20_09600"
     CDS             <1..224
                     /locus_tag="VFV20_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEU5288647.1"
                     /translation="AFRFLDALELAASATTLGDLFSEVLYPAISSHRRVAPEERARLG
                     IGDGTLRLSVGIEDPDDIVADLDAALGRV"
     gene            complement(221..1081)
                     /locus_tag="VFV20_09605"
     CDS             complement(221..1081)
                     /locus_tag="VFV20_09605"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEU5288648.1"
                     /translation="MTAPTMAMPAPQRTPSIAFPLVLISLGVAFLLVNMGYLARISWA
                     ELARLWPVLIILAGVDLLLRPRSFAAAAIAEIAIVVATFAYLFTATTLAPASLAYTVN
                     VPRAAATELSLTVNYGAGTLTLAGGGTDLVAVRSSEQDVKQTVDQTGSFAGVTLSSRE
                     DGTFWGRDRNWDVRLPSDVQTGMTLNLGAGDFDVDLTNVRTSRATINAGASDLTVTLP
                     KPSGNVPMTISGGASSLTLIVPAGVEYAVTTKGVMHSVQGVTQSAGYATAADRVTIEV
                     SIAAGSVTIR"
     gene            complement(1078..1464)
                     /locus_tag="VFV20_09610"
     CDS             complement(1078..1464)
                     /locus_tag="VFV20_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU5288649.1"
                     /translation="MENVSPTPAPRLYRSATDKVIGGVCGGLAAYFKLDPVFVRLAFV
                     LFALAGGASLLLYIVLWIAVPIGDTIPVATRGQSAETTAMILIGIGMVWLLANFGVFR
                     FINWSLAWPLVLIALGVALLLRRSRS"
     gene            complement(1657..3390)
                     /locus_tag="VFV20_09615"
     CDS             complement(1657..3390)
                     /locus_tag="VFV20_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288650.1"
                     /translation="MAAGDTRNWCRSDLLVDWLDLYGTGHGFTRDRDLGGYDERFDLA
                     RFLMQQGRGFERALIADLVARWEVLRVASRPDQARSFDAATATWEAMRAGVPIIAGAV
                     VRDPQLRMFGVIDLLVRSDVLAELCVDAFAGDPLELPVIGLSHGRHYRVVDVKFQTLE
                     ILKNGDLTTSAGELDTLAQLWVYNEALGRLQGYTPPAAYVVGRGWRQGEHRGDRCWER
                     LGRVRHDVYLRGRGMELRELVQQSAAWVRRVRTEGAEWRVLPLPSVPELWPNMKAEDA
                     GWHDAKVRIARELADLTILPRVGLGERARAHAVGITRWDDPRLSAALLGVTEREAPIV
                     DAVLAVNREGGPPVRPERLRDGNWRVHAPVEVYVDFEFLQDLADDFSAFPRKGGQALI
                     FQIGCGTYRDGAWRFRQFTVDDLSLDAEARMIDDWLAYIRGLCADRACELGEARLVHW
                     SPAEQTNFERAYDNARARHPDREWPPLPWYDLLRDVRSAPVVVRGAFSFSLKSIARAM
                     RAQGAIATDWGEGLADGAGAMAGAWNAAVEAKRRGTALGDTDTMREVGRYNEVDCRVM
                     AEILDHLRREH"
     gene            complement(3476..4084)
                     /locus_tag="VFV20_09620"
     CDS             complement(3476..4084)
                     /locus_tag="VFV20_09620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEU5288651.1"
                     /translation="MRYRAVGFDLLTALLDTWSLWATVAGDRALGMRWHAASQGLLRG
                     QPYRSYADITRESAAAVGIPRGTADEFLARFGEFAPWPDVPPVLERLGDRRRFVVTNC
                     SRELGERAARRAGTFDLVVTAEEAGAYKPDPRPYRLALERLALDPAEVLFVAGSAHDV
                     GGAVRVGMDVYWANRGNVPPPTDAKALRVEPDFRGLLDVLEG"
     gene            complement(4103..4909)
                     /locus_tag="VFV20_09625"
     CDS             complement(4103..4909)
                     /locus_tag="VFV20_09625"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU5288652.1"
                     /translation="MRNALVAFALVLASCAPAATPARDPLAGVYKIGGGDASIEIVTA
                     LTDAYAAKHPGVKFDIDTSLGSDPAVKLAADGSLDLGMASRELTNDESALVERHLIGV
                     AGTGFAVNAQNPIKYLSTAQLVEIYTGKTVDWSMLGVQNGTIMPMVREIGSSARTTFE
                     NVAFKGKPLYGPSVLEIKGGDQMRQAIASYRGAIGIIGVSSDDPKAEGVRLVGIDGVM
                     PTKATLMDGSYKLRRPLYLLVTRTGRPKPAISAFLEFVNGSEGQKIIAGF"
     gene            5007..6551
                     /locus_tag="VFV20_09630"
     CDS             5007..6551
                     /locus_tag="VFV20_09630"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEU5288653.1"
                     /translation="MSMVRSDWLHAKTEPVARNGMVTAEHHLAAEAGVEVMRDGGNAV
                     DAAVAAAFVMGVVEPFTSGIGGIAGLVVRRADGSVTSIEGGTRAPKAARPDMFQLVGG
                     GARAGMYAWPAVKDDANIEGPLSVGVPGQVAALCLAHERYGRLPRSRVMEPAVRIARE
                     GFEIDWYVSLSLAMYHERLHRSGDGGEIFFRRSGAPLRPPMGTEPSDRLVQPALATAL
                     ETIARDGPRVLYEGELGRAIVEGVRACGGVLSFDDLRGYAAAETAPLATMYRGHRVAT
                     LPGLTGGPTVARTLAILEAFPVGANEQGGAEHLHLVAEALRAAFLQRFSSLADHTTHI
                     NAVDGDRTLVSLTQTLGQGFGSGFVPKGTGIVLLDTMTWFDPVPGHPNSIGPEKRVLW
                     AGAPTIVLRGHAPLLAVGAPGGRKIMSAVAQSIVNVVDFGDGPLDAVNRPRVHDEGEG
                     LLVDTRVPEAVRAELADMGHAVQLKEETLMSAWFARPQAILVDPKTGDLRGGVDALKP
                     AAAIGY"
BASE COUNT          998 a         2353 c         2371 g          980 t
ORIGIN      
        1 gcgcgttccg cttcctcgac gcgctcgagc tcgcggcatc cgcgacgacg ctcggcgatc
       61 tcttcagcga ggtgctctac ccggcgatct cgtcgcaccg ccgcgtcgcg ccggaggagc
      121 gcgcgcgcct cgggatcggc gatgggacgc tgcggctctc cgtcggcatc gaggatccgg
      181 acgatatcgt cgcggacctc gatgccgcgc tcggtcgcgt ctagcggatt gtcaccgatc
      241 ccgccgcgat cgagacctcg atcgtcacgc ggtcggccgc ggtcgcgtag cccgcggact
      301 gcgtgacgcc ctgaacgctg tgcatgacac ccttcgtcgt cacggcgtac tccacgccgg
      361 cgggaacgat gagcgtcagc gaggacgcgc cgccggagat cgtcatcggg acgttgcccg
      421 acggcttcgg gagcgtgacg gtgaggtccg acgcacccgc gttgatggtc gcgcgcgacg
      481 tccgcacgtt ggtgagatcg acgtcgaagt cgccggcacc aaggttgagc gtcatgccgg
      541 tttggacgtc gctcggcagc ctcacgtccc aattgcggtc gcgaccccag aacgtgccgt
      601 cctcacgtga cgagagcgtc acaccggcga acgacccggt ctggtccacc gtctgtttca
      661 cgtcctgctc cgacgaccgg acggcgacga ggtcggtgcc gccaccggcc agcgtgagcg
      721 ttccggcgcc gtagttcacg gtgaggctga gctcggtcgc cgccgcgcgc ggaacgttca
      781 ccgtgtacgc gagcgacgcg ggcgcgagcg tcgtggccgt gaagagatac gcgaacgtgg
      841 cgacgacgat cgcgatctcg gcgatcgccg cggccgcgaa cgagcgtgga cgaaggagca
      901 gatcgacgcc cgccaggatg atgaggacgg gccagagcct cgcaagctcg gcccagctga
      961 tacgcgcgag atagcccatg ttcacgagca ggaaggcgac gccgagcgag atgagcacga
     1021 gcgggaaggc gatgctgggc gtgcgttgcg gcgcgggcat ggccatcgtc ggtgcggtca
     1081 tgatcgtgac ctccgcagga gcagcgcgac gccgagtgcg atcagcacca gcggccacgc
     1141 gaggctccag ttgatgaatc ggaagacgcc gaagttcgcg agcagccaca ccatgccgat
     1201 gccgatgagg atcatcgccg tcgtctccgc ggattgcccg cgtgtggcca ccgggatcgt
     1261 gtcaccgatc gggaccgcga tccacagcac gatgtacagc aggagcgacg cgccccctgc
     1321 gagcgcgaac agaacgaacg cgagccggac gaacaccggg tcgagcttga aataggccgc
     1381 gagcccgccg cagacgccgc cgatgacctt gtcggtcgcg cttcggtaca ggcgtggggc
     1441 gggtgttggt gagacgttct ccatgtctcc acctcctcta tgacacatac ggcgtaccgc
     1501 gtccgggcat tgcgcaggag cgtcgcggtg ccccaggcgc tgtcggggtc ggcaccacat
     1561 ccggaccgcc gaatggcccc gcacgtgacg cggcggtggc ctccgcgtcc gcctgtgtag
     1621 cgaaggacac ggtcgcgcgc cgtccgcccg tgtggtctag tgctcccggc gcaggtggtc
     1681 gaggatctcc gccatgacgc ggcagtcgac ctcgttgtag cggccgacct cccgcatcgt
     1741 gtcggtatcg ccgagcgcgg tgccgcgccg tttcgcctcg accgcggcgt tccacgcgcc
     1801 ggccatcgcg ccagccccgt cggcgaggcc ctcgccccaa tcggtcgcga tcgcgccctg
     1861 ggcgcgcatg gcgcgcgcga tcgacttgag cgagaacgag aacgcgccgc gcaccaccac
     1921 cggcgcggac cgcacgtctc gcagcaggtc gtaccacggc agcggcggcc actcgcgatc
     1981 ggggtggcgc gcgcgcgcgt tgtcgtacgc gcgctcgaag ttcgtctgct ccgccggtga
     2041 ccagtggacg agccgtgcct cgccgagctc gcacgccctg tccgcgcaga gcccgcggat
     2101 gtacgcgagc cagtcgtcga tcatgcgcgc ttccgcgtcg aggctgaggt cgtccacggt
     2161 gaactgccgg aagcgccagg cgccgtcccg ataggtcccg cagccgatct ggaagatgag
     2221 cgcctggccg cccttccgcg ggaacgcgct gaagtcgtcc gcgagatcct gcaggaactc
     2281 gaagtccacg tacacctcga ccggcgcgtg cacgcgccag ttcccatcac gcaggcgctc
     2341 gggacgcacc ggcggtccac cctcacggtt gaccgccagc accgcgtcca cgatcggcgc
     2401 ctcgcgctcc gtgacaccga gcagcgcggc cgagaggcgc gggtcgtccc agcgggtgat
     2461 gccgacggcg tgcgcgcggg cacgctcacc gagcccgacc cgcggcagga tggtcagatc
     2521 cgcgagctct cgcgcgatcc gcaccttcgc gtcgtgccag ccggcgtcct cggccttcat
     2581 gttcggccag agctccggga ccgacgggag ggggagcacg cgccactccg cgccctcggt
     2641 gcgcacgcgc cggacccatg ccgccgactg ctgcaccagc tcgcggagct ccatgccgcg
     2701 gccacgcagg tacacgtcgt ggcgcacccg tccgaggcgc tcccagcagc ggtccccgcg
     2761 atgctcgccc tgccgccatc cacggccgac gacgtacgcc gcgggcggcg tgtatccctg
     2821 gaggcgcccg agcgcctcgt tgtagaccca gagctgcgcg agcgtgtcga gctcgcccgc
     2881 cgaggtcgtg aggtcgccgt tcttcaggat ctcgagcgtc tggaacttca cgtcgaccac
     2941 gcggtagtgg cggccgtgcg acagaccgat gaccggcagc tccagtggat cgcccgcgaa
     3001 ggcatcgacg cagagctcgg caagcacgtc gctgcgcacg aggaggtcga tgacgccgaa
     3061 catgcgcagc tggggatcgc ggacgacggc gccggcgatg atcggcaccc cggcacgcat
     3121 cgcctcccac gtggccgtcg cggcgtcgaa cgatcgcgcc tggtcgggcc gcgaggcgac
     3181 gcgcaggacc tcccagcgcg ccacgaggtc cgcgatgagc gcccgctcga agccccggcc
     3241 ctgctgcatg aggaatcgcg cgagatcgaa gcgctcgtcg tagccgccga ggtcgcgatc
     3301 tcgcgtgaat ccgtgcccgg tgccgtagag gtcgagccag tccacgagga gatcgctccg
     3361 gcaccagttg cgcgtgtccc ctgccgccac ccaatcgagc cagtcgaggt ccgtcttcgg
     3421 gtcggcggcg gtgccgtcgt cgcggacgat gatcgcgtcg cggccgaacg ggatgtcagc
     3481 cctccagcac gtcgaggagt ccgcggaagt ccggctcgac ccgcagtgcc ttcgcgtcgg
     3541 tcggcggcgg aacgttcccg cggttggccc agtacacgtc cattccaacg cggacggcgc
     3601 cgccaacgtc atgcgccgac ccggcgacga agagcacctc ggccggatcc agcgcgagtc
     3661 gctccagcgc gaggcggtag ggacgggggt cgggcttgta ggcgccggcc tcctccgcgg
     3721 tcaccacgag gtcgaaggtc cccgcccgtc gcgccgcgcg ctcgcccagc tcgcgcgagc
     3781 agttggtgac gacgaaccgg cggcggtcgc cgagacgctc gagcacgggc ggcacatcgg
     3841 gccagggcgc gaactcgccg aagcgcgcga ggaactcgtc ggccgttccg cgcggtatcc
     3901 cgaccgcggc ggcggactcg cgggtgatgt cggcatagga gcggtacggc tggcctcgca
     3961 ggaggccctg cgaggcggca tgccagcgca tgcccagcgc gcggtcgccc gcgaccgtcg
     4021 cccacagcga ccacgtgtcg agcagcgcgg tgagcaggtc gaagccgacg gcccggtagc
     4081 gcatggggaa cagggtacga gcctagaagc cggcgatgat cttctggccc tcggacccgt
     4141 tcacgaattc gaggaacgcg ctgatggccg gcttcggccg accggtgcgg gtcacgagca
     4201 gatacagcgg ccggcgcagc ttgtatgacc cgtccatgag tgtcgccttc gtcggcatga
     4261 cgccgtcgat ccccacgaga cggacaccct cggccttggg gtcgtccgat gagacgccga
     4321 tgatgccgat cgctccgcga tacgacgcga tggcctggcg catctgatcg ccgcccttga
     4381 tctcgaggac gctcggaccg tagaggggct tgcccttgaa cgccacgttc tcgaaggtcg
     4441 tgcgcgcgct cgaaccgatc tcgcgcacca tcggcatgat ggtgccgttc tgcacgccga
     4501 gcatcgacca gtcgacggtc ttgcccgtgt agatctcgac gagctgcgcg gtgctgaggt
     4561 acttgatcgg gttctgcgcg ttcaccgcga agccggtgcc ggcgaccccg atcagatgac
     4621 gctcgacgag cgccgactcg tcgttcgtga gctcgcggct ggccatcccg agatcgagcg
     4681 atccatctgc cgcgagcttc accgccgggt cagagccgag cgaggtgtcg atgtcgaatt
     4741 tcacgccggg atgcttcgcc gcgtaggcgt cggtgagcgc ggtcacgatc tcgatggacg
     4801 cgtcgccgcc gccgatcttg tacacgccgg cgagcgggtc gcgcgccggc gtggcggcgg
     4861 gtgcgcatga ggcgaggacg agggcgaacg cgaccagtgc gttccgcatc gaccgatcag
     4921 tctaacgtcc gcatgctttc atgttcggtc tctgcggtca tagaccgttg ctcgccgcgt
     4981 ccatgccgcg gctatcctcg gcgcgcatgt ccatggtccg cagcgactgg ctgcacgcga
     5041 agacggagcc cgtcgctcgg aacggaatgg tcaccgccga gcatcacctt gccgcggaag
     5101 cgggcgtcga ggtcatgcgc gacggcggca acgcggtcga tgccgcggtc gccgcggcct
     5161 tcgtgatggg cgtcgtcgag ccattcacca gcggtatcgg cggcatcgcc ggtctggtgg
     5221 tgcgtcgtgc ggacggctcc gtgacgagca tcgaaggcgg cacgcgtgcg ccgaaggcgg
     5281 cgcggcccga catgttccag ctcgtgggtg gcggcgcgcg cgcgggcatg tatgcgtggc
     5341 cggccgtcaa agacgacgcg aacatcgagg gtccgctgtc ggtcggcgta ccgggtcagg
     5401 tcgcggcgct ctgtctcgcg cacgagcgct acggccgcct tccccgctcg cgcgtgatgg
     5461 agccggcggt gcgcatcgct cgcgaaggct tcgagatcga ctggtacgtg agcctgtcgc
     5521 tggcgatgta tcacgagcgg ctgcatcgtt ccggtgacgg cggcgagatc ttcttccgcc
     5581 gctccggcgc cccgctgcgt cctccgatgg ggaccgagcc gtccgacagg ctcgtgcagc
     5641 ccgcgctcgc gacggcgctc gagacgatcg ctcgcgacgg gccacgcgtg ctgtacgagg
     5701 gcgagctcgg ccgcgcgatc gtcgagggcg tgcgcgcctg tggcggcgtg ctgtcgttcg
     5761 acgatctgcg cggctacgcg gcggcggaga ccgcgccgct cgcgacgatg taccgcggtc
     5821 atcgcgtcgc gacgctcccc ggcctcaccg gcgggccgac ggtggcgcgg acgctggcga
     5881 tcctcgaagc gttcccggtg ggcgcgaacg aacagggcgg cgcggagcat ctgcacctcg
     5941 tggcggaggc gcttcgcgcc gccttcctcc agcgcttctc gtcgctcgcc gaccacacca
     6001 cgcacatcaa cgccgtggat ggcgacagga cgctggtctc gctcacccag acgctcggcc
     6061 agggcttcgg ctccggcttc gtgccgaagg ggaccgggat cgtgctgctc gacacgatga
     6121 cctggttcga tccggtgccc gggcacccga actcgatcgg ccccgaaaag cgcgtgctct
     6181 gggccggcgc gccgaccatc gtgctgcgcg gccacgcacc gctcctcgcc gtgggcgcgc
     6241 ccgggggccg aaagatcatg agcgcggtcg cgcagtccat cgtgaacgtc gtcgatttcg
     6301 gcgacggtcc gctcgacgcg gtgaaccggc cacgcgtcca cgacgaaggc gagggcctcc
     6361 tcgtggacac gcgggtcccc gaggccgtgc gcgcggagct cgcggacatg gggcacgccg
     6421 tccagctgaa ggaggagacg ctcatgtcgg catggttcgc ccgtccccag gcgatcctcg
     6481 tcgacccgaa gaccggcgac ctgcgcggcg gcgtggacgc gctgaagccc gcggcggcca
     6541 tcgggtacta gggactaggc taggccgcgg atggcgcgtt tcatcgtcga gggcggcgtc
     6601 ccgctgcatg gcgagatcac gccgtccggg aacaagaacg aagcacttcc gctcatcgcc
     6661 tgcgccatgc tcaccgatga acccacgacg ctccgcaacg tg
//
LOCUS       DASTZR010000433         2854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_180268, whole genome shotgun sequence.
ACCESSION   DASTZR010000433 DASTZR010000000
VERSION     DASTZR010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180268"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VFV20_09635"
     CDS             <1..744
                     /locus_tag="VFV20_09635"
                     /inference="COORDINATES: protein motif:HMM:NF024763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEU5288654.1"
                     /translation="WKERFALHDDPQPDRDWPTHELAYADAAMQRTTDAVAFTLADLA
                     LCDVRQADDLARIPRERWNEHLVPIEKWLDAPANGHVPYVYAIDERAGLHRVLVDEHL
                     AALTRRCAQSWRRLRELDDLKRERAPQAAAAPVAAPEAVAVATASASAAAPAAAPAEA
                     PAAPSSDEAYIETPRCTTCNECTNLNPRMFAYNENKQAYIKDVSAGTYKDLVEAAENC
                     QVAIIHPGKPRDPNEPGLEDLLKRAEGFR"
     gene            complement(760..2217)
                     /locus_tag="VFV20_09640"
     CDS             complement(760..2217)
                     /locus_tag="VFV20_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288655.1"
                     /translation="MEAGLYVTFFDGGEAPERELPPVGPLEHVVLRHRELVAERRAVT
                     ETPDTGVDVARWLEAELELQRATGEEPGGPKRAEMRILARDGVYVRFAVFGDVRERDA
                     VAELGPYAVVVIGRRGVEADGQLLATRSSSDLAAWELTTHAGIEAAGRHKPDIAFRTP
                     TVPYHPQIATAPAQRGPAVAPATLPGAPPPPIVPVPTESAFELPPSPRAQETEPLFRP
                     ATPHDREIYTPPAYTPPAEEPSALTEADLALIQRIEQQRAEETLRARIQEEERRRLGV
                     DDSEDEASTWAIRYRTQPAQAEAPATAEAAGGGFTLGPLLWRLRFAIIGVLLLGVGAY
                     GFTLVRSGGASNVPGQPPQVKFVGIAQKVSSARWDYIVNGVQRATSAGTAAPRGVYYV
                     VRVGITNRGTDGAQLSPGEFVLIDATGTEHRAEPATGAPYYSPSNTQSQFIWPQSFPV
                     GRAATVSVIFDVDPGLGRGMQLAISDLPATRVKLD"
     gene            complement(2273..>2854)
                     /locus_tag="VFV20_09645"
     CDS             complement(2273..>2854)
                     /locus_tag="VFV20_09645"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU5288656.1"
                     /translation="VDVGEHVGDALDPMLDAFARVSRRLLETPVPTLAVVHGAALGGA
                     CEIVALCDLAITTDDAKLGTPEIALGVVPPVGAAIFPSLVGAQRARALVLLGEPVRGT
                     EAAASGLVWKSVAPDALDAEVAKITERFRSLSGASLRLAKRTFRVAELARTPVEAVLA
                     ADGEQRRSLPRMHDADEGLRAFLEKRAPRWLHR"
BASE COUNT          400 a         1041 c         1036 g          377 t
ORIGIN      
        1 tggaaggagc gcttcgcgct ccacgacgat ccgcagcccg accgcgactg gccgacccac
       61 gagctcgcct acgcggacgc cgcgatgcag cgcacgaccg acgcggtcgc cttcacgctc
      121 gccgatctcg cgctgtgcga cgttcgccaa gcggacgatc tcgcacggat cccgcgcgag
      181 cggtggaacg agcacctagt gccgatcgag aagtggctcg acgctcccgc caacgggcac
      241 gtgccgtacg tatacgcgat cgacgagcgc gccggcctgc accgcgtcct ggtggacgag
      301 catctcgccg ccctcacgcg acggtgcgca cagtcctggc gccggctgcg cgagctggac
      361 gatctgaagc gcgagcgcgc gccgcaggcg gcagcggcgc ctgtggccgc gcccgaggcc
      421 gtcgccgtgg ccaccgcgtc cgcatcggcg gcagcgcccg ccgcggcgcc ggccgaggca
      481 cccgctgcgc cgtcctccga cgaggcctac atcgagacgc cgcggtgcac gacctgcaac
      541 gagtgcacga acctcaaccc gcggatgttc gcgtacaacg aaaacaagca ggcgtacatc
      601 aaggacgtca gcgccgggac gtacaaggac ctcgtcgagg cggccgagaa ctgccaggtc
      661 gcgatcatcc accccggcaa gccgcgcgac ccgaacgagc ccgggctcga ggatctgctg
      721 aagcgcgcgg agggcttccg ctagcaggcg gcagggtcgc tagtcgagct tcacccgcgt
      781 cgcaggcagg tcactgatcg cgagctgcat ccctcggccg aggcccggat cgacgtcgaa
      841 gatgacgctc accgtagcgg cccttcccac cgggaacgac tgcggccaga tgaactgcga
      901 ctgcgtgttg ctcgggctgt agtacggcgc gccggtggcg ggttcggcgc gatgctcggt
      961 gcccgtcgcg tcgatgagca cgaactcgcc cggtgagagc tgcgctccat cggtgccgcg
     1021 attggtgatg cccacgcgca cgacgtagta cacgccgcgc ggcgcggccg tgcccgcgct
     1081 cgtcgcgcgc tggacgccgt tcacgatgta gtcccacctg gcgctcgaca ccttctgcgc
     1141 gatgccgacg aacttcacct gcggtggctg gcccggcacg ttcgaggcgc cgccgctgcg
     1201 cacgagggtg aagccgtagg ccccgacccc gagcagtagc acgccgatga tcgcgaatcg
     1261 caggcgccag agcagcggac cgagcgtgaa gccgccaccc gctgcctccg cggtcgccgg
     1321 tgcctccgcc tgggcgggct gcgtgcggta gcggatcgcc caggtgctgg cctcgtcctc
     1381 gctgtcgtcg acgccgagcc gccggcgctc ctcctcctgg atgcgcgctc tgagcgtttc
     1441 ttcggctcgc tgctgctcga tgcgctggat gagcgcgaga tccgcctcgg tgagcgccga
     1501 tggctcctcc gcgggcggtg tgtacgccgg cggcgtgtag atctcgcggt cgtgcggggt
     1561 cgcgggccgg aagagcggct ccgtctcctg cgcccgcgga gagggtggga gctcgaacgc
     1621 ggactcggtc ggcaccggga cgatgggcgg cggcggcgcg cccggtagcg tggcgggcgc
     1681 gacggccggg cctctctgag cgggcgcggt cgcgatctgc gggtgatagg ggaccgtcgg
     1741 cgtgcggaac gcgatgtccg gcttgtgccg gccggcggcc tcgatccccg cgtgcgtcgt
     1801 cagctcccac gccgcgaggt cactcgagga tcgtgtggcg agcagctggc cgtcagcctc
     1861 gacgccgcgg cggccgatga cgacgaccgc gtacggtccg agctcggcga cggcgtcccg
     1921 ctcgcgtacg tcgccgaaga ccgcgaagcg cacgtacacg ccgtcgcgcg cgaggatccg
     1981 catctccgcg cgcttggggc cgcccggctc ctcgcccgtc gcgcgctgca gctcgagctc
     2041 tgcctcgagc caccgcgcga cgtcgacgcc cgtgtcgggc gtctccgtga ccgcgcggcg
     2101 ctcggcgacc agctcacggt gccgcaggac gacgtgctcg agcggaccga cgggcggcag
     2161 ctcgcgctcg ggcgcctcac cgccatcgaa gaacgtcacg taaaggccgg cttccactgc
     2221 cgcgaagcct aacggcgtga agggtcgccg agtacccgcg agcgagggag gtctagcggt
     2281 gcagccagcg gggcgcgcgc ttctcgagga acgcgcgaag gccctcgtcc gcgtcgtgca
     2341 tgcgcgggag cgagcgccgt tgctccccat ccgcggccag cacggcctcg acgggtgtgc
     2401 gtgcgagctc ggccacgcgg aaggtgcgct tcgcgaggcg caggctcgca cccgacagcg
     2461 accggaaccg ctcggtgatc ttcgccacct cggcgtcgag cgcatcgggc gcgacgctct
     2521 tccagaccag gccgctggcc gccgcctcgg tgccgcgcac cggttcgccg agcagcacga
     2581 gcgcccgggc ccgctgcgct ccgacgagac tcgggaagat cgccgctccg acgggcggca
     2641 cgacgccgag cgcgatctca ggggtgccga gcttcgcgtc gtcggtcgtg atggcgaggt
     2701 cgcagagcgc gacgatctca catgctccgc cgagcgccgc gccgtggacc accgcgagcg
     2761 tcggcaccgg ggtctcgagg aggcgccgcg acacgcgggc gaatgcgtcg agcatcggat
     2821 cgagcgcgtc gccgacgtgc tcgccgacgt cgac
//
LOCUS       DASTZR010000434         3816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_24074, whole genome shotgun sequence.
ACCESSION   DASTZR010000434 DASTZR010000000
VERSION     DASTZR010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24074"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1354)
                     /locus_tag="VFV20_09650"
     CDS             complement(<1..1354)
                     /locus_tag="VFV20_09650"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEU5288657.1"
                     /translation="MQKNRLTWMIGGPQGSGINASAEVFAKACSRAGLRVYANIEYHS
                     NIMGKHSFYRVRVDDEDIRSYRDRTDILVALDHETLAGDGDHRRWATHFGHLQELNDG
                     GGVVYDSEVEFDTTEAQRKDLRFYPVPFMEIIKKALEEVGKGEQARRYEVMKNTVGLG
                     ASLALCDYPFDLVAEVIQGQFKGKRSEIGQLNVRAARLAFDHVKQNFDIAGFPYTLRP
                     GPEPKARILAKGYEIAAIAKLRAGCAMQTYYPISPATDESVYLERQQREYGLLVVQCE
                     DEISSINMAVGAAHMGVRVATATSGPGFALMVEGIGFASITEAPGPVLVLYQRGGPST
                     GLPTRQEQGDLLFALHPAQGDFPHVVTAPGDLRETYQDVFSAFNWAERYQLPVIVLSD
                     KKLAAVYTTIDKLELSHPEIDRGLRFTGSEWTPVDAKGPNDGSAADGNGAAKEYLRYA
                     L"
     gene            1412..2821
                     /locus_tag="VFV20_09655"
     CDS             1412..2821
                     /locus_tag="VFV20_09655"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEU5288658.1"
                     /translation="MDARNASTRREKDTLGEVAVPETALWGAQTQRAIENYPISGYRA
                     FPAFTHAMTRIKKAEAIANAEVKRLDAAKKDAIVKACDEVLAGQHADQFVVDVFQSGA
                     GVSFHMNVNEVIANRANQILGGGLGTYDQVNPNDHVNMGQSTNDVTPTAMRLAHLELT
                     KNVCDQLDRLADALDAKAKQYANVVKPGRTHLQDAVPITFGQEIGGWATRVRGASARL
                     RAGRGELCELGIGGTAAGTGLNATPGVRERVCQLLAQWYDEPIVPAKDLFASMQSMAP
                     FIRISSGMRTAAVEVSQMCNDIRLLVSGPRTGFGELIIPAVQPGSSIMPGKVNPAIVE
                     MVNQVCFQVIGNDTAVVWAAQAGQLDLNVMMPEVNFAVCFSATIFANAVKQLRERTVE
                     GMRVDEARCKELMDLSPSLIVTALSPHIGYAKSAALVKKALAERRSLMDVALEEKVMD
                     REKLEKVLDPLPMTKGGVQ"
     gene            2890..3633
                     /locus_tag="VFV20_09660"
     CDS             2890..3633
                     /locus_tag="VFV20_09660"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5288659.1"
                     /translation="MKQVSALPWVREYKRRTFALLDLRPGLHVLDVGCGRGDDVIALA
                     KIVGPNGRVAGVDQSAVMLAAARTAAHDAGVAVELVEADAASLPFPDESFDACRIDRT
                     LEHLQDPAAALRELVRVCRPGGRVATAEPDWDTLAVDAPDLETSRAVTRAQSAEIAHG
                     TIGRSLRRLLIEAGLRDVVTDTMALAFEDLTTAEAVYGIRHALAKVVKGREVEPARAD
                     AWWRSLEEADAAGTFWASMIGTIAIGRKP"
BASE COUNT          603 a         1282 c         1344 g          587 t
ORIGIN      
        1 tgagcgcgta gcggaggtat tccttcgccg cgccgttccc gtccgccgcg ctgccgtcgt
       61 tcgggccctt cgcgtcgacc ggtgtccact cgctgccggt gaagcggagg ccgcggtcga
      121 tctcggggtg cgagagctcg agcttgtcga tcgtcgtgta caccgcggcg agcttcttgt
      181 ccgacagcac gatgaccggg agctggtagc gctcggccca gttgaacgcg gagaacacgt
      241 cctggtaggt ctcgcgcaga tcgcccggcg cggtgacgac atgcgggaag tcgccctgcg
      301 cggggtgcag cgcgaagagc aggtcgccct gctcctgccg cgtcggcagc ccggtggagg
      361 ggccgccgcg ctggtagagc acgaggaccg ggcccggcgc ctcggtgatg gaggcgaagc
      421 cgatgccctc gaccatgagc gcgaagccgg ggccgctggt ggccgtcgcg acgcgcaccc
      481 ccatgtgggc ggcgccgacg gccatgttga tcgacgagat ctcgtcctcg cactgcacga
      541 cgaggaggcc gtactcgcgc tgctggcgct cgaggtacac gctctcgtcg gtcgcgggcg
      601 agatggggta gtaggtctgc atcgcgcagc cggcccggag cttggcgatg gcggcgatct
      661 cgtagccctt ggcgaggatg cgcgccttcg gctcgggacc cggccggagc gtgtatggga
      721 agccggcgat gtcgaagttc tgcttcacgt gatcgaacgc gagccgcgcg gcgcggacgt
      781 tgagctgtcc gatctcggag cgcttgccct tgaactgtcc ctggatgacc tccgcgacca
      841 gatcgaaggg gtaatcgcac agcgcgagcg acgcaccgag gccgaccgtg ttcttcatga
      901 cctcgtagcg ccgcgcctgc tcgcccttgc cgacctcctc gagcgccttc ttgatgatct
      961 ccataaaggg caccggatag aagcggaggt ccttgcgctg cgcctcggtc gtgtcgaact
     1021 cgacctcgct gtcgtagacg accccgccgc cgtcgttcag ctcctgcaga tggccgaagt
     1081 gcgtggccca gcgtcggtga tcgccgtcgc cggcgagcgt ctcgtggtcg agagccacca
     1141 ggatgtcggt cctgtcgcgg tatgagcgga tgtcctcgtc atcgacgcgc acccggtaga
     1201 acgagtgctt gcccatgatg ttggagtggt actcgatgtt cgcgtagaca cgaagacccg
     1261 ctcgggagca ggccttggcg aacacctcgg cggaggcgtt gataccgctg ccctggggtc
     1321 cgccgatcat ccaggtcagc cgattcttct gcacgcggaa cacctattgt aaccagcacc
     1381 gggacggagg agttgcctta tggcaggcgc catggacgca cgtaacgcct ccacgcgacg
     1441 cgagaaggac accctgggcg aggtcgctgt gcctgaaacg gcgctctggg gcgcgcagac
     1501 gcagcgcgcg atcgagaact acccgatcag cggctaccgc gcgtttcccg cgttcacaca
     1561 cgcgatgacc cggatcaaga aggccgaggc gatcgccaac gccgaggtga agcggctcga
     1621 tgccgcgaag aaggacgcca tcgtcaaggc ctgcgacgag gtgctcgctg gccagcacgc
     1681 cgaccagttc gtcgtcgacg tgttccagtc cggcgcgggc gtctccttcc acatgaacgt
     1741 gaacgaggtg atcgcgaacc gcgcgaatca gatcctcggc ggcgggctcg gcacctacga
     1801 ccaggtgaat ccgaacgacc acgtgaacat gggccagtcc acgaatgacg tgacgcccac
     1861 ggccatgcgc ctcgcgcacc tcgagctcac gaagaacgtc tgcgaccagc tcgaccgcct
     1921 cgccgacgcg ctggacgcga aggcgaagca gtacgcgaac gtcgtcaagc cggggcgcac
     1981 gcacctgcag gacgcggtgc cgatcacgtt cggtcaggag atcggcggct gggccacccg
     2041 cgtgcgaggc gcctcggcgc gcctgcgggc cggccgcggc gagctctgcg agctcggcat
     2101 cggcggcacc gcggcgggca ccggtctcaa cgcgacgccc ggcgtgcgcg agcgggtatg
     2161 ccagctgctg gcgcagtggt acgacgagcc catcgttccg gcgaaggacc tcttcgcgtc
     2221 gatgcagtcg atggcgccgt tcatccgcat ctcgagcggc atgcgtaccg ccgcggtcga
     2281 ggtctcgcag atgtgcaacg acatccgcct cctggtgtcc ggaccgcgga ccggattcgg
     2341 cgagctcatc atcccggcag tgcagccggg ttcctcgatc atgccgggca aggtgaatcc
     2401 cgcgatcgtg gagatggtca accaggtctg cttccaagtg atcggcaacg acactgccgt
     2461 cgtgtgggcg gcgcaggccg gacagctcga cctcaacgtc atgatgcccg aggtgaactt
     2521 cgcggtctgc ttctccgcca cgatcttcgc gaacgcggtg aagcagctgc gcgagcggac
     2581 cgtcgagggc atgcgcgtcg acgaggcgcg ctgcaaggag ctgatggacc tctcaccgtc
     2641 gctcatcgtg acggccctct cgccgcacat cggctacgcg aagtccgcgg cgctggtcaa
     2701 gaaggcgctc gccgagaggc ggtcactcat ggacgtcgcg ctcgaggaga aggtgatgga
     2761 cagggagaag ctcgagaagg tcctcgatcc actgcccatg accaagggcg gggtgcagta
     2821 acgcaggccc cgcgtcgcgg ctgggcgaag gtcgacgagg ccaccgacca ggcgtacttc
     2881 ctcgactatc tgaaacaggt cagcgcgctg ccgtgggtgc gggagtacaa gcggcggaca
     2941 ttcgcgctgc tcgacctccg tcccggactg cacgtcctcg acgtcggctg cgggcgcggc
     3001 gacgacgtca tcgcgctcgc gaagatcgtc ggtccgaacg gccgtgtcgc cggggtcgat
     3061 cagagcgcgg tgatgctcgc ggcggcgcgc acggcggcgc acgacgccgg cgtcgctgtc
     3121 gagctcgtcg aggcggatgc cgcgtccctg cccttcccgg acgagagctt cgacgcctgc
     3181 cgcatcgatc ggaccctcga gcacctgcag gaccccgcgg ccgcgctgcg cgagctggtg
     3241 cgggtatgcc gtcccggtgg acgggtcgcc accgccgagc ccgactggga cacgctcgcg
     3301 gtcgacgcgc cggacctcga gaccagccgc gccgtcacgc gcgcgcagtc cgccgagatc
     3361 gcgcacggga cgatcggccg gtcgctccgg cgcttgctca tcgaagccgg gctccgcgac
     3421 gtcgtcaccg acacgatggc cctcgccttc gaggacctga ccaccgccga ggccgtgtac
     3481 ggcatccgcc acgcgctcgc gaaggtcgtg aaggggcgtg aggtcgagcc cgcgcgcgcc
     3541 gacgcctggt ggcgctcgct cgaggaagcg gacgcggccg gcacgttctg ggcgagcatg
     3601 atcgggacga tcgccatcgg acggaagcca tgagcatccg gctcgcgcaa cgtgaggtgc
     3661 agagtggctg agatcgccgt ggcctttccg ctggatgagg cgaccgccgg cgtcatcgtg
     3721 tcgcggctcg ccgtcgacgg catcgccgcc cggatcgatc gcgggctgta cggcgcgttc
     3781 ctcgcgccgc agcagggcca gctgcgggtc atggtc
//
LOCUS       DASTZR010000435         4114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_964151, whole genome shotgun sequence.
ACCESSION   DASTZR010000435 DASTZR010000000
VERSION     DASTZR010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964151"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VFV20_09665"
     CDS             <1..335
                     /locus_tag="VFV20_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010497170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEU5288660.1"
                     /translation="GVTTLTMADIDRLGMAAAMEQAIAVAGSDGPIHLSLDMDALDPN
                     EAPGVGTPVRGGLTYREAQLAMEMLAASGKVGSIEIAEVNPILDTGNRTASLAVELVA
                     SALGQTIL"
     gene            complement(372..1049)
                     /locus_tag="VFV20_09670"
     CDS             complement(372..1049)
                     /locus_tag="VFV20_09670"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEU5288661.1"
                     /translation="MPSDFLDAVKRGDRQTVEQMVRADPGLLASKDENGTSALLLAYY
                     HGKAEIANALLTHRPDLDIFEAATVGDAARIRELAARDPSLVNAVAGDGFFPLGLAAF
                     FKRPDAVRALLELGADVRPASRSGGFTPLHSAVADDTGTVAKEIVRMLLDAGADPNAK
                     SAGGGTPLHTAAFVGDVEITRLLLAAGADPQVADAKGRTALDVAREKDRTEAAALLHD
                     AVLGKRA"
     gene            1100..1534
                     /locus_tag="VFV20_09675"
     CDS             1100..1534
                     /locus_tag="VFV20_09675"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU5288662.1"
                     /translation="MSRTASRSERETADRLHSAAIHLLRRVRRTDALTGLPAAQLSAL
                     SVLMGGPRSLGEMAAAEQVRPPTMSKLVTEMEAAGLVQRSSDRQDARVVRVGMTARGR
                     RLLAKGRDMRIADVERRLRAQPPKDVELVTNALAVIERMLQD"
     gene            1602..3755
                     /gene="cysS"
                     /locus_tag="VFV20_09680"
     CDS             1602..3755
                     /gene="cysS"
                     /locus_tag="VFV20_09680"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU5288663.1"
                     /translation="MQLRLFNTLTRRIEPFVPLNRGEVRMYTCGPTVYRPVHLGNLRS
                     YLLADWLRRAFELEGWRVVQVKNVTDVGHMRQDAVDRGEDKVIAAALAEGRTPMEIAA
                     TYEARFREDERALGILPATVYPRATEHVADMVAMVERLVSLGFAYVVDGTVYYSVKSF
                     AGYGRLSGNVQEALRQGVRAEIDPAKRDPSDFALWKRAERGRQALVWESPWGSGYPGW
                     HIECSAMSVRYLGDRFDLHTGGVDNIFPHHEDEIAQSEGALGHPVVGTWVHGQHLLAD
                     GVKMAKSAQNTAEITELIGLGIDPLAFRYQCLLTHYRARMEFTHAALRQAAEGLDHLR
                     QRMRTLAQLWPEATATDARTSWVARFRERVADDLDLPGALAVAHACVTDAATSARDRL
                     EAVQVMDRVLGLELGSAAREIEELPHHIRHIVDERRSARASRDLRVADELRTGLDGWR
                     IDDLRDGSVIVSRAERRIAPRTLRRISSGRQLRDRRAEEPTRSWSVCVVTREFPDDVE
                     RTIASVLRWLPADGEIVVLDQGSSTETGRRLLALASDRVGIVLADRDLGEGAARNALL
                     RIARGRLLLQLDPSVELTDDLFTPLAAALRDSAAGAAGPWGLRAVDHLKQWDEVTAGP
                     CDAIQGYCFATRREALLAAGGFDERYRFYRNLDIAVSLALRARGLRCVAVGDTRVARH
                     AHRAWERLAEDERQKRSRRNYDRLVRRFGSLGVGT"
     gene            3752..>4114
                     /locus_tag="VFV20_09685"
     CDS             3752..>4114
                     /locus_tag="VFV20_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEU5288664.1"
                     /translation="MSGRLVLCGSGEFTTAMDALDTELLAGLRPRAKVAIVPTAAGLE
                     DTPARWGEMGCAHFRALGAEAVPLMVLDRRDANEPRWTDALADVDWIYFSGGDPGHLV
                     ETLEGSAFWTAVLARHRHG"
BASE COUNT          559 a         1490 c         1476 g          589 t
ORIGIN      
        1 ccggtgtgac cacgctcacg atggccgaca tcgatcgcct cggcatggcc gcggcgatgg
       61 agcaggcgat cgccgtcgcg ggcagcgacg gtccgatcca tctctccctg gacatggacg
      121 cgctcgatcc gaacgaggcc cccggcgtcg gaacgcccgt ccgcggcggg ctcacgtaca
      181 gggaggcgca gctcgcgatg gagatgctcg cggcgtcggg caaggtgggg tccatcgaga
      241 tcgccgaggt gaacccgatc cttgacacgg gcaaccgcac ggcgtcgctc gccgtcgagc
      301 tcgtcgccag cgcgctgggc cagacgatcc tctagtcggc gggtgcctgc cgcaatgccc
      361 ggcgccgctc cctacgcgcg cttacccagg acggcgtcgt gcaggagcgc ggccgcctcg
      421 gtgcgatcct tctcgcgcgc gacgtcgagc gccgtgcgcc ccttcgcgtc cgcgacctgc
      481 gggtcggcgc cggcggccag cagcagcctc gtgatctcga cgtccccgac gaacgcggcc
      541 gtgtgcagcg gcgtgccgcc accggcgctc tttgcgttgg gatcggcacc cgcgtcgagc
      601 agcatgcgca cgatctcctt ggcgacggtc ccggtgtcgt cggccacggc cgagtgcagc
      661 ggcgtgaacc cgccgctccg tgaagccgga cgcacgtcgg cgccgagctc gagcagcgcg
      721 cgcacggcgt cgggacgctt gaagaacgcc gccagcccca gcgggaagaa gccgtcgccc
      781 gcgaccgcgt tcacgagcga cggatcgcgc gcggcgagct cgcggatccg cgccgcgtcg
      841 ccgacggtcg ccgcctcgaa gatgtcgagg tccggccggt gggtgagcag cgcgttcgcg
      901 atctccgcct tgccgtggta gtaggcgagc aggagcgccg aggtgccgtt ctcgtccttc
      961 gacgcgagca ggcccggatc agctcgcacc atctgctcga cggtctgccg gtcgccgcgc
     1021 ttcaccgcgt cgaggaagtc gcttggcatc gccgcctccc cacttgacga tagttaggtt
     1081 cgctaactaa tatcacgccg tgtcaaggac cgcaagccgc agcgagcgcg agaccgccga
     1141 ccggctccat tcggcggcga tccacctgct gcggcgggtg cgccgcacgg acgcgctcac
     1201 cggtctgccg gccgcgcagc tgtcggcgct gtccgtcctg atgggcggcc cgcgctcgct
     1261 cggcgagatg gccgcggcgg agcaggtgcg tccgccgacg atgagcaagc tcgtgaccga
     1321 gatggaggcg gcgggcctgg tccagcgctc ctccgatcgt caggacgcgc gcgtcgtgcg
     1381 cgtcggcatg accgccagag ggcgccggct gctcgcgaag gggcgcgaca tgcgcatcgc
     1441 ggacgtcgag cgccgactgc gcgcgcagcc gccaaaggat gtcgagctcg tgacgaacgc
     1501 gctggcggtc atcgaacgga tgctgcagga ctagcgcgcc tctcccgcat ctcgtcgcgc
     1561 ggcagacatc agcggtcgta ccctgttccg caggggagac cgtgcagctt cgtctattca
     1621 acacgctcac acggcggatc gagcccttcg ttccgctgaa caggggcgag gtccgcatgt
     1681 acacgtgcgg gcccaccgtg taccgaccgg tgcacctcgg gaatctgcgc tcctatctcc
     1741 tcgccgattg gctgcgccgc gcgttcgagc tcgaaggctg gcgcgtggtg caggtgaaga
     1801 acgtcaccga cgtgggccac atgcgccagg acgcggtcga ccgcggcgag gacaaggtga
     1861 tcgcggccgc gctcgccgag ggcaggacgc cgatggagat cgcagccacg tacgaggctc
     1921 gcttccgcga ggacgagcgc gccctcggca tacttccggc gacggtgtac ccgcgtgcca
     1981 ccgagcacgt cgccgacatg gtcgcgatgg tcgagcggct ggtctcgctc gggttcgcgt
     2041 acgtcgtgga tggcaccgtc tactactcgg tgaagagctt cgcgggctac ggccggctct
     2101 cgggcaacgt ccaggaggcg ctccgccagg gcgtgcgcgc cgagatcgac cccgcgaagc
     2161 gcgatccctc ggacttcgcg ctctggaagc gcgcggagcg cggccggcag gcgctggtgt
     2221 gggaatcgcc gtggggcagc ggctacccgg gctggcacat cgagtgttcg gcgatgagcg
     2281 tccgctacct tggcgaccgc ttcgatctgc acaccggcgg cgtcgacaac atcttcccgc
     2341 accacgagga cgagatcgcg cagtccgagg gcgccctggg ccacccggtc gtcggcacgt
     2401 gggtgcacgg gcagcacctg ctcgccgacg gcgtgaagat ggcgaagtcg gcgcagaaca
     2461 ccgccgagat caccgagctc atcggtctcg gtatcgaccc gctcgcgttc cgctatcagt
     2521 gcctcctcac gcactaccgc gctcgcatgg agttcaccca cgccgcgctc cgccaggcgg
     2581 cggaggggct cgaccatctg cgccagcgca tgcgtacgct cgcgcagctg tggcccgagg
     2641 cgaccgccac ggacgcgcgc acgtcgtggg tcgcgcgctt ccgcgagcgg gtcgccgacg
     2701 acctcgatct cccgggagcg ctcgccgtcg cgcacgcctg tgtcaccgac gcggccacga
     2761 gcgcccgcga ccgcctcgag gcggtccagg tcatggaccg cgtccttggc ctggagctcg
     2821 gttccgcggc gcgcgagatc gaggagctgc cgcaccacat ccgccacatc gtcgacgagc
     2881 ggcgcagcgc gcgcgcatcg cgcgatctgc gcgtcgccga cgagctgcgc accggcctcg
     2941 acggctggcg catcgacgac ctccgcgacg gctcggtcat cgtctcccgt gccgagcggc
     3001 gcatcgcccc gcgcacgctg cgccgcatct cgtcgggtcg gcagctgcgc gaccggcgcg
     3061 cggaggagcc cacccggtcg tggtcggtgt gcgtggtcac gcgcgagttc cccgacgacg
     3121 tcgagcgaac catcgcgtcc gtgctgcgct ggctcccagc ggacggcgag atcgtggtcc
     3181 tcgatcaggg ctcatcgacc gagaccggcc gccgtcttct cgcgctggcg tccgaccgcg
     3241 tcggcatcgt gctcgcggac cgcgatctcg gcgagggtgc ggcgcgcaac gcgctgctgc
     3301 ggatcgcgcg cggccgtctc ctcctgcagc tcgatccctc ggtcgagctc acggacgatc
     3361 tcttcacccc gctcgcggcg gcgctccgcg acagcgccgc cggtgccgcc ggtccatggg
     3421 gcctccgtgc ggtggaccac ctgaagcagt gggatgaggt gaccgcgggt ccgtgcgacg
     3481 cgatccaggg gtactgcttc gcgacgcggc gcgaggcgct cctcgcggcc ggcggcttcg
     3541 acgagcggta ccgcttctac cggaacctgg acatcgcggt ctcgctcgcg ctgcgcgcgc
     3601 gggggcttcg ctgcgtcgcg gtcggtgaca cgcgggtggc gcggcacgcc caccgcgcgt
     3661 gggagcggct cgcggaggac gagcgccaga aacgcagccg gcgcaactac gaccggctgg
     3721 tgcggcgctt cggctctctc ggggtgggca cgtgagcggc cgcctcgtgc tctgcggatc
     3781 gggcgaattc acgacggcga tggacgctct cgacacggag ctcctcgccg gactccgtcc
     3841 gcgggccaag gtcgcgatcg tgccgaccgc ggccgggctc gaggacaccc ccgcacgctg
     3901 gggcgagatg ggatgcgcgc acttccgcgc cctcggcgcg gaagcggtgc cgctgatggt
     3961 cctggaccgg cgcgacgcga acgagccccg ctggaccgat gccctggccg acgtcgattg
     4021 gatctacttc tccggcggcg accccgggca cctggtcgag accctcgagg gatcggcgtt
     4081 ctggaccgcg gtgctcgcgc ggcaccgcca cgga
//
LOCUS       DASTZR010000436         7250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_598649, whole genome shotgun sequence.
ACCESSION   DASTZR010000436 DASTZR010000000
VERSION     DASTZR010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7250
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598649"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..1214)
                     /locus_tag="VFV20_09690"
     CDS             complement(78..1214)
                     /locus_tag="VFV20_09690"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEU5288665.1"
                     /translation="MTTEPKPQASPRGLEGVVATSTAICKVEDNRLIYRGYPIEELAT
                     KAEYEEVAYLLLNGDLPTKEQLRDFKAELTKHRALSPFLRQILQEMSENGTAMDVLRT
                     VVSAAATEDPAEGDNSRDAEYQKAVRLTAKLPTILATYIRRRKGQQPVPPDPKLGHAA
                     NFARMAGLDEAHPRAAEILNTCFILQAEHGLNASTFTARIVAATDADMHASVTAAFGA
                     LKGPLHGGATDGTMKMLDEIGSPEHVDAWVDKAIERKYKFSGFGHRVYKTEDPRAKYL
                     RRFASELQKDWDGPKYFDIQERLAQAIAERRPKMSPAAAAKVNIVNVDFFAATTYTFL
                     GIPADLGTSLFSIGRIAGWAAHIMEQHGDNRLIRPESTYTGPEG"
     gene            1586..2563
                     /locus_tag="VFV20_09695"
     CDS             1586..2563
                     /locus_tag="VFV20_09695"
                     /inference="COORDINATES: protein motif:HMM:NF020700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288666.1"
                     /translation="MDGAVLVLAGAFLIFFAGPSLGGALGDGAEDIRAQVSAMFPTLQ
                     GTGSIQLPSGGGTVAADPVANGLPDFTREPALTVNGRVPTFAVTPGRSVQVALNGALV
                     ATVTPDASGAFTTALTLREGPNAITLTLLGTADIVAHSSYTVVLDRQPPTLAVSKPAN
                     GETVEGPNVAVQGKVEAGATVIVNDRIVLTAPDGSFSESFGASPGPLPITVTARDRAG
                     NETTVKTAITVAAPVTTAPLAVNVTLDKIKVTPGAWVIAAIRVTANGTPRSGEQVTLS
                     VGVITIGSATTDPSGVARIGFAAPPNEGDAAVVVLANGATGRATLTVAK"
     gene            2586..4217
                     /locus_tag="VFV20_09700"
     CDS             2586..4217
                     /locus_tag="VFV20_09700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEU5288667.1"
                     /translation="MTSADLVLFSILGGTALLLYGVRLVGEGLQRAAGTRLRHLLSTL
                     TGNRLKALLVGAGVTAVLQSSSATTVMLVGFASAGLLSLRQTIGVILGADIGTTVTVQ
                     LLAFDLLAVSPLIVFIGWMLWATGRGTPKYVGQAILGFGFLFLGMKLVADGTAPLARS
                     ALFADVLAALADQPLILLIISAAFSGLVRSSAATIGFALSLAAAGLLPLAGAIPIIFG
                     ANIGTAVTAILAAIGQNAEARRVAAAHAVFKVVGVALFFPFVTPFAELVSRTAPDVPR
                     QIANAHTIFNVAVAAIFLPASSWAAGFFTRTIADRASAPTGAIYLNAQTLDTPAVALG
                     QALRETLRMGDVVVRSLRDSIVVLERNDDALMRQVIARDDVIDGLEEDIKQFLIKLSA
                     HALTEEQAERETALIFVIANLEEIGDVIEKNLMELAEKKIRGGHVFSQQGWHEIAGLH
                     AKVVENVELALSALAANDADVAEKVIRHKTRVNLMERQFRQTHIQRLHEGLRESIDTS
                     SLHLDLLANLKRANSLAAGIAYAVLGRHAVTDDDA"
     gene            4210..4926
                     /locus_tag="VFV20_09705"
     CDS             4210..4926
                     /locus_tag="VFV20_09705"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEU5288668.1"
                     /translation="MPRAYVGTSGWSYTTWRPKFYPEGTKSKDFLAYYATKFRTIEIN
                     YTFNHLPTEKNVAEWTAATGDDFVFGLKASQQITHRAQLREPAETLPVFFERARLLGA
                     RLGPVLFQTPPWVKRDDDRLAMFLAALPRDVRCVLEVRDASWYADPVYELLRTANVAL
                     VHAEGEGHPSPVETLGATATFAYARLRAREGYPDAAVDAWAERLRAIIAAGKDVYAYF
                     RHDDDGSNALSAMRLRDALA"
     gene            4923..5492
                     /locus_tag="VFV20_09710"
     CDS             4923..5492
                     /locus_tag="VFV20_09710"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEU5288669.1"
                     /translation="MILGESVSAIIIASFAAFMAVAQVSRARAIARRGPSLRRAGGIA
                     DLATYFFWLPYLVVWLRPGPTLVLHEALGWLGLALAVGGVVFAVAAVATLGRHYDLKL
                     ELHAGHEIVRRGPYGVVRHPIYTGLAIHSLGACLATGNLIFIAGTLAITFPVFVARAR
                     AEERLLREEFGAEYDRYAREVPMLVPGLR"
     gene            complement(5489..6622)
                     /locus_tag="VFV20_09715"
     CDS             complement(5489..6622)
                     /locus_tag="VFV20_09715"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU5288670.1"
                     /translation="MESAVAVLEIGLVLLVAGVAGVLARRVGLPAVVGYLVVGLAVGP
                     FTPGYVADRRQIQLLADVGVVLLLFEVGIELDLRSLAREPRGILVAVPLQIAIVTAIG
                     AAVAVALGTGVLGAVTLGLVAALSSSVVVVNISRSRKRTTDRDTERALVVWAILQDAV
                     TLVAATILAVLLAPGDESPAVALARTLAFVMLAALVQGWVVPRLLGAVKDQPDSFLIA
                     AVSAALAIAGLGAVVFGVPVALAAFVAGTLFSVRPIAREARREVLPFRDLFAVLFFVA
                     IGTLVDPAAVAREPGWLVAFVALVALKAVVITVLAAVFRVPARHWQLGVGLAQIGEFS
                     FVVLGLGVAVGAVSAGQSSAALAAAALTIALSAVGARLFPKPA"
     gene            complement(6640..>7250)
                     /locus_tag="VFV20_09720"
     CDS             complement(6640..>7250)
                     /locus_tag="VFV20_09720"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEU5288671.1"
                     /translation="HHVAYVVVEVNAEPLARLRREGRPFVEGDAASEDVLKSAGIAHA
                     KGLLAVVDSDERVVYITLAARALNPGLYITARAGQPESPRRRELAGANRVVSPYRMAG
                     RLMAQLAIRPAVVDVIDTMQHGGSAIGIEEILVGATCAARGRTLREAGLLDAGHARLL
                     AVRRRDGTLHVNPDETLRIDDGDLVVAMGSETDLTTTAAVLQ"
BASE COUNT         1037 a         2596 c         2544 g         1073 t
ORIGIN      
        1 gctcgcccgg gagcgcgagc agggccgcca gcgctgcggt tgacgggggg ctaccgctcg
       61 gcgatcggga tgtagcgcta gccctcgggt ccggtgtacg tcgactccgg ccggatgagc
      121 cggttgtcgc cgtgctgctc catgatgtgc gcggcccagc cggcgatgcg ccctatggag
      181 aacagcgacg tgccgaggtc ggccgggatg ccgaggaacg tgtacgtcgt cgcggcgaag
      241 aagtcgacgt tgacgatgtt caccttcgcc gccgcggcag gcgacatctt cgggcgccgc
      301 tcggcgatcg cctgtgccag ccgctcctgg atgtcgaagt acttcggtcc gtcccagtcc
      361 ttttggagct cggacgcgaa gcggcgcagg tacttggcgc gcgggtcctc ggtcttgtag
      421 acgcggtggc cgaagccgga gaacttgtac ttgcgctcga tggccttgtc gacccacgcg
      481 tcgacatgct cgggcgatcc gatctcatcg agcatcttca tcgtgccgtc ggtggcgccg
      541 ccgtgcagcg gtcccttcag cgcgccgaag gcggcggtga cggaggcgtg catatccgcg
      601 tcggtcgccg cgacgatgcg cgcggtgaac gtcgacgcgt tcagtccgtg ctcggcctgg
      661 aggatgaagc aggtgttgag gatctccgcc gcgcgcgggt gcgcctcgtc gagtccggcc
      721 atgcgcgcga agttcgctgc gtggccgagc ttcgggtccg gcggcacggg ctgctggccc
      781 ttccggcggc ggatatacgt cgcgaggatc gtgggcagct tcgccgtcag gcgcacggcc
      841 ttctggtact ccgcatcgcg ggagttgtcc ccctcggcgg gatcctccgt cgccgcggcg
      901 gacacgacgg tgcgcaggac gtccatggcc gtgccgttct cggacatctc ctggaggatc
      961 tggcggagga acggggacag ggcacggtgc ttcgtcagtt cggccttgaa gtcgcggagc
     1021 tgctccttcg tggggaggtc gccattgagc aggaggtacg cgacctcctc gtactccgcc
     1081 ttcgtggcga gctcctcgat ggggtatccg cggtagatga gccggttgtc ttcgaccttg
     1141 cagatggcgg tcgaggtggc caccacgcct tcgagaccgc gcggagacgc ctggggcttc
     1201 ggttctgtag tcatgtcccg agtgtggcat agcggtgtcg gtcgcctgct agtgcccttc
     1261 cgaccctctt gcgggggccg accggcgagg gtagagtccg gcgagcaggg tcatccacca
     1321 ctaaaccctg tggggaggac gaaacgcacg taccgtttcc gcgttatcag cgtggggacg
     1381 gcgcacttta gctagtccct catcccaccc accagcggag ggcggcggcc gtagggccga
     1441 cgccctttcg ctatcttccg cgttagtgcc acagatgaag gccactgtgc gtcaggagcg
     1501 gcgccctgcc gtcccccgtc agcgctaccg ggcgctcgcg cggcacacag gcgtacgagc
     1561 gcggcttcac cgcccgctcc acctcgtgga cggggccgtc ctcgtcctgg ccggggcgtt
     1621 cctcatcttc ttcgccggac cctcgctcgg cggcgccctc ggcgacggcg cggaggacat
     1681 ccgcgcacag gtcagcgcga tgttcccgac gctccagggc acagggtcga tccagctgcc
     1741 gagcggcggc ggcaccgtcg cggcggaccc ggtcgcgaac ggccttcccg acttcacgcg
     1801 cgagcctgcg ctcacggtga acggtcgcgt cccgaccttc gcggtcacgc ccggccgcag
     1861 cgtgcaggtc gcgctgaacg gggcgctcgt cgcgacggtg acgccggacg cgagcggcgc
     1921 gttcaccact gcgctcacgc tgcgcgaggg gccgaacgcc atcacgctca cgctcctggg
     1981 cacggccgac atcgtcgccc actcctcgta taccgtcgtg ctcgatcgcc agccgccgac
     2041 gctcgccgtg tccaagccgg ccaacggcga gaccgtcgaa ggcccgaacg tcgccgtcca
     2101 gggaaaggtc gaagccggcg cgacggtcat cgtgaacgac cgcatcgtcc tcacggcgcc
     2161 ggacggcagc ttcagcgagt cgttcggcgc atcgcccggg ccgctgccca tcaccgtcac
     2221 ggcgcgtgat cgcgcgggca acgagacgac ggtgaagacc gccatcacgg tcgccgcgcc
     2281 cgtcacgacg gcgccgctcg ccgtgaacgt gacgctcgac aagatcaagg tcacgccggg
     2341 cgcatgggtg atcgcggcga tccgggtgac ggcgaacggc acgccgcgct ccggcgagca
     2401 ggtcacgctc tccgtgggcg tcatcacgat cggctcggcg acgaccgacc cgagcggtgt
     2461 cgcgcgcatt ggattcgcgg cgccgcccaa cgaaggcgac gcggccgtcg tcgtgctcgc
     2521 gaacggggcg accggaaggg ccacgctcac cgtcgcgaag tgacgtccgg cgcgatactg
     2581 cgccgatgac ctccgccgac ctcgtcctct tctcgatcct cggcggcacc gcgctgctcc
     2641 tctacggcgt acggctcgtc ggcgagggcc tgcagcgtgc cgccgggacg cgcctgcggc
     2701 atctgctctc gacgctcacg ggaaaccgtc tgaaggcgct gctcgtcggg gcgggcgtga
     2761 cggcggtcct gcagtcctcg agcgccacga cggtgatgct cgtgggcttc gcgtccgcgg
     2821 ggctgctgtc gctgcgccag acgatcggcg tcatcctcgg cgcagacatc ggcacgacgg
     2881 tcaccgtgca gctcctcgcc ttcgacctgc tcgcggtgtc gccgctcatc gtcttcatcg
     2941 gttggatgct ctgggcgacg ggccgcggga cgccgaagta cgtggggcag gccatcctcg
     3001 gcttcggatt cctgttcctc gggatgaagc tcgtcgcaga cggcaccgcc ccgctggcga
     3061 ggagcgcgct cttcgccgac gtgctcgccg ctctggcgga ccagccgctc atcctcctca
     3121 tcatctcggc ggccttctcg ggcctggtgc gctcgagcgc cgccacgatc ggcttcgcgc
     3181 tgtccctcgc cgccgcgggg ctcctcccgc tcgccggcgc gatcccgatc atcttcggcg
     3241 cgaacatcgg gaccgccgtc accgcgatcc tcgcggccat cgggcagaac gccgaggccc
     3301 gccgcgtcgc cgcggcgcat gccgtcttca aggtcgtcgg cgtcgcgctc ttcttcccgt
     3361 tcgtcacgcc gttcgcggag ctcgtctccc gcaccgcgcc ggacgtcccg cggcagatcg
     3421 ccaacgcgca caccatcttc aacgtcgccg tggcggcgat cttcctgccg gccagctcgt
     3481 gggcggcggg cttcttcacg cgcacgatcg cggatcgcgc gagcgcaccc accggcgcga
     3541 tctacctgaa tgcgcagacc ctggacacgc cggcggtcgc gctcggacag gcgctccggg
     3601 agacgctccg gatgggcgat gtcgtcgtgc gctcgctgcg cgactcgatc gtcgtgctcg
     3661 agcgcaacga tgacgcgctc atgcggcagg tgatcgcccg cgatgacgtg atcgacgggc
     3721 tcgaggagga catcaagcag ttcctcatca agctctcggc gcacgcgctc accgaggagc
     3781 aggccgagcg ggagaccgcg ctcatcttcg tcatcgcgaa cctcgaggag atcggggacg
     3841 tgatcgagaa gaacctcatg gagctcgccg agaagaagat ccgcggcggc cacgtcttca
     3901 gccagcaggg ctggcacgag atcgcgggcc tgcacgcgaa ggtcgtcgag aacgtcgagc
     3961 tcgcgctctc tgccctggcg gcgaacgacg cggacgtcgc cgaaaaggtc atccggcaca
     4021 agacgcgcgt gaacctgatg gagcggcagt tccgtcagac gcacatccag cggctgcacg
     4081 aggggctgcg cgaatcgatc gacacctcga gcctgcacct cgacctgctc gccaacctca
     4141 agcgcgcgaa ttcgctcgcc gcgggtatcg cctatgccgt gctcggccgt catgcggtca
     4201 ccgacgacga tgcctagggc gtacgtcggc acgtcggggt ggtcctacac gacctggcgg
     4261 ccgaagttct accccgaggg caccaagtcc aaggacttcc tcgcgtacta cgcgacgaag
     4321 ttccggacga tcgagatcaa ctacacgttc aaccacctgc ccaccgagaa gaacgtcgcc
     4381 gagtggaccg ccgcgaccgg cgatgacttc gtgttcgggc tgaaggcgtc gcagcagatc
     4441 acacatcgcg cgcagctgcg cgagccggcc gagacgctgc cggtcttctt cgagcgcgca
     4501 cggctgctcg gcgcgcgctt ggggccggtg ctcttccaga cgccgccctg ggtgaagcgc
     4561 gacgacgacc gcctcgcgat gttcctcgcc gcgctcccgc gggacgtgcg ctgcgtgctc
     4621 gaggtgcgtg acgcctcgtg gtacgcggat ccggtgtacg agctgctccg cacggccaac
     4681 gtcgcgctcg tgcatgcgga gggggagggg catccctcgc cggtcgagac gctcggcgcg
     4741 accgccacct tcgcgtacgc gcgcctgcgt gcgcgcgagg gctatcccga cgcggccgtc
     4801 gacgcgtggg cggagcgcct gcgcgcgatc atcgcggcgg gcaaggacgt ctacgcgtac
     4861 ttccgtcacg acgacgacgg atcgaacgcg ctctcggcca tgcgccttcg cgacgccctc
     4921 gcgtgatcct cggcgagagc gtctcggcga tcatcatcgc gtcgttcgcg gcgttcatgg
     4981 ccgtcgcgca ggtcagccgc gcgcgcgcga tcgcgcgccg tggaccgtcg ctgcgccgcg
     5041 ccgggggcat cgccgatctc gcgacctact tcttctggct tccgtacctc gtcgtctggc
     5101 tgcggccggg tccgacgctc gtgctgcatg aggcgctcgg ctggctcggc ctcgcgctcg
     5161 cggtgggcgg tgtcgtcttc gcggtggcgg ccgtggcgac gctcggacgc cactacgacc
     5221 tcaagctcga gctccatgcc ggccacgaga tcgtccgacg cggtccgtac ggggtcgtgc
     5281 gccatccgat ctacacgggt ctcgcgatcc actcgctcgg cgcgtgcctc gcgacgggga
     5341 acctgatctt catcgccggc acgctggcga tcacgttccc ggtcttcgtc gcgcgtgcgc
     5401 gcgccgagga gcgtctgctg cgtgaggagt tcggcgcgga gtacgaccgc tacgcccgcg
     5461 aggtgccgat gctcgtgccc ggtcttcgtt aagcgggctt cgggaagagc cgcgcgccca
     5521 cggcggacag cgcgatcgtc agggccgccg cggccagtgc cgcgctcgac tggccggcac
     5581 tgacggcgcc cacggcgacg ccgaggccga gcacgacgaa gctgaactcc ccgatctgcg
     5641 cgagccccac gcccagctgc cagtgacgtg cgggcacccg gaagacggcc gcaagcaccg
     5701 tgatgaccac ggccttcagc gcgaccaaag ccacgaacgc gaccagccac cccggctcgc
     5761 gggccaccgc cgcggggtcg accagcgtcc cgatggccac gaagaacagc accgcgaaga
     5821 ggtcgcggaa cggcaggacc tcgcgacgcg cctcgcgtgc gatcgggcgc accgagaaga
     5881 gcgtccccgc gacgaacgcc gcgagggcga cgggcacgcc gaacacgacc gcgccgagtc
     5941 cggcgatcgc gagcgcggcg gagaccgcgg cgatgaggaa ggagtcgggc tggtccttga
     6001 ccgcgccgag gagccgcggc acgacccagc cctgcaccag cgcggcgagc atcacgaagg
     6061 cgagcgtccg cgcgagcgcg acggcgggcg actcgtcgcc gggagcgagc agcaccgcga
     6121 ggatcgtcgc ggccacgagc gtgaccgcgt cctgcaggat cgcccatacg acgagggcgc
     6181 gttcggtgtc gcgatcggtg gtccgcttcc ggctccggga gatgttcacc acgacgaccg
     6241 agctcgagag cgccgcgacg agacccagcg tcaccgcgcc gagcacgccg gtgccgagtg
     6301 cgacggcgac ggccgcgcct atcgccgtca cgatcgcgat ctgcagcggc accgcgacga
     6361 ggatgccgcg cggctcgcgc gccagcgaac gcaggtcgag ctcgatgccc acctcgaaga
     6421 gcagcaggac gacccccacg tcggcgagca gctggatctg acggcggtcc gcgacgtagc
     6481 cgggggtgaa cggcccgacg gcgaggccga cgacgagata gcccacgacc gcgggaagcc
     6541 ccacgcgccg ggcgagcacg cccgcgacgc cggcgacgag cagcacgagc ccgatctcga
     6601 ggaccgcgac ggcgctctcc acgggcgaag gctacccgcc tactggagga cggccgcggt
     6661 cgtcgtgagg tccgtctctg agcccatcgc gacgacgaga tcgccgtcgt cgatgcgcag
     6721 cgtctcgtcc gggttgacgt ggagcgtgcc atcgcggcga cggaccgcga gcagcctcgc
     6781 atgaccggcg tcgagcagcc cggcctcgcg cagcgtgcgc ccgcgcgccg cgcacgtggc
     6841 gccgaccagg atctcctcga tgccgatggc gctcccgccg tgctgcatcg tgtcgatcac
     6901 gtcgacgacc gcgggccgga tcgcgagctg cgccatgagc cggccggcca tgcggtacgg
     6961 cgacacgacg cggttcgccc cggcgagctc gcgccggcgc ggcgactccg gctggcccgc
     7021 gcgtgccgtg atgtacaggc cggggttcag cgcgcgtgcc gcgagagtga tgtacacgac
     7081 gcgctcgtcc gagtcgacga ccgcgaggag ccccttggcg tgggcgatcc ccgcgctctt
     7141 cagcacgtcc tcggaggcgg cgtcgccctc cacgaagggc cggccctcgc ggcggagccg
     7201 cgcgagcggc tccgcgttca cctcgacgac gacgtaggcc acgtggtgct
//
LOCUS       DASTZR010000437         3641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1146985, whole genome shotgun sequence.
ACCESSION   DASTZR010000437 DASTZR010000000
VERSION     DASTZR010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146985"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VFV20_09725"
     CDS             <1..188
                     /locus_tag="VFV20_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288672.1"
                     /translation="KPNIGDRYDSRWGTSVIVARSDDPTTGTYLLLRILPHGYTGKAA
                     EIGTQRRDAPRELVGKI"
     gene            complement(398..1141)
                     /locus_tag="VFV20_09730"
     CDS             complement(398..1141)
                     /locus_tag="VFV20_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288673.1"
                     /translation="MSERAAVAPSERDVLVLAKTVAANEFPVERPSVIGIDRESGVLL
                     RLSPFPWKGNDTDPPIAKWGWLRVRTVAGERDPRPETVGVEGEPAMTAYVDAKEAWRL
                     RWPFVRPHLRGSVESLVELARQRVATAGFVKPLDADIIQLPLRYRFRCANDECVTTHD
                     LPVLDWELQEMARLSRERYGPQWATRFRESWGAGLFERFDVHLLLSSYAQAPTKLYVA
                     GLFTPPKQAEVEHAHAHHVEHRSHREPPA"
     gene            complement(1138..1812)
                     /locus_tag="VFV20_09735"
     CDS             complement(1138..1812)
                     /locus_tag="VFV20_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEU5288674.1"
                     /translation="MRPIHAVLFDWGDTLFESPHAPTVIRDAARARGIAMDPGDARRI
                     WTELWDAGKTAEEHAKGRDLSRDAHRRVWTALFARAEPRVPGIAQALYDNVMDPARWI
                     PYPDTEPTLRALKERKVRVGIVSNHAYDLRPFFAAKGLDALIDDYALSFEVGTPKPSP
                     EIFLFACHLLAVDPAEALMVGDDAVSDGGAAAAGLRVHILPPHELPGSARGLADVVAL
                     VDASRR"
     gene            complement(1815..2618)
                     /gene="iolB"
                     /locus_tag="VFV20_09740"
     CDS             complement(1815..2618)
                     /gene="iolB"
                     /locus_tag="VFV20_09740"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019536524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HEU5288675.1"
                     /translation="MARLTRLRIASGAAPDGAHRLLNVTRERAGWRFVGFEVVRLAAG
                     QTVERDTRDREACLVWLSGTSAVSVAGERWPRVGGRASPFDGPPDAVYAPPAARVRIR
                     ALDTCEIALGWAPAERGAAARCLDGARVELRGEGVVQRTIHQILMEDREAERLLITEV
                     LTPAGHWSSYPPHKHDTDDPPNETYLEETYYFRMRDPRGFALQRVYTADRSLDESVAV
                     RDGDLVLVPRGYHTVSAAPGYETYYLNVMAGPRREWKITFDPDHERMRW"
     gene            complement(2606..>3641)
                     /locus_tag="VFV20_09745"
     CDS             complement(2606..>3641)
                     /locus_tag="VFV20_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU5288676.1"
                     /translation="IGVTGTSAANRVAAEADLVIGIGTRWSDFTTGSRALFARADVRF
                     ANVNVASVDASKMGGLAVVADALAALDLLSRVDYRVDDAYRAEIARRKAEWDRAVERM
                     VATRHTPLPAQHEVIGAVNEAMGEHDVLVCAAGSLPGELHKLWRARDPKQYHVEYGYS
                     CMGYEIAGGIGVKLAAPEREVFVMVGDGSYLMMPGEIVTAVQEGLKIVIVLVDNQGHS
                     SIGALSRSLGLEGFGTQYRYRKNGSLGLDSDRNTLNALPVDLAANAASLGARVLRAGT
                     IEELRAALVQARAATETTVVYVQVDRYAGVDAGGAWWDVATAEVAGSDAAREANERRT
                     TERRRQRWHV"
BASE COUNT          500 a         1354 c         1258 g          529 t
ORIGIN      
        1 cgaagccgaa catcggcgat cgctacgact cgcggtgggg gacgtcggtc atcgtcgcgc
       61 gcagcgacga tcccacgacc ggcacgtacc tcctgctgcg catcctcccg cacggctaca
      121 cgggcaaggc agccgagatc ggcacccagc gccgagacgc accgcgggag ctcgtcggca
      181 agatctagtc ttcgctctcc aatgcgactc gcgtcctgcc cttgtctgac ctcgccgcgc
      241 gtcgaccgaa accctctcca cgccgcgcac cccatccctg gtcgggatgg ggacccccgc
      301 gtcgcggggt ccctcggtcg acgcgctgac atcgtcgtct cggattccgt tcggtcgcga
      361 ctgcccgcgt tcctctgtct cgtgacgcgc gcccggctta tgcgggcggc tcgcgatggc
      421 tccggtgctc cacgtggtgc gcgtgcgcgt gctcgacctc tgcctgcttc ggtggcgtga
      481 acaggccggc gacgtagagc ttcgtcggcg cctgcgcgta ggacgagagc agcagatgga
      541 cgtcgaagcg ctcgaacagc ccggcgcccc agctctcgcg gaagcgcgtg gcccactgcg
      601 gtccgtagcg ctcgcgcgag agccgcgcca tctcctggag ctcccagtcg aggaccggca
      661 ggtcgtgcgt cgtcacgcac tcgtcgttcg cgcaccggaa gcggtaacgc agcggcagct
      721 ggatgatgtc ggcgtccagg ggcttgacga acccggcggt cgcgacgcgc tgccgcgcga
      781 gctccacaag ggactcgacg gagccgcgga ggtgcgggcg gacgaacggc cagcgcagcc
      841 gccacgcctc cttcgcgtcc acgtaggcgg tcatcgccgg ctcgccctcg acgccgacgg
      901 tctcggggcg cggatctcgt tcccccgcca cggtacgcac gcgcagccag ccccactttg
      961 cgatcggcgg gtcggtgtcg ttgcccttcc aggggaacgg cgagagtcgc agcaggactc
     1021 cgctctcgcg gtcgatgccg atcacggacg gacgctccac cggaaactcg ttcgccgcga
     1081 ccgtcttcgc gaggaccagc acgtcgcgct ccgacggcgc gacggccgcg cgctcgctca
     1141 cctgcgcgac gcgtccacga gcgcgacgac gtccgcgagg ccgcgtgcgc tccccggcag
     1201 ctcgtgtggc ggcaggatgt gcacgcgcag gcccgccgca gccgcgccgc cgtcgctgac
     1261 cgcatcgtcg ccgaccatga gcgcttccgc aggatcgacg gcgagcagat ggcaggcgaa
     1321 caggaagatc tccggcgatg gcttcggggt gcccacctcg aacgagagcg catagtcgtc
     1381 gatgagcgcg tcgagaccct tggccgcgaa gaacggccgg agatcgtacg cgtggttgct
     1441 cacgatcccg acgcggacct tccgctcctt caacgcgcgg agcgtgggct cggtgtccgg
     1501 atacgggatc cagcgcgccg ggtccatcac gttgtcgtag agggcctggg cgatcccggg
     1561 cacgcgcggc tccgcgcgcg cgaagagcgc ggtccacacg cgccgatgcg catcgcgcga
     1621 caggtcgcgg cccttcgcgt gctcctcggc ggtcttcccg gcgtcccaga gctcggtcca
     1681 gatgcggcgt gcatcgcccg ggtccatcgc gatgccgcgc gcccgcgcgg cgtcccggat
     1741 gaccgtcggc gcgtgcgggc tctcgaaaag cgtgtcgccc cagtcgaaca ggaccgcgtg
     1801 gatcggccgc atgctcacca gcgcatgcgc tcgtggtccg gatcgaaggt gatcttccac
     1861 tcgcgacgcg gaccggccat cacgttgagg taatacgtct cgtagccggg tgccgccgac
     1921 accgtgtggt agccgcgggg cacgagcacg aggtcgccgt cacggacagc cacgctctcg
     1981 tccagcgagc ggtcggcggt gtacacgcgt tgcagcgcga agccgcgcgg atcgcgcatc
     2041 cggaagtaat acgtctcctc gaggtacgtc tcgttcgggg gatcgtcggt gtcgtgcttg
     2101 tgcggcggat agctcgacca gtggcccgcc ggcgtgagca cctccgtgat caggaggcgc
     2161 tccgcctcgc ggtcctccat gaggatctgg tggatcgtcc gctgcacgac cccctcgcca
     2221 cggagctcga cgcgggcgcc gtcgaggcag cgcgccgcgg ccccccgttc cgcgggcgcc
     2281 cagccgagcg cgatctcgca cgtgtcgagc gcccggatgc gcacgcgcgc ggcgggaggc
     2341 gcgtacacgg cgtccggcgg tccgtcgaag ggcgatgcgc gcccgccgac gcgcggccat
     2401 cgctctcccg cgaccgagac ggcgctcgtc cccgatagcc acaccaggca cgcctcgcga
     2461 tcccgcgtgt cgcgctcgac cgtctggccg gccgcgaggc ggacgacctc gaagccgacg
     2521 aagcgccagc cggcgcgttc acgcgtgacg ttcagcagcc gatgggcgcc gtccggcgcc
     2581 gcgccgctcg cgatgcgcag gcgggtcaga cgtgccaccg ctggcgtcgc cgctcggtcg
     2641 tgcggcgctc gttcgcctcg cgcgcggcgt ccgatccggc cacctcggcc gtcgcgacgt
     2701 cccaccatgc cccgcccgcg tcgacgcccg cgtagcggtc gacctgcacg tacacgaccg
     2761 tcgtctccgt cgcggccctc gcctgcacga gcgccgcacg cagctcctcg atcgtccccg
     2821 cgcgcagcac gcgcgcgccg aggctcgcgg cgttggcggc gaggtccacg gggagcgcgt
     2881 tcagcgtgtt gcgatcgctg tcgaggccga gcgacccgtt cttgcggtag cggtactgcg
     2941 tgccgaagcc ctcgagcccg agcgaccgcg agagggcacc gatggagctg tggccctggt
     3001 tgtcgaccag cacgatgacg atcttgaggc cctcctggac cgcggtcacg atctcgccgg
     3061 gcatcatgag gtagctgccg tcgccgacca tgacgaagac ctcacgctcg ggcgcggcga
     3121 gcttgacgcc gatgcctccg gcgatctcgt agcccatgca cgagtacccg tattcgacgt
     3181 ggtactgctt ggggtcacgc gcccgccaca gcttgtgcag ctcgccgggc agcgagccgg
     3241 ccgcgcacac cagcacgtcg tgctcgccca tcgcctcgtt caccgcaccg atgacctcat
     3301 gctgcgcggg gagcggcgtg tgccgcgtcg cgaccatccg ctccacggca cgatcccatt
     3361 cggccttgcg cctcgcgatc tcggcgcggt acgcgtcgtc gacgcgatag tcgacgcgcg
     3421 acagcaggtc gagcgcggcg agcgcgtctg cgacgaccgc gagccctccc atcttcgacg
     3481 cgtcgacgga cgccacgttc acgttcgcga agcgcacgtc cgcacgagcg aacagcgccc
     3541 gcgacccggt ggtgaagtcg ctccagcggg tcccgatgcc gatcaccagg tccgcctcgg
     3601 cggcgacgcg gttggcggcg gacgtgccgg tcacgccgat c
//
LOCUS       DASTZR010000438         4222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_624814, whole genome shotgun sequence.
ACCESSION   DASTZR010000438 DASTZR010000000
VERSION     DASTZR010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624814"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VFV20_09750"
     CDS             <1..490
                     /locus_tag="VFV20_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014688398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5288677.1"
                     /translation="RPGRGASRLGGDDAWRLAARRLVSRVGTVFQDPEHQFVTRRVDE
                     ELTIGPIRAGRTASDARVIARDLLERLRLAHLAGANPFTLSGGEQRRLSVATALATAP
                     PLLVLDEPTFGQDRRTYGELVGLMARHRDGGGALLFASHDGMLVEALADRVVSLRAGR
                     AA"
     gene            487..1242
                     /locus_tag="VFV20_09755"
     CDS             487..1242
                     /locus_tag="VFV20_09755"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEU5288678.1"
                     /translation="MRLLAPLVPDPRAPLARAHPISKIVAGLVVMLGLFITIDIVTST
                     LVLIALSVAAALSGLPVRTLALRAVPLLISACGIGVFNAAVGSTGVAGGVAIALRLIG
                     ISLAGILAVATIDPTDLADALVQHVHAPPRFVVGALAAFRLFPLFSREWDTIGLARRA
                     RGIEADRGFTARLSAFPGRTLGLLVAAIRRATRLALAMDARGFGSRTCRTLARPRALT
                     RRDGVLVALALAVVVLATGVSMSLGTWRPLLVL"
     gene            complement(1276..2274)
                     /locus_tag="VFV20_09760"
     CDS             complement(1276..2274)
                     /locus_tag="VFV20_09760"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEU5288679.1"
                     /translation="MRRILVVGAGFFGALVARRLREVGLAPLVATRRGADVRLDAEDE
                     VSIDAVLRPGDVIVDTAGPFALRSTRLVRAAIDRGCDVIDLSESLAWAEAIVALGSRA
                     AAAGVRLYPACSAVAAVTGACVATSGIAGPRSVDQFLAPASAETASPATIASFVRSLG
                     VPIRTLRDGALVTVRGYGETRAFPGSRRRGGLVESASAVLLPRSWPSLRRAEFWVDPN
                     TPLGRTALSAAAGLTPLAALARQVASTLPARRIGRHDGIFAVAVADGSRCVSFALTAP
                     RRSYLIAVEPAVIAAEALARGPRGGAGIVAPHAQVDAELLFARLRSLAIDVRGATL"
     gene            complement(2271..2852)
                     /locus_tag="VFV20_09765"
     CDS             complement(2271..2852)
                     /locus_tag="VFV20_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288680.1"
                     /translation="MAPLACAAANLVAALALAFVLAPATPLVADAAERERYVREHLVA
                     WRVGWATWMLAGASLIWMYAWWRRRVGAPRWVLAIAIAGIAADWTAELMLIVGAPGRY
                     ADVAPIAFFLTGAVANGLYTLAGIQLTLATPLGLGARVYAALMWSAGLSLSAGAILAI
                     PLVTAIASAELFILFVPWCLWLWRHFRPRGLSA"
     gene            2882..3451
                     /locus_tag="VFV20_09770"
     CDS             2882..3451
                     /locus_tag="VFV20_09770"
                     /inference="COORDINATES: protein motif:HMM:NF021996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HEU5288681.1"
                     /translation="MLRRRMVRAAAIVATLALAATAWYLGSPLFIRTTLDEPLPGGAV
                     AGTSAPAPSPIGTAGASAAASAAATRTTQTTVQFRGQLQYVDALHNGTGTVLGMVIAS
                     CCPVSDQYLLRFEDVAITNAPDVHVYLSPERGGRWSEATSKYLGPLKATNGSFNYAIE
                     PSDLGIYRSVVVWCRAFAVLVTWADLDRA"
     gene            complement(3461..4132)
                     /locus_tag="VFV20_09775"
     CDS             complement(3461..4132)
                     /locus_tag="VFV20_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5288682.1"
                     /translation="MTATILVVDDEPAMVGMVGALLGEEGYQIVTAYDGEVALRRHSD
                     ERPDLVILDRRLPRMSGDDVCRSIRAVSSTPILMLTGEKGSEERARLLDLGADDYLEK
                     PFSKKELSARVRALLRRAAAPVRADVAAHVGRLSVDARTHRATIDGAALDLTPTEFRL
                     LAALAARPGEVVDRKALLRAGWPEERDPDPEWLKAHLARLRSKLQEEGGPDLANVRGV
                     GYRLG"
BASE COUNT          558 a         1583 c         1544 g          537 t
ORIGIN      
        1 ccgtccgggg cgcggcgcga gccggctcgg cggcgacgac gcgtggcgcc tcgcggcgcg
       61 gcgcctcgtc tcccgcgtcg gcacggtctt ccaggatccc gagcaccagt tcgtcacccg
      121 tcgtgtcgac gaggagctca cgatcggacc gatccgcgcg ggccggaccg cgagcgacgc
      181 gcgcgtcatc gcgcgcgatc tcctggagcg gctgcgcctc gcgcatctcg ccggggcgaa
      241 cccattcacg ctctcgggcg gagagcagcg gcgtctctcg gtggcgaccg cgctcgccac
      301 cgcgccgccg ctcctcgtgc tcgacgaacc cacgttcggt caggatcgcc gcacctacgg
      361 tgagctggtc ggcctcatgg cgcggcaccg cgacggcggc ggcgcgctcc tgttcgcgtc
      421 gcacgacggg atgctcgtcg aggcgctcgc cgatcgcgtg gtgtcgcttc gcgcggggcg
      481 tgcggcatga ggctgctcgc gccgctcgtc cccgatccgc gcgctccgct ggcgcgggcg
      541 catcccatct cgaagatcgt ggccggcctc gtcgtcatgc tcggcctctt catcacgatc
      601 gacatcgtga cctccacgct cgtgctgatc gcgctgtcgg tcgccgccgc gctgtcgggc
      661 ctgccggtgc ggacgctcgc gctgcgcgcc gtgccgctgc tcatctccgc gtgcggcatc
      721 ggcgtcttca acgcggccgt cgggtccacc ggcgtggcgg gcggcgtcgc catcgccctg
      781 cggctcatcg ggatctccct cgcggggatc ctcgcggtcg cgacgatcga cccgacggac
      841 cttgccgacg ccctcgtaca gcacgtccac gcgccacccc ggttcgtcgt cggcgcgctc
      901 gccgcgttcc gactgttccc gctcttctcg cgcgagtggg acaccatcgg cctcgcgcgc
      961 cgcgcacgcg gcatcgaagc ggatcgtggc ttcacggcgc ggctctccgc gtttccgggc
     1021 cggacgctcg ggctgctcgt cgcggcgatc cgcagggcga cgcgcctcgc gctcgcgatg
     1081 gacgcgcggg gcttcggttc ccgcacctgc cgcacgctgg cgcgaccgcg cgcgctgacg
     1141 cggcgcgacg gggtgctggt cgccctcgcg ctcgccgtcg tcgtacttgc gacgggcgtg
     1201 agcatgagcc tgggcacctg gcgtccgctc ctcgtcctct aggacccggc ggtccgcgcc
     1261 cgaggctcga gtcggctaca gcgtggcgcc gcgcacgtcg atcgcgagcg agcgcaggcg
     1321 cgcgaacagc agctcggcgt cgacctgcgc gtgcggcgcc acgatccccg cgcctcccct
     1381 cgggccgcgc gcgagcgcct ccgcggcgat caccgcgggc tccaccgcga tgaggtagct
     1441 gcggcgcggc gcggtgagcg cgaaggacac gcaccgcgat ccgtccgcga ccgcgacggc
     1501 gaagatgccg tcgtgccggc cgatgcgccg ggcgggcagg gtcgacgcga cctgtcgcgc
     1561 gagggcggcg agcggtgtga gcccggccgc ggcggagagg gcagtgcgtc cgaggggcgt
     1621 gttcgggtcc acccagaact ccgcacgccg gagcgacggc cacgaccggg gcaggagcac
     1681 ggctgacgcg ctctccacga ggccgccacg ccgccgcgag cccgggaacg cgcgcgtctc
     1741 gccgtatccg cggacggtga cgagcgcgcc gtcccgcagc gtgcggatcg gcacgccgag
     1801 cgagcgcacg aaggacgcga tcgtggcggg gctcgcggtc tcggccgagg ccggcgcgag
     1861 gaactggtcg acgctccgcg gtccggcgat accgctcgtc gcgacgcacg caccggtcac
     1921 ggcggcgacc gcgctgcacg ccggatacag gcggaccccg gccgcggccg cgcgcgaccc
     1981 gagcgccacg atcgcctcgg cccaggcgag cgactcggaa aggtcgatca cgtcacaccc
     2041 gcgatcgatc gccgcgcgca cgagccgcgt gctgcgcagt gcgaacggcc cggccgtgtc
     2101 cacgatcacg tcgcccggcc gcagcaccgc gtcgatcgag acctcgtcct ccgcgtcgag
     2161 acgcacgtcg gcaccgcgcc gcgtcgcgac gagcggagcg agcccgacct cacgcagccg
     2221 ccgcgcgacg agcgcgccga agaatcccgc gccgacgacg aggatgcgcc tcacgcggag
     2281 aggccgcgcg gcctgaagtg tcgccacagc cacaggcacc acggcacgaa caagatgaag
     2341 agctcggcgc tcgcgatcgc cgtcacgagc gggatggcga ggatcgcgcc ggcgctgagc
     2401 gagagcccgg ccgaccacat gagggccgcg tacacgcgcg cgccgaggcc gagcggcgtc
     2461 gccaacgtga gctggatgcc ggcgagcgtg tacaggccgt tcgcgacggc gccggtgagg
     2521 aagaacgcga tgggggctac gtcggcgtag cgacccggcg caccgacgat gagcatcagc
     2581 tcggcggtcc agtcggccgc gataccggcg atcgcgatcg ccagcaccca gcggggcgcg
     2641 cccacgcggc ggcgccacca cgcgtacatc cagatgagcg acgcgccggc gagcatccag
     2701 gtcgcccacc cgacgcgcca cgcgacgagg tgctcgcgca cgtatcgctc gcgctcggcg
     2761 gcgtcggcga cgagcggcgt cgcgggtgcg aggacgaacg cgagcgccag cgcggcgacg
     2821 agattcgcgg ccgcgcacgc gaggggcgcc acggcggcga ctctaccccg atactgcgtg
     2881 gatgctgcgc aggcggatgg tgcgcgccgc ggcgatcgtc gccaccctcg ccctcgcggc
     2941 gaccgcgtgg tacctcgggt cgccgctctt catccgcacc acgctggacg agccgctgcc
     3001 gggcggggcg gtggccggta cgagcgcgcc ggcgccttcg ccgatcggga ccgccggcgc
     3061 gtcagccgcg gcctcggcgg cggcgacgcg cacgacgcag accacggtcc agttccgcgg
     3121 ccagctccag tacgtcgacg cgctgcacaa cggcacgggc acggtgctcg gcatggtcat
     3181 cgcgagctgc tgcccggtga gcgaccagta cctgctccgg ttcgaggacg tggcgatcac
     3241 gaatgccccc gacgtgcacg tgtacctgtc accggagcgc ggcggcagat ggagcgaggc
     3301 gacgagcaag tacctcggac cgctgaaggc gacgaatgga tcgttcaact acgcgatcga
     3361 gccgtcggac ctcgggatct accgcagcgt cgtcgtgtgg tgtcgcgcgt tcgcggtgct
     3421 cgtgacctgg gccgacctcg atcgagccta gtgtcgtgcg ctacccgagc cggtatccga
     3481 cgccgcggac gttcgcgagg tcgggcccgc cctcttcctg cagcttcgag cgcaggcgcg
     3541 cgaggtgcgc cttcagccat tccggatcgg gatcccgctc ctccggccat ccggcgcgca
     3601 gcagggcctt ccggtcgacg acctcacccg gacgcgcggc gagcgcggcg aggagccgga
     3661 actcggtcgg cgtgagatcg agggccgcgc catcgatcgt ggcgcgatgg gtacgcgcgt
     3721 cgacggagag gcgaccgacg tgggcggcaa cgtcggcgcg caccggcgcg gccgcgcgtc
     3781 gcagcagcgc gcgcacgcgt gccgagagct ccttcttgct gaacggcttc tcgaggtaat
     3841 cgtcggcgcc gaggtcgagc agccgcgcgc gttcctcgct gcccttctcg ccggtgagca
     3901 tgaggatcgg ggtgctcgag accgcccgga tgctcctgca cacgtcgtcg ccggacatgc
     3961 gcgggagcct gcggtcgagg atgacgagat cgggacgctc gtcggagtga cgccgcagcg
     4021 ccacctcacc gtcgtacgcg gtgacgatct gatagccctc ctcgccgagg agcgcaccga
     4081 ccatcccgac catcgcgggc tcgtcgtcga cgacgaggat cgtcgcggtc acgcgtcacc
     4141 cttggcgagc ggcacgctga acgcgaacga ggcgccgccc gacggcacct cttcgtacca
     4201 gaggtcgccg cccagcgcct cg
//
LOCUS       DASTZR010000439         3769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_755516, whole genome shotgun sequence.
ACCESSION   DASTZR010000439 DASTZR010000000
VERSION     DASTZR010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755516"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFV20_09780"
     CDS             <1..345
                     /locus_tag="VFV20_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288683.1"
                     /translation="RALGRAIADARDARRRRRWAAAARRRGPLLPRWRDTERAFLSLV
                     VAEMHEMPGGGDGMPPRGVKSPKRKRQYEKIKKSVMKRGRSAKTAKRIAAATTNKTRR
                     RKGETKTARKKS"
     gene            complement(368..574)
                     /locus_tag="VFV20_09785"
     CDS             complement(368..574)
                     /locus_tag="VFV20_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288684.1"
                     /translation="MTTLMREPGTYRTKSGALVHSRAIADGRIVLEREDGRRMGGDED
                     LVKLSDDPDWPDLHHRDTDLQLFD"
     gene            953..1585
                     /locus_tag="VFV20_09790"
     CDS             953..1585
                     /locus_tag="VFV20_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983848.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEU5288685.1"
                     /translation="MAKETGRAYPAPDNARSLRSVATAARGCRACDLWRTGTQTVFGD
                     GPRNAGVMLVGEQPGDVEDRRGEPFVGPAGRVLDEALAAAGIDRARVYVTNAVKHFKW
                     RPRGKRRLHEKPNGTEVAACRHWLELELRLVRPDLVVALGATAAQALLGPAFRVTKER
                     GKLRSSPLAERVVATVHPSSILRAPDDRRDLELKAFVKDLVAAKKLLRES"
     gene            complement(1589..2452)
                     /locus_tag="VFV20_09795"
     CDS             complement(1589..2452)
                     /locus_tag="VFV20_09795"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEU5288686.1"
                     /translation="MRALLIVNPAAGRRHAAHGDLEQTIAVLRTARITVDRAETGPDG
                     PTSADLARRAVAEGYDVCVVAGGDGTVAPAAAALLDTRVILGILPFGSYMNIARGLGL
                     PLRPIDAAAVIARGDVHDVDVGEVHGRPFFETAGIGLDAELFVAARDAERRSWLRLAR
                     RVVRWLTHGTHEVVIDVDGARHTHRVMQILVLNSPYYAWALPLLPDASMEDGLLDVAV
                     FPRMGRLALLRSLVAVMRLRPLPVRPVRYQGARIEVSAAEALTVHADGQLAGTLPAEL
                     RCRRGALRVYA"
     gene            2603..3376
                     /locus_tag="VFV20_09800"
     CDS             2603..3376
                     /locus_tag="VFV20_09800"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU5288687.1"
                     /translation="MPRATLRARLWRRIATPLLLAGMAAVMWVLVGATQSGALRTLDS
                     VRPQATPTATPPLAALVYASVAASVVQVLATGPGNEPSSGAGVIVDEQADILTSLHIV
                     SGAKTVVVRFSDGTEAPAEITASLVDKDIAVLRPASAPAKVQPAILGDPSRLVVGAPA
                     FAVGHPFGLTASLSAGVVSGLDRSLNVPGHEKPLTGLIQFDAAVNPGNSGGPLVDSHG
                     EVVGIVTGLVNPSGQKVNSGVGFAVTIDQAAAALGIPPD"
     gene            3425..>3769
                     /locus_tag="VFV20_09805"
     CDS             3425..>3769
                     /locus_tag="VFV20_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5288688.1"
                     /translation="MTIAARPGSQMERLLFEIKKVIVGQDELLERIIVALLARGHLLV
                     EGVPGLAKTLAVKTVAQAISGEFKRVQFTPDLVPADLVGTRIYNQKSGEFQTSLGPVF
                     TNLLLADEINRAP"
BASE COUNT          577 a         1339 c         1348 g          505 t
ORIGIN      
        1 agggcgctcg gtcgcgcgat cgcggacgcg cgcgacgcac ggaggcgccg gcgctgggcc
       61 gcggccgccc gcaggcgtgg cccgctgctg ccacggtggc gcgacacgga acgtgccttc
      121 ctctcgctgg tggtcgcgga aatgcacgag atgccgggcg gaggtgatgg catgccccca
      181 cgtggtgtga agagcccgaa gcgcaagcgc cagtacgaga agatcaagaa gagcgtgatg
      241 aagcgcggac gatccgccaa gacggcgaag cgcatcgccg cggcgaccac caacaagacg
      301 cgccgcagga agggcgagac gaagaccgct cggaagaaga gctgacccag ccgacgcgga
      361 cgcgggccta gtcgaagagc tgcaggtccg tgtcccggtg gtgcaggtcg ggccagtcgg
      421 gatcgtccga cagcttcacc agatcctcgt cgccgcccat gcgcctgccg tcctcgcgct
      481 cgaggacgat ccgtccgtca gcgatagccc gcgagtgaac gagcgcgccc gacttcgtgc
      541 gataggtccc aggctcgcgc atgagcgtcg tcatggccgt cctcccccag tcgcagctgt
      601 gcggcttcgc ggatcagcgt ctcgagcggc acaccgcccg tcgcgaccca gcgatggcgt
      661 tcgtccatga cgtctccgtc gcagtacgcg gtcgtgccgt ggcggatgac gatgccgccg
      721 cgtccgcggc gcggcggtgc cgcgagatgc gacggatcgt cgcaacggaa atgaatgagc
      781 atcgacaccc gcgcccgcct cccctttgca acggtcgtgt gacgacagtc atgttatgca
      841 ggggcgcacg agaagaagga acgctcgtgc gcgtgggaac ccactcgccg agcaagcgtc
      901 gccggccgcc ggggccgggt tcgcggcatg acgggtgcgc cgagtggtac cgatggccaa
      961 ggaaacggga cgcgcgtatc ccgctccgga caacgcacgc tcgctccgga gcgtggccac
     1021 cgccgcacgc ggctgtcgcg cgtgcgacct ctggcggacc ggcacgcaga cggtcttcgg
     1081 cgacggcccg cggaacgccg gcgtcatgct cgtcggcgag cagccgggcg acgtcgagga
     1141 tcggcgcggc gagccgttcg tcggtcccgc gggccgcgtg ctcgacgagg cgctcgcggc
     1201 cgcgggcatc gatcgcgcgc gcgtctacgt gaccaacgcc gtgaagcact tcaaatggag
     1261 gccgcggggg aagcgccggc tccacgagaa gccgaacggg accgaggtcg cggcctgccg
     1321 gcactggctc gagctggagc tccgcctcgt ccgcccggat ctggtcgtcg cccttggcgc
     1381 caccgccgcg caggcgctcc tcggaccggc attccgcgtc acgaaggagc gcgggaagct
     1441 gcggtcgtca ccgctcgcgg agcgggtggt cgcgacggtg cacccgtcgt cgatcctgcg
     1501 cgcgcccgac gatcggcgtg acctcgagct gaaggccttc gtgaaggacc tggtggccgc
     1561 gaagaagctg ctgcgcgaga gctgacggtc acgcgtagac gcgtagcgca ccgcgccggc
     1621 atcgcagttc ggccggaagc gtgcccgcga gctgaccgtc ggcgtgcacc gtgagcgctt
     1681 ccgcggcgct gacctcgatg cgcgcgccct gatagcgcac gggtcggacc ggaaggggac
     1741 gcagacgcat gaccgccacg agcgagcgga gcagtgcgag ccggcccatc cgcgggaaca
     1801 cggccacgtc gagcaggccg tcctccatgc tcgcgtcggg cagcagcggc agcgcccacg
     1861 cgtaatacgg actgttgagg acgaggatct gcatcacacg atgcgtgtgc cgtgcgccgt
     1921 cgacgtcgat gacgacctcg tgcgtcccgt gcgtgagcca tcgcacgacc cggcgcgcga
     1981 gacggagcca ggatcgccgt tcggcgtcgc gtgccgcgac gaagagctcc gcatcgagac
     2041 cgatccccgc ggtctcgaag aacggacggc cgtgcacctc gccgacgtcc acatcgtgca
     2101 cgtcgccgcg cgcgatcacg gccgccgcgt cgatcggccg gagcggcagg ccgaggcccc
     2161 gcgcgatgtt catgtagctc ccgaagggca ggatgccgag gatcacgcgc gtgtcgagga
     2221 gcgccgcggc cgccggagcc acggtcccgt cgccacccgc gacgacgcag acgtcgtatc
     2281 cctcggccac ggcgcgccgc gcgaggtccg cgctcgtcgg cccgtcggga ccggtctcgg
     2341 cgcggtcgac ggtgatgcgg gccgttctca ggaccgcgat cgtctgttcc agatccccgt
     2401 gcgcggcgtg gcgtcgcccg gccgcggggt tgacgatcaa gagcgcgcgc atggcgcaca
     2461 gagatgcaac cgcgaggccg gaaatcgggt actccgcccg tacactggtg ccctcctcat
     2521 gacgtcgtcc cggtccgatc ggccatacgg ctcctttccg gggtcgggca cgacgccgcc
     2581 cccgcccgag gcaccgcgac cggtgccgcg agcgacgctc cgcgcgcgcc tgtggcgccg
     2641 gatcgccacg ccgctgctcc tcgccggcat ggccgcggtg atgtgggtgc tcgttggcgc
     2701 gacgcagagc ggcgccctgc gcacgctcga ttctgtgcgg ccgcaggcga cgccgacggc
     2761 gaccccgccg ctcgccgccc tcgtctacgc gagcgtcgcc gcctcggtcg tgcaggtcct
     2821 ggcgaccgga cccggcaacg agccctcctc gggcgccggc gtgatcgtcg acgaacaggc
     2881 cgacatcctg acgagcctgc acatcgtgag cggtgcgaag accgtggtgg tccgcttctc
     2941 ggatgggacc gaggctcccg cggagatcac cgcctcgctc gtcgacaagg acatcgccgt
     3001 cctgcggccg gcgtcggcgc cggcgaaggt ccagcccgcg atcctgggcg atccgagccg
     3061 tctcgtggtc ggagcgccgg cattcgccgt cgggcacccg ttcggtctca ccgcctcgct
     3121 gtccgcgggc gtcgtctccg gcctcgaccg ttcgctgaac gtccccggcc acgagaagcc
     3181 gctgacgggc ctcatccagt tcgacgcggc ggtgaatccg gggaactcgg gcggcccgct
     3241 cgtggacagt cacggcgagg tcgtcggcat cgtcaccgga ctcgtcaacc cgtccggtca
     3301 gaaggtgaac agcggcgtcg gcttcgcggt gacgatcgat caggccgcgg cggccctggg
     3361 catcccgccg gactagcggt acgtatcggg tacaagtaac agggaatagg ggagacggga
     3421 cgacatgacg atcgcagcac ggcccggcag ccagatggag cggctcctgt tcgagatcaa
     3481 gaaggtgatc gtcggccagg acgagctgct cgaacggatc atcgtcgccc tgctcgcgcg
     3541 cggccacctg ctggtggagg gcgtgcccgg gctcgcgaag accctcgccg tgaagaccgt
     3601 cgcgcaggcg atctccggcg agttcaagcg cgtgcagttc acgcccgacc tcgtccccgc
     3661 ggacctcgtc ggcacgcgca tctacaacca gaagagcggc gagttccaga cgtcgctcgg
     3721 gcccgtgttc accaacctgc tcctcgccga cgagatcaac cgcgctccg
//
LOCUS       DASTZR010000440         2818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_468013, whole genome shotgun sequence.
ACCESSION   DASTZR010000440 DASTZR010000000
VERSION     DASTZR010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468013"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..1188
                     /locus_tag="VFV20_09810"
     CDS             142..1188
                     /locus_tag="VFV20_09810"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU5288689.1"
                     /translation="MKISPHSGEGWAGLLAADYIDPRDLGPASPLQALAIVFLQELRD
                     KGRSEQTVPRYAAYIADFVSFMERGGKTARVSDIDIRALKAYGSHLTRRQVRSGRYGG
                     RGTISAATKNLHLIALRQLLKFGVLIDLPVPGPEKVELAKAVPPSPDARHLAEERVKR
                     LLDACETATDKGIRARALLEFLLATGCRVSEAVGLDRRQLELDRLAPTPKDGIRVVDE
                     VTVFGKGSRYRRVYLSKKAREWVQRYLAVRKDKDKALFVTQKKSADGSYRMSVWTAEQ
                     IVRDAAKRAGLAEDVSPHWLRHAAITFWAKGDLSSAQRLAGHRQIATTQRYLGSSDAE
                     LKAFYKRHVEGASG"
     gene            1266..2033
                     /locus_tag="VFV20_09815"
     CDS             1266..2033
                     /locus_tag="VFV20_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019482740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5288690.1"
                     /translation="MFNAAGAYDRFMGRYSVLLAPQMAELARVAPGQHVLDVGCGPGA
                     LAGELVHRLGPGGVTAIDPSEPFIAAVRSRYPGVDARVASAESLPFADATFDAACSQL
                     VVHFMRDPVAGIREMARVTRAGGVIVACVWDHAGRRGPLAPFWDAAREIVPDVDDEGH
                     RPGVAEGDLARIFAQAGLRDIEMRTLVAPLTHPTFDAWWEPFELGVGPAGAFVASLDP
                     ERRAALRERVRARVPARDFHQDAWAWAARGVIPGGAA"
     gene            2191..2397
                     /locus_tag="VFV20_09820"
     CDS             2191..2397
                     /locus_tag="VFV20_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288691.1"
                     /translation="MAGDSPRDAAAFLEKVRHASYRVTDDDIAALRRAGLGEDEIFEL
                     AVAASVGVALARLDAAKRAMRSTA"
     gene            2394..>2818
                     /locus_tag="VFV20_09825"
     CDS             2394..>2818
                     /locus_tag="VFV20_09825"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU5288692.1"
                     /translation="MSLRLAILERGHAPAKRLLIRAAERLGGVRFDDVARTSLYRPAF
                     FGRSWLRFTRSLMRGPSEWSAGERELLAAFVSRRNECPYCVGVHTETAALGLARPVDV
                     AMLDDWRSAPLAPHLKAAFELLEKRAADPAQLTPADFERA"
BASE COUNT          393 a         1019 c          990 g          416 t
ORIGIN      
        1 ccgggcatgc gcccgaccga ggacatcact cggattcgcg atttgcgcct caggacgggc
       61 tgcctggcgg cagtgtcatg cgggggaggg atcgggccgg gccgttcaca cttgacatgc
      121 accattagat tatctacagg aatgaagatt tcaccacatt caggcgaggg ctgggccggg
      181 ctgctggccg ccgactacat cgatccccgc gatctcgggc ccgccagccc cctacaggcc
      241 ctcgcgatcg tgttcctgca ggagctccgg gacaagggcc gcagcgagca gaccgtgccg
      301 cgctacgccg cgtacatcgc cgacttcgtg tcgttcatgg agcgcggcgg caagacggcg
      361 cgggtctccg acatcgacat ccgagccctc aaggcgtacg gcagccatct gacgcgacgc
      421 caggtccgca gcggccgcta cgggggccgt gggaccatct cggccgccac gaagaacctt
      481 cacctcatcg cgctgcgcca gctgctcaag ttcggcgtcc tcatcgacct cccggtcccg
      541 ggccccgaga aggtcgagct cgcgaaggcc gtgccgccca gccccgacgc gcgccatctc
      601 gccgaggagc gcgtgaagcg tcttctggac gcgtgtgaga ccgcgaccga taaaggtatc
      661 cgtgcccgcg cgctgctcga gttcctgctc gcgaccggct gccgcgtcag cgaggccgtg
      721 gggctcgatc ggcggcagct cgagctcgac cggctcgcgc cgacgcccaa ggatggcatc
      781 cgcgtcgtcg acgaggtcac ggtgttcggc aaagggagcc gctaccgccg cgtgtacctc
      841 tcgaagaagg cccgcgagtg ggtccagcgg tatctcgcgg tgcgcaagga caaggacaag
      901 gcgctcttcg tgacgcagaa gaagagcgcc gacggctcgt accgcatgag cgtctggacc
      961 gccgagcaga tcgtccgcga cgccgcgaag cgcgccggcc tcgccgagga cgtgtcgccg
     1021 cactggctgc gccatgccgc gatcacgttc tgggcgaagg gcgacctctc gtcggcgcag
     1081 cggctcgcag gccatcggca gatcgcgacg acgcagcggt atctcggcag ctccgacgcc
     1141 gagctcaagg ccttctacaa gcgccacgtc gagggcgcat ccggatagat gcgcaagacg
     1201 cgcgaggtcg cgatcttcga ttacgctcgc gaccgtgaga acgcgcgtgc cgacgagctc
     1261 gtagggtgtt caacgcggcc ggtgcctacg accgcttcat gggccgttac tcggtgctgc
     1321 tcgcgccgca gatggccgag ctcgcgcgtg tcgcgcctgg acagcacgtc ctcgatgtcg
     1381 gctgcggtcc cggcgccctc gccggcgagc tcgtacaccg ccttggcccc ggcggcgtca
     1441 cggcgatcga cccctcagag ccgttcatcg cagcggtacg gtcccgctat ccgggcgtgg
     1501 atgcgcgcgt cgcgtccgcg gagtcgctgc cgttcgcgga cgcgaccttc gacgcggcat
     1561 gctcgcagct cgtcgtgcac ttcatgcggg atcccgtcgc gggcatccgc gagatggcgc
     1621 gggtcacgcg cgccggcggc gtgatcgtcg cgtgcgtgtg ggatcacgcg ggccgccgcg
     1681 gtccgctcgc tccgttctgg gacgcggcgc gcgagatcgt gcccgacgtc gacgacgaag
     1741 ggcaccgtcc aggcgtcgct gagggagacc tcgcgcggat cttcgcgcag gcgggcctac
     1801 gcgacatcga gatgcggacg ctcgtcgccc cgctcaccca cccgaccttc gacgcgtggt
     1861 gggagccctt cgagctcggc gtcggacccg cgggcgcgtt cgtcgcgtcg ctcgatcccg
     1921 agcggcgtgc cgccctgcgc gagcgcgtcc gcgcgcgcgt acccgcccgc gacttccacc
     1981 aggacgcgtg ggcgtgggcg gctcggggcg tcattcccgg cggggcggcc tgacatccgt
     2041 ccgcgttcgc gcgctggaca aggcagtgac tttcgtacgc gggacagccg gaccctgccc
     2101 cttgatcggc acgcctcgga gatcgaggcg atccggagcg acgtcgcctc cggacccgcg
     2161 gtcaccccgc ccagcgaccg gacccggatc atggccggcg attcgccgcg cgacgcggcc
     2221 gccttcctcg agaaggtccg ccacgcctcg taccgggtca ccgatgacga catcgctgcg
     2281 ctgcgacgcg cgggactcgg cgaggacgag atcttcgagc tcgccgtcgc ggcgtccgtc
     2341 ggcgtcgcgc tcgcgcggct cgacgccgcg aaacgcgcca tgcggagcac cgcgtgagcc
     2401 tccgtcttgc gatcctcgag cgcggccacg cgcccgcgaa acggctgctc atccgcgcgg
     2461 cggagcgtct gggcggcgtg cgcttcgacg acgtcgcacg cacgtcgctg taccggccgg
     2521 ccttcttcgg ccgctcgtgg ctgcgcttca cgcgcagcct catgcgtggc ccgtcggagt
     2581 ggagcgccgg agaacgcgag ctcctggccg cgttcgtgtc gcgccggaac gagtgcccgt
     2641 actgcgtggg cgtgcacacc gagacggctg cgctcgggct cgcgcggccg gtcgacgtcg
     2701 cgatgctcga tgactggcgg agcgcgcccc tcgcgccgca cctgaaggcg gcgttcgagc
     2761 tgctcgagaa gcgggccgcg gatcccgcgc agctcacgcc cgcggatttc gagcgtgc
//
LOCUS       DASTZR010000441         2850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1042518, whole genome shotgun sequence.
ACCESSION   DASTZR010000441 DASTZR010000000
VERSION     DASTZR010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042518"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..587)
                     /gene="atpF"
                     /locus_tag="VFV20_09830"
     CDS             complement(66..587)
                     /gene="atpF"
                     /locus_tag="VFV20_09830"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEU5288693.1"
                     /translation="MAAEEASPFALTVDPYWIVVSIVQFLILYWLLRRFLWGPVTHAL
                     DERARKIREGLDMAESAKQERERMKQEVEQLLAQARREAGEIAERTTKAAEAAAADIK
                     TQAKVEADRTREKGRADAQALHDQALAQLRTEVASMVVLAASRILGREVSPEQHRALI
                     ERSLDEAGRELKN"
     gene            complement(603..827)
                     /gene="atpE"
                     /locus_tag="VFV20_09835"
     CDS             complement(603..827)
                     /gene="atpE"
                     /locus_tag="VFV20_09835"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEU5288694.1"
                     /translation="MNLAWLAAGIAVGFGVLGPGLGIGILTAKSSEAIGRNPDAGGAI
                     RTNMILGAALAEGLGILAFVLGILLWTKAG"
     gene            complement(856..1695)
                     /locus_tag="VFV20_09840"
     CDS             complement(856..1695)
                     /locus_tag="VFV20_09840"
                     /inference="COORDINATES: protein motif:HMM:NF012347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU5288695.1"
                     /translation="MDLLFPHGFTYPPSFHSPTLTGEPLFAIGPFSYTNAHLTMVIVG
                     AVLVALALVATRNLRERPTGLQNAVEMGVQGLSDFIIGIGGPSARKYVPLLGTFFLFI
                     LFSNWLSVVPFVGQIGFLHSPTADYHNNFAMALIAFIAYQTEGFRHLKLSYPKRWFNF
                     SGFKDGPFLGGIFVLVGLIELFSEIFRMLTLTLRLWGNVLGGEIMLVVMSGLLFLPGF
                     ALPFAGLEVFIGLVQALVFMLLVLMYHVLAVESHDEEHEEGSHADTDRVANVEQHPQP
                     LAG"
     gene            complement(1696..2022)
                     /locus_tag="VFV20_09845"
     CDS             complement(1696..2022)
                     /locus_tag="VFV20_09845"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEU5288696.1"
                     /translation="MAAPRPPGGARSNAAILGQVGITVAVPIAIGAWLGVKLDDAAGT
                     SPWGLLGLIFLGMALAGAGVWVLIKRFTEDNPVRPSTERARAAGRRWDAEIRERERKR
                     EEGEPD"
     gene            complement(2163..>2850)
                     /locus_tag="VFV20_09850"
     CDS             complement(2163..>2850)
                     /locus_tag="VFV20_09850"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU5288697.1"
                     /translation="VHKELAIFVKHNAIVTVHDGSLAALGVVERRFRDGKLATTGLLL
                     HAILDTIVDSYFDILDGLGDRVELIEAMVIEGDPRDAQTSIRELFRLKRDLLAIRKVI
                     APERDVAVALVRGDIKELRETGGRAYFQDLYDHVIRVTDEVDTFRDLVSNVVDAHLAA
                     QSNRLNEVMKVLTSIATVLLILSVVTGFFGQNWTFIPYDDQLLFWLVMATMIAAGLGT
                     AVYFKRRGWL"
BASE COUNT          496 a          935 c          964 g          455 t
ORIGIN      
        1 gggatcgccc gcgaggtcga tgagcgcctc ggcgtacctg cggccggcgg atccggaaag
       61 tgccatcagt tcttgagctc ccggccggcc tcgtcgagcg aacgctcgat gagcgcgcgg
      121 tgctgctcgg gactgacctc gcgcccgagg atgcggctcg cggcgaggac gaccatcgac
      181 gcgacctcgg tgcgcagctg cgcgagggcc tggtcgtgga gcgcctgtgc gtcggcgcga
      241 cccttctcgc gggtgcggtc cgcctccacc tttgcctgcg tcttgatgtc ggcggccgcc
      301 gcttcggcgg cctttgtcgt gcgttcggcg atctcgcccg cctcgcggcg ggcctgggcg
      361 aggagctgct cgacctcctg cttcatccgc tcgcgttcct gcttcgcgga ctcggccatg
      421 tcgaggccct cgcggatctt ccgcgcccgc tcgtcgagcg catgcgtcac cggaccccag
      481 aggaaccggc gcagcagcca gtacaggatc aggaactgga cgatcgagac gacgatccag
      541 tacggatcga ccgtcagcgc gaagggactg gcctcctcag cggccacgcc gaagagcatg
      601 acctagccag ccttcgtcca gagcaggatg ccgagcacga acgcgaggat gccgagtcct
      661 tccgcgagcg ccgcgccgag gatcatgttc gtgcggatgg cgccgcccgc gtccgggttg
      721 cgaccgatcg cctcggacga cttcgcggtg aggatgccga tgccaagacc cgggcccagg
      781 acgccgaagc cgacggcgat accggcggcg agccaagcga ggttcatcta cccgatctcc
      841 ttttaattgg tcttcttagc cagcaagtgg ctgcggatgc tgttcgacgt tcgcgacgcg
      901 atccgtatcg gcgtgactac cctcctcgtg ttcttcgtcg tggctttcga cagcaaggac
      961 gtggtacatg agcacgagca gcatgaagac gagcgcctga acgaggccga tgaagacctc
     1021 gagacccgcg aatggaaggg cgaatccggg caggaacagg agcccggaca tcacgacgag
     1081 catgatctcg ccgccgagca cgttgcccca gagacgaagc gtgagcgtga gcatgcggaa
     1141 gatctcgctg aagagctcga tgagccccac gaggacgaag atgcctccga ggaacggacc
     1201 gtccttgaag ccggagaagt tgaaccaccg cttcgggtag ctgagcttca gatggcggaa
     1261 gccctcggtc tggtacgcga tgaaggcgat gagcgccatc gcgaagttgt tgtggtagtc
     1321 ggccgtcggg gagtgcagga agccgatctg cccgacgaag ggcacgaccg acagccagtt
     1381 cgagaacagg atgaagagga agaacgtgcc gaggagcggg acgtacttgc gcgcgctcgg
     1441 cccgccgatc ccgatgatga aatccgagag accctggacg cccatctcga ccgcgttctg
     1501 cagtccggtc ggacgctcgc gcaggttccg cgtcgcgacg agcgcgagcg cgacgaggac
     1561 cgccccgacg atgaccatcg tgagatgggc attcgtgtag ctgaagggac cgatggcgaa
     1621 cagcggctca ccggtgagcg tgggactgtg gaagctcggc gggtaggtga acccgtgcgg
     1681 gaacagcaag tccacttagt ccggttcccc ctcttcccgt ttgcgttcgc gttccctgat
     1741 ctcggcgtcc cagcgccggc ccgccgcgcg cgcccgctcc gtggagggcc gcaccgggtt
     1801 gtcctccgtg aagcgcttga tgagcaccca gacaccagca cccgccaggg ccatcccgag
     1861 gaatatcaat cccagcagtc cccatggcga ggtgccggct gcgtcgtcga gcttcactcc
     1921 gagccaggct ccgatcgcta tagggaccgc gacagtgatt cctacctggc ccaggatcgc
     1981 cgcgtttgag cgcgctccac ccggggggcg gggtgcggcc aaggcacggt cagtctaccg
     2041 gaggaaacgt gttcgcgctc tgtcagccga gcgccgcgag cgggtcggcc ggcgacgcgt
     2101 cgccggcgac acccggcgag cccgcccagc gccgccgcac ccggccatcg tcgacgatcc
     2161 gtctagagcc accctcgcct cttgaagtag acggccgtgc cgagacccgc ggcgatcatc
     2221 gtggccatga ccagccagaa gagcagctga tcgtcgtacg ggatgaacgt ccagttctgc
     2281 ccgaagaagc ccgtgacgac gctgagaatg aggaggaccg tcgcgatcga ggtcaggacc
     2341 ttcatgacct cgttcaggcg attcgactgc gcggcgagat gcgcgtcgac cacgttcgac
     2401 acgaggtcgc ggaacgtgtc gacctcgtcg gtcacgcgga tgacgtggtc gtagaggtcc
     2461 tggaagtacg cgcgtccgcc ggtctcgcgg agctccttga tgtcgccgcg cacgagcgcg
     2521 accgccacgt cacgctccgg cgcgatcacc ttgcggatcg cgaggagatc acgcttcagc
     2581 ctgaagagct cgcggatgga ggtctgcgca tcgcgcgggt cgccctcgat caccatcgcc
     2641 tcgatgagct cgacccggtc gccgagcccg tccaggatgt cgaagtagct gtcgacgatc
     2701 gtgtcgagga tcgcgtggag cagcagaccc gtcgtcgcga gcttgccatc gcggaaccgc
     2761 ctttccacga cgccgagggc cgccagggat ccgtcgtgca cggtcacgat cgcgttgtgc
     2821 ttcacgaaga tcgcgagctc cttgtggacg
//
LOCUS       DASTZR010000442         2164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_938437, whole genome shotgun sequence.
ACCESSION   DASTZR010000442 DASTZR010000000
VERSION     DASTZR010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938437"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..948)
                     /gene="metF"
                     /locus_tag="VFV20_09855"
     CDS             complement(64..948)
                     /gene="metF"
                     /locus_tag="VFV20_09855"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEU5288698.1"
                     /translation="MRIAERLKTGAPAYSFEFFPPKTPEAVDRLYETVTALKKLRPTF
                     VSVTYGAGGSTRELTVDLVTRMKHELGVEAMAHLTCVGHTRDELAAVLDRLAANGIEN
                     VLALRGDPPRGQTEFVRPPGGFGHAQELARFIAERHHFCLAGAAYPEKHIEAPDLDTD
                     LAHLREKVESGVEFLITQLFFEPDRYFRFVDRARAIGIDVPIVPGIMPITNVAQIHRF
                     TAMCGASIPEALRDMLDRVKDDDTAVVALGVEWALDQCRRLLAGGAPGIHFYTLNRSH
                     SAQMILDELRTEGERALD"
     gene            1009..1737
                     /locus_tag="VFV20_09860"
     CDS             1009..1737
                     /locus_tag="VFV20_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288699.1"
                     /translation="MDRKTTAAGLRELGKEALVREPSLGTLLGRLADAVEDGRATEAS
                     GYIGAIDARGIAELLAGAHSRFWAVLDVLRNVLVFAPIAVTWAGLSLAAGAYAEMLAA
                     RPELVSQPFLLLWEQGFGGRLLFNFGTLALIDASLIGILILLSFALHLRSELRDVSFQ
                     TKVLLKESEIRALLGQAASFGALDVSGVDAESVLADMVAEERRIYERASEREGQLFQL
                     EGVVDRLRDAAIRLERAADALARR"
     gene            1745..>2164
                     /locus_tag="VFV20_09865"
     CDS             1745..>2164
                     /locus_tag="VFV20_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288700.1"
                     /translation="MRTPRRGAVSLSLAGWLWADILLGLFAIFLAASAVASAATPKAN
                     GLDARPLELRVAVSGAALLSSDPLVVSAEQDRVTADVMAQVATQRPGQRAAIVFAYGS
                     SERAADGDRMAQLATEKLTTGAFAGAILKAYHELVAGD"
BASE COUNT          296 a          754 c          781 g          333 t
ORIGIN      
        1 cagatccagc tgctccagga cgacctcgcg aagcttcgcg cgctcaccgg ccagtagccc
       61 cgctcagtcg agcgcgcgct caccctccgt ccgcagctcg tcgaggatca tctgcgccga
      121 gtgcgagcgg ttgagcgtgt agaagtggat acccggcgcg ccgcccgcga ggaggcgccg
      181 gcactggtcg agcgcccact cgacgccgag cgccacgacg gcggtgtcgt cgtccttcac
      241 gcgatcgagc atgtcgcgaa gcgcctcggg gatgctcgcg ccgcacatcg ccgtgaagcg
      301 gtggatctgc gcgacgttcg tgatgggcat gatccccggg acgatcggga cgtcgatccc
      361 gatcgcgcgc gcgcgatcga cgaagcggaa gtaccggtcc ggctcgaaga agagctgcgt
      421 gatgaggaac tcgacaccgc tctccacctt ctcgcgcagg tgcgccaggt ccgtgtcgag
      481 gtcgggcgcc tcgatgtgct tctccggata cgcggcgccc gcgaggcaga agtggtggcg
      541 ctcggcgatg aagcgcgcca gctcctgcgc gtgaccgaag cctcccggcg gccgcacgaa
      601 ctcggtctgg ccgcgcgggg gatcgccacg gagggcgagc acgttctcga tgccgttcgc
      661 ggcgagccgg tcgagcacgg cggcgagctc gtcgcgtgta tggccgacac aggtgaggtg
      721 cgccatcgcc tccacgccga gctcgtgctt catgcgcgtg acaaggtcga cggtgagctc
      781 gcgggtgctg ccgcccgcgc cgtatgtaac ggacacgaac gtcggccgca gcttcttgag
      841 agcggtcacg gtctcgtaca gccggtcgac cgcctcgggc gtcttgggcg ggaagaactc
      901 gaacgaatag gcgggtgccc ccgttttgag acgttcggcg atgcgcacgt ggcgagtatg
      961 cgggccgcag acgcacgcac gcctttactc ttgtccgcga ggaaggcagt ggatcggaag
     1021 acgaccgccg cgggtctgcg cgagctcggc aaggaggccc ttgtccgcga gccgagcctc
     1081 ggcacgctcc tcggccgcct ggccgacgcg gtcgaggacg gacgcgcgac cgaggcgagc
     1141 ggctacatcg gcgcgatcga cgcgcgcggc atcgccgagc tgctcgcggg tgcgcattcg
     1201 cgcttctggg ccgtcctcga cgtgctgcgc aacgtgctgg tgttcgcgcc gatcgcagtg
     1261 acctgggccg gtctctcgct cgccgccggc gcgtacgcgg agatgctcgc ggcgcggccc
     1321 gagctcgtct cgcagccgtt cctgctgctc tgggagcagg gcttcggcgg acggctgctc
     1381 ttcaacttcg gcacgctcgc gctcatcgac gcctcgctca tcgggatcct catcctgctg
     1441 tcgttcgcgc tgcacctgcg gagcgagctg cgcgacgtgt cgttccagac gaaggtcctg
     1501 ctcaaggagt ctgagatccg cgcgctgctc ggccaggccg cgtccttcgg cgcgctcgac
     1561 gtctccggcg tcgatgccga atcggtgctg gccgacatgg tcgccgagga gcggcgcatc
     1621 tacgagcgcg cgtccgagcg tgaggggcag ctcttccagc tcgagggtgt cgtcgatcgc
     1681 ctgcgcgacg cggcgatcag gctcgagcgc gccgccgacg ccctcgcgcg ccgctaaggg
     1741 accgatgcgc acgccgcgtc gcggtgccgt ctcgctcagc ctcgccggat ggttgtgggc
     1801 cgacatcctc ctcgggctct tcgcgatctt cctcgcggcg agcgcggtcg cgtccgcggc
     1861 gacgccgaag gcgaacgggc tcgacgcgag accgctcgag ctgcgcgtcg cggtgagcgg
     1921 cgcggccctg ctgtcgtcgg atccgctcgt cgtctccgcg gagcaggacc gcgtcaccgc
     1981 cgacgtgatg gcgcaggtcg cgacccagcg gccggggcag cgcgcggcga tcgtcttcgc
     2041 atacggctcg agcgagcgcg ccgccgacgg cgaccgcatg gcgcagctcg ccaccgagaa
     2101 gctcacgacc ggcgcgttcg cgggcgcgat cctgaaggcg taccacgagc tcgtcgcggg
     2161 agac
//
LOCUS       DASTZR010000443         2715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_410414, whole genome shotgun sequence.
ACCESSION   DASTZR010000443 DASTZR010000000
VERSION     DASTZR010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410414"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..493
                     /locus_tag="VFV20_09870"
     CDS             170..493
                     /locus_tag="VFV20_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288701.1"
                     /translation="MRRKIEDERDARACLKAAKAARLSAGEWAHQNGVDGRSLNAWRI
                     NLSHRALRGSATGTTPRLVELVSASVPRVETRYVVRVAGAEIEVTDAFHEQTLVRLVR
                     ALRSC"
     gene            487..849
                     /gene="tnpB"
                     /locus_tag="VFV20_09875"
     CDS             487..849
                     /gene="tnpB"
                     /locus_tag="VFV20_09875"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU5288702.1"
                     /translation="MLSLPPTVRVFVAVEPIDMRGSFDALAGAVRRLGLDPVDGHLYL
                     FLNKRRRIAKALWFDGSGWCLLAKRLEAGSFQLPPLETAENQVQIDGATFASLLAGID
                     FTAARRGWYRRLPSRSGR"
     gene            889..2415
                     /locus_tag="VFV20_09880"
     CDS             889..2415
                     /locus_tag="VFV20_09880"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEU5288703.1"
                     /translation="MHVDVDALLQENAAQRAQLAEVIATNTKLVGEIAKLNDRVAELL
                     AIAQRKQRKTTTPAEKKPEAPPAVAADAQRAFEARPKPPALPEKTKPSKGGARRTGRS
                     AIPAHLEAEEHRFRPTHCEHCGSEELNIADEVVEEKLHVVKEHQRRRVVRRTTCRCRT
                     CLKRSTPASLPAPYARSKVTCEWLAWFIDQKFKLLSPLDRIRRDLAEREIHLAMGTLV
                     SFVERAADLLAPIDGLHWKKLLSSSWMATDGTGLKVLVPGLPNAHDGYVELYRNAECA
                     VFQYAAHKGSEDVVAKLAPFRGTLTADAEHRFNAVYATGRVIEAGCNAHGRRKFRDAE
                     STQPVLAKEGGAFIAAMYVAEEDARKNDLVGDALLAHRRDHIRPIADAFAKWLAAVEP
                     TLLPSDPLTAAVGYYRKHWDALTRFIDDAQVPIDNSPTEREFQNFAKLRLNMLFAGSS
                     EGAHRACVLLGIVATCRAIGVPVQAYLAWAFERLGTHREKFGLPLEQITPAAFKAARG
                     "
BASE COUNT          445 a          927 c          933 g          410 t
ORIGIN      
        1 cgccgcactc ggggtgatgc tcaggtgatg gtgtgtgatg acacgtacgc cgtggtgcac
       61 gtcgaacccg agcacgcgct gcgcatcggc gagcgcatac agccagcact gtgaccgctc
      121 agcgacggcg ggatgacctg agcggcgccc gcggtcaacg tcgcgcgcga tgcgacgcaa
      181 gatcgaagac gagcgggatg cgcgtgcgtg cctgaaggcg gcgaaggcgg cgaggctcag
      241 cgccggggag tgggcgcacc agaacggggt cgacggccgc tcgctcaacg cgtggcggat
      301 caatctctcg caccgcgcgc tgcgggggag cgcgacgggc acgacaccgc ggctggttga
      361 gctggtgtct gcgtcggtcc ctcgcgtcga gactcgatat gtcgtgcgcg tcgccggggc
      421 cgagatcgag gtaacggatg cgttccacga acagaccctc gtgcgcctcg tgcgggcgct
      481 gcgttcgtgc tgagtctgcc gcccacggtt cgcgttttcg tcgccgtcga gccgatcgac
      541 atgcgcggct ccttcgacgc gctcgccggc gccgttcgac ggctggggct cgatcccgtc
      601 gacggacacc tttacctgtt cctgaacaag cggcgacgga tcgcgaaggc gctctggttc
      661 gatgggtcgg gctggtgcct gctggcaaag cgattggagg ccgggagctt tcagctgcct
      721 ccgctcgaga ccgcggaaaa ccaggttcag atcgatggcg cgacgttcgc ctcgttgctc
      781 gcgggcatcg acttcacggc ggcgcgtcgc ggctggtacc gacggcttcc gagtcgttcg
      841 ggtcgatagg ggatcgacgc cgatcgcgag ctgtgatctc gtcacgtcgt gcacgtcgac
      901 gtcgacgcac tgctccagga gaacgccgcg cagcgggcgc agctcgccga ggtgatcgcg
      961 acgaacacga agctcgtggg cgagatcgcg aagctcaacg atcgcgtcgc ggagctattg
     1021 gcgatcgctc agcgcaagca gcgcaagacg accacgcccg cggagaagaa gcccgaggcg
     1081 ccgcctgcgg tcgcggccga cgcgcagcga gccttcgaag cgcgacccaa gccgcccgcg
     1141 ctgccggaga agacgaagcc atcgaagggc ggcgcgcgtc gcaccggtcg aagcgcgata
     1201 cccgcgcatc tcgaggcgga ggaacatcgc ttccggccga cgcactgcga gcactgcggc
     1261 tcggaggagc tgaacatcgc ggacgaggtc gtcgaagaga agctgcacgt cgtcaaggag
     1321 caccagcgcc gtcgtgtcgt gcggcgcacg acgtgtcgct gtcggacatg tctgaagcga
     1381 tcgacgcccg cgtcgctgcc cgcgccgtac gcgcggtcca aggtgacctg cgagtggctc
     1441 gcatggttca tcgatcagaa gttcaagctg ctctcgccgc tcgatcggat ccgccgcgac
     1501 ctcgcggagc gcgagatcca cctcgcgatg ggcacgctcg tgagcttcgt cgagcgcgcg
     1561 gccgacctgc tcgcgccgat cgacggcttg cactggaaga agctcctgtc gagctcgtgg
     1621 atggctaccg acggcacggg cctcaaagtg ctcgtgcccg gtctgcccaa cgcgcacgac
     1681 ggttatgtcg agctctaccg caacgcggag tgcgcggtat tccagtacgc agcgcacaag
     1741 gggagcgagg acgtcgtcgc caagctcgcg ccgttccgag gaacgctcac tgccgacgcc
     1801 gagcaccgct tcaacgccgt ctacgcgacg ggccgcgtga tcgaggccgg ctgcaacgcg
     1861 cacggtcgcc gcaagttccg cgacgcggaa tcgacgcagc ccgtgctcgc gaaggagggc
     1921 ggcgcgttca tcgcggcgat gtacgtcgcc gaagaagacg cgcgcaagaa cgacctcgtc
     1981 ggagacgcgt tgcttgcgca tcgacgcgat cacatccggc cgatcgccga cgcgttcgcg
     2041 aagtggctcg ccgccgtcga gccgacgctc ttgccctccg acccgctcac cgcagcggtc
     2101 ggttactacc gcaagcattg ggatgcgctc acccgcttca tcgacgacgc gcaggtgccg
     2161 atcgacaact cgcccaccga gcgcgagttc cagaacttcg cgaagctccg cctcaacatg
     2221 ctcttcgccg gcagcagcga gggggcgcac cgcgcctgtg ttctgctcgg catcgtcgcg
     2281 acgtgccgtg cgatcggcgt tcccgtccaa gcctacctag cgtgggcctt cgagcgcctc
     2341 ggcacgcatc gcgagaagtt cgggcttccg ctcgagcaga tcacgcccgc cgccttcaaa
     2401 gccgcgcgcg gctgacggcc gcgccgcccg gtggcacgcc ccaactctgt tcgcatcccg
     2461 gcttggctga tcggtcacgc accatgccgc tcggacgtcc tcgagcagcc ccgcgcgcag
     2521 ctcggtgcgg ggcggggtat cttcgccgcg gcgaagctca tccatcacct cgcacaagag
     2581 cgggcccggc tgagcgagga tcgcgtgggg cagtggatcc gataccggcg cgttgtggaa
     2641 gacccgcatc gcatacgtcc caaacggaaa cgcggcgtct tctcccgcgc aacgccgggc
     2701 ctggacggtt ccacc
//
LOCUS       DASTZR010000444         2585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_101349, whole genome shotgun sequence.
ACCESSION   DASTZR010000444 DASTZR010000000
VERSION     DASTZR010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101349"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VFV20_09885"
     CDS             complement(<1..730)
                     /locus_tag="VFV20_09885"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEU5288704.1"
                     /translation="MSTGVVLMTYGAPHGEADLPRYLAAVRGGRAADPALVAEMARRY
                     AVIGGSPLVERTVAQAVSLERELGGGYRCVAGMRFSPPTVADAVGSLAAAGATRVVGI
                     CMSPQWSPLLMGGYERELRAAADAAGCGAAIAGAWHREPLFIASLAERTRDALRGLDD
                     PFVLLTAHSLPKRVFDAEPGYIRQLRETGDLVAAAAGLHGDRWRWAYQSAGHTQEEWL
                     RPDLVELFPDIVATGTREILVVPVQ"
     gene            complement(727..1764)
                     /gene="hemE"
                     /locus_tag="VFV20_09890"
     CDS             complement(727..1764)
                     /gene="hemE"
                     /locus_tag="VFV20_09890"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEU5288705.1"
                     /translation="MTASLIVRALRREPVERTPVWYMRQAGRCLAEYRALRQRYGILE
                     MAKTPELCARVTRMPVDRLGVDAAVLYADIMLPLDGMGVPFHIKPDIGPIVERPVRTP
                     EDVAALRVIDAREATPYLFETIRALRADLPAEIALVGFCGAPFTLASYLIEGGPSRDH
                     AKAKAMLLAAPELWGRLMDTLVEVLSRYLAAQIAAGVDVVQVFDSWAGTLSTTDYERS
                     VLPWSRRLFASLSGTGVPRIHFATGNPALLPLIASVECEAVSVDWRAPLDDAWRAIGD
                     RAIQGNLEPAVCLAPWEVVEARALDVLRRAGGRPGHVFNLGHGVLAETDADVLARLAA
                     LVHERTAAVPA"
     gene            complement(1761..2468)
                     /locus_tag="VFV20_09895"
     CDS             complement(1761..2468)
                     /locus_tag="VFV20_09895"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEU5288706.1"
                     /translation="MNQLFRYLALAVTPEWRRLPEDERTEHTRELARAARHDAVRVHA
                     YSLVGTRADADLLLWCVAEDLEPLRDLETRLAGTRLWSYATRPYAYLAARRRSPYLGE
                     HAHAGAESARADAGPVGDTPYVVVYPMTKKRSWYALPREERTRIMAGHFAVGHRYPDI
                     RIHTAYSFGIDDHEFVVSFEVPDVRRFLALVADLRETESSAYTEKETPIFVGAAMPLE
                     RALDEIDGAAARAEIAS"
BASE COUNT          390 a          920 c          968 g          307 t
ORIGIN      
        1 actgcacggg gacgacgagg atctcgcgcg tcccggtggc cacgatgtcg gggaagagct
       61 cgaccagatc cgggcgcagc cactcctcct gcgtgtgtcc cgcgctctga taggcccagc
      121 gccagcgatc gccgtggagg ccggctgcgg cagcgacgag gtctcccgtc tcgcgcagct
      181 gccggatgta gccgggctcg gcgtcgaaca cgcgcttcgg caggctgtgc gcggtgagca
      241 gcacgaacgg gtcgtcgaga ccgcggagcg cgtcgcgcgt gcgctccgcc agcgacgcga
      301 tgaagagcgg ctcgcggtgc caggcaccgg cgatcgccgc accgcacccg gcggcatccg
      361 ccgcggcgcg cagctcgcgc tcgtagccac ccatgagcag gggcgaccac tgcggcgaca
      421 tgcagatccc cacgacacgc gtggcgccgg ccgcggcgag cgagccgacc gcgtccgcga
      481 ccgtgggcgg cgagaagcgc atccccgcga cgcagcggta gccgccgccg agctcgcgct
      541 cgagcgacac cgcctgcgcg accgtgcgct cgacgagcgg cgagccgccg atgaccgcgt
      601 agcggcgcgc catctcggcg acgagcgcgg ggtcggccgc gcgtccgccg cggacggcgg
      661 ccaggtagcg cggcagatcc gcctcaccgt gcggcgcgcc gtaggtcatg aggacgacgc
      721 cggtgctcac gccggcaccg ccgccgtgcg ctcgtgcacg agcgcggcaa ggcgcgcgag
      781 gacgtcggcg tccgtctcgg cgagcacgcc gtgaccgagg ttgaagacgt ggcccggccg
      841 gccgccggca cgccgcagca catccagcgc acgcgcctcg acgacctccc acggcgcgag
      901 gcagaccgcg ggctcgaggt tgccctggat ggccctgtcg ccgatggcgc gccacgcgtc
      961 gtcgagcggc gcgcgccagt ccacgctcac ggcctcgcac tcgaccgacg cgatgagcgg
     1021 cagcagcgcc ggattgcctg tcgcgaagtg gatgcgcggc acgcccgtac cggagagcga
     1081 cgcgaagagc cgccgcgacc acggcaggac cgagcgctcg tagtcggtgg tcgacagcgt
     1141 ccccgcccac gaatcgaaga cctggaccac gtcgacgccg gcggcgatct gcgcggcgag
     1201 ataccgcgag agcacctcca ccagcgtgtc catgagccgc ccccacagct ccggcgcggc
     1261 gagcagcatc gccttggcct tcgcgtgatc gcgcgacggc ccgccctcga tgagatacga
     1321 cgcgagggtg aatggcgcac cgcagaaacc gacgagcgcg atctccgcgg gcaggtctgc
     1381 gcgcagggcg cggatggtct cgaagagata cggcgtcgct tcccgcgcgt cgatcacgcg
     1441 gagcgcggcc acgtcctcgg gcgtgcgcac ggggcgctcg acgatcggtc cgatgtccgg
     1501 cttgatgtgg aacggcacgc ccatgccgtc gagcggaagc atgatgtccg cgtagagcac
     1561 ggccgcgtcg acgccgaggc gatcgaccgg catgcgcgtc acgcgcgcgc agagctcggg
     1621 cgtcttcgcc atctcgagga tgccgtagcg ctggcgcaag gcgcggtact cggcgaggca
     1681 gcggccggcc tgccgcatgt accagaccgg cgtccgctcc acgggctcgc gacggagcgc
     1741 gcggacgatg agcgacgccg tcatgaggcg atctccgcgc gtgccgccgc gccgtcgatc
     1801 tcgtcgagcg cgcgctcgag cggcatcgcg gcgccgacga agatgggcgt ctccttttcc
     1861 gtgtacgccg acgactccgt ctcgcgcagg tccgcgacga gcgcgaggaa gcggcggacg
     1921 tcgggcacct cgaaggagac cacgaactcg tggtcgtcga tgccgaagct gtacgcggtg
     1981 tggatacgga tgtcgggata gcgatggccg accgcgaagt gcccggccat gatgcgcgtg
     2041 cgctcctcgc gcgggagcgc gtaccacgag cgcttcttcg tcatcggata gacgaccacg
     2101 tacggggtgt cccccaccgg ccccgcgtcg gcgcgcgccg actccgcgcc ggcatgcgcg
     2161 tgctcgccca ggtacggaga gcggcgccgg gccgcgaggt aggcgtaggg ccgcgtcgcg
     2221 taggaccaga gccgcgtgcc ggcgaggcgc gtctcgagat cacgcagcgg ctcgaggtcc
     2281 tcagcgacgc accagagcaa caggtcggcg tcggcgcgcg tgccgacgag cgagtacgca
     2341 tggacgcgca cggcgtcgtg acgcgcggcg cgcgcgagct cgcgcgtgtg ctcggtgcgc
     2401 tcgtcctcgg ggagccgccg ccactcgggc gtgaccgcga gcgcgaggta gcggaagagc
     2461 tgattcatgc gacgatctcc ttgagggtcg atccggccgc cgcgacgatc gcgtcgatgt
     2521 cggcatcggt atgcgcgagc gagaggaaga ggcattcgaa ctgcgacggc gggagcagga
     2581 cgccg
//
LOCUS       DASTZR010000445         2702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_410439, whole genome shotgun sequence.
ACCESSION   DASTZR010000445 DASTZR010000000
VERSION     DASTZR010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410439"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..1045)
                     /locus_tag="VFV20_09900"
     CDS             complement(104..1045)
                     /locus_tag="VFV20_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5288707.1"
                     /translation="MGLLEGKVAIVTGAGRGIGREHALALAREGAKVVVNDLGATLQG
                     EGTDESPAHDVVREIEAIGSAAVADGGNVASWSDAEAMVKRALDEFGRLDILVNNAGI
                     TRDRMLVNMSEEEWDSVITVHLKGHFAPTRHAAAHWRELSKAGEEVRGRVINTASPSG
                     VFGNVGHANYGAAKAGIAGFTIIAAQELARYGVTVNCLAPNARTRMTEAAFGEIPKPE
                     DGFDAMDPANMAPIVVALCADEAQNVTGQCFFVYGGAVNVLRGWDSGELLERDGRWDA
                     DDFLSQLTERFPDGLAPEGMAALMAKAGGHSLRAASN"
     gene            complement(1047..1493)
                     /locus_tag="VFV20_09905"
     CDS             complement(1047..1493)
                     /locus_tag="VFV20_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018087855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEU5288708.1"
                     /translation="MKIAELKDHVGKELGASSWHEITQERVNTFAEATDDHQWIHVDP
                     EMAAQGPFGTTIAHGYLTLSLAPALMSEIWEFEDVQMGINYGLNRVRFPAPVPVGSKV
                     RAVAKLQSVEEIPNGVQIVVECTFEREGGDKPVCVAETVSRLYGGE"
     gene            complement(1490..1939)
                     /locus_tag="VFV20_09910"
     CDS             complement(1490..1939)
                     /locus_tag="VFV20_09910"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEU5288709.1"
                     /translation="MSVTKEDVVARMRRDLDPDEYTAIRELWKSHSLAEDARDLPGLI
                     ATLTEDCVYELAGTEHRWEGHEGAARFYTELLTAFPDIHFDLTDIVIGPQGVCEEATV
                     TGTHKAAWLGNEPTGEKLEWKVAIFFPWDADARKFKGEKVYTAGLPA"
     gene            complement(1936..2583)
                     /locus_tag="VFV20_09915"
     CDS             complement(1936..2583)
                     /locus_tag="VFV20_09915"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU5288710.1"
                     /translation="MRHGEVAYFDAAGKPVAPESVGLTAHGEEQAAAARRALQHVAFD
                     RVITSGLVRTMETARIVAPGFELETRPELEELRGGRLEDIPDEELDEAFAHAFRGIHP
                     PDARFLGGESTQEFLDRVIPAFERFVDEDWDTALAVLHGGVNRAILSYALTGERVMLG
                     NFEQEAGCINIVDLDEGRWIVRTTGYAPLDPVHEERLTVMERYLEQYLPYRRSTT"
BASE COUNT          355 a          993 c          905 g          449 t
ORIGIN      
        1 agtcgatccg ccgttggtcg atcgtcaccc agtcggtcgt tccgaggtcg cgctcctgcg
       61 tgctctcgag ctcatccagc gtcagcgtcg tcatcgctct cctctagttc gaggccgcgc
      121 ggagcgagtg cccgccggcc ttggccatca gcgccgccat cccctccgga gcgagcccat
      181 ccgggaagcg ctcggtgagc tgcgacagaa agtcgtcggc gtcccagcgc ccgtcgcgtt
      241 cgagcagctc gcccgagtcc catccgcgca ggacgttgac cgcaccgccg tacacgaaga
      301 agcactggcc cgtcacgttc tgcgcctcgt ccgcgcacaa cgcgacgacg atcggcgcca
      361 tgttggcggg atccatggcg tcgaagccgt cctccggctt cggaatctcg ccgaacgcgg
      421 cttccgtcat gcgcgtgcgc gcgttcgggg cgaggcagtt gacggtgacg ccgtagcggg
      481 cgagctcctg cgcggcgatg atcgtgaagc ctgcgatgcc cgccttcgcg gcgccgtagt
      541 tcgcatggcc cacgttgccg aagacgcccg acggcgacgc cgtgttgatc acacggccgc
      601 gcacctcctc gccggccttc gacagctccc gccagtgcgc cgccgcgtgc cgcgtcggcg
      661 cgaagtggcc cttgaggtgg acggtgatga ccgagtccca ctcctcctcg ctcatgttca
      721 cgagcatgcg gtcgcgggtg atgccggcgt tgttgacgag gatgtcgagc cggccgaact
      781 cgtcgagcgc gcgcttcacc atcgcctcgg cgtccgacca cgacgcgacg ttgccgccgt
      841 cggcgaccgc cgcgctgccg atcgcctcga tctcgcgcac cacgtcgtgc gcgggagact
      901 cgtccgtgcc ctcgccctgg agcgtcgcgc cgaggtcgtt gacgacgacc ttcgctccct
      961 cgcgtgccag cgccagcgcg tgctcgcggc cgatgccgcg gccggcgccc gtgacgatcg
     1021 caaccttgcc ctccagtaga cccatgtcac tctcctccgt agagacggga aacggtttcc
     1081 gcgacgcaga ccggcttgtc gccgccctcg cgctcgaacg tgcactcgac gacgatctgg
     1141 acgccgttcg ggatctcctc gaccgactgc agcttcgcga cggcgcgcac cttcgacccc
     1201 accgggacgg gcgccgggaa gcgcacgcgg ttgaggccgt agttgatccc catctgcacg
     1261 tcctcgaact cccagatctc gctcatcagc gccggcgcga gcgagagcgt caggtagccg
     1321 tgggcgatcg tcgtcccgaa cggcccctgc gcggccatct ccgggtcgac gtggatccac
     1381 tggtggtcgt cggtcgcctc ggcgaacgtg ttcacccgct cctgcgtgat ctcgtgccac
     1441 gagctcgcgc cgagctcctt ccccacgtgg tccttcagct cggcgatctt catgccggca
     1501 gccccgccgt gtagaccttc tcgcccttga acttgcgcgc gtccgcgtcc caggggaaga
     1561 agatcgcgac cttccactcg agcttctcgc ccgtcggctc gttgccgagc cacgccgcct
     1621 tgtgcgtgcc cgtcacggtc gcctcctcgc acacgccctg cgggccgatg acgatgtcgg
     1681 tcaggtcgaa gtggatgtcc gggaacgcgg tcaggagctc cgtgtagaag cgggccgctc
     1741 cctcgtggcc ctcccagcgg tgctctgtgc ccgcgagctc gtagacgcag tcctcggtca
     1801 gggtcgcgat caggcccggg aggtcgcgtg cgtcctcggc cagcgagtgc gacttccaga
     1861 gctcgcggat cgcggtgtac tcgtcggggt cgaggtcacg ccgcatgcgt gcgacgacgt
     1921 cctccttggt cacgctcatg tggtgctcct tcggtagggg aggtactgct ccaggtagcg
     1981 ctccatcacg gtcagccgct cctcgtgcac cgggtcgagc ggggcgtagc cggtcgtgcg
     2041 cacgatccag cggccctcgt cgaggtcgac gatgttgatg cagccggcct cctgctcgaa
     2101 gttgccgagc atcacgcgct cgccggtcag ggcatacgag aggatcgccc ggttgacgcc
     2161 gccgtgcagg acggcgagcg cggtgtccca gtcctcgtcg acgaagcgct cgaacgccgg
     2221 gatcacgcga tcgaggaact cctgcgtcga ctcgccgccg aggaagcgcg cgtccggcgg
     2281 gtggatgccc cggaaggcgt gcgcgaaggc ctcgtcgagc tcctcgtcgg ggatgtcctc
     2341 gagccggccg ccgcgcagct cctcgagctc cggccgcgtc tcgagctcga acccgggcgc
     2401 gacgatgcgc gcggtctcca tcgtgcggac gagcccgctc gtgatcacgc ggtcgaacgc
     2461 gacgtgctgg agcgcgcgcc gcgccgccgc agcctgctcc tcgccgtggg cggtgaggcc
     2521 gacgctctcg ggcgccaccg gcttcccggc cgcgtcgaag tacgccacct cgccgtggcg
     2581 catgaggtag aggcgcctac gcgacgagct cagcgaggac ctccagcgcc tccggctgcc
     2641 gcgcctgcga cgtgatggtc gtgacgccgc tctcgcgcca ggcggcgagc cggtcggcga
     2701 tg
//
LOCUS       DASTZR010000446         3505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_259401, whole genome shotgun sequence.
ACCESSION   DASTZR010000446 DASTZR010000000
VERSION     DASTZR010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259401"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..1567)
                     /locus_tag="VFV20_09920"
     CDS             complement(152..1567)
                     /locus_tag="VFV20_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020473400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5288711.1"
                     /translation="MPDAVVVGSGPNGLAAAIHVAQAGKSVTVYEANDDIGGGARSKE
                     LTLPGFVHDVCSAFHPMAAASPFFRTLRLGEHGLRWIHPTAPLAHPFDDGTAVILERS
                     LDDTVRALGRDGKAYAALMGPLVGSWSDLYDEVLGPIGHVPRHPLLLARFGLDAMRSA
                     RSLVSRFREGRARALLAGIAAHSFLPLEAPFSASAALLLGAAGHAVGWPIARGGSGAI
                     ATALASHLRALGGVVTTGHRVQELSDVPTAAAYLFDTSPAQLDMIAADRLSPGYRRAL
                     RRYRRGPGVFKVDYALDGPIPWHAEACVRAGTVHLGGTFEEIAASERAVSRGDHPDRP
                     FLLVGQQSLFDDTRAPAGKHTVWAYCHVPNGSSEDMTTRIEAQLERFAPGFRERVLAR
                     AVTTPADIERANANDLGGDIANGSHSGLQLLMRPRVALDPYATSARDIYLCSAATPPG
                     GGVHGMCGYHAARSALRRALR"
     gene            complement(1560..>3505)
                     /locus_tag="VFV20_09925"
     CDS             complement(1560..>3505)
                     /locus_tag="VFV20_09925"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU5288712.1"
                     /translation="AEDWKARGTERPPTTIANPAADIVPVAGAFRPRSIGEQFTRVPI
                     EDLVGSPYVDWQWGGVSLSPDSTEVAFAWNKTGTFEIYSAPIEKERLYQLTDAKDRSV
                     WPRWSPDGKQIAFLRDHGGDERFDIWVVDREGEHERDLTGEPKVMHREIAWSPDGGRI
                     AYAANASGKGFAIHVLDVATGEKRALTDGAHDDHSPRWSPDGARLVFWSRRESVRTNA
                     ELYLVAAAGGAVTRLDTRGGAEGESIDGRFSPDGTRIAFTTNTRGRQEVAIAHLEGDR
                     VARIERLTENIHDETEPVWRPDGRGLIFRHSEESEISLRRVFAVSHATHAVADRPGVH
                     AWANVGADSDLVAYAYTSAREPWDVYVTRERLVAPKRLTRSLPATVDPATLVEPVHVR
                     YPGADGREIPALLYVPYTEAIRGGSPPPAVVHVHGGPTGQHLRWWDRHSQYFANNGYV
                     VLAPNIRGSTGYGREFQEANRGDWGGKDLRDVVLGAQWLAKQGIADPKRIGVYGGSYG
                     GFMTLMVLTQAPDVWAAGVSIVGVVSWKTLWETTRGDLKDYLERELGDPAKAADLYRD
                     RSPLTHVSKIKAPLLLLQGENDPRVPRSEADQMIDALREGGKTFEEHIYAGEGHGFRT
                     TENMIDSLRRATEWFDRHMGARA"
BASE COUNT          487 a         1242 c         1280 g          496 t
ORIGIN      
        1 cggaccgccg ggtatgcgcg cccgcgagac gctcgcgcgc cacggcgacg agctgcggcc
       61 acgattcgag gtcgcgttgg ccggccagga cttcgcgctg tacacgagcg agacgctacg
      121 gagacgttcc tagagcccgg ctagggcttg gctagcgaag cgcgcgccgc agcgcgctgc
      181 gcgccgcgtg atagccgcac atcccgtgca cgccgccgcc gggcggggtc gccgcgctgc
      241 acaggtagat gtcgcgcgcg ctcgtggcgt acgggtcgag cgcgacgcgc gggcgcatga
      301 gcagctgcag cccgctatgt gatccgttcg cgatgtcccc gccgaggtcg ttcgcgttcg
      361 ctcgctcgat gtccgccggc gtcgtcaccg cgcgggcgag gacgcgctcg cggaatccgg
      421 gcgcgaagcg ctcgagctgc gcctcgatgc gcgttgtcat gtcctcgctc gatccgttcg
      481 gaacgtggca gtaggcccac acggtgtgct tcccggccgg cgcgcgagtg tcgtcgaaga
      541 ggctctgctg gccgaccagc aggaacggac ggtcgggatg gtccccgcgc gacaccgcgc
      601 gctcgctcgc ggcgatctcc tcgaaggtgc cgccgagatg gacggtaccg gcccggacgc
      661 aggcctccgc gtgccagggg atcggtccgt cgagcgcgta atccactttg aagacgccgg
      721 ggccgcgccg gtagcggcgc agtgcgcgcc ggtatcccgg ggagagccgg tccgcggcga
      781 tcatgtcgag ctgcgcgggc gacgtgtcga agagataggc cgccgcggtc gggacatccg
      841 acagctcctg tacacgatgg ccggtggtca cgacgccgcc gagcgcgcgc aggtgcgacg
      901 cgagcgcggt cgcgatcgcg cccgagccgc cgcgcgcgat gggccacccc accgcgtgac
      961 ctgccgcgcc gaggaggagc gcggccgacg cgctgaaggg cgcctcgagc ggaaggaagg
     1021 agtgcgcggc gatcccggcg aggagcgccc gggcgcggcc ctcgcggaac cgcgacacga
     1081 gcgaacgcgc ggaccgcatc gcatcgaggc cgaagcgcgc cagcaggagc ggatgccgcg
     1141 gcacgtgccc gatcggaccg aggacctcgt cgtaaagatc gctccacgac ccgacgagcg
     1201 gccccatgag cgcggcgtac gccttgccgt cgcggccgag cgcgcggacg gtgtcgtcga
     1261 gcgaccgctc gaggatgacg gcggtgccgt cgtcgaacgg gtgcgcgagc ggcgcggtcg
     1321 gatggatcca ccggagcccg tgctcgccca ggcgcagcgt ccggaagaac ggcgatgcgg
     1381 cggccatagg gtggaacgcc gagcagacgt cgtggacgaa gccggggagc gtcagctcct
     1441 tggagcgcgc gcctccgccg atgtcgtcgt tcgcctcgta gaccgtgacg ctcttgccgg
     1501 cctgcgcgac atggatcgcc gcggcgaggc cgttcggccc cgagccgacg acgaccgcat
     1561 caggcacgcg cccccatgtg acggtcgaac cattcggtcg cgcggcggag cgagtcgatc
     1621 atgttctcgg tcgtgcggaa gccgtggccc tcgccggcgt agatgtgctc ctcgaacgtc
     1681 ttcccgccct ctcgcagcgc gtcgatcatc tgatccgcct cggagcgcgg cacgcgcggg
     1741 tcgttctcgc cctgcagcag cagcagcggc gccttgatct tcgacacgtg cgtgagcggc
     1801 gagcgatcgc ggtagaggtc cgccgccttc gcgggatcgc caagctcgcg ctcgaggtag
     1861 tccttgaggt cgccgcgcgt cgtctcccac agcgtcttcc acgacacgac gccgacgatg
     1921 gagacgccgg cggcccagac gtcgggcgcc tgcgtgagga ccatcagcgt catgaacccg
     1981 ccgtagctgc cgccgtagac gccgatgcgc ttcgggtccg cgatcccctg cttcgcgagc
     2041 cactgcgcgc cgaggacgac gtcgcgcaga tccttgccgc cccagtcgcc gcggttggcc
     2101 tcctggaact cgcgtccgta tccggtcgat ccgcggatgt tcggcgcgag caccacgtag
     2161 ccgttgttcg cgaagtactg gctgtggcgg tcccaccacc gcaggtgctg gccggtgggg
     2221 ccgccgtgga catggacgac ggccggcggc ggagagccgc cgcggatcgc ctccgtgtac
     2281 ggcacgtaga gcagcgccgg gatctcgcgg ccgtccgcgc cgggatagcg gacgtgcacc
     2341 ggctcgacga gcgttgccgg atcgaccgtc gcggggagag atctggtgag ccgcttcggc
     2401 gcgacgagac gctcgcgcgt gacgtacacg tcccacggct cccgcgcgct cgtgtacgcg
     2461 tacgccacga ggtccgagtc ggcgccgacg ttcgcccagg catgcacgcc gggacgatcg
     2521 gccaccgcat gggtcgcatg tgacaccgcg aagacacggc gaagcgagat ctcggactcc
     2581 tcgctgtgcc ggaagatgag cccgcggccg tccggccgcc agaccggctc cgtctcgtcg
     2641 tggatgttct ccgtgagccg ctcgatgcgc gcgacacggt cgccttcgag atgcgcgatg
     2701 gcgacctcct ggcggccgcg cgtgttcgtg gtgaacgcga tgcgcgtgcc gtcgggagag
     2761 aagcggccgt cgatcgactc gccctccgcg ccgccgcgcg tgtccagtcg cgtcacggca
     2821 ccgccggcgg cggcgaccag gtacagctcg gcgttcgtgc gcacgctctc gcggcgggac
     2881 cagaacacca ggcgcgcgcc gtccggcgac catcgcggcg agtgatcgtc gtgcgcgccg
     2941 tccgtcagcg cgcgcttctc gccggtcgcg acgtccagga cgtggatcgc gaatcccttg
     3001 cccgacgcgt tcgcggcgta ggcgatgcgg ccgccgtcgg gcgaccacgc gatctcgcgg
     3061 tgcatgacct tcggttcgcc ggtgaggtcg cgctcgtgct cgccctcgcg atcgacgacc
     3121 caaatgtcga agcgctcgtc gccgccgtgg tcgcggagga aggcgatctg cttcccgtcc
     3181 ggcgaccagc gcggccacac gctgcggtcc ttcgcgtcgg tgagctggta gagacgctcc
     3241 ttctcgatcg gcgcgctgta gatctcgaac gtgccggtct tgttccaggc gaacgcgacc
     3301 tcggtcgagt cgggcgacag gctcacgccg ccccactgcc agtcgacata cggcgacccg
     3361 acgagatcct cgatgggcac gcgcgtgaac tgctccccga tcgagcgcgg gcggaacgca
     3421 ccggcgaccg gcacgatgtc cgcggcggga ttcgcgatgg tcgtcggcgg acgctcggtg
     3481 ccacgggcct tccagtcctc cgcga
//
LOCUS       DASTZR010000447         2078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_984591, whole genome shotgun sequence.
ACCESSION   DASTZR010000447 DASTZR010000000
VERSION     DASTZR010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984591"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VFV20_09930"
     CDS             <1..370
                     /locus_tag="VFV20_09930"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEU5288713.1"
                     /translation="HNFAFLGPESNWAAEAALCAGEQGKFFEFHDRLFASQAGENKGA
                     FAKDKLKRMGTDLGLSPSFAACVDSGKYAQSVRDEQNVGQGKGVSKTPTHFVNGKKYE
                     GAYSFDEMKNIIDPLLAGGR"
     gene            373..783
                     /locus_tag="VFV20_09935"
     CDS             373..783
                     /locus_tag="VFV20_09935"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEU5288714.1"
                     /translation="MSRRAALVFATIGTAVSAYLTWIHYSGAFAFCAGVGDCEVVQTS
                     RYSMLGPIPVALIGLAGMVLIFGVSLGRLVSDDPRLDLALFALALMATAYVAYLSYVE
                     VFVLGAICPWCVAVAVCSVAIFALAARDVMSASS"
     gene            complement(764..1639)
                     /locus_tag="VFV20_09940"
     CDS             complement(764..1639)
                     /locus_tag="VFV20_09940"
                     /inference="COORDINATES: protein motif:HMM:NF026954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEU5288715.1"
                     /translation="MDAPMRSHLYTPGHRETMVARVIEGTVATPPDVALLDLEDSVPA
                     GEKENARRVVSAALARPRGVTLRFVRIGRALSDQAEADLAAIVRPGLDGVLVPKVVRA
                     EELAMLDEMLAARERAAGLAPGSVRVIAAIETAAALIDAPRIARAPRVIGLMFGSEDF
                     AAELGLPTRREAEAAELLYARSAVVVAAVAAGVQAFDGIWADFRDEAGLRDDAMRGRR
                     LGFTGRQCIHPDQIAVVNEIFSPGADEVAHARRVVAAYEDGVAKGLGAVALDGEMLDP
                     PIVERARRVLRMTRS"
     gene            complement(1639..>2078)
                     /locus_tag="VFV20_09945"
     CDS             complement(1639..>2078)
                     /locus_tag="VFV20_09945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5288716.1"
                     /translation="NAERILIASESLGDGRWFVGRAAKYSTERVVFGRPIGANQGVQF
                     PIARAHAALAAAGLVRDKAAWLFDHGESCGSEANMAKLLASEAAWAAANATMDAYGGY
                     GFAREFDVERKFRETRLYITAPVNNNLVLAYIAEHVLGLPRSY"
BASE COUNT          295 a          760 c          707 g          316 t
ORIGIN      
        1 ccataacttc gccttcctgg gccccgagtc gaactgggcc gcggaggcgg cactctgcgc
       61 cggcgagcag ggcaagttct tcgagttcca cgaccgcctc ttcgcgtcgc aggccggcga
      121 gaacaagggt gcgttcgcga aggacaagct gaagaggatg ggcacggacc tcgggctcag
      181 cccgtcgttc gccgcctgcg tcgattccgg gaagtacgcc caatccgttc gcgacgagca
      241 gaacgtcggt cagggcaagg gcgtgagcaa gaccccgacg catttcgtga acggcaagaa
      301 gtacgagggc gcgtactcct tcgacgagat gaagaacatc atcgatccgc tcctcgcggg
      361 cggccgctga tcctgagccg tcgcgccgcg ctcgtcttcg cgaccatcgg cacggccgtt
      421 tcggcctacc tcacgtggat ccactacagc ggcgcgttcg cgttctgcgc gggcgtcggc
      481 gattgcgagg tcgtccagac cagccgctac tcgatgctcg gacccatccc cgtcgcgctc
      541 atcggtctcg ccgggatggt gctcatcttc ggcgtctctc tcggccggct cgtgagcgac
      601 gacccgcggc tcgacctcgc gctcttcgcg ctcgcgctca tggcgaccgc ctacgtcgcg
      661 tacctctcct acgttgaggt gttcgtgctg ggcgccatct gtccgtggtg cgtggccgtc
      721 gcggtgtgct ccgtcgcgat cttcgcgctc gccgcgcgcg acgtcatgag cgcgtcatcc
      781 tgagcacacg ccgcgcgcgc tcgacgatgg gcggatcgag catctcgccg tcgagcgcga
      841 cggctccgag gcccttcgcg acgccatcct cgtaggccgc gaccacgcgc cgcgcgtgcg
      901 ccacctcgtc ggcgcccggc gagaagatct cgttcacgac cgcgatctga tccggatgga
      961 tgcactgccg gccggtgaag ccgaggcggc gaccgcgcat ggcgtcgtcc cgcagacccg
     1021 cttcgtcgcg gaagtcggcc cagatgccgt cgaacgcctg cacgcccgcg gccaccgccg
     1081 cgacgacgac cgcggaccgc gcgtagagca gctcggccgc ctcggcctcg cgtcgtgtgg
     1141 gcaggccgag ctcggccgcg aaatcctcgg agccgaacat gaggccgatg acgcgtggcg
     1201 ccctcgcgat gcgcggcgcg tcgatgagcg cggccgccgt ctcgatcgcg gcgatgaccc
     1261 gcaccgaccc cggcgcgagg ccggccgcgc gctcacgcgc ggcgagcatc tcgtcgagca
     1321 tcgcaagctc ctccgcgcgc acgaccttgg gcacgagcac gccgtcgagg cccggccgca
     1381 cgatggcggc gagatcggct tcggcctgat ctgagagcgc ccggccgatg cgcacgaacc
     1441 gcagcgtcac gccgcgtgga cgcgcgagcg cagcgctcac gacgcgcctg gcgttctcct
     1501 tctcgcccgc ggggaccgag tcctcgaggt cgagcagcgc cacgtccggc ggcgtcgcga
     1561 ccgtgccctc gatcacgcgc gcgaccatcg tctcgcggtg tccgggcgtg tacaggtgcg
     1621 agcgcatcgg cgcgtccatc agtacgacct cggcagaccg agcacgtgct ccgcgatgta
     1681 cgcgagcacg aggttgttgt tcacgggtgc ggtgatgtag aggcgggtct cgcggaactt
     1741 gcgctcgacg tcgaactccc tcgcgaaccc gtacccgccg taggcgtcca tcgtcgcgtt
     1801 cgcggcggcc caggcggcct ccgatgcgag gagcttcgcc atgttggcct cgctgccgca
     1861 cgactcgccg tggtcgaaga gccaggcggc cttgtcgcgc accagacccg cggcggccag
     1921 cgccgcatgg gcacgggcga tcgggaactg cacgccctgg ttcgcgccga tgggacgtcc
     1981 gaagaccacg cgctcggtcg agtacttcgc cgcgcgcccg acgaaccagc gaccgtcgcc
     2041 gagcgactcc gacgcgatga ggatccgctc ggcgttcc
//
LOCUS       DASTZR010000448         2516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1147511, whole genome shotgun sequence.
ACCESSION   DASTZR010000448 DASTZR010000000
VERSION     DASTZR010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147511"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VFV20_09950"
     CDS             <1..216
                     /locus_tag="VFV20_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288717.1"
                     /translation="AGLKVSLAHGDTDRAPAEIRHALYELHALTRLHFVDELRTCRNG
                     TASLRAGASRVDAEVISRARRARSTAR"
     gene            complement(179..1039)
                     /locus_tag="VFV20_09955"
     CDS             complement(179..1039)
                     /locus_tag="VFV20_09955"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5288718.1"
                     /translation="MRVLVAFDGSTSAGTALELVRALAWPAGTLVRVAQVIPPPPPFV
                     GSDALADVDARLVEALASSVGRSWSDTVTVDARLVVGDAPADAIVAEARRIGADLIVT
                     GHRGHGAMATVFMGSVARDITEHAPCPVLVARGSTIQRIVVADDGSAGAFRARKIVST
                     WPIFKAKHVDVVSVAHVARPLLAGIAVSVRAEAREAQAESEMEARVAYSRLANEAADG
                     LRLAGLHAAGYVRSGDPAEQICGLARERGADVIVMGTRGRGTIGRVLVGSVARAVLLS
                     APCSVLVVPD"
     gene            complement(1315..1440)
                     /locus_tag="VFV20_09960"
     CDS             complement(1315..1440)
                     /locus_tag="VFV20_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288719.1"
                     /translation="MGRSPFAIDRISSRTRSLWIAGPIDRSSGVNAMFGARVTTH"
     gene            complement(1754..2242)
                     /locus_tag="VFV20_09965"
     CDS             complement(1754..2242)
                     /locus_tag="VFV20_09965"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEU5288720.1"
                     /translation="MGLAKVRHTKRATTARIGAEYRALDRLVRRIGAAGLERPVPGFG
                     ARARIRRERWTGKDALAHIVEWKRQALRALRHEASDPQLRGLSIGRKNRILYDRWHRR
                     PAREVIAYHRAVQREITAALRALPESYFAKRRSPYWPNDLVGHARGHRERHLAALAAE
                     RP"
     gene            complement(2252..>2516)
                     /locus_tag="VFV20_09970"
     CDS             complement(2252..>2516)
                     /locus_tag="VFV20_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEU5288721.1"
                     /translation="IGVEEAGAWKVRAPAVLVRTTVGAGDSMVAGLAIALDEGRGLAE
                     GLRLGTAAGTATCAVPGTQLGSREEIERLLPGISVRSMEALAT"
BASE COUNT          363 a          933 c          865 g          355 t
ORIGIN      
        1 gccggtctca aggtcagcct cgcgcacggc gacaccgatc gggcacccgc ggagatccgt
       61 cacgcgctct acgagctgca cgcgctcacg cgtcttcatt tcgtcgacga gctccggacg
      121 tgccggaatg gcaccgcgag cctccgcgcc ggagcgagcc gcgtcgatgc ggaggtgatc
      181 agtcgggcac gacgagcacg gagcacggcg cgctgagcag tactgcgcgc gcgacgctgc
      241 cgaccaacac gcgcccgatc gtgccgcggc cccgcgtgcc catgacgatg acgtcggcgc
      301 cgcgctcgcg ggcgaggccg cagatctgtt ccgcgggatc gccgcttcgc acgtagcccg
      361 ccgcgtgcag cccggcgagg cgcagcccgt cggcggcctc gttcgcgagg cggctgtacg
      421 cgacgcgcgc ctccatctcg ctctcggcct gtgcctccct ggcctccgca cggaccgaga
      481 ccgcgatgcc ggcgaggagc ggccgtgcga cgtgggccac gctcaccacg tcgacgtgct
      541 tggccttgaa gatcggccac gtggagacga tcttgcgcgc ccggaaggcg cccgccgagc
      601 cgtcgtccgc gaccacgatg cgctggatcg tgctgcctcg cgccaccagc accggacacg
      661 gcgcgtgctc ggtgatgtcg cgtgcgaccg atcccatgaa cacggtcgcc atcgcaccgt
      721 gaccgcgatg gccggtgacg atgagatccg cgccgatccg gcgcgcctcg gcgacgatcg
      781 cgtcggccgg agcatcgccc acgacgagcc tcgcgtccac ggtgaccgtg tctgaccacg
      841 atcggccgac cgacgacgcg agagcctcca ccagccgcgc gtcgacatca gcgagggcgt
      901 ccgatccgac gaacggcggc ggcggaggga tgacctgcgc gacacgcacg agcgtgcccg
      961 ccggccacgc gagcgcccgg acgagctcga gtgccgtccc cgcggatgtg gatccgtcga
     1021 acgcgacgag aacgcgcatc gctccgggcc cctgtccgct cacgccgtcg tcacggggtc
     1081 tatcgcgagg ccgatcgtcg acggttcgac ctcaaccctg gggacgtcgt cctcgtctcc
     1141 ctcccgctcg aaggtctcga ccggtccgaa ctcgagtcgc tcgaccgctg ggcgtcgcac
     1201 atcctgagcg tacgaaggca cttgccgggc acctgtcggg caatcgggca cacggcgatg
     1261 cgggtctcag cagcgctcac gggaccttcg gccgcacgcg gatgccgagc ggccctagtg
     1321 ggtggtgacc cgcgcgccga acatcgcgtt cactcccgaa gacctgtcga tggggcccgc
     1381 gatccagagc gagcgcgttc ttgacgagat ccggtcgatc gcgaacggag agcggcccac
     1441 caagaggatg ttaggcagtg aaccggcgtg ctctcgtcgg caggctcggg agcaaagaga
     1501 agcccgagcg acctgccgac cggagagatg cgttcatgag cgtgtatctc tacggcggag
     1561 ccggtctttg atgcctttcg ttcgaccgtc tctgatgccg ctcccagttt cgcgacgttg
     1621 atgagatctc tgtcgcgatc cctcggcagg cccctcggga tatcccgaga ctgccactcc
     1681 gccgtcgccg ccgagagtgc cgccggtcat gcgccacgca tccgaacgtc gctgtgagtg
     1741 cgcgggaaac gaactacggc cgctcggccg cgagcgccgc gagatggcgc tcgcggtggc
     1801 cgcgggcgtg cccgacgaga tcgttgggcc agtacggcga acggcgcttc gcgaagtagg
     1861 actccggcag tgcccggagc gccgcggtga tctctcgctg caccgctcgg tggtacgcga
     1921 tgacctcgcg cgccggccgg cggtgccagc ggtcgtagag gatccggttc ttccgtccga
     1981 tcgagaggcc gcgcagctgc gggtcggacg cctcatgacg cagcgcgcgc agcgcctgcc
     2041 gcttccactc gacgatgtgc gcgagcgcgt ccttgccggt ccagcgctcg cgccgtatgc
     2101 gcgcccgcgc cccgaagccg gggaccggcc gctcgagccc tgccgcaccg atccgccgaa
     2161 cgaggcgatc gagcgcgcgg tactcggcgc cgatgcgcgc cgtcgttgcg cgcttggtgt
     2221 gccggacctt cgccagcccc atgccgcgat gttaggtcgc gagcgcctcc atggatctga
     2281 cgctgatgcc gggcagcagc cgctcgatct cctcgcggga tccgagctgc gtgcccggca
     2341 ccgcgcaggt cgcggtgccc gccgccgtcc cgagccgaag gccctccgcg agaccacgcc
     2401 cctcgtcgag cgcgatcgcg aggcccgcga ccatcgagtc gccggcgccc accgtcgtac
     2461 gcacgaggac cgccggcgcg cgcaccttcc acgcgccggc ctcctcgacg ccgatg
//
LOCUS       DASTZR010000449         4273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_468562, whole genome shotgun sequence.
ACCESSION   DASTZR010000449 DASTZR010000000
VERSION     DASTZR010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468562"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VFV20_09975"
     CDS             <1..320
                     /locus_tag="VFV20_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5288722.1"
                     /translation="AILDGLSRYSLFWRIVLPLAGPVVASMVITSFMASWNSFAWPVI
                     VLNSPELFTLPIGLNFFKGVYVTRWTLIMAGSMFNTVPVLVVFVLFQRYFIKGIATAG
                     MKD"
     gene            324..1055
                     /locus_tag="VFV20_09980"
                     /pseudo
     CDS             324..1055
                     /locus_tag="VFV20_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245931.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            1199..2611
                     /locus_tag="VFV20_09985"
     CDS             1199..2611
                     /locus_tag="VFV20_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003251312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEU5288723.1"
                     /translation="MSHLSASFSATIRVGLSDDPGSFARVAQAIGDAGGSLGAIDLVR
                     ADGGTKVRDVTVEASDADHLERIVDAVRAVEGVEVEHVSDRTFLLHLGGKLEMRSKTP
                     IRTRDDLSMAYTPGVARICTAIAEDRDAAWNLTIKRNSVAVVSDGTAVLGLGDIGPEA
                     AMPVMEGKAALFKEFGGVDAWPICLSTKDVDQIVSAVAALAPGFGGINLEDISSPRCF
                     EIERRLQEALDIPVFHDDQHGTAIVVLAALLNALGIVGKRLEDVRIVLTGVGAAGIAV
                     TDILLAAGARTIVGCDSVGAIHSGRTDLSSVKQAFCERTNPDSETGSADEILRGADVY
                     LGLSLPGAVSSEGVATMADDAIVFAMANPTPEIAPGDLPPGRVAVVATGRSDYPNQIN
                     NVLAFPGVFRGALDVRASRITEGMKVAAAHALAAVIPHEELGAEYVIPSVFNREVAPA
                     VAAAVARAAQEDGVARRPHG"
     gene            complement(2623..>4273)
                     /locus_tag="VFV20_09990"
     CDS             complement(2623..>4273)
                     /locus_tag="VFV20_09990"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5288724.1"
                     /translation="RTIGASRRQILTSVILESFVVGVLASVVGLFLGLLLAKGLFELF
                     DAVGFTLPNVGLLFETRTVVVALLAGVLVTVLASLRPAIRATRVPPIAAVREGATLPE
                     SRHKRLRTAGALLTIVAGFAALLYGLFGPDLGTTQILIWMGLGALLVFLGVALFSSRL
                     VRPLARLLGWPATKIGGTAGTLARDNSQRNPQRTASTASALMIGLALVTLVATLAAGI
                     VNSFKGAVNDLFTGDYAITAQNNFSPIPIAAAEVVEDVPGVTAVGNVRTGEARIFGST
                     EFATAVDPGMREVISMDWVAGSQDTLATLGADGAFTDDGYAKDHDLTVGSPVTLTFPS
                     GERKTFEIEGIFDPPSGGSPFGVVTISAATWDQEVDRPKNLYSFVKMTGGDTDANQTA
                     LDEALADFPNAKAQNRQSFIDNQISGLSSILNILYVLLALSILVSLFGIVNTLVLTVF
                     ERTREIGMLRAIGMTRRQVRRMIRHESVITSLIGAALGIALGLVLAALLIARVDFIEF
                     SFPTTQVVVFAIAAILVGILAAIFPARRAAKLNPLEALHYE"
BASE COUNT          677 a         1505 c         1480 g          611 t
ORIGIN      
        1 cggccatcct cgacgggctc tcccgctact cgctcttctg gcgcatcgtc ttgccgctcg
       61 ccgggccggt ggtggcgtcg atggtgatca ccagcttcat ggccagctgg aactcgttcg
      121 cgtggccggt gatcgtgctc aactctcccg agctcttcac gctgccgatc ggtctcaact
      181 tcttcaaggg ggtgtacgtg acgcgctgga ccctgatcat ggcaggctcg atgttcaata
      241 ccgtgccggt tctggtcgtc ttcgtgctct tccagcggta cttcatcaag ggcatcgcca
      301 ccgccgggat gaaggattga atcgtggcaa ccgtcgaatt cgagcacatc gtcaagcgtt
      361 acggcaccgc caccccggtc atcagggacc tctgcctgcg catccaggac cgggagttct
      421 tggtgctggt cggtcccagc ggctgcggca agtcgaccgc gctgcggatg atcgccgggc
      481 tggaggacgt ctccggcggc aagctcaaga tcggagaccg ggtggtcaac gacgtccacc
      541 ccaaagagcg cgacatcgcc atggtgttcc agaactacgc gctctacccc cacatgacct
      601 gctacgagaa catggccttc gcgctcaagc tccggaaggt gccgcgcgag gagatccacc
      661 gccgggtcac cgcggccgcc aaggccctgg agatcgagca cctgatcgag cgcaagccca
      721 aagcgctctc cggcgggcag cggcaacgcg tcgccatcgg ccgggccatc gtccgcgagc
      781 cgaaggtctt cttgatggac gagccgctct ccaacctgga cgccaagctc cgggtctcga
      841 tgcgcgcgga gatcaagcgg ctgcaccaga agctcaagac caccttcgtc tacgtgaccc
      901 acgatcagac cgaggccatg accctcggcg accgcatcgc ggtgctcaag ggcggcgatc
      961 tgatgcagtg cgacacgccg cacgagctct acaatcgtcc cgacaacctc ttcgtggcca
     1021 ccttcatcgg ctccccacag atgaacctca tcgaagcgcg gctctcgttc gacggcgagc
     1081 gcgcgctcca cgcgagcgcg ccgccggacg tcgggcagca ctccgccgag gtgctccgcg
     1141 aggcgggctt cgacgacgac gagatcgcta ggctcgccga ggacgggatc gtccgcgtat
     1201 gagccacctc tccgcctcgt tcagcgccac gatccgggtc ggcctctcgg acgacccagg
     1261 ctcgttcgcg cgcgtcgcgc aggcgatcgg cgacgcgggc ggctcgctcg gcgcgatcga
     1321 cctcgtccgc gccgacggcg gcacgaaggt gcgcgacgtc acggtcgagg cgtccgacgc
     1381 cgaccacctc gagcggatcg tcgacgccgt ccgcgcggtc gagggcgtcg aggtcgagca
     1441 cgtctccgac cgcacgttcc tcctccacct cggcgggaag ctcgagatgc ggagcaagac
     1501 gccgatccgc acgcgcgacg acctctcgat ggcgtacacg ccgggcgtcg cccggatctg
     1561 caccgcgatc gccgaggacc gcgacgcagc gtggaacctc acgatcaagc ggaactccgt
     1621 cgccgtcgtc agcgacggca ccgcggtgct cgggctcggc gacatcgggc cggaggcggc
     1681 catgcccgtg atggagggca aggcggcgct cttcaaggag ttcggcggcg tcgacgcgtg
     1741 gccgatctgc ctctcgacga aggacgtcga ccagatcgtc tcggccgtgg ccgcgctcgc
     1801 gcccggcttc ggcggcatca acctcgagga catctcctcg ccgcgctgct tcgagatcga
     1861 gcgccggctc caggaggcgc tcgacatccc ggtcttccac gacgaccagc acggcacggc
     1921 gatcgtcgtg ctcgccgcgc tgctcaacgc gctcggcatc gtcggcaagc ggctcgagga
     1981 cgtgcgcatc gtcctcacgg gcgtcggcgc ggccgggatc gccgtcaccg acatcctcct
     2041 cgccgccggc gcgcggacga tcgtcggctg cgacagcgtc ggcgcgatcc acagcggccg
     2101 caccgacctg agctccgtca agcaagcgtt ctgcgagcgc acgaaccccg acagcgagac
     2161 gggcagcgcc gacgagatcc tccgcggcgc cgacgtgtac ctcgggctct cgctgccggg
     2221 ggcggtcagc tcggagggcg tcgccacgat ggccgacgac gcgatcgtct tcgcgatggc
     2281 gaacccgacg ccggagatcg cgcccggcga cctcccgccc ggccgcgtcg cggtcgtggc
     2341 gacggggcgc tcggactacc ccaaccagat caacaacgtg ctcgccttcc ccggtgtctt
     2401 ccgcggcgcg ctcgacgtgc gcgcctcacg gatcaccgag ggcatgaagg tcgcggccgc
     2461 gcacgcgctc gccgccgtca tcccgcacga ggagctcggg gccgagtacg tgatcccgag
     2521 cgtcttcaac cgcgaggtcg cgccggccgt ggccgcggcc gtcgcgcgcg cggcgcagga
     2581 ggacggcgtc gcgagacggc cgcacggcta gcccccgcgc tcctactcgt agtgcagcgc
     2641 ttccagcggg ttgagcttcg ccgcgcggcg cgcggggaag atcgccgcga ggatcccgac
     2701 gaggatcgcc gcgatcgcga agacgaccac ctgcgtcgtc gggaacgaga actcgatgaa
     2761 gtccacgcgc gcgatcaaca gcgcggcgag gacgagcccg agcgcgatcc cgagcgcggc
     2821 cccgatcagt gacgtgatca cgctctcgtg gcggatcatc cgccgcacct ggcggcgcgt
     2881 catcccgatc gcgcggagca tcccgatctc gcgggtgcgc tcgaagacgg tcagcacgag
     2941 cgtgttcacg atcccgaaga ggctcacgag gatcgagagg gcgagcagca cgtagaggat
     3001 gttgaggatc gagctgagcc cggagatctg gttgtcgatg aagctctggc ggttctgcgc
     3061 cttcgcgttc gggaagtccg cgagcgcctc gtcgagcgcc gtctggttgg cgtcggtgtc
     3121 gccgccggtc atcttcacga acgagtagag gttcttcggc cggtcgacct cctggtccca
     3181 ggtcgcggcc gagatcgtga cgacgccgaa cggcgagccg ccgctcggcg ggtcgaagat
     3241 cccctcgatc tcgaaggtct tccgctcgcc gctcgggaag gtgagcgtca ccggcgagcc
     3301 gacggtcagg tcgtggtcct tcgcgtagcc gtcgtcggtg aacgcgccgt ctgcaccgag
     3361 cgtcgcgagc gtgtcctgcg agcccgcgac ccagtccatc gagatcacct cgcgcatccc
     3421 cgggtcgacc gcggtcgcga actccgtgct gccgaagatg cgcgcctcgc cggtgcgcac
     3481 gttgccgacc gccgtcacgc ccggcacgtc ctcgaccacc tccgcggccg cgatcgggat
     3541 gggcgagaag ttgttctgcg ccgtgatcgc gtagtccccg gtgaagaggt cgttcaccgc
     3601 ccccttgaac gagttgacga ttcccgccgc gagcgtcgcg acgagggtga cgagggcgag
     3661 gccgatcatc agcgccgacg cggtcgaggc ggtgcgctgc gggttgcgct gcgagttgtc
     3721 gcgggcgagc gtgccggccg tccccccgat cttcgtcgcc ggccagccga ggaggcgcgc
     3781 gagcgggcgc acgagccgcg aggagaagag cgcgacgccg aggaagacga gcagcgcgcc
     3841 gaggcccatc cagatcagga tctgggtcgt cccgaggtcg gggccgaaga ggccgtagag
     3901 gagcgcggcg aacccggcca cgatcgtcag cagcgcaccc gcggtgcgga gccgcttgtg
     3961 gcgcgactct ggcagcgtcg cgccctcgcg gacggcggcg atcggcggca cgcgggtcgc
     4021 gcggatcgcg ggccggaggc tcgcgagcac cgtcacgagg acgcccgcga gcagcgcgac
     4081 gacgacggtg cgcgtctcga agagaaggcc gacgttcggc aacgtgaagc cgacggcgtc
     4141 gaacagctcg aagaggccct tcgcgaggag caggccgagg aagagcccca ccacggaggc
     4201 gaggacgccg acgacgaacg actcgaggat cacggaggtg aggatctggc ggcgcgaggc
     4261 gccgatcgtc cgg
//
LOCUS       DASTZR010000450         4439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_828639, whole genome shotgun sequence.
ACCESSION   DASTZR010000450 DASTZR010000000
VERSION     DASTZR010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828639"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..515)
                     /locus_tag="VFV20_09995"
     CDS             complement(132..515)
                     /locus_tag="VFV20_09995"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5288725.1"
                     /translation="MPREGAPFILVIEDDPTIADLLSEIARDAGMHAQVVADPNEVGD
                     GVSPDLIVTDLVAEYAERPEHGLEYLRQLRERFATVPLLLVTARTWPRAALQLPVEAV
                     ISKPFDVDLLSTQMVSLTRHAAPLK"
     gene            578..1156
                     /locus_tag="VFV20_10000"
     CDS             578..1156
                     /locus_tag="VFV20_10000"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEU5288726.1"
                     /translation="MDGLTPRQRDVVEGLSRGLANKQIAAELGISERAVKGHVSDLLR
                     KYGVPSRAGVIAHVLASRPRASLALDPTEFAQYETVPFMVAVTLGPEHRFIFVNATAA
                     AVAGRAAASLVGKAMREAYPDLDQRFEGALDSVYRTGEPWAAPAAPARFPRADGTVAD
                     THLNVMFAPLRDGSGTVVGLLHIGTEVDPEGG"
     gene            1173..1874
                     /locus_tag="VFV20_10005"
     CDS             1173..1874
                     /locus_tag="VFV20_10005"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEU5288727.1"
                     /translation="MLRPDRAIYSIDGGWFRARWHFSFDRYMDPDNMGIGTLRVFNHD
                     TLVPGAVWPMHPHRDIEGITFVVAGEFEHADSLGNGGVLQPGGVQRMRLGSGAEHSER
                     NHSKTSEMQFIQMWIIPRERGLAPDVVQHQYTEADRTDRLLRFLKPEGADGEGLTVAQ
                     DVSMYASRLSPGTSVRHEFGRGRGGYLYLRTGAVSANGERMSAGDAAYVTDAGSLELA
                     ASEASELILVDTPIR"
     gene            complement(1882..3132)
                     /gene="hutI"
                     /locus_tag="VFV20_10010"
     CDS             complement(1882..3132)
                     /gene="hutI"
                     /locus_tag="VFV20_10010"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEU5288728.1"
                     /translation="MSEADLVVRRARVLTLAPLKDERPRIGASAGDVGAIEDGWVAAR
                     DGVVVATGRDDAWERLHLTETATVIDAGGAVVMPGFVDPHTHLCYAGHRWDEFATRRS
                     GGDYLAILERGGGIHSTVRATRDASDAELEALLTRRLAEAAALGTTTIEVKSGYGLEP
                     DAELRMLRVIARASAVTPIDVSATYLALHAMPSQHADDRRAFLQEVVRAVAQVAAEGI
                     AEAVDAFVERGVFETEEVRPLAKAARDAGLALTVHADQLNDVGATEFAARMKARSADH
                     VANASAAGLRALARAAIPAILLPGSAFFVGYTAPDARRFVAADVPIAIATDHNPGTCP
                     LVGMPAAIALAVNLCGLTPQQAIVAATINAAHALRRGDRTGALVKGRRADIVVLATDD
                     ERELAYGLGAPLVGRVFAAGRALG"
     gene            complement(3129..3497)
                     /locus_tag="VFV20_10015"
     CDS             complement(3129..3497)
                     /locus_tag="VFV20_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5288729.1"
                     /translation="MDERRLREFLEAFNRHDLDAIMSFFTEDCVFDMPRGRAPGGAHA
                     EGKAAVRALLATRFTGIPDVHYGDDRHFVAGDRGASEWLLTGTTAAGKRIAVRGCDLF
                     EFEGELVRRKDSYWKIVEEQ"
     gene            complement(3500..4396)
                     /gene="ftcD"
                     /locus_tag="VFV20_10020"
     CDS             complement(3500..4396)
                     /gene="ftcD"
                     /locus_tag="VFV20_10020"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEU5288730.1"
                     /translation="MAALVECVPNISEGRRAEVISQVAAAVAAVPGVRLLDQTSDADH
                     NRSVFTFAGAPDAVLAAAHALADVSLREIDMRTHAGAHPRLGAVDVVPFVPISGISMD
                     ECVALAQRFGREAGERHGVPVYFYAHAATRPERTKLGDIRNKIQYEGLAAALATTHVP
                     DAGPARFNERAGGMVVGARPFLIAFNIELDTTDLALAKRIAKEIRESSGGLPAVQALG
                     FALTDPPRVQVSMNVLDFNTTSLATVWDAVEARARASGVEVLRGELIGLIPLDAALQV
                     TAATLKLDGFDRSRVIEARFLE"
BASE COUNT          644 a         1573 c         1603 g          619 t
ORIGIN      
        1 cacccgggcg agaagatgcc gatcttccgc tgggccttct tcgtcacgcc ttaaacggtt
       61 cgcgaccttc ttcacggacg ccccgctcgt tccgccgaac gagcggggcg ttcggcgtcc
      121 gggaggcccg tctacttcag cggcgcggcg tgccgggtga gggacaccat ctgcgtgctg
      181 agcagatcga cgtcgaaggg cttgctgatg accgcctcga ccggaagctg cagcgccgcg
      241 cgcggccacg tgcgcgccgt cacgaggagg agcggcacgg tcgcgaaacg ctcgcgcagc
      301 tgccgcagat actcgaggcc gtgctccggc cgctccgcgt actcggcgac gaggtccgtg
      361 acgataaggt cgggggacac gccgtctccc acctcattgg ggtcggcgac gacctgagcg
      421 tgcatcccgg cgtcacgagc gatctcgctg aggagatccg cgatcgtcgg gtcgtcctcg
      481 atcacgagga tgaacggtgc gccctctcgc ggcatacggc cattgcaggc gagcgcacgt
      541 agactggcaa gacgtacgaa aggacgggga gcagaggttg gacggcctga cccccaggca
      601 gcgggacgtg gtcgaaggtc tgtcgcgcgg gctcgcgaac aagcagatcg ccgccgaact
      661 cggtatctct gagcgcgcgg tcaaggggca cgtgtccgat cttctcagga agtacggggt
      721 gccgagccgc gccggggtca tcgcgcacgt gctggcgtcg cgccctcgcg cctcgctcgc
      781 cctcgatccg acggagttcg cccagtacga aacggtgccg ttcatggtcg ccgtgaccct
      841 cgggccggaa catcgcttca tcttcgtgaa cgcgaccgcc gcggcggtcg ccggacgggc
      901 ggcggcgtcg ctggtcggca aggcgatgcg cgaggcctat ccggacctgg accagcgttt
      961 cgagggcgcg ctcgattccg tctaccgcac cggcgagccg tgggccgcac cggcagcgcc
     1021 ggcccgcttc ccgcgcgcgg acggcacggt cgcggacacc cacctgaacg tcatgttcgc
     1081 tccactgcgc gacgggagcg ggaccgtcgt cggcctcctt cacatcggga cggaggtcga
     1141 tcctgagggg ggctgacatc cacgccccgc tcgtgctgcg tccggaccgc gcgatctact
     1201 cgatcgacgg aggatggttc cgggcgcggt ggcacttctc gttcgaccgg tacatggacc
     1261 cggacaacat gggcatcggc acgctccgcg tcttcaacca cgacacgctc gtgccgggcg
     1321 cggtctggcc gatgcacccg caccgcgaca tcgagggcat cacgttcgtg gtcgcggggg
     1381 agttcgagca cgccgacagc ctcggcaacg gcggcgtgct gcagcccggc ggcgtccagc
     1441 gcatgcggct cggctccggc gcggagcact cggagcgcaa tcactcgaag acgagcgaga
     1501 tgcagttcat ccagatgtgg atcatcccgc gcgagcgcgg gctcgcaccg gacgtcgtgc
     1561 agcaccagta cacggaggcc gatcgcaccg accggctcct ccggttcctc aagccggagg
     1621 gcgctgacgg cgagggcctc acggtcgcgc aggacgtttc gatgtacgcg tcgcgccttt
     1681 cgcccggcac gagcgtgagg cacgagttcg gtcgcggtcg cggcggctat ctctaccttc
     1741 ggacgggtgc cgtgagcgcg aacggcgagc ggatgagcgc cggcgacgcg gcgtacgtca
     1801 cggacgccgg atcgctcgag ctcgccgcga gcgaggcgag cgagctgatc ctcgtcgaca
     1861 cgccgatccg ctgacgccgc gtcagccgag cgcgcggccg gcggcgaaca cccgcccgac
     1921 gagcggcgcc cccagcccgt acgcgagctc gcgctcgtcg tccgtcgcca gcaccacgat
     1981 gtcggcgcga cggcccttca cgagcgcgcc cgtccgatcg cctcggcgca gcgcgtgcgc
     2041 cgcgttgatc gtcgccgcga cgatggcctg ctgcggagtg agcccgcaca ggttgactgc
     2101 gagcgcgatc gcggcgggca tgccgacgag ggggcaggtc cccgggttgt ggtccgtggc
     2161 gatcgcgatg ggcacgtcgg cggcgacgaa gcggcgcgca tcgggcgccg tgtagccgac
     2221 gaagaaggcg ctccccggca acaggatcgc ggggatcgcc gcgcgcgcca gggcgcgcag
     2281 accggcggcg gacgcgttcg ccacgtggtc ggcggagcgc gccttcatgc gggccgcgaa
     2341 ctccgtcgcg ccgacgtcgt tcagctggtc ggcgtgcacg gtgagggcga ggccggcgtc
     2401 gcgggcggcc ttcgcgagcg gccggacctc ctcggtctcg aacacaccgc gctcgacgaa
     2461 cgcatcgacc gcctcggcga tgccctccgc ggcgacctgg gcgaccgcgc gcaccacctc
     2521 ctgcaggaac gcccgtcgat cgtcggcgtg ctgcgagggc atcgcgtgca gcgcgaggta
     2581 ggtcgcggag acatcgatcg gcgtgacggc gctcgcgcgc gcgatgactc ggagcatgcg
     2641 cagctcggcg tcgggctcga ggccgtagcc gctcttcacc tcgatcgtgg tggtgccgag
     2701 cgccgcggcc tcggcgagcc gccgcgtgag cagcgcctcg agctccgcgt cggacgcgtc
     2761 gcgggtcgcg cgcacagtcg agtggatgcc gccaccgcgc tcgaggatcg cgaggtagtc
     2821 gccgccgctc ctccgcgtcg cgaactcgtc ccagcggtga cccgcgtagc agaggtgcgt
     2881 gtgcggatcc acgaatccgg gcatcaccac cgcgccgccc gcgtcgatga cggtggcggt
     2941 ctcggtcagg tggaggcgct cccacgcgtc atctcggccc gtcgcgacga cgacgccgtc
     3001 gcgggccgcg acccatccgt cctcgatcgc gcccacgtcg cccgccgacg cgccgatgcg
     3061 cggccgctcg tccttcagcg gcgccagcgt gagcacgcgt gcgcgccgaa cgacgagatc
     3121 ggcctcgctc actgctcctc gacgatcttc cagtaggagt ccttccgccg gaccagctca
     3181 ccctcgaact cgaagaggtc acacccgcgc accgcgatgc gcttccccgc ggcggtcgtt
     3241 ccggtgagca gccactcgga ggccccgcgg tcgcccgcga cgaagtggcg gtcgtcgccg
     3301 tagtgcacgt ccgggatacc ggtgaatcgc gtcgcgagca gcgctcgaac ggccgccttg
     3361 ccctcggcgt gcgcgccacc gggcgcccgc ccgcggggca tgtcgaagac gcagtcctcg
     3421 gtgaagaagg acatgatcgc gtcgaggtcg tgccgattga acgcctcgag gaactcgcgg
     3481 agacggcgct cgtccatcgc tactccagga accgcgcttc gatcacgcgc gagcggtcga
     3541 agccgtcgag cttcagcgtg gcggcggtca cctggagggc ggcgtcgagc gggatgaggc
     3601 cgatgagctc gccgcgcagg acctccacgc ccgacgcgcg ggcacgcgcc tcgaccgcat
     3661 cccacaccgt cgcgagcgag gtggtgttga agtcgagcac gttcatcgac acctgcacgc
     3721 gcggcggatc ggtgagcgcg aagccgagcg cctggaccgc ggggagcccg cccgacgact
     3781 cgcggatctc cttggcgatg cgtttcgcca gcgcgagatc ggtcgtgtcg agctcgatgt
     3841 tgaaggcgat gaggaagggg cgcgcgccca ccaccatgcc gcccgcgcgc tcgttgaagc
     3901 gcgccggtcc ggcatccggg acgtgggtcg tggcgagcgc ggccgcgagt ccctcgtact
     3961 ggatcttgtt gcggatgtcg ccgagcttgg tgcgctcggg ccgcgtcgcg gcgtgcgcgt
     4021 agaagtacac cgggacgccg tgccgctccc ccgcctcgcg tccgaagcgc tgcgcgagcg
     4081 cgacgcactc gtccatggag atgccgctga tcgggacgaa cgggaccacg tccaccgcgc
     4141 cgagccgcgg atgcgcgccg gcgtgcgtcc gcatgtcgat ctcgcgcagc gacacgtcgg
     4201 cgagcgcatg ggcggcggcg aggacggcgt ccggagcgcc cgcgaacgtg aacacgctgc
     4261 ggttgtggtc ggcatcgctc gtctgatcga ggaggcgcac gccgggtacc gccgcgacgg
     4321 ccgccgcgac ctgcgagatc acctcggcac gccgaccctc gctgatgttc ggcacgcact
     4381 cgaccaaggc agccacgcga agaatctagt cgagcgacga gcggctttgc cgcgaggag
//
LOCUS       DASTZR010000451         3008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1043227, whole genome shotgun sequence.
ACCESSION   DASTZR010000451 DASTZR010000000
VERSION     DASTZR010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043227"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFV20_10025"
     CDS             complement(<1..306)
                     /locus_tag="VFV20_10025"
                     /inference="COORDINATES: protein motif:HMM:NF013514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU5288731.1"
                     /translation="MPTLRSEKELWDKGLLRVVGVDEVGMAALAGPVVAAACLVTPDT
                     KLIRAVKDSKMLTRAQREELAELIRGRVVRIGIGAASVAEIHRLNIYHASHVAMMRAL
                     "
     gene            complement(353..700)
                     /gene="rplS"
                     /locus_tag="VFV20_10030"
     CDS             complement(353..700)
                     /gene="rplS"
                     /locus_tag="VFV20_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU5288732.1"
                     /translation="MNDVLKTLASEQRKAEVPEIRPGDTVAVSVRVVEGNRERLQKFE
                     GTVLRVRNGGLDEQITVRRIASGVGVERTFLVHSPRVEKIEVTRHGHARRAALYFLRE
                     RVGKAATLREKRR"
     gene            complement(1072..1752)
                     /gene="trmD"
                     /locus_tag="VFV20_10035"
                     /pseudo
     CDS             complement(1072..1752)
                     /gene="trmD"
                     /locus_tag="VFV20_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256255.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
     gene            complement(1749..2282)
                     /gene="rimM"
                     /locus_tag="VFV20_10040"
     CDS             complement(1749..2282)
                     /gene="rimM"
                     /locus_tag="VFV20_10040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEU5288733.1"
                     /translation="MGPRSTSRSIEPDGDALVVGQIRGPHGIRGDVRLDPRTDVKDRF
                     KRGAVFECDGIGPVRITSVRGEPSSPIVHFAGYDSRDAALTLRDRFLRVTRAESRRAT
                     KGAYLWADLVGLAAVTGDGRALGTVRELIRAGGNDVLVVVDDAGHETLHPMLESVVRE
                     VDLAGGRIVLAPQEEMP"
     gene            complement(2251..2478)
                     /locus_tag="VFV20_10045"
     CDS             complement(2251..2478)
                     /locus_tag="VFV20_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEU5288734.1"
                     /translation="MKSLLEHVAKQLVDDPGSVVVTEELDGGFIKLHLRVAEGDVGKV
                     IGRGGRIAKAIRALLKVMATRDGTKVNLEID"
     gene            complement(2517..2786)
                     /gene="rpsP"
                     /locus_tag="VFV20_10050"
     CDS             complement(2517..2786)
                     /gene="rpsP"
                     /locus_tag="VFV20_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU5288735.1"
                     /translation="MAVHIRLRRVGKTKAPTYRVVIADSRAPRNGKFIESIGHYNPRT
                     EPADLVIDAERARHWIKSGARPTATTRSLLVRSGLPESEVPPAVR"
     gene            complement(2850..>3008)
                     /locus_tag="VFV20_10055"
     CDS             complement(2850..>3008)
                     /locus_tag="VFV20_10055"
                     /inference="COORDINATES: protein motif:HMM:NF014972.4"
                     /note="with 4.5S RNA forms a signal recognition particle
                     involved in targeting and integration of inner membrane
                     proteins; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU5288736.1"
                     /translation="SRRRRIAKGSGTTVADVNQLIKQFAEMQKLMKQMGGLAKAGRLP
                     RMPGMPRL"
BASE COUNT          440 a         1075 c         1049 g          444 t
ORIGIN      
        1 gagcgctcgc atcatggcga cgtggctggc gtgatagatg ttcagcctgt ggatctcggc
       61 gaccgacgcg gcgccgatcc cgatgcgcac gacgcgaccg cggatgagct cagcgagctc
      121 ctcgcgctgc gcgcgcgtga gcatcttcga gtccttcacc gcgcggatca gcttggtgtc
      181 gggcgtgacc aggcacgcgg ccgcgacgac gggcccggcg agcgccgcca tcccgacctc
      241 atcgaccccg acgacgcgca gaagcccctt gtcccagagc tctttctcgg aacgaagcgt
      301 cggcacacca gaacaatgcc acggggggcg cgatgtgacg cggggcgcgc tgttagcggc
      361 gcttctcccg cagggtggcg gccttgccga cgcgctcgcg gaggaagtac agggcggcgc
      421 ggcgtgcgtg gccgtggcgc gtgacctcga tcttctcgac gcgcggggag tgcacgagga
      481 acgtgcgctc gacccccaca ccggaggcga tgcggcgcac cgtgatctgc tcgtcgaggc
      541 cgccgttccg cacgcggagg acggtccctt cgaacttctg gaggcgctcg cggttgccct
      601 cgacgacgcg cacgctcacg gcaacggtgt caccaggccg gatctcgggg acctcggcct
      661 tgcgttgctc ggaggcaagc gtcttcaaga cgtcgttcat cggcatggtg atggcactca
      721 cggagtgatc gaaggatacc agaacggcta cggcttcgcc ttcttcagcc ggtcgaggat
      781 cgtgacgtgc gagacgccaa agtgcgccgc gagctgccgc accgtgagcc cggtggcccg
      841 caggcgggcc acctccgcca cgctgggacc gcgctggacg ctccgcgggg cgcgcagccg
      901 tacgcccgcg gccttcaggc gcgcgcgcac cgtcgccgcg gacatcccga agcgggccgc
      961 gatgcgatag gacccgacgc cggaccgata cgccgcgacg aggtcggcgt caccgaccgg
     1021 gctgcgccgc tcggccttgg gaaggcgcgc gcgctccgcc ggcgacagct tcgcgtccgc
     1081 gatgaggtcg ggccggcgct ccgccgtccg ccggatggct tccccacgcc gccacttcac
     1141 gatcgacgcg tggtcgcccg acagcaggac agccgggacg cgcttgccct ggaactccgg
     1201 cggcctggtg tagtgcggcc cctcgagcag cccatcggtg tgcgactcgt gctcgagcga
     1261 ccccgggtcg atcacgccgg gcacgagacg agcgatggcg tcgacgacga tcatggcggc
     1321 gagctcgccc ccggtcagca cgtagtcgcc gaccgacagc tcctcgtcca cgtccgactc
     1381 gatgacgcgc tcgtccacgc cctcgtagtg gccacagaca aggaccacgt gcggcagcgc
     1441 cgcaagacga cgggcggccg cctgatcgaa acggcggccc tgcgcggaga gcagcacgac
     1501 gtgaccctgc gggtcgcgga cggcacggat cgcccgcgag agcggctcgg gcttcatcac
     1561 cattcccgat cccccgccgt acggcgcgtc gtccaccacg tggtgccggt cggtcgtcca
     1621 tgagcggatg tcgtgcaccc gcagctcgat cttcccggcc tgtcgagcgc gcgcgacgat
     1681 gctctccgcg aacgggctct cgaacatctt cgggaacagc gtgagcacgt cgatcgcgag
     1741 cggcccgctc acggcatctc ctcctgcggc gccagcacga tgcggccgcc cgccaggtcc
     1801 acctcgcgca cgaccgactc gagcatgggg tggagcgtct cgtgtcccgc gtcgtcgacg
     1861 accacgagca catcgttccc gcccgcccgg atcagctcgc gcacggtgcc gagcgcgcgt
     1921 ccgtcgccgg tcacggcggc gagaccgacc agatcggccc agaggtacgc ccccttcgtc
     1981 gcacggcgcg actcggcgcg cgtgacgcgc aggaagcggt cgcgcagcgt gagcgcggcg
     2041 tcgcgggagt catagcccgc gaagtgcacg atcggggacg acggctcgcc gcggacactg
     2101 gtgatgcgca ccggaccgat gccgtcgcac tcgaagacgg cgccgcgctt gaagcggtcc
     2161 ttcacgtcgg tgcgcgggtc gaggcgtacg tcgccacgga tgccgtgagg gccgcggatc
     2221 tgaccgacga cgagcgcgtc gccgtcgggc tcaatcgatc tcgaggttga ccttggtccc
     2281 atcgcgcgtg gccatcacct tgagcagcgc gcggatggcc ttcgcgatcc ggccgccgcg
     2341 accgatcacc ttccccacat cgccctcggc gacccggaga tgcagcttta tgaatccgcc
     2401 atccagctcc tcggtgacga cgacggagcc cggatcgtcg acgagctgct tcgcgacgtg
     2461 ctccagcagg ctcttcatac ggccgtcgct cgctgtctgc tcgctcatag ccgagctcat
     2521 cggacggcgg gcgggacctc gctctcgggg aggccgctcc gcaccagcag cgagcgggtc
     2581 gtcgccgtgg gacgcgcgcc gctcttgatc cagtggcggg cgcgctccgc gtcgatgacg
     2641 aggtcggcgg gctcggtgcg cgggttgtag tggccgatgg actcgatgaa cttcccgtta
     2701 cggggcgcgc gcgagtcggc gatgacgacg cggtacgtcg gggccttcgt cttgccgacg
     2761 cggcgcaggc ggatgtggac agccaattct tcggtgatcc tcctggaagt agcagagcga
     2821 cgtggtcgaa ggaccagtct atacgaccgc tacaggcgcg gcatccccgg catgcgcggc
     2881 agccggccgg ccttcgcgag gccgcccatc tgcttcatca gcttctgcat ctcggcgaac
     2941 tgcttgatga gctggttcac gtccgcgacg gtggtgcccg atcccttcgc gatccggcgc
     3001 cgccggga
//
LOCUS       DASTZR010000452         6932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_232842, whole genome shotgun sequence.
ACCESSION   DASTZR010000452 DASTZR010000000
VERSION     DASTZR010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6932
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232842"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VFV20_10060"
     CDS             <1..382
                     /locus_tag="VFV20_10060"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5288737.1"
                     /translation="AQYLLAVPEVIGLTAIAIQSVDPMLRLQLLGLGATSWQSLLILV
                     RESRLLLLTAAMAAFGAVISEIGASLMTGCNLKDDTRILTTLIVLDTGAGDFGGAIAL
                     AIILLAVVFLVNGLTTWVQQRRHA"
     gene            355..1485
                     /locus_tag="VFV20_10065"
     CDS             355..1485
                     /locus_tag="VFV20_10065"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5288738.1"
                     /translation="MGAAAEARLSVLEARDLTVERAGREVVRVEQLALERGEVLAVVG
                     PNGAGKTSLLLALATLVPARGALHANGAPITDQLAYRRRIGIAFQRPLLLDRSVRDNA
                     ALGLTLRGMERASRDRATGAALERLGISHLAARRATALSGGEAQRVSIARALAPSPEI
                     LFLDEPFAGLDAPTRDTLISDLGRILHAEGVATLLVTHDRDEALGLADRIAVLIDGRV
                     RQQGMTDEVFSRPVDEAVAAFLGVENVLPGTIDRATDEMTLVRVGPALVSVTQSPPSG
                     ATRVLVLIAPDDIVIARARGSTSERNVFQARVTNVETIGRRVRLTLDCGFPLVAHVTR
                     QSARELALHVGEQVVASFKATEPHLIAHGRGPQDAGRSERDN"
     gene            1495..2739
                     /gene="glp"
                     /locus_tag="VFV20_10070"
     CDS             1495..2739
                     /gene="glp"
                     /locus_tag="VFV20_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEU5288739.1"
                     /translation="MATSPSAARAASRSLSVEEALDRILARVTPLESVDVALLDGLGA
                     VLAEEAVADRDVPPFRNSAMDGYAVHGAQTAGTRLRVVGSVAAGGTPSGSVGVGEAMR
                     IMTGAPMPDGADTVVRVEDTDNGSQTVTITHDIPAGISVRQAGEDLRRGETILTRGTL
                     LRAADIGVLASIGRERIQVVRRPNVAVLSTGDELVEIGTEPGPGQIRDANRYSIAAAV
                     RATGCAPFEIGIARDDPAELRHALGNAAFGDVALTSGGVSVGDHDHVKPVVDSMGAMD
                     FWSIAIRPGRPLAFGELRTKRGTVPIFGLPGNPVSALLTFELFVRPALLKMSGRTRLH
                     RPRAKARLLDRVEKPGGLRMFARGIYDREAGTVRSTGPQGSGILRSMSLANCLIEIPD
                     TPTSAEPGETVTVILTELPEDH"
     gene            2739..3215
                     /gene="moaC"
                     /locus_tag="VFV20_10075"
     CDS             2739..3215
                     /gene="moaC"
                     /locus_tag="VFV20_10075"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003625539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEU5288740.1"
                     /translation="MPAKRLTHVDAKGAARMVDVGAKPATDRRATARGKLLVSKATLD
                     LVRDGRAPKGDVLGAARIAGIMAAKRTPDLIPLCHPLPLSHASVDLVIEDDGIAIEAT
                     VGTTAPTGVEMEALTAVTVAGLTLYDMLKAVERGARLTEVRLVAKSGGRSGDYTAS"
     gene            3212..3724
                     /locus_tag="VFV20_10080"
     CDS             3212..3724
                     /locus_tag="VFV20_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018502018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEU5288741.1"
                     /translation="MTGIRAGIITVSTRGAAGERPDESGPAMREALTAAGHEVVSAAL
                     VPDDIAKVANAILEAVRAGANVVLTSGGTGLSPNDVTPEATRRVLDREVPGIAEALRA
                     RSLQKTPHGMLSRGVAGAVGGSLVVNLPGSPRAVRESLETLLPVLDHAVELLAGETGE
                     SGHARGRAAG"
     gene            3721..4620
                     /locus_tag="VFV20_10085"
     CDS             3721..4620
                     /locus_tag="VFV20_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEU5288742.1"
                     /translation="MRLAVAPEIAGALAARQPVVALESTVIAHGLPRPTNLETARAVE
                     QEVRAAGATPATIALNAGTAVVGADAALLSRLANEGDVAKVSLRDLAPVLARGGLGAT
                     TVAATVEVAARAGITVMATGGIGGVHRGAERSFDESADLEAIARHPVAVVCAGAKMVL
                     DLTLTLERLETLGVPVVGYRTDELPAFYVRSSGLPVAHRVDDALSAARVVREQIARGA
                     GIVIAVAVPEADAIGRDEAEADVARVVAAAEREGIRGAALTPYLLGALGEATSGRTLR
                     ANVSLLRQNARVAAQIALALAVS"
     gene            complement(4692..5837)
                     /locus_tag="VFV20_10090"
     CDS             complement(4692..5837)
                     /locus_tag="VFV20_10090"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU5288743.1"
                     /translation="MPGSFRIARILGIELRVHLSWVLIFLLVTFSLADQVFPFSYPTW
                     SQQKTVIVAAVTALLFFGSVVLHELAHALVARRFRMSVSSITLFLLGGVASLTKEPPS
                     AKAEFFMAAAGPATSLVIGAIGLGLSQLVTDSLDRAPSLQPIEAVAGYLGYVNIAVAV
                     FNLIPGFPLDGGRVLRSIIWALRSDRMKATRIAARGGQVVAGLFAVWAAYRVITGDPT
                     GLWMGLIAYFLYGAASQTLQQERVVDAVGSARVDQLMTTDFRSVQRGTSIGALIRDVM
                     LPYNLRALPVVDGPRFEGLVTIGDLRKVEQDRWPDTPVDAVMERAAEIPSVTPGDALV
                     VALERFGEDLPLLPVVDAGGALAGLLYRESVVGYVRMREMLGFDARR"
     gene            5948..6637
                     /gene="deoC"
                     /locus_tag="VFV20_10095"
     CDS             5948..6637
                     /gene="deoC"
                     /locus_tag="VFV20_10095"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013467814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEU5288744.1"
                     /translation="MSLPTVRELASLIDHAVLKPTQGERDVVDACTIARREHIATVCV
                     KPFYVPDTARLLKDSGVGVSTVIGFPHGGQAPEVKVATATNALHDGARELDMVINIGA
                     LVDKDYATLEREVGMVVGLAHANEALVKVILECAYLDDAQKAIAAQIAVAAEADYVKT
                     STGFGPEGATVEDVRLLRQVVGDRVGVKAAGGIRTLDDALAMLEAGASRLGTSSTKAI
                     LDDLRQRRGEA"
     gene            6634..>6932
                     /locus_tag="VFV20_10100"
     CDS             6634..>6932
                     /locus_tag="VFV20_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5288745.1"
                     /translation="MTETQSAGRFGDLEVHPDVARAIDELGFMAPTPVQAQAIPILRS
                     GRDLLAQAQTGTGKTAAFAIPIIERVDPTERRPQALIVVPTRELAVQVTREFGSLG"
BASE COUNT         1036 a         2470 c         2516 g          910 t
ORIGIN      
        1 ggcgcagtac ctgctcgcgg tgcctgaggt catcggcctg accgcgatcg cgatccagtc
       61 ggtcgatccg atgctccggc tccagctgct cggcctcggc gcgacgtcgt ggcaatcgct
      121 gctgatcctc gtgcgcgagt cgcggctcct gctgctcacg gccgcgatgg ccgcgttcgg
      181 cgccgtcatc agcgagatcg gcgcgtcgct catgaccggc tgcaacctga aggacgacac
      241 gcgcatcctc accacgctca tcgtcctcga cacgggtgcc ggcgacttcg gcggcgccat
      301 cgcgctcgcg atcatcctgc tcgcggtcgt gttcctggtg aacggactca ccacgtgggt
      361 gcagcagcgg aggcacgcct gagcgtcctc gaagcgcgtg acctcaccgt cgagcgtgcg
      421 ggccgcgagg tcgtgcgtgt ggaacagctc gcgctcgagc gcggcgaggt gctggccgtc
      481 gtcgggccga acggggccgg caagaccagc ctgctcctcg ccctcgcaac tctcgtcccc
      541 gcacgcggcg cgctccacgc gaacggggcg cccatcacgg atcagctcgc gtaccggcgc
      601 cgcatcggca tcgccttcca gcgaccgctg ctgctcgacc gcagcgtgcg cgacaacgcc
      661 gcgctcggcc tcacgctccg tggcatggag cgggcctcac gcgatcgcgc caccggcgcg
      721 gccctcgagc ggctgggcat ttcgcatctc gccgcacggc gtgcgaccgc gctctccggc
      781 ggagaagcgc aacgcgtttc gatcgcccgc gcgctggcgc cgtcgcccga gatcctcttc
      841 ctcgacgagc ccttcgccgg gctcgatgcc cccacccgcg acacgctcat ctcggacctc
      901 ggccgcatcc ttcacgccga gggcgtcgcg acgctcctcg tcacgcacga tcgcgatgag
      961 gcgctcgggc tcgccgatcg catcgcggtg ctcatcgacg gccgggtgcg ccagcagggc
     1021 atgacggatg aggtcttcag ccgcccggtc gacgaggcgg tcgcggcgtt cctcggcgtc
     1081 gagaacgtcc tgccggggac gatcgaccgc gcgacggacg agatgacgct ggtgcgcgtc
     1141 ggacccgcgc tcgtctccgt gacgcagtcg ccgccgtccg gagcgacccg cgtgctcgtg
     1201 ctcatcgcgc ccgacgacat cgtgatcgcg cgcgcacgcg ggagcacgag cgagcgcaac
     1261 gtcttccaag cgcgtgtgac gaacgtggag acgatcgggc gacgtgtccg gctgacgctg
     1321 gactgcggct tcccgctggt cgcgcacgtg acgcgccaat cggcgcgcga gctcgcactc
     1381 cacgtcggcg agcaggtggt cgcgtcgttc aaggcgaccg agccgcatct gatcgcgcac
     1441 ggccgcgggc cgcaggacgc gggccgaagc gagcgcgaca actagactcc acccatggcc
     1501 acgagcccga gcgcggcacg cgcggcgtct agatcgctca gtgtggaaga ggcgctcgac
     1561 cgcatcctcg cacgcgtgac gccgctcgag agcgtcgacg tggcgctgct cgacgggctg
     1621 ggcgcggtcc tcgcggagga ggccgtggcc gatcgggacg tgccgccgtt ccgcaactcg
     1681 gcgatggatg ggtacgccgt ccatggcgct cagacggccg gcacccggct ccgcgtcgtc
     1741 ggttccgttg ccgccggtgg cacgccgagc ggctcggtcg gcgtcggcga ggcgatgcgg
     1801 atcatgaccg gcgcgccgat gcccgacggc gccgacacgg tcgtgcgcgt cgaggacacc
     1861 gacaacgggt cgcagaccgt gaccatcacg cacgacatcc cggccggcat ctccgtgcgc
     1921 caggcgggcg aggacctgcg ccgtggagag acgatcctca cgcgcggcac gctcctgcgc
     1981 gccgcggaca tcggcgtcct ggcctcgatc ggccgcgagc gcatccaggt ggtgcggcgg
     2041 ccgaacgtgg ccgtgctgtc gacgggcgac gagctcgtcg agatcggcac ggagcccgga
     2101 ccggggcaga tccgcgacgc caatcgttac tcgatcgccg cggccgtgcg cgcgaccggt
     2161 tgcgccccgt tcgagatcgg catcgcgcgc gacgatcccg cggagctgcg tcacgcgctc
     2221 ggcaacgccg cattcggcga cgtcgcgctc accagcggcg gcgtgtccgt gggcgaccac
     2281 gatcacgtga agccggtcgt cgattcgatg ggcgcgatgg acttctggtc gatcgccatc
     2341 cgtcccggac ggccgctcgc cttcggcgag ctccgtacga agcggggcac cgtgccgatc
     2401 ttcggcctgc cggggaatcc cgtctccgcg ctcctcacct tcgagctctt cgtgcggccg
     2461 gccctgctga agatgtccgg acggaccagg ctgcatcggc cgcgcgcgaa ggcgcgcctg
     2521 ctggaccgcg tggagaagcc cggcggactc cgcatgttcg cgcgcggcat ttacgaccgc
     2581 gaggcgggga ccgtgcgttc gacggggccg cagggctcgg gcatcctgcg ctcgatgtcg
     2641 ctcgcgaact gcctcatcga gatcccggac acgccgacgt cggccgagcc cggcgagacc
     2701 gtgaccgtca tcctcaccga gctccccgag gaccactgat gccggcgaag aggctgacgc
     2761 atgtcgatgc caagggagcc gcgcgcatgg tcgacgtcgg cgcgaagccg gcgaccgatc
     2821 gccgagcgac ggcacgcggc aagctgctcg tctcgaaggc cacgctcgac ctcgtccgcg
     2881 acggccgcgc tccaaagggc gacgtgctgg gggccgcgcg catcgcgggc atcatggccg
     2941 cgaagcggac ccccgatctc atcccgctgt gccatccgct gcccctctcg cacgcgagcg
     3001 tggacctcgt gatcgaggac gacggcatcg cgatcgaggc cacggtcggg acgaccgcac
     3061 cgaccggcgt cgagatggag gcgctgaccg cggtgaccgt cgccggcctc acgctgtacg
     3121 acatgctgaa ggccgtcgag cgcggcgccc ggctcaccga ggtgcggctc gtcgcgaaga
     3181 gcggcggccg cagcggcgac tacaccgcat cgtgaccggc atccgcgccg ggatcatcac
     3241 cgtcagcacg cgcggcgccg ccggcgagcg gcccgatgag agcggaccgg cgatgcgcga
     3301 ggcgctcacc gccgcaggac acgaggtcgt gagcgcggcg ctcgtgcccg acgacatcgc
     3361 caaggtcgcg aacgccatcc tcgaggccgt gcgcgcgggc gcgaacgtcg tgctcacgag
     3421 cggcggcacc ggtctctccc ccaacgacgt cacgccggag gcgacgcgcc gcgtcctcga
     3481 ccgtgaggtg cccggcatcg cggaagcgct gcgcgcgcgg tcgctccaaa agacgccgca
     3541 cgggatgctc tcacgcggcg tcgcgggcgc ggtcggcggg agtctcgtcg tcaacctacc
     3601 cgggtcgccc cgcgcggtgc gcgagtcgct cgagacgctc ctacccgtgc tggaccacgc
     3661 ggtcgagctc cttgccggcg agacgggcga gagcgggcac gcacggggcc gcgcggccgg
     3721 atgaggctcg cggtcgcgcc cgagatcgcg ggcgcgctcg cggcgcggca gccggtcgtc
     3781 gcgctcgaat ccacggtcat cgcccacggt ctcccgcgtc cgacgaatct tgagactgcc
     3841 cgcgccgtcg aacaggaggt ccgcgccgcc ggagctaccc cggccacgat cgcgctgaac
     3901 gcgggtaccg cggtcgtcgg cgcggacgcc gcgctgctct cgcggctcgc gaacgagggc
     3961 gacgtcgcga aggtgagcct gcgcgatctc gctccggtgc tcgcccgcgg cgggctcggc
     4021 gcgaccaccg tggcggcgac cgtcgaggtc gcggcgcgcg ccggcatcac cgtgatggcc
     4081 acgggtggta tcggcggcgt gcatcgcgga gcggagcgct cgttcgacga gtccgcggat
     4141 ctcgaggcca tcgcgcgcca tccggtggcc gtcgtctgcg ccggagccaa gatggtgctc
     4201 gacctcacgt tgacgctgga acgcctcgag acgctcggcg tgccggtcgt cggctaccgc
     4261 accgacgagc tccccgcttt ctacgtgcgt tccagcggcc tgcccgtggc acaccgcgtc
     4321 gacgacgcgc tctccgcggc gcgtgtcgtc cgcgagcaga tcgcgcgcgg cgcggggatc
     4381 gtgatcgcgg tggccgtccc cgaagcggac gccatcggcc gtgacgaggc cgaggcggac
     4441 gtcgcgcgcg tggtcgccgc cgcggagcgc gagggcatcc gcggcgcggc gttgacgccc
     4501 tacctgctcg gcgcgctcgg agaggcgacg agcgggcgga cccttcgcgc caacgtctcg
     4561 ctgctccggc agaacgcgcg cgtcgccgcg cagatcgcgc tcgcgctggc ggtcagctaa
     4621 ggacttccgt caccgtcgcg gagcgcaggt cgcggatcgg cgacgccagc aggtagacga
     4681 aggcgacgat gtcagcggcg ggcgtcgaag ccgagcatct cccgcatgcg cacgtagccg
     4741 acgacggact cccggtacag cagacccgcc agcgcgccgc ccgcatccac caccggaagg
     4801 agcggcaggt cctcgccgaa acgctcgagg gcgacgacga gcgcgtcgcc gggggtcacc
     4861 gaggggatct ccgccgcccg ctccatgacg gcgtcgacgg gggtgtcggg ccaccggtcc
     4921 tgttccacct tgcgcaggtc gccgatcgtg acgagaccct cgaaacgcgg gccatcgacg
     4981 accggcagcg cgcgcaagtt gtacggaagc atgacgtcgc ggatgagcgc gccgatggac
     5041 gtgccgcgct gtaccgagcg gaagtccgtg gtcatgagct ggtcgacgcg cgcgctgccc
     5101 acggcgtcga cgacgcgctc ctgctgcagc gtctgcgacg ccgcaccgta gaggaagtac
     5161 gcgatgaggc ccatccacag accggtcggg tcgccggtga tcacccggta cgcggcccag
     5221 accgcgaaga ggccggcgac gacctgaccg ccgcgcgcgg cgatgcgcgt ggccttcatc
     5281 cgatcgctgc gaagcgccca gatgatcgac cgcagcacgc ggccgccgtc gaggggaaaa
     5341 ccggggatga ggttgaagac ggctaccgcg atgttcacgt agccgaggta gccggcgacg
     5401 gcctcgatgg gctggaggct cggtgcgcga tcgagtgaat cggtgacgag ctgcgacagc
     5461 ccgaggccga tcgcgccgat cacgaggctc gtggcggggc cggcggcggc catgaagaac
     5521 tcggccttgg ccgacggcgg ctccttggtg aggctcgcga cgccgccgag gaggaacagc
     5581 gtgatcgacg acaccgacat gcggaagcgt cgcgcgacca gcgcgtgcgc gagctcgtgc
     5641 agcaccacgg aaccgaagaa gagcagcgcg gtgacggcgg cgacgatgac ggtcttctgc
     5701 tgcgaccagg tcggatacga gaacgggaag acctgatcgg cgagtgagaa cgtcacgagg
     5761 aggaagatca ggacccagga gagatggacg cgcagctcga tcccaaggat gcgtgcgata
     5821 cggaacgagc caggcacgaa gcgaggatag cggcgatact gcatgtgaga cgtatggccg
     5881 tacccggggt ggcaggggcg gagcccctgc gagagacagg tcctttgcca atggcggcga
     5941 agccggcatg agcttgccga ccgtacgcga gctggcatcg ctcatcgacc acgcggtcct
     6001 caagccgacc cagggcgagc gcgacgtcgt cgacgcgtgc accatcgcgc gcagagagca
     6061 catcgcgacg gtgtgcgtga agcccttcta cgtccccgac acggcgcggc tgctcaagga
     6121 ctcgggcgtc ggggtgtcca cggtcatcgg cttcccgcac ggcgggcagg cgccggaggt
     6181 gaaggtcgcg accgcgacga acgcgctcca cgacggagcg cgcgagctcg acatggtcat
     6241 caacatcggc gcgctcgtcg acaaggacta cgccacgctc gagcgcgagg tcggcatggt
     6301 ggtcgggctc gcacacgcga acgaggctct cgtgaaggtc atcctcgagt gcgcgtacct
     6361 cgacgacgcg cagaaggcga tcgccgcgca gatcgccgtc gcggcggagg ccgactacgt
     6421 gaagacgtcc accggcttcg gcccggaggg cgcgacggtc gaggacgttc ggctgctccg
     6481 acaggtcgtc ggcgacaggg tcggcgtgaa ggccgcgggc gggatccgca cgctcgacga
     6541 cgcgctcgcg atgctcgagg ccggcgcgag ccgccttggg acgtcgtcga cgaaggcgat
     6601 cctcgacgac cttcgacaac ggcgcggcga ggcgtgaccg agacgcagtc ggcggggcgc
     6661 ttcggcgatc tcgaggtcca ccccgatgtc gcgcgcgcga tcgacgagct gggattcatg
     6721 gcgccgacgc cggtgcaggc gcaggccatc ccgatcctgc ggtccggccg cgatctcctc
     6781 gcgcaggcgc agaccggcac gggcaagacc gcagcgttcg ccatccccat catcgagcgc
     6841 gtcgatccga ccgagaggcg cccgcaggcg ctcatcgtcg ttcccacgcg cgagctggcg
     6901 gtgcaggtga cgcgcgagtt cgggtcgctc gg
//
LOCUS       DASTZR010000453         2533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_201672, whole genome shotgun sequence.
ACCESSION   DASTZR010000453 DASTZR010000000
VERSION     DASTZR010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201672"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VFV20_10105"
     CDS             <1..545
                     /locus_tag="VFV20_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288746.1"
                     /translation="IRVWRLGHSVPSGEMDQLFALDAAAPPSPAAPTEDRAPSAAPSS
                     ISTRSAWLIGVTTLLLAGVLVADGIVAGFLPGGRDWGLSGDSSGWLVYLFAVVALTVA
                     TRAVHGSRIALRLLTATALLFFIERAFSPFVLRVLDPIALALHLLAAFTALAVALASA
                     SAIRGARPRSDRDVASLEAA"
     gene            572..1534
                     /locus_tag="VFV20_10110"
     CDS             572..1534
                     /locus_tag="VFV20_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU5288747.1"
                     /translation="MPTVQEATARLAEQVARVVVGKRTATEQLFAAILVEGHVLLDDV
                     PGVGKTTLARAVARSFSLSFHRIQCTPDLVPSDITGVSIYDQRTERFEFRPGPVMANI
                     VLVDEINRATPRAQSSLLEAMQERQVTIDETTHRLPEPFCVIATQNPIELEGTFPLPE
                     AQLDRFLFLVKLGYPDAGEEDEILRVHGPATVGTDTLDPIYDASVIAQLRDEVRAVKV
                     GDAVRRYIADITRATRKADGVELGASPRAAIALYRAAQALAAMRGRTYVLPDDVKAQA
                     GSVLRHRIFLGADAQMRGRTGAAVVSEVLARTPVPAEVDAATAG"
     gene            1534..>2533
                     /locus_tag="VFV20_10115"
     CDS             1534..>2533
                     /locus_tag="VFV20_10115"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEU5288748.1"
                     /translation="MVRSGRFEWPLVLLFVLIAAISRSPALIVLAAGGLVLGVMVWLT
                     SRLALVALDATVELSADRVVAGEGLTAVVRVVNRKPMPLPWLDVRLLLPDGIVPDPMP
                     PGIAHGFVDAGFSPRGHEKLTVRFPLLSTQRGAYAFGPVRLRASDWLGFFSVERTLPI
                     ALSLVAYPAPLATRDRHLPSLRPLAETAVRRGLVPDPLRFRGVREHRAGDPRKEIHWK
                     ASARLRTLQTKLYEPATSLDAVFLVNVASYEQYWIQADPEAVEIVVSAAADLVRQAAR
                     AGRQVGLVTNGIDNLTHERPRSALGRGPHSLTRALEILARLGPYAANSPETVFLGER"
BASE COUNT          311 a          943 c          870 g          409 t
ORIGIN      
        1 cgatccgggt gtggcggctc ggacactcgg tgccgtccgg cgagatggac cagctcttcg
       61 cgctcgacgc cgccgccccg ccatcgccgg cagccccgac ggaggaccgc gcaccgtccg
      121 ccgcgccgtc gtcgatctcg acgcgctcgg cctggctcat cggcgtcacg acgctgctcc
      181 tggccggcgt gctcgtggcg gacggcatcg tcgccggatt cctgccgggc ggacgtgatt
      241 gggggctctc cggagactcc agcggctggc tcgtgtacct gttcgcggtc gtcgcgctga
      301 ccgttgcgac gcgcgcggtg catggcagcc gcatcgcgct ccgcctgctg accgccaccg
      361 cgctcctctt cttcatcgag cgtgcgttca gtccattcgt cctgcgcgtg ttggatccga
      421 tcgcgctcgc actgcacctg ctcgccgcgt tcaccgccct cgccgtcgcg ctcgcgtccg
      481 cgagcgcgat ccgcggcgcg cggccccggt cggatcgtga cgtcgcttcg ctcgaagccg
      541 cctaacacgt cgctcctcta gtctcagacc gatgccgacc gtccaggaag ccacggcgcg
      601 actcgccgag caggtcgcgc gcgtggtcgt cggaaagcgc actgcgaccg agcagctctt
      661 cgccgcgatc ctcgtcgagg gccacgtgct gctcgacgac gtccccggag tggggaagac
      721 cacgctggct cgggccgtgg cgcggtcgtt ctcgctgtcg ttccaccgca tccagtgcac
      781 gccggacctg gtgccgtcgg acatcaccgg cgtgtcgatc tacgaccagc ggacggagcg
      841 cttcgagttc cgtcccggcc cggtgatggc gaacatcgtg ctcgtcgacg agatcaaccg
      901 tgcgaccccg cgcgcccagt cgagcctgct cgaggcgatg caggagcgcc aggtcacgat
      961 cgacgagacg acgcaccggc tgccggagcc gttctgcgtc atcgcgacgc agaacccgat
     1021 cgagctcgag gggacgttcc ccctgccgga ggcgcagctc gaccgcttcc tcttcctcgt
     1081 gaagctcggc tacccggacg ccggcgagga ggacgagatc ctgcgcgtcc acggcccggc
     1141 gaccgtcggc acggacacgc tcgatccgat ctacgacgcg agcgtcatcg cgcagctccg
     1201 tgacgaggtg cgtgccgtca aggtcggcga cgcggtgcgg aggtacatcg cggacatcac
     1261 gcgcgcgaca cgcaaggcgg acggcgtcga gctcggcgcg agcccgcgcg cggcgatcgc
     1321 gctgtatcgg gcggcgcagg cgctcgccgc gatgcgcggc cgcacgtacg tgctcccaga
     1381 tgacgtgaag gcgcaggccg gttcggtgct gcgtcaccgg atcttcctcg gcgccgatgc
     1441 gcagatgcgc ggtcgcaccg gcgccgccgt cgtgagcgag gtcctcgcgc ggacgccggt
     1501 gccggcggaa gtggacgcgg ccacggccgg ctgatggtcc gatccggccg cttcgagtgg
     1561 ccgctcgtct tgctcttcgt gctcatcgcc gcgatctcgc ggtctccggc gctcatcgtc
     1621 ctcgccgctg gcggactcgt cctgggtgtc atggtctggc tcacgtcgcg gctcgcgctc
     1681 gtggcgctcg acgcaacggt cgagctcagc gccgatcgcg tcgtcgcggg cgaggggctc
     1741 accgcggtcg tgcgcgtcgt gaaccgcaag ccgatgccct tgccctggct cgacgtgcgg
     1801 ctcctccttc ccgatgggat cgtgcccgac ccgatgccgc ccggtatcgc gcatggcttc
     1861 gtcgatgccg gcttctcgcc acgcggtcac gagaagctga cggtgcgctt tccgctcctc
     1921 tcgacgcagc gcggcgcgta cgccttcggg ccggtgcgcc tgcgtgcgag cgactggctg
     1981 gggttcttct ccgtcgagcg gacgctcccg atcgcgctct cgctggtcgc gtatccggcg
     2041 ccgctcgcga cccgcgaccg gcatctcccg agcctgcggc cgctcgctga gacggccgtg
     2101 cgccgggggc tcgtgcccga tccgctgcga ttccgcggcg tgcgcgagca tcgcgccggc
     2161 gatccgcgca aggagatcca ctggaaggcg tccgcgcgcc tccgcacgct gcagacgaag
     2221 ctgtacgagc ccgccacgag cctcgacgcg gtcttcctcg tgaacgtcgc gtcgtacgag
     2281 cagtactgga tccaggccga tcccgaggcg gtggagatcg tcgtctctgc ggccgcggac
     2341 ctcgtgcgcc aagccgcgcg cgccgggagg caggtcggcc tcgtgaccaa cggcatcgac
     2401 aacctgaccc acgagcgccc tcgttccgct ctgggccgtg gtccccattc gctcacgcga
     2461 gcgctcgaga tactggcgcg tctcggcccg tacgccgcga actcaccgga gaccgtcttc
     2521 ctcggcgagc gcg
//
LOCUS       DASTZR010000454         2461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_181377, whole genome shotgun sequence.
ACCESSION   DASTZR010000454 DASTZR010000000
VERSION     DASTZR010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181377"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..107
                     /locus_tag="VFV20_10120"
     CDS             9..107
                     /locus_tag="VFV20_10120"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEU5288749.1"
                     /translation="MTDASAFPSASGVNPMLSIMAIARRTAQRMLV"
     gene            165..1010
                     /locus_tag="VFV20_10125"
     CDS             165..1010
                     /locus_tag="VFV20_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016828037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEU5288750.1"
                     /translation="MTFRRDAPLDPSQVEDRRGAGGLLIGGGGIGIVILIASLLLGVD
                     PTAMLEAIPTDTAVQNGPTATECKTGSQANERDDCRIVGFVDSVQKYWTDEFARTGKQ
                     YAPAKTVLFSGYAQSACGTAQTAMGPFYCPEDGKVYMDITFFQDLRSKLGAQAGPFAQ
                     GYVVAHEYGHHVQHLIGGLNTSTRTGAESQSVRTELQADCFAGVWAKNASDTGYLETP
                     TQAQIADALDAAAAVGDDRIQQQTQGRVAPDSFTHGSSAQRQKWFTTGYQTGDPARCD
                     TSGAL"
     gene            1067..2134
                     /locus_tag="VFV20_10130"
     CDS             1067..2134
                     /locus_tag="VFV20_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288751.1"
                     /translation="MGLLSNESVSMPASGFADGVEFEETFPEPEATQARPHLSVVRDE
                     AEPVATAEPAARDAVADDPLERRLREAEAMVKDTVERMRLAEEQRLATWARERREEEE
                     RRLAAWIDERRATVERSLDQRSSREDDLAARLEAMLVEWQVRFEQRLEQRRADDERMA
                     ERRRQNDEERLRAWRRELEQALEARFAEPHRQSPAPARAEPRSPFHDAIERAASARDV
                     GRLLRDALADMARTSALALSIHHPQRDEVAYRYRVASEDELGALLRGESLDDGPESPA
                     AHMDGWVRAHRAVRIGTRNAVVHTTQCAVRAGDATIGVLTLQSEGDAIADDVLARLSD
                     LVLGAAPRLAALRDRGNYRGA"
     gene            complement(2141..>2461)
                     /locus_tag="VFV20_10135"
     CDS             complement(2141..>2461)
                     /locus_tag="VFV20_10135"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEU5288752.1"
                     /translation="GGPAVTFWSSIVLGGIATYLTRAVPLFVTPRGTAPAALRRYLDA
                     LPIAVIAALAGAGIAFPDGQPTGGAEIAAAGVAFALAAWRRNLLLAMIAGVVIVALLR
                     GIGL"
BASE COUNT          372 a          900 c          877 g          312 t
ORIGIN      
        1 gcctcttcgt gaccgacgcg agcgcgttcc cgagcgcgtc cggcgtgaac cccatgctct
       61 cgatcatggc catcgcgcgc cgcaccgcgc agcgcatgct cgtgtgatcg ctcggctcgg
      121 gcctgcacgg cgccgctggc cccggtcttg cgtgtaccag cggtgtgaca ttccgtaggg
      181 acgcgccgct cgatccctct caggtcgagg accgccgcgg cgccgggggc ctgctcatcg
      241 gcggcggcgg catcggcatc gtgatcctca tcgcgtcgct gctgctcggc gtcgatccca
      301 ccgccatgct cgaggcgatc cccaccgaca ccgccgtcca gaacgggccg accgcgacgg
      361 agtgcaagac cggttctcag gcgaatgagc gggacgactg ccggatcgtc ggcttcgtgg
      421 acagcgtgca gaaatactgg accgacgagt tcgcgcgcac cggcaagcag tacgcaccgg
      481 cgaagaccgt gctgttcagc ggatacgcgc agtccgcgtg cgggacggcg cagaccgcga
      541 tgggcccctt ctactgcccg gaggacggga aggtgtacat ggacatcacc ttcttccagg
      601 acctgcgctc caagctcggt gcgcaggcgg ggccgttcgc gcagggctac gtcgtcgcgc
      661 acgagtacgg tcaccacgtg cagcacctca tcggcgggct gaatacctcg acgcgcaccg
      721 gtgcggagag ccaatcggtg cgcaccgagc tgcaggcgga ctgctttgcg ggtgtctggg
      781 cgaagaacgc cagcgacacc ggctatctcg aaacgccgac gcaggcacag atcgccgacg
      841 ccctcgacgc cgccgccgcg gtcggcgacg atcgcatcca gcagcagacg cagggtcgcg
      901 tcgccccgga ctcctttacc cacggctcgt cggcgcagcg tcagaagtgg ttcacgaccg
      961 ggtatcaaac gggtgacccg gcacgctgcg acacctcggg agcgctctag gaggttcccc
     1021 cgttgtcggg atgtccgacg acggtgcggt cgcgagactc ttcggcgtgg gactcctctc
     1081 gaacgaatcc gtgtcgatgc ccgcgtccgg cttcgcggac ggcgtcgaat tcgaggagac
     1141 gttccccgag ccggaggcca cgcaggcgcg tccccatctc agcgtggtcc gcgacgaggc
     1201 cgagccggtc gcgaccgcgg agccggccgc gcgcgacgcg gtcgccgatg acccgctcga
     1261 gcgccgcctg cgcgaggccg aggcgatggt caaggacacc gtggagcgca tgcgcctcgc
     1321 cgaagagcag cggctcgcga cgtgggcacg cgaacgtcgc gaagaagagg agcggcgcct
     1381 cgcggcatgg atcgacgaac ggcgcgcgac cgtggagcgg tcgctcgacc agcgatcgtc
     1441 gcgcgaggat gacctcgcgg cgcgcctcga ggcgatgctc gtcgagtggc aggtccgctt
     1501 cgagcagcgg ctcgaacagc gccgcgccga cgacgagcgc atggccgagc gccgccgcca
     1561 gaacgacgag gagcgccttc gcgcctggcg ccgcgagctg gagcaggcgc tcgaggcgcg
     1621 attcgccgag ccgcaccggc agtcgcccgc accggcacgc gccgagccgc gctcgccatt
     1681 ccacgacgcg atcgagcgcg cggcgtccgc gcgtgacgtc gggcgactgc tccgcgacgc
     1741 gctcgccgac atggcccgca cgtccgccct cgcgctctcg atccatcatc cgcagcgcga
     1801 cgaggtcgcg taccggtatc gcgtcgcgtc ggaggacgag ctcggcgcgc tgctgcgcgg
     1861 cgagtccctc gacgacggcc cggagagccc cgccgctcac atggacggat gggtccgggc
     1921 gcatcgtgcg gtgcgcatcg gcacgcgcaa cgcggtcgtc cacacgacgc agtgcgccgt
     1981 ccgcgcgggc gacgcgacga tcggcgtcct cacgctgcag agcgagggcg acgcgatagc
     2041 ggacgacgtg ctcgcgcgcc tctcggacct cgtgctcggt gcggcgccgc gcctcgcggc
     2101 actccgggac cgcggcaact accgcggtgc gtaggtcgcg ctagagcccg atccctcgca
     2161 ggagcgcgac gatcacgacc ccggcgatca tcgccagcag cagattgcgc cgccacgccg
     2221 cgagcgcgaa cgcgacgccc gccgcggcga tctccgcgcc gccggtcggc tgcccatcgg
     2281 ggaaggcgat gcccgcgccc gcgagcgcgg cgatgaccgc gatcggcagc gcgtcgaggt
     2341 agcgccgcag cgccgccggc gccgtgccgc gcggcgtcac gaagagcggc acggcccgcg
     2401 tgaggtacgt ggcgatgccg ccgagcacga tcgagctcca gaacgtcacc gccggtcctc
     2461 c
//
LOCUS       DASTZR010000455         3157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_384569, whole genome shotgun sequence.
ACCESSION   DASTZR010000455 DASTZR010000000
VERSION     DASTZR010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384569"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VFV20_10140"
     CDS             complement(<1..773)
                     /locus_tag="VFV20_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288753.1"
                     /translation="MKLIGRRTAVIAVTTVFGLSMLGSAAFAALAPAATNDTVSVLAP
                     EPGANTLTEAPRGNKLKDVLDALVSKGVITQQQEDAILAALKEKSHDRDREELLRRIF
                     AGLFQQSATYLGMQPADLKAKLPGTSLGAIANATAGKSRDGLVTALVNAVNDAIAKAV
                     ADGKITQEQADKAKASVPERVAKFVDHTWPTPKPRLPSVRAFIGDVMSDARAYLGIAQ
                     KDLMTQLRAGKSLGEIADATPGKSKSGLVSTLMNDANAK"
     gene            910..2328
                     /locus_tag="VFV20_10145"
     CDS             910..2328
                     /locus_tag="VFV20_10145"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HEU5288754.1"
                     /translation="MRIVFAAAEAAPYAKVGGLADVAGSLPQALAALGHDVTLYLPLH
                     ATIDRARFGIEGSGPTRSVAYGKSAARVAYPTIQHDGVRVVFVDSARRLARDKVYGAK
                     DDNKRYAFFCRAVFEDVREAKPDVVHAHDWHTALLVPLVRRARSLARTATIFTIHNLA
                     YQGRTSAEILKLIGLPRARLAIEDPGECNPMARGIAYADIVSTVSQQYAKEILTREFG
                     ERLDGLLRERRQHLAGIVNGIDTKAFDPATDANIAARYASGDPAAKAKDKEALQAEGG
                     LAVDARAPLFGVVGRLVEQKGVDLLTAAAPRLLDGGGQLVVLGSGDPAYEARWKALAE
                     RYRGKLWLRLGFDAALAQRIYAGSDLFLMPSRFEPCGLGQLISFRYGTIPVVHAVGGL
                     AETVRDVDADPRSGNGFSFTRYDVDAFSDAIQRAMNHHLADGAPWRDLVVRVMREDHS
                     WDASARRYADLYARAARLRRTA"
     gene            2325..>3157
                     /locus_tag="VFV20_10150"
     CDS             2325..>3157
                     /locus_tag="VFV20_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288755.1"
                     /translation="MTTIVDLEEEIGRLPAEARAAASRIFTVSTTTGVLDAPPQMHAW
                     IAKLFGSVDAVREQRIVRVTNTVTFEGALFNELRAMRPMEVKGADEVRQTIESARGDP
                     FDHPETGTPADSFGRITGRHGVTASNIAKYDGFHGVLVFNEHDPLAPMDATVLRDHLA
                     TARRWGEAALAADPAAHYLFVMWNCLWRAGGSIVHGHMQMTATRGAHYPKVEALRRQA
                     LAYATSMGRDYFDDLWLVHSALGLGVEIEGARVLASLVPVKEREVLVIGRPGADESSL
                     AP"
BASE COUNT          439 a         1126 c         1077 g          515 t
ORIGIN      
        1 atcttcgcgt tcgcgtcgtt catcagcgtc gacaccagac cgctcttcga tttgccgggc
       61 gtcgcgtccg cgatctcgcc gaggctcttg ccggcgcgaa gctgtgtcat gaggtccttc
      121 tgcgcgatgc cgaggtaggc gcgtgcgtcg ctcatgacgt cgccgatgaa cgcgcgcacg
      181 ctgggcagcc gcggcttcgg cgtcggccag gtgtggtcga cgaacttcgc gacccgctcc
      241 ggcacggacg ccttggcctt gtcggcctgc tcctgcgtga tcttcccgtc cgcgaccgcc
      301 ttcgcgatcg cgtcgttgac cgcgttcacg agcgccgtca cgagaccgtc gcggctcttc
      361 ccggcggtcg cgttcgcgat cgcaccgagg ctcgttccgg gcagcttcgc cttgaggtcg
      421 gccggctgca tgccgaggta ggtcgcgctc tgctggaaga ggccggcgaa gatccgccgg
      481 agcagctctt cccgatcgcg atcgtggctc ttctccttga gcgccgcgag gatcgcgtcc
      541 tcctgctgct gcgtgatgac gcccttgctc acgagcgcat ccaggacgtc cttcagcttg
      601 ttgccgcgtg gcgcctcggt cagcgtgttc gcgcctggct cgggtgccag caccgacacc
      661 gtgtcattcg tcgcggccgg ggcgagcgcg gcgaacgcag cgcttcccag catgctcagc
      721 ccgaagaccg tggtgaccgc gatgaccgcg gtcctgcgtc cgatgagctt catgtgtttg
      781 gtgcctcctt aggcgggatc tcttcctcac ccgtgtatcc acctagctaa cgacatcgtt
      841 tcgcccgggg atacggtggc ggccccccgt catcgactcc ttacatcccg ttaacatcca
      901 catcctcagg tgcggatcgt gtttgcggcg gccgaggccg ccccctacgc gaaggtgggt
      961 ggtcttgcgg atgtggccgg ttcgctcccg caggccctgg ccgcgctcgg ccacgacgtc
     1021 accctctacc ttccgctgca cgcgaccatc gaccgcgcgc gtttcggcat cgaggggtcg
     1081 gggccgacgc ggtccgtggc ctacggcaag agcgccgcgc gcgtggcgta tcccacgatc
     1141 cagcatgacg gcgtccgcgt ggtcttcgtc gacagtgcgc gacgcctcgc gcgcgacaag
     1201 gtgtacgggg cgaaggacga caacaagcgc tacgcgttct tctgccgcgc cgtcttcgag
     1261 gacgtgcgcg aggcgaagcc cgacgtggtg cacgcgcacg actggcacac cgcgctgctc
     1321 gtgccgctcg tgcggcgcgc gcgcagcctc gctcgtaccg cgaccatctt caccatccac
     1381 aacctcgcgt accagggccg cacgtccgcc gagatcctga agctgatcgg cctgccgcgg
     1441 gcccgccttg cgatcgagga tccgggcgag tgcaatccca tggcgcgggg gatcgcgtat
     1501 gccgacatcg tgagcacggt gagccagcag tacgcgaagg agatcctcac gcgcgagttc
     1561 ggcgagcggc tcgatgggct cctccgcgag cggcgacagc atcttgccgg gatcgtgaac
     1621 ggcatcgaca cgaaggcgtt cgatcccgcg accgacgcga acatcgccgc ccgctacgcg
     1681 tccggcgatc cggcggcgaa ggccaaggac aaggaggcgc tgcaggcgga gggcggcctc
     1741 gccgtcgacg cgcgcgctcc gctcttcggc gtcgtcggcc gcctggtcga gcagaagggc
     1801 gtcgacctgc tcaccgccgc cgcgccgcgc ctgctggacg ggggcggcca gctcgtcgtg
     1861 ctcggctccg gcgatccggc gtacgaggcg cgctggaagg cgctcgcgga gcggtaccgc
     1921 ggcaagctct ggctgcggct cggcttcgat gccgcgctcg cgcagcgcat ctacgccggg
     1981 agcgacctgt tcctcatgcc atcgcgcttc gagccgtgcg ggctcggcca gctcatctcg
     2041 ttccggtacg gaacgatccc cgtggtccat gccgtcggcg gcctcgcgga gaccgtgcgc
     2101 gacgtcgacg ccgacccgcg ctcgggcaac ggcttctcgt tcacgcgcta cgacgtcgac
     2161 gccttcagcg acgcgatcca gcgcgccatg aaccaccatc tcgcggacgg cgcgccgtgg
     2221 cgcgacctcg tcgttcgcgt gatgcgcgag gaccattcct gggatgcgtc ggccaggcgc
     2281 tacgccgatc tgtacgcacg agctgcgcga ctgcgacgca cggcatgacg acgatcgtgg
     2341 atctcgagga ggagatcggc cgtctgccgg ccgaggcgcg cgcggcggca tcgcgcatct
     2401 tcaccgtctc gacgacgacc ggcgtgctcg atgctccgcc ccagatgcac gcgtggatcg
     2461 ccaagctgtt cgggtcggtg gacgccgtgc gcgaacagcg catcgtgcgc gtgacgaaca
     2521 ccgtgacctt cgagggcgcg ctcttcaacg agctgcgcgc gatgcggccg atggaagtga
     2581 agggcgccga cgaggtgcgg cagacgatcg agtccgcgcg cggcgacccg ttcgaccatc
     2641 ccgagacggg gacgcccgcc gacagcttcg gccgcatcac gggccggcac ggcgtcacgg
     2701 cgtccaacat cgccaagtac gacggcttcc acggagtgct cgtcttcaac gagcacgatc
     2761 cgctcgcgcc gatggacgcc acggtcctcc gcgaccacct ggcgaccgcg cggcgctggg
     2821 gcgaggccgc gctcgcggcc gatccggcgg cccactacct cttcgtcatg tggaactgcc
     2881 tatggcgcgc gggcgggtcc atcgtgcacg ggcacatgca gatgaccgcc acgcgcggcg
     2941 cccactatcc aaaggtcgag gcgctgcgcc gccaggcgct cgcctacgcg acgtcgatgg
     3001 gccgggacta ctttgacgac ctctggctcg tccacagcgc actgggactc ggcgtcgaga
     3061 tcgagggcgc gcgggtgctc gcctcgctcg tcccggtgaa ggagcgtgag gtactggtca
     3121 tcggacggcc gggcgccgac gagagctcac tcgcgcc
//
LOCUS       DASTZR010000456         4173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1252152, whole genome shotgun sequence.
ACCESSION   DASTZR010000456 DASTZR010000000
VERSION     DASTZR010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252152"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VFV20_10155"
     CDS             <1..337
                     /locus_tag="VFV20_10155"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU5288756.1"
                     /translation="KFAFLFIDDGVDDQANPTPEQQRVYADIFKWFETNGSKIADGGA
                     ELQPTRTATTIRKSGGKSAVVDGPFIEAKESVGGFTIFELPDRNAAVELAKTWPGHGI
                     EVRPIVEER"
     gene            343..684
                     /locus_tag="VFV20_10160"
     CDS             343..684
                     /locus_tag="VFV20_10160"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU5288757.1"
                     /translation="MPQYMLMFVGTDEDWGSRSKEERTQAYAAIGTWWEQLSKQGVIK
                     SGEELQPRRTATTVKRVNGQMKVFDGPFIESKEHVGGYALIQAADLDEALRVAKSWPG
                     GDVEVRPIVER"
     gene            711..1898
                     /locus_tag="VFV20_10165"
     CDS             711..1898
                     /locus_tag="VFV20_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5288758.1"
                     /translation="MAAVFREEAGRLTASLVRSLGDFDLAEECVQDALVSALEHWPRD
                     GVPDNPGAWLMTTARRRGIDRLRRAKRYEQKIAELEDATVATPSTMRDERLELIFTCC
                     HPALSREAQVALTLRSVAGLTTPEIARAFLVSESTVAQRLVRAKRKIVDARIPFRVPE
                     RAELAERLDEVLAVLYLMFNEGYLSTGRRGASDRDLAAEAEWLASLVTELLPEEPEAI
                     GLLALMRLHLARAAARFDRDGRLVLLKDQERWKWDGGRIDDARRLVARFMALGRPGPY
                     QLQAVIALAHADAPAWEETRWPDIVRAYDALLAFGDSPVVRLNRAIAVWHVAGAETAL
                     AELGPLADALDAYHLYHATRAELLRALGRPDEARAADRRALALTENPAERTLLEARIA
                     GDR"
     gene            1922..2197
                     /locus_tag="VFV20_10170"
     CDS             1922..2197
                     /locus_tag="VFV20_10170"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEU5288759.1"
                     /translation="MSVRVLLHGAWRGFAGGAKEVTLQAATVREAMDALAAAHPPLRE
                     RLRDEHGRLRAHLAVFVNDEDARLLGWEDAPLKEGDVIHVIPALSGG"
     gene            2233..3327
                     /locus_tag="VFV20_10175"
     CDS             2233..3327
                     /locus_tag="VFV20_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288760.1"
                     /translation="MAKSVALMVGTKKGLYTLRSTDRKKWTQEGPYGAPAPVHHAAYD
                     PRDGSMYAAINSTWGGARIEYSRDLGKTWTTGKNPKFPEGSDRTFFQTWHIEPGHAST
                     PNVVWAGTEPAGLFKSEDRGETWTLNTALDDVPSRKEWVPGFGGMGLHSIAIDATDPK
                     FMQVGISVGGVYTSKDGGASWERDNQQTGGPGGFSFERKDASDVRPDVAYCIHKLLAH
                     PKVSGVRFMRVHEEVYWREPGDKTWTFRTPGLPSNFGFAAAMHPHDRNTAWCIPLDGQ
                     KRIAEGGIAVYKTSDRGVTWTRQDSGLPKGAPLESLREGMHNDRFDDMGVYFGTANGD
                     VYASLDEGKSWDRIAQYLPYVVSVHAATIE"
     gene            3351..>4173
                     /locus_tag="VFV20_10180"
     CDS             3351..>4173
                     /locus_tag="VFV20_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5288761.1"
                     /translation="MAAAAEVRGLEKRYGDVRAVDGISFSVGEGEVFGLLGHNGAGKT
                     TTIRMLTGRARPTSGTATVAGYDVVRQRDRIKPVINAVFEDPNLYERLGGRDNLRLFA
                     ELYGAPAARADELLDLVGLAGAAKRKVKTYSSGMKQRLLVARALVNRPRVLFMDEPTR
                     GLDPTSARELRQLVAGLAKEGTTIFLTTHYMEEADELCDRVAFLSQGRIVALDTPREL
                     KLKQGERRAVVLLKDRSEHSLRLDDPADAARLDEWMRRDLVLTIHSQEGTLEDVFI"
BASE COUNT          678 a         1407 c         1521 g          567 t
ORIGIN      
        1 caagttcgca ttcctgttca tcgacgacgg ggtggacgac caggcgaacc cgacccccga
       61 gcagcagagg gtctatgccg acatcttcaa gtggttcgag acgaacggct cgaagatcgc
      121 ggacggcggc gccgagctgc agccaacgcg taccgcgacg acgatccgca agtccggcgg
      181 caagtccgcg gtcgtcgacg gcccgttcat cgaggcgaag gagtcggtgg gcggcttcac
      241 gatcttcgag ctccccgacc ggaacgccgc cgtggagctc gcgaagacgt ggccgggcca
      301 cggcatcgaa gtccgcccga tcgtcgaaga gaggtgagcg cgatgccgca gtacatgctg
      361 atgttcgtcg gcaccgacga ggactggggg tcgcgatcga aggaggagcg cacgcaggcc
      421 tacgccgcga tcggcacctg gtgggaacag ctctcgaagc agggcgtgat caagagcggt
      481 gaggagctgc agccgcggcg caccgcgacc acggtgaagc gcgtgaacgg gcagatgaag
      541 gtattcgacg ggccgttcat cgaatcgaag gagcacgtgg gcggctacgc gctcatccag
      601 gcggccgatc tcgacgaggc gctgcgggtg gcgaagtcat ggcccggcgg cgacgtcgag
      661 gtccgcccga tcgtggagcg gtagcgacga tcgccatcac gagcgacgca gtggccgccg
      721 tcttccgcga ggaggcgggc cggctcaccg cgtccctcgt gcgcagcctc ggcgacttcg
      781 acctcgcgga ggagtgcgta caggacgcgc tcgtgtccgc gctcgagcat tggccgcggg
      841 acggcgtgcc cgataaccct ggcgcgtggc tcatgacgac cgcgaggcgc aggggcatcg
      901 accgcctgcg cagagcgaag cgctacgagc agaagatcgc ggagctggag gacgcgaccg
      961 tggcgacgcc gagcacgatg cgtgacgagc ggctggagct gatcttcacc tgctgccacc
     1021 ccgcgctgtc gcgcgaggcc caggtcgcgc tcacgctccg gtcggtcgcg ggtctcacca
     1081 caccggagat cgcccgcgcg ttcctcgtct cggagtcgac cgtcgcgcag cgcctcgttc
     1141 gcgcgaagcg caagatcgtc gacgcgagga tccccttccg cgtgccggag cgcgcggagc
     1201 tcgcggagag gctcgacgag gtcctcgcgg tcctctattt gatgttcaac gagggctatc
     1261 tctccaccgg acggcgtgga gcgagcgatc gcgacctcgc ggccgaggcc gagtggctcg
     1321 cctcgctcgt gaccgagctg ctgccggagg agcccgaggc gatcggactg ctcgcgctga
     1381 tgcgcctcca cctcgcgcgc gcggccgcgc ggttcgaccg cgacggccgg ctcgtgctgc
     1441 tgaaggacca ggaacggtgg aagtgggatg gcggcaggat cgacgacgcg cggcgcctcg
     1501 tggcgcgctt catggcgctc ggacggcccg ggccctacca gcttcaggcg gtcatcgcgc
     1561 tcgcgcacgc ggatgccccc gcctgggagg agacgcgctg gccggacatc gtgcgtgcgt
     1621 acgacgcgct gctcgccttc ggcgactcgc cggtggtgcg cctcaatcgc gccatcgcgg
     1681 tgtggcacgt cgccggcgcc gagaccgcgc tcgccgagct cgggccgctg gcggacgccc
     1741 tcgacgccta ccacctgtat cacgccacgc gcgcggagct gctccgggcg ctcgggcggc
     1801 ccgacgaggc tcgcgccgcc gaccggcgcg cgctcgcgct caccgagaac ccagccgagc
     1861 gcacgctcct cgaggcccgg atcgccggcg accgatgagt tccgaggtgc gcgaccgtcc
     1921 aatgagcgtg agggtgctgc tgcacggcgc ctggcgcggc ttcgccggcg gggccaagga
     1981 ggtcacgctg caggcggcca cggtgcgtga ggccatggac gcgctcgccg cggcccatcc
     2041 tccgctccgc gagcggctgc gcgacgagca tggcaggctg cgcgcacacc tcgcagtttt
     2101 cgtgaacgac gaggacgcgc gactgctcgg ttgggaggac gcaccgctca aggagggcga
     2161 tgtcatacac gtgatcccag cgctctctgg cggctagatc gggacgacgg tcgagtttga
     2221 aggaggacgg aagtggcgaa gtctgtggcg ctcatggtcg ggacgaagaa gggcctctac
     2281 acgctgcgaa gcacggaccg gaagaagtgg acccaggagg ggccctacgg cgcgccggcg
     2341 ccggtccatc acgcggcgta cgacccgcgc gacggctcga tgtacgcggc gatcaactcc
     2401 acctggggcg gagcgcgcat cgagtacagc cgtgacctcg gcaagacgtg gacgacgggc
     2461 aagaacccga agttcccgga gggctcggac cgcacgttct tccagacctg gcacatcgag
     2521 cccggccacg cgagcacgcc gaacgtggtg tgggccggca ccgagccggc gggtctcttc
     2581 aagagcgagg accgcggcga gacctggacg ctcaacaccg cgctcgacga cgtcccgtcg
     2641 cgcaaggagt gggtccccgg cttcggcggc atgggcctgc actcgatcgc gatcgacgcg
     2701 acggatccga agttcatgca ggtcggcatc agcgtgggcg gcgtgtacac gtcgaaggac
     2761 ggcggcgcgt cgtgggagcg cgacaaccag cagaccggcg gaccgggcgg cttttcgttc
     2821 gaacgcaagg acgcttccga cgtgcgcccg gatgtcgcgt actgcatcca caagctgctc
     2881 gcgcatccca aggtgagcgg tgtccgtttc atgcgcgtgc acgaggaggt gtactggcgc
     2941 gagcccggcg acaagacgtg gaccttccgc acgccgggcc tgccctcgaa cttcggattc
     3001 gccgcggcga tgcacccgca cgaccggaac acggcgtggt gcatcccgct ggacggccag
     3061 aagcgcatcg cggagggcgg catcgccgtg tacaagacga gcgaccgcgg cgtgacatgg
     3121 acgcggcagg actccggcct gcccaagggc gcgccgctcg agtcgctgcg cgagggcatg
     3181 cacaacgacc gcttcgacga catgggcgtg tacttcggca cggcgaacgg ggatgtgtac
     3241 gcgagcctcg acgagggcaa gtcctgggac cgcatcgcgc agtaccttcc ctacgtcgtg
     3301 tcggtgcacg cggccacgat cgagtagccg ccgcccgcta cgctccacgc gtggcggcgg
     3361 cggcagaggt ccgcgggctc gagaagcggt acggcgacgt gcgtgccgtc gacgggatct
     3421 cgttctcggt cggcgagggc gaggtcttcg gccttctcgg ccacaacggc gccggtaaga
     3481 ccacgacgat ccggatgctc accggccgcg cgcgtccgac gtccggcacc gcgaccgtcg
     3541 ccggctacga cgtcgtgcgg cagcgcgacc ggatcaagcc ggtcatcaac gcggtcttcg
     3601 aggatcccaa tctgtacgag cggctgggcg gacgcgacaa cctgcgcctg ttcgccgagc
     3661 tctatggcgc gccggccgcg cgcgccgacg agctgctgga cctcgtcggc ctcgccggcg
     3721 cggcgaagcg gaaggtgaag acgtactcca gcggcatgaa gcagcgcctc ctcgtggcgc
     3781 gcgccctggt gaatcgtccg cgcgtgctgt tcatggacga gccgacccgc ggtctggacc
     3841 cgacgtccgc gcgtgagctg cggcagctcg tggccggcct cgcgaaggaa gggaccacga
     3901 tcttcctgac gacgcactac atggaggagg ccgacgagct ctgcgatcgc gtggccttcc
     3961 tgagccaggg ccggatcgtc gcgctcgaca ccccgcgcga gctgaagctg aagcagggag
     4021 agcgccgcgc ggtcgtgctg ctcaaggacc ggagcgagca cagcctgcga ctcgacgatc
     4081 cggcggatgc ggcgcggctc gacgagtgga tgcggcggga cctcgtcctc acgatccact
     4141 cgcaggaagg cacgctcgag gacgtgttca tcg
//
LOCUS       DASTZR010000457         3151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1017267, whole genome shotgun sequence.
ACCESSION   DASTZR010000457 DASTZR010000000
VERSION     DASTZR010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017267"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1569
                     /gene="hutU"
                     /locus_tag="VFV20_10185"
     CDS             <1..1569
                     /gene="hutU"
                     /locus_tag="VFV20_10185"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008358364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEU5288762.1"
                     /translation="NLDPEVAEKPDELIVYGGTGRAARSWEAFDRILATLRRLKGDET
                     LLVQSGKPVGVFRTHEWAPRVLIANALLVPRWATWEKFHELEAAGLTMYGQMTAGSWI
                     YIGTQGILQGTYETFAAVARKHFGGTLAGTLTVTAGLGGMGGAQPMAITMNEGAALVV
                     EVDPARARRRLDQRFLDEIAPSLDDALAVCERHRTAKTPRSVGLVANAADVLPELVRR
                     NVRVDVVTDQTSAHDPLDGYVPAGVADPAALRRADPEAYVRASRASIARHCEAIVALQ
                     QGGAIAFDYGNNLRGEAQTAGFADAFAYPGFVPAYIRPLFAEGRGPFRWVALSGDPKD
                     IARTDRLVTELFPEDETLVRWIRLAGERVPFQGLPARVCWLGYGERALFGERIDELVE
                     RGEISAPIVIGRDHLDSGSVASPFRETEAMRDGSDAIADWPILNALVNTSAGATWVSV
                     HHGGGVGIGNSIHAGMVCVADGTAIGREKLRRVLTTDPGTGVMRHADAGYPEAIRVAR
                     AAGLDLPSIPDTRD"
     gene            1626..2768
                     /locus_tag="VFV20_10190"
     CDS             1626..2768
                     /locus_tag="VFV20_10190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEU5288763.1"
                     /translation="MLMLATFGFVDIWQNGIYGSDFSEIWAGPRVFVSGANPYDPSVW
                     PPAIEALGVQRPGLPVYNYPGWIPILLAPFGAMDLDPAARIWLGLNLFIGAVGFYVLL
                     DQLMPRLPLAFTLFGFALVASEPGIVTFYSGQWDFLIVGLLSLMLVFLRHRRYATAGA
                     FASVMIVKPQLFLVAAPMLLRTTLRRRTARFAVAFLAISGLAALASTIAFPTWWGEYL
                     AVPAAKAGDLRAAALPNAMRDVFGAPGLLAGVALDTLLLAACFRFRAGGAVSTPAWLA
                     ASLVTAPYIFVYDHIVAIVPLALATAIVAERGTAHALLVAALGFAGLVAGATFVHAVP
                     GVAFGSLAVNGFVLYALAALVVLAVWTWRDDDRESPMPARSSTPAK"
     gene            complement(2669..>3151)
                     /locus_tag="VFV20_10195"
     CDS             complement(2669..>3151)
                     /locus_tag="VFV20_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288764.1"
                     /translation="WLGDTTWVRTVVERDEPRILADILWSTAFLRATSLATVVGVAVL
                     GWMSRRSSDETQLHNVALAISPYLAPFAFVWTTFLVMSLPLFASTLARVLASRGPGRT
                     LGVAGIALSLLLMDLVRLHDLVPIAAHFAGVLLLAGIGLSRSSSRQVHTASTTSAASA
                     "
BASE COUNT          416 a         1127 c         1124 g          484 t
ORIGIN      
        1 aacctcgacc cggaggtcgc cgagaagccc gacgagctca tcgtgtacgg gggcacgggg
       61 cgcgccgcgc gatcgtggga ggcgttcgac cgcatcctcg cgacgctgcg gcggctgaag
      121 ggcgacgaga cgctcctcgt ccagtcgggc aagcccgtcg gcgtcttccg cacgcacgag
      181 tgggcgccgc gcgtgctgat cgccaacgcg ctgctcgtgc cgcgctgggc gacgtgggag
      241 aagttccacg agctcgaggc cgcgggcctg acgatgtacg gccagatgac cgcgggctcg
      301 tggatctaca tcggcacgca gggcatcctg caggggacct acgagacgtt cgcggcggtc
      361 gcacgcaagc acttcggcgg cacgctcgcc gggacattga cggtcaccgc gggcctcggc
      421 ggcatgggcg gcgcgcagcc gatggcgatc acgatgaacg agggggctgc gctcgtcgtc
      481 gaggtcgacc cggcacgcgc acgccgccgg ctcgaccagc gcttcctcga cgagatcgcg
      541 ccgtcgctcg acgacgcgct cgctgtgtgc gagcggcatc gcaccgcgaa gacgccgcgc
      601 agcgtcgggc tcgtcgcgaa cgcggccgac gtgctgcccg agctcgtccg gcggaacgtg
      661 cgggtagacg tggtgacgga tcagacgagc gcgcacgatc cgctcgacgg ctacgtgccc
      721 gccggcgtcg ccgatccggc cgcgctgcgg cgcgccgatc ccgaggcgta cgtgcgcgcg
      781 tcacgcgcct cgatcgcccg ccactgcgag gcgatcgtcg cgctccaaca gggcggcgcc
      841 atcgcgttcg actacggcaa caacctccgc ggggaggcgc agacggccgg gttcgccgat
      901 gcgttcgcgt atcccggctt cgtgccggcg tacatccgtc cgctcttcgc cgagggacgc
      961 gggccgttcc gctgggtcgc gctctccggc gacccgaagg acatcgcgcg caccgatcgt
     1021 ctcgtcaccg agctcttccc cgaggacgag acgctcgtcc gttggatccg gctcgcgggc
     1081 gagcgcgtgc cgttccaggg cctgccggcg cgggtgtgct ggctcggcta cggcgagcgc
     1141 gcgctctttg gcgagcggat cgacgagctc gtcgagcgcg gcgagatcag cgcgccgatc
     1201 gtcatcggac gtgaccacct cgactcgggc agcgtcgcat cgccgttccg cgagaccgag
     1261 gcgatgcgcg acggcagcga cgcgatcgcg gactggccga tcctgaacgc gcttgtgaac
     1321 acctcggcgg gtgcgacgtg ggtctcggtg catcacggcg gaggcgtggg gatcgggaat
     1381 tccatccacg ccggcatggt ctgcgtcgcg gacggaacgg cgatcggccg cgagaagctc
     1441 cgccgggtgc tgaccacgga ccctgggacc ggcgtcatgc ggcacgccga cgcgggctat
     1501 ccggaggcga tccgcgtcgc gcgcgcggcc ggcctcgacc ttccgagcat cccggacacc
     1561 cgagactgag cgtccaccct cgggccttca actcgttcgc gtatctgctc ggcctcttcg
     1621 ccgggatgct gatgctcgcg acgttcggct tcgtggacat ctggcagaac gggatctacg
     1681 gctcggactt ctccgagatc tgggcgggtc cacgggtctt cgtctcgggg gcgaatccgt
     1741 acgaccccag cgtgtggcca cccgcgatcg aggcgctcgg cgtccagcga ccggggctgc
     1801 cggtctacaa ctaccccggc tggatcccca tcctcctcgc gccgttcggc gcgatggacc
     1861 tcgatccggc ggcccgcatc tggctggggc tgaacctgtt catcggcgcc gtcggcttct
     1921 acgtcctcct cgaccagctc atgccgcgcc ttccgctcgc gttcacgctg ttcggcttcg
     1981 cgctcgtggc gtccgagccc ggcatcgtca ccttctattc cggacagtgg gacttcctca
     2041 tcgtcgggct cctgtcgctg atgctcgtct tcctgcgtca ccggcggtac gcgacggccg
     2101 gtgccttcgc ctccgtcatg atcgtgaagc cgcagctctt cctcgtcgcg gcgccgatgc
     2161 tgctgcggac cacgctccgc cggcgcaccg cccgcttcgc ggtggcgttc ctcgcgatca
     2221 gcggcctcgc cgccttggcc tcgacgatcg cctttcccac ctggtggggc gaatatctgg
     2281 cggtcccggc ggcgaaggcc ggcgatctcc gcgcggcggc gctgccgaac gcgatgcgcg
     2341 acgtcttcgg cgcgcccgga ctgctcgccg gcgtcgccct cgacacgctg ctcctggccg
     2401 cgtgcttccg gttccgcgcc ggcggcgcgg tgagcacgcc ggcatggctg gcggcgtcgc
     2461 tcgtcaccgc gccctacatc ttcgtgtacg accacatcgt ggcgatcgtg ccgctcgccc
     2521 tcgcgaccgc gatcgtcgcg gaacgcggca ccgcacatgc gctgctggta gcggcgctcg
     2581 gattcgcggg gctcgtcgcg ggcgcgacgt tcgtgcacgc ggtcccgggc gtggcgttcg
     2641 gatcgctcgc cgtgaacggc ttcgtgctct acgcgctggc ggcgctggtc gtgctcgcgg
     2701 tgtggacctg gcgcgatgac gatcgcgaga gcccgatgcc ggcgaggagc agcacgcccg
     2761 cgaagtgagc cgcgatcggc acgaggtcgt gcagccgcac cagatccatc agcagcaagg
     2821 acagcgcgat gcccgcgacg ccgagcgtcc ggccggggcc gcgtgacgcg agcacgcggg
     2881 cgagggtgct cgcgaagagc ggcagcgaca tgacgaggaa cgtcgtccac acgaaggcga
     2941 acggcgcgag atacggcgag atggcgagcg ccacgttgtg gagctgcgtc tcgtcgctcg
     3001 accgacggct catccatccg aggacggcga cgcccacgac cgtcgcgagg cttgtcgcac
     3061 ggaggaaggc ggtcgaccac aggatgtcgg cgaggatcct gggctcgtcg cgctcgacca
     3121 cggtgcgcac ccaggtcgtg tcgccgagcc a
//
LOCUS       DASTZR010000458         6670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_260082, whole genome shotgun sequence.
ACCESSION   DASTZR010000458 DASTZR010000000
VERSION     DASTZR010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6670
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260082"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /gene="rpsB"
                     /locus_tag="VFV20_10200"
     CDS             <1..560
                     /gene="rpsB"
                     /locus_tag="VFV20_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU5288765.1"
                     /translation="TIQRRINRLNELHERKERGDFATMPKKDAQRLQDELEHLDRYFS
                     GVREMKRVPAALFVVDPHREHIAVEEARRLEIPIVAMVDTNCDPDLIDVIIPANDDAI
                     RAVKLICQKMADAVIEGRSMFDASRKEATPEDMGYAPSVTAAEMEGGVGMPRAKRTPR
                     VYEPEEDEEAAIAGAAEADKTPEEI"
     gene            561..1043
                     /gene="tsf"
                     /locus_tag="VFV20_10205"
     CDS             561..1043
                     /gene="tsf"
                     /locus_tag="VFV20_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEU5288766.1"
                     /translation="MSSQVMKKKIDAKEIQRLREETNAGVMDCKRALEDAGGDYEKAK
                     ALLHERGLEKAAKKSEREAKEGVIASYIHANRRVGALVELASETDFVARGDEFQKLAQ
                     EVAMQVAAMAPADMDELMGQAYIRDGSKTVKDLVAAVAMKTGENVSVRRFQRFALGEQ
                     "
     gene            1081..1803
                     /gene="pyrH"
                     /locus_tag="VFV20_10210"
     CDS             1081..1803
                     /gene="pyrH"
                     /locus_tag="VFV20_10210"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU5288767.1"
                     /translation="MTVQPKYKRVLLKLSGEALLGDKKFGISPEYVRYLADEIRKVHE
                     MGVQVAVVIGGGNFMRGAAVAEHGIERATADYMGMLAMVINALALQSVLETLGVSVRV
                     QSAINIAEVAEPYIRRRAIHHLEKGHVVIFAAGTGNPFFTSDTAAALRAAEIGADVIL
                     MGKNAVDGVYDADPNVKKDAKKFEELTHRQVLEKQLGVMDATAAALAADRKTEIIVFD
                     LSQADALVRAVRGEHVGTLVHS"
     gene            1817..2377
                     /gene="frr"
                     /locus_tag="VFV20_10215"
     CDS             1817..2377
                     /gene="frr"
                     /locus_tag="VFV20_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005378949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU5288768.1"
                     /translation="MALEELLAQTEAKFRKAIDALKHEVGGVRTGRAAPSLVESLQVE
                     AYGVDTPLIQLAAISAPEPRMIVIQPWDKSLIKAIEKAITNSDLGLAPNNDGQLIRLN
                     IPALNEERRRDLVKLLHKKVEEGRVEIRTLRRHAHDEMKAKEKSSGVTTDEVKRAEAA
                     LQKLTDRYILIADEIGEAKEKEILEV"
     gene            2406..3071
                     /gene="uppS"
                     /locus_tag="VFV20_10220"
     CDS             2406..3071
                     /gene="uppS"
                     /locus_tag="VFV20_10220"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEU5288769.1"
                     /translation="MAIVMDGNRRWARRRHLPVLAGHRAGVETIRRTLRAARERGVEY
                     LTLYSFSTENWSRDPEEVRGLMGLLEETIRRETATLVKDDVRLEVIGRLHELAPGLQT
                     AIAGAVEATSTCANGVMTLAFNYGGRAEIVDAVRRIVRDGLAPDEIDEAAIASRLYVP
                     GHPDPDLLIRTGGELRVSNFLLWEVAYAEMWATPVLWPDFAVKDLDEALASYAARERR
                     FGR"
     gene            3071..3736
                     /locus_tag="VFV20_10225"
     CDS             3071..3736
                     /locus_tag="VFV20_10225"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU5288770.1"
                     /translation="MGDLGRRTVTAAVYGAVVLVAIVAPPVVFWVALAIVALLGIAEV
                     ARLHAGARGLILGAVFVAGLASLGILRAAGSTGAHHALAGELPVWLLLVLVPTWAADI
                     GAYLVGSTLGRRKLAPRISPGKTWEGTIAGLVLCGLAAFGVGATFGLGRAMVAAVAVL
                     LGIVGLGGDLFESSVKRHAGVKDSGTLLPGHGGVLDRLDSTVTGAIFVAGVFFLGAAS
                     GLG"
     gene            3744..4856
                     /gene="dxr"
                     /locus_tag="VFV20_10230"
     CDS             3744..4856
                     /gene="dxr"
                     /locus_tag="VFV20_10230"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEU5288771.1"
                     /translation="MVTRLAILGATGSIGRQALDVVAANPERLRVSVLAARRSGEDLA
                     VLAQRHGARQVLFERDGGVSALVEAATAKDVDLVLVATSGIDGIEPTLAALEAGKSVA
                     LANKETLVAAGHLVHRLVPGAGDRLRPVDGEHCALWQCLLGVRREELRRVVITASGGP
                     FRTMPLDELARVTPETALRHPTWRMGPKITIDSATLTNKGFEVVEARWLYDLSYDQVD
                     VVVHPESIVHAMIELADGSVKAELAEPDMRLFIQYALLWRHAEAPTRPMDWSAPRTLH
                     FGPVDAERYPCLNTVLEIARSGDLSAMVGLVAADEVAVDRFLRREIGFGEIAGYLHRG
                     AALGRAHGRSAEPSLAEIRHIDAEVRGALRSEPVLS"
     gene            4856..5935
                     /locus_tag="VFV20_10235"
     CDS             4856..5935
                     /locus_tag="VFV20_10235"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEU5288772.1"
                     /translation="MPNIDLRTLLLFIVTFTAIIAVHEFGHYLTARLLGMKVLEFAFG
                     FPPRVAAIEHGGIKYSLNLIPFGGFVRILGQDDFTIEQEGGGDPRSFTAKPWWAQAIV
                     LVAGVTMNIVLALTVLTAAFASGTTAPTGEVKVDQVAEGSPAERAGVLMDDVVVSIDG
                     RTFTRSQDVVTYVRAKARTETEVTLEIRRGGRPIPPIKALPRAEPPEGEGPLGIRLED
                     VQGPVAVALPQAFGEAVSLTGDVVGQIAQLPAQLMASRGGGGGPAVSGPIGIFDVVGQ
                     VSQFGLPTFLKLVGVLSVNLAVLNIIPFPGLDGGRLFFVLVAGIFRKRLSPEVEGVIH
                     AVGFLMLLGLLVVVSFFDIRRIAGG"
     gene            6002..>6670
                     /locus_tag="VFV20_10240"
     CDS             6002..>6670
                     /locus_tag="VFV20_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5288773.1"
                     /translation="MTTSAVSVRRLRKVYRVNERRTGFGATVTSLFRRTYREVPAVGG
                     ISFEIAPGEVVGFLGPNGAGKTTTLKMLSGLLHPTDGEALVNGHVPWRREDDFLRGIT
                     LLMGNRSQLVWDIPAADSFLVLKEIYGIDDATYRGRVDELTELLDLAPLLHKPVRGLS
                     LGERMKVEFAAGLLHGPRIAFLDEPTLGLDVSMQARIRTFVAEYNRRSGATIMLTSHY
                     MDDVV"
BASE COUNT         1025 a         2273 c         2379 g          993 t
ORIGIN      
        1 tgaccatcca gcgccgcatc aaccggctca acgagctcca cgaacgcaag gagcgcggcg
       61 atttcgcgac gatgccgaag aaggacgccc agcgcctgca ggacgagctc gagcatctcg
      121 accggtactt ctccggcgtg cgcgagatga agcgggtgcc ggcggcgctg ttcgtcgtcg
      181 atccgcaccg cgagcacatc gcggtcgaag aggcgcgccg tctggagatc ccgatcgtcg
      241 ccatggtcga cacgaactgc gaccccgacc tcatcgacgt gatcatcccg gcgaacgacg
      301 acgcgatccg cgcggtgaag ctcatctgcc agaagatggc cgacgccgtc atcgaaggcc
      361 gctcgatgtt cgacgcgagc cgcaaggagg cgacgcccga ggacatgggt tacgcgccct
      421 cagtcaccgc cgccgagatg gagggtggcg tcggcatgcc gcgcgcgaaa cgcacgccgc
      481 gcgtgtacga gccggaggag gacgaggagg cagcgatcgc cggcgcagcg gaagccgaca
      541 agaccccgga ggagatctag atgagctcgc aagttatgaa gaagaagatc gacgcgaagg
      601 agatccagcg cctgcgcgag gagacgaacg ccggcgtgat ggactgcaag cgcgccctcg
      661 aggacgccgg cggcgactac gagaaggcga aggcgctcct tcacgagcgc gggctcgaga
      721 aggccgcgaa gaagtccgag cgcgaggcca aggagggcgt gatcgcgtcc tacatccacg
      781 ccaaccggcg cgtgggcgcc ctggtggagc tcgcgagcga gaccgacttc gtcgcgcgtg
      841 gcgacgagtt ccagaagctc gcgcaggagg tcgccatgca ggtcgcggcc atggcgccgg
      901 ccgacatgga cgagctcatg ggccaggcgt acatccgcga cggttccaag acggtgaagg
      961 acctcgtcgc cgcggtggcg atgaagaccg gggagaacgt ctccgtccgc cgcttccaac
     1021 gcttcgccct cggcgagcag tagccccgcg gggaacccga tcgtcgcccg gcctaaactc
     1081 gtgaccgtgc agccgaaata caagcgggta ctgctgaagc tctccgggga ggcgctcctc
     1141 ggggacaaga agttcggcat ctcgcccgag tacgtgcggt acctcgcgga tgagatccgc
     1201 aaggtgcacg agatgggcgt ccaggtggcg gtcgtcatcg gcggcgggaa cttcatgcgc
     1261 ggcgccgcgg tggccgagca cggcatcgag cgcgcgacgg ccgactacat gggcatgctc
     1321 gccatggtca tcaacgcgct cgcgctgcag tcggtgctcg agacgctggg cgtctccgtc
     1381 cgcgtgcagt ccgcgatcaa catcgccgag gtggcggagc cgtacatccg gcgacgcgcg
     1441 atccaccatc tcgaaaaggg acatgtcgtg atcttcgccg cggggaccgg gaacccgttc
     1501 ttcacctccg acacggcggc cgcgctgcgc gccgccgaga tcggcgccga cgtgatcctc
     1561 atggggaaga acgcggtgga cggcgtgtac gacgccgacc cgaacgtgaa gaaggacgcg
     1621 aagaagttcg aggagctcac gcaccgccag gtcctcgaga agcagctcgg cgtgatggac
     1681 gcgaccgccg ccgcgctcgc cgccgaccgg aagaccgaga tcatcgtgtt cgacctctcg
     1741 caggcggacg cgctcgtgcg cgcggtccgc ggcgagcacg tcggcaccct ggtgcactca
     1801 tagtggtggc tcactgatgg ccctcgaaga gctcctcgcc cagaccgagg cgaagttcag
     1861 gaaggcgatc gacgcgctga agcacgaggt cggcggcgtg cgcaccggcc gcgccgcgcc
     1921 gtcgctggtc gagtccctcc aggtcgaggc gtacggcgtg gacaccccgc tcatccagct
     1981 cgccgccatc agcgcgccgg agccgcggat gatcgtgatc cagccgtggg acaagagcct
     2041 catcaaggcg atcgagaagg cgatcacgaa ctccgacctc ggactcgcac ccaacaacga
     2101 cggccagctc atccggctca acatcccggc gctgaacgag gagcgcaggc gcgacctcgt
     2161 gaagctcctc cacaagaagg tggaggaagg gcgcgtggag atccgcacgc tgcgccgcca
     2221 cgcgcacgac gagatgaagg cgaaggagaa gagctcgggc gtgaccaccg acgaggtgaa
     2281 gcgcgccgag gcggcgctcc agaagctcac cgaccgctac atcctcatcg ctgacgagat
     2341 cggcgaggcc aaagaaaaag agatcctcga ggtttagcgt cagcgccgta acggtcccgc
     2401 gccacatcgc gatcgtcatg gacggcaacc ggcgttgggc gcggcggcgg caccttccag
     2461 tcctcgcggg ccaccgcgcg ggggtggaga cgatccgccg cacgctccgc gcggcgcgcg
     2521 agcgcggcgt cgagtacctc acgctgtact cgttctccac ggagaactgg tcgcgcgacc
     2581 ccgaggaggt ccgcgggctc atggggctgc tcgaggagac catccgccgt gagaccgcga
     2641 cgctcgtgaa ggacgatgtg cgtctcgagg tgatcggacg gctgcacgag ctcgcgcccg
     2701 gtctgcagac ggccatcgcc ggtgcggtcg aggccacgtc cacatgcgcg aacggcgtca
     2761 tgacgctcgc cttcaattac ggcgggcgcg cggagatcgt ggacgcggtg cgccgcatcg
     2821 tgcgcgacgg cctcgcgccc gacgagatcg acgaggcggc gatcgcctcg cgcctgtacg
     2881 taccgggtca ccccgatccc gacctcctca tccgcaccgg cggcgagctg cgcgtgtcga
     2941 acttcctcct ctgggaggtc gcgtacgcgg agatgtgggc cacgccggtg ctgtggcccg
     3001 acttcgcggt gaaggacctc gacgaggcgc tcgcctcgta cgccgcgcgc gaacgacgct
     3061 tcggccgctg atgggtgatc tgggccggcg cacggtcacc gcggcggtgt acggcgccgt
     3121 cgtcctcgtc gccatcgtcg cgccgcccgt ggtgttctgg gtcgcgctcg cgatcgtcgc
     3181 gctgctcggc atcgccgagg tcgcccggct gcacgcgggc gcgcgtggcc tgatcctcgg
     3241 tgccgtcttc gtggccggcc tcgcctcgct cggcatcctg cgcgccgccg gatccaccgg
     3301 cgcgcaccat gccctcgcag gcgagctgcc ggtgtggctc ctgctcgtcc tcgtgccgac
     3361 ctgggcggcc gacatcggcg cgtacctcgt cggatccaca ttgggcaggc gaaagctcgc
     3421 gccgcgcatc agccccggga agacctggga gggcacgatc gccgggctcg tcctgtgcgg
     3481 tctcgccgcg ttcggtgtcg gcgccacgtt cgggctcggg cgtgcgatgg tggccgccgt
     3541 cgcggtcctc ctcggcatcg tcggcctggg cggagatctc ttcgagtcct cggtcaagcg
     3601 gcacgcgggc gtgaaggact caggcacgct gctgcccggc cacggtggtg tgctcgaccg
     3661 gctcgacagc accgtaacgg gcgcgatctt cgtcgctggg gtgttcttcc ttggggccgc
     3721 gagcggtctg ggatgatgga cggatggtga cccgactcgc gatcctcggc gccaccggat
     3781 cgatcgggcg gcaggcgctc gacgtcgtcg cggccaatcc cgagcgtctg cgcgtcagcg
     3841 tgctggcggc acgccgcagc ggcgaggatc tcgccgtgct cgcgcagcgt cacggtgccc
     3901 gtcaggtgct cttcgagcgc gatggcggcg tgagcgcgct cgtcgaggcc gcgaccgcga
     3961 aggacgtgga cctggtgctc gtcgcgacga gcggcatcga cgggatcgag ccgacgctcg
     4021 ccgcgctcga ggcggggaag tccgtcgcgc tcgcgaacaa ggagacgctc gtcgccgcgg
     4081 gccacctcgt gcatcgcctg gtgcccgggg cgggcgatcg cctgcggccg gtggacggag
     4141 agcactgcgc cctctggcag tgcctcctcg gcgtgcggcg cgaggagctg cgccgtgtcg
     4201 tcatcaccgc ctcgggcggg ccgttccgca cgatgccgtt ggacgagctc gcgcgcgtca
     4261 caccggagac cgcgctgcgg catcccacgt ggcgcatggg cccgaagatc accatcgatt
     4321 cggccacgct gacgaacaag ggcttcgagg tcgtcgaggc gcgctggctc tacgacctct
     4381 cgtacgacca ggtggacgtc gtcgtccacc ccgagtcgat cgtgcacgcg atgatcgagc
     4441 tcgcggacgg cagcgtgaag gccgagctgg ccgagccgga catgcgcctc ttcatccagt
     4501 acgcgctcct ctggcgccat gccgaggcgc cgacgcgtcc gatggattgg agcgcgccgc
     4561 gcacgctgca ctttggtccg gtggacgccg agcgctatcc gtgcctgaac accgtgctcg
     4621 agatcgcgcg ctcgggcgac ctgtcggcga tggtcggtct ggtcgcggcc gacgaggtcg
     4681 cggtcgaccg gttcctccgg cgcgagatcg gattcggcga gatcgcggga tacctgcacc
     4741 ggggcgcggc gctgggccgc gcgcacggac ggtcggcgga gccgagcctg gcggagatcc
     4801 gccacatcga cgccgaggtg cgcggcgcgc tgcgttcgga gccggtgctc agctgatgcc
     4861 gaacatcgac ctgcgcacgc tcctcctgtt catcgtgacg ttcaccgcga tcatcgcggt
     4921 gcacgagttc ggtcactatc tcaccgcgcg cctcctcggc atgaaggtgc tggagttcgc
     4981 gttcgggttc ccgccgcgcg tcgcggcgat cgagcacggc ggcatcaagt acagcctcaa
     5041 cctcatcccg ttcggcggat tcgtgcggat cctcggacag gacgatttca cgatcgagca
     5101 ggagggcggc ggcgatccgc gatcgttcac cgccaagccg tggtgggcgc aggccatcgt
     5161 gctcgtcgcc ggcgtgacga tgaacatcgt cctcgcgctc acggtgctca ccgccgcctt
     5221 cgcgtccggc acgaccgccc ccaccggcga ggtcaaggtc gaccaggtgg cggaggggtc
     5281 acccgccgaa cgcgccggcg tgctgatgga cgacgtcgtc gtgtcgatcg acggtcgcac
     5341 atttacgcgc tcgcaggacg tcgtgaccta cgtgcgtgcg aaggcccgca ccgagaccga
     5401 ggtgacgctg gagatccggc gcgggggccg tcccatcccg ccgatcaagg cgctgccgcg
     5461 tgccgagccg cccgagggcg agggcccgct cgggatccgc ctcgaggacg tgcagggccc
     5521 ggtcgccgtc gcgctgccgc aggccttcgg cgaggcggtc tcgctcacgg gggacgtggt
     5581 cggtcagatc gcgcagcttc ccgcgcagct catggcgtcg cgaggtggtg gcggcgggcc
     5641 cgcggtgagc ggtccgatcg ggatcttcga cgtcgtggga caggtatcgc agttcgggct
     5701 gccgacgttc ctcaagctcg tcggcgtgct gtccgtgaat ctcgcggtgc tgaacatcat
     5761 cccgttcccc gggctcgacg gcggacgcct cttcttcgtg ctcgtggccg gcatcttccg
     5821 caagcggctt tcgcccgagg tcgagggcgt gatccacgcc gtcggcttcc tcatgctgct
     5881 cggcctgctc gtggtcgtct cgttcttcga tatacggcgc atcgcgggcg gatgagccgc
     5941 ggccggagtg tcgcgcgcgt gatgttcgtg gcgggccgga cgcgctaggc tcggcgtccc
     6001 catgacgacg tcggccgtgt ccgtgcgcag gctgcgcaag gtctaccgcg tgaacgagcg
     6061 gcggaccggc ttcggcgcga cggtcacgtc gctgttccgc cggacgtacc gcgaggtgcc
     6121 cgcggtgggc ggcatctcgt tcgagatcgc gccgggtgag gtcgtcggct tcctcggccc
     6181 gaacggggcg gggaagacca cgacgctgaa gatgctctcg ggactgctcc atccgacgga
     6241 cggcgaggcg ttggtgaacg gtcacgtgcc gtggcgccgc gaggacgact tcctgcgtgg
     6301 catcacgctc ctcatgggga accgctcgca gctggtgtgg gacatccccg cggccgactc
     6361 gttcctcgtc ctcaaggaga tctacggcat cgacgacgcg acgtatcgcg gccgcgtcga
     6421 cgagctcacg gagctcctgg acctcgcgcc gctcctccac aagccggtgc gcgggctgtc
     6481 gctcggcgag cggatgaagg tggaattcgc ggccggactg ctgcacggtc cgcgcatcgc
     6541 gttcctcgac gagcccaccc tcgggctcga cgtctcgatg caggcgcgca tccgcacctt
     6601 cgtcgccgag tacaaccggc ggagcggcgc gacgatcatg ctcaccagcc actacatgga
     6661 cgacgtggtc
//
LOCUS       DASTZR010000459         2430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_495805, whole genome shotgun sequence.
ACCESSION   DASTZR010000459 DASTZR010000000
VERSION     DASTZR010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495805"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="VFV20_10245"
     CDS             complement(<1..862)
                     /locus_tag="VFV20_10245"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5288774.1"
                     /translation="MATTTSTEAAAGFDLTLSPEQEMVQHTAREFAREKVLPLAHEID
                     EHKAVPPEILAEMGRLGFMGIDVPERYGGAGLDALTYVLVVEEINRACASTGVILCSH
                     VSLAIEPILRWGTDAQKERWLPAMARGESLGCFALSEPAAGSDAAAMRTSARQDGDHW
                     VLNGTKNFITNGSKAGVAIVFAQTDPAQRHKGIAAFVVERGTPGFSVGKLESKLGIRG
                     SDTAQLVFEDCRVPAANLLGKVGEGFKIALSTLDGGRISIAAQAVGIARACLEDALAY
                     AKERQAFGKPI"
     gene            complement(840..1211)
                     /locus_tag="VFV20_10250"
     CDS             complement(840..1211)
                     /locus_tag="VFV20_10250"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5288775.1"
                     /translation="MSDLQIGPVMAFSGRRRETATFYRDVVGLAGDDGDDSTWFEALD
                     VKLAVHDQGDRQTPPEIKAAGGFVVWFGVADVRAAFDRARHAGAAASEFHGDYFFARD
                     PDGRYVGIYALEDHHGHDHEH"
     gene            complement(1208..2062)
                     /locus_tag="VFV20_10255"
     CDS             complement(1208..2062)
                     /locus_tag="VFV20_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011173325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEU5288776.1"
                     /translation="MKIGVVGAGTMGSGIAQTFAMHGYDVTMVDVSDAQLARGLTGIR
                     GSLAKLAEKKKLDGAPDEIAGRIRTTTDVGTLADADLVVEAAFEDPAVKRELFATLDR
                     VTKQDVILASNTSSISIGALAGRTSRPARVIGMHFMNPVPLMTLVEVVRGEHTSGDTL
                     DAICDLARTLGKTPVISADRPGFIANRVLMPMINEAFLALDEGVGSAEDIDTVMTLGM
                     NHPIGPLRLADLIGLDVCVAIMEVLERDLASAKYAPAPNLRRYVAEGRLGRKTGRGVY
                     DYRQGATS"
     gene            complement(2063..>2430)
                     /locus_tag="VFV20_10260"
     CDS             complement(2063..>2430)
                     /locus_tag="VFV20_10260"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918779.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEU5288777.1"
                     /translation="ALARVIAQGQYAHEPQLFLSAPRGAIARCLEKAGWTVADVDLFE
                     VNEAFSGVECVRRELDIPDEKFNVNGGAVALGHPIGASGTRLLVTLLYALRARGKRRG
                     IASLCLGGGEAVALAVELV"
BASE COUNT          344 a          878 c          834 g          374 t
ORIGIN      
        1 cgatgggctt cccgaaggcc tgccgctcct tcgcgtaggc gagcgcgtcc tcgaggcacg
       61 cgcgcgcgat ccccacggcc tgcgcggcga tcgagatgcg cccgccatcc agggtcgaaa
      121 gggcgatctt gaagccctcg ccgaccttcc cgagcaggtt cgccgccggc acgcggcagt
      181 cctcgaagac gagctgtgcc gtgtccgatc cgcggatccc gagcttcgac tccagctttc
      241 cgacgctgaa gcccggtgtg ccgcgctcga cgacgaacgc ggcgatgccc ttgtgccgct
      301 gtgccggatc cgtctgcgcg aacacgatcg ccacaccggc cttcgagccg ttggtgatga
      361 agttcttcgt gccgttcagg acccagtgat cgccgtcctg gcgcgcgctg gtccgcatcg
      421 ccgccgcgtc gctccctgcc gccggctcgg agagggcgaa gcagccgagg ctctcgccac
      481 gcgccatcgc cggcagccag cgctccttct gcgcgtcggt gccccaccgc aggatcggct
      541 cgatggcgag gctgacgtgc gagcagagga tgacgccggt gctcgcgcac gcgcggttga
      601 tctcctcgac caccaggacg tacgtcagcg catcgagtcc cgcgccgccg tagcgctcgg
      661 gcacgtcgat ccccatgaat ccgagccggc ccatctccgc gaggatctcg ggcgggacgg
      721 ccttgtgctc gtcgatctcg tgcgcgaggg gcagcacctt ctcgcgcgcg aactcgcgcg
      781 cggtgtgctg caccatctcc tgctcgggcg acagcgtcag gtcgaagccg gccgcggcct
      841 cagtgctcgt ggtcgtggcc atggtggtcc tccaacgcgt agatgccgac gtagcgcccg
      901 tcggggtcgc gcgcgaagaa gtagtcgccg tggaattcgc tcgccgccgc gccggcgtgc
      961 ctggcccgat cgaacgccgc gcgcacgtcg gcgacgccga accatacgac gaaaccgccc
     1021 gccgccttga tctccggcgg cgtctggcgg tcgccctggt catggaccgc gagcttcacg
     1081 tcgagggcct cgaaccaggt ggagtcgtca ccgtcgtcgc cggcaagccc gaccacgtcg
     1141 cgatagaacg tcgcggtctc gcgccgacgg ccgctgaacg ccatcaccgg tccgatctgc
     1201 aggtcgctca cgaggtcgcg ccctgccggt agtcgtacac gccacggccg gtcttgcgtc
     1261 cgagccggcc ctcggcgacg tagcgccgca ggttcggtgc gggcgcgtac ttcgcgctcg
     1321 cgaggtcacg ctccagcacc tccatgatcg cgacacagac gtcgaggccg atgaggtcgg
     1381 cgaggcgcag cggtccgatg ggatggttca tcccgagcgt catcacggtg tcgatgtcct
     1441 ccgcgctgcc gacgccctcg tcgagcgcca ggaaggcctc gttgatcatc ggcatgagca
     1501 cacggttcgc gatgaagccg ggacggtcgg ccgagatgac cggcgtcttg ccgagcgttc
     1561 gcgcgaggtc gcagatggcg tcgagcgtgt cacccgacgt gtgctccccg cgcaccacct
     1621 cgaccagcgt catgagcggg acggggttca tgaagtgcat gccgatcacc cgggcgggac
     1681 gcgacgtccg tcccgcgagc gcgccgatgg agatcgacga ggtgttcgac gccaggatga
     1741 cgtcctgctt cgtcacgcga tcgagcgtgg cgaagagctc gcgcttcacc gccggatcct
     1801 cgaacgcggc ctccacgacg aggtcggcgt cggcgagcgt gccgacgtct gtggtcgtgc
     1861 ggatgcggcc ggcgatctcg tccggcgcgc cgtcgagctt cttcttctcc gcgagcttcg
     1921 cgagggaccc gcggatgccc gtaaggccac gcgccagctg cgcgtcgctg acgtcgacca
     1981 tggtcacgtc gtatccgtgc atcgcgaacg tctgcgcgat gccggacccc atcgtccccg
     2041 cgccgacgac accgatcttc acctagacga gctccacggc gagcgcgacg gcctcgccgc
     2101 cacccaggca gagcgatgcg atgccgcggc gcttgccgcg ggcgcggagc gcgtacagga
     2161 gcgtgaccag caggcgcgtg ccgctcgcgc cgatgggatg gccgagcgcg accgcgccgc
     2221 cgttcacgtt gaacttctcg tcggggatgt cgagctcgcg ccgcacgcac tcgacgccgc
     2281 tgaaggcctc gttcacctcg aagaggtcca cgtcggcgac ggtccagccc gccttctcga
     2341 gacagcgggc gatcgcgccg cgcggcgcgc tgaggaacag ctgcggctcg tgcgcgtact
     2401 ggccctgcgc gatgacgcgc gcgagcgcgg
//
LOCUS       DASTZR010000460         5941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1278596, whole genome shotgun sequence.
ACCESSION   DASTZR010000460 DASTZR010000000
VERSION     DASTZR010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278596"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /gene="ricT"
                     /locus_tag="VFV20_10265"
     CDS             complement(<1..671)
                     /gene="ricT"
                     /locus_tag="VFV20_10265"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HEU5288778.1"
                     /translation="MTTQVIGARFQKAGRMYFFDPAGVTELDLNDWIIVKTDLGLDAA
                     RVQLLSSDSPMLQVDPLLGEVVRKATGADILEINKRKRMEADATRQAADMAVSRRMPL
                     KILGTDWQFDGTSLVVFYSQDTDDRIDLAELIQSYEAHFGTRIELRRLGARDETKVMT
                     GVGTCGRELCCSSWLDKFSNISIRMAKEQDLPLNQAKLTGVCGRLKCCLIYELEVYQE
                     VKGKLP"
     gene            complement(668..1732)
                     /locus_tag="VFV20_10270"
     CDS             complement(668..1732)
                     /locus_tag="VFV20_10270"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HEU5288779.1"
                     /translation="MRDVIGQRRILEKLGEQAIRGDVAHAYELSGPRSIGKHTVALRL
                     AQTLNCGADHPISGGCGTCISCTKIEKGIHPDVLMVTRLVDQVVERGERKGERAADRK
                     NITIEQIRTMQQDLALRPLEGRRRVVIIDDAADLSEHAEVALLKTLEEPPPHAVLLLV
                     TPTPSRLLETIRSRVRPFPFRLVARAEIEAALVARYGREAARFGTVAAGRPGVAFALA
                     SDEASRDARAGLEREFYTLVGSRLTDRFAWAADLADSERDGQKRSEQIESRLLAWGEL
                     VRDAAVVATGATERTLRPERAAQSAKLATAVAARELVDLAQSFERWRRDVVETTVSAR
                     MLLELLALKLPYRADLAAGA"
     gene            complement(1752..2093)
                     /locus_tag="VFV20_10275"
     CDS             complement(1752..2093)
                     /locus_tag="VFV20_10275"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU5288780.1"
                     /translation="MATKTLPERERGVCCTLQLEVPEERAVEIANVLKALADPTRVQI
                     ILALREAKDPSCICDFTATFDLSQPTVSHHMAKLRDAGLVDATRRGIYTYYRLRPHLP
                     AAVARLIEALG"
     gene            2183..2941
                     /gene="arsM"
                     /locus_tag="VFV20_10280"
     CDS             2183..2941
                     /gene="arsM"
                     /locus_tag="VFV20_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HEU5288781.1"
                     /translation="MSELQEAVRARYADRALKMAETPASCCDTATSTCGDGYSAEELA
                     SVGISSTASLGCGNPTLLADLHPGEHVLDLGSGAGLDVLLSAKRVSPGGQAYGVDMTD
                     EMLAVARENQRKAAVANATFLKGTIEQIPLPDAAVDVVISNCVINLAADKGAVLREAF
                     RVLKPNGRLAVADMVAIDELAPEVKKRLDLWAGCIAGTISIDEYTTALANAGFTGIDV
                     EVTRELHLDGVQGRVASAYVRARKPAPVVAESCC"
     gene            complement(2938..4017)
                     /locus_tag="VFV20_10285"
     CDS             complement(2938..4017)
                     /locus_tag="VFV20_10285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5288782.1"
                     /translation="MRIVIAKKLLSTVGGSETFARALDRELPALGHEVSLVGMRPAWE
                     RPGMAEDTRSTLLPVRGGRVGAAIDGLLPTTLVRHEDLRRAVAGADLVHSLAREWAGA
                     LERAARNAGAAFVETPLVHPGQPFSGAGRADVARYRRDDAVVALTEWEASWYRAHGAE
                     RVHVTGVGPNLVSLPEVAREPATILFVGRKERYKGYHALREAAGLVWNERPDARFVAI
                     GQAAWNARFLRPARDERWTELGVVGESEKALAYARATIFAMPSDHETFGHTYLEAWCA
                     GLPVIAGDIPPLREVVREGIDGLHARNEPRAVADAILALLRDPASARRMGENGRLRAM
                     TAYTWPAVARRTSAAYEAALAHARR"
     gene            complement(4022..5164)
                     /locus_tag="VFV20_10290"
     CDS             complement(4022..5164)
                     /locus_tag="VFV20_10290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5288783.1"
                     /translation="MRILFTKRTFAPMGGSESLTYQFATRLAARGHDVRVVCCQAFDV
                     RPRFAQLGVDVVQVKPRGGLLGSLADASTLVDLMPLDELERFAEDRELIHNVGREYLD
                     SSLRPSEELDIPIVLTPLAHPGQFHGGDTPADFERYRRASAITTMTEWERGWYGEHGV
                     DPYRVVTTGMGANATRSSDGAGFRARHRIPADAPIVLYIGRRERYKGFIHLLDAAELV
                     WRHEPETRFVFIGVPGFYGTVVDEFARYTDERIVEIDRSSADEKSAALDACTMYAMPS
                     LHETFGIGYLEAWLHEKPVIGGDIPPLREVIAHGVDGLVVKQKVEDIASAVLTLLADA
                     ELRGRMGAAGHAKLLERWDWERVMDRVEDAYARAVRTHVSADEALA"
     gene            complement(5210..5737)
                     /locus_tag="VFV20_10295"
     CDS             complement(5210..5737)
                     /locus_tag="VFV20_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEU5288784.1"
                     /translation="MAVIDANVLVLNLDFQPLNVCNVRRAVVLLAKEKATVIEQNGHI
                     VTSERLSFPSPSVIRLAYHVKRPRPVVKLTRKEVLQRDDHTCQYCGKRGNDLTLDHVM
                     PRHRGGQHTWENVVAACRSCNHKKGGRTLAESKMQLLRVPRRPPATPTYLYSSYVRGG
                     ERNWAKFIGLDEAAS"
     gene            5777..>5941
                     /locus_tag="VFV20_10300"
     CDS             5777..>5941
                     /locus_tag="VFV20_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005057540.1"
                     /note="with PdxT forms pyridoxal 5'-phosphate from
                     glutamine, either ribose 5-phosphate or ribulose
                     5-phosphate, and either glyceraldehyde 3-phosphate or
                     dihydroxyacetone phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEU5288785.1"
                     /translation="MTSSQNGSNGETRTGTWTLKTGLAQMLKGGVIMDVVTAEHAKIA
                     EEAGAVAVMAL"
BASE COUNT          884 a         2083 c         2075 g          899 t
ORIGIN      
        1 ggcagcttgc ccttcacctc ttgatagacc tcgagctcgt agatgaggca gcacttgaga
       61 cgcccgcaca cgccggtgag ctttgcctgg ttcagcggta ggtcctgctc cttcgccatg
      121 cggatcgaga tattcgagaa cttgtcgagc cacgacgagc aacacagctc acgcccgcag
      181 gtgcccacac cggtcatgac cttcgtctcg tcacgcgcgc cgagccgccg gagctcgatg
      241 cgcgtcccga agtgcgcctc gtagctctgg atgagctccg cgaggtcgat gcggtcgtcg
      301 gtgtcctgcg agtagaagac caccagggac gtgccgtcga actgccagtc ggtcccgagg
      361 atcttcagcg gcatgcgccg cgagaccgcc atgtcggcgg cctgacgcgt ggcgtcggcc
      421 tccatgcgct tgcgcttgtt gatctccagg atgtccgcgc cggtggcctt gcgtacgacc
      481 tcccccagca gcggatccac ctgcagcatc ggcgagtcag acgagagcag ctgcacgcgc
      541 gccgcatcga ggccgagatc ggtcttcacg atgatccagt cgttcaggtc gagctcggtc
      601 acgcccgccg gatcgaagaa gtacatccgg ccggccttct ggaagcgcgc gccgatcacc
      661 tgcgtcgtca tgcccctgcc gccagatccg cgcggtacgg cagcttcagc gcgagcagct
      721 cgagcagcat gcgcgcgctc accgtcgttt cgacgacgtc gcgccgccag cgctcgaagc
      781 tctgggccag gtcgacgagc tcccgcgccg ccaccgccgt cgcgagcttc gcggactgcg
      841 ccgcgcgctc cgggcgcagg gtgcgctcgg tcgcgccggt cgccacgacg gcggcatcgc
      901 ggacgagctc gccccacgcg agaagacgcg actcgatctg ctcgctccgc ttctgaccgt
      961 cgcgctcgga gtcggccagg tcggccgccc aggcgaagcg gtcggtcagg cggctcccga
     1021 cgagcgtgta gaactcccgc tcgagcccgg cgcgcgcgtc gcgcgacgcc tcgtcgctcg
     1081 cgagcgcgaa cgcgacaccg ggccggccgg ctgccaccgt gccgaagcgc gcggcctcgc
     1141 gtccataccg cgccacgagc gcggcctcga tctccgcccg cgcgacgaga cggaacggga
     1201 acggacgcac gcgcgagcgg atcgtctcga ggagacgcga cggggtcggg gtcacgagga
     1261 gcagcaccgc atgcggcggc ggctcctcga gcgtcttgag cagggccacc tcggcgtgct
     1321 cggagaggtc cgccgcgtcg tcgatgatca cgacgcgcct gcgtccctcg agcggccgca
     1381 gggccagatc ctgctgcatc gtgcggatct gctcgatggt gatgttcttc cggtctgccg
     1441 cgcgctcgcc cttgcgctcg ccacgctcga ccacctgatc gacgaggcgc gtgaccatga
     1501 gcacgtcggg gtggatgccc ttctcgatct tcgtgcagga gatgcacgtg ccgcagccgc
     1561 cgctgatcgg gtgatcggcg ccacagttca gcgtctgcgc gaggcgcagc gcaaccgtgt
     1621 gcttgccgat cgatcgcgga ccgctgagct cgtacgcatg ggcgacgtcg ccgcggatgg
     1681 cctgctcccc gagcttctcg aggatgcgac gctgcccaat gacatcacgc atcgggtagc
     1741 gcgattgtag gtcagccgag cgcttcgatg agcctcgcga ccgccgccgg caggtgcggg
     1801 cgcagccggt aatacgtgta gatgccgcgc cgcgtcgcgt ccacgaggcc cgcatcacgg
     1861 agcttcgcca tgtggtgcga gaccgtgggc tgggagaggt cgaacgtggc ggtgaagtcg
     1921 cagatgcagg aggggtcctt cgcctcgcgc agcgcgagga tgatctggac gcgcgtgggg
     1981 tcggcgagcg ccttcaagac gttcgcgatc tcgacggcgc gctcctcggg gacctcgagc
     2041 tggagcgtgc agcacacgcc gcgttcgcgc tcggggaggg tcttggtcgc cacggcatcg
     2101 acgctagcat acccattgac aatcatcaat cggtatgttc gtatagccat cgatatccgt
     2161 ctatgggtat tggaggacag gaatgagcga gcttcaggag gccgtgcgcg cccggtatgc
     2221 ggatcgtgcc ctcaagatgg cggagacgcc ggcgagctgc tgcgacacgg cgacatcgac
     2281 gtgcggggac ggttacagcg ccgaggagct cgcgagcgtc ggcatctctt ccacggcgag
     2341 cctcggctgc gggaacccga cgctcctcgc ggatctgcac cccggtgagc acgtgctcga
     2401 cctcggctcg ggcgccggac tcgacgtcct cctctcggcg aagcgggtct cgcccggcgg
     2461 acaggcctac ggcgtcgaca tgaccgacga gatgctcgcg gtcgcgcgcg agaaccagcg
     2521 caaggctgcc gtcgccaacg cgacgttcct caaggggacg atcgagcaga tcccgctgcc
     2581 cgatgcggcc gtcgacgtgg tgatctcgaa ctgcgtgatc aacctcgccg ccgacaaggg
     2641 cgccgtcctg cgagaggcgt tccgcgtgct gaagcccaac ggccgcctgg cggtcgcgga
     2701 catggtcgcg atcgacgagc tcgcgcccga ggtgaagaag cgtctcgacc tctgggcggg
     2761 ctgcatcgcg ggcacgatct cgatcgacga gtacacgacc gcgctcgcga acgcgggctt
     2821 caccggcatc gatgtcgagg tcacgcgcga gctgcacctc gacggtgtcc agggccgcgt
     2881 cgcgagcgcc tacgtccgcg cgcgcaagcc ggcgcccgtc gtcgcggaga gctgctgcta
     2941 gcggcgagcg tgggcgagcg ccgcctcgta cgcggcgctc gtccgcctcg cgaccgccgg
     3001 ccacgtgtac gcggtcatcg cgcgcaggcg gccgttctcg cccatgcgcc gcgcgctcgc
     3061 gggatcgcgg agcagcgcga ggatcgcgtc ggcgaccgcg cgcggctcgt tccgcgcgtg
     3121 caggccgtcg atcccctcgc gcaccacctc gcgcagcggc gggatgtcgc cggcgatcac
     3181 cggcagaccc gcgcaccacg cctcgaggta ggtatgaccg aaggtctcgt gatcgctcgg
     3241 catcgcgaag atcgtcgcgc gcgcgtaggc gagcgccttc tccgattcac cgaccactcc
     3301 gagctccgtc cagcgctcgt cgcgggccgg ccgcaggaac cgcgcgttcc acgccgcttg
     3361 gccgatcgcg acgaaccgcg cgtccgggcg ctcgttccag acgagccccg ccgcctcgcg
     3421 cagcgcgtgg tagcccttgt accgctcttt gcgccccacg aagaggatcg tcgcgggctc
     3481 gcgcgcgacc tcggggagcg agacgaggtt cgggccgaca ccggtgacgt gcacgcgctc
     3541 cgcgccgtgc gcgcggtacc aggacgcctc ccactccgtc agcgcgacga ccgcgtcgtc
     3601 gcgccggtag cgcgcgacat cggcgcggcc cgcaccggag aagggctggc cgggatgcac
     3661 gaggggagtc tcgacgaagg cggcgcccgc gttccgcgcc gcgcgctcca gcgcgccggc
     3721 ccactcccgc gcgagcgaat gcaccaggtc ggcgccggcg accgcgcgcc gcaggtcctc
     3781 gtggcgcacg agggtcgtcg gcagaagccc gtcgatcgcg gccccgacgc ggccgccgcg
     3841 caccgggagc agcgtcgatc gcgtgtcctc ggccatgccc gggcgttccc acgccggacg
     3901 catcccgacg agggacacct cgtgaccgag tgccggcagc tcgcggtcga gggcgcgcgc
     3961 gaacgtctcc gatccaccga cggtcgagag cagcttcttc gcgatgacga tgcgcaaatg
     4021 cttaggcgag ggcctcgtcg gcgctgacgt gggtgcgcac ggcgcgcgcg tacgcgtcct
     4081 cgacgcgatc catcacgcgc tcccagtccc aacgctcgag cagcttcgcg tgtcccgccg
     4141 ctcccatgcg cccgcgcagc tccgcgtcgg cgagcagcgt gagcacggcg gacgcgatgt
     4201 cctccacctt ctgcttcacc acgaggccgt cgacgccgtg cgcgatcacc tcgcgcagcg
     4261 gcgggatgtc gccgccgatg accggcttct cgtgcagcca ggcctcgagg tagccgatgc
     4321 cgaacgtctc gtgcagcgat ggcatcgcgt acatcgtgca ggcatcgagc gccgcggatt
     4381 tctcatccgc cgacgagcga tcgatctcga cgatgcgctc gtcggtgtag cgcgcgaact
     4441 cgtccacgac ggtgccgtag aagcccggga cgccgatgaa cacgaaccgc gtctcgggct
     4501 cgtggcgcca cacgagctcg gcggcgtcga ggaggtggat gaaacccttg tagcgctcgc
     4561 ggcgaccgat gtagaggacg atcggcgcgt ccgccgggat gcggtgccgc gcccggaatc
     4621 ccgcgccgtc ggacgagcgc gtggcgttcg cgcccatgcc ggtcgtcacg acgcggtagg
     4681 gatcgacgcc gtgctcgccg taccagccgc gctcccactc ggtcatcgtc gtgatcgcgc
     4741 tcgcgcggcg gtagcgctcg aagtccgccg gcgtgtcgcc gccgtggaac tgtccgggat
     4801 gcgccagtgg cgtgagcacg atggggatgt cgagctcctc gctcgggcgc agcgagctgt
     4861 cgagatactc gcgcccgacg ttgtggatga gctcgcgatc ctccgcgaag cgctcgagct
     4921 catcgagcgg catgaggtcg accagcgtcg acgcgtcggc gagggacccg agaagaccgc
     4981 cgcgcggctt gacctgcacg acgtcgacgc ccagctgcgc gaagcgcggc cgcacgtcga
     5041 acgcctggca gcacacgaca cgcacgtcat gaccgcgggc ggccagtcgc gtcgcgaact
     5101 ggtacgtcag gctctcggag ccgcccatcg gagcgaacgt ccgcttggtg aacagaatgc
     5161 gcatcctgat cgcctctctc ttgcatggga cctgccgggg cgcgccggat tacgaggcgg
     5221 cttcgtccag gccgatgaac ttcgcccagt tgcgctcgcc gccgcgaacg tatgacgagt
     5281 acagataggt cggcgtggcg ggcgggcgcc tgggcacgcg gagtagctgc atcttcgact
     5341 cggcgagcgt gcggccgccc ttcttgtggt tgcacgaccg gcaggccgcg accacgttct
     5401 cccaggtgtg ctgaccgccg cgatgtcggg gcatgacgtg gtcgagcgtc aggtcgttgc
     5461 cccgcttgcc gcagtactgg caagtgtggt cgtcccgctg gaggacctcc ttgcgcgtca
     5521 gcttcaccac cggccgcggc cgcttcacgt ggtaggcgag gcggatgacg gagggggagg
     5581 ggaagctcag ccgctcgctc gtcacgatgt ggccgttctg ctcgatgacc gtggccttct
     5641 ctttggcgag cagcacgaca gcgcgccgga cgttgcacac gttcagcggc tggaagtcca
     5701 ggttgagcac gaggacgttc gcgtcgatga ccgccatgtt cagattctag ggtccgcgcg
     5761 gtacgattgc gtacgtatga cctcttctca gaacggttcc aacggcgaga cgcgcacggg
     5821 cacctggacc ctgaagaccg gcctcgccca gatgctcaag ggcggcgtga tcatggacgt
     5881 cgtcaccgcc gagcacgcga agatcgcgga ggaggcgggc gccgtcgcgg tgatggcgct
     5941 c
//
LOCUS       DASTZR010000461         6963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_286680, whole genome shotgun sequence.
ACCESSION   DASTZR010000461 DASTZR010000000
VERSION     DASTZR010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6963
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286680"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="VFV20_10305"
     CDS             <1..1021
                     /locus_tag="VFV20_10305"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEU5288786.1"
                     /translation="ASLTLLAFLTYAAIGLAYIAGTPYHADAVVAPHRAAELLLQGRE
                     PYAAFDMPEALARFGLDPELNTHLVDGSVLRTYSYPALSFLVVTPFVALGLGDIRWIY
                     LTLVLILAALVSARLRPAWRPFAVATVFGSEVIARQNVLAGIDPTWALLLLGAWIARR
                     RPWLSGALLGLAIADRQTAWFAAPFVLVGVANERGAREAIRRGVVALGVFLLVHAPFV
                     IASPARTVDSVLGPLVQPLVSDGVGLMRFGVRDLYSPPFAREVYTALALVALAALLLL
                     LWRRPRAVSSAPLVWPFLTLYFAWRSLQNYFAFAGPFALAADDELMDGPAVTPAVRGD
                     VAPRA"
     gene            complement(981..2081)
                     /locus_tag="VFV20_10310"
     CDS             complement(981..2081)
                     /locus_tag="VFV20_10310"
                     /inference="COORDINATES: protein motif:HMM:NF019522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEU5288787.1"
                     /translation="MRRLAFVLLAALTLACGPLVEGGRADVVAGHVVEVLEIGDRGQL
                     GSGMPQPFQRLRVQLDESLYRGEVVELQWGGRRALDPNGFLRAGDRVLLSVTREGNDR
                     TYAILEVVRLPALAPFAVLLLLALLAVARIKGLAALAGLAASVSVFLLAIVPALQRGD
                     DPLVATLVGSLGVIAVSVFVVHGFNRKSLAALCGTMAGLGVVTAIGALALATARMTGM
                     GAEDQIFLAVGTDGRIDMPRLALAAVMVGSLGAIVDMSVGQSSAVSELAAVDETLRGR
                     RLYASALNVGRDHIGSLVNTLALAYFGGALPLVLLISLGYQPLSIAINSEEILASVIA
                     IIAASLGLVLCVPITTAIAVMLAGPRPPEPPE"
     gene            complement(2101..2517)
                     /locus_tag="VFV20_10315"
     CDS             complement(2101..2517)
                     /locus_tag="VFV20_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288788.1"
                     /translation="MPPPPAARSLDPNADVVVTYQVAGGGTIRFTVRPRYVAGEPVTL
                     TVDVGAGSVPIRGPLSGRILGSGLEGEKVIRTFGPGTLAPVEVAADRAARTTVLWDGR
                     DDDGAPVRADTYSAAFDFVVGGESVRFGSVIQLVRP"
     gene            complement(2561..3286)
                     /locus_tag="VFV20_10320"
     CDS             complement(2561..3286)
                     /locus_tag="VFV20_10320"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEU5288789.1"
                     /translation="MNLLVVITAVVTFLSTLAGGIVALRATTHVGLVIALGAGVRIGA
                     AFFDLAPESARELGSLDSAMLWAGIGFLAFYMLERLTLLHVGHEHGQVLDRHEHVGIL
                     GAGGMSVHSFLDGVAIGAAFHAGAEIGLLVALVVVLHDFSDGIGTVSVLVANSASRQT
                     AIRWLFVDAAAPMLGALATFALTLDSSVLGALLGIFVGFFLYVGGAELLPEAHRKERS
                     DWVMIATVAGAVFIYAATRLVQI"
     gene            3325..3738
                     /locus_tag="VFV20_10325"
     CDS             3325..3738
                     /locus_tag="VFV20_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288790.1"
                     /translation="MSERLEAALAGELVDAMLEPRSADLLDAPAPLADEMVMMRRGAR
                     RVSNAPRTVLHPVDAREDAERHEQVQRPEHGCSADAAPLELRDDVLSGERLRAAQRGA
                     DHEGARRRAAAAIRAQTVEDGGRSCGGHFVETRYH"
     gene            complement(3780..4691)
                     /locus_tag="VFV20_10330"
     CDS             complement(3780..4691)
                     /locus_tag="VFV20_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288791.1"
                     /translation="MDLSTLALLLGMVALGFRHGFDWDHIAAITDITSTTTAGHSEMD
                     VPASAPVTPHGHDARELRPHVAHAGPPGRVHIFGESRFAHEQRHAIGLASLYALGHAS
                     VVFVLGVSALLVGAVLPAWVDPILEKVVGVTLVLLGAWVLYSVVQYLRGKGEFRMRSR
                     WMLVFDLVRHGWGALQARIHGHEHHPSLHATQYGPKTAFGVGMIHGIGAETGSQALLI
                     AGIAGVSGATGIVILAAFIVGLIASNTLVAVLSASGFIGAQRMRVVYVIVGALAGSAS
                     LLIGLLFIGGLGTVLPDLQELLFGGSR"
     gene            4783..5235
                     /locus_tag="VFV20_10335"
     CDS             4783..5235
                     /locus_tag="VFV20_10335"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEU5288792.1"
                     /translation="MATAGERRGDTADVAERVRASGRRMTVQRRLVLQALQRARHHTT
                     AEDVAARIRRQHPQIDPSTVYRNLEALEALGYVTHTHFEDRVTRWHLADERHGHLVCR
                     SCGEEIEIAMGVLEPLARRLRLEHGFEADLAHSAVVGVCRACASVSSP"
     gene            5360..5944
                     /locus_tag="VFV20_10340"
     CDS             5360..5944
                     /locus_tag="VFV20_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288793.1"
                     /translation="MAAATLVVVASLWWSAGTAYAHERRNVGPYLLVVGWLNEPAFAG
                     QLNAATVRVSDPRENPAKAVEGLEKTLKIDVASGGLAPFTGAVRAVFGQPGLYALDMY
                     PTVAGKYRYVVTGTIGTTNVNETFESSPTTFNDVQETSPLQYPSKVPTGDDLNTRLDA
                     IRSTVDQTRLIAIAALVLGVVALGGAVLAGRRRT"
     gene            6004..>6963
                     /locus_tag="VFV20_10345"
     CDS             6004..>6963
                     /locus_tag="VFV20_10345"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEU5288794.1"
                     /translation="MAMAHANYVKSSPASDARLVRSPSEVRVTFSETPDPKGSDIAVL
                     DTSGARRDGDDVATATDEQNTLRVSVPVLPEGGYLVAWTARSSVDGHETKGAFAFAIG
                     NAPLPSIPDVGPATAPPAPLELAGRALSFAGIAIVLGMGFFALFIRAPDEPRARRRER
                     ALVLGGGALLIAGSAVLLVAYGADIPARLLLFVALRALAGAVAIAAVLAPSRLLPAGT
                     RREVIAFAGLAAALWATLVSHAAANGDPRYIALDLIHVVAISVWSGGVVALLLVAIPA
                     VTDVRALGATVWRFSLTALVAVAVIVATGVVQSLDRLVLMEDLV"
BASE COUNT         1034 a         2516 c         2430 g          983 t
ORIGIN      
        1 cgcgtcgctg acgctcctcg cgttcctcac gtacgccgcg atcggcctcg cgtacatcgc
       61 ggggacgccg taccacgccg acgctgtggt cgcgccccat cgcgccgcgg agctgctcct
      121 gcagggccgc gagccgtacg ccgcattcga catgcccgag gcgctggctc gtttcgggct
      181 cgacccggag ctcaacacgc acctcgtcga tgggagcgtg ctgcgcacct attcgtaccc
      241 cgcgctgtca ttcctcgtcg tcacgccgtt cgtcgcgctc ggcctcggcg acatccgctg
      301 gatctacctc accctggtgc tgatcctcgc cgcgctcgtg tccgcgcggc tgcggccggc
      361 gtggcggccg ttcgcggtcg cgacggtctt cggcagcgag gtcatcgcgc gccagaacgt
      421 cctggccggc atcgatccca cgtgggcgct gctgctcctc ggtgcgtgga tcgcgagacg
      481 ccgcccgtgg ctctccggag cgctgctcgg cctcgcgatc gcggatcgtc agacggcctg
      541 gttcgccgct ccgttcgtcc tcgtcggcgt ggcgaacgaa cgcggcgctc gcgaggccat
      601 ccgccgcggc gtcgtggcgc tcggtgtgtt cctcctggtg cacgccccgt tcgtgatcgc
      661 gtcgccggca cggaccgtcg acagcgtcct cggcccgctc gttcagccgc tcgtctccga
      721 cggcgtcggc ctcatgcgct tcggcgtgcg tgacctgtac agcccgccct tcgcgcgcga
      781 ggtgtacacc gcgctcgcgc tcgtggcgct cgccgcgctg ctgctcctgc tctggcgtcg
      841 gccgcgtgcg gtgtcgagcg cgccgctggt ctggccgttc ctcacgctgt acttcgcgtg
      901 gcgctcgctg cagaactact tcgcgttcgc cggacccttc gcgctggccg cggacgacga
      961 gctgatggac ggtcccgcgg tcactccggc ggttcggggg gacgtggccc cgcgagcatg
     1021 accgcgatcg ccgtcgtgat gggcacgcaa agcaccaggc cgaggctcgc cgcgatgatc
     1081 gcgatgacgg acgcgaggat ctcctcgctg ttgatcgcga tcgagagcgg ctggtagccg
     1141 agcgagatca ggagcaccag cggcagcgcg ccgccgaaat acgcgagcgc gagcgtgttc
     1201 acgagagagc cgatgtggtc gcgacccaca ttgagcgccg acgcatagag gcgacggccg
     1261 cggagtgtct cgtcgacggc cgcgagctcc gataccgcgg acgactggcc gacgctcatg
     1321 tccacgatcg cgccgagcga tccgaccatg accgccgcca gcgcgaggcg cggcatgtcg
     1381 atccgtccgt cggtgccgac tgcgaggaag atctgatctt cggcgcccat ccccgtcatc
     1441 cgtgccgtgg cgagcgcgag cgcgccgatc gccgtcacca cgcccagccc cgccatcgtg
     1501 ccgcacagtg ccgcgaggct cttccggttg aacccgtgca ccacgaacac ggagacggcg
     1561 atgacgccga gcgagccgac gagcgtggcc accagcggat catcgccgcg ctgcagcgcc
     1621 gggacgatcg cgagcaggaa gaccgacacg ctcgccgcga gcccggcgag cgcggcgagc
     1681 ccctttatgc gcgcgaccgc gagcagcgcg agcaggagga gcaccgcgaa cggcgccagc
     1741 gcggggagcc ggacgacctc gaggatcgcg tacgtacggt cgttcccctc gcgcgtgacg
     1801 ctgagcaaca cgcggtcgcc ggcgcgcagg aaaccgttgg ggtcgagggc gcggcgcccg
     1861 ccccactgga gctcgacgac ctcgccgcga tacaggctct cgtcgagctg gacgcgcagg
     1921 cgctggaacg gctgcggcat gccgctgccg agctggccac ggtcgccgat ctcgagcacc
     1981 tcgacgacgt gaccggcgac cacgtccgcg cggccaccct cgaccagagg tccgcacgcc
     2041 agcgtgagcg cggcgaggag cacgaacgca agacgcctca cgacgagcac accgtatcag
     2101 tcagggtcgg acgagctgga tcacgctgcc gaaccgcacc gactcgccgc ccaccacgaa
     2161 gtcgaacgcg gcgctgtacg tatcggcgcg gaccggcgcg ccgtcgtcat cgcgcccgtc
     2221 ccagaggacc gtcgttcgcg ccgcgcggtc ggccgcgacc tcgacgggtg cgagcgtccc
     2281 gggtccgaac gtgcgtatga ccttctcgcc ttcgagcccg ctcccgagga tccggccgga
     2341 cagcgggccg cggatcggca ccgatcccgc gcccacgtcc acggtgagcg tgaccggctc
     2401 tcccgcgacg tagcgcgggc ggaccgtgaa gcggatcgtc cctccgcccg cgacctggta
     2461 cgtgacgacg acgtcggcgt tcgggtcgag cgaacgcgcg gccggcggtg gcggcatgca
     2521 tgtgatggcg gccacaagga ccgccgccac gatgaaggcg ttagatctgg acgagccgcg
     2581 tcgcggcata gatgaatacg gccccagcga cggtcgcgat catgacccaa tccgatcgct
     2641 ccttccggtg cgcctcgggg aggagctccg cgccgccgac gtacaggaag aatccgacga
     2701 agatgccgag gagcgctccg agcacggagc tgtcgagcgt cagcgcgaag gtcgcgagcg
     2761 cacccagcat gggcgcggcc gcgtcgacga acagccaccg gatggcggtc tggcggctcg
     2821 ccgaattcgc gaccagcacg ctgacggtgc cgatcccatc ggagaagtcg tgcagcacga
     2881 cgacgagtgc gacgagcaga ccgatctcgg cgcccgcgtg aaaggccgcg ccgatcgcga
     2941 cgccgtcgag gaagctgtgc accgacatgc cgccggcacc gaggatgccg acgtgctcat
     3001 gccggtccag cacctggccg tgttcgtggc ccacgtgcag caacgtgagg cgctcgagca
     3061 tgtagaacgc gaggaatccg atgccggccc agagcatcgc gctgtcgagc gagcccagct
     3121 cgcgcgccga ttcgggcgcg aggtcgaaga acgcggcgcc gatgcggaca ccggcgccga
     3181 gcgcgatgac cagccccaca tgcgtggtgg cacggagtgc gacgatcccg cccgccagcg
     3241 tggagaggaa cgtgaccacc gcggtgatga cgaccagaag gttcatcgac ggcaccgcgc
     3301 gcacaacccg ttcacctcga gaccgtgtcc gagcggctcg aagccgcgct cgcgggcgag
     3361 ctcgtcgatg cgatgctcga gccccgaagc gccgatctcc tcgacgcgcc cgcacccttg
     3421 gcagatgaga tggtgatgat gcgtcggggc gcacgccgtg tatcgaacgc accgcgtacc
     3481 gtgctccatc ccgtggatgc gagagaggac gccgagcgac acgagcaggt ccagcgtccg
     3541 gaacacggtt gctctgccga cgccgcgccg cttgagctcc gcgacgatgt cctgagcggt
     3601 gaacggctgc gcgcggcgca gcgcggcgcc gatcacgaag gcgcgcgacg tcgtgcggcg
     3661 gcggccatcc gcgcccagac ggtggaggac ggcggtcgtt cctgcggcgg tcactttgtt
     3721 gagacacggt atcactaggc gcggcaacgc gcgcggccgg accggcgcgt tcgatggggc
     3781 tagcggctgc ctccgaacag cagctcctgc aggtccggca gcaccgtgcc gagaccgccg
     3841 atgaacagca gcccgatgag gaggcttgca gatccggcga gcgcgccgac gatcacgtag
     3901 accacgcgca tgcgctgcgc gccgatgaat ccgctcgcgc tcagcaccgc gacgagcgtg
     3961 ttcgaggcga tcagccccac gatgaaggcg gcgaggatca cgatgcccgt cgcgccgctc
     4021 acacccgcga tccccgcgat gagcagcgcc tgcgagccgg tctcggcacc gatgccgtgg
     4081 atcatcccca cgccgaacgc cgtcttcggt ccgtactgcg tggcgtggag cgaagggtgg
     4141 tgctcgtgtc catggatgcg ggcctgcagc gctccccacc cgtggcggac gagatcgaag
     4201 acgagcatcc agcggctgcg catccggaac tcgcccttcc cgcgcaggta ctgcaccacg
     4261 gagtagagca cccacgcgcc gaggagcacc agggtcaccc cgacgacctt ctcgaggatc
     4321 gggtcgaccc aggctggcag caccgctccc acgagcagcg cggacacgcc gagcacgaac
     4381 accaccgacg cgtgcccgag ggcgtacaag ctcgcgagtc cgatcgcatg gcgctgctcg
     4441 tgcgcgaacc gtgactcgcc gaagatgtgc acgcgcccgg gcggtcccgc gtgcgccacg
     4501 tgcgggcgaa gctcgcgggc atcgtgcccg tgcggtgtca cgggcgcgct ggcgggcacg
     4561 tccatctcac tgtgaccggc cgtcgtcgtg ctcgtgatgt cggtgatcgc ggcgatgtgg
     4621 tcccagtcga agccgtgccg gaagccgagc gcgaccatgc cgagaagcag cgcgagcgtc
     4681 gaaagatcca tcggtcggag cgtaccgagc gagcaggctt atcgcaacga gccgcaatag
     4741 cgtcctcttg cacatcgttc gcagtcgcga gagagttgcg gtatggcgac ggcgggcgag
     4801 cggcgcggag acacggccga cgtggccgag cgggtgcgcg cgagcgggcg gcggatgacg
     4861 gtgcagcgcc gcctcgtgct ccaggcgttg cagcgcgcac ggcatcacac cacagcggag
     4921 gacgtcgcgg cgcgcatccg gaggcagcac ccgcagatcg acccatcgac ggtgtaccgg
     4981 aacctcgaag cgctcgaagc actcggctac gtcacgcaca cgcacttcga ggaccgtgtg
     5041 acgcgatggc atctcgccga cgagcggcac ggtcatctcg tgtgccggtc ctgcggcgag
     5101 gagatcgaga tcgccatggg cgtgctcgag ccgctcgcgc gacggctgcg cctcgagcac
     5161 ggcttcgagg ccgacctcgc tcactccgcg gtggtcggcg tctgccgcgc gtgcgcgtcc
     5221 gtatcctctc cgtaggtgcc tggggcttcc gcgcgaccga gcccgtcgga gccgctccac
     5281 gcggcctgac acaggtccgg cgcagtcgcg ccggacactc gtcatcgtgg agggaagcaa
     5341 gatgtatcga gcgttgtccg tggcggcggc caccctggtc gtcgtcgcga gcctctggtg
     5401 gagcgccggc accgcgtacg cgcacgagcg ccgcaacgtc ggcccctatc tcctcgtcgt
     5461 cggctggctg aacgagccgg cattcgcggg ccagctgaac gccgcgacgg tccgcgtctc
     5521 cgacccgcgc gagaacccgg ccaaggcggt cgagggcctc gagaagacgc tgaagatcga
     5581 cgtcgcgagc ggcggcctcg cgcccttcac cggcgcggtg cgcgcggtct tcggacaacc
     5641 gggcctgtac gcgctcgaca tgtacccgac cgtcgcgggc aagtatcgct acgtcgtcac
     5701 cggcaccatc ggcacgacga atgtgaacga gacgttcgaa tcgagtccca cgacgttcaa
     5761 cgacgtgcag gagacctcgc cgctgcagta cccatccaag gtgccgaccg gtgacgacct
     5821 caacacgcgg ctcgacgcga tccgctccac cgtcgatcag acccggctca tcgcgatcgc
     5881 ggcgctcgtg ctcggcgtgg tcgccctcgg cggtgccgtt ctggcgggac ggcggcgcac
     5941 ctgagctccc gcgcagccgc gctcgcgctc ggcgtactcg gctccctgct cttctcgggc
     6001 gggatggcga tggcgcacgc gaactacgtg aagtcgagcc cggcctcgga cgcgcgactg
     6061 gtacggtcgc cgtcggaggt gcgcgtcacc ttcagcgaga cgccggaccc gaagggatcc
     6121 gacatcgccg tcctcgacac atcgggtgcg cgccgcgacg gtgacgacgt agcgacggcc
     6181 accgacgagc agaacacgct gcgcgtgtcg gtcccggtgt tgccggaggg tggctatctc
     6241 gtggcgtgga ccgcgcgatc gtcggtcgac ggccacgaga cgaaaggcgc cttcgccttc
     6301 gccatcggaa acgcgccgct tcccagcatc ccggacgtcg gtccagccac cgcgccgccg
     6361 gcaccgctcg agctcgccgg gcgcgcgctg tcgttcgccg gcatcgcgat cgtcctgggc
     6421 atggggttct tcgcactgtt catccgcgcg ccggacgagc ctcgcgcgcg ccgtcgggag
     6481 cgtgcgctcg tgctcggcgg cggcgcgctg ctcatcgccg gcagcgccgt gctcctcgtc
     6541 gcgtacgggg ccgacatccc ggcccgcctg ctgctgttcg tcgcgctccg cgcgctcgcc
     6601 ggcgccgtcg cgatcgctgc cgtcctcgcg ccgtcgcggc tcctgccggc cgggacccga
     6661 cgcgaggtga tcgcgttcgc cgggctcgcc gcggcgctct gggcgacact cgtctcgcac
     6721 gcggccgcga acggcgaccc gcgatacatc gcgctcgacc tcatccacgt cgtcgcgatc
     6781 tccgtctgga gcggcggggt cgtcgcgctg ctcctcgtgg cgatcccggc cgtcaccgac
     6841 gtacgcgcgc tcggcgcgac ggtgtggcgg ttctcgctga ccgcgctcgt cgcggtggcc
     6901 gtgatcgtcg ccaccggcgt ggtgcagtcg ctcgaccgac tcgtgctcat ggaggacctc
     6961 gtc
//
LOCUS       DASTZR010000462         2090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_260167, whole genome shotgun sequence.
ACCESSION   DASTZR010000462 DASTZR010000000
VERSION     DASTZR010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260167"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VFV20_10350"
     CDS             <1..685
                     /locus_tag="VFV20_10350"
                     /inference="COORDINATES: protein motif:HMM:NF037084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase type III"
                     /protein_id="HEU5288795.1"
                     /translation="ALDYVATELERGIKEGKKLQAAVKSLLTKMIKENKRIIFNGNGY
                     SDEWQRDASKRGLLNLRTSVDAFPELMKPDVVQAFEKYGVLNERELRARYEVAHEQYN
                     KTINIEANLMVLMGNRYILPAAYRYQSEVAQSVTAVKAAGGSAKEARRTLDTVCRLTD
                     ELKVRIDHLQELLDHEANGDAEKHAKYFRDKVVPAMNALRETGDSIECVMPHDLWPLP
                     TYREMLFVK"
     gene            689..1438
                     /locus_tag="VFV20_10355"
     CDS             689..1438
                     /locus_tag="VFV20_10355"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEU5288796.1"
                     /translation="MADGVVWQVFWVAVLTDLATGLGALPFAFVRHFSEAWKGMMTAA
                     AAGMMLSASVFTLTGEALQAGAVWEVLVGLLAGAAFFAFTARLVHGSDWTIGSMSVAD
                     SRQSLLVIVAMFVHSIPEGIAIGVGYATGELRFGLLLATAIAVHNIPEGVAVSMPLRA
                     KGFSVASCTGYAILTSLPQPVAAVPAYLLVSVFKPLLPFGLGFAGGAMIFLVISELLP
                     DSLECCTREQSAWAMMIGLTAMLWITGTLIQ"
     gene            complement(1523..2090)
                     /locus_tag="VFV20_10360"
                     /pseudo
     CDS             complement(1523..2090)
                     /locus_tag="VFV20_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010967605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
BASE COUNT          319 a          728 c          700 g          343 t
ORIGIN      
        1 ggcgctcgac tacgtcgcga cggagctcga acgcgggatc aaggaaggca agaagctcca
       61 ggcggcggtg aagtcgctgc tcaccaagat gatcaaggaa aacaagcgga tcatcttcaa
      121 cggcaacggc tactcggacg agtggcagcg cgacgcctcc aagcgcggcc tcctcaacct
      181 ccgcacctcg gtggacgcgt tccccgagct gatgaagccg gacgtcgtgc aggcgttcga
      241 gaagtacggc gtgctcaacg agcgcgagct gcgcgcgcgc tacgaggtcg cgcacgagca
      301 gtacaacaag accatcaaca tcgaagccaa cctcatggtg ctcatgggga accgctacat
      361 cctccccgcg gcgtaccggt accagtcgga ggtcgcccaa tcggtgacgg ccgtgaaggc
      421 ggccgggggc agcgcgaagg aagcgcggcg gacgctcgac acggtctgcc ggctcaccga
      481 cgagctcaag gtgcgcatcg atcacctgca ggagctcctc gaccacgagg cgaacggcga
      541 cgcggagaag cacgcgaagt acttccgcga caaggtcgtg cccgcgatga acgcgctgcg
      601 cgaaacgggc gacagcatcg agtgcgtgat gccgcacgac ctctggccgc tcccgacgta
      661 tcgtgagatg ctattcgtga agtagttgat ggcggatggc gtcgtttggc aggtcttctg
      721 ggttgcggtc ctgacggacc tggcgacggg cctcggcgca cttccgttcg cgttcgttcg
      781 gcacttctcc gaggcgtgga agggcatgat gacggcggcg gccgccggca tgatgctctc
      841 cgcgtccgtc ttcaccctca cgggtgaggc gcttcaggcg ggcgccgtct gggaggtcct
      901 cgtcgggctg ctcgccggcg ccgcgttctt cgccttcacg gcgcggctgg tccacggcag
      961 cgactggacg atcgggtcca tgtccgtcgc ggactcgcga cagagcctcc tcgtcatcgt
     1021 cgcgatgttc gtccacagca tccccgaggg catcgccatc ggcgtcggct atgccaccgg
     1081 ggagctgcgg ttcggcctgc tgctcgccac ggccatcgcg gtccacaaca ttcccgaggg
     1141 cgtcgcggtg tcgatgccgc tgcgcgcgaa gggattctcg gtggcgagct gcaccggtta
     1201 cgcgatcctc acgagcctgc cgcagccggt cgccgcggtg cccgcgtacc tcctggtctc
     1261 cgtgttcaag ccgctgctgc cgttcggcct cggctttgcc ggcggcgcga tgatcttcct
     1321 cgtcatctcc gagctcctgc ccgacagcct cgagtgctgc acgcgcgagc agtcggcgtg
     1381 ggccatgatg atcggactga cggcgatgct ctggatcacc gggacgctca tccagtagcg
     1441 cccggcgccg gcatcagcac tcgtcgtcgt acgcgatgcc cgagggctcc ggttccggct
     1501 ccgggtgcgc gaccgagacc cgcagcgcga tcttcatcac gccttcgcgc tgcgcggcga
     1561 acaggtcgta cgcgtcctgg atccgatcga acgggaggtg gtgcgtcacg atgggcgaga
     1621 ggtccacgcg gtgcgcgacg atcagctgca tgagccgccg catccgctcc ttgccgcccg
     1681 ggcagagtgt cgtgacgatc tgtttgtcgc cgaggcccgc gagcagcgcc tcgtacggcg
     1741 ccgcgatctt gcccgagtag acgccgaggc tcgagagcgt gccgccgggt cggatcgcct
     1801 tcagcgcgtt ctcgaacgtc tcctgccggc cgagcgcctc gatcgccacg tccacgccgc
     1861 ggccgtgcgt cagcgccctg atgatgggca gcggatcctc ctccgccgcg ttgacggtga
     1921 tgttcgcccc gaaccgccgc gccatctcga gccgctcgtc gatcatgtcg atcgcgatga
     1981 tgaggccggc cccgcgcagc ttcgcccccg ccgtcgcgca caggccgatg ggcccctccg
     2041 cgaacaccgc gacgctgtcg ccggcccgga ttcccgcccg ttccgccccc
//
LOCUS       DASTZR010000463         2488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1148155, whole genome shotgun sequence.
ACCESSION   DASTZR010000463 DASTZR010000000
VERSION     DASTZR010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148155"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..358
                     /locus_tag="VFV20_10365"
     CDS             170..358
                     /locus_tag="VFV20_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288797.1"
                     /translation="MGCCLAGCLRLIFFQIWRLAAAAILAMLLARVDDYVERRYGDRT
                     VGRAYRAYRKRGKGDTSV"
     gene            366..1502
                     /locus_tag="VFV20_10370"
     CDS             366..1502
                     /locus_tag="VFV20_10370"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEU5288798.1"
                     /translation="MASRVDVFRIPTRILFGRGVALTVAEPLRQVGAKRVLLVTDKGV
                     LGAGLVAPIEERLRDAKIPYEIYDEVVPDPGVGEVQRCYERARAAGADAFVAVGGGSS
                     IDTAKMAATLLVNGGTVLDYIGIDKVPKPAAPVVAIPTTAGTGAEITINSVIADPAQH
                     KKLVIISPNATALFALEDPDMTRTLPPFGTAITGMDTLVHAIESFVNKQTYRTTELLA
                     LEAIRDAGRALPRAVKDGGDIEAREAMLRAVVNASLAFSNTRLGNVHAMALPLGGWCH
                     VAHGTAVAILLPHVMDFNRVAAEERYAQVGEALGVAKDAKAAVRRVFDLNAEIGIPAG
                     LRTVGVTDDAIPNMTKDAMLSGNIAVNPRTTTAADVEALYRAAL"
     gene            1586..2203
                     /locus_tag="VFV20_10375"
     CDS             1586..2203
                     /locus_tag="VFV20_10375"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU5288799.1"
                     /translation="MLLLHAWWGLNDDVRAYAKRLREEGFAVEMPDLYGDGRTADTIA
                     GAEKLRDELESSWPDFSPGTPLGRARGAIDAAAARLAESGPYGIVAWSLGAFHGWELV
                     KRGVGRPAAFVVHYGIYDVGDVSGADAPEVLGHVAEQDQFDPPEAFAALEAALRQEGV
                     TVTHHTYPNTRHWFAEPSRPEYDRAAAELAWTRTLDLLRRRLGQR"
     gene            complement(2213..>2488)
                     /locus_tag="VFV20_10380"
     CDS             complement(2213..>2488)
                     /locus_tag="VFV20_10380"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEU5288800.1"
                     /translation="PVATMDGGPVTISLAEHYFDPSLIAVTVGTTVIWRNVGQQSHDV
                     NARDDSFRSGTLGPGMTFAYTFTKPGRYAYYCIPHEGDGMFGEVDVR"
BASE COUNT          376 a          873 c          871 g          368 t
ORIGIN      
        1 gtccagcgtg cgacctcgag gacgtcggcg agcacgtcat cgtcaagcgg acgctcggcg
       61 aagcgccgga cgacacgcat cgagcgcagg agtgccgtat ccgagcggcg cacgccgcca
      121 ttgtcgtccg cgggcaccgg cgtggcacac gaatcgctag agtcgggatg tgggttgttg
      181 tctggccgga tgcctccggc tgatcttctt ccagatctgg cgtctcgccg cggcggcgat
      241 cctcgcgatg ctgctcgcgc gcgtcgatga ctacgtcgaa cgacgctacg gcgaccgcac
      301 cgtcggccgg gcctaccgcg cgtaccgcaa acgcggcaag ggcgacacct ccgtatagtc
      361 cccgcgtggc ctctcgcgtc gacgtcttcc gcatcccgac gcgcatcctg ttcgggcgcg
      421 gcgtggcgct cacggtcgcg gagccgctcc gccaggtcgg cgcgaagcgc gtgctcctcg
      481 tcaccgacaa gggcgtcctc ggtgccggtc tcgtcgcgcc catcgaggag cgtctgcgcg
      541 acgcgaagat cccgtacgag atctatgacg aggtcgtccc ggatcccggc gtcggcgagg
      601 tgcagcgctg ctacgagcgc gccagggccg cgggggcgga cgcgttcgtc gccgtcggcg
      661 gcggctcgtc gatcgacacg gcgaagatgg cggcgacgct cctggtgaac ggcggcacgg
      721 tgctcgacta catcggcatc gacaaggtgc cgaagccggc agcaccggtg gtcgcgatac
      781 cgaccaccgc gggcaccggc gcggagatca ccatcaactc ggtcatcgcc gaccccgcgc
      841 agcacaagaa gctcgtgatc atcagcccga acgcgacggc cctgttcgcg ctcgaggatc
      901 ccgacatgac gcgcacgctg ccgccgttcg gcaccgcgat caccggcatg gacacgctgg
      961 tgcacgcgat cgagtcgttc gtgaacaagc agacctaccg cacgaccgag ctgctcgcgc
     1021 tcgaggcgat acgcgacgcc gggcgcgcgc tcccgcgcgc ggtgaaggac ggcggcgaca
     1081 tcgaggcgcg cgaggccatg ctgcgcgcgg tcgtgaacgc gagcctcgcg ttctcgaaca
     1141 cgcggctcgg caacgtccac gcgatggcgc tcccgctcgg cggctggtgc cacgtcgcgc
     1201 acggtaccgc ggtcgcgatc cttcttccgc acgtgatgga cttcaaccgg gtcgcggccg
     1261 aggagcgcta cgcacaggtc ggcgaggcgc tcggggttgc gaaggacgcg aaggccgcgg
     1321 tgaggcgcgt cttcgacttg aacgccgaga tcggcatccc ggccgggctg cgcacggtcg
     1381 gcgtcaccga cgacgcgatc ccgaacatga cgaaggacgc gatgctctcg gggaacatcg
     1441 cggtgaatcc gcgcacgacg accgccgcgg atgtcgaggc gctctaccgg gccgcgctct
     1501 aactcgatgc ctccttgtca ctcggctgtc ggtctcgctc ctcgcagatg aactgctcgt
     1561 cgctcggctt gctctgatgc ccggcgtgct gctgctccac gcctggtggg gcctcaacga
     1621 cgacgtccgc gcctacgcga agcggctgcg cgaggagggt ttcgcggtcg agatgcccga
     1681 tctgtacggc gacgggcgca cggcggacac catcgccgga gcggagaagc tccgcgacga
     1741 gctggagagc tcctggcccg acttctcgcc gggcacgccg ctcggtcgcg cccgcggcgc
     1801 catcgatgcg gccgcggcgc gcctggccga aagcggaccg tatgggatcg tcgcgtggag
     1861 cctcggggca ttccacgggt gggagctggt caagcgtggc gtggggcggc ccgccgcatt
     1921 cgtcgtccat tacggcatct acgacgtcgg tgacgtgtcg ggagccgatg cgccggaggt
     1981 cctcggccac gtcgcggagc aggaccagtt cgacccgccg gaggcgttcg ccgcgctcga
     2041 ggcggcgctt cggcaagagg gcgtgaccgt cacccaccac acctatccga acacgcgcca
     2101 ctggttcgcg gagccgagcc ggcccgagta cgaccgcgcc gccgccgagc tcgcgtggac
     2161 gcggacgctg gatctccttc gtcgccgtct cggccagcgc tagcgctacg cgttaccgga
     2221 cgtcgacctc gccgaacatg ccgtcgccct catgggggat gcagtaatac gcataccggc
     2281 ccggcttcgt gaacgtgtac gcgaacgtca tgccgggtcc gagcgtgccg gagcggaacg
     2341 agtcatccct cgcgttcacg tcgtgcgact gctggccgac gttgcgccag atcaccgtcg
     2401 tgccgaccgt cacggcgatc agcgacggat cgaagtagtg ctcggccagc gagatcgtca
     2461 ccgggccgcc gtccatcgtg gcgaccgg
//
LOCUS       DASTZR010000464         5952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_25526, whole genome shotgun sequence.
ACCESSION   DASTZR010000464 DASTZR010000000
VERSION     DASTZR010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5952
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25526"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VFV20_10385"
     CDS             <1..270
                     /locus_tag="VFV20_10385"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU5288801.1"
                     /translation="ERPTPHETGMVPARPLGTVEKCTFCVHRLAEDQVPSCVWSCPAQ
                     ARIFGDLNDPNGRVARLARDGRVLLEDAGTRPSVVYLAPRRKRDL"
     gene            267..1556
                     /gene="nrfD"
                     /locus_tag="VFV20_10390"
     CDS             267..1556
                     /gene="nrfD"
                     /locus_tag="VFV20_10390"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEU5288802.1"
                     /translation="MTAGALPVAVTRARTGAPPALAAWIALLIVIVAAGLAAFAYQFA
                     NGLVVTGLRNTMMWGNYILFFMFFVGLSAGGLIVASAGRLFGISAFKPITRLAVVEAT
                     IAVMLAGIFIIPDLGRPERVLNILLYANLTSPMVWDITIVVAYTVLSMSYVWLYTRED
                     LARRGSWLAFGTGVSEREHVRDERLKSALAWVALPAAILLHSITAWIFGLQISRTFWY
                     SAIMAPLFIASALVSGLALVILLALALRRLRRIEFDDRLVSYLGALLAVFVVVEAFFV
                     GSEFLTAAYPGVPTDADPIRRLLFGPYAPLFWSEVVVGLAVPFAILAARAGRRRPALV
                     GVAAVLAVVGIFIHRLNLLLNGLSYVPIPLPPGVAIGAPQAGTPQSFAAYHWYVPTAV
                     EWLIVGGVLAFGALLFTLAGMYMPLRERPAHAASEMR"
     gene            1641..2540
                     /locus_tag="VFV20_10395"
     CDS             1641..2540
                     /locus_tag="VFV20_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013556212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5288803.1"
                     /translation="MGNGATSAVRLDHVTKRYGAVLALDAIQLDIHAGEFVALLGPNG
                     AGKTTAISLMLGLRAPTDGSVQLLGGRPTDRAIRRRCGVMLQDSGLPPMLRVGEVVRL
                     FRAHYPHPRTVEAVLRLADLWTKRDRRVDALSGGERQRLNYAIAIAGDPEVLFLDEPT
                     VGMDVAARHAFYAQLRELSGSGRTIVLTTHYLEEADRLANRVVVIDHGRVIVDGTPGE
                     IKARVPAKKVALAAGRALTVVDFAGLPVEGLALHDHSASFLSNDAVAVVSALIRHDVP
                     LKDLEVTGAALEDAFLALTERKA"
     gene            2537..3325
                     /locus_tag="VFV20_10400"
     CDS             2537..3325
                     /locus_tag="VFV20_10400"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5288804.1"
                     /translation="MSVAVRLPAGSFPRMLAEHVRAQLLSFTRNTALSAVSVVMPVLL
                     YALVGMSNAAREQAPGVTFGAYFLASMAAYAVSSIMVFNFGVTVAVERGQKVDLLIRA
                     APLPPILYMLARVVTALAFALVALIVLFAFAYVAGGVRLDPAVWLDLGVVLLAGSIPF
                     VAFGFAIAYLSGPNAAVAIANITYMMLAFASGIFIPFEQLPAFVQSVAPYLPTYHFAQ
                     LAWSAVGIAGESRLVSAAWLVAFTAAFFAVAVYAYRRDTGRRFS"
     gene            complement(3318..3989)
                     /locus_tag="VFV20_10405"
     CDS             complement(3318..3989)
                     /locus_tag="VFV20_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288805.1"
                     /translation="MPKANGIDVIVETGQKRCFASALEWPGFARGGRNEDEALAALVA
                     YAPRYARVARKFGFTAPVDTSSLRVVERLAGDASTDFGTPGQKPKADAGHVDDAELER
                     LFAWLEAGWAAFDAGARKAKGKTLRLGPRGGGRSAAKIVEHVREAELAYLAALGVKVD
                     EDVRRDAKRQRAAIREGLRASARGEIPAKGPRGGIRWKPRYFVRRDMWHLLDHLWEIE
                     DRAVS"
     gene            4145..5848
                     /locus_tag="VFV20_10410"
     CDS             4145..5848
                     /locus_tag="VFV20_10410"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU5288806.1"
                     /translation="MRQSNLFGRTLREAPADAQTPGTQLMLRAAIARPLAAGLYTWLP
                     LGFRIARKVEQIIREEQDRIGAQEIRIPVITPAEYWKETGRWDALEPVTFRTKDHGGH
                     EYMIGYTHEELDMHHARSEILSYRQLPAMVYHFQEKGRDEQRPRAGLLRTREFVMKDA
                     YSIDKDWEGLDKQYWAQHGAYARTFERLGLDAISVESDTGNMGGDVAHEFQVLTDVGE
                     DRIAICPKCDYKANMEKATRAVPKAAAAANVAKPTTVATPGVTSIEALTQSLHVGERD
                     LLKTLLMRDAAGNVVAVVLPGDREVNEPKLRKLLQTSDVKFANEADFAAAKAVAGFIG
                     PVGLNARVIVDVSVEPRSYVAGANVKDAHLRDVVPGRDFDGERADLHDVREGDACPKC
                     GGTLTIRRGVEVGNIFKFGPYYADKMNATYLAEDGTKKPLMFGSYGIGVGRSVQTIME
                     THHDDKGLIWPLAVAPFHAHVITLPANDDAVRMAAEALVDELEDAGVEVLYDDRDETA
                     GVKFADADLIGIPFRLTVSKRTIKENAVEVKRRTEEKGALVPSDQVLARMRELLAPPT
                     A"
BASE COUNT          838 a         2145 c         2025 g          944 t
ORIGIN      
        1 gagcgcccga cgccgcacga gaccggcatg gtccccgctc gtccgctggg tacggtcgag
       61 aagtgcacct tctgcgtgca tcgtctcgcg gaggaccagg tcccatcgtg cgtgtggtcg
      121 tgcccggcgc aggcccgcat cttcggcgac ctgaacgatc cgaacggccg cgtcgcccgg
      181 ctcgcgcgcg acgggcgcgt gctgctagag gacgccggca cgaggccctc ggtcgtgtat
      241 ctcgcgccgc gccggaagcg cgacctatga ccgccggagc gctgcccgtc gcggtcaccc
      301 gcgcccgtac cggcgcgccg ccggcgctcg cggcgtggat cgcactcctc atcgtcatcg
      361 tcgcggcggg cctcgccgcg ttcgcgtacc agttcgcgaa cgggctcgtc gtgaccggac
      421 tgcggaacac gatgatgtgg gggaactaca tcctcttctt catgttcttc gtgggcctgt
      481 cggcgggcgg tctcatcgtg gcgtccgcgg gacggctctt cggcatttcc gcgttcaagc
      541 cgatcacgcg gctcgccgtc gtcgaggcga cgatcgcggt catgctggcc ggcatcttca
      601 tcatccccga cctcggccgg ccggagcgcg tcctcaacat cctgctctac gcgaacctca
      661 cgtcgccgat ggtgtgggac atcaccatcg tggtcgcgta cacggtgctg tccatgtcat
      721 acgtctggct ctacacgcgc gaggacctcg cgcggcgggg gagctggctc gccttcggga
      781 ccggcgtgtc cgagcgcgag cacgtgcgcg acgagcgcct gaagtccgcg ctggcgtggg
      841 tcgcgctccc cgcggcgatc ctcctccact ccatcaccgc gtggatcttc ggcctgcaga
      901 tcagcaggac cttctggtac tccgcgatca tggctccact gttcatcgcg tccgcgctcg
      961 tctccggtct cgcgctggtg atcctgctgg cgctggcgct gcgccgcctg cgacgcatcg
     1021 agttcgacga ccggctcgtc tcgtatctcg gcgcgctcct cgcggtcttc gtcgtcgtcg
     1081 aggcgttctt cgtcggctcg gagttcctca cggccgcgta tcccggcgtg ccgaccgatg
     1141 ccgatccgat ccggcgcctc ctcttcggac cgtacgcgcc gctcttctgg tccgaggtcg
     1201 tggtcggcct cgccgtgccc ttcgcgatcc tcgccgcgcg tgcgggccgg cggcgccccg
     1261 cgctcgtggg cgtcgcggca gtcctggcgg tcgtgggcat cttcatccac cgcctgaacc
     1321 tgctgctcaa cggcctctcg tacgtcccga tcccgctgcc gcccggcgtc gcgatcggcg
     1381 cgccgcaggc cggcacgccg cagagcttcg cggcgtacca ctggtacgtc ccgaccgcgg
     1441 tggagtggct catcgtcggc ggcgtcctcg ccttcggcgc gctgctcttc acgctggccg
     1501 gcatgtacat gccgctgcgc gagcgccccg cccacgctgc gtccgagatg cgttagccga
     1561 ggactgggac aaaccctacg ttcctgtcgg acccagcctg acaggcgtga aagcctgggc
     1621 accgcatcgt gcgagccgca gtggggaacg gagcgaccag tgcggtccgg ctcgaccacg
     1681 tcacgaagcg ctacggggcg gtcctcgcgc tcgatgccat ccagctcgac atccacgccg
     1741 gtgaattcgt cgcgctgctc gggccgaacg gcgccggcaa gacgacggcg atcagcctga
     1801 tgctgggact ccgcgcgccg acggacgggt ccgtgcagct cctcggcgga cggcccaccg
     1861 atcgcgccat ccggcggcgg tgcggcgtga tgctccagga ctccggcctg ccgccgatgc
     1921 tgcgtgtcgg cgaggtcgtc cggctcttcc gtgcgcacta cccgcacccg aggaccgtgg
     1981 aggcggtcct gcgcctggcg gacctctgga ccaagcgcga ccggcgcgtc gatgcgctct
     2041 ccggcggcga gcggcaacgg ctcaactacg cgatcgcgat cgcgggcgat cccgaggtgc
     2101 tcttcctcga cgagccgacg gtcggcatgg acgtggccgc gcggcatgcc ttctacgcgc
     2161 agctgcggga gctgtcgggg tcgggacgga cgatcgtcct cacgacgcac tacctcgagg
     2221 aagcggatcg cctcgcgaac cgtgtcgtcg tcatcgacca cggtcgcgtg atcgtcgacg
     2281 ggacaccggg cgagatcaag gcgcgcgtcc cggcaaagaa ggtcgctctc gccgccggtc
     2341 gtgccctcac cgtcgtcgac ttcgcgggcc ttcccgtgga gggattggcg ctgcacgacc
     2401 acagcgcgtc gttcctcagc aacgacgcgg tcgcggtggt ctcggcgctc atccggcacg
     2461 acgtgccgct gaaggatctc gaggtcaccg gcgccgcgct cgaggacgcg ttcctcgcgc
     2521 tcacggagcg gaaggcatga gcgtcgccgt ccggctgccg gcggggtcct ttccgcgcat
     2581 gctcgcggaa cacgttcgag cgcagctcct gtcgttcacg cggaataccg cgctcagcgc
     2641 cgtctcggtc gtcatgcccg tcctgctcta cgcgctcgtc ggcatgtcga acgccgcgcg
     2701 ggagcaggct ccgggcgtca ccttcggcgc gtacttcctc gcgtcgatgg cggcgtacgc
     2761 ggtgagcagc atcatggtgt tcaacttcgg cgtgacggtc gcggtcgagc gcgggcagaa
     2821 ggtggacctg ctcatccgtg ctgcgccact cccgccgatc ctgtacatgc tcgctcgcgt
     2881 ggtcacggcg ctggccttcg cgctcgtcgc cctcatcgtg ctcttcgcgt tcgcgtacgt
     2941 ggccggcggc gtgcggctcg atcccgcggt gtggctcgac ctcggcgtcg tgctcctcgc
     3001 gggctcgatc cccttcgtcg cgttcggatt cgcgatcgcc tacctgtccg gcccgaacgc
     3061 cgccgtggct atcgcgaata tcacctacat gatgctggcg ttcgcgtcgg gcatcttcat
     3121 cccgttcgag cagctgcccg cgttcgtgca gagcgtcgcg ccgtacctgc cgacgtacca
     3181 cttcgcgcag ctcgcgtgga gcgccgtcgg catcgccggc gagtcacggc tggtgagcgc
     3241 cgcctggctc gtcgcgttca cggccgcgtt cttcgccgtc gcggtgtacg cgtatcgacg
     3301 cgacacgggc cgacgcttca gctgacggcg cggtcctcga tctcccagag gtgatcgagg
     3361 agatgccaca tgtcccgacg tacgaagtag cggggcttcc aacggatgcc gccacgcggg
     3421 cccttcgcgg ggatctcgcc gcgcgcggac gcgcgcagac cctcgcggat cgcggcgcgc
     3481 tggcgcttcg cgtcccggcg gacgtcctcg tcaaccttca cgccaagcgc cgcgaggtag
     3541 gcgagctcgg cctcacgcac gtgctcgacg atcttcgcgg cgcttcgacc gccgccgcgc
     3601 ggaccgagcc gcagcgtctt gcccttcgcc ttgcgtgcgc ccgcgtcgaa cgccgcccag
     3661 ccggcctcga gccacgcgaa gagacgctcg agctcggcgt cgtcgacgtg gcccgcatcg
     3721 gccttcggct tctggccggg cgtgccgaaa tcggtggacg cgtcgcccgc gaggcgctcg
     3781 acgacgcgca gcgaggacgt gtcgaccggc gcggtgaatc cgaacttccg cgcgacgcgc
     3841 gcgtaccgcg gcgcgtaggc gacgagcgcc gcgagggcct catcctcgtt cctgccgccc
     3901 cgtgcgaagc ccggccactc gagcgctgac gcgaagcagc gcttctggcc cgtctcgacg
     3961 atcacgtcaa tcccgttcgc cttcggcacg gcgcgcacgc tatcgcatca cgccgcggag
     4021 cgccgtacac tcgccgcata tgcacgtgtc ccgagggtcc acgttccgaa tcccgcgcta
     4081 gccggcgtcc tcgtggcgcc ggcgtcccgc agcgcgtgag aatacgaccc gcggagttgt
     4141 tcacatgaga cagtcgaacc tctttggccg cacccttcgc gaagcgccag ctgacgctca
     4201 gacgcctggc acgcagctca tgctgcgggc cgcgatcgcg cggccgctcg ccgcgggcct
     4261 ctacacctgg cttccgctcg gcttccgcat cgcgcgcaag gtcgagcaga tcatccgcga
     4321 ggagcaggac cgcatcggcg cgcaggagat ccgcatcccg gtcatcacgc cggccgagta
     4381 ctggaaggag accggacgct gggacgcgct cgagccggtg accttccgca cgaaagacca
     4441 cggcgggcac gagtacatga tcggctacac ccacgaggag ctggacatgc accacgcgcg
     4501 gagcgagatc ctctcgtacc ggcagctccc ggcgatggtc taccacttcc aggagaaggg
     4561 ccgggacgag cagcgtccgc gcgcgggcct gctccgtacc cgagagttcg tcatgaagga
     4621 cgcgtactcg atcgacaagg actgggaggg cctcgacaag cagtactggg cgcagcacgg
     4681 cgcgtatgcg cgcacgttcg agcgtctggg cctcgacgcg atcagcgtcg agagcgacac
     4741 cgggaacatg ggcggcgacg tcgcgcacga gttccaggtg ctcaccgacg tcggtgagga
     4801 ccgcatcgcc atctgtccga agtgcgacta caaggcgaac atggagaagg cgacgcgagc
     4861 ggtgcccaag gcggcggcgg ccgcgaacgt cgcgaagccc acgaccgtcg ccacgcccgg
     4921 cgtcaccagc atcgaggcgc tcacgcagag cctgcacgtc ggcgagcgcg acctgctgaa
     4981 gacgctcctc atgcgggacg cggccggcaa cgtcgtcgcc gtggtcctgc cgggcgaccg
     5041 tgaggtgaac gagccgaagc tgcggaagct cctccagacg tcggacgtga agttcgcgaa
     5101 cgaagcggac ttcgcggcgg cgaaggccgt cgccgggttc atcggcccgg tcggcctcaa
     5161 cgcgcgggtc atcgtcgacg tctcggtcga gccgcgttcc tacgtggccg gcgcgaacgt
     5221 gaaggacgcg cacctccgcg acgtcgttcc cggtcgcgac ttcgacggcg agcgcgcgga
     5281 ccttcatgac gtgcgcgagg gcgacgcgtg tccgaagtgc ggtggcaccc tcaccatccg
     5341 ccgtggcgtc gaggtgggga acatcttcaa gttcggcccg tactacgccg acaagatgaa
     5401 cgcgacgtac ctggccgagg acggcacgaa gaagccgctc atgttcggta gctacgggat
     5461 cggggtcggc cgctccgtgc agacgatcat ggagacgcac cacgacgaca agggcctgat
     5521 ctggccgctc gcggtagcgc cgttccacgc gcacgtgatc accctgcccg cgaacgacga
     5581 cgccgtgcgc atggccgcgg aggccctcgt cgacgagctc gaggatgcgg gcgtggaggt
     5641 cctgtacgac gaccgcgacg agaccgcggg cgtgaagttc gccgacgccg acctcatcgg
     5701 catcccgttc cgcctgacgg tgtcgaagcg cacgatcaag gagaacgcgg tcgaggtgaa
     5761 gcgccgcacc gaggagaagg gcgcgctcgt tccgagcgac caggtcctcg cgaggatgcg
     5821 cgagctgctc gcgccaccga cggcgtgacc gcgaagaaga ccgaggcgcg cgacgtcgcg
     5881 ccgacggccg ccgagccgga ccgctcgctc gaacagagcc tgcggccgca gcgtctctcc
     5941 gacgacgagt ac
//
LOCUS       DASTZR010000465         4042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_521625, whole genome shotgun sequence.
ACCESSION   DASTZR010000465 DASTZR010000000
VERSION     DASTZR010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521625"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VFV20_10415"
     CDS             complement(<1..386)
                     /locus_tag="VFV20_10415"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011190148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEU5288807.1"
                     /translation="MTENTPRPHDSTYGGATAAPPRKLTVPEVVAMKRDGKRITMMTA
                     YDAAFARLVDRAGIDVLLVGDSAGMVMLGYQNTVPVTMDDMVRMAASVSRGATRPLLV
                     GDMPFGSYQTGPQDALRNAARFLKEAG"
     gene            518..1270
                     /locus_tag="VFV20_10420"
     CDS             518..1270
                     /locus_tag="VFV20_10420"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5288808.1"
                     /translation="MTTSTYNATVTARSDLTEALAFFQVRPDDRPAPFAPGQYFTLGL
                     ASGDKLVQRPYSVASSARRIAEGYELFIRLIPGGALTPLLFGTREGDRIWVRGPKGRF
                     TLAPDDERLHLFVATGCGIAPFISMLRTLRDDHATRRIVLLHGVSYARELAYRLFLED
                     LAADPARACTYVPTVSRPAAPENADWHGSMGRVEAVLGGVCERLGIDARDAVAYVCGN
                     PDMTAAVKRVLTDRGFDRLQIHTEDYWPLATR"
     gene            1317..2126
                     /locus_tag="VFV20_10425"
     CDS             1317..2126
                     /locus_tag="VFV20_10425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5288809.1"
                     /translation="MRRRELAGHLQRAEGVMIGTYLDPDPRHPRFSVDASKRIDGVVC
                     LAARHMDLDFLGRALGFGTTRDATPRLLDRIERHYAALGRPTRIAIANGCVPVSTLRL
                     LERRGYAPVDAAPVLLYLYDRARPPAMPMVADLTIERVGPELASLYARTSAESFPERG
                     PDFATVIGRLVRSRRRGLRTFLGRIGGEPAATGMLMDVAPVGGLMNGSVRPAFRGRGI
                     QRALLAHRMRDGWSRGYRLFFGETGNPASAHNMEALGWRKLCDETTWERRA"
     gene            2204..2614
                     /locus_tag="VFV20_10430"
     CDS             2204..2614
                     /locus_tag="VFV20_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008344674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEU5288810.1"
                     /translation="MSEPRQLPYGLRITTALPYDEAVARMKDALKEQGFGVLTEIDVK
                     RTLKEKRDIDFRRYVILGACNPGFAHRALTDEIDIGLLLPCNVVVYEVDEGTVVETID
                     PVATLGRVRNPALEPLGQEVKEALRTALAAVPAR"
     gene            complement(2622..3833)
                     /gene="coaBC"
                     /locus_tag="VFV20_10435"
     CDS             complement(2622..3833)
                     /gene="coaBC"
                     /locus_tag="VFV20_10435"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEU5288811.1"
                     /translation="MGELSGKFVVLGVTGSIAAYKAIELARRFTQAGATVQVVMTRSA
                     TEFVRPITFQALTYRPVEVEMFRIQDERAAGHVAMGREADVVVIAPATAHLLARLANG
                     MADDLIATTVLATGAPVVVAPAMETHMWQNPATQDNVARLRARGVHVVDPESGPLASG
                     DSGPGRLASLDAIEAAVVDAMTPPSSALAGRRVIVTAGPTLEPIDPVRFVSNRSSGKM
                     GYAVACAARDAGAEVTLVSGPTALARPGGVRFIAAETAEELKDTVLALLPQTDVVIMA
                     AAVADYRPRETQVEKIKKRDVGDELVIRMTENPDVLKAVIAARRPGTVVVGFKAETGD
                     ATAEAERMLRDKGLDLVVANDITDAGSGFGSDTDRVTFVSADGVQPLPLLAKTEVARR
                     LVAKLAERFAR"
BASE COUNT          580 a         1460 c         1435 g          567 t
ORIGIN      
        1 ccggcctcct tgaggaagcg cgccgcgttg cgcagcgcgt cctgcggtcc ggtctggtac
       61 gagccgaagg gcatgtcgcc gacgaggagc ggccgcgtgg cgccgcgcga caccgacgcg
      121 gccatgcgca ccatgtcgtc catcgtcacc ggcacggtgt tctggtagcc gagcatgacc
      181 atgcccgcgg aatccccgac gagcaggacg tcgatgccgg cgcggtcgac gaggcgcgcg
      241 aacgccgcgt cgtaggcggt catcatggtg atgcgcttgc cgtcgcgctt catggcgacg
      301 acctctggga cggtcagctt ccgcggcggg gcggcggtgg caccgccgta cgtggagtcg
      361 tggggacgcg gggtgttctc ggtcacgcga cacctccgtc caggccggct ccgcgccggt
      421 cccggcgaat gccatgagtt tagccccgcg ggaccccgtc gaatatgtcc gcccgttttc
      481 cgcggtccgc gtggtccatg ctgcgcggaa cgccctgatg acgacgtcga cgtacaacgc
      541 gacggtcacc gcgcgcagcg atctcaccga agcgctcgcg ttcttccagg tgcgtcccga
      601 cgaccggccg gcgccgttcg cgccggggca gtacttcacg ctcgggctcg cgtccggcga
      661 caagctcgtc cagcggccgt actccgtcgc gagcagcgcc cgcaggatcg ccgagggcta
      721 cgagctcttc atccgtctca tccccggcgg cgcgctgacg ccgctcctct tcggtacgcg
      781 cgaaggcgac cgcatctggg tgcggggccc gaagggacgc ttcacgctcg cgccggacga
      841 cgagcggctg cacctgttcg tcgccacggg atgcggcatc gccccgttca tctcgatgct
      901 gcgcaccctg cgcgacgacc acgcgacgcg gcgcatcgtg ctgctgcacg gcgtgagcta
      961 cgcgcgcgag ctggcctacc gcctgttcct cgaggacctc gcggccgatc cggcccgcgc
     1021 gtgcacgtac gtgcccacgg tctcccgccc cgccgcgccc gagaacgccg actggcacgg
     1081 gagcatggga cgcgtcgagg ccgtgctcgg cggggtgtgc gaacggctcg gcatcgacgc
     1141 tcgcgatgcg gtcgcgtacg tctgtggcaa cccggacatg accgccgcgg tgaagcgggt
     1201 gctcaccgac cggggcttcg accgcctcca gatccacacc gaggattact ggccgctcgc
     1261 gacacgctag cggtctcgcc tcggagcgcc cgtcgtcacg tagagtcggt tcggcgatgc
     1321 gacggcgtga gctcgcgggc cacctgcagc gcgccgaggg cgtgatgatc gggacgtatc
     1381 tcgatcccga tccgcgccac ccgcggttct cggtcgacgc ctcgaagcgg atcgacggcg
     1441 tcgtctgtct cgcggcgcgg cacatggacc tggacttcct cggccgcgcg ctcggcttcg
     1501 gcacgacccg ggacgcgacg ccgcgcctgc tcgaccgcat cgagcgccac tacgcggcac
     1561 tgggccggcc gacgcgcatc gccatcgcga atggctgcgt gcctgtgtcg acgctgcggc
     1621 tgctggagcg ccgcgggtac gcacccgtag acgcagcgcc ggtcctgctc tacctctacg
     1681 accgcgcgcg gccgccggcg atgccgatgg tggcggacct caccatcgag cgcgtcggac
     1741 cggagctggc gtcgctgtac gcgcgcacca gcgccgagag cttccccgag cgcggaccgg
     1801 acttcgcaac ggtcatcggg cggctcgtgc gctcgcgccg ccgcgggctc cgtacgttcc
     1861 tcgggcgcat cggcggcgaa ccggcggcga cgggcatgct catggacgtc gcgccggtcg
     1921 gcgggctcat gaacggcagc gtgcggccgg cgttccgcgg gcgcggtatc cagcgcgcgc
     1981 tcctcgccca ccggatgcgc gacgggtggt cgcgcgggta ccggctgttc ttcggcgaga
     2041 ccggcaaccc cgcgtcggcg cacaacatgg aggcgctcgg gtggcggaag ctctgcgacg
     2101 agacgacctg ggaacgacgt gcctgacact gtcccgacgt gcccgacagc cacgccgcgc
     2161 gcacgcgggt agcctcgaca cgaacgccat ctggaggacc gcgatgagcg aaccacgtca
     2221 gctgccctat ggactgcgca tcaccaccgc gctgccgtac gacgaggcgg tcgctcggat
     2281 gaaggacgcg ctcaaggaac agggcttcgg tgtgctcacg gagatcgacg tgaagcggac
     2341 gctgaaggag aagcgtgaca tcgacttccg ccgctacgtc atcctcggcg cgtgcaatcc
     2401 gggcttcgcg caccgcgcgc tgaccgatga gatcgacatc ggactgctgc tcccatgcaa
     2461 cgtcgtcgtg tacgaggtgg acgaggggac cgtcgtcgag acgatcgatc cggtggcgac
     2521 gctgggacgc gtccgcaatc ccgcgctcga gcccctgggc caggaggtga aggaggcgct
     2581 ccggacggcg ctcgccgccg tccccgcgcg atagaccccg cctagcgggc gaaacgctcc
     2641 gcgagcttcg cgaccagccg ccgcgcgacc tcggtcttcg cgagaagggg cagcggctgc
     2701 acgccgtccg cgctcacgaa cgtcacgcgg tcggtgtcgc tgccgaaccc ggagccggca
     2761 tcggtgatgt cgtttgcgac gaccaggtcc aggcccttgt cgcgcagcat gcgctcggcc
     2821 tccgcggtcg cgtcaccggt ctccgccttg aagccgacga cgacggtccc cggcctgcgc
     2881 gccgcgatga ccgccttcaa cacgtccggg ttctcggtca tgcggatcac gagctcgtcc
     2941 cccacgtcgc gcttcttgat cttctcgacc tgcgtctcgc gcggccggta gtcggcgacg
     3001 gccgccgcca tgatcacgac gtccgtctgc ggcaggagcg cgagcacggt gtccttcagc
     3061 tcctccgcgg tctcggcggc gatgaaacgc acgccgcccg gacgcgcgag ggccgtgggt
     3121 cccgacacga gcgtcacctc ggcgccggcg tcccgcgccg cgcaggcgac ggcgtacccc
     3181 atcttcccgc tcgagcggtt cgacacgaag cggacggggt cgatcggctc gagggtgggc
     3241 ccggccgtca cgatgacgcg ccggccggcg agcgccgacg acggaggggt catcgcgtcc
     3301 acgaccgccg cctcgatcgc gtcgagcgac gcgagccgac ccggaccgga atcgcccgac
     3361 gcgagggggc cggactccgg atccaccacg tggacgccgc gcgcgcggag ccgtgccacg
     3421 ttgtcctgcg tcgcggggtt ctgccacatg tgcgtctcca tcgccggcgc gaccacgacc
     3481 ggcgcgccgg tggccaggac cgtcgtcgcg atcaggtcgt cggccatccc gttcgcgagg
     3541 cgcgcaagga ggtgcgcggt cgccggcgcg atgacgacga cgtcggcctc gcggcccatc
     3601 gccacgtggc cggccgcgcg ctcgtcctgg atgcggaaca tctcgacctc gacgggccgg
     3661 tacgtcagcg cctggaacgt gatgggacgg acgaactccg tcgccgatcg ggtcatgacg
     3721 acctggaccg tcgcgcccgc ctgcgtgaag cgccgggcga gctcgatggc cttgtacgcc
     3781 gcgatcgatc ccgtgacccc cagcacgacg aacttcccgc tcagctcacc catgccccac
     3841 tccgagaagc gacgaacgca tggtgtgcga gcggacgagg gacgacgaca ttgaggagga
     3901 cgagtgcggg cgaaggacga gggacgacat cgaggaagct gactcgggag tgacgcaggg
     3961 gagcggcaac gcaagaacgc gatcgtgagc ggcgacggga ccgagggccc ccgcgcgggg
     4021 agggttcccg tcgcgatgag cg
//
LOCUS       DASTZR010000466         5031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_829162, whole genome shotgun sequence.
ACCESSION   DASTZR010000466 DASTZR010000000
VERSION     DASTZR010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829162"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VFV20_10440"
     CDS             <1..423
                     /locus_tag="VFV20_10440"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEU5288812.1"
                     /translation="APDGRARRPMSAEVPVGDYVKRVPAPLRPTLQAGRRSIKALAPK
                     GTKETAFRAWPIRYSVNGTYVCGIGNYPRWISVYFFGGADLDDPDGILEGTGKGMRHV
                     KLREPSDAERPALKRMVRRAFRAGTAMSRQTVGGAKRP"
     gene            423..854
                     /locus_tag="VFV20_10445"
     CDS             423..854
                     /locus_tag="VFV20_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288813.1"
                     /translation="MRGPALLVALLATACAAVSHGGPVRDHVSFVDALRARGVTVGIS
                     SEAPPQRGLRGHGTRLVISGAGITQQEVDSYNYDPTDLARVGDASRLAEEDAARLRTA
                     LPSGPVHAYRRERVVVLYTGDDAAMKRLLSDVLGAQFAGTT"
     gene            complement(855..2540)
                     /gene="argS"
                     /locus_tag="VFV20_10450"
     CDS             complement(855..2540)
                     /gene="argS"
                     /locus_tag="VFV20_10450"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU5288814.1"
                     /translation="MLPSTRELVGDRLRDALDRAAAAHGWTGTETAAIDVEIPANPAH
                     GDYATSVAMRLAKALRRSPRDIATAVAAEVRREPPIASAEVAGGGFVNVRLDEAWLRQ
                     QVIEIVKSGESYGRSNALAGKKIQVEFVSANPTGPLTVGNARGGPLGDVIANVLAFSG
                     ADVQREYYVEDDTAQVTRFGISLAVRYRQLFGEQVELPADGYQGDYVKDIAAQIKERH
                     GARYRELSLEEQGAAFVPMAMDWMVADAQRVTGKLGIRFDSWYRQSTFIESGYLDETI
                     EALRKTGVIVERDGVIFFETPEAVALRREGDEGWVLVDADGEAKYLATDIAYHRLCLE
                     ERGIDLKLDVWGANTQYHVAQMKLALPVLGLDPSRFEVVLYQYVRFLKEGVLTRMGRR
                     TGQFLLLEDVIDAVGADVTRFFLLQRGADSQLDFDFELAVQQSNDNPVYYVQYAHARI
                     ASIFRTAGERGLTAEDADVQLLAAPGERELIRLCQKFPELLADIQGHRGVHLLTVYAL
                     ELAGAFHGFYRDHRVVDDGNLPLSKARLRLMQAVQLTLRQTLRLIGVSAPESM"
     gene            complement(2555..3787)
                     /locus_tag="VFV20_10455"
     CDS             complement(2555..3787)
                     /locus_tag="VFV20_10455"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5288815.1"
                     /translation="MRTAARLGRRDAFLYIAAATLGSFGLGVTSFYLNFLYRALGFDG
                     LALGVLVGAQAVGVAAGVVVARFVAPGHSRRLVIIGGGVVVGTGIVGILTLSAFALLA
                     ASAALVGLGGIVASSSGIALLADATEIRARSSRFGQQIALGTMAAFSASVLAGLLAAP
                     VAALFGVPENDPLTLRALVALGGIVGAASAIPILFIRDVAVPPAASGHASRILPRFIA
                     IEAAFGLGAGSFIPFTNLFFADRFALPFGAIGLALGAIAVAGSLGALVHGRIVASRLG
                     QIRGIVTVEFASLPFALAAVLTGNVALTVAMLAVRAALMYGAQATWSAYQLSSFSPAE
                     RAAANATLALSWNVAAAIAASVSGAVRGALGPDGFTVNVLVLIVCYAAGATGVYVLFH
                     GREPQGDLAVPAWPTQPD"
     gene            complement(3784..4944)
                     /locus_tag="VFV20_10460"
     CDS             complement(3784..4944)
                     /locus_tag="VFV20_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU5288816.1"
                     /translation="MSEPTYTQPAQPAPRPSGPNAAALLLVGVILGGLSGGAAATVIA
                     GRAPADLVTLSPSPSALPTSAPVTVTPGTDTTVDVVRDLLPAVVTVVNRGQNGQPQSS
                     GSGFVIDAQQGYVATNNHVIENPRDGTRAGTSFDVIFSDNRTVKATLVGRDPATDVAV
                     LRVPAQNLVAAPLADSDKAPVGGRVIAIGSPLGEFQNTVTEGVISAKGRRVQESANVF
                     LEDMIQTDAAINPGNSGGPLIWIAAKQVVGMNTLVQVDPQTETIAQGLGFAVSANTIR
                     DIANELIKNGQIVRGFIGISYLPLTPRQAISLGLPAAAGITVETVVANSPASQAGIKV
                     GDIITKVNDQQIDQQHPLTSIMVKTRPGDKVKLTIIRGGQTQVIELTLGRQS"
BASE COUNT          777 a         1715 c         1845 g          694 t
ORIGIN      
        1 gcgcccgatg gacgggcgcg gcgaccgatg agcgccgagg tgccggtcgg cgactacgtg
       61 aagcgcgtcc ccgcgccgct gcggccgacg ctgcaggccg ggcgacgttc gatcaaggcg
      121 ctcgcgccga agggcacgaa ggagacggcg tttcgcgcgt ggccgatccg gtacagcgtg
      181 aacggcacgt acgtgtgcgg catcggaaac tacccgcgct ggatcagcgt gtacttcttc
      241 ggcggcgccg acctcgacga tccggacggc atcctcgagg gcaccggcaa gggcatgcga
      301 cacgtcaagc tgcgtgagcc gagcgacgcg gagcgtcccg cgctgaagcg gatggtccgc
      361 cgggcgttcc gcgcgggcac ggcgatgtcc cggcagacgg tgggcggcgc gaagcggccc
      421 tgatgcgagg tccggccctg ctggtcgcgc tcctcgccac ggcctgcgcc gccgtgagcc
      481 acggcggtcc ggtccgcgac cacgtcagtt tcgtcgacgc gctacgcgcg cgtggcgtca
      541 cggtcggcat ctcgagcgag gcaccacccc agcgcgggct gcgcggtcac ggcacgcggc
      601 tggtgatcag cggggccggg atcacgcagc aagaagtcga ttcatacaac tacgatccga
      661 cggacctcgc ccgcgtcggc gacgcgtcgc gcctcgcgga ggaggacgcc gcgcggctgc
      721 gcaccgcact tccgtccggg ccggtccacg cataccgccg cgaacgcgtg gtcgtgctgt
      781 acacgggcga cgacgcggcg atgaaacggc tgctcagcga cgtcctcggc gcgcagttcg
      841 cgggcacgac ctagctacat ggactcgggc gcgctcaccc cgatcaggcg cagggtctga
      901 cgcagcgtca gctgcacggc ctgcatgaga cgcaggcgcg ccttggacag cggaaggttg
      961 ccgtcgtcga cgacgcgatg atcgcgatag aagccgtgga acgcaccggc gagctcgagc
     1021 gcgtacacgg tgaggaggtg cacgccgcgg tgcccctgga tgtcggcgag cagctcgggg
     1081 aacttctggc agaggcggat gagctcgcgc tcacccggag cggcgagcag ctgcacgtcg
     1141 gcatcctcgg ccgtgaggcc acgctccccc gcggtgcgga agatgctcgc gatgcgcgcg
     1201 tgcgcgtact gcacgtagta gacggggttg tcgttcgact gctgcacggc gagctcgaag
     1261 tcgaagtcga gctgtgaatc cgcgccgcgc tgcaggagga agaagcgcgt cacgtcggcg
     1321 cccacggcgt caatgacgtc ctcgagcagc aggaactgtc cggtgcggcg gcccatgcgc
     1381 gtgagcacgc cctccttgag gaagcgcacg tactgataga ggacgacctc gaagcgcgac
     1441 ggatcgagcc cgaggaccgg cagcgcgagc ttcatctgcg ccacgtggta ctgggtgttc
     1501 gcgccccaga cgtcgagctt gaggtcgatg ccgcgttcct cgaggcagag gcggtgatac
     1561 gcgatgtcgg tggcgaggta tttcgcctcg ccatcggcgt cgacgagcac ccagccctcg
     1621 tctccctcgc gtcgcagcgc gacggcctca ggagtctcga agaagatcac gccgtcccgc
     1681 tcgacgatga cgcccgtctt ccgcaacgcc tcgatcgtct cgtcgaggta gccggactcg
     1741 atgaaggtcg attggcggta ccagctgtcg aagcggatgc cgagcttgcc ggtgacgcgc
     1801 tgcgcgtcgg cgaccatcca gtccatcgcc atgggcacga acgccgcgcc ctgctcctcg
     1861 agcgagagct cgcggtaccg tgcgccgtgc cgttccttga tctgggcggc gatgtccttc
     1921 acgtagtcgc cctggtagcc gtcggcgggc agctccacct gttcgccgaa gagctgccgg
     1981 tagcgcaccg cgagcgagat cccgaagcgc gtgacctgtg cggtgtcgtc ctcgacgtag
     2041 tactcgcgct ggacgtcggc gccggagaac gcgagcacgt tcgcgatcac gtcaccgagc
     2101 ggaccgccac gcgcattgcc gacggtgagc gggccggtgg ggttcgcgga cacgaactcc
     2161 acctggatct tcttgccggc gagcgcgttc gaacggccgt aggactcacc ggacttcacg
     2221 atctcgatga cctgctggcg cagccacgcc tcgtcgagcc ggacgttcac gaagccgccg
     2281 ccggcgacct ccgccgatgc gatcggcggc tcgcgccgca cctcggccgc gacggcggtc
     2341 gcgatgtcgc gcggcgatct ccgcagcgcc ttggcgagcc gcatcgcgac gctcgtcgca
     2401 tagtcgccgt gcgccggatt cgcggggatc tcgacgtcga tcgccgccgt ttccgtcccg
     2461 gtccagccgt gcgccgcggc ggcgcggtcc agcgcgtcgc gcaggcggtc gccgacgagc
     2521 tcgcgagtcg aggggagcac gcgtcgagtc tcgcctaatc cggctgcgtt ggccacgcgg
     2581 gcacggccag gtcgccctgt ggctcgcgcc cgtggaacag gacgtagacg cccgtcgcgc
     2641 ccgcggcata gcagacgatg agcacgagca cgttcaccgt gaaaccgtcc gggccgagcg
     2701 cgccgcgcac cgcgcccgag acggaggcgg cgatcgcggc tgccacgttc cacgagagcg
     2761 ccagcgtggc gttggcggcg gcacgctcgg cggggctgaa gctcgagagc tggtacgcgg
     2821 accacgtcgc ctgcgcgcca tacatgagcg cggcgcgcac ggcgagcatc gcgaccgtga
     2881 gggcgacgtt tccggtgagc acggccgcga gcgcgaacgg cagcgacgcg aattcgaccg
     2941 taacgatgcc gcggatctgg cccagccgcg acgcgacgat acggccgtgc acgagcgcgc
     3001 cgaggctgcc ggccacggcg atggcgccca gcgcgagtcc gatcgcgccg aagggaagcg
     3061 cgaagcggtc ggcgaagaac aggttcgtga acgggatgaa cgagccggcc ccaagaccga
     3121 aggcggcctc gatcgcgatg aagcgcggca ggatgcggct cgcgtgaccc gacgcggccg
     3181 gcggcacggc gacgtcgcgg atgaagagga tcgggatcgc cgacgccgcg ccgacgatgc
     3241 ctccgagcgc gacgagcgct cggagcgtca gcggatcgtt ctcggggacg ccgaacagag
     3301 cggcgaccgg cgcggcgagc aggcccgcga gcacgctggc gctgaacgcg gccatcgtgc
     3361 cgagggcgat ctgctgtccg aaccgcgagg agcgcgcgcg gatctccgtc gcatcggcga
     3421 ggagcgcgat gcccgagctc gacgcgacga tgccgccgag gccgacgagc gccgccgacg
     3481 cggcgaggag cgcgaacgcc gagagcgtga ggatgccgac gatgccggtg ccgaccacca
     3541 cgccgccgcc gatgatcacc agacggcggg aatgcccggg cgcgacgaac cgcgctacga
     3601 cgacgccggc ggcaacgccg accgcctgcg cgccgacgag caccccgaga gcgagcccgt
     3661 cgaagccgag cgcgcggtag aggaagttca gatagaagga tgtgacaccg agaccgaagg
     3721 accccagcgt cgcggcggcg atgtagagga acgcgtcgcg ccgtccgaga cgcgccgccg
     3781 tcctcaggac tggcggccca gcgtgagctc gatgacctgg gtctgaccgc cgcggatgat
     3841 ggtgagcttc accttatcgc ccggccgcgt cttcaccatg atcgacgtga gcggatgctg
     3901 ctggtcgatc tgctggtcgt tcaccttggt gatgatgtcg cccaccttga tgccggcctg
     3961 cgacgccgga gagttcgcca cgacggtctc gacggtgatg cccgccgcgg ccggaaggcc
     4021 gagagagatc gcctgacgcg gcgtgagcgg cagataggag atgccgatga agccgcggac
     4081 gatctgcccg ttcttgatga gctcgttcgc gatgtcgcgg atcgtgttgg ccgacacggc
     4141 gaagccgagc ccctgcgcga tcgtctcggt ctgcgggtcg acctgcacca gcgtgttcat
     4201 gcccacgacc tgcttcgccg cgatccagat gagcggaccg cccgagttcc cggggttgat
     4261 cgccgcgtcg gtctggatca tgtcctcgag gaagacgttc gcgctctcct ggacacggcg
     4321 tcccttcgcc gagatgacgc cctcggtcac ggtgttctgg aactcaccga gcgggctgcc
     4381 gatggcgatg acgcgaccgc ccaccggcgc cttgtcggaa tcggcgaggg gcgcggcgac
     4441 gaggttctgc gccggcacgc gcaggaccgc cacgtcggtc gcgggatcgc ggccgacgag
     4501 cgtggccttc accgtgcggt tgtcgctgaa gatcacgtcg aacgaggtgc cggcacgcgt
     4561 gccgtcgcgc gggttctcga tgacgtggtt gttcgtcgcg acgtagccct gctgcgcgtc
     4621 gatgacgaag ccggagccgc tggactgcgg ctggccgttc tgcccgcggt tcacgaccgt
     4681 cacgaccgcc gggagcaggt cgcggacgac atccaccgtg gtgtccgtcc cgggcgtgac
     4741 cgtgacggga gcgcttgtcg gaagcgcgct cgggctgggg ctcagcgtga ccagatccgc
     4801 gggcgcgcgg cccgcgatga ccgtcgccgc cgcaccgccg gagagcccgc cgaggatcac
     4861 cccgacgagg aggagcgccg cggcgttcgg accggaggga cgcggcgcgg gctgcgctgg
     4921 ctgggtgtac gtcggctcag acattcatga ccgccctgga gggttacctc cgcgggcgcg
     4981 ccgcggtttc ggtacccggt gtggtcgcct tgcgccggcg cgccgtcggc c
//
LOCUS       DASTZR010000467         5771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_332546, whole genome shotgun sequence.
ACCESSION   DASTZR010000467 DASTZR010000000
VERSION     DASTZR010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332546"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..815
                     /locus_tag="VFV20_10465"
     CDS             45..815
                     /locus_tag="VFV20_10465"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5288817.1"
                     /translation="MHVYVTGASSGIGEAIAREYARRGASLTLVARRRDRLEALAADL
                     SGVRTWVVEHDLANVERVAEPVAEAEAELGPIDVLVNNAGVQIVGATSTTPWEEGEAL
                     LRVDTLAPLKLTLHVLPGMLARGAGTIVNMASMAAIAPTPGMFFYNAAKAALAAGSEG
                     LRGELRGTGVHVVTVYPGPVRTAMDAAAREALMQSRVARMMSPTGDPQVLARLVADAV
                     ESKRPRVIYPRVYAISRHFPSTTRWLLDRLTPKPRIGV"
     gene            complement(874..945)
                     /locus_tag="VFV20_10470"
     tRNA            complement(874..945)
                     /locus_tag="VFV20_10470"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(911..913),aa:Lys,seq:ctt)
     gene            complement(962..1402)
                     /locus_tag="VFV20_10475"
     CDS             complement(962..1402)
                     /locus_tag="VFV20_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013986218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5288818.1"
                     /translation="MKVKDLMTSEPATVGPDDPCARAATLMREEDCGSIPVVRDGALV
                     GIVTDRDITIRAVAAGKDPRSTPVSEVMSADPVTVAPDADAEEASRLMAEFQVRRLPV
                     VADGRLLGILVTAQLARRESDDQVGETIKEISEPASGRASHARG"
     gene            complement(1449..1592)
                     /locus_tag="VFV20_10480"
     CDS             complement(1449..1592)
                     /locus_tag="VFV20_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HEU5288819.1"
                     /translation="MASGYCVKCKASREIKDAKQITMKNGRPATQGACPVCGTKIFKI
                     GAA"
     gene            1760..2875
                     /locus_tag="VFV20_10485"
     CDS             1760..2875
                     /locus_tag="VFV20_10485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HEU5288820.1"
                     /translation="MRAEILSIGTELLLGQILDTNAQYLAQRLAALGIDLLYVSTVGD
                     NLTRCTQTVERAFARSDLVICTGGLGPTEDDLTREAIAAALGEEPVVDAGLERTLREW
                     FAGRGHPMPERNRKQAWLIPSARAIPNPNGTAPGWDVRKDGRRIVSMPGVPREMTHMW
                     EHHVEPTLIAGAVLRWRTLKLLGIGESSVEEALAELVRSTAPTVATYAKNDGVHVRIT
                     DKATDAATADARIAAMERTIRDRLDRYVWGTDDETLGTVIGTALVSRGWRLATAESLS
                     AGDVARTLADAPNATAWLAYGVVMPRADGAALGGALARDADVRLVVPFGETASDIVVT
                     TPEGRSVATARFGSLVEGRRRALLTSLDLLRRALATR"
     gene            complement(2876..4003)
                     /locus_tag="VFV20_10490"
     CDS             complement(2876..4003)
                     /locus_tag="VFV20_10490"
                     /inference="COORDINATES: protein motif:HMM:NF039823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HEU5288821.1"
                     /translation="MKDDRLRDAIRIALRIHRQTAEAPDDYTRLRIRARVLGNLRPRG
                     AGLADHAWTALELLARPAPMIARSFALAALLVAVGMGATVASADTLPDDLLYPVKVAA
                     EGVRLALAGAPEDRAEVELSIAEHRLREAERLAASGRTSDALVAAAVYSQHIAWAAAE
                     LAPRSDSSDLPQQLESRFAVQRDRAQTLAASLANDAKSAPAARILAMIGAPTLAPGVT
                     GPQRVAYTAAGVAEQLVKAAEQAEVHTAAPGAIATPAGAREKETTPAAPPTDARTTIT
                     TTREARPTATVTTQDATATSRPRETSRTREADATPRTRVSDAEPPTQPASSDDASVRR
                     GNEQGARPSSRPDGRSSEVLKVVRKALEEAKAAADKTKQHK"
     gene            complement(4284..>5771)
                     /locus_tag="VFV20_10495"
     CDS             complement(4284..>5771)
                     /locus_tag="VFV20_10495"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEU5288822.1"
                     /translation="DAALAYGKTGSLGDHLSAWADALRGEARVPLTLHADGVALERWL
                     AAISADIDRPAVAGALAVEAGGLAITEPVIGLQLDKVATAATILAAQSLSDRRIDLAV
                     RQIYPAVDDAGFAEAAARARAATTPLSVVVEDRRVNEDAVGLASLLTVERVLAKPNEL
                     PAVPAGAIAPAARYRYTVDIDQTRLAEWVAALGVKLDRPAVSARFTVNKDGALGVVPG
                     VSGIRLDQDAMRTLMQAALLKPAAGAVRELTAPSGADSTAFTTEQAKEWQSKLVRTSS
                     FTTNFPVSASRHANIATGSSQFDGVVIMPGETFSFWSLLGPVTTERGYAYAGAIIQNR
                     SDESVIGGGLCQVSTTIFNAFARLGYDIVERHEHGYLVERYPIGLDAAVFDPGLDFRW
                     KNDTASPVFLWSWVSDTSVTFDVYGLPTGRTVRFSDPIQRNFVNVPADQPADPAFPKG
                     YAIRGRDVVRTRTVTDASGATVHQDTWFSHYAPVWGGPAESMTVH"
BASE COUNT          792 a         2007 c         2100 g          872 t
ORIGIN      
        1 cttcgcagcg aggtcgtccg gccgatccgc tttagcatcc cgcgatgcac gtttacgtca
       61 ctggcgcatc gagtggcata ggcgaggcca tcgcgcgcga gtacgcgcgt cgcggggcgt
      121 cgctgacgct cgttgcgcgc cgccgtgacc ggctcgaggc gcttgcggcc gacctctcgg
      181 gcgtgcgcac gtgggtcgtc gagcacgacc ttgcgaacgt cgagcgtgtc gcggaacctg
      241 tcgccgaggc ggaggcggag ctcggcccga tcgacgtcct cgtcaacaat gccggcgtcc
      301 agatcgtcgg cgcgacgtcg acgacgccgt gggaagaagg tgaggcgctg ttgcgcgtcg
      361 acaccctcgc gccgctgaag ctgacgctcc acgtgctgcc cggcatgctc gcacgcggcg
      421 ccggcacgat cgtcaacatg gcatcgatgg cggcgatcgc gcccacgccc ggcatgttct
      481 tctacaacgc ggcgaaggcg gcgctcgcgg ccggctcaga ggggctgcgt ggagagctcc
      541 gcggcacggg cgtgcacgtg gtcacggtct atccgggtcc ggtgcgcacc gcgatggacg
      601 ccgccgcgcg cgaggcgctg atgcagtcac gcgtcgcccg catgatgtcg cccaccgggg
      661 atccgcaggt gctggcccgg ctggtggcgg acgcggtcga atcgaagcgc ccgcgcgtca
      721 tctatccccg cgtctacgcg atctcacgtc acttcccgag cacgacgcga tggctgctcg
      781 atcgcctcac cccgaagccc cggatcggcg tgtgagcgcc aagaggaatc gcatccggtg
      841 gatgtcttgc gatgtgctga gacatcacac gtgtgagcca ggagggattc gaacccacga
      901 ccaagggatt aagagtcccc tgctctaccg ctgagctact ggcccacctg attttatccc
      961 gtcagccacg cgcatgagaa gcgcgaccgc tcgcgggttc actgatctcc ttgatggtct
     1021 cgccgacctg atcgtcgctc tcgcgtcgcg cgagctgcgc ggtgacgagg atcccgagca
     1081 ggcgcccatc cgccacgacc ggcagcctgc gcacctggaa ctccgccatc agccgcgagg
     1141 cctcttcggc gtcggcgtcg ggcgcgacgg tcaccggatc cgcgctcatc acctcggata
     1201 ccggcgtgga gcggggatcc ttgcccgcgg cgacggcgcg gatggtgatg tcacggtccg
     1261 tcacgatgcc gacgagcgca ccgtcgcgga ccaccgggat ggacccgcag tcctcctcgc
     1321 gcatgagcgt ggccgcccgt gcgcagggat cgtccggacc caccgtcgcg ggctcggacg
     1381 tcatgagatc tttgaccttc atgtgagcac ctcctgccga gacacgagca ggtttggtgc
     1441 tcaggctgtc aggcggcgcc gatcttgaag atcttcgtgc cgcagacggg gcaggcgccc
     1501 tgcgtcgcgg gccggccgtt cttcatggtg atctgcttcg cgtccttgat ctcgcgggag
     1561 gctttgcatt tgacgcaata gccactcgcc actgggcacc tccggtatga gcagtcggcg
     1621 catcgtaccc taggccttcc gaccccctag aaccacaaaa ccccgccttt cggcggggtc
     1681 tcgtggtgag gaagggaagg gttgggattt cttcggttgt tggcagctgg tcgaagcaag
     1741 ttgtgtgcca ggaggcgacg tgcgcgcaga gatcctgtcc atcggcaccg agctcctgct
     1801 ggggcagatc ctcgacacca acgcccagta cctggcgcag cgcctggccg cgctcggcat
     1861 cgacctcctc tatgtatcca ccgtcggcga caacttgacg cggtgcacgc agacggtcga
     1921 gcgagcgttc gctcggtcgg acctcgtcat ctgcaccggc ggcctcggcc cgaccgagga
     1981 cgacctgacg cgggaggcca tcgcggccgc cctcggcgag gagccggtcg tcgacgccgg
     2041 gctcgagcgc acgctgcgcg agtggttcgc cggccgcggc cacccgatgc ccgagcgcaa
     2101 ccgcaagcag gcctggctca tcccctcggc gcgcgccatc cccaatccga acggcaccgc
     2161 gccgggctgg gacgtgcgga aggacggcag gcgcatcgtg tcgatgcccg gcgtgccgcg
     2221 tgagatgacc cacatgtggg agcaccacgt ggagccgacg ctcatcgcgg gcgcggtgct
     2281 ccgctggcgg acgctgaagc tgctcgggat cggcgagtcc tcggtcgagg aggcgctcgc
     2341 ggagctcgtg cggtcgaccg cgccgaccgt cgcgacgtac gcgaagaacg acggggtgca
     2401 tgtgcgcatc accgacaagg ccacggacgc ggcgaccgcg gacgcacgca tcgcggcgat
     2461 ggagcgcacc atccgcgacc ggctggaccg ctacgtgtgg gggaccgacg acgagacgct
     2521 cggcaccgtg atcgggacgg cgctcgtgtc gcgcggctgg cgtctcgcga ccgcggagtc
     2581 gctctcggca ggcgacgtcg cacgcacgct cgccgacgca ccgaacgcca cagcgtggct
     2641 cgcgtacggt gtggtcatgc cccgcgcgga tggcgcggcg ctcggcggcg cgctcgcgcg
     2701 cgacgcggac gtgcggctcg tcgtgccctt cggtgagacc gcgagcgaca tcgtggtcac
     2761 cacgccggag ggtcggtcgg tcgcgacggc gcggttcggg tcgctcgtcg aaggacggcg
     2821 ccgtgcgctg ctgacctcgc tcgatctctt gcgccgcgcc ctcgcgacgc gctgactact
     2881 tgtgctgctt ggtcttgtcg gcggcggcct tggcctcttc gagggccttg cgcacgacct
     2941 tgagcacctc gctgctgcga ccgtcgggcc gcgatgacgg ccgagcgccc tgctcgttgc
     3001 cgcggcggac cgacgcatca tccgaggatg ctggctgcgt aggcggctcc gcatccgaca
     3061 cgcgcgtgcg cggggtcgcg tctgcctccc gcgtgcgcga cgtttcgcgc ggccgcgagg
     3121 tcgcggtcgc gtcctgggtc gtgacggtcg cggtgggccg cgcttcccgc gtcgtcgtga
     3181 tcgtcgtacg ggcgtccgtg gggggcgccg ccggcgtcgt ctccttctcc cgtgcgcccg
     3241 ccggcgtggc gatggccccg ggggccgccg tgtggacctc ggcctgttcc gcggccttca
     3301 cgagctgctc cgcgacgccg gccgcggtat acgcgacgcg ctgcggaccg gtcacgccgg
     3361 gcgcgagggt cggcgcgccg atcatcgcga ggatgcgcgc cgccggtgcg ctcttcgcgt
     3421 cgttcgcgag cgacgcggcg agggtctgcg cgcggtcgcg ctgcacggcg aagcgggatt
     3481 cgagctgctg cggaagatcg gagctgtcgc tgcgcggcgc gagctccgcg gccgcccagg
     3541 cgatgtgctg cgagtacacg gccgcggcga cgagcgcgtc ggatgtgcgg ccgcttgccg
     3601 caaggcgctc ggcctcgcgg aggcggtgct ccgcgatcga gagctcgacc tcggcgcggt
     3661 cctcgggggc gccggcgagc gcgagacgga cgccctcggc cgccaccttc accgggtaga
     3721 ggaggtcgtc cgggagggtg tcggcgctcg cgacggtcgc gcccatcccg accgccacca
     3781 ggagcgcggc gagcgcgaag gagcgcgcga tcatcggcgc cgggcgcgcc aggagctcga
     3841 gcgcggtcca cgcgtggtcg gcgagaccgg cgccacgcgg gcgcaggttg ccgagcaccc
     3901 gcgcgcggat ccggagccgg gtgtagtcat cgggagcctc cgccgtttgg cggtggatgc
     3961 ggagtgcgat ccggatggcg tcgcggaggc ggtcgtcctt cacggagcga aggtcgcgac
     4021 gcccgctggc cacttcgtct agcaggcggt cgagttggtc aggcttgctc ttccccacca
     4081 cggccgtcta acggctcgcc tgtctgcatc gttatccccc gctcgggtga cgccgcggcg
     4141 ggcgagccgc tcccaggaat gggcgcggtg atctccagat gcagcaggca cctcatcaca
     4201 gcatccgcct cccccgcgca catcggccgg gcggggtagc tcgacggcac tggggtcatg
     4261 ccccacacgt acggcgaacg gcgctagtgg acggtcatcg actccgcggg gccgccccag
     4321 acgggagcgt agtgcgagaa ccacgtgtcc tgatggaccg tcgcgccgct cgcgtcggtg
     4381 acggtgcgcg tgcgcacgac gtcacgtccg cggatcgcgt accccttggg gaacgcggga
     4441 tccgccggct ggtccgcggg cacgttcacg aagttgcgct ggatcgggtc gctgaaccgc
     4501 acggtccggc cggtgggcag tccgtagaca tcgaaggtca cgctggtgtc cgacacccac
     4561 gaccacagga agaccggcga ggcggtgtcg ttcttccagc ggaagtccag ccccggatcg
     4621 aagacggcgg cgtcgagccc gatcgggtag cgctcgacga gatacccgtg ctcgtggcgc
     4681 tcgacgatgt cgtagcccag acgcgcgaac gcgttgaaga tcgtcgtcga gacctggcac
     4741 aggccgccgc cgatgacgct ctcgtcggag cggttctgga tgatcgcgcc ggcatacgcg
     4801 tagccgcgct cggtggtcac cggtccgagg agcgaccaga acgagaacgt ctcgccgggc
     4861 atgatcacga cgccgtcgaa ctgcgacgag cccgtcgcga tgttcgcgtg ccgtgacgcg
     4921 ctcaccggga agttcgtcgt gaacgacgac gtcctcacga gcttcgactg ccactccttg
     4981 gcctgctccg tggtgaaggc ggtcgagtcc gcgcccgacg gcgcggtgag ctctcgaacg
     5041 gcgccggcgg ccggcttcag cagcgccgcc tgcatgagcg tccgcatcgc gtcctgatcg
     5101 aggcgtatcc cgctcacgcc gggaacgacg cccagcgcgc cgtccttgtt caccgtgaag
     5161 cgcgcgctca cggcgggccg atcgagcttc acgccgagcg ccgcgaccca ttcggcgagg
     5221 cgcgtctgat cgatgtccac ggtgtagcga tagcgcgcgg cgggcgcgat ggcgccggcg
     5281 gggaccgcgg gcagctcatt cggcttcgcg aggacgcgct cgaccgtgag cagcgacgcc
     5341 agtccgacgg cgtcctcgtt cacgcggcgg tcctcgacga cgacggagag cggcgtcgtc
     5401 gcggccctgg cgcgcgccgc ggcctcggcg aagccggcgt cgtcgacggc cggatagatc
     5461 tgacggacgg cgaggtcgat ccggcggtcg gagaggctct gcgccgcgag gatcgtggct
     5521 gccgtggcca ccttgtccag ctgcaggccg atgaccggct cggtgatggc gaggccgccc
     5581 gcttcgaccg ccagcgcgcc ggcgacggcg ggccgatcga tgtcggctga gatcgcggcg
     5641 agccagcgct cgagcgcgac gccgtccgcg tgaagagtga gcgggacgcg cgcctcgccg
     5701 cggagggcat cggcccacgc gctgaggtgg tcgccgagcg aaccggtctt accgtacgcg
     5761 agcgccgcat c
//
LOCUS       DASTZR010000468         2005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_156291, whole genome shotgun sequence.
ACCESSION   DASTZR010000468 DASTZR010000000
VERSION     DASTZR010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156291"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /gene="tpiA"
                     /locus_tag="VFV20_10500"
     CDS             <1..716
                     /gene="tpiA"
                     /locus_tag="VFV20_10500"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU5288823.1"
                     /translation="PASVASAVDLARAVAASSPAGGAQVGVAPPAIALAAVAEELRGS
                     HVAVYAQDVHWEEKGAFTGQIAASMLAGHAVGSIVGHSEVRRDLGDDDARVARKLARA
                     IASGLRVVLCVGESEAQFLAGETDALIARQIAADTAPLQAAREKAAALVVAYEPIWAI
                     GTGRPATAPHAARAARVIRAALDKAGLPGADVPILYGGSVTAANAAEFAGADGIDGAL
                     VGGASLKSEEFANIVRAFA"
     gene            713..>2005
                     /gene="gpmI"
                     /locus_tag="VFV20_10505"
     CDS             713..>2005
                     /gene="gpmI"
                     /locus_tag="VFV20_10505"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEU5288824.1"
                     /translation="MTARYRPVVLCVLDGWGIAPDGPGNAVTRADTPRLDALLADYPH
                     ARLDASGRAVGLPDGVMGNSEVGHLTMGAGYIQYQELVRINDAIADGTFFDNPALRKA
                     CAAVRDRGTLHLMGLLSTGGVHADLAHLAAMAELAKRAGVRRAVYHCFLDGRDMPPRS
                     ALELLPRVTLPIATIQGRYYAMDRDQRWDRTKRAWDAIVDASAPSAASAEAAVRECYA
                     REGGDELMEPRLVGPGARVEDGDAVVFINFRPDRARQLTRAFMQDDFAGFARPRRPRG
                     LTYVTMTEYKVGLPDVIVAFPPQEVIPMAQLLADRGMRQFHTAETEKYAHVTYFFNGG
                     REAPFDGEDRVLVDSPKVATYDLQPEMSAAGVADTLVKAIRTGAYDFALVNFANPDMV
                     GHTGDIAAALVAMAATDAAVGRIVDAALSAGGCVLVTAD"
BASE COUNT          268 a          727 c          755 g          255 t
ORIGIN      
        1 atccggcgag cgtcgcgtcc gccgtcgacc tcgcgcgcgc ggtcgcggcg tcgtcgccgg
       61 ccggcggcgc ccaggtcggc gtcgcgccgc ccgcgatcgc gctcgccgcc gtggccgagg
      121 agctgcgcgg atcgcacgtg gcggtgtacg cgcaggacgt gcactgggag gagaagggcg
      181 ccttcaccgg tcagatcgcc gcgtcgatgc tcgcgggaca cgcggtcggg tcgatcgtcg
      241 gccactcgga ggtgcgccgc gatctcggcg acgacgatgc gcgggtcgcg aggaagctcg
      301 cgcgcgcgat cgcgagcggc ctgcgcgtgg tcctctgcgt cggtgagagc gaggcgcaat
      361 tcctcgcggg cgagaccgac gcgctcatcg cccgccagat cgcggccgac accgcgccgc
      421 tgcaggcggc gcgcgagaag gccgcggcgc tcgtggtcgc gtacgagccg atctgggcga
      481 tcggcaccgg acggccggcg accgcgcccc acgccgcgcg cgcggcgcgc gtcatcaggg
      541 ccgcgctcga caaggccggc ctcccggggg ccgacgtgcc gatcctctac ggcgggagcg
      601 tgaccgcggc gaacgcggcc gagttcgcgg gcgcggacgg catcgacggc gcgctcgtcg
      661 gcggcgcctc gctgaagagt gaggagttcg ccaacatcgt gcgcgcgttc gcatgacggc
      721 gcgctaccgt ccggtcgtcc tctgcgtgct cgacggctgg ggcatcgcgc ccgacggtcc
      781 gggcaacgcg gtgacgcgcg cggacacgcc gcgtctcgac gcgttgctcg cggattatcc
      841 gcacgccagg ctcgacgcga gcggccgcgc ggtcggcctt cccgacggcg tgatggggaa
      901 cagcgaggtc ggccacctca cgatgggcgc gggctacatc cagtaccagg agctcgtccg
      961 catcaacgac gccatcgccg acgggacgtt cttcgacaac cccgcgctgc ggaaggcgtg
     1021 cgcggcggtg cgcgatcgcg ggacgctcca cctgatgggc ctgctctcga ccggcggcgt
     1081 gcacgcggac ctcgcgcacc tcgccgcgat ggccgagctc gcgaagcgcg ccggcgtgcg
     1141 gcgcgccgtg taccactgct tcctcgacgg ccgcgacatg ccgccgcgga gcgcgctcga
     1201 gctgctgccg cgggtgacgc tccccatcgc gaccatccag ggccggtatt acgcgatgga
     1261 ccgcgaccag cgctgggacc gcacgaagcg cgcgtgggac gccatcgtcg acgcgtcggc
     1321 accatcggcg gccagcgccg aggcggcggt gcgcgagtgc tacgcgaggg agggcggcga
     1381 cgagctgatg gagccgcgtc tcgtcgggcc cggcgcgcgc gtcgaggacg gcgacgcggt
     1441 cgtcttcatc aacttccggc cggaccgcgc gcggcagctc acgcgggcgt tcatgcagga
     1501 cgacttcgcc ggctttgcgc gcccgcgacg gccgcgcggc ctcacctacg tgacgatgac
     1561 cgagtacaag gtcgggctgc cggacgtcat cgtggcgttc ccgcctcagg aggtcatccc
     1621 gatggcgcag ctcctcgccg accgcggcat gcggcagttc cacaccgccg agaccgagaa
     1681 gtacgcgcac gtgacgtact tcttcaatgg cgggcgcgag gcgccgttcg acggcgagga
     1741 ccgcgtgctc gtggactcgc cgaaggtggc cacgtacgac ctgcagcccg agatgagcgc
     1801 ggcaggggtg gctgacacgc tggtgaaggc gatacgcacc ggcgcgtacg acttcgcgct
     1861 cgtgaacttc gccaacccgg acatggtcgg ccacaccggc gacatcgccg cggccctcgt
     1921 ggcgatggcc gcgaccgacg ccgccgtcgg tcgcatcgtc gacgcggcgc tgagcgccgg
     1981 cggatgcgtc ctcgtaaccg ccgac
//
LOCUS       DASTZR010000469         3225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_913497, whole genome shotgun sequence.
ACCESSION   DASTZR010000469 DASTZR010000000
VERSION     DASTZR010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913497"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VFV20_10510"
     CDS             complement(<1..309)
                     /locus_tag="VFV20_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HEU5288825.1"
                     /translation="MTVTTGQLGLARFFEKRGSIPEIVIFAGKGGLGKTTSSAALSHY
                     LSQDLGKRTLCFSTDPQASLSDIFEQNFFGLGVTELKPRLNVVEIDADRRVSEYQKEV
                     K"
     gene            complement(358..588)
                     /locus_tag="VFV20_10515"
     CDS             complement(358..588)
                     /locus_tag="VFV20_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288826.1"
                     /translation="MKGVLYGMASHGHVTATLRTRMYLEHLFMFITLGDMLGIPVLPP
                     YYSLRILPYAVPNIASWKQRVFRERDFTDAIY"
     gene            complement(857..2674)
                     /locus_tag="VFV20_10520"
     CDS             complement(857..2674)
                     /locus_tag="VFV20_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HEU5288827.1"
                     /translation="MLDTPFIFVLIALVAYAIAYFWYGKWFDRSVWKADAGKTTPAHM
                     YLDGVEYFPVSKYVLWGFQFKSVAALGPILGPFIAIQYGWLPALIWIVLGNFFIGWLQ
                     DYGAVMVSVRNQGRSFGPISYEFTGAAGRNTLLWFVLLYLLIISATFIFFIATFWNIF
                     PGVFWATVGVFAAGVIAGRLIYKVRMNILGVSGIALLIVLATLYLSTLVQWPQKDFLG
                     VWTIPFWAAVCLVFLYAGAILPLPSFIQPVNFVAFFPAFGAVLLIIVGALLSPVTNIH
                     LTQPAWKSFFPADTVGPLWPVMFVAIACGAISGWHSLVGSSSTSKQLDIETDAHPVGA
                     GAMLSEGLLGLASLSAYMIVSPQIIQQGNVGAWVFGATQFTQGYLGFLGEVGLKAYFG
                     MALVIFAITVQALVTRFWRLVAAEVSSGTSLSWFGERHIATVLGLVIPWVLAVTGSWI
                     NLWLYFGGANQLLAALALWLISIHFVRVKAPSRFTLIPAVFMTITTIAALAYQTLIFT
                     RAGLGLFGMTLFKVSDKGLELFLNTPAAPLVQAGQWIALPEAKTFAYLFNLVFAVVGL
                     VLFLLGIRMSLYVWDGYRRFSGGGAPAPRPAPAATGGED"
     gene            complement(2797..3126)
                     /locus_tag="VFV20_10525"
     CDS             complement(2797..3126)
                     /locus_tag="VFV20_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288828.1"
                     /translation="MKQVEILGLYGATLRPCSVCDLTDLGSKLQREQAAEATDAARTD
                     SDLAYRIAAAAFGRYGDAVRVRLTPLDSPRGLWLTLRHRLGGGLQVLVNGRKTAPEPE
                     KVVAALA"
BASE COUNT          599 a         1024 c         1109 g          493 t
ORIGIN      
        1 cttgacctct ttctggtact cggacacgcg gcggtcggcg tcgatctcga cgacgttcag
       61 ccgcggcttg agctcggtga cgccgaggcc gaagaagttc tgctcgaaga tgtcggagag
      121 cgaggcctgc ggatcggtgg agaagcagag cgtgcgcttc cccagatcct gcgagaggta
      181 gtgcgagagc gccgcggagc tcgtcgtctt cccgaggccg cccttgccgg cgaagatgac
      241 gatctcggga atgctgccgc gcttctcgaa gaaccgcgcg agcccgagct ggcccgtcgt
      301 gacggtcatc gcgctactcc cctcacctgc tttgtcgtct cgaggcgacc gagcggccta
      361 gtagatcgcg tcggtgaagt cgcgctcgcg gaagacgcgc tgcttccacg atgcgatgtt
      421 cgggaccgcg tacggaagga tgcgcagcga gtagtacggc ggcaggaccg ggatccccag
      481 catgtcgccg agcgtgatga acatgaacag gtgctcgagg tacatgcgcg tgcggagcgt
      541 cgcggtcacg tgaccgtggc tcgccatccc gtagaggacg cccttcatcg ccgtgccgaa
      601 gccggcaccc ttcttctcgt cgggcttctc gggcttgtcc ttctcaggca tatgacgcct
      661 cctcgtggac gctgcgtgcg agtctagcca ttctgggtac ccgctctccg ctcctctccc
      721 ggtacccggg cctccgctcc cttctctgag gtaccgggtc caccgctccc tctttgggta
      781 cccgctctcc gctccctaat gggagcggag acgggtaccc tcgcgccgct ctctaatgag
      841 agcggcgttg cgtgccttaa tcctcgccgc cggtggcggc gggtgcgggt cgcggtgcgg
      901 gcgcgccacc accgctgaag cggcgatagc cgtcccacac gtacagggac atgcggatgc
      961 cgagcaggaa cagcacgagt ccgacgaccg cgaacacgag attgaagagg tacgcgaagg
     1021 tcttcgcctc ggggagtgcg atccactgcc ccgcctggac gagcggtgcg gcaggcgtgt
     1081 tcaggaagag ctcgaggccc ttgtccgaga ccttgaacag cgtcatgccg aagagaccga
     1141 ggcccgccct ggtgaagatg agcgtctgat acgcgagtgc ggcgatcgtc gtgatcgtca
     1201 tgaagaccgc ggggatgagc gtgaaccggc tcggagcctt cacgcgcacg aagtggatcg
     1261 agatgagcca cagcgcgagc gcggcgagga gctggttcgc accaccgaag tagagccaca
     1321 ggttgatcca cgatccggtg acggcgagga cccacgggat gacgaggccg aggacggtcg
     1381 cgatgtgccg ctcgccgaac cacgagaggc tcgtgcccga ggacacctcc gccgcgacga
     1441 gccgccagaa ccgtgtgacc agcgcctgca ccgtgatcgc gaagatcacg agcgccatgc
     1501 cgaagtaggc cttgaggccc acctcaccga ggaatccgag atagccctgc gtgaattgcg
     1561 tcgcgccgaa gacccaggcg ccgacgttgc cctgctggat gatctgcggc gagacgatca
     1621 tgtacgccga gagcgatgcg aggccgagga gaccctcgga gagcatcgcg cccgcgccga
     1681 ccgggtgcgc gtcggtctcg atgtcgagct gcttgctcgt tgacgagcta ccgacgagcg
     1741 agtgccagcc ggagatcgca ccgcaggcga tcgcgacgaa catcaccggc cagagcggtc
     1801 cgaccgtgtc cgccgggaag aacgacttcc acgccggctg ggtcaggtgg atgttcgtga
     1861 ccggcgagag cagtgcgccg acgatgatga ggagcaccgc accgaacgcc gggaagaacg
     1921 cgacgaagtt gacgggctgg atgaagctgg gcagcggcag gatcgcgccc gcgtagagga
     1981 agacgagaca gaccgcggcc cagaacggga tcgtccagac gccgaggaag tccttctgcg
     2041 gccactggac gagcgtcgag agatagagcg tggcgaggac gatgagcagc gcgatgccgc
     2101 tcacccccag gatgttcatg cggaccttgt agatgaggcg accggcgatc acgcccgcgg
     2161 cgaacacgcc gaccgtcgcc cagaacacgc cggggaagat gttccagaag gtcgcgatga
     2221 agaagatgaa ggtcgccgag atgatgagga ggtacagcag cacgaaccag agcagcgtgt
     2281 tccggcctgc cgcaccggtg aactcgtagc tgatgggccc gaacgagcgt ccctggttgc
     2341 gcacgctcac catgaccgcg ccgtagtcct ggagccagcc gatgaagaag ttgccgagca
     2401 cgatccagat gagcgcgggg agccatccgt actggatggc gatgaacggc ccgaggatcg
     2461 gtccgagcgc cgcgacgctc ttgaactgga agccccagag gacgtacttc gagaccggga
     2521 agtactccac gccgtcgagg tacatgtgcg ccggcgtcgt cttccccgcg tcggccttcc
     2581 agacgctgcg gtcgaaccac ttgccgtacc agaagtacgc gatggcgtat gcgacgagtg
     2641 cgataagaac gaagatgaat ggggtatcca gcactacgca tccctcctaa cgaaggtcgc
     2701 ttcgacggcc gaatacgtcg tcccggctgc gctcaccccc tcccgacaag acgcgccaga
     2761 ctgacgggct ggattatggc gaccccaggc aaacgattaa gcgagcgcgg cgaccacttt
     2821 ctcaggctcg ggagccgttt ttcggccatt tacgaggact tgcaggcccc cgccgaggcg
     2881 atggcgaagc gtcagccaga ggccgcgggg cgaatccagg ggcgtgagcc gcacccggac
     2941 cgcgtcgccg taccggccga aggccgcggc ggcgatccgg taggcgaggt cggaatcggt
     3001 gcgcgctgca tcggtcgcct cggcggcctg ctcgcgctgc agcttgctgc cgaggtcggt
     3061 gaggtcgcac accgagcacg gacggagcgt cgctccgtag aggccgagga tctcgacctg
     3121 cttcacggcg cggaatgcta cgccgccgca tcgggcgcgg ataggctcgc cgtgatgcgc
     3181 gcagcgctct tactcgcggg cgcgaccctg accgcgtgcg gtgtg
//
LOCUS       DASTZR010000470         2520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_332598, whole genome shotgun sequence.
ACCESSION   DASTZR010000470 DASTZR010000000
VERSION     DASTZR010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332598"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1298
                     /locus_tag="VFV20_10530"
     CDS             <1..1298
                     /locus_tag="VFV20_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020517231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEU5288829.1"
                     /translation="TPDAVVLPNDTADVAALARFCHEHSVPIVPRGAGTGLSGGAIPV
                     EGGVVVSFARMARILDIDAPNRRAVVQPGLINLHLSTAVAPHGLYFVPDPSSQKACTL
                     GGNVAENSGGPHTLAYGVTTNHVTGLEIVLSDGTIVRLGGKAEDAEGYDLIGAFVGSE
                     GTLGLVTEITVKLTALPEDKRTILAAFPTMDQASESVSAIIAAGVVPAAIEMMDNLAT
                     QAVEAAVGAGYPLDAGGILLVEVDGLRDGLDDAAVRIESICREHGATTLRRAANALER
                     EKLWAGRKGAFAAMGRLAPAYYVQDGVIPRTRLPEVLRAVEEIGARYGVRVANVFHAG
                     DGNLHPLVLFADASELERVHEAGREILTACVEAGGSITGEHGVGMEKNCYMPLQFAPA
                     DLAAMRRLKTAFDPAGLANPGKIFPTPGRCREFQLAAVR"
     gene            1301..2452
                     /locus_tag="VFV20_10535"
     CDS             1301..2452
                     /locus_tag="VFV20_10535"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEU5288830.1"
                     /translation="MATAPLRPAGADELARALRDASEAHRTVRVRGAGTKDYLGDLVP
                     TDTTIETTALTGIVAHVPADLTVTVRAGTSLADLRRALGTAGQMLALDPPHADATVGG
                     IVAANGSGFWRARYGGVRDQLIGTTFALTDGTVAHSGGRVVKNVAGYDLNKLLIGSLG
                     TLAVVTECTFKVLPVPRSTAGTVARFRRPADAFAAADAMTRTSARPAALVAHGTARDA
                     WELYVQADGAPEAVERTLTLAAEAAGTAASDRVEIAGALAPLRELPSQADGAIVRASL
                     PPAAQSSFADVAMRLDGFTRLVADAASGIVRVHVRGLDDDVVAGTDALLAAARVCGGS
                     ARVERRAAHLRARIGAWGDTDLPGRFLMRRLKEAFDPAGVLEPGRGPVR"
BASE COUNT          323 a          966 c          906 g          325 t
ORIGIN      
        1 cgacgcccga cgccgtcgtg ctgccgaacg acacggccga cgtcgcggcg ctcgcgcgtt
       61 tctgtcacga gcacagcgtg ccgatcgtgc cgcgcggcgc cggcacgggc ctctccggtg
      121 gcgcgatccc cgtcgagggc ggcgtcgtcg tgtcgttcgc gcgcatggcg cgcatcctcg
      181 acatcgacgc gccgaatcgg cgcgccgtcg tgcagcccgg cctcatcaat ctgcacctct
      241 cgaccgccgt cgcgccgcac ggcctgtact tcgtgcccga tccgtcgagc cagaaggcct
      301 gcacgctcgg cggcaacgtc gctgagaaca gcggcggacc gcacacgctc gcgtatggcg
      361 tgacgaccaa tcacgtgacg ggactcgaga tcgtgctctc cgacggcacc atcgtccggc
      421 tcggcgggaa ggccgaggac gccgagggct atgacctcat cggcgcgttc gtcgggtccg
      481 agggcacgct cgggctcgtg accgagatca ccgtgaagct cacggcgctc cccgaggaca
      541 agcgcaccat cctcgccgcg ttcccgacga tggaccaggc atccgagtcc gtctcggcga
      601 tcatcgccgc gggggtcgtc ccggccgcga tcgagatgat ggacaacctc gcgacgcagg
      661 cggtcgaggc cgcggtcggc gccgggtacc cgctcgatgc cggcgggatc ctgctggtcg
      721 aggtcgacgg gctgcgcgac ggcctcgacg acgccgccgt gcgcatcgag tcgatctgcc
      781 gcgagcacgg cgccacgacg ctgcggcgtg ccgcgaacgc cctcgagcgc gagaagctct
      841 gggccgggcg gaagggcgcc ttcgcggcga tgggccgcct cgcgcccgcg tattacgtcc
      901 aggacggcgt gatcccgcgc acccggctgc ccgaggtgct gcgcgcggtc gaggagatcg
      961 gcgcgcgcta cggcgtccgg gtcgcgaacg tgttccacgc cggcgacggc aacctccatc
     1021 cgctcgtcct gttcgccgac gcctccgagc tggagcgcgt ccacgaggcg ggccgcgaga
     1081 tcctcaccgc gtgcgtcgag gccggcgggt ccatcactgg cgagcatggg gtcggcatgg
     1141 agaagaactg ctacatgccg ctgcagttcg cgccggcgga cctcgcggcg atgcggcgcc
     1201 tcaagaccgc gttcgatccg gcaggactcg cgaacccggg caagatcttc cccacaccgg
     1261 gccgctgccg cgagttccag ctcgcagccg tgcgttgacc gtggccacgg ccccgctgcg
     1321 tccggccggc gccgacgagc tcgcgcgcgc gctgcgcgac gcgagcgagg cgcaccgcac
     1381 cgtgcgcgtg cgcggcgcgg gcaccaagga ctacctcggc gacctcgtgc cgacggacac
     1441 cacgatcgag acgacggccc tcaccggcat cgtcgcgcac gtgcccgcgg acctcaccgt
     1501 cacggtccgc gccggcacga gcctcgccga tctccgccgc gcgctcggca ccgcgggaca
     1561 gatgctcgct ctcgatccgc cgcacgcgga cgcgaccgtc ggcggcatcg tcgcggccaa
     1621 cgggagcggg ttctggcgcg cgcgttacgg cggcgtacgc gaccagctca tcggcacgac
     1681 cttcgcgctc accgacggca ccgtcgcgca ctccggcggc cgcgtggtga agaacgtcgc
     1741 gggctacgac ctgaacaagc tcctcatcgg ctcgctcggg acgctcgcgg tcgtcaccga
     1801 atgcacgttc aaggtgctgc cggtcccgcg gtccaccgcc ggcaccgtcg cgcgcttccg
     1861 ccgcccggcc gacgcgttcg ccgcagcgga cgcgatgacg cggacgtccg cgcggccggc
     1921 cgcgctcgtc gcgcacggga cggcgcgcga cgcatgggag ctgtacgtgc aggccgacgg
     1981 cgcgcccgag gcggtcgagc gcaccctcac gctcgcggcc gaggcggccg gcaccgcggc
     2041 cagcgaccgc gtcgagatcg ccggggcgct cgcgccgctg cgcgagcttc cgtcgcaggc
     2101 ggatggcgcg atcgtgcgcg cctccctccc gccggcggcg cagtcctcgt tcgccgacgt
     2161 cgcgatgcgg ctcgacggct tcacgcggct cgtcgccgac gcggcgtccg gcatcgtgcg
     2221 ggtgcacgtg cgcggcctcg acgacgacgt ggtcgcgggc accgacgcgc tgctcgccgc
     2281 ggcgcgcgtc tgcggcggca gcgcgcgtgt cgagcggcgc gccgcgcacc tgcgcgcgcg
     2341 catcggcgcc tggggcgaca ccgacctgcc tggacgcttc ctcatgcggc gcctcaagga
     2401 ggcgttcgac ccggcgggcg tgctcgagcc gggccgcgga ccggtgcgat gaccacctat
     2461 ccgagcgcga acgacgcgcc gtcccgcgag ttgctggacc gctgcgtcca ctgcggcttc
//
LOCUS       DASTZR010000471         2939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1018024, whole genome shotgun sequence.
ACCESSION   DASTZR010000471 DASTZR010000000
VERSION     DASTZR010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018024"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..998)
                     /locus_tag="VFV20_10540"
     CDS             complement(108..998)
                     /locus_tag="VFV20_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HEU5288831.1"
                     /translation="MIPASALDRHSFSTLRPVVLVLDAEPDMAVLRKHYGDDVRPEPL
                     GPRFALLPPREPYVDLASVAAVTRIAERLRAEGGCPWDREQTHESLRPHLLEEAYEAL
                     EALDSGDLDRLRDELGDLLFQIAIHAQLAHEEGAFDISDVARRLGEKLVRRHPHVFAA
                     TEISGDLLAQWERIKREEREAGGNADESILSGVPKDLPALFAAERLQERSARVGVVHE
                     PIDFPLDIDDPAFLGDLLFDLVAAARTKGFDAETALREANERFAAHVARIEERARGEG
                     RALESYAPEEMRRMWEESLA"
     gene            complement(1051..2415)
                     /locus_tag="VFV20_10545"
     CDS             complement(1051..2415)
                     /locus_tag="VFV20_10545"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU5288832.1"
                     /translation="MARRIPLPRLTQRQRMARWQRERRQQTLIVTVFSAILFFVVGLV
                     AWAATDRYYNENLKPAVTMDGRAWAMRDYIRERNFQYVRFYMDYGVPPGYENDQQILQ
                     QKATYDSVALDAIIEHAILDQNAKKDGVVVTQQQIDERYALDYGEYHARHILIAPKPE
                     GNAADATQKADAAALAKAKDIQAQLKAAPNDQELWNKLAKDNSDDPGSKDSGGDLGFV
                     GHGQFVKEFEDAVHATKVGDITEPVKSQYGYHVIQVMELRQPAESQFVQRAASYGYSV
                     ADVKAHVRYDILKDTYAEKAKAASVQSPTEQVRLAWIAVAAPKITGGDFQAFTDQVKK
                     VTEIQKAIDDGTDFAEVVKKYTEDSATQEKGGDLGWFARGMITKLDIEEDVFSLPVGK
                     VSAQKSDAQQTVWYKVLEKDPARALDDDQKTKIANGAYDYWYQHQKKAHHIQKLVPGH
                     ELDT"
     gene            complement(2432..>2939)
                     /locus_tag="VFV20_10550"
     CDS             complement(2432..>2939)
                     /locus_tag="VFV20_10550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5288833.1"
                     /translation="QFDPRKNMDGLVGAFARAAERHPDLRLVVAGELGHLAGFLHAAL
                     ERGHAPRDRVVLAGLVTDDELAALYAAAECLLHPALLEGFGLTPLEALAAGTPVVGYR
                     AGAVEEVVGDAGMLVDDGRPDELGEALLAVLGDDALRARLRERARPRAALFSWDRAAR
                     DTLSVYRG"
BASE COUNT          396 a         1020 c         1028 g          495 t
ORIGIN      
        1 gaacgagatg cgcgccgagc cctcggcgaa cttcgagacg ccgatctcga tggtgatcgg
       61 ccgcagcgcc tccgccgcgc gtccgtcggg acgcttgaag ggcttcgtca tgcgaggctc
      121 tcctcccaca tccgccgcat ctcctcggga gcgtacgact ccagcgctcg gccctccccg
      181 cgcgcgcgct cctcgatgcg cgcgacgtgc gccgcgaatc gctcgttcgc ctcgcgcagg
      241 gccgtctccg catcgaaccc cttcgtgcgc gccgcggcga cgagatcgaa gaggaggtcg
      301 ccgaggaacg cgggatcgtc gatgtccagg ggaaagtcga tcggctcgtg cacgacgccg
      361 acgcgcgcgc tccgttcctg cagccgctcc gccgcgaaga gcgcgggcag gtccttcggg
      421 acaccggaca ggatgctctc gtccgcgttg ccgccggcct cgcgctcctc gcgcttgatg
      481 cgctcccact gcgcgagcag atcgccggag atctcggtcg cggcgaagac gtgcggatgg
      541 cgccgcacca gcttctcgcc gaggcggcgc gcgacgtcgg agatgtcgaa ggcgccctcc
      601 tcgtgcgcga gctgcgcgtg gatcgcgatc tggaagagca gatcgccgag ctcgtcgcgc
      661 agccggtcga ggtcgcccga gtcgagcgcc tccagcgcct cgtacgcctc ctcgaggagg
      721 tgcggccgca ggctctcgtg cgtctgttcc cgatcccatg ggcagccgcc ctccgcgcgc
      781 agccgctcgg cgatgcgcgt gaccgcggcg acggacgcga ggtctacgta cggctcgcgc
      841 ggcggcagga gcgcgaagcg cgggccgagg ggctcggggc ggacgtcgtc gccgtagtgc
      901 ttgcgcagca cggccatgtc gggctcggcg tcgagcacga gcaccaccgg acgcagcgtc
      961 gagaacgagt gccggtcgag cgcgctcgcg gggatcacgt cgacgcctcc gggaaaacgc
     1021 gcgaggagtc gctcgagggc cgcgccgagc tcaggtgtcg agctcgtggc ctgggaccag
     1081 cttctggatg tggtgcgcct tcttctggtg ttgataccag tagtcgtagg cgccgttcgc
     1141 gatcttcgtc ttctgatcgt cgtcgagcgc ccgggcgggg tccttctcga ggaccttgta
     1201 ccagaccgtc tgctgcgcgt cgctcttctg cgcgctcacc ttgccgaccg gaagcgagaa
     1261 cacgtcctcc tcgatgtcga gcttggtgat catgccgcgc gcgaaccatc cgaggtcgcc
     1321 gcccttctcc tgggtcgcgg agtcctcggt gtacttcttg acgacctcgg cgaagtccgt
     1381 cccgtcgtcg atcgccttct ggatctcggt gaccttcttg acctggtcgg tgaaggcctg
     1441 gaagtcgccg ccggtgatct tgggcgcggc gacggcgatc cacgcgaggc gcacctgttc
     1501 cgtcggcgac tgcaccgatg cggccttcgc cttctcggcg tacgtgtcct tgaggatgtc
     1561 gtagcgcacg tgcgccttca cgtctgcgac ggagtagccg taggacgcgg cgcgctgcac
     1621 gaactggctc tcagcgggct gccgcagctc catgacctgg atgacgtggt agccgtactg
     1681 gctcttcacc ggctcggtga tgtcaccgac cttcgtcgcg tgcacggcgt cctcgaactc
     1741 cttcacgaac tggccgtggc cgacgaagcc aaggtcgccg cccgaatcct tcgaacccgg
     1801 gtcgtcggag ttgtccttcg cgagcttgtt ccagagctcc tgatcgttcg gcgcggcctt
     1861 cagctgcgcc tggatgtcct ttgccttcgc gagcgcggcg gcgtcggcct tctgcgtcgc
     1921 gtcggccgcg ttgccctcgg gcttgggcgc gatgaggatg tggcgcgcgt gatactcgcc
     1981 gtagtcgagg gcgtagcgct cgtcgatctg ctgctgcgtg acgacgacgc cgtccttctt
     2041 cgcgttctgg tcgaggatcg cgtgttcgat gatcgcgtcg agcgcgaccg agtcgtacgt
     2101 cgccttctgc tgcaggatct gctggtcgtt ctcgtagccg ggcggcacgc cgtagtccat
     2161 gtagaaccgc acgtactgga agttgcgctc gcggatgtag tcgcgcatcg cccaggcacg
     2221 gccgtccatg gtgacggccg gcttcaggtt ctcgttgtag tagcggtcgg tcgccgccca
     2281 ggcgacgagg cccacgacaa agaagaggat cgccgagaag accgtgacga tgagcgtctg
     2341 ctggcggcgc tcgcgctgcc accgcgccat gcgctggcgc tgggtgagcc gcggaagtgg
     2401 gatacgtcgt gccacgggat tcccagtcta actaaccgcg gtacacggaa agggtgtcac
     2461 gggccgcgcg gtcccacgag aagagcgcgg cgcggggccg ggcacgttcg cgcagccgcg
     2521 cacgcagcgc gtcgtcgcca agcaccgcga ggagcgcctc gccaagctcg tccggcctgc
     2581 cgtcgtccac gagcatgccc gcgtctccca cgacctcctc gaccgccccg gcgcggtatc
     2641 ccacgaccgg cgtgcctgcc gcgagcgcct cgagcggcgt gagaccgaag ccctcgagca
     2701 gggccgggtg gaggaggcac tcggccgccg cgtacagcgc ggcgagctcg tcgtcggtca
     2761 cgaggcccgc gagcacgacg cggtcgcgcg gcgcatggcc gcgctccagc gccgcgtgca
     2821 ggaaccccgc gaggtggccg agttcgccgg ccacgacgag ccgcaggtcg gggtggcgct
     2881 ccgccgcccg agcgaacgcg ccgacgagcc cgtccatgtt cttccgggga tcgaactgg
//
LOCUS       DASTZR010000472         3291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1298841, whole genome shotgun sequence.
ACCESSION   DASTZR010000472 DASTZR010000000
VERSION     DASTZR010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298841"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="VFV20_10555"
     CDS             <1..1110
                     /locus_tag="VFV20_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEU5288834.1"
                     /translation="EGSVSRARVTDLFTFQGLAREPVDEVRAGDIVAVAGVADVGIGD
                     TIADPDDPRPLPPIKVEEPTLKMTFAVNTSPFAGREGQHVTSRKLRERLYAELERDVA
                     LRVSDTESPDTLTVSGRGELHLAILIENLRREGYELQVSRPEVIEHYENGVRLEPFEQ
                     LEIEVSQEALGAVVELAGKRRGELLDMKYKDDGTAHCVYKIPTRGLLGFRQAFLTNTR
                     GQGVMNTLFAGYGPYAGDIDSRNFGSLIAWEDGTTTQFGLAQAQERGTLFIGPGVDVY
                     EGMVVGEHIRDRDLEVNVVRRKHLTNMRSSNSDIAVKLDGMRELSLDDAIEFLADDEL
                     LEVTPKTFRIRKKVLTKQDRDRIIGQRKKAAGVAD"
     gene            1197..1958
                     /locus_tag="VFV20_10560"
     CDS             1197..1958
                     /locus_tag="VFV20_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013286182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5288835.1"
                     /translation="MFRHLAKVLPPVGVAVGRFDRRGYDVPLDDQADDALAFVSELEV
                     RPDIDASHIGLWGFSQGAWVAPLAASRSKKVAFLVLLASTGVTPAEQMQYGTAKHVRM
                     AGYGEDAAGRVVELRQLVDEWRRGRVPIERAQAELDRARREPWFEQAYLPERLSAAKA
                     WPDMDFEPAEVFAKVTVPVLLFYGEDDEWQPIDASVAAWRRAAERAGNADLTIVRLAG
                     TGHAPTIGDLETVDAVAPEYERALLEWLRRVARLG"
     gene            complement(1950..3008)
                     /locus_tag="VFV20_10565"
     CDS             complement(1950..3008)
                     /locus_tag="VFV20_10565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5288836.1"
                     /translation="MEIGLYTFGELTPSAGGTTVSPARRLRDLLEEIELADRLGLDVF
                     GVGEHHRPDFAVSAPAVVLAAAAARTERIRLTSAVSVISSDDPVRVFQDFATLDLLSG
                     GRAEIMAGRGSFIESFPLFGHDLKDYDALFAEKLGLLLRVREDTHVTWSGTYRAPLAG
                     QGVYPRPVQDPLPVWIAVGGNAESAVRAGSLGLPMALAIIGGMPERFAPLAQIHREAA
                     RSAGHVPLPRLSINSHGYVADTADEAAETTYGPYKLMMDRIGRERGWPPMSRADFDFS
                     RSLRGANFIGDPEDVIQKILFQHEIFHHDRFLLQMTVGSIPHEKIMRSIELFATKVAP
                     AVREEVARRAKAGAASSA"
     gene            3046..>3291
                     /locus_tag="VFV20_10570"
     CDS             3046..>3291
                     /locus_tag="VFV20_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288837.1"
                     /translation="MTGTMRAATSTPLTEALWRKRFRAARIMFPSWARDDADHLVYTT
                     NSSGKFEVHTWDRRNDTHRQLTDRPEGTGYRVSSRLEP"
BASE COUNT          530 a         1125 c         1186 g          450 t
ORIGIN      
        1 gagggcagcg tgagccgcgc acgcgtcacg gacctcttca cgttccaggg gctggcgcgg
       61 gaacccgtcg acgaggtgcg cgccggcgac atcgtcgcgg tcgcgggcgt ggccgacgtc
      121 ggcatcggcg acacgatcgc cgacccggac gatccgcggc cgctcccgcc catcaaggtc
      181 gaggagccga cgctgaagat gacgttcgcc gtgaacacca gccccttcgc cggacgcgag
      241 ggacagcacg tcaccagccg caagctccgc gaacgcctct acgccgagct ggagcgcgac
      301 gtcgcccttc gcgtctccga caccgagagc ccggacacgc tgacggtcag cggccgcggc
      361 gagctccacc tcgcgatcct catcgagaac ctgcgtcgcg agggctacga gctgcaggtg
      421 tcccgcccgg aggtcatcga gcactacgag aacggcgtcc ggctcgagcc cttcgagcag
      481 ctcgagatcg aggtgtcgca ggaggcgctc ggcgcagtcg ttgagcttgc ggggaagcgc
      541 cgcggcgagc tgctcgacat gaagtacaag gacgacggga cggcgcactg cgtctacaag
      601 atccccacgc gcggcctgct cgggttccgg caggcgttcc tcaccaacac gcgcggccag
      661 ggcgtgatga acacgctctt cgccggctac ggcccgtatg cgggcgacat cgattcgcgc
      721 aacttcggct cgctcatcgc gtgggaggac ggcacgacga cccagttcgg gctggcgcag
      781 gcccaggagc gtggcacgct cttcatcggg ccgggcgtgg acgtgtacga aggcatggtc
      841 gtgggggagc acatccgcga ccgcgacctc gaggtgaacg tcgtccgccg gaagcacctc
      901 acgaacatgc gcagcagcaa ctccgacatc gcggtgaagc tcgacggcat gcgcgagctt
      961 tcgctggacg acgcgatcga gttcctcgcc gacgacgagc tcctcgaggt cacgccgaag
     1021 accttccgca tccgcaagaa ggtgctcacc aagcaggacc gcgatcgcat catcggtcag
     1081 cgaaagaagg ccgccggggt cgccgactga gagtcctcga cctcccggac ggtcgcggcc
     1141 ggcatcccgc gatcgtctgc ctgcatggcg cgagcgacag ctcgcgcgac cacttcttgt
     1201 tccggcatct cgcgaaggtc ctgccgccgg tgggcgtcgc cgtcggacgg ttcgaccggc
     1261 gcggctacga cgtgccgctc gatgaccagg ccgatgacgc gctcgccttc gtgtccgagc
     1321 tcgaggtgcg tcccgacatc gacgcgtcgc acatcgggct gtggggattc agccagggcg
     1381 cgtgggtggc gccgctcgcc gcgtcccggt cgaagaaggt cgcgttcctc gtgctcctcg
     1441 cttcgacggg cgtgacgccc gccgagcaga tgcagtacgg caccgcgaag cacgtgcgga
     1501 tggcgggata cggcgaggac gccgccgggc gcgtcgtcga gctacgccag ctcgtcgacg
     1561 aatggcggcg cgggcgcgtg ccgatcgagc gtgcgcaggc ggagctcgac cgagcgaggc
     1621 gggaaccatg gttcgagcag gcctacctgc ccgagcggct gagcgccgcg aaggcgtggc
     1681 cggacatgga tttcgaaccg gccgaggtgt tcgcgaaggt gaccgtgccg gtgctcctct
     1741 tctacggcga ggacgacgag tggcagccga tcgacgcgag cgtggcggcg tggcggcgcg
     1801 ccgcggagcg cgcgggcaac gccgacctca ccatcgtgcg gctcgcgggg acggggcacg
     1861 cgccgacgat cggcgacctg gagacggtcg acgccgtcgc gcccgagtac gaacgagcgc
     1921 tgctcgagtg gcttcgacgc gtcgcgcggc taggctgagc tcgccgctcc ggccttcgct
     1981 cggcgcgcga cctcctcgcg cacggccggc gcgaccttcg tggcgaacag ctcgatcgac
     2041 cgcatgatct tctcgtgcgg gatcgagccg accgtcatct gcagcaggaa gcggtcgtga
     2101 tggaagatct cgtgctggaa caggatcttc tggatcacgt cctccgggtc cccgatgaaa
     2161 ttggcaccgc gaagagagcg ggagaagtcg aagtcggcgc gtgacatcgg cggccagccg
     2221 cgctcgcgtc cgatcctgtc catcatcagc ttgtacgggc cgtaggtcgt ctccgccgcc
     2281 tcgtcggcgg tgtccgcgac gtagccgtgc gagttgatcg acaggcgagg caggggaacg
     2341 tggcccgcgc tccgtgcggc ctcgcggtgg atctgggcga gcggtgcgaa gcgctcgggc
     2401 atcccgccga tgatcgcgag cgccatgggc aggcccaggg agccggcgcg caccgcggac
     2461 tcggcgttcc cgccgaccgc gatccagacc ggcagcgggt cctggaccgg gcgcgggtag
     2521 acgccctgcc cggcgagcgg cgcgcggtac gtgccggacc acgtgacgtg cgtgtcctcg
     2581 cgcacgcgca gcaggagccc gagcttctcg gcgaagagcg cgtcgtagtc cttgaggtcg
     2641 tgcccgaaca gcgggaacga ctcgatgaag gagccgcgtc cggccatgat ctcggcgcgg
     2701 ccgcccgaca ggaggtcgag cgtcgcgaag tcctggaaca cgcggaccgg atcgtccgag
     2761 ctgatgacgc tgacggcgct ggtgagccgg atccgctcgg tgcgcgccgc ggcggcggcg
     2821 aggacgaccg cgggggccga cacggcgaag tcgggacggt ggtgctcgcc gacgccgaag
     2881 acgtcgaggc cgagacggtc ggcgagctcg atctcctcga ggagatcgcg caggcgccgc
     2941 gccggactca ccgtcgtgcc gccggcgctc ggggtgagct cgccgaaggt atagaggccg
     3001 atctccatca gatcatccga acagccacgg cactagcatg attccgtgac cgggacgatg
     3061 cgcgcggcga cctccacgcc gctcaccgag gcgctctggc ggaagcgttt ccgcgcggcg
     3121 cggatcatgt tcccgtcctg ggcgcgcgac gatgccgacc acctcgtgta caccacgaac
     3181 agcagcggaa agttcgaggt gcatacctgg gaccgccgga acgacacgca ccggcagctg
     3241 accgaccgac ccgaaggcac cgggtatcgc gtcagctccc gcctcgagcc c
//
LOCUS       DASTZR010000473         4130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1096531, whole genome shotgun sequence.
ACCESSION   DASTZR010000473 DASTZR010000000
VERSION     DASTZR010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096531"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VFV20_10575"
     CDS             complement(<1..314)
                     /locus_tag="VFV20_10575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5288838.1"
                     /translation="MRILQVAPLVAPLDDRAVQLGGAQVVITELARRLASAGHEVTLA
                     AADGSFVEGVRLAPLGIDATRLERADLGMRGGARPDDAAERDAFARVRAWLDAHVADI
                     DVV"
     gene            complement(388..840)
                     /locus_tag="VFV20_10580"
     CDS             complement(388..840)
                     /locus_tag="VFV20_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288839.1"
                     /translation="MQTARMSHAGARTERVGALAPEITSPAYQAYLALRTGFVVLPIL
                     MGADKFLNALTNWEQYLAPVLPRAIGVTPELFMRGVGAIEIVAGLLVAVIPRYAGYVV
                     MAWLWLIIVNLVLRGEYWDVALRDFGLSVGAFALARLATSVHRFSASR"
     gene            951..1346
                     /locus_tag="VFV20_10585"
     CDS             951..1346
                     /locus_tag="VFV20_10585"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5288840.1"
                     /translation="MAKLTHVCVITADVSRLTEFYRHVLGMDPTRAHDDYVEFATPAG
                     ALSLYAQSKLEPYAPGATSPRRNRSVMIEFEVDDVDAEYERLRRAPIEWVKTPSTQPW
                     GNRSIYLRDPDGNLLNLYTRVGPTGERPA"
     gene            1390..2904
                     /locus_tag="VFV20_10590"
     CDS             1390..2904
                     /locus_tag="VFV20_10590"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008104196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEU5288841.1"
                     /translation="MRRKTALGGGEQRIQQQHAKGKLTARERVDLLLDEGSFTELDAF
                     VLARSSELVADDERAMGDGVVTGFGRIDGRLVYVFSQDFTVFGGSLAEAHAAKICKVM
                     DLAVRNGAPIIGLNDSGGARIQEGVVSLGGYADIFLRNVMASGVVPQLSAILGPCAGG
                     AVYSPAITDFIAMVRDTAYMFVTGPNVVKAVTHEDVTQDELGGADVHARRSGVAHFEA
                     DDEVSCLALLRTLIGYLPSNNLDPAPVVRGDDPVDRADSELDGIVPDVAAKPYDMRDV
                     IHRVVDGGEILEVHEGWARNIICAFARLDGTSVGVVAQQPMVLAGVLDIDASTKAARF
                     VRFCDAFNIPLVTFVDVPGFLPGTAQEHGGIIRSGAKLLYAYAEATVPKLTVITRKAY
                     GGAYDVMSSKHIRGDYNVAWPSAEIAVMGPEGAVNILYRDELAKAPDPDARRQELVAR
                     YVERYANPYVAAARGYIDDIIEPHETRPRLIAALRSLAGKRDTNPRRKHGNIPL"
     gene            2913..3254
                     /locus_tag="VFV20_10595"
     CDS             2913..3254
                     /locus_tag="VFV20_10595"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEU5288842.1"
                     /translation="MYGTIARLKLRKEKLREFLALGKEWDDRERKRATGYLGAEILWE
                     DREAGCACLIVHFSSRESYVANANSPEQDAFYKRLRECLESDPEWIDGSYGRWDSAYA
                     HPPDWSKSDRT"
     gene            3261..>4130
                     /locus_tag="VFV20_10600"
     CDS             3261..>4130
                     /locus_tag="VFV20_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEU5288843.1"
                     /translation="MTRPFRSVLVANRGEIALRIIRACRDLGMRAVAVYSDADRDALH
                     VRYADEAYPIGAAAPGESYLAIQKLVAVATRAGCDAVHPGYGFLSENADFAAAVEAAG
                     STFVGPPAPVQRLLGEKTRARRLARDAGVPVADGTIDPLEDLAEARSVAARVGYPVML
                     KAAGGGGGKGMRAVRSEGELAAAWRMASGEAASAFGTPALFLERLVEHARHVEVQLIA
                     DARGNVVWLGERDCSVQRRHQKLVEETPGPSVDAALRERLGDAAVKIARAAGYRNAGT
                     AEFLVAPDGSFSFL"
BASE COUNT          633 a         1452 c         1486 g          559 t
ORIGIN      
        1 acgacgtcga tgtccgccac gtgcgcgtcg agccacgcgc ggacacgagc gaaggcgtcc
       61 cgctcggcag cgtcgtccgg tcgggcgccg ccgcgcatgc ccaggtcggc gcgttccagg
      121 cgcgtcgcgt cgatcccgag cggcgcgagg cgcacgccct cgacgaacga gccatccgcc
      181 gccgcgagcg tgacctcgtg cccggcgctg gcgagccggc gcgcgagctc ggtgatgacg
      241 acctgcgcgc cgccgagctg gaccgcgcgg tcgtcaaggg gcgcgacgag gggagcgacc
      301 tggaggatgc gcacgcccgc actctgccac ggacggtgtg ccgggaggag cggcgcggca
      361 cgaggcacgt gccgcgccgc tcatatgtca gcgggacgcg ctgaagcggt ggacgctcgt
      421 ggcgagccgt gcgagcgcga acgctcccac ggagagaccg aagtcgcgga gcgcgacgtc
      481 ccagtactcg ccgcgaagga cgaggttcac gatgatcagc cagagccacg ccatcaccac
      541 gtagcccgcg tagcgcggga tcacggcgac gagcaggccg gcgacgatct cgatcgcgcc
      601 gacgccgcgc atgaagagct cgggcgtcac gccgatcgcg cgcggcagga ctggcgcgag
      661 gtactgctcc cagttcgtga gcgcgttcag gaacttgtcc gcgcccataa ggatcggcag
      721 gacgacgaat ccagtgcgga gcgcgaggta cgcctgatag gcggggctcg tgatctcggg
      781 cgcgagcgcg ccgacgcgct cggtgcgggc gcccgcgtgt gacatgcgtg ccgtctgcat
      841 cgcgtcacct cctcttccgc gagcacccgc acgagcgata ccagcgatcc ggcgccggcc
      901 gagcgtgggc gcgctcggtg cgtggcagcg cgcctgctgt ggcgtcggcc gtggcgaagc
      961 tcacacatgt ctgcgtcatc acggccgacg tgtcgcgcct gaccgagttc tacaggcacg
     1021 tcctcggcat ggatcccacg cgagcgcacg acgactacgt cgagttcgcc acgccggcgg
     1081 gagcgctctc gctgtacgcc cagtcgaagc tggagcccta tgcaccgggc gcgacgtcgc
     1141 cgcgccgcaa ccggagcgtc atgatcgaat tcgaggtcga cgacgtcgat gccgagtacg
     1201 agcgcctgcg ccgggcgccg atcgagtggg tgaagacgcc gagcacgcaa ccctggggaa
     1261 atcggtcgat ctacctccgc gatcccgacg ggaacctgct caacctctac acgcgcgtcg
     1321 gtccgacggg ggagcggccc gcatagacct atcctcagcg ccgtgagacc gaccgagcga
     1381 ctggaggaga tgcgccggaa gacggccctg ggcggcggcg agcagcgcat ccagcagcag
     1441 cacgccaagg gcaagctcac ggcacgcgag cgcgtcgacc tcctgctcga cgagggctcg
     1501 ttcaccgagc tcgacgcgtt cgtcctggcc cgctcctccg agctcgtcgc cgacgacgaa
     1561 cgcgcgatgg gcgacggcgt cgtgaccggc ttcggccgga tcgacggccg cctcgtgtac
     1621 gtcttctcgc aggacttcac cgtcttcggc gggtcgctcg cggaggcgca cgcggcgaag
     1681 atctgcaagg tcatggacct ggccgtgcgg aacggcgcgc cgatcatcgg gctgaacgac
     1741 tcgggtgggg cgcgcatcca ggagggcgtc gtctccctcg gcggctacgc cgacatcttc
     1801 ctgcgcaacg tgatggcatc gggcgtggtc ccgcagctgt cggcgatcct cggtccctgc
     1861 gcgggcggcg ccgtgtactc gcccgcgatc acggacttca tcgcgatggt gcgtgacacg
     1921 gcctacatgt tcgtgaccgg accgaacgtg gtgaaggccg tgacccacga ggacgtgacg
     1981 caggacgagc tcggcggcgc cgacgtgcac gcccggcgga gcggcgtggc gcacttcgaa
     2041 gcggacgacg aggtgtcgtg cctcgcgctg cttcgcactc tgatcggcta cctcccgtcg
     2101 aacaacctcg accccgctcc cgtcgtgcgc ggcgacgacc cggtcgatcg cgcggacagc
     2161 gagctcgacg gcatcgtgcc ggacgtggcg gcgaagccct acgacatgcg cgacgtcatc
     2221 caccgcgtgg tcgacggcgg cgagatcctc gaggtccacg agggctgggc ccggaacatc
     2281 atctgcgcgt tcgcgcgcct cgatgggacg agcgtgggcg tggtggcgca gcagcccatg
     2341 gtgctcgcgg gagtcctcga catcgacgcg tccaccaagg cggcgcgctt cgtccgcttc
     2401 tgcgacgcgt tcaacatccc gctcgtcacg ttcgtcgacg tgcccggctt ccttccgggg
     2461 accgcccagg agcacggcgg catcatccgg agcggcgcga agctgctcta cgcgtacgcc
     2521 gaggcgaccg tgccgaagct cacggtcatc acgcgcaagg cctacggcgg cgcgtacgac
     2581 gtcatgtcga gcaagcacat ccgcggcgat tacaacgtcg cctggccgag cgcggagatc
     2641 gcggtcatgg ggccggaggg cgcggtcaac atcctctatc gcgacgagct cgcgaaagcg
     2701 ccggatcccg acgcgcggcg gcaggagctt gtcgcgcggt acgtggagcg ctacgccaat
     2761 ccctacgtcg ccgccgcccg gggctacatc gacgacatca tcgagccgca cgagacgcgg
     2821 ccgcggctca tcgccgcgct gcggtcgctc gcaggcaagc gcgatacgaa cccgcgacgc
     2881 aaacacggca acatcccgtt gtaggaggag cgatgtacgg cacgatcgcg cggctgaagc
     2941 tgcgcaagga gaagctccgc gaattcctcg cgctcggcaa ggagtgggac gaccgcgagc
     3001 gcaagcgcgc gaccgggtac ctcggcgcgg agatcctgtg ggaggaccgc gaggccggct
     3061 gcgcgtgcct catcgtccac ttctcgagcc gcgagagcta cgtcgcgaac gcgaactcgc
     3121 ccgagcagga cgcgttctac aagcgcctgc gcgagtgtct tgaatccgat cccgagtgga
     3181 tcgacgggag ctacggtcgc tgggactccg cctacgcgca tccgcccgac tggagcaaga
     3241 gcgatcgcac gtaggctcac ctgacgcggc cgttccgctc ggtcctcgtc gcgaaccgcg
     3301 gcgagatcgc gctgcgcatc atccgcgcgt gccgcgacct cggcatgcgc gcggtcgccg
     3361 tctacagcga cgctgaccgc gacgcgctgc acgtccgcta cgccgacgag gcctacccga
     3421 tcggcgcggc cgcgccgggc gagagctatc tcgccatcca gaagctcgtg gcggtcgcga
     3481 cgcgcgcggg ctgcgacgcc gtgcatcccg gctacggctt cctgtcggag aacgcggact
     3541 tcgccgcggc ggtcgaagcg gccggcagca cgttcgtggg tccgcccgcc ccggtgcagc
     3601 gtctcctcgg cgagaagacg cgggcgcgac gtctcgctcg cgacgcgggc gtgcccgtcg
     3661 ccgacggcac gatcgacccg ctcgaggatc tcgccgaggc gcgcagcgtc gcggcgcgcg
     3721 tcggctatcc cgtgatgctg aaggccgcgg gcggcggcgg cggcaaggga atgcgcgccg
     3781 tgcggtcgga gggcgagctc gcggcggcct ggcgcatggc gagcggcgag gccgccagcg
     3841 ccttcggcac gccggccctc ttcctcgagc ggctcgtcga acacgcgcgc cacgtcgagg
     3901 tgcagctcat cgccgatgcg cgtgggaacg tcgtgtggct gggcgagcgc gactgctcgg
     3961 tgcagcggcg tcatcagaag ctcgtcgagg agacccccgg accgtcggtc gatgcggcgc
     4021 tgcgcgagcg gctcggtgac gccgcggtca agatcgcgcg cgcggccggc taccggaacg
     4081 ccgggacggc ggagttcctc gtcgcgccgg atggatcctt ctcgttcctc
//
LOCUS       DASTZR010000474         2700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_730267, whole genome shotgun sequence.
ACCESSION   DASTZR010000474 DASTZR010000000
VERSION     DASTZR010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730267"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /gene="xseA"
                     /locus_tag="VFV20_10605"
     CDS             complement(<1..544)
                     /gene="xseA"
                     /locus_tag="VFV20_10605"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU5288844.1"
                     /translation="MIRLAERIQSGDDFKDLWVEGEVVDTTISAAGHVYFTLRDQVGQ
                     LACVLFASQASQLALTPRNGTKLIAHGYVEYYHKASRVQLRVDDVRPAGMGDAYLRLE
                     ALKKRLTAEGIFAMERKRPLPTAPRRIGVVTSPIGAVWHDIQTVAARRDPRVELVLSP
                     ALVQGQASVDSLIGALDGLRQ"
     gene            complement(570..1130)
                     /gene="efp"
                     /locus_tag="VFV20_10610"
     CDS             complement(570..1130)
                     /gene="efp"
                     /locus_tag="VFV20_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU5288845.1"
                     /translation="MISTGEIKRGITVEIDGQLYQIVEFQHIKMGRGSAQVRMKLRNV
                     RKGDLIEKTVQAGERFQRARLDHRTVQFMYSDGTLYHFMDTKTYDQVALDATQLGDAV
                     NYLSDGMEVTLNEYEGEPIGVDMPASVVMTVASTDFGLKGDTATGATKPATLESGLKV
                     NVPLFVNAGDKIKVDTRTGEYLERSS"
     gene            complement(1114..1563)
                     /locus_tag="VFV20_10615"
     CDS             complement(1114..1563)
                     /locus_tag="VFV20_10615"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006838665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEU5288846.1"
                     /translation="MTRYLLVNGPNLNTLGTREPEIYGTTTLLEVEERCRAIARAANA
                     ELVTMQSNHEGALIDFLQKHADSDGAIINPGGLAHTSVVLRDAVAACRFPVVEVHISD
                     VTKRESFRHHSYLKDVTAYQVIGMGVVGYEVALRWLLTRSNSGDQHG"
     gene            complement(1560..>2700)
                     /gene="aroB"
                     /locus_tag="VFV20_10620"
     CDS             complement(1560..>2700)
                     /gene="aroB"
                     /locus_tag="VFV20_10620"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEU5288847.1"
                     /translation="DVDGATPEAIADAVVAWLARARGVRVDVGTGRPYPIVIRGGLVD
                     HLGEHVAALGWGGRIAIVTEPNAAALAERARRSLDRAGAPVAVVRAPSGEAAKTVRAV
                     SALWGALADAGVGRDGGVIAVGGGALGDAAGFAAATYLRGMPVVQVPTTLLAMVDASI
                     GGKTAIDIAAGKNLVGAFHQPQAVLIDTDALATLPRRQRSSGLAEVVKTAFLVDRASV
                     GHVERAIPGILAGDAGVALTAVTLAAEVKASVVTADPDERGLRALLNFGHTMGHAYEA
                     ASGYRVTHGEAVAVGLVYACALAEVLELAPAAIRSDVERLLLKAGLRIRAQIPRAAWA
                     LLARDKKVRAGKVRWILPRRIGRFSEVTDVGERALRAAARVVEAV"
BASE COUNT          387 a          975 c          925 g          413 t
ORIGIN      
        1 gctgacggag gccgtcgagc gcgccgatga gcgagtcgac cgaggcctgg ccctggacca
       61 gcgcgggtga cagcacgagc tcgacccgcg gatcgcggcg cgcggcgacg gtctggatgt
      121 cgtgccatac ggcgccgatc gggctcgtca cgacgccgat gcgccgcggt gccgtcggca
      181 gcgggcgctt gcgctccatc gcgaagatgc cttccgcggt gaggcgcttc ttcagtgcct
      241 cgagacggag gtacgcgtcg cccatgcccg ccggacggac gtcatcgacg cggagctgga
      301 cgcggctcgc cttgtggtag tactcgacgt acccgtgcgc gatgagcttc gtgccgttgc
      361 gcggcgtgag ggccagctgc gaagcctgcg acgcgaagag cacgcacgcc agttggccca
      421 cctgatcgcg cagcgtgaag tacacgtggc ccgcggccga gatggtcgtg tccacgacct
      481 cgccctcgac ccacaggtcc ttgaagtcgt cgcccgattg gatgcgctcg gccagccgga
      541 tcaccagctg gccgacgcga aggacgtcct taggaggagc gctcgagata ctcacccgtc
      601 cgtgtatcga ccttgatctt gtcgccggcg ttcacgaaga gcggcacgtt caccttcagg
      661 cccgactcga gcgtcgccgg cttcgtggcc ccggtcgcgg tgtcgccctt caggccgaag
      721 tccgtcgagg cgacggtcat gacgaccgac gcgggcatgt cgacgccgat cggctcgccc
      781 tcgtattcgt tcagcgtcac ctccatgccg tcgctgaggt agttcaccgc atcgccgagc
      841 tgggtcgcgt cgagcgcgac ctggtcgtac gtcttcgtgt ccatgaagtg gtagagcgtc
      901 ccgtccgagt acatgaactg caccgtgcgg tggtcgagcc gcgcgcgctg gaagcgctcg
      961 ccggcctgca ccgtcttctc gatgaggtcg cccttgcgca cgttgcggag cttcatgcgc
     1021 acctgcgccg agccgcggcc catcttgatg tgctggaact cgacgatctg atagagctgg
     1081 ccgtcgatct cgacggtgat gccgcgcttg atctcacccg tgctgatcac cagagttgct
     1141 cctcgttagt agccagcgca gagcgacctc gtagccgacg acgcccatgc cgatgacttg
     1201 atacgcggtg acgtccttga gatagctatg gtgacggaaa ctctcccgct tcgtcacgtc
     1261 gctgatgtgg acctcgacga ccgggaaacg gcacgccgcg accgcgtcac gcaggacgac
     1321 cgacgtgtgg gcgagcccgc caggattgat gatggcacca tctgaatcgg cgtgcttttg
     1381 caggaagtcg atgagcgcgc cctcgtggtt cgactgcatc gtcacgagct cggcgttggc
     1441 ggcccgcgcg atggcgcggc agcgctcctc gacctcgagg agcgtcgtcg tgccgtagat
     1501 ctccggctcg cgcgtcccca gggtgttcag gttcggtccg ttcaccagca gatagcgcgt
     1561 cataccgcct ccaccacgcg ggcggccgct cggagcgccc gctcaccgac gtcggtcacc
     1621 tcgctgaagc ggccgatgcg gcgcggcagg atccagcgga ccttccccgc ccgcaccttc
     1681 ttatcacggg cgaggagtgc ccacgccgcg cgcgggatct gcgcccggat acgcagtccg
     1741 gccttcagca gcagccgttc gacgtccgac ctgatcgccg ccggggccag ctccaggacc
     1801 tcggcgagcg cgcacgcgta caccagcccg acggccacgg cctcgccgtg ggtcacgcgg
     1861 tacccgctcg cggcctcgta cgcgtggccc atggtgtgcc cgaagttgag gagcgcccgc
     1921 agcccgcgct cgtcgggatc ggcggtgacg acgctcgcct tgacctcggc cgcaagcgtc
     1981 accgcggtga gggccacgcc cgcgtccccc gcgaggatgc ccgggatggc acgttcgacg
     2041 tggcccaccg aggcccgatc gacgaggaag gccgtcttca ccacctccgc cagacccgag
     2101 gagcgctgac ggcgcgggag cgtcgcgagc gcgtcggtgt cgatgaggac ggcctgcggc
     2161 tggtggaacg ccccgaccag gttcttgccg gccgcgatgt cgatcgccgt cttgccgccg
     2221 atgctcgcgt ccaccatcgc gagcagcgtc gtcggcacct gcacgacggg catgccacga
     2281 aggtaggtcg ccgccgcgaa gcccgccgcg tcgccgagcg cgccaccgcc gaccgcgatc
     2341 acgcccccgt cccggcccac gcccgcgtcc gcgagcgcgc cccagagggc cgagaccgcg
     2401 cgcaccgtct tggccgcctc gccggatggc gcgcgcacga cggcgaccgg cgcgccggcg
     2461 cggtcgagcg agcgccgcgc gcgttcggcg agcgctgccg cgttcggctc ggtcacgatc
     2521 gcgatgcggc cgccccagcc aagcgccgcg acgtgctcgc ccagatggtc gacgaggccg
     2581 ccgcggatga cgatcgggta tggacgcccg gtcccgacat cgacgcgcac gccgcgcgct
     2641 cgcgcgagcc aggccacgac cgcgtcggcg atcgcttcgg gcgtcgcgcc gtcgacgtcg
//
LOCUS       DASTZR010000475         2523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_46051, whole genome shotgun sequence.
ACCESSION   DASTZR010000475 DASTZR010000000
VERSION     DASTZR010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46051"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="VFV20_10625"
     CDS             <1..920
                     /locus_tag="VFV20_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288848.1"
                     /translation="DASGNVVVPDGVRTRLPRDVAPGTTITVDLSVVVPPSVGTHVLR
                     VDLFREGIGWFSLRGVRPADVEVTIEAPHAAAYGPLTASVWLTLPSAAPLVVTLKNTG
                     TTAWEAGGPNPVRLSYHWMRNGQLALWDGARAALAKDVLPGETVIVELPVIPPEGLSA
                     HTLRLDLVKEGVRWFSDEGVATRDVSYTVASGYGAAYGVASPSAAGAPLTVTLTNTGV
                     RPWVAAGPHPVRLAYHLLRDGAVVVWDGPRIPLGRDVQPGERVTLQLPVAMPATNGSY
                     AMRLDLVEEGVRWFSDAGIAGRDVPVPAD"
     gene            complement(921..>2523)
                     /locus_tag="VFV20_10630"
     CDS             complement(921..>2523)
                     /locus_tag="VFV20_10630"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU5288849.1"
                     /translation="AGYALLQAAGADPVRWADLDSKARQFATLGNPDFYGQFLSAAAI
                     AGAAIALFSDTPRLRALAGGLAALSTVLLVLTATRGAVIGLVAGAIALGVLWLRRAGA
                     TRTALARLALAGAAFVAALGLAVLTTPLGDRLVALTEVANIQDRVLIYSSATRIFLDH
                     PVLGVGFENFAVAYPGYEEAEGIKNNRTQTSAHNWLLHVAATTGLVGLAATAAVLGAF
                     AFHVWRRARDPDAPALLAAAGALVAFYASGLVLPGAQSIQWIPWACLGVALASDLRTA
                     PVAMRLPPVRVPLLAVIAGLVVLMVLALAQTTVVTANRLAKTAETSLRAETAQRAVDA
                     ARGATDADPGRAVYWNDLGRALELVDDQAGARRAYREAVERSPYTPAFWWNLGRMHLF
                     FARRGEEGAKAASYDAFRRGLEASPRNPDTYDQLARAQLALGDLADALASEERAIALY
                     PGDPRYYTTAADAARLQGASTRSLDLLRAGVAATGSNELRLTLARRLIDARSENEARA
                     VLNEVLRIEPGNAAAAELLRQLGGP"
BASE COUNT          336 a          899 c          961 g          327 t
ORIGIN      
        1 tcgacgcgag cggcaacgtc gtagtgccgg acggcgtacg gacgcgcttg ccccgcgacg
       61 tcgcgccggg gacgaccatc acggtggacc tgagcgtcgt cgtgcccccg tcggtcggga
      121 cccacgtcct gcgcgtcgac ctcttccgcg agggcatcgg atggttctcg ctgcgcggcg
      181 tgcgcccggc cgacgtcgag gtcacgatcg aagcgcccca tgccgcggcg tacgggccgc
      241 tcaccgcgag tgtctggttg accctgccga gcgcggctcc gctcgtcgtc accctgaaga
      301 acacgggcac gaccgcatgg gaggcgggtg ggccgaatcc ggtgcggctc tcgtaccact
      361 ggatgcgcaa cggccagctc gcgctgtggg acggggcgcg cgccgcgctc gcgaaggacg
      421 tgctgcctgg tgagaccgtg atcgtcgagc tcccggtgat cccgccggag ggcctctccg
      481 cgcacaccct gcgtctcgat ctcgtgaaag agggcgttcg ctggttcagc gacgagggcg
      541 tcgcgacgcg cgacgtctcg tataccgtcg cgtccgggta cggcgctgcg tacggcgtcg
      601 cgagcccgtc ggccgccggc gcgccgctga ccgtcaccct gacgaacacg ggcgtgcgac
      661 cgtgggtcgc ggccggtccg cacccggtgc ggctcgcgta ccacctgctg cgcgacggcg
      721 ccgtcgtggt gtgggacggg cctcgcatcc cgctggggcg cgacgtgcag cccggcgagc
      781 gcgtgacgct ccagctgccc gtcgcaatgc cggcgacgaa cgggtcgtac gcgatgcgcc
      841 tcgatctcgt cgaggagggc gtgcgctggt tcagcgatgc ggggatcgcg ggacgcgacg
      901 tgccggttcc cgccgactga tcacggcccg ccgagctgcc gcagcagctc cgcggccgcc
      961 gcgttccccg gctcgatccg cagcacctcg ttcagcaccg ctcgcgcttc gttctcgctc
     1021 cgcgcgtcga tgaggcggcg ggccagcgtg agccgtagct cgttcgagcc ggtcgccgcg
     1081 acaccggcgc gcagcaggtc gaggctccgc gtgctggctc cctggagccg cgcggcgtcg
     1141 gcggcggtcg tgtagtaccg cggatcgccg ggatacagcg cgatcgcgcg ctcctcgctc
     1201 gcgagcgcgt cggcgaggtc gccgagcgcg agctgcgctc gcgcgagctg gtcgtacgtg
     1261 tcggggttgc ggggcgacgc ctcgagcccg cgccggaagg cgtcgtagct cgcggccttc
     1321 gcgccctcct ccccgcggcg cgcgaagaag aggtgcatgc ggccgaggtt ccaccagaac
     1381 gccggcgtgt acggagagcg ctcgacggcc tcgcggtacg cgcgccgcgc tccggcctga
     1441 tcgtccacga gctcgagcgc gcggccgaga tcgttccagt acacggcgcg cccggggtcc
     1501 gcgtcggtcg caccgcgcgc ggcgtcgacc gcacgctgtg cggtctccgc acggagcgag
     1561 gtctccgccg tcttcgcgag ccggttcgcc gtgaccacgg tcgtctgtgc gagcgcgagc
     1621 accatgagga ccacgaggcc cgcgatgacc gcgaggagcg ggacgcggac cggcggaagc
     1681 cgcatcgcga ccggtgccgt cctgaggtcg ctcgcgagcg cgaccccgag gcacgcccac
     1741 gggatccact ggatgctctg cgcgccgggc agcacgaggc cgctggcgta gaacgcgacg
     1801 agcgcgccgg cggccgcgag gagcgccggg gcatccggat cgcgcgcacg acgccagacg
     1861 tggaacgcga aggcgcccag gaccgcggcc gtcgccgcga ggccgaccag gcccgtcgtc
     1921 gccgcgacgt ggaggagcca gttgtgggcc gacgtctgcg tgcggttgtt cttgatgccc
     1981 tcagcttcct cgtacccagg gtaggcgacg gcgaagttct cgaagccgac cccgaggacc
     2041 gggtgatcga ggaagatccg cgtcgcggat gaatagataa ggacacggtc ctggatgttg
     2101 gccacctcgg tgagggcgac gagccggtcg ccgagcggcg tcgtgagcac ggcgaggccg
     2161 agcgccgcca cgaaagccgc gcccgcgagg gccagacgtg cgagcgccgt ccgcgtggcg
     2221 ccggcacgcc tgagccagag cacgccgagg gcgatggccc ccgcgaccag gccgatgacg
     2281 gcgccgcggg tcgcggtgag gacgaggagg accgtgctga gcgccgcaag accacccgcg
     2341 agcgcgcgca ggcgcggcgt atctgagaac agcgcgatcg cggctcccgc gatcgccgcg
     2401 gcggagagga actggccgta gaagtcgggg ttgccgaggg tcgcgaactg acgcgccttc
     2461 gagtcgaggt ccgcccaccg cacggggtcc gcgcccgcgg cctggaggag cgcgtagcct
     2521 gcc
//
LOCUS       DASTZR010000476         2279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_333055, whole genome shotgun sequence.
ACCESSION   DASTZR010000476 DASTZR010000000
VERSION     DASTZR010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333055"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VFV20_10635"
     CDS             <1..288
                     /locus_tag="VFV20_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288850.1"
                     /translation="IGDLLIAELAKRRLDVVRGRLTLASTDFGNVSQVAPADYIGFPV
                     STDPVPGHSHHMREASASDYAHDAAMTTAEVLATVAVRIATDRVTRDSLRS"
     gene            complement(285..>2279)
                     /locus_tag="VFV20_10640"
     CDS             complement(285..>2279)
                     /locus_tag="VFV20_10640"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEU5288851.1"
                     /translation="IAEAAQELGAATFAVRSSSPNEDLAVSTSAGRYLTVLGVRPDEL
                     DRTVERVYGSHDPGSAMAVLVQPMLAASAAGVAFTADPLTGDADVVTVTAVRGLGDRL
                     VGGEATAEEWRVARGRLARVRDANVPALSAVDAEAVVALARAIAARRGSAQDLEWAIV
                     GGAVHVLQARPMTGLPEPVRWDAPPGAFARSFRLGEWIGDPVTPLFESWLLSVMERTM
                     HGDYERLVGQPAPRPLHVVVNGWYYYSLNFLPASPMAIARMLPGIIARLPRHARRIAP
                     IFPPLARFGIDVYVREWRDELLPAYREAVAAAASAVDATPIPRLPRLIDELAERAGHY
                     FTSVTFVAGYGWKTEIPLAQFYRAHLQARVGGDYRQLIRGLSAQRLRPHAVHSLDWWF
                     PALGETAEASDVDGGVNAGVVAERELAERRCRETLDPPLRARFERLLAEAQRGAVLRE
                     EQVAELTLPWPTLRAALRRIGTELVARGAIGAIDDVYFLRRDELDAALSDPNLRFTDL
                     AADRRSIWERQRRLIAPLVIGTMPRMLATILGAADRAMRDPVDAPEHAIRGIPASAGR
                     VTGPARVIWDADDFARVRQGDVLVCRVMSPASSALFGIAAAIVTDVGSPAAHASVIAR
                     EFGIPAVVGTQDATARIIDGTLVTVDGGAGLVWVEGAPRP"
BASE COUNT          328 a          820 c          846 g          285 t
ORIGIN      
        1 atcggcgacc tgctcatcgc ggagctcgcg aagcggcggc tcgacgtggt gcgcggccgg
       61 ctcacgctcg cgtccacgga cttcggcaac gtctcgcagg tcgcgcccgc ggactacatc
      121 ggcttcccgg tgagcaccga cccggtcccg ggccattcgc accacatgcg cgaggcgtcg
      181 gcgagcgact acgcgcacga cgcggcgatg accacggccg aggtgctcgc gaccgtggcg
      241 gtgcgcatcg ctacggatcg cgtgacgcgg gacagcctcc gctcctaggg ccggggcgcg
      301 ccctcgaccc agacgaggcc cgcgccgccg tcgaccgtga cgagcgtgcc gtcgatgatc
      361 cgcgccgtcg cgtcctgcgt cccgacgacg gcggggatgc cgaattcgcg tgcgatgacc
      421 gaggcgtgtg cggcgggact gcccacgtcc gtgacgatcg ccgccgcgat gccgaacagc
      481 gcgcttgacg cgggggacat cacgcggcag acaaggacgt cgccctgccg cacgcgcgcg
      541 aagtcgtccg cgtcccagat cacccgtgcc gggccggtga cgcgtccggc gctcgcgggg
      601 atgccgcgga tcgcgtgctc cggcgcatcg accgggtcgc gcatcgcgcg gtccgcggcg
      661 ccgaggatgg tggcgagcat gcgcggcatg gtgccgatga ccagtggcgc gatcaggcgg
      721 cgttgccgtt cccagatgga acgacgatcc gcggcgaggt ccgtgaaccg cagattcggg
      781 tcggacagcg ctgcgtcgag ctcatcgcgg cgcaggaagt agacgtcgtc gatcgcgccg
      841 atcgctccgc gcgcgaccaa ctcggtgccg atgcgccgca gggcggcacg caaggtcggc
      901 catggcagcg tgagctcggc gacctgttcc tcccgcagca ccgcaccgcg ctgcgcttcc
      961 gcgaggagcc gctcgaaccg ggcacggagg ggcgggtcga gcgtctcgcg gcagcgtcgc
     1021 tcggcgagct cgcgctccgc gacgacgccg gcgttcacgc cgccgtccac gtcgctcgcc
     1081 tcggccgtct cgcccagggc ggggaaccac cagtcgagcg aatgcaccgc gtgcggccgg
     1141 aggcgctggg cggagagccc ccggatcagc tgccggtagt cgccgccgac gcgcgcctgc
     1201 aggtgcgcgc gatagaactg cgccaggggg atctcggtct tccagccgta tcccgcgacg
     1261 aacgtgaccg acgtgaagta atgccccgca cgctccgcaa gctcgtcgat gagacgcggc
     1321 agccgcggga tcggcgtcgc gtcgacggcg ctcgccgcgg ccgccaccgc ctcgcggtat
     1381 gccggaagca gctcgtcccg ccattcgcgc acgtacacgt cgatgccgaa gcgcgcgagg
     1441 ggcgggaaga tcggggcgat gcggcgggca tgtcgcggca ggcgcgcgat gatgcctggg
     1501 agcatccgcg cgatcgccat gggggatgcc ggcaggaagt tgagcgagta gtagtaccag
     1561 ccgttcacga cgacatgcag cggacgcgga gcgggctggc cgacgaggcg ctcgtaatcg
     1621 ccgtgcatcg tccgctccat gacggacagg agccagctct cgaagagcgg cgtgaccgga
     1681 tcgccgatcc actcaccgag gcggaagctg cgggcgaagg cgccgggtgg agcgtcccac
     1741 cgcacgggct cgggcagtcc ggtcatcgga cgcgcttgga ggacgtgcac cgcgccgccc
     1801 acgatcgccc actcgaggtc ctgcgccgag ccgcgtcgcg ccgcgatggc acgcgcgagc
     1861 gcgacgacgg cctccgcgtc gacggcgctc agggcgggaa cgttcgcgtc gcgcacgcgg
     1921 gccaacctgc cccgcgcgac gcgccattcc tccgcggtcg cctcgccgcc gacgagccga
     1981 tcgccgagtc cgcggacggc cgtcacggtg acgacatcgg cgtcgcccgt gagcggatcc
     2041 gccgtgaacg cgacgcccgc cgcgctcgcc gcgagcatcg gctggaccag gacggccatc
     2101 gcgcttccgg ggtcgtggct gccgtacacg cgctcgacgg tgcgatcgag ctcatcgggc
     2161 cggactccga ggaccgtcag gtagcggcct gcggatgtcg agaccgcgag atcctcgttg
     2221 ggcgacgacg agcggacggc gaacgtggcc gcgccgagct cctgcgcggc ttccgcgat
//
LOCUS       DASTZR010000477         3844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_677950, whole genome shotgun sequence.
ACCESSION   DASTZR010000477 DASTZR010000000
VERSION     DASTZR010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677950"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /gene="nusA"
                     /locus_tag="VFV20_10645"
     CDS             <1..648
                     /gene="nusA"
                     /locus_tag="VFV20_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010630918.1"
                     /note="modifies transcription through interactions with
                     RNA polymerase affecting elongation, readthrough,
                     termination, and antitermination; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEU5288852.1"
                     /translation="VSRSHKALVKRLFELEVPEIYHGQVEIVGIAREAGSRTKIAVRA
                     RQPGIDARGACIGQRGLRVQAIVNELGGEKIDIIEWSEKPEQYVANALEPAHVVRVDI
                     VVDDKTAYVVVPDRQLSLAIGKEGQNARLAAKLTGWRIDIRSESEVRAEAEPEPEPEV
                     EAAPAEAAPVAEAEDAEIRHDLVAEVEAESALADEEDLDEEDRALLGGKKKRERR"
     gene            596..955
                     /locus_tag="VFV20_10650"
     CDS             596..955
                     /locus_tag="VFV20_10650"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEU5288853.1"
                     /translation="MRRTARSSEERRSASVASRGGARALPQRTCVACRTVRAKRELVR
                     IVRAPTGELSVDLRGKAAGRGAYLDPDPGCLERGLNEGVLARALEITIDDATAERLRA
                     ELDDAARTRSQEQEEKR"
     gene            955..2859
                     /gene="infB"
                     /locus_tag="VFV20_10655"
     CDS             955..2859
                     /gene="infB"
                     /locus_tag="VFV20_10655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU5288854.1"
                     /translation="MPKARPRRLPPQRGPRPRGRRPQRPQQQAPAPVVVAAPVAVATG
                     RPPVVLPKTMLVSELARLLEVPVVDVIRALVGMGEMKTINQSIDFETATLVSNELGIE
                     TTAEEEAPAPEEPAEEVPIAKLDLWTEEDATKLRDRPPVVTVLGHVDHGKTSLLDAIR
                     STNVTSREAGGITQHIGAYQIEHNDRKVTFLDTPGHEAFTAMRARGAQITDVAVLVVA
                     ADDGVQPQTIEAIAHVKAAGVPMVVALNKIDKADANPDQVKAQLAERGVTIEEYGGDT
                     PLVPVSARTKQGIADLLEVVMLVADVAELKANPDRPAGGRVVEAHLDAKRGPVATVLV
                     QTGTLERGDLVVAGVTYGRVRAMTDDKGKTVGRAEPSRPVEILGLPNVPEAGDIFRAV
                     ADEKTAKALVAQNERERSGGAGAERPATLDEMFAQVKEGRAKELKVVLKADVQGSLEA
                     IKGALAKIPQEEVGLQVIHDGVGDITESDLTLAAASGAVVIGFNTKMEAPARRAADAT
                     NVDVRQYKVIYELLEEVQKALTGMLEPEMVESILGHAEVRQIFTAGKTTIAGCMVLDG
                     TMRRGAQARLIRAGAPAWEGSIGSLRRMKDDVREVNAGLECGIVLEGTNDEQAGDVIE
                     AFVVQPKPRG"
     gene            2856..3215
                     /gene="rbfA"
                     /locus_tag="VFV20_10660"
     CDS             2856..3215
                     /gene="rbfA"
                     /locus_tag="VFV20_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011230765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU5288855.1"
                     /translation="MTFKRAERVNALLQRELGMIISEELRDPRIAFSTVTAVETTDDL
                     RTAKVHVSVLGDDEQIAETMRALEEAKPYLRHELGSRVALRFVPDLTFLSDRTAERAA
                     RISTLLREAKGGGPKPE"
     gene            3212..>3844
                     /locus_tag="VFV20_10665"
     CDS             3212..>3844
                     /locus_tag="VFV20_10665"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEU5288856.1"
                     /translation="MNDVVKELRQAQRVAIVAHRDPDPDTIGSAIALGIALESLGKQV
                     SLHCADGVPEAHQFLPRAETIVTAAPPADVDLIVTVDFGSLERAKFALPKGPRLVNID
                     HHASNERFGDVNLVDVSSAACAEIVSRIIDALDVAWTADMATAALVGIMTDTGSFQFP
                     STDARVLERAARLRDAGADLQAITYNIFRNKRFEALRLYGHAFARMRREHH"
BASE COUNT          658 a         1322 c         1389 g          475 t
ORIGIN      
        1 gtcagccgct cgcacaaggc gctggtgaag cgcctgttcg agctcgaggt gcccgagatc
       61 taccacgggc aggtcgagat cgtgggcatc gcccgcgagg cgggttcgcg caccaagatc
      121 gccgtccgcg cgcgccagcc cggcatcgac gcgcgcggcg cgtgcatcgg ccagcggggc
      181 ctccgcgtcc aggcgatcgt gaacgagctc ggcggcgaga agatcgacat catcgagtgg
      241 tcggagaagc ccgagcagta cgtcgccaat gcgctcgagc cggcgcacgt cgtgcgcgtc
      301 gacatcgtcg tcgacgacaa gaccgcgtac gtcgtcgtgc cggaccgcca gctctcgctt
      361 gcgatcggca aggagggcca gaacgcccgc ctcgcggcca agctcacggg ctggcgcatc
      421 gacatccgga gcgagtccga ggtgcgcgcc gaggccgagc cggagcccga gcccgaggtc
      481 gaggccgcac cggccgaggc cgcgccggtg gccgaggcgg aggacgcgga gatccgtcac
      541 gacctcgtcg cggaggtcga ggcggagtcg gcgctcgcgg acgaggagga ccttgatgag
      601 gaggaccgcg cgctcctcgg aggaaagaag aagcgcgagc gtcgctagca ggggcggcgc
      661 tcgggccctt ccacagcgga cctgcgtggc gtgccgcacc gtccgggcga agcgcgagct
      721 cgttcggatc gtacgggcgc cgacgggtga gctttcggtc gaccttcgcg ggaaggcggc
      781 gggccgaggg gcgtaccttg atcctgaccc ggggtgcctc gagcgggggc tgaacgaggg
      841 agtcctggcc cgcgcgctcg agatcaccat tgacgacgcg accgcggaac gcctccgagc
      901 ggagctggac gacgcggcga ggacacggtc gcaagagcaa gaggagaagc gctgatgcca
      961 aaagcaagac cacggcgact gccgccgcag cgcggcccgc gtccacgggg acggcggccg
     1021 cagcgtcccc agcagcaggc gcccgccccc gtcgtcgtcg cggcgccggt cgcggtcgcg
     1081 accgggcgtc cgcccgtcgt gctgccgaag acgatgctgg tctccgagct cgcacggctc
     1141 ctcgaggtgc cggtcgtcga cgtcatacgc gcgctcgtcg gcatgggcga gatgaagacg
     1201 atcaaccaga gcatcgactt cgagaccgcc acgctggtgt cgaacgagct cggcatcgag
     1261 accaccgccg aggaggaggc gcccgcgccc gaggagccgg ccgaggaggt ccccatcgcg
     1321 aagctggacc tctggaccga ggaggacgcc accaagctga gggaccgtcc gcccgtcgtg
     1381 accgtcctcg gacacgtcga ccacggcaag accagcctcc tcgacgcgat ccgctcgacg
     1441 aacgtgacct cacgcgaggc cggcggcatc acacagcaca tcggcgcgta ccagatcgaa
     1501 cacaacgacc ggaaggtgac gttcctcgat acgcccggtc acgaggcgtt cacggccatg
     1561 cgcgcccgcg gcgcgcagat caccgacgtc gccgtgctcg tcgtcgccgc cgacgacggc
     1621 gtgcagccgc agacgatcga ggccatcgcg cacgtgaagg ccgcgggtgt gccgatggtc
     1681 gtggcgctga acaagatcga caaggccgac gcgaaccccg atcaggtgaa ggcgcagctc
     1741 gcggagcgcg gcgtcaccat cgaggagtac ggcggcgaca cgccgctcgt cccggtctcc
     1801 gcccgcacca agcagggcat cgcggatctc ctcgaggtcg tgatgctcgt cgcggacgtc
     1861 gcggagctca aggcgaaccc cgaccggccg gcgggcggtc gcgtggtcga ggcgcacctc
     1921 gacgccaagc gcgggccggt cgccaccgtg ctcgtgcaga cgggcacgct cgagcgcggc
     1981 gacctcgtcg tcgcgggcgt cacctacgga cgcgttcgcg cgatgaccga cgacaagggg
     2041 aagaccgtgg ggcgcgcgga gccgtcgcgg cccgtcgaga tcctcggcct cccgaacgtc
     2101 cccgaggcag gcgacatctt ccgcgcggtc gcggacgaga agaccgcgaa ggcgctcgtc
     2161 gcccagaacg agcgcgaacg gagcggcggc gcgggcgcgg agcgtcccgc gacgctcgac
     2221 gagatgttcg cgcaggtcaa ggagggcagg gccaaggagc tgaaggtcgt cctcaaggcg
     2281 gacgtccagg gctcgctcga ggcgatcaag ggcgcgctcg cgaagatccc gcaggaagag
     2341 gtcggcctgc aggtgatcca cgacggcgtc ggcgacatca ccgagtcgga tctcacgctc
     2401 gccgccgcct cgggtgcggt ggtcatcggc ttcaacacga agatggaggc gccggcccgc
     2461 cgtgcggccg acgcgaccaa cgtcgacgtg cgccagtaca aggtcatcta cgagctgctc
     2521 gaggaggtcc agaaggcgct caccggcatg ctcgagccgg agatggtcga gagcatcctc
     2581 ggccacgcgg aggtgcggca gatcttcacc gccggcaaga cgaccatcgc cggatgcatg
     2641 gtgctcgacg gcacgatgcg gcgaggcgcg caggcgcggc tcatccgcgc cggtgccccg
     2701 gcgtgggagg gctccatcgg ctcgctccgt cgcatgaagg acgacgtgcg tgaggtcaac
     2761 gcgggcctgg agtgcgggat cgtgctcgag ggcacgaacg acgagcaggc cggggacgtc
     2821 atcgaggcct tcgtcgtgca gccgaagccg cgaggatgac cttcaagcgg gccgagcgcg
     2881 tgaacgcgct cctgcagcgc gagctcggca tgatcatcag cgaggagctg cgcgatccgc
     2941 gcatcgcctt ctcgacggtc acggccgtcg agacgacaga cgacctgcgg acggcgaagg
     3001 tgcacgtcag cgtgctcggc gacgacgagc agatcgccga gacgatgcgc gcactggaag
     3061 aggcgaagcc gtacctccgt cacgagctcg gctcgcgcgt cgcgctccgt ttcgtgccgg
     3121 accttacgtt cctatccgac cgtacggcag agcgggccgc gcgcatcagc acgctgctgc
     3181 gggaggccaa gggcggcgga ccgaagccgg aatgaacgac gtcgtgaagg agctgcggca
     3241 ggcgcagcgc gtggccatcg tggctcaccg cgatccggac cccgacacga tcggctcggc
     3301 catcgccctg ggcatcgcgc tcgagtcact ggggaagcag gtgagcctcc actgcgccga
     3361 cggcgtcccg gaagcccacc aattcctgcc gcgggcggag accatcgtca ccgccgcgcc
     3421 gccggccgat gtcgatctca tcgtgacggt cgacttcggc tcgctggagc gagcgaaatt
     3481 cgcgctgcca aagggcccgc gtctggtgaa catcgaccac cacgcgtcca acgagcgctt
     3541 cggcgacgtg aacctggtcg acgtgtcgtc cgcggcctgc gccgagatcg tctcgcgcat
     3601 catcgacgcg ctcgacgtgg cgtggacggc ggacatggcg acggccgcgc tcgtgggcat
     3661 catgaccgac acgggctcgt tccagttccc gtccaccgac gcgcgcgtcc tcgaacgtgc
     3721 ggcacgtctc cgcgacgctg gagccgacct gcaggccatc acctacaaca tcttccgcaa
     3781 caagcggttc gaggcgctgc ggctctacgg ccacgcattc gctcgcatgc ggcgcgagca
     3841 ccat
//
LOCUS       DASTZR010000478         3214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_385849, whole genome shotgun sequence.
ACCESSION   DASTZR010000478 DASTZR010000000
VERSION     DASTZR010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385849"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..639)
                     /locus_tag="VFV20_10670"
     CDS             complement(139..639)
                     /locus_tag="VFV20_10670"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEU5288857.1"
                     /translation="MPSQTTAAITERKTRLDGSVAEFACEAIVVEPGRRAVLRYVTDR
                     EWAISGSDIRVPAGTVTVGHYWVDRPYNVYHWLVGGRTLAYYCNVATDTTIVPALVAY
                     RDLTVDVLIRTSGEPTVLDEDDLPDDLAPPYRLAIAKALESLIANPRMVIREVERETA
                     AALRGR"
     gene            692..2716
                     /locus_tag="VFV20_10675"
     CDS             692..2716
                     /locus_tag="VFV20_10675"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5288858.1"
                     /translation="MRELYQVVEVGPAWYQENIPCQEACPVRTNCRGYLNLAAAGEFE
                     KAWELALDPNPMASICGSVCAAPCETACRRKEVDKPLSIRYMKKFLSDWTHQNITVNG
                     RAYTQPAPPVFGPPRGKVAIVGAGCAGLGAASDLAKLGFGVTVLDALDQAGGTALAGV
                     PPFRLPREVIDRDINGIVNENVELRLSTYVGRDVTLRQLHDEYDAVLVTAGCFEPNYP
                     GIPGEDADGVMAGIVFLERAYRGRPLEVGKRVVVLGGGYTAMDCASTSWRLGADDVYI
                     VYRRSRDEMVVDEEELGETEREGMRFVYLASPVAILTNDAGHVRGVRFIRNKLGEPDK
                     SGRRSPEPIEGSEFDIECDTVLLALGQAPDTTFLAEFPEFRVKRWRDVKLDADTYQTV
                     VPKVFMAGDYRTGPTTIIEAVAEGRSAAQQIARFLDPLAVAVNIPEYHEIVTLRRSST
                     PDNVGQIAGEGELARIYHNMSVDYDRIPRQEMPKLAKKERRGLFLEVNQGYSEAQAVA
                     EGDRCLKCNFNIEIIGELCIICGGCVDVCPMDVIHMVDMSDIVDDGAIPGVGEAKKWD
                     RGVAFFLDETACIRCALCVIRCPTDAITMNRYGAVMPTIGKTLPKESIDDEIHLDLTV
                     ASRKSIRPLPMYDPALAAGYKPASNGHANGNGANGHNGKPAREKEREAVH"
     gene            2720..>3214
                     /locus_tag="VFV20_10680"
     CDS             2720..>3214
                     /locus_tag="VFV20_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEU5288859.1"
                     /translation="MLEIPGQVWQRVKTGPLWRSLFRHGYPDSRKNQSLAVFTNVFLH
                     LHPVKVRRHALAIPYTWCMGGLSFFLFLVLTLTGTLLMFYYRPTTEWAYSDIKDLETV
                     VVFGQLLRNMHRWAAHGMVITVFLHMVRVFYTGSYKPPREFNWVVGTLLFLFTILLSY
                     TGYLL"
BASE COUNT          520 a         1109 c         1065 g          520 t
ORIGIN      
        1 gtcatcgccg cgctgtgggc cctcttcgcg cgcatccgtc gcgcgcgacg acccccgcgg
       61 cggttgatcg tcgtcccgct cgcggcgttc ggcctcttcg tgcttcttct gttcctgctc
      121 gagctcgtgc gctgagcctc agcgtccacg cagggctgcg gccgtctcgc gctcgacctc
      181 acggatgacc atccgcgggt tcgcgatgag cgactcgagc gccttcgcga tggcgagccg
      241 gtagggcggg gcgaggtcat cagggagatc gtcctcgtcg aggaccgtcg gctcgcccga
      301 cgtccggatg aggacgtcga ccgtgaggtc gcggtaggca acgagcgccg gaacgatcgt
      361 cgtgtccgtg gccacgttgc agtagtaggc gagcgtgcgg ccgcccacga gccagtggta
      421 cacgttgtac ggccggtcga cccagtagtg gccgaccgtg accgtgcccg ccggcacgcg
      481 gatatccgac ccggaaatgg cccattcgcg gtcggtcacg tacctgagga ccgcccgccg
      541 gcccggctcc acgacgatcg cctcgcaggc gaactcggcg accgatccgt caagacgggt
      601 cttgcgctca gttatggccg ctgtggtttg actgggcact acgcgtcggt agcatgaccg
      661 acaccgcatg gacatgaaca cgaggtagcg cgtgagagag ctctaccaag tcgtggaggt
      721 gggccctgcc tggtatcagg agaacatccc ctgccaggag gcctgtcccg tacgcacgaa
      781 ttgccggggg tatctgaatc tcgccgcggc cggcgagttc gagaaggcgt gggagcttgc
      841 cctcgacccc aacccgatgg cctccatctg cggctcggtc tgcgccgccc cctgcgagac
      901 ggcctgccgc cgcaaggagg tcgataagcc gctctccatc cggtacatga agaagttcct
      961 ctcggactgg acgcaccaga acatcaccgt gaacgggcgc gcctacacgc agcccgcgcc
     1021 gccggtgttc ggtccgccgc gcgggaaggt ggcgatcgtc ggagcggggt gcgccggcct
     1081 tggcgccgcg agcgacctcg cgaagctcgg gttcggcgtc accgtgctcg acgcgctcga
     1141 tcaggcgggc ggcaccgcgc tcgcgggcgt acccccgttc cgtctcccgc gcgaggtcat
     1201 cgaccgcgac atcaacggca tcgtcaacga gaacgtcgag ctgcggctct ccacgtacgt
     1261 cggtcgcgac gtcacgctgc ggcagctcca cgacgagtac gacgccgtgc tcgtgaccgc
     1321 gggctgcttc gagccgaact atccgggcat ccccggcgag gacgccgacg gcgtcatggc
     1381 cggcatcgtg ttcctggagc gcgcgtaccg cggacgtccg ctcgaggtgg ggaagcgcgt
     1441 ggtcgtgctc ggcggtggct acaccgcgat ggactgcgcg tcgacctcct ggcgtctcgg
     1501 cgcggacgac gtgtacatcg tgtaccgccg ctcgcgggac gagatggtgg tcgacgagga
     1561 ggagctcggc gagaccgagc gcgagggcat gcggttcgtc tacctcgcct cgcccgtcgc
     1621 catcctcacg aacgacgccg gccacgtccg cggcgtgcgg ttcatccgca acaagctcgg
     1681 cgagcccgac aagagcggcc gccgcagccc ggagccgatc gagggctccg agttcgacat
     1741 cgagtgcgac accgtgctcc tcgcgctcgg tcaggcgccg gacacgacct tcctcgcgga
     1801 gttcccggag ttccgcgtga agcgctggcg cgacgtgaag ttggacgccg acacgtacca
     1861 gacggtggtg ccgaaggtct tcatggccgg cgactaccgc accggtccga cgacgatcat
     1921 cgaggccgtc gccgagggcc ggtcggccgc gcagcagatc gcgcgcttcc tggacccgct
     1981 cgcggtcgcg gtgaacatcc ccgagtacca cgagatcgtg acgctgcgcc gctcgagcac
     2041 gccggacaac gtcgggcaga tcgccggcga gggcgagctc gcgcgcatct accacaacat
     2101 gtccgtcgac tacgaccgca tcccgcggca ggagatgccg aagctcgcga agaaggagcg
     2161 ccgcgggctt ttcctcgagg tgaaccaggg ttacagcgaa gcccaggcgg tcgccgaagg
     2221 cgatcgttgc ctcaagtgca acttcaacat cgagatcatc ggcgagctct gcatcatctg
     2281 cggcggatgc gtcgatgtct gccccatgga cgtcatccac atggtcgaca tgagcgacat
     2341 cgtcgacgac ggtgccatcc ccggcgtggg cgaggcgaag aagtgggacc gcggcgtcgc
     2401 gttcttcctc gacgagacgg cctgcatccg gtgcgcgctc tgcgtgatcc gctgccccac
     2461 ggacgcgatc acgatgaacc gctacggcgc ggtcatgccc acgatcggaa agacgctgcc
     2521 gaaggaatcg atcgacgacg agatccacct cgatctcacc gtcgcgtcac gaaagtcgat
     2581 ccggccgctg ccgatgtacg acccggctct ggccgccggc tacaagccgg cgtcgaacgg
     2641 tcacgcgaac ggcaacggcg cgaacggcca caatggcaag ccggcgcggg agaaggaacg
     2701 ggaggcggtg cactaaccca tgctcgagat ccctggacag gtctggcagc gcgtgaagac
     2761 gggaccgctc tggcggtcgc tcttccgtca cggctatccc gactcccgca agaaccagtc
     2821 gctcgcggtg ttcacgaacg tcttcctgca cctgcatccc gtgaaggtcc gccggcacgc
     2881 gctcgcgatc ccctacacgt ggtgcatggg cgggctgtcg ttcttcctct tcctggtgct
     2941 gacgctcacc gggacgctgc tgatgttcta ctaccggccc accaccgaat gggcgtactc
     3001 ggacatcaag gacctcgaga ccgtcgtcgt gttcggacag ctcctgcgga acatgcaccg
     3061 ctgggccgcg cacggcatgg tcatcaccgt gttcctccac atggtccgcg tcttctacac
     3121 cggctcgtac aagcccccgc gcgagttcaa ctgggtcgtg ggcacgctgc tcttcctctt
     3181 cacgatcctg ctgtcgtaca ccggctacct gctg
//
LOCUS       DASTZR010000479         2710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_652724, whole genome shotgun sequence.
ACCESSION   DASTZR010000479 DASTZR010000000
VERSION     DASTZR010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652724"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VFV20_10685"
     CDS             <1..239
                     /locus_tag="VFV20_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEU5288860.1"
                     /translation="QVLDRDGVGEMMRISIQKGRKTRKDLKIGICGEHGGDPSSIEFC
                     HQVGLTYVSPSTYRVPVARLAAAQAALAMGEKDR"
     gene            298..1410
                     /locus_tag="VFV20_10690"
     CDS             298..1410
                     /locus_tag="VFV20_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009053022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEU5288861.1"
                     /translation="MATNSRVTTSGAVERDAKLSRTKSVASLRIRERTEELEANDLAP
                     HATKSATAKRARPEEASPTRTAFALDRDRVVHSKAFRRTKHKTQVFLAPVGDHYRTRL
                     THILEVSQIGRAMARALRLNEDLVEAMALGHDIGHTPFGHIGEELLARFLPEGFRHNQ
                     QSVRIAERLEKNGAGLNLTHETLDGILKHSAPTTGGVETAAWGEPETPEGWVVRYADK
                     IAYLHHDIDDAMRAGITTEADLPIEVRVSLGRDRAERLDTMIYDVIVTSYGKPQVTMS
                     QEVLEATNALRKFMFEHVYLAGPAKTEDEKAQGVLWELLQYFERHPDRMPQEHQRIAE
                     RDGTKRAVADYIAGMTDRYALDTYTSLFIPSAWGAL"
     gene            1473..>2710
                     /gene="dnaG"
                     /locus_tag="VFV20_10695"
     CDS             1473..>2710
                     /gene="dnaG"
                     /locus_tag="VFV20_10695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU5288862.1"
                     /translation="MPGDFDTVKERIDIVALIGEKVPLKRAGKVYKGLCPFHVEKTPS
                     FQVDPERRNYKCFGCGEGGDCFTWLEKMENLEPVEALKVLAERAGVELTRRAPEERER
                     ETRLLTVNEKAHFYFRQALRGTDKGKAAAAYIAKRGITSETIDKFGLGYAPDSFNGLL
                     GYLKKHGFSEEEGLAAGVLTQTDRGTFDRFRDRLMVPIRDQKGRAIAFGGRAMRSDQQ
                     AKYVNSTSTLLFNKSDTLYAFDVARSAIRTEGTAVIVEGYFDAIACHQAGIANVVASM
                     GTALTPEQYLRLQDMKIERVVVAFDGDAAGQRSAERRGRDLLPLLQRHFRKAGTGQVG
                     TRGFATLYVTVLPEGKDPDDLARSDPAHLRRLIAEAASLLEFLIAKIRERSRLDRPEG
                     QLRFLDEAAELLAAEPDQIRR"
BASE COUNT          476 a          917 c          963 g          354 t
ORIGIN      
        1 tccaggtgct cgaccgcgac ggcgtggggg agatgatgcg gatctcgatc cagaagggcc
       61 gcaagacgcg gaaggatctc aagatcggca tctgcggcga gcacggcggc gacccgtcgt
      121 cgatcgagtt ctgccaccag gtggggctga cgtacgtctc gccgtccacg tatcgggtgc
      181 cggtcgcgcg gctcgccgcc gcgcaggcgg cgctcgcgat gggggagaag gaccgctaac
      241 gaccggcgcg gacctcgccg gcgctgcgcc ggcggctgcc gattaggcta gtgcccgatg
      301 gcgaccaact cgcgcgtcac gacgagcggt gcggtggagc gcgacgccaa gctctcgcgc
      361 acgaagagcg tggcgtcact tcgcatccgc gagcggacgg aagaactcga ggcgaacgac
      421 ctcgccccgc acgcgacgaa gagcgcgacg gcgaagcgcg ctcggcccga ggaggcgtcg
      481 cccacgcgga ccgcgttcgc gctggaccgc gaccgcgtcg tccacagcaa ggcgttccgc
      541 cgcacgaagc acaagacgca ggtcttcctc gcgccggtgg gcgatcacta ccggacacgg
      601 ctgacgcaca tcctcgaggt gagccagatc ggccgcgcca tggcgcgagc gctgcgcctg
      661 aacgaggacc tggtcgaagc gatggcgctc ggccacgaca tcggccacac gccgttcggc
      721 cacatcggcg aggagctgct cgcgaggttc ctgcctgagg gcttccgcca caaccagcag
      781 agcgtgcgca tcgccgagcg gctcgagaag aacggcgcgg ggctgaacct cacgcacgag
      841 acgctcgacg gcatcctgaa gcacagcgcg ccgaccaccg gaggcgtcga gaccgccgcc
      901 tggggcgagc ccgagacgcc ggagggctgg gtggtgcgct acgccgacaa gatcgcctac
      961 ctgcaccacg acatcgacga cgcgatgcgc gcgggcatca cgaccgaggc cgaccttccc
     1021 atcgaggtgc gcgtctcgct ggggcgcgac cgggccgagc ggctcgacac gatgatctac
     1081 gacgtgatcg tcacctcata cgggaagccg caggtcacga tgagccagga ggtcctggag
     1141 gccacgaacg cgctgcgcaa gttcatgttc gagcacgtat acctcgccgg tcccgcgaag
     1201 acggaggatg agaaggcgca gggcgtcctg tgggagctgc tccagtactt cgagcggcat
     1261 cccgaccgca tgccgcagga gcaccagcgg atcgccgagc gcgacgggac caagcgagcc
     1321 gtggccgatt acatcgcggg gatgaccgac cgctatgccc tcgacacgta caccagcctc
     1381 ttcatcccgt cggcctgggg cgcgctgtag cgagctcgtc ggccggcgca gcaaaagtca
     1441 tcgccgctcg ttcttgttga ggggggacac gcgtgccggg tgacttcgac acggtcaagg
     1501 agcggatcga catcgtcgcc ctcatcggcg agaaggtgcc gctgaagcgc gcgggcaagg
     1561 tctacaaggg cctctgcccc ttccacgtcg agaagacgcc gtccttccag gtggacccgg
     1621 aacgtcgcaa ctacaagtgc ttcgggtgcg gcgaaggcgg ggactgcttc acctggctcg
     1681 agaagatgga gaacctcgag ccggtcgagg cgctgaaggt gctcgccgag cgcgcgggcg
     1741 tcgagctcac gcggcgcgcg cccgaggagc gcgagcgcga gacgcgcctg ctgacggtga
     1801 acgagaaggc gcacttctac ttccgccagg cgctgcgcgg gaccgacaag ggcaaggccg
     1861 ccgccgcata catcgccaag cgggggatca cgtccgagac gatcgacaag ttcggtctcg
     1921 gctacgcgcc cgacagcttc aacgggctgc ttggctacct gaagaagcac gggttcagcg
     1981 aggaggaggg ccttgccgcg ggggtgctga cgcagaccga ccgcggcacg ttcgaccgct
     2041 tccgcgaccg gctgatggtc ccgatccgcg accagaaggg cagggccatc gcgttcggcg
     2101 gccgcgcgat gcggtcggat cagcaggcga agtacgtgaa ctccacgagc acgctgctct
     2161 tcaacaagag cgacacgctc tacgccttcg acgtcgcgcg ctcggcgata cggacggagg
     2221 gcaccgcggt catcgtcgag gggtacttcg acgcgatcgc ctgtcaccag gcggggatcg
     2281 cgaacgtcgt cgcgtcgatg ggtaccgcgc tcacgcccga gcagtacctg cggctgcagg
     2341 acatgaagat cgagcgcgtg gtcgtcgcct tcgacggcga cgccgcgggc cagcgctccg
     2401 ccgagcgtcg cgggcgcgac ctcctgccgc tgctccagcg ccacttccgg aaggccggta
     2461 ccggccaggt cgggacgcgc ggattcgcga cgctgtacgt gacggtgctg ccggagggca
     2521 aggatccgga cgatctcgcg cggagcgacc cggcacacct gcggcgtctc atcgcggagg
     2581 ccgcgagcct gctcgagttc ctcatcgcca agatccgcga gcggtcccgg ctggaccgtc
     2641 ccgaagggca gctgcgcttc ctcgacgagg cggcggagct cctcgccgcc gagccggacc
     2701 agatccggcg
//
LOCUS       DASTZR010000480         4246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_385879, whole genome shotgun sequence.
ACCESSION   DASTZR010000480 DASTZR010000000
VERSION     DASTZR010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385879"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..647)
                     /locus_tag="VFV20_10700"
     CDS             complement(120..647)
                     /locus_tag="VFV20_10700"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEU5288863.1"
                     /translation="MASRIRFSAAMSLDGYLAGPAGEADWIVPDPETDFSALMAQFDT
                     LLVGRRTFETMVRAKRTTMPGMRTVVLSTTLRQVDYPDVTIVGDEVEAAVKSLRANSR
                     KDVWLFGGGELFRRLLDAGLVDSVELAVEPILLGGGIPLVREALHRHQLRLAAHRVSK
                     VGVVHLEYVVTHPAA"
     gene            complement(848..1285)
                     /locus_tag="VFV20_10705"
     CDS             complement(848..1285)
                     /locus_tag="VFV20_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288864.1"
                     /translation="MPETILAESRYRAITAQAWSAMLAVLLTMLIADVDRLAIQGSYA
                     ELAESLGADPGPRGLWVLVCLICLNALVQIAVRTFDAPPFRRVVFWTSVAYTVFFILH
                     QAAHVAAGERFSLHTILDLTHHILGLWACWASGRWSRLARARG"
     gene            complement(1549..4246)
                     /locus_tag="VFV20_10710"
                     /pseudo
     CDS             complement(1549..4246)
                     /locus_tag="VFV20_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721720.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
BASE COUNT          694 a         1398 c         1364 g          790 t
ORIGIN      
        1 gagggggcgg gctcggaacc gtgagcgagc cccgatctga cgagttggtt ggctggcgcc
       61 tcgcggacct gcgcttcggc cgcgcagccc gcacgcgaaa tggtagcgca ctcgcgcgct
      121 taggcagccg ggtgcgtgac gacgtattcg agatgaacga cgccgacctt tgacacgcga
      181 tgagcggcga gccggagttg gtgccgatgc aaggcctcgc gcaccagagg aatcccgccg
      241 ccgagaagaa tcggctcgac ggccagttcg acgctgtcca ccagtccggc gtccagcagc
      301 cttcgaaaca actcgccgcc cccgaagagc cacacatcct tccgagagtt ggcgcgcagg
      361 cttttcacgg cagcctcgac ctcatctcca acgattgtga cgtcgggata gtcgacctgc
      421 cgaagcgtcg ttgataagac gaccgtgcgc atccccggca tggtcgttcg tttggcgcga
      481 accatcgtct caaacgttcg gcgaccgacg agcagcgtat cgaattgggc catcagcgcg
      541 gagaagtccg tctccggatc cggcacgatc caatcggcct ctccggccgg accggcgagg
      601 tacccgtcca gactcatggc ggcgctgaat cggattcgtg atgccacgca agagctccgc
      661 ttttgtggcc ggcctgacgg acctgcgctt catccgcgcg gaccgcacgc gaaatggtag
      721 cgcactcgcg agaacgtcgc cgcgggctgc gtcggctgca agcgcttgtt aggtcgcagt
      781 gtgcactacg acggtttcga cagcagcgga tggagagggg atagtttcgc gttgagccag
      841 cgccacacta accacgtgcc cgtgcgagcc ttgaccacct accggaagcc caacacgccc
      901 acagtccgag aatgtgatga gtaaggtcga ggatggtgtg aaggctgaac cgttcgcccg
      961 ccgcgacgtg cgccgcctgg tgcaggataa agaacaccgt gtacgcgacg ctcgtccaga
     1021 acaccactct acgaaagggc ggcgcatcga acgtgcgcac cgcgatctgc acgagcgcgt
     1081 tcaggcagat gaggcagacc agcacccaca ggcccctcgg gcccggatcg gcaccgagcg
     1141 actctgcgag ctcggcatag gacccctgaa tcgcaagtcg gtccacgtcg gcaatgagca
     1201 tggttagcag caccgcgagc atcgccgacc atgcctgggc tgtgattgcg cgataccggg
     1261 actctgcgag gatcgtttca ggcacaatga ctctcccttg aatctgtatt ggttcagccg
     1321 tgccgagtgt cgcctggcta gactctaagc tgcttggacc gcagcggcct aaaaatctcc
     1381 tcagtcgtga ttcgcccctc tccccagccc tatccccgcg acgggaagag ggagcaggtt
     1441 ctcgcataat tcgcgatcac tgtccgaaag aaaaagggcg gcccgtcagg gccgccctcg
     1501 atctgcaatc cgcgatcagc gatctgcaat cgcgcgcgaa gcgcgcgcct actgttcctc
     1561 gtcgaagtcc gccagcgcga gcgcttcctc gtcttcggcg cggagcgcgt cctcgagggt
     1621 ctgcggcgcg ggctcttcct tctccatgta cagctcgttg tactgcggca tgcccgttcc
     1681 cgccgggatg agccgcccga cgatgacgtt ctccttgagg ccgcgcagat agtccacgcg
     1741 tcccgcaata gaggcttccg tcagaaccct cgtcgtctcc tggaacgaag ccgccgagat
     1801 gaacgagtcc gtcgagagcg atgccttggt gatgccgagc aggagcggaa cgccgtccgc
     1861 cggctcgccg ccgcgggcga tgaccttctc gttctcttcc atgaaccgga agcgatcgac
     1921 ctgctcgtcc accaggaagt ccgtatcgcc cacgcgcgtc acgcgcaccg agcgcatcat
     1981 ctggcggacg atgacctcga tgtgcttgtc gttgatcgcg accgactgcg agcggtagac
     2041 ctcctggatc ttgtccacca ggtagcgctg cagttccttc acgccgagga ccgcgaggac
     2101 gtcgtgcgga tcgatcggac cgtcgatcag aggatcgccg gcgcgaaccc gctcgccttc
     2161 ctgcacgttg acgtgggtgt accgcggcac cgagtactcg taccgcgccc cctcgtccgt
     2221 ctcgacgacg atcttccgca tgcccttcac caccgggccg tgatgcacca gaccgtcgac
     2281 ttcggtgatg accgccttct cccgcggatg gcgcgcctcg aagagctcga ccacgcgcgg
     2341 cagaccgccc gtgatgtcct tcgtcttggt cgtcgcgcgc gggatcttcg cgatgatgtc
     2401 gcccgggaag acctcctggc cgttggtgac catgaggtgc gcctggaccg gaaggaggta
     2461 cttcttctcc tcccccttct tccccttgac cgtgatcgtc ggggtccgct tctcgctggc
     2521 cggcgactcg acgatgacca tctgggtgag gcccgtgacg cggtccgtct cctcgcggat
     2581 gttctcgcct tcgatgatgt ccttgaagga ggcctttccg ccgacctcgg tgaggatcgc
     2641 cgaggtgaac ggatcccact cgacgagggc gtcacccggc tcgaccacct gaccttcgcg
     2701 aaccttcagc gtcgatccgt acacgaccgc gtaccgttcc ttctcgcggt tgtgctcgtc
     2761 gacgatgagg agctttccgt tccggttgat gacgacgagg ttgccgtcct tgttggtgac
     2821 cgtcgcgacg ttcaggaact gcaccgttcc ggggttcgtc gcctcgtgct tcgactgctc
     2881 ggagacgcgc gacgcggttc caccgacgtg gaaggtccgc atcgtgagct gcgtgcccgg
     2941 ctcgccgatc gactgcggcg atgacgccga ccgcttcgcc gatctcgacc agccggccgg
     3001 tggccaggtt gcggccgtag cagcgccggc agacgccgcg ccgggtctcg caggtgagca
     3061 ccgagcggat gcgcatctgc tcgatgcccg cttcctggat ctcgttcgcg agatcctcgg
     3121 agatctcgtc gttctggctg acgagcttcg cgccgttgag cggatcgaag acgtcttcct
     3181 gcgcgacgcg gccgacgatg cgatcgcgga gcggctcgag gatgtcgccg ccctcggtga
     3241 tcgcgctgac cgtgatgccg tcgaaggtgt ggcagtcctg ctcggtgacg atgacgtcct
     3301 gcgccacgtc gaccagacgc cgcgtgaggt agccggagtc cgccgtcttg agcgccgtgt
     3361 ccgcgaggcc cttgcgggcg ccgtgcgtcg agatgaagta ctggaggacc gagaggccct
     3421 cgcggaagtt cgccgtgatc ggggtctcga tgacctcgcc cgagggcttg gacatcaggc
     3481 cgcgcatgcc ggcgagctgg cgcacctgct ccttggagcc tcgcgcgccc gagtcggcca
     3541 tcatgaagat ggggttgaac tcgtggcggt cgatctccag ccgcttcatg tcggagaaca
     3601 tctcgtcgga cacctgctcg gtcgcgcggt gccagatgtc gatgatcttg ttgtggcgct
     3661 cgccgtgcgt gatcgcgccg tcgagccgct gcttctcgac ctcgagaacg ttcttgcgcg
     3721 cgttctcgac gatctcgttc ttccgggccg ggatgaccat gtcgtcgacg ccgatggaga
     3781 cgcccgcgag cgtcgcccag gtgaagccga ggtccttgag gccgtcgagc atcttgaccg
     3841 tcaggtcgtt gccgtaggtg atgaagcaga agttgacgag ctcctgcagg cccttcttct
     3901 tgaggagacc gttgatgtag gggatctccc gcggaaggtg catgttgaag atgacgcgcc
     3961 cgaccgtggt ctcgatgagc tcgttctcga cgtgctggac gtcggtgtgc aggatgtcct
     4021 ggtcgtcgta caccgtggtg aggttgatgt actcgcccga gtagcgcatg cggatgggcg
     4081 acagcgtctc gacctcgccc gccgccagcg ccaggtacac ctcctccggc gagccgagca
     4141 gcacctcccc cgcgtcgaag gccatgcggg cctcggcgac cgaggagaac acgcgtccgg
     4201 cgcccttgcc gtcctcgacc tcggtggtga ggtggtagag gccgat
//
LOCUS       DASTZR010000481         2568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_730619, whole genome shotgun sequence.
ACCESSION   DASTZR010000481 DASTZR010000000
VERSION     DASTZR010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730619"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="VFV20_10715"
     CDS             <1..1134
                     /locus_tag="VFV20_10715"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEU5288865.1"
                     /translation="DDAETIVVAMGTIADTAIAVVDALRAAGRRVGCVGVTSYRPFPA
                     ARLAAVLRNARAVGIIERTDEPAAADNPLTRETKAALSDLASDGARIPRVFSASAGLG
                     SRDVAAGDIAAVYRWLEEPATAPRRFAVLGVKHPLALERLAIDVRPRGAYSLRGHSIG
                     GFGSVTTNKLLAALAGDMFGHHVQAYPRYGSEKKGLPTTYFLTIAEERIRTHAELDQV
                     DFVPLHDVSAFGHGDPLRGLVDGGVLFLQSTLTDANAIWKSIPAEARTRILARHIRVA
                     AMDTAGLARRHSPRPDLLVRMQGVALVGVFLRLTPFAARAKLSGEPLMDAVRDRLTRF
                     FGKRGERVVDANLRVIREAFDGVIDVTAAIQPGAEPRAKEVVA"
     gene            1167..1550
                     /locus_tag="VFV20_10720"
     CDS             1167..1550
                     /locus_tag="VFV20_10720"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5288866.1"
                     /translation="MTRGLVTVRASARLYEAAQLMDAHKIHGLPVVDSSGAVVGVLSQ
                     TDLLRARVVEHLWTNLRGLAVRHVMTAPAITVALHATLDEAAELMEQRRVHRLVVVGS
                     DGTTPVGILSVSDLVHVMAAERTEH"
     gene            1550..>2568
                     /locus_tag="VFV20_10725"
     CDS             1550..>2568
                     /locus_tag="VFV20_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEU5288867.1"
                     /translation="MTLTKEPGAAMETAQFGPVEHLLDVHQYVDDFNDAVIGAYAANT
                     SDEALAADVGVARSIIPPGTAATRDFSHLAPQIPEFIADRCVGCMSCVNACPDTAILA
                     VALGKSKVAERIETFAQSEPVAARARETAQAHFADTRKYGEVPAKKGHEPAAFGIFVD
                     PVHCKGCGECVEVCVALGYDALRMVDKVPGGRNGEATLTHYDRDMRFFRTLPPTPPEY
                     RNEKALADLMLGESAIGYVGGAGSCSGCGEATAIRMLVAATRQVYGPEGMGIVASTGC
                     NTVFGSTYPYNPYLVPWTNSLFENAPADAMGIRARWDQAGHPDRRLWVIGGDGAMYDI
                     GFQSLS"
BASE COUNT          368 a          920 c          925 g          355 t
ORIGIN      
        1 gatgacgctg agacgatcgt cgtggcgatg ggcacgatcg ccgacacggc catcgcggtc
       61 gtcgatgccc tccgcgcggc cggccggcgt gtcgggtgcg tcggcgtgac gtcgtaccgg
      121 ccgttcccgg ccgcgcgcct cgcggccgtg ctgcgcaacg cgcgggccgt cgggatcatc
      181 gagcgcaccg acgagcccgc ggccgccgac aacccgctca cccgcgagac gaaggcggcg
      241 ctctccgacc tcgcctccga cggcgcgcgc atcccgcgcg tgttctcggc gagcgcgggc
      301 ctgggctcgc gtgacgtcgc cgcgggcgac atcgcggccg tgtaccgctg gctcgaggag
      361 ccggcgaccg cgccgcgccg cttcgcggtg ctcggcgtga agcacccact ggcactggaa
      421 cggctcgcga tcgacgtgcg cccgcggggc gcgtacagcc tgcgcggcca ctcgatcggt
      481 gggttcggtt cggtcaccac gaacaagctg ctcgcggcgc tggccggcga catgttcggc
      541 caccacgtgc aggcgtatcc gcggtacggc tccgagaaga agggcctgcc caccacgtac
      601 ttcctcacga tcgcggagga gcggatccgc acccacgccg agctcgacca ggtcgacttc
      661 gtcccgctgc acgacgtctc ggccttcggc cacggcgacc cgctgcgcgg gctggtcgac
      721 ggcggcgtcc tgttcctgca gagcacgctc accgacgcga acgcgatctg gaagtcgatc
      781 cccgccgagg cgcgcacgcg catcctcgcg cgtcacatcc gcgtcgcggc catggacacc
      841 gccggcctcg cgcggcgcca ttcaccgcgg cccgatctcc tggtgcgcat gcagggtgtc
      901 gcgctcgtcg gcgtgttcct gcgcctcacg ccgttcgcgg cgcgcgcgaa gctctccggc
      961 gagccgctca tggatgcggt gcgcgaccgg ctgacgcgct tcttcggcaa gcgcggcgaa
     1021 cgcgtggtgg acgcgaacct cagggtcatc cgcgaggcct ttgacggcgt gatcgacgtg
     1081 acggcggcga tacagcccgg ggcagagccg cgggcgaaag aggtggtcgc atgaaagctg
     1141 tggcggcgcg aacggtgggc gaggcgatga cgcgagggct ggtcacggtg cgcgcgagcg
     1201 cgcgcctcta cgaggcagcg cagctcatgg acgcgcacaa gatccacggc ctgccggtgg
     1261 tcgactccag cggagcggtg gtcggcgtcc tgagccagac cgacctcctg cgcgcgcgcg
     1321 tcgtggaaca cctgtggacg aacctgcgag ggctcgcggt gcggcacgtc atgaccgcgc
     1381 cggccatcac ggtggcgctg cacgccacgc tcgacgaggc cgcggagctc atggagcagc
     1441 ggcgcgtgca ccggctcgtt gtcgtcggat ccgacggcac gacgccggtc ggcatcctgt
     1501 cggtgtccga cctcgtacac gtcatggccg cggagaggac cgaacactga tgacgctcac
     1561 gaaggaaccg ggcgcggcga tggagaccgc gcagttcggt cccgtcgagc acctgctcga
     1621 cgtgcaccag tacgtcgacg acttcaacga cgcggtcatc ggcgcctacg cggcgaacac
     1681 ctcggacgag gcgctggcgg ccgacgtggg cgtcgcccgc agcatcatcc cgcccggcac
     1741 ggcggccacg cgtgacttca gccacctcgc gccgcagatc ccggagttca tcgcggaccg
     1801 gtgcgtcggc tgcatgtcgt gcgtgaacgc gtgcccggac acggcgatcc tcgcggtggc
     1861 gctcgggaag tcgaaggtcg ccgagcgcat cgagacgttc gcgcagtcgg agccggtcgc
     1921 ggcccgcgcg cgcgagaccg cgcaggcgca cttcgccgac acgcgcaagt acggcgaggt
     1981 gcccgccaag aagggccacg agccggcggc gttcggcatc ttcgtcgacc ctgtgcactg
     2041 caagggctgc ggcgagtgcg tcgaggtgtg cgtcgccctc ggctacgacg cgctgcgcat
     2101 ggtcgacaag gtcccaggcg gccggaacgg tgaggcgacg ctgacccact acgaccgtga
     2161 catgcgcttc ttccgcacgc tcccgccgac gccgccggag taccggaacg agaaggcgct
     2221 cgcggacctc atgctcggcg agtcggccat cggctacgtc ggcggcgcgg gctcgtgctc
     2281 gggctgcggc gaggcgaccg cgatccggat gctcgtggcc gccacgcgcc aggtgtacgg
     2341 acccgagggc atgggcatcg tcgcgtcgac gggctgcaac acggtcttcg gcagcacgta
     2401 tccgtacaac ccgtacctcg tcccgtggac gaactcgctc ttcgagaacg cgcccgccga
     2461 tgcgatgggt atccgcgccc gctgggatca ggccggacac cccgaccgcc gtctctgggt
     2521 gatcggcggc gacggcgcga tgtacgacat cgggttccag tcgctgtc
//
LOCUS       DASTZR010000482         4065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_103474, whole genome shotgun sequence.
ACCESSION   DASTZR010000482 DASTZR010000000
VERSION     DASTZR010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103474"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VFV20_10730"
     CDS             complement(<1..457)
                     /locus_tag="VFV20_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010277366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HEU5288868.1"
                     /translation="MTTPYELVIGIETHVELRTRSKMHCGCSASWFGAPANSLVCPVC
                     LGLPGALPVPNRRAIELAITAGLALNCEVPEHTKFDRKNYMYPDLPKGYQISQYDLPF
                     NVNGWIEIPSGEGTKRVRIHRAHLEEDTANTKHGEGYALIDFNRSGVPLL"
     gene            complement(454..1068)
                     /locus_tag="VFV20_10735"
     CDS             complement(454..1068)
                     /locus_tag="VFV20_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288869.1"
                     /translation="MGTRHEDQPAEHWAGPESLDPTPVWKQYALVALLLVVGLVVLVI
                     VAVAALAPQLATPPALVPGDRLVLAASDVPAVGARPRLVASPVIDDARSFYLVQPRKG
                     DVLALRAHWARRPGEPECRVAVLAVREGAELGAIDCPVFRPESATFDLLGNPATPGAI
                     RGLDQYLVSVSGDRVIVNLSRVIRADERTSAPVPTGIPQPQQTP"
     gene            complement(1069..2226)
                     /locus_tag="VFV20_10740"
     CDS             complement(1069..2226)
                     /locus_tag="VFV20_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5288870.1"
                     /translation="MRPLTNTRVSKIPASGIRRFFDLIAGVEGAISLGVGEPDFITPE
                     PFREAAIRSIRDGRTKYTSNYGIKPLRVAISEHTAKLRGVRYDPDREILVTVGVSEAV
                     DLALRATLNDGDEVILADPSYVAYVPGIVLAGGVPVPVPTEESEEFRLTPAAVQKAIT
                     PRTRAILLGFPNNPTGGVLRREDVEGIARLAQEHDLLVYSDEIYDRLVYGVEHHSIAS
                     IPEMKQRTVYLAGFSKSYAMTGWRVGYACAPAEIVEQMMKIHQYTVMCVPTAAQYAAL
                     EALRVGEPEVQRMVKAYDERRQLMWRRFNELGLRCFEPRGAFYCFPNITSTGLSDDEF
                     CERLLKEEKVVVVPGNAFGERGKGHVRACYAASLENIGEACDRIERFIARL"
     gene            complement(2238..3689)
                     /gene="gatA"
                     /locus_tag="VFV20_10745"
     CDS             complement(2238..3689)
                     /gene="gatA"
                     /locus_tag="VFV20_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEU5288871.1"
                     /translation="MNALPRSITEAAALLRRREASAAEVTEAALARAHGDRYNAWLLV
                     ADDHARAQAKAADERLRRGDAPLLAGIPWACKDIIGTKGIETTAASKILKGYVPPYSA
                     TVVERLDAEGAVTIGKTNLDEFAMGSSNDNSAFGAVQNPWDTARVPGGSSGGSAVAVA
                     AGQALFALGTDTGGSIRQPGALTGVVGMKPTYGRVSRFGVVAFASSLDQVGPFTNTVE
                     DCAIVCQALFGQDPNDATTMPLATEDLRRELDKGVKGLRIGVPKEFFIEGIETGVERA
                     VREALRWYEKEGAELVDVSLSLTDHGLAVYYIIQPAEASSNLARYDGVRFGLRVEGKD
                     LVETYRRTRGEGFGAEVKRRMILGTYALSAGYYDAYYVKAQKVRTLIKAEFDTAFERC
                     DAIVTPTSPTVAFKIGQKVNDPLAMYLNDVFTLPGNIAGLPGMSIPCGLSEGLPVGLQ
                     VMANSFQEATMFRVAAAYERATKHHEMRPQEAA"
     gene            complement(3686..3985)
                     /gene="gatC"
                     /locus_tag="VFV20_10750"
     CDS             complement(3686..3985)
                     /gene="gatC"
                     /locus_tag="VFV20_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU5288872.1"
                     /translation="MPIDRQTIVHVADLARIRLSDEETLRFTGQLSVVLDAVARLGEV
                     DTSAVLPSASVLPLQDVQRDDEARPGLTNAQALANAPKGGRDGEFFRVQEVLEER"
BASE COUNT          592 a         1398 c         1427 g          648 t
ORIGIN      
        1 cgagcagcgg cacgccggag cggttgaagt cgatgagcgc gtagccctcg ccgtgcttgg
       61 tgttcgccgt gtcctcctcg agatgggcgc ggtggatgcg cacacgcttc gtgccctcgc
      121 cgctggggat ctcgatccag ccattcacgt tgaacggaag gtcgtattgc gagatctggt
      181 agcccttcgg caggtccggg tacatgtagt tcttgcggtc gaacttggtg tgctccggga
      241 cctcgcagtt gagggcgagg ccggcggtga tcgcgagctc gatggcccgg cggttcggca
      301 ccgggagcgc gccgggcagg ccgaggcaga ccggacagac cagcgagttc gccggcgctc
      361 cgaaccagct cgcgctgcac ccgcagtgca tcttcgagcg tgtccggagc tcgacgtgcg
      421 tctcgatgcc gatgacgagc tcgtacggcg tggtcatggc gtctgctgcg gctgcgggat
      481 gcccgtcggc accggcgcgc tcgtccgctc gtcggccctg atgacgcgcg agagattcac
      541 gatgacgcga tcgccgctca cggacacgag gtactggtcg agcccgcgta tcgcgcccgg
      601 agtcgccgga ttgccgagaa gatcgaaggt cgcggactct ggccggaaga ccgggcagtc
      661 gatcgcgccg agctccgcgc cctcgcgcac cgccaggacg gcgactcgac attccggctc
      721 gccgggccgg cgtgcccagt gcgctcggag cgccaagacg tcaccctttc gcggctggac
      781 caggtagaag gagcgagcgt cgtcgatcac cggagacgca acgagacgcg gacgtgcgcc
      841 gaccgccggc acgtcgctgg cggcgagcac gaggcgatca cccggcacga gcgcgggcgg
      901 cgtcgcgagc tgcggcgcga gcgccgcgac cgcaacgatc acgagcacga ccagaccgac
      961 gacgaggagc agcgcgacga gtgcgtactg cttccacacc ggcgtcggat cgagcgactc
     1021 cggacctgcc cagtgttccg ccggctgatc ctcatgacgc gttcccatct acaggcgggc
     1081 gatgaaccgc tcgatgcggt cgcacgcttc gccgatgttc tcgagagagg ccgcgtagca
     1141 ggcgcggacg tgtcccttgc cgcgctcgcc gaaggcgttg ccggggacca cgacgacctt
     1201 ctcctccttg agcagccgct cgcagaactc gtcgtcggag agcccggtcg aggtgatgtt
     1261 cgggaagcag tagaaggcgc cgcgcggctc gaagcaccgc aggcccagct cgttgaagcg
     1321 gcgccacatg agctgacgcc gctcgtcgta cgccttcacc atgcgctgca cctccggctc
     1381 gccgacgcgg agcgcctcga gcgcggcgta ctgcgccgcg gtcggcacgc acatgacggt
     1441 gtactggtgg atcttcatca tctgctcgac gatctcggcc ggcgcgcacg cgtagccgac
     1501 acgccagccg gtcatcgcat aggacttgga gaatcccgcg aggtagaccg tgcgctgctt
     1561 catctcgggt atcgacgcga tcgagtggtg ctccaccccg tagacgaggc ggtcgtagat
     1621 ctcgtccgag tagacgagga gatcgtgctc ctgtgcgagc cgcgcgatgc cctcgacgtc
     1681 ttccctccgg agcacgccac cggtcgggtt gttcgggaac ccgagcagga tcgcgcgcgt
     1741 ccgcggcgtg atcgccttct ggaccgcggc cggggtgagg cggaactcct cggactcctc
     1801 ggtcggcacc ggcacgggca cgccaccggc gagcacgatg cccggcacgt acgccacgta
     1861 tgacggatcg gcgaggatga cctcgtcgcc gtcgttcagc gtcgcgcgga gcgccagatc
     1921 cacggcctcg gagacgccga ccgtcacgag gatctcgcgg tcggggtcgt agcgcacgcc
     1981 acgcagcttc gcggtgtgct cgctgatggc gacgcggagc ggcttgatgc cgtagttcga
     2041 ggtgtacttc gtccttccgt ccctgatcga gcggatcgcc gcctcgcgga acggctcggg
     2101 cgtgatgaag tcgggctccc ccacgccgag ggagatcgcg ccctcgacgc cggcgatcag
     2161 gtcgaagaag cggcggatac cgctcgccgg gatcttcgag acgcgcgtgt tcgtgagcgg
     2221 tcgcatcgtc ttcatcgtta tgccgcctcc tgtggtcgca tctcgtgatg cttcgtcgcg
     2281 cgctcgtatg ccgcggcgac gcggaacatc gtcgcctcct ggaacgagtt cgccatgacc
     2341 tgcagcccga ccggaagtcc ctcggagagc ccgcacggga tgctcatgcc gggcaggccg
     2401 gcgatgttgc ccggcagcgt gaacacgtcg ttcaggtaca tcgcgagcgg gtcgttcacc
     2461 ttctgtccga tcttgaaggc gaccgtcggc gaggtcggcg tcacgatggc gtcgcatcgc
     2521 tcgaaggccg tgtcgaactc cgccttgatg agcgtgcgca ccttctgcgc cttcacgtag
     2581 taggcgtcgt agtagccggc ggacagcgcg tacgtcccga ggatcatgcg gcgcttcact
     2641 tcggcgccga agccctcgcc acgcgtgcgg cggtaggtct cgacgagatc cttgccttcg
     2701 acgcggagcc cgaagcgcac gccgtcgtag cgggcgaggt tcgacgacgc ctccgcgggc
     2761 tggatgatgt agtagacggc gagcccgtgg tcggtgagcg agagggagac gtccaccagc
     2821 tcggcgccct ccttctcgta ccagcgcagc gcctcgcgga ccgcgcgctc gacacccgtt
     2881 tcgatcccct cgatgaagaa ctccttcggc accccgatgc ggagcccctt cacgcccttg
     2941 tccagctcgc gccgcaggtc ctcggtcgcg aggggcatgg tcgtcgcgtc gttcgggtcc
     3001 tgtccgaaga gcgcctggca cacgatcgcg cagtcctcca ccgtgttggt gaagggcccg
     3061 acctggtcga gcgaggacgc gaacgcgacg acgccgaagc gcgacacgcg gccgtacgtc
     3121 ggcttcatgc cgaccacgcc cgtgagcgcg cccggctgac ggatcgagcc gcccgtgtcg
     3181 gtaccgagcg cgaagagcgc ctgcccagcg gcgacggcga cggcgctgcc gcccgacgac
     3241 ccacccggga cgcgcgcggt gtcccagggg ttctgcaccg cgccgaacgc ggagttgtcg
     3301 ttcgacgagc ccatggcgaa ctcgtcgagg ttcgtcttgc cgatcgtgac cgcgccctcc
     3361 gcgtcgagcc gttcgacgac ggtcgccgag tacggcggca cgtatccctt gaggatcttc
     3421 gatgccgcgg tcgtctcgat gcccttcgtg ccgatgatgt ccttgcaggc ccacgggatc
     3481 ccggcgagga gcggcgcgtc gccgcgccgc aggcgctcgt cggccgcctt cgcctgcgcg
     3541 cgagcgtggt cgtcggcgac gagcagccac gcgttgtacc ggtcaccgtg tgcgcgcgcg
     3601 agcgcggcct cggtgacctc ggccgcgctg gcctcgcgcc ggcggagcag cgccgcggcc
     3661 tcggtgatgg aacgcggcag cgcgttcatc gctcctcgag gacctcctgg acgcggaaga
     3721 actcgccgtc gcgtccgccc ttcggggcgt tcgcgagggc ctgggcgttc gtgaggcccg
     3781 gccgcgcctc gtcgtcgcgc tgcacgtcct gcagcgggag caccgaggcg ctcggcagga
     3841 ccgcgctcgt gtcgacctcg ccgaggcgcg cgacggcgtc cagcacgacc gagagctgcc
     3901 ccgtgaaccg cagggtctcc tcgtcggaca ggcggatccg cgcgagatcg gcgacgtgaa
     3961 cgatggtctg gcgatcgatg ggcatgcgac gagtctaggt cgcggagcga cgcggttccg
     4021 tcgcggctac gtgccgcgcg actccagttt cagtgtccgt ccgtc
//
LOCUS       DASTZR010000483         2731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_333466, whole genome shotgun sequence.
ACCESSION   DASTZR010000483 DASTZR010000000
VERSION     DASTZR010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333466"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VFV20_10755"
     CDS             <1..957
                     /locus_tag="VFV20_10755"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU5288873.1"
                     /translation="DLVARAGVLLTNLPVREWSSYERLSARRPDLIMAVITGNPDGTT
                     AVDYTVNAAVGIPFLTGPEGHDGPVNHVLPAWDALTGYAVATAIVAAELHRVRTGEGQ
                     LITLSLMDVALSTVGHLGLIGEAQLAEPRGRYGNDVYGTFAHDFRTSDGRSVIVVALT
                     PRQWASLVAATGIADAVAALERRLGIDLRREGDRFRARREITALLAPWMAARPLTEIA
                     AILDRHEVLWGPYQTVKELVAADARCTTRNPMLAEVDQPGIGRYLRATSPISFGRSPR
                     VAPARSPRMGEDADAVLTSWLDLTPRDVAALQDEGVVGRVTE"
     gene            992..2134
                     /locus_tag="VFV20_10760"
     CDS             992..2134
                     /locus_tag="VFV20_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEU5288874.1"
                     /translation="MGPLAGVKVVELAGIGPAPFCAMLLADAGAEVLRIDRLEDTDLG
                     VRVDPRFNLLNRGRRSVAIDLKRADGVAAARRLVAQADVLIEGFRPGVMERLGLGPDD
                     CFQLNPRLVYGRVTGWGQDGPLSRSAGHDIDYIALAGVLGTIGPAEGEPVPPLNLVGD
                     YGGGALYLAFGILAALLEARTSRRGQVVDAAMVDGAASLATSAFGQLASGTLADRRGA
                     NVLDGGAPWYRAYETADGKFMAVGAIEPKFYAELLRVLGLAKEPLPAQHDRASWPQLR
                     DRFAAVFRTKTRDEWAALFTGTDACVAPVLSLAEARAHPHNAARGTFVSVDGVTQPAP
                     APRFSRTPSAISGGPALRGAHTDEALADWGFSADELAALRASGAIA"
     gene            2184..2681
                     /locus_tag="VFV20_10765"
     CDS             2184..2681
                     /locus_tag="VFV20_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288875.1"
                     /translation="MTHIPDGIRMHVLASANVFLGASNFVVIALPDRYTVGQTINPAE
                     VAGTHPARGAAWVLDGRCSHYLRDGQTRKSLELLVVTRRGETSLPRWYASSDARTIVV
                     AQHEARMLFDEIRVGFPRRRSMARVRAAWSCERTKRALEVELLGPHDADLAPIADAFA
                     LAECH"
BASE COUNT          348 a         1010 c          978 g          395 t
ORIGIN      
        1 gatctcgtcg cgcgcgccgg cgtgctgctt acgaacctgc ccgtccgcga atggtcttcg
       61 tacgagcgcc tctccgcgcg gcggcccgac ctcatcatgg cggtcatcac cggcaatccc
      121 gacggcacga ccgcggtcga ctacacggtg aacgccgcgg tcgggatccc gttcctgacc
      181 ggaccggagg gccacgacgg gcccgtgaac cacgtgcttc ccgcgtggga cgcgctcacc
      241 gggtacgcgg tggcgaccgc gatcgtcgcg gccgagctgc accgcgtgcg caccggcgag
      301 ggacagctca tcacgcttag cctgatggac gtcgcgctct cgacggtcgg gcatctcggg
      361 ctcatcggcg aggcgcagct cgcggagccg cgcgggcgct acggcaacga cgtgtacggc
      421 acgttcgcgc atgacttccg cacatccgac ggccgctccg tgatcgtcgt cgcgctcacg
      481 ccacgccagt gggcgagcct cgtcgccgcg acggggatcg cggacgcggt cgccgcgctc
      541 gagcggcgtc tcggcatcga tctccgcagg gaaggcgacc gcttccgcgc tcgccgcgag
      601 atcaccgcgc tcctcgcgcc gtggatggcc gcgcggccgc tcaccgagat cgcggcgatc
      661 ctcgatcggc acgaggtgct gtggggtcca tatcagacgg tgaaggagct cgtcgccgcc
      721 gacgcgcgct gcacgacgcg caacccgatg ctcgccgagg tcgaccagcc cggcatcggc
      781 cgctacctgc gcgcgacgtc gccgatctcg ttcggtcgct cgccgcgcgt cgcgccggcg
      841 cggtcgccgc gcatggggga ggacgcggac gcggtgctca cctcgtggct cgacctcacg
      901 ccgcgcgacg tcgcggcgct ccaggacgag ggcgtcgtcg gacgcgtgac ggagtagcgc
      961 atgcttcggc tcgtggatgt gacggagtag ggtgggtccg ctcgcgggcg tgaaggtcgt
     1021 cgagctcgcc ggtatcggac cggcgccgtt ctgcgcgatg ctgctcgccg acgccggcgc
     1081 ggaggtgctg cgcatcgatc gcctcgagga caccgatctc ggcgtgcgcg tcgatccgcg
     1141 cttcaacctg ctcaaccgcg ggcggcgctc cgtcgcgatc gatctcaagc gggctgacgg
     1201 cgtcgccgcc gcgaggcggc tcgtcgcgca ggccgatgtg ctcatcgagg gattccgccc
     1261 cggcgtgatg gagcggctcg ggctcggccc cgacgactgc ttccagctca acccgcgcct
     1321 cgtgtacgga cgcgtgaccg gctggggaca ggacggccca ctcagccggt ccgcgggcca
     1381 cgacatcgac tacatcgcgc tcgccggcgt gctcgggacg atcggtccgg cagaaggcga
     1441 gcctgtgccg ccgctcaatc tcgtcggcga ttacggcggc ggcgcgctct acctcgcgtt
     1501 cggcatcctc gccgcgctgc tcgaggcgcg cacgtcccgg cgagggcagg tcgtcgacgc
     1561 ggcgatggtc gacggcgcgg cctcgctcgc gacgagcgca ttcggccagc tcgcgtcggg
     1621 cacgctcgcc gaccggcgcg gagcgaacgt gctcgacggc ggcgcgccct ggtaccgcgc
     1681 gtacgagacc gccgacggga agttcatggc ggtcggcgcg atcgagccga agttctacgc
     1741 cgagctgctg cgcgtcctcg gtctcgcgaa ggagccgctc ccggcgcagc acgaccgcgc
     1801 gtcgtggcca cagctccgcg accgcttcgc cgccgtcttc cgcacgaaga cgcgcgacga
     1861 gtgggcggcg cttttcaccg gcaccgacgc gtgcgtcgcg ccggtgctca gcctggcgga
     1921 ggcgcgcgca cacccgcaca acgcggcgcg cgggacgttc gtgagcgtgg acggcgtgac
     1981 gcagccggcg cccgcgccgc gcttcagccg cacgccgtcc gcgatcagcg gcggcccggc
     2041 cctccgcggc gcgcacaccg acgaggcgct cgcggactgg ggcttctcgg ccgacgagct
     2101 cgccgcactg cgtgcgagcg gtgcgatcgc gtgacgccgg cggggccgtg gctccaccgt
     2161 tcggcgggtc atagcctgta gccgtgacgc acatccccga cggcatccgt atgcacgtgc
     2221 tcgcgtcggc gaacgtgttc ctcggcgcga gcaacttcgt cgtcatcgcg ctgcccgatc
     2281 gctacacggt cgggcagacc atcaatcccg ctgaggtcgc ggggacgcat cccgcccgcg
     2341 gtgcggcgtg ggtgctcgac ggccgctgct cgcactacct tcgcgacggc cagacgcgta
     2401 agtcgctcga gctgctcgtc gtcacgcggc gcggcgagac gtcactgccg cgctggtacg
     2461 cctcctccga cgcgcgcacg atcgtcgtcg cgcagcacga ggcgcgaatg ctcttcgacg
     2521 agatccgcgt cggcttcccg cgccgccgct cgatggcccg cgtccgcgcc gcgtggtcgt
     2581 gcgagcgcac gaagcgcgcg ctcgaggtgg agttgctcgg tccacacgac gccgacctcg
     2641 caccgatcgc cgacgctttc gcgctcgcgg agtgccacta gaagcacgtt ggcggtgatc
     2701 ggcggtccat actccgtcgg atgctggtcc a
//
LOCUS       DASTZR010000484         3710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_386177, whole genome shotgun sequence.
ACCESSION   DASTZR010000484 DASTZR010000000
VERSION     DASTZR010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386177"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2567)
                     /locus_tag="VFV20_10770"
     CDS             complement(<1..2567)
                     /locus_tag="VFV20_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5288876.1"
                     /translation="MATRVLIVDDHLAIREGIRSLLAPERDFVVVAEAVDGNDGIAKA
                     LSLRPDLIVLDNSMPGKTGLEVARELRPQLPDTAIVFLTLDPGIRDLALAVGAIAHIS
                     KDTPPQQMLKVLRNAAEQQERRRNAPPTLSEKERALATALLEAKLLTEQQVDTLIAQR
                     HPRELLSAALLRAKLVPDVQLAQVLSRVSGRPLMTLAPRPEGSVPSGPRTTGRAARRL
                     IDPIDPTVARQIPRRFSEERHTILVTFGRSEAILALADPFDDQTRREVADMLAGLHVT
                     VVVATLGDIDGAIARAFGAPTANLVTFPAAAHRRQSRRRPLMLVAAIAAMFLLFATGL
                     GILLAPATSGLQARAQLTIFQGTVEYRRAGGAYTTVASGQLVRQGDSVRTGANAHAAL
                     TFFDNSVIVFDPATEIEIVSLRALSGGDIDATLRQTSGETWHVVSHQLTTQGRYAVLT
                     PTSQTSVTGTAFQVRVEAATGATTVTTTDGVVRTSGIDEAASTTMPVTPGSGTVVSAK
                     GARPQAPIPMPQPMVTFTLDTSRDAVVVAPNGEASGVRSGQVIRYIPGSTVAVKGTQV
                     IVSVPGDTDRYAAIVQPADATAATVNVEAQLRTTSGAVAGQITDKATVAGGVGKSGVG
                     VTPQGLVSLSGTVVDQFPAPIVVAAPPAATFDPLAFVRTGASPGNPGPAGAPGSTGPA
                     GPPGPQGPPGPTGAQGPSGVAGATGPSGLQGATGVQGAAGPSGPAGVPGAEGPTGPAG
                     ATGAQGPTGASGAPGTAGVPGATGPAGATGQAGGTGAQGATGAQGSTGPQGPTGNTGA
                     QGATGPQGVQGPTGPQGTQGSTGAQGPQGATGAQGATGAQGATGDTGAQGATGDTGA"
     gene            2790..3239
                     /locus_tag="VFV20_10775"
     CDS             2790..3239
                     /locus_tag="VFV20_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288877.1"
                     /translation="MTAAKVLVVVAHPVIASGIETLLRLEADYDVRRVASIPEAAKQQ
                     EWHPDVALIDGTLLTGYVDATIGAPAFVLSGNERDGRQLARRLDDGRGWLRKDATGKE
                     LAQAIRSAMRGEEAGATGLGTLGVVTIALLSLVVLALVAYLLWLAVY"
     gene            3260..>3710
                     /locus_tag="VFV20_10780"
     CDS             3260..>3710
                     /locus_tag="VFV20_10780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5288878.1"
                     /translation="MTRVMLVDDHELVRQGIAAMLNGAGDVSVVGVARTGREALEVAR
                     RELPDVVLMDVKMPDMDGLEATRKMKEERPRTAVVMLTMHDNPAYLRDAVRAGAAGYL
                     LKDVSKDELIDAIRQVATGGAFIESQMLKGMLSEMKPSGGVGPSPAAK"
BASE COUNT          524 a         1288 c         1346 g          552 t
ORIGIN      
        1 tgcgcaccgg tgtcgcccgt cgcgccttgc gcacctgtgt cgcccgtcgc gccttgcgcg
       61 ccggtcgcac cttgcgcacc tgtcgcgcct tgcggacctt gcgcgccggt cgatccttga
      121 gtgccctgcg gtcccgtcgg accctggaca ccttgcggac cggtcgcacc ttgcgcaccg
      181 gtgttgcccg tcggaccttg cggtccggtg ctgccctgcg cgccggtcgc accctgcgcg
      241 cccgtaccgc ccgcctgtcc cgtcgcgccg gcagggcccg tggcacctgg gacacccgcg
      301 gtgccgggcg cgcctgacgc gccagtggga ccctgcgcac cagtcgcgcc agcgggcccg
      361 gtcgggccct cagcaccggg aacgcccgca ggaccagacg gccccgcggc gccttggacg
      421 ccggtcgcgc cttgaagtcc gctcggaccg gtggcgcccg ccacgcccga aggaccctgt
      481 gcacccgtcg gcccgggagg accctgcggg ccgggaggac ccgccggacc ggtcgaaccg
      541 ggagcgccgg ccggaccggg attgcccgga ctcgctccgg tgcgcacgaa cgcaagggga
      601 tcgaaggtcg cagcgggcgg tgccgcgacg acgatcggag ctgggaactg atcgaccacg
      661 gtgccggaca gcgagacgag cccctgcggc gtcacgccga caccggactt cccgacgcca
      721 ccggcgaccg tcgccttgtc cgtgatctgt cctgcgaccg cgccgctcgt cgtgcgcagc
      781 tgcgcctcga cgttcaccgt cgcggccgtc gcgtcggcag gctgcacgat cgccgcgtat
      841 cggtcggtgt cccccggcac ggacacgatg acctgcgtgc ccttcaccgc cacggtcgac
      901 cccgggatgt agcggatgac ctggccgctg cggacgcctg acgcctcgcc gttgggagcg
      961 accaccacag cgtcgcgcga cgtgtcgagc gtgaaggtca ccatcggctg cggcatgggg
     1021 atcggcgcct gcggccgtgc gcccttcgca ctcacgacgg tgccgctgcc cggcgtgacc
     1081 ggcatcgtcg tggaggcggc ctcgtcgatg ccactcgtgc gcacgacgcc atcggtcgtc
     1141 gtcaccgtcg tcgcgcccgt cgcggcttcg acgcgcacct ggaacgcggt gccggtgacc
     1201 gacgtctgcg acgtcggcgt cagcaccgcg tagcgcccct gcgtggtgag ctgatgcgag
     1261 accacatgcc acgtctcacc ggaggtctgc cgcagcgtcg cgtcgatgtc gccgccgctg
     1321 agcgcgcgga gggacacgat ctcgatctcg gtcgcgggat cgaagacgat gaccgagttg
     1381 tcgaagaacg tcagcgcggc gtgcgcgttc gctccggtgc gaacgctgtc gccctgccgg
     1441 acgagctgac ccgacgcgac agtcgtgtac gcgccgcctg cgcgacggta ctcgaccgtg
     1501 ccctggaaga tcgtgagctg cgcgcgcgcc tggagaccgc tcgtcgccgg cgcgagcagg
     1561 atgccgagcc ccgtcgcgaa gaggaggaac atcgccgcga tcgcggcgac gagcatgagc
     1621 ggccggcgac gcgactgccg acggtgcgcg gccgcgggga acgtgactag gttcgctgtg
     1681 ggcgcgccga acgcgcgcgc gatcgcgcca tcgatgtcac cgagcgtcgc gaccacgacg
     1741 gtgacgtgca gacccgcgag catgtccgcc acctcgcggc gcgtctggtc gtcgaacgga
     1801 tccgcgagcg cgagtatcgc ctcgctgcgg ccgaaggtga cgaggatcgt gtgccgctct
     1861 tcgctgaagc gacgggggat ctgccgcgcg acggtcggat cgatcgggtc gatcagccgt
     1921 cgcgcggcac gccccgtcgt ccgagggccg ctgggaacgg atccctcggg acgcggcgcg
     1981 agcgtcatga ggggacgccc gctgacgcgg gacaggacct gggcgagctg gacgtcgggg
     2041 acgagcttcg cgcgcagcag cgcggccgag agaagctcgc gcggatggcg ttgcgcgatg
     2101 agcgtgtcga cctgctgctc ggtgaggagc ttcgcctcga ggagcgccgt ggcgagtgcg
     2161 cgctccttct cgctcagcgt cggcggcgcg ttgcgtcgac gctcctgctg ctcggcggcg
     2221 ttgcgcagca ccttcagcat ctgttgtggc ggcgtgtcct tgctgatgtg cgcgatcgcg
     2281 ccgaccgcga gggcgaggtc gcggatgccg ggatcgagcg tgaggaagac gatggccgtg
     2341 tccgggagct gcggccgcag ctcgcgcgcg acctcgaggc cggtctttcc gggcatcgag
     2401 ttgtcgagga cgatgaggtc gggtcgcagg gagagcgcct tggcgatgcc gtcgttgccg
     2461 tccaccgctt cagcgacgac cacgaagtcg cgctcaggcg cgaggagaga acggatccct
     2521 tctcggatcg cgaggtggtc gtcaacgatc agtacgcgag tcgccacgcc cttcgcctat
     2581 ccctctcggt cacgctccct cacggcgatc cgtggggtgt ccggcggttc ccagcggccg
     2641 ggcacgtgga taggctcgtt tttcgggtgg tcccgggcat cggacgccgg gcggagaaag
     2701 gtaggacggc cgtgtcgcaa aatgcgacaa tgtgggcatt gaagcgacag gtcgctcgcc
     2761 aggcgggcgc tcctattgaa taggccgctg tgacagcagc gaaggtcctc gtcgtcgtcg
     2821 cccacccggt gatcgcgtca gggatcgaga cgctgctgcg actcgaggcg gactatgacg
     2881 tccgccgcgt cgcgagcatc cccgaggcgg cgaagcagca ggagtggcat cccgacgtgg
     2941 cgctcatcga cggcacgctg ctgaccggct acgtcgacgc gaccatcggc gcgcccgcat
     3001 tcgtgctctc gggcaacgag cgcgatggac gtcagctcgc gcgcaggctc gacgacggtc
     3061 gtggatggct gcgcaaggac gcgaccggca aggagctcgc acaggcgata cgcagcgcga
     3121 tgcgtggcga ggaggccggc gccaccggtc tcggcacgct cggcgtcgtc acgatcgcgc
     3181 tcctctcgct cgtcgttctc gcgctcgtcg cgtatctcct gtggctcgcg gtgtactagg
     3241 tcgtcctaga atccgctcga tgactcgagt gatgctcgtt gatgaccacg agctcgtccg
     3301 ccagggcatc gcggcgatgc tcaatggcgc gggcgatgtg agcgtcgtcg gcgtcgcgcg
     3361 gaccgggcgc gaggcgctcg aggtcgcgcg ccgagagctc ccggacgtcg tcctcatgga
     3421 cgtgaagatg cccgacatgg acgggctcga agccacgcgc aagatgaagg aagagcgccc
     3481 gcgcaccgcg gtcgtcatgc tgacaatgca cgacaacccc gcataccttc gcgacgcggt
     3541 ccgcgccggc gccgcgggct atctccttaa ggacgtctcg aaggacgagc tcatcgacgc
     3601 catccgccag gtcgcgaccg gcggagcatt catcgagtcg cagatgctca aggggatgct
     3661 cagcgagatg aagccgagcg gcggtgtcgg gccgagtccc gccgcgaaga
//
LOCUS       DASTZR010000485         2768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_570820, whole genome shotgun sequence.
ACCESSION   DASTZR010000485 DASTZR010000000
VERSION     DASTZR010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570820"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VFV20_10785"
     CDS             complement(<1..302)
                     /locus_tag="VFV20_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEU5288879.1"
                     /translation="MAVIDANVLVLNLDYQPLNVCNVRRAVVLLAKEKATVIEQNGHI
                     VTSERLRLPSPSVIRLAYHVKRPRPVVKLTRKEVLQRDDHLCQYCGKRGNDLTLDHV"
     gene            342..1256
                     /gene="pdxS"
                     /locus_tag="VFV20_10790"
     CDS             342..1256
                     /gene="pdxS"
                     /locus_tag="VFV20_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEU5288880.1"
                     /translation="MTTSQNGSNGQGKTGTWTLKTGLAQMLKGGVIMDVVTAEHAKIA
                     EEAGAVAVMALERVPADIRATGGVARMSDPEKIIEIMSAVTIPVMAKARIGHFVEARV
                     LEALGVDYIDESEVLTPADEQFHIEKHDFKVPFVCGARDLGEALRRINEGAAMIRSKG
                     EAGTGNVVEAVRHLRAISSQLRALADMDEGALAATAKEYRVPVDLVKRVAKEQKLPVV
                     LFCAGGIATPADAALMMQLGAEGNFVGSGIFKSSDPAKRAKAIVEATTHYTDAKLIAE
                     VSRGLGEPMRGLALETIPPAERLAVRGW"
     gene            1285..1842
                     /gene="pdxT"
                     /locus_tag="VFV20_10795"
     CDS             1285..1842
                     /gene="pdxT"
                     /locus_tag="VFV20_10795"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012984254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEU5288881.1"
                     /translation="MGLQGDFAEHLRILREMGIDAVDVRRAEQLDDVEALIIPGGEST
                     TIGKLAKQYDILPKLQERVAAGMPVWGTCAGAIFLAKDVPGHPHPIAGLMDISVERNA
                     FGRQLDSFEADLDVKGIAGQPYHAVFIRAPKITPTGNDVETLATLDDGTVVAARQGNL
                     LATSFHPELTRDDRFHRYFLSLGKR"
     gene            1842..>2768
                     /locus_tag="VFV20_10800"
     CDS             1842..>2768
                     /locus_tag="VFV20_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288882.1"
                     /translation="MPARAATPFDAPRISSLLERSLGTADAVLASALPVPFAAGTVTA
                     FGRLTLGLLGVTGGRMRVFLEEYRGQLRSAALVYDGRRPEWVIVVLGALHEPGGADSA
                     FRLLSGISAAAARAGVHRVYAAVPDETRARETFFQAGFYSYTRETWYVATRAQLEPAQ
                     CEGLREARASDAHDLFRFYVATTPHAVQRAEQLSVPDFDVSRRGGAYDPPHLIGGNPL
                     AMHREAAFVVGDERSTRAFGFAFAGRDRHPHVCKVRTRDGDVDLARDLVRASVRALPA
                     GRPITAPVRSYEEHVGRALLAEGFRDMATAMLF"
BASE COUNT          420 a          957 c          980 g          411 t
ORIGIN      
        1 acgtggtcga gcgtcaggtc gttgccgcgc ttgccgcagt actggcagag gtggtcgtcg
       61 cgctggagga cttccttgcg cgtcagcttc acgaccggcc gcggccgctt cacgtggtag
      121 gcgaggcgga tgaccgaggg agacggcaag cgcagccgct cactggtgac gatgtggccg
      181 ttctgctcga tgacggtggc cttttccttg gcgagcagga ccacagcgcg tcgtacgtta
      241 cacacgttca gcggctgata gtcgaggttg agcacaagga cgttcgcgtc gatgaccgcc
      301 atatcgagat tctagggtcc gcgcggtacg attgcgtgta aatgaccacc tctcagaacg
      361 gttccaacgg acaggggaag accggcacgt ggaccctcaa gacgggtctc gcgcagatgc
      421 tcaagggcgg cgtgatcatg gacgtcgtca ccgccgagca cgcgaagatc gcggaggaag
      481 cgggtgcggt cgcggtgatg gcgctcgagc gcgtgcccgc ggacatccgc gcgaccggcg
      541 gcgtcgctcg catgagcgac ccggagaaga tcatcgagat catgtcggcc gtcaccatcc
      601 cggtgatggc caaggcacgc atcgggcact tcgtcgaggc gcgcgtgctc gaggcgctcg
      661 gggtcgatta catcgacgag agcgaagtgc tcacgcccgc cgacgagcag ttccacatcg
      721 agaagcacga cttcaaggtc ccgttcgtct gcggcgcgcg cgatctcggt gaggcgctcc
      781 gccgcatcaa cgaaggcgcc gcgatgatcc gcagcaaggg cgaggccggg accggcaacg
      841 tcgtcgaggc cgtccgccac ctgcgcgcga tctcgtcgca gctcagggcg ctcgccgaca
      901 tggacgaagg cgcgctcgca gcgaccgcga aggagtaccg cgtccccgtc gacctcgtga
      961 agcgcgtcgc caaagagcag aagctcccgg tcgtgctgtt ctgcgccggg ggtatcgcga
     1021 cgcccgcgga cgccgcgctg atgatgcagc ttggtgccga gggcaatttc gtgggttccg
     1081 ggatcttcaa gtcgagcgat cccgccaagc gcgcgaaggc catcgtcgaa gcgacgacgc
     1141 actacaccga cgccaagctc atcgccgagg tgtcgcgcgg actcggcgag ccgatgcgcg
     1201 gtctcgcgct cgagacgatc ccgcccgccg agcgacttgc cgtccgcggc tggtagcgac
     1261 ccaaagaccg cacgcgtcgg cgtcttggga ttacagggcg acttcgccga gcacctgcgc
     1321 atcctgcgcg agatgggtat cgacgccgtc gacgtgcgca gggcggagca gctggacgat
     1381 gtcgaggcgc tcatcatccc cggcggtgag agcaccacga tcggaaagct cgcgaagcag
     1441 tacgacatcc tcccgaagct gcaggagcgc gtcgccgcgg gcatgccggt gtgggggacc
     1501 tgcgcgggcg cgatcttcct cgcgaaggac gtgcccgggc atccccaccc gatcgcgggg
     1561 ctgatggaca tcagcgtcga gcggaacgcg ttcggccgcc agctcgactc gttcgaggcg
     1621 gacctcgacg tgaagggcat cgccggtcag ccgtaccacg cggtcttcat ccgcgcgccg
     1681 aagatcaccc cgaccggcaa cgacgtcgag acgctcgcga ccctggatga cgggaccgtg
     1741 gtcgcggcac gacaggggaa tctgctcgcg acgtcgttcc acccggagct gacgcgcgac
     1801 gatcgcttcc accgctactt cctgtcgctc gggaagcgct gatgcccgcg cgcgcggcca
     1861 cgccgttcga cgcgccgcgc atctcgtcgc tgctggagcg ctcgctcggc acggcggacg
     1921 cggtgctcgc ctcggcgtta ccggtgccgt tcgcggccgg cacggtgacc gcgttcggcc
     1981 gcctgacgct cggcctcctc ggcgtcaccg gcgggcggat gcgcgtcttc ctcgaggagt
     2041 accgcgggca gctgcgcagc gccgcgctcg tgtacgacgg acgccggccg gagtgggtga
     2101 tcgtcgtcct cggcgcgctc cacgagccgg gcggcgcgga cagcgcgttc cgcctgctgt
     2161 cgggcatcag cgccgccgcg gcgcgggccg gggtccaccg cgtctacgcc gcggtccccg
     2221 acgagacccg cgcgcgggag acgttcttcc aggccggctt ctactcgtac acgcgcgaga
     2281 cctggtacgt ggccacgcgc gcgcagctcg agccggcgca gtgcgagggc ctgcgcgagg
     2341 cgcgcgcgag cgacgcgcac gatctgttcc gcttctacgt cgcgaccacc ccgcacgccg
     2401 tccagcgcgc cgagcagctc tcggtccccg atttcgacgt ctcccggcgc ggcggcgcgt
     2461 acgacccgcc gcatctcatc ggcgggaacc cgctggcgat gcaccgcgag gccgccttcg
     2521 tcgtgggcga cgagcgctcg acgcgcgcgt tcgggttcgc gttcgccggc cgcgatcgcc
     2581 atccgcatgt ctgcaaggtg cgcacgcgag atggcgacgt ggacctcgct cgcgacctgg
     2641 tccgcgcgag cgtgcgcgcg ctgcccgcgg gacggcccat caccgcaccg gtgcgctcgt
     2701 atgaggagca cgtcgggcgc gcgctgctcg ccgagggctt ccgggacatg gcgacggcga
     2761 tgctcttc
//
LOCUS       DASTZR010000486         3808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1223287, whole genome shotgun sequence.
ACCESSION   DASTZR010000486 DASTZR010000000
VERSION     DASTZR010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223287"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1286
                     /locus_tag="VFV20_10805"
     CDS             <1..1286
                     /locus_tag="VFV20_10805"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEU5288883.1"
                     /translation="TGLEWQYEQYLRGTYGWREIERDAAQREIKTLAYAPPTQGNTVV
                     LTLDTRLQNIIDAELRKGVEEDKFTQAVGIAMNPQNGEILAMVSIPGYDNNWFVQGIT
                     PEQMKQLNSDDRRPLVNKAVGDIYPPGSTFKMVTGLSALTAGTATRNTVVNVNSMVMT
                     VSGFNFYDWRAHGSLDFLNAFAHSSDIYFYTLGGGSPMGPGIAGAGPVNIAKYGRMLG
                     FGARTGIDLPGEARGIMPDPQWKLDTFDEEWTIGNTYHESIGQGYVAVTPIQLLNAYA
                     SVANGGTVYTPHLLKEVRDPEGGTIYTQAPKVTRKLEMKAEDLRLIRESARRVVTVGH
                     AYMPNAKLPIAGKTGTAEFGAATGKDSAGRNRLGFHNWFVSFLPQQDNTDPTAQIAMV
                     LFTYDSSRSLCDVCFNPAVAMTQKILETYVLGDKK"
     gene            1283..2446
                     /gene="rodA"
                     /locus_tag="VFV20_10810"
     CDS             1283..2446
                     /gene="rodA"
                     /locus_tag="VFV20_10810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEU5288884.1"
                     /translation="MSVKAMALPRAASPFHDRPHVRRFDQVDPFLLIATLLLIAYGLV
                     MTYSVTAESRTFTGDPMQFVYRGAIWAAIGLGVMVAVAWFDYAWLGTLAPLLYLLTLG
                     LLAVVLAIGHESLGAQRSVNFGGLTFQFSEVAKVLAIIVLAKYFADRSDKVGSPLTIV
                     GGLVLLGPPVLLVYKQPDLGTSLVFIAIFFGLAFMAGARVWQLVSLVAMGGAAFPLMW
                     SLLKPYQQGRLTAFLNPYADPQGAGWNIIQSLIAVGSGAATGKGLTAGTQSPLGYLPI
                     AESDFVFAGLAEDLGFMGAVILFALYIVLLVAALRIAFRSRDAFGTFLGGGIVAMLLF
                     QIMVNVGMAISLMPVTGIPLPFISHGGSSLVSICFALGVLQSVSMRRAAPELG"
     gene            complement(2478..2996)
                     /locus_tag="VFV20_10815"
     CDS             complement(2478..2996)
                     /locus_tag="VFV20_10815"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEU5288885.1"
                     /translation="MYRLVRVDKRIPNGSIWQARRAYLLPAIDGWTRVFGPTGTRWSN
                     PLGGWTTQHGGMSLFHPAQPFTISCYGEPGQERFYVDIAHRVRIATDSIEFVDLFLDV
                     MIDPSGNVTEKDEHQLIVLPEAVRAFTRRARDEVRSLIGRRHELFDRASRYYALPDGA
                     RELPPAIDPLEL"
     gene            3185..3256
                     /locus_tag="VFV20_10820"
     tRNA            3185..3256
                     /locus_tag="VFV20_10820"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3217..3219,aa:Ala,seq:ggc)
     gene            3367..>3808
                     /locus_tag="VFV20_10825"
     CDS             3367..>3808
                     /locus_tag="VFV20_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019585347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEU5288886.1"
                     /translation="MTIEKQRTERYDPAAIEPKWLAEWERTGLHQTPDAVPGKENFYY
                     LTMFPYPSGVLHVGHWYAFAAPDAVARHLRMRGYNVLFPMGFDAFGLPAENAAIKRKL
                     HPAIWTEKNMAHMREQFHRMGAAIDWRREVVTCYPDYYRWNQWMF"
BASE COUNT          615 a         1277 c         1296 g          620 t
ORIGIN      
        1 gcaccgggct cgagtggcag tacgagcagt acctccgcgg cacctatggc tggcgcgaga
       61 tcgagcgcga cgccgcccag cgcgagatca agacgctcgc gtacgcgccg ccgacgcagg
      121 gcaacacggt ggtgctcacg ctcgacacgc ggctgcagaa catcatcgac gccgagctgc
      181 ggaagggtgt cgaggaggac aagttcaccc aggccgtcgg gatcgcgatg aatccgcaga
      241 acggcgagat cctcgcgatg gtctcgatcc ccggctacga caacaactgg ttcgtgcagg
      301 ggatcacgcc cgagcagatg aagcagctca acagcgacga ccggcgaccg ctcgtgaaca
      361 aggcggtggg ggacatctac ccgcccggtt cgacgttcaa gatggtgacc ggcctctcgg
      421 ccctgaccgc cgggaccgcg acgcgcaaca ccgtcgtgaa cgtgaactcc atggtcatga
      481 cggtgagcgg cttcaacttc tacgactggc gcgcgcacgg ctcgctcgac ttcctgaacg
      541 ccttcgccca ctcgtcggac atctacttct acacgctggg cggcggctcg ccgatggggc
      601 ccggcatcgc cggcgccggc ccggtcaaca tcgcgaagta cgggcggatg ctcggattcg
      661 gcgcgcgcac gggcatcgac ctccccggtg aggcccgcgg catcatgccc gacccgcagt
      721 ggaagctcga caccttcgac gaggagtgga ccatcggcaa cacctaccac gagtcgatcg
      781 ggcagggtta cgtcgcggtg acgccgatcc agctcctgaa cgcgtacgcg tcggtggcca
      841 acggcgggac ggtctacacg ccgcacctgc tgaaggaggt gcgcgacccc gagggcggga
      901 cgatctacac gcaggcgccg aaggtcacgc gcaagctcga gatgaaggcc gaggacctgc
      961 gactcatccg cgagtccgcg cggcgcgtcg tgaccgtcgg ccacgcctac atgccgaacg
     1021 cgaagctgcc gatcgcgggc aagaccggca ccgccgagtt cggcgcggcg accgggaagg
     1081 acagcgcggg ccgcaaccgt ctcgggttcc acaactggtt cgtctcgttc ctgccgcagc
     1141 aggacaacac cgacccgacc gcgcagatcg cgatggtgct cttcacctac gactcgagcc
     1201 gctcgctctg cgacgtgtgc ttcaaccccg cggtcgcgat gacccagaag atcctcgaga
     1261 cctacgtgct gggcgacaag aagtgagcgt caaggcgatg gcgctgccgc gtgcggcgtc
     1321 gccgttccac gatcggccgc acgtgcggcg cttcgaccag gtggatccgt tccttctcat
     1381 cgcgacgctg ctcctcatcg cctacgggct cgtgatgacg tactcggtga ccgcggagtc
     1441 gcgcaccttc accggcgatc cgatgcagtt cgtgtatcgc ggcgcgatct gggcggccat
     1501 cgggctcggc gtgatggtgg cggtcgcgtg gttcgactac gcgtggctcg gcacgctggc
     1561 gccgctcctg tatctgctca cgctggggct gctcgccgtc gtcctcgcga tcgggcacga
     1621 gtcgctcggc gcgcagcgtt cggtgaactt cggcgggctc accttccagt tcagcgaggt
     1681 ggcgaaggtc ctcgcgatca tcgtcctcgc gaagtacttc gccgaccgca gtgacaaggt
     1741 cggttcaccg ctgaccatcg tcggcggcct cgtactgctc gggccaccgg tgctcctcgt
     1801 ctacaagcag cccgatctcg ggacgagcct cgtcttcatc gccatcttct tcgggctcgc
     1861 gttcatggcc ggcgcccggg tctggcagct cgttagcctg gtcgcgatgg gcggcgcggc
     1921 gttcccgctc atgtggtcgc tcctgaagcc gtaccagcag gggcggctca ccgcgttcct
     1981 gaatccgtac gccgacccgc agggagctgg ctggaacatc atccagtcgc tcatcgccgt
     2041 cgggagcggt gcggcgaccg gcaagggcct caccgcgggc acgcagtcgc cgctcgggta
     2101 tctccccatc gccgaatccg atttcgtctt cgcgggcctc gccgaggacc tcggcttcat
     2161 gggcgccgtg atcctcttcg cgctctacat cgtgctgctc gtcgcggccc ttcggatcgc
     2221 gttccgctcg cgtgacgcgt tcgggacgtt cctcggcggc ggcatcgtcg cgatgctcct
     2281 gttccagatc atggtgaacg tcggcatggc gatctcgctc atgccggtca ccggcatccc
     2341 gctgcccttc atctcacacg gcggcagctc gctcgtgtcg atctgtttcg cgctcggcgt
     2401 gctgcagtcg gtgtcgatgc gacgggccgc gccggagctc gggtaaacgc cgagcgacaa
     2461 cgagagggcg aggagaacta cagctcgagc gggtcgatcg ccggcggcag ctcccgtgcg
     2521 ccgtcgggga gcgcgtaata gcgcgaggcg cggtcgaaga gctcgtgccg tcggccgatg
     2581 agggagcgga cctcgtcgcg cgcgcggcgc gtgaaggcgc gcaccgcctc cgggagcacg
     2641 atgagctggt gctcgtcctt ctccgtcacg ttgccactcg gatcgatcat cacgtcgagg
     2701 aacaggtcga cgaattcgat gctgtcggta gcgatgcgca cgcggtgcgc gatgtccacg
     2761 tagaagcgtt cctggcccgg ctcgccgtag caggagatcg tgaacggctg cgccggatgg
     2821 aacagcgaca tgccgccgtg ctgggtcgtc cagccgccga gcgggttcga ccagcgggtg
     2881 cccgtcggac cgaacacgcg cgtccagccg tcgatcgcgg gcagcagata tgcgcgccgc
     2941 gcctgccaga tggagccgtt cggtatgcgc ttgtcgacgc gaacgagccg gtacatccgc
     3001 ctatactttc tcagcaaagg ccgcgaacgg gagcagtacg cggatccgtg cgatcaagag
     3061 agccggtggg tggtgcgagc cggcatcgac ggacgcggaa ctcgcccggg agccgcgagg
     3121 gtgaagcgat gtagccctcg ccggagtcac gcccgttagc agcgtgctcg agcggagagc
     3181 gatcggggct gtggcgcagt gggagcgcat cacactggca gtgtgggggt cgcgggttcg
     3241 aatcccgcca gctccaccga taggttctca atcggggtgg taccgcggaa gtcgccttcc
     3301 gtcccctggc cgatcgttcg gcgtggggcg gagggctttt tgtttgggcg aaaggaggcg
     3361 ggcgcggtga ccatcgagaa gcagcgcacc gagcgctacg accccgcggc catcgagccg
     3421 aagtggctgg ccgagtggga gcgcacgggc ctccaccaga ccccggacgc ggtcccagga
     3481 aaggagaact tctactacct caccatgttc ccgtacccct cgggcgtgtt gcacgtgggg
     3541 cactggtacg cgttcgccgc gcccgacgcg gtcgcccgcc acctgcgcat gcgcggatac
     3601 aacgtgctgt tcccgatggg cttcgacgcg ttcgggctgc ccgcggagaa cgccgccatc
     3661 aagaggaagc tgcatccggc gatctggacc gagaagaaca tggcccacat gcgggaacag
     3721 ttccaccgca tgggcgccgc gatcgactgg cggcgcgagg tcgtcacgtg ctacccggac
     3781 tactaccgct ggaaccagtg gatgttcc
//
LOCUS       DASTZR010000487         3023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_858543, whole genome shotgun sequence.
ACCESSION   DASTZR010000487 DASTZR010000000
VERSION     DASTZR010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858543"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VFV20_10830"
     CDS             complement(<1..313)
                     /locus_tag="VFV20_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018305308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU5288887.1"
                     /translation="MRITRTFVTLAVAAGLVAGACGGAGSTASPTPAPATSAPAAATS
                     TPAPTWTPTKDVELVVQAAAGGGSDIFARKIADVLTKEKLSAKPINVVNKPGGSGAVA
                     YA"
     gene            complement(310..1011)
                     /locus_tag="VFV20_10835"
     CDS             complement(310..1011)
                     /locus_tag="VFV20_10835"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU5288888.1"
                     /translation="MQSLSVARPARTTAVSRVHGAILRRIENGELGPGTRLREEEVAR
                     RHGVSRTPVREAFRWLQAEGLVTVLPHVGAQVSDITLAEIDELFEIRGALEVLAVQRA
                     ARNADVRLRQALRAQLRRCEAAMDGTGDRLIAENERLHSLMYDAARSPQLVQLIDSLG
                     TRLRRARVASLSLADRPVRALEEHRALAAAVIARDVVKARALAAEHAERGRRAATNWY
                     LEHHRVTGTNGGGRS"
     gene            complement(1094..2257)
                     /locus_tag="VFV20_10840"
     CDS             complement(1094..2257)
                     /locus_tag="VFV20_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003180871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU5288889.1"
                     /translation="MRIVAVREKAVPLGGGISNAYIDFGRMTASVVAVVTDAVVDGRP
                     VIGYGFNSNGRYAASGLLRERFIPRLLGASPEALMDANGPNIDPHRVWDVLMRDEKPG
                     GHGERCVAVGVLDMAIWDIVAKVERKPLSMLLAERYRRAPPDERVAVYAAGGYYAAGK
                     DRAALQQEMREYQAQGYTSVKMKIGGAPLDEDQRRIEAVLEVVRDPGVLAVDANGRFD
                     RATALAYADSLAPLGLRWFEEPGDPLDYALNMEIAGRYSSPLATGENLFSVQDARNLL
                     RYAGMRPDRDILQFDPALAYGLVEYLRIVDAVEAAGWSRRRLVPHGGHQFALHIAAGL
                     GLGGNESYPGVFRPFGGFADGIPVEAGYVRPPDAPGIGYELKSDLYALLRDLH"
     gene            complement(2274..>3023)
                     /gene="tdh"
                     /locus_tag="VFV20_10845"
     CDS             complement(2274..>3023)
                     /gene="tdh"
                     /locus_tag="VFV20_10845"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005501465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEU5288890.1"
                     /translation="GHCRNCRAGKRHLCRNTQGIGVNRAGAFAEFIAVPASNAYVLPP
                     GMPDEIGSCLDPLGNAVHSALSFELAGEDVLITGAGPIGVMSAAVSRFVGSRYVVVTD
                     SNHYRLNLARRMGATLAVDYRDTTLDAVMKELGMLEGFDIGLEMSGSPEALRSMLKFM
                     NHGGHVALLGIPSQQIAIDWDQVIFRGLTLKGIYGREMFETWYRMGSMLQSGLDITPV
                     ITHRFAAQDFDEAFAVVRSGQCGKVVMDWTG"
BASE COUNT          438 a         1065 c         1049 g          471 t
ORIGIN      
        1 acgcatacgc gaccgcgccg ctgccgcccg gcttgttcac gacgttgatg ggcttcgccg
       61 acagcttctc cttcgtcagc acgtcggcga tcttgcgggc gaagatgtcg ctgccgccgc
      121 cggccgccgc ctgcacgacg agctcgacgt ccttcgtcgg ggtccaggtg ggcgccggcg
      181 tgctcgtggc agccgctggg gcgctggtcg cgggtgcggg tgtcgggctc gccgtcgatc
      241 cggcgccgcc gcacgcacct gcgacgagac cggcggcgac cgccagggtc acgaaggttc
      301 gtgtgatcct catgaacgtc ctcctccatt tgtgcccgtg acgcggtgat gctcgagata
      361 ccagttcgtc gccgcgcgcc ggccgcgctc ggcgtgttcg gccgcgaggg cgcgggcctt
      421 caccacgtcg cgcgcgatga ccgcggcagc gagagcgcgg tgctcttcga gcgctcgcac
      481 cggccggtcc gcgagcgaga ggctcgcgac gcgcgcgcga cgcagacgcg ttccgagact
      541 gtcgatgagc tgcaccagct gggggctgcg cgccgcgtca tacatgagcg agtgcagtcg
      601 ctcgttctcg gcgatgagcc ggtcgccggt gccgtccatc gcggcctcgc agcggcggag
      661 ctgtgcgcgt agcgcctgcc ggagccgcac atccgcgttg cgcgccgccc gctgcaccgc
      721 gagcacctcg agcgcgccac ggatctcgaa cagctcatcg atctcggcga gcgtgatgtc
      781 ggacacctgt gcgccgacgt gcggcaggac cgtgacgagt ccctcggcct ggagccagcg
      841 gaatgcttcg cgtaccggcg ttcgactcac gccgtgtcgt cgcgcgacct cctcttcgcg
      901 cagtcgcgtc cctggtccga gctccccgtt ctcgatccgc cggaggatcg cgccatggac
      961 ccggctgacc gccgtcgtcc gcgcgggcct cgcgacgctc agagattgca tacagcggaa
     1021 gccagtattg catacgattc tgagaacgga cgaggcggcc gtcgtgtgcc cgcgtgtcgc
     1081 cgccgcgcgg ggcttagtga aggtcgcgca gcagcgcgta caggtcggac ttgagctcgt
     1141 agccgatgcc gggcgcgtca ggcggccgga cgtatccggc ttccacgggg ataccgtccg
     1201 cgaagccgcc gaacggacgg aacacgcccg ggtacgactc gttgccgcca aggccgaggc
     1261 ccgccgcgat gtgcagcgcg aattgatgcc cgccgtgcgg aacgagacgg cgtcgcgacc
     1321 agccggccgc ctcgacggca tcgacgatcc ggaggtactc gacgagcccg tacgcgagtg
     1381 ccggatcgaa ttggaggatg tcccggtcgg ggcgcatgcc cgcgtagcgg aggagattgc
     1441 gggcgtcctg cacggagaag aggttctccc cagtcgcgag cggcgaggag tagcggcccg
     1501 cgatctccat gttcagcgcg tagtcgagcg gatcgcccgg ctcctcgaac cagcggaggc
     1561 cgagcggcgc gagcgaatcc gcgtacgcga gcgcggtcgc cctgtcgaat cgaccattgg
     1621 cgtcgacggc gagcacgccg ggatcgcgca ccacttccag cacggcctcg atgcgccgtt
     1681 ggtcctcgtc gagcggcgcg ccgccgatct tcatcttgac gctggtgtag ccctgcgcct
     1741 ggtactcgcg catctcctgc tgcagcgccg cgcggtcctt accggccgcg tagtacccgc
     1801 ccgcggcgta cacggcgacg cgctcgtccg gtggcgcacg gcgatagcgt tccgcgagaa
     1861 gcatggagag cggcttccgt tcgaccttgg cgacgatgtc ccagatcgcc atgtcgagca
     1921 cgccgacggc cacgcaccgc tcgccgtgcc cacccggttt ctcgtcgcgc atgagcacgt
     1981 cccacacgcg gtgcggatcg atgttcggtc cgttggcatc catcagcgct tctggtgacg
     2041 ccccgagcag ccgcgggatg aaccgttcgc gcagcagccc gcttgccgcg tagcgcccgt
     2101 tggagttgaa gccgtagccg atgaccggcc ggccgtccac gaccgcgtcg gtgacgacgg
     2161 cgacgactga cgcggtcata cggccgaagt cgatgtacgc gttgctgatg ccgccgccga
     2221 gcgggacggc cttctcgcgc acggcgacga tccgcagcgc gggccgcaac gctctagccg
     2281 gtccagtcca tgacgacctt gccgcactgg ccggagcgga cgaccgcgaa cgcctcgtcg
     2341 aagtcctgcg ccgcgaagcg gtgcgtgatg accggcgtga tgtcgaggcc gctctgcagc
     2401 atcgatccca tgcggtacca ggtctcgaac atctcccgtc cgtagatccc cttcagcgtg
     2461 aggccgcgga agatcacctg atcccaatcg atcgcgatct gctggctcgg gatgcccagc
     2521 agcgcgacgt ggccgccgtg gttcatgaac ttcagcatcg accgcagcgc ctccgggcta
     2581 ccggacatct cgaggccgat gtcgaagccc tcgagcatgc cgagctcctt catcaccgcg
     2641 tcgagcgtcg tgtcgcggta gtcgaccgcg agcgtggcgc ccatccgtcg cgccagattc
     2701 agccggtagt ggttgctgtc ggtcaccacg acgtatcgcg acccgacgaa gcgactcacc
     2761 gcggcggaca tcacgccgat gggacccgcg ccggtgatca ggacgtcctc gccggcgagc
     2821 tcgaaggaga gcgcgctgtg caccgcgttc cccagcggat cgaggcacga gccgatctcg
     2881 tcgggcatgc ccggcggcag gacgtacgcg ttggacgcgg gcaccgcgat gaactcggcg
     2941 aacgctcccg cccggttcac gccgatgccc tgggtgttgc ggcagaggtg ccgcttgccg
     3001 gcgcggcagt tgcggcagtg gcc
//
LOCUS       DASTZR010000488         2028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_858644, whole genome shotgun sequence.
ACCESSION   DASTZR010000488 DASTZR010000000
VERSION     DASTZR010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858644"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..1302)
                     /locus_tag="VFV20_10850"
     CDS             complement(199..1302)
                     /locus_tag="VFV20_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5288891.1"
                     /translation="MKFPTFEMERMQSAWEHRIKYDLSESGVEAMTLEDATRDARELM
                     RTKLGYAEGVGRDTTRALVASFHSGTSVENVVITTGTSEANFLAITTLVSPGDEVVIV
                     MPNYMQLHGLAIGLGARVREVWLREERGWTIDLDALAASVNAKTKLICVCTPNNPTGQ
                     SLTRAEVARVVEIASRHGAWILADEVYRGAELSGEESASFSGRGERVMVTGGLSKVYG
                     LPGLRVGWLVAPADRARAALELKDYTTIAPATLSEILAEVALERRTKLLERARFLVNE
                     RWPALEEWAATHARELHWTPPQAGAICFFSYRFPVDSMAFVDRLIREYSTMVVPGVQF
                     RAERHLRIGFGMEPRLLHAGLAAIDRLLAALGD"
     gene            1328..1951
                     /locus_tag="VFV20_10855"
     CDS             1328..1951
                     /locus_tag="VFV20_10855"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEU5288892.1"
                     /translation="MRRIGHRGAKGHAAENTIASFEKALELGCDEVETDVWLMDDGSL
                     VISHDRPASTHGLLTLDEVLDLARGRMGVNVELKSEGAERRAYETGAVVGARLAERRD
                     DSVYVSSFWWSALEAVRESALSVRRAYLFASSPPRTSLIAEARAVRLWGFHPNRAYVT
                     PDLVREAHAAGLSVQAWTVNDPDEIARFTSWGLDGLMSDYPERIPKR"
BASE COUNT          292 a          710 c          729 g          297 t
ORIGIN      
        1 ggggcgagcg tgtgcacgaa ggtctccgcg agcgccgcgg gcgcggaggc aaggccggcg
       61 aagcgctgtt ccgtgaccag gccggcctcg aacagatgga gcggacgagc cgccatttgt
      121 gggcgcatgc tagccaaacc agcagccgac cggctagtcc tgccctggct ggaggcggta
      181 ccagccgccg gcggcgcgct agtcgccgag ggccgccagg agccggtcga tggcggcaag
      241 gccggcgtgg aggagccgcg gttccatgcc gaagccgatc cggaggtgcc gctccgcccg
      301 gaactggacg cccgggacga ccatcgtcga gtactcgcgg atgagccggt cgacgaaggc
      361 catcgagtcg acggggaacc ggtacgagaa gaagcagatg gcccccgcct ggggcggcgt
      421 ccaatgcagc tcgcgcgcgt gcgttgccgc ccactcctcc agggcgggcc agcgctcgtt
      481 caccaggaac cgggcgcgtt cgaggagctt cgtccgccgc tcgagcgcga cctcggcgag
      541 gatctcggag agcgtcgccg gcgcgatcgt cgtgtagtcc ttgagctcga gcgccgcgcg
      601 agcgcggtcg gccggcgcca cgagccagcc gacccgcaga cccggcagcc cgtacacctt
      661 ggagagcccg ccggtcacca tgacgcgctc gccgcggccg ctgaacgacg cgctctcctc
      721 gccggagagc tcggccccgc gatagacctc gtcggcgagg atccacgcgc cgtgccgcga
      781 cgcgatctcg acgacgcgcg ccacctcggc gcgggtgagt gactgcccgg tggggttgtt
      841 cggcgtgcag acgcagatga gcttcgtctt cgcgttcacc gaggccgcga gagcgtcgag
      901 atcgatggtc cagccgcgct cctcgcgcag ccacacctcg cgcacgcggg cgccgagccc
      961 gatcgcgagc ccgtggagct gcatgtagtt cggcatgacg atcacgacct cgtcgccggg
     1021 tgacacgagc gtcgtgatgg cgaggaagtt cgcctcgctt gtgccggtcg tgatgacgac
     1081 gttctcgacg ctcgtcccgc tgtggaagct cgcgacgagg gcgcgcgtcg tgtcgcgccc
     1141 gacgccctcg gcgtagccca gcttcgtgcg catgagctcg cgcgcgtcgc gcgtcgcgtc
     1201 ctcgagcgtc atcgcctcga cgcccgactc ggagaggtcg tacttgatac ggtgttccca
     1261 cgcggattgc atccgttcca tctcgaacgt cgggaacttc atcgcgggcc acgatacgct
     1321 gacgcgcgtg cgacgcatcg gccatcgcgg cgcgaagggc cacgccgcgg agaacaccat
     1381 cgcctcgttc gagaaggcgc tcgagctcgg ctgtgacgag gtcgagacgg acgtctggct
     1441 catggacgac ggatcgctcg tcatatcgca cgaccgcccc gcgtcgacgc acgggctcct
     1501 gacgctcgat gaggtactgg atctcgcgcg cggccggatg ggcgtgaacg tcgagctcaa
     1561 gtccgagggt gccgaacgtc gcgcgtacga gaccggcgcg gtcgtgggcg cgcggctcgc
     1621 cgagcggcgc gacgactccg tgtacgtctc gagcttctgg tggtcggcgc tcgaagcggt
     1681 ccgcgagagc gcgctgtcgg ttcgacgtgc gtatctcttc gcctcgtcgc cgccgcgcac
     1741 gtcgctcatc gcggaggcgc gcgcggtccg actctggggt ttccatccga atcgggccta
     1801 cgtcacgccg gacctcgtgc gcgaggcgca cgccgcgggg ctctccgttc aggcctggac
     1861 ggtcaacgat ccagacgaga tcgcccggtt cacctcgtgg ggcctcgacg ggctcatgtc
     1921 cgattacccg gagcggatcc cgaagcgctg acccagtcgc gcgcccgcgc cacggcgcgg
     1981 cgccatccgt cgtagcgcgc ctcgcgctcg tcggcggaga tgcgcggc
//
LOCUS       DASTZR010000489         4804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_204922, whole genome shotgun sequence.
ACCESSION   DASTZR010000489 DASTZR010000000
VERSION     DASTZR010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204922"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..2092
                     /locus_tag="VFV20_10860"
     CDS             164..2092
                     /locus_tag="VFV20_10860"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEU5288893.1"
                     /translation="MTADDIRGRGERRLADARERLARLETSAPREAAALLRAVDELQL
                     AMTNIASEVSVLVNVHPDLAAREACEAIQREATTLSVRVSQSRPVYDALSGIDIAALD
                     GPARRAVELTLRDMRRAGVALDEEHRRRAQEIRTRITALSQDYARNIRDDVRTVELPA
                     SALDGLPADYRASHPADALGMVKVTTDPPDHGPLMTYSRDKAARLALSRAYYDRAPGN
                     VTVFEQLRSARHDLAVTLGYADFAAYNLEDKMMATPANVDRFLDEVRDAARAPAEEQY
                     AALLSIAKGDDPAASGIGNWDIQYHLARAKDERFRFDPREVRPYLEYRSVRQAILDLA
                     AELFGLVFTPIEPDETWHESVEHFSVTMDGRPAGFISLDMHPRPGKNKWFTSFGYRLG
                     LRGRQLPHNVLVCNFPDPNGQRGPALMEHQQVVTFFHEFGHLVHGIVRGGTVEWARLG
                     RPAENDFMEAPSRFLEDYIFELDVLRRFAKHVETGAPIPQDLVDRLRAARDFGRGVQV
                     EQSASQSRQSLKLHQGDPRRTDARTLAREWAEREQRFAYLDGSNWPVNWEHMGNEAYA
                     AAYATYLWSDTIAADIRTGFAKGLMDTAAARRYRDIVLAPGGNIPAADAVMGFLGRPY
                     NTTAFKDRIAKSGSPPSE"
     gene            2225..>4804
                     /locus_tag="VFV20_10865"
     CDS             2225..>4804
                     /locus_tag="VFV20_10865"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU5288894.1"
                     /translation="MPDSSLVEQRKLVTVLFADLAASTTLGETRDPEDTRAIYERYFA
                     ALARQVRRYDGTIDKYVGDAVMAVFGAPVSHEDDAERAIRAGLAMQAAIANLNDDIER
                     AHDLRLALRVGIYTGEVVAGLLASDVQHAYTVVGDAVNTADRFQKAAPPGEVVVSDMT
                     RRLAREAFVFEALPPVAMKGKAVPAVPFRVVRPRDEEVLPELGRMVDAASELERLRSA
                     FGEAVRGHGRVIDVTGEPGVGKSRLLLEFRRDLPIGVQRVVVRCASYQVTTPYAALAD
                     LVRGSFGIHASDPAGVAREKLVEGAARFRTYLSPELLTLLLGLLGFGERTAPAADLVR
                     QLLVAATRDLLSNVTADAPLVIVFEDVQWMDRPTAAVVSDIALDVPQYACLLVTTSRA
                     SFPLEWRAERIELSPLADMDARALAKDLGADDISMELVERTLERTGGNPFFIEEVLRQ
                     LRDVGGTDVPATVQEVIQAHLDRLPTGPRHVIDVAAVIGRSFWLPLLRRLVSSKTLSE
                     DLASLEQEGFLLRRTMRPELTYAFRQPLLQEVAYHAQLLSARRQLHARVAAAIESLYA
                     ERIEEFTDVMAYHWARTDDHAKALEWLVRAGDRARSLYANDDALASYRAALERAPDGA
                     TPLAAATILERIADVELLVGRYDDAIEACANAWRRARTTDAVLHARLVRKTGAAHRWK
                     GDYAASLAALEQARATLDGADAAEAAHIALEEGMTQFHRGDYAGARAALERGTGLGRR
                     AQLGDALAEGLKILGNVANNLGDLAAAMDAYQQSLTIYERLGDTKGIADVRSNIGNMH
                     RRAGRYEDALAEHRRSLALRERIGHRWGVATNYNNIGEVFRSMGRPADAIPEFERALE
                     TWDS"
BASE COUNT          768 a         1665 c         1654 g          717 t
ORIGIN      
        1 cgaccctctc gccgtcgacg aggacgtcgg ccttgtacgt gtccgccgcg gtcacgatcg
       61 tgccgccggt gatcagcgtc gttgccatgg ctcccctccg aacggcggga tgctaccccc
      121 tacactcgga gccatcaccg agaccgctac tcccagccga cgcatgaccg ccgacgacat
      181 ccgtgggcgg ggcgaacgcc gcctcgcgga tgcgcgggag cggctcgcac ggctcgagac
      241 ttctgcgcca cgggaggccg cggcgctgct gcgtgccgtc gacgagctgc agctcgcgat
      301 gacgaacatc gcgagcgagg tctcggtcct cgtgaacgtc caccccgatc tggcggcgcg
      361 cgaggcgtgc gaagcgatcc agcgcgaggc gacgacgctt tcggtgcggg tctcgcagtc
      421 gcggcccgtg tacgacgcgc tctcgggcat cgacatcgcc gcccttgacg gtcccgcgcg
      481 gcgcgccgtc gagctgacgc ttagggacat gcggcgcgcc ggcgtcgcgc tcgacgagga
      541 gcaccgcagg cgcgcgcagg agatacgcac acgcatcacc gcgctgtcgc aggactacgc
      601 acgcaacatc cgcgacgacg tgcgcacggt cgagctgccc gcatccgcgc tcgacggcct
      661 gcccgccgac tatcgcgcgt cacatcccgc ggacgccttg ggaatggtca aggtgacgac
      721 cgatccgccc gaccacggtc cgctcatgac gtactcgcgc gacaaggccg cgcgtctggc
      781 gctgtcacgc gcgtactacg accgcgcgcc gggcaacgtc accgtcttcg agcagctgcg
      841 ctcggcccgg catgacctcg cggtcacgct cggctacgcc gacttcgccg cctacaacct
      901 cgaggacaag atgatggcga cacccgcgaa cgtggaccgc ttcctcgacg aggtccgcga
      961 cgcggcacgc gcccctgccg aggagcagta cgcggcgctc ctgagcatcg cgaaaggtga
     1021 cgacccggcc gcgagcggca tcggcaactg ggacatccag taccacctcg cgcgcgcgaa
     1081 ggacgagcgg ttccggttcg atccgcgcga ggtacgcccg tatctcgagt accgctcggt
     1141 gcgccaggcc atcctcgacc tcgcggctga gctgttcggc ctggtgttca cgccgatcga
     1201 gccggacgag acgtggcacg agagcgtcga gcacttctcg gtcaccatgg acggcaggcc
     1261 cgccggattc atctcgctcg acatgcatcc gcggccgggc aagaacaagt ggttcacctc
     1321 gttcggctac aggctgggcc tgcgcgggcg gcagctgccg cacaacgtgc tggtgtgcaa
     1381 cttcccggac ccgaacgggc agcgcggtcc cgcgctcatg gagcaccagc aggtggtgac
     1441 gttcttccac gaattcggcc acctcgtcca cggcatcgtg cgcggtggca ccgtcgagtg
     1501 ggcgcggctc ggccggcccg cggagaacga cttcatggag gcgccgtcgc gcttcctcga
     1561 ggactacatc ttcgagctgg acgttctgcg gcgcttcgcg aagcacgtcg agaccggcgc
     1621 gccgataccg caggacctcg tggatcggct gcgggccgcc cgcgacttcg gccgcggcgt
     1681 gcaggtcgag cagagcgcat cgcagtcgcg gcagtcgctc aagctgcacc agggcgatcc
     1741 gcgccggacg gacgcacgga cgctcgcccg cgaatgggcc gaacgtgagc agcgcttcgc
     1801 gtatctcgac ggatcgaact ggccggtgaa ctgggagcac atgggaaacg aggcctacgc
     1861 cgccgcctac gccacgtatc tctggagcga cacgatcgcg gcggacatcc gcaccgggtt
     1921 cgcgaagggt ctgatggaca cggcggccgc gcgccgctac cgcgacatcg tcctcgcgcc
     1981 gggcggcaac atcccggcgg ccgacgcggt gatgggcttc ctcggccgtc catacaacac
     2041 gacggcgttc aaggaccgca tcgcgaagag cgggtcgcca ccctccgagt agtctttccc
     2101 ggtcatggcg gggcaggcgg cgtcgcggca ctgtccgagc tgtggcgcga caacgcgaca
     2161 caacgatcgc ttctgcgcgt catgcggacg ctcgctgacg agcgcgtcgc tgcagctcgg
     2221 cgacgtgccc gactcgtccc tcgtcgagca acgcaagctc gtcacggtgc tattcgccga
     2281 cctggccgcc tcgacgacgc tcggtgagac ccgggacccg gaagacacgc gagcgatcta
     2341 cgagaggtac ttcgccgcgc tcgcccggca ggtgcgccgc tacgacggga cgatcgacaa
     2401 gtacgtcggt gacgcggtca tggccgtatt tggcgcgccg gtctcacacg aggacgacgc
     2461 cgaacgcgcg atccgtgctg gcctcgcgat gcaggccgcg atcgccaact tgaacgacga
     2521 catcgagcgc gcacatgacc tgcgtctggc gttgcgggtc ggcatctaca ccggcgaggt
     2581 cgtcgcgggg ctgctcgcga gcgacgtgca gcacgcctac accgtcgtgg gcgatgccgt
     2641 gaacacggca gacaggttcc agaaggcggc accgcccggc gaggtcgtgg tgagcgacat
     2701 gacacgtcgt ctcgcgcggg aggcgttcgt gttcgaggct ctgccgccag tcgcgatgaa
     2761 gggcaaggcg gtgccggccg tgcccttccg cgtcgtgcgt ccgcgagacg aggaggtcct
     2821 gccggagctc gggcgcatgg tcgacgcggc gagcgaactc gagcgtctgc ggtcggcatt
     2881 tggggaagcc gtccgcggcc acggccgggt gatcgacgtc accggcgaac cgggtgtggg
     2941 gaaatcgagg ctgctgctcg agttccggcg agacctcccg atcggcgtcc agcgtgtcgt
     3001 cgtccgctgc gcgtcgtacc aggtgacgac gccctacgcc gcgctcgcgg acctcgtccg
     3061 tggctcgttc gggatccacg cgagcgatcc cgccggcgtg gcacgcgaga agctcgtgga
     3121 gggtgcggcg cgctttcgca cgtacctcag ccctgaactc ctgacgctgc tgctcgggct
     3181 cctcggtttc ggcgagcgga ccgcgcccgc agctgatctc gtccgtcagc tccttgtcgc
     3241 tgccacgcgc gatctgttgt cgaacgtgac ggccgacgca cccctggtga tcgtcttcga
     3301 ggacgtccaa tggatggacc ggccgacggc cgcggtcgtc agcgacatcg cgttggacgt
     3361 gccgcagtac gcatgtctgc tcgtgacgac gtcacgagcg tcatttcctc ttgagtggcg
     3421 agccgagcgg atcgagctct cgccgctcgc cgacatggat gcgcgggcgc tcgcgaagga
     3481 tcttggtgcc gacgatattt cgatggagct ggtcgaacgg accctcgagc ggacgggcgg
     3541 caatccgttc ttcatcgaag aggtgctgcg acagcttcgc gacgtcggtg gcacggacgt
     3601 gccggcgacg gtgcaggagg tcatccaggc gcacctcgat cggctaccga ccggcccgcg
     3661 ccacgtcatc gacgtagccg ccgtgatcgg tcgttccttc tggctcccac tcctgcggcg
     3721 cttggtctcc agcaagacgc tgagcgagga cctcgcgtcg ctcgaacagg agggattcct
     3781 cctccgaagg accatgcgac ctgagctcac ctacgcgttc cgccagccac tgttgcagga
     3841 ggtcgcgtac cacgcccagc tcctgtcggc acgtcgccag cttcatgcgc gcgtcgccgc
     3901 ggccatcgaa tcgctctacg ccgaacggat cgaggagttc acggacgtga tggcgtacca
     3961 ctgggcgcgc actgacgacc acgcaaaggc actcgagtgg ctcgtgcgcg ccggtgatcg
     4021 ggcccggtcg ctgtacgcca atgatgacgc gctcgcctca tatcgtgccg cgctcgaacg
     4081 cgcgcccgac ggcgcgacgc cgcttgccgc cgcgacgatc ctcgagcgga tcgcggatgt
     4141 cgagctcctc gtcggccggt acgacgatgc gatcgaggca tgcgcgaatg cgtggaggag
     4201 agcacgcacc acggacgccg tcctgcacgc gcgtctggtg cgaaagacgg gcgcggcgca
     4261 tcgctggaag ggtgactacg cggcgtcgct ggcggcgctc gaacaggcac gtgcgacgct
     4321 cgacggagcc gacgccgcgg aggccgcgca tatcgctctc gaggagggca tgacgcagtt
     4381 ccatcgcggc gactacgcgg gggcgcgcgc ggcgctcgaa cgcggcaccg gtcttggcag
     4441 acgcgcacag ctcggtgatg cgctggccga gggcctcaag atcctgggga acgtggcgaa
     4501 caatctcggg gatctcgccg ccgcgatgga cgcctatcag cagagcctca cgatctacga
     4561 gaggctcggc gatacgaagg gcatcgccga cgtgcgaagc aacatcggca acatgcaccg
     4621 acgcgcgggc cgctacgagg acgcgctcgc ggagcatcgt cgcagcctcg cgctgcgcga
     4681 gcgcatcggt catcgctggg gcgtcgcgac gaactacaac aacatcggcg aggtcttccg
     4741 aagcatggga cggccggcgg acgcgatccc cgagttcgag cgcgcgctcg aaacgtggga
     4801 ctcc
//
LOCUS       DASTZR010000490         3712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_988250, whole genome shotgun sequence.
ACCESSION   DASTZR010000490 DASTZR010000000
VERSION     DASTZR010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988250"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..1464
                     /locus_tag="VFV20_10870"
     CDS             220..1464
                     /locus_tag="VFV20_10870"
                     /inference="COORDINATES: protein motif:HMM:NF013950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G1 family glutamic endopeptidase"
                     /protein_id="HEU5288895.1"
                     /translation="MTPDVPGTRPRARRAAPAVAIAALLALALITLGAIDVSRLVSQI
                     APNVAQPASAVADVAAADAVKAAVQRSNEAQASAFNGGDPSAMRAYATDSFYQQLATT
                     NRDLAASGVVRIELVAIDWGDVSVDGASAKATTFETWRSTYRDGSSDETTARNDYVLV
                     SESGAWKVSSDEQPSAALRPAPQTPTDTGSPAAARVFSTSSNWSGYAATGGSFTSVTG
                     TWTVPNVSATTSGADATWVGIGGVESRDLIQAGTQATVGGGDVEYHAWIEMLPAASRP
                     VSLSVSPGDSVTVTITQQTGDSWTITIRNNTTGGRYATTVQYRSTNSSAEWVQEAPSI
                     GRGTVALDDFGAVRFTDASAVRGGRQQTLAALGAEAITMINGSRQALATPSVIGDDGA
                     SFTVTRTSAQATEPGTQRRRRG"
     gene            1553..1807
                     /locus_tag="VFV20_10875"
     CDS             1553..1807
                     /locus_tag="VFV20_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288896.1"
                     /translation="MVTWEMPDGTEFRYQGVAVSDAALREFVLRFMSAEAMSWDVSKW
                     DDKALELAFLRRFGERIRITRERALEGTSVLILQPVMAGA"
     gene            1862..2923
                     /locus_tag="VFV20_10880"
     CDS             1862..2923
                     /locus_tag="VFV20_10880"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5288897.1"
                     /translation="MKTASGYEIVPVDTDVLSDAELRKAAELMQSMNLERVPEDPPTP
                     TEVIMQRLRAKTPSDWRALFAARDPSGALVGSAFVGYNTNEPENAHARWTEVNVLPAH
                     RRRGLGRALLARAVGACLDQRDDLVFFGQTSDRVGAGESFARAVAATPGLPMKLNQLT
                     IADVDRAKVAEWAKLDPAGYRLERSDGRTPDALVQPYLDAVNAMNDMPKGELRFADQR
                     FTEEQLRERESWLAQAGIQRWVIVAIHEATGEGAGFTEISYDPRVPHLLQQGGTGVTR
                     AHRGHRLGLWMKAVMLDRVMRERPAAKYIRTGNADVNKNMLEINTLLGFRHAWSNTLW
                     QLPVAEARASLRLREDAAV"
     gene            complement(3026..3586)
                     /locus_tag="VFV20_10885"
     CDS             complement(3026..3586)
                     /locus_tag="VFV20_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288898.1"
                     /translation="MKTRRPSTGALPSFRSSSALQPSVDVPHRPRPISLRVVGWTKAE
                     RSGSLADHVLVHTSAAHAGAVRPSSRASSTGSKERRSPAARRKRRSPFTTTETPGSSV
                     GSGGADGSATLAAALLVGDALAVVRAEPTGVALAGTVVRAVAVAGAVVVAGFVDATAV
                     AGEVTAGCSTVGPPHDASVAHARMTR"
BASE COUNT          557 a         1340 c         1321 g          494 t
ORIGIN      
        1 cgaagtccgg ctccccggcc cgcccgtcga ccggtgcgcg tgatggaccg caccgtacgg
       61 gcgtccggga ccccacggac ttcgcttcgg tgagaacgac gccctgcgcg tgccacccgc
      121 cgccacgcgg atgtgaccga cgtgtcggga gcgcgcggcc cggtgacgtt tccggcggtc
      181 tctcatccgc ctcttatctc ggcgcgcgaa ccttctggca tgacaccaga cgtaccgggc
      241 acgcgtcctc gggcgcggcg tgccgcgccg gcggtcgcga tcgcggcgct cctcgcgctc
      301 gcgctcatca cgctcggcgc gatcgacgtg agccgccttg tgagccagat cgcgccgaac
      361 gtggcgcagc cggcgtccgc cgtggcagac gtcgcggcgg cggacgcggt gaaggccgcg
      421 gtacagcgct cgaatgaggc gcaggcgagc gcgttcaacg gcggcgatcc gtccgcgatg
      481 cgcgcgtacg cgaccgactc cttctatcag cagctcgcca cgacgaaccg cgatctcgcg
      541 gcatccggcg tcgtgcgcat cgagctcgtg gcgatcgact ggggcgatgt gagcgtggac
      601 ggcgcatcgg cgaaggccac gacgttcgag acgtggcgct ccacctatcg cgacggttcg
      661 agcgacgaga ccaccgcgcg caacgactac gtgctcgtga gcgagtccgg tgcgtggaag
      721 gtgagcagcg atgagcagcc cagcgccgcg ctgcggcccg cgccccagac gcccacggac
      781 acgggctcgc ccgccgcggc gcgcgtcttc tcgacgtcct cgaactggtc gggatacgcc
      841 gcgaccggcg gctcgttcac gtcggtgacc ggtacgtgga cggtgccgaa cgtgtccgcg
      901 acgacgagcg gcgccgacgc cacctgggtc gggatcggcg gcgtcgaatc ccgcgacctc
      961 atccaagcgg ggacccaggc gacggtgggc ggcggcgacg tcgagtacca cgcctggatc
     1021 gagatgctcc ccgcggcatc gcgccccgtc tcgctctcgg tgtctcccgg cgactccgtc
     1081 accgtgacca tcacgcagca gacgggcgac agctggacga tcaccatccg caacaacacc
     1141 acgggtggcc gctacgcgac cacggtgcag taccgctcga cgaactcgtc ggccgagtgg
     1201 gtccaggagg cgccgagcat cgggcgcggg accgtggcgc tcgacgactt cggcgcggtc
     1261 cgcttcaccg acgcgtccgc ggtgcgtggc ggcaggcagc agacgctcgc cgcgctgggc
     1321 gccgaggcga tcacgatgat caatggatcg cggcaggccc tggcgacgcc gtcggtgatc
     1381 ggcgacgacg gagcgagctt caccgtcacg cgcaccagcg cgcaggcgac ggagcccggg
     1441 acgcagcgcc gccgccgcgg atagcggctc ccccgtccgg gggagcaaac gcaccccggc
     1501 gggggatgcc ggtccggggg agccgtgcgt agcctcggta tcaggggcgc atatggtcac
     1561 ctgggagatg cccgacggca ccgagttccg ctaccagggc gtagcggtat cggatgccgc
     1621 tctccgcgag ttcgtcctgc gcttcatgtc cgccgaagcc atgtcgtggg acgtctccaa
     1681 gtgggacgac aaggcgctcg agctggcgtt cctgcgccgc ttcggcgagc ggatccgcat
     1741 cacccgcgag cgggcgctcg aaggcacgtc ggtgctcatc ctccagcccg tcatggcggg
     1801 ggcgtagccc gaacgtatcg cgggacatag tcccgttcgt cggcgcggct acgctggccc
     1861 catgaagaca gcaagcggat acgagatcgt ccccgtcgac accgatgtcc tctccgacgc
     1921 cgagctgcgc aaagcggcgg agctcatgca gtccatgaac ctcgagcgcg tccccgagga
     1981 tccgccgaca ccgaccgagg tcatcatgca gcggctccgc gcgaagacgc ccagcgattg
     2041 gcgcgcgctc ttcgcggcgc gcgatccctc gggcgccctc gtcggctcgg ctttcgtcgg
     2101 ctacaacacg aacgagcccg agaacgcgca tgcccgatgg accgaggtga acgtccttcc
     2161 cgcgcaccgc cgtcgcgggc tcggccgcgc gctgcttgcc cgcgcggtcg gggcgtgcct
     2221 cgaccagcgc gacgatctcg tgttcttcgg tcagacgtcc gatcgcgtcg gcgcgggaga
     2281 gtcgttcgct cgtgcggtcg cggcgacgcc ggggctgccg atgaagctca atcagctcac
     2341 gatcgccgac gtcgaccgtg ccaaggtcgc cgaatgggcg aagctcgacc ccgcgggcta
     2401 ccggctcgaa cggtccgacg gcaggacgcc ggacgcgctc gtgcagccgt acctcgacgc
     2461 ggtgaacgcg atgaatgaca tgcccaaggg cgagcttcgg ttcgccgacc aacggttcac
     2521 cgaggagcag cttcgcgaac gcgagagctg gctcgcgcag gccgggatcc aacgctgggt
     2581 catcgtcgcg atccacgagg cgacgggcga aggcgcgggc ttcaccgaga tcagctacga
     2641 cccccgcgtg ccgcatctcc tgcagcaggg cggaacgggc gtgacgcgcg cgcaccgcgg
     2701 gcaccgcctg ggcctctgga tgaaggccgt catgctggac cgcgtcatgc gcgagcgacc
     2761 ggccgcgaag tacatccgca ccggcaacgc ggacgtgaac aagaacatgc tcgagatcaa
     2821 cacgctcctc ggcttccgcc acgcgtggtc caacacgctg tggcagctgc cggtcgcgga
     2881 ggcgcgcgcc agcctgcgac tgcgcgagga cgccgcggtc tagcgaccgg cgcgcgcgtc
     2941 ccgggcctcc cgaccctgtg ccacactcca gccggcacga ggagggagca aggatggccg
     3001 tctcatggat gggggagcga gccgctcacc gcgtcatcct cgcgtgcgcg acgctcgcgt
     3061 cgtgcggcgg gcccacggtg ctgcagccag ccgtgacctc gcccgcgacg gcagtcgcgt
     3121 caacgaagcc cgcgacgacc acggcacccg cgacggcgac cgcgcggacc accgttccgg
     3181 ccagcgcgac gcccgtgggc tccgcgcgga ccacggcgag cgcgtccccg acgagcagcg
     3241 cggcagcgag cgtggcgctc ccgtccgcgc cgcccgagcc gacgctcgag cccggcgtct
     3301 ccgtggtggt gaagggcgag cgtcgcttcc gcctcgctgc cggcgagcgc cgctccttcg
     3361 atccggtcga gctcgcgcgc gaagagggtc gtacggcgcc ggcgtgcgcc gcgctggtgt
     3421 ggacgagcac gtggtccgcg agcgagccac tgcgctcggc tttcgtccag ccgaccacgc
     3481 gaagcgagat cggcctcggc cgatggggga cgtcgacgct cggctgcagc gcgctcgagc
     3541 tgcggaacga cgggagcgcg ccggtcgacg gccgcctcgt cttcacgctg gcctctcgct
     3601 aggagaccac tcgacgcgtc agggtgtgtc gggcgtgggt gcgaccgcgt aacgctcgcc
     3661 gacgaactcc aggaccgcat tcggcgtcac gtcgaggtag acgaactcga tg
//
LOCUS       DASTZR010000491         4394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_74469, whole genome shotgun sequence.
ACCESSION   DASTZR010000491 DASTZR010000000
VERSION     DASTZR010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74469"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VFV20_10890"
     CDS             <1..328
                     /locus_tag="VFV20_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288899.1"
                     /translation="FAVSVSAVVVASLVACLWARAAARGRDRRAGMLSRSLPAIATTF
                     AATTVVASPASLPLLILERERSLEGCGFGTCHGEALLLWIAVLAVGTIAIPAAFASAL
                     HKRSRA"
     gene            365..751
                     /locus_tag="VFV20_10895"
     CDS             365..751
                     /locus_tag="VFV20_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5288900.1"
                     /translation="MEEFAPVGNRVLVVDDDPDIRDLLVSVLQDDGYEAESARNGKDA
                     LDVLERWPADVVVLDLMMPVMDGWTFADRMRERWRIPIVVLSAATDLSRHAKRLGAAD
                     VVPKPFELDALLPRIARVAAAGGSAT"
     gene            762..2267
                     /locus_tag="VFV20_10900"
     CDS             762..2267
                     /locus_tag="VFV20_10900"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU5288901.1"
                     /translation="MGRRAWIGLLLLVVLLPLVLLAWLLASPAANGPWVIPVEHLVVT
                     ANVSIVALLVAVLTARAALQVRHYPTLLIALGFMSMAGIFAVHGLSTPGVIQRGAKAD
                     DAGLVAGVSAQLALWAPALFFTARYTPLAGWLERRVAPAALLAAVLAGLGAYAVAAIG
                     WPALFDGVARWTLVSAGAASDYDPSGYGYGYGNGSGQPDALGGAGAAPYVLATSVVLL
                     FGFAALRQGADYLRTHLPLQGALTASYVFLAQAQISQFLGPTWTPSWWEYHVLMALAT
                     VLALFALFVELDRRRGLERFLPPNVVERVIQGDPLRLEGERQTVTILFADLRGSTALA
                     ERLEPEAIVAVLNTYLRVMAQCVLDAGGILDKFLGDGLMAIFGAMGDPSHGAVAATTA
                     ARDMRARMTALNAERTARGEPVVQFGVGLHTGDVVLGAVGLPQRSDYTAIGDTVNTAS
                     RMESLTKEFTVDSVLSEETAEHLDGGRSGLAPLGEAQVRGKAAKIRVYTLR"
     gene            2278..2661
                     /locus_tag="VFV20_10905"
     CDS             2278..2661
                     /locus_tag="VFV20_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016431904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5288902.1"
                     /translation="MPDLDAIGLVAADMKESVRFYRLLGLDVPDPTDDHLEVSLPNGM
                     RIMFDTVAMAQQFDPQWTRPTGHAFALAFRCASPAEVDATYMKVTKAGFHGKLAPFDA
                     FWGQRYATVLDPDGNAADLFADLKR"
     gene            complement(2692..3615)
                     /locus_tag="VFV20_10910"
     CDS             complement(2692..3615)
                     /locus_tag="VFV20_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019926891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU5288903.1"
                     /translation="MQIGQLRCFLAVADERHFTRAARNLGLAQPSVSAQVRSLETDVG
                     AQLFHRMKGHIALTEAGEALLPFARRILADVDAATSQLQEVGGLARGRLAIGATPSLT
                     AALVPPLLARFHAAYPGIELALREAGSEDLVSALEEGVVDIALVILPVRHEVLETQAL
                     LREELVLAVSRSHQLARRRTIAVADLRDVPLVMFREGYDLRSATEAACRAAGFAPTFA
                     VEGGEMDGVLRLTAAGLGAAIVPSMVVAEHRTLLAVRITQPTLTRTIGIAHRRDRRLS
                     RAAQELVDTVRTLIRDRTWLKTLPPGLTALR"
     gene            3710..>4394
                     /locus_tag="VFV20_10915"
     CDS             3710..>4394
                     /locus_tag="VFV20_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288904.1"
                     /translation="MATIANVRQRPTERRDLWWFEPAAVVIGFTAFVIYAMWAGTTVE
                     GYFYDPYLSPFFSPCITSNCEYLTFGSPWISGWTLAPAIFIVGFPLIFRATCYFFRRT
                     YYRSYFLSPPSCAVPDARHTYSGETKFPFILQNIHRYTWYIAVVVFFVLAYDAVISYR
                     FENGWGIGLGSLIMTAEAVLIGLYTFSCHSCRALCGGWLDSFHGRPFWYRLWMTVSRL
                     NVRHGTFFWV"
BASE COUNT          575 a         1549 c         1514 g          756 t
ORIGIN      
        1 cttcgccgtt tcggtgtccg ccgtcgtggt ggcgtcgctc gtcgcgtgtc tctgggcgcg
       61 cgccgcggcg cgaggccgcg accggcgggc cgggatgctg tcgcgatccc ttcccgcgat
      121 cgccacgacc ttcgccgcga cgacggtcgt cgcgtcgccc gcgagcctgc cgctcctcat
      181 cctcgagcgc gagcgatcgc tggagggctg cggcttcggc acgtgccacg gcgaggcgct
      241 cctgctctgg atagcggtgc tggccgtcgg cacgatcgct atccccgcgg ccttcgcgtc
      301 cgccctgcat aagcgttcga gggcctgacg acagggctcg ctcgctcggc cactgaggcg
      361 ttctgtggaa gaattcgcgc cggtgggcaa tcgggtcctc gtggtggacg acgatcccga
      421 cattcgggat ctgctcgtgt ctgtcctgca ggacgatggc tatgaggcgg aatcggcgcg
      481 caacggcaag gacgcgctcg acgtgctcga gcgatggccg gctgatgtcg tcgtgctcga
      541 tctcatgatg cccgtgatgg acggatggac cttcgccgat cgcatgcgcg agcggtggcg
      601 cattcccatc gtcgtcctct ccgcggcgac ggatctgtcg cgtcacgcga agcggctcgg
      661 cgccgccgat gtcgtgccga agccgttcga gctcgacgcg ctgctgccgc gcatcgcccg
      721 tgtggcggcc gccggaggaa gcgccaccta gactcctccc cgtgggacgg cgtgcgtgga
      781 tcgggcttct gctgctcgtc gtgctcctgc cgctcgtgct gctcgcgtgg ctcctcgcct
      841 cgcccgccgc gaacggccca tgggtcatcc cggtcgagca cctcgtcgtc acggcgaacg
      901 tgtcgatcgt cgccctgctc gtcgccgtgc tcaccgcccg cgccgcgctg caggtccgtc
      961 actatccaac gctcctcatc gcgctcggat tcatgtcgat ggccgggatc ttcgcggtgc
     1021 atggcctgtc gacaccggga gtgatccagc gtggcgcgaa ggcggacgac gccggactcg
     1081 tcgcgggcgt gtcggcgcag ctcgcgctct gggcgccggc cctgttcttc accgctcgct
     1141 acacgccgct cgccggctgg ctcgagcggc gcgtcgcgcc cgcggcgctg ctcgccgctg
     1201 tccttgccgg cctcggcgca tacgccgtcg ctgcgatcgg gtggccggcg ctcttcgatg
     1261 gcgtcgcgcg ctggacgctg gtctccgccg gcgccgcgag cgactacgac ccgtccggct
     1321 acgggtacgg ctacgggaac gggtccgggc agccggacgc gctcggtggt gcgggtgccg
     1381 cgccgtacgt gctcgcgacg agcgtcgtgc tgttgttcgg cttcgccgcg ctgcgccagg
     1441 gcgcggacta cctgcgcacg cacttgcccc tgcagggcgc gctcacggcg tcgtacgtct
     1501 tcctcgcgca ggcgcagatc tcgcagttcc tcgggccgac gtggacgccg tcctggtggg
     1561 agtaccacgt actcatggct ctggcgacgg tcctcgcgct cttcgcgctc ttcgtggagc
     1621 tcgatcggcg ccgtggcctc gagcgcttcc tgccgcccaa cgtcgtcgaa cgtgtgatcc
     1681 agggcgaccc gcttcgcctc gaaggagagc ggcagaccgt gacgatcctc ttcgccgacc
     1741 tgcgcggatc gaccgcgctc gccgagcggc tggagccgga ggcgatcgtg gccgtgctca
     1801 acacctatct gcgcgtgatg gcgcagtgcg tcctcgacgc gggcggcatc ctggacaagt
     1861 tcctcggcga tgggctcatg gcgatcttcg gcgcgatggg cgatccctcc cacggcgccg
     1921 tcgcggccac gacggcggcg cgggacatgc gcgcgcgcat gaccgcgttg aacgcggaac
     1981 gcacggcccg cggcgagccg gtcgtgcaat tcggcgtggg tctgcacacc ggcgacgtgg
     2041 tgctcggcgc tgtgggcttg ccgcagcggt ccgattacac ggccatcggc gacacggtca
     2101 acaccgcgtc gcgcatggag tcacttacga aggagttcac ggtcgacagc gtgctctccg
     2161 aggagaccgc cgagcacctc gacggcggac ggtctggcct cgcgccgctc ggcgaggcac
     2221 aggtccgcgg caaggccgcg aagatccgcg tctacacgct gcggtagcct ttccgccgtg
     2281 ccggatctcg acgcgatcgg cctcgtcgcc gcggacatga aggagtccgt ccgcttctac
     2341 cggctgctcg gcctcgatgt gcccgatccg accgacgacc atctcgaggt ctcgctgccg
     2401 aacgggatgc gcatcatgtt cgacaccgtc gcgatggcgc agcagttcga tccgcagtgg
     2461 acgaggccga ccggacacgc cttcgcgctc gcctttcgtt gcgcgtcacc cgccgaggtg
     2521 gacgcgacct acatgaaggt cacgaaagca ggcttccacg ggaagctcgc gccgttcgac
     2581 gccttctggg gccagcgcta cgcgacggtg ctcgatccag acgggaatgc cgcggacctc
     2641 ttcgcagatc tgaagcgatg agcggcgcgt gccgtcacgc gttgccgcgg ctcacctgag
     2701 cgcggtcagt cccggcggca gcgtcttcag ccacgtgcgg tcccggatga gcgtgcgcac
     2761 ggtgtcgacg agctcctgcg ccgcgcgcga caggcggcgg tcacgccggt gcgcgatgcc
     2821 gatcgtgcgc gtcagcgtgg gctgcgtgat gcgcacggcg aggagcgtgc ggtgctccgc
     2881 cacgaccatg ctcggcacga tcgccgcgcc gaggcccgcg gcagtcagac gaaggacgcc
     2941 gtccatctcg ccgccctcga ccgcgaacgt cggcgcgaag ccggcggcgc ggcaggcggc
     3001 ctcggtcgcg ctgcgcaggt cgtagccttc gcggaacatc accagcggaa cgtcgcgcag
     3061 gtcggcgacc gcgatcgtgc gtcgcctcgc gagctggtgc gagcgcgaca cggcgagcac
     3121 gagctcctcg cgcagcaggg cctgcgtctc gaggacctcg tgacgtaccg ggaggatcac
     3181 cagcgcgatg tccacgacgc cctcctccag agcggagacg aggtcctccg agccggcttc
     3241 gcgcagcgcg agctcgatgc cggggtaggc ggcgtggaag cgggcgagca gcggcgggac
     3301 gagggccgcg gtgaggctcg gcgtcgcgcc gatggcgagc cgtcctcgtg cgaggccgcc
     3361 gacctcctgc agctgtgacg tggccgcgtc cacgtccgcg aggatccggc gggcgaaggg
     3421 gagcagcgcc tcgccggcct cggtcagcgc gatgtgcccc ttcatgcggt ggaagagctg
     3481 cgcgccgacg tcggtctcca ggctgcggac ctgcgcgctc accgagggct gggcgagacc
     3541 gaggttccgt gcggcgcgcg tgaagtgccg ctcgtccgcg acggcgagga agcagcgcaa
     3601 ctggccgatc tgcacgagat ataggctaaa cctatcgagt caacagtcat gatgtatttg
     3661 tcttctgaca gccagagccg tacggtgccc atcgaacacg gaggtgggca tggcgaccat
     3721 cgccaacgtg cgacagcggc cgaccgagcg acgcgatctc tggtggttcg agcccgctgc
     3781 ggtggtgatc ggcttcacgg ccttcgtgat ctacgcgatg tgggccggca cgacggtcga
     3841 gggctacttc tacgacccgt acctgtcgcc cttcttctct ccgtgcatca cctcgaactg
     3901 cgaatacctc acgttcggct cgccgtggat cagcggatgg acgctcgcgc cggcgatctt
     3961 catcgtcggc ttcccgctca tcttccgcgc gacctgttac ttcttccgcc gcacctacta
     4021 ccgctcgtac ttcctctcac cgccgtcctg tgcggtccct gacgcgcgcc acacctacag
     4081 cggcgagacg aagttcccgt tcatccttca gaacatccac cgctacacct ggtacatcgc
     4141 cgtcgtggtc ttcttcgtcc tcgcgtacga cgcggtcatc tcgtaccgct tcgagaacgg
     4201 ctgggggatc ggtctcgggt cgctcatcat gaccgcggag gcggtgctca tcggcctcta
     4261 cacgttctcg tgccactcgt gccgcgccct ctgcggtggc tggctggaca gcttccacgg
     4321 ccgtccgttc tggtaccggc tctggatgac cgtgagccgg ctcaacgtcc gccacggtac
     4381 gttcttctgg gtga
//
LOCUS       DASTZR010000492         2119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_205333, whole genome shotgun sequence.
ACCESSION   DASTZR010000492 DASTZR010000000
VERSION     DASTZR010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205333"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VFV20_10920"
     CDS             complement(<1..676)
                     /locus_tag="VFV20_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288905.1"
                     /translation="MRGVLAVLFVSASLLAGGRPAAAITQLSGFHSAWAGQDPWPSLA
                     AGATTTYSLRFKNTGTETWQRGVAGRQVNLGIVNDSLAFGALAVNWMSANRVATTVED
                     AVAPGAVGTFTFTLRAPAATGVYDLPLRPVVDGVTWLEDQGVFVRVVSDLGYHSKWVS
                     QSPSPTLGPGETSADITLIFRNTGTKTWTKGVLGSQAALGIRNDDRTWASLAVGWPDA
                     TRPAVQA"
     gene            complement(717..1616)
                     /locus_tag="VFV20_10925"
     CDS             complement(717..1616)
                     /locus_tag="VFV20_10925"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5288906.1"
                     /translation="MDVRVVTCEGYRFRVTTWGQGAPHAVVLPGLSADARSLAPQIRE
                     LRARYASVHVVDLPGSVLRPSLMRKDASFPNLARMVGCVADSIGVERAVFVGHSLGGG
                     IALHLALERRELVEALVLLAPAALGRSLHWIYKLYCIPLVGRALLRPQPNVSKPFLRR
                     FLVGSGRAADEHFLEMLLRHGSTSRDRAMSARAIVWANQPAWWRRMLLMLLPGGEQMA
                     FSIADRLGEIAGVPTLVLWGREDRVICVRDAERLRAANPAAEIHVARGVGHMIPLEAA
                     AWANRWIRDFCGRLREEELPRAA"
     gene            complement(1660..>2119)
                     /locus_tag="VFV20_10930"
     CDS             complement(1660..>2119)
                     /locus_tag="VFV20_10930"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5288907.1"
                     /translation="YAEEFARDADAERKFFELVSDVRGDVPATDPPTPETIEEWRVHW
                     TAAPGFIPEACFVAIDAAGRWLGVSNLDRPLDDSGFLWQGLTGVRREARGRGVAMALK
                     LRTVEYARARGVDHIKTWNDQRNRPMLSINEAMGFVKQPAWIALELRLRS"
BASE COUNT          332 a          768 c          748 g          271 t
ORIGIN      
        1 cggcctggac ggccggacgt gtcgcgtccg gccatccgac cgcgagcgaa gcccaggtgc
       61 ggtcgtcgtt gcggatcccg agcgccgcct gcgaccccag gacacccttc gtccaggtct
      121 tcgtgccggt gttgcggaag atgagcgtga tgtccgcgct cgtctcgccg ggtccgagcg
      181 tcggactcgg cgactggctc acccacttgc tgtggtagcc gaggtcgctc accacacgaa
      241 cgaacacgcc ctgatcctcg agccaggtca cgccgtccac caccgggcgg agcggcaggt
      301 cgtacacgcc ggtcgcggcg ggtgcgcgca gcgtgaaggt gaaggtgccg accgcacccg
      361 gcgcgaccgc gtcctccacg gtcgtggcga cgcggttcgc gctcatccag ttcaccgcga
      421 gcgcaccgaa ggcgagcgag tcgttcacga tcccgaggtt cacctggcgg ccggccacac
      481 cgcgctgcca cgtctcggtg ccggtgttct tgaagcgcag cgaatacgtc gtggtcgcac
      541 cggccgcgag cgatggccag gggtcctggc cggcccaggc gctgtggaag ccgctgagct
      601 gggtgatggc ggcggcgggg cgtccgcccg caagcaggga cgcggacacg aagagcaccg
      661 cgaggacgcc tctcaccccg tagagcaacg aagcaggcgg cgtcccgtga cggtccttac
      721 gccgccctcg gcagctcctc ctcgcggagg cgaccgcaga agtcacggat ccaacgattc
      781 gcccacgccg ccgcctcgag cgggatcatg tgaccgacgc cacgcgcgac gtggatctcg
      841 gcggccgggt tcgcggcacg cagccgctcc gcgtcgcgca cgcagatgac gcggtcctcg
      901 cggccccaca gcacgagcgt cggcacgccg gcgatctcgc ccaggcgatc cgcgatcgag
      961 aacgccatct gctcgccgcc cggcaggagc atgagcaaca tgcgccgcca ccacgcgggc
     1021 tgattcgccc agacgatcgc gcgtgccgac atcgcccggt cgcgcgacgt cgatccgtgg
     1081 cgcagcagca tctccaggaa gtgctcgtcc gcggcgcgcc cgctcccgac gaggaagcgc
     1141 cgcaggaagg gcttggacac gttcggctgt ggacgcagga gcgcgcgtcc gacgagcggg
     1201 atgcagtaga gcttgtagat ccagtgcagc gaacggccga gcgccgcggg cgcgagcagc
     1261 acgagcgcct cgaccagctc gcggcgctcg agcgcgagat gcagcgcgat gccgccgccg
     1321 agcgagtgcc cgacgaacac agcgcgctcg acgccgatcg agtcggcgac gcagccgacc
     1381 atgcgcgcga ggttcgggaa ggaagcgtcc tttcgcatga gcgacgggcg cagcaccgaa
     1441 ccggggaggt cgacgacgtg gaccgacgcg taccgcgcgc gcagctcgcg gatctgcgga
     1501 gcgagcgagc gggcgtcggc cgacaggccg gggagcacga ccgcgtgcgg cgcgccctga
     1561 ccccatgtcg tcacgcggaa gcggtagccc tcgcaggtga cgacccgtac gtccatgccg
     1621 tcgcgaatgc acctggcatg ccgcctgcct cagcgacggc tacgagcgga gccgcagctc
     1681 cagggcgatc catgcgggct gcttcacgaa gcccatcgcc tcgttgatcg agagcatcgg
     1741 gcggttgcgc tggtcgttcc acgtcttgat gtgatcgacg ccacgcgccc tcgcgtactc
     1801 caccgtcctg agcttcagcg ccatcgccac accccggccg cgcgcctcgc ggcgcacgcc
     1861 ggtgagcccc tgccagagga agccggagtc gtcgagcgga cggtccaggt tggagacgcc
     1921 gagccagcgt cccgccgcgt cgatcgcgac gaagcacgcc tcggggatga agccgggcgc
     1981 cgccgtccag tgcacgcgcc actcctcgat cgtctcgggc gtcggcgggt cggtcgcggg
     2041 gacgtcgccg cgaacgtcgg agacgagctc gaagaacttc cgctcagcgt ccgcgtcgcg
     2101 cgcgaactcc tccgcgtac
//
LOCUS       DASTZR010000493         2692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_205707, whole genome shotgun sequence.
ACCESSION   DASTZR010000493 DASTZR010000000
VERSION     DASTZR010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205707"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VFV20_10935"
     CDS             complement(<1..307)
                     /locus_tag="VFV20_10935"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEU5288908.1"
                     /translation="MTLRHPDAIGSIVIALVAFLVIAAGLATPDPGFGVVGPAAFPIA
                     IGALMLIVAAWLGWDTWRHAVPMVLDPIDRRPFVSSLVATAAFLLAFEPVGFMISGAA
                     "
     gene            complement(304..1332)
                     /locus_tag="VFV20_10940"
     CDS             complement(304..1332)
                     /locus_tag="VFV20_10940"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEU5288909.1"
                     /translation="MRTRIAAVVTTAALILTACGGGGTGATNTSATPATSAAASAASS
                     YPTKPIDIMAPAGTGGGFDTTARLIQKALTDDKIVTQNVSVSNKPGAGGTVGMAELIK
                     NNAANPYALIVVGRVTLGSQITGKTEAKVSQLTPIARLMAESEVIVTPKDSPIKSMKD
                     LMDALKKDAKAVKFAGGSAGGIDHELAGRLVKLATGDIKGLSYVPFAGGGEAAAAIIG
                     GQVQVGISGFGEFKGHIESGAMKALAVSADAPLASAKTVPTLKSQGVDVVMVNWRLLA
                     APPNIPAGDLAALKAIATKVHDSKTWMDFAEKNDWFDNFTTEGLLTFISAETQSATVT
                     LTDLGLVK"
     gene            1383..>2692
                     /locus_tag="VFV20_10945"
     CDS             1383..>2692
                     /locus_tag="VFV20_10945"
                     /inference="COORDINATES: protein motif:HMM:NF028512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HEU5288910.1"
                     /translation="MGATLQGIRAQWSLWSFWSRTLARQLLLLQIAVVAVTVVVGALA
                     SYGLVSNQIDEQYKQRALAIGYAVAATPDVLDAMDEPEPARVIQPIAEAIRRSVGADF
                     VVVANRDGIRYSHPNPARIGERVSTDPGPALAGNPFVGVEEGTLGRSLRAKVPITKNG
                     EVIGIVSVGFLEEQVAQKLAQALPTMTLTVLLALTLGVAGSLILARRIERQTFGLGTR
                     EIAGLLEQRDAMLHGIREGVLALDTSGRVTVANDEARRLLGITGTVEGRRLAEVVPEG
                     RAREVLGGSTTGNDQVVLAGDRVLVLNRMPVAVRGREVGAVVTLRDRTELEGVLRELD
                     TVRGLAQALRAQTHEFSNKLHVVAGLIELGRLEEAVRFIAKTSLVHQELVELVQQRIA
                     DPALAALLLAKAALASERRVDLRVASDAHLPRDLADVRDLVTVVG"
BASE COUNT          385 a          937 c          951 g          419 t
ORIGIN      
        1 acgcggcgcc gctgatcatg aagccgaccg gctcgaacgc caggaggaag gcggcggtcg
       61 cgacgagcga gctgacgaag gggcgccggt ctatcgggtc gaggaccatc ggcaccgcgt
      121 gccgccaggt gtcccaaccg agccaggcag caacgatgag catgagcgcg ccgatggcga
      181 tcgggaaggc cgccggcccg acgaccccga atccaggatc cggggtcgcc aggccggccg
      241 cgatcacgag gaacgcgacc agcgcgatga cgatcgagcc gatcgcgtcg gggtgccgaa
      301 gggtcacttg accaggccga gatccgtgag ggtcaccgtg gcggactgcg tctccgcgga
      361 gatgaaggtg agcaggccct ccgtcgtgaa gttgtcgaac cagtcgttct tctccgcgaa
      421 gtccatccag gtcttgctgt cgtggacctt ggtagcgatg gccttcagcg cggcgaggtc
      481 gcccgcgggg atgttcggcg gcgccgcgag aagacgccag ttcaccatga cgacatccac
      541 gccctggctc ttcagcgtgg ggaccgtctt cgccgacgcg agcggcgcgt cggccgagac
      601 ggcgagcgcc ttcatcgcgc cgctttcgat gtggcccttg aattcgccga agccgctgat
      661 gccgacctgc acctgaccgc cgatgatcgc cgcggcggcc tcgccaccgc cggcgaaggg
      721 cacgtacgag agacccttga tgtcaccggt cgcgagcttc acgagacggc cggcgagctc
      781 gtggtcgatg ccgccggccg atccgccggc gaacttcacc gccttggcgt ccttcttcaa
      841 cgcgtccatg aggtccttca tcgacttgat gggcgaatcc ttcggcgtga cgatgacctc
      901 ggactccgcc atgagacgcg cgatcggcgt gagctgcgac accttcgcct cggtcttgcc
      961 ggtgatctgc gagccgagcg tcacgcggcc gacgacgatg agcgcgtacg gattggccgc
     1021 gttgttcttg atgagctcgg ccatgccgac cgtgccgccg gcgcccggct tgttggacac
     1081 ggacacgttc tgcgtgacga tcttgtcgtc ggtcagcgcc ttctggatga gccgtgcggt
     1141 cgtgtcgaag ccgccacccg ttccggccgg ggccatgatg tcgatcggct tcgtcgggta
     1201 gctgctcgcc gcgctcgcgg cggcgctcgt cgcgggcgtg gccgatgtgt tggtcgcgcc
     1261 ggtcccgccg ccgccgcatg cggtcaggat caacgcggcg gtcgtgacga ccgcggcgat
     1321 acgcgtcctc atgcttcgca aaccctccat cgctgctgtg cttggggcag tagcctgatg
     1381 ccgtgggagc aaccctccaa ggcattcggg cgcagtggtc gttgtggtcg ttctggtcgc
     1441 gcacgctcgc ccgccagctg ctgctgcttc agatcgccgt ggtcgccgtc accgtcgtcg
     1501 tcggcgcgct cgcgtcctac ggcctggtct ctaaccagat cgacgagcag tacaagcagc
     1561 gcgcactcgc gatcggatac gccgtcgccg cgacgccgga cgtgctcgac gcgatggacg
     1621 agcccgagcc cgcgcgcgtg atccagccca tcgccgaggc gatccgccgc tcggtcggcg
     1681 ccgacttcgt cgtcgtggcg aaccgcgacg gcatccgcta ctcgcatccg aacccggcgc
     1741 ggatcggcga gcgcgtctcc accgatcccg gtccggcgct cgcgggcaat cccttcgtcg
     1801 gcgtggagga gggcacgctc ggcagatcgc tgcgcgcgaa agtgccgatc acgaagaacg
     1861 gcgaggtgat cgggatcgtg tcggtcggct tcctcgagga gcaggtcgcg cagaagctcg
     1921 cgcaggcgct gccgacgatg acgctcacgg tcctgctggc gctcacgctc ggcgtcgcgg
     1981 ggtcgctcat cctcgcccgc cgcatcgagc gccagacctt cggcctcggc acgcgcgaga
     2041 tcgcgggact gctcgagcag cgcgacgcga tgctgcacgg gatccgcgag ggcgtgctcg
     2101 ccctcgacac gagcggacgc gtgaccgtcg cgaacgacga ggcgcgccgc ctgctcggca
     2161 tcaccggcac ggtcgagggc cgccggctcg ccgaggtcgt acccgaggga cgagcgcgcg
     2221 aggtgctcgg cggctccacg accggcaacg atcaggtggt gctggccggc gaccgcgtac
     2281 tcgtgctcaa ccggatgccg gtggccgtgc gcggccgcga ggtcggcgcg gtcgtgaccc
     2341 tgcgcgaccg cacggagctc gagggcgtcc tgcgcgagct cgatacggtg cgcggtctgg
     2401 cgcaggcgtt gcgcgcgcag acgcacgagt tctcgaacaa gctccatgtc gtcgccgggc
     2461 tcatcgagct cgggcgactc gaggaggccg tgcggttcat cgcgaagacg agcctcgtcc
     2521 accaggagct ggtcgagctg gtgcagcagc gcatcgccga ccccgcgctc gccgcgctgc
     2581 tgctcgccaa ggccgcgctc gcgtcggagc gccgcgtcga cctccgggtc gcgagcgacg
     2641 cccacctgcc gcgggacctc gccgacgtcc gcgatctcgt caccgtcgtc gg
//
LOCUS       DASTZR010000494         2024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1301885, whole genome shotgun sequence.
ACCESSION   DASTZR010000494 DASTZR010000000
VERSION     DASTZR010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301885"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VFV20_10950"
     CDS             complement(<1..383)
                     /locus_tag="VFV20_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018538932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEU5288911.1"
                     /translation="MTLERCLANLDEAIAAAVALGLDTTAAAAVRETGRTRLGFPSDT
                     YVLALAGGTGVGKSTMLNALAGEDVSPSSARRPTTADPVAWVPGGRGRELSGLLTWLG
                     VSRIREHAAGTLDDVAVLDLPDFDS"
     gene            complement(380..>2024)
                     /locus_tag="VFV20_10955"
     CDS             complement(380..>2024)
                     /locus_tag="VFV20_10955"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEU5288912.1"
                     /translation="DRLAALAERRLALAAASQPARERARQLVDHLQGFIRPRIADIDA
                     PLLVLLLGPTGAGKSSLLNTIAGAAVSPASALRPTTREAILYATEADAKSIRASGRLG
                     LVAIDRLSVVTAPDRTSGVAVVDAPDIDSVERDNRELADVLVEASDLCVFVTTATRYA
                     DLVPWQVLRRVRERGLPLVVVLNRLPADERDREIVIADARRLLAEFGLDVADERAELI
                     TVGEGDLDPRIDALAREKARPLLERIARLGSEAGERRAVATAALAGAIRGLSPLVHGV
                     ADDLEHEAIDADALRRIAETAYAEELEHLQRDLSSGVLLRGEVLRRWNDFVGADQVTR
                     FISSGLGRLRAMLLTAFRSAPNAPVAVVEEEMASTLEALALRSASDAARRTASAWSER
                     PTPAHLIAGDAALWSVSDRFAPALRGDLDVWMRATIDDVRVHAGRKRAVAQIAALGVN
                     AVGVAVMIGVFVHTAGLTGAEVGVAAGTAFLNQKLLEAVFGERAMEQLIGQARSRLDT
                     LLASLFMRERERFDALVPPPAELREVATALRDAVAGLRA"
BASE COUNT          277 a          750 c          727 g          270 t
ORIGIN      
        1 atcgagtcga agtcggggag gtcgagcacg gcgacgtcgt cgagcgtgcc cgccgcgtgc
       61 tcgcggatgc ggctcacccc gagccatgtg aggaggcccg aaagctcgcg gccgcgtccg
      121 ccgggcaccc atgccaccgg gtccgcggtc gtcggccgtc gcgcgctcga gggactcacg
      181 tcctcaccgg cgagcgcgtt gagcatcgtc gacttgccga cgccggtccc gcccgcgagc
      241 gcgagcacgt aggtgtcgct cgggaatccg agacgcgtac gcccggtctc gcggaccgcc
      301 gccgccgcgg tcgtgtccag gccgagcgcc acggccgcgg cgatcgcttc gtcgaggttg
      361 gcgagacaac gctcgagcgt catgcgcgca gacccgccac cgcatcgcgg agcgcggtgg
      421 ccacctcacg cagctccgcg ggcggcggga cgagcgcgtc gaaccgctcg cgctcgcgca
      481 tgaagagcga cgcgagcagc gtgtcgagcc tgctgcgtgc ctgaccgatg agctgctcca
      541 tggcccgttc gccgaagacg gcctcgagaa gcttctggtt caggaacgcc gtcccggcgg
      601 cgaccccgac ctcggcaccg gtgagtccgg cggtgtgcac gaacacgccg atcatcaccg
      661 ccacgccgac ggcgttgacg ccgagcgcgg cgatctgcgc cacggcgcgc ttgcggccgg
      721 cgtgcacgcg cacgtcgtcg atcgtcgcgc gcatccacac atcgagatcg ccgcggagcg
      781 cgggcgcgaa ccggtcggac accgaccaca gcgcggcgtc ccccgcgatc aggtgcgcgg
      841 gggtgggtcg ttccgaccat gcggacgcgg tccgccgtgc cgcatcgctc gcgctgcgca
      901 gcgcgagcgc ctcgagcgtg ctggccatct cctcctcgac gacggccacc ggtgcgttcg
      961 gcgcactccg aaaggccgtg aggagcatcg cccggagcct gcccagtccc gacgagatga
     1021 agcgcgtcac ctggtcggcg cccacgaagt cgttccagcg ccgcagcacc tctccgcgca
     1081 ggaggacgcc gctgcttagg tcccgctgga gatgttcgag ctcttccgca tatgccgtct
     1141 cggcgatgcg ccgcagcgcg tcggcgtcga tcgcttcatg ctccaggtcg tcggcgacgc
     1201 cgtgcacgag cggcgagagt cccctgatcg cgcccgcgag cgccgcggtc gcgacggcgc
     1261 gtcgctcgcc ggcctcggat cccaggcgcg cgatgcgctc gagcagcggc cgcgccttct
     1321 cgcgcgcaag agcgtcgatg cgcgggtcca ggtcgccctc gcccaccgtg atgagctccg
     1381 cgcgctcgtc cgcgacgtcg aggccgaact ccgccagcag ccggcgcgcg tcggcgatca
     1441 cgatctcgcg atcgcgttcg tcggccggga ggcgattgag cacgacgacg agtggcagac
     1501 cgcgctcccg cacgcggcgc agcacctgcc acggcacgag gtccgcgtaa cgggtcgcgg
     1561 tcgtgacgaa gacgcagaga tcgctcgcct cgacgagcac gtccgccagc tcgcgattgt
     1621 cccgctccac cgagtcgatg tccggtgcgt ccacgacagc gacgccgctc gtgcggtctg
     1681 gcgccgtgac gaccgacagg cgatcgatcg cgacgaggcc gagccggccg ctcgcccgta
     1741 tcgacttcgc atccgcctcg gtcgcgtaga ggatggcctc gcgcgtggtc gggcgcagcg
     1801 cgctcgccgg gctcaccgcg gcgccggcga tcgtgttcag cagcgacgat ttcccggctc
     1861 cggtggggcc gagcaggagc acgaggagcg gtgcgtcgat gtccgctatc cgcggccgga
     1921 tgaagccctg caggtgatcg acgagctgtc gcgcgcgctc gcgtgcgggc tggctcgcgg
     1981 cggccagtgc gagccgccgc tccgcaagag cggccaagcg gtcg
//
LOCUS       DASTZR010000495         5796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_75168, whole genome shotgun sequence.
ACCESSION   DASTZR010000495 DASTZR010000000
VERSION     DASTZR010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75168"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VFV20_10960"
     CDS             complement(<1..758)
                     /locus_tag="VFV20_10960"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEU5288913.1"
                     /translation="MTVSLKEDQLVLITEDDGSLPLGGSSPLGLYYHDTQFLSGYAMR
                     VNGTRPLLLSANTEQNYVATFQLMHSENMTVGTARHTADALSIRRTRFLTDGMRERIG
                     VLNANARPVRLSIDLIFEAAFKDMFAVRGNAEDTGAEVSVEPRADGLVFERVGRDAVR
                     RTTEITMRPAPDRVDGTTIHIERELAAQQVLTIELSVIPREDGAGEAPSPGRFDDALD
                     ALNARYRRFLRSCTRYTTSSETLDEGLITRSALDL"
     gene            complement(755..1963)
                     /locus_tag="VFV20_10965"
     CDS             complement(755..1963)
                     /locus_tag="VFV20_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEU5288914.1"
                     /translation="MPDLTALDDQLREGIARIGSADLLVGIPSFRNAGTIGHVATTVA
                     RGLREHFPGARVALVNADGGSEDGTPDRVADAADGVPVAVGRYVGPPGKGSAFRAIFQ
                     SAVALDARACAVVDSDLRSITPAWIQRLLGPVVAARADYVAPLYARHKHDGTITNSVA
                     YPLTRALYGHRIRQPIGGEFGFHSDLARTYLAQPVWDGDVARFGIDIFMTTTALARHA
                     KVVQAFLGAKVHDPKDPAADLGPMFTQVVGTLFHLAAENEPAWRGVTRSRGVPVEGDL
                     EPVEPDPIPADPARLRAKYEASAGEQRALWDEVLTAPLPRTMGPDEWARLVFDFLEAA
                     LRHPGESERLARGLLPLYYLRVAEFLDEVRDLDTVGSEDIIELGARAMEKEKADRLEA
                     VDESAHETTS"
     gene            2055..2690
                     /locus_tag="VFV20_10970"
     CDS             2055..2690
                     /locus_tag="VFV20_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEU5288915.1"
                     /translation="MLEAIDAIVLPFIDSLYATFGYLGVVIAMAIESAAIPIPSELIL
                     PFAGWSVSRGLTEPLTGTAWTYWGAVVAGVVGNTAGSLVSYAVGAVGGRPLVERYGRY
                     VLISAHDLELAERWFARYGEATVFFSRMLPIVRTFISVPAGVARMPLWRFLLFSILGA
                     IPWVMLLVWGGMVLGDHWLDLKHSLKGLDYLVAAAVLLLVGLFVWRHVKRR"
     gene            2691..5657
                     /locus_tag="VFV20_10975"
     CDS             2691..5657
                     /locus_tag="VFV20_10975"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU5288916.1"
                     /translation="MDAVLALDDQIYRAIFTATTSSAALSALAVGIYWLNWNGLLWWI
                     AAVVLARARGFGRRAWWAVLTVYLGIADGWITTELVLKPLVHRPRPFAAIQGLPPTLI
                     DHPASFAFPSGDATFAVGAAVALGSVAPRWRIPALLMAAAVCFERVAVGVHHPSDVIA
                     GALVGTVSGLTAPWIVANVRRRMRWRVFVVAHTHWDREWYERFEGYRARLVPMVSALL
                     DLLERDASFRSFTFDGQTIAIEDHLEKRPDDRERIAALVRAERLFVGPWYVLADNLLV
                     SGESVVRNFQEGLRVAQTFGRALRVAYVADPFGHPAQVPQILRGFGYRTYVFSRGVGD
                     EGEDLGAEFLWEAPSGDRVLASHLVDHYAGGLALVGELSETEAQLRARIRRRLARMVD
                     VPARYANSANLLFMEGDDHVVAYERLPEAVAVMHDVAPNLDVRIASLEEYEAAMSAPA
                     PVHAGEIVSGRYRPILRGVNATRVWIKQENAHCERLLLERCEPLDALSGGEDRDTIRS
                     LWRTLLQNHPHDSICGCSIDDVHDIDMAPRFLRVRTEGEALAATLERRVLGDGARQLV
                     WSDLAWPRTGVADLDGVPTAVDCHGLGAAEAVRAERGTVRSASDGVIENGALRVEVAA
                     DGSFFVTELETARRTGPHNVLLDEGDRGDEYTYSYAGPTVGSTGRSGTRRTEVRGDRA
                     TVIVEYTLELPAGLRADRLARAPETVGCPVRAEVSLDLGARRVDVRLTIDNRARDHRL
                     RVLCDTGTRSLTHVAGAAFAWLDRPTRIPAGSGWIERPTAERCGHDLVGIDGALVVGM
                     DGLRDYAPLHDGRTIAVTLLRAVGWLSRGDLPERKGHAGPALETPSAQCLGERTYRYC
                     VVPLGGEMTLALAGREVHEFLSPVRVIRAAAPRTLVSLDRSSPIQLSALRAASGGAVT
                     MRLFNPRGDQAETTVRFDRPVTHARPVDLREGDTGLGNTGLDVLRTAAPPEVADGALH
                     LALAPYEIGTYLVTLA"
BASE COUNT          747 a         2068 c         2071 g          910 t
ORIGIN      
        1 aggtcaagcg cgctgcgggt gatgagcccc tcgtcgagcg tctccgagct cgtggtgtac
       61 cgcgtgcacg atcgcaggaa ccgccggtaa cgcgcgttca gcgcgtcgag ggcgtcgtcg
      121 aaccggccgg gcgacggcgc ctcgccggcg ccgtcctcgc gcgggatcac cgacagctcg
      181 atggtgagga cctgctgcgc ggcgagctcg cgctcgatat ggatcgtggt gccgtccacg
      241 cggtcggggg cgggacgcat cgtgatctcg gtggtgcggc ggacggcatc gcgccccacg
      301 cgctcgaaga cgaggccgtc ggcgcgtggc tcgacgctca cctcggcgcc cgtgtcctcg
      361 gcgttgccgc ggaccgcgaa catgtccttg aacgcggcct cgaagatgag atcgatggag
      421 aggcgcacgg gtctcgcgtt cgcgttcagc acgccgatcc gttcgcgcat gccgtccgtg
      481 aggaagcgcg tgcggcggat cgagagcgcg tcggcggtat ggcgggcggt cccgacggtc
      541 atgttctcgg aatgcatgag ctggaacgtg gcgacgtagt tctgctcagt gttcgccgag
      601 aggaggagcg ggcgcgtccc gttgacgcgc atcgcgtacc cggaaaggaa ctgcgtgtcg
      661 tggtagtaga ggccgagcgg gctcgacccg ccgagcggca ggctcccgtc gtcctccgtg
      721 atcagcacga gctgatcctc tttgagggaa acggtcacga agtggtctcg tgcgcgctct
      781 cgtccacggc ttcgagacgg tccgccttct ccttctccat cgcacgcgct ccgagctcga
      841 tgatgtcctc ggagcccacg gtgtccaggt cgcgcacctc gtcgaggaat tcggccacgc
      901 gcaggtagta gagcggaagc aggccgcgtg cgagacgttc gctctcgccg ggatggcgga
      961 gcgccgcctc gaggaagtcg aagaccagtc gggcccactc gtcgggtccc atcgttcgcg
     1021 gcagcggcgc ggtgagcacc tcgtcccaca gggcacgctg ctcgccggcg ctcgcctcgt
     1081 acttcgcccg cagccgggcg ggatcggcgg ggatcggatc tggctcgacc ggttcgaggt
     1141 caccctccac gggcacgccg cgcgatcgcg tgacgccgcg ccaggccggc tcgttctcgg
     1201 cggcgagatg gaacagcgtg ccgacgacct gcgtgaacat cggtccgagg tccgccgcgg
     1261 ggtccttcgg atcgtggacc ttcgcgccga ggaaggcctg cacgaccttc gcgtggcgcg
     1321 cgagcgccgt cgtcgtcatg aagatgtcga tcccgaaccg ggcgacgtcc ccgtcccaca
     1381 ccggctgcgc gagataggtc cgcgcgaggt cggagtggaa gccgaactcg ccgccgatgg
     1441 gctgccggat gcgatggccg tagagcgcgc gtgtgagcgg gtaggccacc gagttggtga
     1501 tcgtcccgtc gtgcttgtgg cgcgcgtaca gcggcgcgac gtagtccgcg cgcgccgcaa
     1561 cgaccggccc gagcagacgc tggatccacg ccggagtgat gctgcgcaga tcggaatcga
     1621 ccaccgcgca cgcgcgcgcg tcgagcgcga ccgcggactg gaagatcgcg cggaaggccg
     1681 accccttgcc gggagggccg acgtagcggc cgaccgcgac ggggacccca tccgccgcgt
     1741 ccgcgacgcg gtcgggggtg ccgtcctcgg aaccgccgtc ggcgttcacc agggccaccc
     1801 tggccccggg gaagtgctcg cgcaggccgc gcgcgacggt cgtcgcgacg tggccgatcg
     1861 tccccgcgtt gcggaagctc gggatgccga cgagcagatc ggcgctgccg atccgtgcga
     1921 tgccttcgcg gagctggtcg tcgagggcag tcaagtcggg catgcgttct cctttgagca
     1981 gatcgtccct gaccgctagc gctgtcaggt ccggtcgaag atgccctgcc cgtcccttcg
     2041 gccatcatac gcaagtgctc gaagcgatcg acgcgatcgt cctgcccttc atcgactcgc
     2101 tgtacgcgac gttcggctac ctcggcgtgg tcatcgcgat ggccatcgag agcgccgcga
     2161 tcccgatccc atcggagctc atcctgccct tcgcgggatg gagcgtgtcg cgcgggctca
     2221 ccgagccgct caccggaacg gcgtggacat actggggcgc ggtggtcgcc ggcgtcgtgg
     2281 ggaacaccgc tggctcgctc gtgagctatg cggtcggggc agtcggtggt cgtccgctcg
     2341 tcgagcgcta cggacgctac gtgctcatca gcgcgcacga cctggagttg gccgaacgct
     2401 ggttcgcgcg ctacggcgag gccacggtgt tcttctcgcg catgctgccg atcgtccgca
     2461 ccttcatcag cgtgccggcc ggggtcgcgc gcatgcccct ctggcggttc ctgctcttct
     2521 cgatcctcgg cgcgatccca tgggtcatgc tcctcgtctg gggtgggatg gtcctcggcg
     2581 accactggct cgacctgaag cactcgctca aggggctcga ctacctcgtc gccgcggcgg
     2641 tcctcctgct cgtcggcctc ttcgtgtggc ggcacgtgaa gcgccgctag atggacgccg
     2701 tcctcgcgct cgacgaccag atctaccgcg cgatcttcac cgccaccacg agctccgccg
     2761 cgctctccgc gctcgccgtc ggcatctact ggctcaactg gaacgggctg ctctggtgga
     2821 tcgccgcggt cgtgctggcc cgcgcgcgcg ggttcggacg gcgcgcgtgg tgggcggtgc
     2881 tcaccgttta cctcgggatc gccgatggct ggatcacgac ggagctcgtg ctgaagccgc
     2941 tcgtgcaccg gccccgtccc ttcgcggcca tccagggatt gccgccgacg ctcatcgatc
     3001 atcccgcgag cttcgcgttc ccctcgggtg acgccacgtt cgcggtcggc gccgcggtcg
     3061 ccctcggcag cgtcgcgccg cgctggcgca tccccgcgct gctcatggcc gccgccgtct
     3121 gtttcgagcg cgtcgccgtt ggcgtgcacc acccgagcga cgtgatcgcc ggcgcgctcg
     3181 tggggacggt gtccggcctc accgcgccgt ggatcgtcgc gaacgtgcgc cgccgcatgc
     3241 gctggcgcgt gttcgtcgtc gcgcacacgc actgggaccg cgagtggtac gagcgcttcg
     3301 agggctatcg cgcgcgcctc gttcccatgg tctccgcgct cctggacctg ctcgagcgcg
     3361 acgcgtcgtt ccgctcgttc acgttcgacg gccagacgat cgcgatcgag gaccacctcg
     3421 agaagcgtcc ggacgaccga gagcgcatcg cggcgctcgt ccgtgccgag cgtctcttcg
     3481 tcggtccgtg gtacgtgctc gctgacaacc tgctggtctc gggcgagtcg gtcgtgcgga
     3541 actttcagga gggcctgcgg gtcgcgcaga ccttcgggcg cgcgctgcgc gtcgcgtacg
     3601 tcgccgatcc gttcggccat ccggcgcagg tcccgcagat cctgcgcggc ttcgggtatc
     3661 gcacctacgt cttctcgcgt ggcgtcggcg acgagggtga ggaccttggt gccgagttcc
     3721 tgtgggaggc gccctccggc gaccgcgtcc tggcatcgca tctggtcgac cactacgccg
     3781 gcgggctcgc gctcgtcggc gagctgtcgg agacggaggc gcagctccgg gcacggatcc
     3841 gccgacgcct ggcgcgcatg gtcgatgtcc cggcgcgcta cgcgaacagc gcgaatctgc
     3901 tgttcatgga gggggacgac cacgtcgtcg cgtacgagcg cctccccgaa gcggtggcgg
     3961 tcatgcatga cgtggcgccc aacctcgacg tgcgcatcgc gagcctcgag gagtacgagg
     4021 ccgcgatgtc ggcgccggcg cccgtccacg cgggcgagat cgtgagcggc cgctaccggc
     4081 cgatcctgcg cggcgtgaac gcgacgcgcg tctggatcaa gcaggagaac gcccattgcg
     4141 agcgcctgct gctcgagcgc tgcgagccgc tggacgcgct ctcgggcggg gaagaccgcg
     4201 acacgatccg ctcgctctgg cgcacgctgc tccagaacca tccgcacgac tcgatctgcg
     4261 ggtgctcgat cgacgacgtc cacgacatcg acatggcgcc ccgcttcctc cgcgtgcgca
     4321 ccgagggcga ggcgctggcc gcgacgctcg aacgacgcgt tctcggcgac ggcgcgcggc
     4381 agctcgtgtg gagcgacctg gcctggccgc gcaccggcgt cgccgatctc gacggcgtgc
     4441 ccaccgcggt cgactgccac ggcctcggcg cggccgaggc cgtccgcgcg gagcgtggga
     4501 cggtgcgctc cgcgtccgac ggcgtcatcg agaacggcgc gctgcgcgtc gaggtggccg
     4561 ccgacggcag cttcttcgtg accgagctcg agaccgcgcg gcgcaccggc ccgcacaacg
     4621 tcctgctcga cgagggtgac cgcggcgacg agtacaccta ttcctatgcg ggccccaccg
     4681 tcggttccac cggccggtcg gggacgcggc ggaccgaggt gcgcggtgat cgcgcgaccg
     4741 tgatcgtcga gtacacgctc gagctcccgg ccggcctgcg cgcggaccgc ctcgcgcgtg
     4801 cacccgagac ggtcgggtgc cccgtccgcg cggaggtctc gctcgacctg ggtgcccgac
     4861 gcgtcgacgt gcgcctcacg atcgacaacc gcgcgcgcga ccaccggctg cgcgtgctct
     4921 gcgacaccgg cacgcgttcg ctcacgcacg tcgcgggcgc cgcgttcgcc tggcttgatc
     4981 gtcccacgcg gatccccgcc gggagcggct ggatcgagcg gccgaccgcg gagcgctgcg
     5041 gtcacgacct cgtcgggatc gacggggcgc tcgtcgtcgg gatggacggt ctgcgcgact
     5101 acgcgccact ccacgatgga cgcaccatcg cggtgacgct gctccgcgcg gtcggctggc
     5161 tctcccgcgg cgacctgccg gagcggaagg gccatgccgg ccccgcgctc gagacgccga
     5221 gcgcgcagtg cctcggcgag cgcacgtatc gctactgcgt ggtgccgctc ggcggcgaga
     5281 tgacgctcgc gctcgccggc cgcgaggtgc acgagttcct ctcgcccgtc cgcgtgatac
     5341 gcgcggcggc gccgcgcacg ctggtcagcc tcgaccggtc gtcgccgatc cagctctcgg
     5401 cgttgcgcgc cgcgtcgggg ggcgcggtca ccatgcggct cttcaacccg cgcggcgacc
     5461 aggcggagac gaccgtgcgc ttcgaccgtc cggtgacgca cgcgcgcccc gtcgacctgc
     5521 gcgaaggcga cacgggcctc ggcaacaccg ggctcgacgt gctgcgcacc gccgcgccgc
     5581 ccgaggtcgc ggacggtgcg ctgcatctgg cgctcgcgcc gtacgagatc gggacctacc
     5641 tcgtcactct cgcgtgaaac gtgtgacgtg ccgtcgcacg aacgtgcgac gcgcgtggga
     5701 cagacgcgac cgcgacacct cgctagggtc ggcgcatgcg catcgccgtg gcactcctct
     5761 cgtgcgcgct ggcgctcacg caatccatcc cggcgt
//
LOCUS       DASTZR010000496         4303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_859744, whole genome shotgun sequence.
ACCESSION   DASTZR010000496 DASTZR010000000
VERSION     DASTZR010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859744"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VFV20_10980"
     CDS             complement(<1..498)
                     /locus_tag="VFV20_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288917.1"
                     /translation="MWTEDVPALKAVSVDAATPVFASETLEIDETLESAETEEMASPL
                     PLADAVAEEVARLRQEALAQLDSELLARRAELARSLERQRTESEQRIAEIERLETAAL
                     ARRREEIERSWRVDEGRVLAEQIDAAFATQLADLKDRYADAEARLRQHVETRQREEAA
                     RLEQWR"
     gene            complement(689..1771)
                     /locus_tag="VFV20_10985"
     CDS             complement(689..1771)
                     /locus_tag="VFV20_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288918.1"
                     /translation="MPASASLWAWARGHSRAIGFVMRLLPMLPSGPIDRVTRAPVIEN
                     VRYPSLSGEREGVVYRPPGPGPHPAIVLCLGVVPFGVDHPQVPRLETALARFGFLALI
                     HWSPAMRDLRLVPDDAEDIALAYEWLLSRADVDASRSGLFGTCVGGSFALLAAARPRI
                     RDRVAFVGAFAPFSSVSTLVRDIASGTTDDGVATSWAVDQLTRQVFVRSVTCMLEPAE
                     SDALRTAYEQGRPLDPPTLSVDARAVHALLFAGDRDAADDAMRGLPASLRERLDRMSP
                     LEHVAELAAPLVMFGHDRDDHVIPVGESRRLAAALAGRPGVRYTEYAMFEHADPTKRK
                     LSPLRLVVEVRKFYLSLYPMFRQAVG"
     gene            1913..2917
                     /locus_tag="VFV20_10990"
     CDS             1913..2917
                     /locus_tag="VFV20_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020134880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEU5288919.1"
                     /translation="MVAIAAAQTTEKMKALVQDGYGSADVLRIREVEKPVATEGRVVV
                     RVRAASLNALDWHSVHGGRVIQLASAVMRQKDEPVRGVDLAGVVESVGPNVTRFKPGD
                     EVFGGAPAAFAEYARAREAGLALKPRDLSFEEAATVGVAARTALQGLRDHAQLKPGQR
                     VLINGAGGGVGTFAVQIAKALGAHVTAVTGTRNVELIRSLGPDEVIDYTKEDFARRGE
                     RYDAIFDVAASRSFSDMRRVLVPGGIIVLAGADKRGGLAIFTRIGAAFLRSRLLKQRV
                     VFFVAKATLEDLLVLKGLLEERKIRPAIDRTYPLSEGAEAIRYLGTGNARAKVVITMG
                     "
     gene            3045..>4303
                     /locus_tag="VFV20_10995"
     CDS             3045..>4303
                     /locus_tag="VFV20_10995"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEU5288920.1"
                     /translation="MIPAIHDLILIHPPSVFDFRRHAQFRAPMAQVIPSTDQFEMYPM
                     GMTSIAAYLARNNYTVRIVNLARRMVGDPGFDAVRHLRGLDARVFGIDLHWLPHAHGA
                     LAVARLVKRLHPGAKVLMGGLSASYYRDELIRHPAVDFVITGDSTEEPCRQLLRALRE
                     RRPLESVANLTWKAGERVVHNPLSYVPTDIDRFDVPAYEYMLRSVAMHGRLADALPYE
                     GWLDQPLTVLLNARGCILDCAICGGSRSGYKLICGRARPAFRSPERLIEDLRVIRSFS
                     RSPIFVIHDPRIGGMPRARRFFELLARERLPNELVFELFAPARADFFEMVSKGARRWS
                     LQVTIETQEERLRAANGKFACTNERVEETIALAYEHGCRTLDLFFMVGVPGQTYDSAL
                     AIADYCERLLERFGTDGRLRPFVAPLAP"
BASE COUNT          647 a         1509 c         1496 g          651 t
ORIGIN      
        1 gcgccactgc tcgagacgcg cggcctcctc acgctgtcgc gtttccacgt gctgccgcag
       61 acgcgcttcg gcgtccgcat agcggtcctt gagatcggcg agctgcgtcg cgaacgccgc
      121 gtcgatctgc tccgcgagca cgcggccctc gtccacgcgc cacgatcgct cgatctcctc
      181 gcgacggcgc gcgagcgcgg ccgtctcgag acgctcgatc tcggcgatac gctgctccga
      241 ctcggtacgc tggcgctcga ggctgcgcgc gagctcggcc cggcgcgcca gcagctcact
      301 gtcgagctgc gcgagcgcct cctgacggag ccgcgccact tcctccgcga ccgcgtcggc
      361 gaggggtaat ggactggcca tctcctccgt ctcggcgctc tccagggtct cgtcgatctc
      421 cagggtctcc gaggcgaaca ccggcgtggc tgcgtcgacg ctgacagcct tcagcgcggg
      481 aacgtcctcg gtccacacga gctcggctcg tgcgctggtc tccgggcggc gccacacgct
      541 ggaagcgcgg atgagggacc ggggcgtctc tggctcgctc tggcctccat acgtcatgtt
      601 cagggtgtat ccgccggcgc gggaacggac catcacccgc caagggggag ggcgtcacac
      661 cgcagggtgt ttcgtgacgg tatctgcgct agccgacggc ctggcggaac atcggatata
      721 gcgagaggta gaacttccgc acctcgacca cgagccgcag gggcgagagc ttccgcttcg
      781 tcggatcggc gtgctcgaac atcgcgtact ccgtatagcg cacacccggc cgcccggcga
      841 gcgccgccgc gagcctgcgg gattcgccga ccgggatgac gtggtcgtcg cgatcgtggc
      901 cgaacatgac cagcggcgcc gcgagctcgg cgacgtgctc gagcggcgac atgcggtcga
      961 gccgctcacg gagcgacgcg gggagaccgc gcatcgcgtc gtcggcggcg tcgcggtcgc
     1021 ccgcgaacag gagcgcgtgc acggcgcgcg cgtccacgct cagcgtcggc ggatccaacg
     1081 gacgaccctg ctcatatgcc gtgcgcagtg cgtcggactc ggccggctcg agcatgcacg
     1141 tcaccgatcg aacgaagacc tgccgcgtga gctgatcgac cgcccacgac gtcgccacgc
     1201 cgtcatccgt cgtaccgctc gcgatgtcgc ggacgagcgt gctgaccgag gagaacggcg
     1261 cgaacgcgcc gacgaatgcc acccggtcgc ggatacgcgg acgcgcggcg gcgagcagcg
     1321 cgaaggaccc gccgacgcac gtcccgaaga gcccgctgcg cgaggcgtcg acgtccgctc
     1381 ggctcaggag ccactcgtag gcgagtgcga tgtcctcggc gtcgtcgggc acgaggcgga
     1441 ggtcgcgcat cgcgggcgac cagtggatca gcgcgaggaa gccaaagcgc gcgagcgccg
     1501 tctcgaggcg cgggacctgc gggtggtcga cgccgaacgg cacgacgccg aggcacagca
     1561 cgatcgccgg atgcggaccg ggacccgggg gccgatagac caccccctcg cgttcgccgg
     1621 agaggctcgg gtaccgcacg ttttcgatca ccggcgctcg cgtcacgcgg tcgatcggtc
     1681 cggacggcag catcggcagg aggcgcatca cgaagccgat cgcccgtgag tggccgcgtg
     1741 cccatgccca caggcttgcg ctcgccggca tcttggcacc cacgatcgag agcgtgacgg
     1801 tcgattgcgt cagcccgctg tcttgcccta ctcgcccagc agcattcaca tttcctccac
     1861 atccgcccat cactgtcccc tcagcggatc gacgacaagg agggtcaaag tggtggtagc
     1921 gatcgcagcg gcacagacca cggagaagat gaaggcgctc gtccaggatg ggtacggctc
     1981 ggcggacgtg ctgcggatcc gggaggtcga gaagccggtc gccacggagg gccgcgtcgt
     2041 cgtgcgggtc cgcgcggcct ccctgaacgc gctggactgg cactcggtgc acggcggacg
     2101 cgtgatccag ctggcgtcgg ccgtgatgcg gcagaaggac gagccggtcc gcggcgtgga
     2161 cctcgcgggc gtcgtggaat cggtaggccc gaacgtcacg cgcttcaagc cgggcgatga
     2221 ggtcttcggc ggcgcgcccg cggccttcgc cgagtacgcg cgcgcgcgcg aggcgggcct
     2281 cgcgctcaag ccgcgcgacc tgtccttcga ggaggccgcg acggtcggcg tcgcggcgcg
     2341 caccgcgctc cagggcctgc gcgaccacgc gcagctgaag cccggacagc gcgttctgat
     2401 caacggagcg ggcggcggcg tggggacgtt cgccgtgcag atcgcgaagg cgctcggagc
     2461 gcacgtgacg gcggtcacgg gcacgcggaa cgtcgagctg atccgttccc tcggaccgga
     2521 cgaggtcatc gactacacga aggaggactt cgcgcgacgc ggtgagcgct acgacgcgat
     2581 cttcgacgtc gcagcgagtc gctcgttctc ggacatgcgg cgcgtgctcg ttcccggcgg
     2641 catcatcgtg ctggcgggag ccgacaagcg cggcggtctc gccatcttca cgcggatcgg
     2701 cgcggccttc ttgcgctcgc gcctgttgaa gcagcgggtc gtcttcttcg tcgcgaaggc
     2761 gactctcgaa gacctgcttg tcctgaaggg gctcctcgaa gagcgaaaga tccgcccggc
     2821 gatcgaccgc acctacccgc tgagcgaggg cgccgaagcg atccgctacc tcggcaccgg
     2881 aaacgccagg gcgaaggtcg tcatcacgat gggctgacgc gtcgctcgcg attgagggtc
     2941 ggctgaggat cctgggcccg gcggtggtcc cgggcgggga cgttagggtc caaagactcg
     3001 caggcatgcc ctgccggcac gcgcgtcagc ccagaccggc tgggatgatc ccagccatac
     3061 acgacctcat cctcatccat ccgccgtcgg tgttcgactt ccgccggcac gcgcagttcc
     3121 gcgcgccgat ggcgcaggtc atcccctcga ccgaccagtt cgagatgtat ccgatgggga
     3181 tgacgagcat cgccgcctac ctcgcgcgga acaactacac ggtcaggatc gtgaacctcg
     3241 cgcggcgcat ggtcggtgat cccggcttcg acgccgtgcg gcacctgcgc gggctcgacg
     3301 cgcgtgtctt cggcatcgat ctccactggc tgccgcacgc gcatggtgcg ctggccgtgg
     3361 cgcgcctcgt gaagcggctg catcccggcg cgaaggtgct gatgggcggc ctgtccgcga
     3421 gctactaccg ggacgagctc atccgccacc cggcggtgga cttcgtcatc accggcgatt
     3481 cgacggagga gccgtgccgg cagctgctgc gagcgctgcg cgagcgccgg ccgctcgagt
     3541 cagtggcgaa tctcacgtgg aaggccggcg agcgcgtcgt tcacaacccg cttagctacg
     3601 tccccaccga catcgaccgg ttcgacgtcc ccgcgtacga gtacatgctg cgctccgtcg
     3661 cgatgcacgg tcggctcgcc gacgcgctgc cctacgaggg atggctcgat caacctctca
     3721 cggtgctgct caatgcgcgc ggctgcatcc tcgactgcgc catctgcggc ggctcgcgat
     3781 cgggctacaa gctgatctgc gggcgtgcgc gacccgcgtt ccgttcaccc gagcggctca
     3841 tcgaggatct ccgcgtcatc cgcagcttct cgcggtcgcc catcttcgtc atccacgacc
     3901 cacgtatcgg cggcatgccg agggcgcgcc gcttcttcga gctcctcgcg cgcgagcggt
     3961 tgcccaacga gctcgtcttc gagctgttcg ccccagcgcg ggccgacttc ttcgagatgg
     4021 tctcgaaggg cgcgcgtcgc tggagtctgc aggtcacgat cgagacacag gaggagcgtc
     4081 ttcgcgccgc gaacgggaag ttcgcctgca cgaacgagcg ggtcgaggag accatcgcgc
     4141 tggcgtacga gcacggctgc cgcacgctcg acctcttctt catggtcggc gtgccaggac
     4201 agacgtacga ctcggcgctc gcgatcgcgg actactgcga acggctgctc gagcgcttcg
     4261 gcaccgacgg tcgcctgcgt cccttcgtcg cgccgctcgc ccc
//
LOCUS       DASTZR010000497         6348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_388599, whole genome shotgun sequence.
ACCESSION   DASTZR010000497 DASTZR010000000
VERSION     DASTZR010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6348
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388599"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VFV20_11000"
     CDS             <1..396
                     /locus_tag="VFV20_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288921.1"
                     /translation="DLLAQWDRIKREEREAGGNADESILSGVPKGLPALFAAERLQER
                     SARVGVTHEPIDLPLDIDDPAFLGDLLFDLVAAAREKGFDAETALRQANERFAAHVAR
                     IEERARREGRALESYAPEEMRRMWEESLA"
     gene            393..1103
                     /gene="rph"
                     /locus_tag="VFV20_11005"
     CDS             393..1103
                     /gene="rph"
                     /locus_tag="VFV20_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEU5288922.1"
                     /translation="MTKPFKRPDGRAAEALRPITIEIGVSKFAEGSARISFGDTQVLC
                     LATIEESVPRHLIGKEQGWVTAEYAMLPRSTPERSRRESLEGRPGGRTFEIQRLVGRA
                     LRSAVDLKALGSRSVIVDCEVLQADGGTRTAAITGGYVALAHALRHFTPRPVRKQVAA
                     VSAGFVQGELMLDLAYAEDSIADADVNMVATSDGELIEVQGTAEGRPFSKEDFDRVLD
                     LGLRGIAKLVEAQRAALE"
     gene            1104..1658
                     /locus_tag="VFV20_11010"
     CDS             1104..1658
                     /locus_tag="VFV20_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEU5288923.1"
                     /translation="MPAANYPRIHLKTTAEWRRWLRDNHATAPGVWLVSWKRSAKKPF
                     VEYEDAVQEALCYGWIDGQLRPIDDERSSLLFTPRRAGSAWARSNKERVKKLIAERRM
                     RPAGLAKIEAAKRDGSWTLLDSVEALEVPADLRRALAAAGATRAFAALSKSAKRAHLY
                     ALVTAKKPETRAKRLADIVRSLGA"
     gene            1655..2263
                     /gene="rdgB"
                     /locus_tag="VFV20_11015"
     CDS             1655..2263
                     /gene="rdgB"
                     /locus_tag="VFV20_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEU5288924.1"
                     /translation="MSLHGEHVLLIGSGNPGKQKEWRELLSGIDAVLVTPQDLDPVPA
                     EPEEEGADFAENAAHKARAYAVATGLRTVADDSGLEVDALRGAPGLRSRRFFGDDASP
                     EERNTRLLALMEGVAARGARFVCVAALASPDGRVELFEGEVRGEIGHAPRGAQGFGYD
                     PIFIIAGDGRTMAELGPDEKHQISHRGLAAAQLRARLSEEPR"
     gene            complement(2276..2500)
                     /locus_tag="VFV20_11020"
     CDS             complement(2276..2500)
                     /locus_tag="VFV20_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288925.1"
                     /translation="MPRKKRSGFGDLVIARDAFERGEGRCPQCRLPIGPADQPTFVKS
                     FGTAPPTLATMRCGRCNAMLTLRFEETASS"
     gene            complement(2531..3634)
                     /locus_tag="VFV20_11025"
     CDS             complement(2531..3634)
                     /locus_tag="VFV20_11025"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5288926.1"
                     /translation="MIAFPPDLDRAVKTLGWVQLTPIQDRVIPIIRSGRDVIGQAQTG
                     TGKTGAFALPLLERVSGPARRPFVLVVVPTRELCLQVAKDFAALGRYRGVRVAAVYGG
                     NGYGPQERELRAGVHVVVGTPGRLLDLVERRTIDLGGVKVLVLDEADRLLDLGFINDV
                     RRIAGLVPSGRQTALFSATFPLAVDKVARSLTVDATRVSVKPEQPTIDTVDQSYIEVR
                     HDDKLRALREVLGRDEASRVLVFRRTKRGADKLARDLVRLGHDAAALHGDIPQRARER
                     VLEGFRRGTVSTLIATNLAARGLHVEGIDHVVNFDLPEDPETYVHRIGRTGRAGERGK
                     ALTFVSEGQHAEFQELKRKARVPFRQERLALYS"
     gene            complement(3743..4621)
                     /locus_tag="VFV20_11030"
     CDS             complement(3743..4621)
                     /locus_tag="VFV20_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU5288927.1"
                     /translation="MTTRGWLLFAAMSVIWGIPYLLIKVAVAEVEPSVLVLGRTVIGA
                     VLLVPIALVRSNVRALRPHWRLLVVYTFVEIVGPWLFLSHAETRLSSSLSGLLIAGVP
                     LVGAVLAWSTSHEDRPDAWRVGGLLLGLFGVALVVGFNVAADDLVAVGEVGLVVLGYA
                     IGAMLIARMREVPSIAVVAATLALAALVYLPFGIAQRPVALPSPQALGAIAILGVICT
                     ALAFIVFFALIHEVGPNRATVVTYVNPAVAVALGVWLLGEPLTPTIVAGFVLIALGSF
                     VGTRRSAALAREPAVV"
     gene            complement(4712..5248)
                     /locus_tag="VFV20_11035"
     CDS             complement(4712..5248)
                     /locus_tag="VFV20_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEU5288928.1"
                     /translation="MRIWTIGHGTRSIDEFLAILREARVVTLVDVRTFPGSRRHPQFG
                     KDALRASLGDAGIAYMHLPGLGGRRDPRPDSPHIALRVEAFRGYADHMGTPEFQTDLA
                     RLVAVAAATTTAYMCAETLWWRCHRRMLSDLLVAGDWEVTHLLGPGKSEPHRLWDVAR
                     VADGVLVYDGGAIPLEPQ"
     gene            5275..5850
                     /locus_tag="VFV20_11040"
     CDS             5275..5850
                     /locus_tag="VFV20_11040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5288929.1"
                     /translation="MSDVTIRAISPDQHDAVMHYFDMVAYADNPNWSTCFCCERLCTD
                     YSERTKEQNRELRSELIRSAKANGLVAYRLGRVVGWCHAAPKSELVNVPGPVAPDLGA
                     IVCFVVAPDQRRQGIATALLDAALAHLAKRGMRTVEAYPLIGEIEGARWVYQNYVGPL
                     TMYVKAGFQVTERLDGQCIVRRSLEPALVAR"
     gene            5888..>6348
                     /locus_tag="VFV20_11045"
     CDS             5888..>6348
                     /locus_tag="VFV20_11045"
                     /inference="COORDINATES: protein motif:HMM:NF015441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEU5288930.1"
                     /translation="MTGTVAWLHVAPIKSLQIQTRERVTLTSRGVEEDRRFCIVDDDG
                     RMLNAKRVGAFISVRPEFDDEMRELTLHLPDGRTICGAVELGEPITVSIYRRDVAARV
                     VRGQFTEALSSLDGRSARLVRFDEPGEGVDRAEKSGHVSLLSAASLDVLAEA"
BASE COUNT         1013 a         2182 c         2303 g          850 t
ORIGIN      
        1 gatctgctcg cgcagtggga ccgcatcaag cgcgaggagc gcgaggccgg tggcaacgcc
       61 gacgagagca tcctgtccgg cgtcccaaag ggccttcccg cgctcttcgc ggcggagcgg
      121 ctccaggaac gcagcgcgcg cgtcggcgtc acgcacgagc cgatcgacct gccgctggac
      181 atcgacgatc ccgccttcct cggagacctc ctcttcgatc tcgtcgcggc ggcgagggag
      241 aagggattcg acgccgagac ggcgctgcgc caggccaacg aacgcttcgc cgcgcacgtc
      301 gcgcgcatcg aggagcgcgc gcggcgagag ggccgcgcgc tggagtcgta cgctcccgag
      361 gagatgcggc ggatgtggga ggagagcctc gcatgacgaa gcccttcaag cggcccgacg
      421 gacgcgcggc ggaggcgctg cggccgatca ccatcgagat cggcgtctcg aagttcgcgg
      481 agggctcggc gcgcatctcg ttcggcgaca cgcaggtgct gtgtctcgcg acgatcgagg
      541 agagcgtgcc gcggcacctg atcggcaagg agcagggctg ggtcaccgcc gagtacgcga
      601 tgctgccgcg ctcgacgccc gagcgcagcc ggcgcgagag cctcgaggga cgccccggtg
      661 gccgcacctt cgagatccag cggctcgtcg ggcgcgcgct ccgctccgcg gtcgacctga
      721 aggcgctcgg ctcgcggagc gtgatcgtcg actgcgaggt cctgcaggcc gacggcggga
      781 cgcgcaccgc cgcgatcacc ggcggctacg tcgcgctcgc gcacgcgcta cgccacttca
      841 cgccgcgtcc ggtacgcaaa caggtcgcgg cggtctccgc cggcttcgtg cagggcgagc
      901 tgatgctcga cctcgcctac gcggaggact cgatcgccga cgccgacgtg aacatggtcg
      961 cgacgagcga tggcgagctc atcgaggtgc agggcaccgc tgagggccgg ccgttctcga
     1021 aggaggactt cgaccgcgtc ctcgacctcg gcctgcgcgg catcgcgaag ctcgtcgagg
     1081 cacagcgcgc cgcgctcgag taaatgcccg ccgccaacta cccgcgcatc cacctgaaga
     1141 cgaccgcgga gtggcgccgc tggctgcgcg acaaccatgc gacggcgccg ggcgtctggc
     1201 tcgtgtcgtg gaagcgcagc gcgaagaagc cgttcgtcga atacgaggat gcggtgcagg
     1261 aggcgctctg ctacggctgg atcgacggac agctgcggcc gatcgacgac gagcgctcga
     1321 gcctcctctt cacgccgcgc cgtgccggta gcgcgtgggc gcgctcgaac aaggagcgcg
     1381 tcaagaagct catcgccgag aggcgcatgc ggcccgcggg tctcgccaag atcgaggccg
     1441 ccaagaggga cggatcgtgg accctcctcg acagcgtcga ggccctcgag gtccccgcgg
     1501 acctgaggag ggcgctcgcg gcagcgggtg cgacgagagc ctttgccgcg ctctcgaagt
     1561 ccgcgaagcg cgcgcacctc tacgcgctgg tcacggcgaa gaagccggag acacgcgcga
     1621 agcggctggc cgacatcgtg cggagcctcg gcgcgtgagc cttcacggcg agcatgtgct
     1681 cctcatcgga tcggggaatc caggaaagca gaaggagtgg cgcgagctcc tgtcggggat
     1741 cgacgccgtg ctcgtgacgc cgcaggacct cgatccggtc ccggccgagc ccgaggagga
     1801 aggcgccgac ttcgcggaga acgcggcgca caaggcgcgc gcgtatgcgg tggcgaccgg
     1861 cctgcgcacg gtcgcggacg actcggggct cgaggtagac gcgctccgcg gcgcgcccgg
     1921 tctgcgcagc cggcgcttct tcggcgacga cgccagtccg gaggagcgga acacgcgcct
     1981 cctcgcgctc atggagggcg tcgcggcgcg cggcgcccgc ttcgtgtgcg tggcggcgct
     2041 ggcctcgccg gacggccggg tggagctctt cgagggtgaa gtgcgcggcg agatcggcca
     2101 cgcgccgcgc ggcgcgcagg gcttcggcta cgacccgatc ttcatcatcg ccggcgacgg
     2161 gcgcaccatg gccgagctgg gtcccgacga gaagcaccag atctcgcatc gcggcctcgc
     2221 ggcggcgcag ctgcgcgcgc ggctgtccga ggagccgcgc tagctgcgag gcgacctagc
     2281 tggaggcggt ctcctcgaag cgaagcgtca gcatcgcgtt gcaccgcccg caccgcatcg
     2341 tcgcgagcgt gggcggcgcc gtgccgaagc tcttcacgaa cgtgggctgg tcggcgggac
     2401 cgatgggcag acggcactgc gggcagcggc cctcgccccg ctcgaacgcg tcgcgggcga
     2461 tgacgaggtc gccgaatccg ctgcgctttt tccttggcac tggctgcctc ccccggtcac
     2521 gctaccgctg ttacgagtaa agcgcgagcc gctcctggcg gaatggcacg cgggccttgc
     2581 gcttcagctc ctggaactcg gcgtgctggc cctcgctgac gaacgtcagc gccttgccgc
     2641 gctcgcccgc ccggccggtg cggccgatgc ggtggacgta cgtctccggg tcctcgggca
     2701 ggtcgaagtt cacgacgtgg tcgatgccct ccacgtggag accgcgtgcc gcgaggttcg
     2761 tcgcgatgag ggtcgagacc gtgccgcggc ggaagccctc gagcacgcgc tcgcgggcac
     2821 gctgcgggat gtcgccgtgc agcgccgcgg cgtcgtggcc gagacggacg agatcgcgcg
     2881 ccagcttgtc agcgccgcgc ttggtgcggc ggaagacgag cacgcggctc gcctcatcgc
     2941 gccccagcac ctcgcggagc gcgcgcagct tgtcgtcgtg ccggacctcg atgtagctct
     3001 gatcgacggt gtcgatggtc ggctgctccg gcttcaccga gacgcgcgtc gcgtccacgg
     3061 tcagcgagcg cgcgaccttg tccacggcga gcgggaacgt cgccgagaag agcgcggtct
     3121 ggcggcctga cggcacgaga cccgcgatcc tgcggacgtc gttgatgaac cccaggtcga
     3181 ggaggcggtc cgcctcgtcg agcacgagca ccttcacgcc gccgaggtcg atcgtgcggc
     3241 gctcgacaag gtcgagcagc cggccgggcg taccgacgac gacgtggaca ccggcgcgga
     3301 gctcacgctc ctgcgggccg tagccgttgc cgccgtacac ggccgcgacg cgtacgccgc
     3361 gatagcggcc gagcgcggcg aagtccttgg cgacctggag gcagagctcg cgcgtcggta
     3421 cgacgacgag cacgaatggc cggcgcgcgg gtccgctgac gcgctcgagc agcggcagcg
     3481 cgaaggcgcc ggtctttccg gtgccggtct gggcctgacc gatgacgtcg cggcccgatc
     3541 ggatgatcgg gatgacacgg tcctggatgg gtgtgagctg gacccagccg agcgtcttta
     3601 cggctcggtc aaggtcgggt gggaatgcga tcacgcggtg taattccttc tcgggtggcg
     3661 aatcaacaaa aaaggcccgg aagcactcgc ttccaggcca ccttctcgta gtttgacccc
     3721 gaatgtttgc ttgtctctca gtctatacga cagcaggctc cctggcgagc gccgccgagc
     3781 ggcgcgtccc gacgaacgag ccgagcgcga tgaggacgaa tccagcgacg atcgtcggcg
     3841 tcagaggctc gccgagaagc cagacgccga gcgcgaccgc caccgccggg ttcacgtacg
     3901 tcacgaccgt cgcgcggttc ggaccgacct cgtggatgag cgcgaagaag acgatgaacg
     3961 ccagcgcagt gcagatgacg ccgaggatcg cgatcgcgcc gagcgcctgc ggcgacggca
     4021 gcgccactgg acgctgcgcg atgccgaacg ggaggtacac gagcgccgcg agcgcgagcg
     4081 tcgcggccac gacggcgata gacgggacct cgcgcatgcg tgcgatgagc atcgccccga
     4141 tggcatagcc gagcacgacg aggccgacct cgccgacggc gacgaggtca tcggcggcga
     4201 cgttgaatcc cacgacgagc gcgacgccga acaacccgag cagcaggccg ccgacccgcc
     4261 atgcgtccgg ccggtcctca tggctcgtcg accacgcgag caccgcgccg acgagcggca
     4321 cgccagcgat gagcaggccc gagagcgagc tcgaaagtcg cgtctcggcg tgcgagagga
     4381 agagccacgg gccgacgatc tccacgaacg tgtacacgac gagcagacgc cagtgcggcc
     4441 gaagcgcgcg cacgttcgag cgcacgagcg cgatcgggac gagcagcacc gcgccgatga
     4501 cggtgcgtcc gaggacgagc accgacggct cgacctccgc gacggcgacc ttgatgagca
     4561 gataggggat gccccagatc acgctcatcg ccgcgaacag cagccagcca cgtgtggtca
     4621 tgtgcccacc gacgctacaa cgcgcatgcg gcccgtgccg tgagacgtcg cgcgcgtgac
     4681 accgcgagat cgcggcgatg ctctcccgtc ctcactgagg ctcaagcggt atcgcgccgc
     4741 cgtcgtacac gaggacgcca tcggcgacac gcgccacgtc ccagaggcga tgcggctcgc
     4801 tcttgccggg gccgagcagg tgcgtcacct cccagtcgcc ggccacaagg aggtccgaga
     4861 gcatgcgccg gtgacagcgc caccacagcg tctcggcgca catgtacgcg gtcgtcgtcg
     4921 cggcggcgac cgccacaagg cgcgcgagat cggtctggaa ctccggtgtg cccatgtggt
     4981 cggcataccc gcggaaggcc tcgacgcgca gcgcgatgtg cggcgaatcg ggccgcgggt
     5041 cgcgacggcc gccgaggccg ggcaggtgca tgtacgcgat accggcgtca ccgagcgacg
     5101 cgcggagcgc gtccttcccg aactgcgggt ggcggcgcga cccggggaac gtgcgcacgt
     5161 ccacgagcgt gacgacgcgg gcttctctca ggatggcgag gaactcatcg atcgaccgcg
     5221 tgccgtggcc gatcgtccag atgcgcatct cctgagtgtg gaacactcgg gtctgtgagc
     5281 gacgtgacca tccgggcgat ctcgccggat cagcacgacg cggtcatgca ctacttcgac
     5341 atggtcgcgt acgccgacaa tccgaactgg tccacgtgct tctgctgcga acgtctctgc
     5401 accgactact cggagcgcac caaggagcag aaccgcgagc tccggagcga gctgatccgc
     5461 tccgccaagg cgaacggcct cgtcgcgtac cgtctcggcc gcgtcgtcgg ctggtgccac
     5521 gcggctccca agagcgagct cgtgaacgtg ccgggtccgg tcgcacccga tctcggcgcg
     5581 atcgtctgct tcgtcgtcgc gccggaccag cggcggcagg gcatcgcgac ggcgctgctc
     5641 gacgcggcgc tggcgcatct cgcgaagcgc gggatgcgga cggtcgaggc gtacccgctc
     5701 atcggagaga tcgagggggc gcggtgggtc taccagaact acgtcggccc gctcacgatg
     5761 tacgtgaagg cggggttcca ggtcacggag cgcctcgacg gccagtgcat cgtgcggcgg
     5821 tcgctcgagc cggcactcgt cgcgcgctga tccggcgaac gccgaaggcc gcagcgacaa
     5881 tggacgcatg accgggaccg tcgcgtggct gcacgtcgcg ccgatcaagt cgctccagat
     5941 ccagacgcgc gagcgcgtca ccctcacctc ccgtggcgtc gaagaggacc ggcgcttctg
     6001 catcgtcgat gacgatgggc ggatgctgaa cgccaagcgc gtcggtgcgt tcatcagcgt
     6061 gcgcccggag ttcgacgacg agatgcgcga gctgacgctg cacctgcccg acggcaggac
     6121 gatctgcggc gcggtggagc tcggcgagcc catcaccgtc tcgatctacc ggcgcgacgt
     6181 ggcggcgcgt gtggtccgcg gtcagttcac cgaggcgctc tcgtcgctcg acggacggag
     6241 cgcacggctc gtccgtttcg acgagccggg cgagggcgtc gaccgcgccg agaagagcgg
     6301 ccacgtgagc ctcctctcgg ccgcgtcgct cgacgtgctc gccgaggc
//
LOCUS       DASTZR010000498         3804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_859979, whole genome shotgun sequence.
ACCESSION   DASTZR010000498 DASTZR010000000
VERSION     DASTZR010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859979"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VFV20_11050"
     CDS             complement(<1..244)
                     /locus_tag="VFV20_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288931.1"
                     /translation="MVLGGAVVVKNVVSPETSSTTQTNTTGPIPATMRRVSLNIDGAT
                     HDLVVDNRESLWETMNFQLGLANSNLGCDRAQCGACA"
     gene            647..1762
                     /locus_tag="VFV20_11055"
     CDS             647..1762
                     /locus_tag="VFV20_11055"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HEU5288932.1"
                     /translation="MREVYARMAELEREGRRFAVCTVVRTVGSTPQVVGAKLLVDDLG
                     RLCGTLGGGCVEGDAFVEAKRVIATGDPSLREYELTEDLAWDTGLVCGGTMWISIEPG
                     DRALRLAGRDLLGDVLAASSGGRPVALATLLEKKDRDLAPQSKLFVETDGAAHGTLGD
                     ATLDARALTAAEEALRQGTARTITLDDRHELLIEPVVAKPHLVIAGGGHVGLALAKMA
                     AMLEYEVTVIDDREEFASRERFGPGIDVVRMDMMEALRSLPMGWNSFIVIATRGHKLD
                     SHCLREAVKRDVRYVGLLGSKRKTVLIAKMLRDEGVPEERIRRVHAPIGLDLGGRTPG
                     EIALSVLAEISLERFGGTGRALRLPEEIHDRTVSGRR"
     gene            complement(2006..2212)
                     /locus_tag="VFV20_11060"
     CDS             complement(2006..2212)
                     /locus_tag="VFV20_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288933.1"
                     /translation="MRKENEERPGPQGDEGDPDKGRRMDDERASEREEGITAARRGSR
                     AAPSDRPENEPPHPQDVNEIGESR"
     gene            complement(2250..3236)
                     /locus_tag="VFV20_11065"
     CDS             complement(2250..3236)
                     /locus_tag="VFV20_11065"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5288934.1"
                     /translation="MPRWDLHTHYYPQSYFRLIEEVGGDFSFGQSPTGQTIIRFRGSR
                     FFGITPPMTDAARRLEDMDRVGIDVEVLSLSTPNVYFAPPERQAEVARLANDAYAELI
                     AKHPSRFKGFASIPMDDPDAALRELDRALGELRLCGVVVLSNVNGRALTDARYRPFFE
                     ECDRRSACVFIHPMIPANAEAFAEYVLGPIVGFPFDTTLAVARLCYAGVFKQLPRIRW
                     ILGHLGGAVPYLFERLDNGWRDFAECRANIDELPSVYLRRLYYDTVSFSAPSLRLAIA
                     QVGADHLVMGSDYPHLLGSIERSVSSIEALDIPQADKDRIASGNARTILQNL"
     gene            complement(3253..>3804)
                     /locus_tag="VFV20_11070"
     CDS             complement(3253..>3804)
                     /locus_tag="VFV20_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288935.1"
                     /translation="GAAKLAQYKVTYKITATGAGAEGFSGDQTWYFKPPRSRFDFAMT
                     AGGQKSTISFFTLPEGTFYCMTAGGQATCFSVGGVGSPLDQNMAAVTQRSLIEHPESY
                     GATAAPSRTIAGQQGLCYAVTPVASAATGFTSGTFCYSKDGIALLSQFSAQGSSWSME
                     ATNLSTTVPDSDFTLPAKPTTLP"
BASE COUNT          591 a         1283 c         1339 g          591 t
ORIGIN      
        1 ccgcgcacgc gccgcactgc gcgcgatcgc agccgagatt gctgttcgcg aggccgagct
       61 ggaagttcat cgtctcccag aggctctcgc ggttgtcgac gacgagatcg tgcgtggcgc
      121 catcgatgtt cagcgacacg cgccgcatcg tcgcggggat cggaccagtg gtgttggtct
      181 gtgtcgtgct cgatgtctcg ggactgacga cgttcttgac gacgaccgcg cccccgagga
      241 ccacacccgc tgtcgctgcg ccggcacccg cgccaatgag gaaatcacgc cgcgtgactt
      301 ggagaccgtt accctttgat tgcgtctgtg ccgtgtctgt cactgcctgg tcaggaccgc
      361 ctcccgtgtc acccatagac gaaaccccca agcccatcgg tctggtcgat ggctggcgag
      421 agactagcac cggcgtacta gaaattgggc aagctggacc ggtacgccgt ctggtctgac
      481 cacctgagaa ttagtccgat gccgggtgtt ccgctggtcg caaacgcgac gcgccacgga
      541 cgaccgcggg gcgctctcat gaatcccgct gatccaccgg ccctcgtcgt ggaccgcgac
      601 gaacaaggca ccggatgccc gggcccgcgc cctatcctcg atggcgatgc gcgaggtcta
      661 tgcccggatg gccgagctcg agcgcgaagg acgccgtttc gcggtgtgca ccgtcgtccg
      721 gaccgtaggc agcacgccgc aggtggtcgg cgccaagctc ctggtcgacg acctggggcg
      781 tctctgcggc accctcggag gcggctgcgt cgagggcgac gcgttcgtcg aggcaaagcg
      841 cgtcatcgcg accggcgatc cgtcgctgcg cgagtacgag ctcaccgagg atctcgcgtg
      901 ggacaccggg ctcgtctgcg gcggcacgat gtggatctcc atcgagcccg gtgaccgcgc
      961 cctccgtctc gccggacgcg atctgctggg cgacgtcctc gcggcatcgt ccggcggccg
     1021 accggtcgcg ctcgcgacgc tgctcgagaa gaaggaccgc gacctcgccc cgcagagcaa
     1081 gctcttcgtc gagaccgacg gcgccgcaca cggcacgctc ggcgacgcga cgctcgacgc
     1141 gcgagcgctg acggcggccg aagaggcgct gcgtcagggc accgcgcgca ccatcacgct
     1201 cgacgatcgc cacgagctcc tcatcgagcc cgtcgtcgcg aagccgcacc tcgtgatcgc
     1261 cggcggcggc cacgtcggcc tcgcgctcgc gaagatggcc gcgatgctcg agtacgaggt
     1321 caccgtgatc gacgaccgtg aggagttcgc gtcgcgcgag cggttcggcc ctggcatcga
     1381 cgtcgtgcgc atggacatga tggaggcgct gcgctccctt ccgatgggct ggaacagctt
     1441 catcgtcatc gcgacgcgcg gccacaagct cgactcgcac tgcctgcgcg aggccgtgaa
     1501 gcgcgacgtc cggtacgtcg gcctgcttgg gagcaaacgc aagacggtgc tcatcgcgaa
     1561 gatgctccgc gacgagggcg ttcccgagga gcgcatccgg cgcgttcacg cgccgatcgg
     1621 cctcgatctc ggcggccgga cgccgggaga gatcgcgctc agcgtgctcg cggagatcag
     1681 cctcgagcgg ttcggcggga ccgggcgcgc gctgcgcctg ccggaagaga tccacgaccg
     1741 cacagtgagc ggacgccgct aacgcggccg gtcacaagcc aaaagcagac gcacaggcgc
     1801 cggcgggtgc ccgcgaccgc agcgggaccg tgttcgggct agtactgccc cgcgttcgcc
     1861 gacgcgacgg cgacgagcgc aagcgcggcg atcgtcgcga cgtagaggag gtacttgccg
     1921 agtttcacca ccccgctaac gtgacgcttc ctgcttcgct acgcgccgga gaacgcatcg
     1981 attactcgta cgccgtcctt agcagttaac gcgactcgcc gatctcgttg acgtcttgcg
     2041 ggtggggcgg ctcgttctcg gggcggtcgc tgggtgcggc gcgcgagccc cggcgggcgg
     2101 ccgtgatgcc ctcctcgcgc tccgacgcgc gctcgtcgtc catgcgccgg cccttgtcgg
     2161 gatcgccctc gtcgccctgc gggccggggc gttcctcgtt ctcttttcgc atgcgctcgt
     2221 ccggcgcacg gagcgtgcca aaccgcgctt cagaggttct gcaggatggt ccgggcgttg
     2281 ccgctggcga tgcggtcctt gtccgcctgc gggatgtcca gcgcctcgat gctcgacacg
     2341 ctgcgctcga tcgagccgag gaggtgtggg tagtcgctcc ccatcacgag atggtccgcg
     2401 ccgacctgcg cgatcgcgag ccggagggac ggcgcgctga acgacaccgt gtcgtagtag
     2461 agccgccgca ggtacacgct gggcagctcg tcgatgttcg cgcggcactc agcgaagtcg
     2521 cgccagccgt tgtcgagccg ctcaaagagg tagggcacgg cgccgcccag atgtcccagg
     2581 atccagcgga tccgcggcag ctgcttgaag acgccggcat agcagaggcg cgccacggcg
     2641 agcgtcgtgt cgaaggggaa tcccacgatc ggaccgagga cgtactccgc gaacgcctcc
     2701 gcgttcgccg ggatcatcgg atggatgaag acgcacgcgg accggcgatc gcactcctcg
     2761 aagaacggcc ggtagcgcgc gtcggtgagc gcccggccgt tgacgttcga cagcacgacg
     2821 acgccgcaga gacgcagctc gccgagcgcg cgatcgagct cgcgcagcgc cgcgtcgggg
     2881 tcgtccatcg ggatcgacgc gaagcccttg aagcgcgacg ggtgcttcgc gatgagctcc
     2941 gcatacgcgt cgttggcgag ccgcgcgacc tccgcctgcc gctcgggggg cgcgaagtac
     3001 acgttcggcg tcgagagcga cagcacctcg acatcgatgc ccacccggtc catgtcctcg
     3061 aggcgccgcg cggcatccgt catcggcggc gtgatgccga agaagcgcga accgcggaac
     3121 cggatgatcg tctggccggt cgggctctgt ccgaacgaga agtcgccgcc gacctcttcg
     3181 atcaggcgga agtaggactg cgggtagtag tgggtgtgca ggtcccagcg cggcatccgc
     3241 cgcgagggta gatcagggaa gggtggtcgg cttggccggg agcgtgaagt ccgagtcggg
     3301 caccgttgtc gagaggttcg tcgcctccat cgaccacgag gagccctgcg cggagaattg
     3361 cgagaggagc gcgatgccgt ctttgctata gcagaacgtg ccgctggtga agccggtcgc
     3421 ggcggaggcg accggcgtca cggcgtagca gaggccttgc tggccggcga tcgtccgcga
     3481 cggcgcggcg gtcgcgccgt aggactcggg gtgctcgatg agcgagcgct gcgtcaccgc
     3541 cgccatgttc tggtcgagtg gagagccgac gccgccgacg ctgaagcacg tcgcctgtcc
     3601 gcccgccgtc atgcagtaga aggtgccctc gggaagcgtg aagaacgaga tcgtcgactt
     3661 ctggccgccg gccgtcatgg caaagtcgaa gcgcgacctc ggcggcttga agtaccaggt
     3721 ctggtcgccc gagaatccct cggccccggc gcccgtcgcg gtgatcttgt aggtgacctt
     3781 gtactgcgcg agcttcgcgg cgcc
//
LOCUS       DASTZR010000499         2606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_336673, whole genome shotgun sequence.
ACCESSION   DASTZR010000499 DASTZR010000000
VERSION     DASTZR010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336673"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VFV20_11075"
     CDS             <1..353
                     /locus_tag="VFV20_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288936.1"
                     /translation="GNSGRAGFAIVRSRDGTPTVVVARDGVADEAWSPNGRLVGYTLI
                     TAQGSGTRRSAVVRDAETGDIVQETDGRFAGWSPDGSWTYVARAEGLYARRLAGGDPV
                     RFSPYGVVVAVTKP"
     gene            complement(340..1221)
                     /locus_tag="VFV20_11080"
     CDS             complement(340..1221)
                     /locus_tag="VFV20_11080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEU5288937.1"
                     /translation="MRVGVVFPQLESGTDPGAIREYAQGVEQLGYAHLVAYDHVLGAD
                     RSARPDYRGAYDSTSLFHEVFVLLSFIAAWTTRLELAPSVVILPQRQTALVAKQAASL
                     DVLAGGRTRLGVGLGWNEVEFVGLNERFDTRAKRYSEQIKVLRMLFANEVVDFTGTYH
                     RIDRAGIRPLPVRRAIPIWMGGNAEDAVKRIARIGDGWFTFAQPDGDGPERIARFREY
                     VRAAGRDPETFPIEGRVVFAKRTPDEWASRARGFADLRTTHLEVNTMGAGYMSLADHL
                     EALARFRRDATELFTAS"
     gene            1257..1559
                     /locus_tag="VFV20_11085"
     CDS             1257..1559
                     /locus_tag="VFV20_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5288938.1"
                     /translation="MDVEHVAHASEAADPASIEARVRAEARDVYEWSNGPGDRYAEHA
                     HPYVKLLYCMRGAIDFILADGRTVSLRAGDRLVLPAGTRHAAVVGPDGCACVEGKL"
     gene            1581..>2606
                     /locus_tag="VFV20_11090"
     CDS             1581..>2606
                     /locus_tag="VFV20_11090"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU5288939.1"
                     /translation="MRPLARRMGRLGTETAFEVLARARALEAQGRHIVHLEIGEPDFD
                     TPRSITAAGVAAIERGETHYTPSAGVPELRQAIADYLARTRGLAYDASQVIVAPGAKP
                     IMFYAILALVESGDEAIFPDPGFPIYASMISFAGGTPVSLPLREQNDFVPDLDELRRL
                     VTPKTKLIILNSPHNPTGSALSHEAVREIARIARDHDLWVLSDEIYADLLYDGTHHPI
                     GREDGMLDRTIVLDGFSKTFAMTGWRLGYGAFPKPLVEPVTKLVTNSVSCTATFTQRA
                     GIVALTQRPPEVAEMVAAFRQRRDTIVAGLNSIDGISCRTPAGAFYVFPSITGLGLGN
                     AATVAGRM"
BASE COUNT          380 a          930 c          914 g          382 t
ORIGIN      
        1 cggggaactc gggccgggcc gggttcgcga tcgtgcgatc gcgcgacggt accccgaccg
       61 tcgtcgtcgc gcgcgacggc gtggccgacg aggcatggtc gccgaacggt cgcctcgtcg
      121 ggtacaccct gatcacggct caggggtccg gcacccgcag aagcgcggtc gtgcgggacg
      181 cggagaccgg cgacatcgtg caggagaccg acggacggtt cgccggctgg tccccggatg
      241 gctcgtggac ctacgtcgcg cgcgccgagg gcctgtacgc gcgacgactc gccggtggcg
      301 accccgtgcg cttctcgccc tacggcgtcg tggtcgcggt cacgaagccg tgaacagctc
      361 cgtcgcgtcg cggcggaatc gggcgagcgc ctcgaggtgg tcggccagac tcatgtagcc
      421 cgcgcccatc gtgttcacct cgaggtgcgt cgtgcgcagg tccgcgaagc cgcgcgctct
      481 gctcgcccac tcgtccggcg tgcgcttcgc gaacacgacg cggccctcga tcgggaacgt
      541 ctcgggatcg cgacccgccg cacggacgta ttcgcggaag cgtgcgatgc gctccggtcc
      601 gtcgccgtcg ggctgcgcga aggtgaacca gccgtccccg atgcgcgcga tgcgcttcac
      661 cgcgtcctcg gcgttcccgc ccatccagat cgggatcgcc cgccggaccg gcagcggcct
      721 gatgcccgcg cgatcgatgc ggtggtacgt cccggtgaag tcgaccacct cgttcgcgaa
      781 gagcatccga agcaccttga tctgctcgct gtatcgcttc gcgcgggtgt cgaagcgctc
      841 gttcagtccg acgaactcca cctcgttcca gccgagcccg acccccagcc gcgtgcgccc
      901 gcccgcgagc acgtcgagcg acgcggcctg cttcgcgacg agcgcggtct gccgctgcgg
      961 gaggatcacg accgagggcg cgagctcgag ccgcgtggtc catgcggcga tgaacgagag
     1021 caggacgaag acctcgtgga aaagggaggt ggagtcgtag gcgccgcggt aatcgggacg
     1081 cgcggagcgg tcggcgccga ggacgtggtc gtacgcgacc aggtgcgcgt agccgagctg
     1141 ctccaccccc tgcgcgtatt cgcggatggc acccggatcg gttccgctct cgagctgcgg
     1201 gaagaccacg cccacgcgca cggtccgcag tatgtcgcgg ccgctatcgt cgcgccgtgg
     1261 acgtagagca tgtggcgcat gcgagcgagg cggcggatcc ggcctcgatc gaggcgcgcg
     1321 tccgcgcgga ggcgcgcgac gtctacgagt ggtcgaacgg accgggtgac cgctacgccg
     1381 agcacgcgca tccctacgtg aagctcctgt actgcatgcg cggcgcgatc gacttcatcc
     1441 tcgccgacgg caggacggtg agcctgcgag cgggcgaccg tctcgtgctt ccggcgggca
     1501 cgcgccacgc cgcggtggtc ggaccggacg gctgcgcgtg cgtggagggc aagctctagc
     1561 gccgggctag actccgagcg atgcgtccgc tcgcgcgccg catggggcgg ctcggcacgg
     1621 agacggcgtt cgaggtgctc gcgcgcgcac gcgcgctcga ggcccagggc cgtcacatcg
     1681 tgcatctcga gatcggtgag cccgacttcg acacgccacg gtccatcacc gcggccggcg
     1741 tcgccgcgat cgagcgcggc gagacgcact acaccccgtc cgccggcgtg cccgagctgc
     1801 gccaggcgat cgcggactat ctggctcgca cccgcgggct cgcgtacgac gcgtcgcagg
     1861 tcatcgtcgc ccccggcgcg aagccgatca tgttctacgc gatcctcgcc ctcgtcgaga
     1921 gcggcgacga ggcgatcttc ccggatcccg gcttcccgat ctacgcgtcg atgatcagct
     1981 tcgccggcgg aacgccggtg tcgctgccgc tgcgtgagca gaacgacttc gtgcccgatc
     2041 tcgacgagct gcgccgtctc gtgacgccga agacgaagct catcatcctc aacagcccgc
     2101 acaaccccac gggcagcgcg ctctcgcacg aggccgtgcg cgagatcgcg cgcatcgcgc
     2161 gggatcacga cctgtgggtc ctctccgacg agatctacgc cgaccttctc tatgacggca
     2221 cgcatcatcc gatcggccgc gaggacggca tgctggatcg cacgatcgtg ctggacggct
     2281 tcagcaagac cttcgcgatg accgggtggc ggctgggcta cggcgccttc ccaaagccgc
     2341 tcgtggagcc ggtgaccaag ctcgtgacga actcggtgtc gtgcaccgcc acgttcacac
     2401 agcgcgcggg catcgtcgcg ctcacgcagc gtccgcccga ggtcgcggag atggtcgcgg
     2461 cattccggca gcgccgcgac acgatcgtcg caggactgaa ctcgatcgac gggatcagct
     2521 gccgtacgcc ggccggcgcg ttctacgtct tcccgagcat cacgggcctc ggtctgggca
     2581 acgccgcgac cgtcgccggc cgcatg
//
LOCUS       DASTZR010000500         2454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_860577, whole genome shotgun sequence.
ACCESSION   DASTZR010000500 DASTZR010000000
VERSION     DASTZR010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860577"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="VFV20_11095"
     CDS             <1..716
                     /locus_tag="VFV20_11095"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEU5288940.1"
                     /translation="TIASFISYAVERATSRRKSEFGKGAIEGVAGPEAANNASAGGAL
                     VPLLGLGIPGSGTTAVMVAAFQIYGLQPGPLLFDKRPDLVWGLIASLYLANVMLLVLN
                     LPLVGMWVRLLDLPKPLLFSGIIVISAIGVYSLDRRPSDLIVVFALGIIGYLMRRYDY
                     PLAPVILGIVLGPLIDNNFRRAMIMTDGDIVAMLSRPAAASILAVAVVFFALPYAIRI
                     YGARRGNRAVAALAETDVG"
     gene            complement(713..1378)
                     /locus_tag="VFV20_11100"
     CDS             complement(713..1378)
                     /locus_tag="VFV20_11100"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEU5288941.1"
                     /translation="MRASDIVPIHVADFTYPSGHPLAGQAGLVLCFVVRHPGGLVLVD
                     TGFVEGHPSIDEHYRPRGRAIREAIGAAGIDATGIRAVVNTHLHFDHAGQNAAFPDVP
                     IIVQDLEWDAAWERDDHTIREWIDFPNASYVRVSGDTELAPGLRALATPGHTPGHQSV
                     TVDTDDGLVLIVGQAAQDARGFATGEADASLARLRALNAERIHFSHDRAVLKRTAPHA
                     AQN"
     gene            1477..1908
                     /locus_tag="VFV20_11105"
     CDS             1477..1908
                     /locus_tag="VFV20_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288942.1"
                     /translation="MPILSDDIKAQLKERLATSLRGPVELRLRTRPSAGPLIIPGAET
                     CETCDPCREIAEGLAEASRLVRLVVTEDRGVTAPVIEIAPPGEPARITFEGLPSGYEF
                     AGLLDAIERVSSADSGLSEKTRTALAALEQDVELMVFVTPT"
     gene            1921..2145
                     /locus_tag="VFV20_11110"
                     /pseudo
     CDS             1921..2145
                     /locus_tag="VFV20_11110"
                     /inference="COORDINATES: protein motif:HMM:NF024590.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
     gene            complement(2142..>2454)
                     /locus_tag="VFV20_11115"
     CDS             complement(2142..>2454)
                     /locus_tag="VFV20_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020478229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEU5288943.1"
                     /translation="NYPALGADAFRTGTGVHAAAVIKALRRGDRELADLVYSGVPASV
                     VGREQRIEIGPMSGESNVVFWLESHGFAAEPERVARLMEAAKRSDRLLSEDEILALAR
                     P"
BASE COUNT          337 a          875 c          852 g          390 t
ORIGIN      
        1 cgacgatcgc gtcgttcatc tcgtacgcgg tggagcgcgc gacgagccgc cgcaagtccg
       61 agttcggcaa gggcgcgatc gagggcgtcg ccggtcccga ggccgcgaac aacgcgtcgg
      121 cgggtggcgc gctcgtgccg ctcctcggcc tcggcatccc gggctcgggc acgaccgcgg
      181 tcatggtggc cgcgttccag atctacggcc tgcagcctgg accgctgctg ttcgacaagc
      241 ggccggacct ggtgtgggga ctcatcgcga gcctctacct cgcgaacgtc atgctcctcg
      301 tgctcaacct gccgctcgtc ggcatgtggg tccgactcct cgacctgccg aagccgctgc
      361 tcttctcggg gatcatcgtg atctcggcga tcggcgtgta ctcgctcgat cgccgtccgt
      421 cggatctcat cgtcgtcttc gcgctcggca tcatcgggta cctcatgcgc cgctacgact
      481 atccgctcgc gcccgtgatc ctgggcatcg tgctcggacc gctcatcgac aacaacttcc
      541 gtcgcgcgat gatcatgacc gacggcgaca tcgtcgcaat gctgtcgcgg cccgcggcgg
      601 cgtcgatcct cgccgtcgcc gtcgtcttct tcgcgctgcc gtacgccatc cgcatctacg
      661 gcgcgcgccg cggcaaccgc gcggtggccg cgctggccga gaccgacgtc ggctagttct
      721 gcgcggcgtg cggcgcggtg cgcttcagca ccgcgcggtc gtgcgagaaa tggatgcgct
      781 cggcgttcag cgctcggagc cgagcgagcg aggcgtccgc ctcgccggtg gcgaagcccc
      841 gcgcatcctg cgcggcctga ccgacgatga gcacgaggcc gtcgtcggtg tcgacggtga
      901 ccgactggtg gccgggcgtg tgccccggcg tcgcgagcgc gcgcagcccc ggcgcgagct
      961 cggtgtctcc gctcacgcgg acgtagctcg cgttcgggaa gtcgatccac tcacggatcg
     1021 tgtgatcgtc gcgctcccat gcggcatccc actcgaggtc ctgcacgatg atcggaacgt
     1081 ccgggaacgc ggcgttctgt cccgcgtgat cgaaatgcag gtgtgtgttc acgaccgcgc
     1141 ggatgccggt cgcatcgatg cccgcggcgc cgatggcctc gcggatggcg cggccgcgcg
     1201 gacggtagtg ctcgtcgatc gatggatgtc cttcgacgaa cccggtgtcg acgagcacca
     1261 gtccgcccgg gtgtcgcacc acgaagcaga ggaccaggcc ggcctggccg gcgagcggat
     1321 gcccggacgg atacgtgaag tcggcgacgt ggatcggaac gatgtcgctc gcgcgcacgg
     1381 tcccagtctc ccatgtgggg cggcgcagga cagcgccgcg ccgctcgcgg aatacacggc
     1441 accggccgcg tgtctttagc gatggaggtg cgatccgtgc ccatcctcag tgacgacatc
     1501 aaagcgcagc tcaaagagcg cctcgccacg agcctgcgcg ggccggtcga gctgcgtctg
     1561 cgcacgcggc catccgccgg gccgctcatc atccccggag cggaaacctg cgagacctgc
     1621 gacccgtgcc gcgagatcgc ggaaggcctc gccgaggcct ccaggctcgt gcggctggtc
     1681 gtgaccgagg atcgtggcgt gaccgcaccc gtcatcgaga tcgcgccccc tggcgagccg
     1741 gcgcgcatca cgttcgaagg cctgccgtcc ggctatgagt tcgccggtct gctcgacgcg
     1801 atcgagcgcg tctcgagcgc ggactccggg ctgagtgaga agacgcgcac ggcgctcgcc
     1861 gccctcgaac aggacgtcga gctcatggtg ttcgtcacac ccacctgacc gtactgcccg
     1921 agcgcggtcg cgctcgcgaa tcgcatgacg gtcgcctcac cacgcgtccg ggcacgcgcc
     1981 atcgaggcga atgagttccc cgagctgtcg cagcgctatg gcgtgcgcgg cgttccgcag
     2041 acggtcgtga acggcagcgg catcttcgtc ggcgcgctgc ctgagccggc attcctcgag
     2101 caggtgctcg cctacgccgg cgcgccggcc acggacgagc cctaggggcg cgcgagcgcg
     2161 aggatctcgt cctcgctgag cagacggtcg cttcgcttcg cggcctccat gaggcgcgcg
     2221 acgcgctcgg gctccgcggc gaagccgtgc gactccagcc agaagacgac gttcgattcg
     2281 cccgacatgg gtccgatctc gatccgctgc tcgcgtccga ccacgctcgc cggaacgccg
     2341 ctgtacacga ggtcggcgag ctcgcggtcg ccgcggcgca gcgccttgat gacggcggcg
     2401 gcgtgcacgc ccgtgccggt gcggaacgcg tcggccccga gcgccgggta gttc
//
LOCUS       DASTZR010000501         2308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_990092, whole genome shotgun sequence.
ACCESSION   DASTZR010000501 DASTZR010000000
VERSION     DASTZR010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990092"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1749
                     /locus_tag="VFV20_11120"
     CDS             <1..1749
                     /locus_tag="VFV20_11120"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEU5288944.1"
                     /translation="VAIELAEANASWRAQRQGQELEGRLRAARELQRSLRQHDPNAVL
                     DRATNQIAELFGADGVSVMLVDAEGRLAVRSARGLSDEAKRDRKVIGEGISGTVAKSG
                     QPTILAGPVAGGSDATVSEAMIVPLRIGDRTLGVVNVKHRSPQQKYTQTQLDSLVLVA
                     QDVASAYTTAEELRRAEDDRQQAIVLYELSRFATLGTDVQGDLGAAVAMLAGTLHHDA
                     AAVWQHENDRLRLRAATGYDAPAESEIAVDEADTTLATVLREKHTHRVQIGVADERPE
                     WAAPGCTHFVLAPIAGIGALVVGRATLPYTDAERDFATTVADYLVGMLQKSSAQDVVD
                     RGIATERRRIAQEIHDGLAQELTGVVLALEGCQRAFEKDPALLSPQLAKASRDARATL
                     ADVRQYMAALRQSETGALNLPVTLARLVDDLRRQTGLQVEMDESGAQRELEPIVERAV
                     IRIVGEALRNVAQHAGAQKAKVALLYGAEGVVVTVEDDGKGFDVEATFAKAEGSGHFG
                     VVGMRERAEAAGGQLVVRSEAGRGTIVRASIPYANFAAVPGMTNGPIEDVERQPGIED
                     QDTAERSGFLGRLFGR"
     gene            1746..>2308
                     /locus_tag="VFV20_11125"
     CDS             1746..>2308
                     /locus_tag="VFV20_11125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5288945.1"
                     /translation="MTGPVRNVRVLIADDHAVVRSGLRSILDHEADIEVVGEAADGHQ
                     ALEQAQELLPDVILMDINMGDWDGVTATRRVRNSVPSSRVIVLTNYDEDNLVFSSIRA
                     GASGYLLKEVSGHQLTNAIRTVADGFSLIYPSVARRVLDEFGRLRGGGPAAADDDQGY
                     SDLTPREREVLKLVASGRANKEIGSELG"
BASE COUNT          373 a          791 c          846 g          298 t
ORIGIN      
        1 gtcgcgatcg agctcgcgga ggcgaacgcg tcgtggcgcg cgcagcgaca gggacaggag
       61 ctcgagggaa ggctccgcgc ggcgcgcgag ctgcagcggt cgctccggca gcacgatccg
      121 aacgcggtcc tggatcgcgc cacgaaccag atcgcggagc tctttggcgc ggacggcgtg
      181 tcggtcatgc tcgtcgacgc cgagggccgg ctggcggtgc gcagcgcgcg cggcctctcc
      241 gacgaagcga agcgcgaccg caaggtcatc ggcgagggca tcagtggaac ggtggcgaag
      301 agcgggcagc ccacgatcct cgcgggccct gtggcgggcg gcagcgacgc gaccgtcagc
      361 gaggcgatga tcgtcccgct gcgcatcggt gaccggacgc tgggcgtcgt caacgtgaag
      421 caccgctcgc cgcagcagaa gtacacgcag acgcagctcg actcgctcgt gctggtggcg
      481 caggacgtgg cgagcgcgta cacgaccgcc gaggagctgc ggcgcgccga ggacgatcgc
      541 cagcaggcca tcgtgctgta tgagctgtct cgtttcgcga cgctcgggac ggacgtacag
      601 ggcgatctcg gggccgcggt cgcgatgctc gcgggtacgc tccaccacga cgccgcggcg
      661 gtatggcagc acgagaacga ccgactgcgg cttcgcgcgg cgaccggata cgacgcgccc
      721 gcggagtcgg agatcgcggt cgatgaggcg gacacgacgc tcgcgacggt gctgcgcgag
      781 aagcacacgc accgcgtgca gatcggggtc gccgacgagc ggccggaatg ggccgcgccc
      841 ggctgcacgc acttcgtcct cgcgcccatc gcgggcatcg gcgccctcgt cgtcggtcgc
      901 gcgacgcttc cctacaccga cgcggagcgc gacttcgcga cgacagtggc cgactacctc
      961 gtcggcatgc tgcagaagtc ctcggcccag gatgtcgtgg accgcggcat cgccacggag
     1021 cgccgacgca tcgcgcagga gatccacgac ggcctggcgc aggagctcac cggtgtcgtc
     1081 ctcgcgctcg aggggtgcca gcgggcgttc gagaaggatc ccgcgctgct cagcccccag
     1141 ctcgcgaagg cctcacgcga cgcgcgcgcc acgctggcgg acgtccgcca atacatggcc
     1201 gcgctccgcc agagcgagac cggggcgctc aaccttcccg tcaccctggc ccgtctggtc
     1261 gacgatctgc gccgccagac cggcctccag gtcgagatgg acgagtcggg cgcgcagcgt
     1321 gagctcgagc cgatcgtcga gcgtgcggtc atccgcatcg tgggtgaggc gctacgcaat
     1381 gtcgcgcagc acgccggtgc gcagaaggca aaggtcgcac tgctctacgg ggcggagggc
     1441 gtcgtcgtga ccgtcgagga cgacggcaag ggattcgacg tcgaggccac cttcgcgaag
     1501 gcggagggga gcggtcactt cggcgtcgtc ggcatgcgcg agcgcgccga ggcggcgggc
     1561 gggcagctcg tcgtgcgcag cgaggcgggc cgcggcacga tcgtgcgcgc gagcatcccc
     1621 tacgcgaact tcgccgcggt accaggaatg acgaacggtc cgatcgagga cgtcgagcgc
     1681 cagcccggca tcgaagacca ggacacagcg gagcgcagcg gcttcctcgg aaggctgttc
     1741 ggccgatgac ggggcccgtg cggaacgtgc gcgtgctcat cgccgacgac cacgccgtgg
     1801 tccgcagcgg actgcgctcg atcctcgatc acgaggcgga catcgaggtc gtcggtgagg
     1861 ccgccgacgg tcatcaggct ctcgagcagg cccaggagct gctgcccgac gtcatcctca
     1921 tggacatcaa catgggcgac tgggacggag tgaccgcgac gcggcgcgtg cggaactcgg
     1981 tcccgtcatc tcgcgtcatc gtgctcacga actacgacga ggacaacctc gtgttctcct
     2041 cgatccgcgc cggcgcatcc ggctacctcc tcaaggaggt gagcgggcat cagctcacga
     2101 acgcgatccg caccgtcgcc gacggcttct cgctcatcta cccgtcggtc gcgcgccgcg
     2161 tgctcgacga attcggccgg cttcggggcg gcggtccggc cgcggctgac gacgaccagg
     2221 gctacagcga cctcaccccg cgcgagcgcg aggtcctgaa gctcgtcgcg agcggccgcg
     2281 cgaacaagga gatcggcagc gagctcgg
//
LOCUS       DASTZR010000502         2068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_76599, whole genome shotgun sequence.
ACCESSION   DASTZR010000502 DASTZR010000000
VERSION     DASTZR010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76599"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VFV20_11130"
     CDS             <1..272
                     /locus_tag="VFV20_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288946.1"
                     /translation="DAASHKPSPTPTPKPTAKATPVPAPTVWTAYVELLVKDCLVKNA
                     AATATPGSTQAIAAAGEACQKAITATGLAPRDFWARFGVSQPAKN"
     gene            362..>2068
                     /locus_tag="VFV20_11135"
     CDS             362..>2068
                     /locus_tag="VFV20_11135"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5288947.1"
                     /translation="MAAITETAEAYPKHREAHVVLRDGSLVLLRPVTPMDQDAIAQLL
                     ASLSPAARHNRFFSSSTDLAAEARRAVDVDQRSRYALVAVGVDGRVIAHAAYFAEDSG
                     PAEVAWIVADDHKGRGLGTLMLAHLAERAEEVGVGPFVAYVLPDNREMIEVLRESGVP
                     MRTTRSHDQLAFEFPTSLSSAARDRFDRREQVATAASIGALLAPRSIAVVGASRSRGT
                     IGGEIFHNLLATGFAGPVYPVNPKANVVQSVPAYASIADVPGPVDLAVIVVPAADVLA
                     AARGCAEKGVRGLVVISAGFGETGPAGLARQQELVELCRASGMRLIGPNCMGVLNTAA
                     SVRMNATFAPTFPPPGRVGFLSQSGALGLAIIDQCAERGIGLSTFVSVGNKADISGND
                     LLQYWETDPDTDLVLLYLESFGNPRKFDRIARRIGRTKPVLVVKSGRSVAGVRATSSH
                     TGALVASSDVTVDALFAQTGVVRTDTLAELLDAAALFATQPLPAGSRVGIVTNAGGPA
                     ILCADALESRGLDVPALPDDLKATLRTMLPAEASVANPVDMIASASGEQYAQVVRALA
                     ASG"
BASE COUNT          285 a          792 c          699 g          292 t
ORIGIN      
        1 gcgacgccgc atcgcacaag ccgtcgccga ctccgacgcc gaagcccacc gcgaaggcga
       61 cgccggtgcc ggcaccgacc gtctggacgg cgtacgtcga gctcctggtg aaggactgcc
      121 tggtgaagaa cgcggccgcg accgcgacgc ccggatccac ccaggcgatc gccgccgcgg
      181 gcgaggcctg ccagaaggcg atcacggcga ccggactcgc accgcgcgac ttctgggctc
      241 ggttcggcgt ctctcagcca gccaagaact agtctccgtc cggcgcttta cgtcccaact
      301 cccgctcgct ccgggacctt cggcactgcc tgggcggtgg cgccaagcgg acgctacgcg
      361 gatggcggcc atcaccgaga ccgcggaggc ctacccaaag catcgcgagg cgcacgtcgt
      421 gctgcgcgac ggctcgctcg tgctgctccg cccggtcacg cccatggacc aggacgcgat
      481 cgcgcagctc ctcgcctcgc tctcgcccgc cgcccgccac aaccggttct tctccagcag
      541 caccgacctc gcggccgagg cacggcgcgc ggtggacgtc gatcagcgct cgcgctacgc
      601 gctcgtcgcg gtcggcgtcg acgggcgggt catcgcccac gcggcgtact tcgcggagga
      661 cagcggaccc gcggaggtcg catggatcgt cgcagacgac cacaagggac gcgggctcgg
      721 aacgctcatg ctcgcccacc tcgccgagcg cgccgaggag gtcggcgtgg gcccgttcgt
      781 cgcctacgtc ctccccgaca accgcgagat gatcgaggtc ctccgcgaga gcggcgtccc
      841 gatgcgcacc acgcggtcgc acgatcagct cgccttcgag ttccccacgt ccctctcgag
      901 cgccgcgcgc gaccgcttcg accgccgcga gcaggtcgcg accgcggcca gcatcggcgc
      961 gctgctcgcg ccacgctcga tcgcggtcgt cggcgcctcg cggtcgcgcg gcacgatcgg
     1021 cggcgagatc ttccacaacc tgctcgccac cggattcgct gggccggtct atccggtgaa
     1081 tccgaaggcg aacgtggtgc agtcggtccc ggcgtatgcc tcgatcgctg acgtgcccgg
     1141 cccggtggac ctcgccgtca tcgtcgttcc cgccgccgac gtccttgcgg cggcgcgcgg
     1201 ctgcgccgaa aagggcgtcc gcggcctggt ggtcatctcg gccggattcg gcgagaccgg
     1261 cccggcggga ctcgcgcggc agcaggagct cgtcgagctc tgccgcgcgt cgggcatgcg
     1321 cctcatcggg ccgaactgca tgggcgtgct caacaccgcg gcgagcgtgc ggatgaacgc
     1381 gacgttcgcg ccgacattcc cgcctcccgg ccgcgtgggc ttcctctcgc agagcggcgc
     1441 gctcggcctc gcgatcatcg accagtgcgc cgagcgcggc atcggacttt ccacgttcgt
     1501 ctccgtcggc aacaaggcgg acatctcggg caacgacctg ctccagtact gggagaccga
     1561 tccggacacc gacctcgtcc ttctgtacct cgagtctttc ggcaacccgc ggaagttcga
     1621 ccgcatcgcg cggcgcatcg gccgcaccaa gccggtcctc gtcgtgaaga gcggccggtc
     1681 ggtcgcgggc gtgcgcgcca cgtcgtcgca caccggcgcc ctcgtggcgt cgtcggacgt
     1741 cacggtggat gcgctgttcg cgcagaccgg cgtggtgcgc acggacacgc tcgccgagct
     1801 gctcgacgcc gccgcgctct tcgcgacgca gccgctgccc gcggggagcc gcgtcggcat
     1861 cgtcaccaac gccggcggcc ccgcgatcct ctgcgccgac gcgctcgagt ctcgaggcct
     1921 cgacgtgccc gcattgccgg acgacctgaa ggccacgctt cgcacgatgc tgcccgcgga
     1981 ggcatcggtc gcgaacccgg tggacatgat cgcgtccgca tccggcgagc agtacgcaca
     2041 ggtggtccgc gcgctcgcgg catccgga
//
LOCUS       DASTZR010000503         2633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_337082, whole genome shotgun sequence.
ACCESSION   DASTZR010000503 DASTZR010000000
VERSION     DASTZR010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337082"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..328
                     /locus_tag="VFV20_11140"
                     /pseudo
     CDS             1..328
                     /locus_tag="VFV20_11140"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine dehydrogenase"
     gene            337..1731
                     /locus_tag="VFV20_11145"
     CDS             337..1731
                     /locus_tag="VFV20_11145"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU5288948.1"
                     /translation="MTRTVLKFGGTSVASPTALESLARIVGSLTGDRIVVVSATAGTT
                     DALHTAAGEATSGHAANAERVMQDLAKAHGNLVADLLGVGGAETLREITDLTERTVGL
                     LRSVAVLRECTPRSLDAIVTYGERVSAPIVAALLTARGTRAEGLSAEGLIVTDDQFGH
                     ANPLMDETRARVQKELLPRLKGGIVPVVTGYVASTADGVTTTLGRGGSDYSAAVLAAA
                     VNATELRIYTDVSGVMSADPRIVKDARPLAHMSYAEAAELSYFGAKVIHPRTVLPAIE
                     ARIPVRILNTFSPADQGTTITADPAPDGSVVKATTSLGDLALVTVQGAGMSGVPGFAA
                     RVFDTTAAEHVSVLMISQSSSENSICLLVPAEFAGRLRPALERIFSAEMRRHDVERVT
                     VDAPVAIVAAVGEGMRGTPGVAARVFGALGRAKVNVMAIAQGSSEENISLVVAQADRD
                     AAVRAIHDEFHAAH"
     gene            1736..>2633
                     /gene="asd"
                     /locus_tag="VFV20_11150"
     CDS             1736..>2633
                     /gene="asd"
                     /locus_tag="VFV20_11150"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF006416.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEU5288949.1"
                     /translation="MKKRKVAVLGATGAVGQRFIALLAEHPWFEIAALTTSDAKAGKR
                     YGDVTAWHFGADMPAAVADMALVATTDDLDAEICFSALPSEAAERWEAALAKTGHHVF
                     SNVKTHREDPDVPLIIAEVNPDHAAALDVQRKNRGWSGSIVTNGNCSAITFALAAAPV
                     HRAFGIERAVVTTMQALSGAGYPGVASLDALDNVVPFIGEEEEKMERETKKFLGAWDG
                     ARFADAPIAFSAHCNRVSVRDGHTEAVSVTLRDKASVGEIVDAMRTFRGRPQELRLPT
                     APACPIVVRTERDRPQPVKDRDT"
BASE COUNT          392 a          958 c          944 g          339 t
ORIGIN      
        1 gtcgctgctg gagggccggc acttccatcc cgacaacgtc cggcgcgcga cgctccgcgg
       61 gctctcggtc gcggacgtcg ctgcggcgcg ggcaaagggc gccacgatcc gctacctcgc
      121 gacggcggac ttcggcgaga aggcgacgaa ggcgcgggtg ggacccgagg aggtcacgct
      181 gggatcgctg gagggacagg ccgccgggcc gacgaacgtg gtgcgcatcg agactgacat
      241 cgcggggacg ctcgtgttca gcggtccggg cgcgggcggc gaggccaccg cgtcggccat
      301 actcggcgac gtgatcgcga tcgccagggt cgcacgatga cccgcaccgt cctcaagttc
      361 ggcggcacgt ccgtcgcatc gccgaccgcg ctcgagagcc tggcgcgcat cgtcggatcg
      421 ctcaccggcg accggatcgt cgtcgtgtcc gcgaccgcgg ggaccaccga cgcgctccac
      481 accgcggcgg gcgaggcgac gagcgggcac gcggcgaacg ccgagcgcgt catgcaggac
      541 ctggcgaagg cgcacggcaa cctcgtcgcc gatctcctcg gcgtcggcgg cgccgagacc
      601 ctgcgcgaga tcaccgatct caccgagcgc accgtcggac tgctgcgcag cgtcgcggtg
      661 ctgcgcgagt gcacgccgcg atcgctcgac gcgatcgtca cgtacggcga gcgcgtgtcg
      721 gcacccatcg tcgcggcgct gctcaccgca cgcggcacgc gcgcggaagg gctctcggcc
      781 gaggggctca tcgtcacgga cgaccagttc ggtcacgcca acccgctcat ggacgagacc
      841 cgggcgcgcg tccaaaagga gctcctcccg cgcctcaagg gcggcatcgt cccggtcgtg
      901 accggctacg tcgcttcgac cgccgacggc gtcaccacga cgctcgggcg cgggggctcc
      961 gattactccg ccgcggtgct cgccgcggcc gtgaacgcga ccgagctgcg catctacacc
     1021 gacgtgagcg gcgtcatgag cgccgacccg cgcatcgtga aggacgcgcg gccgctcgcg
     1081 cacatgtcgt acgcggaggc cgccgagctg tcgtacttcg gcgcgaaggt catccatccg
     1141 cggacggtgc tcccggcgat cgaggcgcgc atcccggtcc gcatcctcaa cacgttctcg
     1201 cccgcggacc aggggaccac catcaccgcc gaccccgcgc cggacggcag cgtggtgaag
     1261 gcgacgacgt cgctcggcga cctcgcgctc gtcacggtgc agggcgccgg gatgagcggt
     1321 gtcccggggt tcgcggcgcg cgtgttcgac acgacggccg cggagcacgt ctcggtcctc
     1381 atgatcagcc agtcatcgtc cgagaactcg atctgcctgc tcgtgccggc cgagttcgcg
     1441 ggccggctgc gccctgcgct cgagcggatc ttcagcgccg agatgcggcg gcacgacgtc
     1501 gagcgggtca cggtggacgc accggtcgcg atcgtcgcgg ccgtggggga gggcatgcgc
     1561 gggacgcccg gcgtcgcggc gcgcgtcttc ggcgcgctcg gccgggccaa ggtgaacgtg
     1621 atggcgatcg cgcagggatc gagcgaagag aacatctcgc tcgtcgtggc gcaggccgac
     1681 cgcgacgcgg ccgtccgggc gatccacgac gagttccatg ccgcacacta gtcgcgtgaa
     1741 gaagcggaag gtcgcggtcc tcggagccac gggcgcggtc gggcagcgct tcatcgcgct
     1801 gctcgccgag catccgtggt tcgagatcgc cgccctcacc acctcggacg ccaaggcggg
     1861 caagcgctac ggcgacgtga ccgcatggca cttcggcgcc gacatgcccg cggccgtcgc
     1921 cgacatggcg ctcgtcgcca ccacggacga cctcgacgcc gagatctgct tctccgcgct
     1981 gccgtccgag gccgcggagc gatgggaggc cgccctcgcg aagaccggcc accacgtctt
     2041 ctcgaacgtg aagacgcacc gcgaggaccc ggacgtcccg ctcatcatcg cggaggtcaa
     2101 ccccgaccac gccgccgcac tcgacgtgca gcggaagaac cgcggctggt ccggttccat
     2161 cgtgaccaac ggcaactgct cggcgatcac attcgcgctc gcggccgcgc cggtccaccg
     2221 cgcgttcggc atcgagcgcg cggtcgtgac cacgatgcag gcgctctcgg gcgcgggcta
     2281 tccgggagtg gcgtcgctcg acgcgctcga caacgtcgtc cccttcatcg gcgaagagga
     2341 agagaagatg gagcgcgaga cgaagaagtt cctcggcgcc tgggacggcg cgcgcttcgc
     2401 cgacgcgccg atcgcgttct ccgcccactg caaccgcgtc tcggtgcgcg acggccacac
     2461 cgaggccgtg tccgtgacgc tccgcgacaa ggcgagcgtg ggggagatcg tcgacgcgat
     2521 gcgcaccttc cgcggccggc cgcaggagct gcggctaccg acggcccctg cgtgtccgat
     2581 cgtcgtgcgc accgagcgtg accggccgca gccggtgaag gaccgcgaca ccg
//
LOCUS       DASTZR010000504         2871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_1303758, whole genome shotgun sequence.
ACCESSION   DASTZR010000504 DASTZR010000000
VERSION     DASTZR010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303758"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VFV20_11155"
     CDS             <1..484
                     /locus_tag="VFV20_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEU5288950.1"
                     /translation="ALVAAFRATLEELDDADLLLHVVDAGHPNLHERLIVVKETLGTL
                     GLADRPSLLVFNKADTLAGAEGGALREALGGEFPGAVFTAARTGAGLDALRARLAELA
                     AAGWQHVKVVVPYRDGALVQRVRERGALRGTQYGESGIEIEADVPPDLASELTHRHSA
                     "
     gene            509..>2871
                     /locus_tag="VFV20_11160"
     CDS             509..>2871
                     /locus_tag="VFV20_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288951.1"
                     /translation="MGKTLVRVALAAFLAISSVPFVSRPSVAASCAATGVPTRTIYLP
                     NITKTLGGPAGWVTPFIVQNVGVAPTDLEISFYRFSDGGLVTCRRISGLAPYRSFADV
                     PNNDVDLPADSQFAVVVRSYGAEVISVVNEHQGAGATAEALSYVGLARGAQTQALPYV
                     AKFASGWLVTFVMQNLGVANANVTARFVSFDGSKTATLTRVIAPGRSQFVNPVAEPSL
                     ATGTEYSVMLTSDQPIAAIANAHNDLPGSAAPMGFSYNAVASLSTEVMYVPSVARNAD
                     GVGRTTRVVVQNTGAFDAAPTLVFRRPGAPSVTVNVPTAIRAGRSWAFDPRLFADGIT
                     PCPATGAANCVGEGDWSLTVSGGQFAVLAIATTSATALGSVGIAAPGNRAYLPNVTRT
                     LGGGLGWTTPIVLQSAGATSATLRWYRFVDGVLVLRQTVALSNGLAIRIDPRAVGGLA
                     DDTQYAVVVDAQGGDVAASVVELSFLGGDGAMAYEGFGATVATTPTATMISLAADRTS
                     VYTGARVQANAVVKDQFDQPMNVGVTWSVTPSALGTVSSTGVYVASETATGVASVTAS
                     AAGLSSSVAISVAERPTAFVGGISFQVDASGLADVHTESPIRDTDASTIAVQVDLDVV
                     AIQADYGRAFATRPRLYVLGSTQTYAGALQTILGLDAARAQQLASTSAGLYSPSVNAI
                     AIDWSKVRDIVPLTTARHELTHMMEEQIAGGASMPAWFNEGNARWEELTVAGSQYIAM
                     NGRYATASMAATGTLFSLSDLSSQSTWNARSGLAGTYQYHEAQEAVRL"
BASE COUNT          415 a         1019 c         1007 g          430 t
ORIGIN      
        1 ggctctcgtc gcggcgttcc gagcgaccct cgaggagctc gacgacgccg acctcctcct
       61 gcacgtcgtc gacgccgggc atccgaacct gcacgagcgg ctcatcgtgg tgaaggagac
      121 gctcgggacg ctcggcctcg ccgatcggcc gagcctcctc gtgttcaaca aggccgacac
      181 gctcgccggc gcggagggcg gcgccctccg cgaagcgctc gggggcgagt tcccgggcgc
      241 ggtgttcacc gcggcccgca cgggagcggg cctcgacgcg ctccgcgccc gcctcgccga
      301 gctcgccgcg gccgggtggc agcacgtgaa ggtcgtcgtg ccctatcggg acggcgccct
      361 cgtgcagcgc gtgcgcgagc gcggcgcgct tcgcggcacg cagtacggcg agagcggtat
      421 cgagatcgaa gcagatgtgc cgccggatct ggccagtgag ctgacgcaca gacactcggc
      481 ctgaatcgca ccggcatact tgaggcgagt ggggaagact ctcgtccgcg tcgcgctcgc
      541 ggcgttcctg gcgatctcgt cagttccctt cgtcagccgg ccgtccgtcg cggcgtcctg
      601 cgcggcgacg ggcgtcccga cgcggacgat ctatctgccg aacatcacca agacgctcgg
      661 tggacccgcc ggctgggtga cgccgttcat cgtgcagaac gtcggcgtgg cccccacgga
      721 tctcgagatc tccttctatc ggttcagcga cggcggcctc gtcacgtgcc gccggatcag
      781 cggtctcgca ccgtaccgga gcttcgcgga cgtgccgaac aacgacgtcg acctgcctgc
      841 cgactcgcag ttcgcggtcg tcgtgcgctc ctacggcgcc gaggtcatct cggtcgtgaa
      901 cgaacatcag ggcgccggcg cgacggcgga ggcgctgtcg tacgtcgggc tcgcgagagg
      961 cgcgcagaca caggcgctgc cctacgtcgc caagttcgcc tccggctggc tcgtgacctt
     1021 cgtgatgcag aacctcggtg tcgcgaacgc caatgtcacc gcgcgtttcg tgagcttcga
     1081 cggctccaag accgccacgc tcacgcgcgt catcgcgccc ggtcggtcgc agttcgtgaa
     1141 tcccgtcgcc gagccttcgc tcgcgacggg aacggaatac tccgtgatgc tgacgtccga
     1201 tcagccgatc gccgcgatcg cgaacgcgca caacgatctg ccgggcagcg ccgcgccgat
     1261 gggcttcagc tacaacgccg tcgcgtcgct ctcgacggag gtcatgtacg tgccctcggt
     1321 cgcgcgcaac gcggacggtg tcggacgcac cacacgggtg gtcgtgcaga acacgggcgc
     1381 gttcgacgcg gcgccgacgc tcgtgttccg acggccgggc gcgccgtcgg tgaccgtgaa
     1441 cgtgccgacg gcgatccgtg ccgggcggtc ctgggcgttc gatccgcgcc tcttcgccga
     1501 cggcatcacg ccgtgcccgg ccacgggagc cgcgaactgc gtcggcgagg gcgattggtc
     1561 gctcaccgtg agcggcggcc agttcgccgt gctcgcgatc gcgaccacgt ccgcgacggc
     1621 actcggctcc gtcgggatcg ccgcccccgg taaccgcgcg tatctcccga acgtcacccg
     1681 gaccctcggc ggcgggcttg gatggacgac gccgatcgtc ctgcaatccg ccggcgccac
     1741 gagcgcgacg ctgcgctggt accggttcgt cgacggggtg ctggtcctgc gccagaccgt
     1801 cgcgctctcg aacgggctcg cgatccggat cgacccccgc gccgttggcg gcctcgccga
     1861 cgacacgcag tacgcggtcg tcgtggacgc ccagggtggc gacgtcgcgg cgtcggtcgt
     1921 cgagctgtcg ttcctcggtg gcgacggtgc gatggcgtac gagggcttcg gcgcgaccgt
     1981 cgccacgacc ccgaccgcga cgatgatcag cctcgccgcc gataggacca gcgtctacac
     2041 gggcgcgcgc gtccaggcga acgcggtggt caaggaccag ttcgaccagc cgatgaacgt
     2101 cggcgtgaca tggtccgtca cgccgtcggc gctcggcacg gtcagctcga caggtgtcta
     2161 cgtggcgtcg gagaccgcga cgggtgtcgc gagtgtcacc gcgtcggcgg cgggcctgag
     2221 ctcgagcgtc gccatcagcg tggccgaacg tccgacggcg ttcgttggcg gcatctcctt
     2281 ccaggtcgat gcgtcggggc tcgcggacgt gcacaccgag agcccgatcc gcgacaccga
     2341 cgcgagcacc atcgcggtgc aggtcgacct cgacgtcgtc gcgatccagg cggattacgg
     2401 ccgcgcgttc gcgacacggc ctcgcctcta cgtcctgggc agcacgcaga cgtacgccgg
     2461 cgcgctgcag acgatcctcg gcctcgacgc cgccagggcg cagcaactgg catcgacgag
     2521 cgccggactg tactcgccat cggtgaacgc gatcgcgatc gactggtcga aggtgcgcga
     2581 catcgtgccg ctcacgacgg cgcgccacga gctcacgcac atgatggaag agcagatcgc
     2641 cggcggggcg tcgatgccgg catggttcaa cgaggggaac gcgcgctggg aggagctgac
     2701 cgtcgcgggc agtcagtaca tcgcgatgaa cggccgctac gccaccgcgt cgatggccgc
     2761 gacgggcacg ctgttctcgc tgagcgacct ctcgtcgcag tcgacgtgga atgcgcggtc
     2821 ggggctcgcg ggcacctacc agtatcacga ggcacaggag gccgtccgac t
//
LOCUS       DASTZR010000505         3056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_861191, whole genome shotgun sequence.
ACCESSION   DASTZR010000505 DASTZR010000000
VERSION     DASTZR010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861191"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VFV20_11165"
     CDS             complement(<1..248)
                     /locus_tag="VFV20_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5288952.1"
                     /translation="MPAIPLPPEPQTIEQTGLTVGFLADLALKTLYLRGQMTMGEIAS
                     ALGLAITGVTDRVMEFLKGERLVEIRGGTGISSASYQFV"
     gene            complement(365..2398)
                     /gene="ligA"
                     /locus_tag="VFV20_11170"
     CDS             complement(365..2398)
                     /gene="ligA"
                     /locus_tag="VFV20_11170"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU5288953.1"
                     /translation="MVTSSVERRVADLRERIEKANYEYHVLDQPTMSDEAYDALLREL
                     QELEAKHPELVTPESPTQRVGAAPSTRFAPVAHARPMLSLSNVFDEAELRAFDQRVRK
                     GLGREDVGYVCELKIDGLAINLTYQDGVFVQGATRGDGFTGEDVTANLRTIKSIPLAL
                     RQRVPGRVDVRGEVYLPTASFDTTNRERAERGEPLFANPRNAAAGAVRQLDPAATAKR
                     NLAMFAYSAAGLDVAGQAALLERLKELGVRTNPNWRRAKHVDEVLAFVHEWREKRHGL
                     NYGTDGVVVKVDGAADQEKLGFVARNPRWATAFKFPAEQATTSVEDILVYVGRTGVLT
                     PVAALAPVLVGGTTVRRATLHNLDEVRRLDVRRGDRVVIQRAGDVIPEVVRVEHDART
                     AGDRSGEFEMPATCPVCGGPVEHLEGEVAYRCANTACPAKIGQRIGHFVGRGGMDIEG
                     MGWALVTQLLERGYVTDPADVFFLTKEQLLGLDRFAEKSASNIYERIQAARRRPLARV
                     LNALGIRHVGGTTAEDLARWLAARLPAEPTFAEVLAALRAASAEELQAIPGVGAIVAR
                     AIYDHVRDPEEQRFLERLAEAGVAVTLPAPRPVDAASPLAGKTIVFTGTLERRSREEA
                     EALAKSLGAKVTGSVSAKTDLVVAGAGAGTKLDKARQLGVKVVDEETFDAMLR"
     gene            complement(2422..2688)
                     /locus_tag="VFV20_11175"
     CDS             complement(2422..2688)
                     /locus_tag="VFV20_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288954.1"
                     /translation="MAKTRAERARGRGETPSQRSVRGAQKTGRGDQEVGVTSRARKRI
                     QGGETVADPRAGGRPGRPKTKWRTSAERTSPRQKRAATRKRSTP"
     gene            2838..>3056
                     /locus_tag="VFV20_11180"
     CDS             2838..>3056
                     /locus_tag="VFV20_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288955.1"
                     /translation="MAVIVAPAIRGLTEREAVARAARGERNVLPPATGRTYGEILREN
                     AFTFVNLAILAIGVALALLGEPSDALVSV"
BASE COUNT          398 a         1122 c         1071 g          465 t
ORIGIN      
        1 acgaactgat aggacgcgga cgagatcccc gtgccgccgc ggatctccac cagacgctcg
       61 cccttgagga actccatgac gcggtcggtc acgccggtga tcgcgaggcc gagcgcggag
      121 gcgatctcgc ccatggtcat ctgaccgcgg aggtacaagg tcttcagcgc gagatcggcg
      181 aggaagccga cggtgagccc cgtctgctcg atggtctgcg gctcaggcgg gagcgggatg
      241 gcgggcatgt cggcgggtgc cgcggctcct gcgggcgcgg ccggacgagg cgcagtctgt
      301 ggctggtaca cgcttcctct ttacccttcc tcgcgctcgc ttccggctat tcgttcgata
      361 tttgctaacg gagcatagcg tcgaaggtct cctcgtccac cactttgaca ccgagctgac
      421 gcgccttgtc gagcttcgtg ccggcccccg cgccggccac gacgagatcc gtcttcgcgc
      481 tcacggaacc ggtgaccttc gcgcccagtg acttcgcgag cgcctcggcc tcttcgcgcg
      541 agcggcgctc cagcgtcccg gtgaagacga tggtcttgcc cgcgagcgga ctcgcggcat
      601 cgactggacg cggcgcgggc agcgtgaccg cgacgccggc ctcggcgaga cgctcgagga
      661 agcgctgctc ctcgggatcg cggacgtggt cgtagatggc gcgcgcgacg atcgcgccga
      721 ctccggggat cgcctgcagc tcctccgcgc tcgccgcacg cagcgccgcg aggacctccg
      781 cgaacgtggg ctcggccggc aggcgcgcgg cgagccagcg cgcgagatcc tccgcggtgg
      841 tcccgccgac gtggcggatg ccgagcgcgt tgagcacgcg cgcgaggggt cgacggcgcg
      901 cggcctggat gcgctcgtag atgttcgacg cgctcttctc ggcgaagcga tcgagcccga
      961 gcagctgctc cttcgtgagg aagaacacgt ccgcgggatc ggtgacgtag ccccgttcga
     1021 gcagctgcgt caccagcgcc cagcccatgc cttcgatgtc catgccgccg cgcccgacga
     1081 agtggccgat gcgctggccg atcttcgcgg ggcaggctgt attcgcgcag cggtacgcga
     1141 cctcgccctc gaggtgctcg accggcccgc cacagaccgg acacgtggct ggcatctcga
     1201 actccccaga gcggtccccc gccgtgcgcg cgtcgtgctc gacgcgcacg acctccggga
     1261 tgacgtcgcc ggcgcgctgg atcacgacgc ggtcgcccct gcgcacgtcc aggcgccgca
     1321 cctcgtcgag gttgtgcagc gtcgcgcggc gcaccgtggt gccgccgacc agcacgggcg
     1381 cgagcgcggc gaccggggtc agcacgccgg tccggccgac gtacacgagg atgtcctcca
     1441 cgctcgtcgt ggcctgctcc gccgggaact tgaacgccgt cgcccagcgc gggttccgcg
     1501 cgacgaagcc gagcttctcc tgatcggcag cgccgtcgac cttcaccacc acgccgtcgg
     1561 tgccgtagtt caggccgtgc cgcttctccc tccattcgtg cacgaacgcg aggacctcgt
     1621 cgacgtgctt cgcgcgccgc cagttcgggt tggtgcgcac gccaagctcc ttgaggcgct
     1681 cgagcagcgc cgcctgtccg gccacatcga ggcccgcggc ggagtacgcg aacatcgcga
     1741 ggtttcgctt cgcggtcgcg gccggatcga gctggcgcac cgcaccggcg gcggcgttgc
     1801 gcgggttcgc gaagagcggc tcgccgcgct cggcgcgctc gcggttcgtc gtgtcgaacg
     1861 acgcggtcgg caggtagacc tcaccgcgca cgtccacgcg ccccgggacg cgctgccgca
     1921 gcgcgagcgg gatggacttg atggtgcgga gattcgcggt gacgtcctcg ccggtgaagc
     1981 cgtcgccgcg cgtcgcaccc tgcacgaaca cgccgtcctg gtacgtcaga ttgatcgcca
     2041 ggccgtcgat cttcagctcg cagacgtagc ccacgtcctc gcgccccaga cctttccgca
     2101 cgcgctgatc gaacgcgcgc agctccgcct catcgaagac gttcgagagg ctgagcatcg
     2161 gccgcgcgtg cgccaccggc gcgaagcgcg tcgacggcgc cgcgccgacg cgctgggtcg
     2221 gcgactccgg cgtcacgagc tccggatgct tcgcctcgag ctcctgcagc tcccgcagga
     2281 gcgcgtcgta cgcctcgtcg gacatcgtcg gctgatcgag gacgtggtac tcgtagttcg
     2341 ccttctcgat gcgctcgcgc aggtcggcga cgcggcgctc cacggacgag gtgaccatcg
     2401 cgcacggatt gtcgcgcagg cctagggcgt ggagcgcttc ctcgtggccg cgcgcttctg
     2461 gcgcggcgag gtgcgctcgg cggacgtgcg ccatttcgtc ttcgggcgcc cgggccggcc
     2521 accggcgcgc gggtcggcga cggtctcgcc accctggatg cgctttcgcg cccggctcgt
     2581 gacgccgacc tcctggtcgc cgcgtcccgt cttctgcgca ccacgcaccg accgctgtga
     2641 gggcgtctca ccgcggcccc tggctcgctc ggctcgcgtc ttcgccatct cggacctcct
     2701 cgcgtgctca cgagcgtacg tcgcgggccc cgtgtgcggc tgcgcccgac acggccgcgg
     2761 caggagcgtg accacgcgcg gagaggacgg gaccatcggc cgtcggcacg gccgccgatc
     2821 gagccgacca tgacggcatg gcggtcatcg tggcacccgc gatccgcgga ctcaccgagc
     2881 gcgaagcggt cgcgcgcgcc gcgcgcggcg agcggaacgt gctgccgccg gcgacgggac
     2941 ggacgtacgg cgagatcctg cgcgagaacg cgttcacctt cgtcaatctc gcgatcctcg
     3001 cgatcggcgt cgcgctcgcg ctcctgggcg agccgagcga cgccctcgtc tcggtc
//
LOCUS       DASTZR010000506         5640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_861643, whole genome shotgun sequence.
ACCESSION   DASTZR010000506 DASTZR010000000
VERSION     DASTZR010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861643"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VFV20_11185"
     CDS             <1..604
                     /locus_tag="VFV20_11185"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HEU5288956.1"
                     /translation="PRLFRRFIAEWPNIELQIVEGASDDELLRSVEHGDLDVTFCVLP
                     LAEGPFEAAELMSDPYVLAVPAGSPLAGSRRATLGRLRGVKLVGFRLDRTVIAVEDLL
                     RTHGVEPNVVFRSNDNGIVQGMVGAGLGMALVPRLALDEQDPAIRLVGLPEMPPRVLA
                     MAWHHDRYRSPAALAIVDHARAVCRELKAVPRIRKASPRQ"
     gene            complement(655..1356)
                     /locus_tag="VFV20_11190"
     CDS             complement(655..1356)
                     /locus_tag="VFV20_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5288957.1"
                     /translation="MSGDGARVLIVDDEPAILRTVRANLGRRGFLVDTATSGTEALSR
                     LDTRPDLVVLDLGLPDRDGLDVIRAIRQRSDTPIIVLSVRDAEREKVRALELGADDYL
                     TKPFGVDELVARVRVALRHGARLPPGPVFSAGDLTVDLDRRRVLVSGDEVRLTPTEYG
                     LLAALVRHADHVMTDASLLQQVWGPEYGDEDHYLHVYVARLRKKIERDPQKPRFLITE
                     PGVGYRLLTEPRAGV"
     gene            complement(1353..2486)
                     /locus_tag="VFV20_11195"
     CDS             complement(1353..2486)
                     /locus_tag="VFV20_11195"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5288958.1"
                     /translation="MRGLGIAAYVSTLAAVALCTAVIGVIEARGHISNISMLYLLVVL
                     ASAILAGRRAAIAASVAAFLAFNWFFVQPLHTFQVGDPDEWLALLVFLLTAGITGQLA
                     AQLRDRARVAAARERETVQLYEERERLRQQATNAEVLRRADELKTALLGAVSHDLRTP
                     LASIIASAGSLRQTDVEWTPAEREAFVADIEGEARRLERIVANLLDLSRMESGTLRPE
                     RGWYDVAALVDDVLGRLRTITARHAVHVDISDDLPPVPLDYVEIDQVISNLIENAVRH
                     TPAGTNVWITAERAGESVAVSVADDGPGVPAELQGRLFDAFARAGDRRGMQRGAGLGL
                     AIARGLISAHGGTIAADARPGGGTVVRFTLPLRVSARVEAATT"
     gene            2687..4636
                     /locus_tag="VFV20_11200"
     CDS             2687..4636
                     /locus_tag="VFV20_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU5288959.1"
                     /translation="MRIVRPAEFRRSGGGFVATEKALEVSGGTLDRAWMSVRRALIGR
                     RLATEEEHLERVSKKIGLAIFASDNVSSSAYATEETMRVLALAGTGALALTMPLTLAI
                     VVVLAIVVLSYLQVIRAYPQGGGSYVVASENLGIVPGLVAASALLVDYFLTVAVSVAA
                     GVAAITSAFPALHPERVVISVTLVALLTVGNLRGIREAGLIFAAPTYLYVASILGLLA
                     IGLWSVVTGAPPRPAPPIQAFVPEGTQALTLLLVLRAFASGSVALTGTEAVSNGVQAF
                     KAPADRNASAVLVIMGALFATLFVAISFLATTIGIAPDASETETVLSLLARSLVGTSW
                     YFYLVQATTAVILILAANTAFNGFPRLASIMATDRFMPRQFAQRGDRLAFSTGIVALA
                     LVSSVLIVAYDASVTGLIPLYTVGVFIAFTLSQAGMVRRWVRLRSRRWFLSAAVNGAG
                     AIATGAVALVVGASKFQLGAWMVVAIIPVIVAILYGIGMHYRTVGDRLLIAASAPVRL
                     IPSRPRVVVPISRIDRPALRALALATGLGGDVFAVHISFDSEGARAFQRRWAEVVGDA
                     VPLDTVISPYRALQAPLLRYIDALDHDPPRPTIVVLAEFVPRHWWEALLHNQTALLLK
                     LRLFVRRNTSVLDVPYHLDEADKEE"
     gene            complement(4710..5294)
                     /locus_tag="VFV20_11205"
     CDS             complement(4710..5294)
                     /locus_tag="VFV20_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288960.1"
                     /translation="MRVSILAILALLAITFASAAPASAAGVETGTSGVFSIGPGHTFV
                     ADVPGATSTLERTPNGVSVSVRTSGLLAGHAVTVWALIFNDPTACLAGCEEMRGDLNV
                     QSVKGSVFHVTGHVVAAGETTFGGRVNVGDSAHAFVGPGLLDPYGARIHLIIRDHGVA
                     GTGELLQQQFNDTSPRFCNVACFDAQKSVHLPHR"
BASE COUNT          825 a         1956 c         1943 g          916 t
ORIGIN      
        1 gccccgccta ttccgccgct tcatcgcgga gtggccgaac atcgagcttc aaatcgtcga
       61 aggagccagc gacgacgagc ttctgcggag cgtggaacac ggcgacctcg acgtcacgtt
      121 ctgcgtgctt ccgcttgcgg agggaccgtt cgaggccgca gagctgatga gcgatcccta
      181 tgtgctcgct gtgcccgcgg gctcgcccct cgcgggatcg cgacgagcca ccctcgggcg
      241 gctgcgaggc gtgaagctcg ttgggttcag gctcgatcgg accgtcatcg cggtcgagga
      301 tctcctgcga acccacggcg tcgagccgaa cgtcgtcttc cgatcgaacg acaacggcat
      361 cgttcagggg atggtggggg ccggtctggg aatggccctc gtgcctcggc tcgcgctcga
      421 cgagcaggat cccgcgatcc gcctcgtcgg gcttcccgag atgccgccga gagtgctggc
      481 gatggcgtgg caccacgacc gctatcgatc gccggccgct ctcgcgatcg tcgaccatgc
      541 gcgtgccgtg tgccgagaac tgaaggccgt gccccgtatc cggaaggcat cgcctcggca
      601 gtagtgactt cgtaaattcc acgcgcgttc cgcgcacgat ccgcggactg gtcttcagac
      661 cccagcacgc ggctcggtca acagccggta gccgacgccc ggctcggtaa tgaggaaccg
      721 cggcttctgc ggatcgcgct cgatcttctt tcgcaggcgc gccacgtaga cgtggaggta
      781 gtggtcctcg tcgccgtact cgggccccca cacctgctgc aacaggctcg cgtccgtcat
      841 cacgtggtcc gcatggcgca cgagcgcggc gagcagcccg tactccgtcg gtgtcagacg
      901 cacctcatcg cccgagacaa gcacccgtcg tcgatcgagg tcgacggtga gatcgcccgc
      961 actgaagacc ggcccgggcg gcaggcgcgc gccgtggcgc agcgcgacgc gcacgcgggc
     1021 gacaagctcg tcgacgccga acggcttggt gaggtagtcg tccgcgccga gctcgagcgc
     1081 gcgcaccttc tcgcgctcgg catcccggac cgacagcacg atgatcgggg tgtccgaacg
     1141 ctggcggatc gcgcggatca cgtcgaggcc gtcgcgatcc gggaggccga gatcgagcac
     1201 gaccaggtcc ggtcgcgtgt cgagccggga cagcgcctcg gtgccggacg tcgccgtgtc
     1261 gacgaggaaa ccccgtcggc cgaggttggc gcggaccgta cggaggatcg cgggctcatc
     1321 gtcgacgatg agcacccggg cgccgtcgcc gctcacgtcg tggccgcctc cacgcgcgcg
     1381 ctgacgcgca gcggcagcgt gaagcgcacg accgtgcctc cgccgggtcg cgcgtccgcg
     1441 gcgatcgtgc ctccgtgcgc gctgatgagt ccgcgcgcga tcgcgagtcc gagaccggcg
     1501 ccgcgctgca tgccgcggcg gtcgcccgcg cgtgcgaacg cgtcgaagag gcgcccctga
     1561 agctcagcgg gcacgccggg cccgtcgtcc gcgaccgaga ccgcgacgct ttcgcccgcg
     1621 cgctccgccg tgatccacac gttcgtcccg gcgggtgtgt gccgcacggc gttctcgatg
     1681 agattcgaga tgacctgatc gatctcgacg tagtcgagcg gtacgggcgg caggtcgtcg
     1741 gagatgtcga cgtgcacagc gtgccgggcc gtgatcgtgc gcaggcgtcc gaggacatca
     1801 tcgaccagcg cggcgacgtc gtaccacccg cgctccgggc gcagtgttcc cgactccatc
     1861 cgtgagaggt cgagaagatt ggcgacaatg cgctcgaggc gccgggcctc gccctcgatg
     1921 tccgccacga acgcctcgcg ctccgccggc gtccactcga cgtcggtctg gcggaggctt
     1981 cccgcgctcg cgatgatcga ggcgagcggc gtgcgcagat cgtgcgagac cgcgccgagg
     2041 agcgcggtct tgagctcgtc ggcacgccgg agcacttcgg cgttcgtcgc ctgctgccgc
     2101 agccgctcgc gctcctcgta cagctgtacg gtctcgcgct cgcgcgcggc cgcgacacgt
     2161 gcccgatcgc gcagctgcgc cgcgagctgc ccggtgatgc ccgcggtgag caggaagacg
     2221 aggagcgcca gccactcgtc gggatcgccg acctggaagg tgtgaagcgg ctggacgaag
     2281 aaccagttga acgcgaggaa ggccgcgacc gatgccgcga tcgccgcgcg gcgtcctgcg
     2341 aggatcgccg acgcgagaac gacgaggagg tagagcatcg agatgttcga gatgtggcca
     2401 cgcgcttcga ttacgccgat gaccgcggtg cacagggcga ccgccgcgag cgtcgagacg
     2461 tatgccgcga tgccgagccc gcgcatgtat cgaggatagg cgtcgaacgc gcgtgtggag
     2521 cacggcctca acagatcctc aacggcccgc atgcgttcct agactcggat gagtgaccga
     2581 cacacgagag ccgaacacga gatcgcagtc agacagcagc cctgctcatc acggcgccct
     2641 ggagcgtcgc gaggagctgc gtggtcgacg gcccggtgac cggcgcgtgc gcatcgtccg
     2701 gcccgccgag ttccgacggt ccggcggcgg cttcgtggcc acggagaagg cgctcgaggt
     2761 gtcgggcggt accctcgatc gtgcgtggat gtcggtgcgc cgagcgctca tcggacgccg
     2821 gctcgcgacc gaagaagaac acctcgaacg cgtttccaag aagatcggtc ttgcgatctt
     2881 cgcctccgac aacgtctcgt cctccgcata cgccaccgag gagacgatgc gcgtcctcgc
     2941 gctcgccggg accggcgcgc tcgcgctcac gatgccgctc acgctcgcga tcgtggtcgt
     3001 cctcgcgatc gtcgtcctct cgtatctgca ggtgatccgc gcatacccgc aggggggagg
     3061 aagctacgtc gtcgcgagcg agaacctggg gatcgttccc gggctggtcg ccgcgagcgc
     3121 tctgctggtg gactacttcc tgacggtggc cgtctccgtc gccgcgggcg tggccgcgat
     3181 cacgagcgcg ttccccgcgc tccaccccga gcgcgtggtc atctcggtga cgctcgtcgc
     3241 cctcctcacc gtgggcaacc tgcgtggcat acgcgaggcg ggcctcatct tcgcggcgcc
     3301 gacgtatctc tacgtcgcgt ccatcctcgg cctcctagcc atcgggctgt ggagcgtcgt
     3361 gacgggagcg cctccgcgac cggcgccgcc gatccaggcg ttcgtgcccg agggcaccca
     3421 ggccctcacg ctgctgctcg tgctgcgcgc gttcgcctcg ggatcggtcg cgctcaccgg
     3481 caccgaggcg gtgtcgaatg gcgtgcaggc gttcaaggca cccgccgacc gcaacgcgag
     3541 cgccgtgctc gtcatcatgg gcgcgctctt cgcgacgctg ttcgtggcga tcagcttcct
     3601 tgcgacgacc atcggcatcg cacccgacgc gagcgagacc gagacggtgc tcagcctgct
     3661 cgcgaggtcg ctcgtcggca cctcgtggta cttctacctc gttcaggcga cgacggcggt
     3721 catcctgatc cttgccgcga acacggcctt caatggcttt ccgcgtctgg cctcgatcat
     3781 ggcgaccgat cgcttcatgc cacgccagtt cgcacagcgt ggcgatcgcc tcgcgttcag
     3841 caccgggatc gtcgccctcg ccctcgtgtc ctcggtgctg atcgtggcct acgacgcgag
     3901 cgtgaccggg ctcatcccgc tgtacaccgt gggcgtcttc atcgcgttca cgctgtcgca
     3961 ggcggggatg gtgcgtcgct gggtgcgact gcgctcgcgc cgctggttcc tctccgcggc
     4021 ggtcaacggc gccggtgcga tcgcgaccgg tgccgtcgcg ctcgtggtcg gcgcgagcaa
     4081 gttccagctc ggtgcgtgga tggtggtcgc gatcatcccc gtcatcgtcg cgatcctcta
     4141 tgggatcggc atgcactacc gcacggtcgg cgatcgcctg ctgatcgcgg ccagcgctcc
     4201 ggtgcgcctc ataccatcca ggccccgcgt cgtcgtgccg atcagccgga tcgaccgacc
     4261 ggccctgcgc gcactggcgc tcgccaccgg actgggtggc gacgtattcg cggtccacat
     4321 ctcgttcgac agcgagggtg cgcgagcctt ccaacgacgc tgggcagagg tggtcggcga
     4381 cgcggtcccg ctcgatacgg tcatctcgcc gtaccgcgcc cttcaggcac cgctcctccg
     4441 gtacatcgat gctctcgatc acgatccgcc gcgaccgacc atcgtggttc tcgcggagtt
     4501 cgtgcctcgt cactggtggg aggcgctgct gcacaaccag accgcgctgc tgctgaagct
     4561 gcggctcttc gtgcggcgca acacgtcggt gctcgacgtc ccctatcacc tcgacgaggc
     4621 ggataaggag gagtgaagac gacgcgacgc gcatagccgg cgcgcggtcc ctcgatgacc
     4681 gtgcgccggc catgactcaa ttgcctcggt caccgatgcg gcaaatgcac cgacttctgc
     4741 gcgtcgaagc acgcgacgtt gcagaaccgc ggcgaggtgt cgttgaactg ctgctgcaag
     4801 agctccccgg tccccgctac gccgtggtcg cggatgatga ggtggatccg cgcgccatac
     4861 ggatcgagca ggccgggccc cacgaatgcg tgtgcgctgt cgccgacgtt cacacggcca
     4921 ccgaaggtgg tctcacccgc ggcgacgacg tgtccggtga cgtggaagac ggaccccttg
     4981 acggactgaa cgttcaggtc gccgcgcatc tcctcgcagc cggcgaggca cgcggtcgga
     5041 tcgttgaaga tgagagccca caccgtcacg gcgtgccccg caagcagacc tgacgtccgg
     5101 accgagacgc tgaccccatt cggcgtgcgc tcgagtgtcg aggtcgcgcc gggcacgtcg
     5161 gcgacgaacg tgtgcccggg gccgatcgag aacacgccgc tcgtgccagt ctcgacgccg
     5221 gcggcggatg cgggcgcggc ggaggcgaac gtgatcgcga ggagagcgag gatcgcgagg
     5281 atcgatacgc gcatgtctgt tcctccctgt tgccgccgtc cgccagtcgc ggttcgcagc
     5341 ggtctgctcc acgaacggtc ccgcctcggc ggcgatttcg cctcctgtga tgcccgtcac
     5401 atgtcgcgtg atcttcccgt gatcacctgg ccggcgccga acgtcctggg atgttgcccc
     5461 ttccgggagt gcgcaggaga cccgactgcg tgtgcgcagc gggtctcccg cggggaaggg
     5521 tgtgggatga ggatcaggcg gtgcggtgct gttgcgtgcc ggcgtgtgcg tggacgctcg
     5581 ctggtgccgt gcgccacacg agccaccatg ccaggacgag tccgtacatg gcgtgcgcga
//
LOCUS       DASTZR010000507         2202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_417555, whole genome shotgun sequence.
ACCESSION   DASTZR010000507 DASTZR010000000
VERSION     DASTZR010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417555"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..475
                     /locus_tag="VFV20_11210"
     CDS             77..475
                     /locus_tag="VFV20_11210"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU5288961.1"
                     /translation="MERVAQTWRYLRVARGDDRHAQRTADILRSLGADDELVLAGLLH
                     DRAKPAETRLWHRIAAVMLETLAPPLRTRLARGSGVFARYLDHATLGAALAKAEGRSE
                     RVVRLIARHHERPRDPDETLLARADREAIP"
     gene            475..1215
                     /locus_tag="VFV20_11215"
     CDS             475..1215
                     /locus_tag="VFV20_11215"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEU5288962.1"
                     /translation="MLGHEGPNVSVSGFDGPLELLLQLVEEKKLDILTVRLGDLADAY
                     LVRVRALDTLPADEVASFLALASRLVLLKARSLLPAFAPIADEEEGESEEELRQRLLE
                     YAVVRERAGALGDRLRSGQRAFHHEGGSSADLPPRGGEVTALAAAWSRVLALAARQRE
                     EDMVSPGERYSVEERTAEIETIVTERGAITFSELLGAAPTLGWAVVTFIALLDLYRRI
                     IIDLQQDTLFTDILITKKERPAVTETPT"
     gene            1235..1786
                     /gene="scpB"
                     /locus_tag="VFV20_11220"
     CDS             1235..1786
                     /gene="scpB"
                     /locus_tag="VFV20_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEU5288963.1"
                     /translation="MRSPLANAVEAVLFVVEKPVSRGEIGKVVGATPKQVDTALLELA
                     EGYAGTGLRLAHDGDDWQLVTAPEQATAVAAYLGADRLRVSPASLETLAVIAYRQPVT
                     RAEVEAIRGVNCDQTIYTLMQRRLIEERGRREGPGRPILYGTTWEFLECFGLSSLDDL
                     PRALTPEGGVVGQPGSVEDATRP"
     gene            1783..>2202
                     /locus_tag="VFV20_11225"
     CDS             1783..>2202
                     /locus_tag="VFV20_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011667545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEU5288964.1"
                     /translation="MIRLQKVLADRGVASRRAAEELITSGRVRVNGAIVTTLGTKVAP
                     DARIEVDGATTHVAAARYLAMNKPKGIVSTAHDERGRRTVVDLVDVSERLYPVGRLDA
                     DSEGLLLLTNDGDWAEHVLHPRYGHEREYEVTVTGELS"
BASE COUNT          327 a          798 c          777 g          300 t
ORIGIN      
        1 cgcggatctt caccgcgatc gtgatgcagc cggccctcgc tctcacgacg ctcctgaccg
       61 gactgcgttg agattgatgg aacgcgtcgc gcagacgtgg cgctacctca gggtggcccg
      121 cggggacgac cggcacgccc agcggaccgc cgacatcctg cgctcgctgg gcgccgacga
      181 cgagctcgtg ctcgcgggcc tgctgcacga ccgcgcgaag cccgccgaga cgcggctctg
      241 gcatcgcatc gccgccgtga tgctcgagac cctcgcgccg cccctccgca cgcgcctcgc
      301 tcgcgggagc ggcgtattcg cgcgatacct cgatcacgcc acgctcggtg ccgcgctcgc
      361 gaaggccgag ggccgctcgg agcgcgtcgt ccggctcatc gcgcgccatc acgagcgtcc
      421 gcgcgacccc gacgagacgc tcctcgcacg cgccgaccgc gaggccatcc cctgatgctc
      481 ggccacgagg gcccgaacgt cagcgtgagc ggtttcgacg gcccgctcga gcttctgctc
      541 cagctcgtcg aggagaagaa gctcgacatc ctcaccgtcc ggctcggcga tctcgcggac
      601 gcctatctcg tacgcgtgcg cgcgctcgac acgctgcccg ccgacgaggt cgcgtcgttc
      661 ctcgcgctcg catcccgcct cgtcctgctg aaggcgcgga gcctgcttcc ggcgttcgcg
      721 ccgatcgcgg acgaggagga aggcgagtcc gaggaggagc tgcgccagcg cctcctcgag
      781 tacgcggtcg tccgcgagcg cgcgggtgcg ctgggcgatc gcctccggag cggccaacgt
      841 gcgttccacc acgagggcgg atcgtccgcc gacctgccgc cgcgcggcgg agaggtcacg
      901 gcgctcgcgg cggcatggtc gcgggtcctc gcgctcgccg cccgccagcg cgaggaggac
      961 atggtgtcgc cgggagagcg ctactcggtc gaggagcgca cggcggagat cgagacgatc
     1021 gtgaccgagc gcggcgcgat cacgttcagc gagctcctcg gtgcggctcc cacgctgggc
     1081 tgggctgtcg taacgttcat cgcgctgctg gacctgtatc gccgcatcat catcgacctc
     1141 cagcaggaca ccctgttcac cgacatcctg atcacgaaga aggagcggcc cgccgtgacg
     1201 gagacgccca catgagagcg cgagcgcgac gatcatgaga tcaccgctgg ccaacgccgt
     1261 cgaggcggtg ctgttcgtcg tggagaagcc ggtctcccgc ggggagatcg gcaaggtcgt
     1321 gggagcgacg ccgaagcagg tcgataccgc gctcctcgag ctcgcggagg ggtacgccgg
     1381 cacgggcctg cgcctcgcgc acgacggcga tgactggcag ctcgtgaccg ctcccgagca
     1441 ggcgaccgcg gtggccgcgt acctcggcgc cgaccggctg cgggtgtccc cggcgtcgct
     1501 cgagacgctc gccgtgatcg cgtaccgcca acctgtcacg cgtgcggagg tcgaggcgat
     1561 ccgcggcgtg aactgcgacc agacgatcta cacgctcatg cagcggcgcc tcatcgaaga
     1621 acgtggccgc cgcgaagggc cgggccgccc gatcctgtac ggcacgacct gggagttcct
     1681 cgaatgcttc ggcctctcga gcctcgacga cctgccgcgg gcgctcacgc ccgagggcgg
     1741 cgtcgtcggc cagccgggca gcgtggagga cgcgacccgg ccgtgatccg tctgcagaag
     1801 gtgctggcgg accgcggcgt cgcgtcccgt cgcgccgccg aagagctcat cacctcgggc
     1861 cgggtacggg tgaacggcgc catcgtgacg acgctcggca cgaaggtggc gcccgacgcg
     1921 cgcatcgagg tcgatggcgc gacgacgcac gtggccgccg cgcgatacct cgccatgaac
     1981 aagccgaagg ggatcgtttc caccgcgcac gacgagcgcg gacggcgcac ggtcgtggac
     2041 ctcgtcgacg tcagcgagcg cctctacccg gtgggccgcc tggacgccga ttcggaaggc
     2101 ctgctcctgc tcacgaacga cggcgactgg gccgagcacg tgctccatcc gcgttacggg
     2161 cacgagcgcg aatacgaagt gacggtgacc ggcgagctct cg
//
LOCUS       DASTZR010000508         7662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_364922, whole genome shotgun sequence.
ACCESSION   DASTZR010000508 DASTZR010000000
VERSION     DASTZR010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7662
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364922"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VFV20_11230"
     CDS             complement(<1..605)
                     /locus_tag="VFV20_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HEU5288965.1"
                     /translation="MSTERVLFTSESVTEGHPDKLCDQISDAMLDECLKQDPSSRVAC
                     ETATTTGTILVCGEITTKAWVDVPAMVRGVVKDIGYIDAEYGFDYQSCGVLTAIKEQS
                     KEIAQGVDESFEAKAGTDPHELGAGDQGMMFGFAVRETPELMPLPITLAHALARGLAE
                     ARRTRTIPYLRPDGKSQVTVEYDRGVPKRVHTIVVAAQHDPG"
     gene            complement(623..1909)
                     /gene="ahcY"
                     /locus_tag="VFV20_11235"
     CDS             complement(623..1909)
                     /gene="ahcY"
                     /locus_tag="VFV20_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEU5288966.1"
                     /translation="MTTTSATVKTREHDVSDIGLAGAGERRILWAAREMPVLAGIAER
                     FKKERPLAGLRIGACLHVTAETANLMLALKAGGASVALCASNPLSTQDDVAASLVAND
                     GISVYARKGEDRDTYYSHINAVLATKPQMTMDDGADLVTELHTKRRELLPEVTGGTEE
                     TTTGVTRLRAMASEGVLAYPIVAVNDALTKHFFDNRYGTGQSAIDGVIRATNLLLAGK
                     TAVVAGYGWVGRGIASRLRGMGAITIVCEIDPFRALEAAMDGFEVTTMVEAAARGDLF
                     ITATGNVNVIGKDALAKLKDGAIVANAGHFNDEIDLATLTTLASGCRELRPFVNEYRL
                     RDGRSVVVLADGRLVNLAAAEGHPPAVMDMSFANQALSAEYMVKNAKTLAPQVYPVPR
                     ELDEEIARRKLAAMGMHIDAMSEEQRVYANSWKAGT"
     gene            complement(1906..2733)
                     /locus_tag="VFV20_11240"
     CDS             complement(1906..2733)
                     /locus_tag="VFV20_11240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5288967.1"
                     /translation="MTAAPLLPEIRVDEQVPPERLRAFLERDRLLAAYALADLDATDF
                     ERARWWLARRGDEPVAAALVVEALAFRPCFAIGETEALAAVYREGIRESRLVISTPPA
                     GRLAAEAAYRFERVDRMHRMAVDVQAFNPRVTHRVARLGPEHLEDIIDLYGHASRTYF
                     TPDRLRREIYFGIYVGDALVSAAGTHVRSVRAGLAAVGNVLTKLGHRDRGMATSVTSA
                     VTQAALEDHRDVVLNVRQDNAPAIAVYERLGYHVHAPFIEGPAVRRSALDRILGRMR"
     gene            complement(2836..3363)
                     /locus_tag="VFV20_11245"
     CDS             complement(2836..3363)
                     /locus_tag="VFV20_11245"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEU5288968.1"
                     /translation="MATTVKDLSGRIRDVADFPKPGILFKDITTLLKDGAAFRASIDG
                     LLEKVGKRDVDAVVGMESRGFIFGAPIAYKLGVGFVPVRKLGKLPGDVVSVEYDLEYG
                     SATLEMHRDALAPGAKVLIVDDLLATGGTVAGTIELVKQLRGEIVACAFLIELTALHG
                     RDKLGGYDVVTLISY"
     gene            complement(3373..4404)
                     /locus_tag="VFV20_11250"
     CDS             complement(3373..4404)
                     /locus_tag="VFV20_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288969.1"
                     /translation="MSRFARGVTLALVLAACQTAAPTATPSAASAGIVYGVAGLVRPH
                     APNSTAADAEAMYRSFAETGGALGVYTNWADRADTEGKVPAVVPAAFAAAKQYSFGPL
                     VIALGVAADAGGTGVKSTIDWSSTQRERFLATVTQVARDHRPRFLALGVETNRLWLSD
                     RTSFDGFVRGYADAYDAVKKVSPDTKVFTIFQLELMRGHATRMTGRDDEPQWDLLERF
                     AGRLDLLGLTTYPFLEYQRPSDLPEDYYSDAARRLRAPVAFTEIGWPSARPASAVAAY
                     DASPDDQAEFVARFFALTAGMRPAIALWSFPFELGVPSSLFDSVGIRRTDGAAKPALA
                     AWQRGIRTK"
     gene            complement(4401..4994)
                     /locus_tag="VFV20_11255"
     CDS             complement(4401..4994)
                     /locus_tag="VFV20_11255"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU5288970.1"
                     /translation="MTTFFFVRHGESEANASHRFSGRSDSPLTERGRQQAVAVADALA
                     EVPFDRIVATPLSRSLDTALVIARRRRMPVDVEPGLVEIDVGDRTGAHFDEVRGLPEW
                     KDDGFVSWPGGESLEQVLERGLRAVRRLARETSGGTVLVIGHGGVTRILVSHYLGLLP
                     KLDRSPATNTNVTVIVTDGETGRVERLFESAHVVSRS"
     gene            complement(4994..5614)
                     /locus_tag="VFV20_11260"
     CDS             complement(4994..5614)
                     /locus_tag="VFV20_11260"
                     /inference="COORDINATES: protein motif:HMM:NF018627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288971.1"
                     /translation="MKIGVDLDDVIAVCAVPYLRKFAAEYKVELPDEQEIGWHLLRQM
                     DDKVPARERDRFRMRLYDGTFFSDLDIYEDCPIVLEQLVQRGHEIYFITARNEARRMV
                     TETWLREKRILDYAKAVHLKPHGEFNPDYPRGRYDPQSSAAYKVRLAQELRLDLFCED
                     DVIISEALAEAGVRVLLFDHPWNRDVSHERIIRVNGWSDVAARLGV"
     gene            complement(5676..6347)
                     /locus_tag="VFV20_11265"
     CDS             complement(5676..6347)
                     /locus_tag="VFV20_11265"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEU5288972.1"
                     /translation="MIWIFAALLVIWGNVVNYFVQPALPGGDWAAVSWGAALVAVSLG
                     VARLLRQRRGDLGLVRGDPRGIGAAAAVALVAAAVGTAVLRVGPIVGAPIGYQPLSAT
                     TPDALALHIVLFLPLAAVIPEEVAFRGVLLGGLWTRTGARKAVVGSSAAFALWHAFVV
                     WVTLLQTSLAGTALAWLAGMAALVFVAIGGAVLALLRIRSGGIAAPVVAHWAFDATLL
                     VGLWV"
     gene            complement(6370..>7662)
                     /locus_tag="VFV20_11270"
     CDS             complement(6370..>7662)
                     /locus_tag="VFV20_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HEU5288973.1"
                     /translation="DVVIGTHRLLQKDVRFRDLGLLVIDEEHRFGVKHKERLKQMRTE
                     VHVLSMTATPIPRTLHMALSGIRDISVIETPPEDRQPIETHVVERTDDVLREAILREL
                     DRGGQVYYVHNRVQGIEHETRRLRVLVPNARFVVAHGQMDERKLERVMVEFADAEHDV
                     LVCTTIIESGLDIPNVNTIVIDHAGQLGLAQLYQLRGRVGRAAAKAYAYLLYSKQERL
                     SEDARKRLQAVFEASELGAGFKIAMHDLEIRGAGNILGAEQHGHVAAVGFELYTQMLE
                     EAVNAERGAPQREALPEVTVDLALSTAIPDDYVPSRQRKLELYRRLGELRSTEELSAL
                     RDELRDRYGPPPEPVRNLLYGVEVKLRAVAAGATEVRARGPELRLVLASDLTPDRRGR
                     LAKAFPRVQAGTRQIRLSVLDTRGDWRDALTRLLDLVA"
BASE COUNT         1124 a         2663 c         2720 g         1155 t
ORIGIN      
        1 cccggatcgt gctgtgccgc gaccacgatc gtgtgcacgc gcttcgggac gccgcggtcg
       61 tactcgacgg tgacctgtga cttcccgtcg ggccgcaggt acgggatcgt ccgcgtgcga
      121 cgcgcctcgg cgagcccgcg cgcgagcgcg tgcgcgaggg tgatcggcag cggcatgagc
      181 tccggcgttt cgcggacggc gaagccgaac atcatgccct ggtcgcccgc gccgagctcg
      241 tgggggtcgg tccccgcttt ggcctcgaac gattcgtcga cgccctgcgc gatctccttc
      301 gactgctcct tgatggcggt gagcacgccg caggactggt agtcgaatcc gtactcggcg
      361 tcgatgtatc cgatgtcctt caccacgcca cggaccatgg cgggcacgtc gacccacgcc
      421 ttggtggtga tctcgccgca gacgaggatc gttcccgtcg tcgtggcggt ctcgcacgcc
      481 acgcgcgagc tcgggtcctg cttgaggcac tcgtcgagca tcgcgtcgga gatctggtcg
      541 cagagcttgt ccgggtgacc ctcggtcacg gactcgctgg tgaagagcac gcgctccgtg
      601 gacatgaagg agctcgacat gcctacgtcc cagccttcca ggagttcgcg tacacccgct
      661 gctcctcgct catcgcatcg atgtgcatgc ccattgccgc gagcttgcgc cgggcgatct
      721 cttcgtccag ctcgcggggc acagggtaga cctgtggcgc gagcgtcttc gcgttcttga
      781 ccatgtactc ggccgagagc gcctgattgg cgaacgacat gtccatgacg gccggggggt
      841 gcccctcggc ggccgccagg ttcacgaggc ggccgtcggc cagcacgacg acgctccgcc
      901 catcgcgcag ccggtactcg ttcacgaacg ggcgcagctc ccggcagccc gacgccagcg
      961 tggtgagggt cgcgaggtcg atctcgtcgt tgaagtggcc ggcgttcgcc acgatcgcgc
     1021 cgtccttcag cttggcgagc gcgtccttgc cgatgacgtt cacgttgccg gtcgcggtga
     1081 tgaagaggtc gccacgcgcg gcggcttcga ccatggtcgt cacctcgaac ccgtccatgg
     1141 ccgcctcgag cgcgcggaac ggatcgatct cgcagacgat ggtgatggcg cccatgccgc
     1201 gcaggcggct cgcgatgccg cggcccaccc aaccgtagcc ggcgacgacg gccgtcttcc
     1261 cggcgaggag gaggttcgtc gcgcgaatga ccccgtcgat cgccgactgc cccgtgccgt
     1321 agcggttgtc gaagaaatgc ttcgtgagcg cgtcgttcac ggcgacgatg ggatacgcga
     1381 ggacgccttc ggaggccatc gcgcggagac gcgtgacgcc ggtggtcgtc tcctcggtgc
     1441 cgccggtgac ctcgggcagc agctcgcgcc gcttggtgtg cagctccgtc acgaggtcgg
     1501 cgccgtcgtc catggtcatc tgcggcttcg tcgcgagcac cgcgttgatg tgcgagtagt
     1561 acgtgtcgcg gtcctcgcct ttgcgcgcgt agaccgagat gccgtcgttc gccacgagcg
     1621 aggcggcgac atcgtcctgc gtcgagagcg ggttcgaggc gcagagggcg acgctcgccc
     1681 cgcccgcctt cagggcgagc atgagattcg ccgtctcggc ggtcacgtgg aggcaggcgc
     1741 cgatgcgcag ccccgcgagc ggacgctcct tcttgaagcg ctcggcgatg ccggcgagga
     1801 cgggcatctc gcgcgccgcc cacaggatgc gacgctcccc cgcccccgcg agcccgatgt
     1861 cgctcacgtc gtgttcgcgt gtcttgactg tcgcgctcgt ggtggtcatc gcatcctccc
     1921 aaggatccgg tccaacgccg atcgacgcac cgccggacct tcgatgaacg gtgcgtgcac
     1981 gtggtatccg agccgttcgt agacggcgat cgcgggcgcg ttgtcctgcc gcacgttgag
     2041 cacgacgtcg cgatggtcct cgagcgcggc ctgcgtcacc gccgacgtga ccgacgtcgc
     2101 catgccccgg tcgcggtgac cgagcttcgt gagcacgttg ccgaccgcgg cgagaccggc
     2161 gcgcaccgat cgcacgtgcg tgccggcggc cgacacgagc gcgtcgccga cgtagatgcc
     2221 gaaatagatc tcgcggcgca gacggtcggg cgtgaagtac gtgcgcgagg cgtgaccgta
     2281 aaggtcgatg atgtcctcga ggtgctctgg gccgagccgc gcgacgcgat gcgtcacgcg
     2341 cggattgaag gcctggacgt cgacggccat gcgatgcatg cggtcgacgc gctcgaagcg
     2401 atacgcggcc tccgccgcga ggcgtcccgc cggcggggtc gatatgacca ggcgcgactc
     2461 gcggatgccc tcgcggtaca cggccgcgag cgcctcggtc tcgccgatgg cgaagcacgg
     2521 gcggaaggcc agggcctcga cgaccagcgc ggccgcgacc ggctcgtcac cgcgacgcgc
     2581 gagccaccag cgcgcgcgct cgaaatccgt cgcgtcgaga tccgcgagcg cgtacgcggc
     2641 gagcaggcgg tcccgctcga ggaacgcgcg caggcgttcg ggcggaactt gctcgtcaac
     2701 gcggatctcg ggcaggagcg gcgccgcggt catcggtcct ccttcgatga aaggaccaag
     2761 cgggcctccg ccggtcgcaa ggggtgcgag aagattctac cggctgccga gtgggctcgg
     2821 ccggtaccgg ccgcgctagt agctgatcag cgtgacgacg tcgtacccgc cgagcttgtc
     2881 tcggccgtgc agggcggtca gctcgatgag gaaggcgcag gccacgatct cgccccggag
     2941 ctgcttcacc agctcgatcg ttccggcgac cgtgccgccc gtggcaagca gatcgtcgac
     3001 gatgagcacc ttcgcgcccg gcgcgagcgc gtccctgtgc atctccagcg tcgccgagcc
     3061 gtactcgagg tcgtactcca ccgagacgac gtcccccggg agcttgccga gctttcggac
     3121 cgggacgaag ccgactccga gcttgtacgc gatgggagct ccgaagatga atccgcgcga
     3181 ctccataccg accaccgcgt ccacgtcgcg cttgccgacc ttctcgagga ggccatcgat
     3241 ggatgcgcgg aaggccgctc cgtccttgag gagcgtcgtg atgtccttga agaggatccc
     3301 gggcttcggg aaatccgcga cgtcgcggat gcgtccggag aggtccttca ccgtcgtggc
     3361 cacgccggaa gactacttgg tccggatgcc gcgctgccac gcggccaggg ccggcttcgc
     3421 ggcgccgtcg gtgcggcgga tccccacgct gtcgaagagg ctcgatggga cgccgagctc
     3481 gaacgggaac gaccagagcg cgatcgctgg cctcatcccc gccgtcagcg cgaagaagcg
     3541 agcgacaaac tccgcctgat cgtcgggcga ggcgtcgtac gcggcgaccg cgctcgccgg
     3601 tcgggccgac ggccagccga tctcggtgaa cgcgaccggc gcgcgcagcc gtcgcgccgc
     3661 atcgctgtag tagtcctccg gaaggtcgga tggacgctgg tactcgagga acggatacgt
     3721 cgtgagcccg agaaggtcca gcctgcccgc gaaacgctcg agcagatccc actgcggctc
     3781 gtcgtcgcgt ccggtcatgc gcgtggcgtg acccctcatc agctcgagct ggaagatcgt
     3841 gaacaccttc gtgtccggcg agaccttctt caccgcgtcg tacgcgtcgg cgtagccacg
     3901 gacgaatccg tcgaaggagg tgcggtccga tagccagagc cggttcgtct cgacaccgag
     3961 cgcgaggaag cgcggcctgt ggtcgcgtgc gacctgggtg accgtcgcga ggaagcgctc
     4021 gcgttgcgtc gagctccagt cgatggtgct cttcacgcca gtgccgccgg cgtcggccgc
     4081 gacgccgagc gcgatgacga gcggtccgaa cgagtactgc ttcgccgcgg cgaaggccgc
     4141 gggcacgacg gcgggcacct ttccctcggt gtcggcacgg tcggcccagt tcgtgtacac
     4201 gccgagggcg ccacccgtct cggcgaacga gcgatacatg gcctcggcgt ccgcggcggt
     4261 cgagttcggc gcgtgtggac gcacgagccc ggcgacgccg tagacgatcc cggcggacgc
     4321 ggcggacggg gtggccgtcg gcgcggccgt ttgacacgcg gcgagaacga gcgcgagcgt
     4381 gacgccgcgc gcgaagcggc tcacgagcga gagaccacgt gcgcgctctc gaagagacgc
     4441 tcgacgcgac cggtctcgcc gtcggtgacg atgaccgtga cgttcgtgtt cgtcgcgggt
     4501 gaccggtcga gcttcggcag gaggccgagg tagtgcgaca cgaggatgcg ggtgacgcct
     4561 ccgtgtccga tcacgagcac cgtcccgccc gatgtctcgc gcgccagccg gcggaccgcg
     4621 cgcaggcccc gctccaggac ctgctcgagc gactcgccgc cgggccagga cacgaagccg
     4681 tcgtccttcc actccggcag gccgcgcacc tcgtcgaagt gcgcgccggt gcggtcgccg
     4741 acgtcgatct cgacgagacc cggctcgacg tccacgggca tccgccggcg gcgcgcgatc
     4801 acgagcgccg tgtccagcga gcgcgacagc ggcgtggcca cgatgcgatc gaacggcacc
     4861 tcggcgagcg cgtccgcgac ggcgacggcc tgctggcgcc cgcgctccgt gagcgggctg
     4921 tcggagcggc ccgagaagcg gtgggacgcg ttcgcctcgc tctcgccgtg gcggacgaag
     4981 aagaaggtgg tcactagacg ccgaggcgcg cggcgacgtc ggaccagccg ttcacgcgga
     5041 tgatccgctc gtgcgacacg tcgcgattcc acggatggtc gaacagcagg acgcggacgc
     5101 cggcctcggc gagcgcctcc gagatgatca cgtcgtcctc gcagaacagg tcgagccgca
     5161 gctcctgcgc gagccgcacc ttgtacgcgg cggagctctg cgggtcgtac cgcccgcgcg
     5221 ggtagtcggg gttgaactcg ccgtgcggct tgaggtggac ggccttcgcg tagtcgagga
     5281 tgcgtttctc gcgcagccac gtctcggtca ccatgcggcg cgcctcgttc cgcgcggtga
     5341 tgaagtagat ctcgtgtccg cgctggacga gctgctcgag gacgatcggg cagtcctcgt
     5401 agatgtcgag gtcgctgaag aaggtgccgt cgtacagacg catgcggaac cggtcgcgtt
     5461 cgcgcgccgg gaccttgtcg tccatctggc ggagcagatg ccagccgatc tcctgctcgt
     5521 ccgggagctc gaccttgtac tccgcggcga acttccgcag gtacgggacc gcgcacaccg
     5581 cgatgacgtc gtcgaggtcc accccgatct tcattcctcg cgctcgctgt cggactcgct
     5641 cctcgcggga ggcccgctcg tcgctcgact cgatctcata cccacaaccc tacgaggagc
     5701 gtggcgtcga acgcccagtg cgcgacgacg ggcgcggcga tgccgccgga gcggatgcgg
     5761 agcagcgcga ggacggcgcc accgatcgcg acgaacacga gcgcggccat cccggcgagc
     5821 catgcgagcg ccgtgcccgc aaggctcgtc tgcagcaggg tcacccacac cacgaacgcg
     5881 tgccacagcg cgaacgccgc cgacgatccc acgaccgcct tccgtgcgcc tgtcctcgtc
     5941 cacaggccgc cgagcaggac gccgcggaag gcgacctcct ccgggatcac cgcggcgagc
     6001 ggcaggaaga ggacgatgtg cagcgcgagc gcgtcgggcg tggtcgcgga gagcggctgg
     6061 tagccgatcg gcgcgccgac gatcggcccc acgcgcagca cggcggtgcc gacggcggcg
     6121 gccacgagcg cgaccgccgc ggcggcgccg atgccgcgcg gatcgccgcg caccagaccg
     6181 aggtcgcccc gccgctgccg cagcaggcgt gcgacgccga gcgagaccgc aacgagggcc
     6241 gcgccccagc tcaccgccgc ccagtcaccg ccgggtagcg ccggctggac gaagtaattg
     6301 acgacgttcc cccagatgac gagcagcgcg gcgaagatcc agatcacccg cggatccggc
     6361 tccgccgcgt catgcgacga gatccaggag gcgcgtgagc gcgtcgcgcc agtcgccgcg
     6421 cgtgtccagc accgagaggc gtatctgccg cgtgccggcc tgcacgcgcg ggaacgcctt
     6481 cgcgaggcgg ccgcgacggt cgggtgtgag gtcgctcgcg agcacgagcc gcagctcggg
     6541 gccgcgcgcg cgcacctcgg tcgcgccggc ggcgaccgcg cgcagcttca cctcgacgcc
     6601 gtagaggagg ttgcgcacgg gctcgggcgg cgggccgtag cggtcgcgca gctcgtcgcg
     6661 cagcgccgag agctcctcgg tcgagcggag ctcgccgagc cggcggtaca gctcgagctt
     6721 ccgttgccgg ctcgggacgt agtcgtccgg gatcgcggtg gagagcgcga ggtcgaccgt
     6781 gacctcgggc agcgcctccc gctgcggcgc gccgcgctcg gcgttcaccg cctcctcgag
     6841 catctgcgtg tacagctcga agccgaccgc cgccacatgg ccgtgctgct cggcgccgag
     6901 gatgttcccg gcgccgcgga tctcgaggtc gtgcatcgcg atcttgaagc cggcgccgag
     6961 ctcgctcgcc tcgaacaccg cctgcagccg cttgcgcgcg tcctccgaga ggcgctcctg
     7021 cttcgaatag agcaggtatg cgtacgcctt cgccgcggcg cggcccacgc ggccgcggag
     7081 ctgatagagc tgcgcgaggc cgagttgtcc ggcgtgatcg atgacgatcg tgttcacgtt
     7141 cgggatgtcg agccctgact cgatgatcgt cgtacacacc agcacgtcgt gctcggcgtc
     7201 ggcgaactcc accatgacgc gctcgagctt tcgttcgtcc atctggccgt gcgcgacgac
     7261 gaaccgcgcg ttcggtacga gcacgcggag ccgccgcgtc tcgtgctcga tgccctgcac
     7321 gcggttgtgg acgtagtaca cctgcccgcc gcggtcgagc tcgcggagga tcgcctcgcg
     7381 caggacatcg tcggtccgtt cgacgacgtg cgtctcgatg ggctgacggt cctcaggcgg
     7441 cgtctcgatg acgctgatgt cgcggatccc cgagagcgcc atgtgcagcg tccgcgggat
     7501 gggcgtcgcg gtcatcgaga ggacgtgcac ctcggtgcgc atctgcttca ggcgctcctt
     7561 gtgcttcacg ccgaagcggt gctcctcgtc gatcacgagc agcccgaggt cacggaagcg
     7621 cacgtccttc tgcaggaggc ggtgcgtccc gatgacgacg tc
//
LOCUS       DASTZR010000509         2472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_417589, whole genome shotgun sequence.
ACCESSION   DASTZR010000509 DASTZR010000000
VERSION     DASTZR010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417589"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2042
                     /locus_tag="VFV20_11275"
     CDS             <1..2042
                     /locus_tag="VFV20_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HEU5288974.1"
                     /translation="PGMYIGTTDLRGLHHLVREVLDNSIDEAMNGTCDRIDVTIHKSN
                     EITVADNGRGIPTDKQKQTGKSALEVVHTILHAGGKFGGAGYKVSGGLHGVGVSVVNA
                     LSTELIVEVHRDNKVWTQTYSRGKPEGAVKTIAKPTLPLPPVLAWKERAKSTGTVTRF
                     WPDPQMFPVIEWDAQIVEQWLRETAYLNKGLFLSLHDERNGNEANYYFDGGIVSFVRH
                     LNRSHAVLHKPVYAERSHDNGTVVEVSLQYNDGFAEKVYTFANNINTIDGGAHLTGFR
                     NALTRTINAYARKNGQLKESDPNLTSDDVREGLTAVVSVKIREPQFEGQTKTRLGNAE
                     VAGQVQAAVNDALGQYLEENPPDARRIVEKCLTAFRAREAARKARDLVLRKGALDGFS
                     LPGKLADCSERDPERCELIIVEGQSAGGSAKSGRDRRFQAILPLRGKILNVEKARADK
                     MLGNEEIKSLITALGTGIGDYFDMKKLRYGKTILLADADVDGSHIRTLLLTLLYRHFK
                     PLVEEGRIYIGQPPLYRLQLGKDVRWAYSDQERDKTIKELKSTAKKKRDDRRAKAADA
                     EEKTAKGRRRAAAATAAEDAAEAEANANASTDEGTDDGKGGREPAIARYKGLGEMNAE
                     QLWETTLNPATRTLKKVTLDDAEEADHVFDELMGNEVEGRKKWIMANAKHAELDI"
     gene            2042..>2472
                     /locus_tag="VFV20_11280"
     CDS             2042..>2472
                     /locus_tag="VFV20_11280"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU5288975.1"
                     /translation="MARLSELGIDFDHWCFACGRANPIGLHLEYDVARSRAETRFTPR
                     REHTGYDGLVHGGIVTALLDETMGWAIFYQGIWAVTARITVTFKQTVPVGEPLVVSGE
                     VARDRGRAIETRGLLRRERDDEVLAEADALFLRMPEEKRAL"
BASE COUNT          476 a          844 c          831 g          321 t
ORIGIN      
        1 ggccgggcat gtacatcggc acgacggacc ttcgcggcct gcatcacctc gtccgcgagg
       61 tcctcgacaa ctcgatcgac gaggcgatga acggcacgtg cgaccgcatc gacgtgacga
      121 tccataagag caacgagatc acggtcgccg ataacggccg cggcatcccg accgacaagc
      181 agaagcagac cggcaagagc gcgctcgagg tcgtccacac catcctccac gccggcggca
      241 agttcggcgg cgccggttac aaggtctccg gcggcctcca cggcgtcggc gtgtcggtcg
      301 tgaatgcgct ctcgaccgag ctcatcgtcg aggtgcaccg cgacaacaag gtctggacgc
      361 agacgtactc gcgcggcaag cccgagggcg cggtgaagac gatcgcgaag ccgacgctcc
      421 cgctcccacc ggtcctcgcg tggaaggagc gcgccaagtc gacgggcacg gtgacgcgct
      481 tctggcccga tccgcagatg ttcccggtca tcgaatggga cgcgcagatc gtcgagcaat
      541 ggctccgcga gaccgcgtac ctgaacaagg gcctcttcct ctcgctgcac gacgagcgca
      601 acggcaacga ggcgaactac tacttcgacg gcggcatcgt gtcgttcgtg cgccacctga
      661 atcggtcgca tgcggtgctg cacaagcccg tttacgcgga gcgctcgcac gacaacggga
      721 ccgtcgtcga ggtctcgctg caatacaacg acggcttcgc cgagaaggtc tacacgttcg
      781 cgaacaacat caacaccatc gacggcggcg cgcatctcac gggcttccgc aacgcgctca
      841 cgcgcacgat caacgcgtac gcgcgcaaga acggccagct gaaggagtcg gaccccaacc
      901 tcacctccga cgacgttcgc gagggcctca ccgccgtcgt gagcgtgaag atccgcgagc
      961 cgcagttcga gggtcagacg aagacgcgcc tcggcaacgc cgaggtcgcg gggcaggtcc
     1021 aggccgcggt gaacgacgcg ctcggccagt acctcgagga gaacccgccg gacgcgcgcc
     1081 gcatcgtcga gaagtgcctc accgcgttcc gcgcacgtga ggcggcacgc aaggcgcgcg
     1141 acctcgtgct ccgcaagggc gcgctcgacg gcttctcgct gcccggaaag ctcgccgact
     1201 gctcggagcg cgatccggag cgatgcgagc tgatcatcgt cgagggccag tccgccggcg
     1261 gctcggccaa gtccggccgc gaccgccgct tccaggcgat cctgccgctg cgcggcaaga
     1321 tcctgaacgt cgagaaggcg cgcgccgaca agatgctcgg caacgaggag atcaagtcgc
     1381 tcatcaccgc gctcgggacg ggcatcggcg actacttcga catgaagaag ctgcgctacg
     1441 gcaagaccat cctgctcgcc gacgcggacg tcgacggctc gcacatccgc acgctcctgc
     1501 tgacgctgct ctaccgccac ttcaagcccc tcgtcgaaga gggccggatc tatatcggcc
     1561 agccgccgct gtaccggctg cagcttggga aggacgtgcg ctgggcctac tccgaccagg
     1621 agcgcgacaa gaccatcaag gagctgaagt ccacggcgaa gaagaagcgt gacgatcgga
     1681 gggccaaggc ggcggacgcc gaggagaaga ccgcgaaggg ccgccgccgt gccgcggccg
     1741 cgaccgcggc cgaggacgcc gccgaggcgg aggcgaacgc gaacgcgtcg accgatgagg
     1801 gcaccgacga cgggaagggc ggccgcgagc cggcgatcgc gcgatacaag ggtctcggcg
     1861 agatgaacgc cgagcagctg tgggagacca cgctcaaccc cgccacccgc acgctgaaga
     1921 aggtcaccct cgacgacgcc gaggaggcgg accacgtctt cgacgagctc atgggcaacg
     1981 aggtcgaggg ccgcaagaag tggatcatgg ccaacgcgaa gcatgcggag ctcgacatct
     2041 gatggcacgg ctcagcgagc tcggcatcga cttcgaccac tggtgcttcg cgtgcggccg
     2101 tgcgaatccg atcggtctgc acctggagta cgacgtcgcg cggtcgcgcg ccgagacgcg
     2161 cttcacaccg cgccgcgagc acaccggata cgacggcctc gtgcacggtg ggatcgtcac
     2221 cgcgctgctc gacgagacga tgggctgggc gatcttctac cagggaatct gggcggtcac
     2281 cgcgcgcatc acggtgacct tcaagcagac cgtccccgtg ggggagcccc tcgtcgtctc
     2341 cggagaggtc gcgcgcgacc gcggccgcgc gatcgagacg cgtggattgc tgcggcgcga
     2401 gcgtgacgac gaggtcctcg cggaggcgga cgccctgttc cttcgcatgc cggaagagaa
     2461 gcgcgctctc at
//
LOCUS       DASTZR010000510         2335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_390788, whole genome shotgun sequence.
ACCESSION   DASTZR010000510 DASTZR010000000
VERSION     DASTZR010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390788"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..1620)
                     /gene="gltA"
                     /locus_tag="VFV20_11285"
     CDS             complement(121..1620)
                     /gene="gltA"
                     /locus_tag="VFV20_11285"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008382865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HEU5288976.1"
                     /translation="MAPKKRTIRTIPADRTPMAEQPPAERARNFGEVACGYTLADALR
                     ESDRCLLCPDEPCVRGCPVGIDIPGFIAKIGEKDIRGAYDVLSDTTLLPAICGRVCPQ
                     ESQCEAVCTVKQTYEPVAIGRLERFVGDMAISESWSAVPYIEKAPFKVGIVGSGPAGM
                     ACAADMAKAGCQVTVFEAFQQPGGVLRYGIPDFRLPNEVVDAEIAKLTKLGVTFRTNV
                     LVGRLFTIEQMVGEMGFDAVFIGVGAGTPAFMDIPGESLNRVVSANELLTRVNLMHAR
                     DFPSYDTPLGIGKKVAVIGSGNTAMDAMRVSLRLGAETVMCVYRRSRVEAPARAEELH
                     HAEQEGIQFHWLTNPIEILDDGQRGVGGLRCQRMELGEPDASGRRRPVAIPGSEFVLE
                     CDTVVCAIGTNANPILGQTSHLKLDARGYIEVDDDLATSLDGVFAGGDIVTGAATVIE
                     AMGAGRKAAASMKRYLGLVDSDVPYRDESAPTLFGLAPEQREFARVRVA"
     gene            complement(1621..>2335)
                     /locus_tag="VFV20_11290"
     CDS             complement(1621..>2335)
                     /locus_tag="VFV20_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008382863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HEU5288977.1"
                     /translation="ITLVVQAVGKTTKEMQRDLKAGSHLYAVVGPMGEPSHLGAKKVV
                     CVGGGLGVAPVYPQARAFKESGAYVIGVIGFRNKDLVFWEDKFRAVCDEFIICSDDGS
                     VGIKGLITEGIKLAASQHNDIDQLIAIGPPIMMRVASELTKALGIKTVVSLNPVMVDG
                     TGMCGGCRVKVGGRTRFACVDGPEFDAHTVDFDDLMFRLARFKPDEKASLERWQQGCR
                     VETQAVPAAAVASAAPKAS"
BASE COUNT          337 a          819 c          807 g          372 t
ORIGIN      
        1 cgccgaagga ctgaccgcag cgctgcgcgc cctcgccgga gtcgctcacg atctcgacga
       61 cgtgctcctc gagcttcgtg acccgcttcg ccgtggcggc cttctggatc gtctcggtgg
      121 tcatgccaca cgcacccgcg cgaactcgcg ctgctcgggt gcgagcccga agagcgtggg
      181 cgcgctctcg tcgcgatatg gcacgtcgct gtcgacgagg ccgaggtagc gcttcatcga
      241 agcggcggcc ttgcggccgg cgcccatggc ttcgatgacg gtcgccgcgc ccgtcacgat
      301 gtcgcccccc gcgaacaccc cgtcgagcga tgtcgcgaga tcgtcgtcga cctcgatgta
      361 gccgcgggcg tcgagcttca ggtgcgaggt ctggccgagg atcgggttcg cgttcgtgcc
      421 gatcgcgcag accaccgtgt cgcattcgag cacgaactcg ctccccggga tcgcgaccgg
      481 gcggcgccgg ccggaggcgt cgggttcgcc cagctccatc cgctggcagc gcaggccgcc
      541 gacgccgcgc tgaccgtcgt cgaggatctc gatcgggttg gtgagccagt ggaactggat
      601 gccctcctgc tcggcgtgat gcagctcctc ggcgcgcgca ggcgcttcga cccgcgaccg
      661 gcggtacacg cacatgacgg tctccgcgcc cagccgcagg gacacgcgca tcgcgtccat
      721 cgcggtgttg ccggagccga tcaccgcgac cttcttcccg atcccgaggg gcgtgtcgta
      781 cgaggggaag tcgcgcgcgt gcatgaggtt cacgcgtgtg aggagctcgt tcgcggacac
      841 gacgcggttc agcgactcgc cggggatgtc catgaacgcg ggcgtgccgg cgccgacgcc
      901 gatgaacacc gcgtcgaagc ccatctcgcc caccatctgc tcgatggtga acagacgccc
      961 cacgagcacg ttggtgcgga acgtgacgcc gagcttggtg agcttggcga tctccgcatc
     1021 cacgacctcg ttcggaagcc ggaagtccgg gatgccgtag cggagcacgc cgccgggctg
     1081 ctggaaggcc tcgaagaccg tcacctgaca gcccgccttc gccatgtcgg ccgcgcaggc
     1141 catgccggcc ggaccggaac cgacgatgcc caccttgaac ggggccttct cgatgtacgg
     1201 cacggcggac cagctctcgc tgatggccat gtcgccgacg aagcgctcca ggcggccgat
     1261 ggcgacaggc tcgtacgtct gcttcaccgt gcacaccgcc tcgcactgcg actcctgggg
     1321 gcagacgcga ccgcagatcg cgggcaggag cgtggtgtcg gagagcacgt cgtaggcgcc
     1381 gcggatgtcc ttctcgccga tcttcgcgat gaacccgggg atgtcgatgc cgaccgggca
     1441 tccgcgcacg cagggctcgt ccggacagag caggcagcgg tcggattcgc gcagcgcgtc
     1501 cgcgagcgtg tacccgcacg cgacctcgcc gaagttgcgc gcccgctcgg cgggcggctg
     1561 ctcggccatg ggggtgcggt ccgccgggat cgtgcggatg gtccgcttct tcggtgccac
     1621 ttacgaggcc ttcggggccg cgctcgccac ggcggcggcg ggcacggcct gtgtctcgac
     1681 acggcagccc tgctgccagc gctcgagtga cgccttctcg tcgggcttga accgcgcgag
     1741 gcggaacatc aggtcgtcga agtcgacggt gtgcgcgtcg aactcggggc cgtccacgca
     1801 cgcgaaccgc gtccggccgc cgaccttcac gcggcacccg ccgcacatcc cggtgccgtc
     1861 caccatgacc gggttgaggc tcacgaccgt cttgatgccg agcgccttcg tgagctcgga
     1921 cgcgacgcgc atcatgatcg gcgggccgat cgcgatgagc tggtcgatgt cgttgtgctg
     1981 cgacgcggcg agcttgatgc cctcggtgat gagccccttg atgccgaccg agccgtcgtc
     2041 gctgcagatg atgaattcgt cgcagacggc gcggaacttg tcctcccaga acacgaggtc
     2101 cttgttgcgg aatccgatca cgccgatgac gtaggcgccc gactccttga acgcgcgcgc
     2161 ctggggatac accggcgcca cgccgagccc gccgccgacg cacacgacct tcttcgcgcc
     2221 gaggtggctg ggctccccca tggggccgac gacggcgtag aggtgcgagc ccgccttgag
     2281 gtcgcgctgc atctccttgg tcgtcttgcc gacggcctgg acaacgagcg tgatc
//
LOCUS       DASTZR010000511         2963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_991968, whole genome shotgun sequence.
ACCESSION   DASTZR010000511 DASTZR010000000
VERSION     DASTZR010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991968"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VFV20_11295"
     CDS             <1..271
                     /locus_tag="VFV20_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288978.1"
                     /translation="REQGVHVSRGDPSAEQPGASIVIPDHPSRLRPRDLDLDDLRRSY
                     VRQLAERGARRLTADETAYVAAETRWTPDRIAREWDAIAATSGTR"
     gene            235..1563
                     /locus_tag="VFV20_11300"
     CDS             235..1563
                     /locus_tag="VFV20_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5288979.1"
                     /translation="MGCDRGDVRHEVKAASGESVIARHDSVISSATRLAFYPLVVTGG
                     EGAMVRGADGRERIDFLASAASLNVGIQHPRVVHAVREQAARLLHYNAAYVYHEPMVE
                     LAERLIAVTPGRGAKRVSFGLSGGDAIDGTIKASRHFTGRTKVIAFDGAYHGTTYGAI
                     SVSSVSREMRAGLGPFLPEVYHATFDDLSSFDALVATRVPAEEVAVVIVEPIQGDSGV
                     QIPTAEFMRGLAERCRTHGMLLAVDEVQTGVGRTGRWFASEHFGLEPDIVVCGKGLAS
                     GMPLSAIVARDEILSSWRPPAHTFSSGANPVTCAAALATLDIVRDEGLVEVSRRRGEL
                     LIARLRELASRHALVGAIRGRGLMVGAELVTDPAVGTPAYRETAKVCWRAWQLGLLIT
                     FLRGNVLRMVPPLVISEAQLDRAVEILDEALTDVERGTVSDEEIARVRGW"
     gene            1803..2327
                     /locus_tag="VFV20_11305"
     CDS             1803..2327
                     /locus_tag="VFV20_11305"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5288980.1"
                     /translation="MTVALDLVREKSSQARARILAVVSDLTDDQLMWRPTQHAHSIGF
                     TLWHTARANDNVQADLTGHPLEWDAGRYAARWGHPDRGTGTGWDDERSAALPLPARDE
                     LFAYVHRVFDAVDAATGALDDAALSRTLRSRFMGAETSIAEIVGSAIGHDNRHLGEME
                     YIKGLLGLRGTATV"
     gene            complement(2331..>2963)
                     /locus_tag="VFV20_11310"
     CDS             complement(2331..>2963)
                     /locus_tag="VFV20_11310"
                     /inference="COORDINATES: protein motif:HMM:NF039288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEU5288981.1"
                     /translation="NAPRPPADEAGGSAWVRLAERLGSLAAQLLDERPGGIELHYAGA
                     LLEIDPEPLRAAVVKGLLETFSEQRVNLVNALAIARARGLDISERRETEAARYRALLT
                     VRVGSTEVAGTLVQGEPRVVFIDGFWVDVPVEGHLLLTKHQDKPGLVGRVGTLLGEHD
                     VNISSMQVGRLHPRGEALMILTLDDPVPDDVRASVGAFPDVDRVRTAKLG"
BASE COUNT          414 a         1074 c         1033 g          442 t
ORIGIN      
        1 ccgcgagcag ggtgtccatg tctcgcgtgg cgacccatcc gcggagcaac cgggggcgtc
       61 gatcgtcatt cccgatcacc cctcgcgtct ccgtccgcgc gatctcgacc tcgacgacct
      121 gcggcgttcc tacgtccgcc agctcgccga gcggggcgcg cgccgcctca ccgccgacga
      181 gaccgcctac gtcgccgccg agacgcggtg gacgcccgat cggatcgcgc gcgagtggga
      241 tgcgatcgcg gcgacgtccg gcacgaggtg aaggccgcat cgggcgagtc cgtcatcgcg
      301 cggcacgata gcgtgatctc gagcgcgact cgcctcgcgt tctatccgct cgtcgtgacc
      361 ggcggcgagg gtgcgatggt gcggggcgcc gacggtcggg agcgcatcga cttccttgcg
      421 agtgcggcat cgctcaacgt gggcatccag cacccacgcg tcgtgcatgc ggtgcgcgag
      481 caggccgcgc gtctgctgca ctacaacgcc gcgtacgtct accacgagcc tatggtcgaa
      541 ctggccgaac ggctcatcgc ggtgacgccc ggccgcggcg cgaagcgcgt gtccttcggg
      601 ctctcgggtg gcgacgccat cgacggcacg atcaaggcat cccgccactt caccgggcgt
      661 acgaaggtca tcgccttcga cggcgcgtat cacggcacga cgtatggcgc gatctccgtc
      721 tccagcgtca gccgcgagat gcgcgccggc ctcggcccgt tccttccaga ggtctaccac
      781 gcgacgttcg acgacctctc ctcatttgac gcacttgtcg cgacccgcgt cccggccgag
      841 gaggtcgcgg tcgtcatcgt cgagcccatc cagggcgaca gcggcgtgca gatcccgacc
      901 gccgagttca tgcgcggcct cgcggaacga tgccgcacgc acggcatgct tctcgcggta
      961 gatgaagtgc agaccggcgt cgggcgcaca ggccgatggt tcgcctcgga gcacttcggt
     1021 ctcgagcccg acatcgtcgt ctgcgggaag ggcctcgcct cgggtatgcc gctgtccgcg
     1081 atcgtggccc gcgacgagat cctgtcgtcg tggcggccgc cggcccacac gttctcgtcg
     1141 ggtgcaaacc cagtcacgtg cgccgcggcg ctcgcgacgc tcgacatcgt gcgcgacgag
     1201 ggtctcgtcg aggtatcgcg ccgccgcggt gagcttctca tcgcgcgtct ccgcgagctg
     1261 gcgtcacgtc acgccctcgt cggcgcgatc cgcgggcgcg ggctgatggt cggcgcggag
     1321 ctcgtgaccg acccggcggt cgggacgccg gcttaccgcg agacagcgaa ggtgtgctgg
     1381 cgcgcgtggc aactcgggct gctcatcacc ttcctgcgtg ggaacgtgct gcgtatggtg
     1441 cccccactcg tcatcagcga ggctcagctc gatcgcgccg tcgagatcct tgatgaggcg
     1501 ctcaccgacg tggagcgcgg cacggtcagc gatgaggaga tcgcacgggt ccgcggctgg
     1561 taggcgcgcc tcgggcgtga cccgccgacc ttgccgccga gcttcgcggt atcacgcggt
     1621 cgacgtgggg aacgcgcccg cgcaacctcg cgctcgcgtc gctcggagtg gcggcgacgt
     1681 acgggctcgc cgcgatcacc gtcctcgccg tgcaggccat cgccctcgcg gtgctgcgtc
     1741 gtgcgccgcc cgagatggag gcggtgggct gacgtggtga tcccgggtta cgctcgcccg
     1801 ccatgaccgt cgccctggat ctggtgcgcg agaagagcag ccaggcccgc gcgcgcatcc
     1861 tcgccgtcgt gagcgacctc accgacgacc agctgatgtg gcgacccacc cagcacgcgc
     1921 acagcatcgg gttcacgctc tggcacaccg cgcgggccaa cgacaacgtg caggccgatc
     1981 tcaccggcca cccgctcgag tgggacgccg gtcgctacgc ggcgcgctgg ggtcatccgg
     2041 accgcggcac cggcacgggc tgggacgacg aacgttcggc ggcgctcccg ctacccgcgc
     2101 gcgacgagct cttcgcgtac gtgcaccgcg tcttcgacgc cgtcgacgcg gcgaccggag
     2161 ccctcgacga cgccgcgctc tcgcgaacgc tgcgcagccg tttcatgggc gcggagacct
     2221 ccatcgctga gatcgtgggc agcgcgatcg gccacgacaa ccgccacctg ggcgagatgg
     2281 agtacatcaa gggtctcctg gggctgcgcg ggacggcgac cgtctagggg tcagccgagc
     2341 ttcgcggtac gcacgcggtc gacgtcgggg aacgcgccca cgctggcacg gacgtcatcg
     2401 ggcaccggat cgtcgagcgt gaggatcatg agggcctcgc cgcgcggatg cagccgaccg
     2461 acctgcatcg acgagatgtt cacgtcgtgc tctccgagga gcgtgccgac gcggcccacc
     2521 agaccgggct tgtcctggtg cttcgtcagg aggagatggc cctccaccgg cacgtccacc
     2581 cagaagccgt cgatgaacac cacgcgcggc tcaccctgga cgagcgtgcc ggcgacctcg
     2641 gtgctgccga cgcgcaccgt gagcagggcg cggtaacggg ccgcctccgt ctcgcgtcgc
     2701 tcggagatat cgagcccgcg cgcgcgcgcg atggcgagcg cgttcaccag gttcacccgc
     2761 tgctcgctga aggtctcgag cagccccttc acgaccgcgg ctcgcagcgg ctccgggtcg
     2821 atctcgagga gcgcgccggc gtaatgcagc tcgatgccgc ccggccgctc gtcgagcagc
     2881 tgtgccgcga gactgccgag acgttccgcg agcctcaccc acgcgctgcc gccggcctcg
     2941 tccgcgggcg ggcgcggcgc gtt
//
LOCUS       DASTZR010000512         2947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_51647, whole genome shotgun sequence.
ACCESSION   DASTZR010000512 DASTZR010000000
VERSION     DASTZR010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51647"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /locus_tag="VFV20_11315"
     CDS             <1..1244
                     /locus_tag="VFV20_11315"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HEU5288982.1"
                     /translation="AESALRQAKDVITKEFGAQYALERPRHYKTRSKGAQEAHEAIRP
                     TDLSRTPTYAKKYLKPDQLKLYTLIWQRTLASQMAPAKFENTRLDVEAGHYVLRANGR
                     RVTFDGFIRVYTEGSDDPEKEISPLPAVVAGEELKLLGLESTQHFTQPPPRFTEASLV
                     KTLEEFGIGRPSTYAPTISTLVGRRYVRKEGRALIPEDVGFVVTDFLKEHFADIVDTG
                     FTARMELDLDKIAEGEVEWAPVVRDFYGPFAKRVEEKTKSVKKSDVTEEATDKLCPKC
                     GRPVVIKLGRFGRFYSCTGFKKTKKGDPQPPGACDYAEPLEGQQEPQLEIIEGEMCPE
                     CGKPLARRRGRFGPFIGCTGYPDCKYVKKTLQKTGVVCPDCGKGELVRRRGRGRSTFF
                     GCERYPECTYTARELPTKDAA"
     gene            1245..2225
                     /locus_tag="VFV20_11320"
     CDS             1245..2225
                     /locus_tag="VFV20_11320"
                     /inference="COORDINATES: protein motif:HMM:NF014445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEU5288983.1"
                     /translation="MLARPADAAEEGRWDELVVATGRPHILQSRAWADLKALTGWGVR
                     RFVFDGGVAQVLTKRLPLGITVAYAPRGPLVEPAGLTAAILALRDALAKERCASLLCD
                     PELAPDEALRAELERSGVRRSPVFVQPRRTLLMDLTLDPGAMQAAMRKKTRQYINKGE
                     REGVVTEETRDLDRFLRVLRAVGERDKFALHSREYFERLLGAFGDRAHMMIARVGDED
                     AGALMLARMSDRAWELYGGWSGAHAESRPFYLLKWRAMMRMRALGARRYDMWGLAEGA
                     DDPLAGVENFKLGFGGQVTEWIGALETPVRPMLYPVWQLAARRRLARSAA"
     gene            2231..>2947
                     /locus_tag="VFV20_11325"
     CDS             2231..>2947
                     /locus_tag="VFV20_11325"
                     /inference="COORDINATES: protein motif:HMM:NF014445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEU5288984.1"
                     /translation="MAAPEGWDDAAVRSPWGHTLQSSAWAEVRAAQGWRPEFVRIGDP
                     LPVALVLWRDALPGRPIAYVPRGPIVAPDDRAGLVAALRRLAALARERRALFLKVDPE
                     LDPSIAGPALAEAGYRRSPQDIQPVLATLELDLGADEDALMAGLEKDTRWSVRQAAKK
                     GVTVREVADDAGLRAFYELYAATGERAGFITRTWTYYRETWGRLVAAGLATVRLADVE
                     GEAVAGAMVWRCGDRALYQTA"
BASE COUNT          445 a         1005 c         1088 g          409 t
ORIGIN      
        1 tcgccgagag cgcgctgcgc caggcgaagg acgtgatcac caaggagttc ggcgcgcagt
       61 acgcgctgga gaggccgcgg cattacaaga cgcgctcgaa gggcgcgcag gaggcgcacg
      121 aagccatccg gccgacggac ctctcgcgca cgccgaccta tgcgaagaag tacctcaagc
      181 ccgatcagct gaagctctac acgctgatct ggcagcggac gctcgcgtcg cagatggcgc
      241 ccgcgaagtt cgagaacacg cgcctcgacg tcgaggccgg gcactacgtg ctccgtgcga
      301 acgggcgccg ggtcaccttc gacgggttca tccgcgtgta caccgagggc agcgacgatc
      361 ccgagaagga gatctcgccg ctgccggcgg tcgtcgcggg cgaggagctg aagctgctgg
      421 gcctcgagag cacgcagcac ttcacgcagc cgccgccgcg gttcaccgaa gcgtcgctgg
      481 tgaagacgct ggaggagttc ggcatcggcc gtccgtccac ctacgcgccc acgatctcga
      541 cgctggtcgg ccgccggtac gtccgcaagg agggccgtgc gctgatcccc gaggacgtcg
      601 gcttcgtcgt gaccgacttc ctgaaggagc acttcgcgga catcgtggac accggcttca
      661 cggcacgcat ggagctcgac ctcgacaaga tcgccgaggg tgaggtggag tgggcgccgg
      721 tcgtgcgcga cttctacggg ccgttcgcca agcgcgtcga ggagaagacg aagagcgtca
      781 agaagtcgga cgtcaccgag gaggccaccg acaagctgtg tccgaagtgc ggccgcccag
      841 tggtcatcaa gctcggccgt ttcggccgct tctactcgtg caccggcttc aagaagacga
      901 agaagggcga cccccagcct ccaggcgcat gcgattacgc cgagccgctc gagggtcaac
      961 aagagccgca gctcgagatc atcgagggtg agatgtgccc cgagtgcggg aagccgctgg
     1021 cgcggcgccg cgggcggttc gggccgttca tcggctgcac cggctacccg gactgcaagt
     1081 acgtgaagaa gaccctccag aagaccggcg tcgtctgccc cgactgcggg aagggcgagc
     1141 tcgtccggcg ccgcggccgc ggacgctcga cgttcttcgg ctgcgagcgg tatccggaat
     1201 gcacgtatac cgcgcgtgag ctccccacca aagacgccgc ctaggtgtta gcgcgacccg
     1261 cggacgccgc ggaagagggg cgctgggacg agctcgtcgt cgccaccggg cgcccacaca
     1321 tcctccagtc gcgtgcgtgg gcggacctga aggccctcac cggctggggc gtgcgccgtt
     1381 tcgtcttcga cggtggggtc gcgcaggtcc tgacgaagcg cctgccgctc gggatcaccg
     1441 tggcgtacgc accgcgcggt ccgctcgtcg agcctgccgg cctcaccgcc gcgatcctcg
     1501 cgctgcgcga cgcgctggcg aaggagcgct gcgcgagcct gctctgcgat ccggagctcg
     1561 cgccggacga agcgctccgt gccgagctcg aacgcagcgg cgtgcggcgg tcgccggtgt
     1621 tcgtgcagcc gcgacgtact ctgctcatgg acctcacgct ggatccgggc gccatgcagg
     1681 ccgcgatgcg caagaagacg cgccagtaca tcaacaaggg cgagcgcgag ggcgtcgtca
     1741 ccgaggagac gcgcgacctc gaccgcttcc tccgtgtgct gcgcgcggtc ggcgagcgcg
     1801 acaagttcgc gctgcactcg cgggagtatt tcgagcggct gctcggggcg ttcggcgacc
     1861 gggcgcacat gatgatcgcg cgcgtgggtg acgaggacgc gggcgcgctg atgctcgcgc
     1921 gcatgtccga ccgcgcgtgg gagctctacg gcggatggag cggcgcgcac gccgagtcgc
     1981 gtccgttcta cctcctcaag tggcgcgcga tgatgcgcat gcgcgcgctc ggtgcccggc
     2041 gctacgacat gtggggtctc gcggagggcg cggacgaccc gctcgccggg gtcgagaact
     2101 tcaagctcgg attcgggggc caggtgacgg aatggatcgg cgcgctcgag acgccggtgc
     2161 ggccgatgct ctacccggtg tggcagctcg ccgcgcggcg acgcctcgcg cggtccgcgg
     2221 cctgaacgcc gtggccgcgc ccgaaggctg ggacgacgcg gccgtccgct ctccgtgggg
     2281 tcacacgctc cagtcgagcg cgtgggctga ggtgcgcgcg gcgcaggggt ggcggccgga
     2341 gttcgtgcgc atcggggatc cgctcccggt ggcgctcgtg ctctggcgcg acgcgctgcc
     2401 cggacgtccc atcgcgtacg tcccgcgcgg tccgatcgtc gcgcccgacg atcgcgcggg
     2461 gctcgtcgcg gcgctgcgcc ggctcgccgc gctggcgcgc gagcgtcgcg cactgttcct
     2521 caaggtcgac cccgagctcg acccgtcgat cgcgggcccg gcgctcgcgg aggccggcta
     2581 ccgccgctcc ccgcaggaca tccagccggt cctcgcgacg ctggagctcg acctgggcgc
     2641 ggacgaggac gcgctgatgg ccgggctcga gaaggacacc cgttggagcg tgcgacaggc
     2701 cgcgaagaag ggcgtcacgg tccgcgaggt cgccgacgac gcggggctgc gcgcgttcta
     2761 cgagctctac gcggcgaccg gcgagcgtgc cggcttcatc acgcgcacct ggacgtatta
     2821 ccgcgagaca tggggcagac tggtcgcggc agggctcgcg acggtcaggc tcgcagacgt
     2881 cgagggcgag gccgtggccg gcgcgatggt ctggcgctgt ggcgaccgcg ccctctatca
     2941 gacggcc
//
LOCUS       DASTZR010000513         2560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2474
            k141_338914, whole genome shotgun sequence.
ACCESSION   DASTZR010000513 DASTZR010000000
VERSION     DASTZR010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960931
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:21:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,247
            Genes (coding)                    :: 2,230
            CDSs (with protein)               :: 2,230
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338914"
                     /isolate="SMAG_U2474"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VFV20_11330"
     CDS             complement(<1..495)
                     /locus_tag="VFV20_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEU5288985.1"
                     /translation="MSFIGRVFDSNEGELRRLRKVAQRVSALEDAAKALSDDELRRRT
                     ELFRERLAKGEELDDLLPEAFAAVREAARRTLQQRHFDVQLLGGMALHKGHIAEMKTG
                     EGKTLVASLALYVNALAGKGAHLVTTNDYLAKTGAGWMAPIYHALGMSVAYIAHDKSA
                     VYDPA"
     gene            complement(506..1504)
                     /locus_tag="VFV20_11335"
     CDS             complement(506..1504)
                     /locus_tag="VFV20_11335"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU5288986.1"
                     /translation="MYGGLAVYTGNAHPQFAKDVCEALEMPLGNADVFKFANDNIFVK
                     INENVREQDVFILQSLTAHPGVGVSDAIMEMLIMIDACKRASAGRITAVMPYYAYGRS
                     DKKDQPRVPITARLLADLITVAGADRFLTIDMHQMQIQGFFGIPGDELSARNLLVEYL
                     RSIVETDKDSAALVVPDLGYANQARRIAELLDIPLVFMQKTHRDNSGRSEVVGVIGDI
                     DRRRCIVIDDEIDRGTTMVNTIRVLQERGVSEIWAACTHAVLSGDAPERLLGSGIVEL
                     VTTDTCPLSSPVRNDPRIRVLSVAPLFAEAIRRIHEGESVGALFNPPGLQLPLQVR"
     gene            1599..1772
                     /locus_tag="VFV20_11340"
     CDS             1599..1772
                     /locus_tag="VFV20_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5670 family protein"
                     /protein_id="HEU5288987.1"
                     /translation="MTMAAGHRVRWILIWLLIAGWFVALAANIGGNGAHLLLLAAIAL
                     LVYELLIPEETPS"
     gene            complement(1747..>2560)
                     /locus_tag="VFV20_11345"
     CDS             complement(1747..>2560)
                     /locus_tag="VFV20_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5288988.1"
                     /translation="RAMSGGSAEGGAPRPALFDHHIPTDRSDGTVSLAARAETVKVRP
                     HGVSDHFPWGDRLRTDDDVLRYVDDASRLGLRVGIEYDLGVAPELRQTTRDALHYVIG
                     ALHQLELADGEWIRFDEAGAYLKGRIRAFSQVERFLDAGLQERIRERTLAVVRAGIER
                     DGIDILGHATMGPLGALGDPERAYPAEWQERLIRLCVDADVAIEVNECYGVPHREFLV
                     RARQLGARFAVGSDTHFALAPLDRTAEMIAAAGLARDRFLAGQRVRTASPRG"
BASE COUNT          367 a          874 c          901 g          418 t
ORIGIN      
        1 cgccgggtcg tacaccgccg acttgtcgtg cgcgatgtac gcgacgctca tgccgagcgc
       61 gtgatagatc ggcgccatcc agccggcgcc ggtcttcgcc aggtagtcgt tcgtcgtgac
      121 caggtgcgcg cccttgcccg cgagcgcatt gacgtacagc gccagcgacg cgacgagcgt
      181 ctttccttca ccggtcttca tctcggcgat gtggcccttg tggagcgcca tgccgcccag
      241 cagctggacg tcgaagtggc gctgctgcag cgtgcggcgc gccgcctcgc ggaccgccgc
      301 gaatgcctcg gggaggaggt cgtcgagctc ctcccccttc gcgaggcgct cccggaagag
      361 ctcggtccgc cggcgcagct cgtcgtccga cagcgccttc gccgcgtcct cgagcgcgct
      421 cacgcgctgg gccaccttgc gcaggcggcg aagctctcct tcgttggagt cgaagacccg
      481 gccgatgaag ctcacgacgc ggtggttatc tgacctggag cggcagctgc aaccccggtg
      541 gattgaagag cgctccgacc gactccccct cgtggatgcg acggatcgcc tcggcgaaca
      601 ggggagccac ggagagcacg cggatacgcg ggtcgttgcg gactggcgag ctcaacgggc
      661 aggtgtctgt ggtcacgagt tcgacgatac cgctcccgag gaggcgctcc ggcgcgtcgc
      721 ccgacagcac ggcgtgcgtg caggccgccc agatctcgga gacgccacgc tcctggagga
      781 cgcggatggt gttcaccatc gtcgtcccgc ggtcgatctc gtcgtcgatc acgatgcagc
      841 ggcgccggtc gatgtcgccg atgacgccga cgacctcgga cctgccgctg ttgtcgcgat
      901 gcgtcttctg catgaagacc agcgggatgt cgagcagctc ggcgatgcgg cgcgcctggt
      961 tcgcgtagcc gagatcggga acgacgagcg ccgcgctgtc cttgtccgtc tcgacgatcg
     1021 agcgcaggta ctccaccagg aggttgcgcg cggacagctc gtcgccgggg atgccgaaga
     1081 agccctggat ctgcatctgg tgcatgtcga tggtcaggaa gcggtcggcg ccggcgaccg
     1141 tgatgaggtc ggcgaggagg cgcgcggtga tcggaacgcg gggctggtcc ttcttgtcgc
     1201 tccgtccgta cgcgtagtac ggcatcacgg ccgtgatgcg acccgccgac gcccgcttgc
     1261 aggcgtcgat catgatcagc atctccatga tcgcgtcgga gacacccacg ccagggtggg
     1321 cggtgagcga ctggaggatg aacacgtcct gctcgcggac gttctcgttg atcttcacga
     1381 agatgttgtc gttcgcgaac ttgaacacgt cggcgttccc gaggggcatc tcgagcgcct
     1441 cgcaaacgtc cttggcgaac tgtgggtggg cgttcccggt gtagacggcg agtcccccgt
     1501 acacaggctc cccctccgtg ctcctcgttc gcgggcccgc gagcactcga agtatatggg
     1561 ggcgaggccg tccctcgtcg ctcgctatgc tccgagaaat gacgatggcc gcagggcatc
     1621 gcgtccgctg gatcctcatc tggctgctca tcgctgggtg gttcgtggcg ctcgcggcga
     1681 acatcggcgg caacggcgcc caccttctgc tgctcgcggc gatcgcgctc ctggtctacg
     1741 agctgctcat ccccgaggag acgccgtcct gacccgctga cccgcgagga agcggtcccg
     1801 ggcgagaccg gcggcggcga tcatctcggc ggtccggtcc agcggcgcga gcgcgaagtg
     1861 cgtgtccgac ccgaccgcga agcgtgcgcc gagctgccgc gcacgaacga ggaactcgcg
     1921 gtgtggcacg ccgtagcact cgttcacctc gatcgcgacg tccgcgtcga cgcagagcct
     1981 gatgaggcgc tcctgccact cggcgggata cgcgcgctcg gggtcgccga gggcgccgag
     2041 cggccccatc gtggcgtggc cgaggatgtc gatgccgtcc cgctcgatgc ccgcacgcac
     2101 gaccgcgagc gtccgctcgc ggatgcgctc ctgcaggccg gcgtcgagga agcgctcgac
     2161 ctgcgagaac gcgcggatgc ggcccttgag gtacgcgccg gcctcgtcga agcggatcca
     2221 ctcgccgtcc gcgagctcga gttggtgcag cgcgccgatc acgtagtgca gcgcgtcgcg
     2281 ggtcgtctgg cgaagctcgg gcgcgacgcc gaggtcgtat tcgatgccca cccgcagacc
     2341 gagccgcgac gcgtcgtcga catagcggag gacgtcgtcg tcggtgcgca gcctgtcgcc
     2401 ccacgggaag tgatcggaca ccccgtgcgg ccgcaccttc accgtctcgg cgcgcgccgc
     2461 gaggctcacg gtgccgtccg aacgatcggt ggggatgtgg tggtcgaaca gcgctgggcg
     2521 gggggcgccg ccctccgccg accccccgct catggctctg
//
